LOCUS       DAJTUI010000001       123662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTUI010000001 DAJTUI010000000
VERSION     DAJTUI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 123662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 123662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..123662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.001"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            145..1578
                     /gene="cscA"
                     /locus_tag="OO464_000001"
     CDS             145..1578
                     /gene="cscA"
                     /locus_tag="OO464_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR6225318.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1586..2644)
                     /locus_tag="OO464_000002"
     CDS             complement(1586..2644)
                     /locus_tag="OO464_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6225319.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2812..3030
                     /locus_tag="OO464_000003"
                     /pseudo
     CDS             2812..3030
                     /locus_tag="OO464_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3048..4376
                     /gene="dsdA"
                     /locus_tag="OO464_000004"
     CDS             3048..4376
                     /gene="dsdA"
                     /locus_tag="OO464_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR6225320.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4484..6022)
                     /gene="emrY"
                     /locus_tag="OO464_000005"
     CDS             complement(4484..6022)
                     /gene="emrY"
                     /locus_tag="OO464_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6225321.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6022..7185)
                     /gene="emrK"
                     /locus_tag="OO464_000006"
     CDS             complement(6022..7185)
                     /gene="emrK"
                     /locus_tag="OO464_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6225322.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7601..8215
                     /gene="evgA"
                     /locus_tag="OO464_000007"
     CDS             7601..8215
                     /gene="evgA"
                     /locus_tag="OO464_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6225323.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8220..11813
                     /gene="evgS"
                     /locus_tag="OO464_000008"
     CDS             8220..11813
                     /gene="evgS"
                     /locus_tag="OO464_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6225324.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11869..13014)
                     /gene="yfdE"
                     /locus_tag="OO464_000009"
     CDS             complement(11869..13014)
                     /gene="yfdE"
                     /locus_tag="OO464_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6225325.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13088..14032)
                     /gene="yfdV"
                     /locus_tag="OO464_000010"
     CDS             complement(13088..14032)
                     /gene="yfdV"
                     /locus_tag="OO464_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6225326.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14102..15796)
                     /gene="oxc"
                     /locus_tag="OO464_000011"
     CDS             complement(14102..15796)
                     /gene="oxc"
                     /locus_tag="OO464_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6225327.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15850..17100)
                     /gene="frc"
                     /locus_tag="OO464_000012"
     CDS             complement(15850..17100)
                     /gene="frc"
                     /locus_tag="OO464_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6225328.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17613..18236)
                     /locus_tag="OO464_000013"
     CDS             complement(17613..18236)
                     /locus_tag="OO464_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6225329.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18533..18808
                     /locus_tag="OO464_000014"
     CDS             18533..18808
                     /locus_tag="OO464_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225330.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18885..19127)
                     /locus_tag="OO464_000015"
     CDS             complement(18885..19127)
                     /locus_tag="OO464_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6225331.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19480..20400
                     /gene="lpxP"
                     /locus_tag="OO464_000016"
     CDS             19480..20400
                     /gene="lpxP"
                     /locus_tag="OO464_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6225332.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20755..20826
                     /gene="ypdK"
                     /locus_tag="OO464_000017"
     CDS             20755..20826
                     /gene="ypdK"
                     /locus_tag="OO464_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6225333.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20891..22129)
                     /gene="alaC"
                     /locus_tag="OO464_000018"
     CDS             complement(20891..22129)
                     /gene="alaC"
                     /locus_tag="OO464_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6225334.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22506..23992
                     /locus_tag="OO464_000019"
                     /pseudo
     CDS             22506..23992
                     /locus_tag="OO464_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24007..24741
                     /gene="ypdB"
                     /locus_tag="OO464_000020"
     CDS             24007..24741
                     /gene="ypdB"
                     /locus_tag="OO464_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR6225335.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24754..25611
                     /locus_tag="OO464_000021"
                     /pseudo
     CDS             24754..25611
                     /locus_tag="OO464_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25614..28109)
                     /gene="ptsP"
                     /locus_tag="OO464_000022"
     CDS             complement(25614..28109)
                     /gene="ptsP"
                     /locus_tag="OO464_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6225336.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28134..29171)
                     /gene="ypdE"
                     /locus_tag="OO464_000023"
     CDS             complement(28134..29171)
                     /gene="ypdE"
                     /locus_tag="OO464_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6225337.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29171..30254)
                     /gene="ypdF"
                     /locus_tag="OO464_000024"
                     /pseudo
     CDS             complement(29171..30254)
                     /gene="ypdF"
                     /locus_tag="OO464_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30269..31516)
                     /locus_tag="OO464_000025"
     CDS             complement(30269..31516)
                     /locus_tag="OO464_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6225338.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31538..31864)
                     /locus_tag="OO464_000026"
     CDS             complement(31538..31864)
                     /locus_tag="OO464_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6225339.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32083..33048)
                     /gene="glk"
                     /locus_tag="OO464_000027"
     CDS             complement(32083..33048)
                     /gene="glk"
                     /locus_tag="OO464_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6225340.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33252..34508
                     /locus_tag="OO464_000028"
     CDS             33252..34508
                     /locus_tag="OO464_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6225341.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34623..34949
                     /locus_tag="OO464_000029"
     CDS             34623..34949
                     /locus_tag="OO464_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6225342.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35090..36328)
                     /locus_tag="OO464_000030"
     CDS             complement(35090..36328)
                     /locus_tag="OO464_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6225343.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36664..37866
                     /gene="nupC"
                     /locus_tag="OO464_000031"
     CDS             36664..37866
                     /gene="nupC"
                     /locus_tag="OO464_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6225344.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(37916..40028)
                     /locus_tag="OO464_000032"
                     /pseudo
     CDS             complement(37916..40028)
                     /locus_tag="OO464_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40236..40311)
                     /locus_tag="OO464_000033"
     tRNA            complement(40236..40311)
                     /locus_tag="OO464_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40276..40278),aa:Ala,seq:ggc)
     gene            complement(40351..40426)
                     /locus_tag="OO464_000034"
     tRNA            complement(40351..40426)
                     /locus_tag="OO464_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40391..40393),aa:Ala,seq:ggc)
     gene            40647..41006
                     /locus_tag="OO464_000035"
     CDS             40647..41006
                     /locus_tag="OO464_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6225345.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41008..41400
                     /locus_tag="OO464_000036"
     CDS             41008..41400
                     /locus_tag="OO464_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6225346.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41452..42867)
                     /gene="gltX"
                     /locus_tag="OO464_000037"
     CDS             complement(41452..42867)
                     /gene="gltX"
                     /locus_tag="OO464_000037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6225347.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43126..43201
                     /locus_tag="OO464_000038"
     tRNA            43126..43201
                     /locus_tag="OO464_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43159..43161,aa:Val,seq:tac)
     gene            43246..43321
                     /locus_tag="OO464_000039"
     tRNA            43246..43321
                     /locus_tag="OO464_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43279..43281,aa:Val,seq:tac)
     gene            43326..43401
                     /locus_tag="OO464_000040"
     tRNA            43326..43401
                     /locus_tag="OO464_000040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43359..43361,aa:Lys,seq:ttt)
     gene            43432..43557
                     /locus_tag="OO464_000041"
     ncRNA           43432..43557
                     /ncRNA_class="other"
                     /locus_tag="OO464_000041"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43677..44018
                     /locus_tag="OO464_000042"
     CDS             43677..44018
                     /locus_tag="OO464_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6225348.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44009..44935)
                     /locus_tag="OO464_000043"
     CDS             complement(44009..44935)
                     /locus_tag="OO464_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6225349.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45025..46023
                     /locus_tag="OO464_000044"
     CDS             45025..46023
                     /locus_tag="OO464_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6225350.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46020..46238)
                     /locus_tag="OO464_000045"
     CDS             complement(46020..46238)
                     /locus_tag="OO464_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6225351.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46240..48255)
                     /gene="ligA"
                     /locus_tag="OO464_000046"
     CDS             complement(46240..48255)
                     /gene="ligA"
                     /locus_tag="OO464_000046"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6225352.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48326..49312)
                     /gene="zipA"
                     /locus_tag="OO464_000047"
     CDS             complement(48326..49312)
                     /gene="zipA"
                     /locus_tag="OO464_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6225353.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49542..50303
                     /gene="cysZ"
                     /locus_tag="OO464_000048"
     CDS             49542..50303
                     /gene="cysZ"
                     /locus_tag="OO464_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6225354.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50488..51459
                     /gene="cysK"
                     /locus_tag="OO464_000049"
     CDS             50488..51459
                     /gene="cysK"
                     /locus_tag="OO464_000049"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6225355.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51843..52100
                     /gene="ptsH"
                     /locus_tag="OO464_000050"
     CDS             51843..52100
                     /gene="ptsH"
                     /locus_tag="OO464_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6225356.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52145..53872
                     /gene="ptsI"
                     /locus_tag="OO464_000051"
     CDS             52145..53872
                     /gene="ptsI"
                     /locus_tag="OO464_000051"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6225357.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53913..54422
                     /gene="crr"
                     /locus_tag="OO464_000052"
     CDS             53913..54422
                     /gene="crr"
                     /locus_tag="OO464_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6225358.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54464..55315)
                     /gene="pdxK"
                     /locus_tag="OO464_000053"
                     /gene_synonym="thiJ"
     CDS             complement(54464..55315)
                     /gene="pdxK"
                     /locus_tag="OO464_000053"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR6225359.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55420..55794
                     /locus_tag="OO464_000054"
     CDS             55420..55794
                     /locus_tag="OO464_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6225360.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55827..56550
                     /locus_tag="OO464_000055"
                     /pseudo
     CDS             55827..56550
                     /locus_tag="OO464_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56754..57665)
                     /gene="cysM"
                     /locus_tag="OO464_000056"
     CDS             complement(56754..57665)
                     /gene="cysM"
                     /locus_tag="OO464_000056"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6225361.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57799..58896)
                     /gene="cysA"
                     /locus_tag="OO464_000057"
     CDS             complement(57799..58896)
                     /gene="cysA"
                     /locus_tag="OO464_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6225362.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58886..59761)
                     /gene="cysW"
                     /locus_tag="OO464_000058"
     CDS             complement(58886..59761)
                     /gene="cysW"
                     /locus_tag="OO464_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6225363.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59761..60594)
                     /gene="cysT"
                     /locus_tag="OO464_000059"
     CDS             complement(59761..60594)
                     /gene="cysT"
                     /locus_tag="OO464_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6225364.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60594..61610)
                     /gene="cysP"
                     /locus_tag="OO464_000060"
     CDS             complement(60594..61610)
                     /gene="cysP"
                     /locus_tag="OO464_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6225365.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(61914..62705)
                     /gene="ucpA"
                     /locus_tag="OO464_000061"
     CDS             complement(61914..62705)
                     /gene="ucpA"
                     /locus_tag="OO464_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6225366.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62892..63692)
                     /gene="murR"
                     /locus_tag="OO464_000062"
     CDS             complement(62892..63692)
                     /gene="murR"
                     /locus_tag="OO464_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6225367.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63856..64752
                     /gene="murQ"
                     /locus_tag="OO464_000063"
     CDS             63856..64752
                     /gene="murQ"
                     /locus_tag="OO464_000063"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6225368.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64756..66180
                     /gene="murP"
                     /locus_tag="OO464_000064"
     CDS             64756..66180
                     /gene="murP"
                     /locus_tag="OO464_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR6225369.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66360..67259)
                     /gene="yfeX"
                     /locus_tag="OO464_000065"
     CDS             complement(66360..67259)
                     /gene="yfeX"
                     /locus_tag="OO464_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6225370.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67355..67930)
                     /locus_tag="OO464_000066"
     CDS             complement(67355..67930)
                     /locus_tag="OO464_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6225371.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(67991..68440)
                     /locus_tag="OO464_000067"
     CDS             complement(67991..68440)
                     /locus_tag="OO464_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6225372.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68427..68852)
                     /locus_tag="OO464_000068"
     CDS             complement(68427..68852)
                     /locus_tag="OO464_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6225373.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69066..69935
                     /gene="amiA"
                     /locus_tag="OO464_000069"
     CDS             69066..69935
                     /gene="amiA"
                     /locus_tag="OO464_000069"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6225374.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            69939..70838
                     /gene="hemF"
                     /locus_tag="OO464_000070"
     CDS             69939..70838
                     /gene="hemF"
                     /locus_tag="OO464_000070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6225375.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70844..71896)
                     /gene="eutR"
                     /locus_tag="OO464_000071"
                     /pseudo
     CDS             complement(70844..71896)
                     /gene="eutR"
                     /locus_tag="OO464_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(71942..72442)
                     /gene="eutK"
                     /locus_tag="OO464_000072"
     CDS             complement(71942..72442)
                     /gene="eutK"
                     /locus_tag="OO464_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR6225376.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72455..73114)
                     /gene="eutL"
                     /locus_tag="OO464_000073"
     CDS             complement(72455..73114)
                     /gene="eutL"
                     /locus_tag="OO464_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6225377.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74296..75087
                     /locus_tag="OO464_000074"
     CDS             74296..75087
                     /locus_tag="OO464_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225378.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75807..76202
                     /locus_tag="OO464_000075"
     CDS             75807..76202
                     /locus_tag="OO464_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6225379.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76220..76483
                     /locus_tag="OO464_000076"
     CDS             76220..76483
                     /locus_tag="OO464_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225380.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76506..77048
                     /locus_tag="OO464_000077"
     CDS             76506..77048
                     /locus_tag="OO464_000077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225381.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77187..77474
                     /locus_tag="OO464_000078"
     CDS             77187..77474
                     /locus_tag="OO464_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225382.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77513..77719
                     /locus_tag="OO464_000079"
     CDS             77513..77719
                     /locus_tag="OO464_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225383.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78031..78516
                     /locus_tag="OO464_000080"
     CDS             78031..78516
                     /locus_tag="OO464_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6225384.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78497..78775
                     /locus_tag="OO464_000081"
     CDS             78497..78775
                     /locus_tag="OO464_000081"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225385.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            78768..80228
                     /locus_tag="OO464_000082"
     CDS             78768..80228
                     /locus_tag="OO464_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6225386.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80262..81071
                     /gene="murI"
                     /locus_tag="OO464_000083"
     CDS             80262..81071
                     /gene="murI"
                     /locus_tag="OO464_000083"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6225387.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81190..81884)
                     /locus_tag="OO464_000084"
     CDS             complement(join(81190..81638,81638..81884))
                     /locus_tag="OO464_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6225388.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82403..83776)
                     /gene="eutA"
                     /locus_tag="OO464_000085"
     CDS             complement(82403..83776)
                     /gene="eutA"
                     /locus_tag="OO464_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6225389.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83751..84230)
                     /gene="eutP"
                     /locus_tag="OO464_000086"
     CDS             complement(83751..84230)
                     /gene="eutP"
                     /locus_tag="OO464_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR6225390.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(84243..84578)
                     /gene="eutS"
                     /locus_tag="OO464_000087"
     CDS             complement(84243..84578)
                     /gene="eutS"
                     /locus_tag="OO464_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR6225391.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(84871..87150)
                     /gene="maeB"
                     /locus_tag="OO464_000088"
     CDS             complement(84871..87150)
                     /gene="maeB"
                     /locus_tag="OO464_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR6225392.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            87439..88389
                     /gene="tal"
                     /locus_tag="OO464_000089"
     CDS             87439..88389
                     /gene="tal"
                     /locus_tag="OO464_000089"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6225393.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            88409..90412
                     /gene="tkt"
                     /locus_tag="OO464_000090"
     CDS             88409..90412
                     /gene="tkt"
                     /locus_tag="OO464_000090"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6225394.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(90515..91558)
                     /locus_tag="OO464_000091"
     CDS             complement(90515..91558)
                     /locus_tag="OO464_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6225395.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(91684..92259)
                     /gene="nudK"
                     /locus_tag="OO464_000092"
     CDS             complement(91684..92259)
                     /gene="nudK"
                     /locus_tag="OO464_000092"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6225396.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(92327..94306)
                     /gene="aegA"
                     /locus_tag="OO464_000093"
     CDS             complement(92327..94306)
                     /gene="aegA"
                     /locus_tag="OO464_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR6225397.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            94512..96229
                     /gene="narQ"
                     /locus_tag="OO464_000094"
                     /pseudo
     CDS             94512..96229
                     /gene="narQ"
                     /locus_tag="OO464_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            96431..99544
                     /gene="acrD"
                     /locus_tag="OO464_000095"
     CDS             96431..99544
                     /gene="acrD"
                     /locus_tag="OO464_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR6225398.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(99643..99702)
                     /gene="ypfM"
                     /locus_tag="OO464_000096"
     CDS             complement(99643..99702)
                     /gene="ypfM"
                     /locus_tag="OO464_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6225399.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            100083..100439
                     /locus_tag="OO464_000097"
     CDS             100083..100439
                     /locus_tag="OO464_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6225400.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            100443..101570
                     /gene="dapE"
                     /locus_tag="OO464_000098"
     CDS             100443..101570
                     /gene="dapE"
                     /locus_tag="OO464_000098"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6225401.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            101598..101798
                     /locus_tag="OO464_000099"
     CDS             101598..101798
                     /locus_tag="OO464_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6225402.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(101908..102606)
                     /gene="ypfH"
                     /locus_tag="OO464_000100"
     CDS             complement(101908..102606)
                     /gene="ypfH"
                     /locus_tag="OO464_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6225403.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(102680..104695)
                     /gene="tmcA"
                     /locus_tag="OO464_000101"
     CDS             complement(102680..104695)
                     /gene="tmcA"
                     /locus_tag="OO464_000101"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6225404.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(104710..105573)
                     /locus_tag="OO464_000102"
     CDS             complement(104710..105573)
                     /locus_tag="OO464_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6225405.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(105741..106454)
                     /gene="purC"
                     /locus_tag="OO464_000103"
     CDS             complement(105741..106454)
                     /gene="purC"
                     /locus_tag="OO464_000103"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6225406.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(106667..107701)
                     /gene="bamC"
                     /locus_tag="OO464_000104"
     CDS             complement(106667..107701)
                     /gene="bamC"
                     /locus_tag="OO464_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6225407.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(107718..108596)
                     /gene="dapA"
                     /locus_tag="OO464_000105"
     CDS             complement(107718..108596)
                     /gene="dapA"
                     /locus_tag="OO464_000105"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6225408.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            108742..109314
                     /locus_tag="OO464_000106"
     CDS             108742..109314
                     /locus_tag="OO464_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6225409.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            109314..109784
                     /gene="bcp"
                     /locus_tag="OO464_000107"
     CDS             109314..109784
                     /gene="bcp"
                     /locus_tag="OO464_000107"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6225410.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            110037..110654
                     /gene="hyfA"
                     /locus_tag="OO464_000108"
     CDS             110037..110654
                     /gene="hyfA"
                     /locus_tag="OO464_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6225411.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            110654..112671
                     /gene="hyfB"
                     /locus_tag="OO464_000109"
                     /pseudo
     CDS             110654..112671
                     /gene="hyfB"
                     /locus_tag="OO464_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            112682..113629
                     /gene="hyfC"
                     /locus_tag="OO464_000110"
     CDS             112682..113629
                     /gene="hyfC"
                     /locus_tag="OO464_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6225412.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            113646..115085
                     /gene="hyfD"
                     /locus_tag="OO464_000111"
     CDS             113646..115085
                     /gene="hyfD"
                     /locus_tag="OO464_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR6225413.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            115097..115747
                     /gene="hyfE"
                     /locus_tag="OO464_000112"
     CDS             115097..115747
                     /gene="hyfE"
                     /locus_tag="OO464_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR6225414.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            115752..117338
                     /gene="hyfF"
                     /locus_tag="OO464_000113"
     CDS             115752..117338
                     /gene="hyfF"
                     /locus_tag="OO464_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6225415.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            117328..119042
                     /gene="hyfG"
                     /locus_tag="OO464_000114"
                     /pseudo
     CDS             117328..119042
                     /gene="hyfG"
                     /locus_tag="OO464_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            119052..119597
                     /gene="hyfH"
                     /locus_tag="OO464_000115"
     CDS             119052..119597
                     /gene="hyfH"
                     /locus_tag="OO464_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6225416.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            119594..120352
                     /gene="hyfI"
                     /locus_tag="OO464_000116"
     CDS             119594..120352
                     /gene="hyfI"
                     /locus_tag="OO464_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6225417.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            120345..120758
                     /gene="hyfJ"
                     /locus_tag="OO464_000117"
     CDS             120345..120758
                     /gene="hyfJ"
                     /locus_tag="OO464_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6225418.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            120788..122617
                     /gene="hyfR"
                     /locus_tag="OO464_000118"
     CDS             120788..122617
                     /gene="hyfR"
                     /locus_tag="OO464_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR6225419.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            122618..122800
                     /locus_tag="OO464_000119"
     CDS             122618..122800
                     /locus_tag="OO464_000119"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCR6225420.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            122822..123662
                     /locus_tag="OO464_000120"
                     /pseudo
     CDS             122822..123662
                     /locus_tag="OO464_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
BASE COUNT        30754 a        31789 c        30308 g        30811 t
ORIGIN      
        1 ctaaaacatg catattcgat cacaaaacgc catagttaac gttaacattt gcgatattca
       61 tcgcatttat gaaagtaagg gactatattt ttataaaagt taacgttaat aattcaccaa
      121 atttgcttaa ccaggatgat taaaatgacg caatctcgat tgcatgcggc gcaaaacgca
      181 ctagcaaaac ttcatgagca ccggggtaac actttctatc cccattttca cctcgcgcct
      241 cctgccggat ggatgaacga tccaaacggc ctgatctggt ttaacgatcg ttatcacgcg
      301 ttttatcaac atcacccgat gagcgaacac tggggaccaa tgcactgggg acatgccacc
      361 agtgacgata tgatccactg gcagcatgag cctattgcgc tagcgctagg agacgataat
      421 gacaaagacg ggtgtttttc aggtagtgct gtcgatgaca atggtgtcct ctcacttatc
      481 tacaccggac acgtctggct cgatggtgca ggtaatgacg atgcaattcg cgaagtacaa
      541 tgtctggcta ccagtcggga tggtattcat ttcgagaaac agggtgtgat cctcactcca
      601 ccagaaggaa tcatgcactt ccgcgatcct aaagtgtggc gtgaagccga cacatggtgg
      661 atggtagtcg gggcgaaaga tccaggcaac acggggcaga tcctgcttta tcgcggcagt
      721 tcattgcgtg aatgggcctt cgatcgcgta ctggcccacg ctgatgcggg tgaaagctat
      781 atgtgggaat gtccggactt tttcagcctt ggcgatcagc attatctgat gttttccccg
      841 cagggaatga atgccgaggg atacagttac cgaaatcgct ttcaaagtgg cgtaataccc
      901 ggaatgtggt cgccaggacg actttttgca caatccgggc attttactga acttgataac
      961 gggcatgact tttatgcacc acaaagcttt ttagcgaagg atggtcggcg tattgttatc
     1021 ggctggatgg atatgtggga atcgccaatg ccctcaaaac gtgaaggatg ggcaggctgc
     1081 atgacgctgg cgcgcgagct atcagagagc aatggcaaac ttctacaacg cccggttcac
     1141 gaagctgagt cgttacgcca gcagcatcaa tctgtctctc cccgcagaat cagcaataaa
     1201 tatgttttgc aggaaaacgc gcaagcagtt gagattcagt tgcagtgggc gctgaagaac
     1261 agtgatgccg aacattacgg attacagctc ggcactggaa tgcggctgta tattgataac
     1321 caatctgagc gacttatttt gtggcggtat tacccacacg agaatttaga cggctaccgt
     1381 agtattcccc tcccgcagcg tgacacgctc gccctaagga tatttatcga tacatcatcc
     1441 gtggaagtat ttattaacga cggggaagcg gtgatgagta gtcgaatcta tccgcagcca
     1501 gaagaacggg aactgtcgct ttatgcctcc cacggagtgg ctgtgctgca acatggagca
     1561 ctctggctac tgggttaaca taatatcagg tggaacaacg gatcaacagc gggcaaggga
     1621 tccgcgtcac tcttccccct tcacgacctt caataatatg caatgcagct tcccgcccga
     1681 taatgtcatg tggaagctga attgtggtca gcggcggtaa aaacagatgc ccgacgccaa
     1741 ccagattatc aaagcccatt acagcgacat cctgcgggat acgtaccccc ttcgccaaaa
     1801 gaacctgata agccacaaag gctgcgcgat cgttaccaca tatcagaaca tcaaaatctg
     1861 gtttgcccga tttgaagtga gcattgagta aacttgcgag atcggtgtag tgatcatcac
     1921 ctgttgccat gtgaaattgt ttcacctcag ccagatctcg tccagcatca cgccaagcct
     1981 gctcaaatcc ctgccgacga taccctgttg ccaacgcact ttccggtagc cagaagcata
     2041 atggttgacg atagcccgcc gcgagcaaat gctgtgttga ttcatattgt gcagtgtaat
     2101 catcagggat ataactgggt aacgctgggt catccgccac acagttcgcc aatacaatat
     2161 tttcaccata cagagactca ggcagcgtga tatgtcgcag ccccattgta gtatagataa
     2221 tgccatccgg acggtgggca agcagctgac gtgccgcgcg ggcagcgtca tcttcagaaa
     2281 aaatattgat taaaaaacta ttccagccga actcgctggc ggtttgctca atggcaagca
     2341 gaatatcaac agagaaagga gtggtagccg tgtcctgcgc cagcacggcg agagtcgacg
     2401 gcttacgtcc ttgagcgcgc atcttacggg cggaaagatc aggaacataa ttcagggtct
     2461 ggattgcctg caatacgcgg tcacgcgttg caggacgcac agattctgca ttatgcatca
     2521 cccgggagac tgtcatcatc gacactcccg ccaggcgtgc gacatccttt aatgaagcca
     2581 tacccaagcc gtttgccgta aaacgggcac tgtagcagaa acagacgtca ctggcgagat
     2641 ccaacgccct atcacctgac acagcaatac aataaaaaat aacaataatt cccggacaat
     2701 tgtccccaat tccgcctctg ttctcgccaa cgagtaacca tataggcaag taagtaaaac
     2761 atgccaactg gcatcgccaa cctggagaag aaaatataaa tatggatcat tgtgctgcca
     2821 ctgtatcccg acatcagccc ggaaattatt gcgattgcta tcggttcagg tgcaattggc
     2881 tgcacgatcg ttacggactc gcttttctgg ctggtgaagc aatattgcgg cgctacgctc
     2941 aatgaaacat ttaaatacta tacgacagcg acatttatcg cttcagtcgt cgctctggcg
     3001 ggcacattcc tgctgtcatt tatcatctaa gcgcaaagag acgtactatg gaaaacgcta
     3061 aaatgaactc gctcatcgcc cagtatccgt tggtaaagga tctggttgct cttaaagaaa
     3121 ccacctggtt taatcctagc acgacctcat tggctgaagg tttaccttat gttggcctga
     3181 ccgaacagga tgttcaggac gcccatgcgc gcttatcccg ttttgcaccc tatctggcaa
     3241 aagcatttgc tgaaacagct gccactgggg ggattattga atcagaactg gttgccattc
     3301 cggctatgca aaaacggctg gaaaaggaat atcaacaacc gatcagcggg caactcctac
     3361 tgaaaaaaga tagccatttg cccatttccg gctctataaa agcacgcggc gggatttatg
     3421 aagtcctggc acatgcagaa aaactggctc tggaagcggg gttgctgacg cttgatgatg
     3481 actacagcaa actgctttct ccggagttta aacagttctt tagccaatac agcattgctg
     3541 tgggctcaac cggaaatctg gggttatcaa tcggcattat gagcgcccgc attggcttta
     3601 aggtgacagt tcatatgtct gctgatgccc gggcatggaa aaaagcgaaa ctgcgcagcc
     3661 atggcgttac ggtcgtggaa tatgagcaag attatggtgt tgccgtcgag gaaggacgta
     3721 aagcagcgca gtctgacccg aactgtttct ttattgatga cgaaaattcc cgcacgttgt
     3781 tccttgggta ttccgttgct ggccagcgtc ttaaagcgca atttgcccag caaggccgta
     3841 tcgtcgatgc tgataaccct ctgtttgtct atctgccgtg tggtgttggc ggtggtcctg
     3901 gtggcgtcgc attcgggctt aaactggcgt ttggcgatca tgttcactgc ttttttgccg
     3961 aaccaacgca ctccccttgt atgttgttag gcgtccatac aggattacac gatcagattt
     4021 ctgttcagga tattggtatc gacaacctta ccgcagcgga tggccttgca gttggtcgcg
     4081 catcatgctt tgtcgggcgg gcaatggagc gtctgctgga tggcttctat acccttagcg
     4141 atcaaaccat gtatgacatg cttggctggc tggcgcagga agaaggtatt cgtcttgaac
     4201 cttcagcact ggcgggtatg gccggacctc agcgcgtgtg tgcatcagta agttaccaac
     4261 agatgcacgg tttcagcgca gaacaattgc gtaatgccac tcatctggtg tgggcgacgg
     4321 gaggtggaat ggtgccggaa gaagagatga atcaatatct ggcaaaaggc cgttaataac
     4381 gtttcaacgc aacatcgcaa tcctttccct gggtgagcga tgctgccgat ggcgcagact
     4441 taagatcccc ggtcttaccc gctataaccc cctcctttta tcatcaccca acgcctttcg
     4501 ctgtaaacgg cggtttcgca aaccaaacca aaaccgtaag taagataaaa gcaatagccg
     4561 ccatacggaa aatctcattt gcagaaatag aaagtgactg ctgggttatt tcattattaa
     4621 tttcattaag aactcctgaa agcgaaccat agtatttatc cataatttgt gacgatgaat
     4681 taaacacggg gttaaattga tcgatggttg ctgtcaactg actatggtgt aacgattcgc
     4741 gtcgtcccca cagcgtcatt gtcaacgacg taccaactga tcctgacaag gtacgaaaaa
     4801 aattactcat actcgaggca ttggcaaatt tattatctgg caagcctgaa aacgaaatcg
     4861 ttgttaaggg taaaaagaaa caggcaacgg cgaatccctg gaaaaactgt ggcaaaatga
     4921 tgcctgtaaa atcaatcgtt ggcataaatg tcacagaacg ccagtaatag caaaccgcat
     4981 acatcaaaaa actgaatgtc actaacaccc gcatgtctat tttattgcca taacgtccta
     5041 tcaaaggtga aatcaatagt ggcatgatgc cgatgggcgc ataagcaagc ccggcccata
     5101 tcgcattata ccccatcgtt ttctggagta actgcggcat aaggacgatc gctccagagt
     5161 aaaataaata cgcgcaggtg atactcacaa taccaatggt gaaattacgg gacttaaaca
     5221 aactgagatc aagaatcggg ttctctgagg tcgattccca aatgactaaa gagatcagag
     5281 aaataactga tactactgtt aatattatta ttgtactcga gttgaaccaa tccagatcgc
     5341 gccctttatc aagcataatt tgcaagccac caacaccgag cactaacagg gtcagtcttg
     5401 gtagattcat ttttaccggt gaagtttcag tttctcttcc tttaagtaag gttaagcata
     5461 atgtcaggac gataatcccc atagggacat tgattaaaaa tatccaaccc cagctaaagt
     5521 tatcgcaaat atagccgccc aatatcggcc cacatatcgg agcgataatc acggtcattg
     5581 accataatgc cagagcaaat gttctttttt ctggtggata attccttaat aataaactct
     5641 gtgacagtgg aattaacggc cccgccatta atccctgaac gactctaaaa aatatcagca
     5701 catcaagatt ggtcgataaa ctacacatta aggaagacag cgaaaaaaaa gtgactgaaa
     5761 gtaaaaataa ccttaattcg cctattcttt gtgccagcct gccggtaaca gggatcgcaa
     5821 tggcatttgc tacgccaaac gaggtgataa cccaggtgcc ttcgtctgtt gatgctccca
     5881 gaaagccaga tattgtcggt attgcgacgt tagaaatagt ggaatccaac atttgcataa
     5941 acgtcgctaa tgacaatgca atagtaacgc accataacgt cccaccggtt aatggtgccg
     6001 gagttgattt agtgattgcc attaaagttg tccattatgc gaaataatgt tgctaatttc
     6061 tttttctatc ggactggtat cgataactaa agccttactg gtataagcag gcatggaggt
     6121 cacggttgaa gccagatcag gcatctcagc aatgtcttcg tccttcgtat caatagttgc
     6181 agtcatcgat aaaccaatgc gcaaggggtg ttccatgagt tcttttggat caagagaaac
     6241 ttcaaccggt acacgctgaa cgattttgat ccagttccct gtcgcatttt gtgcaggtaa
     6301 taaggagaac gcattgccgg ttcccatatt gatccctgtc acccgaccat gaaacacaac
     6361 attttcacca taaagatcgc tgataatatt gactgattga ccaatccgta catccgtgag
     6421 ttgtgtttct ttaaagttgg cattaaccca catttgacgt gccggtacga cagccattaa
     6481 cgattgtccg gaactcactg tttcgccgac ctgaacactt cgctgggcaa tatagccagt
     6541 aaccggactc cttatatccg tacgtttaag caccaaccag gcttctttgg ttgcatccgc
     6601 cgcttcaact acttgtggct gacggtttaa tggtgtgttc attactaaag ctttattcgc
     6661 tttataagcc tggatagcgg cattcagtgc cgctttgcta cttattaacg tatctttggt
     6721 atgctccagc gtttcttttg aaataacccc ctgcttcgct aaaggcacac gacggttata
     6781 atcttctaaa gattgttgat actgaatacg tgctgaagcg acttcggcac tgtattgttt
     6841 atcctgtaag tacagtttat tcgtttgccg aacaatattt gccagattat ttttagcttt
     6901 attgagtgcg atagtggcat cagttttatc cagtgaaact aaaatgtcac cctgtcgaac
     6961 gtagttcgta tctttatgat taactacagt gacactacct gagacttgtg cagaaattgg
     7021 atctgcattc cccgtgacat aggcgtcatc tgtactaatc atgtcttcta attccattga
     7081 ccaataggca taggcacctg aaaacgcaat aaataaaact atcaccaata aagaaaagta
     7141 tttttttctg ttagactgtt ttttatttga attaatctgt tccactatta tctctcattt
     7201 cgcatagatg aaatttatga attgaatgaa aagagtattc attcttcata tcaaacacag
     7261 tgcatgtgtt gcacattatc ccaggaacgt tggaggccta atttaaatga ggtgcaaaaa
     7321 aaacaacaga ggactaaacc gtggcttttg caatacaatt cttacgcctg taggattagt
     7381 gagaagactt atagtgctaa cttgaaacta taaatcatcg gtataatccc tgattttatt
     7441 gttgacattt catttatgcc gactatttat atggtatact tgtcgaatta tcttaaagga
     7501 aactcagatt ttcttatttt tattgagaaa atgagatgac gccttatgtc tgtattacta
     7561 cagggagaag ggagatgctt cattgcaaag ggaataatct atgaacgcaa taattattga
     7621 tgaccatcct cttgctatcg cagcaattcg taatttattg atcaaaaacg atattgaaat
     7681 cttagcggag ttaactgaag gcggaagcgc cgttcagcgg gtggaaacac ttaagcctga
     7741 tatcgtcatc attgatgtcg atatccccgg agttaacggt atccaggtgt tagaaacgct
     7801 gaggaagcgc caatatagcg gaattattat tatcgtctcc gctaaaaatg accattttta
     7861 cgggaaacat tgtgctgatg ctggcgctaa tggtttcgtg agtaaaaaag aaggcatgaa
     7921 caatatcatt gcggctattg aagctgcaaa aaatggctac tgctatttcc ccttctctct
     7981 caaccggttt gttggaagtt taacgtccga ccagcaaaaa ctcgactcct tatcgaaaca
     8041 agaaattagt gtcatgcggt atattcttga tggcaaggat aataatgaca ttgcggaaaa
     8101 aatgttcatc agcaacaaaa ctgtcagcac gtataaaagt cgcctgatgg aaaaattaga
     8161 atgtaaatca ctgatggatc tttacacatt cgcgcaacgt aacaaaatcg gctaatcaca
     8221 tgaagttttt accctatttt tttcttctct gttgtggtct ttggtcgacc ataagtttcg
     8281 cagacgaaga ttacatcgaa tatcgtggca tcagtagtaa caaccgtgtc acacttgatc
     8341 cagtacgtct gagcaacaag gaattacgtt ggttagcgag caaaaaaaat cttgtgattg
     8401 cagtacacaa gtcccaaacg gctaccttgt tgcataccga ttcgcagcaa caggttcgtg
     8461 gtattaatgc tgattattta aatcttttaa aaagagcgtt aaatatcaaa ttaacactcc
     8521 gggaatacgc agatcatcaa aaagcaatgg acgcgcttgc agaaggtgaa gtcgatatag
     8581 tgttatcaca tttagttact tcgccgcctc ttaataatga cattgctgca accaaaccat
     8641 tgataattac ctttccggcg ctggtaacca cccttcacga ctcaatgcga ccgcttacct
     8701 caccaaaacc agtaaatatt gctcgggtag caaattaccc cccagacgag gtaattcatc
     8761 aatcatttcc aaaagcaaca attatctctt ttacaaattt atatcaggca ttagcatccg
     8821 tctcagctgg gcacaatgat tactttattg gtagtaacat cattaccagc agtatgattt
     8881 cccgctattt cactcactcc ttaaatgtag tgaaatatta taactcgccg cgtcaatata
     8941 attttttctt gaccagaaaa gaatctgtca ttcttaatga agtactcaat agatttgttg
     9001 atgctttaac aaatgaagtt cgctatgaag tatcacaaaa ttggcttgat acaggaaacc
     9061 tggcctttct gaacaaacca ttagaactca ctgaacatga aaaacagtgg attaagcagc
     9121 atcccaattt aaaggtgctg gaaaatcctt actcgccccc ctattctatg acggatgaaa
     9181 atggctcggt tcggggcgtt atgggggaca ttcttaatat tattaccttg caaacaggtt
     9241 taaatttttc tccgatcacc gtttcacaca atatccatgc tggaacacaa cttagccccg
     9301 gaggatggga tataatacct ggcgctattt atagtgaaga tcgagaaaat aatgttttat
     9361 ttgctgaagc cttcataaca acgccttacg tttttgtcat gcaaaaagcg cctgacagtg
     9421 aacaaacatt aaaaaaagga atgaaagttg ccattccata ttattatgag ctgcattcgc
     9481 aattaaaaga gatgtatccg gaggttgaat ggatacaggt cgataatgcc agcgctgcat
     9541 ttcacaagat taaggaaggt gaacttgatg ctctggtcgc gacacagcta aattcgcgtt
     9601 acatgatcga tcattactat cctaatgaac tttatcattt tcttattcct ggcgttccga
     9661 atgcatcgct ttcgttcgct tttcctcgcg gagaaccgga acttaaggat attattaata
     9721 aagcactgaa tgcaattccc ccaagcgaag ttctgcgcct gacggaaaaa tggattaaaa
     9781 tgcccaatgt gaccattgac acatgggacc tatatagcga gcaattttat attgttacga
     9841 cattatccgt tttattagtt ggcagtagcc ttttatgggg attctacctg ttacgctcag
     9901 ttcgtcgtcg taaagtcatt cagggtgatt tagaaaacca aatatcattc cgaaaagcac
     9961 tctcggattc cttaccgaat ccaacttatg ttgtaaactg gcaaggtaat gtcattagtc
    10021 ataatagtgc ttttgaacat tatttcactg cggattacta caaaaatgca atgttaccat
    10081 tagaaaacag tgattcaccc tttaaagatg ttttttctaa tgcgcatgaa gtcacagcag
    10141 aaacgaaaga aaatcgaaca atatacacac aggtatttga aattgataat ggcatcgaga
    10201 aaagatgcat taatcactgg catacattat gcaatcttcc tgcaagtgac aatgcagtat
    10261 atatttgtgg ttggcaagat attactgaaa cgcgtgatct aattaatgca ctcgaggtag
    10321 aaaaaaataa agcgataaag gctaccgtag caaaaagtca gtttctggca acgatgagtc
    10381 acgaaataag aacaccaata agctctatta tgggcttcct ggaacttctg tcgggttctg
    10441 gtcttagcaa ggagcaacgg gtggaggcga tttcacttgc ctacgccacc ggacaatcac
    10501 tcctcggctt aattggtgaa atccttgatg tcgacaaaat tgaatcgggt aactatcaac
    10561 ttcaaccaca atgggtcgat atccctactt tagtccagaa cacttgtcac tctttcggtg
    10621 cgattgctgc aagcaaatcg atcgcattaa gttgcagcag tacatttcct gatcattacc
    10681 tggttaagat cgaccctcag gcgtttaagc aggtcttatc aaatttactg agtaatgctc
    10741 tcaaatttac caccgagggg gcagtaaaaa ttacgacctc cctgggtcac attgatgaca
    10801 accacgctgt tatcaaaatg acgattatgg attctggaag tggattatcg caggaagaac
    10861 aacaacaact gtttaagcgc tacagccaaa caagtgcagg tcgtcagcaa acaggttctg
    10921 gtttaggctt aatgatctgc aaagaattaa ttaaaaatat gcagggcgat ttatcattag
    10981 aaagtcatcc aggcatagga acaacattta cgatcacaat cccggtagaa attagccagc
    11041 aagtggcgac tgtcgaggca aaagcagaac aacccatcac actacctgaa aagttgagca
    11101 tattaatcgc ggatgatcat ccgaccaaca ggctattact caaacgccag ctaaatctat
    11161 taggatatga tgttgatgaa gccactgatg gtgtgcaagc gctacacaaa gtcagtatgc
    11221 aacattatga tctgcttatt actgacgtta atatgccgaa tatggatggt tttgagttga
    11281 ctcgcaaact ccgtgagcaa aattcttcct tacccatctg ggggcttaca gccaacgcac
    11341 aggctaacga acgtgaaaaa gggttaagtt gcggcatgaa cttatgtttg ttcaaaccgt
    11401 tgaccctgga tgtactgaaa acacatttaa gtcagttaca ccaggttgcg catattgcac
    11461 ctcagtatcg ccaccttgat atcgaagccc tgaaaaataa tacggcgaat gatctacaac
    11521 tgatgcagga gattctcatg actttccagc atgaaacgca taaagatcta cccgctgcgt
    11581 ttcatgcact agaagctggc gataacagaa ctttccatca gtgtattcat cgcatccacg
    11641 gtgcggctaa catcctgaat ttgcaaaagt tgattaatat tagccatcag ttagaaataa
    11701 cacctgcttc agatgacagt aagcctgaaa ttcttcagct gctgaactct gttaaagaac
    11761 acattgcaga gctggaccag gagattgctg ttttctgtca gaaaaatgac taaattgcgg
    11821 ctcccacaat gttctaatgt gggagctatt taccagcaca tcttacattt atgatgagaa
    11881 ctcctggcga atttgttccc catgctggtc gagcgttgcc gctcccggca taacatgcgg
    11941 gtccgcgcag ccgctgattt ttatcggatt acccggcatc attattcctc cggcttcaat
    12001 caacatattt ctcgcctgag tttgtggcaa attaatggcc tcagccacac ttaataacgg
    12061 cgcgacgggc acaccaactt catgtatttt ggctaaccaa acttcagctg cctgcgtttt
    12121 taacgttcgc tcaatatatt gtttaagaat agcctggttt tgtacgcgta aaatattgct
    12181 gctaaatcgg ggatcattaa ccagttccgt aagctccagt gcctggcata acgcagaaaa
    12241 aagcttgtca ttaccacaac aaatcgtaat cggcttatcc tgagtatcga aaacatcaaa
    12301 aggtgccatg tagggatggc gatttcccag gcgttgtggt gacttccctg tcgcaatata
    12361 tgccatcaga ccatgctcca gaaaactcag cgtggcatca aacatcgcta tatcgacatg
    12421 cgcccctctc tggctctttt cgcggccata aagtgcactc actattccgc tgaataaata
    12481 gacaccgccg catagatccg caagagaggt accaacacgc actggcggag catcagggta
    12541 ccctgtttcc atcattatcc cactcattgc ctgaatgatg gtatcgtagg caggagcatc
    12601 ttttagcgga ccggtatgtc cgaaacctga cgatgaagca tatatgaggc gcgggttgat
    12661 ttcttgtaac ctttcccatg aaaaccccag tttttccatt gtacctaggc gaaaattctc
    12721 agctaataca tcagcttgtt tgagcatatt tataaatata cttttatcgt gatcattctt
    12781 taaatcaaga accacactct ctttgccatg attaataaaa ctgtaataga gtgactgtcc
    12841 atccacatag ggaccaaatg tgcgggtatc atcaccatga cctggtggct caactttaat
    12901 tacccttgcg cccatattat aaagaagttg agttccgaaa ggtccattaa ggacatgtgt
    12961 catatcgata actaataagc cttcaaacgg ccctttgctt tcattatttg tcatttgctt
    13021 atgccttgaa aaaagccgaa ggtgaaaaga cacttcggca tttatgtaaa atcaataaca
    13081 tgattaatta tgaaaccagt cgactgacat aaatccacaa gggagccgtg acgacaaagc
    13141 ccagtacgct cacagccaat gacgctgtac cagtacgggt gtagacatta aaccggctgg
    13201 caataatgat tccggagaat gccggcggta atgcgcctgc cagtaccatc atctgcaaat
    13261 gttcgctgtt caaatgacat gccataccaa cgagaagcag cgccagtggc atcagaatca
    13321 acttcaggaa ggtgttataa gcaatttcgg cactgaactc gaatttatgt gcagccagag
    13381 tcaaccccgc agcgaatacc gctacccctg agttagcctt cgcaatcaga ttaaaggttg
    13441 ggtcccatgc tgccggaatt tttaccccaa ccagcaccag gatcgttgcc agaacaggtg
    13501 cccatactac tggctccttt gccgcagaaa ttaatgcgct cagattacta ttcttcttac
    13561 catccgctcc tgaggaagga ttcagcaaat acagaccaat aggaatagta attgcgttaa
    13621 caataataga aataattgct accactaaac ctgttgatac ggaatcacca taaataggat
    13681 cgagaactgc aaaccccagg aatccaatgg taggtgaacc cgcaattaat gcacatacag
    13741 ctgcttctgc atgggtacgt ttaaaaaatt tgtagcaacc aaaccaggag aaaaagaaac
    13801 atccgacaat aaccacaagt gatacaaggg tcagacgagt gtccgcaaaa atcatttccc
    13861 tgtttgcccg ggtaatagaa acaaatagag ccgcaggaag agcgtagttt aataccagtt
    13921 tattaaatgc gcgggcttga tcttctgaaa aagtttctcg tctgccgcta aaataaccca
    13981 acagcatgat tacgataata ggcaataaat cgccaataaa aaatgttagc ataatagtat
    14041 ttccgataag ggtcccctca ccatcgcata atgagttggc aagaggagac gatgtcagta
    14101 tttaattacc agcgacttgt tttgggttaa gtttggtaat atggccactt tcagttcctg
    14161 ctgcagggtc gataaccaca ttaataatgg tcggtttgcg cgactgaata ccggtggtta
    14221 aagcatgacg aagttcatct gtcgtggtga cgttatagcc aacgccacga aacgcatcca
    14281 ttaatttgtc ataccttgca tggtgcaaca gatccgttgg tgatggtgca ccagcgccac
    14341 tgagatcaac accgtctcct ctgtagatgc cgccattatt aaaaataacg atcgtcaccg
    14401 gcaggttata tcgacaaatc gtttcaattt ccatcccact gaaaccaaaa gcactatcac
    14461 cttcaatggc gacaaccgga gaaccagaag tcacgctagc accgatggca tagcccatac
    14521 cgatgcccat gacaccccag gtaccacaat ccagacgacg acgtggttta tacatatcaa
    14581 taatatttcg tgcattatcc agggtatttg caccttcatt aactaaataa atatcctggt
    14641 tctcgcgcaa tacgtcgcgc acagcactta atgcattaaa gtaatttaat ggttgagtat
    14701 ctgtacttaa tttttcatgc attttttgtg cattttgctg cttgtggata tttaaaatat
    14761 cgcgccatac cagtggagtc gtaaatgtgt tttgtttcag ttctgccagc ataccttgca
    14821 tactggatgc aatatcacca acgactggca cagcaatggg gcggttgctg tcaatttcct
    14881 gcggttcaat atccagttga ataaactgtg tatctgccgc ccatcctttt ttaccgtgtg
    14941 ccaataacca attcagtcgt gcaccaacaa gcatgacaac gtcagcattt gccagggcaa
    15001 acgaacgcgc agctgccgca gaaagtggat gcgtatcttc aaggatccct ttcgccatag
    15061 acattggcag gaatggaatc tgagcacttt caataaattc acgaagctgt tcatcagctt
    15121 gtgaatacgc cgcgcctttg ccaaggataa ttaatggccg ttcagctttt gctaaaagcg
    15181 aaattgcgct agtgactgac ttcgggcatg gcaataatgc tggcgacgga ttttcaactt
    15241 taacaatcgt ggttaacgct tcgtcttttt ccatcgtcgc ggccaggaca tttgctggca
    15301 aatcaagata aactccgcca gggcgacccg atacagagac ccggatagcg cgtgccaatg
    15361 caatgccaag atcctgcggc tgattaacgc gaaatgctgc tttggcatac ggttttgccg
    15421 cattcatttg gtccagctct tcataatcac cttgctgtag gtcgacgatc gcgcggtcgc
    15481 tggagccgct aatcatgata atcggaaaac catttaccgt tgcgttggcc aatgcggtca
    15541 aaccattgag gaatcctggc gcagaaactg tcaggcagat ccccggtttt tgggtaagaa
    15601 aaccgcttgc cgcagcggca tagcctgccg actgctcatg acgaaaacca atataacgaa
    15661 tgccttccgc ctgggcatgg cgtgccatat ccgtcacagg aatacctaca acaccataaa
    15721 tagtgtcaat attattctgt tttaatgctt caacgatgat atgcatacca tctgtcattt
    15781 gaagttgatc tgacatgatt aataacctcg aaatttttat aaattgggag ccagaagccc
    15841 ccgttaatat cagatggcgt ggttttgctt cattgcagca atttcatcgt cgctataacc
    15901 cagctcctgc aatacagcag cggtatgttc acctaatagc ggcgcagctt taatatccgg
    15961 cgtaaaggca gagaatttca ttggacaacc aacggtcaga tattttccac gcaacggttg
    16021 ttccacttca acaacactgc cactttggcg caaagaggga tcaagtgaaa tttctttcat
    16081 acttaaaacc ggtgcacaag gaatatcaaa ctgagtcaaa taggccaccg cttcatgttt
    16141 atcaatagtg acagtgtatt tttcgatttc agcaaaaata tcgaaaatat gtggctgtcg
    16201 ggcatgggct gtactgtatg ccggatcggt aatccattct ggtttgccga tggctttaca
    16261 ggtgttttcc cagttttgct cctgaatagt gaaataaata taggcgttag gatcggtttc
    16321 ccagccttta catttcagga tccagccagg ctgaccgcca ccacctgcat taccaccgcg
    16381 gggaactgca tcaccaaatg taccattcgg atactgcggg tattcttcca gataacccaa
    16441 tttatcgaga cgctgctggt cacgtaattt cacgcggcaa aggttcaata cggcatcctg
    16501 cattgacatg gtgactcgtt gcccacgccc cgttttttcg cgatgcagca aagcagcaag
    16561 taaaccgatc agcaaatgca ttccggtgtt gctgtcaccc aacgctgcag cgcttaccag
    16621 cggcggacca tcccaaaaac ccgtagtgga tgccgcgcca cccgctgcct gagcaacgtt
    16681 ttcataggct tttacattca cataaggcga acactcatca aaccctttga tcgaaccaaa
    16741 aatcagacgt ggattgattt cttgaatatg ctcccaggtg aagcccatgt gatcaatggc
    16801 ccctggatga aagttctcga ctaagatatc agcttcgcgg atcagctttt ccattacctc
    16861 tttgccttcc gctgttttgg tatttaactc aatagaacgt ttgttactgt taagcatggt
    16921 gaagtaaagc gcatcgatat caggaatatc tcgcagttgg tgacgcgtta cgtcaccaac
    16981 gccgggacgt tcaattttaa taacgtcagc gccaaaccag gccagcattt gagtacaaga
    17041 tgggccagat tgcacaccgg tgaaatcgag aactttaatt ccttgaagtg gagttgacat
    17101 agcaatacct attagttaat taaatatgtg ttaatttagc gtttatttaa ggcataacgc
    17161 cctctataaa taatctgcaa aatatcttcg tagcggcact cgatattttt aaattcctgt
    17221 atcaagtact gaaaattatt ttatcgccac ttccaattcc tttctgcatg gtaagtagtg
    17281 aggacgaaaa gaagaatatg ataaatatta attcgtggta atcagagaga gtcgcgacat
    17341 ttgtgatgtt attcttacaa ctgtaagaag cgccggtgat ttacaagtaa gagtatgatt
    17401 acgcttttta aagcgtacca taaagcataa cataataaat gatttgtgat gacacgggat
    17461 tattgcggtt tactttgagg aaaataaaat gcgtgtttat agaaccgcga gcgccaaata
    17521 attttatgac cacaacctgt cgtattgtgg tcataaaaaa ttgtcggctg gagggagtta
    17581 aatcctttac ctcaatatac tgcctgtcag cattagttca caaacagtgc ttcgctatcg
    17641 acgatgattc catcttccgc acctttaagc gccaggtttg cctcgtagta ttgcttttta
    17701 tcaagcagtt tctgcgcatc agcaagtgca ttacgagtct gtttaagcgg catcagatat
    17761 tggttttcca ttacgccaac accggccagg cgaagctctt ccattgcgcc ctttttatcc
    17821 cctttcgcca ttttttcatt ggcaattttt atcgcggctt ctttttcggg tgttgctaca
    17881 taactctcag aaattccgac agatgcatta atgacaatat attggtcatc attgagattg
    17941 gtctttttac ctggcttagc gaactttgcc cattcggtac tgtcatctga cagcaaagcg
    18001 gaagcttcat tggtcagttc ttttgccttt tccggatcgc catgaaacag agccaggcgc
    18061 gctacctgca cgtcacgcat cgcatataaa ccttgttcag agatacgctc tgctgctgcc
    18121 gttttctggg tttgctgagt ttgctgttgt gccgctaccg gtgcattatc agcagcccat
    18181 acagttgaag atgccagaat tgctgtgacc atcgtagcca taattaaacg tttcatactt
    18241 acctcattct ttaacagttt tagaaaacgc cgccatcata ttgggtgagc atgaaatgag
    18301 aaatcagaga gcaacggtca ttttgaaagg gttatcttac aattgtagtt attatcgcaa
    18361 agcggcattt agtcttacag ttgtaggaat atgcctcctg tcgcagagaa ctattcttat
    18421 ataccacaaa gagatgtcat ttattatatt gatgtaaata ttgattaatt atatgtaaat
    18481 acagccataa catcgtatgg ttctcagtga accattttca ggataacttt ctatgaaagt
    18541 aaacttaata cttttcagct tatttttatt ggtctctatt atggcatgca atgtttttgc
    18601 attttccatt tctggtggtg taagtgagag gagctataaa gagactgaaa aaacatcagc
    18661 gatgacgacc acacactcta caaaacttca gccatcacag gcgattttgt ttaagatgag
    18721 agaagatgtg ccaccattaa acctcacaga agaaatcacg cctacttatc cgacaaaggc
    18781 gaattatctt attcatcctg tgcgatagcg tctgtcacat ggcaggtgaa caatcatgtc
    18841 tgatgctgat gtgaacacct gcccaaatga gccatcctga cccactatcg cctgccatac
    18901 cccaccataa agcgaatcat caagccacaa gccggggcaa gaaacataaa tatccccgtc
    18961 accagttcat taccgccagt taaccacatg gtgaagtaat aaatgagtgc agcgaggatc
    19021 accatgccga agaaagaaga gaccgccgat accacgttca gtacctgacc gatgtagccg
    19081 cttacgcaga caatacacag ggcgagaaac atccataaat aaatcatagc tcaacctcct
    19141 gccgcgctaa aaaacctgta acttcagtca ctttaatagt aagacaatcc ttaacctctg
    19201 gcaatttttg ctaagcatca atgaaaacag cacgttaaat gcaacgctat tgcgtgcgga
    19261 aaatttttta ctttgcacga ttaataatca gtagctgaaa gcagtcagcc agtgataaag
    19321 tagagccacc gcattcatgc tcctcgtggt tatgtcctga ctagtcttta cactctttac
    19381 aggaaccatt gtcgtacatg atggcccaac caattgagtg taccgctgtc gacatggaat
    19441 cttctgcgat acaacaattc gtatctacag aaggtaacta tgtttccaca atgcaaattt
    19501 tcccgcgagt ttctacatcc tcgctactgg ctcacatggt ttgggcttgg tgtactctgg
    19561 ctttgggtac agcttcctta tcctgttctc tgctttctcg gcacgcgtat tggcgcaatg
    19621 gcgcgaccat tcctgaaacg tcgtgaatct atcgcccgta aaaacctgga actttgtttc
    19681 ccgcagcatt ctgcggaaga acgcgagaag atgattgccg aaaactttcg ttcactcggc
    19741 atggcgctgg tagaaaccgg catggcatgg ttctggcccg acagtcgcgt acgtaaatgg
    19801 tttgatgttg aagggttgga taaccttaaa cgcgcacaaa tgcaaaatcg cggcgtaatg
    19861 gttgtcggcg tccattttat gtcgctggaa ctgggcggcc gcgtgatggg actgtgccaa
    19921 ccaatgatgg ctacctatcg tccacataat aatcagctga tggaatgggt gcagacccgt
    19981 gggcgcatgc gctctaacaa agcgatgatc ggcagaaata atctgcgcgg cattgtcggt
    20041 gcactgaaga aaggtgaagc ggtatggttt gctcccgatc aggattatgg tcgtaaaggc
    20101 agctccttcg cgccgttctt tgcggtggaa aatgtcgcca caaccaatgg cacctatgtt
    20161 ctctcccgtc tctctggcgc agccatgttg accgtaacga tggtaagaaa agcggattac
    20221 agcggatatc gtttgttcat caccccagag atggaaggct acccgacaga tgaaaatcaa
    20281 gccgctgctt atatgaacaa gattatcgag aaagagatca tgcgcgcacc ggagcagtac
    20341 ctctggatcc accgtcgctt taaaacgcgc ccggtgggag aatcgtcgtt gtacatttaa
    20401 caaactgctc ttactctttc cctcctcaaa tcggccataa taacctcatg atttttatag
    20461 cttttttctt aagttttttc cttcgctgaa tatctaatta ttaccgtcag aaattaaact
    20521 gtcgccgata tgagacactt gtaagttact aaaagtaaat aagaaaatgc ctcttgtctc
    20581 gtttcagttt taggcgtaca ttagcgccgt ctcgagcagt ttagctcaga attctggggt
    20641 gatgcccacg gtaaaataca gggcttttaa ggtcgctttg cccggtgtca actcactgta
    20701 tcaggtgtaa tgaagtcatt caggcgtaac agtaattacg cggagagatg taaagtgaaa
    20761 tatttcttta tgggcatttc ttttatggtc atcgtttggg ccggtacttt tgccctgatg
    20821 atctaaaagc aaactgtgca atgaaaaaca ggagccatca ggctcctgtt tgcatttctg
    20881 acgacggtgt ttattccgcg ttttcgtgaa tatgtttgct gctgacgggt agtaaaccgt
    20941 cggcacggaa catcgcttta atccctctga tggcctgacg aatacggtcg tggttttcaa
    21001 tcagtgcaaa gcgaacatgc gtatcaccat agtcgccaaa gccaatcccc ggcgagacac
    21061 aaaccttcgc ttcgttaagg agcttcttgg caaattccag cgatcccatg gccgcatatg
    21121 gttccgggat tttcgcccag acatacatcg aagccttcgg catttcgacc atccagcccg
    21181 cttcatgcag ccctttaacc aatacatcac gacggcgttt gtactgttcg gcaatgtcgc
    21241 gcacgcactg ttgatcgccc tcgagtgccg caatcgccgc cacctgcaac ggcgtaaaag
    21301 tgccgtaatc gtgatagctt ttgattcgcg ccagggcact gaccagcgtt ttgttgccga
    21361 ccataaagcc gatacgccag cccgccatgt tgtagctttt cgacagcgta aagaactcga
    21421 ccgccacatc gcgtgcaccc ggcacctgca taattgacgg cgctttccag ccatcgtaga
    21481 cgatatcggc ataggccagg tcatggacca ccagcacatc gtagcatttc gccagcgcca
    21541 caaccttttc gaagaactcc agttccacgc attgcgcggt tgggttcgac gggaagccga
    21601 ggatcatcat cttcggtttc ggataacttt cacgaatggc gcgctccagt tcgttgaaga
    21661 aatcgacacc ttccaccagc ggcactgagc gtacctgcgc cccggcaatc accgcgccat
    21721 aaatatggat cgggtaactt ggattcggca ccagcaccgt gtcaccatga tccagcgtcg
    21781 ccagcatcag atgcgccagg ccctctttcg aaccaatagt gacgatggct tctgattccg
    21841 ggtcgatttc aacgtcgtag cgatcctgat accagcggga aatggcgcga cgtaaccgcg
    21901 gaatgccgcg tgaagtggag taaccatgcg tgtccgggcg ctgggccaca gtacataatt
    21961 tttcgacgat atgcggcgga gtcgcaccgt ccgggttacc catgctgaaa tcgataatat
    22021 cttcgccgcg ccgacgcgca gccattttca gttcagcggt gatgttaaaa acgtagggcg
    22081 ggagacgatc aatgcgcgta aagcgacgtt cagggcgagt gtcagccata atttcctcag
    22141 attaacgtta gcgcccggac cgtccgagcg acgctgccac aatggtggct gttttgaaaa
    22201 tagcctgatt aatttctgtc tgtccagcgt tttagaacat ttttatcaat ggatttaaat
    22261 gaggaactcc acttcaaaaa caggaaattt ttctacgcta acaagaaaat caccaccaat
    22321 tttattaaca ttataatttc aataaattta ccagccagaa gtgcattatc aagagataca
    22381 ttgctaatac accatttatc ttttaatccc cttttaaatt tatggttttt ccttaaaaac
    22441 ggcctgctgg tcatcagcct tcaggttacc tatcatagag gtttaatcct tattcagagt
    22501 cacccgtgca cgaaatattc aacatgctgc tggcggtctt cgatcgggca gcgttaatgc
    22561 ttatctgcct gttctttctc atccgtatcc gcctgtttcg cgaactgttg cacaagtcgg
    22621 cgcactcccc aaaagaattg ctcgccgtta ccgccatttt ctcgctgttc gccctgttca
    22681 gcacctggtc cggcgttccc gtagaaggct cgctggtgaa cgtacgtatt atcgcggtga
    22741 tgtccggcgg gattctgttt ggcccgtggg taggcatcat taccggcgtg attgcgggta
    22801 ttcaccggta tttaattgat atcggcggcg tgacggcgat cccctgcttt atcaccagca
    22861 ttctggcggg ttgtatatca ggctggataa acctgaaaat ccccaaagca cagcgctggc
    22921 gctggatatc gccaacaaaa cgctaccgct gtttcgccat gtcaatagcg agtcattacg
    22981 caaggtctgc gaaattatcc gcgatgacat tcacgccgat gcggtggcga ttactaatac
    23041 cgatcatgtg ctggcctatg ttggcgtggg tgaacataac tatcagaatg gcgatgactt
    23101 cattagcccg actacccgtc aggcgatgaa ttacggaaaa atcatcatta aaaacaatga
    23161 tgaagcccac cgcacaccag agattcattc catgctggtg atcccattgt gggagaaagg
    23221 ggtcgtgacc ggaacgctga aaatttacta ctgccacgcg catcagatca cctcgtcatt
    23281 acaggaaatg gcggtcggtc tgtcgcaaat catctccacg caactggagg tttcacgcgc
    23341 cgagcagcta cgtgaaatgg caaataaggc agagattcgc gccctgcaaa gcaaaattaa
    23401 tccccatttt ctgtttaacg ctctgaacgc tatttcatcg tcaatccgtc tgaatccgga
    23461 taccgctcgc cagttgatct ttaatctgtc gcgttatctg cgctataaca ttgaattaaa
    23521 agacgatgag caaatcgata tcaaaaaaga gctgtatcaa attaaagact atattgccat
    23581 tgagcaggcc cgctttggcg acaagctgac ggttatctat gatattgatg aagaggtgaa
    23641 ttgctgcatt cccagcctgc tgatccagcc gttggtggag aacgccattg tccacggtat
    23701 tcagccttgc aaaggtaaag gcgttgtcac catcagcgtt gcagagtgcg gaaatcgggt
    23761 acgcattgcg gtgcgagata ccgggcacgg catcgttcca aaggtgattg agcgggtcga
    23821 agcgaatgaa atgccgggca ataaaattgg cctgctgaat gtccatcatc gcgtgaagtt
    23881 attgtatggc gaggggctgc atatccgccg cctggagccg gggacggaaa ttgcgtttta
    23941 cattcctaac caacgcaccc cagtcgcctc acaggctacg ttattgcttt gagccggagt
    24001 gatattgtga aagtcatcat tgttgaagac gaattcctgg cacaacagga actgagctgg
    24061 ctaattaaag agcacagcca gatggagatt gtcggcacct ttgacgacgg tctggacgtg
    24121 ttgaagtttt tgcagcataa ccgcgtcgac gccatttttc tggatatcaa tattccgtcg
    24181 ctggatggcg tgttgctggc gcaaaacatc agccagttcg cccataaacc gtttattgtg
    24241 ttcatcaccg cgtggaaaga acatgcggta gaagcgtttg aactggaggc gtttgactac
    24301 attctcaaac cgtatcagga gtcacgtatt accgggatgc tgcaaaaact ggaagcggcc
    24361 tggcaacaac agcagaccag cagtacgcct gccgcgacgg taacgcgtga gaatgacacc
    24421 attaatctgg tgaaagatga gcgaataatc gtcacgccaa ttaacgatat ctattacgcc
    24481 gaagcgcacg agaaaatgac ctttgtctat acgcggcgtg aatcctacgt aatgccgatg
    24541 aacattaccg aattttgcag caaactgccg ccgtcgcatt ttttccgctg ccatcgctca
    24601 ttttgtgtca atctgaacaa aatacgcgaa atcgaaccgt ggtttaataa cacctacatt
    24661 ctgcgactga aagatctgga ttttgaagtg ccggtcagcc gcagcaaagt gaaagaattt
    24721 cgccagttaa tgcatcttta atcgggaact ttcatgaaag caccaggctt gcccgccgat
    24781 caacaatttt tcgccgatct gttcagcggc ctagtgctta acccgcaact actggggcgc
    24841 gtctggtttg ccagccagcc tgcctcgtta ccggtgggca gtttatgtat tgattttccc
    24901 cgtctggata tcgtgctgcg cggcgaatac ggcaacctgc tggaagcaaa gcagcagcga
    24961 atggtggaag gagaaatgct gtttattccg gcacgcgcgg ctaatttacc gatcaacaac
    25021 aaaccggtga tgctgttaag cctggtgttc gcaccgacct ggcttgggtt atcgttttac
    25081 gatagccgca ccacgtcgtt gttgcatcct gctcgccaga cccagcttcc cagcctgcaa
    25141 cgcggtgaag gtgaagcgat gcttaccgcc ctcacccatc ttagccgttc gccgctggag
    25201 cagaatatca ttcagccact ggtgttaagt ttgctgcatc tttgccgcaa cgtggtgaat
    25261 atgccgccgg gtaattcaca accgcgcggc gattttctct atcacagcat ttgtaactgg
    25321 gtttaggata attatgccca gccgctcacc cgcgagagcg tggcgcagtt ttttaatatc
    25381 acgcccaatc atctgtcaaa actgtttgct cagcatggaa cgatgcgttt tatcgagtat
    25441 gtgcgttggg tgcgaatggc gaaggcgagg atgattttgc agaaatatca tctgtcaatt
    25501 catgaagtgg cacagcgttg cggttttccg gatagcgact atttttgtcg cgttttccgg
    25561 cgtcagttta gtctgacgcc gggagagtac agcgcccgtt ttcagggcta acgtcagaag
    25621 gttaattctg tttccagcag cgtcaggata ctttgtgcat cttgcgcggc aaacaacgac
    25681 tggcggaagt ttttattcac cagtttacgc gccagctgcg agaagacttt cacatgattc
    25741 atcccttcgt tagcacccag cgtcagcatg atcaccagtt cgacttcgcc catttctgac
    25801 tgccagtcaa ccggtttcgc cagccgggca atgctgatac tggaatgacg gatccactga
    25861 gatttggtgt gcgggatcgc tacgccaaaa ccaacgccgg tggtaacaat ctcttcccgc
    25921 tgccagacat cttcttccag ctcaaacgga tgttcagtgc gcccgttaac gccgaggttg
    25981 ccgcacagga actggatcgc ctgctcttta ttgctaaaat cctgatcaac aaagatattc
    26041 tccagcgcca gcagtgggcg aacgtcttct tccggcgtaa aggcggtgag taacgcttca
    26101 atttcctgcg cactgcggca ttcacatgcc tgacgcgcca gttcccgaca cgcctcgcta
    26161 tccagttgac gaagctggct tttcaccgcc ggaatacgcg ggctactcat actcagctca
    26221 tccaggccca gcccaagcag tagcggcaga taacggcttt caccgcccag ttcaccgcaa
    26281 atgcctaccc atttgccccg ctgatgcgca gtggtaacta tttgctgcaa catgcgcagg
    26341 aacgatggcg taatcgggtt atatagcggc gatacgcgcg ggttattacg atcgaccgca
    26401 tacagatact gggtcatatc gttggagccg atactgaaga aatcgacctc atcgcagaag
    26461 tggtcgatga tgtagcacac cgacggcact tccaccatga tcccaagcgt aatcgtctct
    26521 gcatgacgca ggccatcgcg cttaagctca acgatcgctt tttggatctc gcctttcacc
    26581 cataagatct gatcgaggct gtgaaccatc gggatcatca actgggcgtt gccgaaactg
    26641 gcggcgcgca aaatggcccg cagttgagtg cggaacaggc cagcaaattc cgggtaaata
    26701 cgtaccgcgc gatagccgag gaacgggttc tcttcctggg gaatattcag ataaggaatg
    26761 cttttatcgc cgccgatgtc catcgtgcgg aagataatcg gcttgtcgcc cgccgccagt
    26821 agcacctgct ggtaggcttc aaattgctcc tgctcgtccg gcgcgctgtc gcgatccata
    26881 tagagcattt cggtacggaa cagcccgaca ccttccgcgc cgttggcaaa cgcgcctggc
    26941 gcttccagag cggtgccgat attcgccgca atatcaatac gcttgttatc acgggaatag
    27001 gccagctgcg cggcagcctg cgcctgttgt ttttggcgtt tatccgccag cgtctgcgcg
    27061 acctgataat aaccgctcac cgcgtcattc gggttaatcg ccagcacgcc gcactgggcg
    27121 tcaagcactg caggttgccc ggcataacgg gcaatcgcat ccagcggcaa gccactcagt
    27181 accgggatcg ccgaggcacg ggccagaatc agtgtatgcg aggtgcgccc ggttttctcc
    27241 agaatcatac ccgcaagatt tttcaaatca aggctcaaaa actggcttgg ggttaaatct
    27301 tcagccacca gaatggtcgg tttttccagc accagattgt tgcgcggctt cagttccggc
    27361 caggtgatat gcagcaactg ttcgctgata tcgcgaatgt cgctgacccg ctcgcgcaga
    27421 taatcgctgg cagaagcaga tagtttggcg caaacctgct ccatattgct gatgatcgcc
    27481 gcccccagtc cctgatgctg ttctgtcatc aggcgacgga tattgcctgc aaattcatca
    27541 tcctgaatca gcgacaaatg agcgctgagg atagttttgc tttcgccgtc acgctcacgc
    27601 aattgctgat tcagttgctc ggcaagcgtt gccaggctgt gctccagccg ggtggaatct
    27661 tgcgcactgg cggggattgc ccgataactg tcgaggctgt cgctttgtaa cagggtcagc
    27721 gtacccacgc cgacgccgct tgccagcaca ttgccgtaca gtaaatccgg gttaaggcgg
    27781 cttaatgaac gcggcagcgg atgcgccgtc agttctgcct gcgtcggctg aacgctgtcg
    27841 ctgtcgataa agcgcaccag gatgtactct tccagcacgc gccgcgcctg ctcttcatcg
    27901 ctgccgctaa tgttcaggct gcaactgtca ttaaataagg tgccagttcc aatcaacgcc
    27961 agcgagcttt tagcatcagc ctttgcgttc tggcgatggt tgataaaggt gatttcgctt
    28021 tgccactggc tgcactgttc tttaagttcc cacgccggac gagcatgtag accgttaggc
    28081 aaaggacaga gaaattgaat cgttaacata accgctccgg gttaatcagg agatcatctg
    28141 aaatccgtca gttgaacaac cgtctcacgc gtaagacgtt gaataagggc agataaaagt
    28201 tgctccatct gcaaaatgtc gcggcaatcg gcaatcgatg ccgcgcaatg tccgtggcgg
    28261 gtggcaggcc ccattaccaa tgtgggtacg ccagtaccgg ttaagtgcac cgctccaccg
    28321 tccgtgccgc cgttactgaa catatccgcc tgcaacggaa cgccaatttc tgccgccacg
    28381 gtttcaatcc acgcggtaag ttttggcggc gcaatcagcg atttatcgct taacaccagc
    28441 atcggcccgt taccaatctg acgatggtta gccgcgccat aatcaaagtt tttcgcccag
    28501 caggcggtat caagcacaat ggcgacgtcc ggcgacaccg cgcgggtagc agtttgcccg
    28561 ccgcgtaatc ccacctcttc gctggaactt gccaccagcc acacttccgc aggcagttcg
    28621 gcgtcgtgca gctcacgtag taacgtcacc agcagatagc aactgaggcg atcatcaaag
    28681 gctttgccca tcacccgctg gtgagggagg acctgaaaag tggtatcgaa ggtgacgcga
    28741 tcgcctggac gaattcccgc ctgcatcact tcgtcatagg tgcgcgcgcc aatgtccacg
    28801 cgcatggcgc taacgtcatt cccctgccgg tcgccgtcaa gcaggcctgg aattttgcac
    28861 tcttcacggg tggtgatgcg caccggctgc agctggcggg cagccatgcg cacgttgcca
    28921 accggcagca cgtcaatagc gccttcgcgg gagatgctgc gcaccataaa tcccacttcg
    28981 tccatatggg cgcagatcat caccttcgga cctgtcgatt cattgaggcg gatcagcacc
    29041 gatcccagac catcaaatcg cacttctttt tgcaggcgag ccgcttcttc cagcaggatc
    29101 tgccgcactt cctgttccga ggaggcgatc gcatctgcct cgctcaacgc ttttaatagc
    29161 gataaatcca ttatgcctct cccgtgagca acactgtttt cggcatggcg tagagcactt
    29221 ctgcgccttg cggggtaacc agcacaacat cttcgatgcg cacgccccct tgccctagca
    29281 aataaatccc cggctccacg gtcagcaaca tgcctggctg tagcgtcgtg gtgtcccgcg
    29341 gtgaaaaacg cggatcttca tggacttcaa tgccgatagc gtgaccagtg ttatgaccga
    29401 aatagtcgcc aaatccagcc tcggtaatga cccggcgcgc ggcgtcgtca atctgctggc
    29461 agcgcacgcc ggggcgaatt gcagagattg ccgcgagctg tgcctgcagg acaatctgat
    29521 agacgtcaaa cagcgggtga gattcggcgc tcaccccttc gccattcacc agcaaggtgc
    29581 gcgtcatatc agcagtagcc ctgatacagc gcaccaaaat cgagagtgac aaactcgccc
    29641 gctgcaacaa tcttgtcgct ggctttgccg tgcggcagcg ccccgcgcca gccgctggcg
    29701 acaatggtgt caaaagaggc tttttctgcg ccctgctggc gcataaacca ctccagttca
    29761 gcggctatct cgcgctcgct catccccgcc tgaataaagc ggcgaatatg ctccgctccg
    29821 cgatcggcaa tcccacaggc gaggcggatt atctccacct cctctggcgt tttgatttgc
    29881 cgcagcacat ccggcgtggc gctaaccagt ttcgcattga gttcagactt ccagcggtgc
    29941 gcggtttccc aactcacctg ctggccttca aaaccgagcg tttgcaactg ctcatcggca
    30001 atgatttgat tgacgatagt ggtaagcgtg ttcgttgcgt caagcaaatg cagctggtag
    30061 ccttgcgcac gggcttccac ctcaacgaaa tagcgcgaat ctaccagaat gtgcgcactt
    30121 tcacggctaa taaccacata gcctgatccg gtggagatcc ccagatgcgg ctgtttgttc
    30181 tgccgtgagg agagaagcac cgcatccagt tgttgcacct taagccagtt gcgcagcgaa
    30241 gcgagtaatg tcatctggat tttccttctt acaggctatc gattaacaat ttgcctttgc
    30301 ggaacatcat cagacgcagg aacaccacca tcagcgcagt aatgattgcc cccagcgcga
    30361 tccccgccat ataaacgccc aagttagtga ccagcggcca tgcccagata gcggattccg
    30421 ggaaccattg cactgcgccc agccagacag ctgcggttga gccgacaatc gcgccgacca
    30481 tatatgacgg aatagcggtg atggggcttt ccagcgcaaa cggaatcgca ccctcactaa
    30541 tccccataaa ggccaggaac atcgcggtct taccctgcgg ataaagttga gcgttgaaca
    30601 ggcgtttgcc ggttaaacga cggtcaataa tggtcgccag acccagacca atcggcggaa
    30661 taacgatagc gatagaacga gcggtgaccg gtaatacgtg gtcagtggta aagctgaagg
    30721 caacgaaacc tgcggctttg ttgatcgggc caccaaggtc gattgctgtc gccgcggcga
    30781 tccccattgc gtacatcagc gcgcccttct cacctgcggc agtcagcaca gtacggatac
    30841 cgccgttgat ccagccacca aagggagtga tgacgtagta catcgccagc atgacaaaaa
    30901 tggcggaaag aatcggtagc aaaaatgtgg ttttgaacgc taacaggaaa tccggtaact
    30961 ggattttctg gttcatccat ttcacaaggt aaccggcaac gatagaaata atcagcgcac
    31021 caatgaaggt ggacggcacc ggcgaagagg tcgcccattg catagtgctg ggatcgaagt
    31081 tcagcagttg cgtcggctgg gtagacatca acccgccgat aaaaccagcc gggaacgcca
    31141 gtttgccacc gatagagttc gccacaaatg cagcaaacat cggaatggca aaaccaaaca
    31201 acacgccgcc gaaggactgc gacagccagg caaatttaag taaggagagg tcgaaaccgg
    31261 agaatttccc gctattaaga gcatccatga tgccgatctc agcaggaatt ttaagccagc
    31321 tataagcaat cagctggctg aaagcgagga tcacaccgcc cataattaag gtcggcacca
    31381 tgcgtgaaat ccctgacatc acatgctgcg ggagttcacc ccagaagctg cttttagagg
    31441 cctgcggatt cttaactgcc gctgccgcac cggatgcgcc aggcacaacg gttgcactgc
    31501 gttttttaat ggccataaca attatccctg tcgaatatta ctgttgttca gaggcaatca
    31561 tctcttcgat ttctttgatg atgcccgcag cgtttttaat tgcgtcctgc aaagtgattt
    31621 cataaacgtc gcgtgattcg aaacgttcgt tatcttccgg ggtaactgcc acggaatgga
    31681 tgatgatggt cgcttcggcg atatcctgcg ccgtcaggcg attctggatg ccgtccgcgc
    31741 cctgggtttc aattttcact tcataaccgg cttctaccgc cgcttcttcc agcacctgag
    31801 cggccataaa agtgtgagcc aggcccattg ggcaggcaca taaggcaatc agtttcttac
    31861 tcatcgttaa ttcctcgttc agtgatagtt gactagtgaa attatgaacg tgcaaaacaa
    31921 atcgccgtta ccagacaaat aatgcattta ctggagaatt aatcccgctg tacggaagtg
    31981 tgacgcaggt cggccttgtg aggggacaca aagaaaaatt gaattaactg tatgatttaa
    32041 aagattatcg ggagagttac ctcccgatat aacaggaagg atttacagaa tgtgacctaa
    32101 ggtctggcgt aaatgtgcac cggaaccgag aagacccgga tagtcatgga cgatgagata
    32161 caccggaata tcatggacat attctttaaa gcgcccttta tcttcaaatg cggcacggaa
    32221 accggaggct ttgaagaact caaggaagcg cggcacgata ccgcccgcaa taaacacgcc
    32281 gccaaatgtc ccgagattga gcgccagatt gccgccaaaa cggcccataa tgacgcaaaa
    32341 cagcgacaat gcgcggcggc aatcggtgca gctgtcagcc agcgcgcgtt cggtaatatc
    32401 ttttggcttg agattttctg gcaggcggtt gtcagctttc acaattgcgc gatacaaatt
    32461 caccagccca gggccagaaa gcacgcgctc cgccgaaaca tgaccaattt ccgcacgcaa
    32521 tatttcgagg ataatggcct cttcttcact attcggcgca aaatcaacgt gaccgccttc
    32581 gcccggcaag cttacccaac gcttatcgac atggaccaga tgcgcaaccc caagccccgt
    32641 tccggcaccg taaaccgcaa taggcttacc ttcgaccggt tctgcgccac caaactgaat
    32701 cagatgctct tttttcagca tcgggatcgc catcgataca gcggtaaaat cgttaataat
    32761 ttccagatgg ctaaaaccga gattcttttt catttcggca attgagaacg cccaggtatg
    32821 gttggtcatt gccacccagt caccggtaat tgggcaagcg atggcaatac agccgtcttt
    32881 cacctcgacc ttatgttctt caagataaac gcgaatgacc gcttcgaggc tggggtaatc
    32941 aagccctgaa taggtcttag cctgcgagat ttcaccactg gcaatatcac acagagcaag
    33001 acgtgcgttg gtgccgccca catcaccgac taatgcatac tttgtcattc ttcaactgct
    33061 ccgctaaagt caaaataatt ctttctcaca ctgtaaatac ctgggggcat aacaacaacg
    33121 ccggaaaggc aggctccctg taaatatcga tctgggtcac acaattactt tatcgtttca
    33181 gcaccaattg cagcgatgcc tttttgcaag ctgggcaaac taagtatctg accccgcata
    33241 aggaatagaa catgctccat ccgcgagcca gaaccatgtt gttattatcg ctccccgccg
    33301 tggcaattgg gattgcgtcc agtcttattc tgattgtggt gatgaaaatc gcctcggtat
    33361 tacagaattt gctctggcaa cgactgccgg gaactctggg gatagcccag gattcacccc
    33421 tctggatcat cggtgtatta acgctaacgg gtattgcggt ggggttggtt atccgtttca
    33481 gccagggtca tgccggacca gaccccgcct gtgaaccgct gatcggcgca ccggttccgc
    33541 cctctgcgct acctagactt atcgtagcat taattctcgg tcttgctggc ggcgtcagcc
    33601 tggggccgga acatccgatc atgaccgtca atatcgccct tgccgtcgcc attggcgctc
    33661 gtctgttacc gcgcgtcaac cgaatggagt ggactatttt agcctctgcc ggaaccatcg
    33721 gcgcgctgtt tggtacacct gttgcggcgg cgttgatatt ttcgcaaacc ttaaatggca
    33781 gtagtgaagt tccgctatgg gatcgtcttt ttgcgccgtt aatggcggca gcagctggtg
    33841 cacttactac cggattattt ttccatcccc atttttcact gcccattgcg cattacggac
    33901 agatggaaat gaccgatatt ctcagcggtg caattgtcgc ggcgattgcc atcgcagcag
    33961 ggatggtcgc cgtatggtgc ttaccacggt tgcacgcgat gatgaatcaa atgaaaaatc
    34021 cggtgctcgt gctgggtatt ggcggattta ttctcggaat tctgggggtt attggtggac
    34081 cagtttcgct gtttaaaggg ctggatgaga tgcagcagat ggtggcaaat caggctttca
    34141 gcaccagcga ttactttttg ctggcggtaa ttaaacttgc cgccctggtc gttgctgccg
    34201 ccagtggctt tcgcggtggg cgaatcttcc cggcagtgtt tgtcggcgtg gcattagggt
    34261 tgatgctgca tgagcacgtt cccgccgttc cagcggcaat aaccgtttcc tgcgctattc
    34321 tcggcatcgt gctggtggta acacgcgatg gctggttaag tctttttatg gcggcagtcg
    34381 ttgtacccaa taccacattg ctaccgctgc tctgtatcgt catgcttccg gcatggctgt
    34441 tattagcagg taagccgatg atgatggtca atcgtccgaa gcaacagcca ccccacgata
    34501 atgtttagca aaaaatgctc tcctttatga tttaagagtt atggcgatga tacggggctt
    34561 tacctccccg taatattgcg ttaacaggcc gctgacggct tcgatcgtga aggagaataa
    34621 caatgttcag gtcactgttt ctggcggccg ccctgatggc atttaccccg cttgcagcaa
    34681 atgcaggtga aatcacccta ctgccatcga tcaaattaca aattggcgat cgcgatcatt
    34741 acggtaatta ctgggacggt ggtcactggc gagatcgtga ttactggcat cgcaattatg
    34801 aatggcgcaa aaaccgctgg tggcgtcatg ataatggcta tcatcgtggc tgggataagc
    34861 gtaaagcata tgagcgtggc tatcgtgaag gctggcgcga tcgtgacgat catcgcggaa
    34921 aaggccgcgg acatgggcac cgccattaaa cgcgtcctcc aatggagcac aatgcctgat
    34981 gcgacgctag cgcgtcttat caggcctaca aaccggctct ttaacgcgta gggcggataa
    35041 ggcgtttacg ccgcatccgc caatagtgcc agatgcgacg ctcattcaac tacaatccca
    35101 gcgccgtccc caccaacaac cagatattca gcgccacgac cagcactaca atcacccagc
    35161 ctgtctgttt tacgcgtttg ctgttcacca gatcgcccat caacttgctg tcactggtga
    35221 aaatcagcag tggaaccagc gccagggcga taccaaaact taacagcacc tgactcataa
    35281 ccagaatccg tgtcggatct aatcccatca gaatgacaat aaatgacggc aacatggtga
    35341 ctgtacgacg cacccacagc gggatatgga agcgaatgaa tccctgcatc accacctgcc
    35401 ccgccagtgt ccccaccacc gttgaggaca gtccggcagc aaccagactt aacccaaaga
    35461 ccgttgccgc agcatggctt aacagcggtt gcagcgtcag ataagcctca tcaagatcgg
    35521 caacaccagt atgaccagaa aagtggaacg ccgccgcagc tgtagccatc atcaccagat
    35581 tgacaaaacc ggcaatcgtc atggcgatag ccacatccca tttggtggcg gaataacgtt
    35641 gttgacgcga accgccatgt aaatgctgag tgagcgaaga gtgcaaataa atcacatgcg
    35701 gcataatcgt cgcccctaac acgcctgctg ccaggaagac cgcctccgaa gtaggtaaac
    35761 tcgggatcac cattccttta cccagctgcg ccaggttagg ctgggagaaa atcaactcga
    35821 caatgtaagc cgcggcaaca aacaacagta acccgccaat cactttctcc agcggttttt
    35881 gcccgcgacg ttgcagcatt aaaatcagga aagtcgcgat ccccgtcagc accgcgccct
    35941 gcaacaacga aacaccaaga atgagtttaa aaccgatcgc cgcaccaata aattccgcca
    36001 ggtcggttgc catcgcaata atttctgcct gaacccaata gaaccacact acgggacgcg
    36061 gatagtgatc acgaatctgc tccgccagat ttttaccggt ggcaatccct agtttggcag
    36121 agaggatctg aatcagcatc gccatcaggt tggcccaaac gacaacccac agtagctgat
    36181 agccgaagct agcacccgcc tgaatattgg tcgcaaagtt accgggatcg atataaccaa
    36241 tcgccgcaat gaacgcaggt cccattaatg cgagcctcat cttacgcgcc gcccgtccgc
    36301 tgctactctc aacgcgatag ttcgtcatct tgtgcctcta aaacatagcc tttgctatgt
    36361 ttcatgctat gctaaacgag aatgattatc aaattcattt aaatggattg tggtgatttc
    36421 tctgatagac caagattatg attacgaaaa gattgatgac ttgaatgtta tggttatgtt
    36481 taatgttagc acatttacat aactttcagc ttccatacac aacatagcag aaatgtatga
    36541 cagatcacta tttttgaagc ctgtcacagg acgtcattat agtgtgtgtc agatctcgtt
    36601 ttccttaacc atgttacata gaatgtgcac ggaaatttaa cctgcctcat atttggagca
    36661 aatatggacc gcgtccttca ttttgtactg gcacttgccg ttgttgtgat tctcgcactg
    36721 ctggtaagca gcgaccgcaa aaaaattcgt atccgttatg ttattcaact gcttgttatc
    36781 gaagtgttac tggcgtggtt cttcctgaac tccgacgttg gtttaggctt cgtgaaaggc
    36841 ttctccgaaa tgttcgaaaa actgctcgga tttgccaacg aagggactaa cttcgtcttt
    36901 ggtagcatga atgatcaagg cctggcattc ttcttcctga aagtgctgtg cccaatcgtc
    36961 tttatctctg cactgatcgg tattctccag cacattcgcg tgttgccggt gattatccgc
    37021 gcaattggtt tcctgctctc caaagtcaac ggtatgggca aactggaatc ctttaacgcc
    37081 gtcagctccc tgattctggg tcagtcagaa aactttattg cctataaaga tatcctcggc
    37141 aaaatctccc gtaatcgtat gtacaccatg gctgccacgg caatgtccac cgtgtcgatg
    37201 tccatcgttg gtgcatacat gaccatgctg gaaccgaaat acgtcgttgc tgcgctggta
    37261 ctgaacatgt tcagcacctt tatcgtgctg tcgctgatca atccttaccg tgttgatgcc
    37321 agtgaagaaa acatccagat gtccaacctg cacgaaggtc agagcttctt cgaaatgctg
    37381 ggtgaataca ttctggcagg tttcaaagtt gccattatcg ttgccgcgat gctgatcggc
    37441 tttatcgccc tgatcgccgc gctgaacgca ctgtttgcca ccgttactgg ctggtttggc
    37501 tacagcatct ccttccaggg catcctgggc tacatcttct atccgattgc atgggtgatg
    37561 ggtgttcctt ccagtgaagc actgcaagtg ggcagtatca tggcgaccaa actggtttcc
    37621 aacgagttcg ttgcgatgat ggatctgcag aaaattgctt ccacgctctc tccgcgtgct
    37681 gaaggcatca tctctgtgtt cctagtttcc ttcgctaact tctcttcaat cgggattatc
    37741 gcaggtgcag ttaaaggcct gaatgaagag caaggtaacg tggtttctcg cttcggtctg
    37801 aaactggttt acggctctac cctggtgagt gtgctgtctg cgtcaatcgc agcactggtg
    37861 ctgtaaaacc atacataaaa aagccgggga tcattccccg gcttttttac gcctgttaat
    37921 cagctaatgg ctgcgggcga ccaatcaaat acccttgcag atattgcacc ccgagcttat
    37981 gcaatagcgc ctgctgctgt tgcgtctcga caaactccgc gaccacactc aatgacttcg
    38041 ctttcgccag atcggtaatt gatctcacaa tcatcgcatc cagcgtattc gtgacaatat
    38101 ctttcacaaa gacgccatca attttgatga tatcagcctg caaacgcttt aaccgttcgt
    38161 agttggcata tccggtgcca aagtcatcaa tcgcaatccg gaaaccaaac ttatgcagct
    38221 gctcgatgtt gtacatgctg ctttctgcgt tagaaaacgc ctgctcctcg gtgatctcaa
    38281 gaatgaccgc ctgcggggag atgtgataac gcttaaacag acgaataatc cgcccggcaa
    38341 tattcttttg cagcagcgtg agcggcatta aattgactga aaagcgcggt ccttttttgt
    38401 cgcaagggtg tgttgccagc cacttcaaca aggattccag cacttgcaaa tcaaaacgcg
    38461 cgctaaggtt aaactgagca ataaggggca gaaacttatc cggggtcata atgccgccgt
    38521 catatttcag tcgcgcgagg atctcatcat aaccttcacc ctctttgttg cgaattggct
    38581 gggcgtagag cagcaaatca ccctgatcta acgccgtgcg aatggtattc agcaatagca
    38641 cctgtttagt ggtctgcccg gaaaccatct cctctctgct atccagcgcc agcacatgat
    38701 gatgtgcgca ggattgctcc gccagccagc ttaactgccc caacaagggt tgcagggttt
    38761 cctgatttcc atcaaaacgc ccccaggcag caccatagcc catatccagc ccggtattgt
    38821 tccagtgaat ttgccgacta ttcaggatgt taaccatatg ctggagtcgc ccttccgttt
    38881 ccggcccgct cagcactaac agcagttcac tacccggcaa ttgatacaac ttttcgtttt
    38941 cctgcatcaa cggcagcagc gtacggcaaa ttgagcggat acagtgaacg cgcatcatta
    39001 agccgtaatg acgactcata aactcaagat tatcaatgcg caggcagcaa aaactcttgc
    39061 ccgcctcttg ctccggcgct tgttccaacg cacgaaagtt gggtaggagc gttaacggat
    39121 ctgttagcgc ctgcaaatgc cagcggcgat taagccattc actgcgatga taaatgcgca
    39181 ccatatagag caggcaaacg ctaaaggaaa tcagcaccgc gagaataaat gccagcgaat
    39241 attcggtttc caccccttgc aaaaagttct gattgtaatt cagaaggcaa agcgttgaaa
    39301 cagcccaggt gagatttaaa aacggatagc gaagattacc gaccccgagg gtaaagatga
    39361 tgaagaaaac gggtaccagg taaccggcaa taaagtcgtt ttcataaggt gtgcacaaca
    39421 gaagtagcag cacgcttaac gctgccagcc agcttaaggt aaatgcgagt gagatagtag
    39481 aaaagcatgt tgtaaatcag cactgcggtg aaaaggctta gtaaatcaac gaccgtgaaa
    39541 atggcatccg catcgccgaa aaaggtggat atctttagcg ggaaatcaaa gaagctcccc
    39601 acaagataca tgctgcattt gatgccgatc ggcgtcacca aaccaagcca gaacagacgt
    39661 tgccagacat atcggctggt caatccataa cgccaacgcg tccccagctg ccagcgtaat
    39721 attgcgcagg cacacaacac gacaaacgtc tggctgccga gcatgaccgc agtctgtaaa
    39781 aaagagagat gaaagttcca cagattggtg caaaacatcc cgcataaaac gggaacgaca
    39841 ccgcgccagc caaagataaa caagattgac agcattacgc acagtggcat ccacgccaga
    39901 aagatataac tggaatggat aatggcaagt ggcgaaataa aacgggatag ctgaataagt
    39961 accacggtga gcgtaaacgc tagtgtgaat atcttgatat tttttatcag gttatgctcc
    40021 acaaacatga gtacactact ctcactgatc ggggttttca ggcacacaaa taataagttt
    40081 attcgcatcg cgaagcaaac aggggccagc ctgttgccga tttttcatcc aatgatgctt
    40141 tcaccaggcg gcgctaaccc ggacgtagac tgtattacaa aagcggcaaa aagcagagac
    40201 aaaaaacccc cgctttgcag cgagggttgg aaatttggtg gagctaagcg ggatcgaacc
    40261 gctgacctct tgcatgccat gcaagcgctc tcccagctga gctatagccc cacgatgcgt
    40321 ttacgtacca aatttgctgg gtgcaaaatt tggtggagct aagcgggatc gaaccgctga
    40381 cctcttgcat gccatgcaag cgctctccca gctgagctat agccccgtca cgtaaagctt
    40441 gtcgagttga cgggcggcat catatgaatt ccgcccgaat gtgtcaacgg caaattgcaa
    40501 cgtgtagttt caatcgctga aaaatcaggc aaatgaacaa ttttggaaaa ccgctcgcat
    40561 tcagtagtta ttcatgtcac ggtttcctgt aaagtggtgt tataaaatga actactaata
    40621 gacccacata cattcaggga attgttatgt tcaaggagag gatgacacca gatgaacttg
    40681 ccagactgac cggctatagc cgccagacca ttaataaatg ggtacgcaag gaaggctgga
    40741 caacgtcacc aaaacctggc gtccagggtg gcaaagcccg actggttcac gtcaatgaac
    40801 aggttcgtga atatattcgc aatgctgaac gtccagaagg tcagggagaa gcgcctgccc
    40861 tttctggtga tgcgccgctt gaagtattgc tggtgacact ggcgaaagag atgacgccag
    40921 ttgaacaaaa acagtttaca tccttgcttc tgcgggaagg gattatcgga ttgttacaac
    40981 gcttagggat tcgcgatagc aaataatatg aaaagattac gcaataaaat gaccaccgaa
    41041 gaactggctg aatgcctcgg tgtggctaaa caaactgtta accgttggac cagagaaaaa
    41101 ggctggaaaa cggaaaaatt tcccggcgtg aaaggcggtc gtgccagact tattctggtc
    41161 gatacgcaag tttgcgagtt tattcagaac acgccagcct tccataacac accaatgttg
    41221 atggaagctg aagagagaat tgctgaatac gctcctggag cacgcgcccc ggcgtatcga
    41281 caaatcatca acgcgataga taatatgaca gacattgagc aggaaaaagt cgcgcaattt
    41341 ttgtcacgtg aaggaattcg caatttcctc gcccgtctcg atattgacga atcagcataa
    41401 ataaaaaacg gcaggatatt atctcctgcc gtttatcttt ttacacgcta attactgctg
    41461 attttcgcgt tcagcaataa aatccagcgc tttgttgata cgctcgatac tgcgggtctt
    41521 accaattgcg tgaacggtaa catccagtgc tggagactgc cccgcaccgg ttacggcgac
    41581 acgcagcggc ataccaactt tacccatacc cacttccagc tcatccgccg tcgcctgaat
    41641 agcgtgatga acgttttcag cggtccagtc agtaatcgcg gccagtttgt cacgaaccac
    41701 ttccagcggc tgacgcgcta ccggacgcag atgttttttc gccgcatcgg catcgaactc
    41761 agcaaaatct tcgtagaaat aacggcagct ctgtgccatc tctttcagcg tcttgcagcg
    41821 ttcgcccagc agtttcacca gatcagccag ctgcgggccg ttacgggtat cgatattttc
    41881 ctgctcaatg tgccactgta agtgagtagc aacatactcc ggcggcagcg cgttaatgta
    41941 gtgatggttc agccacagca gcttgtcggt gttgaacgca ctggcagatt tgctgacggc
    42001 attcaaagtg aagtatttga tcatctcttc acgagtgaag atttcctgat cgccgtggga
    42061 ccagcccaga cgcaccagat agttcagcag tgcttctggc aaataaccgt catcacgata
    42121 ctgcattacg ctgactgccc cgtgacgttt ggacagtttt ttaccgtcat cgccattgat
    42181 catagaaacg tgcgcgtaaa ccggcaccgg cgctttcagg gccttaagaa tgttgatctg
    42241 gcgcggcgtg ttgttgatat ggtcttcgcc acggataacg tgggtgattt ccatatccca
    42301 gtcatcgaca accacacaga agttataggt tggggaacca tcggtacggc ggataataag
    42361 atcgtccagt tcctggttgc tgaactcgat cggaccacgg atctgatcgt caaaaacaac
    42421 agaaccttcc tgcgggttag caaaacgtac aacacacggt tcatcatcag catgatgctc
    42481 atggctgtgg cggcagcgac cgtcataacg cggcttctca cctttcgcca tttgctcttc
    42541 gcgcagcgct tccaggcgct ctttagagca atagcattta taagcagtgc cctcttccag
    42601 catctgatcg atcaccgcgt tgtagcgatc aaaacgtttg gtctggtagt acggaccttc
    42661 atcccactcc aggctcagcc agttcatgcc atccataatg gcttcgatag cttccggcgt
    42721 ggaacgctca agatcggtgt cttcaatacg cagcacgaac tcaccgccgt ggttacgtgc
    42781 aaaaagccag gagtaaagag cagtacgcgc gccgccaacg tgcagatagc ctgttgggct
    42841 tggcgcgaag cgagttttga ttttcatgaa atggccttac gtttagaaag atgccgacaa
    42901 ccggcaaatc ctggaaaaat tgagtgggcg atattctatc actcccgcct gattcctcaa
    42961 tgtagttcgg gtttctacct tacgcattat tgttttcgtt gacaaattgc gcaacacggt
    43021 ctgttttgcg atcatcttgc ttaaatttac gacgaacgaa caatttcttt agaaaatgcg
    43081 ttgactcatt ttgaactctc cctataatgc gactccacac agcgggggtg attagctcag
    43141 ctgggagagc acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc
    43201 aactacttta tgtagtctcc gccgtgtagc aagaaattga gaagtgggtg attagctcag
    43261 ctgggagagc acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc
    43321 acttcgggtc gttagctcag ttggtagagc agttgacttt taatcaattg gtcgcaggtt
    43381 cgaatcctgc acgacccacc aatgtaaaaa agcgccctaa aggcgctttt ttgctatctg
    43441 cgatactcaa agattcgaac ctgcagcagg tttgagttga gcgcagcgaa acaacggagc
    43501 cgctcgcggc gacggcccga agggcgagcg aagcgagtca tcctgcacga cccaccaatg
    43561 caaaaaagcg ccctaaaggc gcttttttgc tatctgcgat ttgtaagatt gatttttctt
    43621 ctcaagttcc ttgttcttct accgatatta cctcgtacag tgacgaggac tgaacaatgg
    43681 ccactatctc ttcaacttca attccttcca tacaaacaca gagtagtaac agggcatcac
    43741 aaggtagcga tgttgcttca caaatcgcgc ggataagtca gcaaatcatc aaactcactc
    43801 aacaaattaa agagattgta gacactagcg gcagtgctga agacaagcaa aaacaggccg
    43861 aattgattca acagcaaatc accctactgg aaacacaact ggcgcagttg cagaaacagc
    43921 aggcagaaaa ggcgcaggag aaagagcagc gtttgtctct gaatgtatcc cttctaaatc
    43981 ctgttgaaaa aacgactcat atcgatatct atatctgata caacggatcc ccttcccgac
    44041 cagccattag cgcattgccc tggtctcgta ccacgtccca taacgcttca gcggcggtgg
    44101 aaagtgaacg gttcttcctg cgcaccagca tcaactggcg ttcaaccacc ggcgtaatgc
    44161 gcttaaccac cagtgggcta ccttcaggca atggtagcgc cagtgcagga agaatactga
    44221 tgccgatgcc tgcggctacc atcgggaaca gcgtcgccgg atgtccaatc tcctgcacaa
    44281 tattcgcctg aataccattg cgcgccagcg ctgcgtcaat cagcggtcgg ctgcctgacg
    44341 cgtaatcctg caacaccaat ttcgctccct gtaatgcctg ccagggtaca taatcttcca
    44401 cggccaaagc gctatcgcga tggcaaagca gaaagaaagg ctcggaaaga atcgcttcac
    44461 attgcagatc gcccactggg cctggatcga tgacgatgcc aaagtcgaca tctccctggc
    44521 gaatactttc catcacccac tgctgcggac gatcatgcag gacaaactga atatctggat
    44581 agcggcgatg gctttcagca atgcattgcg gaataagatg cgcggaaatg gtctggctgg
    44641 cagcgacccg aacttttccg ctcagttgtt gccccatacg cccggtatcg cgcaacgtgc
    44701 tgttaagttc atccagcagt cgctcaagac gcaaagccaa ctgctgccct gcatctgtaa
    44761 gcaccacttc acgcgtggtt ctgtccagca ggcgaacacc agtatgattt tccagctcct
    44821 tcacactgtg acttactgcc gactggctca agccgatacg ctctcctgca cgactaaaac
    44881 ttttctcctg cgctactgtg acgaaaacct ttaattgttt taaagaataa ttcatctgtt
    44941 ttcttcatga atggatgcaa taaatcaatt ttatttctca aactggaaga agcacaatag
    45001 aaccatcgat catctggagt ctttatgaaa ctttttcgta tcctcgatcc tttcacctta
    45061 accctgatca cggtggtgtt gctggcctct ttctttccgg ccagaggcga tttcgtcccc
    45121 ttctttgaaa atctgaccac cgcagctatt gccctgctgt tctttatgca cggcgcgaag
    45181 ttgtcgcgtg aggcgattat tgctggcggt ggtcactggc gactgcattt gtgggtaatg
    45241 tgcagcacct tcgtgctgtt tccgattctg ggtgtactgt ttgcctggtg gaaaccggta
    45301 aatgtcgacc cgatgctcta ctccggtttt ctctacttgt gcattctccc ggctaccgtg
    45361 cagtctgcaa tcgccttcac gtcaatggcg ggcggtaacg tcgcggcggc ggtttgttct
    45421 gcgtcagcat ccagcctgct ggggattttc ctttcaccat tgctggttgg tctggtgatg
    45481 aatgttcacg gtgcaagggg cagccttgag caggtcggta aaattatgct gcaactgctg
    45541 ctgccgtttg tgttggggca tctttcccgg ccgtggattg gtgactgggt gtcgcgcaat
    45601 aaaaaatgga ttgcgaaaac tgaccagacg tccattctgt tggtggttta tacagcgttc
    45661 agcgaagccg tcgttaatgg tatctggcat aaagttggct ggggatcatt gctgtttatc
    45721 gtggtggtca gctgcgttct tctggctatc gtgattgtag ttaacgtctt tatggcacgc
    45781 cgactgagct tcaataaggc agatgaaatt actatcgtct tttgtggttc gaaaaagagt
    45841 ctggcaaatg gcatcccgat ggcaaacatt ctgttcccca catcggtgat cggtatgatg
    45901 gtgctgcccc tgatgatttt ccatcagatc caattgatgg tctgtgcggt gctggcgcgt
    45961 cgatacaaac gccagaccga acagttacag gcgcagcagg aaagcagcgc cgataaagct
    46021 taaagcggac gcttcagggg ctggaccagt tgcgtcagcc cctcggtttt aatcagcagc
    46081 gtgatttgca ttagctcacc gagcttccct gccgggaact catctttgcg ggcaaaccac
    46141 agcagatact cctccggcag gtcaattaag cgacgccctt tgtatttgcc aaacggcatt
    46201 atcgtattgg ctatttcaat cagctgctct ttttccatct cagctaccca gcaaacgcag
    46261 catttccgct tcgtcgatga cttcaatgcc cagttcctgc gccttcgcca gtttagatcc
    46321 tgcagcttca cccgctatca ccagatcggt tttcttcgac acgctgcccg cgactttcgc
    46381 gcccagttcg accagtcgag ctttagcgtc atcacgcgac atctggctta agctgcccgt
    46441 aagcaccacg gttttaccag caaacgggct gtcaatctct tccgcgttga taacgatcgg
    46501 cgcaggccag tgaacacctt ccgccaacag ctcgctgatg acattgcggt tgctttcttc
    46561 ggcaaagaag ttgtgaacgt gggatgcaac gacaatgcca acatcaggca ccttttgcag
    46621 ctcttcaatc gaagcggctt ccagcgcttc cagcgtgccg aaatatgccg ccagacctgc
    46681 tgcggtggcc tcgccgactt cacggatgcc aagtgcatag aggaagcgag caaaggtggt
    46741 ttctttcgct ttttccagcg cgttaaccac gttttgtgcc aattttggcc ccatacgctc
    46801 cagtccggtc agtttgcctg cggtgagttt gaacagatct gccggagtat ggacatattc
    46861 tttttcaacc agctgatcga tgattttgtc gcccattccg tcaacatcca tcgcacggcg
    46921 ggaaacaaag tgtttcagcg actctttacg ctgcgcaccg caaatcaggc caccggtaca
    46981 gcgggcaacc gcttcacctt ccacacgctc aacgtcagaa ccacataccg gacaatgcgt
    47041 cgggaataca acctcacggg tatcttccgg gcgttcagaa agcacgacgt taaccacctg
    47101 cgggatcacg tcgccagcgc ggcgaatcac cactttatcg ccaatgcgta aaccaagacg
    47161 ttcgatttca tccgcattgt gtaaggttgc gttactcacc agcacgccag caacatggac
    47221 aggttccaga cgcgcaacag gcgtaatcgc gccagtacgc ccaacctgaa actcgacgtc
    47281 acgcacaaag gtcatctgct cctgcgccgg gaatttaaac gcaaccgccc agcgcggggc
    47341 acgcgcgaca aagccaagct gctcctgctg ttccagtgag ttgaccttaa tcaccacgcc
    47401 gtcgatatca aagcccagcg tcgggcggtc ttcttccact ttgtgataga acgccagcac
    47461 ttcttccgcc gattcacaaa gcgttacccg atcgctgacc ggcacccccc actttttaaa
    47521 ttgcagtaaa cggccaagat gagtatccgg cagctcgcca ccttccagaa caccaacgcc
    47581 atagcagaaa aaagtgagcg gtcgcttcgc tgtaatacgc ggatcaagct gacgcagtga
    47641 accagctgcc gcattacgtg ggttagcaaa aactttcccg cccgtgcgtc gcgcatcttc
    47701 gttaatcttt tcgaaccccg cctgcggcag gaacacttca ccacgcactt ccagacgcgc
    47761 cgggatattc tctccgtgca gcttcagcgg aatggcgcga atagtacgca cattagacgt
    47821 gatatcttcc ccggtggtgc catcgccacg ggtcgcggca ctgactaaaa cgccattttc
    47881 atacagaata ctgacggcaa ggccatccag cttcagctca cagcaccagg tgactttctc
    47941 gttgtttttc agacggtcct gcacacgttt gttgaaagca agaaagcttt cttcatcaaa
    48001 aacgttatcc agtgacagca ttggtacttc atggcgtatc tggctgaaag ctgccagcgg
    48061 cgcagcacct acacgttggg taggcgaatc aggcgtaatc agttctggat gtttggtttc
    48121 cagctcgcgc agttcgcgca tcagcctgtc gtattcagcg tcgggaattt ccggcgcatc
    48181 catcacatga taaagatatt catgatggca aagcgtcgtt cgcagttctg tcagttgttg
    48241 ttcgattgat tccatatcgc accatcaatg ctaaaaaccc ccgacaagcg ggggttcgaa
    48301 gaggagttaa tttgccttaa gtgtatcagg cgttggcgtc tttgacttcg cggatgatgt
    48361 cctggtactc gcgcaatttc tgcggagtca tcatacggcg ctgatcgtca agcacgacac
    48421 cgcccacttc atcggcaata tgctgcgcag attgcagcat cagcttgaag ttctgcagct
    48481 cgtcaccgta agacggtacc tgcataaaga tagtgacacc cggagtagtg aaatccttca
    48541 tttcaggatc aaaggttccc ggtttcacca tattcgccag gctgaataac gccgggccgc
    48601 tgccatccgg gctaagatga cgatggtaaa tattcatatc gccaaaaatg aagcccgctt
    48661 gttgaatgct gttaagaagc agttcaccgt ttagctcgct accgtgatgc gccgcgacgt
    48721 tcatgataat caccgcttct ttgcgcttcg gtttatccat aactggagcc ggttccgcta
    48781 caggctcagg ctgtggtgcc gctacgggtt ctgcaggctg gaaagcctgt tgtgccggtt
    48841 gcggtgctga atgcacaggc tgcggcgctg gcgcgacctg tggcgaaact ggctgctgca
    48901 acggctgttc aggctgcggc tgataggcag gctgctgcac cggctgcgct ggacgcggag
    48961 catgttgcgg cggtacctgc gcttcaggcg gctgctggac cggttgacgc ggctgcgcag
    49021 acgcataagg cggtaggtac tggtgttgcg gcgacggacg agcagcctca tgctcctgag
    49081 cgttagccgg ggcatgattc acgcggtgaa cacgaacctc accaacgccc tcatcatctt
    49141 cgacatcctc gtcataagaa tcgtcgtcac gttttgactt cattcgtttt aatggccgat
    49201 cgcggaacat agaagatcgt tctttacggc tggtccagaa accatgtacc agtaaagcga
    49261 ttatggcgat cgcgccaaca atgattaata tcagacgcaa atcctgcatc attatattct
    49321 ctgttgttct aacaccttgc caccacggca aacatttact cactaagagt atttgccgat
    49381 tacctcaagt gcaagtgcac tattaacttt cacagcacaa agatagatga aatcgtgctt
    49441 tttgctgttt tttcgaacat atcctaactg tccattgcgc aattacccgg taaaatacgc
    49501 agaattttcc tgggattggt caaaaggagc tcatcctgat tatggtttca tcattcacat
    49561 ctgccccacg cagcggtttt tactattttg cgcaaggctg gaagctcgtc tcgcaacctg
    49621 ggattcggcg tttcgttatt ttaccgctgc tggtcaatat tttgttgatg gggggcgcat
    49681 tctggtggct ctttacacag ctcgatgtct ggatcccgac tttcatgagt tacgttccgg
    49741 actggctaca atggctgagt tatctgttgt ggcctctggc tgtcatctct gtgctgttag
    49801 tgtttggcta tttcttctcc acgattgcta actggattgc cgctccgttt aacggtttat
    49861 tggctgaaca actggaagca cgattgactg gcgctacacc gccagatacc gggattttcg
    49921 gtatcatgaa agatgtgccg cgaatcatga aacgcgaatg gcaaaaattt gtctggtatc
    49981 tgccgcgcgc aattgtatta ctaattcttt acttcatccc tggtattggg caaaccgtcg
    50041 cgccggtact gtggttcctg tttagcgcct ggatgttagc catccagtat tgcgattacc
    50101 ccttcgataa ccacaaagtg ccgtttaaag agatgcgcac cgccctgcgc acacgcaaaa
    50161 tcaccaatat gcagtttggt gctttaacca gcctgtttac gatgatcccg ctgcttaatc
    50221 tgttcatcat gcccgttgcc gtttgtggcg cgacggcgat gtgggtcgat tgctatcgcg
    50281 ataaacacgc gatgtggcgg taacaatcta ccggttattt tgtaaaccgt ttgtgtgaaa
    50341 caggggtggc ttatgccgcc ccttattcca tcttgcatgt cattatttcc cttctgtata
    50401 tagatatgct aaatccttac ttccgcatat tctctgagcg ggtatgctac ctgttgtatc
    50461 ccaatttcat acagttaagg acaggccatg agtaagattt ttgaagataa ctcgctgact
    50521 atcggtcaca cgccgctggt tcgcctgaat cgcatcggta acggacgcat tctggcgaag
    50581 gtggaatctc gtaaccccag cttcagcgtt aagtgccgta tcggtgccaa catgatttgg
    50641 gatgccgaaa agcgcggcgt gctgaaacca ggcgttgaac tggttgaacc gaccagcggt
    50701 aataccggga ttgcactggc ctatgtagct gccgctcgcg gttacaaact caccctgacc
    50761 atgccagaaa ccatgagtat tgaacgccgc aagctgctga aagcgttagg tgcaaacctg
    50821 gtgctgacgg aaggtgctaa aggcatgaaa ggcgcaatcc aaaaagcaga agaaattgtc
    50881 gccagcaatc cagagaaata cctgctgctg caacaattca gcaatccggc aaaccctgaa
    50941 attcacgaaa aaaccaccgg tccggagatc tgggaagata ccgacggtca ggttgatgta
    51001 tttattgctg gcgttgggac tggcggtacg ctgactggcg tcagccgcta cattaaaggc
    51061 accaaaggca agaccgatct tatctctgtc gccgttgagc caaccgattc tccagttatc
    51121 gcccaggtgc tggcaggtga agagattaaa cctggcccgc ataaaattca gggtattggc
    51181 gctggtttta tcccggctaa cctcgatctc aagctggtcg ataaagtcat tggcatcacc
    51241 aatgaagaag ccatttctac cgcccgtcgt ctaatggaag aagaaggtat tcttgcaggt
    51301 atctcttctg gagcagctgt tgccgcagcg ttgaaactac aagaagatga aagctttacc
    51361 aacaagaata ttgtggttat tctaccatca tcgggtgagc gttatttaag caccgcattg
    51421 tttgccgatc tcttcactga gaaagaattg caacagtaat gccagcttgt taaaaatgcg
    51481 taaaaaagca cctttttagg tgcttttttg tggcctgctt caaactttcg cccctcctgg
    51541 cattgattca gcctgtcgga actggtattt aaccagacta attattttga tgcgcgaaat
    51601 taatcgttac aggaaaagcc aaagctgaat cgattttatg atttggttca attcttcctt
    51661 tagcggcata atgtttaatg acgtacgaaa cgtcagcggt caacacccgc cagcaatgga
    51721 ctgtattgcg ctcttcgtgc gtcgcgtctg ttaaaaactg gcgctaacaa tacaggctaa
    51781 agtcgaaccg ccaggctaga ctttagttcc acaacactaa acctataagt tggggaaata
    51841 caatgttcca gcaagaagtt accattaccg ctccgaacgg tctgcacacc cgccctgctg
    51901 cccagtttgt aaaagaagct aagggcttca cttctgaaat tactgtgact tccaacggca
    51961 aaagcgccag cgcgaaaagc ctgtttaaac tgcagactct gggcctgact caaggtaccg
    52021 ttgtgactat ctccgcagaa ggcgaagacg agcagaaagc ggttgaacat ctggttaaac
    52081 tgatggcgga actcgagtaa tttcccgggt tcttttaaaa atcagtcaca agtaaggtag
    52141 ggttatgatt tcaggcattt tagcatcccc gggtatcgct ttcggtaaag ctctgcttct
    52201 gaaagaagac gaaattgtca ttgaccggaa aaaaatttct gccgaccagg ttgatcagga
    52261 agttgaacgt tttctgagcg gtcgtgccaa ggcatcagcc cagctggaaa cgatcaaaac
    52321 gaaagctggt gaaacgttcg gtgaagaaaa agaagccatc tttgaagggc atattatgct
    52381 gctcgaagat gaggagctgg agcaggaaat catagccctg attaaagata aacacatgac
    52441 agctgacgca gctgctcatg aagttatcga aggtcaggct tctgccctgg aagagctgga
    52501 tgatgaatac ctgaaagaac gtgcggctga cgtacgtgat atcggtaagc gcctgctgcg
    52561 caacatcctg ggcctgaaga ttatcgacct gagcgccatt caggatgaag tcattctggt
    52621 tgccgctgac ctgacgccgt ccgaaaccgc acagctgaac ctgaagaagg tgctgggttt
    52681 catcaccgac gcgggtggcc gtacttccca cacctctatc atggcgcgtt ctctggaact
    52741 gcctgctatc gtgggtaccg gtagcgtcac ctctcaggtg aaaaatgacg actatctgat
    52801 tctggatgcc gtaaataatc aggtttacgt caatccaacc aacgaagtta ttgataaaat
    52861 gcgcgctgtt caggagcaag tggcttctga aaaagcagag cttgctaaac tgaaagatct
    52921 gccagctatt acgctggacg gtcaccaggt agaagtatgc gctaacattg gtacggttcg
    52981 tgacgttgaa ggtgcagagc gtaacggcgc tgaaggcgtt ggtctgtatc gtactgagtt
    53041 cctgttcatg gaccgcgacg cgctgcccac tgaagaagaa cagtttgctg cttacaaagc
    53101 agtggctgaa gcgtgtggct cgcaggcggt tatcgttcgt accatggaca tcggcggcga
    53161 caaagagctg ccatacatga acttcccgaa agaagagaac ccgttcctcg gctggcgcgc
    53221 tatccgtatc gcgatggatc gtaaagagat cctgcgcgat cagctccgcg ctatcctgcg
    53281 tgcctcggct ttcggtaaat tgcgcattat gttcccgatg atcatctctg ttgaagaagt
    53341 gcgtgcactg cgcaaagaga tcgaaatcta caaacaggaa ctgcgcgacg aaggtaaagc
    53401 gtttgacgag tcaattgaaa tcggcgtaat ggtggaaaca ccggctgccg caacaattgc
    53461 acgtcattta gccaaagaag ttgatttctt tagtatcggc accaatgatt taacgcagta
    53521 cactctggca gttgaccgtg gtaatgatat gatttcacac ctttaccagc caatgtcacc
    53581 gtccgtgctg aacttgatca agcaagttat tgatgcttct catgctgaag gcaaatggac
    53641 tggcatgtgt ggtgagcttg ctggcgatga acgtgctaca cttctgttgc tggggatggg
    53701 tctggacgaa ttctctatga gcgccatttc tatcccgcgc attaagaaga ttatccgtaa
    53761 cacgaacttc gaagatgcga aggtgttagc agagcaggct cttgctcaac cgacaacgga
    53821 cgagttaatg acgctggtta acaagttcat tgaagaaaaa acaatctgct aatccacgag
    53881 atgcggccca atttactgct taggagaaga tcatgggttt gttcgataaa ctgaaatctc
    53941 tggtttccga cgacaaaaag gataccggaa ctattgagat cattgctccg ctctctggcg
    54001 agatcgtcaa tatcgaagac gtgccggatg tcgtttttgc ggaaaaaatc gttggtgatg
    54061 gtattgctat caaaccaacg ggtaacaaaa tggtcgcgcc tgtagacggt accattggta
    54121 aaatctttga aaccaatcac gcgttctcta tcgaatctga tagcggcgtt gaactgttcg
    54181 tccacttcgg tatcgacacc gttgaactga aaggcgaagg cttcaagcgt attgctgaag
    54241 aaggtcagcg cgtgaaagtt ggcgatactg tcattgaatt tgatctgccg ctgctggaag
    54301 agaaagccaa gtctaccctg actccggttg ttatctccaa catggacgaa atcaaagaac
    54361 tgatcaaact gtccggtagc gtaaccgtgg gtgaaacccc ggttatccgc atcaagaagt
    54421 aattcttgcc gcagtgaaaa atggcgccca tcggcgccat tttttatgct tccgccagcg
    54481 gcggcaaaat caattcatcg ctctcatgct gctgggtgta gcgcatcact tccagtacgc
    54541 gcaaccccgc acggtgcacc gcatcgttta acgccttccc tttcagcaaa ccactgataa
    54601 gctgagcaca aaacaggtcg ccagtccctt tcaggtcggt ttttacccgt gaatgggaaa
    54661 tgacattcac gctgtcggca ctgaccacca ccacctgcat ctcctgattt tcttcattac
    54721 cggaggcgct ggtaatcacc acccatttta atgtgtctga aagcagactt tttgcggcag
    54781 caatggcact gtcgagatcg cggcaatttt taccggtcag gatttccagc tcaaagatat
    54841 tgggggtaat tccctgcgcc agcggcagta aatattgtcg atacgcctcg ggaaggtcag
    54901 gtttgacata aattccgcta tcaatatcgc caatcaccgg atcgaccatg atcaataggt
    54961 caggatggtc tttgcgtagc gcagtcagcc actcggcaag gattttgatt tgcgatgccg
    55021 ttcccatata gcccgtggtt acagcacgaa gttggcgcag cgcatcacgc tcctgaagcg
    55081 cacgcaaata gccgctaaac cattcgtccg gaatcgcacc accgtagaaa gtgtcataat
    55141 gcggcgtatt gctcagcaat accgtcggca cggcaaagac attcaagccg ttctgtttta
    55201 tagcaggcac cgcaatgctg ttgcctacgc ttccgtacac tacctgcgac tgcacggcga
    55261 cgatatccgc ctgcagcgcc ctactcttat cgttaaacaa caacaaacta ctcatttaat
    55321 tttttctcct tgccgatgat cctcatcgta ttccaaccga aactttacct gattctggca
    55381 gtcaaatcgg ctattacaaa acaaagataa ggtaattcag tgaagaaaat catttgtctg
    55441 gccattacgc tgctaatgac actccccgct tacgcgaagt taactgttca tgaagaagcc
    55501 cgcatcaacg ccatgctgga gggattagca cagaaaaagg atttgatatt tgtgcgcaac
    55561 ggtgatgaac atacctgcga tgaagcggtt tcccacctgc gtctgaagct cggcaatacc
    55621 cgtaaccgca ttgacactgc cgagcagttt attgataagg tagcttcgtc gtcatcgatt
    55681 accgggaagc cgtatatcgt gaagatcccc ggtaagagcg atgagaacgc acagcctttt
    55741 ttacatgcgt taattgcgca gacggataaa acggtgcctg cggaaggaaa ttaatccgct
    55801 ttggtaaggc atttacagga ggtaacatga aaaaatgctt tatttatcac gatgaaaaat
    55861 cgaataaatt ttggtggata gattacgaag gggatagttt agctgtcaac tatggcaagg
    55921 taggtagtat tggtaaattc catacaaaag aattcgataa tgaagaacag tgtctgaaag
    55981 aagccagtaa attgattgcc gcaaaaatga aaaaaggata tcaagaaact ccagacttta
    56041 acttcatgga tctctactat tttgatgatg aagagattgg gttacatctt aaaacgtcac
    56101 acccaaactt ccagtgccat tttactgatc cactttatat gtgttgctgg gatgaagaat
    56161 ctccttttgg tagcgatgaa ggtgctgatg ctctaaacga acttgaaaat agcctccgtt
    56221 ctggactgtg ctgatttccc tcaaatgtta attgaaacta tgtggggtat gaaatacatc
    56281 gctatggaca gtattcttga agaggatgtg cgtgcgcaat tactagccga tgaaatgagc
    56341 tctatccaga gcaatatgat tacctacgcg actgcattcg gtcagatcaa agtcatgggt
    56401 aaaatctccc ataaacttaa aaagatggga ctcaatgcac tagcgcgtca tcagcttacc
    56461 gcaaaaattc ttcaatgggg tgacggtcag gactcaccaa tacttcaaaa aatgattgat
    56521 gaccttactg cttttccaca cgaaaattaa aaactgcatt tgtcggcagc aacaactgcc
    56581 gacaaatcag attattgaca aagtgcactt tgtttatgcc ggatgcggcg taaacgcctg
    56641 atccggcata aaaatattgc taattcaata tattgcagaa acattgtagg cctgataagc
    56701 ggagcgcatc aggcagtttt gcgtttgtca tcagtctcca atgctattaa tccttaaatc
    56761 cctgccccct gactaaaatg ctcttcccca aacactccgg tggagaggta gcgatcgcca
    56821 cgatcgcaga tgatcgctac caccaccgca ccagggttag cttttgccac ccgcagtgct
    56881 ccagcaaccg cgccgcctga gctgacgcca cagaatattc cttcccgcac cgccagttcg
    56941 cgcatggtgt tttccgcatc gcgctgatga atatccagca cctcatccac cagcgcagcg
    57001 ttgaaaatcc ccggcagata ttccgcaggc cagcggcgaa tgcccggaat actgctgcct
    57061 tcttccggtt gcaggccaac aatggtcacc ggtttggatt gttcgcgcat aaagcatgaa
    57121 acgccggtga tagtgccggt cgtccccatg ctggagacaa aatgagtgat gcgcccgccg
    57181 gtttgctgcc agatttccgg cccggtggtg gtgtaatgcg cataagggtt atcgggatta
    57241 ttgaactgat cgagcagctt tccttcgcca cgattcgcca tctccagcgc cagatcgcgc
    57301 gcaccttcca tgccctgctc tttggtgaca agaatcagtt ccgcaccata agcacgcatc
    57361 gccgcacggc gttcctggct catgttgtcg ggcatcagca atttcatgcg atagcctttc
    57421 agcgcggcaa tcattgccag cgcaatgccg gtgttaccac tggtggcttc gattaagaca
    57481 tcacccggtt taatttcccc gcgcttttcc gcctcgacga tcatcgaaag tgccgcacga
    57541 tctttcaccg aacctgccgg gttattgcct tccagtttta accacacttc actgccgtta
    57601 tccggcccca ttcgctgcaa cttcaccaga ggcgtattgc ctattgtttg ttctaatgta
    57661 ctcacaatct ctatccatac gtggtgttgc ctgatgcgac gcttgcgcgt cttatcaggc
    57721 ctacaggtta caaaccttgc cattaaaaag cccggatcgc gggaagcgcc tccgggcgtt
    57781 taacattcac tcaacctatc aggcgctttg tgcgagagca agttcctcat cgcgggtttc
    57841 aatacgctcg tcgccgttat acagccgcgc atgttgcaga ccaacgaata aacgctcgcc
    57901 atgctgcggg gcatcgtcgc catgcatcac gaccgtcagc ggttcgttgt accaccccag
    57961 cggctgcacc actaattggg tgtagtgacc tttcgggctg gcttccagta cctgtaccgg
    58021 cagcggcgaa tcgagactgg tacggcggct gatatccact tcccacgggc gcaggaagag
    58081 atccaccggc ccctgatacg caggtgtgta gcccagcggc cagcgatgcg cgccaacatg
    58141 gaactgcccg ccgcgaatgg ttccctgcag gcggttcact tcgcccataa attcgagtac
    58201 aaaacgggtc gctggttcgc gccatacctg attcggcgca tcagcctgtt caatattccc
    58261 ctgactcatc accactacac gatcggctac ttcggtcgcc tcttcctggt cgtgggtcac
    58321 aaacacgctg gtgaatttca gctcttcatg cagttgacgc agccagcgac gcagctcttt
    58381 acgcacctgc gcatccagcg cgccgaacgg ttcatcaagc agcagaattt gcggttctac
    58441 agccagcgcg cgcgccagcg ccacacgctg tttctggccg ccggaaagct gcgccggata
    58501 acgatccgcc agatgcgcaa gctggaccat ttccagcaat tttgtcactt tcgctttgat
    58561 ggctgcggca ttcgggcgct cgcgacgcgg cagcaccgtc aggccaaaag tgatattgtc
    58621 gaataccgtc atatggcgga acagcgcgta atgctggaac acgaaaccga ctttacgatc
    58681 gcgcgcgtgc aggcgactca cgtcggtgcc gtggaagcga atatgcccgc tggtttgatg
    58741 ctccagcccg gcgataatcc gcagcagcgt ggttttcccg gaaccggacg gccccagcaa
    58801 cgcgaccatc tgaccagaag gaatatccag tgagatatcg ttcagcacct gggtgcgacc
    58861 aaaagacttc ttgatattgg caatctcaat gctcatgatg ctcctcctgc tgcgcgcgtt
    58921 tttcctgatt ctccaggcgc cactgcaaca tactctttaa aaacagggtg ataatcgcca
    58981 tcaacgtcaa cagcgcggcg gcggtaaagg agccgacggt gttgtagtcc tgctccaaca
    59041 attcaatctg taacggcagc gaaagtgttt cgccgcgaat cgagccggaa accaccgaca
    59101 ctgcaccaaa ctcgccaatc gcgcgggcgt tggtcagcac cacgccgtaa agcagcgccc
    59161 agcgaatatt cggcaatgtg acgcgacgga acatctgcca gccggacgcg ccaagcaaaa
    59221 tcgccgcttc gtcttcctgg ctgccctggc ttaacatcac cggcaccagc tcgcgcacca
    59281 caaacggaca cgtcacaaag atggtgacca gcaccattcc gggccaggag aacataattt
    59341 gcagattatg ttcatccagc cagccgccga gcgggccgtt ggagccgtag aacagcagat
    59401 acaccagacc ggcaaccacc ggcgatacag caaacggaat gtccagtagc gtcagcagta
    59461 actggcgtcc cgggaagtta aagcgcgtca ccagccaggc cagcagaatg ccgaacacca
    59521 ggtttaccgg tacggcaatc agcgcgatca tcaccgtcag ccagatggcg tgcagcatgt
    59581 ccggatcggc cagattctgt aaaaccggca tcagcccctt gctgaatgcc tgcacgaaga
    59641 tgtaaatcat cggcaccagc aggatgaacg ccgaaaccag catcccgatg ccaatcagaa
    59701 accatttgcc ccagttaatc gggcgcgcgt cataacgctt caattgggta acttccgcca
    59761 ttaatgacct accacacgcc gaccaaagcg actttgcaga gtgttaattg agaacagcag
    59821 cagcagagat gccgcgagga tcaccgaagc aatcgcgctc gctgccgggt aatcaaactc
    59881 ctgtaagcgc acaaaaatca tcagcgacgt cacttccgtc ttccacgcga tatttccggc
    59941 gataaaaatc accgcgccaa attcaccaag actacgggta aacgacagcg ccacgcccgc
    60001 caccagcgcc ggagaaagct ccggcagcac cactttgcag aaactctgcc agcgcgttgc
    60061 accaagcgtt tccgccgctt cttcatattc cgggcctaac tcttccagca ccggctgcac
    60121 ggtacgcacc acaaacggaa tgctggtaaa ggccatcgcc accgcaatac caagccaggt
    60181 ataggtgact ttgatatcaa acttcgccag ccattcaccg taaaaaccgt ttacggaaaa
    60241 gagcgaggcc agcgttaaac cggcgactgc cgttggcagc gcaaagggta aatccatcag
    60301 cgcatcaagc agcgtgcggc ccggaaagcg ataacgtgtt aaaatccacg ccatcagcag
    60361 accgaaaacg ccgttaaaaa tcgatgccac aaacgccgac agcagcgtca ctttgtaggc
    60421 cgcgaccacc tgcgggttgg tgatcacctc ccagtactgc gcccagctca tctcagaaag
    60481 ctgcatcacc agcgcagaga gcggcagcag caatatcagg cacacaaaca gcagactggt
    60541 gccaaggctt aaagtaaagc ccggcagcac gcgtcttgaa gaaactgcaa acattactta
    60601 cgccccgccg ctaacagctt gtctaactcg ccgccgctgg tgaagtgggt tttcatcact
    60661 tccggccatg agccaaattt gtcttccacg cggaacagtt cggtctgcgg gaatttatct
    60721 ttcagtttgt ccatgacttc cgggttattc acacggtagt aatagtcggt gatgatggtc
    60781 tgcgcctgtg ggctgtagag ccagttcagg taggcttttg cggctttttc cgtaccgttg
    60841 gcctgcacat ttttatcgac ccacgccacc gggaattccg ccagaatatt ggttttcggg
    60901 atcaccactt caaagccctg tgcttcatac tgtttacgga tgttgttcac ttccgattca
    60961 aagctaatca gcacatcgcc caagccacgc tcggcaaaag tggtggttgc gccacgaccg
    61021 ccggtatcga acacttcaac gtttttcagg aactgggtca taaactgttc ggttttggct
    61081 ttatcgccac cgtcagcttt atccgctgcg ccccatgccg ccagataggt ataacgcgcg
    61141 ttacccgacg ttttcgggtt cgggaaaatc agcttcacgt cggagcgcac caggtcgttc
    61201 cagtcgtgga tattcttcgg gttgccctta cgcaccagga agcccatggt ggagtagaac
    61261 ggcgagctat tattcggcag gcgcgactgc cagtcagccg ggatcagctt gcctttgtcg
    61321 tgcaggattt gtacgtcggt cacctggtta taagtgacaa cgtctgcttt taagccctgc
    61381 aaaatcgcca gcgcctgttt tgatgacccg gcatgagatt gttttatcgt cagtttgtcg
    61441 ccgccgttat cttttgccca ttgctgttca aacggcggat tcagggcggc aaacagctcg
    61501 cgggagacgt cataagaact gttcagcagt tccgttgcct gtacatggcc cgccagcagc
    61561 agagaagcga ccagcgcgag tgagttcttt ttcagtaagt taacggccat tgcgcaccct
    61621 tataaattta atgactttct aatagccatc atatttataa cggacgtgaa aggagtaacg
    61681 gttttatata ccgtttggtg atttggaagt taaaaaggga ataagaactt ggttcaacag
    61741 gcgtaattgt tgcaggcagt ttggacacgg acagcgcgga gaaaccggag cgtacacata
    61801 gtacgtgagg atttcgagca ctgcccgggg ccaaaatgac aaatgaaata gcctggtgaa
    61861 cttagttcaa gacgaaatcc tccccacaca tgcagggagg aaacagaggt gaatcagata
    61921 ccgacgctaa ccgtctccgg cagtgtgctg ccgccatcaa tcacattctg tgtaccggtt
    61981 aaatagctgg attcatccga tgcgaggaag gccgccagtt cgccgacttc cagcggatcg
    62041 gcgaggcgac acagcgggat tgcttttgcc atttcagtca gcaccgattc tggatcttcc
    62101 gggttcgact ggcgggcaat gctttccgcc attggcgtgc ggacgtatcc cgggcagatg
    62161 gcgttaacgc gaataccgga ttgcgcgtac tccaccgcca gcgatttagt caggccaaca
    62221 atcgccgctt tcgttaaggc atacgccgtt tcgccaggat cggccaccat atcaccagtg
    62281 actgaagaca tcatcacaat gcgaccatct ttgcgcgcaa tcatctccgg cagcaccgcc
    62341 ttcgtgacgt tccatacgcc tttaatattg atatcaatat ggaaatcgcg atcttcatcg
    62401 ctcatatcga ggaaactgcc cagacgacaa acgcctgcgt tattcaccag gatatcaatg
    62461 cgcccttctt tttccttcgc gcgtttgata gctgcggcta ccgacgccgg gtcacgcaca
    62521 tcggcgacaa ccgccgtaca gcgatgacca cgaccacaca gttcgtccgc tagcttttcg
    62581 atctcagggg agatatccag caagattagg ttcgcgccat gacgtgcaaa agttctggca
    62641 attccttcgc caattccctg caatgcgccc gtaatcagtg ctgtcttgcc cgtgagttta
    62701 cccattttaa tgctctcctt gttaatacag agcgttacac tccattccct ctgaaaatac
    62761 aggataaaca aaatattgct gcggatcagc tcactaattc aggaaaatct caatccagtc
    62821 gctgctttat gcatcatttc aggcgttgtg ttagctcact gctgcgctga atcattttca
    62881 atgacgccac atcattgatg ttgcacaaga ccaacaaaca gtaaatcggt aacagaattt
    62941 tgcgccgtgc gggtagacat cgacgagcta cgccattcgg tttcaccgga aacggtatcg
    63001 agcgtgaaat gcgccagccg ccgcagcgga gagtcagcca gagaagtaat ggcaatcacc
    63061 gttgccccct gctttcgtgc cgcttctgcg cacaagacaa tctctttttt actgccgctg
    63121 taagagatag cgatttgtac atcgcctttt ttcaacgcct gggaaacggt ggcttgtacg
    63181 tgcgtgtcgg cttcgcaggc cacgcgatag ccaattttca tcagtttgaa cgacagatcg
    63241 cgccccacca gcgccgatcc gcccaggccc gttatctgga taaacggtgc tttactgatg
    63301 acctcaatga ttttttgcag ccgcgcgtag tcgaacaacg cacaggtttg ctccagcgcc
    63361 agctcttttt cgcgattcag tttgcgggca atcacttcca gcgaatcatc actggtaatc
    63421 gaactgtgca ggtgcagcgc cgtggcattc gttttctccc ggctggcact gtattcgcca
    63481 attaacgcca tacgcaattc agtaaatcct tgcgcgccga gcttctgggc aaatttcaca
    63541 atgctcgact ggctgatacc gagctgtttc gccatctgcc gtgaagagac ggatttcagc
    63601 tcgctcactc tggcccgcag aaagtcagca attttttgct cgttcccggt aaattcactt
    63661 tccgcattgc gtattttcgt caggtacaac atcttccact ctcctgggct ggcagcaaaa
    63721 attccgctta agttcttatg acgctcttca cactctgcga gtgtagactc aatgattcct
    63781 ttaagtctgt caattcggga tatatgattc catacatctg caattatttg atcgtaaata
    63841 gtaaggtcac caccgatgca actagaaaag atgattactg aaggctcgaa caccgcctcg
    63901 gctgaaattg accgcgtatc gacgctggaa atgtgccgga ttatcaacga tgaagataaa
    63961 accgtaccgc ctgccgttga tcgcgtcctg ccggatatcg ccgcggcgat cgatgttatc
    64021 cacgcccagg tcagcggcgg cggtcgtctg atttatctcg gtgcgggaac atccggtcgt
    64081 ctggggattc tggatgccag cgaatgtccg cccacctacg gcgtgaaacc gggtctggtg
    64141 gttggtttga ttgctggagg cgaatatgcc attcagcacg cggtggaagg agctgaagat
    64201 agccgggaag gtggtgttaa cgatctgaaa aatattaatt taacggcaca ggatgtggtg
    64261 gttggcattg ctgccagcgg tcgcacgccg tatgtgattg ccggactgga atacgcacgc
    64321 cagctcggct gccgcacagt gggaatttcc tgtaatccgg ggagcgccgt ttcaaccacc
    64381 gctgagtttg ccattacacc gattgtaggt gccgaagttg ttaccggttc ttcgcggatg
    64441 aaagcaggta cagcgcagaa actggtgctc aatatgcttt ccaccgggct gatgattaaa
    64501 tccggcaaag tgttcggcaa cctgatggtc gatgtggtcg ccaccaacga aaaactgcat
    64561 gtgcgccagg tcaatatcgt taaaaacgcc accggatgta atgccgaaca agcggaagcg
    64621 gcgttaattg cctgcgagcg caactgtaaa acggccattg tgatggtgct gaaaaatctc
    64681 gatgccgcag aagctaaaaa acgcctggat caacacggcg gctttattcg tcaggtttta
    64741 gacaaggaat aacccatggc caaagagatc agcagtgaac ttctgaacac cattcttacc
    64801 cgtgtcggcg gaccgggaaa tatcgccagt tgtggtaact gtatgacgcg cctgcgtctg
    64861 ggtgtacatg acagttcact ggttgatccc aatatcaaaa cgctggaagg cgtgaagggc
    64921 gtcattttga ccagcgacca ggtgcaggtc gtttttgggc ctggtaaagc gcatcgtgcc
    64981 gcaaaagcga tgagcgagct gctgggcgat gcaccggtac aggatgccgc agaaatcgcc
    65041 gcccagaaca aacgtcagtt aaaagccaaa caaacctccg gcgtgcaaca atttctcgcc
    65101 aaattcgcca ccatcttcac gccgctgatc cccggtttta ttgccgccgg tctgctgctg
    65161 gggatagcga cgttaattgc cacggtgatg cacgttccgg cagatgctca gggcacgcta
    65221 cccgacgcgc tgaattttat gaaggtgttc agcaaaggtt tgttcacctt cctagtgatt
    65281 ctggtgggct ataacgccgc ccaggcattt ggcggcacgg gcgtcaatgg cgcaattatt
    65341 gccgcactct ttttgctcgg ttataacccc gctgcaacca ccggttacta cgccggtttt
    65401 cacgatttct ttggtctgcc catcgatccg cgcggcaata ttatcggcgt gctgattgcc
    65461 gcgtgggcct gcgcccgcat tgaaggcatg gtgcgccgct ttatgccgga cgatctcgac
    65521 atgctgctga cctcgttaat caccctgctg atcaccgcta cgcttgcgta cctgatcatt
    65581 atgccgctgg gtggctggtt attcgaaggt atgtcatggc tgtttatgca cctgaacagt
    65641 aatccgttcg gttgtgcggt tttagccggg ctgttcctga tcgccgtggt atttggcgtg
    65701 catcagggct ttattcctgt ttacctcgcg ttaatggaca gccagggatt caacagctta
    65761 tttcccatcc tttcaatggc aggcgcgggc caggtgggcg cggcgctggc actctactgg
    65821 cgggcgcaac cgcacagtgc gctacgcagt caggtacgcg gggcgattat tcccggcctg
    65881 ctgggcgttg gtgaaccgct gatttacggt gtcaccctgc cccgcatgaa gccgtttgtt
    65941 accgcctgtt taggcggcgc ggcgggcggt ttgtttatcg gattgatagc ctggtggggt
    66001 ctgccgatgg gcttaaacag cgcctttggg ccgtctggtc tggtggcgct gccgctgatg
    66061 acttccgcac aaggcatcct tccggcaatg gcggtttatg ctggcgggat tctggtggca
    66121 tgggtttgcg ggtttatttt caccacgctc tttggctgcc gtaacgtcaa tctggactga
    66181 tattatgaaa cggacaatgc tctatctttc tctgctggct gttagttgta gcgtcagcgc
    66241 cgcaaaatac cctgttctga cagaaggtag ttgatcaagt atatgcggcg ttaaagcagt
    66301 aataaaaagg ccagtcagta atgttactgc ctggccttta atcaatgaat cagaaacgct
    66361 tacagcgcca tcaacttgtc cagcgacggt gcgaaataat agccgccggt taccggtttg
    66421 gtgaaacgca acatcgcatc acgtttgcca tcggtatcgc caaacatgct cagcagttgc
    66481 tgctcaatgt tatgcagacg cgcgcagtag gcgcagaagt acagaccgtg agtgccactg
    66541 gcggtgccgt acggcaggct ctggcgaaca atcttcagcc ctttgccatc ttctttcaga
    66601 tcaacgcggg tgaggtgaga ggtttccgga cgttcgtcgc cgtcaatctc ttcgttggcc
    66661 tctttggtgc gcccgatcat catctcctga tcgtgaacgc tcatccggtt gagctgcttc
    66721 aggttgtgct cccaacgctg cacaaacaca tagctgccgc cggaatccac gccgtctttg
    66781 ataaccgcca cttcgcggcg cgtctcttca cccgccgggt tttctgtacc gtcaacaaag
    66841 ccgctcaggt cacgctcttc cacccaacgg aagccgtgga tctcttcttt cacttcaatg
    66901 cagtcaccaa aggcttccat cgccgcctga gcgacagaga agtttacgtc gtgacgcaga
    66961 gaaagaatgt ggatcaacac atcgaactgg gtcgtcggcg caaggccttt accgtagccc
    67021 ggaaaatctt tcagctcttc tgccccaacg ccgccgctca gagcgcgcca ggtgttgtta
    67081 ccaaaggcaa ccaccgcacc aagatgcgcg tccgggaatt tcgcttcaaa agttgccagt
    67141 ttgtcggcaa atgttttact ggccgcacgc agggcgtcaa cttccccttt cacgttggct
    67201 tcgatccaaa tcgccgcgcg gcaatgttct ggcaaaatgc cactctgaac ctgagacatt
    67261 gttcctcctg aaaataagaa tgccacgcaa tccgtggcat tgttgagcgc tattttacgt
    67321 gttttttcgc gacggcggtt tgttcagacg caaattaacg ccgccagata attttactga
    67381 ctttccagtt tttcagggta tcgtccgaag gcattaaccc ttcaggacca cgccattccc
    67441 cgctgaactg gtagctaata tgttgactgc cttcggcttt acattcgacg gcacgattat
    67501 catcaccatc agctttttgg caattgccaa atgctttgct gtaaaggtcg ctaaacggtg
    67561 taccgatttt aacaccggtg tcagcaggaa tatcgctatc cagcacgtca atgcgactga
    67621 tcgtgccttg atcgccgtta atcaccatcg ccacgttgtc gcctttcatc acttcaaaaa
    67681 agcgcaccac gttgccgttc gtggttttca ttccgctgcg caggcgataa tcgccatcaa
    67741 gcgcatcagc aatggcttgt tcttgcagtg gtgtggacgc cgttaattca cccacgccct
    67801 gctcgctcac tttggtggac gatccaaacc agttccacgg gttagcggca gaccagttaa
    67861 ctgacgacat cgtggaacag ccggtcagca tcaacggcat agcgcataac attaaacgca
    67921 gcgatttcat gtcacttcct ttggttattc aataacgttg cttggagtgc aaattcacca
    67981 aaaagtgccg ttattctttt tcttcattaa aacaagcgcg taaacgtcga ttggtcagca
    68041 gccagattaa cgccacaata tccgccacta ccagcgccag accgataccg ctaacggatt
    68101 caccgttcag ccacagccac ggttgccagc aaagtaaaac cacctgcgcc aacagcaaca
    68161 gaaaatagag cacatgccag gtgcgaggaa acgaagcccg ccgaccgctc aacaaaaacg
    68221 ccagcaccgc cggaataccg ggaatcagcc ccagccagaa attatcgtga tcgggataaa
    68281 acagatttag cagcgcagta ccctgctcac gcgacgcacc ggcaatgaca aacagcaccc
    68341 aggttcgcgc ctgaagcaat agcacaagcc agaagagcaa gggtaaacgc aggcgtccgt
    68401 gcgcatcata atggacagga tggaactcag tactcttcat cttcaatcaa acgcttaccc
    68461 agactcagca cgtcggcgtg ttcatatccc aggcgttcat acattccgag caccatgtcg
    68521 ttatcttccg gcacgttgat ctgaattttc gggcagccgc gagcaatcag ctttttctcc
    68581 agccggttaa gcaacgcatt ggcaattcca cgcccacgaa actctggatg cacgccaaga
    68641 taataagcag acccgcgatg cccgtcataa ccgcccatca ccgttccgac cacttcaccg
    68701 tttacctcag cgaccaaaaa caaactgacg tcatggttca tcttacgctc gatgtccatt
    68761 tccggatcgt tccacggacg cagcaagtcg caacgctccc aaagggtgat gacctcttcg
    68821 aaatcttcct ggcgaaatac gcgtatctcc atggtattcg ttaccttttt gcgggttaaa
    68881 aggctgatta tggcgtgaac ggtcgaatta gccaatatct gacgaaaatc ggttgaaaaa
    68941 gtggcataat ggggagttgt caactattga aatgaaaagt aaaacaattc tcaacagcaa
    69001 accgtcgtaa cggattacgc gatacgatat aacatctgga actttattat tacaactcag
    69061 gccgtatgag cacttttaaa ccactaaaaa cactcacttc gcgccgccag gtgctgaaag
    69121 ccggtttagc tgccctgacg ttgtcaggaa tgtcgcaagc cattgccaaa gacgaacctt
    69181 taaaaaccag caacggacac agcaagccga aagccaaaaa atctggcggc aaacgtgtcg
    69241 ttgttctcga tccaggccac ggcggaattg ataccggagc gatcggacgc aacggttcga
    69301 aagaaaaaca tgtggtgctg gcgattgcta aaaacgtccg ttccattttg cgtaatcatg
    69361 ggattgatgc gcgtttaacg cgttctggcg atacgtttat cccactttac gatcgcgttg
    69421 aaatcgccca taaacatggc gcagatctgt ttatgtcaat tcatgccgat ggctttacca
    69481 acccgaaagc tgccggtgct tcggtatttg ccctctctaa ccgtggggca agtagcgcaa
    69541 tggcgaaata cctgtctgaa cgcgaaaacc gcgccgatga agttgccggt aaaaaggcga
    69601 ctgacaagga tcacctattg cagcaagtac tgtttgatct ggtgcaaacc gatactatta
    69661 aaaacagcct gacgcttggc tcgcatattc tgaaaaagat taagccggtg cataaactgc
    69721 acagccgcaa caccgaacaa gccgcgtttg tggtgttgaa atcgccgtcg gttccttcgg
    69781 tgctggtgga aacctcgttt atcaccaacc cggaagaaga gcggctgtta ggcacggcgg
    69841 cgtttcgtca gaaaatcgcc acagcgattg ctgaaggcgt gatcagttat ttccactggt
    69901 tcgacaacca gaaagcacat tcgaaaaagc gataagttat gaaacccgac gcacaccagg
    69961 ttaaacagtt tctgctcaac cttcaggata cgatttgtca gcagctgacc gccgtcgatg
    70021 gcgcagaatt tgtcgaagat agttggcagc gcgaagctgg cggcggcggg cgcagtcggg
    70081 tgttgcgtaa tggtggtgtt ttcgaacagg cgggcgtcaa cttttctcac gtccacggtg
    70141 aggcgatgcc tgcttccgcc accgctcatc gcccggaact tgccgggcgc agtttcgagg
    70201 cgatgggcgt ttcactggta gtgcatccgc ataacccgta tgttcccacc agccacgcga
    70261 atgtgcgttt ttttattgcc gaaaaaccgg gtgcagagcc cgtttggtgg tttggcggcg
    70321 gcttcgattt aacccctttc tatggttttg aagaagacgc cattcactgg caccgcaccg
    70381 cccgtgacct gtgccagcca tttggtgaag acgtttatcc ccgttacaaa aagtggtgcg
    70441 acgattactt ctacctcaaa catcgcaacg aacagcgcgg cattggcggg ctgttctttg
    70501 atgatctgaa cacgccagat ttcgaccact gttttgcctt tatgcaggcg gtaggcaaag
    70561 gctacaccga cgcttattta ccaattgtag agcgacgtaa agcgatggcc tacggcgagc
    70621 gcgagcgcaa ttttcagctc taccgtcgcg gtcgttatgt cgagttcaat ctggtctggg
    70681 atcgcggcac gctgtttggc ctgcaaactg gcgggcgcac cgagtcgatc ctgatgtcaa
    70741 tgccgccact ggtacgctgg gagtatgatt atcagccaaa agatggcagc ccagaagcgg
    70801 cgttaagtga gtttattaag gtcagggatt gggtgtaact ccctcacccc cattcccgca
    70861 tccgctgatg caacgtcaac gacggcttct cggcaaacag ctgctggtaa tccgtggcaa
    70921 attgccccag atgccagaat ccccactgca tggcggcgtc ttttaccgtt gtgctttgcg
    70981 accacgggct tatcagttcg cggcgtacga cgttcaggcg aatgcgtttc agccacgcgt
    71041 ttgggccaat gcctaaaata gcgtgaaacg cgttttgtag cgtgcggcga ctgacatgca
    71101 gttgattaca caaatccagc accgtcaccg gttcggacat gttttccagc acatattcac
    71161 gggcgcggga aagcaaccga cggtaactct gatgactgat gctttccgcc gtcaccattg
    71221 gttgcgcttc ttccagcatc gcccccatcg ccattagcaa attatccccc agcacttttc
    71281 gcactgcagg ctgatggaga ttttccgggt tctcgcaaaa cgtcgccagc gcctgttgga
    71341 caaagcccca cagcgcggct ttatgctgct ctttcacttc cagcgccgac tggctacgca
    71401 gcatatgtaa tacccgatcc ggattgtgca aaaagttagc ctgacaggtg atgacatctt
    71461 cagacagcac tacgcccagg atcgtataat cgtccggcgt acttaattca aactcggttc
    71521 caccggggcg cgtcgctatt tctgcgcttc ccagacattg cgaaccgata aatccctgct
    71581 caccgcgcgt cgccggaatg ccaaaccaga acgagttcgg ccagaccagg cacgactgac
    71641 gcagcgccag accggtgtat tcacgaaaaa cctgaatatc atcgagtaga atttccgtaa
    71701 attcaccatg aaacttgccc ggatgcagct gatcgtaaat ctgctgctag gcggtaatcg
    71761 tcaaagcatg ttcatagaca tccgttgtct gtcgttgatg aacattatcc acttcgacct
    71821 tcggcgtgag cttcaggttt tcgggtaagg gttcatgata aagatggtgc aaattggctg
    71881 tacgggtctt tttcatgatg ttaatgccgg gtgttgtagg acacccggca cctccgacag
    71941 gttaatgggg cttgagacga taacgactac tgcgtttacg taacgtcccg gcagaaaaga
    72001 gctgttcgag cgcatttctg gctttttcca gcggccagcc aaagtgggcg gccacttctc
    72061 ctgccgtcat tccctgacat actgatgtta acagcgccaa caactcgtca gcagattccg
    72121 cgacgataac tggcgcgtcg ggttccttta cgggttgctt cggctggcgg ttaaagccag
    72181 taaccagcca ctgggtgtca tcgtctggcc gaccgatctc cttacggctg atgacacgcc
    72241 cggtacgcat cgcggcagca caacctgcgt ccagcgccgc acgacacgcc gccagatcgc
    72301 cttccaccac cagcgttaag cgaccagggt caagcacttc gtgactgagc agacgaacgt
    72361 tagctgcttt gagcatggca tccgctgcat ctatcgcggc gaccatgccg tccacttcca
    72421 gcaatcccag tgcattgatc atcggcaacc tccgttacgc acgctggatt ggattacgcg
    72481 cgatttccag cactgcatcg gtaaaggcgt tacaggctgc tttacacgcg gcctggctac
    72541 cggttaaaaa tgctgccgag tagttggttt cagacggtgg cgggacgtag gtcaccagct
    72601 gtacgtcggc agatttcaac gcggcatcaa tgccgtaggt cgcttccagc ggcggtgcca
    72661 ccagatacgc catcggatcg ccaagcgtga tcccggcggt tgatgaaaga taagaaccgg
    72721 tacgcgaaac cacatgcgcc aggaaggcgg tgttttctgc gtcattggcc cactggaaag
    72781 ctgcgccatt ttcaatatgt gcaaccatcg catccagacc ggcacgcact tccgccgggt
    72841 ttggcccacc aagcataatc agcacttcac cggcagtcgg tgacgggccg tgtgccgctc
    72901 ccgcatacag cgagcgacca tataccactt cgaccatcgc ttgtttggtc gcttcgtcag
    72961 ccgcaatgta ggttacgtcg tcagaatcgg cagaaatcag cccgaggcta cgaatatgcg
    73021 gcggcaattt tagttcacgc gcaaattcag cgttaacgga ggcaatcacc cgcatggcgg
    73081 ttaccgacgg tcgaatcaaa tctaaagctg gcatgatgcc tccttatcgg gtcatgttga
    73141 tgccggatgc tttctgctcc agcatacgtt tcgccaaatc cacaatgacg gcacagcagc
    73201 acgaaagaga agcaaaagtc agctacctgg tacactaggc agcgggtcag caaagggggg
    73261 caaatgcccc ttttttcatg tcagataatc agatcattac ctcaataaac taataatcac
    73321 atcgcggtta caaccagttt gatagtgata gctcaaaccc agacgtggcg ctggctgcaa
    73381 gcaaaataag ccgcaaaaat gcgaccccat gtttactagc cgcaaaaatg cggtcctatt
    73441 tgccagaacc ttttattatt caattggtta gtgcaagaaa aagcgtgatt actgttcaag
    73501 aagtatccag aaaggtcatg attttgacgc tgaaaaaaaa taggtgggca tttttgccca
    73561 ctcactagcc gcaaatttgc ccactcacta gccgcatttt tgcggcccaa agtaataaat
    73621 acagagaaat aataaataca gatcagcgcc aagatttttc gtcttttcgg cggaatcatg
    73681 agactagtgc ttacgtcctg ataacctcca gagcagacct taacttctgt gcagcaggac
    73741 tacgctcatt gccgctcggc taggtcaaat cctcacgata aagctgttcg gattgtgacc
    73801 ataatgttaa aggactcttt aaatcattcc ttgacgaatt aactagctgg cttaagcgga
    73861 atagcatgca ggataaaaac aaaaccagct tagcagtggg gagagcttcg cttcgctcgt
    73921 ctcctggggc caatgctcaa tgaaacgacg taaggcacgg agcgctcacc ataggtagac
    73981 acataaacat ctgagagcgc accttctatc gcgctacgcg ctcaataaaa accaacgaca
    74041 ccgccacact gcaagaccaa tggtttttat cgcgtataca gaccagacag gaaacagcag
    74101 caaagcagcg tatagccagg tgcagacagc acactcacac gacgcacagc aggcttaagt
    74161 gatcactcag gaaactttat ccccgccgcc gatcaccgcg aactatcatg accgcgccaa
    74221 attcgaaagg aatggcccat agaacgcgat gaacacacat aagcgctaca cttacttaac
    74281 gtcgccacag agcgattgag agcgcttaaa atcgatgcga tttcattaaa tttttctcac
    74341 tcgcctgaat ggtggcgatc aggactgatc gcaacattgc cccgcctcga tattgagcgc
    74401 ataatagata catacattaa gcggaaaaga gataagttta ttgtgaaaat cagggggtta
    74461 catatcatgc gcatatcaga acaacaacgg cagatcctcg actatctggc aaaaaacgaa
    74521 tcaatcaccg cacgacaagc cgcagatctc gtttatggtg gcaatgttac ccgcgtccag
    74581 gttgacgccg cccgccgctc actcatgacg atggttagta atgggctaat caggaaacaa
    74641 ggccgcgctt tcgtagctga aagacatatc aatcacgctt atagccagga cgttgaagaa
    74701 ttgcgccgcc agatcctttg ggtattcagc aaacagggtc aggagtattt cctttctgaa
    74761 cacccaaatt tcgccgccaa agagttaaca gtaagcggat tgtcgtgcgc actggattat
    74821 cttgtcggtg aaggttgcgt tacttacaag cagctatggc acgccctgaa tacgcttgta
    74881 gaagatggtt tactcattgc cacgcgtgag aacatcgaat actggataat gccgcgttca
    74941 ggtcgccgca atcatgcacg gtatatttca ggcaacagat acagaacgcc ggactttaat
    75001 ccgacacagg aagatcggga ccgtgccgaa atgatgtgca gggaacagga gaaaagaatt
    75061 atggataagt tattttcgat gcagtgaaca caagcgggcg caatgcccgt ttttattgcc
    75121 ttattccacg cctgacgaat caatttgatc acacctgaga aaatgcatag ccagaagtta
    75181 aggccgttat attgcgattg ggagcgtttt aaacggtatc aatttaatta attctgatgt
    75241 ggtgatttgc aggcaaaaaa aacccgccgg agaagcggga aatagagaca tatcgttgta
    75301 gttattaaca gatcactttg aggtcgaggg cggctaaccc tcgctaatat tatcccgcta
    75361 ttaccttgcg ggtgcaaata atcttttatt gctttgatga tttaataaat agcacttttt
    75421 gctaacaaaa accgctgtca atatatggcc aaaaatgtca acagaaaaaa taaggtttat
    75481 aatattaagt atcacatggg taatgggttc agggaatcgg aatccaccgc ctcacggaaa
    75541 ttcaaaattt gcttcttctt tcctccgtgg gcggcttctt tttcgtatcg aggtatttat
    75601 gaaagcaata acattagaac agttcgcagc aatggcgaat gtatcgacca gaacgctgag
    75661 gcgctggcta aaaaaaccat caaccggagt ccctttgcca tttaccccac gcggtactcg
    75721 ccaattattc tgggagcacg aatgcaaaga atggttgtac agaacagaac gataataatc
    75781 acgcatcaca ttttgaggta aacatcatga ccaatatcga aagattagaa cgctgtgccg
    75841 atattatgcg acgcagatgg atttatgacc ctgaagcggg tttattgttt agccgtgaaa
    75901 caaaagacgt tatcaaaggc agcatgacca ataaagggca tttggttgta acggtacatg
    75961 aaggtaaatt caaagtgaca ctcacctatc agaaagcctg ctatgtatac acatacggcg
    76021 cttatgacga gacattatat gaagtgcacc acgttaattt gaacaaacag gataaccgca
    76081 tcaagaatat tcgcctgatg ccgaaagaaa agcaccgccg gatacataaa aacgtccgca
    76141 aacttattcg gctgggccac acacaatggc ttcaaccgca attaattgaa caatcactat
    76201 aaacacagga gtaaatacca tgcattcatt agacagttat tttcagcgca cgacagcccc
    76261 taaatcagcc gcccaggaac gccgtgagga attccatgag aaagtgatgc gttctgctga
    76321 ttatattgcg gacaagttcg tggaaacggt tcgtccactt gttgatgaag tagctgataa
    76381 acttcagtct gaaatgccgg aagatatgga aggcactgca aagcggcgac tcatttgcga
    76441 attaagtcgt cgttttggcg tgtcaataag cgcttttaaa taatcgaaaa aaggggggaa
    76501 agataatgga cgcatttgag gtactggcaa ataaaggcgc tgaactggta gcacagcgtg
    76561 ataaaaccgc caacgaaggg gaacgcgcac tgctaaataa acagattaaa gcgatccgta
    76621 tggcccagtt taaattaatc agtaatgaag ttatcgaaac aattaaaccg cagatcccac
    76681 aaatcgttgc tgacgctatt aaagccgccg gactggagaa acgcattgcc gggctaaaaa
    76741 cgaacgcaga gaaaatggag atgtacaaac agagcggtgc tccgaatccc gatgagtact
    76801 tctcaccgga tgttgagttt atggcgcagg ttgaggaacg tctgggtagc tgtctcaccg
    76861 aggaacagcg tcgctatttt gatggagtgg atagttcggc aggtattgac ctgaactcgt
    76921 atttcggtcg tgaaattgag cattttgatg ccgccccgtc tatgccggag ccagaaccag
    76981 aaatggccac cgatcctgaa gctgagcaac ggaaacgcgt attcaatgcg atagggattc
    77041 ggtactgatc ggataaaaga aagccgggaa ttaccccggc tgacttaaca atgtgaccaa
    77101 tgaacaaatc ggacaagaca aacaggataa gcagtctcga aacaatgttc gggaactata
    77161 ttagcacaag tccggagaaa atcatcatgt tggcactacg catcatgcag ggcatcgcta
    77221 agaccctcgc agaacatgtc ttagacctga aacattcgcc actgagcaaa caggccatga
    77281 agcgccaaac gcttcgtctg tgggctgaat attcgcttgg aactatcaat aaaatcatcg
    77341 acatgaaatc ggggccgagc aaccagagcg cggaggaaat ggagttcatc aggcgtttaa
    77401 ttctcattcg cagagacatt catagccagt tacacagcgt gggaatcgat attaatgatg
    77461 ggacgggcga ttaattttcg gacaagtact atgcgcgcgc gaggagctga acatgaagca
    77521 agatattgca gacaggctgg agatactcga aggccagcgg gcagaagcga aacaactacg
    77581 taagcaggcc agaagagcgc acaggaacaa cgaagcagag ttactaacca aatacatcag
    77641 tttcaccaat tactgcatct acgagtgtta caaggaagat gcagaggact ggctggattc
    77701 attgccagaa cagtactgag cagaataaca ccacgtcatg ttgttctgag gtgagcatat
    77761 cacctctgtt caggtggcca aatttagtgt gccacttcag catcaccgtt gaaatcacaa
    77821 taacgatttt tttgtaaata tgctctcaca tatgtaggaa tagaccaggt tggcattgaa
    77881 acacatctac catgaccata ataaagtaat tttttgatat gtttatggtt ttatattaat
    77941 taaaatatat ctaaatttga tagttttaaa attgcaaaat ccagattgtg tttgtaatgt
    78001 ttaatctgat tgtattgagt ggcgtttatt atgaaaaata aatcaggtaa ttcttgttat
    78061 agagaagcag tcgatattca tagctcactt gagctttcat ttatattgca taaatatcat
    78121 ttgcgtgtag atgagaagaa atctcgaaat gtattcagcg ccatacgtga tgagctcatc
    78181 cttgatgggg attcacgaca aaacttagct actttctgtc aaacatggct tgatgatgaa
    78241 atccgtgagt tgcttaattt agctattgat aaaaacatga tttataaaga tgaatatcca
    78301 caaatggcgg aacctgaagg tcgctgtgtg cgtatgcttt ccgattcgtg gagtttcccc
    78361 gattcgcgac acaccttagg ttgctcaacc attggttcat ctgaagctgc tatgctcggc
    78421 cgcttggcgt taaaatggca gtggtgtaaa aaacgcgaag tacaaggtaa atcaaccgaa
    78481 cctgatttgc gatcctgtgc aaatatgctg gcataaattc tgccgttact gtgatgtgaa
    78541 attgtgtgaa attccacttg atgatcatca cctgataatg aatgctaaag aggttttgaa
    78601 gcatgtcgat gaaaacacta ttggtgtcgt tacgacttta ggtattactt atttttgtaa
    78661 agcagattgc gttagtgttt ttaagataag tgttaaagtt aatattagtt tttatattta
    78721 catcgtaatt atatgtttct attttaaaaa tgttcgagag tataaaaatg tctaatgcta
    78781 atatcgcacc caaaagtaag aaaatgacag tggggacact cgctatcatg aatataactg
    78841 ctgtagtaag tctacgattt ataccttctg aagcagaata cggcttaggt gccatttttt
    78901 actatacctt tgctgcaatt atgtttttag tacctgttgc ctttgttgcg gctgagctag
    78961 ctacaacata tccacaaaaa gggggggcat ttcgatggat aggagaagca tttggggctc
    79021 gctggggttt tgtaggaatg cttatgacct ggctacaagt catcccctac tttccaacag
    79081 tactgacatt tggtgcagtc tcggttgcat ttattgatgt tgatatgggg gtggcagaat
    79141 caattgccag taataaatgg tatattttat ttttcgtact atttgtttac tggggagcta
    79201 tttgtgttgc attacgtggc gttggtattt tctcgcaggt atctaagtgg tgcggtatta
    79261 tagggacaat tattccagct attatccttg ttattcttgg tttctcctat ctgtggttca
    79321 gcggtaaacc agcggtgatc ccattaggtt ggggtgatct tttccctaat atagctaact
    79381 tccagaatat tgttcttgcc gccagcatat ttcttgctta tgcagggatg gaaatgaatg
    79441 cggtccatgt taacgattta gataatccaa ctaaaaaata cccaattgca attaccatcg
    79501 cttctatagg tactattgca atattcctgc tcagtacttt aggggtcgcc tttattattc
    79561 ctacagataa aattagtcta actcaaagtt tgttggttgc ctacgatatg ctgttcgaat
    79621 gggctggagt gccatggctt ggctctgtga tggcatttat gctagcaatt ggcgtactgg
    79681 gtggggtggt tacttggatt gcagggccta atacaggtgt attagcgatc gcgaaagctg
    79741 gttatttacc taagtttttc caaaaaacca atcgtcatgg aatggggcac catctaatgt
    79801 ttgtacaagg tataattgtc tcagttcttt ctgttacttt tgtgataatg ccttcagttc
    79861 aagcagcttt tcagatttta tcacagttaa cagttatgct ctatttagtt atgtatatgc
    79921 tcatgttcgc tagtgctatc tatctacgac attctcaacc tgatacaaaa cgcccatatc
    79981 gagcccctgc tttatttttc tgggcaacac ttggattctt agggtctttt ctggcttttg
    80041 tattaagttt tattccgccc gagcaaatcc caactggtag tccaaccagc tatgttcttt
    80101 atttgttagt attagttgcc ctatttttat taattccaat attaatttac gccttccgaa
    80161 aatctgactg gaaagatcca aactctgatt tcgaaccatt ttcttgggaa aaaagaacta
    80221 gtaattaaat tttccataag ctacaaaaga tgaggttaaa tatgtttagt gctgaatctc
    80281 caattggtgt aatagactca ggtgtcggtg gattgacatc ggtaaaggaa ataattaatt
    80341 tgcttcctgg tgaagatatt atctattgtg gagataatat gaatgcacct tatggcaacc
    80401 gttctgccga tgatatcatc agtttgacta aaaaaatgct gactttttta cagtcaagga
    80461 atgtcaaact agttgctgtc gcatgtaata ctatatcctc aactttggaa tccgaagaat
    80521 acagcggata tgctaagagc tttccgtttc ctatcttatc catcattgaa cctgcagtag
    80581 aagatgtgat tcgccagcaa tataaaaatg ttggtattat cgctaccgag tttactatta
    80641 aaacggggtg tcacaaagag ctaataaaaa aattgaattc aacaataaat gtatttggag
    80701 aaccaagcaa aaatcttgca atgttaattg aggaaggtaa tcttaatgct cctgcaatct
    80761 tgaatgacat caaaaaacat gttaaccatt taatttccct ccatccagta aatgaaatta
    80821 ttctcggctg tactcactat ccaattgttc agaatttttt tgaagcagtt gcaccggata
    80881 taaaatttat taatcctgca catgatcagg ctatctctat taaaaatcac ttgggacagc
    80941 taaatttatt aaactcctca aatataggaa ctttgcaaat aaatactagt ggaagtatgg
    81001 agatctacaa aacggtacta ggggaattga gcattacgaa acctcataca ttcagtatca
    81061 ggcagtttta aatttatccg gtacaccgta aaatcccgtc cttctgaact gcaccccaaa
    81121 agttggacac caactgagta aggtgcattt tatggacaaa cccagttaca tactgggtag
    81181 tgcatccaat tagtagaaca tgtgtttttc gataaacgct ccgatcactt tttcgtggat
    81241 ctcaactgag cgtgagaagc agattgtttt acgacccaac cgcttaatgc gggtgcgtag
    81301 cgtcagatta ttacgctcaa tgcgttgggt gaatattttg ccggtcagat gcttattctt
    81361 cggcacctcc cggccatagc tgccccagtc atcgctggtc agcatgccga tgttgaaggg
    81421 tgtaagcagt gccagtagct cccggcacgt ttgatcggtt cggggaccaa aagtgtaggc
    81481 cagtacaccg cctgttttgg tgttgtacgc gtacccgagc caatgttgcc gggctttact
    81541 gccaacgtag ctccattgct catcaagctc gcagataaac gccacatcag catgggcaac
    81601 aggcgaagaa gttattcgct ttggcgcgag ttttttaaag tccggatgac ggtgttacta
    81661 ccaattttca gtgtcctggc ggtatcgcga accccggcac cattaaaggc catttcagtt
    81721 atcagctctt tcatacccgg cttacgtgct tgataagtat aagtgaactg aaacacacgg
    81781 tggcagtcac ggcagcgaag tctgtcacgg cctttagggt tctgaccatg gcggtaaacc
    81841 tgagctgact gacaacgggg acaatgaata ttaacgctgg tcatgagata acctcaaaag
    81901 cccgtattat acatcagatt caactaatta gaggcatcac ccatccagca ttataaaaca
    81961 tactaacaat aactaaatta taataatcac aacttacaat caaataataa atgcataaga
    82021 ttaacaatga ttttttcggg ttatatttat ctaaaaaagc agaacattat gtaacttatt
    82081 attttctaag cgaaaattta ttgtgcggtc agactaacaa acttgcgtac cacccgaaga
    82141 acatgcgaag atttaagtat tctttctttt ttggttttta tttttcattt tctcttttct
    82201 tctctttccg gttctgttct ggttttggtt ttttcctttt tatttttatt ctttcttcag
    82261 ggttatgcta aaacaccaaa acatttttca ccacaaaagc atccaatcat cttgccagac
    82321 caatcacctt aaccggatac actgccggat ataatcccac taacacccag acaccaagca
    82381 ccatacctga taactaccgt attcaggaag gaaatgcgag tgatttcacc gtcaccggca
    82441 caaccgatcc gccaaaaaga ggcgtaccaa tgtcgatata gtcccccgcg cggacaatca
    82501 cttcgtcaat gactgccaac gggagttgtt gtagctgtgg gcgcaacaac atgcccagag
    82561 ctttaccaaa gtcctgcccg gccaccacca gcaggggatg cggattcgga aaacgcgcga
    82621 cgaaatcgac cagcgcgttg atgaccgtca gaaccgcggc gtaacgcaca ggcagcgagg
    82681 cgggaagcgc cagcacgtac acgtcagttt tgggatcaag atccagctga agcagcgcct
    82741 gttgccaggc actcaccaga tccgtttcat caatcgggat cgccaccggc aaattgcgca
    82801 gcggcagttg tacgccctcc agccagattg tgctgccaga gagcgaaagg gtatgtgcac
    82861 ccgcgccaat caccgtggag cgtacggttt gcgccggaaa ctgcacattc atctcacgca
    82921 ggcgcggatg gtcatgcagc gccgttgcca gaagtgggcc aatatcggca aaacagaacg
    82981 ggttggcggg ctggtggcga taacattcgc ccacgcctcc agaaagcgta atgatttcgg
    83041 gcgtaacacc tgcgggcagc aaaccggttt gcatcaatgc ctgcgcgagc ggcgaaagcg
    83101 ttccgtcaat cacttcgaca atcagcgccg ccatccgccg ggtaacctgc accagctgcg
    83161 cgccggtcag cgaacgggcg tcagtgcctg caccgaagca ctcatccaca atcatctgcc
    83221 ccggtttatg agcgtaaacc acgcgcccct ggctgtcggt ttccagcagg cgaccaccga
    83281 cgttgagaca ggcagtgccg ctgatttttc cggcatcgaa cagggcgtag ttcgcggtgc
    83341 caccgccgat atcgatattc agtacccgac acagccgttg ttcagaaagg gtttgcgccc
    83401 cggctccgtg accggcgatc acggattcga ggtgcggccc ggcgctggcg acgacaaaat
    83461 cgcccagcga ctgtttttcc ttcatcactt tttccatcag ctgtgcgacc aggctttcgg
    83521 taaactggcc ttccggcagc tgggcaatga tggtttcgcg gatgcgctgg ctttcggttt
    83581 tgtcggctgg cacgctggcg ggcacggacg ccgttaccgg gatcgattca tcacattcgg
    83641 tgatggtaaa acccagtaga tccgccactt cacgggcttc cggggtaata atgctggcgc
    83701 gcagaaccac ggacattgcc tgttcgccgc gcgcgtgtgc ttcacgaata tcattcgctg
    83761 tgatgagttt tttcacctgc ttcctcctgt ttggtgagag atgccagata atccaccagt
    83821 tgctgcacgc tttgcgggtc gtggctgttt agctcgaaaa tcggctcttc gaaacccgtc
    83881 tcaagcaata actttcgcgt tgcggcgacg tcagcatctg gcatgtccgt tttactgatg
    83941 acggcgattt gccgtttgct gacgccaata tccagcaacc cggcaggcaa ccgactttcc
    84001 gggtcatttg cgccatggac atagatcagc atgtctacat cctgtagcgt ggtaattaag
    84061 gcgtgatacc agcggggatg gctaaaatat tcacccggcg tatcaatatc gcccttatca
    84121 ttaaattcca cggccagtgt ttttctggcg agggtataat tcccctgcaa cgcattaaat
    84181 aacgttgttt ttcctgcccc gacgctaccc acaaacgcaa ttcgtttcat gaatgcctcg
    84241 tattaacttt tggtcatttc acaaagcgta taatttaata agcgacctaa accgctgacg
    84301 gtttgtgata acgcttcttc tactgcgccc accgagccat aaatcaccaa tgcaccgctg
    84361 aagcggtcga gaaaaccgat atgcacatca gcggctttca gcgctaaatc accggcgatc
    84421 atcgccgttt cgccgggagt tagcgtcata atgccaattg cgcccgcatc cggaacgccg
    84481 attttcttcg ccagttcctc accgggatgc gcgatgagat gcgccagtgt gacctgtttg
    84541 ccgggcacaa attcctgaat gatgcgttct ttatccatga gtcgccgcct gtgaaaaact
    84601 ttcgtgtatc gtgacaattt ttcgtcagag taaataacaa aattcggcaa gcgcgtttaa
    84661 aaggtgaggt agatcaataa gaatggagag atatttactg ttatgaataa actttcatat
    84721 gcgacgcatg aatataaata aaataatatc gagatatttt atatactgat ttttttatta
    84781 ttcgcaaact cagtaataag ctttgctaat atatttcagg aaaaccgctt accctgaata
    84841 ttcagggtaa gcgtgagagt taagaatgaa ttacagcggt tgggtttgcg cttcaaccac
    84901 ggccagcgcc accatattga cgatacgacg caccgatgcg atcggcgtta acacgtgaac
    84961 cggtttcgcc acgcccatca gcaccggacc gacagtcaca ccttccgagc tggaaacgcg
    85021 cagtaagttg taactaatgc gggcagcttc catgttcggc atcaccagaa tattggcaga
    85081 acctttcaaa gggctgtccg gcatacggtc gttgcgaatc gcttccacca gcgctgcatc
    85141 gccgtgcatt tcaccatcaa tcatcagatc tggcgcacga tccctgacca gttccagcgc
    85201 ctgacgcatt ttgctcgacg acgggcagtc agaagaacca aagttggagt gcgacaacag
    85261 agcaacgcgc ggctcaatac caaaacgacg gacagtttct gccgccatca aggtgatctc
    85321 cgccagctct tctgcgtctg gttcgtcatt gacgtaggta tcggcaataa aggtgttacc
    85381 actcggcagc agcagcgcat tcattgcacc tgcggtgtga acgccatcgc gataaccaaa
    85441 gacatttttc accacactaa aatgttcatg ataatcgccc accgtaccgc agatcattgc
    85501 atcggcttcc ccacgctgaa ccatgatcgc gccgatcact gtcgggttac tgatcagcgc
    85561 acgctgcgcc tgttcctgag tgacaccgcg acgcttcatg atctggaagt attcggtcca
    85621 gtactcttta aagcgcggat cggattcgtt attgacgatt tcaaaatcaa cgcccgcttt
    85681 gatctgcaag cccagtttct gaatgcgcat ttcgatcacg ttcggacgac cgataaggat
    85741 cggtttcgcc agtcccagcg ttaccagttc ctgagtcgca tgcagaacgc gcgcctcttc
    85801 cccttccggc agaactacgc gcttcggcgc tttgcgagcc tgggagaaaa tcggcttcat
    85861 aaacaggttg gttttgtaaa cgaactcagt cagcttgtcg atgtagacgt cgaaatcagc
    85921 aatcggacgc gtcgccacgc ccgactccat cgccgctttt gcgaccgcag gagcgatctt
    85981 aacgatcaag cgcggatcaa acggttttgg aatgatgtat tccggaccaa agctcagatc
    86041 ctgaacgcca tacgctgaag ccaccacttc actctgttct gcatgggcca gttctgcaat
    86101 cgcgcgtacc gccgccagtt tcatctcttc gttgatggcg gttgcgccaa cgtccagcgc
    86161 gccacggaag atgaacggga agcaaagaac gttgttcacc tggttcgggt agtcggaacg
    86221 gccggtacag atgatggcat ccggacgcac ttctttcgcc agcggcggca gaatttccgg
    86281 ttccgggttc gccagcgcca ggatcattgg tgcccgagcc attttcttca ccatttcctg
    86341 ggtcagcact ttcgggccgg aacagcccag gaaaatatcc gcaccttcaa tcacatcatc
    86401 gagggtacgt ttgccgtcat ccaccaccgc atatgcggct ttggtttccg ccatatttgg
    86461 ctcacggccc tgatagataa cgccttttga atcgcaaacc acgatgttat gtttttgcag
    86521 ccccagcgct accagcaggt tcatacaggc gattgctgcg gcacccgcgc cggaaaccac
    86581 catccgcacg tcggagatgt ttttctccac cacgcgcaag ccgttgagga tggcggcagt
    86641 gctgatgatt gccgtgccgt gctgatcgtc gtggaatacc ggaatattca tccgctcgcg
    86701 cagtttctgt tcaatataga aacattctgg cgctttaatg tcttcgaggt tgatgccgcc
    86761 gaaggtcggt tcgagcgcgg cgacgacttc aataaatttg tccgggtcga gttcatcaac
    86821 ttcaatgtca aatacatcaa tcccagcgaa tttcttaaat agaacgccct tgccttccat
    86881 caccggtttg cctgccagcg cgccaatgtt gcctaacccc agcaccgccg taccgttaga
    86941 gatcaccgca accaggttac cgcgcgcggt atatttgtag gcttttagcg ggtctttttc
    87001 gatttcaaga caaggtgcgg caacgcctgg tgagtaggcc agcgccagat cgcgctgcgt
    87061 tgccagaggc ttggttggag aaacctggat tttccctgga actggaaatt catggaaatc
    87121 aagtgcactt tgttttaact ggtcatccat ttggttgttc ctttcacgta acgttcacaa
    87181 ataaagtgtg tgggcaacag cccctgccca caacgtggcg cacattatta ccctgccgga
    87241 gtctacagac tttgagcaag tccaaactct caccattaat ataatgtttt ggtaataatc
    87301 ctataacact gatgttacct gcttaatcca gcaataccat gcctgtctgc tatgcttttt
    87361 tgatgcgttt agcgaaattt ctcagaagtg tgaattaacg cactcatcta acactttact
    87421 tttcaaggag tatttcctat gaacgagtta gacggcatca aacagttcac cactgtcgtg
    87481 gcagacagct gcgatattga gtccattcgc cattatcatc cccaggatgc caccactaat
    87541 ccttcgctgt tactcaaggc tgccggatta tcacaatatg agcatttaat agacgatgct
    87601 atcgcctggg gtaaaaaaaa tggcaagacc caggaacaac aggtggtcgc agcgtgtgac
    87661 aaactggcgg tcaatttcgg tgctgaaatt ctcaaaatcg tacccggttg cgtgtcaaca
    87721 gaagttgatg cacgcctctc ttttgataaa gaaaagagta ttgagaaggc gcgccatctg
    87781 gtggacttgt atcagcaaca aggcgttgag aaatcacgca ttttgatcaa actggcttcg
    87841 acctgggaag gaattcgcgc ggccgaagag ctggaaaaag aaggtattaa ctgcaacctg
    87901 acgctgctgt tttcttttac ccaggcgcgg gcctgtgcgg aagcaggcgt ttttctgatt
    87961 tcgccgtttg tagggcgtat ttatgactgg tatcaggcac gcaagccgat ggacccgtat
    88021 gtggtggaag aagatccggg cgttaaatcg gtgcgcaata tctacgacta ctataagcaa
    88081 caccactatg aaaccattgt gatgggcgcg agcttccgtc gcaccgaaca aatcctcgcc
    88141 ttaaccggct gcgatcgact gactatcgca ccgaatttgc tgaaggagct gcaggaaaaa
    88201 gtttctccag tggtacgtaa attaatcccg ccttctcaga cgttcccacg cccagcaccc
    88261 atgagcgaag cggagttccg ttgggagcac aatcaggatg cgatggcggt agaaaaactg
    88321 tctgaaggca ttcgtctgtt cgctgttgat caacgcaaac tggaagatct tcttgccgcc
    88381 aaactataaa ccagccacgg agtgttatat gtcccgaaaa gaccttgcca atgcgattcg
    88441 cgcactcagt atggatgctg tacaaaaagc caattctggt catcccggcg cgccgatggg
    88501 catggctgat attgccgaag tgctgtggaa cgattttctt aaacataatc ctaccgaccc
    88561 aacctggtat gatcgcgacc gctttattct ttccaacggt cacgcgtcga tgctgctcta
    88621 cagtttgcta catctgaccg gttacgacct gccgctggaa gaactgaaga acttccgtca
    88681 gttgcattcg aaaaccccag gccacccgga gattggctat acgccaggcg ttgaaaccac
    88741 caccggcccg cttggacaag gtttggcgaa cgccgtcggg ctggcgatag cagagcgtac
    88801 actggcggcg cagtttaacc agccagacca tgagatcgtc gatcacttca cctatgtgtt
    88861 tatgggcgac ggctgcctga tggaaggtat ttcccacgaa gtctgttcgc tggcaggcac
    88921 gctgggacta ggcaagctga ttggttttta cgatcacaac ggtatttcca tcgacggtga
    88981 aacagaaggc tggtttaccg acgatacggc aaaacgtttt gaagcctatc actggcatgt
    89041 gatccatgaa atcgacggtc acgatccgca ggcggtgaag gaagcgatcc ttgaagcgca
    89101 aagcgtgaaa gataagccgt cgctgattat ctgccgtacg gtgattggct ttggttcgcc
    89161 gaataaagca ggtaaggaag aggcgcacgg cgcaccactg ggggaagaag aagtggcgct
    89221 ggcacggcaa aaactgggct ggcaccatcc gccatttgag atccctaaag agatttatca
    89281 cgcctgggat gcccgtgaaa aaggcgaaaa agcgcagcag agctggaatg agaagtttgc
    89341 cgcctataaa aaggctcatc cgcaactggc agaagagttt ccccgacgga tgagcggtgg
    89401 tttaccgaag gactgggaga aaacgactca gaaatatatc aatgagttac aggcaaatcc
    89461 ggcgaaaatc gctacccgta aggcttcgca aaatacgctt aacgcttacg ggccgatgct
    89521 gcctgagttg ctcggcggtt cggcggatct ggctcccagc aacctgacca tctggaaagg
    89581 ttctgtttcg ctgaaggaag atccggcggg caactacatt cactacgggg tgcgtgaatt
    89641 tggcatgacc gctatcgcca acggcatcgc gcaccacggc ggctttgtgc cgtataccgc
    89701 gacgttcctg atgtttgttg aatacgcccg taacgccgcg cggatggcgg cactgatgaa
    89761 agcgcggcag attatggttt atacccacga ctcaattggc ctgggcgaag atggtccgac
    89821 gcaccaggct gttgagcaac tggccagcct gcgcttaacg ccaaatttca gcacctggcg
    89881 accgtgcgat cagatggaag cggcggtggg ctggaagctg gcggttgagc gccacaacgg
    89941 accgacggca ctgatcctct caaggcagaa tctggcccag gtggaacgta cgccggatca
    90001 ggttaaagag attgctcgtg gcggttatgt gctgaaagac agcggcggta agccagatat
    90061 tattctgatt gccaccggtt cagagatgga aattaccctg caagcggcag agaaattagc
    90121 aggagaaggt cgcaatgtac gcgtagtttc cctgccctcg accgatattt tcgacgccca
    90181 ggatgaggaa tatcgggagt cggtgttgcc ttctaacgtt gcggctcgcg tggcggtgga
    90241 agcaggtatt gccgattact ggtacaagta tgttggtctg aaaggggcaa ttgtcgggat
    90301 gacgggttac ggggaatctg ctccggcgga taagctgttc ccgttctttg gctttaccgc
    90361 cgagaatatt gtggcaaaag cgcataaggt gctgggagtg aaaggtgcct gatggtgatc
    90421 gccggatgct gtatgttccc ctcaccctaa ccctctcccc agaagggcga ggggacggtc
    90481 cgtgcgcttt ttagggccgc atctgcgatt ttgtttaacg ggtgatccac aacgtgggcc
    90541 tggcgtctgg cccatgccag ttatcgcagg taggttctgc ggcgtaacgc accaggcgaa
    90601 aacgctgacc gtcaaagcgc cagcgcgcct gaatgccaca atcgcttaat ccgcgccctt
    90661 tcgctaaggt caccagttca cgcgatttct catcaaatgt tgcgttcatc agttccagtt
    90721 cattcgtctc ctggccgttg ttgaacggca aacgcaaccg aaccgggcgc gaggctagcg
    90781 gctttttacg cgacacaatc catgccaaat caatggtgtt ataggcccct gcctcacagc
    90841 taatcatcat cagtgcttta tcatcggtca gcgcagtgac attcacctca cgacgtaacg
    90901 gatcaagcga gcagcgcaga ccattcatcc gccagtttcc ataatccagc aaatcgttac
    90961 gttcttcgag tgagagtggc gtcggcgttg ggttaaccac cgcgacctct ttcagcgcag
    91021 gcgcgggcgg tacgctgaga ggcggttcgt cccctttctt gatccacgcg gtttcactgc
    91081 caacgcgctt ttgctgagca tcaataaaca acaacgccgc ttttaagccg ctcagagaaa
    91141 tggtctgatt gccgtcgcgt aaggtgatcg ccttcccttc ctgaattatc tgcaaaaacg
    91201 cggtgatggt tgccgtatca tcggttacta ataaccatgg tgaaatccgc cacttgtcac
    91261 cacttaatgc caacggctca ccatctaata gcagccgtgg cgctatctcc ccttcagacg
    91321 catccggcga cttcaaccca ccgcgctcaa tacgtaaaac ggcatcggta tgcgccccag
    91381 cgctgcggct cagggtcatc accagtccat tatgatcgcc cgtattgcgc gccacacaaa
    91441 aattttgatt attacaggtg acctgccagt cggaaaacgc ccgttgcgct ggtgccgccc
    91501 acaccaaaga cgtcggcaac aaagcgaaaa aaaagagaag gaaaatgcga tagcgcatgg
    91561 acggtacgac cccagaagtt caaacaaaca gcacaagtcg tatcttcgtt gcgcaggtag
    91621 cgcggctcaa tcggatttgt cggattgagc caataataaa caacgaaata ttattgctat
    91681 ttttcagtcc attaaatgtg acatttgcaa atagttaagc aataacaccg tcttaccgtc
    91741 acgtatctcg ccggttttga tcatctccag cgcctggctg aacggcagct cgagcacttc
    91801 aatatcttca tcttcaacac cgccaccggc gttagcgcgc tgattgtcac tgtattcggc
    91861 gataaaaaag tggattagct cagtcacacc gccaggcgac atatacagtt caaataattt
    91921 gcgcacttcg cccacttcat agcccgtctc ttcaatcgct tctttacgaa tgcacacttc
    91981 cggttcgtcg ttatccagca gcccggcgca ggtttcaatc agctgcccgc tttcattgcc
    92041 attaacccag gtagcgacac ggaactgacg aatcagaacc acgctctttt tcttcgcgtt
    92101 gtacaggagg atcgtcgcac cattaccgcg atcgtaaact tcacgtttat ggcggataac
    92161 ttcgccatct ttgcgcgtga gatcgtaagt aatgttgtgc agggtgaaat agttatcgga
    92221 gagaattttg tctttaatga gggtgatttg ttgcgtcata ccgactccac agcgcgaaat
    92281 gaacaattat attacgctgt gaagtcgggt ttgtctgcgc aggctatcag tgagatttga
    92341 ctgattttac ccccagccag tcaataatcc cctgtgccgc atgacgtcct tctgccattg
    92401 cggtaaccac cagatccgca ccacgcacgg cgtcgccacc ggcgaagatt ttcggattgg
    92461 tggtctggta acggtactgg ctttccacat ccgcgatgat gcggccccat ttgtctaccg
    92521 ttacaccgtg cgactccagc cacggcatcc cgtgtggatt gaagccaaac gccataatca
    92581 ccgcatcggc tggcatgaca aattcactgc cctcaatcgg tacaggtcgc cgacgcccct
    92641 gggcatccgg ctcgccaaga cgcgtgcgca ggaaacgaat tccgcaaacg tgaccttgct
    92701 cattcagctc aagcgccacc ggctggacgt taaattcgaa gttggccccc tcttcgcggg
    92761 cgttcttcac ctctttcttc gagcctggca tgttagcttc atcacgacga taagcgcagg
    92821 tgacgttact cgcgccgtgg cgcagcgcag tacgcacaca gtccatcgcg gtgtcgccgc
    92881 cgcccagtac cacgacgtta agtccggcgg tattgataaa cggctcttcc ggtagctctt
    92941 cgagccccat cacctgttta gtgttggcaa tgaggaacgg cagcgcgtca taaacgcccg
    93001 gcgcatcttc attgggtaaa cccgctttca tggaacggta agtgcctacg ccaacgaaga
    93061 ccgcgtcgta ttgttccaaa agcaaatcca aagagacatc tttacccact tcacaattga
    93121 gttcgaagtg aatccccatc gcgctgaaga tttcccgacg gcgtgccagc agggatttat
    93181 ccagtttgaa agaaggaatg ccgaaagtga gcaagccacc gatttctgga tggcgatcgt
    93241 acaccgtcac cgccacgcca ttgcgggtca gaacatccgc acaggccagc cctgccggac
    93301 ctgccccgat aatcgccacc cgcttgtcca ctttggtgac atgacttaag tcaggacgcc
    93361 aacctttcgc caacgcctga tctgaaatat agcgttcaat gttgccgata gttaccgcgc
    93421 cgtgctcatc gcgaatagta caggcacctt cacacaaacg gtcttgcgga caaacgcgtc
    93481 cggtaatttc cggcagggtg ttggtctggt gagaaagctc gacggcggcg tcgatgtttc
    93541 cggctttcac cagttcaatc cactgcggaa tatggttatg cagcgggcag gtccattcac
    93601 aaacgctgtg ctcgccgaac ttaaggcagc gcgaggcttc ccgttgtgcc tggtcggcgc
    93661 gaaatggcag ataaatttca tcaaaaccgg ttttgcgcgc ttcaatcgcc agtttatccg
    93721 gctcgccacg cgcgggcgtt gcctgcattt gttcgacttt actcattacc ggcatttctt
    93781 gtgcagcggt actggcatgc cacggttgat gctcctgacg tgcggtgcgc aagcggcggg
    93841 atttcgccat gccggagagt gcgacgtcag tgaccagttg cagcgcgtcc gccgggcaat
    93901 tctcgacaca ggcaggaccg ttttcgcgcc ctgcacaaag gtcgcattta tgcgccgtgg
    93961 cttttacttt tcctgccgcg acgggtgtca ggacgatttg catcgtacca aacggacagg
    94021 ccaccacgca ggatttacag ccaatacact tttgctgatt gacctgaatg ctgtcatcaa
    94081 cgtggctgat tgcgccatta gggcagctac gggcgcaggg cgcatcttca caatggtgac
    94141 aggtcactgc actacgttgc tgctgatgtt tgataaccgt aattcgggga tgaaaatggt
    94201 gttggctcag gacatgttgc tcatcattgt gagccatgac acaggcgatt tcacaagcat
    94261 gacaacccag acattgctga ctgttggcca taataaaacg attcataacg accttctttt
    94321 ttggttgtaa aaaccttatt ctttatatga gtgttgttat tacccgactt acaggggaac
    94381 cggcaatgtt catgtgccca gaataagtaa ctatttcgct ataaactgtg gcagatcaaa
    94441 taatcccatc actgactaaa ttgcgtttca gcgaactgga acattaatga ttttttgtgg
    94501 agaagacgcg tgtgattgtt aaacgacccg tctcggccag tctggcccgg gccttttttt
    94561 acattgtgct gctgtcgatt ctttccacgg gtatcgctct gctaactctg gcgagcagtt
    94621 tgcgcgacgc tgaggctatc aatattgccg gatcgctgcg tatgcagagt taccgcctgg
    94681 gctacgactt gcaaagtggc agtccacaac tcaatgcaca tcgccagttg tttcagcagg
    94741 cactgcattc accggtatta accaacctca acgtctggta tgtgccagaa gcagtaaaaa
    94801 ctcgctatgc gcatctgaat gccaactggc tggagatgaa taatcggctc agcaagggcg
    94861 atttgccgtg gtatcaggcc aatattaata attatgttaa tcagatagac ctgttcgtgc
    94921 tggctttaca gcactacgct gaacgcaaaa tgctgctggt ggtggcgatt tccctggctg
    94981 gcggtatcgg tattttcacg ctggtctttt ttactttgcg ccgcatacgc catcaggtgg
    95041 ttgccccgct gaatcagctg gttaccgcca gtcagcgtat tgaacacggg cagttcgact
    95101 cgccgccgct ggataccagt ctgccgaatg agcttggtct gcttgcaaaa acctttaacc
    95161 aaatgtcgag cgagctgcat aaattgtacc tttcgctgga ggcgtcagta gaagaaaaga
    95221 cccgcgatct ccacgagacc aagcgtcgtc tggaggtgtt gtatcagtgc agtgttccca
    95281 ggcgctgaac accagccaga ttgatgtgca ttgtttccgc catattttgc agattgttcg
    95341 cgacaatgaa gcagctgaat atctggagct gaacgttggg gataactggc ggattagcga
    95401 agggaagcca aaccctgagc tgccaatgca gattttaccg gtgacaatgc aagagacggt
    95461 ttacggcgaa ctgcactggc aaaatagtca cgtttcatca tcagaaccgc tgcttaacag
    95521 tgtttcatcg atgctgggac gcggtttgta ctttaatcag gcgcagaagc attttcagca
    95581 gttattgttg atggaagaac gtgcgaccat cgcccgcgaa ttgcacgact cgctggctca
    95641 ggtgctttct tacttacgta tccagttgac gttactgaag cgttcgatac cggaagataa
    95701 cgccaccgca caaagtatca tggccgattt ttcccaggcg ttgaatgatg cttatcggca
    95761 gttacgcgag ctgttgacta ctttccgcct gacgctgcag caggcggatc tcccctccgc
    95821 gttgagggaa atgctggata cgttacaaaa tcaaaccagc gctaaactga ccctcgactg
    95881 ccgtctgcca accctggcac tggatgcgca aatgcaggtg catttgttgc aaattattcg
    95941 cgaagcggtg ctgaatgcga tgaagcacgc caacgccagc gaaatcgccg tcagttgcgt
    96001 caccgcgccg gacggcaatc acacggttta tatccgtgat aacgggattg gtatcggtga
    96061 accgaaagaa cccgaaggtc attatggact gaatatcatg cgcgaacgcg cggaacggct
    96121 gggcgggacg ctgacttttt cacaaccttc cggcggcggc acgttagtga gtattagctt
    96181 tcgctccgca gagggtgagg ggaaggggaa cacgccggct gaggtgtaaa taattaccct
    96241 cacaccggcc ctctcccttt aaaggcgaga gggaaaaacg tgcccatatt gacctgtaag
    96301 cgagtgtgtc agcaatctca tgataattta cattaactcc tttttttctc cacgattggc
    96361 tcgtaccttg ccgctacagt gaagcaagtc aagcctacaa caatacgcag aaacacgagg
    96421 tcctctttta atggcgaatt tctttattga tcgccccatt tttgcctggg tgctgacaat
    96481 cctgttgtgt ctgacaggta ccctggcgat tttttcattg cccgttgaac aataccccga
    96541 tctcgcgcca ccgaatgtgc gagtgaccgc taactatccc ggcgcatcgg cccagacgct
    96601 ggaaaacacc gtgacccagg ttatcgagca aaatatgacc ggcctcgata atctgatgta
    96661 tatgtcatct cagagcagtg gcaccggtca ggcatctgtc actttaagtt ttaaagcagg
    96721 caccgatccg gacgaagccg tgcagcaagt acaaaaccag ctgcaatcag ccatgcgaaa
    96781 gttaccgcag gcggtgcaaa atcagggcgt gacggtgcgt aaaaccggcg ataccaacat
    96841 tctgaccatt gccttcgtct ctaccgatgg ctcgatggat aaacaggata ttgctgatta
    96901 tgttgccagt aatattcagg acccgttaag ccgcgtgaat ggcgtcgggg atatcgatgc
    96961 ctatggttcg caatattcca tgcgtatctg gctggacccg gcgaaactca acagtttcca
    97021 gatgacggct aaagatgtca ctgatgccat tgagtcacag aacgcgcaga ttgcggttgg
    97081 gcaacttggt ggtacacctt ccgtcgataa gcaggcgctc aacgccacca ttaacgccca
    97141 gtcactgctg caaacaccag aacagttccg cgatatcacc ttgcgggtga atcaggacgg
    97201 ctcagaggta aggctgggcg atgtcgccac cgtcgaaatg ggggcggaga aatacgatta
    97261 tcttagccgc ttcaatggta agccagcctc cgggttgggg gtaaaactgg cctccagcgc
    97321 aaacgaaatg gcgacagcgg agctggtgct caatcgtctc gacgagctgg cgcagtattt
    97381 cccgcacgga ctggaataca aggtggcgta tgaaaccacc tcgtttgtta aagcctccat
    97441 tgaagacgtg gtgaaaacgc tgctggaagc tatcgctctg gttttcctcg ttatgtatct
    97501 gttcctgcaa aacttccgcg ccacgctgat acccactatc gccgtgccgg tggtgttgat
    97561 gggaaccttc tccgtacttt acgccttcgg ttacagcgtc aacaccttaa ccatgttcgc
    97621 gatggtgctg gcgatcggtc tgctggtgga tgacgccatc gtggtggtgg aaaacgtcga
    97681 acgtattatg agtgaggaag gactcactcc tcgcgaagcc acacgtaaat cgatggggca
    97741 gatccagggg gcactggtcg ggattgcgat ggtgctttcg gcggtatttg taccaatggc
    97801 cttcttcggc ggcaccaccg gtgccatcta tcgccagttc tctattacca ttgttgcggc
    97861 gatggtgctg tcagtactgg tagcgatgct cctcactccg gctctgtgtg ccacgctact
    97921 taagccactg aaaaaaggtg agcatcacgg gcaaaaaggt ttttttgcct ggtttaacca
    97981 gatgtttaac cgcaacgccg aacgctacga aaaaggggtg gcgaaaattc tccaccgtag
    98041 cctgcgctgg attgtgattt atgtcctgct gcttggcggc atggtgttcc tgttcctgcg
    98101 tttgccgacg tcgttcttac cgctggaaga ccggggcatg tttactacct cggtacaatt
    98161 gcccagcggt tcaacccaac aacagaccct gaaagtcgtt gagcaaatcg agaaatatta
    98221 cttcacccat gaaaaagaca acatcatgtc ggtgtttgcc accgttggtt ctggccctgg
    98281 gggtaacggg caaaacgtgg cgagaatgtt tatccgcctg aaagactgga gcgaacgcga
    98341 cagtaagacc ggcacctcgt ttgccattat cgagcgtgca acgaaggcgt ttaaccaaat
    98401 taaagaagct cgcgttatcg ccagcagccc accagcaatt agcggtcttg gtagttctgc
    98461 aggttttgat atggagttgc aggaccacgc tggagcgggt cacgatgcgc tgatggcagc
    98521 acgtaatcag ttgctggcgc tggcggcgga aaacccggag ctaacccgtg tgcgccataa
    98581 cggcctcgat gacagtccgc agttgcagat tgatatcgac cagcgtaaag ctcaggcgct
    98641 gggcgttgat atcgacgata ttaacgacac actgcaaacc gcctggggtt cgagctatgt
    98701 gaatgacttt atggatcgcg gtcgcgtgaa gaaagtctat gtgcaggcag ctgcgccgta
    98761 tcgcatgctg ccagatgaca tcaatctctg gtatgtccga aataaagatg gcggcatggt
    98821 gcccttctct gctttcgcga cctcacgctg ggaaacaggc tcgccgcgtc tggaacgcta
    98881 taacggttat tctgcggttg agattgttgg ggaagccgca ccgggggtca gtaccggtac
    98941 ggcgatggat attatggaat cgttagtgaa gcagctgcca aacggctttg gtctggagtg
    99001 gacggcgatg tcgtatcagg agcggctttc cggcgcgcag gctccggcgc tgtacgccat
    99061 ttccttgctg gtggtattcc tgtgtctggc ggcattgtat gaaagctggt cggtaccgtt
    99121 ctcggtaatg ctggtcgtgc cgctgggggt aatcggcgcg ctgctggcaa cctggatgcg
    99181 cgggctggaa aacgacgttt acttccaggt gggcctgtta acggtcattg gtttatcggc
    99241 gaaaaacgcc atcctgatcg tcgagtttgc taacgagatg aaccaaaaag gccatgacct
    99301 gtttgaagcg acgctccacg cctgccgtca gcgtttacgc ccgattctga tgacctcgct
    99361 ggcatttatc ttcggcgtat tgccaatggc aaccagcacg ggtgccggtt ccggtggtca
    99421 gcatgcggtg ggtactggcg taatgggcgg gatgatttca gctactattc tggctattta
    99481 cttcgtgccg ctgttctttg tgctggtgcg ccgccgcttc ccgctgaagc cgcgcccgga
    99541 ataagcaata aaaaaggcga catgccaatg tgtcgccttt ttcaactttc cgattaagaa
    99601 cctgctcagc gggttcttgc tgtttatact ttgtctcagg aattacttac gaagcataac
    99661 ttcgataaag tctttccagt tccccagttc acgttcaatc ataacaacct ctcttataat
    99721 tatgggtatt ctacggaaac aatataccgt ggtgaagcta atttactcga ttgctgcgat
    99781 gactacctcc gggggacaaa ccttatgtaa atactatggt cctgcggtga taatttgtat
    99841 gtgatacaca gcaacatttc gagatattca tacggcatct aatactgatt taattctggt
    99901 taaaatacag acagataaca agatgaatat tcttaatgtt tacgttaaaa atgtttaata
    99961 ttatttaata gttgttaatt tgaatacttc gataatgtta tattttctga taatcatttg
   100021 caggcaaaat gttttcaccc ttaaatgagt atttattctc ataaatcgaa aaaggattca
   100081 ttatggttac actttacggc atcaaaaatt gtgacaccat taaaaaggct cgccgttggc
   100141 tggaagccaa taacatcgac tatcgttttc atgattaccg cgtcgatggg ctggacagcg
   100201 aattattgaa cggttttatc aacgaattag gctgggaagc gttactcaac acccgtggta
   100261 caacctggcg taaactggac gaaactaccc gcaataaaat caccgatgcg gcctctgcgg
   100321 cggcattaat gactgaaatg cctgcaatta tcaaacgtcc attgctctgc gcgcctggta
   100381 agcctatgct gctgggtttc agtgattcca gttatcagca atttttccat gaggtgtagt
   100441 ctatgtcgtg cccggttatt gagctgacac aacagcttat tcgccgccct tccctgagtc
   100501 ctgatgacgc aggatgtcag gctttgttga ttgaacgttt gcaggcgatc ggttttaccg
   100561 ttgaacgcat ggattttgcc gatacgcaga atttttgggc atggcgtggg caaggtgaaa
   100621 cgttagcctt tgccgggcat accgacgtgg tgccgcctgg cgacgccgat cgttggatca
   100681 atccgccgtt tgaacccacc attcgtgacg gcatgttatt cgggcgcggt gcggcagata
   100741 tgaaaggctc gctggcggcg atggtggtag cagcagaacg ttttgtcgca caacatccca
   100801 accatacggg gcgactggca tttctgatca cttctgatga agaagccagt gcccacaacg
   100861 gtacggtaaa agtcgtcgag gcgttaatgg cacgtaatga gcgtctcgat tactgcctgg
   100921 tcggcgaacc gtcgagtatc gaagtggtag gtgatgtggt gaaaaatggt cgtcgcggat
   100981 cgttaacctg caacctcacc attcatggcg ttcaggggca tgttgcctac ccacatctgg
   101041 ctgacaatcc ggtacatcgc gcagcacctt tccttaatga attagtggct attgagtggg
   101101 atcagggcaa tgaattcttc ccggcgacca gtatgcagat tgccaatatt caggcgggaa
   101161 cgggcagtaa caatgttatt ccgggtgaac tgtttgtgca gtttaacttc cgcttcagca
   101221 ccgaactgac tgatgagatg atcaaagcgc aggtacttgc cctgcttgaa aaacatcaac
   101281 tgcgctatac ggtggattgg tggctttccg ggcagccatt tttgaccgcg cgcggtaaac
   101341 tggtggatgc ggtcgttaac gcggttgagc actataatga aattaaaccg cagctactga
   101401 ccacaggcgg aacgtccgac gggcgcttta ttgctcgcat gggggcgcag gtggtggaac
   101461 tcgggccggt caatgccact attcataaaa ttaatgaatg tgtgaacgct gccgacctgc
   101521 agctacttgc ccgtatgtat caacgtatca tggaacagct cgtcgcgtga tgagtggttc
   101581 tgcaagagga aataagcatg gactggctgg ctaaatattg gtggattctg gtgattgtct
   101641 ttttggtagg cgtcctgctg aacgtgatta aagatctcaa gcgcgtcgac cataagaaat
   101701 ttctcgccaa caagccggag cttcccccgc atcgtgattt caacgataag tgggacgatg
   101761 acgacgactg gccgaaaaag gatcaaccga agaagtaagc tgagtgctca accgtaggcc
   101821 ggataaggcg ttaacgccgc atccggcaat ggtgaacgat ccctgatgcg acgctcacgc
   101881 gtcttatcag gccaggtaat ctgtctttta catcatttca atcacatcat catcgccagg
   101941 cttaccgcca cttagcgctt ggtcgaaata atgtttcgga atggtataac gcaagtgatc
   102001 cagggcaaat tgcatactgc gattgtcgat cgcatggcct aaatcctcca cgatatccag
   102061 cgtcacgtca ccaccggcac tgattaacgc ttcctgcgcc gcaactgcat gcgccagatc
   102121 gataaccgga tcttcaccac catgaatcaa gtgaattgtc gtggctgtag acgcggtctc
   102181 cggcaggctc gcatagcgtc cgttaaaggc aatgacgcgc gaagcaaggc ccggttcggc
   102241 tttaatgctc tccagcgcca taatcgcgcc ttgtgagaaa ccaattagcg ccgtggcgtt
   102301 agcccctact ccgctctgtt tctgccagta gcgtaccgtt tcaataaacg tcggcatgat
   102361 ggcatccaca cgcgcctggc gattatcttc cgtaataccc tgcactgaaa accactgacg
   102421 ccccgccgga ttaccgctcg gctccgcgcc accgacactc accaccagcg catcaggaaa
   102481 cagcggtgca aaccagctgc ctatttcccc cattgccacc gggttatccc cgacaccgtg
   102541 gaaaagcagt aacaactgtt gtgcaggttt atccgggctt tgaacaacaa aatggtcatg
   102601 tttcatggcg aactccttaa ctgatgtcat taattttacg ccgctgactc acaatgacca
   102661 tgggagaaaa ttgaaggagt cagtgaaaaa attgccattg cgttatgcga tcgcgcagac
   102721 gctcagtgcg aacctcattt agtgcgaaca gcgcctgcgc ggcctcttcc cgctggcgaa
   102781 ccagtaacat cttgcggcct gaaagtttaa gtgtggtaca taactgcgcg tcactggcgt
   102841 ttttctgtaa acgcccacgc agcgccggta atgccagttc actggtttgt agcagacgca
   102901 ataagcaacc taacgatgtt aatagcggac gatgagcgaa agcaaaaccg gccagttcaa
   102961 gccagtcgtc gtcagaaagg atggcatcgt ttagtggatc aacaggaagc atttcgccat
   103021 tccactgcgc gagagcttgc gcatcgcgac gtaaacggta atgctcacgt tcagccagct
   103081 gtttacccgc atcactcatc ggtaacagcg ccatcgccgt atagcaaccg ctgctggctt
   103141 cccgatgatt acccatccgc accagcacaa aaccgcagcg ttgccagaaa cgccataact
   103201 ccccagtgta accaaaactc accgaaagat agtcgaggtc atgcgtatat tgcaaagctc
   103261 cagcaataag ttgtcgccct gtgccttccc gctgacgagc cggatgaact gctatccggc
   103321 taacccgcct tccgcgcaat gtcgccgcca gtggattgct gccgtgcgcc gccagcgact
   103381 gggccaccag attaccccgc gggcgacgaa aacctgccca taccgcctga ctgagttctt
   103441 gagataatcc gccctcatcc accagccaca acgccccggc aatctcgttt tcgccagccg
   103501 cctgtaaaaa atgttgccct ggtgcatcca tcatccggcg taaatccagc ggcgaggtcc
   103561 ggtagtgcgc accagacaat aactgataaa cctttaacgg cgtttctggc tcgcttcgcc
   103621 ataacgtttg ttcaaatgcg gaaatgacga tattgccttg tggtgtatgg gtgaagtttt
   103681 catcgttaaa aaccagtgcc tcactaacca ttttttccag cgggcatccc tgtgcccagc
   103741 ggatcggctg ttgcagttca aaacggtgta aatgcggaaa gcgagcgcaa aatttcagca
   103801 aaaaaccacg tccggtacct tcgtagccct gcaccgtagt ggttaacaac gttcgaggaa
   103861 aacgcgatac cagttgatgc aacaacggcg caggtatggc tgcggcttcg tcgaccacca
   103921 gccagtcggc ttgctcatcg ctggctaaca aggcatccgg tgcaataaag cgaaacttct
   103981 cgcccgcaaa ttgtgccagt acatccgttg ccgcttttgc gggcgcggtg acaatcgcac
   104041 tgcccgcaat acgagaaatg agttgccctg ccaacgccga cttaccgcgc ccacgcgcag
   104101 ccgttaccgc tgccacgccc ggcggcatgg tcagtagctg ctgtaagagt tgctgttgtt
   104161 ctggttgtgg tgcgccggtc gcggggtgcc agtcagtacg gggagtaaaa tgcgccaacg
   104221 agaacggctg gttttgccgc cagaggatag cgtcgttatc cgccgtaagt acgcgtttga
   104281 gatgctggac aaaatgcggc gtcgcaatag ggtcagggca atcactccag cgcagcgagt
   104341 cggcatcagg ttggttttcc cactcttccc atacagggag taacaaaacc agccagcttc
   104401 ccgctttcaa cgttccgcta agtgctgcaa aggcagcggc atcaaagccg tggcgggcgt
   104461 cgaataccgc atgccggaac tcgcgcccaa gtaaagtttg tagcgccgag ggtgaacagt
   104521 ggttttcagc atctagctgc ggcgaaatcc acagccagtc gccaggtaag gcatcacgca
   104581 acttaagagc atgatcaaaa caccaaccct cttccccgct caacaccagc aagcggcaga
   104641 tcccttcacg tttcatttgc gctgttaatg tgtaaagcgc agtcagttca gccatccctg
   104701 ccccggaaat taaatgcttt taccaaaagt attgcattgt gccggatcgc cgctgtcgaa
   104761 accacgttta aaccagctgt agcgttgctg agaagtgcca tgtgtgaaac tgtctggtac
   104821 tactcgcccc tgactttgct gttgtaaacg gtcatcgccg atggcctgcg ccgcattcag
   104881 cgcctcttcc agatcgccag tttccagaac gccttgctgc tgcatactat gcccccagac
   104941 accggcaaaa cagtcggcct ggagttccat acgcacagat aagcggttta cttccgcctg
   105001 cgtcgcgttt tgttgcagtt gacgaacttt cggctcgatg cctaacagtt tctgcacatg
   105061 atgaccgact tcatgggcga taacgtaccc ctgggcaaaa tcgccatccg cgccaagttt
   105121 gtctttcatg tcatcataga aggagagatc gatataaact gtgccatccg ccgggcaata
   105181 gaacggcccc ataatggact ggcccgcccc gcagccggtg cgcgtcattc cacggtacat
   105241 gaccagcttc ggttgctgat aggtcttacc catcttttcg aactgttgtc cccaggtgtc
   105301 ttccgtggtt gcctgaatca ccgaggtgaa ttttgcggct tcgtcttcat tagggctaat
   105361 tgaccgcgtt gattgttgtt gagaaaccgg ctgcccggtc atcaacccgg ttaaatcaac
   105421 gccatagtag cctgcaacca gcacgactat cagtaaaatc agcccgcctt taccgctcgg
   105481 cagacggaaa ccgggaccgc ccattgacgg accgccagag ctgttgcgcc tgtcttcaac
   105541 attgtcactt tcacgtcgcc cttgccaacg catagatacc tcaacaatat attcattata
   105601 gaaatgatcg taggtggttc agaggaagat taccacagga aagcgggatg aaatggcgga
   105661 ggaatgagcg ttcaatgcat atattccaag agcggttgtc ttgccgtgcc agctgcacgg
   105721 caagatgatg aacagaaaaa tcagtccagc tgtacaccca ggcggcgggc gacggcttca
   105781 taggcttcga tcaggccacc gaggctctgg cggaaacggt ctttgtccat tttctccagt
   105841 gtttctttgt cccacaggcg gctgccgtcc ggggagaact catcgcccag taccacttca
   105901 cctttgtaca gaccaaactc cagcttgaag tcgaccagaa tcagaccagc atcatcgaac
   105961 agttttttca gcacgtcgtt cgctttgtag gtcagctctt tcatacgcgc caggttctct
   106021 ttgctcaccc agccaaaggt ttcgcagtaa gattcgttga ccatcggatc gtgcatggca
   106081 tcgtttttca ggaacagatc gaacagcggc gggttcagct caataccttc ttcgattcca
   106141 agacgtttca ctagagagcc agcagcacgg ttacgcacga cacactcaac cggcaccata
   106201 tccagctttt tcaccagaca ttcggtatcg gagagcagac gctccatttg agtcgggata
   106261 cccgcttcag ccagtttgct cataatgaag tagttgaact tgttgttcac catacctttg
   106321 cgatcaaact gctcaatgcg cgcgccatcc cctgctgacg tatcattgcg gaattcgagc
   106381 accaacaggt ccgggttttc cgtgctgtat acggttttcg ctttaccacg atacaactca
   106441 gcttgctttt gcatctttat cactcctggg tgtgaattaa cgttttaaaa tcttttgctg
   106501 tctggtgtgc cggatgtttt gtcggatgcg gcgtgaacgc cttatccgaa ctacgtccga
   106561 caaacaaatt tcgtgcgaat taccgctaaa atcgcgtttt cctgccgacg cacacgtttg
   106621 cgtatcatat cagaaaaaag ggccggatga ttccagccct gtatttttac ttgctaaacg
   106681 cagcctggaa gacagctacc agcgcgtcgt tctgactctg agtcagagta tgacctttcg
   106741 gatcgatgaa ctgtaggctg ctgcggttat ctaaatcgcc aacctgcagt ttatagtcac
   106801 cggatgccag gcctggatcg ctcgcgccca gttcctgcca gtcgctgtca gacagcggct
   106861 tataagttac ggccatgttg ccctgcgaac gggtgctgtc ggtcactttc atgcccactt
   106921 tttccagcgc cgctggcaga cgttgccaaa ccacattgaa cggcccgcgt acgaccagca
   106981 ttggtaaacc ggtgtcatca gctgcacttt gtacgtccat agtggtggag gcacgatttt
   107041 gcgcagcgtt cgcggcgtca gtggcagatt tatccagacc ggcggaaata acgttcatca
   107101 tctccgtgct gtaacgctgc atggaagccg cgtctgcaac cggtttgccc gcctgttcca
   107161 ggttcagcag tttaaccgta accgcctgct gataaccctg cggcttaaca gagatttgat
   107221 aacgaccacg atactgctcg tcttcgtcca gacggttcca ttgtacccaa tcggtggtca
   107281 gtgtctgacc agcatcatca cgttgggtga tggtgtagtt tttcgcctgc agcacgctaa
   107341 ccacctgcgg ccacagagta ttgccacgac cattttccac cagcaatgaa gcggtatcgc
   107401 ccgtgaactg ggtacgcgcg ccagaaacca gtgccaacgg ctgggctggt ggacgaatgt
   107461 ccagcgcctt accgacagca ccactaccgt tggtcaccgg gattgcataa tcaccggagg
   107521 tcaccggcaa aatcattcca gccggggcat gaagctccgc aagcggtgcc gcttccaggt
   107581 aggcttcatc accactgacc tgacgcttat agcgtgagtc agaactacag gcagcgagta
   107641 ataaaacaag cgaaacaccc gcaacctttg ccaggcgcga cttttgaaca gagtaagcca
   107701 tcaaatctcc ctaaacttta cagcaaaccg gcatgcttaa gcgccgctct gaccgtctca
   107761 cgaccactgt cggtgattgg tgtcattggc aggcgcagcg tatcggtcgc cacaagaccc
   107821 agttccttac atgcccattt caccgggatt ggattgggtt cgacaaatag tttgttgtgt
   107881 aatggcatca gacgctgatt aataacgcgt gcctcggcaa aatgcccttc tgctgccagt
   107941 ttgcacatct gggccatatc acgcgctgcg acgttagccg taacggaaat aactccatga
   108001 ccgcccaatt gcatgaagtc cagcgcgctc gcatcatcgc cgctcagcag aacaaaatca
   108061 tctgaaacca gctctttgat ctggtttaca cgcgttaagt tccctgttgc ctctttgatt
   108121 ccgataatat tttttacttt cgccagacgg cccaccgttt ccgggagcag atcgcagcca
   108181 gtacgggacg gcacattata cagaatttgc ggcaggtcag tatgctcagc gatggctttg
   108241 aaatgctgat acaaaccttc ttgcgacgga cgattgtagt aaggggttac cgtcaggcag
   108301 ccgacgatac cactgtcatt gaagcgctgc gtcaggctaa tggcttccgc agtagcgtta
   108361 gcgccggtcc cggcaattac cggaatgcgc ccatcagcca gatccagcgt catcatcacc
   108421 acatcagcat gttcgtcatg atttaaggta gcggactcgc cagtggtgcc aacagaaacg
   108481 atcgccgaag taccgctggc gacatgataa tcaatcagtt ttttcaagct agcccgacag
   108541 acattacctt tttcatccat cggagtaaca atcgcgacaa tacttcccgt gaacatgggc
   108601 catcctctgt gcaaacaagt gtctcaatgg tacgtttggt atggcattaa aagcaagcag
   108661 acagaaccgt tctgattgtt gtatgcatga tttttttatg ctttccttaa gaacaactca
   108721 ccccttaaag gaataaccag tttgacactg tcatcgcaac attatctggt gatcactgcg
   108781 ttgggtgccg atcgccctgg aattgtgaac accatcaccc gtcatgtcag tagttgcggc
   108841 tgtaatattg aagacagtcg cctggcgatg ctgggagaag agttcacgtt tatcatgctg
   108901 ctttccggtt catggaatgc catcactctg attgaatcaa cgttaccgtt gaaaggtgcc
   108961 gaactggatc ttttaatcgt gatgaagcgc acgacggcgc gtccgcgtcc gccaatgcca
   109021 gcatctgtct gggttcaggt cgatgttgca gactccccgc atttaattga acgcttcaca
   109081 gcacttttcg acgcgcatca tatgaacatt gcggagctgg tgtcgcgcac acaacctgct
   109141 gaaaatgaac gggctgcgca gttgcatatt cagataaccg cccacagccc cgcatctgcg
   109201 gacgcagcaa atattgagca agcgttcaaa gccctatgta cagaactcaa tgcacaaggc
   109261 agtattaacg tcgtcaatta ttcccaacat gatgaacagg atggagttaa gtaatgaatc
   109321 cactgaaagc cggtgatatc gcaccgaaat ttagcttgcc ggatcaagac ggagaacaag
   109381 ttaatttgac cgacttccag ggacagcgtg ttctggttta tttctacccg aaagccatga
   109441 cccccggctg taccgtacag gcctgcggct tacgcgataa catggatgag ttgaaaaaag
   109501 cgggcgttga tgtgctgggt atcagcaccg ataaacccga aaaactctcc cgttttgcgg
   109561 aaaaagagct gcttaacttt acgctcctgt ctgatgagga ccaccaggtg tgcgaacaat
   109621 tcggcgtctg gggtgaaaag tccttcatgg gcaaaaccta cgatggtatt catcgcatca
   109681 gcttcctgat tgacgctgat ggcaaaatcg aacatgtctt tgacgatttc aaaaccagca
   109741 atcaccacga cgttgtgctg aactggctga aagaacacgc ctgattactt tgctccattc
   109801 cgtgctggct gcgcttgcgg ccagcatacc tcacttctcg tgatcaagat cacattctcg
   109861 ctttcccctg cgacacgggt gtcgaatcca ttttttgctg aacgttaatg accatcattt
   109921 ttgtaccgtt cagaatccag ttaatacata acttattgaa tagattgagt taatcagaat
   109981 ggcatccttt atacaatatg aaatgcaatg tttcatatca ttttcaagga gccgacatga
   110041 accgctttgt ggtggccgaa ccactgtggt gtacaggatg taatacctgt ctcgctgcct
   110101 gttcggacgt gcataaaacg caaggtttac agcaacaccc gcgcctggcc ctggcgaaga
   110161 cgtcaacaat cactgcccct gtcgtgtgtc atcactgtga ggaagcccct tgcctgcagg
   110221 tctgcccggt caatgccatc tctcagaggg atgatgcgat ccaactcaac gaaagcctct
   110281 gtattggctg caagctttgc gccgtggtct gcccatttgg cgcaatcagc gcttcaggaa
   110341 gccgtccggt gaatgcccat gcgcaatatg tttttcaggc tgaaggctca ctcaaagacg
   110401 gcgaagaaaa cgtgccgccc caacatgcgt tgctgcgctg ggaaccgggt gtccagaccg
   110461 tcgcggtgaa atgcgacctg tgtgatttct tgccagaagg tccggcctgc gttcgcgctt
   110521 gcccgaatca ggcgttacgg ctgatcaccg acgatagcct gcaacgtcag atgaaagaaa
   110581 aacagcgcct tgccgcaagc tggtttgcca atggcgggga gtctcccctt tccctcactc
   110641 aggagcaaca ctaatggatg ccctgcaatt attaacctgg tcgctgatcc tctatctgtt
   110701 tgccagtctg gcttcgctgt ttttactcgg tctggacaga ctggctatta agctttccgg
   110761 catcacatcg ctggtgggcg gcgtgattgg catcatcagc ggaattacgc aattacatgc
   110821 tggtgtaact ttagtcgccc attttgccac cccttttgac tttgccgatt taaccctgcg
   110881 aatggatagc ctctcggcat ttatggtgct agttatctcc ttgctggtgg tggtttgttc
   110941 gctctattca ttgacttata tgcgcgaata cgagggcaaa ggcgcggcgg cgatgggctt
   111001 ctttatgaat cttttcatcg catcgatggt tgccctgctg gtgatggaca acgctttttg
   111061 gttcatcgtg ctgtttgaaa tgatgtcgct gtcttcctgg tttctggtca ttgccaggca
   111121 ggataaaacg tcgatcaacg ctggcatgct ctactttttt atcgcccacg ccggatcggt
   111181 gctgattatg atcgccttct tgctgatggg gcgcgaaagc ggcagcctcg attttgccag
   111241 tttcgcacgc tttcactttc tccggggctg gcgtcggcgg tgtttctgct ggcctttttc
   111301 ggttttggcg cgaaagccgg gatgatgccg ttgcacagct ggttgccgcg cgctcaccct
   111361 gccgcaccat cgcacgcttc ggcgttgatg tctggcgtaa tggtcaaaat cggtattttc
   111421 ggcatcctga aagtagcgat ggatctgctg gcgcaaacgg gtttgcctct gtggtggggc
   111481 attctggtga tggcgatcgg cgcaatctcc gcgctcctgg gcgtgctata tgcgctggcg
   111541 gaacaggata tcaaacggct gctggcctgg agtaccgtcg aaaacgtcgg cattattttg
   111601 ctggcagtcg gtgtggcgat ggacggtctg tcactgcacg acccgctgct caccgtggtt
   111661 ggactgctcg gcgcactgtt tcatctgctc aaccatgcgc tgttcaaagg gctgctattt
   111721 ctcggcgcgg acgcgattat ttcgcgtttg catacccacg acatggaaaa aatgggggca
   111781 ctggcgaaac ggatgccgtg gacagccgca gcatgcctga ttggttgcct cgcgatatca
   111841 gccattcctc cgctgaatgg ttttatcagc gaatggtaca cctggcagtc gctgttctca
   111901 ctaagtcgtg tggaagccgt agcgctacaa cttgcgggtc ctattgctat ggtaatgctg
   111961 gcagtcactg gtgggctggc agtaatgtgc ttcgtcaaaa tgtacggtat tactttctgt
   112021 ggtgcgccgc gcagtacaca cgctgaagag gcacaggaag tgccaaatac gatgatcgtc
   112081 gccatgctac tgctcgcggc actctgcgta ttaattgcgc ttagtgccag ttggctggca
   112141 ccgaagataa tgcatattgc ccatgcgttt accaataccc ctcccgtcac tgtcgccagc
   112201 ggaatagcac ttgtacccgg cacgtttcat acacgggtca ctccttcatt actgttgctg
   112261 ttactactgg cgatgccttt gctgccaggc ctttactggc tgtggtgtcg ttcgcgccgc
   112321 gcagcgtttc gtcgcacagg agatgcctgg gcatgcggct acggctggga aaacgcgatg
   112381 gctccgtcag gcaatggcgt gatgcagccg ctgcgtgtgg tcttctctgc gttatttcgt
   112441 ctacgacaac agctcgaccc tacgctgagg ctaaataaag gtcttgcgca cgtcaccgcc
   112501 agggctcaga gcacagaacc cttctgggat gagcgggtga tccgccccat cgtgagcgcc
   112561 acccaacggc tggtcaaaga aatacagcat ctgcaaagcg gcgactttcg tctctattgc
   112621 ctgtatgtgg tcgccgcact ggttgtgctg ctaatcgcta ttgccgtcta aggaaatcac
   112681 catgagacaa actctttgcg acggatatct ggtcattttt gcgttagcac aggccgtgat
   112741 tctgctgatg ctaaccccac tttttacggg tatttcccgg cagatacgcg cgcgtatgca
   112801 ctcccgccgc gggccgggga tctggcagga ttatcgcgat atccacaaac tgtttaaacg
   112861 ccaggaagtt gcgccgacat cttcaggtct gatgttccgc ctgatgccgt gggtattaat
   112921 cagcagcatg ctggtgctgg cgatggcctt accactattt attaccgttt ccccttttgc
   112981 gggcggcggc gatctgatca cccttatcta tcttcttgcc ctgtttcgtt ttttctttgc
   113041 tctttccggg ctggataccg gaagtccgtt tgcgggagtc ggtgccagtc gcgagttgac
   113101 gctcggcatt ctggtcgaac caatgcttat tctctcactg ctggtattgg cgctgatagc
   113161 aggttccacg catatcgaga tgatcagcaa tacgctggct atgggctgga actcgccgct
   113221 aaccaccgta ctggcgttac tggcctgtgg ttttgcctgc ttcattgaga tgggaaaaat
   113281 tccctttgat gttgctgaag cagaacagga attacaggaa ggcccgctga ccgaatattc
   113341 cggtgccggg ctggcgctag cgaaatgggg gctggggctg aaacaggtcg tgatggcagc
   113401 actgtttgtg gccctgtttc tgccctttgg gcgcgcgcaa gaactttctc tcgcctgcct
   113461 gctgacttca cttgtcgtta cgctgctcaa ggttttgctg atttttgtac tggcctcaat
   113521 cgcagaaaac acgctggcac gcgggcgttt tttactcatt caccatgtga cctggcttgg
   113581 cttcagcctt gctgcgcttg catgggtctt ttggttaacc ggtctgtaag gagcactgac
   113641 ggaatatgga aaatcttgct ctgacgacgt taatgctgcc ttttatcggc gcactggtcg
   113701 tttcgttttc gccacaacgt cgggccgccg aatggggggt tttgttcgcc gcgctgacca
   113761 cgctgtgcat gttgtcgctg atctccgcgt tttatcaggc cgataaagtt gccgtcacgt
   113821 tgacgttggt caacgtgggg gatgtggcat tatttggcct ggtcattgat cgcgtgagta
   113881 cgctgattct gtttgtggtg gtgtttctcg gtttgctggt cacgatctac tccacgggtt
   113941 atctgacgga taaaaatcgc gaacacccgc ataacggcac gaatcgttat tacgcatttt
   114001 tgctggtgtt tatcggcgcg atggcgggac tggtactctc ctcgacgctg ctcggtcagt
   114061 tgttgttttt tgaaattacg ggcggctgct cctgggcgtt gatcagttat taccagagcg
   114121 ataaagcgca gcgttcagca ctaaaagcgt tgcttatcac ccatatcggt tcgttggggt
   114181 tgtatcttgc cgccgccacg ctgtttttgc agaccggaac gtttgtgctt agcgcgatga
   114241 gcgagttaca tggcgacgca cgttatctgg tttatggcgg catcctgttt gccgcgtggg
   114301 ggaaatcggc ccagctaccg atgcaagcgt ggctaccgga cgcaatggaa gcgccaacac
   114361 cgatcagcgc ctatctccac gccgcatcga tggtgaaagt gggcgtttac atttttgccc
   114421 gcgccattat cgacggcggc aatatcccgc atgtgattgg cggcgttggc atggtcatga
   114481 cactggtcac cattctttat ggctttctga tgtatttgcc acagcaggat atgaagcggt
   114541 tgctggcctg gtcgaccatc actcaacttg gctggatgtt cttcggcttg tcgctctcca
   114601 tcttcggctc gcggctggcg ctggagggta gcatcgccta catcgtcaac cacgcgttcg
   114661 ctaaaagcct gtttttcctt gtagcaggtg cgctgagtta cagctgcggc acgcgcttgt
   114721 tgccgcgtct gcgtggcgta ttgcacaccc tgccgttgcc aggcgtgggt ttctgcgtgg
   114781 cagcgctggc aattaccggt gtgccgccgt tcaacggctt cttcagtaaa ttcccgctgt
   114841 ttgccgccgg ttttgcgttg tcagtggagt actggatcct gctgcccgcc atgattctgc
   114901 tgatgattga atcggtcgcc agtttcgcct ggtttattcg ctggtttggt cgcgtcgtgc
   114961 cgggcaaacc gagcgaggcc gtcgccgatg ccgcaccgct gccagggtca atgcgcctgg
   115021 tgttgattgt actgattgtg atgtcgctga tttccagcgt aatcgccgcg acctggttgc
   115081 agtaaggaga tgatgaatga ccggttctat gatcgtaaat aatctggcgg gactgatgat
   115141 gctgacatcg ctgtttgtga ttagcgtcaa aagctatcgc ctgtcatgcg gattttacgc
   115201 ctgccagtcg ctggtgctgg tgtctatttt cgccactctc tcgtgcctgt tcgccacaga
   115261 gcaactgctg atctggtccg ccagcgcctt tatcaccaaa gtgctgctgg taccgttaat
   115321 catgacttac gctgcacgaa atattcccca gaacatcccg gaaaaagcgt tattcggtcc
   115381 ggcaatgatg gcactgctcg cggcgttaat tgtcctgctt tgcgcatttg tcgttcagcc
   115441 cgtgaagcta ccgatggcta ccgggctgaa accggcgctg gcggtagcgt taggtcattt
   115501 tctgcttggc ctgctgtgca ttgtcagcca acgcaatatc ctgcggcaaa tttttggtta
   115561 ctgcctgatg gaaaacggct cccatctggt gctggcgctt cttgcctggc gagcaccgga
   115621 actggtggaa ataggtatcg ctaccgacgc catcttcgcc gtcattgtga tggtgttact
   115681 ggcaagaaaa atatggcgta cccacggcac gctggacgtg aacaacttga ccgcgctgaa
   115741 gggataatga gatgagttat tctgtgatgt tcgctttact cctgctcacg ccgctgcttt
   115801 tttcgctgct ctgttttgcc tgccggaaac gggggctctc tgcgacttgt acggtgactg
   115861 tattacatag cttagggatc acactgctgc tgattctagc actctgggtg gtccaaactg
   115921 ccgctgatgc aggagaaata ttcgctgcgg gactgtggct tcatattgat ggtctgggcg
   115981 gtttgttcct cgccattctt ggtgtgattg gctttctcac cggtgtttac tcgattggct
   116041 acatgcgtca tgaagtggca cacggcgagc tttcacccgt tacactgtgc gattactacg
   116101 gtttcttcca tctgtttttg ttcaccatgc tgctggttgt taccagcaat aacctgattg
   116161 tgatgtgggc ggcgatcgaa gccaccacct taagctcggc gtttctggta ggcatttacg
   116221 gtcagcgttc atcgctggaa tcagcatgga agtacatcat tatttgtact gttggtgtcg
   116281 cttttggtct gttcggtacc gtgctggtat acgccaacgc cgccagcgtt atgccgcagg
   116341 cagaaatggc gatattctgg agcgaggttc ttaagcaatc gtccttgctt gacccaacat
   116401 taatgctgtt ggcctttgtg tttgtgctaa ttggctttgg taccaaaacc gggctatttc
   116461 ccatgcacgc ctggctgccg gatgctcaca gtgaagcgcc gagtccggtc agcgccctgc
   116521 tctccgccgt attgctgaac tgcgcgctgt tggtgctgat tcgctattac atcattattt
   116581 gccaggccat cggcagcgat ttccccaacc ggttgttgct catcttcggc atgttgtcgg
   116641 ttgccgtggc ggcatttttc attctggtac agcgggacat taagcgtctg ctggcgtact
   116701 ccagcgtgga gaacatgggg ctggtcgcgg tggcgctagg cattggcggg ccgctgggaa
   116761 tttttgccgc gctgctgcac accttaaacc acagtctggc aaaaacgctg ctgttctgcg
   116821 gttccggcaa tgtactgctc aagtacggca cgcgcgatct caacgtcgtc tgtgggatgc
   116881 tcaaaatcat gccatttacc gccgtgctgt ttggcggcgg tgcgctggcg ctggcgctgg
   116941 cagggatgcc gcccttcaac atttttctta gcgaatttat gaccgttacc gcaggactgg
   117001 cacgtaatca cctgctgatt atcgtcctgc tgttattgct gttaacgctg gtgctggcgg
   117061 gcctggtacg gatggctgcg cgggtgttaa tggcgaaacc gccgcaggcc gttaaccggg
   117121 gtgatctcgg ctggttgacc acctcgccaa tggtgattct gctggtcatg atgctggcga
   117181 tgggaacgca tattccacaa cctgtcatca ggatcctggc gggcgcttcc actatagtcc
   117241 tctcagggac gcacgacctg cctgcacaac gtagcacctg gcatgatttt ttgccttcag
   117301 gcaccgcatc tgtttcggag aaacacagtg aacgttaatt catcgtcaaa tcgtggcgaa
   117361 gcgattctcg ccgccctgaa aacgcagttc cccggcgcgg tgctggatga agagcgacaa
   117421 acgcctgaac aggtcaccat tacggtgaaa atcaatctgc tgcctgacgt tgtacattat
   117481 ctttattatc aacatgatgg ctggcttcca gtcctgtttg gcaacgacga gcggacactt
   117541 aacggtcatt acgcggttta ttatgccctt tcaatggaag gggccgaaaa atgctggatc
   117601 gtggtgaagg cactggtcga tgccgacagt cgggagtttc cgtcagtcac accgcgcgtc
   117661 cctgccgcgg tctggggcga gcgagaaatt cgcgatatgt acgggctgat tccggttggc
   117721 ctgccggatc agcgtcgcct ggtgttgccc gatgactggc cggaagatat gcatccgctg
   117781 cgcaaagatg cgatggatta tcgactgcgc cctgaaccga cgacagattc cgaaacgtat
   117841 gcgtttatca atgagggcaa cagcgatgcg cgggtgatcc ctgtcggccc gctgcatatc
   117901 acctcctatg aaccgggtca cttccgcttg tttgtggatg gcgagcaaat tgtcgatgct
   117961 gattaccgcc tgttttatgt ccatcgcggc atggagaaac tggcagaaac gcggatgggc
   118021 tacaacgaag tgaccttctt atcggaccgc gtgtgtggga tttgcggttt tgcccacagt
   118081 gtggcctata ccaactcggt tgaaaatgca ctggggattg aggtgccgca acgagcacat
   118141 actattcgct cgattctgct ggaagtcgag cggctacaca gtcatttgct caaccttggc
   118201 ctctcctgcc attttgttgg ttttgatacc ggctttatgc aatttttccg cgtgcgggaa
   118261 aagtcgatga cgatggcgga attgctgacc gggtcgcgta aaacctacgg tctgaatctg
   118321 attggtggtg ttcgccgcga tattctcaaa gagcaacgtc tgcaaacgct gaaactggtg
   118381 cgcgagatgc gcgccgacgt gtcggagctg gtagagatgc tgcttgctac gccgaatatg
   118441 gaacaacgca ctcagggcat tggcattctc gaccgacaaa tcgcccgtga ttatagccct
   118501 gtagggccgc tgatccgcgc agtggttttg cccgtgattt gcgctttgat cacccctacg
   118561 ccgactacgg caatattcca aaaacactgt ttacctttac cggcggcgat gttttctccc
   118621 gcgtgatggt ccgtgtcaaa gagacgtttg attcgctggc aatgctggaa tttgccctcg
   118681 acaacatgcc ggatacccca ctgctgaccg aaggctttag ctataaacct cacgcattcg
   118741 cgctgggctt tgttgaagcg ccacgcggtg aagacgtgca ctggagcatg ctcggtgata
   118801 accaaaaatt gttccgctgg cgctgtcgtg ccgccaccta cgccaactgg ccggtgttgc
   118861 gttacatgct gcgcggcaat accgtttctg acgcaccgct gattatcggt agccttgatc
   118921 cctgctactc ctgtaccgac cgtgtgacgc tggtagatgt gcgcaagcgc cagtcaaaaa
   118981 ccgtgctgta taaagagatc gaacgctacg gcattgatcg taaccgttcg ccgctgaagt
   119041 aaggacagaa gatgctgaag ttactgaaaa ctattatgcg cgccggaacc gcgacggtga
   119101 aatatccctt cgcgccactg gaggtcagcc caggctttcg cgggaaaccg gacctgatgc
   119161 ccagccaatg tattgcctgc ggtgcctgcg cctgtgcttg tccggcaaat gcgctgacta
   119221 tccagaccga cgaccagcaa aattcgcgca cctggcagct ctatctgggg cgttgtattt
   119281 actgcggacg ttgtgaagaa gtgtgcccga ccagagccat ccagcttacc aataactttg
   119341 aactgaccgt cacccataaa gccgatctct atacccacgc gacgttccat ctacaacgtt
   119401 gcagccgttg cgaacgcccg tttgccccgc aaaaaaccgt cgcactggct gctgaattgt
   119461 tggcacagca acaaaatgct ccacaaaacc gcgaaatgtt atgggcgcaa gcgagcgtct
   119521 gcccggagtg caaacaacgc gcaacgctga tcaacgacga tacagatgta ccgctggtgg
   119581 ctaaggagca gctatgagtc cagtgcttac gcaatatgtc agccagccca tcacgctgga
   119641 cgagcaaacg caaaagatga agcggcattt gctacaggat atccgtcgct cggcttacgt
   119701 ttatcgcatc gattgcggcg gctgcaacgc ctgtgaaatc gaaatttttg ctgccatcac
   119761 ccccgtattc gacgcagaac gttttggcat taaggttgtt tcatcaccgc gtcacgccga
   119821 tattttgtta tttactggcg cagtcacccg ggcgatgcgt atgcctgcac ttcgggcgta
   119881 tgagtctgcc cccgatcata aaatctgtgt ttcctacggc gcgtgcggtg tcggcggcgg
   119941 tattttccac gatctctaca gcgtctgggg cggcagcgac accattgtcc ccattgatgt
   120001 ttggatcccc ggctgcccgc caacaccggc cgccaccatt cacggtttcg ccgtggcgct
   120061 cggtttgctg caacagaaga ttcacgcggt ggattatcgc gatcccaccg gggtgactat
   120121 gcaaccgttg tggccgcaga tcccgccatc acagcgtatc gccattgagc gagaagcgcg
   120181 gcggctggcg ggctatcgtc aggggcgaga aatttgcgat cggctcctgc gccatttaag
   120241 cgacgatcct acaggaaatc gggttaacac ctggttgcgc gatgccgacg atccacgtct
   120301 caatagtatc gttcagcaac tctttcgcgt actccggggg ttacatgact gaagagtgcg
   120361 gggaaattgt tttctggacg ctgcgaaaaa agtttgtcgc cagtagcgac gagatgccgg
   120421 aacacagctc tcaggtaatg tattactcgc tggctatcgg tcatcacgtt ggcgtgattg
   120481 attgtctgaa tgtcgccttc cgctgcccac tgacggaata cgaagattgg cttgcactgg
   120541 tcgaagagga gcaagcccga cgtaagatgc tgggggtgat gacttttggt gagattgtta
   120601 ttgacgccag ccacaccgcc ctgttgaccc gggcattcgc gccactggca gatgacgcga
   120661 cgtctgtgtg gcaagcgcgt agcattcaat tcattcatct gttggatgaa attgtgctgg
   120721 aaccggctat ctatctgatg gccagaaaaa ttgcgtgaga aggatttctc attaataagg
   120781 actgttgatg gctatgtcag acgaagcgat gtttgccccg ccgcaaggaa taacaattga
   120841 agcggtaaac ggaatgctcg cggagcggtt agcgcagaaa cacggcaagg cgtctttatt
   120901 acgcgccttc atcccgctgc cgccgccgtt cagcccggta caacttattg aactgcatgt
   120961 tctcaaaagc aacttctatt accgctacca tgatgatggc agcgatgtga cggcaacaac
   121021 agagtatcag ggcgagatgg tcgattattc gcgttacgcc gtccttctcg gcagtagtgg
   121081 aatggcggag ctacgcttta ttcgtaccca cggcagccgt tttactcccc aggattgcac
   121141 actgtttaac tggctggcac gcattatcac cccggttctg caatcatggc tcaatgatga
   121201 agcacagcag gtggcgctgc gtttgctgga gaaagatcgc gatcatcatc gggtactggt
   121261 ggatatcact aatgcagtgc tgtcacatct tgatctcgac gatctgatcg ctgacgtcgc
   121321 tcgtgagatc catcattttt tcggtctggc ttcagtcagt atggtactgg gcgatcatcg
   121381 aaagaacgag aagttcagcc tgtggtgcag cgatctttct gcctcacatt gtgcgtgtct
   121441 gccacacaat atgcctggcg acagtgtatt gctgacacaa acgctacaaa cccgacaacc
   121501 gaccttgacg caccgtgcag atgatctgtt tctctggcaa cgcgacccgt tattactctt
   121561 acttgcatct aacggctgcg aatctgcgct ccttataccg cttacctttg gcaaccatac
   121621 accgggtgca ttgttgctgg cgcatacctc ttccactctc tttagtgagg aaaactgcca
   121681 gctactacaa cacatagccg atcgcatcgc tattgccgtt ggcaatgccg atgcctgccg
   121741 tagaatgacc gatttgcagg aaagtttgca gcaagaaaac caccagctta gcgagcagct
   121801 cctttcgaat ctgggcgtcg gtgacattat ctatcaaagc caggcaatgg aagacctgct
   121861 ccagcaggta gatattgtgg cgaagagcga cagtacggtg ttgatttgcg gtgaaaccgg
   121921 aaccggcaaa gaggtgatcg ccagagcgat ccatcaactt agcccgcgac acgacaagcc
   121981 gctggtcaaa atcaactgcg ctgccatccc cgccagtctt ctggaaagtg agttattcgg
   122041 tcatgacaaa ggggcgttta ctggtgcgat taatacccat cgtggtcgtt ttgaaattgc
   122101 cgatggcggc acgttgtttc tcgatgaaat tggcgatctg ccgttagaac ttcagcctaa
   122161 actgctgcgc gtattgcagg aacgggagat tgagcgtctc ggcgggagta gaacgatccc
   122221 ggtaaatgtc agagtcattg ccgccaccaa ccgtgatttg tggcaaatgg ttgaagatcg
   122281 ccagtttcgc agcgatctct tttatcgcct gaatgtcttc ccactggaat tgcctccgct
   122341 gcgcgaccgt ccggaagata tccctctttt agcaaagcat ttcacgcaaa aaatggcgcg
   122401 ccatatgaat cgttcaattg acgccatccc gaccgaggcg ctacgccagt tgatgtcgtg
   122461 ggactggccg ggcaacgtgc gcgagctgga aaacgtgatt gagcgagcgg tactgctgac
   122521 tcgcggtaac agtctgaatt tacatcttaa tgtccgacaa agccgtttac tgccgacgct
   122581 aaatgaagat tcagcgcttc gcagttcaat ggcgtagttg ctgcacccga cgacgccaga
   122641 gaatgacgaa gaagaacgtc agcgcattgt tcaggtattg cgagaaacca atggcattgt
   122701 tgccgggccc cgtggcgcgg cgacacgatt agggatgaag cgcaccacgc tgctgtcacg
   122761 aatgcagcgt ctgggaatct cggttcgcga ggtgttgtaa tctgcttttg caggagtatg
   122821 catgagaaac aaactctctt tcgacttgca gttgagcgcc agaaaagcgg caatcgctga
   122881 acggattgcc gcccataaaa ttgcccgcag taaagtgtcg gtctttttaa tggcgatgtc
   122941 cgctggcgtg tttatggcga tgtccgctgg cgtgtttatg gcgatcggat ttacttttta
   123001 cctttccgtt atcgccgatg ccccgtcttc ataggcatta acccatctgg tgggcggcct
   123061 ttgctttaca cttggcttta ttttgctggc ggtttgcggc accagcctgt tcacctcgtc
   123121 ggtaatgacg gtaatggcaa aaagtcgggg cgttattagt tggcgaactt ggctgattaa
   123181 cgcacttctg gtggcctgcg gtaatctggc aggtattgcc tgtttcagtt tgttaatctg
   123241 gttttccggg ctggtgatga gtgaaaacgc gatgtgggga gtcgcgattt tacactgcgc
   123301 cgagggcaaa atgcatcata catttactga atctgtcagc ctcggcatta tgtgcaatct
   123361 gatggtttgc ctggcgctgt ggatgagtta ttgcggacgt tcgttatgcg acaaaatcgt
   123421 cgccatgatt ttgtccatca ccctgtttgt cgccagtggc tttgagcact gtatcgccaa
   123481 tttgtttgtg attccgttcg ccattgccat tcgccatttc gcccctacct ccttctggca
   123541 actggcgcac agtagcgcag acaattttcc ggcactgacg gtcagccatt ttattaccgc
   123601 caatctgctc ccggtgatgc tgggtaatat tatcggcggt gcagtgctgg tgagtatttg
   123661 tt
//
LOCUS       DAJTUI010000002        95082 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTUI010000002 DAJTUI010000000
VERSION     DAJTUI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.002"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(89..1339)
                     /gene="icd"
                     /locus_tag="OO464_000121"
     CDS             complement(89..1339)
                     /gene="icd"
                     /locus_tag="OO464_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6225421.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1511..2164
                     /gene="rluE"
                     /locus_tag="OO464_000122"
     CDS             1511..2164
                     /gene="rluE"
                     /locus_tag="OO464_000122"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6225422.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2174..2635
                     /gene="nudJ"
                     /locus_tag="OO464_000123"
     CDS             2174..2635
                     /gene="nudJ"
                     /locus_tag="OO464_000123"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6225423.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2689..3795
                     /gene="mnmA"
                     /locus_tag="OO464_000124"
     CDS             2689..3795
                     /gene="mnmA"
                     /locus_tag="OO464_000124"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6225424.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3831..4472
                     /gene="hflD"
                     /locus_tag="OO464_000125"
     CDS             3831..4472
                     /gene="hflD"
                     /locus_tag="OO464_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6225425.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4476..5846
                     /gene="purB"
                     /locus_tag="OO464_000126"
     CDS             4476..5846
                     /gene="purB"
                     /locus_tag="OO464_000126"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6225426.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6015..6686
                     /gene="phoP"
                     /locus_tag="OO464_000127"
     CDS             6015..6686
                     /gene="phoP"
                     /locus_tag="OO464_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6225427.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6686..8146
                     /gene="phoQ"
                     /locus_tag="OO464_000128"
     CDS             6686..8146
                     /gene="phoQ"
                     /locus_tag="OO464_000128"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6225428.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8943..9224)
                     /locus_tag="OO464_000129"
     CDS             complement(8943..9224)
                     /locus_tag="OO464_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225429.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9238..10899)
                     /locus_tag="OO464_000130"
     CDS             complement(9238..10899)
                     /locus_tag="OO464_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6225430.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10883..11209)
                     /locus_tag="OO464_000131"
     CDS             complement(10883..11209)
                     /locus_tag="OO464_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6225431.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11265..11447)
                     /locus_tag="OO464_000132"
     CDS             complement(11265..11447)
                     /locus_tag="OO464_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225432.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11431..11871)
                     /locus_tag="OO464_000133"
     CDS             complement(11431..11871)
                     /locus_tag="OO464_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6225433.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11871..12167)
                     /locus_tag="OO464_000134"
     CDS             complement(11871..12167)
                     /locus_tag="OO464_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR6225434.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12164..12502)
                     /locus_tag="OO464_000135"
     CDS             complement(12164..12502)
                     /locus_tag="OO464_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6225435.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12499..13710)
                     /locus_tag="OO464_000136"
     CDS             complement(12499..13710)
                     /locus_tag="OO464_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6225436.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13712..14284)
                     /locus_tag="OO464_000137"
     CDS             complement(13712..14284)
                     /locus_tag="OO464_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR6225437.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14324..15481)
                     /locus_tag="OO464_000138"
                     /pseudo
     CDS             complement(14324..15481)
                     /locus_tag="OO464_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15785..15946)
                     /locus_tag="OO464_000139"
     CDS             complement(15785..15946)
                     /locus_tag="OO464_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6225438.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(15956..16261)
                     /locus_tag="OO464_000140"
     CDS             complement(15956..16261)
                     /locus_tag="OO464_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225439.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16258..16536)
                     /locus_tag="OO464_000141"
     CDS             complement(16258..16536)
                     /locus_tag="OO464_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225440.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(16825..18573)
                     /locus_tag="OO464_000142"
     CDS             complement(16825..18573)
                     /locus_tag="OO464_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6225441.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18570..18869)
                     /locus_tag="OO464_000143"
     CDS             complement(18570..18869)
                     /locus_tag="OO464_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6225442.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(18875..19108)
                     /locus_tag="OO464_000144"
     CDS             complement(18875..19108)
                     /locus_tag="OO464_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225443.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19110..19330)
                     /locus_tag="OO464_000145"
                     /pseudo
     CDS             complement(19110..19330)
                     /locus_tag="OO464_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19323..19544)
                     /locus_tag="OO464_000146"
     CDS             complement(19323..19544)
                     /locus_tag="OO464_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225444.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19534..19734)
                     /locus_tag="OO464_000147"
     CDS             complement(19534..19734)
                     /locus_tag="OO464_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6225445.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            19864..20043
                     /locus_tag="OO464_000148"
     CDS             19864..20043
                     /locus_tag="OO464_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225446.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20168..20497
                     /locus_tag="OO464_000149"
     CDS             20168..20497
                     /locus_tag="OO464_000149"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225447.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20547..20735)
                     /locus_tag="OO464_000150"
     CDS             complement(20547..20735)
                     /locus_tag="OO464_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6225448.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21110..22339)
                     /locus_tag="OO464_000151"
     CDS             complement(21110..22339)
                     /locus_tag="OO464_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6225449.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22588..23709
                     /gene="roxA"
                     /locus_tag="OO464_000152"
                     /gene_synonym="ycfD"
     CDS             22588..23709
                     /gene="roxA"
                     /locus_tag="OO464_000152"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6225450.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(23758..24984)
                     /gene="pepT"
                     /locus_tag="OO464_000153"
     CDS             complement(23758..24984)
                     /gene="pepT"
                     /locus_tag="OO464_000153"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6225451.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25234..26370
                     /gene="potA"
                     /locus_tag="OO464_000154"
     CDS             25234..26370
                     /gene="potA"
                     /locus_tag="OO464_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR6225452.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26354..27211
                     /gene="potB"
                     /locus_tag="OO464_000155"
     CDS             26354..27211
                     /gene="potB"
                     /locus_tag="OO464_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR6225453.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27208..28002
                     /gene="potC"
                     /locus_tag="OO464_000156"
     CDS             27208..28002
                     /gene="potC"
                     /locus_tag="OO464_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6225454.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            27999..29045
                     /gene="potD"
                     /locus_tag="OO464_000157"
     CDS             27999..29045
                     /gene="potD"
                     /locus_tag="OO464_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6225455.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29201..30022)
                     /gene="cobB"
                     /locus_tag="OO464_000158"
     CDS             complement(29201..30022)
                     /gene="cobB"
                     /locus_tag="OO464_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6225456.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30038..30949)
                     /gene="nagK"
                     /locus_tag="OO464_000159"
     CDS             complement(30038..30949)
                     /gene="nagK"
                     /locus_tag="OO464_000159"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6225457.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(30978..32222)
                     /gene="lolE"
                     /locus_tag="OO464_000160"
     CDS             complement(30978..32222)
                     /gene="lolE"
                     /locus_tag="OO464_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6225458.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32222..32923)
                     /gene="lolD"
                     /locus_tag="OO464_000161"
     CDS             complement(32222..32923)
                     /gene="lolD"
                     /locus_tag="OO464_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6225459.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(32916..34115)
                     /gene="lolC"
                     /locus_tag="OO464_000162"
     CDS             complement(32916..34115)
                     /gene="lolC"
                     /locus_tag="OO464_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6225460.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34377..35450
                     /locus_tag="OO464_000163"
     CDS             34377..35450
                     /locus_tag="OO464_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6225461.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35578..39024
                     /gene="mfd"
                     /locus_tag="OO464_000164"
     CDS             35578..39024
                     /gene="mfd"
                     /locus_tag="OO464_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6225462.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39167..40129
                     /gene="ldtC"
                     /locus_tag="OO464_000165"
     CDS             39167..40129
                     /gene="ldtC"
                     /locus_tag="OO464_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR6225463.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40212..40424)
                     /locus_tag="OO464_000166"
     CDS             complement(40212..40424)
                     /locus_tag="OO464_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6225464.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            40709..41341
                     /gene="comR"
                     /locus_tag="OO464_000167"
     CDS             40709..41341
                     /gene="comR"
                     /locus_tag="OO464_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6225465.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41403..41942)
                     /locus_tag="OO464_000168"
     CDS             complement(41403..41942)
                     /locus_tag="OO464_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6225466.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42169..43473)
                     /gene="ndh"
                     /locus_tag="OO464_000169"
     CDS             complement(42169..43473)
                     /gene="ndh"
                     /locus_tag="OO464_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6225467.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(43881..44423)
                     /locus_tag="OO464_000170"
     CDS             complement(43881..44423)
                     /locus_tag="OO464_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6225468.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44446..45471)
                     /gene="nagZ"
                     /locus_tag="OO464_000171"
     CDS             complement(44446..45471)
                     /gene="nagZ"
                     /locus_tag="OO464_000171"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6225469.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45482..46306)
                     /gene="thiK"
                     /locus_tag="OO464_000172"
     CDS             complement(45482..46306)
                     /gene="thiK"
                     /locus_tag="OO464_000172"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6225470.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46287..46928)
                     /gene="lpoB"
                     /locus_tag="OO464_000173"
     CDS             complement(46287..46928)
                     /gene="lpoB"
                     /locus_tag="OO464_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6225471.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(46943..47320)
                     /locus_tag="OO464_000174"
     CDS             complement(46943..47320)
                     /locus_tag="OO464_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6225472.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47323..47682)
                     /gene="hinT"
                     /locus_tag="OO464_000175"
     CDS             complement(47323..47682)
                     /gene="hinT"
                     /locus_tag="OO464_000175"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6225473.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48015..50200
                     /gene="fhuE"
                     /locus_tag="OO464_000176"
                     /pseudo
     CDS             48015..50200
                     /gene="fhuE"
                     /locus_tag="OO464_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50260..51693)
                     /gene="ptsG"
                     /locus_tag="OO464_000177"
     CDS             complement(50260..51693)
                     /gene="ptsG"
                     /locus_tag="OO464_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6225474.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(51988..52785)
                     /locus_tag="OO464_000178"
     CDS             complement(51988..52785)
                     /locus_tag="OO464_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6225475.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(52796..53800)
                     /gene="holB"
                     /locus_tag="OO464_000179"
     CDS             complement(52796..53800)
                     /gene="holB"
                     /locus_tag="OO464_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6225476.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(53797..54438)
                     /locus_tag="OO464_000180"
     CDS             complement(53797..54438)
                     /locus_tag="OO464_000180"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6225477.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54428..55450)
                     /gene="yceG"
                     /locus_tag="OO464_000181"
     CDS             complement(54428..55450)
                     /gene="yceG"
                     /locus_tag="OO464_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6225478.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55453..56262)
                     /gene="pabC"
                     /locus_tag="OO464_000182"
     CDS             complement(55453..56262)
                     /gene="pabC"
                     /locus_tag="OO464_000182"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6225479.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56382..57623)
                     /gene="fabF"
                     /locus_tag="OO464_000183"
     CDS             complement(56382..57623)
                     /gene="fabF"
                     /locus_tag="OO464_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6225480.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(57710..57946)
                     /gene="acpP"
                     /locus_tag="OO464_000184"
     CDS             complement(57710..57946)
                     /gene="acpP"
                     /locus_tag="OO464_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6225481.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58157..58891)
                     /gene="fabG"
                     /locus_tag="OO464_000185"
     CDS             complement(58157..58891)
                     /gene="fabG"
                     /locus_tag="OO464_000185"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6225482.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(58904..59833)
                     /gene="fabD"
                     /locus_tag="OO464_000186"
     CDS             complement(58904..59833)
                     /gene="fabD"
                     /locus_tag="OO464_000186"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6225483.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(59849..60802)
                     /gene="fabH"
                     /locus_tag="OO464_000187"
     CDS             complement(59849..60802)
                     /gene="fabH"
                     /locus_tag="OO464_000187"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6225484.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(60870..61940)
                     /gene="plsX"
                     /locus_tag="OO464_000188"
     CDS             complement(60870..61940)
                     /gene="plsX"
                     /locus_tag="OO464_000188"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6225485.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62021..62194)
                     /gene="rpmF"
                     /locus_tag="OO464_000189"
     CDS             complement(62021..62194)
                     /gene="rpmF"
                     /locus_tag="OO464_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6225486.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62246..62767)
                     /gene="yceD"
                     /locus_tag="OO464_000190"
     CDS             complement(62246..62767)
                     /gene="yceD"
                     /locus_tag="OO464_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6225487.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            62966..63550
                     /gene="yceF"
                     /locus_tag="OO464_000191"
     CDS             62966..63550
                     /gene="yceF"
                     /locus_tag="OO464_000191"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6225488.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(63662..64621)
                     /gene="rluC"
                     /locus_tag="OO464_000192"
     CDS             complement(63662..64621)
                     /gene="rluC"
                     /locus_tag="OO464_000192"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6225489.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65194..68385
                     /gene="rne"
                     /locus_tag="OO464_000193"
     CDS             65194..68385
                     /gene="rne"
                     /locus_tag="OO464_000193"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6225490.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68582..69524)
                     /gene="flgL"
                     /locus_tag="OO464_000194"
                     /pseudo
     CDS             complement(68582..69524)
                     /gene="flgL"
                     /locus_tag="OO464_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69536..71178)
                     /gene="flgK"
                     /locus_tag="OO464_000195"
                     /pseudo
     CDS             complement(69536..71178)
                     /gene="flgK"
                     /locus_tag="OO464_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71244..72185)
                     /gene="flgJ"
                     /locus_tag="OO464_000196"
     CDS             complement(71244..72185)
                     /gene="flgJ"
                     /locus_tag="OO464_000196"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR6225491.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72185..73282)
                     /gene="flgI"
                     /locus_tag="OO464_000197"
     CDS             complement(72185..73282)
                     /gene="flgI"
                     /locus_tag="OO464_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6225492.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73294..73992)
                     /gene="flgH"
                     /locus_tag="OO464_000198"
     CDS             complement(73294..73992)
                     /gene="flgH"
                     /locus_tag="OO464_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6225493.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74045..74827)
                     /gene="flgG"
                     /locus_tag="OO464_000199"
     CDS             complement(74045..74827)
                     /gene="flgG"
                     /locus_tag="OO464_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR6225494.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(74965..75716)
                     /gene="flgF"
                     /locus_tag="OO464_000200"
                     /pseudo
     CDS             complement(74965..75716)
                     /gene="flgF"
                     /locus_tag="OO464_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(75736..76875)
                     /locus_tag="OO464_000201"
     CDS             complement(75736..76875)
                     /locus_tag="OO464_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6225495.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(76900..77595)
                     /gene="flgD"
                     /locus_tag="OO464_000202"
     CDS             complement(76900..77595)
                     /gene="flgD"
                     /locus_tag="OO464_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR6225496.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(77607..78010)
                     /gene="flgC"
                     /locus_tag="OO464_000203"
                     /pseudo
     CDS             complement(77607..78010)
                     /gene="flgC"
                     /locus_tag="OO464_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78014..78430)
                     /gene="flgB"
                     /locus_tag="OO464_000204"
     CDS             complement(78014..78430)
                     /gene="flgB"
                     /locus_tag="OO464_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR6225497.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            78585..79244
                     /gene="flgA"
                     /locus_tag="OO464_000205"
     CDS             78585..79244
                     /gene="flgA"
                     /locus_tag="OO464_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6225498.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79320..79613
                     /gene="flgM"
                     /locus_tag="OO464_000206"
     CDS             79320..79613
                     /gene="flgM"
                     /locus_tag="OO464_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR6225499.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            79618..80034
                     /gene="flgN"
                     /locus_tag="OO464_000207"
     CDS             79618..80034
                     /gene="flgN"
                     /locus_tag="OO464_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6225500.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80074..81609)
                     /gene="murJ"
                     /locus_tag="OO464_000208"
     CDS             complement(80074..81609)
                     /gene="murJ"
                     /locus_tag="OO464_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6225501.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(81809..82642)
                     /locus_tag="OO464_000209"
     CDS             complement(81809..82642)
                     /locus_tag="OO464_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6225502.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLCD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(82644..83291)
                     /locus_tag="OO464_000210"
     CDS             complement(82644..83291)
                     /locus_tag="OO464_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR6225503.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83302..83886)
                     /gene="rimJ"
                     /locus_tag="OO464_000211"
     CDS             complement(83302..83886)
                     /gene="rimJ"
                     /locus_tag="OO464_000211"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6225504.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84122..85330
                     /gene="mdtH"
                     /locus_tag="OO464_000212"
     CDS             84122..85330
                     /gene="mdtH"
                     /locus_tag="OO464_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6225505.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85394..86041
                     /gene="grxB"
                     /locus_tag="OO464_000213"
     CDS             85394..86041
                     /gene="grxB"
                     /locus_tag="OO464_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6225506.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86175..86735
                     /locus_tag="OO464_000214"
     CDS             86175..86735
                     /locus_tag="OO464_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6225507.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            86841..87887
                     /gene="pyrC"
                     /locus_tag="OO464_000215"
     CDS             86841..87887
                     /gene="pyrC"
                     /locus_tag="OO464_000215"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6225508.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            87961..88206
                     /gene="dinI"
                     /locus_tag="OO464_000216"
     CDS             87961..88206
                     /gene="dinI"
                     /locus_tag="OO464_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6225509.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88496..88750
                     /gene="bssS"
                     /locus_tag="OO464_000217"
     CDS             88496..88750
                     /gene="bssS"
                     /locus_tag="OO464_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6225510.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            88865..89983
                     /gene="solA"
                     /locus_tag="OO464_000218"
     CDS             88865..89983
                     /gene="solA"
                     /locus_tag="OO464_000218"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6225511.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90031..90144
                     /locus_tag="OO464_000219"
     CDS             90031..90144
                     /locus_tag="OO464_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR6225512.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90405..90971
                     /locus_tag="OO464_000220"
     CDS             90405..90971
                     /locus_tag="OO464_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6225513.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            90975..91550
                     /locus_tag="OO464_000221"
     CDS             90975..91550
                     /locus_tag="OO464_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6225514.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(91592..92644)
                     /locus_tag="OO464_000222"
     CDS             complement(91592..92644)
                     /locus_tag="OO464_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6225515.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            92869..93789
                     /gene="lpxL"
                     /locus_tag="OO464_000223"
     CDS             92869..93789
                     /gene="lpxL"
                     /locus_tag="OO464_000223"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6225516.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            93961..94158
                     /locus_tag="OO464_000224"
     CDS             93961..94158
                     /locus_tag="OO464_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR6225517.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94182..>95082
                     /locus_tag="OO464_000225"
     CDS             94182..>95082
                     /locus_tag="OO464_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6225518.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFR"
BASE COUNT        22880 a        25208 c        23472 g        23522 t
ORIGIN      
        1 atatgctcga tatgctatta ataatatcca cataaataaa acaacgggcg tgttatacgc
       61 ccgtttaaat atttaacaca tgtagtgatt acatattctt gatgatcgca tcaccaaact
      121 ctgaacattt cagcagttta gcgccttcca tcagacgctc gaagtcatag gttacggtct
      181 tggcgttgat cgcgccttcc atacctttaa caatcaggtc tgcggcttca gtccagccca
      241 tatggcgtaa catcatctca gcggagagaa taatagagcc agggtttact ttgtcctgac
      301 cggcgtattt cggcgcagta ccgtgggtgg cttcaaacag ggcgcattcg tcaccgatgt
      361 ttgctccagg ggcgataccg ataccgccaa cctgcgctgc cagagcgtca gaaatgtagt
      421 caccgttcag gttcatacag gcgataacat catattcagc cggacgcagc aggatctgtt
      481 gcaggaatgc atcagcaatc acgtctttaa tgacgatctc tttgccggta ttcgggtttt
      541 taactttcag ccacgggccg ccgtcgatca gttcaccgcc aaattcttca cgcgccagct
      601 ggtagcccca gtctttaaac gcgccttcgg tgaacttcat gatgttgcct ttgtgcacca
      661 gggtcacaga gtcacgatcg ttagcaattg cgtattcgat cgctgcacga accagacgtt
      721 tggtgccttc ttcagaacac ggcttaatac cgataccgca atgttccggg aagcgaattt
      781 tcttcacgcc catctcttca cgcaggaatt taatcacttt ctcggcgtcg gcagagtcag
      841 ctttccattc gatacccgca taaatgtctt ccgagttttc acggaagata accatatcgg
      901 tcagttcagg gtgtttaacc gggcttggag tgccctgata gtaacgtacc ggacgcaggc
      961 agatgtagag atccagttcc tgacgcaggg caacgttgag agaacgaata ccgccaccaa
     1021 ccggagtggt cagcgggcct ttaatggcaa cgcgatattc acgaatcaga tcaagggttt
     1081 cagcaggcag ccagacatcc tgaccataaa cctgtgtgga tttttcaccg gtgtaaattt
     1141 ccatccagga gattttacgc tcgcctttat aggctttctc gactgcagcg tcgaccactt
     1201 tcagcatggc tggggttaca tctacaccga ttccatcacc ttcaatgtaa gggataatcg
     1261 gattttcagg aacgttgagt ttgccgtttt gcagggtgat cttcttgcct tgtgccggaa
     1321 caactacttt actttccatt cacctctcct tcgagcgcta ctggtttgct cgtctgccac
     1381 cacgttgcat atgcgtttgc gtcctgcgat acggatgctt tagagcaatt ttttgttaat
     1441 gatttgtaat tgacttgtca atactaccct attgtttgcc gtcatgaaag atgcgcgtta
     1501 ttaggctata atgcggcaat tcataatctc tgaaaatacc atgcaaaaaa cttcttttag
     1561 aaatcaccag gttaagcgat tcagctcgca acgttctacc aggcgtaaac atgaaaacca
     1621 gcccacgcgt gtgatcctct tcaataaacc ctacgatgtt cttccgcagt tcaccgatga
     1681 aaccggacgc aaaacattaa aagaattcat cccggttcag ggcgtttatg cagcaggtcg
     1741 ccttgaccgc gatagcgaag ggttgctggt gctgaccaat aacggcgcgt tgcaggcgcg
     1801 tttaacccag ccgggtaaac gcaccggaaa aatctattat gtgcaggtgg aaggtattcc
     1861 cacacaagac gcacttgaag ccttgcgcaa tggcgtaacc ttaaatgatg gccctaccct
     1921 gcccgcaggc gcggagctgg ttgacgaacc cgcgtggtta tggccgcgga atccaccaat
     1981 tcgtgaacgc aaaagtattc ccaccagctg gctgaagatc accttatatg aaggacgtaa
     2041 tcgccaggtg cgccgcatga ccgcccatgt tggtttcccc acgctgcgac tgattcgcta
     2101 tgcgatggga gattactctt tggataatct tgccaatggc gaatggcgag aagtgacaga
     2161 ttaaggatgc aaaatgttta aaccgcacgt taccgttgct tgcgtggtgc acgcagaagg
     2221 caaattttta gtcgttgaag agacgattaa tggtaaagcg ttatggaacc aacctgccgg
     2281 gcatctggaa gccgatgaaa ccttagtgga agccgccgcc cgtgaactgt gggaagaaac
     2341 cggcatcagc gcgcagccgc aacactttat tcgtatgcat cagtggattg cgccagataa
     2401 aacgccgttt ttgcgtttcc tctttgccat tgagcttgag caaatatgcc cgacccagcc
     2461 tcatgacagc gatatcgact gctgccgttg ggtcagcgcc gaagaaattt tacaggcgtc
     2521 aaatcttcgt tcgccgctgg tggcggaaag tattcgttgt tatcaaagcg ggcaacgtta
     2581 tccgctggag atgattggcg attttaactg gccttttaca aagggtgtca tctaaaaggc
     2641 tgcgtgatag aatacgccgc cttgaagttc aatgtcgtga gtgatccaat gtctgaaacc
     2701 gcaaaaaaag taatcgtcgg catgtccggc ggtgtcgatt cctccgtttc tgcctggctg
     2761 ttgcaacaac agggatatca ggtcgaaggc ctgtttatga agaactggga agaagacgac
     2821 ggtgaggaat attgcacagc agcagcggat ctggctgatg cccaggcggt ctgcgacaag
     2881 ctcggcattg aactgcacac cgttaacttt gctgccgagt actgggacaa cgtcttcgaa
     2941 ctgttccttg ccgaatataa agccggtcgc acgccgaatc cggatattct gtgcaacaag
     3001 gagatcaaat ttaaagcctt cctcgaattt gccgccgaag atttaggtgc cgattatatc
     3061 gctaccggtc attacgtacg tcgggccgat gtcgatggca agagccgcct gctgcgtggt
     3121 ctggacagca ataaagacca gagctacttc ctttatacgc tcagccatga gcagattgcg
     3181 caaagcctgt tcccggtagg cgaactggaa aaaccgcagg tgcgtaagat tgctgaagat
     3241 cttggtctgg tcaccgcgaa gaaaaaagac tctaccggca tttgcttcat tggcgaacgt
     3301 aaattccgcg agttcctggg ccgttatctc ccggcgcaac cgggcaaaat cattaccgtc
     3361 gatggcgatg aaattggtga gcaccagggg ctgatgtatc acactctcgg tcagcgtaaa
     3421 ggtctgggta tcggtggcac caaagaaggt accgaagaac cgtggtatgt ggtggacaaa
     3481 gacgtcgaaa acaacattct ggttgtcgct cagggccatg aacacccgcg gctgatgtct
     3541 gtcgggttga ttgcccagca gctgcactgg gtcgatcgcg aaccattcac cggcactatg
     3601 cgttgcacgg taaaaacccg ctatcgccag accgacattc cgtgcaccgt caaggcgctg
     3661 gacgacgatc gcattaaagt gattttcgat gagccggttg ccgccgtgac gccgggccag
     3721 tctgccgtct tctataacgg tgaagtgtgc ctcggtggcg gtattattga gcagcgtctg
     3781 ccgctgccgg tctgattatt atctttactt aaaacaagga agcagtgaac gtggcaaaga
     3841 attactatga catcaccctc gccctggccg gtatttgtca gtcggcacgc ctggtgcaac
     3901 aactcgctca ccaggggcat tgtgatgccg atgcgctaca cgtctcactc aacagtatta
     3961 ttgatatgaa ccccagctcg acgctggcgg tttttggcgg tagcgaagcc aacctgcgcg
     4021 tcgggctgga aaccctgctc ggcgtgctca atgccagcag ttgccagggc ttaaacgccg
     4081 aattaacccg ctacacactc agcttgatgg tgcttgagcg caaactctcc tcagcgaaag
     4141 gcgcgctcga cactctgggc aaccggatca acggcctgca acgccagctc gaacacttcg
     4201 atttacagtc cgaaacgctg atgagcgcga tggctgctat ctatgttgat gtgatcagcc
     4261 cgcttggccc gcgcattcag gtcaccggtt cccctgctgt actgcaaagc ccacaagtgc
     4321 aggcgaaagt tcgcgcaacc ctgctggcag gcattcgcgc cgccgtgctc tggcaccagg
     4381 tcggcggcgg acgtctacaa ctgatgtttt ctcgtaatcg cctgaccact caggcaaaac
     4441 aaattcttgc tcatttaacc ccggagttgt gatctatgga attatcctca ctgaccgccg
     4501 tttcccctgt cgatggacgc tacggcgata aagtcagcgc gctgcgcggg attttcagcg
     4561 aatatggttt gctgaaattc cgtgtacaag ttgaagtacg ttggctgcaa aaactggccg
     4621 cgcacgcagc gatcaaggaa gttcctgctt ttgctgccga cgcaatcggt taccttgatg
     4681 caattgtcgc caatttcagc gaagaagatg ccgcacgcat caaaaccatc gagcgtacca
     4741 ctaaccacga cgttaaagcg gttgagtatt tcctgaaaga aaaagtggcg gagatcccgg
     4801 aactgcacgc ggtttctgaa ttcatccact ttgcctgtac ttcggaagat atcaataacc
     4861 tctcccacgc attaatgctg aaaaccgcgc gtgatgaagt gatcctgccg tactggcgtc
     4921 aactgattga tggcattaaa gatctcgccg ttcagtaccg cgatatcccg ctgctgtctc
     4981 gtacccacgg tcagccagcc acgccgtcaa ccatcggtaa agagatggca aacgtcgcct
     5041 accgtatgga gcgccagcac cgccagctta accaggtgga gatcctcggc aaaatcaacg
     5101 gcgcggtcgg taactataac gcccacatcg ccgcttaccc ggaagttgac tggcatcagt
     5161 tcagcgaaga gttcgtcacc tcgctgggta ttcaatggaa cccgtacact acccagattg
     5221 aaccgcacga ctacattgcc gaactgtttg attgcgttgc gcgcttcaac accattctga
     5281 tcgactttga ccgtgacgtc tggggttata tcgcccttaa ccacttcaaa cagaaaacca
     5341 ttgctggtga gattggttct tccaccatgc cgcataaagt taacccgatc gacttcgaaa
     5401 actccgaagg gaatctgggc ctttccaacg cggtattgca gcatctggca agcaaactgc
     5461 cggtttcccg ctggcagcgt gacctgaccg actccaccgt gctgcgtaac ctcggcgtgg
     5521 gtatcggtta tgcgctgatt gcgtatcaat ccaccctgaa aggcgtgagc aaactggaag
     5581 tgaaccgtga ccatctgctg gatgagctgg atcacaactg ggaagtgctg gcagaaccaa
     5641 tccagacagt tatgcgtcgc tatggcatcg aaaaaccgta cgagaagctg aaagagctta
     5701 ctcgcggtaa gcgcgttgac gccgaaggca tgaagcagtt tatcgatggt ctggcgttgc
     5761 cagaagaaga gaaagcccgc ctgaaagcga tgacgccggc taactatatt ggtcgagcta
     5821 tcacgatggt tgatgagctg aaataagcct caaatcagtg ccggatggcg atgctgtccg
     5881 gcctgcttat taagattatc cactttttct tttttcactt tacctcccct ccccgctggt
     5941 ttatttaatg tttaccccca taaacacata atcgcgttac actattttaa taattaagac
     6001 agggagaaat aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta cgtcaccacc
     6061 ttaaagttca gattcaggat gctggtcatc aggtcgatga cgcagaagat gccaaagaag
     6121 ccgattatta tctcaatgaa catttaccgg atattgcgat tgtcgatctc ggattgcctg
     6181 acgaggacgg tctgtcactg attcgccgct ggcgtagcaa cgatgtttca ctgccgattc
     6241 tggtattaac cgcccgtgaa agctggcagg acaaagtcga agtattaagt gccggtgctg
     6301 atgattacgt gactaaaccg tttcatattg aagaggtgat ggcgcgaatg caggcattaa
     6361 tgcggcgtaa tagcggtctg gcttcacagg tcatttcgct ccccccgttt caggttgatc
     6421 tctctcgccg tgaattatct attaatgacg aagtgatcaa actgaccgcg ttcgaataca
     6481 ccattatgga aacgttgata cgcaataatg gcaaagtggt cagcaaagat tcgttaatgc
     6541 tacaactcta tccggatgcg gagctgcggg aaagccatac cattgatgta ctgatgggac
     6601 gtctgcgcaa aaaaattcag gcacaatatc cgcaagaagt cattaccacc gttcgcggcc
     6661 agggctacct gttcgaattg cgctgatgaa aaaattactg cgtctttttt tcccgctctc
     6721 gctgcgggta cgttttctgt tggcaacggc agcggtagta ctggtgcttt cgcttgccta
     6781 cggaatggtc gcgctgatcg gttatagcgt cagtttcgat aaaactacgt ttcggctgtt
     6841 acgtggcgag agcaatctgt tctataccct tgcgcagtgg gaaaacaata agttgcatgt
     6901 cgagttaccc gaaaatatcg acaagcaaag ccccaccatg acgctaattt atgatgagaa
     6961 cgggcagctt ttatgggcgc aacgtgacgt gccctggctg atgaaaatga tccagcctga
     7021 ctggctgaaa tcgaatggtt ttcatgaaat tgaagcggat gttaacgata ccagcctctt
     7081 gctgagtgga gatcattcga tacagcaaca gttgcaggaa gtgcgggaag atgatgacga
     7141 cgcggagatg acccactcgg tggcggtaaa cgtctacccg gcaacatcgc ggatgccaaa
     7201 attaaccatt gtggtggtgg ataccattcc ggtggagcta aaaagttcct atatggtctg
     7261 gagctggttt atctatgtgc tctcagccaa tctgctgtta gtgatcccgc tgctgtgggt
     7321 cgccgcctgg tggagtttac gccccatcga agccctggca aaagaagtcc gcgaactgga
     7381 agaacataac cgcgaattgc tcaatccagc cacaacgcga gaactgacca gtctggtacg
     7441 aaacctgaac cgattgttaa aaagtgaacg cgaacgttac gacaaatacc gtacgacgct
     7501 caccgacctg acccatagtc tgaaaacgcc actggcggtg ctgcaaagta cgctgcgttc
     7561 tctgcgtagt gaaaagatga gcgtcagtga tgctgagccg gtaatgctgg agcaaatcag
     7621 ccgcatttca cagcaaattg gctactacct gcatcgtgcc agtatgcgcg gcgggacatt
     7681 gctcagccgc gagctgcatc cggtcgcccc actgctggac aatctcacct cagcgctgaa
     7741 caaagtgtat caacgcaaag gggtcaatat ctctctcgat atttcgccag agatcagctt
     7801 tgtcggtgag cagaacgatt ttgtcgaggt gatgggcaac gtgctggata atgcctgtaa
     7861 atattgcctc gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc tctatattgt
     7921 ggtcgaggat gatggccccg gtattccatt aagcaagcga gaggtcattt tcgaccgtgg
     7981 tcaacgggtt gatactttac gccctgggca aggtgtaggg ctggcggtag cccgcgaaat
     8041 caccgagcaa tatgagggta aaatcgtcgc cggagagagc atgctgggcg gtgcgcggat
     8101 ggaggtgatt tttggtcgcc agcattctgc gccgaaagat gaataaatat gtccttttac
     8161 cactacatca agtcattgat tcgatcaaac aattttcatt gaaaaagtgc caaaaatcgc
     8221 aaattgacta caccattaac tacaccgttc ggtgcactgt atgaaacaac gtggaacaaa
     8281 tagacacaag aaatatacag gcgggtaatc tttccagggg gaagcgcacc aattcatgag
     8341 gggcgttaat gtcgatatgg ggatccccat aacggggcta ccggattttt tttccggtta
     8401 acctttaatc aggctggtgg gttttacata cccttgatca ccgtaatgat gatccagatg
     8461 tgcacctttt ccaccaatgg cggaaaagct ccaaccgtaa cgatgatcgc tatggttgcc
     8521 tcgatggtct gcgtggtcat tatgaccgtt cagccagtga ccttaatcag aatttttcgc
     8581 taaaaaatga cgttggccac gtcatccgga aagcacaaaa tcacgtcgat ttttaatgaa
     8641 cggttaaacc gtctttttgt cacggtgtgt cactggttgt cactctccgt cacgattgag
     8701 gtggtggtca ttctgcccga ccgcgcatct ttgcgctatc gggaatatca aacagttacc
     8761 tcgccgactt cctcaaattg aggtttccga aaatatcaga gcgggttagc gattaagctg
     8821 acgagtaatt aaactgcgaa aatttcgtag tttctgaata tattccggcc tgctggttaa
     8881 cagtcctcag atgtgaggga tgtagccagc gcaattttgc gctttgcttt aatctcagtg
     8941 agttaccgcc gtaaccgctc cggcttcttc cagtggtacg tgattttctc cttctcccga
     9001 tacagttcaa cgcggcgatt gtaggccagc atttccagaa cgcggatccg tatgtcgcgc
     9061 atatccgcat cattaagctg gataccatca cggcgcatca cctcagcaac aacacgcgca
     9121 taattttcgg ctgtcacgct gtccggctgc gtggtctgtt cgtcagcctg ctggctgatt
     9181 ccagagacgc ggcggattaa tcgcagtatt tcggcttctg tcatgctgct gacctcatta
     9241 caccccgcta aattcttcca gtttctggcg gtggctgtcg cttatatcaa aagcaaaatc
     9301 ctcatgctct gcctggaatg taccaaacgc catcagcgcc gctacgctcg ggtctatctt
     9361 gttcggtgat tttttcttgt tcggcttgat attggcgttc gcgtcactct gcatcacgac
     9421 gttactcatc gaccaggaca acaccggatc gccacgatgc acaatcaccc tgcggttaac
     9481 aaaaacttcg aacgatttcg ccgccggact gaatctgagg taggtttgcg ggaacggctc
     9541 cacctcaaaa ccagccccct gtaattgcgt cctgagatgc gtggcgttcc acgtatcaaa
     9601 gcccaccagc ctgatattaa atttctccgc atcctgcatg atgtcatctc tgatccggtc
     9661 gtaatcaatg cagtcacctg gcgttgtgcg tatccagccc gctttagccc actggcgata
     9721 gacagcgcgg tttttattgg cggggttctg tagctggaac tccggcagat aatgacggga
     9781 aatcagcata atggttttac cgaccggaaa ggcatagcat acgctggaaa tatcgctggt
     9841 tgatgataag tccagccccg cgtaacactc ctgaccgtgt aaatcttcct ccgtgaacgt
     9901 tccggcacac tcagcccatg caccattacc catccacggg gtagtcccct gacaccagat
     9961 attaaatcgc tttgtcatca tctccaccca ctgcgacgga ataccccgcg ctttctggat
    10021 ggttgaggcc agtttttcac gatccacgga aacatcaata ttagggttcg cctttatcca
    10081 catcgccgga tcatcaacct cgttttcgtc gtccagttcg taaatcagta caaaaattga
    10141 atcgttaacc tcttcgccgt ccaggatctg gcagcaataa tcatagtgct gtttacaggc
    10201 tgaaacgacg ttactgcccg atgtggtaat ggcaaataac agcccttcgg gacgtgcgcc
    10261 catccccagc tcaagcgcgg aataaacgcc gttatcgggg tgtaggtggt attcgtccac
    10321 gatggaaaga ctggggtttg tcccctcgat ggttgccgct tttgctgcca gcggctttaa
    10381 caggctgttg cttttcgggt gtatcacctt atgcgcctga atattcaccc gcctgcgtaa
    10441 cggtcgggat aaaaggcaca tctgacgcgc attatcaaac acgatccgcg cctggtcacg
    10501 gctcaccgct gcggtgtaga tatcctgctg accgttctcc ataatcagaa accagttagc
    10561 cagaatcgcg gcggtcgtgg atttcgcatt tttgcgcggc acttcgataa aggcgctcgt
    10621 gtatttgcgc cgtccggtgg ccttaacctt aaagccgagg atgcacgcaa aggcgaactg
    10681 ctgccacggc tccagctcaa tgggtctgcc acgcatcggt ccttttacgt gcgggcacac
    10741 cctggaaaag gcaataaacc gctccacaac ctcacgatcg aacgtgtaaa gggggctttt
    10801 aaggtccgaa aagtaccgtt taacagcctg ttttaaccgt ttacaggccg gaattttgcc
    10861 cgtttttacg tcttctgcgt acttattcca ggcggtcaag ctcgtcctct tcttctgttt
    10921 ccggtggatt tttacggcgg cttatcgggt caaaaccgag caaggaggcc atttttatca
    10981 tcactctttc agcgtcggat tttgcgctta atgcggggtt tctgctctcg ccgccctgac
    11041 tgttaacaat gctgaacccg cgcgccgcaa ggtctgcaac ggctttccgg taaatggagt
    11101 agttgacgca atacagttcc agattgctcc agtcggcggg ggtcaggtct ccccgttccg
    11161 caagctgccg cgatttttcc cgccactgct tcacggcgat atcatccagg taggcggggt
    11221 cgggtttgtc ctgaaaacgc cccccacccc gtcatacagc ctcatcagcg attgcggaaa
    11281 cattccataa cctcgcggtc acggtcggtt aatcgcttcg ctgtgatgcg ttctgcgcgt
    11341 cctgacgctt tatctttatg ccctgtttcc tgtgtcttcc atgcgtcacg ctgccttata
    11401 agtccacgga taaggcggtt ttgttcccgc tcattcatca gcgtcataca tccagttatt
    11461 gcggttagca gcccgttctt cctcttcacg aaatccacct gcggcacgct tgctttttgt
    11521 tgccggatca agccatttcg ttttctggtt atgacacgcc tggcacaatg gttgatggtt
    11581 ccattcgggc cagaagagaa catcatcacc gccattaatc gggataatgt gatccaccac
    11641 cacggcgggc gtatatatcc ccttctcaag gcatcgcacg cataacgggt ttttactcag
    11701 atacatggcg cggtatttgt cccactgtct ggaatatcca cgcgcgcggc ggtgtcctcg
    11761 tctggcatcc tctgcacgcc atgccgcccg cctgtgctcc tcacacttgc cggatttaac
    11821 gcgcctgtta cagcctggtt ctgtgcatcg tcttaatggt tgccacggca tcagtacccc
    11881 cccacatcac ggtaagccgt ccagagtgcg cctatcgtca tgggtacgcg tgtttttgcg
    11941 ttatccgcga caatctggcg attttcatac aggtgagcga taaacatcat gcagccaatc
    12001 tttatggctg gcgtgaacgc cagcccgtca tcaaagcgcc tgcctatgtg tatctggcac
    12061 gcctcaagcg atgcggcaat gtatccgctg attaactggt cttcatcgtc gccatcgatg
    12121 cggcaatgga gtttcacttc ttccagggtg ataagttctt ctgtcatttt tccgcgccct
    12181 cacgacaaag aatttcaagg cgtgtcctgg cggcatccgg cagcggctgc ccgatgatat
    12241 tcagtacacg ccccgccagc ggccccgtac tgacctttat ccggctggtg gcgttgatgt
    12301 ccttccggta ccgtatccag atccttacgg taccaatcgc cagctctgcg cccgatgaaa
    12361 tggcctcctt gctgctgacc atgttcacgc ttgcccagag tgtgtgaccg tcctcccacg
    12421 tttcgagtat ttcgccggtt atatccctgg tctgtttcag ggtctgaatc gttatcctgt
    12481 cacgcagtcg ccctatgttc attccggttt ttctccctcg ctgattttta cttcctgttt
    12541 ccatgcctgg ctgaactcat caccaccatc acgcggtgac agcccttcac gttcgcgggc
    12601 ttcgttcgga cacataacgt cggattttat gccgcgctca taggtggcaa agcgttcgcc
    12661 aggtgtggcc cgtaacaggt ccgcgctgtc aaactccacc tgataccgga taccaggtac
    12721 aggcgaggcc accagcaagg cgttttttat ctgctgctca aagttcgcca gccacgggcg
    12781 catcgtcatg gtgagaaatg cgcggcttgc ctcgctgaag ttgctgtagg tgctgttgct
    12841 gtattcctgc aaaaaaatcg gcgagacgtt gaacattcgg gcgatgtctt caatggtgaa
    12901 gcgacgggag gccagccatt cggcatcctg attactcatg cccagctgct gatagctcat
    12961 ccccccttca aggatgggcg tttttccggc gtttctggcc cctttgtagc gttccagtgc
    13021 ggctaatgcc tgtttgccct tcacgccgtc cagccattcg cctgacgtga taacccctgc
    13081 cgccatcatg ccatcacgca tcacgctcgc gccgtggcgt tgttgggcca gcccaagccc
    13141 cagcgattcg cggcagatgg ttacaggtga gcgccccata aaaccgtcat ccgtggagta
    13201 acgaaggtgg agaacctccc acggtaaata gttgcggatg tttccggtgt aggcgtcagt
    13261 gatgcagtag cgccagttat gatcacctgt ctgctccacg ttcaccgact gcggcgggta
    13321 aggatgtaaa gccgccggaa aaccatcacg cccccactga ataaccgcat aagcattacc
    13381 acttaacagg cagtagcgga tcatcattcg cttaaactgg tagggtgtct gccacgcgtt
    13441 cgggcgctcg ttgaggatgt gatcgaccgg atgagaatca agccattcgc gggcctcctt
    13501 ccccttctca ttgcgtacca ggtacaggta acacggcatg gtagccaccg cgtcagagat
    13561 gaccgtgacg gcgttcatga cggcggggag tgattccgct gtcccagatg atacgtactc
    13621 acccgccccc gtgttcgatg tgccggccag cgccataaat tcatcaagcg tcatgctgcg
    13681 ctgctctttt tttcgtctga aaggccacat atcacacccc cgctaaatcc gcccaccagc
    13741 ggcgattatc cgcacgcggc attttttcgg ggtgctgctc atacagggaa cggcgggcca
    13801 gctccacgcc ggaatcgggg taagccggta ccgatgtaac ggtaatttcg tacagttccg
    13861 ccaccagcac ggtgcgcacg catggatctg ttgtggtatc ccatacatcc ttacgggagc
    13921 gaaagccaaa gctcatcccc gagatgtccc cacgcttaac cagttcgata acgtcgcgcc
    13981 ctgtactggt atccggtggg gtcagttcaa agcgtaaccc tgtttcgtcc tcttccagtt
    14041 tcagcgtgcc ggaacgggtg cgccccagta acatgctgtg gtcatgctca tacaggccgc
    14101 gaacgtcatt acccgccgca agccattcag taaacgcccc ccgctggaat ttttcgtaaa
    14161 actcacccca taacagttct gaaagcttat cccagcgaac aacgtagccc gtcagcgtac
    14221 cggctccgct ggtggtgatt tccgatgacc ggatttccat actcttcata attttttcac
    14281 ccataaaaca ctaaaggggc tttttagccc cttctgtatg ctgttaatcg tcgtcttccg
    14341 gcagtgccag aatcttgatc gcgttcgaat ccaccacgcc accgcctaaa tatttttgcg
    14401 tgaaaatttt gatgaatcct ggttctgtca ggttgtccgg tctggtgcga acacctgttt
    14461 cgtgatcaac aatgtagtaa ccgcgtttga agtcacccag ggcaataacg ttatcaggca
    14521 taaactccag atattcgacc ggaaggccca gcaacgtatc aggatcaccc gcctgtaaac
    14581 ggtcgcgcca gatataatca ccgttcgcgt tcttcacctt ctgaagtttt gccgccgtcg
    14641 tggagttaac cactcagacc gcgtttttgc ggtatttttt acgtaatgca aatttcaggt
    14701 cgatcagcgg gtccgcagat gtccacgcca gagattcgga aggtttaatt acctgcaacg
    14761 taccaaaatc acgctctttg tcgttcttct ctgcacgggg tacggataaa aaaccttttg
    14821 cttttttgtc accgtcgccc acaaccagat cgctttcttc ggtttccgtg aaggtgtcgc
    14881 caatctcacc cgtcagccat gaaaggatgt ccacatcgga aaaatccacg atttcctgtg
    14941 tggtgcgcgg gtacgcatag accggataca gcttaatgct cacctcgtta atctgcgggg
    15001 tgctggtctg ttcgcgtgcc ttaccctctt caccgtggtt aacggtcgca ccgccagcgg
    15061 aaacaagctg cttaaactcg ttgctgctga ttttcttcac ggtacagatg cgacgcatgg
    15121 tggattcatc cgccagcatt cgcatgattt cggtgttcag ttcagggata acggtataac
    15181 caccatcagc gggaacgcct gtacttaatg cgcgggtttc accggtcaga atgtagttac
    15241 gtagttctgc ggggtcagtg gtctggctgc ttttacctgg cttgctgcgc tcttcgtctg
    15301 caatggcttc aaggcgggaa atgtctttat cgagggattc agctttagcg cgtaattcgt
    15361 caaattttgc gccctcagca tcgttaagac tgcggttttc tttttcagcg ttctccagca
    15421 tgtcgcgcat ctgattttta atggcggttt tctgctggcg taattcgatt attctctgca
    15481 taaaaaaagt cctggggtta agtaaggaac tccaggacgc ggcaaacact caaccgtttt
    15541 tcataaggaa atcagtaatc gcaccgatcg ttttcccgcc tggtaatgaa tattggcgag
    15601 cacattaaca ggcgggaaag tggccccagc gtcctggcac cacgggcgag aataatcatg
    15661 attcagttcg ggtaaaatat gccgatccgc tcagtgaaca acgtggaaca accacgaaca
    15721 aataatttac aaaaaataac aaaaagccgg attgctccgg ctgcgtggtg tggttctgat
    15781 attcctactc tgctaatttg ctcagcccca tgtctgcgta agtgcgattt actgcatttc
    15841 tcaggtctgc gtagttctcc gttggcggct ccggtgtgcc cttgctgctc tgttttacca
    15901 gcgggtcgcc caccagctgc ccgtaagctg caatttgtgc tgtcatattg ccacctcagt
    15961 gaagcgatcc ggcattgttc tttttcatct cttccatcat tttttcagtg agcattacgc
    16021 actgaaaccc catgtttttg atgtctacgg gtttcatgcc tttgtcagtg gatatcacag
    16081 gcagaatatc ctgatacaca tcgcggtcag ggtaactaag cacacaaagc tcccatgatg
    16141 ccacgggtga ggatccttcg tctgtttcaa cccaccagcc tggtgtagct gcgcttattt
    16201 gcagaatcgt gatccgtccg ttgtgcttgt aaaatttttc accaaatttt ggttcgctca
    16261 tgattccacc tctccggttt taacgttgat tgttgttacc tgttccgctt cggcaatctc
    16321 ccgctctgtc agcgtggcaa aatttgccgc cgtcgtggtc atgaatgcgc ttatcagttc
    16381 gggatgtgct ttcgcgtatc cttcccccgc gtggcggtct atcgttctga ttgccacctt
    16441 taaagcgtgc tctgtcatgt ctaacgcgcg atatttcggt tctgttctgt ctctgcgttt
    16501 tttgaatgat ttattaaatt tccctgaagt gtgcatattt atttttaccc cctcgtttaa
    16561 aaagttttga gttgtgcctc cccttgtcta ccttatctac cttagtggcc ctcatgccag
    16621 taatggcgct gctttcagcg gggtagagtg cttttatcca ctatctaccc cgtgtctacc
    16681 tccctgtctg attcaggtaa aatcaggtag agagggtaga tagtgggtag acagtaaaaa
    16741 aaggctatct acctaactta atgcactgaa ttaaatgtat ttttctttac tcaggtagac
    16801 agggtagaca gcaattacaa aaaattataa aaacgcgtcg cactcgtctg ttgttattgc
    16861 gttagtctgc gttactccct taactttccg cgtaatatat tcatgtccgt aaactttcgc
    16921 ggctggcttc atagccttgc caaagtcatt tacgtttagc ggtttgctcc tgcctgcgta
    16981 cgccataaac gccagataga cgcggtaaag gctgtttctg gtcgtgtact tcactgaatc
    17041 gccaccgccg cccatcatca ggccgcgtgc ttcctccaga aaattcagga actggcaaaa
    17101 ctcaataacc ggatccgtct gttgctttat tgccagagct tcatcaccgt cacgctgttc
    17161 cagtagtaaa gcccgtgctt tctcagagtc ggtaaagttc gccagcaatc ggcggataat
    17221 gacagggatt tcagccgcaa tcttttccgg tagctccctg tctttttcgg cctcactaac
    17281 gatattgtcg aaacggaaaa tcacgcgacg acgtgccaca cctccggccc gttcggtgaa
    17341 tatcatcggg ttattgttgg tcgccagcac caccgccctg attacagccg tgaaacgctt
    17401 ttcgtatttc gggttaattt ccacggggtc accgcccgtg attttcttga tgcccgttcc
    17461 ttcgcctgta tatttcggct ggtcagccag gacgataaga cgactcccga caacctgcgc
    17521 acgtccacca gcatcatcaa gcgatgtcat ttcagcgctt accgtgttct gtttccctgc
    17581 cagaaggctg gctatgtgtg tgaatgtact tttaccgctc ccgccgtctc cggtggcctc
    17641 aataaacatc tgccagtcgt accggttcgc cataatcatg tacagcgcgg cacatatacg
    17701 catcatcttg cgctggtctt ttccggctgc gtgctcaagc catttatgaa agtttagcgc
    17761 gttatcgcgg atgttctccc ctggtgctgg tggcgtgtac tcaatgccgt tgtgcgtggt
    17821 gatccagttc tccggcgtgt gcggggaaaa ttcccccgtt ttcaggtcaa gcgcaccatt
    17881 ggcgaacggc agcaaatcgc cggacggctc gcccattggt tcggcaataa cttttaacgc
    17941 ttccacggcg ttattgatta cgcgcttgct gaaagtggcc ctgtgctctg aatagatcac
    18001 caccatttcg cggctaagtt ccattgtgct gaccggacac cataccccgc cgcgccatac
    18061 gtgaacgatt tcactttcct gatgcacaca aacgccatca aagcgctcgg caagcagctg
    18121 cgcgcgctca ctgtctgcca tttgtgccag ttgcgtattt ttctgctccg gcatggtaag
    18181 ccctgcggca atattttcac gctcagcatt cagatagcgc cgccagtttt cacacttctg
    18241 gacgtgcatt ccttcggggt aaaaatttgc gtcctgaata tctgccgccg ccagcttctg
    18301 accaatcttt ttggtctcca ccaaatccag ttctccggcc tggtacagcc ttacgcgttt
    18361 ttttccatcc ggaacaattt tcagtgcatc aagttcggca agctgatttg gcccaagcca
    18421 cacaggcggc acattatcgc cggatgcggg gccatcctgc tcctgccatt gtttcgcgtg
    18481 cgaccaggca tcactacccg caaagataat tacctctgtg tctttgtgtt ttattccgcg
    18541 tggctgtttt tttacgttcg gtgccagttt cattttttac ccctgaatac gttaagcatc
    18601 ttttttattt cctgaatatt ggcgcgtgct ttctccctgc tggttggttt actgcggggc
    18661 gctgcctgta ccagcgtgaa atcacgccgg aattgataaa caggcatcac gcaatcatat
    18721 tcgtaacctt cacggcggta ggtgatgcgc cgttctgcca cgcctttaat cgttaccatg
    18781 ccgccgtact ggtcgcggta aatatcaccg cgcgtaaatt tagggtgagt gttgccactg
    18841 gcagttaagc cagaatattt aagtttcatt atttttattc tccggtgtgt tgttctttat
    18901 atctgtcgtg caatagatct atttcttgca gttccattat tacaggctca agaagcgtta
    18961 ttaatgccgt ggcaattctt gatttttgtt tgtcgcgttc attgtcgcca agtgtttcaa
    19021 gccatatgcg caatatttcc agcatgtttt cactgtgaga aagtgcaaga aatgcgcggt
    19081 ctattgtttc gtggtaaata tcacgcatat taatccccgt ccgtcgtttt tcttaaaata
    19141 acctctgtca cgaaatcagc ataatcggcg gcgatattca gtacatcaag cccgttgatt
    19201 tatattctct cgtggaaagt aagaaaaaag ccgctctaat aagttctggc attgacgaaa
    19261 gcgcatcagc cgcatcatca ggaacgccgg aaaattcctg tttcagggaa ttaaaacgat
    19321 catcacgcat aacccccccc attttcacaa tcggcaacaa gaatattttt agcgtcattc
    19381 agtgaccgcg ttgcgatgta tcggatacat tccagggcga actgtgtccg gtctttctgt
    19441 tcttctccct gcgcaagctc tgccgtgcat tcaatatcaa taagcgcgtg catcagcgta
    19501 gtgagtgcgg cggctgctgc gttcggtgtg gttttattgt gcataggttt ccccctgacg
    19561 aatacgggcg gcgaatacag caacacaacc ggacgggcaa cggctacgcg cttcgcgttc
    19621 cgtccaagcg gttacgtgga tgatttggga ttctccggca ctcagtgcca gaaaacgcca
    19681 cacaaaggcc gtttgtgtgt gtacaaggtg tggtatatga tttacggcaa ccataacggc
    19741 tcctagttta cgttgttggt tagaagcccc gttactgctc ctacagtgcg gggctttgct
    19801 ttttgtgttg attgtgtgta atgtgtcact acacataaat acattacctg aggtgtaatt
    19861 aacgtgtcaa cacacaaaaa cgaaaggcgt ggtaatcctc cgttccagtt ccgtttagat
    19921 cctgacttac gggaaaagat ggaaaaggca caacaacagg acggcgatga ttctcttgct
    19981 gcctggataa agcgaataat ccgcaaagaa ttacagcaac gcggcataga acccaaagga
    20041 taaaaactat cagcgccgtg gtgtgaggta ctacggcgca ttgctttaca ggcgcacaca
    20101 atgaccaacg aagaatcaac cgaaaaaaca tcacctgcac gaaaaagacg acgcagaaag
    20161 aaagagcatg aatcagaacg attcgcgccg tgttcttttg cccttgagaa gtaccttaaa
    20221 gaatactcat caaaggagag aacttcgcag ttatggcaac gcactgaacc agactgattg
    20281 cattgcccac cagcctgata gcggctatca tccccgtgct tatgtttggg atcacacaca
    20341 caaacggcgc agcgggttat ctgttcagaa aggcggctcc atttcggggc cgcttttttt
    20401 atgcctgaaa aaccccaatt ttgtggtttt ccagtttcac cagggcgaac gaatccccgc
    20461 ccacgttctg gcgtatattc aatcttcatg gttatagctc tgtgttcaga tgattgatgt
    20521 gtggcggctg cgtgccgcca gcgtgattaa tgaactgcct tgcagctatc cttccaggcc
    20581 agaacctcgg ataaagacca gccaacggaa cgaccgccaa gtttacgacg tgatgggaat
    20641 tgtccggcct tttccaggcg gtagcggcat gagcggctaa ggcctgttag cttttcgcat
    20701 tctttttcac gtataaaccg atcagtgctt aacactattg ccccctttcg tttcttaaag
    20761 agttatttcg tgttctattg cgttgggatg tgtctgattg tgtcaggatg attcagagtt
    20821 ggcaaatgtt gaggccgtat ggtttacaga aagaggaata atcaggataa aatcctttaa
    20881 attcatgtta atacaaaggc ataaaatatt gtttcatgcc ttttttctcg ctatttaaag
    20941 agtgattcgc tagtgtataa aaaatcagta acacattaaa aatcagctac ttataaatct
    21001 gtacactttt tcacctcttg ttcgtagttg ttccgcattg ttgctcattg ttgcacgttg
    21061 tatctgttcg catatccagt atgcgcatac tgaaaaaaca cgaaaaaaat tattttcttc
    21121 tggctactgg tagcgtggtt acgttttcat gtgttcccgc cagtatccct aaccgctccg
    21181 tccacatatc cagcgcattg cgtttagcat ccagataacg ggaatgatta taaactcgct
    21241 gcattcctgg catctggtgg cctgtaagct gctccacgac gtgaggatcc acgcctaaat
    21301 cgttcagcat cgttgtaaag gtgcgccgga tgtcatgtag tgaccagtga gggtgattta
    21361 gcctcctgtg cgctaatctg ccgtactgcg acacgcttgt ttcctgtttc acttccccca
    21421 gcaataagcc cgtgtgcctg ttctgctcca ccagctgcgt gacgaacggc agtattgctt
    21481 ccggtatggg ccggaatatt gccaccttcg ttttgctgtg ttctttcggc acggtccaga
    21541 gcatttcggt aaaatcccac tcgctgatct ccgataacct cagttctacc gtccggcatc
    21601 cgaacacaat caggaggcgg attaacgcga tgtagtaggg ggagaatatt tttttgtcca
    21661 gtgcctgcaa taattcgccc agttctttgg tgcttaagac acgctcgctt atatccggtt
    21721 ttttcccaac gtccgccaca ctcatatcat caagaacgtt gctgattgca tagcgccgcc
    21781 tccggcagaa cttaagcgcc tgtttgcacg tctgtagcaa gaatccggca gtaacaggcg
    21841 ttcgctttgc cacctggtca aaacaggcca gccagtgccg tagctcgcat ttatccagcg
    21901 gcatagcacc aatgtgctgt attacgtgat tattaaggcg ctttttcagg gcggcataat
    21961 ccacgcggtt ttcctttgcg tacgactcaa gccagtaggt gagcgcatcg ccaaccgtta
    22021 ccggctttaa cgcttcctgt acggtgtaat taagctcatg acgtggattt ttcccctctg
    22081 ccagccatgc gcgacactgg gcggcttttt ccctggctga tttcaggctc agatcaggat
    22141 aatttcccag cttaatgcgt tccggtggtg ccccccttcc cgttccggcc ctgtaagtga
    22201 aataccaggt taaaaggcca ctggttgaat gcctgacgct caggtttcca ccgtcattaa
    22261 gaaaggctgt tttttgggcg ggtgtgccgt tgattttcct cagctgtgta tcgctcagtt
    22321 tgttaagtgc tctgctcata attttgattc cggtcatcac aattaactac accattaact
    22381 acaccgatcg ttgcacaaag ggctacaacg tgaaacaagc tggaacaagg aaaatcacaa
    22441 atctgtttat aatcaaaaac ataatgaaca atctgaaaca ttatgaaacg gcaaaaaaca
    22501 ctaattgata aaatatgtcc atacttcacg cattacgtta agcatccgtt ataatcggtt
    22561 gcagatacca gcctgtggat gcttaacatg gaataccaac tcactcttaa ctggcccgat
    22621 tttcttgaac gtcactggca gaaacgcccg gtggtgttaa aacgcggctt taataatttt
    22681 attgacccga tctctccaga cgagttggcg ggtctggcga tggaaagcga agttgacagt
    22741 cgactggtca gtcaccagga tggcaaatgg caggtcagcc acggcccgtt cgaaagctac
    22801 gatcatctcg gtgaaaccaa ctggtcatta ctggtacagg cagtaaacca ctggcatgag
    22861 ccgaccgccg cgctgatgcg accgttccgt gaactaccgg actggcgtat tgatgatctg
    22921 atgatttctt tttctgtacc tggcggcggc gtcggcccgc atctcgatca gtacgacgtg
    22981 tttatcattc agggtaccgg acgtcgtcgc tggcgagtgg gcgaaaagct gcaaatgaaa
    23041 cagcactgcc cacatccgga tctgttacag gtcgatccgt tcgaagccat catcgatgaa
    23101 gagctggagc ctggtgatat tctttatatt ccgccaggat tcccgcatga aggctacgcg
    23161 ctggaaaatg cgatgaacta ttccgtgggt tttcgcgcgc caaatacgcg ggaattaatt
    23221 agcggatttg ccgattatgt gctgcaacgt gaactgggcg gcaactacta cagcgatccg
    23281 gatgttccac ctcgtgctca tcctgcggat gttctgccgc aagagatgga taaactgcgt
    23341 gagatgatgc tcgaattgat caaccagccg gaacacttta agcaatggtt tggcgagttt
    23401 atatcccagt cacgtcatga actggatatc gcgccgccgg aaccgcctta tcagccagat
    23461 gaaatctacg atgcgctgaa acaaggtgat gtgctggtgc gcctgggtgg tctgcgcgta
    23521 ttgcgcattg gcgacgacgt gtatgccaat ggtgagaaga tcgattcccc gcaccgtccg
    23581 gcactggatg cactcgccag caacattgcg ctgactgcgg agaattttgg cgatgcgctg
    23641 gaagatccgt cattcctcgc gatgctcgcg gcgctggtca atagcgggta ttggttcttc
    23701 gaagggtaag tttggattta agccggatgc agcatcacat gccgcatccc ttttcgctta
    23761 cttccgttgc gccgttaact cggcaatacg gacgatcacc tgcaccgctt tttccatacc
    23821 ttccagaatc acaaactcat gcttaccatg atagttgtaa ccgccagtga acaggttcgg
    23881 gcaaggtaat cccataaacg acaactgcgc gccgtcggta ccgccgcgga tcggtttcag
    23941 ttccggttca atatcgcagt cacgcatcgc ctgctgggcg atatcgagaa tatgcggatg
    24001 ctcaaccact ttctcgcgca tattgtagta actgtcttca atcaccaatt caatgtagca
    24061 atcaggatgt aaccctttgc ccactttttt ggcgatctcc atcattttac gtttacgcgc
    24121 ttcaaactgt ttacggtcga aatcacggat gatgtagtgc atatcggccc gttcaacggt
    24181 gcctttcatg ctcgccaggt gatagaaacc ttcatagcct tctgtcattt ccgggctttc
    24241 atccgccgga acttccgcat gaatacgtgc cgccagcgac agcgcattta ccatcactcc
    24301 tttcgccgtg cccggatgaa cattgttacc gacaattttt atattgaccg atgcggcgtt
    24361 gaagttttca aactccagtt cgcctacgcc accaccgtca acagtgtaag cccagcgggc
    24421 atcgaaggca tcaacatcaa aatgtttcgc ccctttgccc acttcttcat ccggggtaaa
    24481 ggcgacgcga atatcaccat gcggaatgtt tttctgttgc aataccgcca gcgcggtcat
    24541 gatttctgca atacctgctt tgtcatcagc acctaacaag gttttaccat cggtggtaat
    24601 caacgtctga cccagtagct gatgcagcac cgggaacata accggtgata aaacttcatc
    24661 gccgataccc agcgcaatat cgccaccgcg atagttttca acaatttgcg gattaacatt
    24721 tttgccgctg caatccggtg aggtatccac atgagaaata aagccaatcg ccgggatatc
    24781 gccagggacg ttagccggta acgtcgccat caaagtgccc ttctcactta aggtcacatt
    24841 gataagcccc atttcttcga gctgctcttt cagcagatgc aataacttcc attggccttc
    24901 cgtgctggga acctgtctca cccctgcttt tgattgggta tccagagaca cgtagttcaa
    24961 aaatcgctca agtagtttat ccatgtagtc accctcactt tttgtgacaa cattattaag
    25021 aagcaagaaa agacaaatat tgcgtcaggt cacttttacc cctgcaagcg ggaatattta
    25081 tcagcattac cttaatgaat ataaagctaa gccagtaatt cacaacaaag attggcgatt
    25141 caagcggttt gccgtagaat taccgccctc attaggagtc accaaggtgg ttaaccacaa
    25201 accccgcatc ggtaagccat ccgttgcgtt tacatgggac agagtaaaaa attgaataaa
    25261 caaccgagtt cgctttcacc gctggtgcaa ttggcgggaa ttcgcaaatg ctttgatggt
    25321 aaagaggtca ttccccagct ggatctgact atcaacaatg gcgagttcct cacgctgctt
    25381 ggcccttctg gctgcggtaa aacaaccgtt cttcgcctga ttgcaggtct ggaaactgtt
    25441 gattccggac gcatcatgct ggataacgag gacatcaccc acgttccggc ggaaaaccgc
    25501 tatgtgaaca ctgttttcca aagctatgca cttttccccc acatgaccgt gttcgaaaat
    25561 gtggcctttg ggttgcgcat gcaaaaaacc cccgctgctg aaattacgcc ccgcgtgatg
    25621 gaagccctgc ggatggtgca gctggaaacc ttcgctcaac gtaaaccgca tcagctctct
    25681 ggtggtcaac agcaacgcgt cgccattgct cgagcggtgg ttaacaagcc tcgtctgttg
    25741 ttgctggatg agtcgctctc agcgctggat tacaaactgc gtaagcaaat gcagaacgag
    25801 ctgaaagcgt tacagcgtaa gcttggcatt acattcgttt tcgtgactca cgaccaggaa
    25861 gaagcactca ccatgtcgga caggattgtg gtgatgcgcg atggtcgcat tgagcaagac
    25921 ggcacgccgc gtgaaatcta cgaagagccg aaaaacctgt ttgttgccgg gttcattggc
    25981 gaaattaata tgtttaacgc cacggtgatc gagcgactcg acgagcagcg cgtacgcgcc
    26041 aacgttgaag gccgcgaatg taatatctac gttaacttcg ccgttgaacc ggggcaaaaa
    26101 ctgcatgttc tgctgcgccc ggaagactta cgtgttgaag agattaacga cgacaaccac
    26161 gctgaagggc tgattggtta cgttcgtgag cgtaactaca aaggcatgac gctggagtcg
    26221 gttgttgaac tggaaaatgg caagatggtg atggtcagcg aattcttcaa tgaagacgat
    26281 cctgactttg accactctct cgaccaaaaa atggccatta attgggtaga aagctgggag
    26341 gtcgtactgg ctgatgaaga acacaagtaa gttccagaat gtagtgattg tcactattgt
    26401 cggttggctt gtgttgtttg tctttctgcc caacctgatg atcattggca ccagcttttt
    26461 gacccgcgac gacgccagtt tcgtcaaaat ggtctttaca ctggataact acacgcgtct
    26521 gctcgatccg ctctattttg aagtgctatt gcactcgctg aatatggcgc tgatcgccac
    26581 cctcgcctgc ctggtgctgg gctatccatt tgcctggttt ctggcgaagc tgccacacaa
    26641 ggtgcgtccg ctgctgctgt ttctgctgat tgttccgttc tggaccaact cattgattcg
    26701 tatctacggg ctgaaaattt tcctcagcac caaaggctat ctcaacgagt ttttgctctg
    26761 gctgggcgtt atcgacacac caatccgtat catgttcacg cccagtgcgg tgattatcgg
    26821 tctggtttac attctgctgc cgtttatggt gatgccgctg tactccagta tcgaaaaact
    26881 ggataagccg ttgcttgagg cggcgcgcga tctcggtgcc agcaagttac agacttttat
    26941 ccgcatcatt attccactga cgatgccggg gattattgcc ggatgtctgc tggtgatgct
    27001 gccagcgatg ggcctgttct atgtgtccga cctgatgggc ggtgcgaaaa acctgctgat
    27061 cggtaacgtc atcaaggtac agttcctcaa tattcgtgac tggccgtttg gtgcagccac
    27121 cagcattacg ctgactatcg taatgggcct gatgttgctg gtttactggc gcgcttctcg
    27181 tttgctgaat aagaaggtgg aactcgaatg atcggtcgac tgcttcgcgg cggttttatg
    27241 accgctatct acgcgtacct gtatatccca atcattattt tgattgtgaa ctcctttaac
    27301 agctcgcgct ttggcatcaa ctggcagggt tttaccacca aatggtatag cctgctgatg
    27361 aacaacgaca gcctgttaca ggcagcgcag cattcactaa caatggcggt gttttcggcg
    27421 acgtttgcta cgcttatcgg ttcactgacg gcagttgcgc tgtaccgtta tcgctttcgc
    27481 ggtaagccgt tcgttagcgg aatgctgttt gtggtaatga tgtcaccaga tatcgtgatg
    27541 gcaatttcgc tgctggtgct gtttatgctg ctgggtattc agcttggctt ctggtcgctg
    27601 ctgttctcgc atatcacctt ctgcctgcca tttgtggtgg tgacggtgta ttcgcgcctg
    27661 aaaggttttg acgtgcggat gctggaagcg gcgaaagatc tcggtgccag cgaatttacc
    27721 attctgcgaa aaatcattct gccactggca atgccagcgg tggcggcggg ctgggtgtta
    27781 agctttaccc tgtcgatgga cgatgtggta gtttcttcat tcgtcaccgg accaagttat
    27841 gaaattctgc cgttaaaaat ttattcgatg gtcaaagtcg gcgtatcgcc ggaagttaac
    27901 gcgctggcaa ccatattact ggtgctgtcg ctggtgatgg ttattgccag ccagcttatt
    27961 gctcgtgata aaacgaaagg taacacaggg gacgttaaat gaaaaaatgg tcacgccacc
    28021 tgctcgcggc gggtgctctg gcactgggca tgagcgccgc tcacgccgat gacaacaaca
    28081 cgctgtattt ctacaactgg accgagtacg tgccgccagg actgcttgaa cagttcacca
    28141 aagaaaccgg tattaaggtt atctattcga cttacgagtc gaacgaaacc atgtacgcca
    28201 agctgaaaac gtacaaagac ggtgcctatg acctggtggt tccttcaacc tattacgtcg
    28261 ataaaatgcg taaagagggg atgatccaga agatcgacaa gtcgaagtta acgaacttca
    28321 gcaatctcga tccagacatg ctcaacaagc cttttgaccc gaataacgac tactccattc
    28381 cgtatatctg gggtgcgacg gcgattggtg ttaacggtga tgcggtggat ccgaaatctg
    28441 tcaccagctg ggccgatctg tggaagccag agtacaaagg cagcctgctg ttgaccgacg
    28501 atgcccgtga agtgttccag atggcgctgc gtaagctggg ctactccggt aacaccaccg
    28561 atccgaaaga gattgaagct gcatataacg agctgaaaaa actgatgcca aacgtcgcag
    28621 cgtttaactc cgataacccg gctaacccgt acatggaagg cgaagttaac ctcggcatga
    28681 tctggaacgg ttctgctttc gttgcacgcc aggcgggtac gcctattgac gtggtgtggc
    28741 cgaaagaagg cggcatattc tggatggaca gcctggcgat cccggcaaat gccaaaaaca
    28801 aagaaggcgc gctgaaattg atcaacttcc tgctgcgccc ggatgtggca aaacaggttg
    28861 ctgaaactat cggttatcca acgccaaacc ttgcggcgcg taagctgtta agtccagaag
    28921 tggcaaacga taagacgctc tacccggatg ctgaaaccat taaaaatggc gaatggcaga
    28981 atgacgttgg cgcagccagc agcatttatg aagagtatta tcagaagctg aaagcaggac
    29041 gttaattcag caagcgggcg gtggtattac cgcccgtttt ttagttagca aggatgtggc
    29101 taatgagtca ggacagtaaa gttttatccc gctctttcgt cggtattgtg gtccgcttgt
    29161 aggcctgata agcgtagcgc atcaggcaat gcttcctgct ttataatccc tccagcaact
    29221 tttcaacaaa ttctggcacc acctggcttg ccgggccgta atatttctcg gcaaattcat
    29281 taccaacctg actcggttca agattcagtt caacggtgtg agcaccatgt agtttggctt
    29341 cgtgtacaaa cccagccgcc ggatagacat gcccggaagt accaatggca ataaagacat
    29401 ccgccatcga caacgccata taaatttcat ccatgccgag tggcatttcg ccaaaccaaa
    29461 cgacgtgcgg gcgcaagggt gccgggaact ggcaacaatg gcatttatct tctggcgtaa
    29521 cgtctccggt ccagtcgaga acctgaccac tttgcgaaca acgaactttc agcaattccc
    29581 catgcatatg aatgacattg gtattacctg cgcgttcatg caggttgtct atattctgcg
    29641 ttaccagcaa aaagcgatcg ccgagagcgt cttgcagttt agccagcgca agatgcgcgg
    29701 cgttaggctg aatttctggc tgctgcagct gtcgacgacg ggcgttataa aacgcttgca
    29761 ccagttcagg atcgcgatcg aaaccttccg gcgttgccac atcttcaacc cgatgttctt
    29821 cccacaggcc atctgcggcg cgaaaggtac gaatacctga ttccgcagaa attcctgccc
    29881 ctgtcagtac gagtactctt ggtttttcca ttgcttccgg caccacttta tctctgaaaa
    29941 aaatacgctg acgcaaacgc tcgcgcaggc gacgtttatt tttacgaaaa cgacttaacc
    30001 gatgaccccg acgcgacagc atagcaacct ctgttgttta atcggttaga tgtaggaagg
    30061 ccgcaccacg cattcctccc gcatcaccgt ggcgcgcgcg ttcaatgcgt ggaacacgag
    30121 ctacaggtaa gagatgacaa ggcaacctgt ccgccagttg cgttgtgatt gccgggaaat
    30181 tcgataagcc accaccaatg acgaccaggt cagggtcaac aatggtcagg atatttccca
    30241 gagaaaccgc taataaatcc agataacgct caacgtgcgc ccttgcctgc tcatcgcctt
    30301 gatcataaag cgcaataatt tcgggagcct gcaacggttg atgataatag tgttgataca
    30361 gccacgcaaa accgcgacca gacagataat tttcaatgca gccatgctga ccacagccgc
    30421 agcggcgtaa cgggaaatcc aaccccatca tggttaacgc atcaaccggc agacgcatat
    30481 ggccaaactc gccggtaatg tagcttttac cagtaatcgg tttgccgttg aaaaccagcc
    30541 cgccgccaac gccggtgccg agaattaacc ccatcaccag tggatattgc gtaaattcat
    30601 catcccaggc ttctgaaagg gcaaaacagt tggcatcgtt atcaaggcgt acatcgcgat
    30661 caagacgtgc gctcaggtcg gcacgcagcg gtttgccgct ggcagcgggg acattagcgg
    30721 catacagctt accatcttct gtttccggca ttcccggaat accgatgccg acagaacctt
    30781 tacagccaaa acgttggtca gcttcagcta ccagctcaca cactgcatct aaaaatgcgt
    30841 catagctgtc acgcggtgtc ggcacccgct tttcccactg caactgccga ccgctatcaa
    30901 acacgccaag cgcaattttt gttccaccaa tatcaaaccc gtaatacatt gccgctcctt
    30961 gttttaatgt actgccttta ctggccgcta aggactcgcg cagggtcaat attactggcg
    31021 cgccgcgccg gataccaact tgccaaaaga ctcagcaaca atgctgtgac cagtacgtag
    31081 aagacgtcca gccagtgcaa ttccgatggc aggaagtcaa taaaataaat atcgctggag
    31141 aggaactgat gaccgatcag cttttcaatc cactcaataa tcggggtaag ttgcagtgaa
    31201 acaactacgc cgataaccac cccacacagg ctgccgaaca gccctgccag caatccatac
    31261 cagacaaaga tggcgcgaat taaaccatct ttcgccccca gcgttcttaa tactgcgata
    31321 tcgccactct tgtctttcac cgccatcact aaggtggaga cgatgttgaa acaggccacg
    31381 ccaatcacca gtaccatcgc cagatacata atggcgcgga tcatctggat atcgcgatac
    31441 atatagccgt aagtaccaat ccaactttta atataaacat agctgttggt cacttcaccc
    31501 gcatcgcgta ccagcttatt ggcgttgaaa acatccgtca ttttaagggc aatacctgac
    31561 acgctggaac ccatatcaag atattgttgg gcatccgcca gcgggatcat ggcaaaactg
    31621 tgatcgagtt gaccactcaa ctgcaaaata ccggcaatgt gcaaacgcac acgttttggc
    31681 tgcatcagtt tatgctcggg attcgagttg gggatcatga tcgacaccca atcgccctgc
    31741 ttcactttca gcgcatccgc cacgcctttg ccgatgataa tttgctgttc gcccgcttta
    31801 aaattgcgcc acgcatcccc ctgaacaaac gagggtaatg cgctcagacg ctgttcctgt
    31861 tgtgggttaa cgcccttcac ctggattgcg cgcagattcg ctccactttc caccagaccg
    31921 gtgaaattga tatacggcgc agcggcggca atacccggca ctttctgcac gttatccagt
    31981 gcttcctgcc agttagtcca tggctgattt accgcttcga tttcgccatg cggcaccacc
    32041 gccagaatgc ggttattcag ttcgcgttca aagccgttca tcgcgcttaa gccgacgatc
    32101 aataccgcca cgccgagggc aatgccaatg gtagaaatga cagagattag cgacaccatg
    32161 ccgccgcgtc gccgtccgcg actaaaacgc aggccaatca ataacgataa aggcatcgcc
    32221 attactccgc ccccatcagg ctcagttccg ccgtcagacg tccatcacgc atttccagtt
    32281 ggcggctcat acgtttcgcc agttgcaggt cgtgagtgac caccaggaaa gcggtgccct
    32341 gcaagcgatt caactcccca agcaactgga agatgctgtc ggcgttacgc gcatcgaggt
    32401 taccggtagg ttcatccgcc agtaccagac gcggattatt gaccagcgca cgggcaatcg
    32461 ctacacgctg gcgttcgccg ccagaaagtt cagatgggcg gtgattcgca cgatgctcca
    32521 gccccaccgc ttttaacatc tccagtgcac agctgttgat ttcagcgggc tttttcttgc
    32581 caatcagcag tggcatagcc acgttttcca gggcagtaaa atccggcagc aggtggtgaa
    32641 actgataaat aaagcccagc ttctggttgc gcagttctgc tttcgccgct gaagacagtt
    32701 tgctcatcgg ctgaccgtta aagatcacat cgccggaggt tggcgtatcc agcccgccta
    32761 acaggtgcag caaggtactt ttaccggaac cagagctacc gacaattgcc atcatttcac
    32821 cttcgcccac gctgaaactg acattgagca gcacatcggt ttgcacactg ccttcctgat
    32881 agcgtttgca caggttgtcg cattgcaaca ggatcttatt cataacgtaa agcctcagcg
    32941 ggttgtgtgg cggcagcgcg ccatgaaggg taaagcgtag acagcagcgc gatagccatc
    33001 gccaccagcg caataacaat gacctgtaaa ggttcgatag ccaccggcag cgccgcgcca
    33061 tcaagcagga cgccgattat cggcatcaga ttatttaact ggctggcaag cagtgcgcca
    33121 agcgccgctc cgaggattgc accgataatc ccggcgctag ccccttgcac cataaagacc
    33181 atcatgattt gtcgcggagt taagccttgc gtttgcagga tcgcgacttc gccctgcttc
    33241 tccatcacca tcagccccag cgaggtaata atgttaaacg ccgcaacagc gacaatcagg
    33301 ctcagcagta agcccatcat atttttttcc atgcgtacgg cctggaacag ctcgccttta
    33361 cggtcacgcc agtcctgcca tttgctgcct tcaggcagtt tttgctgact taaagagtca
    33421 actctcagcg gctcatccag ccacaaacgc cagccggtaa tattggctgc cggataacgc
    33481 atcagacgcg aggcatcctc aatattcacc agcatttcat agccatcgac ttcactgttg
    33541 gcggcgaaag tgccaatcac attgaacagg cgctgacttg gaatacgccc catcggcgtg
    33601 aactggctgg cagatggcac catcacgcgg atttgatcac cgcgattgac gcccaactgt
    33661 gaggcaagtt gttcgccgag gatgacatta tatttccccg gctcgaggtc agtttgtttc
    33721 acattgacca gatacggcgt tagtggatct ttttgcgccg ggtcgatacc gagcatcacc
    33781 ccgaccgcca cgctgcgcgc gctttgcagt accacatcac cggtagtaat aggtgcgacg
    33841 cgattaacgc cgtccagttt gactgccgtt tccgggagtt gctgcgggtt aagagagcca
    33901 tgctcagaag agagaattgc ctgtggcatc aggccaagga tgttgttttg cagctcgcgc
    33961 tcaaagccgt tcatcactga caatactgtg accagcgcca tcaccccgag ggtaatgccg
    34021 atggtagaaa gccaggagac gaaacgaccg aagcgatcca ctgcacgccc acgcatgtaa
    34081 cgcaggccaa taaatagagc gacaggttgg tacatgaaat ccgtctggtt gctgtagcaa
    34141 agacacgagt atataagcga aagcaagatg ggtaaacgtc tgaactgtaa gtgatcgtca
    34201 gggctaatct gaaaagcccc cgtaaatcaa tcggctaaat tgccatattt tatcatcaat
    34261 cttgccgcct gatcttaatc tgaaaaatca tccaatacag acagataccc attacacctc
    34321 gggtcataac cagcaggatc aaccgcatgt aaattaaaaa agagcgtgtt gttctgatga
    34381 aacaaaaaga gctatggatt aaccagatca aagggttatg tatttgtctg gtggtgattt
    34441 atcactcggt cattaccttt tatccgcata tgaccacttt tcagcatccg ttatcggaag
    34501 tcctgagcaa atgctggatc tatttcaatc tttaccttgc cccctttcgt atgccggttt
    34561 ttttctttat atccggctat ttgattcgcc gctatatcga cagcgtaccg tggggaaatt
    34621 gtctcgataa acggatctgg aacatcttct gggtgctggc gctttggggc gtggtgcagt
    34681 ggctggcgtt aagtgcgctg aaccagtggc tggcacctga gcgcgattta agcaatgcct
    34741 ccaatgccgc ttatgccgat tccaccggtg agttcctgca cgggatgatc accgccagca
    34801 ccagcttgtg gtatctgtat gcgttaattg tctatttcgt ggtatgtaaa atttttagcc
    34861 gtctggcgct gccactattc gccttgtttg tactgctgag tgtggcggtt aatttcgttc
    34921 ccacgccgtg gtggggaatg aacagtgtga tacgcaattt gccttattac agccttggcg
    34981 catggtttgg cgcaacaata atgacctgtg ttaaagaggt gccgttgcgc cgccatctgc
    35041 tgatggcttc tttgcttacc gttctggcgg tcggtgcctg gttgtttact atctcgctgc
    35101 tgttgtcgct ggtatcgatt gtggtgatca tgaagctgtt ttatcagtac gagcaacgat
    35161 tcggtatgcg ctccaccagc ctgctgaatg tgattggctc caacaccatt gctatctaca
    35221 ccacccatcg cattctggtt gaaatattca gcttaactct gcttgcgcaa atgaacgcag
    35281 cacgctggtc gccgcaagtc gaactgacac tcctgctggt ttatcccttt gtcagtttgt
    35341 tcatctgtac tgttgcgggc ttgctggtaa gaaaactttc acagcgtgca ttcagcgatc
    35401 tgttgttctc cccgccttca ctgcccgctg ccgtcagtta ctctcgttaa accatcaccc
    35461 cagccggggc gattcaattt gctaaaccat gtcgattacg gataatgaac agcattgctt
    35521 atcagagata tgcccccata tgttgaggca tatcctaacg agaatctgac aaccgttatg
    35581 cctgaacaat atcgttatac gctgcccgtc aaagcgggtg agcagcgtct gctgggcgag
    35641 ttaaccggcg cagcctgtgc aacgctggta gcggaaattg ccgaacgtca cgccggtccg
    35701 gtggtactca ttgcaccaga tatgcaaaat gctctgcgtt tgcatgatga aatcagccag
    35761 ttcaccgatc agatggtgat gaatctggcg gactgggaaa ctcttcccta cgacagtttt
    35821 tcgcctcatc aggacattat ctcctcgcgc ctttccaccc tttaccagct accgacgatg
    35881 cagcgtggca tactgattgt tccggtgaat acgcttatgc agcgcgtttg cccacacagt
    35941 tttctccacg gtcatgcgct ggtgatgaaa aaaggtcagc gcctgtcacg agatgcatta
    36001 cgaacccaac tggacagcgc cggttatcgc catgttgacc aggtgatgga gcacggcgaa
    36061 tacgccacgc gcggcgcgtt gctggatctc ttcccgatgg ggagtgagct gccttatcgt
    36121 cttgatttct ttgatgatga aatcgacagc ctgcgggtgt ttgacgtcga cagccagcgc
    36181 acgctggagg aagtagaagc gatcaatctg ctgcccgcgc acgaatttcc gaccgataaa
    36241 gcggcaattg aactgttccg cagccagtgg cgcgatacct tcgaagtgaa gcgcgatccg
    36301 gaacatattt accagcaagt gagtaaaggc acattacctg ctgggatcga gtactggcag
    36361 ccattgttct tcagcgaacc actgccaccg ctgttcagtt atttccctgc caataccttg
    36421 ctcgtgaata ctggcgatct ggaaaccagt gccgaacgtt tccaggctga cacgctggcg
    36481 cgttttgaga atcgcggcgt cgatccgatg cgcccgctgt tgccaccaca atcgctctgg
    36541 ctgcgggtgg acgagctctt ctcagagctg aaaaactggc cccgagtgca gctaaaaact
    36601 gaacatttac cgacaaaagc cgcgaatgcc aatttaggtt tccagaaact gccagacctg
    36661 gccgttcagg cgcaacaaaa agcgccgctg gatgcgctgc gtaagttcct cgagactttc
    36721 gacggtccgg tggtgttctc ggtagaaagt gaaggtcgcc gtgaagcgct gggtgaactg
    36781 ctcgcacgaa ttaaaattgc tccgcaacgc attatgcgtc ttgatgaagc cagcgaccgt
    36841 gggcgttatc tgatgattgg cgctgccgaa catggttttg tcgataaggc gcgtaatctg
    36901 gcgctgatct gcgaaagcga tctgctcggt gaacgtgttg cccgccgtcg tcaggattct
    36961 cgccgcacca tcaaccccga tacactgatc cgtaaccttg cggagctgca tattggtcag
    37021 ccggtggtcc atctggagca cggcgtcggt cgctatgccg gaatgaccac gctggaagct
    37081 ggcggcatta ctggcgagta cctgatgctc acctatgcca acgacgccaa actgtatgtt
    37141 ccggtgtcgt cactgcatct gattagccgt tacgcaggtg gcgcggaaga aaacgccccg
    37201 ctgcataaac ttggcggcga tgcgtggtca cgcgcgcggc agaaagcggc ggaaaaagtg
    37261 cgtgatgtgg cggcggaatt gctggatatc tacgcgcaac gcgccgccaa agagggcttc
    37321 gcgtttaaac acgatcgtga gcagtatcag ttattctgcg acagcttccc gtttgaaacc
    37381 acgccggatc aggcgcaggc cattaatgcg gtacttagcg acatgtgtca gccgctggca
    37441 atggatcgtc tggtgtgcgg cgatgttggc tttggtaaaa cagaagtggc gatgcgcgcc
    37501 gctttcctgg cagtagataa ccacaagcag gtagcggtgt tggtgcctac cacccttctt
    37561 gcgcagcagc attacgacaa cttccgcgac cgtttcgcca actggccggt acgtatcgaa
    37621 atgatctccc gtttccgcag cgccaaagag cagacgcaaa tccttgcgga agtggcggaa
    37681 gggaaaatcg atattctgat cggtacgcac aaactgctgc aaagtgacgt caagtttaaa
    37741 gatttaggcc tgctgattgt cgatgaagaa caccgcttcg gggtgcgtca taaagagcgc
    37801 attaaagcga tgcgcgcgaa cgtggatatt ctgacgctta ctgcaacgcc gatcccacgt
    37861 acgctgaata tggcaatgag cggaatgcgt gacctgtcga ttatcgccac gccgcccgcc
    37921 cgtcgtctgg cagttaaaac ctttgtccgt gagtatgaca gcctggtggt ccgggaggcg
    37981 atcctgcgtg aaattttgcg cggaggacag gtttattatc tctacaatga tgtggaaaac
    38041 attcagaaag ccgccgaacg gctggcagaa ctggtgcctg aagcacggat tgccatcggt
    38101 cacgggcaga tgcgcgagcg cgaactggaa cgggtgatga atgatttcca tcatcaacgt
    38161 ttcaacgtgc tggtttgtac caccattatc gaaaccggga tcgacatccc gacagccaac
    38221 accattatca ttgaacgtgc ggatcacttc ggtctggcgc agctgcacca gttacgcggt
    38281 cgcgtcggac gttcacatca tcaggcatat gcatggctgc tgacgccgca tccaaaagcg
    38341 atgactaccg atgcacaaaa acgtcttgaa gcgattgcct cgctggaaga tctcggggca
    38401 ggttttgcgc tggcaacgca cgatctggag attcgcggcg cgggtgaact gcttggcgaa
    38461 gaacaaagcg gctcaatgga aaccatcggt ttctcgctgt atatggagtt gctggaaaac
    38521 gccgtcgatg cactgaaagc cggacgcgag ccgtcgctgg aagatctcac cagccagcaa
    38581 acagaagtcg agctgcggat gccgtcgcta ttgccagatg atttcatccc tgacgtgaac
    38641 acgcgtctgt cgttctacaa acgtattgcc agcgccaaaa cggaaaacga actggaagag
    38701 atcaaagtcg agcttatcga tcgcttcggc ctgctgccag atccggcgcg taccctgctg
    38761 gatattgccc gtctgcgcca gcaagcgcag aaactgggga tcaggaagct ggaaggtaat
    38821 gagaaaggcg gggtgatcga atttgccgag aagaatcacg ttaatccggc ctggttgatt
    38881 ggtttgctgc aaaaacagcc gcagcattac cgccttgatg gtccgacgcg cctgaaattt
    38941 attcaggatt tgagtgagcg gaaaacgcgt atcgaatggg tacgccagtt tatgcgtgaa
    39001 ctggaagaga acgcgatcgc ttaatgtaaa tcgggggcgt ttctgcgccc ccatctgcaa
    39061 catttacaaa ttctttgcac tttcctgcac tatccgacac tgtcaccatc cataattcag
    39121 gcttatctgt ttattacaat aaccttatat ttattatgta tttttggtga tgatcaaaac
    39181 gcgtttttct cgctggctaa cgttttttac gttcgccgct gccgtggcgc tggcgctacc
    39241 ggcaaaagcc aacacctggc cgctgccgcc agcgagcagt cgtctggttg gcgaaaacaa
    39301 atttcatgtg gtggaaaatg acggtggttc tctggaagcc atcgccaaaa aatataacgt
    39361 cggctttctc gctctgttac aagctaaccc cggcgttgat ccttacgtac cgcgcgcggg
    39421 cagcgtgtta acgatcccgt tgcaaaccct acttccagac gcgccgcgcg aaggcattgt
    39481 gatcaacatt gcggagctgc gtctctatta ctacccgccg ggtaaaaatt cggtaaccgt
    39541 gtatccaata ggtattggtc agttaggtgg tgacacgctg acaccgacaa tggtgaccac
    39601 cgtttcagac aaacgtgcaa acccaacctg gacgccaacg gcaaacatcc gcgcccgtta
    39661 taaagcacag ggaattgagt tgcctgcggt agtgccggct ggaccggata acccaatggg
    39721 ccatcatgcg attcgtctgg cggcctatgg cggcgtttat ttgcttcacg gtacgaacgc
    39781 cgatttcggc attggcatgc gggtaagttc tggctgtatt cgtctgcggg atgacgatat
    39841 caaaacactc tttagccagg tcaccccagg caccaaagtg aatatcatca acactccgat
    39901 aaaagtctct gccgaaccaa acggtgcgcg tctggttgaa gtacatcagc cgctgtcaga
    39961 gaagattgat gacgatccgc agctgctgcc aattacgctg aatagcgcaa tgcaatcatt
    40021 taaagatgca gcacaaactg acgctgaagt gatgcaacat gtgatggatg tccgttccgg
    40081 gatgccggtg gatgtccgcc gtcatcaagt gagcccacaa actctgtaaa tcctggataa
    40141 aaaaaatccc cgcgacacgg catgtcgcgg ggaaaaaatc agcagtagca ggcattaatg
    40201 agggttaatg cttatttata aattactgct gttccatgga gggtattcgg accggttaca
    40261 gaagtaatac ggaaagattt tgcgcccatc tcatccgctt tttgcgccag ctgctcttcc
    40321 agcgatccca gatttgtccc cgcgttagca ctgatagtac cgactttttg ttggccttct
    40381 ggcgttgact gaacttcgac agccgcaaag ttggcaaatg acatggagct taaaatcgcc
    40441 gcagcgatga ggtttttacg tttttcataa tagtggcctt atgcagatga atgacgtcgc
    40501 aagaaattac ttaacgatcg ataactaaat gatagatgtg atctggatca catacaagat
    40561 attttttata acaatcatta attaatttaa aaatctttaa atttcagtca aatagaaagc
    40621 aattattttt atgggatctg gactggtgaa cggaggtgat tatttttata ataacctttc
    40681 attcacagtc accaggtaca acggcatcat ggcaactgac tcaacacaat gtgtaaaaaa
    40741 aagccgtggc cgcccaaaag tgttcgacag ggatgccgcg cttgataagg ccatgaaatt
    40801 gttctggcaa cacggttatg aagcgacttc tcttgcggac ctcgtcgaag caaccggagc
    40861 caaagcgccc acgctatacg cggaatttac caacaaagag gggttatttc gtgccgttct
    40921 cgaccgctat atcgatcgtt ttgccgctaa gcatgaagca cagctgtttt gtgaagagaa
    40981 aagcgtggag tctgcgctgg ctgactattt tgctgccatc gccaactgct ttaccagcaa
    41041 agacaccccg gcaggctgct tcatgatcaa caactgcacc accctctccc cagattcagg
    41101 agatatagcc aatacgttga aatcacgcca tgcgatgcaa gagcgcactt tgcagcagtt
    41161 tttatgtcaa cgacaagcgc gcggggaaat cccgacccac tgtgacgtga cacatctggc
    41221 agaattcctt aattgtatta ttcaggggat gtcgatcagc gcacgcgaag gtgcatcgct
    41281 ggaaaaactg atgcagattg ccggaacgac tttgcgttta tggcccgaac tggtgaaata
    41341 agtcgcaaaa aaggcccctc agcctgagcg aatgaggggc caaattgcag agtacagcct
    41401 tgttatactt tgttattcaa aatcagttgc ccgttactat ctagcgggat ctgcgtaccc
    41461 ggatcgcggt ccatgcggat tttgccctgc tgatcgccaa tcttataggt cacatcataa
    41521 ccgagcattt tttctgactt gtcatacacc gttttacaac gctgttgcgt agtcgtgtaa
    41581 gtatcgcttt cctggagaga gccctggatc tggttaccgg catatccacc acccagcgcc
    41641 cccacaacag tggcgacatc tttaccgcga ccaccaccaa actgatgccc tatcacgcca
    41701 ccagcaacag cgccgagcac cgacccggta atgcgatttt catcctgcac cggtcgacga
    41761 tgggtcactg tgacgttgcg acactcctga cgcggtgttt taaccgtttc cttgattggg
    41821 gttgcagaaa caacctgagc gtattgcggg ccccgttcaa acacgttcag actggccact
    41881 gccgctacgc ccaacgcagc tgcgacacca atcccgatac ccgccaacat tgatttattc
    41941 acgggaacac ctccttcttt gcagttatgc caatcatgca agcgaaggag aaaagcagct
    42001 atcagacaga agatgaaaag cgcgggaatg actggaaata cttaaggatt cggaggagtc
    42061 tgaaggaatg cctgatgcga cgcttaacgc gtcttatcag gcctaccgct cgtaccattt
    42121 gtccagggcg gataaggcgt ttataccaca tccgccagtg tacgtcgatt aatgcaactt
    42181 caaacgcgga cggataacgc ggttaatact ccccaccagc atcattaatc cggttttaaa
    42241 gtaaccatgc agcgcaatct gatgcattcg gtatagcgag atatatacaa agcgcgcaat
    42301 tcgtccttca atcatcattg agccgcgcgt caggttaccc atcaggctac caacggtgga
    42361 gaagttcgac agcgatacca gcgaaccatg atctttatac tgataatttt tcagcggctt
    42421 accgttcatc tgcgccagaa tgttgttcat tgcgcaagtc gccatctggt gtgcagcctg
    42481 agcacgcggc ggaacaaagc ccccttccgg acgcgggcat gacgcgcagt cgccaatagc
    42541 gtaaatgtct ggatcgcgag tggtttgcag cgtcggttcc accaccagct ggttgatacg
    42601 gttagtttca agaccaccga tatctttcag gaagtctggc gctttgatcc cggctgccca
    42661 taccatcaga tcagcctcaa tatattcgcc atctttagtg tgcaggccgc cttcatcagc
    42721 actggtgacc atggtttgcg tcagcacgcg aacgccaagt ttcgttagct cgttgtgggc
    42781 cgcagcagag atacgtggcg gtaatgcagg caaaatacgt tctcccgctt ctaccagcgt
    42841 tacgttcagg gcttcgttgg tcaggccttt gtaaccgtag ctgtgcagtt gcttgatcgc
    42901 gttgtgcaat tcagcggaga gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt
    42961 cactttgcca tttgcgccca ggttggcaga gtatttcagg aacagattca gcatctcctg
    43021 gtggaagcga cgcgcctggt gcgggttatc gaggaaaatg cagttctctt tgacgcctgg
    43081 cgtattgaaa tcgttagagg tgctacccag cgccatgacc agggtgtcat aggcgatttt
    43141 acgttccgga accaacagtt cacctttctc gtcgcgtagt tctgcaatag tgattgtttt
    43201 cgcttcacga tcaatatcaa tgacggaacc caactggaac tggaaaccat gattgcgcgc
    43261 atgggccaga tagctcaacg catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg
    43321 cagcagtggt ttccacaggt ggctgtggtt acgatcgacc agcgtaattt tggctttttt
    43381 cttacgtccc agcttatgcc ccagctgtgt tgccatttcc agcccaccag caccgccgcc
    43441 gacaatcaca atctttttca atggcgtagt caacgtgccc cccttaaatt tattaaccaa
    43501 ttgttaatta atggttatta acatagctct taattaacaa caggttacag caatgaaaat
    43561 gttcttcgct attgagaata acatgaatgg tgcattggtc ataccaaaat tgatgttaat
    43621 caagttttgt tgcttaaaag ataggcaact ttaccggggg agatcaaaaa atagagttta
    43681 ttgtttatta tttgagcaaa ttcggaaggg taatataggg tgaagcgtat atcgagggcg
    43741 tttggtcgtc ctgataagat gcgcagcatc gcatcaggca ttgatgctcg actgtcggat
    43801 gcggcgtgga cgccttatcc gacttacaga attcggagcg catcaggtac cggcaaggtt
    43861 acctgatgcg acgagggcat ttacccgagg gttttgaacg ctttaatgcg ctgtaaatgc
    43921 ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc agacaatctc gtaataatga
    43981 tgcaactctt cagatgtccg ctggctgtta agtgcttcat cattacgcga caaaataacc
    44041 agacaacgat cgcgattctt ctcgcggaag ttggtcacac acttagtggc aatatcggca
    44101 tactcttccg ggcgatcaat cttgccttcc atgttctcat aagggaacaa attagggttg
    44161 aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt ccgcccagta tccgcccagg
    44221 ccaacgccgc aaattagcgg acgctcgtca acgttcagtt gcaacatttt gtccacttct
    44281 ttaagcagat gctgcatatc atgtttcgga tgccgcgtac tgtagcttat caagcgtaca
    44341 tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt tacccggact gttagagtca
    44401 aaaccgtgta aatagataat catcgcttcc tcacataagc cagggttagt gacctacttt
    44461 ctcttcctgc cagcgttcat gtaactgatt cagacgggtg ctgatcgctt tccagcgagc
    44521 cgagtccatc agttcctgtc gcgaaaatga acctttatga tacaaacgtg taacacgttc
    44581 tgccttgatc ggtgacagat tatctaacac gctgacggcc cctttacgat tattgcagac
    44641 caggatcata tcgcaacccg catccagtga tgcctgcccg cgttcggcat aactgcccat
    44701 aatcgcggca ccttccatcg ataaatcgtc agagaaaatc acgccgtcaa accccagttc
    44761 ctgacgcaaa acggttttca gccagtaggg agaaccgctc gccggacgcg gatcaacatc
    44821 actgtagatc acatgcgcag gcataatggc gtcgagttta ttttcgcgga ttaaggaact
    44881 gaagaccgac atatctttag cgcgaatctc cgcttgtgga cgtggatcgc acggtgtttc
    44941 tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag tgtttcccgg tcgttttcat
    45001 tccggcttca tgcataccat caataaaccg gctggcaatt gccagggctt tttgtggatc
    45061 ggcatgataa gaacgctcgc caatcgccgc gctgatatgc ccgacatcca gcactggcgc
    45121 aaagctgata tcaatatcca tagcgatcat ttcgctggcc atcagccaac ccgcctcttg
    45181 cgccagtttg ccgccctctt ccattcccaa cagcgcagca aaggattgtg ctgccggtaa
    45241 gcgggtaaaa ccttcgcgaa aacgctgcac gcgtccacct tcctgatcta ccgccaccac
    45301 cagatgattg cgcgatgctg cgcggatctg gcgcaccagt tcacgtaact gggcaggatc
    45361 atgatagtta cgcgtaaaga gaatcagccc tcccaccagc ggatgcgcca gtatttcacg
    45421 ctcttccgcg tccagttcgt aaccttcgac atccaacatt actggaccca cactgctctc
    45481 cttattgttt tattaatagc tgccgccagg tgtcatcggc cagcctgata aattgttgat
    45541 cgccggtttg tcgccagcgg tactcaaacc accctccttt gagcatcagc agccagggaa
    45601 accatcgcct gacctgacgc cataattgcg ccggataaat cttcgcgcga gtggcatagt
    45661 cattgaccaa ttgccggtgc tgttcagtat tttccaccca caccgccgcc agttccagcg
    45721 cgatatcacc atctccagca tactcccagt cgatgagttt taaccctgat gcgctatgca
    45781 ctaaatttcc ggcgtggaca tccatatgca atggacttaa gcgtaaaggt cgtggttccc
    45841 gcgctttgcg cagacgtttt aacattcgca gccaacccac agtccgccgc gccggatcgc
    45901 tttgctgcca gtacagttcc agtaagggca acagcgttat tcgccagcca aaacgtggtt
    45961 gttgatgtag ataatacagc aagcctgcca gttcgttggt atccggcaaa tacgttttta
    46021 ccgcaccggg cagatagtcg actaccatcc agtcacggag atataaatgc ggcttcggtg
    46081 caatgcttgc gggtagttgt gataaagccc gatactggcg caagaacgcg gactgcggcg
    46141 catcaggatc gtgcggctga cgcacaacaa aacgctggcc ctgatgttca atgagaaaac
    46201 taccgccact aagcccacta ttaaacgtcg tgacgggatg aaactgcggg aaaaagcgcg
    46261 acagcaattc gtcgcgcgtg atgggattat tgctgcgaaa cggcaccttt acctgaccag
    46321 ataatttcgc ccgtctgcac cagcatcagc tgcatttgta gggtcggagc gttaacgttg
    46381 ccagaggcgc aggagtacag cacgtaatga gcgccgacat tgcgggcaat gcctatggct
    46441 ttactacggg tgcctaaact gtcctgcggc gacaaaccta actgttgctt agccatcgac
    46501 agttgctggg cggaaaccag ggtaaatttc ccgttattag ccagcgcatt tcgcagcgtt
    46561 tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt tgttaacgct atcaaccagc
    46621 aggacgctac ccgcagtcac cccgtcagcc ccaagcatct tactgaccat cggctgcatt
    46681 gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg gtgccgttcg atcttcgtgc
    46741 tcaattgggc ctggctgctg cgggatcgtc ggcaccgagg gcactgtggg gacaggctgt
    46801 tgtggctcgg ctggttgttc cggcgctggt ttcacttctt ctaccggtgc aggttcacgt
    46861 tgccccacac acccggcgag aaacatcgcc agcgcggtaa tcaaggcgta gcgactcatt
    46921 tttgtcatca agattcaccc ctttacaaat atagataaag tctgactttg tgcgccccca
    46981 gaaaattggc gctgccgtac agcgttaccg ccgaatgtgc gggaatggta acgctgcgtg
    47041 gcctttccag aggatgcatc tccagccctc tggcgtcata ccagtaaaaa cgataatgaa
    47101 cggtaacggg ttcttgcctt tcgttataaa tcgttgagga tgctgaaggt tgaatatcag
    47161 acgtcgaaag gacgggcttt tctgcactga tgcccgcagc caataaagat gactccatca
    47221 ccagcgattg ctcatcattc accggaattt ccggatgtga acgacagccc accagcagta
    47281 acaacaccag agacaccagc ccaaagcatc cttttctcat cgttacagac ctttatgcgc
    47341 cagcattggt cccagcggac ggccacccaa caagtgcata tggatgtggt aaacctcttg
    47401 tccgccatgg cggttggtat tcatgatcag acgatagcca tcttcggcaa taccttcttg
    47461 ctcagcaatt tttgccgcta cggtgatcat gcgtcccagc gcctgctcat gctcagctga
    47521 cacgtcgttc acagtcggaa tgagaatatt cggaatgatc agaatatgcg ttggcgcttg
    47581 cggcgaaata tcgcgaaacg ccgttaccag atcatcctgg tagacgatat cggaggggat
    47641 ctcacgacga ataattttgc tgaatatagt ttcttctgcc acgacatttt cctttttcaa
    47701 atagccatgc gttgcgccat gctacgccgc gccacactgc gagtatagag tatggtcgag
    47761 ttaaacctgc tctttcaatc ttaactcacg atttcttaag caaaaaatcg ttttgctgcc
    47821 gtatatatcg ccattattcc catttctgcc tgcgaatgcg ctgaaaagac attctctgtt
    47881 tggtttacac gacaaatatg aatgcgtata tttctcattt gcatttgcag caaaattatt
    47941 cgcacactaa atataaccgc tcatcaccct cctccggcat gagcctgacg acaacataaa
    48001 ccaagagatt tcaaatgctt tcaacacaat ttaacaggga taatcaatat caagtcatca
    48061 ccaaaccgtc actacttgcc ggttgcatag cactggcact attaccttct gccgcttttg
    48121 ctgcaccagc cactgaagaa acggtgattg ttgagggttc agccacagct ccagatgatg
    48181 gcgaaaatga ttacagcgta acgtctacct ctgcgggtac caaaatgcag atgactcaac
    48241 atgatattcc tcagtcggtc actattgtta gccagcagcg gatggaagat cagcagttac
    48301 aaacgctggg cgaagtgatg gaaaacacgc tggggatcag caaaagtcag gcggattccg
    48361 atcgtgctct ttattattcc cgcggattcc agatcgataa ctatatggtt gatggtatcc
    48421 ccacctattt tgaatcgcgc tggaatctgg gcgacgcact ttctgatatg gcactgtttg
    48481 aacgcgtaga agtagtgcgt ggcgcgacag gactcatgac cgggacgggt aatccatctg
    48541 cggcaattaa tatggttcga aaacacgcga cctgtcgtga atttaaaggc gatgtctcgg
    48601 cggaatacgg tagctggaac aaagaacggt atgtggcgga tttacaaagc ccactcaccg
    48661 aagacggtaa aatccgcgcg cgaattgtcg gcggctacca gaataacgac tcatggctgg
    48721 accgctacaa tagtgaaaag accttcttct cgggcattgt cgatgctgat ttaggcgatc
    48781 ttacgacgct ttcagccggt tacgaatatc agcgcattga tgttaatagc ccaacctggg
    48841 gcggtttacc gcgctggaat actgatggca gcagcaacag ttacgatcgc gcacgcagta
    48901 ccgcacctga ctgggcgtac aacgataaag agatcaacaa ggtctttatg accctgaagc
    48961 agcggtttgc tgatacctgg caagcgacac tgaatgccac ccactctgaa gtcgaatttg
    49021 acagcaaaat gatgtatgtc gatgcctatg taaacaaagc ggatggtatg ctggttgggc
    49081 catacagtaa ttatggacct ggctttgatc atgtcggcgg caccggttgg aacagtggca
    49141 aacgtaaagt tgatgcgctg gatttgttcg ctgacggtag ttatgaattg tttggtcgtc
    49201 agcacaatct aatgtttggt ggcagttaca gcaaacaaaa caatcgttca gttcatgggc
    49261 caacatcttc ccggatgaaa ttggcagttt ctacaacttt aatggcaatt tcccacaagc
    49321 cgactggtca tcacagagcc tggcgcagga cgataccaca catatgaaat cgttatatgc
    49381 tgccactcgt gtcacccttg ccgatccgct gcatctgatc ctcggcgcac gttataccaa
    49441 ctggcgggtt gatacgctga cttacagcat ggagaaaaac cacaccacgc cttacgctgg
    49501 tctggtgttt gacatcaatg acaactggtc gacctacgcc agctatacct ctattttcca
    49561 gccgcaaaat gatcgtgaca gttcaggcaa atatctggct ccaatcaccg gtaacaacta
    49621 cgagctgggt ctgaaatcgg actggatgaa tagccgtctg accaccacgt tagccatctt
    49681 ccgtattgag caggataatg tcgctcagtc caccggtaca cctatccccg gcagcaacgg
    49741 cgaaaccgcc tataaagcgg tggatgggac agtcagtaaa ggggtggaat ttgaactcaa
    49801 cggcgcaatt accgacaact ggcagctgac atttggcgca acgcgctatc ttgcagagga
    49861 taacgaagga aacgccgtta atcctaatct gccacgcacc acggttaaaa tgttcaccag
    49921 ctatcggttg cctgtcatgc cagagttgac agtcggcggt ggtgttaact ggcaaaatcg
    49981 cgtgtatacc gacaccgtga caccgtatgg caccttccgc gccgagcaag gtagctacgc
    50041 gctggtggat ctcttcaccc gctaccaggt gacgaaaaac ttctcgttac aggggaacgt
    50101 caataacctg ttcgacaaaa cctacgatac caacatggaa ggttctatcg tctacggcgc
    50161 accgcgtaat ttcagcatta ccggcacgta tcaattctga ataacacctg taaaaaaggc
    50221 agccatctgg ctgccttagt ctccccaacg tcttacggat tagtggttac ggatgtactc
    50281 atccatctcg gttttcaggt tatcggattt agtaccgaaa atagcctgaa caccagaacc
    50341 agcaaccact acgcccgctg cgcccagttt cttcaggcca gcctgatcca ctttagacac
    50401 atcagcaacg ctgacgcgca gacgggtaat acatgcgtcg aggttagtaa tgttttcttt
    50461 accaccaaat gcagcaacca gagccggtgc catttcgctg gtacctgttg cttttgcatc
    50521 ttcagtcgcg tcttcacgac ccggcgtttt cagatccagt gctttaatca gcacgcggaa
    50581 gatggtgtag taaacaatcg cataaccgat accgacgatc ggtaacagcc acagtttgct
    50641 gctgttacca gacagaacga tgaagtcgat cagaccgtgc gagaacgacg taccgtcacg
    50701 catccccaga agaatacaga ttgggaatgc caggcctgcc agaatcgcgt ggatgatgta
    50761 caggatcggc gcaacgaaca tgaagaagaa ctcgatcggc tcggtgatac cggtcaggaa
    50821 cgaggtcagc gccgcggaga tcataatacc gcccactttc gcgcggtttt ctggtttagc
    50881 agagtgccag atagcaattg cggcagctgg cagaccgtac attttgaaca ggaagccacc
    50941 agacagttta cccgcagtcg ggtcacccgc catataacgc ggaatgtcgc cgtggaaaac
    51001 ctgacctgct gcgttggtgt attcaccaat ctgcatctgg aaaggtacgt tccagatgtg
    51061 gtgcagacca aacggtacca ggcaacgttc gatgaaaccg taaatgccaa acgcaactac
    51121 cgggttctgg taagcagccc actgagagaa ggtctggatt gcagaaccaa tcggcggcca
    51181 aatgaaggac agcacaacgc cagtaaagat ggcagccagg ccagaaatga tcggcacaaa
    51241 gcgtttaccg gcaaagaagc caagatactc aggcagctta atacggtaga aacggttaaa
    51301 catgtacgct gcaatcgcac cggagataat ccctccgagt acgccagtat ccgccaagtg
    51361 tttagaggcg atttcttcag caggtaaatg cagtaccagt ggcgcaacca cggccatggt
    51421 tttaaccatg atgccatagg caacaactgc ggccagcgcg gatacgccat cgttattggt
    51481 aaagccgagg gcgacaccga tcgcaaaaat cagtggcatg tttgcaaaga cggaaccgcc
    51541 tgcttctgcc ataacatgcg atacaacggc gggcagccag ctgaaattcg cggaaccgac
    51601 gcccagcaga atacctgcga taggtagtac ggataccggc agcatcagcg atttaccgac
    51661 cttttgcagg ttagcaaatg cattcttaaa cataattgag agtgctcctg agtatgggtg
    51721 ctttttttac gttctcacgc gtggcaaggg gggagagcct cgccgtgtac agggcatcta
    51781 agcgcccttt atttattaca cagagtaaaa taattcagtg ccaatttgtt tgacggctat
    51841 cacgtttcaa cttcagatca ttggagaagt tgcacatttt gacaaaacat atgtcacaat
    51901 atttcaaacg cggttattca ccgcccaatt ttcggcggtg aactttactc gttttagcca
    51961 ttaattacga gctttaaaaa aactcattca acggatggat tgaaggcggg aagcgtcgat
    52021 gtgaaacaga cgggcgaagt tatcggtggt tacctgcgcc agttcttcaa cggcaacacc
    52081 tttcaacaca gccatgtatt ctgcaacgtc acgaaccatc gcaggttgat tctcttttcc
    52141 tcgatgcggt accggcgcaa ggtaaggtga gtcagtttcc accagtaatc gatccagggg
    52201 gacataacgc gcagcatcgc gcagttgctc cgcattacgg aaggtcacaa tgccggaaaa
    52261 ggagatgtaa aatccgagat ccagtaattt acccgccgtt tctctgtcct ctgtaaaaca
    52321 gtgtagtacg ccaccgcaat ccgtcacttt ttcttcgcgc agaattgcca gcgtatcggc
    52381 acgggcgtca cgggtatgga cgataaccgg cttgttcagt tcacgaccaa tctggatatg
    52441 atggatgaag gactcttgct gacgtacttt agtttccggt gtgtaataat aatccagccc
    52501 ggtttcaccc agcgctacaa caccctcttc tgccgccaga cggcgtaaat cttctacatc
    52561 gtagggatca ttctggttga gtggatgcac gccacaagaa aatacgacgt tgtcacgttc
    52621 gcctaccaga tcccgcatat gtaaataacc cggtaatgtt gtggcgactg ccagacaaaa
    52681 tttcacatcg cgtgcggcgg ctttcgccag aacgtcatcc acgtccttat gcaaagattc
    52741 ataatccaga ccatcgagat ggcagtgtga gtcgactaaa aacatgatgt ctctcttaaa
    52801 gatgaggaac cggtaacaca acgcccggtt gcaggtaatg ctcaatacgc agtaaaagat
    52861 cggtgatgag aagctcgcgg ttgatgcctg tgacagacat taactgttca cgaatgtggc
    52921 aaacatcccc cagtatagcc tgcaggcacg agggagaaag atggtttgcc agttcggcga
    52981 ccaggcccgg cacatcaaca ttggtcacct gcgcagcgcc atgatggcgt tttagcgcat
    53041 ccatcagcaa cgttgccagc cagtgtaaac gcgccggagc ttgttcatga ttaagggccg
    53101 ccagcagcga ataccagtcg cccgatggca cgctatatgc caacgcctga cacaatgttt
    53161 cacgagcctg ccagttatct ccctgaaaca acgccagtgc cgcgccaggc gaaccggcgc
    53221 ttaagcgcaa tgcggcaagt aatgcatcct gtgacattgt cacttcgcgt gaaagccaag
    53281 tcacggcgta ctgttctgga ggcggcgcaa ggtaatgtaa ccgacaacga ctacgtaatg
    53341 ttgccagtaa acgttcaggc tcgcgggtag ccaggaaaaa ccaggtttct gctggtggct
    53401 cttcaagcgt tttcagcaat gcgttagccg cggcgtcggt cagtaaggca gcatcggtta
    53461 cccagacaac tttcgcacca cctaggcgtg cgtgctcatt cagcttttcg gtgacctcac
    53521 gtaccgcatc aatgcccagc gtatttttcc ctttttcggg agccagggtg tagtaatcgg
    53581 gatgcgtgcc agcctgcatc aactgacatc cacgacagtg accgcaactt ttgtggccct
    53641 gcggttgttg gcagagtaaa taacggctca gggcgtagat taaagcatca tcgcccatgc
    53701 ccggtaacgc ctgaatgagt agcgcatggt gacctcttcc ggcctgatag ctggctacca
    53761 gtttttcgaa atcaggtcgt aaccatggat accatctcat gcgtccaact ccttcaccca
    53821 gtgggtcacg gtagtgcgga ttgcatccat cacggcctcc agcggctggg tggcatcaat
    53881 ggtatgaata cttttatctt gtgcagccag ttccagatag cgggcgcggg tgcgattaaa
    53941 gaaatcgaaa gactcttgct caatacgatc cagctcgccg cgcgcacgcg cgcgttttaa
    54001 gccaacttcc ggggtaacat cgagatagag cgttaagtct gggcgaaaat ccccgagaac
    54061 agcatcacgc agtgttgcca gcatatgttg gtcaatacca cgtcctccgc cctgatacgc
    54121 ctgagtggaa agatcgtggc gatcgccaat cacccaggtg ccgttagcca gcgcaggttt
    54181 gatgaccgtt tctaccagtt gaacgcgcgc ggcataaaac atcagaactt cggctttatc
    54241 ggtaatgact tcatcgccta ccgatttgat atccagcacc aggcttctta acttttcggc
    54301 aagttgcgta ccgccaggtt cccgagtgaa aaccatgtcg cggataccca gttgctcgag
    54361 cgtctcaacc accacattac gcgcggtagt tttgcctgcg ccttccagcc cctcaatgac
    54421 gatatactta ctgcgcattt ttttccttaa gcactttcag ataatcctgc acagacttgt
    54481 tatgactggc aagattggta ttaaacgtgt gaccaccttt accatcggcc acaaaataga
    54541 gatacggcgt ttttgccgga tgcgcagcag ccttcagcga atccgccccc ggcgtagcta
    54601 tcgcacccgg cggcaaaccg gtaatggtat aggtgttata cgctgtcggc gtttccaggt
    54661 ctgcacgaga aagtttgcca ttataacgct ctcccatccc gtaaatcacg gttgggtcgg
    54721 tctgcaggcg cataccaatg cgtaaacggt tgataaatac cgaggcaacc tgatcgcgtt
    54781 cactggcaac ggcggtttct ttttcgataa ttgatgccat cgtcaccagc tgatttttat
    54841 ctttataagg cagaccgtcc gcacgccctt cccaggcgct atcgaccgct ttcaccattt
    54901 tcttgtgaac tcgcttgagt aacgcgacat cggtggtatt ggcggtatac atcaaggtgt
    54961 ctggccagaa ccaaccttca atccactccg ggttttccag ttcaagtgcc tgcgctacgg
    55021 tggcgtactt atcgtcactc agcgtgtgct tgatatacgg agcctcacgc aattgcttga
    55081 ggtagtcgct cagacgcatc ccttctacca gtcgcagcgg gaactgtgct tctttaccgc
    55141 tttccaacaa tttcagcatc tcgcgcacgg tcatctgcgg tgtaaagcgg taagtcccgg
    55201 ctttaaagtg agaaagatcc ggttcgatac gcagcagcca ttgaaaaacc cgtgggcgat
    55261 tgatgatctt atcagcataa agctgttcac cgagcgccag acgtccggtc cctggcttca
    55321 gggtaaatat cgtctcttct ttgataagca atttgctgtc ggcaagatgg cgaaccttcc
    55381 agacgcccac accagcggcg atacccagta ccaccagcaa taacaagata atcaataaca
    55441 cttttttcat gactaattcg ggcgctcaca aagtggggct aaatattcat ataacgttgc
    55501 tgacgaaaag gtgacatcgc cacaggcacg tacgggcatc actggcatta acgcattaca
    55561 aataaccatc tcatctgcct gcaacgcctc ctccagcgag gcttgcactt cgacaagctg
    55621 ataagaggat tgtgccagta aacggataca gaattgtcgc ataatgccgt taacacctgc
    55681 ctgatccagt cgcggcgtat aaactacatt gcccttccgc cagaacaaat tagccgcaca
    55741 gcattccgta acccaccctt cgctgtcaag gaccagcgcc tcatcagcgt ttgtctgctc
    55801 aagatgagag cgaatcaata cttgctcaag ccgattaaga tgtttaatac ctgcaagatg
    55861 aggattgcgc cccagccgca ccgggcttag cgccaacgtc atcccttcgt tacgcaaacg
    55921 gtcgtaatgt gcaggataag ccgtaacgga gagaatccgc gttgctggtc cgctgttcaa
    55981 tgtgctgtac cctcgcccgc cactaccgcg actgatcacg actttcagca caccattttg
    56041 ctgttctgct gccagcgttt tcatctcttg ttcaagctga ggccagaagt cacaggaaat
    56101 catcaaccgc tgacaagcat cctgtagtcg caggatatgc gccgataaca aactgacttt
    56161 accgtcgata actctggcgg tggtaaaaca accatcacca aactgcgttg cccgatcgct
    56221 tactgccagc gattcctgct tataaccgtt aattaagaac ataccggctc cttatcagag
    56281 aattgttagt gtggcagcat gttcactacg gaacaagtcg gaataaaagc taagaaaaaa
    56341 ggcccgcaag cggacctttt atatgggtgg aaaatgacaa cttagatctt tttaaagatc
    56401 aaagaaccat tagtgccacc gaagccgaag gagttacaca gcgtgtattc cattccgctg
    56461 acctgacgtg cttcatgcgg tacgaaatcc agatcgcaac cttcatccgg gttatccagg
    56521 ttgatggttg gcggaacagc ctgatcgcgc agcgccagga tggagtagat agattctact
    56581 gcacccgccg cacctaacag gtgaccggtc atagatttcg tggagcttac caacacacgg
    56641 cttgcagctt caccgaagat ggttttcacc gcctgcgctt cagctttatc gccagccggc
    56701 gtagaagtac cgtgcgcgtt aacgtagcca atctgactcg cttcaatgcc tgcatcacgc
    56761 agagcatttg ccattgccag agctgcgcct gcgccatttt ctggcggtga cgtcatatga
    56821 taagcatcgc tgctcatacc aaagccgacg agttcagcgt aaattttcgc accgcgtttt
    56881 ttcgcgtgtt cgtactcttc aagtaccagc ataccggcac catcgcccag tacgaagcca
    56941 tcacgctctt tatcccacgg gcggctcgcc gcttgcgggt tatcattgcg ggtagataat
    57001 gcacgtgccg cgccaaaacc accaacgccc agcggcgtac tggctttctc tgcgccacct
    57061 gcaaccatca cgtcagcatc gccatacgcg ataatacgcg cagcatggcc aatgttgtgc
    57121 acgccggaag tacaggcggt cgcgatagag atgcttgggc cacgcaggcc atacatgata
    57181 gtcagatgac ctgccaccat gttcacaatc gttgacggaa cgaagaatgg gctgatctta
    57241 cgtggaccac cgttcatcag agatgtgtgg ttttcttcga tcagtccgag gccgccaatc
    57301 ccggagccaa ttgcggcacc aatgcgggtt gcgttctctt ccgttatttc aaggccagaa
    57361 tcctgcatgg cctgaacgcc agcgacaatt ccatattgaa tgaaggcatc catcttgcgc
    57421 tgttctttgc gcgagataat gtcctcacag ttaaaatcct ttactaagcc agcaaatttc
    57481 gttgcatagg cgctagtatc gaaatggtcg attaggctga tgccactctg accggcaagc
    57541 agagctttcc aggtagactc tacggtattg ccgacaggag acaacatgcc cagtccggtc
    57601 acaactacac gacgcttaga cacgtttgtc ctccaaggag ggaaaaatga ttctagtggg
    57661 acaaaaagat aaaactcagg cggtcgaacg accgcctgga gatgttcact tacgcctggt
    57721 ggccgttgat gtaatcaatg gcagcctgaa cggtggtgat tttctcagct tcttcgtccg
    57781 gaatctcagt atcaaactct tcttccagag ccattaccag ctcaacggtg tcaagagaat
    57841 ccgcgcccag gtcttcaacg aaagaagcat tgttggtaac ttcttcctgc ttaacgccca
    57901 gctgttcgcc gataattttc ttaacgcgtt cttcgatagt gctcatactc ttaaatttcc
    57961 tatcaaaact cgctttcgcg atggttttcg tagtgtataa aatgttgaaa aatttgcaac
    58021 taaatcccgg caggtcttac cacgatttta cgttattttg aggccttacc ccccaataac
    58081 gcaaatcatt ttgcactaat tattactcat aaccacgcag actgcgcgca acatgagcat
    58141 tttgtgcaaa tcgcggtcag accatgtaca tcccgccgtt cacatgcaaa gtttcacccg
    58201 tgatgtaagc tgcttcgtcg gatgccagga atgcaaccgc gttggcgatt tcctgtgcgc
    58261 cgccgaggcg acccgcagga acctgcgcca ggatacccgc acgctggtca tcgctcagcg
    58321 cacgtgtcat gtccgtttca ataaagcccg gagcaacaac gtttacagta ataccgcgtg
    58381 acgcaacttc gcgcgccagt gatttactga agccgatcaa gcccgctttc gccgcagcgt
    58441 agttggcctg accgccattt cccatggtac caaccacaga accgatagtg ataatacgac
    58501 catgacgctt tttcatcata gcgcgcatta ccgcttttga cagacggaaa acagatgaaa
    58561 ggttagtttc gataatatcg ttccactctt catctttcat tcgcattaac aggttatcac
    58621 gagtgatacc ggcattattg accaggatat ccacttcacc aaattctgcg cgaatttttt
    58681 ccagaacaga ttcgatagat gccgggtcgg tcacattcaa catcagacct ttgccgttgg
    58741 cacctaaata atcactgatc gcctgagcgc cattttcact ggtcgcagtg ccaataactt
    58801 tcgcgccacg ggctgcgagc gtttcagcaa ttgcgcggcc aattccgcgg cttgcaccgg
    58861 ttaccagtgc gatttttcct tcaaaattca tgattttcct cttttaaagc tcgagcgccg
    58921 ctgccatcgc tgaaggttcg ttcagcgccg aggcggtcag ggtgtcgaca atgcgtttcg
    58981 tcaggccagt aagcaccttg cccgggccga cttcatagag atgttctacg ccttgcgctg
    59041 ccatgtactc aacagacttc gtccactgaa ccgggttata caactgacgt accagtgcgt
    59101 cacggatggc atcaccattg gtttcgcatt tcacatcaac gttattcaca acagaaactg
    59161 ttggtgcgtt aaaggtgatt ttcgctaatt ctactgccag tttgtcggct gctggtttca
    59221 tcagcgcaca gtgagacggt acgctcactg gtaacggcag cgcgcgtttt gcgccagcag
    59281 ctttacaggc agcgccagca cgctcaaccg cttctttatg accggcaata accacctgtc
    59341 ccggagagtt aaagtttacc ggagaaacga cctgaccttc tgcagcttct tcacacgctt
    59401 tcgcaatagg cgcatcatcc agaccgatga ttgccgccat agcgcccgtg ccttccggta
    59461 cggcttcttg catgaactta ccgcgcatct caaccagacg caccgcatca gcgaaatcaa
    59521 tcacgccagc gcaaaccagc gcggagtatt cccccaggct gtgaccggcc atcattgccg
    59581 gtgctttacc gccctgctgc tgccatacgc gatacagcgc aacagatgca gtcaacagcg
    59641 ctggctgggt ttgccaggtt ttattcagtt cttcagctga cccctgctgg gtcagcgccc
    59701 acaggtcgta gcccagcgcc gcagaagctt cagcaaacgt ttcttcgaca attggatagc
    59761 tcgccgccat atcagccagc attccaacgg tttgagaacc ctgtccaggg aacacaaatg
    59821 caaattgcgt catgttttaa tccttatcct agaaacgaac cagcgcagag ccccaagtga
    59881 atccaccgcc aaaggcttca agcagaacca actgccccgg cttaatgcgc ccgtcgcgta
    59941 cagcttcatc cagcgcgcac gggacagagg ccgcagaggt attaccgtgg cgatccagcg
    60001 tcaccacgac attgtccatc gacataccga gttttttcgc cgttgcactg ataatacgca
    60061 ggttagcctg atgcggaacc agccagtcca gttgagaacg gtcaagatta ttcgccgcca
    60121 gcgtctcatc aacgatgtgc gccagttccg ttaccgcaac cttgaagact tcgttgcccg
    60181 ccatcgtcag atgaattgaa ttctctggat tcacgcggtc ggcgtttggc agcgtcagca
    60241 attcaccata actaccgtcg gcatgcagat gggtggaaat gattcccggc tcttcagagg
    60301 cagccagcac cgcagcgccc gcgccatcgc caaaaataat aatagtccca cgatctgttg
    60361 gatcgcaggt gcgcgccagt acatcggaac cgacgaccag agcatacttc accgccccag
    60421 atttcacgta ttgatcggct acgcttaatg cataggtgaa acctgcacag gctgctgcaa
    60481 cgtcaaatgc cgggcaacct ttaatgtcca acatgctttg aatctgacaa gctgcgctcg
    60541 ggaaagcgtg cgtagcagaa gtcgttgcca caacgatcag gccaatctgg tctttctcaa
    60601 tgcccgccat ctcaattgcg cgtgtcgccg cttcaaagcc catggttgaa acggtttcgt
    60661 ttggcgcggc aatgtggcgt tcgcggatac cggtacgagt gacaatccac tcgtcagagg
    60721 tgtccaccat tttttccaaa tcggcgtttg tccgcacttg ttcgggcaga tagctgccag
    60781 taccaataat cttcgtatac atgtacgctc agtcactttt cggttatata ccgtcacttg
    60841 caaactgcga gttcgctggc agcgtcctgc taccgcagag ttccgctttt gccaccgtcc
    60901 agcagctcaa aaccagctgg gtatacagat tccaggcgag cggcaattcg ctgaggaact
    60961 tgtcgctgca ccgcctgcac tgcctgttca atcgcgaccg caaaagctcg ctgattggct
    61021 gcaccatgac tttttatcac cgtgccgcgc aatcctaaca gacaggcgcc gttatactgg
    61081 tcggggttga ggtgactgaa tcgcctcgtc aggctctttt gtagccaacg ctttaataac
    61141 agtagccacc acgaccgttt tttcccttca ccctgagatt tcagcagaga aaggaacatc
    61201 ctgacaacac cttccatcgt ctttaacgtg acatttcctg taaagccgtc acaaaccagc
    61261 acatctgtct tgccagttaa caactcattg gcttcaagat agccgatata attgatagaa
    61321 gggattgttt taagcaccgc tgaggcatcc cgaatactgt caagaccctt tacttcttct
    61381 tcaccaatat tgagcaacgc cacgcgagga ttgggaattt ccaccacctc ttcagccaga
    61441 actgagccca taatggcaaa ttgcaccaac attgtgctgt cacaatcgac gttggcccct
    61501 aaatcgagga ccaccgtttt gcccttttgc tgatgtggta ataccgtcac cagcgccgga
    61561 cgctcaatcc cctccagggg cttgagtaat aattttgcca gccccatcag tgccccggta
    61621 ttaccggcac tgacacaggc ttgcgctcga ccttctttca ccagctccag ggccatacgc
    61681 attgaactcc cacggctggc gcggatagct tgcgaaggcc gggcatcact ggcgataact
    61741 gactgcgcag gaataatctg cagacgcgaa cgttgttcaa agtcagcttt agcaagtaat
    61801 ggcgtgatgg cgtcgggatt gccgactaaa agaagagtga gttgcgaatt agaattcagt
    61861 gcctgcaatg ctgcaggcac tgtcacggaa gggccaaaat cccctcccat gacatctaac
    61921 gccagggtta gacgtgtcaa ggtatcgtcg ccgcccggtt tggtctttcc ccagttgcct
    61981 ggggaaaatc ctcactaagc ttcatcacgc agacgcgtga ttacttagcg atgaccttgc
    62041 ggccgcggta gtaaccgtcg gcagtgatgt ggtgacgcag gtgtttttca ccagaagttt
    62101 tgtctacaga caggctggtg actgcggtca gcgcgtcatg ggaacgacgc atgccacgtt
    62161 tggaacgggt tggtttattc tgttgtacgg ccatggacct tactcctcaa ttacggttta
    62221 tccccgatcc aacggggagc accaattact tacgctttaa gctggctaat acggcaaatg
    62281 ggtttggctt ttgcacttct tcaggcagtt caccaaagac catgtccgct tcggacactt
    62341 cacagtgttc agaatcatgc accggaacta ccggcaaggc gaggatgatt tcatcttcaa
    62401 ccattgcaag cagatcgatt tcaccgaatt cgttaacctc aatcggttca tacgcttccg
    62461 gcagtgcttc agcctgttcg tctgaacgca caggactaaa acaatacgtt gtgtagacct
    62521 gatgagtaaa cggcttcccg caacgctgac actcgagcgt taccgtcacc ttcgcatcgc
    62581 cgtttaacac tgcgagacgt tggttatcga tagcgaacga catggagcat tccacatcac
    62641 tgtccacact gactacggat tcggcgacgc gctcaacctg atcaggggta tagataccct
    62701 ggtaatcaag gcgtttttga gccgtacgaa ccggatcgag agtcaggggt aattttacct
    62761 tttgcatagg gcgcgcatat taactttgta acgtcataga gtcaaagaaa aaggcagcct
    62821 gtggttgcct tttgccaata attcgcacac attgcgggtt actgctttat tttcgttcag
    62881 tgctgataac gaacatcaac ctgcaaaacg aagcgcatag tttaaaatgg ctatcatcaa
    62941 tacgctatat ctgatggaaa aaaatatgcc taaacttatt ttagcctcca cctcgccatg
    63001 gcgtcgcgct cttctggaaa aactgcaaat ctcttttgaa tgtgcagcac ctgaggtcga
    63061 cgaaacccca cgcagcgacg aatcaccgcg acagttggtg cttcgactgg cacaagaaaa
    63121 atcgcaatct ctggcgtcac gttatccgga tcatttaatt attggttcag accaggtatg
    63181 tgttcttgat ggcgaaatca ccggtaaacc gttaacggaa gaaaatgccc gtctgcaatt
    63241 acgcaaagcc agcggcaata tcgtcacctt ctatactgga ctggcgttgt ttaattcggc
    63301 gaatgggcat ctacaaacag aagtggagcc ttttgacgtc catttccgtc acctaagcga
    63361 ggcggagatt gataattacg tgcgtaagga gcatcccctg cactgcgcgg gtagctttaa
    63421 gagtgaagga tttggcatta cgctgtttga gcgtttagag ggacgtgacc ctaacacgct
    63481 ggttggttta ccgctaatcg cgctgtgtca gatgttacgt cgggaaggga aaaacccgct
    63541 gatgggatag ttgtttgcgc cggatgcggt tacgtcgcat ccggcaatcg gtgcaggcgg
    63601 cctgatgcgg cgtaaacgcc ttatcaggcc aaccgcccat tatcaggctt atatgcttgt
    63661 attagcgcgc gttacgcagc ttttgcaaac aacgcttcaa accttcatcc atcggcgctt
    63721 cgatacgcat cacctcaccg gtccccggat gggtaaactt caacgctgcg gcgtgcagga
    63781 acagacgatt taatcccgtg cctgcttcag tgagctgtct gtcaaattca cggtcaccgt
    63841 agcgatcgtc aaaggcaatc ggatgacccg catactgtgt atgcacacgg atctgatgag
    63901 tacgtccggt taccgggcta caacgcacca gggtggcaaa tgcatagcgt tcttccactt
    63961 taaagcgtgt ttccgacggt ttgccttcct gactgacacg cacgatacgt tcgccgcttt
    64021 gcagaatatt tttcaataac ggcgcttgaa cgctcttcac atgcgactgc cactgaccgc
    64081 gcaccagcgc caggtaatct ttttgcattc ctttttcacg taattgctca tgcagagaac
    64141 gcaacgccga gcgttttttc gctaccagca acacacctga agtgtcccgg tcgagacgat
    64201 gaaccagttc aaggaaccgc gcttccgggc gcaacgcccg caaaccttcg ataacgccga
    64261 agcttaaacc actgccgcca tgtaccgccg taccggaagg tttattcagc accaggatat
    64321 gatcatcttc atataagatg acgtccgcca gcgccgccac cttttgcaga tgtggcgaaa
    64381 ccgcctcttc ttcccgctca gcaacgcgaa ccggtgggat acgcacctca tcacccgctt
    64441 cgagtttata ttcaggctta atacgttttt tgttcacccg cacttcacct ttacgcaaaa
    64501 tacggtaaat catacttttt ggtacgcctt tcaattgggt acgcaaaaag ttatcgatac
    64561 gttgccccgc ttcgtcagcg gtgatagcaa caatttttac ggatggagtc tctgttttca
    64621 tggtgggcga ttctaattag ccaacaggat tcgcgccact catttttcta tgcttatttt
    64681 tagttttgca ccttattact tcactacgtg atcactttat tgatggttat taaaccaatc
    64741 accagcaaga agtgaaaaaa ctgtgagtaa gcgggtgata aatggtaaaa gtcatcttgc
    64801 tataacaagg cttgcagtgg aataatgagg ccgtttccgt gtccatcctt gttaaaacaa
    64861 gaaattttac ggaataaccc attttgcccg accgatcatc cacgcagcaa tggcgtaaga
    64921 cgtattgatc tttcaggcag ttagcgggct gcgggttgca gtccttaccg gtagatggaa
    64981 atatttctgg agagtaatac ccagtctgtt tctttgataa ttgcgctgtt tttccgcatg
    65041 aaaaacgggc aaccgacact ctgcgcctct ttgagctgac gataaccgtg aggttggcga
    65101 cgcgactaga cacgaggcca tcggttcaca cccggaaagg cgttactttg cccgcagctt
    65161 agtcgtcaat gtaagaataa tgagtaagtt acgatgaaaa gaatgttaat caacgcaact
    65221 cagcaggaag agttgcgcgt tgcccttgta gatgggcagc gtctgtatga cctggatatc
    65281 gaaagtccag ggcacgagca gaaaaaggca aacatctaca aaggtaaaat cacccgcatt
    65341 gaaccgagtc tggaagctgc ttttgttgat tacggcgctg aacgtcacgg tttcctccca
    65401 ctaaaagaaa ttgcccgcga atatttccct gctaactaca gtgctcatgg tcgtcccaac
    65461 attaaagatg tgttgcgtga aggtcaggaa gtcattgttc agatcgataa agaagagcgc
    65521 ggcaacaaag gcgcggcatt aaccaccttt atcagtctgg cgggtagcta tctggttctg
    65581 atgccaaata acccgcgcgc aggtggcatt tctcgccgta tcgaaggcga tgaccgtacc
    65641 gaattaaaag aagcactggc aagccttgaa ctgccggaag gcatggggct tatcgtgcgc
    65701 accgctggcg taggcaaatc tgctgaggcg ctgcaatggg atttaagctt ccgcctgaaa
    65761 cactgggaag ccatcaaaaa agccgctgaa agccgcccgg ctccgttcct gattcatcag
    65821 gagagcaacg taatcgttcg cgccttccgc gattatttac gtcaggatat cggcgaaatc
    65881 cttatcgata acccgaaagt cctcgaactg gcacgtcagc atatcgctgc attaggtcgc
    65941 ccggatttca gcagcaaaat caaactgtac accggcgaga tcccgctgtt cagccactac
    66001 cagatcgagt cgcagatcga gtccgccttc cagcgtgaag tccgtctgcc atccggtggt
    66061 tccattgtta tcgacagcac cgaagcgtta acagccatcg acatcaactc cgcacgcgcg
    66121 acccgcggcg gcgatatcga agaaactgcg tttaacacca acctcgaagc cgccgatgag
    66181 attgctcgtc agctgcgcct gcgtgacctc ggcggcctga ttgttatcga cttcatcgac
    66241 atgacgccag tacgccacca gcgtgcggta gaaaaccgtc tgcgtgaagc ggtgcgtcag
    66301 gaccgtgcgc gtattcaaat cagccatatt tctcgctttg gcctgctgga aatgtcccgc
    66361 cagcgcctga gcccatcact gggtgaatcc agccatcacg tctgcccgcg ctgctccggt
    66421 accggtaccg tgcgtgacaa cgaatcgctg tcgctctcta ttctgcgttt gatcgaagaa
    66481 gaagcgctga aagagaacac ccaggaagtt cacgccattg ttcctgtgcc aatcgcttct
    66541 tatctgctga atgaaaaacg ttctgcggtg aatgcgattg aaacgcgtca ggacggtgtt
    66601 cgctgcgtga ttgtgccaaa cgatcagatg gaaaccccgc actaccacgt gttgcgcgtg
    66661 cgcaaagggg aagaaacgcc gaccttaagc tacatgctgc cgaagctgca tgaagaagcg
    66721 atggcgctgc cgtctgaaga agagttcgct gaacgtaagc gtcctgaaca acctgcactg
    66781 gcaacctttg ccatgccgga tgtaccgcca gcgccaaccc cagctgaacc tgccgcgact
    66841 gtcgtagccc cagcaccgaa agctgcaacg gcaacaccag cagctcctgc acaacctggg
    66901 ctgttaagcc gcttcttcgg cgcactgaaa gcgctgttca gcggtggtga agaagccaaa
    66961 ccgaccgagc aaccaacacc gaaagcagaa gcgaaaccgg aacgtcaaca ggatcgtcgc
    67021 aaacctcgtc agagcaaccg ccgtgaccgt aatgagcgcc gcgacacccg tagtgaacgt
    67081 actgaaggca gcgataatcg cgaagaaaac cgtcgtaatc gtcgccaggc acagcagcaa
    67141 actgccgaga cgcgtgagag ccgtcagcag gctgaggtaa cggaaaaagc acgtaccacc
    67201 gacgagcagc aagtgccgcg tcgtgaacgt agccgccgcc gtaacgatga taaacgtcag
    67261 gcgcaacaag aagcgaaggc gctgaatgtt gaagagcaat ctgttcagga aaccgaacag
    67321 gaagaacgtg tacgtccggt tcagccgcgt cgtaaacagc gtcagctcaa tcagaaagtg
    67381 cgttacgagc aaagcgtagc cgaagaagcg gtagtcgcac cggtggttga agaaactgcc
    67441 gctgccgaac caattgttca ggaagctcca gctccacgca cagaactggt gaaagtcccg
    67501 ctgccagtcg tagcgcaaac tgcaccagaa cagcaagaag agaacaatgc tgataaccgt
    67561 gacaacggtg gcatgccgcg tcgttctcgc cgctcgcctc gtcacctgcg cgtgagtggt
    67621 cagcgtcgtc gtcgctatcg tgacgagcgt tatccaaccc agtcgccaat gccgttgacc
    67681 gtagcgtgcg cgtctccgga actggcctct ggcaaagtct ggatccgcta tccaattgta
    67741 cgtccgcaag atgtacaagt tgaagagcag cgcgaacagg aagaagtaca agtgcagccg
    67801 atggtgactg aggtccctgt cgccgccgct gtcgaacctg ttgttagcgc gccagttgtt
    67861 gaagaaatgg ctgaagtcgt agaagccccc gtaccggttg ccgaaccgca accggaagtg
    67921 gttgaaacga cgcatcctga agtgattgcc gccgcggtaa ctgaacagcc gcaggtgatt
    67981 accgagtctg atgttgccgt agcccaggaa gtcgcagaac acgcagaacc gatggttgaa
    68041 ccgcaggaag agacggcaga tgcagatatt gaagaagttg ccgaaactgc tgaggttgtg
    68101 gttgctgaac ctgaagttgt tgctcaacct gccgcgccag tcgtcgctga agtcgcagca
    68161 gaagttgaaa cggtggccgc ggttgaacca gagatcaccg ttgagcataa ccacgctacc
    68221 gcgccaatga cgcgcgctcc ggcaccggaa tatgttccgg aggcaccgcg tcacagtgac
    68281 tggcagcgcc ctacttttgc cttcgaaggt aaaggtgccg caggtggtca tacggcaacg
    68341 catcatgcct ctgccgctcc tgcgcgtccg caacctgttg agtaataatt agctcaaagt
    68401 aatcaagccc tggtaactgc cagggctttt ttatttcatg tttgaatcaa catattaaaa
    68461 ttcatcaaat ggcatccttg ctaaccaaca atgcaaaata ggcaaaaaaa atgcccgccc
    68521 cggcgatccc agagcgggca gacaaaacat acccagtttc atgatatgtt ttaaagcgag
    68581 cttatttgtt gagctggaag agcgacaatc cctgcatatc ggtaaatgct ttatacgatg
    68641 cctgcaatgc cgtttgctgc atgatgtaag atgaaatagt tgcattccag tccacatcaa
    68701 ccagatcgct catctgctgc gtttgcccta aagcgcgatc gctacctaag gaatccagcg
    68761 actccagttt gttcagctgc gtgcctaatt ccgcgcgaac ggtaaggaca ttattcagcg
    68821 agtttttcag tccacggttg gttttatcca atgccgcagc ggcgatttct ttatccgctt
    68881 cgctatccgc gaccggcgtt ttcagggctg cgatggcgct atccagcatg gcaaaaagat
    68941 tggtttcaga agcgctaccg tctggttccg ctaccgcgtt actggtaata ctgtcgaaaa
    69001 ttttgtcgcc cgtatgtcct atcaccatcg aacgcgaagc atcgacctgt tgcttaatac
    69061 tttctgttcc gccaacataa tcaccgttag cctcactaaa cggcgcagtc tctgttttat
    69121 atgtagcgcc cattaccgtc agtggtgttc gccagattca gcaactggtc acgaagcccc
    69181 tgaatatccg tagccagcga ggcccggtca tcgtcactca aggtgccatt gctggcgtag
    69241 acaatttttt cctgagcatt ctggatagca gtggtgacct ggctaagtac actctcttcc
    69301 agtgacactt tttgagtggc gaaagtacgt gccagcgtgt actggctgtt ttgcgcctgt
    69361 gcctgggaga gaacaacagc ttgtgatgca gcaatgggat cgtctgaagg gttaacgact
    69421 cgcttacccg tcgacatctg ctcgccgtac ttcatccatt ctgcctgaga attggtgatg
    69481 ccgcgcatgt tttgctggta catcatctgg gtactgaaac gcattttatc tccccttagc
    69541 gaatgttaat cagcgcatca aaaatcgcgt ttgccgtctg cagaacctgc gcattcgcca
    69601 ggtaatactg ctgaaaacgt tgcagatttc cgtactcctc atcgagattg acaccggaaa
    69661 tcgactgctg ctgattggaa agctgcgtca ccacattacc ttgcgtggtg ctactggttt
    69721 tcaacgtcgc ggttttatta ccgatatcac tcactaacga ggcataagcg tcgttaaagg
    69781 atttcgcacc gcccaccgtt ttactgttgc tttgcagatc cagcagggcc tgaccgttgc
    69841 ggttgtcgct atcacccgca tcttcttcgc tcgccatcgc gattttcgct tcgtcggtga
    69901 ttaatacatc catgttgacg atggcgtcac ttactggttt cagcgtgaag ctgtcgttaa
    69961 cggcaggcgt tcctgtaaac gtcaactcca gaccatcaaa tgccacttta ccgttggcat
    70021 ctggtgtcgc cgtaaaagtg gtattgctgg caaggcgggt gacctgccac tgattattat
    70081 cgaacgagat tttgtaatct gtcgccagta ccacggaggc atcagttacc gtggcaccga
    70141 tcgcaacgtc acctttgttt ttcgtgtttt gcagaaccgc gggcttaccg atagtaaaga
    70201 aatcttcacc agcatcgccg ttagcatcaa atccggcttt gtgttggctg ttgaaagcct
    70261 cggcaaatgc cagcgccagt tgtccaagcg tattacgcgt ctggtccaga tcctgagaac
    70321 ggaatgtcag aatgccgccc agcgacccgg tattcagtaa tttctccggg atctcaatat
    70381 tgcctgccgt cccatcaacg taagcgacag tcgtacgaga agggtcggcg ctggaaggaa
    70441 ctgccgccag ttgccgcgcc gtacttccct gaaccagtga gtaaccattg gcatcgtgat
    70501 gttataagta ccgccatcct gaacgctgac ttctacgcca acaatctggt ttaattcgct
    70561 caccagttga tcgcgttgat ccagcagatt gttaggtgac gcccctgccc ccacgcctgt
    70621 caggcgcgag atttgatcgt tcaggctggc aatttgttta gcgtaattgt tgatctgatc
    70681 aacgctggca cctatcgcga tattgacctg tttgtcctgg tcgcgcagat attgatcggt
    70741 ggttttaaac tgattcacca acccttctga tttcccaatc agcgcctggc gcgccgccgg
    70801 gtcttccgcg ttactcacca gcgtttgcag gctggtgaag aaatcctgca tctgtgttgc
    70861 cagcgaagag gtactggtgg agagcatatt gtcgattttc gacatctgct catagcgggc
    70921 ggtcaggccg ctactttgcg tctgcgcagc acgtaactgg ttggtaataa acgtatcata
    70981 ctcacgctgc acaccagaaa cgtagacgcc attgccaacc cagccgccag cgcccaacgt
    71041 gctattggcc tgcgccataa tagtggtttg gcgggtatat ccggcaacgt tatagctgga
    71101 gatattatta cttgccgtat ttaacgccgc ctgggccgcg ttcagtccgc tcatggcgtt
    71161 attgatcaag ctggacatgg aggttcctta taagccttca attacagagt attatcggca
    71221 gcgacccgcc ggacttgagt tattcagaac agattatcaa tgttcatgct gtaggttttg
    71281 ctcaccttgt cgcttatcga tttcatctgc tggatcatgt tggtgagttt acgggcatag
    71341 tgaggatcgg tagcatagcc cgcgtcctgt aggacctgcg ccccctgttc cgcactcgcg
    71401 gcggtcgtca cggcggcgta gcgcgggtta cgtgttaaca gcccaacgta atccgacaat
    71461 gcttccagat acgagctgta gacccgaaac ttcgctttta ctttcttcgc ttcgccgttt
    71521 tcatattcag tcgtggtgat ttcagtgact tgccctttcc agttgccaga ggctttgaca
    71581 ccaaacaggt tatagcttgg ctcgccgttt tcgcggcgga tttgccgttg cccccaacca
    71641 gattcaagcg cagcctgagc gaggatcaaa tgatgtggca caccgctttg ctggcttgcc
    71701 agttgggcgg gcagcgagag ttgcgcgagg aatgctctac tgtcacccgg cagcgaatca
    71761 tcgtagttac gtggtacggc cttttgcacc agctgcgaaa gcgtctggtt ttgataacgc
    71821 accacggttt cgagcgggaa tttcatcggt gctgctggcg tggactcctc tggcaatggt
    71881 tgttctggcg tcatctgttt aaccatcatc tctgcaagcc ccagaccttt gcccgccgtc
    71941 atctgttggg caatttgctg gtcatacata ctggtataca gacgagtgtg ctcgctgctg
    72001 aacaggccat cttttggtaa agcgtcgcgc atgcttttca acatcatctg cacgaacatc
    72061 ccttccacct gacgggccac cggacggata tttgccgccg gatcttcgct cgctttcgcc
    72121 tttagttcgt tgagtgattg cgcatcccag gccgcacttg ccagtagttt gctgtcgctg
    72181 atcatcagat gatttccagt tttgcccgca gacatcccgc actttgcatt gattgcaaaa
    72241 tagacatcag atccatcggc gtagcgccca gcgcattgag cgcacgcacc acgttattga
    72301 ggctggcgct ggaacgtacg ctttgcagcg aaccgccgct ctggcgtaaa tcgatctgcg
    72361 tttgtggagt aacaacagtc tgtccaccac caaacggtgt atctggctgg ctgacattgg
    72421 cctgacgatt aactgttact gagagatttc cctgcgctac cgcgcagctg tcgagggtca
    72481 cttcgcgatt catcaccacc gaaccggtgc gcgagttaat cactacttta gcgtcctgcg
    72541 gggtgacatt aacctgcata ttctggatat cggcaaggaa acggacctgg gaactgttgc
    72601 cacttggtac gcgcacctga atagtccgcg catctaacgc ggtggcgctg ccatatccac
    72661 gcacgcggtt gatggtgtca gcgatttgct gcgccatgct gaaatcttcg tcgttaagtt
    72721 gcaaattaag ggtattcccg acgccaaact ggctgggcaa ttcacgttca ataaccgcac
    72781 cattggtgat ccgtccaccg ttcagttggt taacctgaac actgctaccg cctgcggagg
    72841 ctcctgcgcc gccaaccaga atattgccct gcgccagcgc atacacctga ctgtcaacgc
    72901 ccttaagcgg agtcatcaac aacgtacctc cacgcaagct tttggcatta cccatggaag
    72961 aaaccaccac atcgatggtt tgcccctgac gtccaaacgg aggaagtgac gctgtcacca
    73021 ttaccgcagc gacgtttttt agctgcatat tggtgcccgt cggaacggta attcccagct
    73081 gtgagagcat gttattaagc gtttgtgtgg taaacggcgt ctgggttgtc tggtcaccag
    73141 tgccatccag ccccactacc aagccatagc caatcagtga gttttgcctt accccctgaa
    73201 cactggtgag atcgcgaata cgctcagcct gagccgccgt cgtaaccagt agaagaatta
    73261 atgcagagag aaatttaatc accacagcct cacttacatt ggcgacaggt taaggaagaa
    73321 acgctgcaac cagcccatat tttgcgcttc gttaatgtag ccattgccta cgtattcaat
    73381 acgcgcatcc gccacctgag tagacggtac ggtattgctg ccgctgatag tgcgtggatt
    73441 aaccacgccc gagaagcgaa taaattcggt accctgatta atggcaatct gtttttcacc
    73501 caccacatgc aggttgccgt tgaccagtac ctggtcaacc gtcaccgtca acgtgccgct
    73561 aaaggtattg ctggcattgg ccccgccctt tccgttgaac gtgttaccac cggaggcttc
    73621 gacatcggca cgagcgttac caaacagccc ctgcaaatag cgcggcacag tatcaaagcc
    73681 aaaattagtt ttaccgtcac ggctggcatt cgcagaggag cttttgctgg cgctgacgtt
    73741 ctcctgcaac acgatggtca gcgtatcgcc aatattgcgt ggtcgacgat cttcaaacag
    73801 cggttgatag ccatagttaa tcggctgagc agactggaaa atagaaccgt tggcgacggg
    73861 cgtcggaccg ggaaccggtt gtgcactggt cgccccctgc accagcggcg tggagggtat
    73921 ccaggcgcag ccggttagtg aaagcaccaa caagctggaa atggcataag tatgcgcagc
    73981 gtttttttgc atggctatca tcttcaaaaa tcagtaaacc ggtgaacctg ccaccggtta
    74041 tgccttagag ttgcgtcagt ttttgcagca tctgatcggt ggtggacacc gctttactgt
    74101 tgatttcgta agcgcgttgc acctgaatca tattgaccag ttcttccgcc acgttgacgt
    74161 tagacgtttc aacataccct tgatacagca gtcccgcgcc gttcaggccc ggcgtgcttt
    74221 cgttcggtgc accggaggat tgcgtttcgg tgtagaggtt ttcgccaatg ctctccagtc
    74281 cggtatcatt cataaaggtg gtgagattga gctgcccaac ctgaaccgga gctgcctggc
    74341 cttgttgggt tacgctgacc acgccatcac gaccgatggt gatacttaac gcattcgccg
    74401 gaatggtgat cgctggctgc acctgaaaac caccagccgt caccagctgc ccgttctgat
    74461 ccacctggaa agagccgtca cggatatagg ctgatgagcc atctggcaac attacctgga
    74521 aaaagccctg ccctttaatc gcgacatctt tgctgttatt ggtctgcgac agattgccct
    74581 ggctgtgtaa gcgttcagtg gcgaccgggc gtacccccgt gccgatttgt aatccggagg
    74641 gtaaggtggt ttgttcggaa gactgtgccc ccggctggcg aatggtttga taaagcagat
    74701 cttcaaacac cgcgcgctga cgcttaaaac cgttagtact gacgtttgcc aggttgttgg
    74761 caatgacgtc catattggtt tgctgggcgt caaggcccgt tttggcgatc cataatgaac
    74821 tgatcatata atgtcctgta gtggattgct gacaagcaga gcagaagcac gcgacagtgt
    74881 gaatttcccc tcaccctaac cctctcccca aaggggcgag ggaatagtcc gcgcgctttt
    74941 attgcaatgt catcaatcgt tcaattaact catcgacagc agttggttgg cacggcctgc
    75001 gttatcatcg acgctgctga tcaccttcat ctgcatttca aaacgccgcg cgctggcaat
    75061 catgtcgctc attgccgcaa cggcattgac gttactgcct tccagaaccc ccgacatcac
    75121 acgcaaggtt ggatctgcct gcagtaccgg ccctcgcgtg gcctgggttt ctgcgcttaa
    75181 acgaaaaatg ccgtcgtcac cgcgctgcac ttcgctgccc gtggctttca ccagtttcag
    75241 acgccctact ggcgcaactg tatttgccgg atcgcccgga ttgagcgccg agattgtgcc
    75301 atcggcagca atcgtgattt ccgccccttc cggcacagca attggccccg cctcgcctat
    75361 caccggatgc ccctgaattg tcagttgccc ggtgggatca acctgaatgc tgccattacg
    75421 cgtataccct tcgctgccgt cagcggtctg cacggccagc catcctgctg caacgcaacg
    75481 tccagcgggc gagaggtgta atccatcttg ccgggcgtca tgtctgcacc cggtgttgac
    75541 gccgttacca gcgtgcgcgt gggcagtgaa agcccttcca ccggcactgc gcgcaaggcg
    75601 tttaactgcg cgcgaaaacc gggcgtagag gcattggcca gattactggc ggttaccgcc
    75661 tgttgattca gcgtctggct ggctgctccc atcgcagtat aaattgcgtg atccattgag
    75721 ctatcccgtc agcgattagc gtaagttaac ccgcgtgttg aggatctggt cctgggtttt
    75781 gatggtctgg gcgttagact tatagttacg ctgggcaacg atcatattga ccagttcttt
    75841 actgagatcg acgttggacg cttccagcgc accgttggtc agagtgccaa agtttcccgt
    75901 cccggctgtc cccaacagcg ccacgccaga agattgcgtc gcagaccaga cgttgtcgcc
    75961 ttcggatgcc agaccttcgt tgttggcaaa gttcgccagt acaatctgcc ccagcagttg
    76021 ggtttgttcg ttggaattgt tgccgacaac cgtaccgtca tcattgattt gataactcac
    76081 cagatcgccc ggtttgtagc cattctgggt ggttgccaca atgttgttag cgccggttgc
    76141 gatattattc gccatcgcac catccactaa tgtgccatta gcattaaatt ccagtgttgt
    76201 cgctgtcttc gcaatgctgt ttggatcact gctatcctgg gtgtagacct gccagttatt
    76261 atccccggtc ttcacaaaat agacgctcat gtcatgagca ttaccctgac tgtcgaaaac
    76321 agtcaccgaa ccttttttgt tatagctatc cgcattgctg gcgctgaatg gcgtaacagt
    76381 aggaagcgga tcactggaat tcaggttgat ctgcatcgac gccgtggtgg tagttttcgc
    76441 tgccatcagg gtattcggga tcgaaatatt ggtcggattc gccccttgct gaatagtcgg
    76501 cggcgtaccg gttaccgggt aacccgtcag ctgtaaacct tgcgtattca acaggttacg
    76561 gttttcatcc agcttaaatt gtccgttacg gctgtagaac accgaaccgt tgctgtctac
    76621 cagacggaaa aaaccgttct ggctgatagc aacgtccaga cctcgcccgg tgttggtggt
    76681 cgtgccatcg gtaaagtcct gagtgatacc ggcaactttt acccccagtc ccactttcga
    76741 accggcaaac atatcggcaa aagaggccgt gcctgattta aagccgtagg tggcggagtt
    76801 ggcgatattg ttgccaataa catcgaggtt ggtggcggca gcgtttaatc cgctaaccgc
    76861 ttgagaaaag gccatgactg actcctgaaa tgtgaaggct taaattatct gccgtacttc
    76921 gtcgagggtg gtggtgccgt aagtgccgag atccagcgta ttaccgctgt tgccgcggat
    76981 cacaccctgc accagagcaa actgcagcgg ctgggcaacc agttgtgtac caccgttact
    77041 ggcgctaatc gctacattgt aagaaccgtt cggcgcagtg ctgccatcag tcaacgaacc
    77101 gtcccaggtg aaactgtgaa ctccggcggt cagttcacca atatcaatgg tgcgcacaac
    77161 cgcgccattt ttatcggtga tggtggccgt aactttgtct gccgcctgtt gcagctcaac
    77221 accaaacggc gtggtcgtgg tcacagcccc ttcttcactg ccggttcccg caagaacagt
    77281 tgtgccgggg atcatcacgc cgtgaccgat caggttactg gcctgtaacg actggctgtt
    77341 atcaatctgt ccggaaatag atccgagcgt ggtattgagt ttttcaatcc cgctgaccgt
    77401 gctgatttgt gccaattgcg acgtcagctc gttgttttcc attggattgg tcgggtcctg
    77461 gtttttcagc tgcgccacca gcaaagtcag aaaactgctt tgtaaatctg cggcgttgct
    77521 gcccgtgagc gaactactgc tggtggtact gacgccggta tttgtcggat cggtggtggt
    77581 taccgcaatg gacatagctt tctcctttat tgaccgagcg taagggtttt cagcatcatg
    77641 cttttcaccg tgttgagcac ttcaacattg gcctgatagc tgcgtgacgc cgacatggtg
    77701 ttaaccatct ccccgacgac atcaacgttc ggcattttta tgtagccctt tgcatctgcc
    77761 agcggattac ctggttcata aaccagtttg tccggggcct gactttctta acatcggcaa
    77821 cctttacgcc gcctgtcgca gcacctggtg cagcgttaac ctggaatacc acctgttttg
    77881 cccgatatgg ctgtccatca ggaccggtca cgctatcagc atttgccaga ttactggccg
    77941 ccacgttcag gcgctgggac tgggcagtta acgccgaccc ggcgatatca aaaatattca
    78001 gcagtgccat ccgttaattt ccgctctgta aaacgttcat catgcctttg atttgcccgc
    78061 tcaacgcgct aaggctcatc tggtattgca ggctgttatc ggcaaactgg gtgcgttcgc
    78121 gatccatatc gacggtatta ccgtcaagcg aaggctggtc cggaatacgg tattgcagtt
    78181 ctgcggtagg aggcgtcagc gcctgcgccg ggatgtgctg cgttgaggtc atcgtcagtg
    78241 caaccacact ggttgcatcc cgtccacgtt gcatgacttt tttaagttca ctggcaaaat
    78301 cgatatcgcg cgcctgataa ccaggggtat cggcattggc gatgtttgct gccagcactt
    78361 cctgacgctg ggcgcgcaga ttgagcgcct cttgttgaaa acgtaaggcg gcgtcgagct
    78421 tatcgagcat atctcctccg caggtatcaa aattctgcca tcagcttaaa tgcctttacg
    78481 cgcgcgttat cggcggaata aacgcaaaat gggtcgctat ttatgccgtt gatggtcatt
    78541 gcggacaggt acaattcacg ttgtagaaat ggctgggggc gaaaatgctg acaataaaac
    78601 gtagcgtggc gatcatcgcg atactgttca gtccgttaag tacggcgagc aatctcacat
    78661 cgcaattgca caactttttt agcgcccaac tcgcgggggt aagtgatgag gttcgtgttt
    78721 ctattcgtac agcgcccaat ctactaccgc catgcgagca gccattgctt tcgatgagca
    78781 ataattcccg cctgtggggc aatgtgaatg tgttggcacg ctgcggtaac gacaaacgat
    78841 atttacaggt taatgtgcag gccacaggaa attatgtggt tgccgcgatg cctattgtgc
    78901 ggggaggaaa gctggaagct ggcaatgtca aactgaaacg tggacgcctg gataccctgc
    78961 caccgcgtac ggtgctggat atcaatcaac ttgttgatgc cgttagcctg cgcgatctat
    79021 cacccgatca acctatccag ttaacccagt ttcgccaggc atggcgggta aaagcgggac
    79081 aacgcgtcaa tgtgatcgcc agcggtgatg ggtttagcgc caacgcagaa ggtcaggcgc
    79141 tgaacaatgc agccgtcgca cagaatgcgc gggtgcgcat ggtatcggaa caggtagtca
    79201 gcggcgttgt tgatgcagat gggaatattc ttataaacct gtaagctgtt aaagattacc
    79261 cgtcccttgc cgataaataa gcaacacatg ataaaagcgc cctcaatgag gaataaacca
    79321 tgagtattga tcgcacttcg cctctgaagc ctgtaagcac cgttcaaccg cgcgaaacca
    79381 ccgacgcgcc ggtaacgaac agccgggcgg caaaaacaac cgcctccacc agcaccagtg
    79441 tgacgttaag cgacgcgcaa gcaaaactga tgcaacccgg cagcagtgat atcaatcttg
    79501 aacgtgtcga agcgttaaaa ctggcgattc gtaacggtga actaaaaatg gacaccggca
    79561 aaattgccga tgcgctgatc aacgaagcgc agcaagactt gcagagtaac tgatcgtatg
    79621 acacgtcttg cagagatcct cgaccagatg tccgctgtgc ttaacgatct caaaacggta
    79681 atggatcaag agcagcaaca tctctctatg gggcagatca acggcagcca gttgcaatgg
    79741 attacagaac aaaaaagctc actgctggcg acgctggatt acctcgaaca gttacgcaag
    79801 aaagaaccca atacagcaaa tagcgttgat attagtcaac gctggcagga aattactgtg
    79861 aaaacgcagc aactacgcca aatgaatcaa cataacggct ggttactgga aggacagatt
    79921 gagcgcaatc aacaggcgct ggaaatgttg aaaccgcatc aggaaccgac gctatatggg
    79981 gcgaacggtc agacctcaac accccatcgc ggcagtaaaa agatttcgat ctgaatatta
    80041 tctccggcct gcactgcagg ccggaatgca ttgttacacc gtccggcggg caaattcttt
    80101 aactttgaag cccagtaccg ccagtgcagc gaagtacgcg gcaatccccg ccagcacgac
    80161 cgccattaaa cgcagtaagc gccagggcat ggtacccaat gaccactccg gcatgatatg
    80221 taacatacct aaaagcacgc cagacatcac cagtaccgcc accaccagac gcaacagaaa
    80281 cgccatccag ccgggttgcg gagtaaagat tttctgctta cgcaactgcc agtaaagcag
    80341 cgaagcattc agacacgccg ccagaccaat agaaagtgac agcccggcat gtttcaacgg
    80401 accaataaac gccaggttca tcaattgcgt taaaatcagc gtaacgatgg caattttcac
    80461 tggcgtttta atgtcctggc gggaataaaa gccaggagcc aacactttca ctacaatcag
    80521 gccaatcaaa cccaccgagt aggcaattaa cgcccgctgg gtcatcagcg catcaaacgc
    80581 ggtaaattta ccgtactgga acagcgaaac ggttaacgga cctgaaagaa tgcccaacgc
    80641 aaccgcactc ggcagtgcca acaggaaaca aagacgcaac ccccagtcca tcaaacggtt
    80701 gtattcatca tgattgccac tggcaaaact tttcgacagc gacggcagca aaatggtgcc
    80761 aagcgccacg cccagcacac cggagggaaa ctccattaag cggtcggcgt aatacatcca
    80821 agacaccgaa ccggaagcaa gaaacgaggc aaaaatggtg ttgatgatta aggagatctg
    80881 gctcacagag acgccaagga tcgccggtcc catctgtttc accacgcgca ttgctccagc
    80941 atcgtggaag ttaatgcgcg gcaggaccag catgccgatc ttcttcaggt gcggtagctg
    81001 atacaccagc tgcagaatgc cgccgaccgt aacagcccac gccagcgcca gcaccggtgg
    81061 gttaaagtac ggtgcggcaa acagcgcgaa accaatcatg ctgatgttaa gcagtgttgg
    81121 cgcaaacgcc ggaatcgaga agcggttcca cgtattcaga atcgctccca ccagcgacgc
    81181 cagggagatc agcaagatat agggaaaggt aatctttagt agctggctgg tcagggcaaa
    81241 tttgtcagct gtgtcagcga agcctggcgc ggtcaccatg atcacccacg gtgcggcgag
    81301 catgccagcg accgtcacaa ccgccagcgc aagtgtcagc aggccagaaa cataagagac
    81361 aaagacccgc gtggcgtctt caccctgctt acttttatat tccgccagaa tcggtacaaa
    81421 tgcctgggaa aatgcccctt cggcaaagat acggcgtaac aagttaggaa gtttaaaagc
    81481 gacgaaaaag gcgtcggttg ccatccctgc gccaaagatt ctggcgacaa ttgcgtctcg
    81541 tgcgaagcca agcacacgcg aaaacatggt catcgagctg acggcggcca gcgattttaa
    81601 taaattcatc ggtgttctaa tccggaccca cgggcaaaac gcctgcaagg caggcgctga
    81661 aagaggcgaa tagtctacct agattatggt gaattgctac cgccagatgt tacagggttt
    81721 attcactcat cgcatcgcgc cagatcttgt caacgatacg ttgcgccagc actgcctatt
    81781 cgccggcggt ttgcggaact gtctgatttt acacgcattc aatgaagtgc cgcgcacagc
    81841 cgacaaatcc acgttgctca agagtgctct gccaaccagg aatcggttta ttcactacac
    81901 cctgcccgcg ctcctcacgc cattcgcgca tatccgtaat gtcgatgagc gcaccgtcag
    81961 tcacggcctg cacggtttca cgctgacttc cggcacggcg atgcatacaa gtggtgatct
    82021 gcaaaggacc ggccgaaaaa tggtgctcgg caaacagcat ttcgccagcg tcgttagtca
    82081 gtagcgtacc gccatccaga gaggctttgc cgcccgacaa ccacagcgcg gtatccacca
    82141 catgcagata atcatccagc aacgtgaaat aaagatcgtg tggcccgacg ctattgctac
    82201 gatgtttatc cattcttagc gaggctgcgg tggcgagttg cgtttttaac tcaccgtaga
    82261 gtggtgcgaa acgacggtta aaaccgacca tcaacgtcag ttttttccgc gccgccagtt
    82321 ccaccagcct ttcagcatcg cacagatttt ctgccagcgg tttatcgaca cagacatgta
    82381 cccccgcatt gagtaacgta ctgaccacgt caaagtggct ggcggtgctg gagtgcacaa
    82441 aaaccgcatc acaactggcg gcaaggctgg ataacgaatc ggcataagga atgcgccagc
    82501 tgtcacaaat tggcagggct ttggcgcgcg taggcgacca ggctccttgt aacgtccagt
    82561 cagacgctgc cgccagcacc ggtaaccacg ctttttgcgc aatgccgcct aatcccacta
    82621 cgccgatacg taattttttc actttaatct cccagatggg ccagcaacga atcaagacgt
    82681 tgtttcagtt ctgccacttc gatttccagg gcttcgacgc gggtctgtaa atcaccgtca
    82741 accatgttcg acatagccgt taccgccggc tgatcttcaa cctcaccact gaaaagatgc
    82801 atgtagcggc tttcgcgttt acccggttcg cgggccagac gcaccacaaa aggaccatct
    82861 tcgcgattcg ccagttgttc cagcgtcgac tccacttccg ccatatcgct gaattcatac
    82921 attcgcgcgg cgcggctgcg cagttcaccc ggcgtctggg caccacgcaa taacaacgtg
    82981 gtgattaacg ccacttctgc ggcgctcagt ttcagatcgc caaattctga attacaaaaa
    83041 cgttgctcat atttggtgac ccgattacca aaaccgctca ctgtgcgtag ataatgacgt
    83101 ttgaccagat tatccagctg ttcctgcact tcggattcac tcagattcat gaccggttca
    83161 cggttcgttt tctgattaca ggccgtgact acaccattga ctgagagcgg gtattgctcc
    83221 ggcgtggtca cctgcttttc cagcaagcaa ccaattacgc gagcttccag ggcagtaagt
    83281 tgatatttca tgagattctc cttagcggcc gggcgtccag tctggggtag ttaatgccgt
    83341 cagtacgtga tcgcgccatt gtccatcaat caacagatag tctttcgcat agccttcttt
    83401 ttcaaaaccc agtcgcgcca gtaaatcacc gctgcgttta ttgtgcggca tataattagc
    83461 cataatgcga tgaatatgtt gggtgcgctg catataacga atggctgcgg tcagggcttc
    83521 aaacatgagt cctttgccct gccatttttg cccaatcgaa taaccgagat agcaggcatg
    83581 aaaagagccg cgaacaacat tggaaaaatt ggcaacgcca ataatctctt tttcgtccgg
    83641 gtcgaataag ccaaagtaga aagctgaacc ttgtttatga aattcgttaa tcatccctag
    83701 cctggcctgc cagcctgatg gataacagtg gctttcgtcg cgcactggct cccagggctt
    83761 gaggaaatgg cgattctctg cgtaataatc cgccagacgc caggcatcac gatcatgcac
    83821 cagacgcacg accagtcggt ctgtggttaa gcgcactttt ggcacgttac tgcgatagcc
    83881 aaacattcta tacctactcc ttcccgtaac gtctttaata cgctgtaagt attactatac
    83941 ctgcgcaacc agaccctgtg aaaagtccaa aatgccattt tctgcattat tcacatattt
    84001 gataaaaact ctctatcaaa gcgacgtttt gataaaaaaa tactgtcccc gaagtgatgg
    84061 gaaaagtctt agcggcaatg cagaatagaa agtgctcatc ttttatttcc cgggagggga
    84121 aatgtcccgc gtatcgcagg cgaggaacct gggtaaatat ttcctgctca tcgataatat
    84181 gctggtcgtg ctggggttct ttgttgtctt cccgctgatc tctatccgct tcgttgatca
    84241 aatgggctgg gccgccgtca tggtcggtat tgctctcggt ctacgccagt ttattcagca
    84301 aggtctgggt attttcggcg gcgcaattgc cgaccgcttt ggtgccaaac cgatgattgt
    84361 taccggtatg ctgatgcgcg ccgccggatt cgccacaatg ggtatcgccc acgaaccgtg
    84421 gctattgtgg ttttcatgcc tgctctcggg actcggcggc acgttgtttg atccgccgcg
    84481 ttcggcgctg gtggtgaaac taatccgtcc acaacaacgt ggtcgttttt tctcgctgtt
    84541 gatgatgcag gacagtgcca gtgcggtcat tggcgcattg ttggggagct ggctgttgca
    84601 atacgacttt cgcctggtct gcgccacagg ggccgttttg tttgtgctat gtgcggcgtt
    84661 caatgcgtgg ttgttaccgg catggaaact ctccaccgta cgcacgcccg ttcgcgaagg
    84721 catgacccgc gtgatgcgtg acaagcgttt tgtcacctat gtcctgacgc tggcgggtta
    84781 ctacatgctg gctgtacaag tgatgctgat gctgccaatt atggtcaacg acgtggctgg
    84841 cgcgccctct gccgttaaat ggatgtatgc cattgaagcg tgtctgtcgt taacgttgct
    84901 ctaccctatc gcccgctgga gtgaaaagca ttttcgtctg gaacaccggt tgatggctgg
    84961 gctgttgata atgtcattaa gcatgatgcc ggtgggcatg gtcagcggcc tacaacaact
    85021 tttcaccctg atttgtctgt tttatatcgg gtcgatcatt gccgagcctg cgcgtgaaac
    85081 cttaagtgct tcgctggcgg acgcaagagc tcgcggcagc tatatggggt ttagccgtct
    85141 gggtctggcg attggcggcg ctattggtta tatcggtggt ggctggctgt ttgacctggg
    85201 caaatcggcg caccagccag agcttccgtg gatgatgctg ggaattattg gcatcttcac
    85261 tttccttgcg ctgggttggc agtttagtca gaaacgcgcc gcgcgtcgtt tgctggaacg
    85321 cgacgcctga tttgatactc ccccctgact cctccatact gaaagtgaac tgacagccaa
    85381 atggaggagt cacgtgaagc tatacattta cgatcactgc ccttactgcc tcaaagcccg
    85441 catgattttc ggcctgaaaa atatccccgt cgaattacat gttctgctca acgacgacgc
    85501 agaaacaccc acccggatgg tcggtcaaaa acaggttccc attctgcaaa aagatgacag
    85561 ccgctatatg ccagaaagca tggatatcgt tcactatgtc gataaactcg acggcaaacc
    85621 gttactgacc ggcaaacgtt cccctgccat tgaagagtgg ctgcgcaagg tcaatggcta
    85681 cgtcaacaaa ctgctgttgc cgcgttttgc caaatcggca tttgatgagt tttctactcc
    85741 cgccgcgcgc aaatatttcg tcgacaagaa agaggccagc gcgggtaatt ttgccgacct
    85801 gctggcccac tctgacggtc tgattaagaa tatcagcgat gatttgcgtg cgctggacaa
    85861 actgatcgtc aaaccgaacg ccgtgaatgg cgaactttcg gaagatgata ttcagctatt
    85921 tccgctactg cgtaatctga cgctggtagc cggaatcaac tggccaagcc gcgttgctaa
    85981 ttaccgcgat aatatggcga aacagacaca aatcaatttg ttatcatcaa tggcgattta
    86041 atcctgaccc gccgcggcag cctgtattcg cgggttgccg ccgttttcag ttttttctgc
    86101 tggcgagttt atcgctggtg acacatgctg cgttgtgtca ggatattgac gcagatttca
    86161 tgtcgaggac taccatgaac aagtttttat ttgccgctgc attgatcgtc agtggcctgc
    86221 tcgttggctg taatcaactc acccaataca ccatcaccga acaagaaatt aaccagtcgc
    86281 ttgcgaaaca taataatttc tcaaaagata tcggtttacc cggcgtggct gacgcccata
    86341 ttgttctgac aaacttgacc agccaaattg gtcgcgaaga gccgaataag gttaccctaa
    86401 ccggagacgc caatctggac atgaactccc tgttcggtag tcagaaagcg accatgaaac
    86461 tgaagctgaa agcgctgccc gtgtttgata aagaaaaagg tgcgatcttc ctgaaagaga
    86521 tggaagtggt cgatgcgacg gtacaaccgg aaaaaatgca aacggtgatg caaacgttgc
    86581 ttccctattt gaaccaggca ttacgcaatt actttaacca gcaacctgct tacgtcctgc
    86641 gcgaagatgg cagtcagggc gaagcaatgg cgaaaaaact ggcgaaaggc attgaagtga
    86701 agccaggcga aattgtcatt ccgtttacgg attaatgacg agggcgcatt cgcgcccttt
    86761 atttttcgtg caaaggaaaa cgtttccgct tatcctttgt gtccggcaaa aacatccctt
    86821 cagccggagc atagagatta atgactgcac catcccaggt attaaagatc cgccgcccag
    86881 acgactggca ccttcacctc cgcgatggcg acatgttaaa aactgtcgtg ccgtatacca
    86941 gcgaaattta tggacgggct atcgtaatgc ccaatctggc tccgcccgtg accacagttg
    87001 aggctgccgt ggcgtatcgc caacgtattc ttgacgccgt acctgccggg cacgatttca
    87061 ccccattgat gacctgttat ttaacagatt cgctggatcc taatgagctg gagcgcggat
    87121 ttaatgaagc cgtgttcacc gctgcaaaac tttacccggc aaacgcaacc actaactcca
    87181 gccacggcgt gacgtcaatt gacgcaatca tgccggtact tgagcgcatg gaaaaaatcg
    87241 gtatgccgct actggtgcat ggtgaagtga cacatgcaga tatcgacatt tttgatcgtg
    87301 aagcgcgctt tatagaaagc gtgatggaac ctctgcgcca gcgcctgact gcgctgaaag
    87361 tcgtttttga gcacatcacc accaaagatg ctgccgacta tgtccgtgac ggaaatgaac
    87421 ggctggctgc caccatcact ccgcagcatc tgatgtttaa ccgcaaccat atgctggttg
    87481 gtggcgtgcg tccgcacctg tattgtttac ccatcctcaa acgcaatatt caccaacagg
    87541 cattgcgtga actggtcgcc agcggtttta atcgagtatt cctcggtacg gattctgcgc
    87601 cacatgcacg tcatcgcaaa gagagcagtt gcggctgcgc gggctgcttc aacgccccaa
    87661 ccgcgctggg cagttacgct accgtctttg aagagatgaa tgctctgcag cactttgaag
    87721 cattctgttc tgtaaacggc ccgcagttct atggcttgcc ggtcaacgac acattcatcg
    87781 aactggtacg tgaagagcaa caggttgctg aaagcatcgc actgactgat gacaccctgg
    87841 tgccattcct cgccggggaa acggtacgct ggtccgttaa acaataaaaa tcgtagcttc
    87901 ctgttgtcat taggttattt tacctgtata aataaccagt atattcaaca gggggctatt
    87961 atgcgaattg aagtcaccat agcgaaaact tctccattgc cagctggggc tattgacgcc
    88021 ctggctggcg aactttcccg ccgtattcag tatgcgtttc ctgataatga aggccacgta
    88081 tcggtacgtt atgccgcagc gaataattta tcggttattg gcgcaacaaa agaagataaa
    88141 cagcgcatta gcgaaattct ccaggaaacg tgggaaagcg ccgatgactg gtttgtcagc
    88201 gaataatatg cagtgatttt tgtttttgcc ggatcgcccc ggctttttta ttttacttac
    88261 gaattttgag tacaaaattt ctacacttcc tcaaaataat tgcacaatat gctaaaaaaa
    88321 tatgaatgac atgctgtttt attgttcata taaatcttaa aagcagaaat ttatctgttc
    88381 ccccctttaa aaattcggtg aataccctta cttattggta tactgaagtt gcttcagata
    88441 aaaacgcatt gaacctcgaa taacgttgtc tagtaacacg aattaggggg ccatgatgga
    88501 aaaaaataat gaagtcattc agactcatcc gctcgtaggg tgggacatca gcaccgttga
    88561 tagctatgat gcgctgatgt tgcgtttgca ctaccagacc ccaaataagt ccgagcagga
    88621 agggactgaa gttggtcaga cgctctggtt aaccactgat gttgccagac aatttatttc
    88681 gatattagaa gcaggaatcg ccaaaattga atccggtgat ttcccggtaa acgagtatcg
    88741 gcgtcattaa catgacatga ccatccaccc aaaaggcacc tcaccggtgc ctttaccatt
    88801 tctgggttgt atatctgcat tcagttaatt actctgctta aaccatgaga gcagagagaa
    88861 aaaaatgaaa tacgatctca tcattattgg cagcggttcc gtaggcgctg ccgccgggta
    88921 ttatgcaacc cgcgccggtt taaacgtgct aatgaccgac gcccatatgc caccgcatca
    88981 acacggcagc caccacggcg atacgcgatt aattcgccat gcttatggtg aaggcgaaaa
    89041 gtatgtcccg ttggtcctcc gcgcgcaaac gctgtgggat gaactttccc gccacaacga
    89101 agatgatccc atttttgtac gctctggtgt cattaacctc ggcccggctg actccgcatt
    89161 tctcgccaac gtcgcccaca gcgccgaaca gtggcaactc aacgttgaaa agctcgatgc
    89221 gcaagggatt atggcccgct ggccagaaat acgcgtcccg gacaactaca tcggcttatt
    89281 tgagactgat tccggttttt tgcgcagcga actggcgatt aaaacctgga tccaactggc
    89341 gaaggaagcg ggctgtgcgc aactgttcaa ctgcccggtc accgcaattc gtcatgacga
    89401 tgatggcgta actattgaaa cggttgacgg tgagtatcag gcgaaaaaag cgattgtctg
    89461 cgcgggaaca tgggtaaaag acctgctccc ggagctgcct gtccagcccg tacgtaaagt
    89521 atttgcctgg tatcaggccg atggccgcta tagcgtgaag aataaattcc cggcgtttac
    89581 cggtgaactg cccaatggcg atcaatatta tggttttccg gcagaaaacg acgcgttgaa
    89641 gattggcaaa cataacggag gccaggttat ccattcagcg gatgaacgtg ttccgtttgc
    89701 ggaagtggcc agtgatgggt cagaagcctt cccgttcttg cgcaatgtat tgccgggtat
    89761 cggttgctgc ctgtacggcg ctgcctgcac ctatgataat tcgcctgacg aagattttat
    89821 tatcgatacc ctacccggcc acgataatac actgctcatt accggcctga gtgggcacgg
    89881 ttttaaattt gcgtcagttt taggggaaat agctgccgat tttgcgcaag accaaaaaag
    89941 cgattttgat ttgacgccat tcaggctttc ccgcttccaa taatcatcat tacggcctcc
    90001 agaatgaggc cgtttttaca ggagtattta atgcgtcggc tgttgcacta tctcatcaat
    90061 aatattcgcg agcatctgat gctttatctt tttctctggg gattgttggc aattatggat
    90121 ctgatctatg tgttttattt ttagttttcc gcactgacat aaactgacaa atatttaatc
    90181 tccctttcat ttagcgtgaa aaattgaatg agattattcc tgcttctaca gcactcacca
    90241 gacatctctg atgctgaacc cactaaactt atgaagattc tttcattatt aattcaaaat
    90301 tattgaaata aaaataaata tcattcaatt tgttattttt gtgttgcaat tcattctccg
    90361 acacgccatt ttctttctct cgcaaattcg tattggatgc acttatgtca ttcacaaata
    90421 cccctgaacg ctatggtgtt atatctgcgg ccttccattg gctaagcgca attattgtct
    90481 acggcatgtt tgctttaggt ctgtggatgg tcacgctcag ttattacgat ggctggtatc
    90541 acaaagcacc cgaactgcat aaaagcatcg gtattttgtt aatgatgggg ctggttattc
    90601 gcgttctgtg gcgtgtcata tctccaccgc cgggtccgct gccgagttat tcgcccatga
    90661 ctcgtcttgc cgcaaaagct ggacatctcg cattgtgcct attgctgttc gccatcggca
    90721 tcagcggcta tctgatctca actgccgatg gtaaaccgat cagcgttttt ggctggtttg
    90781 acgtccccgc gacccttgcc gacgctggcg cacaggcaga ctttgccggt gccctgcatt
    90841 tttggctcgc gtggagcgtc gtagtactgt ctgttatgca cggatttatg gccctgaagc
    90901 atcatttcat cgataaagac gacactctga agcgcatgct gggaaagtcg tcatctgact
    90961 atggagtata aaaaatgaaa aaaagcctgc ttggtttaac cttcgcgtcc ctgatgttct
    91021 ctgccggttc agcggttgcc gccgattaca aaattgacaa agaaggtcag cacgcctttg
    91081 ttaacttccg catacagcat cttggctata gctggttata tggcaccttt aaagatttcg
    91141 acggtacttt tacctttgat gaaaaaaatc cggctgccga taaagtgaat gtgacaatta
    91201 acaccaccag cgtcgatact aaccacgccg aacgcgataa acatcttcgc agtgcggatt
    91261 ttctcaatac cacaaaatat ccacaggcaa cattcacctc caccagcgtg aagaaagacg
    91321 gtgacgaact ggatattacc ggcgatctga cgctgaatgg cgtaaccaaa cccgtcacgc
    91381 tggaagcgaa attaattggt cagggcgacg acccatgggg cggtaaacgt gcaggctttg
    91441 aggccgaagg caaaattaag ctcaaagact tcaatatcaa gacagattta ggtccagctt
    91501 ctcaggaagt ggatctgatt atttcagtgg aaggcgtaca gcagaagtaa gagtgaaatg
    91561 ttgccggatg gcacgcatcc ggcaatgata tttattctgt tggatcagga atacccagtg
    91621 ttgtattcag acgtccacga gacttattaa agatcttatt gccattttca cgtcctgccc
    91681 gacggcgtcg ctgttcttct ggcggtaacg cgctttcttc gcagcaaatc tcactacaac
    91741 aacctttgta tttttccgcg catactggac actgaataaa cagcaggtgg cagccatcat
    91801 ttttacagtt ggtatggctg tcgcacggcg cgccgcactg gtggcaatgc gcgataatct
    91861 catccgatat acgttcgccc atccgctcgt caaaaacaaa atttttgcca ataaaacgca
    91921 ccggcaagcc ctgctcgcgc gccttacggg cgtattcaat aattccgccc tcgatatgcc
    91981 agactttgtt gaatccgtta tgtttcatcc aggcactggc cttttcacaa cgaatgccgc
    92041 cggtgcagta catgacgatt tttttatctt tatgtgcctg catcatctcg actgcttttg
    92101 gcagctgctc acggaaggta tctgccggaa tttcgagcgc gttttcaaag tgccccactt
    92161 catactcata gtggttacgc atgtcgataa atagtgcatc gggatcgtca agcatggcgt
    92221 tcacttccgc cgcttgcaga tactcaccaa cattgctggc atcaaagtga ggatcgtcaa
    92281 taccgtcggc aacaatgcga tcgcgtacct tcatgcgcag tacccagaag gatttcccgt
    92341 catcatccaa cgcgatattc aggcgtaaac cctctaaagc cgggtcgaag gcatagagct
    92401 gcgcgcgaaa tgtttcaaca ttgctcgcag gtacgctgat ttgcgcgtta atgccctcat
    92461 gcgccagata cactcgccca aaaacattca gcgcggtaaa cagctgatat aaagcgtcac
    92521 gggtcgcctt aggatcggcg atgtggaaat acttgtaaaa cgaaatggtg gttcgcggtt
    92581 cgctctcagc caacattttg gcttttaacg cgtcgttgga aatgcggttg tgtaacactg
    92641 gcatggtgta cggttcctgc gagatgggaa agtaaaaatc cgcggcatga tatagcaatt
    92701 atcgataatt aacatccaca cattttacgc tacatttgcg cattaaaaat tatttgttat
    92761 ttacaagcgc ggcaatttcg cccagtcttc agccacaaat tttggttgcg ggcgaaaaaa
    92821 tgcgacaata catacaattg cccgtatagg ttgaaaaaca ggattgatat gacgaatcta
    92881 cccaagttct ccaccgcact gcttcatccg cgttattggt taacctggtt gggtattggc
    92941 gtactttggt tggtcgtgca attgccctac ccgattatct accgcctcgg ttgtggatta
    93001 ggaaaactgg cgttacgttt tatgaaacga cgcgcaaaaa ttgtgcatcg caacctggaa
    93061 ctgtgcttcc cggaaatgag cgaacaagaa cgccgtaaaa tggtggtgaa gaatttcgaa
    93121 tccgttggca tgggcctgat ggaaaccggt atggcgtggt tctggccgga ccgccgaatc
    93181 gcccgctgga cggaagtgat cggcatggaa cacattcgtg acgtgcaggc gcaaaaacgc
    93241 ggcatcctgt tagttggcat ccattttctg acactggagc tgggcgcgcg gcagtttggt
    93301 atgcaggaac cggggattgg cgtttaccgc ccgaacgata atccactgat tgactggcta
    93361 caaacctggg gccgtttgcg ctcaaataaa tcgatgctcg accgcaaaga tttaaaaggc
    93421 atgattaaag ccctgaaaaa aggcgaagtg gtctggtacg caccagatca tgattacggc
    93481 ccgcgctcaa gcgttttcgt cccgttattt gccgttgagc aggctgcgac cacgaccgga
    93541 acctggatgc tggcacggat gtccggcgca tgtctggtgc ccttcgttcc acgccgtaag
    93601 ccagatggca aagggtatca attgattatg ctgccaccag agtgttctcc gccactggat
    93661 gatgccgaaa ctaccgccgc gtggatgaac aaagtggtcg aaaaatgcat catgatggca
    93721 ccagagcagt atatgtggtt acaccgtcgc tttaaaacac gcccggaagg cgttccttca
    93781 cgctattaaa tctcccatgc cggatgcttc agaatggcat ccggcattac cacagcaaat
    93841 ccccctgatt tagcgataaa agctctctgg attgcgcccc ctggaagtcg ggcgcataat
    93901 tagtgtgttt atcttttctt cttatgttca ccgcgcctgg cgcaccaaca gcggattgct
    93961 atgtcaccct gtgaaaatga cacccctata aactggaaac gaaacctgat cgtcgcctgg
    94021 ctaggctgtt ttcttaccgg ggccgccttc agtctggtaa tgcccttctt acccctctac
    94081 gttgagcagc ttggtgttac cggccactcc gccctgaata tgtggtccgg tattgtcttc
    94141 agtataatgg gttcctaaac acatcatttt aggtattgaa tatgagacgg gcaatcagct
    94201 acatcagatt ttcaagtgaa cgacaactga aaggggactc tgtacgccga caatctaagc
    94261 tggttactga ctggcttgat aagaaccctg agttttactt ggattcatcc ttgagcttta
    94321 aagatttggg gaaaagtgct tttagtggta agcacctgaa aggtggtcta ggtgactttt
    94381 taaccgctat cgagaaaggg cttgttaaag ctggtgatac gctactaatc gagagtttag
    94441 accgtctgtc acgtcaggat attgatattg cttctgagct attacgccgt atcttaaggg
    94501 ctggtgtaga tgtagtcaca ctgagcgatg gagaacatta cacacgagaa agtttaaaag
    94561 acccgttagc cctgattaaa agcattctga taatgcagcg ggcgcatgag gaaagtttga
    94621 gaaagtctga gcgtgtccaa gctgcatgga accgtaaaaa agaactcatc tccgaaggta
    94681 tcaaggtatc taggcgttgt cctgcatggt taaggctgaa tgatgaccgt cgaacattca
    94741 caatcattcc tgataaagtg gaagttgtta aacgagcatt tgacctgaga ttacagggtt
    94801 taagcttttg ggctatcacc agaactttaa acgatgaagg tcacttgtca ttgaatcaat
    94861 acacacccaa acaaaagggg tggtctgata ctgctgtaaa gaaactgctc agaaatcgtg
    94921 ctgtgattgg atgtttcaca cctgctggac gtgaagaagt tcagggttac taccccgcaa
    94981 taatcagtga gtcactattc tatcgagtac agcaactgaa cacagggcaa tatggtaggg
    95041 cttctgtatc aagtaatccg ctatcagtca acttgttcag gg
//
LOCUS       DAJTUI010000003        75634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTUI010000003 DAJTUI010000000
VERSION     DAJTUI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.003"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(435..641)
                     /locus_tag="OO464_000226"
     CDS             complement(435..641)
                     /locus_tag="OO464_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6225519.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="OO464_000227"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="OO464_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6225520.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="OO464_000228"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="OO464_000228"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR6225521.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4786
                     /gene="kdpB"
                     /locus_tag="OO464_000229"
                     /pseudo
     CDS             2739..4786
                     /gene="kdpB"
                     /locus_tag="OO464_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="OO464_000230"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="OO464_000230"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR6225522.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="OO464_000231"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="OO464_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8722
                     /gene="kdpE"
                     /locus_tag="OO464_000232"
                     /pseudo
     CDS             8037..8722
                     /gene="kdpE"
                     /locus_tag="OO464_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8997..9101
                     /locus_tag="OO464_000233"
     CDS             8997..9101
                     /locus_tag="OO464_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR6225523.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9412..11609
                     /gene="speF"
                     /locus_tag="OO464_000234"
                     /pseudo
     CDS             9412..11609
                     /gene="speF"
                     /locus_tag="OO464_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11606..12925
                     /gene="potE"
                     /locus_tag="OO464_000235"
     CDS             11606..12925
                     /gene="potE"
                     /locus_tag="OO464_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6225524.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12981..14621)
                     /gene="pgm"
                     /locus_tag="OO464_000236"
     CDS             complement(12981..14621)
                     /gene="pgm"
                     /locus_tag="OO464_000236"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6225525.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14647..15192)
                     /gene="seqA"
                     /locus_tag="OO464_000237"
     CDS             complement(14647..15192)
                     /gene="seqA"
                     /locus_tag="OO464_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6225526.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15377..16141
                     /gene="ybfF"
                     /locus_tag="OO464_000238"
     CDS             15377..16141
                     /gene="ybfF"
                     /locus_tag="OO464_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6225527.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16281..16574
                     /gene="ybfE"
                     /locus_tag="OO464_000239"
     CDS             16281..16574
                     /gene="ybfE"
                     /locus_tag="OO464_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6225528.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16714..17244
                     /gene="fldA"
                     /locus_tag="OO464_000240"
     CDS             16714..17244
                     /gene="fldA"
                     /locus_tag="OO464_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6225529.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17454..17540
                     /gene="uof"
                     /locus_tag="OO464_000241"
     CDS             17454..17540
                     /gene="uof"
                     /locus_tag="OO464_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6225530.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17533..17979
                     /gene="fur"
                     /locus_tag="OO464_000242"
     CDS             17533..17979
                     /gene="fur"
                     /locus_tag="OO464_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6225531.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18063..18389)
                     /locus_tag="OO464_000243"
     CDS             complement(18063..18389)
                     /locus_tag="OO464_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225532.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18426..19832)
                     /gene="chiP"
                     /locus_tag="OO464_000244"
     CDS             complement(18426..19832)
                     /gene="chiP"
                     /locus_tag="OO464_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR6225533.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20411..22075)
                     /gene="glnS"
                     /locus_tag="OO464_000245"
     CDS             complement(20411..22075)
                     /gene="glnS"
                     /locus_tag="OO464_000245"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6225534.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22278..24224)
                     /gene="nagE"
                     /locus_tag="OO464_000246"
     CDS             complement(22278..24224)
                     /gene="nagE"
                     /locus_tag="OO464_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6225535.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24557..25357
                     /gene="nagB"
                     /locus_tag="OO464_000247"
     CDS             24557..25357
                     /gene="nagB"
                     /locus_tag="OO464_000247"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6225536.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25417..26565
                     /gene="nagA"
                     /locus_tag="OO464_000248"
     CDS             25417..26565
                     /gene="nagA"
                     /locus_tag="OO464_000248"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6225537.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26574..27794
                     /gene="nagC"
                     /locus_tag="OO464_000249"
                     /gene_synonym="nagR"
     CDS             26574..27794
                     /gene="nagC"
                     /locus_tag="OO464_000249"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6225538.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27842..28594
                     /gene="nagD"
                     /locus_tag="OO464_000250"
                     /pseudo
     CDS             27842..28594
                     /gene="nagD"
                     /locus_tag="OO464_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            28991..30655
                     /gene="asnB"
                     /locus_tag="OO464_000251"
     CDS             28991..30655
                     /gene="asnB"
                     /locus_tag="OO464_000251"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6225539.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31034..31110
                     /locus_tag="OO464_000252"
     tRNA            31034..31110
                     /locus_tag="OO464_000252"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31068..31070,aa:Met,seq:cat)
     gene            31119..31203
                     /locus_tag="OO464_000253"
     tRNA            31119..31203
                     /locus_tag="OO464_000253"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31153..31155,aa:Leu,seq:tag)
     gene            31227..31301
                     /locus_tag="OO464_000254"
     tRNA            31227..31301
                     /locus_tag="OO464_000254"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31259..31261,aa:Gln,seq:ttg)
     gene            31336..31410
                     /locus_tag="OO464_000255"
     tRNA            31336..31410
                     /locus_tag="OO464_000255"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31368..31370,aa:Gln,seq:ttg)
     gene            31426..31502
                     /locus_tag="OO464_000256"
     tRNA            31426..31502
                     /locus_tag="OO464_000256"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31460..31462,aa:Met,seq:cat)
     gene            31551..31625
                     /locus_tag="OO464_000257"
     tRNA            31551..31625
                     /locus_tag="OO464_000257"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31583..31585,aa:Gln,seq:ctg)
     gene            31663..31737
                     /locus_tag="OO464_000258"
     tRNA            31663..31737
                     /locus_tag="OO464_000258"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31695..31697,aa:Gln,seq:ctg)
     gene            complement(31891..33066)
                     /gene="ubiF"
                     /locus_tag="OO464_000259"
     CDS             complement(31891..33066)
                     /gene="ubiF"
                     /locus_tag="OO464_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6225540.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33212..34636
                     /gene="miaB"
                     /locus_tag="OO464_000260"
     CDS             33212..34636
                     /gene="miaB"
                     /locus_tag="OO464_000260"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6225541.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34789..35829
                     /locus_tag="OO464_000261"
     CDS             34789..35829
                     /locus_tag="OO464_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6225542.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35826..36293
                     /gene="ybeY"
                     /locus_tag="OO464_000262"
     CDS             35826..36293
                     /gene="ybeY"
                     /locus_tag="OO464_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6225543.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36383..37261
                     /gene="corC"
                     /locus_tag="OO464_000263"
     CDS             36383..37261
                     /gene="corC"
                     /locus_tag="OO464_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6225544.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37286..38824
                     /gene="lnt"
                     /locus_tag="OO464_000264"
     CDS             37286..38824
                     /gene="lnt"
                     /locus_tag="OO464_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6225545.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39221..40129
                     /gene="gltI"
                     /locus_tag="OO464_000265"
     CDS             39221..40129
                     /gene="gltI"
                     /locus_tag="OO464_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR6225546.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40299..41039
                     /gene="gltJ"
                     /locus_tag="OO464_000266"
                     /pseudo
     CDS             40299..41039
                     /gene="gltJ"
                     /locus_tag="OO464_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
     gene            41039..41713
                     /gene="gltK"
                     /locus_tag="OO464_000267"
     CDS             41039..41713
                     /gene="gltK"
                     /locus_tag="OO464_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6225547.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41713..42438
                     /gene="gltL"
                     /locus_tag="OO464_000268"
     CDS             41713..42438
                     /gene="gltL"
                     /locus_tag="OO464_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR6225548.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42556..43491
                     /gene="rihA"
                     /locus_tag="OO464_000269"
     CDS             42556..43491
                     /gene="rihA"
                     /locus_tag="OO464_000269"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6225549.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43575..45245
                     /gene="hscC"
                     /locus_tag="OO464_000270"
                     /pseudo
     CDS             43575..45245
                     /gene="hscC"
                     /locus_tag="OO464_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45305..46756)
                     /gene="djlC"
                     /locus_tag="OO464_000271"
     CDS             complement(45305..46756)
                     /gene="djlC"
                     /locus_tag="OO464_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR6225550.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46753..47460)
                     /locus_tag="OO464_000272"
     CDS             complement(46753..47460)
                     /locus_tag="OO464_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6225551.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47562..48116
                     /locus_tag="OO464_000273"
     CDS             47562..48116
                     /locus_tag="OO464_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6225552.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48126..49553)
                     /locus_tag="OO464_000274"
     CDS             complement(48126..49553)
                     /locus_tag="OO464_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6225553.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49550..50257)
                     /locus_tag="OO464_000275"
     CDS             complement(49550..50257)
                     /locus_tag="OO464_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6225554.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50421..51398
                     /locus_tag="OO464_000276"
     CDS             50421..51398
                     /locus_tag="OO464_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6225555.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51468..51950)
                     /locus_tag="OO464_000277"
     CDS             complement(51468..51950)
                     /locus_tag="OO464_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6225556.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52185..54767
                     /gene="leuS"
                     /locus_tag="OO464_000278"
     CDS             52185..54767
                     /gene="leuS"
                     /locus_tag="OO464_000278"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6225557.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54782..55363
                     /gene="lptE"
                     /locus_tag="OO464_000279"
     CDS             54782..55363
                     /gene="lptE"
                     /locus_tag="OO464_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6225558.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55363..56394
                     /gene="holA"
                     /locus_tag="OO464_000280"
     CDS             55363..56394
                     /gene="holA"
                     /locus_tag="OO464_000280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6225559.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56396..57037
                     /gene="nadD"
                     /locus_tag="OO464_000281"
     CDS             56396..57037
                     /gene="nadD"
                     /locus_tag="OO464_000281"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6225560.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            57061..57672
                     /locus_tag="OO464_000282"
     CDS             57061..57672
                     /locus_tag="OO464_000282"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR6225561.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57932..58249
                     /gene="rsfS"
                     /locus_tag="OO464_000283"
                     /gene_synonym="rsfA"
     CDS             57932..58249
                     /gene="rsfS"
                     /locus_tag="OO464_000283"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6225562.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58253..58720
                     /gene="rlmH"
                     /locus_tag="OO464_000284"
     CDS             58253..58720
                     /gene="rlmH"
                     /locus_tag="OO464_000284"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6225563.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58751..60652
                     /gene="mrdA"
                     /locus_tag="OO464_000285"
     CDS             58751..60652
                     /gene="mrdA"
                     /locus_tag="OO464_000285"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6225564.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60655..61767
                     /gene="mrdB"
                     /locus_tag="OO464_000286"
                     /gene_synonym="rodA"
     CDS             60655..61767
                     /gene="mrdB"
                     /locus_tag="OO464_000286"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6225565.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61778..62866
                     /gene="rlpA"
                     /locus_tag="OO464_000287"
     CDS             61778..62866
                     /gene="rlpA"
                     /locus_tag="OO464_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6225566.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            63005..64216
                     /gene="dacA"
                     /locus_tag="OO464_000288"
     CDS             63005..64216
                     /gene="dacA"
                     /locus_tag="OO464_000288"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6225567.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64327..64590
                     /locus_tag="OO464_000289"
     CDS             64327..64590
                     /locus_tag="OO464_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6225568.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64691..65332
                     /gene="lipB"
                     /locus_tag="OO464_000290"
     CDS             64691..65332
                     /gene="lipB"
                     /locus_tag="OO464_000290"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6225569.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65591..66544
                     /locus_tag="OO464_000291"
     CDS             65591..66544
                     /locus_tag="OO464_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6225570.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66753..67718
                     /gene="lipA"
                     /locus_tag="OO464_000292"
     CDS             66753..67718
                     /gene="lipA"
                     /locus_tag="OO464_000292"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6225571.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67819..68022)
                     /gene="tatE"
                     /locus_tag="OO464_000293"
     CDS             complement(67819..68022)
                     /gene="tatE"
                     /locus_tag="OO464_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR6225572.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68151..68939)
                     /locus_tag="OO464_000294"
     CDS             complement(68151..68939)
                     /locus_tag="OO464_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR6225573.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            69032..69415
                     /gene="crcB"
                     /locus_tag="OO464_000295"
     CDS             69032..69415
                     /gene="crcB"
                     /locus_tag="OO464_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6225574.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69469..69678)
                     /gene="cspE"
                     /locus_tag="OO464_000296"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69469..69678)
                     /gene="cspE"
                     /locus_tag="OO464_000296"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6225575.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69853..70413)
                     /gene="pagP"
                     /locus_tag="OO464_000297"
     CDS             complement(69853..70413)
                     /gene="pagP"
                     /locus_tag="OO464_000297"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6225576.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            71002..72387
                     /gene="dcuC"
                     /locus_tag="OO464_000298"
     CDS             71002..72387
                     /gene="dcuC"
                     /locus_tag="OO464_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6225577.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(72428..72769)
                     /locus_tag="OO464_000299"
                     /pseudo
     CDS             complement(72428..72769)
                     /locus_tag="OO464_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73107..73616)
                     /locus_tag="OO464_000300"
     CDS             complement(73107..73616)
                     /locus_tag="OO464_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6225578.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73779..74024
                     /locus_tag="OO464_000301"
     CDS             73779..74024
                     /locus_tag="OO464_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6225579.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            73999..75630
                     /locus_tag="OO464_000302"
                     /pseudo
     CDS             73999..75630
                     /locus_tag="OO464_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        18614 a        18994 c        19211 g        18815 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggctatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgttaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgagg catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggaattacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ctccgccacc gccgcgcccg atccgctggt gaaattttcc ggtgttaccg tcgatttagc
     8461 cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acaccaattg agttccgcct
     8521 gctggcggtg ctgctcaaca atgccggaaa agtactcacc cagcgccagc tccttaacca
     8581 ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct
     8641 gcgacaaaaa ctggaacagg atccggcccg gcctcgccac ttcattactg aaaccggtat
     8701 tgggtatcgg tttatgcttt gaattgttat tcaataaatt cgcttaaata gtgattaatt
     8761 tattattaaa cctgcgccga atattattat ttggcaggtt taatttctta acgcaaatat
     8821 aaaacacgaa attacaacat ttaagtaaca aaacagcatc taatatggcg atattcataa
     8881 ttaatgattt attatctggg gtatgatcgt tttttgttga tcttcttcac agattatagc
     8941 catttcatgg atagaataac tctaccttca actgacacag caagcggtaa aggtaaatgg
     9001 aaaataataa ccgcttaatg cctcatataa ggcggacaac ccatatcatg aagtttgccc
     9061 atcgtaatag ttttgacttt catttcttta atgcccgcta gtcttctgac taaagggcac
     9121 cccaacgtac aggtctccct gactttaagc attacaggtt aatacctgta ttcctcggtg
     9181 ctcatatact gctaaccctt ttaaactcta aataattcga gtcgcagcac ttgcaacttg
     9241 aggtatgacg agtatagcca gttaccgggc tggtctgggt tattgcatct gcaaaaagca
     9301 aactactgat ttatttatca gcggtggagc tttgcttttt ttccgacgtg atcgatttct
     9361 cctttgagaa attgaggacc tgctattacc tgaaataaag agatgaataa aatgtcagaa
     9421 ttaaaaattg ccgttagccg ttcttgcccg gattgttttt ccactcatcg tgcatgcgtg
     9481 aatattgacg aaagtaatta tattgacgtt gccgccatta ttttatcagt caatgatgtt
     9541 gaacgtggaa aactcgataa aatagacgct actggctatg gcattcctgt ttttattgca
     9601 acggaaaatg aagaacgtgt accagcagaa tatctgccac gtatttctgg tgtcttcgag
     9661 cattgcgaat cacggaaaga gttttatgga cgtcagttag aaaccgctgc tagccattat
     9721 gaaacgcaac tgcgcccgcc cttcttccgc gcactggtcg attatgtcaa tcagggcaat
     9781 agcgcttttg actgcccggg tcatcaggga ggtgagtttt tccgtcgtca tccggcaggg
     9841 aatcagttcg tggagtactt cggagaaacg ctgttccgtt ccgacttatg caacgcagac
     9901 gtagcaatgg gcgatctgct gattcacgaa ggcgcgccat gcattgcaca gcaacatgcg
     9961 gcaaaagtgt ttaatgccga taaaacatac ttcgttctaa atggcacttc atcttctaac
    10021 aaagtggttt taaacgccct gctaacaccg ggtgatctgg tgctgtttga tcgcaataac
    10081 cacaaatcta accaccacgg agcgttgcta caggctggtg caacaccggt ttatctggaa
    10141 acggcacgta acccgtatgg ctttatcggt ggcattgatg cgcactgttt tgaagaaagt
    10201 tacctgcgtg agctgatcgc ggaagtcgca ccgcagcggg caaaagaggc tcgtcctttc
    10261 cgcctcgctg tgattcagtt aggcacctac gacggtacga tttataacgc ccgccaggtg
    10321 gtggataaaa ttggtcatct gtgtgactac attctgtttg actccgcatg ggttggctat
    10381 gagcaattta tcccgatgat ggctgactgt tcgccgctgt tgctggatct taatgagaac
    10441 gatccgggta ttctagttac gcaatccgta cataaacagc aggctggttt ttctcaaact
    10501 tcacaaatcc ataaaaaaga cagccacatc aaagggcaac agcgttatgt accgcacaaa
    10561 cgcatgaaca acgcctttat gatgcacgcc tccaccagcc cgttctatcc gctgtttgcc
    10621 gcactggata tcaacgccaa aatgcatgaa ggtgtcagcg gtcgtaatat gtggatggat
    10681 tgtgtggtaa atggcattaa tgcccgcaaa ctgatcctcg ataactgtca gcatattcgt
    10741 ccgttcgtac ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac agcgcaaatt
    10801 gcggttgatc tgcgcttctt ccagtttgta ccaggggaac actggcattc ttttgaaggc
    10861 tatgcagaga atcaatactt tgtcgatcct tgcaaactgt tgctgacaac cccaggtatt
    10921 gatgcacgta acggcgaata tgaagcgttc ggtgtacccg cgacgattct tgctaacttc
    10981 ctgcgcgaaa atggcgtagt gccggaaaaa tgcgatctta acgccatcct cttcctgctg
    11041 actccggcag aagatatggc caaacttcag caacttgtgc cctgctggta cgcttcgaaa
    11101 aactgcttga gtccgacgcg ccattagcag aagtgctgcc ttccatctac aaacagcatg
    11161 aagagcgcta cgccggttat accctgcgtc agttgtgcca ggaaatgcat gatttgtatg
    11221 cccgccacaa cgtgaaacaa ctgcaaaaag agatgttccg caaagcgcac ttcccacacg
    11281 tcagcatgaa tccgcaagaa gccaactacg cctatttacg cggtgaagtg gagctggttc
    11341 gtctaccgga tgccgaaggt cgtatcgctg ccgaaggggc gcttccttat cctccgggtg
    11401 tgctgtgtgt tgttccgggt gaaatctggg gtggtgctgt tttgcgttac ttcagcgctc
    11461 tggaagaagg gatcaacctg ctgccaggtt ttgcaccgga gctgcagggt gtctatatcg
    11521 aagaacatga tggtcgtaag caagtttggt gctatgtcat caagcctcgt gatgcgcaaa
    11581 gcaccctgtt gaaaggggaa aaattatgag tcaggctaaa tcgaacaaaa tgggcgtcgt
    11641 tcagttaacc atactgacga tggtcaacat gatgggctcc ggtatcatca tgctgccgac
    11701 aaagcttgcc gaagttggga caatttcaat tatctcctgg ctggtgacag ccgtaggctc
    11761 aatggcactg gcatgggcat tcgcaaaatg cggtatgttc agccgtaaat caggtggtat
    11821 gggcggttat gccgagtatg ctttcggtaa atccggtaac tttattgcga actataccta
    11881 cggcgtctca ctgctgattg ctaacgtcgc gattgctatt tcggcggttg gttacggcac
    11941 cgaactgctc ggcgcgagtt tgtcgccagt gcagattggt cttgcgacca tcggggtgct
    12001 gtggatttgt accgtggcta actttggtgg tgcgcgcatt accgggcaaa tcagtagcat
    12061 taccgtgtgg gggggcatta ttccggtggt aggcctgtgc attatcggct ggttctggtt
    12121 tagcccgacg ctttacgttg attcctggaa tccgcatcat gcaccgttct tcagtgcagt
    12181 aggttcttcc atcgctatga cgctgtgggc ttttcttggt ctggagtctg cgtgtgcgaa
    12241 tactgatgta gtggaaaacc cggaacgtaa tgtgccaatc gcggtactcg gcggtacgtt
    12301 aggtgcggcg gtgatttata tcgtctccac caacgtgatt gcagggattg tgcctaatat
    12361 ggagctggca aattcaacgg caccatttgg cctggccttc gcacagatgt ttacaccaga
    12421 agtgggtaaa gtcattatgg cgctgatggt gatgtcctgc tgcggttcgc tgcttggctg
    12481 gcagttcacc attgcccagg tgtttaaatc ttcatctgat gaaggctact tccctaaaat
    12541 tttctcccgt gtaaccaaag tggatgcacc ggtgcaggga atgttgacca ttgtgattat
    12601 ccagagcggg ttggcgctga tgacgattag cccgtcgcta aacagtcagt tcaacgtgct
    12661 ggttaacctg gccgtggtga ccaatatcat tccgtatatt ctgtcaatgg cggcattagt
    12721 cattattcag aaggtcgcta atgtgccccc ctcaaaagcg aaagttgcaa actttgttgc
    12781 ttttgttggc gcgatgtata gcttttatgc gctgtactca tccggggaag aagccatgct
    12841 gtacggttcc atcgtgacct tccttggttg gacactgtat ggtctggtat caccacgctt
    12901 tgaactgaaa aataaacacg gttaaaaaag caaagtaaaa aagggcgatc ttgcgaccgc
    12961 tcttttttta ttaaatgtgt ttacgcgttt ttcagaactt cgctaacaat ctcaaccgct
    13021 tctttctcaa tctgcttgcg atgttcttca ccgaggaagc tttcgcagta gatcttatat
    13081 gcgtcttccg tgcctgacgg acgcgcggcg aaccagccgt tgtcagtcat cactttcaga
    13141 ccgccaatag aagcaccgtt gcccggagca gcagtcaggc gcgcggtgat cgggtcacct
    13201 gccagggtgc tggcgctcac catttccgga gacagcttag acagcgccgc tttttgtgcg
    13261 gaagtcgcag ctgcctgcaa acggttgtag ctcggcgcac caaagcgttt tgccagttcg
    13321 ttgtagtgtt cctgcgggtt cttaccggtg acagcggtga tttccgccgc cagcagacac
    13381 ataatgatgc cgtctttgtc ggtggaccac ggcgtgccgt cgaaacgcag gaaggaagcc
    13441 cctgcgctct cttcgccgcc aaagccgaag ctgccgtcga acagaccatc aacaaaccat
    13501 ttgaaaccta ccggtacttc taccagctta cggcccaagt cattgaccac acggtcgatc
    13561 atcgcagaag aaaccagcgt tttaccgaca gcaacatctt tgccccactg cggacgatgc
    13621 tggaacaggt agttgatcgc caccgccagg tagtggttcg gattcatcaa acctgccgga
    13681 gtgacgatac cgtgacggtc ataatccggg tcgttagcaa acgccagatc gaacttatca
    13741 cgcagtgcca gcagacccgc catcgcacac tcggaggagc agtccatacg gatcgcaccg
    13801 tctttatcga ggtgcataaa gcggaaggtt tgatcgacct gatcgttaac gatggtcagg
    13861 ttgaggttgt aatactcgcc aatgcgcttc cagtattcga taccggaacc gcccaacgga
    13921 tcaacgccca gcgtcaggcc cgctttctga atcgccgcca tatcaacgat atcggccagc
    13981 ccttccacga acggctgcac cagatcctgc tctttcacat agccggatgc catcgcttcg
    14041 tcgagggaga tacgcttcac gcctttcagg ccatcggcca tcagtgcgtt ggccttgtct
    14101 tccaccactt tggtgacgtt agtatcagcc gggccaccat ttggcggatt gtacttgata
    14161 ccaccatctt ccggcgggtt atgggacggt gtaatcacga taccgtctgc cagcgggcca
    14221 ccttttttat tgtgaaccag gatggcattg gaaacggcag gcgtcggagt gaagccattg
    14281 ttttcctgca caatgacatc aacgccgttc gctgccagca cttccagtac ggaaatgaac
    14341 gcaggctcgg acagggcgtg agtatcttta cccacatagc aagggccagt gatgccgttt
    14401 ttcgcacgtt cttcagcaat tgcctgagcg atcgccagaa tgtgcggctc gttaaagctg
    14461 tggcgcgctg cactgccacg gtgaccggaa gtaccgaatt tcaccgcgtg ctccgcattc
    14521 cctgcttctg gtttcagtac ataatattgc gccgtcagtt gggcgacgtt aatcaaatca
    14581 ctctgttgtg caggttggcc tgcacgattg tggattgcca ttgctttgtc ctttgtctgc
    14641 aacgttttag atagttccgc aaaccttctc aatcaattcc gccgggaatt gcatcgactg
    14701 catgatgtgt tcgatcatgc tgcatttacg gccggtgttg gtgttggtga tcacccaata
    14761 cggcgtgcct ggcacatgtt tcggcttggt ctgattacca tttttcagca gcgtttgttc
    14821 atctgccgca aagtacacgc gtgtacgacc gtgcaacgat tccgttgctt cggcaaacgc
    14881 ctgggcgtca agagaatata gtgtagacaa cagcagcata aagcgattga ccgctcgctt
    14941 ttgctctgcg tattcgtccg aaagcagaag ttcacgcatt gcgcgaacct tgtctttaat
    15001 cgttttgacc ggcttcgctt cgacgatagc aggtgacgca acgcgaacct ctttcgtcac
    15061 cggagcagca ggctgtgatg cggcggaaaa tttcaacata cgccgtaaaa tgtcggatgc
    15121 gctctcgccg atatgcttag tgtggctggc aatatagctg tagagttcat catcaacttc
    15181 aatcgttttc atcttaatcc agtgcagtgt cttagctgaa tacaagtcgc caagagtata
    15241 tgaataaatc ccggcagcgg atagcgtcaa gcctggcttg cagaaaaact cggaataaac
    15301 cttatttgtt gcagccctgt ggcacaatgg tcaacataat accctaaccc gacaacgcga
    15361 aaaaaagaac tttgccatga aattaaatat ccgcgcgcaa actgcacaaa accagcacaa
    15421 taattctccc atcgttcttg tccatggtct gtttggtagc ctcgacaacc ttggcgtact
    15481 ggctcgcgat ctggtaaacg atcacaatat catccaggtt gatatgcgta accacggtct
    15541 ttcaccgaga gagccggtaa tgaattaccc ggcgatggcg caggatcttg ttgataccct
    15601 ggatgcactg cagatcgaca aagcaacatt tatcggtcac tccatgggcg gtaaagcggt
    15661 aatggcactt actgcactgg cccccgatcg catcgataaa ctggtggcga tcgatatcgc
    15721 accggtcgac tatcacgtac gccgtcatga tgagattttc gcagctatca acgcggtcag
    15781 tgaatcggac gcacaaactc gccagcaagc agcagcaata atgcgccagc atcttaatga
    15841 agaaggggtg attcagtttc tgttgaaatc ttttgttgac ggggagtggc gctttaacgt
    15901 gccggtattg tgggatcagt atccgcatat tgtgggttgg gagaaaatcc cggcatggga
    15961 tcaccctgcc ctgtttatcc ctggcggcaa ttctccgtat gttagcgagc agtaccgtga
    16021 tgatttactg gcacaatttc cacaggcacg ggcgcatgtg attgcaggcg cgggtcactg
    16081 ggtccatgct gaaaaaccgg atgcggtatt acgcgctatc cgtcgctatc tcaatgatta
    16141 acgcaactga ttaaaaaccc agcgtccgcg tcgctttccg cgcgtgggcg ttggcgtgac
    16201 tcatcgcgct gatgtattat ggcgcgctat ccatccgggc agaggcctgg ctgattgttt
    16261 cccccgaagt caccaagatc atggccaaag aacaaacgga ccgtacgaca ttagatctgt
    16321 tcgcgcacga gcgtcgaccg ggacgaccga aaactaatcc gctttcgcgc gatgaacagc
    16381 tgcgtattaa taaacgcaac cagctaaaac gcgacaaagt acgtggcctt aagcgtgtcg
    16441 aactgaagct gaacgcggaa gctgtcgagg cgctgaacga gctggcggaa tcgcgtaata
    16501 tgagccgtag cgaactgatc gaagagatgt taatgcagca actggcggcg ctgcgtagtc
    16561 agggcatcgt ttaaatttcc actttcatgt agcacagtgt gcagtcctgc tcgtttgctg
    16621 attccgcaag actgcctgtt ctgctatgat tgcctttatc cgtgggcaat tttccacccc
    16681 catttcaata agtttcaaga ggttatttca ctcatggcta tcactggcat ctttttcggc
    16741 agcgacaccg gtaataccga aaatatcgca aaaatgattc aaaaacagct tggtaaagac
    16801 gttgccgatg tccatgacat tgcaaaaagc agcaaagaag atctggaagc ttacgacatt
    16861 ctgctgctgg gcatcccaac ctggtattac ggcgaagcgc agtgtgactg ggatgacttc
    16921 ttcccgactc tcgaagagat tgatttcaat ggtaaactgg ttgcgctgtt tggttgtggt
    16981 gaccaggaag attacgccga atatttctgc gacgcattgg gcaccatccg cgacatcatt
    17041 gaaccgcgcg gtgcaaccat cgttggtcac tggccaactg cgggctatca tttcgaagca
    17101 tcaaaaggtc tggcagatga cgaccacttt gtcggtctgg ctatcgacga agaccgtcag
    17161 ccggaactga ccgctgaacg tgtagaaaaa tgggttaaac agatttctga agagttgcat
    17221 ctcgacgaaa ttcttaatgc ctgatgtgat gcggcgtaga ctcatgtcta cgccgtatta
    17281 atagataatg ccaatcaaaa taattgctac aaatttgtaa cttttgctgt tgtacctata
    17341 caatgtccca gtgttcaagt ggccttgccg ttgtaaatgt aagctgtgcc acgtttttat
    17401 taacaatatt tgccagggac ttgtggtttt catttaggcg tggcaattct ataatgatac
    17461 gcattatctc aagagcaaat tctgtcactt cttctaatga agtgaaccgc ttagtaacag
    17521 gacagattcc gcatgactga taacaatacc gccctaaaga aagctggcct gaaagtaacg
    17581 cttcctcgtt taaaaatcct ggaagttctt caggagccgg acaaccatca cgtcagtgcg
    17641 gaagatttat acaaacgtct gatcgatatg ggtgaagaaa ttggtctggc tacggtatat
    17701 cgcgtactga accagtttga cgacgctggt atcgtcaccc gccacaattt tgaaggcggt
    17761 aaatccgtat ttgaactgac acagcaacat caccacgatc acctgatttg cctcgactgc
    17821 ggcaaggtta tcgaatttag tgatgattcc atcgaagcgc gtcagcgtga aattgccgca
    17881 aaacatggca ttcgcctgac taaccacagt ctctatcttt acggtcactg tgccgaaggc
    17941 gattgccgcg aagatgagca cgcgcacgaa ggcaaataag ccagcctgaa cgagaaaagc
    18001 caacctgcgg gttggctttt ttatacaagg gaaataagaa aagcgagagt tacagctctc
    18061 acttatttgt tattactgcg tatttcctgc cagaccttat cacaatcggc tttcaccgcc
    18121 tgatcatttc cggtttgcgt tgcacgcaga cactgctgat aatccagtac gcgtacactt
    18181 tcctggtcgg caaactgctg atgttgtttc tctttcttca gcacgtttaa cacgctctgg
    18241 caggcttcaa ttttttctgg cgaaccttgc gcggtgttga tacaggcgct gtatgcctct
    18301 ttcagacggc tgtcttcctg tggtgcggtt gattgcgcac aagctaccag ccccgatgcc
    18361 atcatggcga tgagaatcag ttttttcatt gaatgcggca ggcacaaaac ctgccgcgtc
    18421 gggcatcaga agatggtgaa tggtgcgatt accataaatt tcacgtcacg ctcatcctgg
    18481 aagatgttac cgtaaccgcc gccccagctt gggatgtcgg agtggttgtc gtattcggtg
    18541 aagtgcagtt tgaacatcgt gcctttggcg cgaccgtcct gaatggtgta gaccgcatcc
    18601 aggctgtagg cagactcttc aatagtccgg tttttgtcgt agtacgcatc cggattgctc
    18661 tgccaggtcg caggtttagc atcccatgcg taaacgtagg aagcgccgat ggcgaagcct
    18721 ggaagattcc agtttttcag gtcatacatc gcactgaaga agaccgcttt ttcgccgtta
    18781 gcgttgaagt cggaacggtt atcccaccag atatccaggc ggccgttgga ggaagcgtag
    18841 gttggggtca tacgttgcag gaagtatccc tgctgactgt cagccttaat ccaggtgcct
    18901 tcgaggcgca aatccactac gtcagccgcc cggtaaccaa aggtcaacgc ttgcagccag
    18961 gcggtgccgt cataaagatc gttgacgctg cgatcgtcaa ctttatcgcg ggtaccatag
    19021 aactggtagc tggtggttaa cgggctaccg gcgatatcaa atttgtagct ggctttggca
    19081 aaatattgat cgatataccc ttccgcctga ccaaatgccg cttccagtac gaagttattt
    19141 ttgaagtcgt atttcgcacc gatggagtgc agataatcaa ctttggtggt tttatcgttc
    19201 tgataaaact catccatttc cagatgccac ggcgctttgt attcgttggt ccacatgtag
    19261 gagaaactca aggcaccagc atcgccgtaa tcaaaattcg ccccggcttc cgccccctga
    19321 taagtacccg gcataaagct ccagtgcggc gctaacagcg tttgaccggt tggctgaatg
    19381 taacctgccc tcgcccaaac cggaccgtat ttaaatttgg ccgcagcttt atacaggctt
    19441 ataccgcttt tgtcgccgga ccagtcttcg tcataggctt tattactttt tgaaaacgcg
    19501 atttcgttcg ggtgggagct gtcgccgttt tccgccattt caatcgccgt aaacgcagca
    19561 atatcaaggc cgaacatatc agcagcataa ccggactgaa aatcgaggtt ggcattccag
    19621 gtggagtgag aaaggttggt tttgtatttg tcgccgtcgg taacatcttt acggtcgcgt
    19681 tcacgctgcc agtaatagat accgccggtt aaggttgaat cgtcgatgaa tccttcggcg
    19741 cgagcctccg gcattgccat aaagcccgac attgctgtaa caccggcaat agccagcgcc
    19801 agcgtactac gtttgccact aaacgtacgc atgggttaat cctctttgac gtataaattg
    19861 ctgcaccaaa ggtgaacagc gaaaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt
    19921 ctcgctgact acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa
    19981 ccagaatgta agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa
    20041 gtttgcggtt gaaaggcttg cagaagaata aaaacgtgaa attccagcaa gatgaaagcg
    20101 gttacatttt atttaattat gttttgtgat tttaaattta ttttatggat aaattgcgaa
    20161 gcggaattaa ttcgcgaaga aaagcagaaa aaacgccgca gagcgcggcg tttgggattg
    20221 tttgctattt cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcacgcaaa
    20281 ttcaatatat tgcagagatc atgtaggcct gataagcgaa gcgcatcagg caatttagcg
    20341 tttgaaattt attgtctatt tattgccgga tgcgacgtaa atgcctcacc cggcttggta
    20401 gaacttaaaa ttactcgcct actttcgccc aggtatcacg cagcccaacg gtgcggttaa
    20461 ataccggttt ttcggccgtg gaatggcggc tgtcgaggca gaagtaacct tcacgctcaa
    20521 actggaatgc tttacccgca accgcatctt tcagcgacgg ttcagcaaag ccctgtttga
    20581 tcaccagcga ttccgggtta atcaccgaca ggaaatcatc cgcagcacct gggttaggca
    20641 cgctgaacag acgatcatac aaacggattt caaccggcag cgcatgtgcc gcgctcaccc
    20701 agtgaataac acctttgact ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt
    20761 cataagtaca gaagatggtg gtgatattac cttcggcatc tttctccacg cgttcagcct
    20821 taatgacgta agcattacgc agacgcactt ctttacccag caccagacgt ttgtactgct
    20881 tgttagcttc ttcgcggaaa tcggcgcgat cgatccaaat ttcaccgcta aacggcacct
    20941 gacggctccc catttccggt ttgttcggat ggttcggcat ggtgaccatt tcgccttcgc
    21001 cctgatagtt ttcgataacc agtttcaccg gatcgataac cgccattgcg cgcggcgcat
    21061 tttcgttgag atcttcacgg atgcaggatt ccagcgacgc catctcaatg gtgttgtcct
    21121 gcttggtcac gccgatgcgt ttgcagaact cacgaataga agccgcagtg taaccacgac
    21181 gacgcagacc ggaaatggtc ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg
    21241 tcaccagcag gttcagctta cgcttggaca tcacagtgta ttccagattc aggcgcgaga
    21301 attcgtactg gcgcggatga acaggaatgg tgatgttatc cagtacccag tcgtacagac
    21361 gacggttgtc ctggaactca agcgtacaca gagagtgcgt aataccttcc agcgcatcgc
    21421 tgatgcaatg ggtgaagtcg tacatcgggt agatgcacca cttgttgcca gtctggtggt
    21481 gttcagcaaa tttaatgcgg tacagcaccg gatcgcgcat tacgataaat ggcgacgcca
    21541 tgtcgatttt cgcacgcagg caggctttac cttcttcaaa accaccggca cgcatttttt
    21601 cgaacagcgc caagttctct tcaacgctgc ggtcgcggta cgggctgttt ttacccggct
    21661 gcgtcagggt accgcggtat tcgcggatct gttccggtgt cagttcatca acgtacgcca
    21721 ggcctttatt gatcagttcg atcgcatagg cgtggagctg atcaaaataa tcggaggagt
    21781 aacggacgtt accagaccag tgaaaaccta accactctac gtcgtttttg atcgaatcga
    21841 catactcgat atcttctttt accgggttag tgtcgtcgaa acgcaggttg cactggcctt
    21901 tatagtcctg ggcgatcccg aagttcaggc agatagattt cgcatggcca atatgcagat
    21961 agccattcgg ctccggcggg aaacgggtgt gtactgtgat gtgcttacca ctggccagat
    22021 cttcatcgat gatctgacgg ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg
    22081 attcctcaaa gcgtaaacaa cgtataacgg cgtatgatct tataagcggg acaggctgac
    22141 aactgttagt tttttaaaat tgataatcta acaaggatta tggggatgat tgatgaaaaa
    22201 aaaagcggca gagaagatct ccaccgcttg tcattgttgg atgcgacgct gccgtgtcgc
    22261 atcaggcata aagcagatta ctttttgatt tcatacagcg gtgtttgacc cgccacaaca
    22321 tggccctgag ctttaatgag caagccactg aaatcgtcga tattgctgca aaccaccggg
    22381 ctaatcatcg agcgggcgtt agcgttcagg taatccagat ccatttccag aatcggttgc
    22441 cctgcgctta cctgcgcacc ctcttccacc agacgtttaa agcctttacc ttccagcgct
    22501 acggtgtcga tacccatatg gacgacgatc tccgcgcctt tttcggtttc caggcagaac
    22561 gcgtggttgg tgttgaagat tttcacgatt gtccctgcgg ctggtgatac gacgatttta
    22621 tctgtcggtt tcaccgccac accgtcaccc accgctttgc tggcgaatgc ttcgtcagga
    22681 acctgatcca gtgccacgac atcaccggta atcggcgata ccagctccgc gatagatacc
    22741 gcgtttggta cagcctgcgg ttttgctaca ggcgcggcag ttgccggagt tgcttcagct
    22801 gacgcagcgg ctaccggacc acgggcaacg actttcttca tcgcatcgcc gatggattct
    22861 gctttcgcgc caacaatcac ctgaatagtc tgtttgttca gtttcactac accagaagca
    22921 cccagacgtt tacacatcgt atcgttgacg cgggcagagt cagccacggt caggcgcaga
    22981 cgggtaatac aggcatcaat cgctttcagg ttgtcagtac cgccaaccgc agcaatatag
    23041 ttggttgcca gttgagtcag accttcttca gtattgctgt tggcttcttc agtaacgatc
    23101 tcgtcttctt tatcttcacg acccggcgtt ttcaggttga acatgcggat aaccaaactg
    23161 aacaccacga agtagatagc gaagaagata acgcccatca ccagcagcat ccagacgttc
    23221 tggctggcgg caggcaggtt atacatcaac gcgtagtcga tagcacccgc agagaaagag
    23281 aagccggcat ggatacccag cagcgttgcc acaaacaggc tgataccggt cagcagtgcg
    23341 tgcaggaggt acagcagcgg cgcaaggaac atgaacagga attccagcgg ctcagtcaca
    23401 ccggtcagga acgcagtaac agcaacagaa agcagcatcc cgccaaccat cggacgacgc
    23461 tctttcggtg ctgcgaagta catcgccagc gccgcacccg gcagaccgaa catcatgatc
    23521 gggaagaagc cggacatgaa catccccgcg gtgccgtcac cagcgtagaa acggttgatg
    23581 tcgccgtgga aaaccgtacc cgccgcgttg gtgaattcac caatctggaa ccaggcgatg
    23641 gtgttcagca cctgatgcag accggttggg atcagcagac ggttgatgaa accaaagata
    23701 ccggaaccca gcgcgcccgc agaaacgatc cactcgccgc ctgcatggat agcgtgctgt
    23761 accggcggcc agacgtaacc aaaaatggcc gccagcacca gacagaagaa gccggtggcg
    23821 atcggcacaa agcgtttgcc gccgaagaag ctcaggaagt ccggcagttt aatatcggac
    23881 caacggttat aggctgcgcc accaaccaga ccggtaatga tacccgccag tacacccatg
    23941 ttaatttctg ggttgatggt caccatcgct ttggttaaca caaagtaacc taccgcaccc
    24001 gccagcgccg ccgcacctgc gctgtctttc gaccagctgg atgccacacc gatggcgaag
    24061 attaatgcga ggttatcaaa aattgcaccg cccgcctggg caataaacgc aacgttaagt
    24121 aaatctggct gaccgaatcg cagcaacagc gccgccaccg gcagcaccgc gatagggagc
    24181 tgtaacgccc taccgagtcg ctggaaaaaa cctaaaatat tcatcttatt ccccctacga
    24241 gaaccctatt tggctcgttt aaagccgtat ttttattttg ctgcaaattt tactgccgat
    24301 gttctgtaat cagattgtta gatcatctgc tacagagtgt gtgaaaattt aattcgcatc
    24361 gcaaattaaa cgcgtgtctt ttgtgagttt tgtcaccaaa tatcgttatc atcactccct
    24421 tttactggct aaaccagaaa acttatttta tcattcaaaa aatcaagtcg gattgacgcc
    24481 tgtctgcgca aatccaggtt acgcttaaag atgtctaatc cgccaacggc ttacatttta
    24541 cttattgagg tgaataatga gactgatccc cctgactacc gctgaacagg tcggcaaatg
    24601 ggctgctcgc catatcgtca atcgtatcaa tgcgttcaaa ccaactgccg atcgtccgtt
    24661 tgtactgggc ctgccgactg gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat
    24721 gcataaagca ggccaggtca gctttaagca cgttgtcacc ttcaacatgg acgaatatgt
    24781 cggtctgccg aaagagcatc cggaaagcta ctacagcttt atgcaccgta atttcttcga
    24841 tcacgttgat attccagcag aaaacatcaa ccttctcaac ggcaatgccc cggatatcga
    24901 tgccgagtgc cgccagtatg aagaaaaaat ccgttcttac ggaaaaattc atctgtttat
    24961 gggcggtgta ggtaacgacg gtcatattgc atttaacgaa ccggcgtctt ctctggcttc
    25021 tcgtactcgt atcaaaaccc tgactcatga cactcgcgtc gcgaactctc gtttctttga
    25081 taacgatgtc aatcaggtgc caaaatatgc cctgactgtc ggtgttggta cactgctgga
    25141 tgccgaagaa gtgatgatta tggtgctggg tagccagaaa gcactggcgc tgcaggccgc
    25201 cgttgaaggt tgcgtgaacc atatgtggac catcagctgt ctgcaactgc atccgaaagc
    25261 gatcatggtg tgcgatgaac cttccaccat ggagctgaaa gttaagactt taagatattt
    25321 caatgaatta gaagcagaaa atatcaaagg tctgtaattg ttatccctgc cctggttcct
    25381 tgctcagggc aatatttttt aaaatcgggg ggcagaatgt atgcattaac ccagggccgg
    25441 atctttaccg gccacgaatt tcttgatgac cacgcggttg ttatcgctga tggcctgatt
    25501 aaaagcgtct gtccggtagc ggaactgccg ccagagatcg aacaacgttc actgaacggg
    25561 gccattctct cccccggttt tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt
    25621 aacgacaccg ctgaagcggt cagcgtggaa acgctggaaa tcatgcagaa agccaatgag
    25681 aaatcaggct gtactaacta tctgccgacg cttatcacca ccagcgatga gctgatgaaa
    25741 cagggcgtgc gcgttatgcg tgagtacctg gcaaaacatc cgaatcaggc gttaggtctg
    25801 catctggaag gtccgtggct gaatctggta aaaaaaggca cccataatcc gaattttgtg
    25861 cgtaagcctg atgccgcgct ggtcgatttc ctgtgtgaaa acgccgacgt cattaccaaa
    25921 gtgaccctgg caccggaaat ggttcctgcg gaagtcatca gcaaactggc aaatgccggg
    25981 attgtggttt ctgccggtca ctccaacgcg acgttgaaag aagcaaaagc cggtttccgc
    26041 gcggggatta cctttgccac ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa
    26101 ccgggcctgg cgggcgcgat cctcgacgaa gctgacattt attgcggtat tatcgctgat
    26161 ggcctgcatg ttgattacgc caacattcgt aacgctaaac gccttaaagg cgacaaactg
    26221 tgtctggtta ccgacgccac cgcgccagca ggtgccaaca ttgaacagtt catttttgcg
    26281 ggtaaaacaa tatactaccg taacggactt tgtgtggatg agaacggtac gttaagcggt
    26341 tcatccttaa ccatgattga aggcgtgcgt aatctggtcg aacattgcgg tatcgcactg
    26401 gatgaagtgc tgcgtatggc gacgctctat ccggcgcgtg cgattgacgt tgaaaaacgt
    26461 ctcggcacgc tcgccgcagg taaagtagcc aacctgaccg cattcacacc tgattttaaa
    26521 atcaccaaga ccatcgttaa cggtaacgag gtcgtaactc aataagagaa agtatgacac
    26581 caggcggaca agctcagata ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg
    26641 tttatcgcct gattgaccag tacgggccaa tctcgcggat tcagattgcc gagcaaagcc
    26701 agcttgcccc cgccagcgta accaaaatta cgcgtcagct tatcgaacgc gggctgatca
    26761 aagaagttga tcagcaggcc tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa
    26821 cccgcaattt ccacgcaatc ggcgtacggc ttggtcgtca tgacgccacc atcactctgt
    26881 ttgatctcag cagcaaagtg ctggcagaag aacattaccc gctgccggaa cgtacccagc
    26941 agacgctgga acatgccctg ctgaatgcca ttgctcagtt tattgatagc taccagcgca
    27001 agctgcgcga gctaatcgcc atttcggtga tcctgccagg gcttgttgac ccggacagcg
    27061 gcaaaattca ttacatgccg catattcagg tagaaaactg ggggctggta gaagcactgg
    27121 aagaacgttt taaagtgacc tgtttcgttg gtcacgatat ccgtagtctg gcgctggcgg
    27181 agcactactt cggtgcaagt caggattgcg aagactccat tctggtgcgt gtccatcgcg
    27241 gaaccggggc cgggattatc tctaacgggc gcatttttat tggccgcaac ggtaacgtcg
    27301 gtgaaattgg ccatattcag gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg
    27361 gctgcctgga aactatcgcc gccaacgctg ccattgaaca acgggtgttg aatctgttaa
    27421 agcagggcta ccagagccgt gtgccactgg acgactgcac catcaaaact atctgcaaag
    27481 ccgcgaacaa aggcgatagc ctggcctcgg aagtgattga gtatgtcggt cgtcatctgg
    27541 gtaaaaccat cgccattgct atcaacttat ttaatccgca aaaaattgtt attgctggtg
    27601 aaatcaccga agccgataaa gtgctgctcc ctgctattga aagctgcatt aatacccaag
    27661 cgctgaaggc gtttcgtact aatctgccgg tggtacgttc tgagctggat caccgctcgg
    27721 caatcggcgc ttttgcgctg gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt
    27781 tgctggaaaa ttaatgtgct tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc
    27841 catgaccatt aaaaatgtaa tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc
    27901 cgtaccgggt gcagcggaat ttttgcacgg gattatggat aaaggcctgc cgctggtgtt
    27961 gctgaccaac tatccttcgc agactgggca agatctggcg aaccgctttg ccactgccgg
    28021 tgtcgatgtg ccggatagcg tgttttatac ctctgcgatg gcgaccgccg atttcctgcg
    28081 tcgctaggaa ggcaagaaag cgtatgtggt gggcgaaggc gcactgattc atgaactgta
    28141 caaagccggt ttcactatta ccgatgtgaa ccctgatttc gtgattgttg gcgaaacacg
    28201 ttcctacaac tgggacatga ttcataaagc agcctatttc gtcgctaacg gtgcacgctt
    28261 tatcgccacc aacccggaca cccacgggcg cggtttttat cccgcttgtg gcgcgttgtg
    28321 tgcagggatt gagaaaatct ccgggcgcaa accgttctat gttggtaagc ccagcccgtg
    28381 gatcatccgc gcggcattaa acaaaatgca ggcgcattcg gaagaaacag tgattgtcgg
    28441 cgataacctg cgtaccgata ttctggcggg cttccaggcc ggtctggaga cgattctggt
    28501 gctttctggt gtttcatcgc tcgacgatat cgacagtatg ccgttccgcc caagctggat
    28561 ttacccgtca gtcgctgaaa tcgacgttat ctgaatacca cccacgtctg cggacgtggt
    28621 tggctctgat gacaaacgcc aaactgcctg atgcgctacg cttatcaggc ctacgcagct
    28681 cctgcaatat attgaatttt catgcttttg taggccggat aaggcgttca cgccgcatcc
    28741 ggcatgaaca aatcgcactt tatcagcaat ttggcccccg taaaatataa ccattcaata
    28801 aaacccgcta aaaattatcg attcatcaat aaatacatcc tcacaccaga ccctttttca
    28861 ccaatcgaca atcaccatta cgttttttat ttttgcactg ccaaatagct tgcgcacagt
    28921 gctgctttgc ggcatttttt taaacaagca aacacaacaa gcaacaaata ccaggttaac
    28981 ggagaaggtt atgtgttcaa tttttggcgt attcgatatc aaaacagacg cagttgagct
    29041 gcgtaagaaa gcactcgagc tgtcacgcct gatgcgtcat cgtggcccgg actggtccgg
    29101 tatttatgcc agcgataacg ccattctcgc tcacgaacgt ctgtcaattg ttgacgttaa
    29161 cgcgggggcg caacctctct acaaccaaca aaaaaaccac gtactggcgg taaacggtga
    29221 aatctacaac caccaggcat tgcgcgctga atatggcgat cgttatcagt tccagacagg
    29281 gtctgactgt gaagtgatcc tcgcgctgta tcaggaaaaa gggccggaat ttctcgacga
    29341 cttgcagggc atgtttgcct ttgccctgta cgacagcgaa aaagatgcct acctgattgg
    29401 tcgcgaccat ctggggatca tcccactgta tatggggtat gacgaacacg gtcagctgta
    29461 tgtggcctca gaaatgaaat cgctggtgcc agtttgccgc acgattaaag agttcccggc
    29521 ggggagctat ttgtggagtc aggacggcga aatccgttct tattatcatc gcgactggtt
    29581 cgactttgat gcagtgaaag ataacgtgac cgacaaaaac gagctgcgtc aggcactgga
    29641 agattccgtt aaaagccatc tgatgtctga tgtgccttac ggtgtgctac tttctggtgg
    29701 tctggattcc tcaattattt ccgctatcac caagaaatac gcagcccgtc gcgtggaaga
    29761 tcaggaacgc tctgaagcct ggtggccgca gttacactcc tttgctgtag gtctgccggg
    29821 ttcaccggat cttaaagcag cccaggaagt ggcaaaccat ctgggcacgg tgcatcacga
    29881 aattcacttc actgtacagg aaggtctgga tgccatccgc gacgtgattt accacatcga
    29941 aacttatgat gtgaccacta ttcgcgcttc aacaccgatg tatttaatgt cgcgtaagat
    30001 caaggcgatg ggcattaaaa tggtgctgtc cggtgaaggt tctgatgaag tgtttggcgg
    30061 ttatctttac ttccataaag cgcccaacgt taaagaactg catgaagaga cggtgcgtaa
    30121 actgctggcc ttgcatatgt atgactgcgc gcgcgccaac aaagcgatgt cagcctgggg
    30181 cgtggaagca cgcgttccgt tcctcgacaa aaaattcctt gatgtggcga tgcgcattaa
    30241 cccgcaggat aaaatgtgcg gtaacggcaa aatggaaaaa cacatcctgc gtgaatgttt
    30301 tgagtcatac ctgcccgcaa gcgtggcctg gcggcagaaa gagcagttct ccgatggcgt
    30361 cggttacagt tggatcgaca ccctgaaaga agtggcggcg cagcaggttt ctgatcagca
    30421 actggaaact gcccgcttcc gcttcccgta caacacgccg acctcaaaag aagcgtatct
    30481 gtaccgcgag atctttgaag aactgttccc acttccgagt gccgctgagt gcgtgccggg
    30541 cggtccttcc gtcgcgtgtt cttcagctaa agcgatcgaa tgggatgaag cgttcaaaaa
    30601 aatggacgat ccgtctggtc gtgcggttgg cgttcaccag tcggcgtata agtaagaaat
    30661 gcggtactgc cccggagaaa tccggggctg ttttagttat tgcgctgctt gttgattctg
    30721 atatgcagta aggaaatgtt ccgggtttgc cacatcactg tttgacaact catcactgta
    30781 ggccagtatt tcatagatta atttcccacc actattgttg aattccgaat ttcatcctgt
    30841 acgacaaaag aatggctgta gggtggggaa aaacacaaag taaacaataa ttgacgaata
    30901 tagcgccacg ctgttcgcaa cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa
    30961 agaggttgac gctgcaaggc tctatacgca taatgcgccc cgcaacgccg ataaggtatc
    31021 gcgaaaaaaa gatggctacg tagctcagtt ggttagagca catcactcat aatgatgggg
    31081 tcacaggttc gaatcccgtc gtagccacca tcttttttgc gggagtggcg aaattggtag
    31141 acgcaccaga tttaggttct ggcgccgcaa ggtgtgcgag ttcaagtctc gcctcccgca
    31201 ccattcacca gaaagcgttg tacggatggg gtatcgccaa gcggtaaggc accggttttt
    31261 gataccggca ttccctggtt cgaatccagg taccccagcc atcttcttcg agtaagcggt
    31321 tcaccgcccg gttattgggg tatcgccaag cggtaaggca ccggtttttg ataccggcat
    31381 tccctggttc gaatccaggt accccagcca tcgaagaaat aatctggcta cgtagctcag
    31441 ttggttagag cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac
    31501 caaattctga atgtgtcgaa tacgttcggc aaattcaaaa accaatttgt tggggtatcg
    31561 ccaagcggta aggcaccgga ttctgattcc ggcattccga ggttcgaatc ctcgtacccc
    31621 agccaattta ttcaagacgc ttacatcgta agtgcaccca gttggggtat cgccaagcgg
    31681 taaggcaccg gattctgatt ccggcattcc gaggttcgaa tcctcgtacc ccagccacat
    31741 taaaaaagct cgcttcggcg agctttttgc ttttctgcgt tcattcaatg tcgaatgcga
    31801 tgttgacacg tcttatcctt caatgtcgga tgcgacgctg ccgcgtctta tccgacctac
    31861 ggctggcacg catccggcaa tgttgtaagg ctacaaccct aacgcatatt tcagcgcctg
    31921 acgtttcaac acgccagcac gctctgccgc cattaacccg agattacgca caaaacgcag
    31981 cggcggcaga ttattactga atccggcata aaacagatcc ataccacttt gcataatgaa
    32041 gttatccgcc atgcgccgca tctggtaacg cttgaggaca ggataactgg cccacgcttc
    32101 accgtagctg cgggcattca ccagaacatc aatcagggca tcgacatcac gataaccaag
    32161 attcaccccc tgccccgcca gcggatggat ggtatgcgcg gcatcgccca ccagcgcaag
    32221 ccccggctgc acatactgca acgcatgtcg gcgcgtcagc ggaaacgcac cagcggcaag
    32281 cggtgtaacg taacccagac gcgacgggaa atgcttcgcg atttccgcct ggagttgcgc
    32341 catattcata ttctgcaact ggcgaatacg cgtcggagag tcataccaca ccagcgatgc
    32401 ccagttatca aacaacggca gaaacgcacg cggtccgtcc ggggtaaatt gctgccaggt
    32461 gctgtcgccg ggatcgttct cacactgtac gctaatcaac atgcacgact gcgcatactg
    32521 ccaggcatga acgccaattc ccgccatctg ccgcacctga gaatttgcgc cgtcggcacc
    32581 aatcaccagt ttcgcgcgga tcgtttcacc gcccttcagt tccagctcct gaagatcatt
    32641 atctcgatgt aatgcaatca gcgaggttgg cacacgtaac gttactttcg gatgcgcttc
    32701 cagcgcctgc cacaacgcct gttgcaggac tgtgttttcc accatgtagc caagcaacgg
    32761 caactttaat tcggctgcgt caaataccac atgtgccgtt tcccactccc aggtttccag
    32821 tctgcggtaa ggatggcaac gcatagcctg tactgcatcc cagaccccta accctttaag
    32881 caatgatacc gaagccgcgc tgatcgccga gatccgcacg tccggttggc tgtcagcgac
    32941 aaacggtgca ggttctgcgt gctcgatcac cgttaccgaa aatccgtgct gtgccagccc
    33001 cagcgccagt gcgccgccga ccattcctcc gccgacaatg gcaatttccg ttggttgatt
    33061 tgtcatggtc aatcatcctg ttagcaatat gctttaagtt tacaggattt ttacccccaa
    33121 gggctgataa cgctcatact ggtcacaacg gcagcaaagc attacactat gcgccctgca
    33181 ttcctggcta ctatttcgca agagcaagtc gatgaccaaa aaactccata ttaaaacctg
    33241 gggctgtcag atgaacgagt acgattcatc gaagatggcc gatctgctgg atgccaccca
    33301 cggctatcaa ctgaccgacg tggcggaaga agcggatgtg ctactgctga acacctgctc
    33361 aatccgcgag aaggctcagg aaaaagtctt ccatcagttg ggtcgctgga aactgttaaa
    33421 agagaagaat ccagacctga ttatcggcgt cggtggctgc gtggcatcgc aagaaggcga
    33481 gcacattcgc cagcgcgccc actatgtcga tattattttt gggccgcaaa cgctgcaccg
    33541 tctgccggag atgatcaact ccgtgcgcgg cgaccgcagc ccggttgtag atatcagctt
    33601 cccggaaatc gagaagtttg accgtctgcc ggaaccgcgc gccgaagggc cgaccgcgtt
    33661 tgtctccatc atggaaggct gcaataaata ttgcacctac tgcgtggtgc cttacacccg
    33721 tggtgaagag gtaagccgtc cgtctgacga tattctgttt gagattgccc agcttgcggc
    33781 tcagggcgtg cgtgaagtca acctgctcgg tcagaatgtg aatgcctggc gtggtgagaa
    33841 ctacgacggc accaccggat cgtttgccga tctgttgcgt ctggttgctg cgatcgacgg
    33901 gatcgatcgt attcgcttta ccaccagcca tccgatcgaa ttcaccgacg atatcatcga
    33961 agtgtaccgc gacacgcctg agctggtgag cttcctgcat ctgccggtac agagcggttc
    34021 cgatcgcatt ctgaacctga tgggacgtac ccatacggcg ctggagtaca aagcgatcat
    34081 ccgtaaactg cgtgcggcgc gtccggatat tcagatcagt tctgacttca ttgttggctt
    34141 ccctggcgaa accaccgaag acttcgagaa aacgatgaag ctgattgccg acgtcaattt
    34201 cgacatgagc tacagcttta tcttctctgc acgtccagga acgcctgccg ccgatatggt
    34261 tgatgatgtt ccggaagaag agaagaagca gcgtctgtat attctgcaag aacgtatcaa
    34321 tcagcaagcg atggcatgga gccgtcggat gctcggcacc acccagcgta ttctggtaga
    34381 agggacttcg cgtaagagca tcatggagct ttccgggcgt accgaaaata accgcgtggt
    34441 caacttcgag ggcacaccgg atatgatcgg taaattcgtc gatgtagaaa ttaccgacgt
    34501 ctacccgaac tctctgcgcg gtaaagtggt gcgaactgaa gatgaaatgg gtctgcgcat
    34561 ggcagaaaca ccggagtcgg tgattgcccg cacccgcaaa gaaaacgacc ttggcgtggg
    34621 ttattatcag ccgtaattct caggcctgcc gttccggtgg gccttgtttt ttctctcttt
    34681 atccccatct ttatcgcaat gcttgcgcct ttttcctgtg gcgtgaataa tttccttata
    34741 ggccggtgaa tgttcagctt ccggcggcat acagataaga ggaacggttt gaacatagac
    34801 actcgcgaaa tcaccctgga gccagcagac aatgcgcgtc tgttgagcct gtgcggcccg
    34861 tttgatgaca acatcaagca gctcgaacgc cgtctcggca tcgagatcaa tcgccgcgat
    34921 aaccacttta aactgaccgg ccgtccgatt tgcgtcaccg ctgcggcaga cattctgcgt
    34981 agcctgtatg tcgatactgc cccgatgcgc ggtcagattc aggatatcga accggaacag
    35041 atccaccttg cgattaaaga agcgcgggta ctggagcaaa gcgcggagag cgtgccggag
    35101 tacggcaaag cggtcaatat caaaactaaa cgcggcgtaa ttaagccgcg cacgccaaac
    35161 caggcgcagt acatcgccaa tattctcgac catgacatta ccttcggcgt tggcccggcg
    35221 ggtacgggta aaacctacct ggcagtggct gcggcagttg atgccctgga gcgtcaggaa
    35281 attcgccgta ttctgctgac tcgtccggcg gtcgaagccg gtgagaaact gggcttcctg
    35341 cctggcgatt taagccagaa agtagacccg tatctgcgcc cactgtacga cgcgctgttt
    35401 gaaatgctgg gctttgagaa agtcgagaaa ctgattgagc gcaacgttat tgaagtcgcg
    35461 ccgctggcct atatgcgtgg tcgtacgctg aacgacgcgt ttatcattct cgatgagagc
    35521 cagaacacta ccatcgaaca gatgaagatg ttcctgaccc gtatcggttt taactcaaaa
    35581 gcggttatca ccggcgacgt cacacagatc gacctgccgc gtaatactaa atcaggctta
    35641 cgtcatgcca ttgaagtgct ggccgatgtc gaagagatca gctttaactt cttccacagc
    35701 gaagacgtgg ttcgtcaccc ggtggtggcg cgtatcgtta acgcttatga agcctgggaa
    35761 gaagcagaac aaaaacgtaa agcggcgctg gcagcagaac gcaagcgcga agaacaggaa
    35821 caaaaatgag tcaggtgatc ctcgatttac aactggcatg tgaagataat tccgggttac
    35881 cggaagagag ccagtttcaa acatggctga atgcggtgat cccgcagttt caggaagagt
    35941 cggaagtgac tattcgcgtg gtcgataccg ccgaaagcca cagtctgaat ctgacctatc
    36001 gcggtaagga taagccgacc aacgtgctct ccttcccgtt tgaagtgccg cctggcatgg
    36061 agatgtcgct actgggcgat ctggttatct gccgtcaggt ggttgagaag gaagctcagg
    36121 agcaaggcaa accactggag gcgcactggg cgcatatggt ggtgcacggc agtctgcatt
    36181 tgttaggtta cgatcacatt gaagatgacg aagcagaaga aatggaagcc ctcgaaacag
    36241 agattatgct tgctctgggc tatgaggatc cgtacattgc cgagaaagaa taatttgcca
    36301 ggcatttgac tggcagacgt ccccgccgtt ggtgattaac gacggcgttt tgattaacta
    36361 acatgagaac ccatacgacg ccatgagcga cgacaattca cacagtagtg acacgataag
    36421 caacaagaag ggatttttct ccctgttact cagccaactt ttccacggtg aaccgaaaaa
    36481 ccgtgacgaa ctgctggcgc tgatccgtga ttccgggcag aacgacctta tcgacgaaga
    36541 tacgcgcgat atgctcgaag gggtgatgga catcgcagac caacgcgttc gcgacatcat
    36601 gatcccccgc tcccagatga ttaccctgaa acgcaaccag acgctggacg aatgccttga
    36661 tgtcatcatc gagtccgccc actcacgttt cccggtgatt agcgaagaca aagatcacat
    36721 tgaagggatt ctgatggcga aagacttgct gccgtttatg cgcagcgatg ctgaagcctt
    36781 cagcatggac aaagtgttac gtcaggcggt tgtcgttcct gaaagtaagc gcgtagaccg
    36841 gatgctgaaa gagtttcgct ctcagcgtta ccacatggcg atcgttattg acgaattcgg
    36901 tggggtttcc ggtctggtga ccattgaaga catcctggaa ctgattgttg gtgagattga
    36961 agacgagtat gacgaagaag atgatatcga cttccgtcag ctgagtcgtc atacctggac
    37021 cgtgcgcgca ctggcttcca ttgaagactt caacgaagcg ttcggcaccc actttagcga
    37081 tgaagaagtc gacactatcg gtggtctggt gatgcaggcg tttgggcatc ttccggcgcg
    37141 tggcgaaact atcgacatcg acggttacca gttcaaagtg gcgatggccg atagtcggcg
    37201 tattattcag gttcatgtca aaatcccgga tgactcaccc cagccaaagc tggatgaata
    37261 aaaccgaaac tggatagata actacatgga ttttgcctca ttaattgaac gccagcgcat
    37321 tcgcctgctg ctggcgttat tattcggtgc ctgcggaacg ctggccttct ctccttacga
    37381 cgtctggcct gcggcgatta tttcgctgat ggggcttcag gcgttgacct ttaaccgtcg
    37441 cccactccag tctgccgcta ttggcttttg ctggggattt ggcctctttg gcagcggtat
    37501 taactgggtc tatgtcagca tcgcgacctt tggcgggatg cctggcccgg ttaacatctt
    37561 cctggtggtg ctgctggcgg cgtatttgtc gctgtatacc ggactgtttg ctggcgtgct
    37621 gtcgcgtctg tggccgaaaa ccacctggct gcgcgtggcg attgccaccc ctgctctctg
    37681 gcaagtgacc gagtttctgc gcggttgggt gctgactggc ttcccgtggt tacagttcgg
    37741 ctatagccag attgatggcc cgttaaaagg gctggcaccg ctaatgggcg tggaagccat
    37801 taacttcctg ctgatgatgg ttagtggcct gctggcactg gcgttggtca aacgcaactg
    37861 gcgtccgctg gtggtggccg tcgtgctgtt tgccctaccc ttcccgctgc gttacatcca
    37921 atggtttacc ccgcaaccgg agaaaaccat tcaggtttcg atggttcagg gcgatattcc
    37981 gcaatcgctg aaatgggacg aaggccagct tcttaatacg ctaaagattt actacaacgc
    38041 aacggcaccg ctgatgggca aatcatcgtt gattatctgg ccggagtcgg cgataaccga
    38101 tctggaaatt aatcagcaac cgttcctcaa agcactggac ggtgagttgc gtgataaagg
    38161 tagctcgctg gtaaccggga ttgtcgacgc gcgtctcaat aagcagaacc gctacgatac
    38221 ctacaacacc atcatcacgc tgggtaaagg tgcaccgtac agctacgaat cagccgatcg
    38281 ctataacaaa aaccatctgg tgccgtttgg cgagtttgtc ccgctggagt cgattctgcg
    38341 tccgttagca ccgttctttg atctgccgat gtcgtcgttc agccgtgggc catatatcca
    38401 gccgccgctg tcggcaaatg gtattgagct tactgcggct atttgctacg agattattct
    38461 cgccgagcaa gtgcgcgata acttccgccc ggataccgac tacctgctga ctatctccaa
    38521 cgatgcgtgg tttggtaaat ctattggtcc atggcaacac ttccagatgg cgcgaatgcg
    38581 tgcgctggag ctggcgcgcc cactgttgcg cagcaccaac aacggcatta cggcggtgat
    38641 tggcccgcag ggtgagattc aggcgatgat cccgcagttc gcccgcgagg tgttaaccac
    38701 taacgtgacg ccgaccaccg gactcacacc atacgcacgt accggcaact ggccgctgtg
    38761 ggtgctgacg gcactgtttg gttttgctgc tgtgttgatg agtctgcgtc agcgacgtaa
    38821 ataatccctt cttatcgtgc cggaatacat cccatatttc ggcacgctca ccggatgcgg
    38881 catcagtgca ttatccggat aacaatattc tcctatccaa ctttggcaca tctattgctt
    38941 tgttatacaa ggcaaagctt gaaccagcat cagtacaacc cggttgcacc acagcaatcc
    39001 accggtagca ccaaaagagt gcattttaag aattttgctt cagaatggtg cggtgcacct
    39061 cgcaaaaaca aacaataaaa catcaatatc tttacattta gccaaactaa atgttaccta
    39121 acaggcacaa cactgcacaa taaagttgca gacgataaca acacaaacac tcacaacggg
    39181 tatccatgcg ttcttaacgc agaagataaa ggtgttggat atgcaattac gtaaacctgc
    39241 cacagcaatc ctcgccctgg cgctttccgc aggactggca caggcagatg acgccgcccc
    39301 ggcagcaggc agtacgctgg acaaaatcgc caaaaacggt gtgattgtcg tcggtcaccg
    39361 tgaatcttca gtacctttct cttattacga caatcagcaa aaagtggtgg gttactcgca
    39421 ggattactcc aacgccattg ttgaagcagt gaaaaagaaa ctcaacaaac cggacttgca
    39481 ggtaaaactg attccgatta cctcacaaaa tcgtattcca ctgctgcaaa acggcacttt
    39541 cgattttgaa tgtggttcta ccaccaacaa cgtcgaacgc caaaaacagg cggctttctc
    39601 tgacactatt ttcgtggtcg gtacgcgcct gttgaccaaa aagggcggcg atatcaaaga
    39661 ttttgccgac ctgaaaggca aagccgtagt cgtcacttcc ggcactacct ctgaagtttt
    39721 gctcaacaaa ctgaatgaag agcaaaaaat gaatatgcgc atcatcagcg ccaaagatca
    39781 cggtgactct ttccgcaccc tggaaagcgg tcgtgccgtt gcctttatga tggatgacgc
    39841 tctgctggcc ggtgaacgtg cgaaagcgaa gaaaccagac aactgggata tcgtcggcaa
    39901 gccgcagtct caggaagcct acggttgcat gttgcgtaaa gacgatccgc agttcaaaaa
    39961 gctgatggat gacactatcg ctcaggtgca gacctccggt gaagcggaaa aatggtttga
    40021 taaatggttc aaaaatccaa ttccgccgaa aaacctgaac atgaatttcg aactgtcaga
    40081 cgaaatgaaa gcactgttca aagaaccgaa tgacaaggca ctgaactaat tacaagaacc
    40141 aggggcggaa attccagccc tctcgattgt tacgtagcac ggacagacta tacgcctgat
    40201 ggtcgttccc catcgggcct gaaaaccgca atacgctggg taacaatctt cgagggtagc
    40261 agttaacgct gctaccctct tttttctgga gtagatttat gtctatagac tggaactggg
    40321 gtattttttt acaacaagcc ccgttcggca ataccaccta tcttggttgg gtctggagtg
    40381 gttttcaggt gacgatcgct ttgtcgatct gcgcctggat tatcgctttc ctcgtcggtt
    40441 catttttcgg cattttacgt accgttccaa accgttttct ctccggtctg ggtacgttgt
    40501 atgtcgaact gttccgcaac gtgccgttga ttgtgcaatt ctttacctgg tatctggtga
    40561 tcccggagct gctgccggag aaaatcggca tgtgatttaa ggccgagctg gatcccaata
    40621 ttcagttttt cctttcatcc atgctctgcc tggggctgtt taccgccgcc cgtgtttgcg
    40681 aacaggtacg cgccgctatt cagtcgctac cgcgtgggca gaagaatgcc gcgctggcga
    40741 tgggcctgac gctaccgcag acttatcgat acgtactgct gcctaatgct tatcgcgtta
    40801 tcgtcccgcc gatgacttcc gagatgatga acctggtgaa aaactccgcc atcgcctcga
    40861 ccatcggtct ggtggatatg gcggcgcagg cgggtaaatt gctcgactac tcggctcacg
    40921 cctgggagtc gtttaccgcc atcacgcttg cgtatgtgtt gattaacgct tttatcatgc
    40981 tggtgatgac gctggttgaa cgtaaagttc gcctgcccgg caacatgggg ggcaaataat
    41041 gtacgagttt gactggagtt ccattgttcc ttccctgcca tatctgctcg acgggctggt
    41101 gatcaccctg aaaatcaccg tcacggcagt cgtgattggt attttgtggg gtaccatgct
    41161 ggcggtgatg cgcttgtcca gcttcgcgcc tgtttcgtgg tttgccaaag cctacgttaa
    41221 cgtattccgc tcgattcctt tagtcatggt tttgctgtgg ttttacttga ttgtgccggg
    41281 atttctgcaa aatgtgctgg gattatcgcc aaaaaatgat attcgtttaa tttcggcgat
    41341 ggtggcgttt tcgatgtttg aagcggccta ctattcagag attatccgtg ccggtattca
    41401 aagtatttcc cgtggtcagt cgagcgccgc gctggcgttg gggatgactc actggcagtc
    41461 gatgaaactg attattctgc cgcaggcttt ccgcgcgatg gtgccgctgc tgctcactca
    41521 gggcatcgta ctgttccagg atacctcgct ggtgtatgtg ttatctctgg ccgatttctt
    41581 ccgtaccgcc tcaaccattg gtgagcgtga tggtacgcag gtcgagatga tcctgtttgc
    41641 cggatttgtt tatttcgtta ttagccttag cgcgtcgttg ttggtcagct acttgaaaag
    41701 aaggacagca taatgattac cctgaaaaat gtttcaaaat ggtatggtca ctttcaggtg
    41761 ctgaccgact gctcaaccga agtgaaaaaa ggcgaagtgg tggtggtttg cggcccgtct
    41821 ggttccggca aatcaacgct gattaaaacc gtcaacggcc tcgaaccagt gcagcaaggt
    41881 gaaatcaccg tcgatggtat cgtggttaac gacaagaaaa ccgatctggc aaagctgcgc
    41941 tcccgcgtcg ggatggtatt ccagcatttc gagctgttcc ctcatctgtc gattatcgaa
    42001 aacctgaccc tggcgcaggt gaaagtgctt aaacgcgata aagcgcccgc tcgtgaaaaa
    42061 gcattgaaat tgctggagcg cgtgggactt tccgcacatg ccaataaatt tccggcgcag
    42121 ctttccggcg gtcagcagca gcgcgtggca atcgctcgcg cattgtgtat ggctcctatt
    42181 gcgatgctgt tcgacgaacc gacatcggcg ctggatccgg agatgatcaa cgaagtgctg
    42241 gacgtgatgg tggaactggc gaacgaagga atgaccatga tggtggtgac ccacgaaatg
    42301 ggctttgccc gcaaagtggc gaatcgggtg atctttatgg acgagggtaa aattgtcgaa
    42361 gactcgccga aagacgcttt cttcgatgat ccgaaatcgg accgcgcaaa agacttcctc
    42421 gcgaaaatcc tgcattaatc actctggcgc acatacccaa ttgcgcgcca tctcacgctt
    42481 tatcgtttcg cccctcgtat ctgttacatc ctggcgttac ccttgtcgca aagaagcaca
    42541 caacaaggag caacaatggc actgccaatt ctgttagatt gcgacccagg tcatgatgac
    42601 gctatcgcaa tagttctcgc actcgcctca ccagagcttg atgtcaaagc aattacgtct
    42661 tccgccggaa accagacacc agaaaaaacc ttacgcaatg ttctgcgtat gctgaccttg
    42721 cttaatcgca ccgatattcc ggtggcaggc ggcgcggtaa aaccgttaat gcgtgagttg
    42781 attatcgcgg acaatgtgca cggcgaaagc ggtctcgacg gcccggcatt accggaaccg
    42841 acgttcgcac cgcaaaactg tacggcggtg gagctgatgg cgaaaacgct gcgcgaaagt
    42901 gcggaacctg tcacgattgt ttctaccgga ccgcaaacta acgttgcctt gctgctcaat
    42961 agccatccgg aactgcatag caaaattgcc cgtatcgtga ttatgggcgg cgcaatgggg
    43021 cttggtaact ggacgcctgc ggctgaattt aatatttacg ttgacccgga agcggcagaa
    43081 attgtcttcc agtcagggat cccggtggtg atggccggtc tggatgttac tcataaagcg
    43141 caaatccacg ttgaagacac cgagcgtttc cgcgcgattg gcaaccctgt ttcaaccatt
    43201 gttgccgaac tgctggattt cttcctcgaa tatcataaag acgaaaaatg gggctttgtc
    43261 ggcgcaccgc tgcatgaccc atgcaccatc gcctggctgt tgaaacccga gttgtttacc
    43321 actgttgagc gctgggttgg cgtggaaaca caggggaaat atacccaggg tatgacggtt
    43381 gttgattatt attatctgac tggcaataaa ccgaatgcca ctgtaattgt cgatgttgat
    43441 cgtcagggct ttgttgattt actggctgat cgtctgaaat tttacgctta acactcgtca
    43501 ttgattaaga tcaccacgcg ttacctgggt ggtcttaatc tatttatctc tttttctttc
    43561 aagatataaa tgcaatggat aatgcagaac tcgccatagg tatcgatctc ggcactacca
    43621 atagtttaat tgccgtctgg aaagacggtg ccgcgcaatt aattccaaat aagttcggtg
    43681 aatatttaac accatccata attagcatgg atgaaaataa tcatatttta gtcggaaaac
    43741 cggctgtatc acggcgtact tcgcatccgg ataaaacggc agcgttattt aaacgtgcaa
    43801 tgggcagtaa taccaactgg cggttaggca gcgacacatt taacgcgcca gaactgtcct
    43861 ctttggtatt acgctcatta aaagaagatg ccgaagaatt tctgcaacgt ccgattaaag
    43921 atgtggtgat ctccgttctg gcttatttca gcgatgaaca acgcaagcat acccgtttag
    43981 cagcggactt agccgggtta aatgcggtac gcttaattaa tgaacccaca gcagctgcga
    44041 tggcgtatgg cctgcatacc caacaaaata cccgttcgct ggtgtttgat ctcggtggcg
    44101 gcacgtttga cgttacggtg cttgagtacg ccacgccggt gattgaagtt cacgcctccg
    44161 ctggcgacaa cttcctcggt ggcgaagatt ttacccatat gttggtcgat gacgttttaa
    44221 agcgcgcgga tgtcacaagg accacgctta acgagagtga actggcagcc ttgtacgcct
    44281 gtgtggaagc ggcaaaatgt agcaatcaat cgccaatgca cattcgctgg tagtaccagg
    44341 atgaaacgcg ggaatgcgaa ttttacgaga acgaactgga agatttgtgg ttgccgctgc
    44401 tcaatcgctt gcgagtgccg attgaacagg cgttgcgcga tgcgcgtctg aagccgagtc
    44461 aaatcgacag tctggtgctg gttggcggcg cgtcacaaat gccgctggtg cagcgaatcg
    44521 ccgtgcgtct gtttggcaaa ttaccgtatc aaagttacga tccgagcacc attgtcgcgc
    44581 tgggcgcagc aatccaggcc gcctgccgct tacgcagtga agatattgaa gaagtgatcc
    44641 tcactgatat ttgcccttac tcgttgggcg ttgaggttaa ccgccagggc gtttccggca
    44701 ttttctcgcc gattattgaa cgaaacacca ctgtgcccgt gtcgcgtgta gaaacttatt
    44761 caaccatgca cccggaacag gattcaatta cggttaacgt ctatcaggga gaaaaccaca
    44821 aagttaaaaa caacattctg gtggaatcct tcgatgtgcc gttgaagaaa accggggctt
    44881 atcagtcgat tgatattcgc tttagttatg atatcaacgg gttgcttgaa gttgacgtgc
    44941 ttctggaaga cggcagcgtt aagtccagag tgattaacca cagcccggta acattgagcg
    45001 cgcagcagat tgaagagagt cggacgcggt tatccgcatt gaaaatttat ccgcgcgata
    45061 tgctcatcaa tcgcaccttt aaagccaaac tggaagagtt gtgggcgcgg gcgctgggtg
    45121 acgagcgaga agagattggc cgggtgatca ccgattttga tgcggcgctg cagtcaaacg
    45181 atatggcccg cgtcgatgag gtcaggcggc gggcgagcgt ttatttagcc attgagacct
    45241 cataatcacg gctgccggat gcggcgtaaa tgctgcatcc ggcatcggcg caaaaaacac
    45301 atcgtcaact aaacaacatc gcgattttgc cgagagcata acctataacc agcaccgtta
    45361 aactgatttt gataatgctg gtaatgctct gtggtttttc accctcatca tttttttctt
    45421 catggttttc aggtgttggc tcatcgacat cttcatcctc cggctcgagt tttactttcg
    45481 tcaacagcat ccgggtgata atggcctcta atggatgagc atctccgcct tccagccttg
    45541 tattaagacg ggccagcatc tcttgtttcg gaaacgcttc aagtactacc tgttccgcta
    45601 cccgatacaa atccagtaac ggtgaatctt cttctgtctc gctaccgact ctcgatttcc
    45661 accacgccca tgctttgcgt ttgaagaata acgactcaat aaacgattga cctaacggcg
    45721 ataattttcc gctatgtaaa atttccgccc ataacagccg cgtccgggcg ctctcaccag
    45781 gaacatattt cagcggttga ccttgcgcat ctaccatgct ccgcgcttcg ataaccatca
    45841 ctaataacgc gaaataatcc gggcaatgac gcttgctcca ctgcaacaac aaattatcag
    45901 cttgtgtaga tgggtaactt tcccagtacg cgccgagatc agcaagcagg ctttccccct
    45961 cgccgcagta caaacgttgt gcacactggt aataccgggc atcttcgctt tctggttctt
    46021 ccgcttgcca ctgtttggct acaggaatga gttccaccat acctgtttgc accgagctgt
    46081 accagcgcaa cagtttgcga tgtaactttg catcatcagg aatgaaccac ggtccttcca
    46141 tcgccaaaaa cgcagtgaca tagtcaggat ggtaacggca gcaacgttcc agcgcgaagt
    46201 aatattcaac agtctggttc tgtaacgcga cgggatattc cagaaggcta aggaaatcga
    46261 aacaatcacc agcctgaatg gcctcaagaa aagcctccct ttcttcctca tccattcctt
    46321 cgctgtcatt aaacgaatgc cagttcaaac gctctgccaa caaagaagca caatttaatg
    46381 acagatatcg cgcttctata gcgattgcac acagcggcca gcgtaattga tcgacatcct
    46441 ccatgttcca ggtatttaat tgctgaataa atttctgcca ggcggagggc tgaaaacgat
    46501 cactttctga atccagtaat gtcctgaatg cacgtagtat ttcatgttca gcggtggcat
    46561 ctttttcttc atcatctgct tcctcgacag gatttaccgc cagtcgcagc gcctcttcat
    46621 aggcctggcg taatgcttta aacccctgcg gatcggtttc gggatgacac aacggtaagc
    46681 gagcaagata agcctggcgg ataatgtcta tttgcgtcgt gctttcaatt tcgaggattt
    46741 gccaacatgt tttcataggt cgctcacatc cttaagcgtt accgggcatt caggctggac
    46801 agttaatggc tgccagggaa gcgtggcata gaaacggtcg cttcccgctg ccagctcttc
    46861 gaacatccga ctcccggcgc tatcgtattt gtacagggtt agcgcctcgc gtagcgaagc
    46921 gttgtcttcg cactgagaag attgccagta gagacgccct agtgaatagg cagaaaaata
    46981 gtgcatccag ctgtgatagt aatggtgagc tcgtgcatag acgcgcgatt gcagccataa
    47041 actctcttct tccgtcagcc agttgttgag cacaccggcc cgtgacagaa atcccattcg
    47101 aacataatcc caggctttga ttcccccttc tccacagcac atagcggttt gggcaacgaa
    47161 ttgggtataa atcaaccctc tctccgagcc accagcaatt aatgctttcc actcttcagg
    47221 ggaagagcgt tgccatgtca gatataagcc agctaaatca ttggcatgac catcatcagt
    47281 catctgataa atagtctgaa ttaaatcatc acgattattt attccccagg atgatgagag
    47341 atcgaattct tttatcccgg tatacatttt tggcgagtca tgcgcgggtt tgcaactctg
    47401 attaagtatt tccatgggcg cagaaagcgc aaaaagaaga tattgttctt ccttattcat
    47461 tttttaataa tcccttaagt gcattaactg actacttttt tattattgat gagtataaac
    47521 ttgacgcttt caaaataaaa agaaaatcga agcattcaca catgaataaa aaattaatgt
    47581 atatattcgc aatttttata gttgcagcaa ttacctgtat tagccaaccc aagaaaacga
    47641 tgttgcgtga taaagccatg gtgaattatg cctttgatta tttaagatca ccaggcagtc
    47701 taccgttaac cacggcagcc acggagcttt ccgcgattca tggtcactca acatcgcaat
    47761 atcgccttgg cgaattttat cttcatggta gcgacggtaa accactggat tatacacagg
    47821 cgagatactg gtatgagcaa tcagcagaac aggaaaatcc gcgcgcacaa agcaaactgg
    47881 ggtggatcta cctgaaaggc ctgggagtca aacccgatac ccgtaaagca attctctggt
    47941 ataaggaagc agctgaacaa gggtatgctc atgctcaata tactttgggt ttgatctaca
    48001 gaaatggcac tggtattaat gttaaccatt atgaatctca aaaatggtta aaactggccg
    48061 ccaaacaaca ttacaaaaat gcggaaagat tacttgccgg acttcccgca cattaacgtc
    48121 gactgtcacc cgaagagatg gagtattttc cctatcaaac cggcaaagat aaaaatgtag
    48181 aaaataattt tgatgacgcc cagacagccc agcccgccat catcaacggg cattggctgc
    48241 ggttgcggct ccggctcgac ggtttcctga ccggtaaaat tcgccgcctg aattaataat
    48301 cgacgaataa ttaatgcttc cagtggacaa cgatcttctg catcaaggcg gctcacaatc
    48361 ggcattaatg attcttctgt cggttggctg gcctgcacta attgtttggc aactaaatat
    48421 aatggccata agggatgttc tgtatcacgc gtgagtgaga tccgcggctt acgtcctttg
    48481 tgcaaaacca tctcgaccag tgaacgcccc agcgcagaga gtttttcatc gctgagtatt
    48541 tcgtataaat gaaaacgcgt acgggcgctg tcgccgggga cataaagtag cggttttccc
    48601 tgatcgttaa ccagatcacg cgcttcaatc atcatcacca ctaatgggta ataatagacc
    48661 cgatgctgac ggcaccattg caacattaaa gcatccgcct gggtggaggg atactcgcgc
    48721 cagtagtaac aaagttctgc cagcaggctt tcgccttctc cacaatagac gcgttgtgcg
    48781 tattcataat aacgcgcatc ttcattatca ggttcagctt cccgccacga ccgggcgtaa
    48841 tccaggagtt cggggattcc caggcggctt gcgctatacc agcggagtaa atttctgtgc
    48901 agttttgcat catcggggat aatcactgcg cgatgttgcg ctaaatacaa tgtcagccat
    48961 tctggatgcg atgaccaaat ccgagcgaac atttggtaaa aatcgatggt gtcattttgc
    49021 acggcaactg gcagatgcag aatggtctgg aagttaaaaa cattcccctt cgcaatggca
    49081 tataaaaagg attccagttc ttcttcgtct atttccccga cgttttcctc ctgcaaccac
    49141 cgcaatcttt ctgctaataa caccacgcac tcgaaggata aatgggcagt gttcatggcg
    49201 attgtgcaca gcgaccagcg taattcatca atctcttcca tcgagcaata atttaattgc
    49261 tgaatgaatc gctgccaggc ggagggcaga aaacgttcgc tatcagaggc aagtaacgcg
    49321 cgaaaggcga gcagaatttc atgttctgct acctcatatt cttctggttg ccaaacagat
    49381 ttagcaggcg actgcgcaat ccgtagcgcc tcctcatacg cctgacgaag ttgtttaaaa
    49441 ccctgcggat cggtttctgg atgaaaggac ggtaacagcg ccagataagc gcggcggata
    49501 atatcgacgt cagtcgtttc ctctatatcg aggatcttcc agcaattttt cataagtcgc
    49561 tcatatcctt aagcgtttcc gggcatgtgg ggtagtcagc cagtggttgc caggacaagg
    49621 tagggtaata cgcatcatca ctggcaaata attgataaaa catatcattg ccggaatcgt
    49681 cgtattcttt acgcgctaag aattcgcgta ataaaggcag atgatcatct tcgggagact
    49741 gccaatattg ccgaccaaag gtataaccgg caaagtattg ccgccagtta ctataataac
    49801 gtagagcccg tagatgaata cgcgactgga tccatagact ttcttcttca ctcaaccagt
    49861 tattaagtac gcccattcga cttaaaaaac ccatccggac ataatcccat gctttaattc
    49921 ctccctcacc acagcattcg gctgtgctgg cgacaaattg cgcataagcc tgaccttgct
    49981 catttaattc agcaagatag tcacgccatt cacatggaga ataacgaaac cagcggtgat
    50041 aaaaccctgc caaccgagcg gcatgaccgt tctcgatcat tcggtgaatt atgcgcagca
    50101 ggtcatcacg gtcatcaatt ccccatgagg aagaaaggtc aaaaaagcat gtacctaaat
    50161 acattttggg ggatgaatgc gaaggtaaac agcattcatt gcgtatttcc atcggagtgg
    50221 aaagtgcaaa caatattttt tgcgacccca tgtccatata atttatcctt aaacattcat
    50281 ttaatgaatg taaactacat ttcgaggctc agttatttat acttttctga cgcaacataa
    50341 tacgcataaa ataaggctta cgcaaaaatg gaacacataa aaatataata tattttatct
    50401 acaaccataa ggaaatgata atgattttta cgtcaagttg ctgcgataat ttatcaatag
    50461 atgagattat cgaacgtgct gaaaaaggcg attgcgaggc tcaatatatt gttgggtttt
    50521 attataatcg cgatagcgca attgactctc cggacgacga aaaggccttt tactggctga
    50581 agctggccgc tgagcaaggt cattgtgaag cacagtattc cttagggcaa aagtataccg
    50641 aggataaaag ccgtcataaa gataatgagc aagccatctt ctggctgaaa aaagctgccc
    50701 tacaaggcca tactttcgct tccaacgccc ttggctggat actggatcgt ggagaagacc
    50761 ccaactataa agaagcggtt acctggtatc agatagccgc ggagagcgga atgtcttatg
    50821 cgcaaaataa tcttggatgg atgtacagaa atggcaacgg agtcgcacaa gactatacgc
    50881 tggcattttt ctggtacaaa caagctgcat tacaaggcca tagttacgca caagacaatc
    50941 tggccgatct ttataaagac ggagaaggcg ttgctcaaaa caagacactc gccgcattct
    51001 ggtatttgaa aagcgcacag cagggtaatc ggcacgccca gtttcaaatt gcatgggatt
    51061 ataacgctgg cgaaggggtg gaccaggact ataagcaagc gatgtactgg tatctgaagg
    51121 ctgccgctca ggaaagcgtc ggcgcttacg tcaacatcgg ttatatgtat aaacacggac
    51181 aaggcgttga gaaagattat caggctgcct ttgaatggtt tacgaaagcc gctgaatgca
    51241 atgacgccac tgcctggtat aacctggcca ttatgtatca ctacggagaa ggaagacctg
    51301 tcgatctccg acaggctctc gacctgtatc gtaaagttca gtcatccgga accagagatg
    51361 tcagtcagga aattcgtgag actgaagctt tactgtagaa ataaaaacaa gggccgggga
    51421 atttcattct gcccatattc tctttattgc tgtgcgaaat cagactatta cggctcaaac
    51481 gggcgtctct ggaactggtc ataaccacat ttcgggcata gcgtcagcac ttccggtgtg
    51541 tagatcggga gatggaagtg acatttctcg cagaccagat ttcccagccc gaccacttct
    51601 ccgctgtgat aaaccccatg atgattgagg tcctggaaaa tttcgcgcca ttcaagctgc
    51661 gttttatcgg tgatgtctgc cagctcctgc cacaagcttt ctttaatcac ccgcataaag
    51721 acgctgtcag attcttcttt caggctctct tcatagctca tggcgaactc ttccaggtca
    51781 cgtctgacag ctcgcgtcag ctcatcgatc tcggttcgcg ttaactcccc tgtttttatt
    51841 acgcgctcgc gcgcctgttc caccagtgcg tcgatatcac gttcgccatt gcgcaggcgt
    51901 tcgttcagtg acgcgaccag ttcacggtaa tattgagcaa ccttgttcat cgtttcatct
    51961 cccgggtcgt taactgacta taataataga cgttctttgt tccttgcttt gctaatacgc
    52021 ccacaaactc tgtaaattaa tgtacgaata atccgggcat catttcaacg tcgagtgcgc
    52081 gaaaggctgt tttgacgcgg acgtttgggc tatgctatgc ggatctgaaa aaccacctca
    52141 acgctacatt tgtagccgta ttgaaaacag gaccactggc tgccatgcaa gagcaatacc
    52201 gcccggaaga gatagaatcc aaagtacagc ttcattggga tgagaagcgc acatttgaag
    52261 taaccgaaga cgagagcaaa gagaagtatt actgcctgtc tatgcttccc tatccttctg
    52321 gtcgactaca catgggccac gtacgtaact acaccatcgg tgacgtgatc gcccgttacc
    52381 agcgcatgct gggcaaaaac gttctgcagc cgatcggctg ggacgcgttt ggtctgcctg
    52441 cggaaggcgc ggcggtgaaa aacaacaccg ctccggcacc gtggacgtac gacaacatcg
    52501 cgtatatgaa aaaccagctc aaaatgctgg gctttggtta tgactggagc cgcgagctgg
    52561 caacctgtac gccggaatac taccgttggg aacagaaatt cttcaccgag ctgtataaaa
    52621 aaggcctggt atataaaaag acttctgcgg tcaactggtg cccgaacgac cagaccgtac
    52681 tggcgaacga acaagttatc gacggctgct gctggcgctg cgataccaaa gttgaacgta
    52741 aagagatccc gcagtggttt atcaaaatca ctgcttacgc tgacgagctg ctcaacgatc
    52801 tggataaact ggatcactgg ccagacaccg ttaaaaccat gcagcgtaac tggatcggtc
    52861 gttccgaagg cgtggagatc accttcaacg ttaacgacta tgacaacacg ctgaccgttt
    52921 acactacccg cccggacacc tttatgggtt gtacctacct ggcggtagct gcgggtcatc
    52981 cgctggcgca gaaagcggcg gaaaataatc ctgaactggc ggcctttatt gacgaatgcc
    53041 gtaacaccaa agttgccgaa gctgaaatgg cgacgatgga gaaaaaaggc gtcgatactg
    53101 gctttaaagc tgttcaccca ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg
    53161 tattgatgga gtacggcacg ggcgcagtta tggcggttcc gggtcacgac cagcgcgact
    53221 acgagtttgc ctctaaatac ggcctgaaca tcaaaccggt tatcctggcg gctgacggct
    53281 ctgagccaga tctttctcag caagccctga ctgaaaaagg cgtgctgttc aactctggcg
    53341 agttcaacgg tcttgaccat gaagcggcct tcaacgccat cgccgataaa ctgactgcga
    53401 tgggcgttgg cgagcgtaaa gtgaactacc gcctgcgcga ctggggtgtt tctcgccagc
    53461 gttactgggg cgcgccgatt ccgatggtga cgctggaaga cggtaccgta atgccaaccc
    53521 cggacgacca gctgccggtg atcctgccgg aagatgtggt gatggacggc attaccagcc
    53581 cgattaaagc agatccggag tgggcaaaaa ctaccgttaa cggtatgcca gcgctgcgtg
    53641 aaaccgacac tttcgacacc tttatggagt cctcctggta ctatgcgcgc tacacttgcc
    53701 cagagtacaa agaaggtatg ctggattccg aagcggctaa ctactggctg ccggtggata
    53761 tctacattgg tggtatcgaa cacgccatta tgcacctgct ctacttccgc ttcttccaca
    53821 aactgatgcg tgatgcaggc atggtgaact ctgacgaacc agcgaaacag ttgctgtgtc
    53881 agggtatggt gctggcagat gccttctact atgttggcga aaacggcgaa cgtaactggg
    53941 tttccccggt tgatgctatc gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag
    54001 atgcggcagg ccatgaactg gtttataccg gcatgagcaa aatgtccaag tcgaagaaca
    54061 acggtatcga cccgcaggtg atggttgaac gttacggcgc ggacaccgtt cgtctgttta
    54121 tgatgtttgc ttctccggct gatatgactc tcgaatggca ggaatccggt gtggaagggg
    54181 ctaaccgctt cctgaaacgt gtctggaaac tggtgtacga gcacacagca aaaggtgatg
    54241 ttgcggcact gaacgttgat gcgctgactg aagatcagaa agcgctgcgt cgcgatgtgc
    54301 ataaaactat cgctaaagtg accgatgata tcggccgtcg tcagaccttc aacaccgcaa
    54361 ttgcggcgat tatggagctg atgaacaaac tggcgaaagc accgaccgat ggcgagcagg
    54421 atcgcgctct gatgcaggaa gcactgcagg ccgttgtccg tatgctcaac ccgttcaccc
    54481 cgcacatctg cttcacgctg tggcaggaac tcaaaggcga aggcgatatc gacaacgcgc
    54541 cgtggccggt tgctgacgaa aaagcgatgg tggaagactc cacgctggtc gtggtgcagg
    54601 ttaacggtaa agtccgtgcc aaaatcaccg tcccggtgga cgcaacggaa gaacaggttc
    54661 gcgaacgtgc tggccaggaa catctggtag caaaatatct tgatggcgtt actgtacgta
    54721 aagtgattta cgtaccaggt aaactcctca atctggtcgt tggctaagcg cgggaggaag
    54781 cgtgcgatat ctggcaacac tgttgttatc tctggcggtg ttaatcaccg ccgggtgtgg
    54841 ctggcatctg cgtgacacca cgcaggttcc ttccactatg aaggtcatga tcctggactc
    54901 aggcgatccg aacgggccat taagccgtgc ggtgcgtaac cagttacgtc tgaatggtgt
    54961 cgagttgctt gataaagaaa ccacgcgtaa ggacgttcca tccctgcgtt tgggtaaagt
    55021 gagcatcgcg aaagataccg catcggtatt ccgtaacggt caaacagcag agtatcagat
    55081 gatcatgacg gtgaatgcga ccgtgttgat ccccggccgt gatatctacc cgattagcgc
    55141 caaagtcttc cgttcattct tcgataaccc gcaaatggcg ttagcgaaag ataacgaaca
    55201 agacatgatc gtaaaagaga tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc
    55261 aagcatccgt gctgcggata ttcgttccga cgaagaacag acgtcgacca caacggatac
    55321 tccggcaacg cctgcacgcg tctccaccat gctgggtaac tgatgattcg gttgtacccg
    55381 gaacaactcc gcgcgcagct caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac
    55441 gatcctctgt tattgcagga aagccaggac gctgttcgcc aggtagctgc ggcacaagga
    55501 ttcgaagaac accacacttt ttccattgat cccaacactg actggaatgc gatcttttcg
    55561 ttatgccagg ctatgagtct gtttgccagt cgacaaacgc tattgctgtt gttaccagaa
    55621 aacggaccga atgcggcgat caatgaacaa cttctcacac tcaccggact tctgcatgac
    55681 gacctgctat tgatcgtccg cggtaataaa ttaagcaaag cgcaagaaaa tgccgcctgg
    55741 tttactgctc ttgcgaatcg cagcgtgcag gtgacctgtc agacaccgga gcaggctcag
    55801 cttccccgct gggttgctgt gcgcgcaaaa cagctcaact tagaactgga tgacgcggcg
    55861 aatcaggtgc tctgctactg ttatgaaggt aacctgctgg cgctggctca ggcactggag
    55921 cgtttatcgc tgctctggcc agacggcaaa ctgacattac cgcgcgttga acaggcggtg
    55981 aatgatgccg cgcatttcac cccttttcat tgggttgatg ctttgttgat gggaaaaagt
    56041 aagcgcgcgt tgcatattct tcagcaactg cgtctggaag gcagcgagcc ggttattttg
    56101 ttgcgcacat tacaacgtga actgttgtta ctggttaacc tgaaacgcca gtctgcccat
    56161 acgccactgc gtgcgttgtt tgataagcat cgggtatggc agaaccgccg gggcatgatg
    56221 ggcgaggcgt taaatcgctt aagtcagccg cagttacgtc aggccgtgca actcctgaca
    56281 cgaacggaac tcaccctcaa acaagattac ggtcagtcag tgtgggcaga gctggaaggg
    56341 ttatctcttc tgttgtgcca taaacccctg gcggacgtat ttatcgacgg ttgatatgaa
    56401 atctttacag gctctgtttg gcggcacctt tgatccggtg cactatggtc atctaaaacc
    56461 cgtggaaacg ctggcgaatt tgattggtct gacgcgggtc acaatcatcc ctaataatgt
    56521 tcctccgcat cgtccccagc cggaagcgaa cagcgtgcag cgtaaacaca tgcttgaact
    56581 ggcgattgcc gacaagccat tatttactct tgatgaacgc gagctaaagc gcaatgcccc
    56641 ctcttacact gcgcaaacac tgaaagagtg gcggcaggaa caaggaccgg acgtgccgct
    56701 ggcgtttatt attggtcagg attcattgct gacctttccg acctggtacg aatacgaaac
    56761 gatactcgac aatgcacatt tgatcgtctg tcggcgtcca ggttacccac ttgaaatggc
    56821 gcaaccgcaa taccagcaat ggctggaaga tcatttgaca cataacccgg aagatcttca
    56881 ccttcagcct gccggtaaaa tttatctggc tgaaacgccg tggtttaaca tctcggcgac
    56941 catcatccgc gaacgtttgc aaaacggtga atcgtgtgag gatttattgc cggaaccggt
    57001 attgacttac attaaccaac aaggcttgta tcgctgatac ctgtcgttac ggagggcatg
    57061 atgcgactgt ggttaattcg tcatggcgaa acacaagcga atgtcgatgg tctgtacagc
    57121 ggtcatgcgc ccacacccct gaccgcacgc ggtattgagc aagcgcaaaa tctgcataca
    57181 ctgctagatg atgtttcctt tgacttggtt ttatgcagtg aactggaacg ggcacagcat
    57241 accgcgcgac tggttctcag tgaccgccag ctccccgttc atatcatccc tgaactcaac
    57301 gaaatgtttt ttggcgactg ggagatgcga catcatcgcg acctcatgca agaagatgcc
    57361 gaaaactata gcgcgtggtg caatgactgg cagcatgcta tccccacgaa cggtgaagga
    57421 tttcaggcat tttcgcaacg tgtggaacgc tttatcgcaa ggcttagtga atatcagcac
    57481 tatcagaata ttttaatcgt cagccatcag ggcgtactga gtctgttaat tgctcgttta
    57541 attggcatgc ctgccgagtc tatgtggcat tttcgcgttg accaggggtg ttggagcgcc
    57601 attgatatca accaaaaatt cgcgacgcta cgcgtcctca atagccgtgc catcggggtc
    57661 gagaacgcat gacttttctg tttttttacg ggtaagccgc aacgaccatt gacagccccg
    57721 ggcaggctga tattctccgc agccagactt tttccgccag acacgacttt gtagaaattg
    57781 ttttacaaaa atggcgatgc aatctgcggc gcggggtggg atgatagccc actttcgaaa
    57841 gccgattcgg cgacaattgt cccgaaatcg cctctggttc aggtatactg acagaccatt
    57901 tttatctatt tgattcaccc agggggaaaa cttgcagggt aaagcactcc aggattttgt
    57961 tatcgacaaa attgatgacc tcaaaggtca ggacatcatc gccttagacg ttcaaggcaa
    58021 atccagcatc accgactgca tgatcatctg tacgggtacg tccagccgtc atgttatgtc
    58081 cattgctgac cacgttgtgc aggagtctcg cgcagcgggc ctgttaccgc tcggcgtaga
    58141 aggtgaaaac agcgccgact ggattgtcgt ggatttgggc gatgtgattg tccatgtcat
    58201 gcaggaagag agccgtcgcc tgtatgaact ggaaaaactc tggagttaat gcgtgaagct
    58261 gcaacttgtc gccgtgggaa cgaaaatgcc ggactgggta caaaccggtt ttaccgagta
    58321 cctgcgtcgt tttccgaaag atatgccctt cgagctgatt gaaattccgg ccggcaaacg
    58381 cggcaagaat gcggacatca agcgcatact cgacaaagag ggtgagcaga tgttggcggc
    58441 cgcaggcaaa aaccgcattg tcaccctcga tattccaggc aagccctggg atacgccgca
    58501 gttagccgct gagctggaac gctggaagct ggatggtcgc gacgtaagtc tactgattgg
    58561 cgggcctgaa gggttgtcgc ctgcctgtaa agcggcggct gagcagagct ggtcgctgtc
    58621 ggcgcttacc ctcccccatc cgctggttcg cgtgctggtc gcagagagtc tgtaccgggc
    58681 gtggagcatc accaccaacc atccttatca ccgtgagtga taagggagct ttgagtagaa
    58741 aacgcagcgg atgaaactac agaactcttt tcgcgactat acggcagagt ccgcgctgtt
    58801 tgtgcgccgg gcgctggtcg cctttttggg gattttgctg ctgaccggcg tgcttatcgc
    58861 caacctgtat aatctgcaaa ttgttcgctt taccgactac cagacccgct ctaatgaaaa
    58921 ccgcattaag ctggtgccta tcgcgcccag ccgcggcatt atctacgacc gtaacggtat
    58981 ccctctggcc ctcaaccgca ctatctacca gatagaaatg atgccggaga aagtcgataa
    59041 cgtgcagcaa acgctggacg ctttgcgcag cgtggtagat ctgaccgatg acgatattgc
    59101 tgcattccga aaagagcgcg cacgttcaca ccgtttcacc tctattccgg tgaaaactaa
    59161 cctaaccgaa gtacaagtag ctcgctttgc cgtcaatcag taccgttttc cgggtgtcga
    59221 agttaaaggc tataaacgtc gttactatcc ttacggttcg gcgttgaccc acgtcatcgg
    59281 ctatgtgtcg aaaatcaacg ataaagacgt cgaacgcctg aataatgacg gcaaactggc
    59341 caactatgcg gcaacgcatg atattggtaa gctgggcatt gagcgttact atgaagacgt
    59401 gctgcacggt cagaccggtt atgaagaggt tgaagttaac aaccgtgggc gtgttatccg
    59461 ccagttaaaa gaagtaccac cgcaagccgg acacgatatt tacctgacgc tggatctcaa
    59521 actccagcaa tatattgaaa cgctgctggc gggtagccgc gcagctgtgg ttgttaccga
    59581 accgcgtaca ggtggggtgc tggcgctggt ttccacgcct agttatgacc caaacctgtt
    59641 tgttgacggt atctccagca aagattattc cgccttgctg aacgacccga atacaccgct
    59701 ggtgaaccgc gccacacagg gggtttatcc tcccgcgtct acagttaaac cctatgtggc
    59761 ggtttcggca ttaagcgccg gggtgatcac gcgcaatacg acgctgtttg acccaggctg
    59821 gtggcaacta ccagggtcgg aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg
    59881 tctgaatgtc acaagatcgc tggaagaatc tgcggatacc ttcttctatc aggtggccta
    59941 cgatatgggg atcgatcgcc tctccgaatg gatgggtaaa ttcggttatg gtcattacac
    60001 cggtatcgac ctggcggaag aacgttccgg caacatgcct acccgcgaat ggaaacagaa
    60061 acgctttaaa aaaccgtggt atcagggtga caccattccg gttggtatcg gccagggtta
    60121 ctggacagcg accccaatcc agatgagtaa ggcactgatg atcctgatta atgacggtat
    60181 cgtgaaggtt ccgcatttgc tgatgagcac cgccgaagac ggcaaacagg tgccatgggt
    60241 acaaccgcat gaaccgcccg tcggcgatat tcattccggt tactgggagc tggcgaaaga
    60301 cggtatgtac ggtgttgcta accgccctaa tggtacggcg aataaatact ttgccagcgc
    60361 accgtacaaa attgcggcga aatctggtac cgctcaggtc ttcggtctga aagcgaacga
    60421 aacctataat gcgcacaaaa ttgccgagcg tttacgtgac cacaaactga tgaccgcctt
    60481 tgcgccatac aacaatccgc aagtggctgt cgccatgatt ctggagaacg gcggcgcggg
    60541 tccggcggtt ggtacgctga tgcgccagat cctcgaccac attatgctgg gtgataacaa
    60601 caccgatctg cctgcggaaa atccagcggt tgccgcagcg gaggaccatt aatcatgacg
    60661 gataatccga ataaaaaaac attctgggat aaagtccatc tcgatcccac aatgctgctg
    60721 attttactgg cattgctggt ttacagcgcc ctggttatct ggagcgccag cggtcaggat
    60781 attggcatga tggagcgtaa aatcggccaa atcgcgatgg gtctggtcat catggtggtg
    60841 atggcgcaaa ttcctccacg cgtttatgaa ggctgggccc cctatctcta tatcatctgt
    60901 attattttgc tggtggcggt agatgctttc ggagccatct ctaaaggcgc tcaacgctgg
    60961 ctggacctcg gtattgttcg ttttcagccg tcggaaattg ccaaaatagc cgtaccactg
    61021 atggttgcgc gctttatcaa ccgcgacgtt tgcccgccat cgttgaagaa tactggcatc
    61081 gcgctggtgc tgatatttat gcccacgctg ctggtggctg cacagcctga cctgggaaca
    61141 tcaatcctcg ttgcgctttc cggtctgttt gtactgttcc tctctggcct tagctggcgt
    61201 ctgattggcg tcgcagtagt gctggtagcg gcgttcattc cgattctgtg gttcttcctg
    61261 atgcatgatt accagcgcca gcgcgtaatg atgctcctgg acccggaatc agacccactc
    61321 ggcgcgggct atcacattat tcagtctaaa attgctattg gctccggcgg attacgcggc
    61381 aaaggctggc tgcacggcac tcagtcacag cttgaatttc tccccgaacg ccatactgac
    61441 tttatcttcg cggtactggc ggaagagctg ggattagtgg gcattctgat tctgctcgct
    61501 ctctacattc tgctgatcat gcgcgggctg tggatagccg ccagagcgca aaccaccttt
    61561 ggtcgcgtca tggctggtgg cttaatgctg atattattcg tttatgtctt cgtaaatatt
    61621 ggtatggtaa gcggtattct gccggttgta ggggttccgc tcccactggt cagttatgga
    61681 ggatcggcgc taattgtgct gatggctggg ttcgggattg taatgtcaat ccacacccac
    61741 aggaaaatgt tgtcgaaaag cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat
    61801 ctgcatcgcg gcaggaatgc tcgcggcatg tacaagcgat gatggtcagc aacagacggt
    61861 aagtgtaccg cagcctgcgg tatgtaacgg ccccatagtt gaaattagcg gggcggaccc
    61921 gcgtttcgaa ccactgaacg cgacggcaaa tcaggattac cagcgcgacg gtaaaagcta
    61981 caaaatcgtg caggatccgt ctcgatttag ccaggcggga ctggcggcaa tctatgatgc
    62041 cgaaccaggc agtaacctga cggcctctgg cgaagctttc gatccgacaa agctgacggc
    62101 ggcccatcca acgcttccga tccccagcta cgccagaatc actaacctgg ctaacgggcg
    62161 aatgatcgtg gtgcgcatta atgatcgcgg cccttacggc aacgaccgcg ttatttcgct
    62221 ttctcgcgcg gcagctgacc gtcttaacac gtcaaacaac accaaagttc gtatcgatcc
    62281 gattattgtc gcccaggatg gttcgctttc tggtcctggt atggcttgta ccacagtcgc
    62341 caaacagact tacgccctgc ctgcacctcc cgatttaagc ggtggcgcgg gaacaagttc
    62401 agtgtctggc ccgcagggtg acattcttcc ggtcagtaat tcgacgctaa aaagcgaaga
    62461 tccgaccggc gcgccggtaa ccagtagcgg tttcctcggc gctccaacga ccttagcgcc
    62521 tggtgtactg gaaggcagcg aaccgacgcc tgcgccacag cccgttgtta cagcttcgtc
    62581 gacaacgcct gcaacctcgc ctgcaatggt gacaccgcaa gccgcctcgc aaagcgccag
    62641 cggcaacttt atggtgcaag tcggggccgt aagcgatcag gctcgtgcgc aacagtacca
    62701 acagcaactg ggacagaagt tcggcgtccc cggtcgcgta actcaaaatg gcgcggtctg
    62761 gcggatccag cttggcccat tcgccagcaa agccgaagcc agtaccttgc agcaacgttt
    62821 gcaaaccgaa gcccaattac agtcatttat tactaccgcg cagtagcgta aagcaggcat
    62881 ctgatgtgtc aatttctgta aatgacgtta acaaagtcac gcggaaagtc agatgcctgc
    62941 cggtagtgca tttgctatag tagggcactt ttttaattcc atcacggatg tcgtagttca
    63001 gaccatgaat accatttttt ccgctcgtat catgaagcgc ctggcgctca ccacggctct
    63061 ttgcacagcc tttatctctg ctgcacatgc cgatgacctg aatatcaaaa ctatgatccc
    63121 gggtgtaccg cagatcgatg cggagtccta catcctgatt gactataact ccggcaaagt
    63181 gctcgccgaa cagaacgcag atgtccgccg cgatcctgcc agcctgacca aaatgatgac
    63241 cagttacgtt atcggccagg caatgaaagc cggtaaattt aaagaaactg atttagtcac
    63301 tatcggcaac gacgcatggg ccaccggtaa cccggtgttt aaaggttctt cgctgatgtt
    63361 cctcaaaccg ggcatgcagg ttccggtttc tcagctgatc cgcggtatta acctgcaatc
    63421 gggtaacgat gcttgtgtcg ccatggccga ttttgccgct ggtagccagg acgcttttgt
    63481 tggcttgatg aacagctacg ttaacgcact gggcctgaaa aatacccact tccagacggt
    63541 acatggtctg gatgctgatg gtcagtacag ctccgcgcga gatatggcgc tgatcggcca
    63601 ggcattgatc cgtgacgtac cgaatgaata ctcgatctat aaagaaaaag aatttacgtt
    63661 taacggtatt cgccagctga accgtaacgg cctgttatgg gataacagcc tgaatgtcga
    63721 cggcatcaaa accggacaca ctgacaaagc aggttacaat cttgttgctt ctgcgactga
    63781 aggccagatg cgcttgattt ctgcggtgat gggtggacgt acttttaaag gccgtgaagc
    63841 cgaaagtaaa aaactgctaa cctggggctt ccgtttcttc gaaaccgtta acccactgaa
    63901 agtaggtaaa gagttcgcct ctgaaccggt ttggtttagt gattctgatc gcgcttcgtt
    63961 aggggttgat aaagacgtgt acctgaccat tccgcgtggt cgcatgaaag atctgaaagc
    64021 cagctatgtg ctgaacagca gtgaattgca tgcgccgctg caaaagaatc aggtcgtcgg
    64081 aactatcaac ttccagcttg atggcaaaac gatcgagcaa cgcccgctgg ttgtgttgca
    64141 agaaatcccg gaaggtaact tcttcggcaa aatcattgat tacattaaat taatgttcca
    64201 tcactggttt ggttaaaaat tgaacacttg aaagtgtaat ttccgtcccc atatactaag
    64261 catcagtaaa aaaactcccg ccttctggcg ggagttgcta tttaattacg ttacgccgga
    64321 gctgacatga aaaccaaact taacgaactg cttgaattcc ctactccttt tacttacaaa
    64381 gttatggggc aggcgttacc tgagctggtt gatcaggtgg ttgaagtggt acagcgccat
    64441 gcgccaggtg actacacccc aacggtaaaa ccaagcagca aaggcaacta ccactcggta
    64501 tctatcacta tcaacgccac tcatatcgag caggttgaaa cactgtatga agaactgggc
    64561 aaaatcgata ttgtccgcat ggttctgtaa ctcgcttctc cgttacccgg ctcctggtcg
    64621 ggtaactccc ctctcctcgc tgtgatatac ttttccccca cttttactca ttctccacgg
    64681 agatgccgtt ttgtatcagg ataaaattct tgtccgccag ctcggtcttc agccttacga
    64741 gccaatctcc cgggctatgc atgaattcac cgatacccgc gataatagta cccttgatga
    64801 aatctggctg gtcgagcact atccggtatt cacccaaggt caggcaggaa aagcggagca
    64861 cattttaatg ccgggtgata ttccggtgat ccagagcgat cgcggtgggc aggtgactta
    64921 tcacgggccg ggacaacagg tgatgtatgt gttgcttaac ctgaaacgcc gtaaactcgg
    64981 tgtgcgtgaa ctggtgacct tgcttgagca aacagtggtg aataccctgg ctgaactggg
    65041 tatagaagcg catcctcggg ctgacgcgcc tggtgtctat gtcggggaaa agaaaatttg
    65101 ctcactgggt ttacgaattc gacgcggttg ttcattccac ggtctggcat taaacgtcaa
    65161 tatggatctt tcaccatttt tacgtattaa tccttgtggg tatgccggaa tggaaatggc
    65221 taaaatatca caatggaaac ccgaagcgac gactaataat attgctccac gtttactgga
    65281 aaatatttta gcgctactaa acaatccgga cttcgaatat attaccgctt aattccatac
    65341 atcaatggcc caatttacac tgggtcatta ctcataatac ctgctaatgc tttacatctc
    65401 cttcgcgttt gtatattttc tttatcactt gcagaaatgc ggtgccacgc cttgctccgt
    65461 aactcacccc attgattaac ctattgaaat ggttgattat ggtgagcaaa agcgttccgt
    65521 tccaattaga cataagatta ttgtaatttt ttaaatatta tttttccatg aataaaattg
    65581 gagtaagtgc gtggatagta ataatcaaat tgaaccctgt ttaagcagaa agtcgtcaga
    65641 aggtaaacca caaatattta caaccttacg aaatattgat cttaaccttc tgactatttt
    65701 tgaagctgta tatgtacata aagggatcgt taatgcagcg aaagtgctta atctgacccc
    65761 ctcggcaatc agtcagtcta ttcagaaact gcgcgttata ttccctgacc cattgtttat
    65821 tcgcaaaggc cagggtgtca ctcctaccgc atttgcgatg catctacatg agtatatcag
    65881 tcagggcctt gagtccattc ttggcgcgct ggatatcgaa ggaagctatg ataagcaacg
    65941 aacgataact attgcgacca ctccctcggt cggagccctg gtccttcctg tcatctatcg
    66001 ggcgattaaa actcactatc cgcagctttt actgcgcaac ccgcccatca gcgacgcgga
    66061 aaaccaactc agtcagtttc aaaccgatct catcatcgat aacatgtttt gcaccaatcg
    66121 tacggtgcaa catcatgttc tgttcaccga caatatggtg ttaatttgcc gcaagggaaa
    66181 tccactactc tctttagaag atgacagaga gactatcgac aacgctgcgc atgtacttct
    66241 gttaccggaa gggcaaaatt tcagcggtct gcggcagaga gttcaagaga tgtttccgga
    66301 ccggcaaatc aatttcacca gctacaacat tttgacaatc gctgcactgg ttgccaacag
    66361 tgacatgtta gcgattattc ccagccgttt ttataacctg tttagtcgtt gctggccgct
    66421 ggaaaaactg ccttttccgt ccttaaatga ggagcaaata gatttctcta tccactacaa
    66481 caaattcatc ctgcgtgatc ccatcctgca cggagtaata gatgttatcc gtaatgcatt
    66541 ttgaaaaagt attgttacag ccagatcaca gcgcagaacg ataaaatggc acaatccaac
    66601 aacaatttta cattttcgcg accgctttgg ctgctttcag gtccgtttca atgatatact
    66661 gccagtcgtt aattcaaaaa tagttgataa ttacaacaat ctattgaatt gaaacgcttt
    66721 ccttcgtaat tcgcaactgg aacacgcacg ctatgagtaa acccattgtg atggaacgcg
    66781 gtgttaaata ccgcgatgcc gataagatgg cccttatccc ggttaaaaac gtggcaacag
    66841 agcgcgaagc cctgctgcgc aagccggaat ggatgaaaat caagcttcca gcggactcta
    66901 cacgtatcca gggcatcaaa gccgcaatgc gcaaaaatgg cctgcattct gtctgcgagg
    66961 aagcctcctg ccctaacctg gcggaatgct tcaaccacgg cacagcaacg tttatgatcc
    67021 tcggcgctat ttgtacccgc cgttgtccgt tctgtgatgt tgcccacggt cgcccggtag
    67081 ctcctgatgc caatgaacca gtgaaactgg cgcagaccat tgccgatatg gcgctgcgtt
    67141 atgtggttat cacctccgtt gaccgtgatg acctgcgcga tggcggtgcc caacactttg
    67201 cggattgtat tactgccatt cgggaaaaaa gcccgcaaat caaaattgaa actctggtgc
    67261 cggatttccg cggtcgaatg gatcgtgctc tggatattct gactgcaacg cccccagatg
    67321 tgttcaacca taacctggaa aacgtaccgc gtatttaccg gcaggtacgg cctggcgcag
    67381 attacaactg gtcgctgaag ctgctggaac gctttaaaga agcgcatccg gaaatcccga
    67441 ccaagtctgg tctgatggtg ggtctgggtg aaaccaatga agaaattatt gaggtaatgc
    67501 gcgacctgcg ccgtcatggt gtgacgatgt taacgctggg gcaatatttg cagccaagcc
    67561 gccatcacct gccggttcaa cgttacgtta gcccggatga gttcgacgaa atgaaagccg
    67621 aagcgctggc gatgggcttt acccatgctg catgcggtcc gtttgtccgc tcttcttacc
    67681 acgccgattt gcaggcgaaa ggaatggaag ttaagtaatc ctgtaataat tacttacatc
    67741 tgtctgataa aaaacccgct ttttgaagcg ggttttttgt atcgaacaag atattgaggg
    67801 agcgtcctgc tcgccacgtc actctttatg agagagcttt tcagcctgaa gatcaacgtc
    67861 tgcgcctttt ttcgccgcag catcgtcatc attcatcgcc ttcttgaacc ctttaatggc
    67921 cgctccaagg tctccgccca gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag
    67981 cgccgcaact accagcagtt tggtaatact aatctcaccc atagatacct tcttgacata
    68041 taaccggcaa cagaacgcgc cttaaaaacc agagaatatt aacggtcatt cgacgcgcgc
    68101 acacaatagc aatctgtaac aaggtgaatc aagcgccgag taaaaaaaca tcataataat
    68161 tgcggcggcg caaagcgacg gttgtttaag atgggcagtt gcgcgcgcac ctgacgcaca
    68221 cgttcgggcg tcacttccgc cataatgagt gcaggcattt ctgacgctgc cgcaatggtg
    68281 acgccaaagg gatctataat ccggctttgc ccgatatttt tgttcccgca ctcccccgcc
    68341 gccaccatat aacaggtggt atccagcgca cgagcggcaa gcaacgttga ccagtgatgc
    68401 tctttgagcg gcccacgaac ccaggcggca ggaagtacca ggatttcagc tccctgtaaa
    68461 gcctgtgcca gcgccagctc tggaaagcgt aagtcataac aggtcatcag accgaccttc
    68521 atcccttcca cctccagtaa cggagctatt tcattaccag catcaacatg gcgtgattcc
    68581 tgaatggcaa atgcatcata gagatgcagt ttggcataac gggcgacgat gtttcctgcc
    68641 tgaagtgcca ccagcatatt ccatgcgcgc cccggcgttg aaggaagatg aatcgtcaga
    68701 attgtcgtca tcatgttacg tttactttct cgccgtaaac gtccgaggaa ttcgccttcc
    68761 agcagctgtg ctgatttaac cgatagatct gcatcatgat catcgcgcgc cagcaatgct
    68821 tccggcagga caaacagcga tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa
    68881 atctcagcgt tcttttccca cacagatgta acagcaaact gtcctgcagc aaccagcatt
    68941 gccacctcct gtcagagtcg taaaattcgc caacgacgat tattcggcgt tacacttatc
    69001 actcatacaa atcaaatagc aggattttgc agtgttacaa cttcttttag cagtttttat
    69061 tggcggtggt acgggaagcg tagcgagatg gctgttaagt atgcgattta acccgctgca
    69121 tcaggcgatt ccgttgggga cgctggcagc aaacctgatt ggggcattca tcataggaat
    69181 gggattcgcc tggttcagca ggatgacgaa cattgatcca gtgtggaaag tattaatcac
    69241 caccggattt tgtggcggtc taacaacctt ctcaacattt tcggcagaag tggtgttttt
    69301 gttacaagag ggccgctttg gctgggcatt actgaacgtt ttcgtcaacc ttctggggtc
    69361 ttttgccatg accgcactgg cattctggct gttttcggcc tcaaccgtac actaaagacc
    69421 aaaaaaaacc cgctgattaa gcgggttttg aattcttgct gacgtatctt acagagcgat
    69481 tacgtttgca gcagaagggc ctttggcacc gttagtgatt tcgaactcta cgcgctgacc
    69541 ttcagcaaga gttttaaaac cattagtctg gattgcagag aagtgtacga acacgtcttt
    69601 gctgccgtct tccggagtaa tgaaaccgaa tcctttggac tcattaaacc acttaacgtt
    69661 acctttaatc ttagacatca aaattacctt tacatgaaaa atagacacaa atgctgtgtc
    69721 ggttatcagt acaccaattg tggtacgctt tgtccagtgc aactttacta aaaagtgata
    69781 aaagtcgcgt tatttttgat gttgcttatt ttagttattg attttaaaga agttactaaa
    69841 acttcatttg tctcaaaact gaaagcgcat ccaggcaaag tacacattgc cattgttgta
    69901 ggtacccgga atgtaggtca tctgaaaagt cactgggcca taacccacgg aggccaatgg
    69961 cagtagaacc gggagaggga tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa
    70021 tcccagacct aaatgaaaat tttcatccgc cagcggtcgc caggtacttt cccatccgta
    70081 tccggcaatc ggttcccatt tgttccacga gtccttaaat gccatggcat acaggccatg
    70141 ccagtttcct ttttcatccc aacgcgacag gccaaaaccg ccaccccacg gtcgctcgtt
    70201 atagcgatcg gttttttctt tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat
    70261 atataaatca taatgttcag gctgttgcca ggtttgtgca atattttctc taaacgttgt
    70321 catcctctca tctgcgttag caaaaacttt accaacgctg attaactgaa taaaaacaaa
    70381 ggaaaagata gcgacatatt tactcacgtt catttgtgac cataaaacat ttatcaaaaa
    70441 tctactacta gcatagcaaa gctacaatta acataacctt aatagaccca acataaagaa
    70501 taatctgaat agctgctcta tttatttccg cttgttcaag ttaaatattt ccgcgcatcg
    70561 attaaagatg atgcgtcgtt ttctgcgatg ggaatagtca aaaaagaaaa accaagtctt
    70621 ttttgatgac aaaatgcaat caaggaaaaa ttattttatt tttttaacga gatacaacaa
    70681 tcatcttaac gaagtatata atatatatcg tcaacccatg ctcatattag attatttttt
    70741 gagcgcacag ccgcagctaa caccattgca attaacaaat ttgcatcaat ccaccatcaa
    70801 tttacacaca ttattatgtg ataattgcca accgctaaat atgcgttttg ttatctatgt
    70861 ataaaaacag caacttcaat gtcttaatgg cagtttttct tgattttaat cagcattcat
    70921 cgccaattta ttgggcatat tttttcctta agctttagga agtttttatt atttactttg
    70981 ggcctggaga caggaaaaat tatgctgaca ttcattgaac tccttattgg ggttgtggtt
    71041 attgtgggtg tggctcgcta catcattaaa gggtattccg ccactggtgt gttatttgtc
    71101 ggtggcctgt tattgctgat tatcagtgcc attatggggc acaaagtgtt accgtccagc
    71161 caggcttcaa caggctacag cgccacggat atcgttgaat acgttaaaat attactaatg
    71221 agccgcggcg gcgacctcgg catgatgatt atgatgctgt gtggctttgc cgcttacatg
    71281 acccatatcg gcgcgaatga tatggtggtc aagctggcgt caaaaccatt gcagtatatt
    71341 aactcccctt acctgctgat gattgccgcc tattttgttg cttgtctgat gtcactggcc
    71401 gtctcttccg caaccggtct gggggttttg ctgatggcaa ccctgtttcc ggtgatggta
    71461 aacgttggta tcagtcgtgg cgcagcagct gccatttgtg cctccccggc ggcgattatt
    71521 ctcgcaccga cttcagggga tgtggtgctg gcggcgcagg cttccgaaat gtcgctgatt
    71581 gacttcgcct tcaaaacgac gctgcctatc tcaattgctg caattatcgg catggcgatc
    71641 gcccacttct tctggcaacg ttatctggat aaaaaagagc acatctctca tgaaatgtta
    71701 gatgtcagtg aaatcaccac cactgccccc gcgttttatg ccattttgcc gttcacgccg
    71761 atcatcggtg tactgatttt tgacggtaaa tggggtccgc aattacacat catcactatt
    71821 ctggtgattt gtatgctgat tgcctccatt ctggagttcc tccgcagctt taatacccag
    71881 aaagttttct ctggtctgga agtggcttat cgcgggatgg cagatgcgtt tgctaacgtg
    71941 gtgatgctgc tggttgccgc tggggtattc gctcaggggc ttagcaccat cggctttatt
    72001 caaagtctga tttctatcgc tacctcgttt ggttcggcga gtatcatcct gatgctggta
    72061 ttggtgattc tgaccatgct ggcggcagtc acgaccggtt caggcaatgc gccgttttat
    72121 gcgtttgttg agatgatccc gaaactggcg cactcttccg gcattaaccc ggcgtatttg
    72181 actatcccga tgctgcaggc gtcaaacctg ggtcgtaccc tatcacccgt ttctggcgta
    72241 gtcgttgcgg ttgccgggat ggcgaaaatc tcaccatttg aagtcgtaaa acgcacctcg
    72301 gtgccggtgc ttgttgggct agtgattgtt atcgttgcta cagagctgat ggtgccagga
    72361 acggcagcag cggtcacagg caagtaaata gtaatgccgg aggcaagttt cctccggctt
    72421 tatcatgtca cccactgtgg tatatgcgtt gtggtctgcc aactttgccg tgaacaattt
    72481 cagcaataat cagatggcgg ctggcgcaat attcaagata acgcctggca gtggtgcggc
    72541 tgatggtcag tgcctgcgcc accgtttctg ccgtatgttg cacaccaggc tctttaaaca
    72601 gttttcgcac cgcgtttagc gtcaaggggt caatgccggt cggcagttcg tctttgggtt
    72661 caccgcgagc ataggcatta aacatctcat caatttgctt ctggctggcg ctatcaatac
    72721 tttccagcat atgtttacgc tggcggaaac gggttagcgt ttgccccagt gtattgattc
    72781 acttgaagta cgaaaaaacc gggagaacat tggattattc gggatctgat gggattagat
    72841 ttggtggggc ttgcaagcct gtagtacaaa ttttaggcgt taaccaatga aacgcgaaag
    72901 atagtaaaaa attgcttttg tttcattgaa aaaacgaaaa acaaaaacac tataaatcat
    72961 ttcaatcata gcttcaaaaa acgttcaaaa ccgataacaa ccaggctgtc atcaaatgac
    73021 tcatatcaca aatcagctca tgccgtttag gtatgttaca tgtgtgatta tgtgaggtga
    73081 agtatgtttt agctggctca tggttgttat acggcttttt tcgcatcttg tggttccaac
    73141 aaaggagcta caggctcttc ctgtttatga acaccataca ttgacaaaat ctcttggcga
    73201 gtttctggag gtaatttatg cagcgccaaa gctgtttgta gtgtttggcg atcaataccc
    73261 agcgttgcca atagggtgtc aacggagatg gaggggcgtt cttgcattaa tccggaaagc
    73321 ccatatttag ccatgtttag ctctatctct cggacttcat tttctcctag tttattgatt
    73381 aacagggaga acattttcaa tgtatcagca ttcatttctt cgatagcaat tttttgcata
    73441 gccggtattg ccgggatata ttcaacccca ggcccgcgac gcccttcttg tgaacgcttc
    73501 ggccatcctt ctttctttgc tcgtttatgc accccctgta ttgtcccagg cattccatcc
    73561 agttcagtaa tttgtttggg tgtcatccac tggtttatct catttgcgct tttcataaac
    73621 taagtttacc agatttatct atataaaaat caatggatta caaaataaac cacattaact
    73681 gatataaacc ggtttggttt atttggtaat gtataaaaca gataatcaag acgaattatt
    73741 tgttttggta accctttatc atacgcaata tggttggtgt gcgaaagatg agtaacgatt
    73801 ggcatcgggc cgacgtgatt gcggccctca aaaaaacagg aacccctctg gcaaaattgt
    73861 ccagacgttc aggttattca accgggacgc ttgcaaatgc attagtcagg ccatggccta
    73921 aaggtgaacg cattattgct gacgccttaa acatgaaacc tgaggaaatt tggcctagta
    73981 gatatctaag ggatcacaat gagagagtgg atatcagcaa ttgaatgcat gggtttatct
    74041 ggtttacctg gttcattaat gggggttcac aaaagagctc agagagaagg atggctcaag
    74101 agaagaagag aaggaattag aggggttgct tacgaatacc atatatcttc tcttcctaac
    74161 attgctcgag ctgaagtttt gctccaacaa ggaaaattta aaaccagtca gggatgtttt
    74221 gaaatcgccc gccccacgct ggaagctcat gattatgatc gtgaggcact atggagcaaa
    74281 tgggataaag ccagcgattc ccagcgcagc ctggctgaaa aatggttgcc ctcaattcag
    74341 gccacagacg aaatgctgaa ccaggggatt tcaacgaaaa cggcttttgc gaccgttgca
    74401 gggcattacc aggtcagcgc atccactttg cgggacaagt attaccaggt acagaagttt
    74461 gcgaagcctg actgggcggc tgcacttgtt gatggacgtg gagcatcccg tcgcaatgtt
    74521 cacaaaagtg aatttgacga ggatgcctgg cagtttctga ttgcagatta tctgcgaccg
    74581 gaaaaacccg ccttccgcaa atgttatgag cgtctggaac tggcagcccg cgagcatggc
    74641 tggagtattc cctcccgtgc cacggccttt cgccggattc agcaactgga cgaggcaatg
    74701 gttgttgcct gtcgtgaagg tgaacatgca ctgatgcatc tgataccggc acagcagcga
    74761 actgtggaac acctggacgc catgcagtgg atcaacggcg acggttatct gcataacgtc
    74821 tttgtacgct ggtttaacgg tgatgtgatc cgtccgaaaa catggttctg gcaggatgtg
    74881 aaaacccgaa aaattctggg ctggcgctgc gatgtgagcg agaacattga ttcaattcgc
    74941 ctctcgttca tggatgttgt gactcgctac agtatcccgg aggattttca catcaccatt
    75001 gataacaccc gtggtgctgc gaataaatgg ctgacgggag gcgcgcccaa tcgctaccgc
    75061 tttaaggtaa aagaggacga tccaaaagga ctgtttttac tgatgggtgc gaaaatgcac
    75121 tggacaagcg ttgttgccgg taaaggctgg ggccaggcaa aacctgttga acgtgctttc
    75181 ggtgttggtg ggcttgagga atacgttgat aagcatccgg cactggctgg cgcatatacg
    75241 gggccaaatc cgcaggcaaa acctgataac tatggcgacc acgctgttga tgcagagctg
    75301 tttctgaaaa cctttgccga aggtgtggcg atgttcaatg ccagaacagg ccgtgaaaca
    75361 gaaatgtgcg ggggcaaact ctcgtttgat gatgttttcg agcgtgaata cgccagaacg
    75421 attgtgcgta agccaaccga agaacaaaaa cggatgctgt tactgcctgc cgaggcggtg
    75481 aacgtttcac gcaaaggcga gtttacgctt aaagttggcg gctcccttaa aggcgcgaaa
    75541 aacgtttatt acaacatggc attaatgaat gccggagtga aaaaagttgt ggtcagattt
    75601 gatccacagc agttacacag cacggtttat tgag
//
LOCUS       DAJTUI010000004        73323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTUI010000004 DAJTUI010000000
VERSION     DAJTUI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 73323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.004"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..596)
                     /locus_tag="OO464_000303"
     CDS             complement(<1..596)
                     /locus_tag="OO464_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR6225580.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(667..2781)
                     /gene="fusA"
                     /locus_tag="OO464_000304"
     CDS             complement(667..2781)
                     /gene="fusA"
                     /locus_tag="OO464_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6225581.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="OO464_000305"
     CDS             complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="OO464_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6225582.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="OO464_000306"
     CDS             complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="OO464_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6225583.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="OO464_000307"
     CDS             complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="OO464_000307"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6225584.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="OO464_000308"
     CDS             complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="OO464_000308"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6225585.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="OO464_000309"
     CDS             complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="OO464_000309"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6225586.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4985..5707)
                     /locus_tag="OO464_000310"
     CDS             complement(4985..5707)
                     /locus_tag="OO464_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6225587.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="OO464_000311"
     CDS             complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="OO464_000311"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6225588.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6907..7125
                     /locus_tag="OO464_000312"
     CDS             6907..7125
                     /locus_tag="OO464_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6225589.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="OO464_000313"
     CDS             complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="OO464_000313"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6225590.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7859..8059)
                     /locus_tag="OO464_000314"
     CDS             complement(7859..8059)
                     /locus_tag="OO464_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6225591.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(8069..9806)
                     /gene="kefB"
                     /locus_tag="OO464_000315"
                     /pseudo
     CDS             complement(8069..9806)
                     /gene="kefB"
                     /locus_tag="OO464_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9806..10357)
                     /gene="kefG"
                     /locus_tag="OO464_000316"
     CDS             complement(9806..10357)
                     /gene="kefG"
                     /locus_tag="OO464_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6225592.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10488..12401
                     /locus_tag="OO464_000317"
     CDS             10488..12401
                     /locus_tag="OO464_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6225593.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12401..13423
                     /locus_tag="OO464_000318"
     CDS             12401..13423
                     /locus_tag="OO464_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6225594.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13417..13635
                     /locus_tag="OO464_000319"
     CDS             13417..13635
                     /locus_tag="OO464_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6225595.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13689..14558
                     /locus_tag="OO464_000320"
     CDS             13689..14558
                     /locus_tag="OO464_000320"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6225596.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14613..15017)
                     /locus_tag="OO464_000321"
     CDS             complement(14613..15017)
                     /locus_tag="OO464_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6225597.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15319..15951
                     /gene="crp"
                     /locus_tag="OO464_000322"
     CDS             15319..15951
                     /gene="crp"
                     /locus_tag="OO464_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6225598.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16002..18092
                     /locus_tag="OO464_000323"
     CDS             16002..18092
                     /locus_tag="OO464_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6225599.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18159..19379)
                     /gene="argD/dapC"
                     /locus_tag="OO464_000324"
     CDS             complement(18159..19379)
                     /gene="argD/dapC"
                     /locus_tag="OO464_000324"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6225600.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19465..20028)
                     /gene="pabA"
                     /locus_tag="OO464_000325"
     CDS             complement(19465..20028)
                     /gene="pabA"
                     /locus_tag="OO464_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6225601.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(20060..20662)
                     /locus_tag="OO464_000326"
     CDS             complement(20060..20662)
                     /locus_tag="OO464_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR6225602.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20652..20819)
                     /locus_tag="OO464_000327"
     CDS             complement(20652..20819)
                     /locus_tag="OO464_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6225603.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20924..21505)
                     /gene="ppiA"
                     /locus_tag="OO464_000328"
                     /gene_synonym="rotA"
     CDS             complement(20924..21505)
                     /gene="ppiA"
                     /locus_tag="OO464_000328"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6225604.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21776..22957
                     /gene="tsgA"
                     /locus_tag="OO464_000329"
     CDS             21776..22957
                     /gene="tsgA"
                     /locus_tag="OO464_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6225605.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23219..25762
                     /gene="nirB"
                     /locus_tag="OO464_000330"
     CDS             23219..25762
                     /gene="nirB"
                     /locus_tag="OO464_000330"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6225606.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25759..26085
                     /gene="nirD"
                     /locus_tag="OO464_000331"
     CDS             25759..26085
                     /gene="nirD"
                     /locus_tag="OO464_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6225607.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26211..27017
                     /gene="nirC"
                     /locus_tag="OO464_000332"
     CDS             26211..27017
                     /gene="nirC"
                     /locus_tag="OO464_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6225608.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27036..28409
                     /gene="cobA"
                     /locus_tag="OO464_000333"
     CDS             27036..28409
                     /gene="cobA"
                     /locus_tag="OO464_000333"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6225609.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28656..28823
                     /locus_tag="OO464_000334"
     CDS             28656..28823
                     /locus_tag="OO464_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR6225610.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            29118..30455
                     /gene="frlA"
                     /locus_tag="OO464_000335"
                     /pseudo
     CDS             29118..30455
                     /gene="frlA"
                     /locus_tag="OO464_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30476..31498
                     /gene="frlB"
                     /locus_tag="OO464_000336"
     CDS             30476..31498
                     /gene="frlB"
                     /locus_tag="OO464_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6225611.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31548..32378
                     /gene="frlC"
                     /locus_tag="OO464_000337"
     CDS             31548..32378
                     /gene="frlC"
                     /locus_tag="OO464_000337"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR6225612.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32375..33160
                     /gene="frlD"
                     /locus_tag="OO464_000338"
     CDS             32375..33160
                     /gene="frlD"
                     /locus_tag="OO464_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR6225613.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33260..33991
                     /gene="phoB"
                     /locus_tag="OO464_000339"
     CDS             33260..33991
                     /gene="phoB"
                     /locus_tag="OO464_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6225614.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(34151..35236)
                     /locus_tag="OO464_000340"
     CDS             complement(34151..35236)
                     /locus_tag="OO464_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6225615.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35248..36552)
                     /locus_tag="OO464_000341"
     CDS             complement(35248..36552)
                     /locus_tag="OO464_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6225616.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36564..36917)
                     /locus_tag="OO464_000342"
     CDS             complement(36564..36917)
                     /locus_tag="OO464_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR6225617.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGADAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36928..37806)
                     /locus_tag="OO464_000343"
     CDS             complement(36928..37806)
                     /locus_tag="OO464_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6225618.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37803..39029)
                     /locus_tag="OO464_000344"
     CDS             complement(37803..39029)
                     /locus_tag="OO464_000344"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6225619.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(39030..40193)
                     /locus_tag="OO464_000345"
     CDS             complement(39030..40193)
                     /locus_tag="OO464_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR6225620.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(40277..40639)
                     /locus_tag="OO464_000346"
     CDS             complement(40277..40639)
                     /locus_tag="OO464_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6225621.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40656..41561)
                     /locus_tag="OO464_000347"
     CDS             complement(40656..41561)
                     /locus_tag="OO464_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225622.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41831..42835)
                     /gene="trpS"
                     /locus_tag="OO464_000348"
     CDS             complement(41831..42835)
                     /gene="trpS"
                     /locus_tag="OO464_000348"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6225623.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42828..43586)
                     /gene="gph"
                     /locus_tag="OO464_000349"
     CDS             complement(42828..43586)
                     /gene="gph"
                     /locus_tag="OO464_000349"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6225624.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43579..44256)
                     /gene="rpe"
                     /locus_tag="OO464_000350"
     CDS             complement(43579..44256)
                     /gene="rpe"
                     /locus_tag="OO464_000350"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6225625.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(44274..45110)
                     /gene="dam"
                     /locus_tag="OO464_000351"
     CDS             complement(44274..45110)
                     /gene="dam"
                     /locus_tag="OO464_000351"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6225626.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(45217..46509)
                     /gene="damX"
                     /locus_tag="OO464_000352"
     CDS             complement(45217..46509)
                     /gene="damX"
                     /locus_tag="OO464_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6225627.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46601..47689)
                     /gene="aroB"
                     /locus_tag="OO464_000353"
     CDS             complement(46601..47689)
                     /gene="aroB"
                     /locus_tag="OO464_000353"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6225628.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47746..48267)
                     /gene="aroK"
                     /locus_tag="OO464_000354"
     CDS             complement(47746..48267)
                     /gene="aroK"
                     /locus_tag="OO464_000354"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6225629.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48671..49909)
                     /gene="hofQ"
                     /locus_tag="OO464_000355"
     CDS             complement(48671..49909)
                     /gene="hofQ"
                     /locus_tag="OO464_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6225630.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49821..50227)
                     /gene="hofP"
                     /locus_tag="OO464_000356"
                     /pseudo
     CDS             complement(49821..50227)
                     /gene="hofP"
                     /locus_tag="OO464_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(50217..50657)
                     /gene="hofO"
                     /locus_tag="OO464_000357"
     CDS             complement(50217..50657)
                     /gene="hofO"
                     /locus_tag="OO464_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6225631.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50641..51180)
                     /gene="hofN"
                     /locus_tag="OO464_000358"
     CDS             complement(50641..51180)
                     /gene="hofN"
                     /locus_tag="OO464_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR6225632.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(51180..51959)
                     /gene="hofM"
                     /locus_tag="OO464_000359"
     CDS             complement(51180..51959)
                     /gene="hofM"
                     /locus_tag="OO464_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6225633.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            52079..54631
                     /gene="mrcA"
                     /locus_tag="OO464_000360"
     CDS             52079..54631
                     /gene="mrcA"
                     /locus_tag="OO464_000360"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6225634.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54796..55356)
                     /gene="nudE"
                     /locus_tag="OO464_000361"
     CDS             complement(54796..55356)
                     /gene="nudE"
                     /locus_tag="OO464_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6225635.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55676..57811
                     /gene="igaA"
                     /locus_tag="OO464_000362"
     CDS             55676..57811
                     /gene="igaA"
                     /locus_tag="OO464_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6225636.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57876..58544
                     /gene="yrfG"
                     /locus_tag="OO464_000363"
     CDS             57876..58544
                     /gene="yrfG"
                     /locus_tag="OO464_000363"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6225637.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58555..58956
                     /gene="hslR"
                     /locus_tag="OO464_000364"
     CDS             58555..58956
                     /gene="hslR"
                     /locus_tag="OO464_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6225638.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58981..59850
                     /gene="hslO"
                     /locus_tag="OO464_000365"
     CDS             58981..59850
                     /gene="hslO"
                     /locus_tag="OO464_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6225639.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59994..61745)
                     /locus_tag="OO464_000366"
     CDS             complement(59994..61745)
                     /locus_tag="OO464_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6225640.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            62124..63746
                     /gene="pckA"
                     /locus_tag="OO464_000367"
     CDS             62124..63746
                     /gene="pckA"
                     /locus_tag="OO464_000367"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6225641.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63822..65174)
                     /gene="envZ"
                     /locus_tag="OO464_000368"
     CDS             complement(63822..65174)
                     /gene="envZ"
                     /locus_tag="OO464_000368"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6225642.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(65171..65890)
                     /gene="ompR"
                     /locus_tag="OO464_000369"
     CDS             complement(65171..65890)
                     /gene="ompR"
                     /locus_tag="OO464_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6225643.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            66118..66594
                     /gene="greB"
                     /locus_tag="OO464_000370"
     CDS             66118..66594
                     /gene="greB"
                     /locus_tag="OO464_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6225644.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66691..69012
                     /locus_tag="OO464_000371"
     CDS             66691..69012
                     /locus_tag="OO464_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6225645.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69450..69677
                     /gene="feoA"
                     /locus_tag="OO464_000372"
     CDS             69450..69677
                     /gene="feoA"
                     /locus_tag="OO464_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6225646.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69694..72015
                     /gene="feoB"
                     /locus_tag="OO464_000373"
     CDS             69694..72015
                     /gene="feoB"
                     /locus_tag="OO464_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6225647.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            72015..72251
                     /gene="feoC"
                     /locus_tag="OO464_000374"
     CDS             72015..72251
                     /gene="feoC"
                     /locus_tag="OO464_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6225648.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72454..>73323
                     /gene="rpnA"
                     /locus_tag="OO464_000375"
     CDS             72454..>73323
                     /gene="rpnA"
                     /locus_tag="OO464_000375"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR6225649.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAA"
BASE COUNT        17662 a        19877 c        18552 g        17232 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcgtactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcatat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cacgagcagc accgccgtag
      481 gttttagcca gtacggtggt gattgcagcg gtcagagtag ttttaccgtg gtcaacgtgg
      541 ccgatagtac caacgttaac gtgcggtttt gtacgttcaa atttttcttt agacacggct
      601 atattcctta ctatagtgct ctccccttca ggagagagca cgggactttg gtattaaccc
      661 ttaggcttat ttaccacggg cttcaattac ggcctgagca acgttactcg gcgcttcatc
      721 atacttcagg aattccatag tgtatgatgc acgacctttg gtcagagaac gcagctgagt
      781 tgcgtatccg aacatttcag acagcggtac ttcagcgtgg atcttaacgc cagtaacttc
      841 agattcctga cctttgagca taccacgacg acggctcaag tcaccgataa cgtcaccggt
      901 gttctcttcc ggagtttcta cttcaacctt catgatcggc tcaagcagaa ctggtttcgc
      961 tttcttaaag ccttctttaa aggcgataga agcagccagt ttaaacgcca gttcagagga
     1021 gtcaacgtca tggtaagaac cgaagtgcag acgaataccc atgtctacta ccgggtagcc
     1081 tgccagcgga cctgctttca gctgttcctg gataccttta tcaacggccg ggatgtattc
     1141 gccagggatt acaccacctt taatgtcgtt gatgaactcg tagcctttcg ggtttgaacc
     1201 cggctccagc gggtacatgt cgataacaac atgaccatac tgaccacgac caccagactg
     1261 tttcgcgtgt ttaccttcaa catcggtaac tttctggcgg atagtttcac ggtaagcaac
     1321 ctgcggttta cctacgttcg cttcaacgtt gaattcacgc ttcatacggt caacgatgat
     1381 gtcgaggtgc agttcgccca tacccgcgat gatggtctgg ttagattctt cgtcagtcca
     1441 tacacggaaa gacgggtctt ctttagccag acggcccaga gccagaccca ttttttcctg
     1501 gtcagctttg gttttcggtt caactgcgat ggagattacc ggctcaggga attccatacg
     1561 ttccagaatg atcggcgcat ccgggtcaca cagggtgtca ccagtggtta cgtctttcag
     1621 gccgatagca gcagcgatgt cgcccgcgcg aacttctttg atctcttcac gtttgttagc
     1681 gtgcatctga acgatacgac cgaaacgctc acgtgcagct ttcacggagt tcagtacggt
     1741 atcaccagag ttaaccacac cggagtaaac acggaagaag gtcaggttac caacaaacgg
     1801 gtcggtagcg attttgaacg ccagtgcaga gaacggctcg tcatcacttg cgtgacgttc
     1861 agccggagtg tctttaccgt cgtccaggat accgttgatc gcaggtacgt caaccgggga
     1921 tggcaggtaa tcaattaccg catccagcat cgcctgaaca cctttgttct tgaacgcaga
     1981 accacaggtt accaggatga tttcgttgtt cagaacgcgc tgacgcagag cacctttgat
     2041 ttctgcttca gtcagttctt caccacccag gtatttttcc atcagctctt cagaagcttc
     2101 agctgcggat tcgatcaggt tctggtgcca ttcgttagcc agttcaacca tgtctgccgg
     2161 gatatcttcg tattcgaagg ttacgccctg gtcagcgtca ttccagttga tagctttcat
     2221 tttcaccagg tcaacaacac cggtgaaatg ttcttcagca ccaatcgcca gctgcagcgg
     2281 aaccgggttc gcgcccagac gggttttgat ctggttaaca actttcagga agttcgcacc
     2341 cacgcggtcc attttgttaa cgaacgcaat gcgcggaact ttatatttgt ttgcctgacg
     2401 ccatacggtt tcagactgcg gctgaacacc accaactgcg cagtaaacca ttaccgcacc
     2461 atcgagaaca cgcatggaac gttctacttc gattgtgaag tcaacgtgcc ccggggtgtc
     2521 gatgatgttg atgcgatgcg gctcatactg cttagccata ccagaccaga atgcagtagt
     2581 cgcagcggaa gtgatggtaa taccacgttc ctgctcctgc tccatccagt ccatggttgc
     2641 agcgccgtca tgaacttcac cgattttatg gtttacaccg gtgtagaaca gaatacgttc
     2701 ggtagtagtg gttttaccgg cgtcgatgtg cgcactgata ccgatgttac ggtagcgtgc
     2761 gatgggtgtt gtacgagcca tttgtttcct cgtttatctt ttaggcgttc aatttaagta
     2821 gcccaaagcg ggctgcttac tggaagcgcc cgcctggtga ctaaaactcc gaagggatta
     2881 ccaacggtag tgtgcgaacg ccttgttggc ttcggccata cggtgaacgt cttcacgttt
     2941 cttaactgca gtacctttgt tttctgcagc atcagaaagt tcgttcgcca ggcgcagagc
     3001 catggattta tcaccgcgtt tacgagcagc ttcaacgatc caacgcattg ccagagcatt
     3061 acgacgaacc ggacggactt caactggtac ctgataagta gaaccaccaa cgcggcgaga
     3121 cttaacttct acagtcgggc gcacgttttc gagagctact tcgaatgctt ccagttcaga
     3181 tttaccagag cgctgagcca gggtctccag cgcgctgtat acgatagatt cagcagtaga
     3241 ttttttacca tctaccatca ggatatttac aaatttagcc agcagttctg atccgaactt
     3301 cggatccggc agaattttac gctgaccaat gacgcgacga cgtggcatgg aaatactccg
     3361 ttgttaattc aggattgtcc aaaactctac gagtttagtt tgacatttaa gttaaaacgt
     3421 ttggccttac ttaacggaga accattaagc cttaggacgc ttcacgccat acttggaacg
     3481 agcctgctta cggtctttaa cgccggagca gtcaagagca ccacgtacgg tgtggtaacg
     3541 aacacccggg aggtctttaa cacgaccgcc acggatcagg atcacggagt gctcctgcag
     3601 gttgtgacct tcaccaccga tgtaggaagt cacttcgaaa ccgttagtca gacgaacacg
     3661 gcatacttta cgcagcgcgg agttcggttt tttaggagtg gtagtatata cacgagtaca
     3721 tacgccacgt ttttgcgggc atgcttccag cgcaggcacg ttgcttttcg caactttgcg
     3781 agcacgtggt ttgcgtacca gctggttaac tgttgccatt aaatagctcc tggttttagc
     3841 ttttgcttcg taaacacgta ataaaacgtc ctcacacaat atgaggacgc cgaattttag
     3901 ggcgatgccg aaaaggtgtc aagaaatata caacgatccc gccatcacca ggccatctgg
     3961 ctggagtgct taaccgtaag tctgacgaaa tcagtatagt caatgggaat gatgtcgttc
     4021 aaaatttgac cagtcaaacc gcgggcaata aggtcttcgt tcagggcata gaccttaatg
     4081 ggggcattac gcagactttc aaggtagcgg ttaccgtcaa ctgcggcagt tacgccatct
     4141 tgcaataata gcagttcgtc tccttcactg agcagacgca gcagcgcagc aaaatccgtc
     4201 agccagggtg aacgatgtaa cgtgtgcagc ataaacgcct caaaacctca aaataacatc
     4261 gtagttggcg agttcgcggc gtaaggcatc tgcttcgagc ggcgtggctt cgacaacaaa
     4321 gggtgtctgc ggatctaacc cgcgttcgcg cagtgaagcc gcacaaaccc agcactgttc
     4381 aatgtcgtac agactcaaca atttaaaagt ggcaatgtaa tcacgcgcca gcactgcatc
     4441 gggcttttgt cctgacagca gctgaaaaac gccatcagca ataaagaaga cggccagatc
     4501 gtcagttaat gcggaagttg ccagtaaagc atctaaacct tcccgaccag cagctgtacc
     4561 atgaggtgca gtagaaaaaa caaacgcaat tcgtttcatc agaactgtac caccctgtca
     4621 caggtcagcg aggcttccgc cagcgcgcca agtccgctta aggtaaatcc cggctgaagg
     4681 tttgacgaag ccagccccag ccttcctgct tcagtttcat cgacgatacc gcggcgtaac
     4741 gctgccgcta cgcagatatt cagcgccaca ccatgttgcg cattcaactg ttgccagccg
     4801 cgcacaaggt caaattcgtc gcttgccggt gaggtcagtt ggttagcgtt atagacccct
     4861 tcccgataga aaaagacgct gcttaactta tggccttctg ctatcagcgc ctgcgcaaac
     4921 tgaaaagcac tactcgcctg ttgcgtaccg tatgctggcc cggtcaccac gatggcaaaa
     4981 cgcattactt atcttgcccc tgaaaatcac cgctcttgaa ctggcggatg tagagataga
     5041 cagtgtgttt ggagatgttc aggcggtcag caacctggtt gatcgcatct ttaatatcga
     5101 agatcccttt ctcgtagaga ttcagcacga tctgacgatt tttggcgtta ttagaaacat
     5161 tgcgatcggc attcacttct tcgatggtga actccagcgt ttgggtaacc agatcttcaa
     5221 cagaagaggc aaagttgacg cttgaaccga catccggggt ttctggcggc acaaaagtgc
     5281 tcataatctg cgagaaggga acatcaagat tcatattgat gcacagcaga ccaattacac
     5341 gctgttcgcg gttacgaatc gcgatagtca gggacttcat taatacgccg cttttggcgc
     5401 gagtaaagta gcatttagaa acgctgctat ccgctcccgt catatcgtgc agcatacgta
     5461 gcgccaggtc agtaattggc gaaccaatct tccggcctgt atgttcaccg ttagcaatgc
     5521 gaatggctga acattttaga tcctgcaaag agtgcaaaac gatttcacag tgggagccaa
     5581 taagcatcgc taacccgtcc accaccgctt cgtaggattt cagaatatca aaatcggtct
     5641 ggtcgaaagg acgttgatcc agtaaatcca actcactggt ttcgttggtt aaaagcgacc
     5701 tggacatgaa aaaaaacact ccttttcagg agcctgtcgt taacttttca gggcaggctc
     5761 attaatgatg cgggtaacta aattaataca gcggaggttc cgctttccag cactaattat
     5821 atccggcctg taataaaaaa accgccgcct ggtcaggcgg cggttcttaa tgcttatttt
     5881 ttagcagaat ctgcggcttt cgcatcagct tccggctttg catcagcctt cggcgctggt
     5941 ttcacatcca gcagctctac gtcaaacact agggtagaat tcggtgggat ccccggaaca
     6001 cccgctttgc cgtaagccag ttctggtgga ataaccagtt tgatcttacc gcctttcttg
     6061 atgttcttca gaccttctgt ccaacccggg ataacaccgt ccagacggaa agaaagcggt
     6121 tcaccacggg tgtaagagtt gtcgaactct ttaccgtcga tcagcgtacc tttgtagttc
     6181 actacaacag tatcgctgtc tttcggtgct tcgcctttac cggcttctac tacctgataa
     6241 accagaccag ttgaagaggt tttcacacct ttctctttgg caaatttctc gcggtactct
     6301 ttaccttttg cttcgttatc agccgcgtct ttttccatct tcgcctgagc agaagacttc
     6361 acgcgagctt cgaatgcttg cagagtctgt tcgatctctt ggtcggagag tttgctctta
     6421 tcagcaaatg catcctgaac accagcgatc agctgatctt tatccagttt gatgcccagt
     6481 ttttcttgtt ctttaagaga gttttccatg taacgaccca gcgaagcacc cagtgcataa
     6541 gctgatttct gatcgtcatt tttgaacgat gctttgctgt ctgcagttgt agcaggtttt
     6601 gcagcttcag cagcaaaagt gattggtgca tgcagggcaa cggccattgt ggtcgccagt
     6661 agcgttactt taaacagtga tttcatccat atctccagga tcggggcatc tcaccccagg
     6721 gttaactatt atcagaaggg tactataaag cgttgtcgaa caaatctaca tacagacacg
     6781 ccctattatc atctattttc agactctttt tgtttaaatt agtttcgatg accgcgaaat
     6841 gagtgctgtc tcgggcagca aagttaagta gaatccgcgg cgaccattcg acaaaagagg
     6901 tgaatcatgc aggatttatc attggaagca cgcctggcag agctggagag ccgactggct
     6961 tttcaggaaa tcaccattga agaactgaac gtcacggtgg ccgctcatga aatggagatg
     7021 gcgaaactgc gcgatcatct gcgtctgctg accgagaagt taaaagccag ccagccgtcg
     7081 aacatcgcgt cgcaggctga agaaacgcca ccgccacatt attgaggcgt aaaaaaagcg
     7141 gggattcccc gcttttttgt caccttttcg gtattagtgg caaccacaac cgccgttgcc
     7201 tttaccgcca cagcagcctt cgccaccgtg ttcatgaccg tgatcgtggc catgaccgcc
     7261 gcagcaaccg tcgtggtcgt gatcgtggtg atgatcgtgc gcgccgtgaa cgtggccatg
     7321 agccagttct tcttcggtcg cttcgcgaat cgccacaact tcaacgttga atttcaggtt
     7381 ctgaccggcc agcatgtggt taccatcaac cactacgtga tcgtcttcaa ccgcagtgat
     7441 ttcaaccggt accggaccct ggtcagtttc agccaggaaa cgcataccta cctgcagttc
     7501 atcaacgccc ataaatacgt ctttaggaac acgttgcact aggttttcgt cgtactgacc
     7561 gtaagcgtcg ttcgcgccaa cagcgacatc aaatttgtcg ccaacttcat gaccttccag
     7621 cgccgtttcc aggccagaga tcagggaacc gtgaccatgc aggtagtcca gcggcgcact
     7681 caccggagac tcatcaacca acacaccgtc ttctgtacgt acctgatagg ccaggctgac
     7741 caccaggtct tttgctactt tcatgatatc tcctgagcat gggaagaata gtggcgcaga
     7801 ttgtagcgga attctgcagc cgtgtactca ctagcttaaa aaaactcggc gcatatcgct
     7861 agtccggatg aaaaatcccg atcacttgct catctttgtg aacgtgatcg cgggcttctt
     7921 tgtctgcttc tcgcatctga tgtccgcact taacacattc aacaatatca atattatttt
     7981 cgcgccacat cgccattgaa tcctgcgcct gacaggccgg gcattttgcg cccgctataa
     8041 aacgttttcg gattgccatc cttaccctct actcaaattc atcccagccg tccagctggc
     8101 gtcgttcttg ttgcatttca cgctggaaaa tctcttccag ttcgcgtcgg gcttccctgg
     8161 cgcgagaaat ttgtacggta tcagcatgca tcgggattaa ctcccgcagc attcgcatat
     8221 ccaggcggcg aaaatgcagt tgcgcccgct gtgcctgatg cggatgcatg ccaagcgtga
     8281 ccagcgtctt gcgccccagc tctaacgcac tggagaatgt ttcacgggaa aactgcgtca
     8341 cccctgcctg taataactca tgcgcttcca cacgtccgcg cgctcgcgca agaatatgca
     8401 aatgcggaaa gtgctgctgg catatttcca ccagcttcat ggtgtcttcc ggctcgttac
     8461 aggtaatgac gatagactca gcggcctctg cacccgcaga acgtaaaaga tcgacctgcg
     8521 tggcgtcgcc gtaataaact ttgtagccat atttacgcat caggttaacg gcgctgatat
     8581 cccgctccag cacggtaatg cgcattttat ttgccatcag caaacgacca atcacctgac
     8641 caaaacgccc gaagcccacg acaatgacct ggggtttatc atcgttgacc cacggttttt
     8701 cgtcttcttc ttccggtccg ttaaactggc gggatagcca tttatccacc agcttcatca
     8761 gcaacggcgc catctggtcg ccctggaata aacgttgtga agaagcggta gaaaagagga
     8821 caaaggcaaa ctcaccgccc tgactcaaca cgccagcaaa ctgcatccgc tctgagctac
     8881 gcacgccata caatcgcgcc agcagataca gcacgagaat tttcaccgcc accagcacaa
     8941 ccacgcttat cactacccac aacagatggg tataaagcac cccgaggttg agcgacatgc
     9001 cgacagagat aaagaacaaa ccgagcagca agcctttgaa gggatcgata gccgtttcca
     9061 gttcatggcg atattcactt tccgccagca gcacgcccgc aataaatgta ccgagcgcca
     9121 tcgacagccc cagcgcatcc ataaacaatg cggaacccaa caccagcagc agcgtcgcgg
     9181 cggtgaacac ttcccgcacg ccagaagctg caataaagcg gaataccgga cgcagtaaat
     9241 agcgcccacc aatcagcatg ccgacaaact ccagcacctt catgccgatc ttcatccagt
     9301 cgaaatgttc gtctgccgac cccgccaaca acggcactaa cgccagtgct gggatcactg
     9361 ccagatcctg aaacagcaga accgaaaacc cgagctggcc ggattcgctg cgattcatcc
     9421 ctttctcacg catcaattgc aacgccattg cagttgaaga catcgcaaag ccaatgccac
     9481 cgaccaccgc cgcctgccag gcgaaatccg tcagcatcaa taatcccgcc agcaacgccg
     9541 cgcttaacag cacctgtgcc gcgcctacgc caaaaatcga acgccgcagt tgccaaagtt
     9601 tggagggatt caactcaagg ccgatgataa acatcaggaa taccacgccg agttccgaaa
     9661 agtggaggat ctcatcgacg tcgctaataa accccagccc ccacgggcca attgcaatcc
     9721 ccgccagcaa atatcccaac acagcgccaa tccccagccg cgaagagaaa cagcactcct
     9781 gcgagtaaaa aatcggaacc ttccatcagc ggcctcctgg agacagcgga tttgccaacc
     9841 agtcaccgta ggctctggcg tggctcgcca gctcctttgc gctttgccgt ctcgcccagt
     9901 aaataatgat gggacttaac caatgcatcc ggcacatgcc cgccgccagt tcaaaggggc
     9961 gcagcacatc gctcatcggg tagcgattca gcgcgtcata acggtaagca ctttccggct
    10021 cgccggtggt aatcacgtta cgccagtact ttcccgccag ttggtttcct cccggcccgc
    10081 tggcaaaacc acgacttaat acccggtcca gccactcttt cagtagcgcc gggcagctat
    10141 aggtataaag aggatgctga aagacaatca cctcgtgctc gcgcagtaat gcctgctcac
    10201 gggggatatc aataaaaaaa tcgggatagt gcgcgtaaag gtcgtgcacg gtaacattgc
    10261 tgagctgcgt ggccggttta agcagtaccc ggtttgccac cgagtcctga gattccggat
    10321 gggcatacag cagcaaaact ttcgctggct gagacatcat ccccctcccg gtatggtttt
    10381 tgtgtatagt cgctgttttg ggctaccatt gcgcccggtg cggcagctcg cccatacatt
    10441 acattatcat aatgataagt taacatagtc tgaacatacg gcgccttatg attgttttct
    10501 cctcgttaca aattcgtcgc ggcgtgcgcg tcctgctgga taatgccacc gccaccatca
    10561 accccgggca gaaagtcggc ctggtgggta aaaacggctg tggtaaatct accctgctgg
    10621 cattgctgaa aaatgaaatc agcgccgacg gcggcagcta cacctttccg ggaagctggc
    10681 aactggcgtg ggtgaatcag gaaacgccag cgttaccgca agcggcgctg gaatatgtca
    10741 ttgacggcga ccgtgaatat cgtcaactgg aagcgcagct gcacgacgcc aacgaacgta
    10801 acgacgggca cgccattgcg accattcata gcaagctgga tgctattgac gcatggagta
    10861 ttcgctcccg tgccgccagc ctgctgcacg gcctcggttt cagcaatgaa caactggagc
    10921 gcccggtaag tgatttctcc ggtggctggc gtatgcgtct taaccttgcc caggcgctga
    10981 tttgccgttc agacttgctg ctgctcgacg aaccgactaa ccacctcgat ctcgatgccg
    11041 ttatctggct ggaaaaatgg ttgaagagct atcagggcac gctgatcctg atctctcacg
    11101 accgcgactt cctcgatccg atcgttgata aaattattca tatcgaacaa caaagcatgt
    11161 tcgagtacac cggcaactac agttcgtttg aagtacagcg cgccacccgt ctggcgcagc
    11221 aacaagcgat gtacgaaagc cagcaggaac gcgtggcaca tctgcaaagt tatatcgatc
    11281 gtttccgtgc caaagccacc aaagcgaagc aggcccagag ccgcattaag atgctggaac
    11341 gtatggagct gattgccccc gcacacgtcg acaacccgtt ccgctttagc ttccgcgcgc
    11401 cggaaagcct gccaaatccg ttactgaaga tggaaaaggt cagcgcaggc tatggcgatc
    11461 gcattattct cgactcgatt aaattgaatc tggtccccgg ctcgcgcatt ggtctgttag
    11521 gccgcaacgg cgcaggtaaa tcgacattaa tcaaactgtt agccggtgaa cttgcgccag
    11581 tcagcggtga aattggtctg gcgaaaggga tcaagctcgg ctacttcgcc cagcatcaac
    11641 ttgaatacct gcgcgccgac gaatcaccta ttcaacatct ggcacgttta gcgccgcagg
    11701 agctggaaca aaaactgcgt gactacctcg gcggctttgg tttcctgggc gataaagtaa
    11761 ccgaagaaac gcgccgcttc tccggtgggg aaaaagcccg cctggtgctg gcattaattg
    11821 tctggcagcg tccgaatctg ctgctgctcg acgaaccgac taaccacctt gacctcgaca
    11881 tgcgtcaggc actcaccgaa gcattaatcg agtttgaagg cgcgctggtt gtcgtttcgc
    11941 acgaccgtca tttgctgcgt tccaccactg acgatctcta cctggttcac gatcgtaaag
    12001 tcgaaccgtt cgacggcgat ctggaagatt atcaacagtg gttgagcgac gtacaaaagc
    12061 aggaaaacca gaccgacgaa gcgccaaaag agaacgcgaa cagcgcccag gcacgtaaag
    12121 atcagaagcg tcgggaagcg gagctgcgtg cgcaaaccca gccgctgcgt aaagagattg
    12181 cccgtctgga aaaagagatg gagaagctga acgcgcaact ggcgcaggcg gaagagaaac
    12241 tcggcgacag cgaactgtat gaccagagcc gtaaagcgga gttgaccgcc tgcctgcaac
    12301 agcaagccag cgccaaatcc ggcctggaag agtgcgaaat ggcgtggctg gaagcccagg
    12361 agcagcttga gcagatgctg ctggaaggcc aaagcaactg atggcgcaga taacgacgac
    12421 cgatgccaat gaatttagca gcagtgctga attcacccct atgcgcggct ttagcaattg
    12481 tcatctacaa actatgctgc cgcgtttgtt tcgtcgtcag gtgaaattca ccccgtactg
    12541 gcagcggctg gagttgcccg acggcgattt tgtcgatctc gcgtggagtg aagaccccgc
    12601 acaggcgaaa cataaaccgc gtttagtggt gtttcacggg ctggaaggca gtctcaatag
    12661 cccttacgcc cacggtctgg ttgaggcggc gcaaaagcgc ggctggctgg gcgtggtgat
    12721 gcattttcgc ggatgcagcg gtgaaccaaa ccgtatgcac cgcatttacc attcgggcga
    12781 aaccgaagac gccagttggt ttttacgctg gctgcaacgc gaatttggtc atgcgccaac
    12841 ggctgccgtc ggctattcgc tcggcggtaa tatgctggcc tgtttgctgg caaaggaagg
    12901 caatgatctc ccggttgatg cggcggtgat tgtctctgcg ccgtttatgc tggaagcctg
    12961 tagctatcat atggaaaagg gcttttcccg cgtttatcag cgttacttgc tgaacctgtt
    13021 aaaagccaat gccgcgcgca agctggcagc ctaccccgga acgctgccga ttaatctcgc
    13081 gcagttaaaa tcggtacgtc gcatccgtga atttgacgat ctaatcaccg ccagaattca
    13141 cggctacgcc gacgctatcg actattatcg tcagtgtagc gccatgccaa tgctgaaccg
    13201 gatcgccaaa ccgacgctga ttattcacgc caaagacgat ccgtttatgg atcatcaggt
    13261 gatcccaaaa ccggaaagtc tcccctcgca ggtggagtat caactgactg aacatggcgg
    13321 tcatgttggc tttattggcg gtacattact tcatccgcaa atgtggctgg agtcacgcat
    13381 tcctgactgg ttaacaacgt atctggaggc gaaatcatgt tgattccgtg gcaagacctc
    13441 tcccccgaaa cgctggaaaa tttaattgaa agctttgtgt tacgtgaagg caccgattat
    13501 ggtgaacatg agcgtacact tgaacagaaa gtcgccgacg tcaaacgcca gctacagtgc
    13561 ggagaagcgg tgctggtatg gtcggagctg cacgaaacgg tcaatattat gccgcgcagt
    13621 cagtttcgcg aataacctgc aaccgtcggc ggacgagata atagtgatag ctaagtcatg
    13681 gagttactat gtctgccaaa catccggtca ttgcggtaac aggatccagc ggcgcgggga
    13741 ccaccaccac cagcctcgcg tttcgtaaaa tattcgcgca gttaaatctg catgcagctg
    13801 aggtggaagg cgacagtttt caccgttaca cccgcccgga aatggacatg gcgatccgca
    13861 aagcgcgcga cgccgggcgg catatcagct acttcggccc cgaagcgaac gacttcggcc
    13921 tgctggaaca aaccttcatt gaatacggtc agagcggcaa agggaaatct cgcaaatatc
    13981 tgcataccta cgacgaagcc gtaccctgga atcaggtacc gggaacattc accccctggc
    14041 aacctttacc agaacccact gatgtactgt tttatgaagg tttacacggc ggcgtagtca
    14101 cgccacagca taacgttgcg cagcatgtgg acttactggt cggcgtagtg cctatcgtta
    14161 accttgagtg gattcaaaaa ctgatccgcg acaccagcga gcgcgggcac tcacgagaag
    14221 cagtgatgga ctcggtagtg cgttcaatgg aagactatat caactacatc acaccgcagt
    14281 tttcccgcac ccatcttaac ttccagcgcg ttcccaccgt cgacacttca aacccgttcg
    14341 cggcaaaagg tatcccgtcg ctggatgaaa gctttgtggt gatccatttt cgtaatctgg
    14401 aagggatcga tttcccttgg ctgctggcga tgttgcaagg ctcattcatt tcccacatca
    14461 atacgttagt ggtacctggc ggcaaaatgg ggctggcaat ggaattaatt atgctgccgc
    14521 tggtgcaacg attgatggaa ggaaagaaaa tcgagtaact ttgctattat gccggataaa
    14581 atactatccg gcttcacaac gggatagtta agtcacgcgg caaccacttc atacgagtga
    14641 gtaatattca ccgctttttc cagcatcaac gccactgaac aatatttctc ggcagagaga
    14701 tcaaccgcac gcgcaaccgc tgcgtctttc aggtcgcgac cggtgacgat aaaatgcaga
    14761 ttaatgtgcg taaacaggcg tggtgcctct tcgcggcgtt cagaggtcaa ttttacttca
    14821 caatcgacca catcctgacg ccctttttgc aggatcgaaa ccacatcgat ggcactgcaa
    14881 ccacccgccg ccatcaacac catttccatc ggacttggtg ctttatcgcc tgagttgccg
    14941 tccattaaaa tctggtgacc agaggcggat tcgcccagaa aagttaaccc ttcgacccac
    15001 ttcactcgcg cttgcatttt tgctactcca ctgcgtcaat tttcctgaca gagtacgcgt
    15061 actaaccaaa tcgcgcaacg gaaggcgacc tgggtcatgc tgaagcgaga caccaggaga
    15121 cacaaagcga aagctatgct aaaacagtca ggatgctaca gtaatacatt gatgtactgc
    15181 atgtatgcaa aggacgtcac attaccgtgc agtacagttg atagcccctt cccaggtagc
    15241 gggaagcata tttcggcaat ccagagacag cggcgttatc tggctctgga gaaagcttat
    15301 aacagaggat aaccgcgcat ggtgcttggc aaaccgcaaa cagacccgac tctcgaatgg
    15361 ttcttgtctc attgccacat tcataagtac ccatccaaga gcacgcttat tcaccagggt
    15421 gaaaaagcgg aaacgctgta ctacatcgtt aaaggctctg tggcagtgct gatcaaagac
    15481 gaagagggta aagaaatgat cctctcctat ctgaatcagg gtgattttat tggcgaactg
    15541 ggcctgtttg aagagggcca ggaacgtagc gcatgggtac gtgcgaaaac cgcctgtgaa
    15601 gtggctgaaa tttcgtacaa aaaatttcgc caattgattc aggtaaaccc ggacattctg
    15661 atgcgtctgt ctgcacagat ggcgcgtcgt ctgcaagtca cttcagagaa agtgggcaac
    15721 ctggcgttcc tcgacgtgac gggccgcatt gcacagactc tgctgaacct ggcaaaacaa
    15781 ccagatgcta tgactcaccc ggacggtatg caaatcaaaa ttacccgtca ggaaatcggt
    15841 cagattgtcg gctgttctcg tgaaaccgtg ggacgcattc tgaagatgct ggaagatcag
    15901 aacctgatct ccgcacacgg taaaaccatc gtcgtttacg gcactcgtta atcccgtcgg
    15961 agtggcgcgt tacctggtag cgcgccattt tgtttccccc gatgtggcgc agactgattt
    16021 atcaccccga tatcaactat gcacttcgac aaacgctggt gctatgtttg cccgtggccg
    16081 ttgggttaat gcttggcgaa ttacgattcg gtctgctctt ctccctcgtt cctgcctgtt
    16141 gcaatattgc gggccttgat acgcctcata aacgtttttt caaacactta atcattggtg
    16201 cgtcgctgtt tgccacctgt agcttactga cacagctgct actggtaaaa gatgttcccc
    16261 tgcccttttt gctgaccgga ttaacgctgg tacttggcgt cactgctgag ctggggccat
    16321 tacacgcaaa attgcttcct gcatcgctgc tcgccgccat ttttaccctc agtctggcgg
    16381 gatacatgcc ggtctgggaa ccattgctca tctatgcgtt gggcactctc tggtacggat
    16441 tgtttaactg gttttggttc tggatctggc gcgaacaacc gctgcgcgag tcactaagtc
    16501 tgctgtaccg tgaactggca gattattgtg aagccaaata cagcctgctt acccagcaca
    16561 ccgatcctga aaaagcgctg ccaccgctgc tggtgcgcca gcaaaaagcg gtcgatttaa
    16621 ttactcagtg ctatcagcaa atgcatatgc tttccgcgca aaacaatacc gattacaagc
    16681 ggatgctgcg tattttccag gaggcgctgg atttgcagga acatatttcg gtcagtttgc
    16741 atcagccgga agaggtgcaa aaactggtcg agcgtagcca tgcggaagaa gttatccgct
    16801 ggaatgcgca aaccgtcgcc gctcgcctgc gcgtgctggc tgatgacatt ctttaccatc
    16861 gcctgccaac gcgttttacg atggaaaagc aaattggcgc actggaaaaa atcgcccgcc
    16921 agcatccgga taatccggtt gggcaattct gctactggca tttcagccgc atcgcccgcg
    16981 tgctgcgcac ccaaaaaccg ctctatgccc gtgacttact ggccgataaa cagcggcgaa
    17041 tgccgttgct tccggcactg aaaagttatc tgtcactaaa gtctccggcg ctacgcaatg
    17101 ccggacgact cagtgtgatg ttaagcgttg ccagcctgat gggcaccgtg ctgcatctgc
    17161 cgaagtcgta ctggatcctg atgacggtat tgctggtgac acaaaatggc tatggcgcaa
    17221 cccgtctgag gattgtgaat cgctccgtgg gaaccgtggt cgggttaatc attgcgggcg
    17281 tagcgctgca ctttaaaatt cccgaaagtt acaccctgac gttgatgctg attaccaccc
    17341 tcgccagcta cctgatattg cgcaaaaact acggctgggc aacggtcggt tttactatta
    17401 ccgcagtgta taccctgcaa ctgttgtggc tgaacggtga gcagtacatc cttccacgtc
    17461 ttatcgatac cattattggt tgtttgattg ctttcggtgg taccgtctgg ctgtggccgc
    17521 agtggcagag cgggttattg cgtaaaaacg cccatgatgc tttagaagcc tatcaggaag
    17581 cgattcgctt gattctgagc gaggacccgc aaccaacgcc actggcctgg cagagaatgc
    17641 gggtaaatca ggcgcataac actctgtata actcattgaa tcaggcgatg caggaaccgg
    17701 cattcaacag ccattatctg gcagatatga aactgtgggt aacgcacagc cagtttattg
    17761 ttgagcatat taatgccatg accacgctgg cgcgggaaca ccgggcattg ccacctgaac
    17821 tggcacaaga gtatttacag tcttgtgaaa tcgccattca gcgttgtcag cagcgactgg
    17881 agtatgacga accgggtagt tctggcgatg ccaatatcat ggatgcgccg gagatgcagc
    17941 cgcacgaagg cgcggcaggt acgctggagc agcatttaca gcgggttatt ggccatctga
    18001 acaccatgca caccatttcg tcgatggcat ggcgtcagcg accgcatcac gggatttggc
    18061 tgagtcgcaa gttgcgggat tcgaaggcgt aatacaggct aaaccatcgg ataaggcatt
    18121 cacgccgcat ccgacatatt ttgcctgata cgtcttattt acgccccaac caccttcgcc
    18181 accgcgtggg cgaaacgttg catcccttca tcgatatccg catcttccac caccagcgac
    18241 ggtgctaaac gcatcacatc cggtccggca ttcagcacca ttacgccaac ctctgcaccc
    18301 gcatacagga aatcacgcgc ccgacctttg tagtgtggtt tcagctccgc gccaattaac
    18361 agccccatac cgcgaatatc gctaaacaca tcgtactgct gatcgatctt ctgcaaatgg
    18421 tcaacaaaac gctggcgttt cgcctgaatg ccttccagca cttcaggggt attgatgata
    18481 tcaaacgccg cgcccgctac tgcacaggcc agaggattac cgccgtaggt ggaaccgtga
    18541 gaaccaggat gaaacgcaga agcaatttcc gccgtggtca gcatggcgct aatcgggaag
    18601 ccgccgccta acgctttcgc agaagtcaga atatccggcg tcacaccgta gtacctgtaa
    18661 gcaaacaaat cgccagtccg ccccatcccg cactgcactt catcaaacac caataatgcc
    18721 tgatgttgat cgcacagctc gcgcaagccc tgcaaaaact ctggcgtcgc tgccgtcaca
    18781 ccgccctcgc cctggatcgg ctcaaccacc accgcacagg tgtgatcatc catcaccgct
    18841 ttcactgcat ggagatcgtt aaagggaacg tggatgatgt ctgccggttt cggcccaaag
    18901 ccgtcggaat attttggctg cccacccacc gaaacggtaa acagcgagcg accatgaaaa
    18961 gcgttatgga aggcaataat tttggttttg aacgggctat gacgcacaca ggcgtaatgg
    19021 cgtgccagtt taaaggcggt ttcgttagct tccgtgccgg agttcataaa caccacgcgt
    19081 tcggcaaacg tcgcctcaat cagtttgcgc ccaagacgca gcgccggttc attggtgaaa
    19141 acgttactga tatgccacag agtttcgccc tgggttttta acgcgttcac cagcgcagga
    19201 tggcaatggc ccaacgccgt aactgcaatg ccacccgcga aatcgacata ctccttgcct
    19261 tgctgatccc agattcggct gccctgacct tttaccggaa taaactctgc cggagcataa
    19321 atcggcagga tcacttcatc gaaagtcgcg cgtgtaattg ctgtttgttc aattgccatc
    19381 tcatgatcac cctgttacgc ataaataaat gtgaaattat aatcacaaaa tatgcataaa
    19441 aaatcactga atggcaatcg gaaatcagag atgcaggaaa ttagccagca gttgatgtcc
    19501 ttgttcgcta agaatacttt ctggatggaa ctgcacacct tccagatccc actggcgatg
    19561 gcgaatcccc ataatctcgc gggtttcgct ccaggccgtc acgtcaaagc acgctggtaa
    19621 tgagtcaggt tccaccacca gcgaatggta gcgtgtcacg gtaagtggat ttgccagccc
    19681 ccgaaatacg ccctcaccgc tatgtgtaat cggcgaggtt ttgccgtgca tgacctttgc
    19741 ggcgcgcaca actttgccgc caaatgcctg cgccattgcc tgatgaccga ggcagacgcc
    19801 aagaatcggc aagcgcccgg catagtggcg aataacgtca agggagatcc cggcttcatc
    19861 tggcgtacag gggccaggtg agatgacaat tttttgtggt ttaagggcgt cgatatccgc
    19921 cagcgtcaac gcatcgttgc gcttaaccag cacatccgcc cccagttcac aaaagtactg
    19981 gtagaggttc caggtaaaag aatcgtagtt atctataagc aggatcatgg cggctccggt
    20041 acgaaagaac cgcgctattt tactcagatt ccccggcttc gcttaccact ttgctaaata
    20101 tcatctgcag tgcggtgaga tcccccattg ccccactctg atttgcctga ttccaggcct
    20161 ctttttcgat accctgccag ctcagttgat atccggcatg aatcgccagt tgctcgaaga
    20221 agatccgctg tgccagacca cttcccaccc ggaagggatg cagcacgttg atttcacaat
    20281 agtaatgcgc cagccgctcg acgaacttcg ctttctccag gccaaccaga taaccttctt
    20341 cctccagatc ctgcatcagg gcattgccct ctttttcgat ataagcaaag tggcagaacg
    20401 gcgtatcgcc ctgataaata tcaacttcac gcagttgccc tgcccagtcg aaaatatcct
    20461 ggtacagctg gcgatggata gtgcgcaaat gcggtaaacc gcgcaccagc ggaccaagct
    20521 caatggtcgc agcacgcagc gccgtcattt cgtaagcggc ctgttccagc cgctgctgct
    20581 ggcggatgtt cagccggtta cgcatgatat caaggccagg ataaagatac agatcgcgcc
    20641 cttcgccgaa tttatcgctc atagtgcctc ctcagctctt caaggcgcgc taaagcctct
    20701 gcggcagtaa gagtgactaa aggcatctcg acgccttcaa gacggcgact ggcctggaaa
    20761 ttacgattac gctgaagctc ccagagacgg gacttttgct tatcggtgag tttcttcact
    20821 tgatgcctcc ctgaacgtgt ccatttgcca caagtataag cggcaaatcc gggttacgcc
    20881 gggagagcag aagcgcgggc aagattgccc gcgcgagaaa tcattacggc aggactttag
    20941 cggaaaggat aactaccggt tttgacggca cattctggta cggaccaaca tcatgagtcg
    21001 gcacctggga aatcttatcg gcaacgtcca tgcctttcac cactttacca aataccgcgt
    21061 aaccgaaatc acgctgacca tggtcaagga aggcgttatc ggcaacgttg ataaagaact
    21121 ggctggtggc gctgtctttg tcagcggtac gcgccatcgc gatggtgcca cgcgtgttgc
    21181 gcaggccgtt atcggcttca tttttgattg gcgggtttgg ttttttctgc tgcatctgct
    21241 cggtgaaacc gccgccctga atcataaagc caggaatgac gcggtgaaag gtagtgttgt
    21301 tataaaaacc gctgttcaca taatcgacaa agttttgcac agacactggc gctttttgtt
    21361 tatccagctc cagttcgatg ttaccagctg aggttgtcaa caatacgtgc gggtcccctt
    21421 tcgctgccat tgctgcggga gaaagagcag aaagagcaga aagagcgaaa acagcagcca
    21481 tcgccgccag ggtcgatttg aacatgagat ttccttaaca aacagagcag aaaaaaagca
    21541 agtgaagaga ttctaaatag ccttcaagaa caaggccatc cctttaccta attttacgta
    21601 tctgaaatat atgtaacctg acgatcactt tttattccgt gatcaaaatc acctcttaaa
    21661 atgcaattta gcaaccgatt gcaataaaac atttaaacag atcataaaat cacctaaaat
    21721 cgcccgctcg cggcatatcc cgctatgcca ttttattttt gaacgcaggc caatcatgac
    21781 taacagcaat cgcatcaagc tcacatggat tagctttctc tcctacgcac tgactggtgc
    21841 gttggttatt gtcaccggga tggtgatggg aaatatcgcc gattatttca atctgcctgt
    21901 ttccagtatg agtaacacct tcaccttcct caacgccggc attttaatct ctatcttcct
    21961 caacgcctgg ctgatggaaa tcgtcccgtt gaaaacgcag ttacgttttg gctttctcct
    22021 gatggtgctg gcggttgccg gtttgatgtt cagccacagc ctggcgctgt tctcgacggc
    22081 gatgttcatt ctcggggtgg tcagcggcat tactatgtcg attggtacat tcctgataac
    22141 acaaatgtat gaagggcgtc agcgcggttc ccgcctgtta tttactgact ccttcttcag
    22201 tatggctggg atgattttcc caatgatcgc cgcgtttcta ctggcgcgca gcattgagtg
    22261 gtactgggtt tatgcctgca tcgggctggt gtatgtcgct atttttattc tgaccttcgg
    22321 ctgtgagttc ccggcactgg gcaaacgtgc accaaaaacg gatgctccgg tagagaaaga
    22381 aaagtggggg atcggcgtac tgtttctctc tgttgcggcg ctgtgctaca tcctcggtca
    22441 gttaggtttt atctcctggg tgcctgagta tgccaaaggc ctgggcatga gcctgaacga
    22501 cgcgggcacg ctggtgagta acttctggat gtcatacatg gtcggcatgt gggcgttcag
    22561 ctttattctt cgcttctttg atttgcaacg cattctgacc gtactggctg gtctggctgc
    22621 gattctgatg tacgtcttta acaccggaac accagtacat atggcgtggt caattctcgc
    22681 tctgggcttc ttctccagcg cgatctatac caccatcatc accctgggtt cacagcagac
    22741 caaagtaccg tcgccaaaac tggttaactt tgtcctgacc tgcggaacca tcggtactat
    22801 gttgaccttt gtggttaccg gcacgattgt tgaacatagc ggtccgcagg cggcactgct
    22861 tacagcaaac ggtctgtacg ctgtcgtctt tgtgatgtgc ttcctgttag gtttcgtcag
    22921 ccgtcaccgt cagcataaca ccctgacctc tcattaattg ctaatgccgg acggcactat
    22981 cgtcgtccgg ccttttcctc tcttcccccg ctacgtgcat ctatttctat aaacccgttc
    23041 attttgtcca ttttttgcac aaacatgaaa tgtcagacaa ttccgtgact taagaaaatt
    23101 tatacaaatc agcaatatac cccttaagga gtatataaag gtgaatttga tttacatcaa
    23161 taagcggggt tgctgaatcg ttaaggtagg cggtaataga aaagaaatcg aggcaaaaat
    23221 gagcaaagtc agactcgcaa ttatcggtaa cggtatggtc ggccatcgct ttatcgaaga
    23281 tcttcttgat aaatctgatg cggccaactt tgatattacc gttttctgtg aagaaccgcg
    23341 catcgcttat gaccgcgtac acctctcatc ttacttctct caccacaccg ccgaagagct
    23401 gtcgctggtg cgcgaaggct tctacgagaa acacggcatc aaagttctgg tcggcgaacg
    23461 cgctatcacc atcaaccgtc aggagaaggt gattcactcc agcgccggac gtaccgtttt
    23521 ttatgacaag ctgatcatgg caaccggttc ctacccgtgg atcccaccaa tcaaaggttc
    23581 tgatactcag gactgctttg tctatcgcac cattgaagac ctcaacgcca ttgaatcctg
    23641 cgcccgtcgc agcaaacgcg gtgccgttgt tggtggcggc ctgttaggtc tggaagccgc
    23701 tggcgcgctg aaaaacttag gtattgaaac ccacgttatc gaatttgccc ctatgctgat
    23761 ggcggaacag cttgatcaga tgggtggcga gcagctgcgt cgcaaaatcg aaagtatggg
    23821 cgtgcgcgtt cacaccagca aaaacaccct tgagattgtg caggaaggtg tcgaagcgcg
    23881 taaaaccatg cgttttgccg acggcagcga actggaagtc gactttatcg tcttctctac
    23941 cggtatccgt ccgcgcgata agctggcaac ccagtgtggt ctggacgttg ctccgcgtgg
    24001 ggggattgtc attaatgatt cctgccagac ttccgatccg gatatctacg ccatcggtga
    24061 atgcgcaagc tggaacaacc gtgtatttgg tctggtagca ccaggctaca aaatggcgca
    24121 ggtcgccgtt gaccatattc tgggtagcga aaacgccttt gaaggtgctg accttagcgc
    24181 caagctgaaa ctgctgggcg tagacgtagg tggtattggt gatgcgcacg gtcgcacgcc
    24241 tggcgcacgt agctatgttt acctcgacga aagtaaagag atctacaaac gcctgattgt
    24301 cagcgaagac aacaaaaccc tgctcggtgc ggtactggtg ggcgatacca gcgactacgg
    24361 caacctgctg caactggtgc tgaacgctat cgagctgccg gaaaacccgg attctctgat
    24421 tctgccggcg cactcgggta gcggtaagcc gtctatcggt gttgataaac tgccggacag
    24481 cgcgcaaatc tgctcctgct tcgacgtcac taaaggtgat ctgattgctg ccatcaacaa
    24541 aggctgccac acagttgcgg cgctgaaagc tgaaaccaaa gcgggtactg gctgcggtgg
    24601 ctgtatcccg ctggtcactc aggtactgaa cgcggaactg gcgaaacagg gcatcgaagt
    24661 taacaacaac ctgtgcgaac actttgctta ttcgcgtcag gaactgttcc atttgatccg
    24721 cgttgaaggc attaaaacct tcgaagaact gctggcgaaa cacggcaaag gctacggttg
    24781 tgaagtttgt aaaccaaccg tcggttcgct gctggcctcc tgctggaacg aatacattct
    24841 gaagccggaa catactccgc tgcaggattc taacgacaac ttcctcgcta acatccagaa
    24901 agacggcacc tactcggtga tcccgcgttc tccgggcggt gaaatcaccc cggaagggct
    24961 gatggcagtg ggtcgtatcg cgcgtgaatt taatctctac accaaaatca ccggctccca
    25021 gcgcctggcg atgtttggcg cacagaaaga cgatctgccg gaaatctggc gtcagctgat
    25081 tgaagctggc ttcgaaaccg gtcatgccta tgcgaaagca ctgcgtatgg cgaaaacctg
    25141 cgtgggtagc acctggtgcc gctacggtgt tggcgatagc gtcggcctcg gcgtggaact
    25201 ggaaaaccgc tacaaaggca tccgtacgcc gcacaaaatg aagttcggtg tctccggctg
    25261 tacccgtgaa tgttcagaag ctcagggtaa agacgtgggt attatcgcca ctgaaaaagg
    25321 ctggaacctg tatgtttgcg gtaacggcgg catgaaaccg cgtcatgcgg atctgctggc
    25381 agcggatatc gatcgcgaaa cgctgatcaa atatctcgac cgcttcatga tgttctacat
    25441 ccgtaccgcc gacaaactga cgcgtaccgc gccgtggtta gaaaacctcg aaggcggcat
    25501 cgattacctg aaagcggtga tcattgacga caaactgggg ctgaacgctc atctggaaga
    25561 agagatggcg cgcctgcgtg aagcggtagt gtgtgagtgg actgaaacgg tcaatacacc
    25621 gtccgcgcag actcgcttca aacacttcat caacagcgac aagcgtgacc cgaacgtgca
    25681 gatggtgcca gagcgcgaac agcaccgtcc ggcaacgccg tatgaacgta tcccggtaac
    25741 tctggtggag gacaacgcat gagccagtgg aaagacatct gcaaaatcga tgacatcctg
    25801 cctgaaaccg gcgtctgcgc actgttaggt gacaagcagg tagcgatttt ccgcccgtat
    25861 cacagcgacc aggtgtttgc gatcagcaac attgatccgt tcttcgagtc cagcgtgcta
    25921 tcacgcgggc tgattgcgga acaccagggc gagctgtggg tcgccagccc gctgaaaaaa
    25981 cagcgttttc gcttaagtga tggcttgtgc atggaagacg aacagttttc cgtcaaacat
    26041 tacgaagcac gagtgaaaga cggcgtggtg cagctgcgcg gttaatgttt taacgggagg
    26101 cgcaatgcct cccctttttg catggtcctg taatgacctt cggtatattg caggacattt
    26161 tttaaacttt ttgttttatt ttttgttttt attttttaaa ggataatcaa atgtttacag
    26221 acactattaa taagtgtgcg gctaatgctg cgcgcattgc acgcctgtcg gcaaataacc
    26281 cgctcggctt ttgggtcagc tccgccatgg cgggcgcata tgtgggtctt gggatcatcc
    26341 tgattttcac gctcggtaat ttgctcgacc cgtccgtacg tccgctggtg atgggcgcga
    26401 cctttggtat cgccttaacg ctggtgatta tcgccggttc tgaactgttc accggacaca
    26461 ccatgttcct cacctttggg gtaaaagcgg gcagcatcag ccacgggcaa atgtgggcaa
    26521 tcctgccgca aacctggctg ggtaacctgg tcggttccgt cttcgttgcc atgctctata
    26581 gctggggcgg cggtagcctg ctgccggtag ataccagcat cgttcactcc gtcgcgctgg
    26641 ctaaaaccac tgcaccggca atggtactgt tcttcaaagg tgcattgtgt aactggctgg
    26701 tttgcctggc aatctggatg gcgctgcgca ctgaaggggc ggcgaaattt atcgctatct
    26761 ggtggtgtct gctggcattt atcgcgtccg gctacgagca ctctatcgct aacatgacgc
    26821 tgttcgcgct ctcctggttc ggcaaccaca gcgaagccta cacgctggcg ggtattggtc
    26881 ataacctgct gtgggtgacg ctgggtaata ctttatcagg tgccgtattc atgggattgg
    26941 gttattggta tgctacgccg aaagcgaatc gtccggttgc ggacaaattt aatcaaactg
    27001 aaacagctgc cggttaatta ctaaggggtt tttacgtgga tcatttgcct atattttgcc
    27061 aattacgcga tcgcgactgt ctgattgtcg gcggtggtga tgtcgcggaa cgcaaagcaa
    27121 ggttgctgtt agacgcaggc gctcgcttaa cggtgaatgc attagcgttt attccacagt
    27181 tcaccgcatg ggcagatgca ggcatgttaa ccctcgtcga agggccattt gatgaaagcc
    27241 ttctcgacac ctgctggctg gcgattgcag cgacggatga tgacacgctt aaccagcgcg
    27301 tcagcgaagc cgctgaagct cgtcgcatct tctgtaacgt agttgatgcg ccgaaagccg
    27361 ccagctttat tatgccgtcg attattgacc gctcaccgct aatggtagcg gtctcctctg
    27421 gcggcacctc tccggttctg gcgcgcctgt tgcgcgaaaa actcgaatca ctgctgccgt
    27481 tgcatctggg ccaggtggcg aaatatgccg ggcaattacg cgggcgagtg aaacaacagt
    27541 tcgccacgat gagtgagcgt cgccgtttct gggagaaatt gttcgttaac gaccgcctgg
    27601 cgcagtcgct ggcaaacaac gatcagaaag ccattactga aacgaccgaa cagttaatca
    27661 acgaaccgct cgaccatcgc ggtgaagtgg tgctggttgg tgcaggtccg ggcgatgccg
    27721 ggctgctgac gctgaaagga ctgcaacaaa ttcagcaggc agatgtggtg gtctacgacc
    27781 gtctggtttc tgacgatatt atgaatctgg tacgccgcga tgctgatcgc gttttcgtcg
    27841 gcaaacgcgc gggataccac tgtgtaccgc aggaagagat taaccagatc ctgctgcggg
    27901 aagcgcaaaa aggcaaacgc gtggtgcggc tgaaaggtgg cgatccgttt atttttggcc
    27961 gtggtggcga agagctggaa acactgtgca acgcgggtat tccgttctcg gtggttccgg
    28021 gtattaccgc agcttctggt tgctctgcct attcgggtat tccactcacg catcgcgatt
    28081 atgcccagag cgtacgctta attaccggac acttaaaaac cggtggcgag ctggactggg
    28141 aaaacctggc ggcagaaaaa cagacgctgg tgttctatat ggggttgaat caggccgcga
    28201 ctattcagca aaagctgatt gaacacggta tgcctggcga aatgccggtg gcaattgtcg
    28261 aaaacggaac ggcagtcacg cagcgcgtga ttgacggtac gctcacgcaa ctcggtgaac
    28321 ttgctcagca aatgaacagt ccatcgctaa ttattattgg tcgggttgtt ggcctgcgcg
    28381 ataaactgaa ctggttctcc aatcattaat ttaacccggg ccagagaatt ctggccttct
    28441 taatgactct ttttatatat tcagcaaata aaacataacc ccttaataaa tattatctgc
    28501 taatgggtta tattcaccgt tacagcgatt tacaaaatta agtcattcgc tcactgacgc
    28561 caattaaata ttcctgtatc gttcttaacg agcagtctgc ttatattcat ataatcaatg
    28621 aatattaatt aatatataat acatagggaa tgtaaatgaa caaatttatt aaagttgcac
    28681 tggtaggtgc agtactggct acgttaactg catgtactgg tcatattgaa aaccgtgata
    28741 agaactgctc ttacgactac ctgctgcacc cggcaatttc tatttctaaa atcattggcg
    28801 gttgcggtcc tactgcacag taagtctctg acagaaatcg gctaacaccg gtttaattgc
    28861 ccgacgccct ctggattttc cgagggcgta tttgtttcta tgtccttctt gcccccgccc
    28921 gtaaaaatat tttcacttta aattcaattt tataactaca tccattagcc acagcgcgat
    28981 cctgtgcgaa attttgtgat cttcctccac atcacataac ataatatgtt gttatattca
    29041 tcatgcattg tcatgttacc ttttaaatgg ctgcaaactc tcccctacaa cgtattggac
    29101 aagaaaaagg tatcgctatg ggaagccagg aactccaacg caagctcgga ttttgggccg
    29161 ttcttgcaat cgccgtcggg acaaccgtcg gctccggtat ttttgtatct gtgggtgaag
    29221 tggcaaaagc agcgggcacg ccgtggctta cggtgctcgc gtttgtcatt ggcgggttaa
    29281 ttgtgatccc gcaaatgtgc gtctatgcgg aactatccac cgcttatccg gaaaatggcg
    29341 cagattatgt ttatctgaaa aatgccggaa gccgaccgct ggctttcctc tccggctggg
    29401 ccagcttctg ggccaacgat gcgccgtcat tgtcgattat ggcgctggcg attgtcagca
    29461 atcttggctt tttaacgcct atcgatccgt tgctcggtaa atttatcgcc gccggattaa
    29521 ttatcgcctt tatgttgcta cacctgcgct ccgttgaagg cggcgcagcg tttcagacgc
    29581 taattaccat cgccaaaatt atcccgttca ctatcgtcat tggccttggg atcttctggt
    29641 ttaaagcgga gaattttgcc gcccttacca ccactgcgat tggcgcaacg ggcagcttta
    29701 tggcgctgct ggcggggatc tctgccacca gttggtcgta taccggcatg gcctctatct
    29761 gttatatgac cggcgaaatt aaaaaccccg gaaaaaccat gccacgagcg ctgattggtt
    29821 cctgtctgct ggttctggtg ctctacaccc tgctggcgct ggtgatttcc ggcctgatgc
    29881 ccttcgacaa actcgccaat tctgaaacgc cgatttccga cgccctgacc tagatccccg
    29941 cactcggcag caccgctggg ctctttgttg ccatcacggc gatgatcgtc attcttggtt
    30001 cgctttccag ctgcgtgatg taccagccgc ggctggaata cgcgatggcg aaagacaacc
    30061 tgttctttaa atgcttcggc catgcgcatc cgaaatacaa cacgccggat gtctccatca
    30121 tcctgcaagg ggcgctgggg atcttcttca tcttcgtttc cgatctcacc agcctgctgg
    30181 gttatttcac cctggtgatg tgtttcaaaa ataccctcac cttcggctcc atcatctggt
    30241 gtcgtaaacg cgacgattac aaaccgctgt ggcgtactcc ggctttcggg ctgatgacca
    30301 ccctcgccat tgcgtcaagc ctcattctgg tcgcctcaac ctttgtctgg gcaccgattc
    30361 ccggccttat ctgcgccgcc atcgttattg ctactggtct gcctgcttac gccttctggg
    30421 cgaagcgtag ccgccagctc aacgctttgt cgtaatgtta tctgaagaaa ataaaatgtt
    30481 ggatattgat aaaagcaccg tggactttct ggtcaccgaa aatatggttc aggaagtgga
    30541 aaaagttctc agccatgacg ttccgctggt gcacgccatc gtggaagaga tggtgaagcg
    30601 cgacattgat cgtatttatt tcgttgcctg cggctcgcca ctcaacgcgg cgcaaacggc
    30661 gaaacatctg gcggatcgct tttccgatct tcaggtctac gccatttccg gctgggagtt
    30721 ctgcgataac accccgtatc gcctcgacga tcgttgcgcg gtgattggcg tttctgacta
    30781 cggtaaaacg gaagaggtaa tcaaagcgct ggagctgggc cgggcctgcg gagcactcac
    30841 tgcggcgttc accaaacgcg cggacagccc gattacctcg gcggcggaat ttagcattga
    30901 ttatcaggcc gactgtatct gggaaattca cctgctgctc tgctacagcg tggtgctgga
    30961 gatgatcacc cgcctcgcgc cgaacgcgga aatcggcaag atcaaaaacg atctcaagca
    31021 gttgccgaat gcgctcggtc atctggtacg cacctgggaa gaaaaaggcc gccagcttgg
    31081 tgaactggcc agccagtggc cgatgattta taccgttgct gcgggtccgc tgcgtccgct
    31141 gggttacaaa gaaggcattg taacgctgat ggaatttacc tggacgcacg gctgcgtgat
    31201 tgagagcgga gagttccgcc atggcccgct ggagattgtc gaaccgggcg ttccgttcct
    31261 gttcctgctc ggcaatgatg aaagtcgcca caccaccgaa cgcgccatta actttgttaa
    31321 acagcgtact gacaacgtga tcgtcatcga ttacgccgaa atttcgcaag ggctgcaccc
    31381 gtggctggca ccgttcctga tgttcgtgcc aatggagtgg ctctgctact acctgtctat
    31441 ttacaaagat cacaacccgg atgaacgccg ctattacggt ggtctggtgg aatattaatc
    31501 cctctcccgg cccggcaacg ggccggattt acgcaaggag ttacccgatg aaaacaggta
    31561 tgtttacctg cggccaccag cggctgccta ttgaacacgc atttcgtgat gcaagcgagc
    31621 tgggttacga cggcatcgaa atttggggcg gtcgcccgca cgcgttcgcg ccggacttaa
    31681 aagcgggcgg catcaaacaa atcaaggcgc tggcgcagac gtatcagatg ccgattatcg
    31741 gttacacgcc agaaaccaac ggctatccgt ataacatgat gctgggcgat gaacatatgc
    31801 gtcgtgaaag cctggacatg atcaagctgg cgatagatat ggcaaaagag atgaacgcgg
    31861 gttatacgct gatttctgcg gcccacgcgg gctatctcac gccgcctaac gttatctggg
    31921 ggcggctggc agagaactta agcgaactgt gtgagtacgc ggaaaacatc ggcatggatc
    31981 tgatcctcga accgttaacg ccgtatgaat cgaacgtcgt gtgtaatgct aatgatgtgc
    32041 ttcatgcgct ggcgctggtg ccttcgccgc gcctgttcag catggtcgac atttgcgcgc
    32101 cgtatgtcca ggcggaaccg gtgatgagtt atttcgacaa actgggcgat aaattacgtc
    32161 atctgcatat tgtcgacagc gacggggcca gcgacacgca ttacattcct ggagaaggca
    32221 aaatgccgct gcgggaactg atgcgcgata ttattgatcg gggctatgag ggttactcta
    32281 cggtggagct ggtgacgatg tatatgaacg agcccagact ctatgcccgc caggcgctgg
    32341 aacgctttcg cgcgctgctg ccggaggatg agagatgaaa accctggcga caatcggcga
    32401 taactgcgtc gatatctacc cgcaactgaa taaagcgttt tctggcggta atgcggtcaa
    32461 tgtggcggtg tactgcactc gctacggcat acagccggga tgcattacct gggtgggtga
    32521 cgatgactac ggcacaaagc tgaagcagga tctcgcccgc atgggcgtcg atatcagcca
    32581 tgtccatacg aaacacggcg ttaccgcaca aactcaggtg gaactgcacg acaatgatcg
    32641 cgtttttggc gactacaccg aaggcgtgat ggccgacttt gccctgagtg aagaggatta
    32701 cgcctggctg gcgcagtatg acattgtgca cgcggcaatc tggggacatg cggaagacgc
    32761 attcccacag ctgcacgctg cgggcaaact taccgctttc gacttctccg acaagtggga
    32821 cagcccgctc tggcagacac tggtgccgca tctcgatttt gcctttgcct ccgcaccgca
    32881 agaagacgaa gcgctgcgtc tgaagatgaa agcgattgtt gcccgtggcg caggaacagt
    32941 gattgtcacg ctgggtgaaa acggcagcat tgcctgggat ggcgcgcagt tctggcgtca
    33001 ggctcctgaa ccggtgacgg ttatcgacac catgggtgcc ggagattcgt tcattgccgg
    33061 attcctttgc ggctggtctg cggggatgac attaccgcag gcgatggcgc agggaacggc
    33121 gtgcgcggcg aaaaccattc agtaccacgg tgcctggtag gtataacgtt ggcgtgagca
    33181 tcttcacgcc aacgtgctgt tacttgccgg aaaacgaccc tataatccga gtaattcatt
    33241 ctttatttca gggtcgatta tgtcagctac ggaccgctac tctcatcaac tcctctacgc
    33301 taccgtccgc cagcggctgc tggatgatat cgcgcagggg gtttaccagg ccgggcaaca
    33361 gatccctacc gaaaacgagc tttgtacaca atataacgtc agccgcatta ccattcgcaa
    33421 agccatcagc gacttagtgg cagacggcgt actgatccgc tggcagggaa aaggcacctt
    33481 tgtacaaagc cagaaagttg aaaacgccct gcttactgtc agtggtttta ccgattttgg
    33541 cgtctcacaa ggcaagtcga cgaaagagaa agtgatcgaa caggaacggg tcagcgccgc
    33601 gccgttttgc gaaaagctga acatccccgg aaacagcgaa gtgttccatc tctgccgggt
    33661 gatgtatctc gataaagagc cgctgtttat tgatagttca tggatcccgc tgtcgcgtta
    33721 tcctgacttt gatgagattt acgtcgaagg aagctccacc tatcagttat ttcaggagcg
    33781 ttttgacacg cgagtggtca gcgacaaaaa gaccatcgat atctttgccg ccacccgccc
    33841 gcaggcaaaa tggctgaaat gcgaactggg cgaaccgttg tttcgcatca gcaaaatcgc
    33901 ctttgatcag aatgacaaac cggtgcacgt ctccgaactc ttctgccgcg ccaatcgcat
    33961 caccttaacg attgataata aaagacatta accgtaggcc ggataagatg cgccagcatc
    34021 gcatccggcg atgctggcgc actgaatttt acatcccgca cgttcccctc accctaaccc
    34081 tctccccaaa ggggcgaggg gaccgttcag tgcggaattt agcttgattg ccgaacggtg
    34141 ctatccagca ttaaatactc gcaatactct cacgcaaaat ccgcagcacc gtctcttcac
    34201 cgctgcgatt cgggttaatg cgaatcgcac aatgttcgag ttgtggattc gcctggcgaa
    34261 acgttcctga aagtcgatag aagagcggcg ggatttcata tttcgactct gcaccgaccg
    34321 ggtaaggcaa cgcgccacgc ttttgcgctt cttccagcac cctggcggca atcagctgat
    34381 gaaactcgac aatcaacacc ttcgactgcg cattggcaat gacggcgctt ttcacttccg
    34441 ccaccgcgcc gccgttaagc aaagccagca accgttcgga tactcccgcc tgcaccgcat
    34501 gcatcactgg cgcgaacacc agaccacgca agacttccag cgcctgtgag ccctggatct
    34561 ggctaccgcc ggagtaaagc gtggcgcgaa tccgactaat aacatcggca tcgccaacca
    34621 ctgcaccaac gccctccggc ccaaacagct tgaagcagga gaatgtcgag acattcgcgc
    34681 cgcattcaca gccgattcgc gctaccttca tcaccgcata gttatcatcg gttaacgctg
    34741 gaacacctgc cgcgcgcaac gttgccagca catctgccag cacatagctg tcctgcggct
    34801 gctggcgcgt atgctgcacc agcgcggcat caggttgttg ctcgtcgacg acctgcttca
    34861 gtgccgacag gtcattgaaa tcagcagtaa taagcgtcag ccccatctgc tcaataataa
    34921 cccgtgtcgt cgggtaaaca ggcgcgtcat gcaccagaag acgctgcccc ggtttgagca
    34981 aagccgccag cccggcgcga atcgcgccag tccccgcgcc ctgcaccagc gccgcggcct
    35041 gtgcgtgaaa tgcatcagcc agcacctgct ccacccgttg ggtaatacgc ggttgattca
    35101 gacttggagt taaccctaaa tcaccgccag caagaaactc gctgcctgga aaatggcggc
    35161 aaatagtgtc caccagcgca aactgctttt gctgcgcttc agcaagagtc aggctttgca
    35221 aaggaaacgt cttcataggt gccttcctta agccggaacg aacaggccaa gccagtaaag
    35281 aacattcagc agaataccgg tgatcattac tgccacgacc ggtgccgcca ttttctgtac
    35341 cggacggccc agggattcgt tgaggaagta aatggcaacc gcgatagaga atccggtata
    35401 acccgccatc ttaattgctg cgaaaatcga accgaccagc agcgccactt ccatcagcat
    35461 attcatggcg ttacggatgt tatccgacgc attacgcacc gacgggtagc gtcccagcca
    35521 tttgccgatc gaacgaagca gcaagacttc cgccgaaata accactgcgc ctaataccgc
    35581 tgcaaccatc ggattcggcg agagatagcc caccgcataa acaaaggtaa agcccgcaac
    35641 tgcatacaca cccgttgcta aggcggtggt ggcaatcaac ggcacaaacc ccagtccgcg
    35701 cataaattct gccagagccg cctgattaat cagcgtttgc gattgttccg gcgtcacgcc
    35761 tgcggaatac gctttctcca gtgtgaagat cgaaacttcg ctaccagcaa aaatcttcat
    35821 actggcaacg gcggcaatca atgctcccac gatggcgata taaggtaagt ttttgataat
    35881 ccgcgacgtg cgctcttcaa acaccgaaag cccgctggca tcgatgtcat tttcatcacg
    35941 atgacgcagg tcgtgagtta tcgcgatccc cagcagcatc accatgccaa taaagatttc
    36001 gatggattca gggttaagat gtgggaaata gcgcacgact accacacggg tcatcagaac
    36061 aaccacggcg gcgaccagac tttgcttcca gccaaactgg tagaaaatcg ccaccagcgg
    36121 gaacagtgca aaagcagaaa ccaccggcga gcttaattcc ccgaggctac ctaatacatc
    36181 caccggcagc gcggtcagca gctggttaac tggcaacagg caagtaagga tcaacacgcc
    36241 ccagatagcg ccaagaccaa acgccatcag gctgtttatc gccagtacgc cgaggatatc
    36301 ggtaggaaga aagagtaacc atgcgttgag cagcccggtt ttcagggtaa aagagatccc
    36361 caccgaggcc acaaaaccaa tgctcagacc aaaagcgata ctccccgcct cgcgacggtt
    36421 catatagcct tcaatcagtt gcggcaggat cgggtggatg ccgtcatgaa aaacagccgc
    36481 cgagcgatgc tccagaagcg atgtcatacc cgtcaggcac gccaccacga taatctgaat
    36541 atacagatcc atagtcgtgc cttttatttc agatggttaa tcaacatcgg aatcgcgtgt
    36601 tcgacgtgct caacggaaag gccaaacgcc actttccctt cagcgatcat tttggcgata
    36661 tgctcgtctt tcgctttaat gcctggtttg gcaatggtgc aacttttgtt atagccaatc
    36721 accgcgatgg caatcgacaa tgccgcgtcc gcaccggtat tacacgcgcc aatgtaataa
    36781 tctagttgcc cggatttcac cttcattgcc gcttccatgt cgttgtgaat gaaaacttca
    36841 aaacagccag gagccgtcgc ttcaatggtt tttttaatct gctcacgctg taagcctgca
    36901 acgccaatct ttttcatgag tctgtcctta ttggaaaaat tgagagggat tttcacgtaa
    36961 catcacatcc acatcggcct gactgaatcc tgactggcgc aattgcggaa taaaggtggt
    37021 caataaatag tcatagccat aaccaccatt ggcttttaaa tgggagcggc gcgtaatatc
    37081 catcgacagc atgacgcggt tcagcaaccc acggtcacgt agcgcgtgga gcatcgcaat
    37141 gcgcttttcg tccgggtagt aactgttctt accaatggtg tcgaactgca cgtacgcgcc
    37201 gagatcgatc atcttcaaaa tgttgtcgag gttgtctttc agatcgcagt gaccaacggt
    37261 gacgcgcgaa agatcaactc cgtgggcttg caacagagcc agttgttcca gccccatggt
    37321 gctgaacgac gtatgcgtgg agatcgggcg tccggtctgg ttatgcgcca gcgcagcggc
    37381 aataaatacc ttctcttcca gcggcgtaat ctttccttcg ctggagccga tctccgcgat
    37441 gatcccggct ttcagctccg tgccatcgat accctgttca atttcatcga ccatctcctg
    37501 cgccagttcc tgcacgctgc gggtcaccac atgttccggg aaaaacgcat cctggtaata
    37561 accagtacag gccaccacgt tgatcccggt ttcgcgcatt acatcgagca taaattgcgc
    37621 attgcgcccc atgtaacggt tggtcatctc aatcacatta cgtacacccc gggtcatcag
    37681 gtcgttcatc tcctggcaaa tgaacgcata ctgatcaagg cggcagtcca cgttgttttt
    37741 aaagccggag agatcaatat gcagatgctc atgggccagg gtgtaacccg tcggatcaaa
    37801 actcataggg tgtctcctgc aaaccggagg gaggaaagaa aagagcgacc attttgtggc
    37861 ggtggcgcac ggaaaaattc acacacggta gctcccacat cagaaagcgt ggtgcgcaca
    37921 ccgagctgcg tggcgaccag cccttgctga taaaccagca ctggcaccac ttcacgggta
    37981 tggtggctgt gaccaatggt cggatcgtta ccgtgatccg ccatcacgac caggcaatca
    38041 tctggctgca tcgcctcaac aagccgggca aggttacggt caacgacctg caaacgttcg
    38101 gcataacgtg cgacgtcttc tgcatgacca gcgaggtcgg tttcctggat attgatgcaa
    38161 ataaacgccg tcggatgggt gttaaattcg tcgagggtga tatccataat ccgctggcta
    38221 tccaccaggt tttgccagct cacaccataa ggattgctga cgatatctgc caccttaccc
    38281 accagcacgg ttggcacgcc cgcttcatac agtttttgcg gcacctgcac tttttcatca
    38341 acaccgtagc ccatatgcac gacctggaag ccgttgtcat aagcgccaga acgcggcgca
    38401 ttaataccaa taaagcgccc ttctttgctt tctgcggcat cgagaatacg ctgactgttg
    38461 gttaacaggc cgccaaatgt aatgacccga ccgacctgta cctggtcacg cacgatacga
    38521 ccaattttga ttgcgtcgtc aaaagagatc acagagagat tggcggtaat gttatagact
    38581 tggcctaaat ccgcctcgag attatcgcca atcgcaaccg cctgattgac cgacagaaat
    38641 tgcagatcat cgccacggcg ctccacctgc cagccagcgg aaactaatgc ctgctcaaca
    38701 cggtcaatca catcgcgaaa aggcatccgc agcggcggta acgggcgcgt gcctaaaatt
    38761 tcctgatgcc ccataaaggt atcgccacct tcatgttgca gctctgccac gccccaggtt
    38821 gcggaatctg acggctgcat atcgcctggc gcataaccca atgtgttgat tagccccagc
    38881 ttctccagcg ttggtagctg caaatgcggc aactggctca ggatgtgacc acaggtattc
    38941 gccccggcat cttgcgggcg caccagcgtg acatctttca ttgcccctac gccaaagcta
    39001 tcaatcacta acaccacaaa tcgcgccatt tatgcctcca gagggttgcc gaggctgtca
    39061 taacgaccaa ctatttctgg ttcgccgcgc tgaataccgg acaccagcac cacatcgcta
    39121 cgggtgacaa aaatctgcgt gcgaaaacag agcaccactg cactgctcac cggatactcg
    39181 cctgccagcg gcagggtgta gtcgatactg ctgtcatcca cagttttgag attggtttca
    39241 gtaatctttt gattttctgg cgtaaacacc agcgcatgtt gcgcatgacc acgacggtag
    39301 taaccgccgc cgtagcagta gctgtcgcca cggaaatgat gggagatttc acttaaccag
    39361 agcatcgcga tacgttcagg ctgatcgccc tgctggtttg ccggaatagt accggtcagc
    39421 gcatgaccgg gttcggcatg agtcgcaccg tattccgcca gcaatggcag cgaagtgcag
    39481 ctggtcgctg aaggtacgtt cagttgctca atcgcaatac cagatttcgc cagttgatcc
    39541 cgtgcttgta ccagcgtgtg aagattcggt gttggcaaaa ctttcccagc agcctcatcc
    39601 caaagcaggc aagggaaatg ggttagtccg gctaaatgca gccccggcag tttcttaatt
    39661 tcagcaatca cctcgtgcag cgaatgctgt acaaaaccgc tctcctgccc cggataaaga
    39721 aaatcatcgt cgctataaac tttgagcagc acaggctgga cccgtccggc cttaaccgct
    39781 gccgcagaaa cttcccgcgc tttgtcgaga gtaaacacgg tgatgatgtc ggtgccctgt
    39841 tcaacggcgt cagcaacctg atgacaaggg atttgtacca gatgcccctg atgcgccact
    39901 ggcaaaccag cgcggcgcat gactcgcgcc tctttgtaat ccaccgccac aatgccgctg
    39961 tagcctaatg ccagcaattt ttccgccagc cacggattac gaccaaattg cttggtcatc
    40021 agatacagtt caatgccgta aagccgcgcc gtctcaatga gccgcttacc gttttccagt
    40081 atctgatcca cgtcgatcac ccagctgtcc ggggcgattt tgccctgctg ccacaaactt
    40141 agtgcggcgg aaatcagcgc cgggttctgg cgtttcaatg cttctacaaa catcttgttg
    40201 cctcgtttct gttccttgaa ttaaatattc atttttttga atatttaggt tgtgcgacgt
    40261 ggatttgtcg ggagcttcaa acctcttcgt tagcagccat ccataatcca taaagattgg
    40321 ccagaaaata gccttcttca cacgggttaa cttccagagc gaattccttc aacaactcct
    40381 gatgcaattg cacaacggct tgccagtggc tggattgcgc cagttccgcc agcaactcgt
    40441 tatccagcgg ctctatttct tcaccacggc gactgcgcat cagtgcactc gccatatgtg
    40501 tcatcgccat cgttccttgc tcactgcgca ccggcagatg gcactctttt tccagtacgt
    40561 tgacgacctg catcatgcct ttgcagacgt ccttatcaat aacgcctgcc tcgcaaagca
    40621 ggttgagtct ggtttccata tcagatgcct ttcgtttaat aactgggttc ctgttcccca
    40681 ctcaccaccc gttgttgatg ggcaagcagg gcgtgtttat ctacaacagc acgcagcaat
    40741 tgttgcattg ggtaatcatc gactcgcgtc aggaccacgg cttcggtggc ctgtaaaaat
    40801 cgcggatcgt ctgttagcgg cgtcgcctct aatcccagca tggtcagttc gttttccgcc
    40861 accacgttcc agatcaccgc atcgacatcg cctttaacaa tgcgttgtaa actctcgtga
    40921 taagagagat cgactcgttc cacatcacta tcgccaaaaa agacatcggt catgattttc
    40981 tgatccgccg aacgactatc gagacctact cgcttaacat ttccagactc acctttgcgg
    41041 catatcagtt gatgttcacc aacatacgta tgcgggccaa gctcgagggc gatgcacaaa
    41101 ttgttttggc tgagataact ttccgccgcc aggcgggaaa ctactgccat gtcatagaca
    41161 ccgttaagta aacactccac gcgaatatcc gcgccgcgca tatgcgcata gtaaaaagga
    41221 atgccatcaa actgggcttt caatccgctc gctaaacctt cgtacaagcg ggtatagggc
    41281 aaaggcatcg cacataccac gttgttgata tccacatgac tcagcaatgc tttgttatcc
    41341 atctcgacaa gataactgcc attgcgccca cggcgttcaa tccgtatcgc tccgcttgat
    41401 tccagcgttt tcaacgcggc ctgcgtcagg ccaaccgatg aacggcattc agttgccagc
    41461 tcatctatgg ttttcagtcg attaccgcat ttttcaccca acaaataacg ggccagtgtc
    41521 gtgatgacga cgccttcttt tttgataaac gttcgacgca taatatattt tcaataaaat
    41581 gaatatttat atcttcatta aaatgaagat aagtggcgac gacgagatgt ggaaacggcg
    41641 aggcgcttca cattttttcg ggttgttgcg aaaagaggat aaaaaaaccg ggtttccccg
    41701 gtttcagagt gatgacgaag tgatttattc gacaatgcga gcaaagggta attcccactg
    41761 gcgcttgcct gctcccctca ccctaaccct ctccccaaag gggcgagggg accgtccgag
    41821 cgttttgata ttacggcttc gccacaaaac caatcgcttc gtacaccgct ttcagcgtac
    41881 gggaagcgtg tacactggct ttttctgcgc cgtctttcat cacctgttgc aggaaggctt
    41941 catcgttgcg gaaacggtga tagcgttcct gcaattcagt cagcataccg gaaacggcat
    42001 cagccacttc acctttcaga tgaccataca tcttgccttc gaactgtttt tccagttctg
    42061 ggatgctctg gcccgttacc gcagaaagga tatccagcag gttggaaacg cccgctttgt
    42121 tctgcacatc gtagcgaact accggcggct cgtcggagtc agtgaccgcg cgtttgattt
    42181 tcttcactac cgatttcgga tcttccagca ggccgataac gttattgcgg ttatcgtcag
    42241 acttggacat cttcttggtc ggctccagca gcgacattac gcgcgcgcca gatttcggaa
    42301 taaacggctc cggcacctta aagatctcgc catacagcgc gttgaaacgc tgggcgatat
    42361 cgcgactcag ttccaggtgc tgtttctggt cttcacccac cggtaccaga ttagtttgat
    42421 acagcaggat gtccgcagcc atcagcaccg gatagccaaa cagaccagcg ttgatgttct
    42481 cggcataacg cgcagattta tctttaaact gcgtcatgcg actcagttcg ccgaagtagg
    42541 tatagcagtt cagtgcccag cctaactgcg catgttctgg tacgtgggac tgaacaaaaa
    42601 tggtgctttt ctcaggatcg ataccacaag ccagatacaa ggccagcgta tccaacgtcg
    42661 ctttacgcag cttctgtgca tcctggcgca cggtgatcgc gtgttggtca acgatacagt
    42721 aaatgcaatg gtagtcatcc tgcatgttta cccactgacg cagcgcaccc atgtagttac
    42781 caatggtcaa ttcacctgag ggctgtgcgc cactaaaaac gatgggctta gtcatttttc
    42841 gattcctgat tttcgctatg cggaagccct aatgcgggca gaaggtcatt tatagactga
    42901 taaattacat caggctggct gagatcgata gcctcgccgt agttgtatcc gtaggttaag
    42961 ccaactgatg ggcaacctgc cgcttttgcc gcctgaatat cattccgtga gtcgccgaca
    43021 aacagcatct gttgtggggc aattcccatc cgctcagcca ccagtaacag cgggtccgga
    43081 tgcggttttt tgttttgcac atcatcgccg ccgataacca cgctaaagta tttggcaata
    43141 tctaaggctt cgagcagcgg cgcgacgaac ggcgtcggtt tgttggtgac caagcctagc
    43201 ggcaggcctt tagcctgcaa cgcgcccaac gtatcggcaa cgtgcgggaa caaaaacgtc
    43261 ccctcttcgg caacctcgcc atagtagcga tcgaacagtt tacgcagaat acgtacctgt
    43321 tcttctgccg gaatgtcgtc atcaacgggc ggtttcccca ttgttttacg cagagtcgca
    43381 cgttcctgac gcgcccaggt caatgcgcgc tccatcagaa catctgcgcc gttaccaatc
    43441 caggtaataa cgcgttcttc acctgcgacg ggcaactcca gcgcatacag cgccatatct
    43501 accgcagcag caagaccagg agcactgtcg accagcgtac catcaagatc aaaagcgacg
    43561 ccgcgaatat cttcaaactt attcatgact tacctttgcc agttcactgc gcatttcatc
    43621 aatgactttt ttatagtctg gctggtcgaa gattgccgaa ccggcgacga acatatccgc
    43681 gcccgccgca gcgatttcgc caatgttgtt caccttcacg ccaccgtcca cttccagacg
    43741 aatgtcaaag ccagactcgt cgatacggcg acgtacttcg cgcagtttat ccagtgtttg
    43801 aggaatgaaa gactgaccgc cgaaaccagg gttgacggac atcagcagga tcacatccag
    43861 cttatccatc acgtaatcca gatagctcag aggtgtcgcc gggttaaata ccagacccgc
    43921 tttacagcca ttttctttaa tcagttgcag cgtgcggtca acatgctcgg aggcttctgg
    43981 atgaaaggta atgatgctgg caccagcggc agcgaaatca ggcacaatgc gatcgacggg
    44041 tttcaccatc agatgtacgt cgataggggc ggtaatgccg tagttacgca aggatttcag
    44101 caccattggc ccaatcgtca gattgggaac atagtggtta tccatgacgt caaaatgcac
    44161 gacatcagcg ccagctgcca gggcttttgc ggtatcttca cccaggcggg caaaatcagc
    44221 cgacagaatt gagggggcaa tcaaatactg tttcatccgc ttctccttga gaattatttt
    44281 ttcgcgggtg aaacgactcc tggtttgtac aaagccagca gttcgtccac ctttttacgt
    44341 gtgccgccgt tgctgcttat actgcgtcga actttgacga catgcaattt tgcgcgctga
    44401 taccactcac gcgttaacat cgtatcgtga ttggagatca gcactggaat atggcgctca
    44461 accagacctt cggcgatctc cgccagatgc gcttgttgtt caagcgtaaa actgtttgtg
    44521 tgatacgccg taaagttggc ggtcgcagac agcggtgcat aaggcggatc gcaatagacg
    44581 acggatgcat catctgcgcg ctccatgcta tcggcgtaag actcacaata gaaaaaggca
    44641 ttctgcgctt tttcagcgaa gtgatacaac tctgcttccg ggaaataggg ttttttgtag
    44701 cggccaaacg gcacgttaaa ctcaccgcgc agattgtaac gacacaggcc gttgtaaccg
    44761 tagcggttca aatataaaaa cagtaccgcc cgacggaacg gatcctggct tttgttgaac
    44821 tcttcgcgga actgatagta aacctcggcg caatttgttt cgggaacaaa cagctcgcgt
    44881 gcggcctgta cgtactcatc agtacgcatc ttcacaatgt tatagagact gatcaggtcg
    44941 ctattgatat cggcaaggat ataacgagaa aagtcggtgt tgagaaacac cgacccggca
    45001 cctacaaaag gctcaaccag acattcgccc ttgggcaaat gccgtttaat atcatcaagc
    45061 aggggatact tgccccctgc ccacttcaaa aaagcgcgat ttttcttcat gccgattaac
    45121 taattacacc ttctccggct gtggagaaag ctccgacagc atccagcgct tcaggcagct
    45181 cccgcaacga taaaccattg cgggagataa ccttgattac ttcagatcgg cctgtacctg
    45241 acgcagcggt ttcgcccacg ggtttttggc ctgaacatct gctggcaatg tagataccgc
    45301 ttttttcgcc tcttctttcg aagcgtacac gccagaaacc aggacatacc acggctgacc
    45361 attacgcgtc gtttcataga caacgtagtt tttcagattc tctttcttcg cccaaccgtt
    45421 caggttgtcg tagttagagg aactgctcag ctgcagagtg taatggctgg acggtgccga
    45481 tttcaacgaa ccaacattac ctgcggtctt ccctccagcg gctggtgttg ccgtggtttg
    45541 cgccggggat gccgtctgta ctggagccgt agtcgccgtc tcttttggtg ctggcgtaga
    45601 agtcgcagcc ggtgccttcg tgctcgccac aggggcagct ggttcaggtt cagtacgctt
    45661 cggcgtctgt gctaccggct tcggctccgt tttcacggtt gcttgcggtt ttttcggttc
    45721 aatcaccgcc tgctgacgaa ctgggcgcgt agtggacgga cgttcagcgg tttgcgtttt
    45781 cgccgtgtca cgcgatgcat tgccattgcg aacaggcgca accgttgcgg gttcagtcgg
    45841 caacgtggaa ttgaccgcca cattgttcag ctgttgctga ttttgtggct gggtcagcgc
    45901 attgttcagg tcaccctgca cttcaacacg ttgttgacca tccgttgcca ccggggtttg
    45961 cccttgagtc ggcgtagaag agatcggtgg cagagaaaca tcctgctgag tattttccgc
    46021 agacgtggtt cccggcgctg gctgcacgcc attcgcctga tcggtcgcat tgccagcaag
    46081 atcaatactc ttctcgccag acgcggtttg atcgctggaa gtggtcgagg gggcttttag
    46141 cgcagaaccg ataccgatga tcaacagcag tagaaccaga atgccgacgc ccatcatcat
    46201 atactgacga gaagcgggtt tactggctgc ttttttgcgc ttacgcggac gacgctctac
    46261 gcgctcttca tccacggttt catcttcgga ttcgtcaatt tcttcttcga tttccggttc
    46321 ctcattgcgc tctttttgcg cacgagtcgg acggcgatcg tcagtgtcat caagttcaat
    46381 atcatcaaaa ttgatctgcg gttcgccacg ttcagtacgc tcagaacgtt cagaagattg
    46441 acgagaacga ccagtacgac gatcgctggg atcgggtttc agctcgtctt ctggtttgaa
    46501 ttcatccatt taacacccca ctaaaaggtt aatgcttacc acgttgcaat taacctgaag
    46561 ctaatagacc gcttgataag cggcctgacc tttcttgttg ttacgctgat tgacaatcgg
    46621 caatggcgtt aagaacaagc tcgtgcgaaa cgccgctgcg aacttcactc ttaccaattg
    46681 ccaacggaag aattaagcgc atctctcccg caaggacttt cttgtcacgc agcatatgcg
    46741 gtaaatacgc ctgcgcggac atttcgcgcg gcccattgac cggtaaccca gcccgcgtga
    46801 gcagggttat aatacgctgc gtttcggcag aactaaactg cccgagacgt tccgacgtcc
    46861 gcgccgccat caccataccc gcagcgaccg cttcaccatg taaccaattg ccatacccca
    46921 tttcagcttc aatggcatga ccaaaggtgt gtcccagatt cagtaaagca cgtaacccgg
    46981 tttcgcgctc gtcggcggcg acaacttctg ccttcagttc acaacaacgg cgaatacagt
    47041 acgccattgc cggaccgtcc agacgcaaca acgcatccag attctcttcc agccagttaa
    47101 aaaacgcacc gtcaagaata atgccgtatt tgatgacttc tgccagcccc gacgctaact
    47161 cacgcggggg aagcgttttc agacagtcga gatccaccac cactgaagca ggctggtaga
    47221 acgcgccaat catgttttta ccgaggggat ggttgaccgc agttttgccg ccaacggagg
    47281 aatcgacctg cgacagtaac gtcgtcggga cttgaatgaa acgaacaccg cgctgataac
    47341 tcgccgccgc gaagccggtc agatcgccca ctacgccgcc gccaagcgcc accagcgtag
    47401 tatcgcgacc atgcggcttt tgtaacaacg ccgtaaagac ggtatcgagt acagctaggc
    47461 ttttatactg ctcgccgtca gggaggataa cgctatcgac gttaacaccc gcctgttcaa
    47521 gtacgccgcg gaccttatcg agatacagag gagccagggt ttcgctggtg accaacatga
    47581 cctgctcgcc cgatttcagc ggtaagaatg aagctggttc attaaacaaa ccagatgcga
    47641 tggtaattgg gtaactacgt tccccgagag taacgacaat cctctccata acgcgacatc
    47701 caccttaatt actgtacccg cagacgagtg tatataaagc cagaattagt tgctttccag
    47761 catgtgaata atctggtttg caaccacttt agcgctttga tcatcagtac gaatggtcac
    47821 gtcggcaatc tcttcataca gcggattgcg ttcattggcc aacgcttcca gaacttcacg
    47881 cggcggtgtt tcaacgtgca gcaacgggcg ttttttatca cgctgcgtgc gtgcaagttg
    47941 cttttcgatg gtcgtttcaa gataaacgac aacgccacga gcggaaagac ggttacgcgt
    48001 ttcacgggat ttcacagagc cgccgccagt agccagcaca ataccctgtt tctcggtcaa
    48061 ctcattgatg accttttctt cgcgatcgcg gaagccttct tcgccttcta aatcgaaaac
    48121 ccagcccaca tcagctccgg ttcgtttctc aatctcttga tcggaatcgt aaaattccat
    48181 attgagttgt tgagctaact ggcgcccaat agtgcttttt ccggcaccca taggcccaac
    48241 cagaaagata ttgcgtttct ctgccatttt ttcggtacta ctaagactat tcgttaatga
    48301 taaacccgct tcgctcagag agcgccgcag gacatgaact gaaacctcat aagatattgc
    48361 gagagtcaga ctgaaaatta tctcaatact ccagcgggtt tggcaactga ataaatcacc
    48421 catccctctg catatctggt cgctgcaagc gcgctgcctt gctaccaccg ctctggcgat
    48481 aaatcaccgc cgggtaagat tagcgtaaaa aagacagcaa aatgccgcct gaatgataaa
    48541 tcatcatcat ggaacgcagg cagcgaagag caccaactct caaatcggta ctccttgtat
    48601 gctaaatacc tgcgcgcgtc aaatagatga aacacgttca gcataaaaac attaccgctt
    48661 ttacggctgt ttactcactg gaaaccagtc gtggcgtgat aaacaccact aactcgcgtc
    48721 gttcatcttc ttttccgtca tgacgaaata attgcccgaa ccagggaatg tcgccaagca
    48781 acggtacgct atcctgaccc gatttatttt tacgggtaaa aatgccgccc agcgccaacg
    48841 tttctccgct ttttacctcg acctgcgttt cgatctcctg cttatcaatc gccagcactt
    48901 cgccatcggc ctgctgtagc acctgccccg gaacgttctg gctgatgtgt aatttcagcc
    48961 ggatgcgacc tttttgtaac accgtgggcg tgacctccat ccccaggacg gcctctttaa
    49021 attccaccga cgtcgcgcca ctttccccgc tggaaacctg atatggaatt tcgctctcct
    49081 gtttaatgct ggcaggctga agatgtgagg ccagcagacg cggactggcg ataatatcca
    49141 gctgctgttt ttgttcgagc gcggaaagct caagatccag caagcgtccg ttgatgcgcc
    49201 caatgttaaa accgacatgc gttgtcgccg tcgctacgga gaggtcgcta ccaagcgtgg
    49261 tgacttgccc aacgccacca gcgtgttgcg catcggccag cgtccatttc acgcctaact
    49321 cacgcaaact tttttcatta atggtgacaa tatgcgccga cagctcaacc tgcccgaccg
    49381 gcagatccat ttgcgctacc cactgttcaa gcgcgcttaa cgccgttttg ttatctcgta
    49441 gcaaaaggcg attggtgcgt ttatcgacgg tcatactccc tttggcactc agtagcttct
    49501 cccccgcttt cgccagttct cccgcgtcgg cgtattgcag ggttatactg cgattttcca
    49561 gcggcagatt tgcctgcgcc cgcgcctgct ccgcctcctg gcgggcgata ttgtcattct
    49621 gccaggcaat ggaatgcact gagagaatgt tgccttcctg ccgcgttatc agtccggcgc
    49681 ttttcactac agtttgtagt gcctgcttcc agggaacatc tgttagatgt aacgacaccg
    49741 taccgctgac gtctggcgac acgaccaggt tcaacttctc ctgttcagcc agcgcctgca
    49801 acacctgagc taccggaacg tcatccacca tcagcgtcac tttttgcggc tttgccgcct
    49861 gtacgccggg tatcagcatc aacagtagtg cggctatcca ttgcttcatt tgtatctcct
    49921 tgccgttgcc acaaccattg tggcggttcg cagtttgtcc cggtacccag cgttagtacg
    49981 tctggcgtca gctgtaaaat tgtccagccg ttttccagca catcgttttg ctgcacccgt
    50041 cgccatttct tttgcccgtc ttttattaca ccgatgatgc gctcgcctcg ccctaccatc
    50101 ccctgatagc gccactggct aagttcgcta atccggcata tagatcttcc ggcgatttaa
    50161 aagggtcacg cataccggtt aaaaggcaca atgcaatacc tgccaacaac cagcgtttaa
    50221 ccctcatgcg gcatctccag ttgtagcgtg aacaaaagat catcaccttc cacgcttaac
    50281 gaaaaacggc tcacgctgac gttgcgctct gccagccgtg taaatgccga cggcactgct
    50341 tcccacagcg ttttcaacgc caactcacct ccctgcgcgg atggatgcca ggaaaccagt
    50401 tgcgcgccgg ataactgaaa atccagtggc gaaaagggca gcgttttttc ctcgctgaag
    50461 ggagtggtgt ctaccaggcg atacagtgcg gcccactgct ggtgatgact tgcccgtagt
    50521 cgaattaatg cgtcgcgctc ttcatggtgt gtcgatgata gaaaaatgag cgtaactaac
    50581 atcagcaacc agaatgccca gcaaaactgg cggaggcggg gtgatgtggc gaaccaccag
    50641 tcaaagaaca tgttcatcgc taaccttcct tgttaactga tactcaaatt gccagcgtcc
    50701 ctgcgcatcc tgctgcgtgg ctccccgctg attgagatga aaagacgcat cctggcggag
    50761 tgacgtttct agtgcgttta acgcggtaat gcttgttgtc agccccttga tctccagcgt
    50821 tccctgctgc cagcttatcg ttgtcagcca ggcgtgctcg ggtaaaagcg ccgccagtgc
    50881 ttccagcgca gattgccagt cgcgggtaaa ttgtcgctgg cgctgccgtt gcgaacgctg
    50941 ttcgcgtaac tgctgttgct ccagcaaacg tggcttcgtt atctgtaagc tgcgggcgag
    51001 ttgttgttcc gcctgaagca aaactgcgtc tatgcgcgct tcggcgctgc ctgtcagacg
    51061 cagtattagc gttatcccga cggccagcag cagaggcgca acgaacatca gcaaccagaa
    51121 acgcagaaaa gcggtccggc gttgctgtcg ccagggcaaa aaattaattg gcggattcat
    51181 cagtacgcct tcccaagcgc cagccccagc gcgatggcaa agtctccacc aggcggcggt
    51241 agcggcggct gacgaacaga aacggcctcc caggggtcaa atccgccttc gccacatatc
    51301 gcgacgctgt cgggatcaac ggataacgct gccgccagct ccttcgcgct agtcatcccc
    51361 accgccagtt tgcgccccca gctatagcgc gtcgcccaca gccactgttc gttatcacgc
    51421 caggccagac attgctgatg agaaggtaaa aaaggcagga atcgctgtaa tgcactggca
    51481 tccggggtga tcgcactaac atgaacacgc aacctttctg ccagcgtaag cagcgttgcc
    51541 agctctttgc tttgcgcggc agtcacgtta taagcggggc tgagtgagtc ttcgctgtaa
    51601 tcgaagcgca gggagtccgg atccatatcc agctcgcggg ccatcgtccc tgacagccag
    51661 gccgtttgct cccgctcacc aagggacatc gacgggcgcg gaaatgaccg ctgtaatgtg
    51721 cgactggcgg gaaacgccaa cataatgtga tgacgctgcg gcagttcgcg actccacggt
    51781 aacaacgttt tagccagctg ctgcgcatca acaatccgcc catctttgat aatgtcgtgc
    51841 gccagcggca accgccacca gcgttgcaaa aagcattctt ttgcgccccg cacgatcgca
    51901 accgctgccg cttcttgctg ttgtaaatgc aaaccaattt gccagatctt aaatgtcatt
    51961 gtgatgatct ccttatcacc cgtcactctg acgggtatat caatgcgtct ggcttgcctt
    52021 tatactaccg cgcgtttgtt tataaactgc ccaaatgaaa ctaaatggga aatttccagt
    52081 gaagttcgta aagtattttt tgatccttgc agtctgttgc attctgctgg gagcaggctc
    52141 gatttatggc ctataccgct acatcgagcc acaactgccg gatgtggtga cattaaaaga
    52201 tgttcgcctg caaattccga tgcagattta cagcgccgat ggcgagctga ttgctcaata
    52261 cggtgagaaa cgtcgtattc cggttacgtt ggatcaaatc ccaccggaga tggtgaaagc
    52321 ctttatcgcg acggaagaca gccgcttcta cgagcatcac ggcgttgacc cggtggggat
    52381 cttccgtgcg gcaagtgtgg cgctgttctc cggccacgcg tcacaagggg cgagtaccat
    52441 tacccagcag ctggcgagaa atttcttcct cagtccagaa cgcacgatga tgcgtaagat
    52501 taaggaagtc ttcctcgcga ttcgcattga acagttgctg acgaaagacg aaatcctcga
    52561 gctttatctg aacaagattt accttggtta ccgcgcctat ggtgtcggtg ctgcggcaca
    52621 agtctatttc ggtaaaacgg tcgaccaact gacgctgaac gaaatggcgg tgatagccgg
    52681 gttgccgaaa gcgccttcca cctttaatcc gctctactca atggatcgtg ccgtcgcgcg
    52741 gcgtaacgtc gtactgtcgc ggatgctgga tgaagggtat atcacccagc aacagttcga
    52801 tcagacacgc actgaggcga ttaacgctaa ttatcacgcg ccggagattg ctttctctgc
    52861 gccgtacctg agcgaaatgg tgcgccagga gatgtataac cgttatggcg aaagtgccta
    52921 tgaagacggt tatcgcattt acaccaccat cacccgcaaa gtgcagcagg ccgcgcagca
    52981 ggcggtacgt aataacgtgc tggactacga catgcgccac ggctatcgcg gcccggcaaa
    53041 tgtgctgtgg aaagtgggcg agtcggcgtg ggataacaac aagattaccg atacgctgaa
    53101 ggcgctgccg acctatggtc cgctgctgcc tgccgcagtc accagcgcca atcctcaggg
    53161 agcgacggcg atgctggcgg acgggtcgac cgtcgcattg agtatggaag gcgttcgctg
    53221 ggcgcgtcct taccgttcgg atactcagca gggaccgacg ccgcgtaaag tgaccgatgt
    53281 tctgcaaacg ggtcagcaaa tctgggttcg tcaggttggc gatgcatggt ggctggcaca
    53341 agtgccggaa gtgaactcgg cgctggtgtc gatcaatccg caaaatggtg ccgttatggc
    53401 gctggtcggt ggctttgatt tcaatcagag caagtttaac cgcgccaccc aggcactgcg
    53461 tcaggtgggt tccaacatca aaccgttcct ctacaccgcg gcgatggata aaggtctgac
    53521 gctggcaagt atgttgaacg atgtgccaat ttctcgctgg gatgcaggtg ccggttctga
    53581 ctggcagccg aagaactcac caccgcagta tgctggtcca attcgcttac gtcaggggct
    53641 gggtcagtcg aaaaacgtgg tgatggtacg tgcaatgcgg gcgatgggcg tcgactacgc
    53701 tgcagaatat ctgcaacgct tcggcttccc ggcgcaaaac attgtccaca ccgaatcgct
    53761 ggcgctgggt tcagcgtcct tcaccccaat gcaggtggcg cgcggctacg cggtcatggc
    53821 gaacggcggc ttcctagtgg acccgtggtt tatcagcaaa attgaaaacg atcagggcgg
    53881 cgtgattttc gaagcgaaac cgaaagtagc ctgcccggaa tgcgatattc cagtgattta
    53941 cggtgatacg cagaaatcga acgtgctgga aaataacgat gttgaagatg tcgctatctc
    54001 ccgcgagcag cagaatgttt ctgtaccaat gccgcagctg gagcaggcaa atcaggcgtt
    54061 agtggcgaag actggcgcgc aggagtacgc accgcacgtc atcaacactc cgctggcatt
    54121 cctgattaag agtgctttga acaccaatat ctttggtgaa ccaggctggc agggtaccgg
    54181 ctggcgtgca ggtcgtgatt tgcagcgtcg cgatatcggc gggaaaaccg gtaccactaa
    54241 cagttcgaaa gatgcgtggt tctcgggtta cggtccgggc gttgtgacct cggtctggat
    54301 tggctttgat gatcaccgtc gtaatctcgg tcatacaacg gcttccggag cgattaaaga
    54361 tcagatctca ggttacgaag gcggtgccaa gagtgcccag cctgcatggg acgcttatat
    54421 gaaagccgtt cttgaaggtg tgccggagca gccgctgacg ccgcctccgg gtattgtgac
    54481 ggtgaatatc gatcgcagca ccgggcagtt agctaatggt ggcaacagcc gcgaagagta
    54541 tttcatcgaa ggtacgcagc cgacacaaca ggcggtgcac gaggtgggaa cgaccattat
    54601 cgataatggt gaggcacagg aattgttctg attaaaaagg cgcttcggcg ccttttcagt
    54661 tggctgacaa agtgcatttg tttatgccgg atgcgggtga acgcgttatc cggctaacaa
    54721 aatcgtgaaa aatcaatata ttgcaggaac cccataagcc tgataagcgt tgtgcatcag
    54781 gcaaacttca cgcatttaca ctcgcccctg ccctttcaac cattcgcgca cgaggaacag
    54841 cgcgctgaca ttacgcgctt cgttgaagtc agggtcttcc agcaaatcca tcatatgcgc
    54901 cagcggccag cgcacctgtg gtagcggctc tggctcatcg ccttccagcg attccgggta
    54961 gagatcttgc gcgaccacga tattcatttt gctggaaaag taagacggtg ccatgctgag
    55021 cttcttcaaa aaagtcagat cgttcgcacc aaatccaacc tcttctttta gctcgcggtt
    55081 agcggcttcg tagacgcttt cacccggatc aattaatcct ttcgaaaaac ctaattcgta
    55141 ggattcagtt cccactgcgt attcgcggat caggatcagg tgatcgtcca caatcggcac
    55201 aatcatcact gcttcccggt tggttggacg cattcgttca taaacacgcc gcacgccatt
    55261 gctgaactcc agatccacgc tctcgacggt aaacagtcgg gaacgggcta cagtttcaac
    55321 attcagaatg gtgggttttt gtaatgattt gttcatcgtg ggatctatgc tgtgaaatca
    55381 gcggttattg tgcgatatcg gacacgcttt cggcaatgtg aattgcatgt tatttacatt
    55441 tatgtaactt aataaataat cgtcctcaaa tcaaattaaa agtcaatagg ttgaaataac
    55501 tccaggaatt tgctgatatt ccgccttcag agggtttgct atgatcagcg gttactgtga
    55561 tgtgcttaat gatgctcaag ttaagctcca cgcttgccga tagccaaccg cagaatcatg
    55621 tattgtgtcc ggtgcgactg accacgcctg acagactaag taagatgggg aaagcatgag
    55681 caccattgtg atttttttag ctgctttgct ggcctgctca ctacttgcgg gatggctgat
    55741 aaaagtgcga tccagacggc gtcagctgcc ctggaccaac gccttcgcgg atgcgcaaac
    55801 gcgtaaactc acacctgaag aacgtagcgc cgttgaaaat tatcttgaga gcctgacgca
    55861 ggtattacag gtgcctggcc caacggaagc tagcgcggca ccgatctctc tggcgctgaa
    55921 tgccgaaagc aacaacgtca tgatgctgac acacgctatc acacgttacg gcatctctac
    55981 cgacgatccg aataaatggc gttactacct cgattcggta gaagtccacc tgcccccctt
    56041 ctgggaacag tacatcaacg atgagaatac cgttgaactg attcataccg attcgctgcc
    56101 gttggttatt tcactcaacg gtcatacgct gcaggagtac atgcaggaaa cgcgcggcta
    56161 tgccttgcaa cctgttccgt ccacgcaggc gtcgattcgc ggggaagaga gtgagcaaat
    56221 cgagctactc aatattcgca aagaaacgca tgaagaatat gcgctgagtc gtccgcgcgg
    56281 gctgcgtgaa gcgttgctga tcgtcgcctc cttcctgatg ttctttttct gcctgattac
    56341 cccggatgta tttgttccgt ggctggcagg cggcgcatta ctgctgctgg gcgcaggtct
    56401 gtgggggcta ttcgcgcccc cggcaaaatc ctccctgcga gaaattcatt gtctgcgcgg
    56461 tacaccccgt cgttggggat tgtttggcga aaacgatcag gaacagatca acaatatttc
    56521 actcggtatt atcgacctgg tctatcccgc acactggcag ccatacattg ctcaggatct
    56581 cggtcaacaa accgatattg atatctatct cgaccgccac gtagtgcgtc aggggcgata
    56641 tctttcgctg catgatgaag taaaaaactt tccgttacag cactggctgc gcagtacgat
    56701 tatcgctgcg ggctcgctgc tagtgctgtt tatgctgtta ttctggatcc cgctggatat
    56761 gccgctgaaa ttcactctct catggatgaa aggcgcgcag accattgaag ccaccagcgt
    56821 aaaacaactg gctgacgctg gcgtgcgggt aggcgataca ttgcgtatta gcggtacggg
    56881 aatgtgtaat attcgcactt ccggaacctg gagcgcgaaa accaattcac cttttttacc
    56941 gtttgactgc tcgcagatca tctggaatga cgcccgctca ttaccattac cagaatctga
    57001 actggtcaac aaagcgacgg cattgactga agcagttaat cgccagctgc accctaaacc
    57061 ggaagatgaa tctcgcgtca gtgcctcatt acgttcagcg attcaaaaat ccggcatggt
    57121 attgcttgat gattttggcg acattgtact gaagacagcg gatttatgtt ctgccaaaga
    57181 tgactgtgtg cgactgaaaa atgcgctggt caatctcggc aacagtaaag actgggacgc
    57241 gctggtaaaa cgcgccaacg ccgggaagct cgatggcgtg aatgtgttat tacgcccggt
    57301 gagtgctgaa tcgctggata acctggtggc aacctccacc gcgccgttta tcacgcatga
    57361 aacggcgcga gcggcacaat cactaaacag tccggccccc ggcggattcc tgattgtcag
    57421 cgacgaaggc agcgattttg ttgatcagcc ctggccttcg gcatcacttt acgactaccc
    57481 gccgcaagaa cagtggaacg ctttccagaa actggcacaa atgctgatgc atacgccgtt
    57541 taacgccgaa ggtatcgtca caaaaatctt cactgacgcc aatggtacgc agcatattgg
    57601 ccttcatccg atcccggatc gttccggcct gtggcgctat ctcagcacca cattgctgct
    57661 actgacgatg ctgggtagcg ccatttacaa tggcgtacag gcctggcgtc gttaccagcg
    57721 tcatcgcact cgcatgatgg agattcaggc ctattatgaa agctgcctga acccgcaact
    57781 gatcacccct tcagaaagcc ttatcgaata acacgtttgc gcggcaggtt atgctaccct
    57841 gtcgcgcaaa ttgcttcact ctggagattt ccctcatgca tatcaacatt gcctggcagg
    57901 acgtagatac cgttctgctg gatatggacg gcacgttgct cgacctcgcc ttcgataact
    57961 atttctggca aaagctggtg cctgaaacat ggggcgcgaa aaacggggtt acgccacagg
    58021 aagcgatgga atatatgcgc cagcaatatc acgacgtaca gcatacgcta aactggtact
    58081 gtcttgatta ctggagtgag caactgggtc tggatatctg tgcgatgacc accgagatgg
    58141 gaccgcgcgc cgtactgcgt gaagatacca ttccgtttct tgaggcactg aaagccagcg
    58201 gtaagcagcg aattttgctc accaatgctc atccgcacaa cctggcggta aaacttgagc
    58261 atacgggtct ggacgcacac cttgatttat tactttccac ccacacattt ggttatccga
    58321 aagaggatca gcggttatgg catgcggtgg ccgaagctac gggtctgaaa gctgaaagaa
    58381 cgctgtttat tgatgacagc gaagcgattc tcgatgctgc cgcgcaattt ggtattcgtt
    58441 actgcctcgg cgtgactaat cctgattccg ggattgccga gaaacagtat caacgccatc
    58501 cgtcactgaa tgactaccgc cgcctgatcc cctcgctaat gtgaaggaga cgccatgaaa
    58561 gagaaacctg ctgttgaggt tcgactggat aaatggctat gggctgcccg tttttataaa
    58621 acccgcgcgc tggcccgtga aatgattgaa ggcggtaagg tgcattacaa cgggcagcgc
    58681 agcaagccga gcaaaatcgt cgagctgaat gccacgctca ctctgcgcca gggaaatgac
    58741 gaacgcacgg tgattgtaaa ggcgattact gaacagcgtc gccccgccag cgaggcagcc
    58801 ttgctgtatg aagagactgc ggaaagtgta gagaaacgcg aaaaaatggc gatggcacgt
    58861 aaacttaatg ccttaaccat gccgcacccg gaccgacgcc cggacaaaaa agagcgccgc
    58921 gacctgttac gatttaaaca cggcgacagt gaataactgt cacctgcaag agagatgatt
    58981 atgccgcaac atgaccaatt acatcgctat ctgtttgaaa attttgccgt tcgcggcgaa
    59041 ctggtaaccg tttcggaaac cctgcaacag atccttgaga accacgatta tccgcagccc
    59101 gttaaaaacg tgctggcaga actgctggtt gcgaccagcc tgttaaccgc tacgctgaag
    59161 tttgatggtg atatcaccgt acagctgcag ggcgacggtc cgatgaatct ggcggttatt
    59221 aacggtaaca ataaccagca aatgcgcggt gtggcgcgcg tgcagggcga aattccagaa
    59281 aatgccgacc tgaaaacgct ggtcggcaat ggttacgtgg tgatcaccat taccccgagc
    59341 gaaggcgaac gctatcaggg cgtagttggt ctggaaggtg ataccctggc ggcctgccta
    59401 gaagattact ttatgcgttc tgaacagctg ccgacgcgcc tgtttattcg caccggcgac
    59461 gtagacggca aaccggctgc aggcggtatg ttgttgcagg taatgcctgc gcaaaatacc
    59521 caacaggacg actttgacca cctggcgacg ctaaccgaaa ccatcaaaac cgaagaactg
    59581 ctgaccttac cggcaaacga agtgttgtgg cgtttgtatc acgaagaaga ggtgacggtt
    59641 tacgatccgc aggatgtgga gttcaaatgc acctgctcgc gtgaacgttg cgccgatgcg
    59701 ctgaaaacgc tgcctgatga agaagttgat agcatcctgg cggaagatgg cgaaattgac
    59761 atgcattgtg attactgcgg taaccactat ctgttcaatg cgatggatat tgctgaaatc
    59821 cgcaacaacg cgtctccggc agatccgtaa gttcattaat gagtatgtcc ggcagagagg
    59881 gctctgccgg attttacgac ttaatggcct gatgtgctgc gcttaccagc gcctatgaaa
    59941 taaatcgcaa attattttgg tcagatacag cgtcgtatcc gataaaagcg ttgtcactta
    60001 cttaagttta tttccagcgt ttcaccatct accgtcagat cgcgccacgc tttcggcctc
    60061 gtccccagtt ttccatcctt cgcagctgtg agtaattttt ctttcgttat ttcgttcgga
    60121 agtaaactca tatcaagcgc gtcccattct tttctgtctg agtcaaagcc atagacaatg
    60181 actctgtcat cattaaaagc aaacaggatc cgctccgcct gaccatcact attcagatct
    60241 tgctcgacca gtacgcaggc gtctttttca atacaggtca tcacgttata gcgatcctgg
    60301 attaaggccg accagaatgt cgcatccggt ttaacagaac caggggcaat taacacattt
    60361 tcggcgaata ctttttccga tacctgttcc tgaagatgtt gctctccatc taacgccatc
    60421 agcagatccc gcgcgcgttt cgggtctttc ataaacccgg catcgctttt cagcgactca
    60481 agcgccgcac gtccatagcg accgctctgt tcgagcatgt agatagttac ctggtctggc
    60541 gtgtttttgc cgctctgata acgcgccata tgactgttca cgctaatacg catactgtcc
    60601 agcaccggcg aattaagaag caccagtatc accaacacca ataacgaaac cgcaaggttc
    60661 actttgccct gaagaacaag gggattttgc ccattacgcc agacgatact gacaaaatac
    60721 cccagcgacc acaccagtaa caccagctcc gccagcgcgc cctgcaagcg gtcgacagtc
    60781 cagccgtact gagcgacccg cagccataac ccccaggtgg cgacgaacac atacagcgga
    60841 gcaaccagca aagcggtttt aatcaggcaa cgcaacggcc ccgtccaggg aagtgacgct
    60901 ttttgcggat cgcggacaat agccattaag atcaattgca aaaaggccag cgttaacagc
    60961 aacccggcgg cggagatgtg gcgagaaatc gcgctcaggc ccgtaaacgg cagggtgatg
    61021 ataaacatca gggttagcaa tgatactaac ggcagcaacc ccgtggcgat tagcgtgaac
    61081 aacttttgaa tagagtcgat taaacgtgac tgtgtacgcg cgaggatcac cgccagtgcg
    61141 gtaaccagac caaatgtaag atacataaac cagtcggttg caaaaaagag cgtattgaaa
    61201 aacgtgatgc caacaagttt aaacaactca ccccacagta aaagcaccag ccacgtcagg
    61261 ccattggaga gaaaaatcac cagtaatata agtacgttat gccatactga ctggtaaaag
    61321 tagctatagc ggctactgcc attgcggata cgaaggcttt gttgtatcca cggtagcaac
    61381 aacattgcca tcaacagcag atagcagcca aaatcccaaa tcgccttttc agctctccag
    61441 gggttcatgc catcagtttg ccacttcagc caaccgctca tccctgtcgt ggcaataaac
    61501 accagcgcca gccatcccca aaggcgcttc tgtttaaaag agatcacgga gaagagtaaa
    61561 acggatgaaa acgcgacggt cgcaggcacg ccataaacaa tccaactgtg attttttccg
    61621 gcaagccagg cgataagcag gtagcaaacc agcccctgaa gtaatccggt agcaatcatc
    61681 ccctgaagta atccggtagc aatcatcccc cagcgtgttg ccggtgaaag ttcaacgtta
    61741 tccatgttgg ttatccagaa tcaaaaggtg ggttaattat cgcatccggg cagtagtatt
    61801 ttgctttttt cagaaaataa tcaaaaaaag ttagcgtggt gaatcgatac tttaccggtt
    61861 gaatttgcat caatttcatt caggaatgcg attccactca caatattccc gccatataaa
    61921 ccaagattta accttttgag aacattttcc acacctaaaa tgctatttct gcgataatag
    61981 caaccgtttc gtgacaggaa tcacggagtt ttttgtcaaa tatgaatttc tccagatacg
    62041 taaatctatg agccttgtcg cggttaacac ccccaaaaag actttactat tcaggcaata
    62101 catattggct aaggagcagt gaaatgcgcg ttaacaatgg tttgcccccg caagaactcg
    62161 aggcttatgg tatcagtgac gtacatgata tcgtttacaa cccaagctac gacctgctgt
    62221 atcaggaaga gctcgatccg agcctgacag gttatgagcg cggggtgtta actaatctgg
    62281 gtgccgttgc cgtcgatacc gggatcttca ccggtcgttc accaaaagat aagtatatcg
    62341 tccgtgacga taccactcgc gatactttct ggtgggcaga caaaggcaaa ggtaagaacg
    62401 acaacaaacc tctctctccg gaaacctggc agcatctgaa aggcctggtg accaggcagc
    62461 tttccggcaa acgtctgttc gttgtcgacg ctttctgtgg tgcgaacccg gatactcgtc
    62521 tttccgtccg tttcatcacc gaagtggcct ggcaggcgca ttttgtcaaa aacatgttta
    62581 ttcgcccgag cgatgaagag ctggcaggtt tcaaaccaga ctttatcgtt atgaacggcg
    62641 cgaagtgcac taacccgcag tggaaagaac agggtctgaa ctccgaaaac ttcgtggcgt
    62701 ttaacctgac cgagcgcatg cagctgattg gcggcacctg gtacggcggc gaaatgaaga
    62761 aagggatgtt ctcgatgatg aactacctgc tgccgctgaa aggtatcgct tctatgcact
    62821 gctccgccaa cgttggcgag aaaggcgatg ttgcggtgtt cttcggcctt tccggcaccg
    62881 gtaaaaccac cctttccacc gacccgaaac gccgcctgat tggcgatgac gaacacggct
    62941 gggacgatga cggcgtgttt aatttcgaag gcggctgcta cgcaaaaact atcaagctgt
    63001 cgaaagaagc ggaacctgaa atctacaacg ctatccgtcg tgatgcgctg ctggaaaacg
    63061 tcaccgtgcg tgaagatggc actatcgact ttgatgatgg ttcaaaaacc gagaacaccc
    63121 gcgtttctta tccaatctat cacatcgata acatcgttaa gccggtttcc aaagcgggcc
    63181 acgcgactaa ggttatcttc ctgactgctg atgctttcgg cgtgttgccg ccggtttctc
    63241 gcctgactgc cgatcaaacc cagtatcact tcctctctgg cttcaccgcc aaactggccg
    63301 gtactgagcg tggcatcacc gaaccgacgc caaccttctc cgcttgcttc ggcgcggcgt
    63361 tcctgtcgct gcacccgact cagtacgcag aagtgctggt gaaacgtatg caggcggcgg
    63421 gcgcgcaggc ttatctggtt aacactggct ggaacggcac tggcaaacgt atctcgatta
    63481 aagatacccg cgccattatc gacgccatcc tcaacggttc gctggataat gcagaaacct
    63541 tcactctgcc gatgtttaac ctggcgatcc caaccgaact gccgggcgta gacacgaaga
    63601 ttctcgatcc gcgtaacacc tacgcttctc cggaacagtg gcaggaaaaa gccgaaaccc
    63661 tggcgaaact gtttatcgac aacttcgata aatacaccga cacccctgcg ggtgccgcgc
    63721 tggtagcggc tggtccgaaa ctgtaatgat ttgaagctgg agaatatcta tccagtatct
    63781 tatagaaagc aaaacgggag gcaccttcgc ctcccgttta tttacccttc ttttgtcgtg
    63841 ccctgcgccc gcgttaccgg cactggcagc caggcgcgaa tggaaagccc gccccgctcg
    63901 ctggtgccaa gctccagcat cccgttatgg ttatccacga tacgctgcac aattgccagc
    63961 cctaatcccg tgccgctaat ggtgcgcgca ctgtcgccgc ggacaaacgg ctggaacagg
    64021 tgcttacgtt gttccggcgc aattcccgga ccgtcatctt ccacctggaa ccaggcgcga
    64081 ttcggctccg ttccgctgct gactttgatc cagccattgc cataacgggc ggcgttgacc
    64141 accatattcg ccaccgcgcg tttgatcgac agcgggtgca ttttcacttc aatgctgccg
    64201 gggtaaagcg cggtttcaat ttcccgctca tagccacttt cggcagcaat cacctcaccg
    64261 agcactgcat taagatccgc catttccatc ggcatctcct gcccggtgcg caggtagtcg
    64321 ataaactgct caatgatggc gttgcactct tcgatatctt tattgatcga ttctgccaga
    64381 tagccatcct gctcgctcat catctcagtc gccaggcgaa tacgcgtcag cggcgtgcgc
    64441 aagtcgtgac ttacccccgc catcagcagc gtgcggtcat ccgccagttg cttaacacca
    64501 gccgccatat ggttaaaggc acgggtaacg gaacgcacct ccgaagcgcc atattcacgc
    64561 agcggcggcg gaataatccc tttaccaacc tgcaaggctg cgtgttcgag atcgaccaac
    64621 ggtcggttct ggatacgaat aaacagccac gccccgccta tcgccaatag cataatcgcc
    64681 agcgtatagc ggaacagcgg agagaaatcg ccctgatgaa tttcggtcag cggcacgcgt
    64741 acccagatat tgggcgacag ccaggttttc agccagacga caggcgaact tttgttgacc
    64801 tcaacgcgca cttccgtcgg gccgcccagt tgctgtgcca tctgatggct taagaattca
    64861 tagtgttgcg cccaacgcag acctgcctct tcggcagcct cgttggagta gagagagatc
    64921 cccagctcac ggtagatctc ccgacggaaa gcgggaggca caaccaactg cgtgccgtcc
    64981 tccagttgca gtttgtcggt catcaacata cgcacttcgt acgcgaggac tttattaaac
    65041 tgctggaggc tcggcaaaat cgcgaagttc agcaccacca gataagtcgt caccaggctg
    65101 gcgaacagca aggtgacgat gagcaataac gtacgggcaa atgaacttcg tggcgagaag
    65161 cgcaatcgcc tcatgcttta gagccgtccg gtacaaagac gtagcccagg ccccaaacgg
    65221 tctgaatgta acgcggatgc gctggatctt cttctaccat gcggcgcaga cgcgaaatct
    65281 gcacgtcgat ggagcgttcc attgcggaat attcacgacc acgggcaagg ttcatcagct
    65341 tatcgcggga gagcggctca cgcggatggc tgaccagtgc cttcagtacc gcaaactcac
    65401 cgctggtgag cggcatcggc tcatcttcgc ggaacatttc gcgcgtaccg aggttaagtt
    65461 tgaacttacc gaaagcaatt accgcctctt cctgtgacgg tgcgcctggc agttcgttcg
    65521 cctgacgacg cagcaccgca cgaatacggg ccagcagttc acgcgggtta aacggttttg
    65581 gaatgtagtc gtcagcgcca atctccaggc ctacgatacg gtccacttct tcccctttcg
    65641 ccgtcaccat aatgatcggc atcgggttgc tctgactacg aagacgtcgg caaatcgaca
    65701 agccatcttc accaggtaac attaaatcca gtaccataag atggaaagat tcacgagtca
    65761 gcaggcgatc catctgttct gcattagcga cgcttcgaac ctggaagcct tgttcggtga
    65821 gataacgttc cagcagcgca cgcaggcgca tgtcgtcatc gaccaccaga atcttgtagt
    65881 tctcttgcat tgtttgtact cccaaaggtt cgcaacaatt tgtaagcgtg tattcttaaa
    65941 aaagctcacg ttcgtcacca gctaaatctg gtatgaattt cagcctaaat tgttacaaag
    66001 catattaaac agcagcttaa gtatacaatt tattcggcga aacattattg attctgttga
    66061 tatgatcacg ttatacccaa tgtgcgcatt atcaaacaga caaagggaat caacgagatg
    66121 aaaacgcccc tggttacccg ggaagggtat gaaaaactca aacaagagct taattatctc
    66181 tggcgtgaag aacgcccgga ggtcacaaaa aaggtgacct gggccgcaag tctgggcgac
    66241 cgcagcgaaa atgctgacta tcagtataat aaaaagcgtc tgcgtgaaat cgaccgtcgc
    66301 gtgcgctatc tcactaaatg cctggaaaat ctcaaaatcg tcgattactc ccctcagcag
    66361 gaaggcaaag tcttttttgg cgcgtgggtg gagattgaaa acgacgatgg cgtgactcac
    66421 cgtttccgta ttgtcggcta cgatgaaatt tttggccgta aagattacat ctctatcgat
    66481 tccccgatgg cccgcgcatt gctgaaaaaa gaagtcggcg atctggcggt ggtgaatacc
    66541 cctgccgggg aagcgaactg gtatgttaat gctatcgagt acgtgaaacc gtaaggaaga
    66601 gtcttaacct cctgccgatg gctggcattt ttgccagcca gtccgtataa ctatcccctg
    66661 atttttgatc cgaaaagatg aactcaaacc atgatgaatg attcgttctg ccgcattatt
    66721 gcgggtgaaa ttcaggcgcg cccggaacag gttgacgctg ccgttcgcct gcttgacgaa
    66781 gggaataccg tgccgtttat cgcacgttat cgtaaggaaa tcaccggcgg tctggatgac
    66841 acgcagctgc gtaatctgga aacgcgtctg agctatctgc gcgagctgga agagagacgt
    66901 caggcgatcc tcaaatccat ttccgagcaa ggcaaactca ccgatgatct ggcgaaggcc
    66961 atcaacgcca ccctaagcaa aaccgaactc gaagacctct acctgcccta caaacctaaa
    67021 cgccgcaccc gcgggcaaat cgccattgaa gcagggcttg agccgttggc tgacctgctg
    67081 tggagcgatc cgtcacacac gccagaagtc gccgctgcac aatatgttga tgccgataaa
    67141 ggcgtggcag ataccaaagc cgcgctggat ggcgcgcgct atatcctgat ggaacggttt
    67201 gccgaagatg ccacgctgct ggcgaaagtg cgtgattatc tgtggaagaa cgcgcatttg
    67261 gtttctacgg tggtgagcgg taaagaagag gaaggggcga aattccgcga ctatttcgat
    67321 catcacgaac cgttgtccac ggtgccttct caccgcgcgc tggcgatgtt ccgtgggcgt
    67381 aacgaaggcg tactccagct ttcgctgaat gccgatccgc agttcgatga gccgcccaaa
    67441 gagagctatt gcgagcaaat catcatggat caccttggcc tgcgcctgaa caatgccccg
    67501 gcggatagct ggcgcaaaag cgtagtgagc tggacctggc gcatcaaggt gctgatgcat
    67561 ctggaaaccg aactgatggg taccgtgcgc gaacgcgcgg aagatgaagc aatcaacgtc
    67621 tttgcccgta acctgcacga tctgctgatg gcggcccctg ccggactgcg tgcaacgatg
    67681 ggcctcgatc cgggtctgcg taccggggta aaagtggcgg tggtcgatgc cactggcaaa
    67741 ctggtggcga ccgataccat ttacccgcac accggacaag ccgcaaaagc agcgatgacc
    67801 gttgctgcct tgtgtgaaaa acataacgtt gaactggtgg cgatcggcaa cggtacagct
    67861 tcccgcgaaa ctgaacgttt ctatctcgac gtgcagaagc agttcccgaa agtgaccgca
    67921 cagaaagtga tcgtcagcga agctggcgcg tcggtttact cagcttccga gctggcagcg
    67981 caggagttcc cggatctcga cgtttcgctg cgtggtgcgg tatctatcgc ccgccgtttg
    68041 caggatccgc tggcggagct ggtgaaaatc gatccgaaat ctatcggcgt aggtcagtat
    68101 cagcatgacg tcagccagac gcaactggcc cgcaaactgg atgcagtagt agaagactgc
    68161 gtaaacgccg ttggcgtcga tctcaacacc gcttctgttc cgctattaac ccgcgtggcg
    68221 ggcctgacgc gcatgatggc gcaaaacatc gtggcctggc gcgatgagaa cggccagttc
    68281 cagaaccgtc agcaactgct gaaagtcagc cgtctggggc cgaaagcctt cgagcagtgc
    68341 gcgggcttcc tgcgcattaa ccacggtgat aacccgctgg acgcctctac cgttcatccg
    68401 gaagcctatc cggtggtgga acgcattctg gcagcaacac agcaggcact gaaagatctg
    68461 atgggtaaca gcagcgaact gcgtaacctg aaagcgtctg actttactga tgaaaaattc
    68521 ggtgtgccga cagtaactga catcatcaaa gagctggaaa aaccgggtcg cgatccgcgt
    68581 ccggaattta aaaccgctca gtttgccgat ggcgtcgaga caatgaacga cctgcaaccg
    68641 ggtatgatcc tcgaaggcgc agtgaccaac gtcaccaact ttggcgcgtt tgtcgatatt
    68701 ggcgtgcatc aggacggcct ggttcacatc tcttcattgt cgaacaagtt tgtggaagat
    68761 ccgcataccg tggtgaaagc gggcgacatt gtgaaggtga aagtgctgga agtggatctt
    68821 cagcgtaaac gtatcgccct gactatgcgt ctggacgagc agcctggcga aaccaactct
    68881 cgtcgcggcg gcggtaatga acgcccgcaa aacaaccgcc cggcagccaa accacgcggt
    68941 cgtgaagcgc agcctgccgg taatagcgcg atgatggatg cgctggcggc agcgatgggt
    69001 aaaaaacgtt aaacgcccgg actggcccct aaacctgcgc gataaggcgt tcacgccgca
    69061 tctggcaacg aacgctttgc ctgacataaa agtgccggag aatatctccg gcacttttat
    69121 tccacagcca aactcacaat atactccgtt aatatttatc acttcattaa caactgaaac
    69181 cttaattaaa cattagccag tccgggtaac tcactattcg aattatattt tcgctgcgat
    69241 ataaccttga gccacatcaa cattgagtca gattattatt caaaccaaca ttcgcacaca
    69301 ttttaagtat tgctgataga aaccattctc attatcattg tgttgttgat tatttaatct
    69361 ctcctttgtt ggcaaatcat ctggtctcat gtcgcggtca aacgccccat gaggtagtta
    69421 tccagttaat gagaaacaag taggcaccta tgcaatacac tccagatact gcgtggaaaa
    69481 tcactggctt ttcccgtgaa atcagcccgg catatcgcca aaaactgctt tctctaggca
    69541 tgttacctgg ctcctctttt gatgtggtgc gcgtcgctcc actcggcgac cccattcata
    69601 tcgaaacccg tcgtgtgagc ctggtattac gcaaaaaaga tctggcctta ttagaagtgg
    69661 aagcggtttc ctgttaatac ggtgataaca acaatgaaaa aattaaccat tggcttaatt
    69721 ggtaatccaa attctggcaa gacaacctta tttaaccagc tcactggcgc acgtcagcgt
    69781 gtaggtaact gggctggcgt taccgtcgaa cgtaaagaag ggcaattctc caccaccgat
    69841 catcaggtca cgctggtgga cctgcccggc acctattctc tgaccaccat ctcatcgcag
    69901 acctcgctcg atgagcaaat cgcctgtcac tacattttga gtggcgacgc cgacctgctg
    69961 attaacgtgg tggatgcgtc taaccttgag cgtaacctgt acctgacgct gcaactgctg
    70021 gaactcggca ttccctgcat cgtggcgctg aacatgctcg acattgccga gaagcaaaat
    70081 attcgtattg aaattgatgc tctatcggca cgtcttggct gcccggtgat cccgctggtt
    70141 tcaacccgtg gtcgcggtat tgaagcgctc aagctggcga ttgatcgcta taaagctaac
    70201 gagaatgtgg aactggtgca ttacgcacag ccgctactca acgaagcaga ttcactggca
    70261 aaagtgatgc cttccgacat cccgctgaaa caacgtcgct ggctgggcct gcaaatgctg
    70321 gaaggcgata tctacagccg cgcctacgcc ggtgaagcgt cgcagcatct ggatgccacc
    70381 ctcgcccgtc tgcgtaatga aatggatgat ccggcgctgc acattgccga tgcgcgttac
    70441 cagtgcattg ctgccatctg tgatgtggta agcaacaccc tgacggcaga acccagccgt
    70501 ttcaccactg cggtagataa aatcgtgctc aaccgtttcc tcggtctgcc gattttcctc
    70561 tttgtgatgt acctgatgtt cctgctggct atcaacatcg gcggggcgtt acagccgctg
    70621 tttaacgtcg gctccgtggc gctatttgtg catggtattc aatggattgg ctacacgctc
    70681 cacttcccgg actggctgac tatcttcctc gcccagggcc tgggtggcgg tattaacacc
    70741 gtgctgccac tggtgccgca gatcggcatg atgtacctgt tcctctcctt ccttgaggac
    70801 tccggctata tggcgcgtgc agcgtttgtg atggaccgcc tgatgcaggc gctgggcttg
    70861 ccggggaaat cctttgtgcc gctgatcgtc ggtttcggtt gtaacgtacc gtcggtaatg
    70921 ggcgcacgta cgcttgatgc accgcgtgaa cgtctgatga ccatcatgat ggcaccgttt
    70981 atgtcctgcg gcgcgcgtct ggctatcttc gcagtattcg cggctgcctt cttcgggcag
    71041 aacggtgccc tggcggtttt ctcgctgtat atgctcggta tcgtgatggc ggtgctgact
    71101 ggcctgatgc tcaagtacac catcatgcgc ggtgaagcga cgccgtttgt catggagctg
    71161 ccggtctacc atgtaccaca cgttaaaagc ctgattatcc agacctggca gcgtctgaag
    71221 ggcttcgttc tgcgtgctgg taaagtgatt atcatcgtca gcattttcct gagcgctttc
    71281 aacagcttct cgttgagcgg gaaaatcgtc gataacatca acgactcggc gctggcttcc
    71341 gtcagccggg tgataacccc tgtcttcaag ccgattggcg tacatgaaga taactggcag
    71401 gcaacggttg gcctgtttac gggcgcaatg gcaaaagaag tggtggtggg tacgctcaac
    71461 accctctaca ccgcagaaaa tattcaggac gaagagttca atccggcgga attcaacctc
    71521 ggtgaagagc tgttcagtgc ggtagatgaa acctggcaga gcctgaaaga caccttcagc
    71581 cttagcgtac tgatgaaccc cattgaagct agcaaaggca acggtgaaat gggtaccggg
    71641 gcgatgggcg tgatggatca gaaattcggt agcgcagctg ccgcatacag ctacctgatt
    71701 ttcgtcctgc tgtatgtacc atgtatctcg gtgatgggag ccatcgcccg tgaatcaagc
    71761 cgtggctgga tgggcttctc catcctgtgg gggctgaata tcgcttactc actggcaaca
    71821 ttgttctatc aggtcgccag ctacagtcag cacccaactt acagcctggt gtgcattctg
    71881 gcggtaatcc tgtttaacat cgtagctatc ggtctgctgc gccgcgcgcg tagccgggtg
    71941 gatatcgaac tgctggcaac ccgcaagtcg gtaagcagtt gctgcgcagc cagcaccacc
    72001 ggtgattgcc attaatggct tcacttattc aggtacgtga tttgctggcg ttacggggcc
    72061 gtatggaagc gacccagata agccagacat tgaacactcc gcagccaatg attaacgcca
    72121 tgctgcaaca gctggaaagc atgggcaaag ccgtgcggat tcaggaagaa cctgacggct
    72181 gcctctctgg cagttgtaaa agctgcccgg aaggcaaagc ctgtctgcgc gagtggtggg
    72241 cgctgcgtta accatactcc atcgcctgtt ttgaaaagcc cggtatgcgt ttgcatccgg
    72301 gcttttttgc gtgcggcttt ccataaaaat gcaattcttg cagcgcaacg taagttcctc
    72361 tgaaagcgtc tcgcagggat gaaaactcgc taatgcacag atgtggagtg gcgcgtagag
    72421 tcgcggcatt caaacaacag gtgaaggaac gccatgagta aaaagcagag ttccacccca
    72481 catgatgcgc tgttcaaact ctttttacgc caaccggaga cggcacgcga ttttcttgcg
    72541 tttcatttac cggcacccat tcacgcgctc tgtgatatga aaaccctcaa gctggagtcg
    72601 aggagcttta ttgatgacga tctgcgtgaa agctattccg atgtgctgtg gtcggtgaaa
    72661 acggaacaag gaccaggata catctattgt ctgattgaac atcaaagcac ctcaaacaaa
    72721 ctgatcgcat ttcgcatgat gcgttacgct attgccgcaa tgcaaaatca ccttgatgct
    72781 ggatacaaaa cgttgccgat ggtggtgcca ttgttgtttt accacggtat tgaaagcccc
    72841 tatccctatt cgctgtgttg gctggattgt ttcgccgatc ccaaactggc aaggcagctt
    72901 tatgcctccg catttccgct gattgatgtc accgtcatgc ctgatgatga aatcatgcag
    72961 caccgacgta tggcgctgct ggagttaatt caaaaacata ttcgtcaacg cgatctgatg
    73021 gggctggtag agcaaatggc ctgcttatta agtagtggat acgctaatga cagacaaatc
    73081 aaagggctgt ttaattacat actgcaaact ggcgacgctg tacgttttaa cgattttatc
    73141 gacggcgttg ccgaacgttc accgaaacac aaggagagtt taatgactat tgcggaaaga
    73201 ttgcggcagg agggggaaca atccaaagcc ctgcatatag ccaaaataat gcttgaatcc
    73261 ggagtccctc ttgcagacat catgcgattt accgggctgt cagaagaaga gttggctgcg
    73321 gcg
//
LOCUS       DAJTUI010000005        71908 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTUI010000005 DAJTUI010000000
VERSION     DAJTUI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.005"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(1362..2948)
                     /locus_tag="OO464_000376"
     CDS             complement(1362..2948)
                     /locus_tag="OO464_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR6225650.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(3303..5474)
                     /gene="glcB"
                     /locus_tag="OO464_000377"
     CDS             complement(3303..5474)
                     /gene="glcB"
                     /locus_tag="OO464_000377"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR6225651.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5496..5900)
                     /locus_tag="OO464_000378"
     CDS             complement(5496..5900)
                     /locus_tag="OO464_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR6225652.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRATKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5905..7124)
                     /gene="glcF"
                     /locus_tag="OO464_000379"
                     /pseudo
     CDS             complement(5905..7124)
                     /gene="glcF"
                     /locus_tag="OO464_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(7135..8187)
                     /gene="glcE"
                     /locus_tag="OO464_000380"
     CDS             complement(7135..8187)
                     /gene="glcE"
                     /locus_tag="OO464_000380"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR6225653.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8187..9686)
                     /gene="glcD"
                     /locus_tag="OO464_000381"
                     /pseudo
     CDS             complement(8187..9686)
                     /gene="glcD"
                     /locus_tag="OO464_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9937..10707
                     /gene="glcC"
                     /locus_tag="OO464_000382"
     CDS             9937..10707
                     /gene="glcC"
                     /locus_tag="OO464_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR6225654.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10831..11972)
                     /locus_tag="OO464_000383"
                     /pseudo
     CDS             complement(10831..11972)
                     /locus_tag="OO464_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12334..14064
                     /locus_tag="OO464_000384"
     CDS             12334..14064
                     /locus_tag="OO464_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR6225655.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            14061..14975
                     /locus_tag="OO464_000385"
     CDS             14061..14975
                     /locus_tag="OO464_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR6225656.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            15008..15256
                     /locus_tag="OO464_000386"
     CDS             15008..15256
                     /locus_tag="OO464_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6225657.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            15256..16427
                     /locus_tag="OO464_000387"
                     /pseudo
     CDS             15256..16427
                     /locus_tag="OO464_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(16463..17542)
                     /gene="lptG"
                     /locus_tag="OO464_000388"
     CDS             complement(16463..17542)
                     /gene="lptG"
                     /locus_tag="OO464_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6225658.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17539..18609)
                     /gene="lptF"
                     /locus_tag="OO464_000389"
     CDS             complement(17539..18609)
                     /gene="lptF"
                     /locus_tag="OO464_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6225659.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18640..19200)
                     /locus_tag="OO464_000390"
     CDS             complement(18640..19200)
                     /locus_tag="OO464_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6225660.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19212..20036)
                     /locus_tag="OO464_000391"
     CDS             complement(19212..20036)
                     /locus_tag="OO464_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225661.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20036..21385)
                     /locus_tag="OO464_000392"
     CDS             complement(20036..21385)
                     /locus_tag="OO464_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR6225662.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21431..22189)
                     /locus_tag="OO464_000393"
     CDS             complement(21431..22189)
                     /locus_tag="OO464_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225663.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22221..22934)
                     /locus_tag="OO464_000394"
     CDS             complement(22221..22934)
                     /locus_tag="OO464_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6225664.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23108..23800
                     /locus_tag="OO464_000395"
     CDS             23108..23800
                     /locus_tag="OO464_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6225665.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23849..25348)
                     /gene="pitB"
                     /locus_tag="OO464_000396"
     CDS             complement(23849..25348)
                     /gene="pitB"
                     /locus_tag="OO464_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR6225666.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25640..27499)
                     /gene="gss"
                     /locus_tag="OO464_000397"
     CDS             complement(25640..27499)
                     /gene="gss"
                     /locus_tag="OO464_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6225667.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27704..28570
                     /gene="yghU"
                     /locus_tag="OO464_000398"
     CDS             27704..28570
                     /gene="yghU"
                     /locus_tag="OO464_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6225668.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28693..28941)
                     /gene="hybG"
                     /locus_tag="OO464_000399"
     CDS             complement(28693..28941)
                     /gene="hybG"
                     /locus_tag="OO464_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6225669.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29075..29416)
                     /gene="hypA"
                     /locus_tag="OO464_000400"
     CDS             complement(29075..29416)
                     /gene="hypA"
                     /locus_tag="OO464_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6225670.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29409..29897)
                     /gene="hybE"
                     /locus_tag="OO464_000401"
     CDS             complement(29409..29897)
                     /gene="hybE"
                     /locus_tag="OO464_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6225671.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29890..30384)
                     /locus_tag="OO464_000402"
     CDS             complement(29890..30384)
                     /locus_tag="OO464_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6225672.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30384..32087)
                     /gene="hybC"
                     /locus_tag="OO464_000403"
     CDS             complement(30384..32087)
                     /gene="hybC"
                     /locus_tag="OO464_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6225673.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32084..33262)
                     /gene="hybB"
                     /locus_tag="OO464_000404"
     CDS             complement(32084..33262)
                     /gene="hybB"
                     /locus_tag="OO464_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6225674.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33252..34238)
                     /gene="hybA"
                     /locus_tag="OO464_000405"
     CDS             complement(33252..34238)
                     /gene="hybA"
                     /locus_tag="OO464_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6225675.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34241..35359)
                     /gene="hybO"
                     /locus_tag="OO464_000406"
     CDS             complement(34241..35359)
                     /gene="hybO"
                     /locus_tag="OO464_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6225676.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35548..35835)
                     /locus_tag="OO464_000407"
     CDS             complement(35548..35835)
                     /locus_tag="OO464_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6225677.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35954..36841)
                     /locus_tag="OO464_000408"
     CDS             complement(35954..36841)
                     /locus_tag="OO464_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6225678.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36998..38038
                     /gene="gpr"
                     /locus_tag="OO464_000409"
     CDS             36998..38038
                     /gene="gpr"
                     /locus_tag="OO464_000409"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6225679.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38078..38572)
                     /locus_tag="OO464_000410"
     CDS             complement(38078..38572)
                     /locus_tag="OO464_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6225680.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38763..39647
                     /gene="yghA"
                     /locus_tag="OO464_000411"
     CDS             38763..39647
                     /gene="yghA"
                     /locus_tag="OO464_000411"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6225681.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39919..40344)
                     /gene="exbD"
                     /locus_tag="OO464_000412"
     CDS             complement(39919..40344)
                     /gene="exbD"
                     /locus_tag="OO464_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6225682.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40351..41085)
                     /gene="exbB"
                     /locus_tag="OO464_000413"
     CDS             complement(40351..41085)
                     /gene="exbB"
                     /locus_tag="OO464_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6225683.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41337..42524
                     /gene="metC"
                     /locus_tag="OO464_000414"
     CDS             41337..42524
                     /gene="metC"
                     /locus_tag="OO464_000414"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6225684.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42664..43323
                     /locus_tag="OO464_000415"
     CDS             42664..43323
                     /locus_tag="OO464_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6225685.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43363..44262)
                     /gene="yqhC"
                     /locus_tag="OO464_000416"
     CDS             complement(43363..44262)
                     /gene="yqhC"
                     /locus_tag="OO464_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR6225686.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            44456..45619
                     /gene="yqhD"
                     /locus_tag="OO464_000417"
     CDS             44456..45619
                     /gene="yqhD"
                     /locus_tag="OO464_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR6225687.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45724..46551
                     /gene="dkgA"
                     /locus_tag="OO464_000418"
     CDS             45724..46551
                     /gene="dkgA"
                     /locus_tag="OO464_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR6225688.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46751..47677
                     /locus_tag="OO464_000419"
     CDS             46751..47677
                     /locus_tag="OO464_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6225689.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47728..47985
                     /gene="yqhH"
                     /locus_tag="OO464_000420"
     CDS             47728..47985
                     /gene="yqhH"
                     /locus_tag="OO464_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6225690.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(48028..50247)
                     /locus_tag="OO464_000421"
     CDS             complement(48028..50247)
                     /locus_tag="OO464_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR6225691.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(50358..51770)
                     /gene="ftsP"
                     /locus_tag="OO464_000422"
     CDS             complement(50358..51770)
                     /gene="ftsP"
                     /locus_tag="OO464_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6225692.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51845..52582)
                     /gene="plsC"
                     /locus_tag="OO464_000423"
                     /gene_synonym="parF"
     CDS             complement(51845..52582)
                     /gene="plsC"
                     /locus_tag="OO464_000423"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6225693.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52816..55074)
                     /gene="parC"
                     /locus_tag="OO464_000424"
     CDS             complement(52816..55074)
                     /gene="parC"
                     /locus_tag="OO464_000424"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6225694.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55620..56102)
                     /locus_tag="OO464_000425"
     CDS             complement(55620..56102)
                     /locus_tag="OO464_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR6225695.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(56155..56547)
                     /gene="ygiW"
                     /locus_tag="OO464_000426"
     CDS             complement(56155..56547)
                     /gene="ygiW"
                     /locus_tag="OO464_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6225696.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56699..57358
                     /gene="qseB"
                     /locus_tag="OO464_000427"
     CDS             56699..57358
                     /gene="qseB"
                     /locus_tag="OO464_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6225697.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            57355..58629
                     /gene="qseC"
                     /locus_tag="OO464_000428"
     CDS             57355..58629
                     /gene="qseC"
                     /locus_tag="OO464_000428"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6225698.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58675..59007)
                     /locus_tag="OO464_000429"
     CDS             complement(58675..59007)
                     /locus_tag="OO464_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR6225699.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            59326..59907
                     /gene="mdaB"
                     /locus_tag="OO464_000430"
     CDS             59326..59907
                     /gene="mdaB"
                     /locus_tag="OO464_000430"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6225700.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59938..60252
                     /locus_tag="OO464_000431"
     CDS             59938..60252
                     /locus_tag="OO464_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6225701.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(60300..62192)
                     /gene="parE"
                     /locus_tag="OO464_000432"
     CDS             complement(60300..62192)
                     /gene="parE"
                     /locus_tag="OO464_000432"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6225702.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(62221..62802)
                     /gene="yqiA"
                     /locus_tag="OO464_000433"
     CDS             complement(62221..62802)
                     /gene="yqiA"
                     /locus_tag="OO464_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6225703.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62802..63629)
                     /gene="cpdA"
                     /locus_tag="OO464_000434"
     CDS             complement(62802..63629)
                     /gene="cpdA"
                     /locus_tag="OO464_000434"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6225704.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63654..64076)
                     /locus_tag="OO464_000435"
     CDS             complement(63654..64076)
                     /locus_tag="OO464_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR6225705.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(64077..64706)
                     /gene="nudF"
                     /locus_tag="OO464_000436"
     CDS             complement(64077..64706)
                     /gene="nudF"
                     /locus_tag="OO464_000436"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6225706.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64911..66392
                     /gene="tolC"
                     /locus_tag="OO464_000437"
     CDS             64911..66392
                     /gene="tolC"
                     /locus_tag="OO464_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6225707.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            66540..67211
                     /locus_tag="OO464_000438"
     CDS             66540..67211
                     /locus_tag="OO464_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6225708.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVLMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            67217..68377
                     /locus_tag="OO464_000439"
     CDS             67217..68377
                     /locus_tag="OO464_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6225709.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(68415..69203)
                     /gene="ygiD"
                     /locus_tag="OO464_000440"
     CDS             complement(68415..69203)
                     /gene="ygiD"
                     /locus_tag="OO464_000440"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6225710.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            69346..70119
                     /gene="zupT"
                     /locus_tag="OO464_000441"
     CDS             69346..70119
                     /gene="zupT"
                     /locus_tag="OO464_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6225711.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(70177..70347)
                     /locus_tag="OO464_000442"
     CDS             complement(70177..70347)
                     /locus_tag="OO464_000442"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225712.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70608..71261)
                     /gene="ribB"
                     /locus_tag="OO464_000443"
     CDS             complement(70608..71261)
                     /gene="ribB"
                     /locus_tag="OO464_000443"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6225713.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(71366..71514)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71636..>71908
                     /gene="ubiK"
                     /locus_tag="OO464_000444"
     CDS             71636..>71908
                     /gene="ubiK"
                     /locus_tag="OO464_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR6225714.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPET"
BASE COUNT        17977 a        19128 c        17722 g        17081 t
ORIGIN      
        1 ttacgtaccg tttaaacaaa gacaatacgt ctctttattt ctcataaaac cccaatccct
       61 tacagggctt attgaaaaat ttaaaaatca caaatttctc aatactcgaa atgagatcac
      121 ataagaaatc aaaaagcctt ctcatctatt ttaagttttc ccaacggaaa ataagagctt
      181 tcattaatac tgaaccactt tactgttcta tttgttcagg tattgcatta cggtgtgctg
      241 agatccatcg gtgcatccct gtatcccgcg atgagactaa aatagtcgtg atgtaagctc
      301 gtatcagatt cttagtgaat accacatttg ttataattaa gtgaaaagat acattgctga
      361 tttacattat ttggtttaca aaatgtcatc acttgcagag ttatcgctga cagatacctt
      421 taatctctta ctactttcgt gttattgcaa tacaaccatc cgtaaagcag ggctattttt
      481 agcaggaaat actcaggtgt caacattcga actccaactc tgaaatattc atataagtta
      541 atgattttaa tgattttaat gattttaatg attttaatga ttttaatgat tttaatgatt
      601 ttaatgattt tttcatttat tcacacctaa taaaagtatt caaattcaga tgatttttct
      661 gctcaacaga cacaaacagg caataatact ctccaaagcg aacaatattt ggcattacga
      721 tttaatgctt cataatcacc aaattattta accataaatt ttcttatttc taacaaaatg
      781 attggtgtgt tgttggagtt aaaatacata cataatatta acatctgcca acatttcact
      841 ttccgcaaga cggatgattt cgaatcctca tccacgttac cctatcccaa atatctttat
      901 taataggcag taacgaacac tgttatagca agattaactt attaacctaa cggcatcttt
      961 tatgtaacaa gtggagataa attgcttaaa attgatcctc gacaaatctt aaaagttctc
     1021 attattctgc aaaatcagct ttgcgaataa tcttatgttt ttgggagttt tgggaatttt
     1081 gatctactca ttgttctagt gaaaaatata aataagagaa aaaacgccat tcgtcatgaa
     1141 agcaacagat gaagcaatgt aataacatac taaaattgat ctcatgaaag tgggtacttt
     1201 ataaccagca cagaagccaa gttgtataaa tattaaacaa ttcgcatcgt aaaacggaga
     1261 ctacgtataa agaagccaat cgtaaaaacg gttccctggc gagataaacg acagacaaat
     1321 ggaaagccgg gtgatattta ccttcatccg gctttattga tttacgagac taacatcccg
     1381 gtaaacacat acgcctgcag cagggtgata acgctggcaa aaatcagact gtgcttcacg
     1441 gtgtagcgga acagttcaga ttctcggccc accatgcccg tcgcggcgca ggccacggcg
     1501 atagattgcg gggagatcat cttgccagtt acgccgccgc tggtatttgc tgccaccagc
     1561 agagtgtcag agacgttgat ttgctgcgcc gtggtcgatt gcagtgaacc aaacagggcg
     1621 tttgaagaag tgtccgaccc ggtaaggaat acgcccagcc agccgagaaa cggtgagaaa
     1681 aacgggaaca tcacgcctgt acctgccagt accagcgcca gcgtggtgga catgccggaa
     1741 tagttggtga cgaaggcgaa tgccagcacc atgccaatcg acaatatcgg ccacttcaga
     1801 ctaattagca tttcggcaaa gacgccaatc cctttcttga tgcccacgcc gaggatgaag
     1861 atagagataa tcgccgcaat aaaaatagcg gtgccgccag ccgagagagg gtcgaattta
     1921 aacaccgcat ccattggcgt tggttgggcg acaatgggtg ccgctttcaa cacttgttga
     1981 tgcaaatgag ggatctggaa attaatcacc agtgaataaa acgcgccgcc cggagcaaat
     2041 aacgctttaa acggcttcat ggtccagatg gtgaccagca ccgttaagat taaaaacggt
     2101 gaccacgcac gaatgatttg ccccagacta tattctgaag gcacgggacc gccagaagat
     2161 ggcttattta ccaccatcgc acctgcggat tgccccatgc tgattgccgt ttcggtattt
     2221 ttcggccgcc agactttaag gaataaagcg agtgagacga tactcaccag cgccgaagta
     2281 atatccggca gttccggacc aatatagtta gaggtaaaga actgagtgac agcgaagctt
     2341 cccccagcaa ccagcgccgc tggccacgtc tctttcaccc ctttccagcc gtccatcatt
     2401 gctaccagcc agaacggcac aagaaccgac aggaacggta actgacgtcc cgccattgcg
     2461 ccaatgtgga acggatcgat tcccgttacc tgaccggcga ccagaatcgg cacgcccaac
     2521 gcaccaaacg ccaccgtcgc accaaagcca gcagctcctt ccagcaacgc accaaaggag
     2581 aaaccaatca gtaacacctg caaacgctga tcgtcggtga tggagataac cgagctgcgg
     2641 ataatatcga actgcccgct ggcaacggtt aatttataca ggaacaccgc cgcgacaata
     2701 atccacgcaa ttggccataa tccataaata aagccatagc ccgcagcagc aaatgccata
     2761 tcaatcggca ttttaaaggc gaatatcgca atcaggatag ataatataag ggttattgct
     2821 ccagcaacat gtcctttcag acgtaatacc gcgagtgcaa cgaagaagaa tattatcggg
     2881 atcagggcga ccagagcgga tagccccagt cctcccatcg gcatatacat ttgggtccag
     2941 gtaaccatat tgttcagtct cttattatct ttatatgctt gatatactta aggttgtaat
     3001 aagcaaaaga ggactgaact gtaaaatata ggcgttatac tttacagcaa cagtacgccg
     3061 ctaacgcaat tgctacctct ggcataacaa gtatatcggg taagggtttc tgttccgcac
     3121 acgcaggcgc agagtatcgt taagatgtcc atattgttgt tttaggcccg ctagtaatgc
     3181 gctacgggta tttaatattg ttaaaccctg ataatcgctc cggttatttc cgggataaat
     3241 gtactaccgc agttactatc atagccccga caataaaact tgccggggct tttttgacgc
     3301 tattaatgac tttctttttc gcgtaaacgc caggcgtgta ataacggttc ggtataaccg
     3361 tttggctgtt tcacgccgag gaagattaaa tcgctggcag ctttaaaagc acacgagtta
     3421 gcgaaattcc ccgccatcgg acgataagcc ggatcgccag cattttgctg atcaaccact
     3481 ttcgccatat tctccagcga cgcctgcacc tgttctttgg tcagaatacc gtgacgtaac
     3541 cagttggcga tatgctggct ggagatacgc agcgttgcgc ggtcttccat cagcgccacg
     3601 ttgtgaatat ccggcacttt tgaacaacca atcccctgct ccacccaacg caccacgtac
     3661 cccagaatcc cctgcacgtt gttatccagc tcttgttgga tctcttgcgc cgaccagtta
     3721 gcgttttcag ccaccggaat agtcagcaga tcgtccagca gcggttcaaa ttcagcactg
     3781 aactcggtct gggcaatgtt ggcttgcacg ctctgtacgt tggtatggtg gtagtgcagc
     3841 gcatggagcg tagcagcggt tggtgacgga acccaggctg tgtttgcccc ggcacgcagt
     3901 tggtcgccct tctggctgta catgtctgcc atcaggtccg gcattgccca catgccttta
     3961 ccgatttgcg ctttaccgcg cagcccacag aacagaccgg aaagcacgtt attacgttcg
     4021 taggctttga tccacggcgt cgatttcatc tggtttttac gcagcatcgg gccagcttcc
     4081 atcaccgaat gcatttcatc gccggtacgg tcgaggaaac cggtattgat gaacgccacg
     4141 cggttgcgcg cctgagcgat acagctacgc aagttcagcg aggtccggcg ttcttcatcc
     4201 ataatgccca ttttcagggt attcggtgcc ataccgagca ttgtctcaat gcgggtaaac
     4261 agtttgttgg cgaacgccac ttcctgcgga ccgtgcattt tcggtttcac aatatagacg
     4321 ctgccagtgc gcgagttttt ctgcactttt aaatcataga gggcaatcgc gccagtcatg
     4381 acgccatcaa gaataccttc cgggatttca ttgccttcgc tgtcccaaat cacaggaatg
     4441 gtcatcaaat gacccacgtt gcggataaac agcaacgagc gtccgtgcag agaaatttca
     4501 gagccatcgg cggcggtgta ctgacgatcg tcattcagtt tacgtacgat ttgccgaccg
     4561 tttttctcca ttttctcttg cagagtcccc tgcatcaggc ccagcagatt gcggtacagc
     4621 aggattttat cttccgcatc aaccgccgcg accgaatctt cgcagtcgag aatggtactg
     4681 atagcagctt cgacgataac atcgttgatg tgcgccggat cgtctttgcc aatccgcccg
     4741 ttggcatcaa tttgcagttc aatatgcagg ccgttatttt tcagcaaaat gcaggtcggc
     4801 gcagcggcat cgccacggta accgacaaac tgtgctggag tacgtaacgt ggtttcttta
     4861 ccatttttca actggatgcg taattgttta tcgaccacct taaacgccac cacatcctga
     4921 tagctgccgt tttccagcgg tagtgattca tcgaggaaac gtcgaaccca ggcgataacc
     4981 tgctcaccgc gctgcggatc gtagccactg accatcgccc cttcctgcgg gatgatgtcg
     5041 ctgccgtata acgcatcgta cagtgagccc cagcgagcgt tcgccgcgtt cagcgcgtag
     5101 cgggcgttca ttgccggaac caccagctgt ggccccgcct gactggtgat ttcgctgtca
     5161 atgcccgtgg tttccaccgt cacgcgctcc ggttgcggca ccaggtagcc cagttcacga
     5221 aggaaagatt tataggccgc tttatctttt accggccccg gattgctgcg atgccactca
     5281 tcaagcgccg cctgaatgcg atcgcgttct gccagcaact gacgattttc tggtgccaga
     5341 tcgtgaacga tctcatcaac attgtgccag aatgcagcag cgtccagttc cacgcccggc
     5401 aaaacttctc catccacaaa acgtttaaaa ttggcgtcaa tgcgtaaacg gccctgggtt
     5461 atggtttgac tcattgtttc tctcctcgtt ttcgcttatt tcgccaacac cgctgctgcc
     5521 actttcgcga cctgcgcatc ctgtgctccg gttaaaccag aaacgcccac ggcaccaata
     5581 atttgcccat caacaacaac cggtacgccg ccttccagcg acgtcagtaa cggcgcagtc
     5641 acgaacgcgg tacgtccgtt gttcaccatc tcttcatagc ccttagttgc acgacgcccc
     5701 agcgcggcgg tacgcgcttt ctcctgggag atgtaagccg caatcggcgc gcaatcgtcc
     5761 atgcgactta acgccagcag atgaccgccg tcatcggcaa cagcaatgga aacagaccag
     5821 ttatttttct gcgcctcttc ctgacctgcg gcaataattg cactcgccat ttgctggcta
     5881 agaatgactt tagttttcat ttttttattc cttttcaagg gcttgttcta caatttcaat
     5941 ccagtgacgc acagaggtac gaccggcgct cgccagatgc gtctggcaac caatgttggc
     6001 ggtgacgatc atttccggtt tgccgctttc cagcgcattc attttgttat cccgcagctg
     6061 gcgtgccaga tcgggatgcg ttaacgcata tgttcccgct gaaccgcagc acagatggct
     6121 gtcgggaacg tccgttaagg taaatccaag acgaagtaac actttttcca cttcgccgtt
     6181 cagcttttgc gcatgttgta gggtacacgg gcagtggaag gccagttttt tatcgccgcg
     6241 aattgccagt ttttccagcg gttcctcgcg cagaagttcg actaaatcga ccgccagttc
     6301 actgacctga cgcgctttat cggcatataa cgcatcgttt ttcagcatct gcccatactc
     6361 tttgacgaac gcgccgcaac cgctggcggt ttgcaaaatt gcctcggcac ctgcttcaat
     6421 cgcgggccac caggcatcaa tattattgcg cgcccgtgcc aaccctttct cctgcgcatt
     6481 aagatgatag tccaccgcgc cacaacagcc tgcttcgtta gctgacatga cgctgatccc
     6541 cagacgatcc agcactcgcg cagttgccgc gttggtgttg ggcgaaagcg taggctgggc
     6601 gcagccttcc agcattaaaa cccgacgctt atggcgcagc ggcggacgcg gtttagcttt
     6661 caccgtttca gcaggcagtt ttgctctgac ctgttccggt aaaaacggtc gcagcaccag
     6721 ccctacctgc gtcagcgcac ggaagaccgc cggacgcggc actacctggc gcaatccttc
     6781 gcgcagcatt cgctccggca gtgggcgttt cactttctgc tcgacaatat cacgcccgat
     6841 atccagcaaa ttgtgatagc gcacaccaga aggacaggtg gtttcacaat tgcggcaagt
     6901 gaggcagcga tcgagatgct cctgtgtttt aagcgtgact tcgttgcctt ccagcacctg
     6961 tttaatcaga tagatgcgcc cgcgcggccc gtccagttca tcgcccagaa gctgataggt
     7021 tgggcaggtt gcggtacaaa atccgcagtg aacacaggcg cacaggatgc tgtcggcttc
     7081 cagcgcgcgt tctgccgcat ctcttcggtt aatcgggttt gcatagcctg ctcctcaaag
     7141 ttccgcgtac atgcgaccgg ggttaaacac gccgcaaggg tcgagctgct gtttaagctg
     7201 ctggtgatag cggaataaag gagccgatag cggggcaaag ccaccatctc cggcactaaa
     7261 gcgggtcgca tgaccgccag cgttgcgggc gatgcgatgg atttgattgt catcggctgt
     7321 cgatttcagc cagcgtaacg ccccgcccca gtcgatcagt tgctcgccgg gtaaatccat
     7381 catcggcgca tcgctgggta atgaaatgcg ccataaggta cctggtaacg agaagaacgg
     7441 cagttgttgt tcacgcaatt gctgccagaa ctgacccaca acctcttcgc cacccagcag
     7501 ttcacgcgct gcttttaccg atccttcgcc gccctcaagg cggatccaca acgcattgtc
     7561 gaagtaacat aagccactaa tgggtaatgg ctggagttgc cactcggcga tttcactcat
     7621 ggcttcttgc aggctgattt cccgacgcag gctcagagag gcacgcggtc gcggtaacac
     7681 tttcattgag atttcagtga gcacgccaag acaaccgtag cttccggcca ttaaccgtga
     7741 gagatcgtat ccggcaacgt ttttcatcac ttcgccacca aaacgcagat gttttccagt
     7801 gccggtaatg atgcgcgtgc cgaggacaaa atcgcggacc gaaccgctcc acgggcgacg
     7861 cggccccgcc agcccgcagg cgaccatccc gccccaggtg gcttcttcac cataatgcgg
     7921 cggctcgcag ggaagcattt gcccggcgct ctccagcgcc gcttcaatcg ccaccagcgg
     7981 cgttccggca cgagcggtta tcaccagctc ggtcgggtcg taattaacaa tgccgcgatg
     8041 acaacgcaca tccagcgttt gcccggtgac tgggcgacct aaaaaggctt tgctattgct
     8101 gccctgaatc accagcggcg ttttatcgct aattgcctga ttcacctgct ccagcagcgc
     8161 ctgactgtaa tcacactcgc gtagcatcaa aagcgctcca gttcaggaaa aggtaaatga
     8221 ccgtgatgca catgcatggc accaaattca gcacagcggt gtagcgtggg aatgtttttc
     8281 ccagggttca gcaaaccatc ggggtcaaac gccgccttga ccgcatggaa ggtcgtgatt
     8341 tcatcgctgt tgaactgggc gcacatttga ttgatttttt ctcgcccgat gccatgttcc
     8401 ccactgatgc tgccgccaac ttcaacgcag agttcgagga tcttcccgcc cagctcttcc
     8461 gcgcgggcaa attcaccagg ttcgttggca tcgaaaagga ttaacgggtg catgttgcca
     8521 tctccggcat gaaagacgtt ggcaacacgt aaatcatatt gctgcgataa acgggcaatg
     8581 ccttccagta cgccaggcag ggcgcgacgc gggatggtgc catccatgca gtagtaatcc
     8641 ggggagatac gtcctaccgc cgggaacgca tttttgagac cggcccagaa acgtacgcgc
     8701 tctgcttcgt cctgtgccaa acggacgtca gtcgcgcccg ctttcaacaa gatgtagtta
     8761 acccgctcgc agtcttcctg tacgtcagac tccacgccgt ccagttcgca taacaaaatc
     8821 gcttcggcgt cgacgggata accggcatga ataaaatctt ccgccgcgcg gatcgacagg
     8881 ttatccatca tctccagccc gccggggata atgccattgg cgatgatgtc accaaccgca
     8941 agtccggctt tttctaccga gtcaaagctg gctaacagaa cccgcgccac gggcggcttc
     9001 ggcagcagtt ttaccgtcac ttcggtggtc acgccgagca taccttccga tccggtgaac
     9061 agcgccagca ggtcaaaacc aggtgaatcc agcgcgtccg atcccagcgt cagcgcctcg
     9121 ccgtccagcg tttgcacttc aattttcagc aggttatgta cggtcagacc atatttcagg
     9181 cagtggacgc caccggcatt ttccgccaca ttgccgccaa ttgaacaggc gatttgtgag
     9241 gaaggatccg gcgcgtaata gagattatgc ggcgcaacgg cctaggagat cgccaggtta
     9301 catacgcccg gctgcacgcg cgcgcggcga ccaacggggt taatgtcgag gctctcttta
     9361 aagcgcgcca tcaccaacaa cacacctttt tccagcggca gcgcgccacc agaaagcccg
     9421 gtgcctgcac cacgggtcac caccggtaca cgcaggcgat ggcagacagc cagaatcgct
     9481 gtcacctgtt ccatttgctt aggcagaaca accagtaatg gacgcgtgcg atacgcgctc
     9541 aacccgtcac actcgtaagg aatgatctcc tcatcggtat gcaggatctc aagtccaggg
     9601 acatgctcac gcagtgccat cagtaccgat gtgcggtcga catcgggtaa agcgccatca
     9661 agacgctctt cgtacaagat gctcatgagt aggcttcgct ttgttgtgtt gtgtggcagc
     9721 tgatttttgc gcgctgcttc tgtgaacagt tattaagcgg gcttttcgtt ttcgtctatc
     9781 tctttagcta ccggtcagac catttttttt ccagctctgt gaccttgtct tggttaactt
     9841 aatgttaaat tgatgtaaca taatcacttg cgtgatgtgc gtgttttgcg agttaagaac
     9901 agaaaaattg gtcctacctg tgcacgaggt ccgggaatga aagatgaacg tcgccctatt
     9961 tgcgaagtgg ttgcagagag tatcgaacgg ttaattatcg acggcgtact gaaggtcggt
    10021 cagccgcttc cctcggaacg tcgactgtgt gaaaagctcg gcttctcacg ctccgcactg
    10081 cgtgaagggc tgaccgtgct gcgcgggcgc gggattattg aaacggcgca gggtcgcgat
    10141 tctcatgtcg cacggcttaa tcgggagcag gacaccagcc cgctgatcca tctgttcagt
    10201 acgcagccgc gaacgctgta cgatctgctc gacgttcgcg cattactgga gggcgaatcg
    10261 gcaaggctgg cggcaacgct gggaacgcag gctgattttg ttgtgataac ccgctgttat
    10321 gaaaaaatgc tcgccgccag tgagaaccac aaagagattt cgctgatcga acatgcgcag
    10381 ttggatcacg ctttccatct cgccatttgt caggcttctc acaatcaggt gctggtgttt
    10441 acgctgcaat cattgaccga tctgatgttt aattcagtgt ttgccagcgt aaataatctc
    10501 taccatcgac cacagcaaaa aaagcagatc gatcgccagc atgcgcggat ctacaacgcg
    10561 gtgttgcagc ggctgccgca cgtcgctcag cgcgcagcgc gcgatcatgt acggaccgtg
    10621 aaaaagaatc tccacgatat cgagctggaa ggccaccatt tgattcgctc ggcggtgccg
    10681 ctggagatga acaaggtggg aatgtgagca gagttttacc ctatccccaa ggcgagggga
    10741 ccgcccctgc acttaacagg atctggtgtt aaatctgttc atctataccc tctcccgaat
    10801 gaccgcccct gcgctaaaca ggatctggta ttaaatttgc ttttcataca atcggtaacg
    10861 cttgtacggc tccgccccaa tgcgttccag catgttattc atgcctgtat tggtttcgag
    10921 gatccatgac atctccagcg catcgatctt ccggcgggca aacggatcgc gtaaggcttc
    10981 aatcaataac agcgcaatca ccgggccgat gcggctgaac tgatactcgt cgcgcacgcc
    11041 catcagcggt actcgcgcag ttcgcacacc gctgactttc aaacgccaca gcaattttgc
    11101 ccagccgaag ggaaagagcg atccgttcag atcggcaatc gcctcgttga tgttaggcaa
    11161 gccacaataa acgcgcaggg tgcagaatca atctcagcga tgtagatcat atcgtccggc
    11221 accagatatt taagttggtc gcccatggtc gcgaattcat gttcggtaaa cggcacaaat
    11281 ccccagttgt gctgccagcc agagttgaaa atctcacgca ggatctgcat ctcttcggca
    11341 aaccgctgac gattgatgca gcgaatggtc acctttttgc gcacctgatc catcagtttt
    11401 tttagcgccg gagagaaagt gagatcggtt cgctgcatcc accacgccag taaatcaatg
    11461 cctttgtgat aacccagttg ttcaatatgc gcggcatacc acggtttgcc gtgtggcatc
    11521 atcgcacagg gtggtgtgtc aaaaccttca atcagtaatc cgctttcctg attgatattc
    11581 aggctgaaag gcccgctgat cttacttgca ccttgtgact tcaaccacgc ttccgctgcg
    11641 ccaaacaacg cggcaaaatc ctgcggatca tcaatggcgt caatcatgcc gaaatgaccg
    11701 gtatcttcac cgtaacgctc acggtgcaag gtatctattt gcgcggtaat acgcccaact
    11761 atctgccccg ctttttttgc cacccacgcc tgccagatga tatggtccgt ccccggattt
    11821 ttcgcagaca aatgctcgtt gcgttcaatg aataaagggg gtatccagtt tggatcgtcg
    11881 ggataaagtg atgacggaaa agcgataaat gccttaaggt catttttatt aattactttt
    11941 tcaattttaa tcatcagcag atcgcattcc atatctctgt ggataacaaa taattaatgg
    12001 tgaaaaacag gttgcaacag caacgagcgg catcttaata atcaactcaa tgaatacaaa
    12061 cgacgaataa atagtattaa ttaatgatta tgataaggtc agaggctaca cagaaacgcc
    12121 tcaatgcctt ttgtcaattt aaggcgaccg gacaaggctt cttcttaata tattcataaa
    12181 ttaaatatca ttcataataa aaacaaaaaa cttaaactca ttatttaaat caatgttcat
    12241 tttcacaccg atgaaaatac tatctcattt atcttttatg cctatataat ccaccgcatt
    12301 attgtcattt tatttttgat atccaatttt ttcgtggtgt atatgtctaa taaaatcttt
    12361 acgcattccc tacctatgcg ctatgccgat tttccaacgc tggttgatgc tttggactac
    12421 gccgctctga gtagagccgg aatgaatttt tatgacagac gttgccaact tgaagatcaa
    12481 ctggaatatc agacattaaa aacgcgtgcc gaagctggtg cgaagcggtt gttatcgctg
    12541 aacctgaaaa aaggcgaacg cgtggcgctg attgccgaaa cgagtagcgg gtttgtagag
    12601 gctttttttg cctgtcagta tgctggctta gtcgccgtcc cgttggcgat tccaatgggc
    12661 gtcggtcagc gggattcctg gagcgccaaa ctccagggtt tactggcaag ttgccagccc
    12721 gcagccatta tcactggtga tgagtggttg ccactggtca atgccgcgac gcataacaac
    12781 cccgaattac atgttttaag ccacgcttgg tttaaggcat taccggaagc cgatgttgcg
    12841 ctccagcgtc cagttccgaa cgatatcgcc tacctccagt acacctccgg cagcacccgt
    12901 tttccccgtg gcgtcattat cacccatcgc gaagtgatgg ctaatctacg tgctataagc
    12961 cacgatggca ttaaattacg ccctagcgac cgctgcgtct cctggcagcc tttctaccat
    13021 gatatgggac tggtcggctt tctcctgccc cccgtcgcca cgcaactttc agtagattat
    13081 ttgcgcactc aggattttgc catgcgtcct ttgcaatggc ttaaattgat cagtaaaaat
    13141 cgcggcaccg tttccgttgc gccgccgttt ggctatgaat tgtgccaacg ccgcgtgaat
    13201 gaaaaagatc tcgctgaact ggatctttcc tgctggcgcg tcgctggtat tggtgctgag
    13261 ccgatctccg cagaacaact ccatcaattc gctgaatgtt tccgtcaggt taactttgac
    13321 aataaaactt tcatgccgtg ctacggactg gcagaaaatg cgctggctgt cagcttctct
    13381 gatgaagcct ccggggttgt ggttaacgaa gtggatcgcg acatcctcga atatcagggt
    13441 aaagccgtcg cgccgggtgc agagacacgc gccgtatcga ctttcgtcaa ctgcggcaaa
    13501 gcgttgccgg aacatggtat tgaaatccgc aatgaagcag gtatgccggt cgcggaacgt
    13561 gtggtaggcc atatttgcat ctccggtccc agtctgatga gcggttactt tggcgaccag
    13621 gtttcgcaag acgagattgc cgcgacgggc tggttagaca ccggcgacct cggttatctg
    13681 ctggacggtt atctgtatgt caccggacgc attaaagatc tgattattat tcgtggccgt
    13741 aatatctggc cgcaggatat tgaatatata gcggaacagg aaccggaaat tcattctggc
    13801 gatgcgattg cttttgttac cgcccaggaa aaaatcattt tgcagatcca gtgtcggatc
    13861 agcgacgaag aacgtcgcgg gcagcttatc cacgcgctgg cggcacggat ccaaagcgaa
    13921 tttggcgtga ccgcggctat cgatctgttg ccgccccaca gtattccccg aacgtcctcc
    13981 ggcaagcctg cccgtgcgga agcgaaaaaa cgttatcaga aggcttatgc tgccagtctt
    14041 aatgtgcagg aatccctggc ataaatcaaa ctgtcgcggt gacgggcgct accgggttta
    14101 tcggtaaata tattatcgat aacctgctcg cccgcggctt tcacgttcgc gcattgacgc
    14161 gtactgcccg cgctcacgtc aatgataatc tcacctgggt gcgcggttcg ctggaagata
    14221 ctcattcact tagcgagctg gttgccggag ccagcgtagt ggtccattgc gccgggcaag
    14281 tgcgcgggca caaagaagag attttcaccc gctgtaacgt tgacggcagc ctgcgcctga
    14341 tgcaagcagc aaaagagagc ggcttttgcc aacgttttct gtttatctct tcgctggcgg
    14401 cgcgccatcc cgagctctcc tggtatgcaa aatccaaata cgtcgccgag caacgactgg
    14461 cagcaatggc ggacgaaatt acgctgggcg tttttcgccc gacagccgtg tatggtcccg
    14521 gcgataaaga gttaaaaccg ctgtttgact ggatgctacg cggcctgctg ccacgacttg
    14581 gtgcgccaga tacacaactc tcttttcttc acgtcaccga ttttgcgcaa gcagtgggtc
    14641 agtggttaag cgccgaaact gtacagacgc aaacctatga attatgcgat ggcgtcgccg
    14701 gtggctatga ctggcaacgc gtacgacagc ttgccgccaa agcccgttgt ggttccgtgc
    14761 gaatggttga cattcctctg ccggtactca caggccttgc ggatatcagt accgcgttga
    14821 gtcgcctggc aggtaaagaa cctatgctga cccgctcgaa aattcgtgaa ttaacccacg
    14881 ccgactggtc ggcaagtaat aaccgtattt ctgaagatat caattggttt cccgggatta
    14941 gcctggaaca cgcattacgc aacgggctat tttgattgtc ttgttttaaa ggtgtgttaa
    15001 cgtttctatg gtaaatcgtg aaatagtaat ggattacatc ctttcctgtt tacaggattt
    15061 ggtcgaaaac ggtgtggaaa ttaagcccga cagcgatctc gtcaacgatc ttggcctgga
    15121 gtcgatcaaa gtgatggatc tcttaatgat gctggaagac agattcgata tttccattcc
    15181 tattaatatt ctgctggatg tcaaaacccc tgcgcaatta atggaaactc tacttccatg
    15241 gctggagaat aaataatggg gctatacgat aaatatgcgc gccttgctgg cgagcgtctg
    15301 caatttagtg acaacgggtt aacaccgttt ggcacctgta ttgatgaagt gtattccgcc
    15361 accgaaggcc ggatcggcaa taaaaaagtg attctggccg ggaccaataa ttatctcggt
    15421 ttaaccttta atcatgatgc catcagcgaa ggtcaggccg cgttagcggc ccaaggcacc
    15481 ggcaccaccg gttctcgtat ggcaaacggc agttatgcgc cgcacctggc gttagagaaa
    15541 gagattgccg agtttttcaa tcgccctacc gctatcgttt ttcaaccggt tataccgcta
    15601 atttaggcgt catcagcgca cttgccgacc ataacgccgt ggtgttactg gatgccgaca
    15661 gccacgccag tatttatgac gtctgttctc tcggtggcgc ggaaattatt cgtttccgcc
    15721 ataacgatgc taaagatctg gaacggcgca tggttcgcct cggtgaacgg gcgaaagagg
    15781 cgattatcat cgttgagggc atctacagca tgctcggtga cgttgcgccg ctggcggaaa
    15841 ttgttgatat caaacgtcgt ctcggtggtt atttgattgt cgatgaagcg cattcgtttg
    15901 gcgtgttagg cgcaacgggg cgcggactgg ctgaagctgt cggcgttgaa gatgatgtgg
    15961 atattatcgt tggtactttc agtaaaagtc tggcctcgat tggcggtttt gctgtcgttt
    16021 cggaagcgat ggaagtgctg cgctacggca gccgcccgta tatttttacc gcctctcctt
    16081 ctccgtcctg cattgcgacc gtgcgctcgt cgttaagaac tattgccaca caaccagagc
    16141 tgcggcaaaa gctgatggat aacgccaacc atctgtatga cggtctgcaa aaactgggtt
    16201 atgagttaag ttcacatatt agcccggtag tgccagtaat aatcggttcg aaagaggaag
    16261 gtttacgcat ctggcgcgag cttatctcgc cgggcgtgta tgtgaatctt atcctgccgc
    16321 ctgccgcgcc tgcaggcatc acgctgctgc gctgtagcgt aaatgctgcc cacagtcgtg
    16381 agcaaatcga tgccatcatt caggcatttg ctactctgaa gcagtaatgt gagcccgccg
    16441 tggtgtttgc gccacggcgg gtttaatgtt gtctgtcata ccaatacacc aggctcaacg
    16501 ccaccaacac aaacgctacc ggtaaaccga gcgcggtaac aggtgcgctt aacgcaaata
    16561 gcaatcccag attaaccatg atttgatagc tgatccaggt gagtaagccc acgatgacgc
    16621 ctacagcgag acggctaccc atcccaggcg aacgcggggc actaaaggta aacggcacag
    16681 ccagcaaaat catcgccagg gtcaggatgg gttgccccag tttttcccac agtgcaaggt
    16741 ggtattcgct gctgggttgc ccggtttcct gcaaataatg gatgtaatga ttaagctgac
    16801 ggacagaaaa agtattcccc ggcattgata actcttcaag attcatactg gtgaaggccg
    16861 attgccaggc aagattatct aatgtttcca gcgtttcttt gccattcagc cattttttat
    16921 gattcacacc atgcaggacc cacgttttgt cgtcttcaat ggtggcactg cgtgcataaa
    16981 tgtaggattc aagcgagaga tcgtcgcgat aatgaaatat ctccacgccc accggctggc
    17041 cctgctcgtt cagcgatttc accgtcacaa attcattacc acgcctggcc cacagcatat
    17101 tgccggtaat gtcatcgtcc tcccccaacg cggtggcggt cgatttgatt tgcaacgcct
    17161 gttgctgcaa tggcgacgcc acccactcat ctatcgcgcc taacgaaaca gtccacaata
    17221 tcccggcaac cagcgccacc agtgcaatac ggaagataga aaaccccgtg ctgcgaatgg
    17281 cggtaagctc actgtttttc gataactgcc ccagtccaac aataccaccc aataacgcga
    17341 taaacggcga aagttcgacc agtgtgcgcg gtaaggtcat tagcaccacc agcaccgcct
    17401 gagtccagcg ataaccgccc gggctgacac catccagttc gttaatcagg ttgaaggtgg
    17461 taaaaagcgg cagcaatagc cctgcagcgg cggcaaaacc gagaaagaga tgacggatta
    17521 aatagcgact gaaaacattc atcgggaaaa tttctgcagc aaagagaagt cacgtgcgac
    17581 cagtatcagc aggcccatta gcatgagtcc gggcactaac cacaaaccag gaatgagggg
    17641 aatcgcgccg ttagccacca gtgtacggca gacgtcgccg ccataaaaaa tggcaataaa
    17701 cagcaacgtc agtggcaata acgttgaaaa tcgcccttgc cgcggtttta cccgacttaa
    17761 tgaaatggcc agcagcgcca tcaacaatgc ggtcaggcca cggctttgtc gccattgcag
    17821 ttcggcatga tcggcaggaa aggcggagcg cgccagctcc gtgaccgatt tcgctttgcg
    17881 tttgacgttc gggctttgat ccagcggatt cagatgtaat tgcagattac ggtagatctg
    17941 ttcgttgtcg tcacgcccct gatgatcgag aagataggca gtgccgttgt gcaacataac
    18001 ggtcggtttc tcgggtgatg ggtcaaccac atcaaccgaa ccggcgcgga aaatacgggt
    18061 tcgattggca gtagaagtat aaatcagcgc gtcagtcagg cgattattat cctgatcaac
    18121 cgtctgcgaa aggatcattc gtccgttatc gttagtgtta aatttctttg cccgcaactg
    18181 gcgaacatcc agctccgact gcgactgctg ctccagttga taaatctgcg cataagccca
    18241 cggacgacca tacatcgaca gaagggtgac aaaaatactt agcggtatcg ccagataaag
    18301 aacggctttg tacaagcgtc ccggactgcc ccccgccgca gagatagcgg taatttccga
    18361 gtcggtatac atctgcccta gcgtcacgcc aactgacaca tacagaccaa caggtaacaa
    18421 catctccagt gcaatcagta ctttgtagaa aacgatatcc agcacaacat caagagctaa
    18481 tgtcccattt gccgcttcgg tcagataacg ctgtgcggag tagctggcga aaataaagat
    18541 cagaaaaccg acgataatca gcaccagacg gcgcgtacca cgcatgatgt agtgttcaac
    18601 cagcttcatc tcactaacgc cctcaatcct ttgagttttt tattcattga tcagtttctg
    18661 cgccaacgca atgacatgat ctacctcttc cggcgtcagg ctgccgtcct ttgcccactg
    18721 aatttgcccg gctttattca gcaccagaat ggttgaactc tgctccggca agcgccaggc
    18781 aacgcgtccc tgtccattgc cgtcaataac gaattgcgcc cacggatagc ggcgtttatt
    18841 tttttcgatt ttgccaacaa caaaatagcc agtaccaaag atggcatcat cggtgttcac
    18901 aatggtggtg ggctgaaaac gatcctgcgg aaagtttgcc gcttcaaccg ctttaattaa
    18961 cagtgagttc tttttcttcg ccgacttacg cccggcgata tactgaataa tgcggacttt
    19021 tcccgctaat tgtgtgctgt tccacttttg gtagtgaatt tcgtcgtcgc tattcagaag
    19081 cagctcgccg ccttcctgga tataaacagg ggtaaccgtt ttgccagtga cgaaattgtg
    19141 tgcctgcaca cccggggcta agagcatgat aatcaacgca ataatcaaac gagatgacat
    19201 cattgctccg gctatagcgg aaaacgcgaa atcagcagct ctaagcgctg acaaatatcg
    19261 ctatccagcg ccaacggtgc ataatacgac tggcggtttt ccatatcatc cgccaccatg
    19321 tcgtcgtgtt tatctcccat caggccatta atggcggcga ggaaacggga aatctgtaac
    19381 gcctctttat tttcaggatc aagcgatctt aaggtgtcgt aataagaaac ctgctggctg
    19441 atgatttttc gcacatcatg aaattccctg ccggttagtc gcggtttggc aataatctct
    19501 ttcagggtat gcatttgctt ttgccgatag gcaataaaac tgcttacgct ttctaactgg
    19561 ctatggcgta atgaatagtt caccaacgac cagaccggag agcttaaata aatccgtaac
    19621 aggttgccgt aatacgacac aatatttttc ttaccgttac gaaagccgtc ctgaaaacgc
    19681 cccagaaaaa cggtcgtttt gccgaataaa aatccggcct gatgcttctt taaatacagc
    19741 cgttgcgcaa aacgcaggtg cttgtagcgc gctcgcatgt atttaaattg tttgcgctca
    19801 tcagcagtca gctcatcgcc tttggcttgt ttgcgaacta aacgcagtaa ttcctgacga
    19861 tcaacgccat tgagccagaa ttgcaggcat aacgcgtaat tatcatgaat gatttcggtg
    19921 gtacaactta gtgcaatagt cggcggcaaa ctaacctgtg catcaacgac atcatcttct
    19981 tcaatcgcaa caaatagacg ctcgctcgcc tcaagcggga aatattttct catcatcagg
    20041 atttctctcc ggctgcgtta taaagcaagg aatggcgctg aaaaaatgcc tttaaggttg
    20101 gaattaacga gagcgccacg tccagacatt gcccaccgaa gtaagcaaac gctgctccca
    20161 gcacaccaaa cagatgacag aacatgaaca gcaatacgat gtagccgatt gaggcgatgg
    20221 tttgcgccac gagaaaagcg cgctgttttc ccgccatcaa tagcaatgac tcctgcggga
    20281 agcccagcat cgaaatcaca attgcgccca gcatcacctg aatcaggtca tacgcttcga
    20341 gatacttaac gccaaacacc agcgaaatga gcggcttgcc gacgatcaac accgcgagcg
    20401 ccaccagaat accgattccg cccgccagta aaccggactt cacacctaac aaccacggtc
    20461 tggtggtgcg cggatctaaa cgcatcacct ctgggtaaaa acttttaccc agcaaacccg
    20521 ctggcgttcc ggcggcgtcg aagaatgtca tggcgatttt aaataacccg gcggcagcgg
    20581 gtcctaacac gatccccacc aacactgtgc tgcacgagtt acgcgccgac cagatggagt
    20641 gggcaatgtt ggttgaccag acaaaactcc acgcgccttt aatgtgtcgg gcagactcaa
    20701 acagattcaa tttgaaggcg ttatggatat ttctacggcg taattcgcgc gcggcaaacc
    20761 accagtacat ggtgccgcca accagattcg acacgtacca agcaataaca aaacccacaa
    20821 aaccaaagtc aaaataccag gctgcgacgc tccccgctgc gcgcagaaaa ggtttcgtcg
    20881 cctgctgtac agcaatcaaa tcaaagcgat ctaccgcacg cagaatgccg gtcggcgtgg
    20941 aggaagccat tgaaggaatg agcgtgcaat acagcgccgc cagccaaaag ctttggtcat
    21001 ccagacctaa tgaatgggaa aggaatggca gtaaggcaat gccgccgaca atcgccaccg
    21061 cgccgctgac gatatccagc gagaaggaaa atgagacgac attgcgaaat tgctgcggat
    21121 tattgttggt caaggctggc gttccgtact gaaccaccag ctgccatgtc tgaaacttaa
    21181 taaaatcgct gatcgacttg gcgtacgatt gcacaatcac cagtacacca aacatggcgg
    21241 gcgtcatccc tttaccggca cacgagagcg ccaacagccc cagcaaggcg ctctcgacat
    21301 tactggagcc taaccaggcg ctattgcgaa taatggtgcg aaacgcgcca tctgcaaacc
    21361 aatgtttgat gttaaaaccc gccaattcag cctgacctta tcgttgatag taaaaaatat
    21421 cccgccagcc ttaagttaaa cttcggcggt cagaaacgat ggcaaccaga gaaaccgcct
    21481 tctgtacctg ttccagcact tcgctgtagg gcgctctgga atcaatctca agaatttttg
    21541 tgccgttata gccaatcgtc gacatgacgc cgattttgtc ctgcagctcg gcatactcat
    21601 ggtcaggctt gcgggagatg gcagtatcaa tatcaatccc caaacgaatg attaattcag
    21661 gacggtattg cgccattttt tggtataaac tccgttcgcg ctgcgccaga aacatgctga
    21721 ttttcccggt cgcacgttcg acgccgatcc ccggtccgtc ataataaaag cccgaaattt
    21781 cagcctgcgg gaagcgatcg ctgaccacca gaacgccact ttgcgccagt cgctgaacct
    21841 tgcgtagatt cgccattctt cgcagcgaga agcagtacat aatcaccgcc gcccatagcg
    21901 ccggagattt ggttttcatg ctttgggttt tcgaggattt ggccgccaat cgccgttcca
    21961 gccagacgcc aaccaacggc aatcgtttga ttttgtcgcc gtcttcgccg gagagcagcc
    22021 ccagatagcg gcgctcggtt tgccagtgtt gttgcagcga tttcaccagg tcggtggtca
    22081 gcgtggattt accggtgcca tcacacccga ccaccgcaat cagccccgga atgtagttgg
    22141 gctgcggcgc ggttgtacta ttaacagttt gtgtttgtag tgcatccatt cgttaaatca
    22201 tcccctatcc attttggtga tcaggaaagc gcggcgtgaa ttgcgcgtag tgacgcttgc
    22261 ataatttcat cggctggttg ccgaccatcc agttcaagga tcttggcgcc attgaatgtc
    22321 agttgcggcg taacggcgat tttttcctgt aacgctgcca gttggtggtc aggtttacgc
    22381 gcaaacgcgg tttgttcatc aatgccaaga cgaatcaaca atacgggtaa ataagacgcc
    22441 atccattggt acagcttcaa ctcgcgctgc cttaacattt ttatccaacc gttaccgccc
    22501 gtggtttttg ccaattgcgg gccatcaaag cgaaaacccg gcacttcaac ctgcgggtag
    22561 cggtcggtga tgagcagata gccttgctgg cttttacaca acattttgcg aaacttgtac
    22621 gcccgccagc aggaaagcag atagataacc agcgcagtaa tattgccagg cggagttaag
    22681 ggcttttcgt gtacatgtgc cgctttactt cgcagataac acccacaagg tgcgccaata
    22741 acagggagct gtgaaatcca ttcgccaatt cgcccggacg attgcccgag ataaatgtgt
    22801 tctgttggca ttcttgctgc cagttcattt accaggcttg ccgtgagggt cgatttacct
    22861 gaaccatcac atcctacaat ggcaataaca cgcactggcg tggaattaat tattgacata
    22921 ttacgttgat tcacgaaaaa cccggcagta aataatgtat tgaatattac gtggtccgtg
    22981 ctcagaatat ccgttcaacc ttattgtgac aatgactaat tgtgacattg acctgaatta
    23041 cgctttcata aaaacatatt aaccaaataa atatttttaa tggatattta aattaaagga
    23101 tatattcatg cagtcaataa cacctccatt aattgccgtt attggtagcg atgcttcagg
    23161 caagtcaacg gtgtgtgaac atcttattac cgttgtcgaa aaatatggtg ctgccgaaag
    23221 agttcattta ggaaaacagg ccggaaatgt cggtcgtgca gtgacaaaat tacctttgat
    23281 gggaaaatcc ttacataaaa caattgaacg aaatcaggtg aaaacagcaa aaaaattgcc
    23341 tggaccagtt ccggcgctgg taattacagc gtttgtcgcc cgtcgcttac tgcgctttcg
    23401 tcatatgctt gcctgtcgtc gtcgcgggtt aattgttcta accgaccgtt atcctcagga
    23461 ccaaattcct ggcgcttacg atggtacggt gttcccacct aacgttgaag gtggtcgttt
    23521 tgtctcatgg ctggcaagcc aggaacgtaa agcgtttcac tggatggcga gccataagcc
    23581 tgatctggtc atcaaactca atgttgacct tgaagttgcc tgtgcacgta aacccgacca
    23641 taaacgggaa tcgctggcga ggaagattgc cataacgcca cagttaacct ttggtggtgc
    23701 acaactggtt gatatcgatg ccaatcagcc actggaacag gtgttggttg atgcagaaaa
    23761 agcgattacg gattttatga ccgcgcgtgg ttatcactag tcaaaaatgg aaatgcccga
    23821 tcgccaggac cgggcatttt caggaaggtt aaatcaactg caatgctatc cagtacagcc
    23881 caccagaaag aaaaattgcc gccggtaaag taaacaccca cgccatcagg atgctggtta
    23941 ccgttttacg ctgtaaccca ccgccgtcca ccaccatcgt ccctgctacc gcagacgaga
    24001 ggacgtgtgt tgtggagaca ggcatcccaa tataactggc aaggccgata gacactgccg
    24061 ccgtcatttg tgccgccatg ccttgcgcat acgtcatgcc gcgcttacca atcttctcac
    24121 cgatggtcat cgctacacga cgccagccaa tcatggtgcc aatgccgagc gccagtgcta
    24181 ccgccatgat gatccacacc ggagcgtact caatggtgct taacatatcg ctgcgcagtt
    24241 ttttcagcag gttctggtct tctttactga cgcctggcag tttcgccagc ttcgcggagg
    24301 tatcggagat gcacagcata atgcggcgca gctggctgcg ctgactcacg cttaacggct
    24361 cgtaactttc catattgcct ggcagcatcg ttttaacgcg cgcaatcgca tcaaaggtat
    24421 ttgccggatg acagtgaaac tctgttactt gcgtgccatc agtcgatgct gcaggcaatg
    24481 gaggttccat cgcaatcaac ttctgcggca gttcaggatg ctgttgcagg tagtgttcga
    24541 agttggtaac ggcatcgcgg gtacgggtaa tttcatagcc ggacgcattc atattgacga
    24601 cgaagccagc aggggcaatc cccaccagta ccagcattac caggccgatc cctttctgcc
    24661 cgtcgttcgc gccgtgcgaa aacgccacgc ccgcagcgga aacaatcagc gcaatacgcg
    24721 tccagaatgg cggtttacgt ttgccctttt tctttttgcg atcttccgga atgcggtgaa
    24781 tacggtcacg ctttttcgtc ccactccaga aacggcgcag caggaaaatc agaccacccg
    24841 caatgaccag gccgacgata ggggaaacaa tcagcgagga gaaaattttg gttacttcac
    24901 gcaggtttaa cgcatccatc accgatgagc cggttaacag cgcgttggtt aaaccgatgc
    24961 cgataatcgc accaatcaag gtgtgcgaac tggaggccgg taaaccaaag aaccacgttc
    25021 ccaggttcca gataatcgcc gccagcagca tggaaaagac catcgccaaa ccgtgagttg
    25081 accccatatt cagcaacaaa tcgattggta acatatggac aatggcatag gcaacgctaa
    25141 gtccgcccaa taacacgcca aaaaagttaa aaaatgccgc catcaccaca gcaagttgtg
    25201 gctgcatggc acgagtataa ataacggttg ccaccgcatt cgccgtgtca tgaaaaccat
    25261 tgattgcttc gtagaacaac acaaatgcca gagcaagtaa taacaaaagc cccgtatata
    25321 tatcaaggcc aacaaataaa tttagcatat attagattac gccattttga atttacgaac
    25381 ggacgcatta tcagtgactt taacggcatg ggcaaagtga aatatcattt tttaacgata
    25441 aaaagagtgt tatttcttgt gtatcagaca ataagcattc attcaaaacc tttagaaata
    25501 atcaaaacac aggtattcca ctggttaata tattcacata tgaaatgaat aaatattgga
    25561 atatataaat attgaatatt ttgattaatc cccgtactaa ttattcttca taatcagtac
    25621 ggggttgcta caacatgtat tactttttca ccacaattaa cggttcaata tcactctctt
    25681 ttttgatgac cagtgattca tcaccgcgca aacacgtccc accgtagttg ccgccaacgg
    25741 tgaaggtaca tacctgaatg tatttaccgt ccactttcgg caaacaccac agttgctgat
    25801 agatgttttt ctgctcggca aatttaccgc tggttttgtc cagcacctct tcatgatggc
    25861 tgaccaggtc gatattgcta ccacagcgac cggcgatcgg tttcactgcg taacctgttt
    25921 tcaccaattc atcattaaca gtgaaatcgg tatccagcag gtaacgatgg tgcgggaaca
    25981 gcgaccagag gatcggcaga atcgctttgt tgccggggat caccgtccac agcggctcaa
    26041 agaccagcac ttccgggcgc agcaatacgt cgataagacg cacttcgttt tgcggatgac
    26101 cggtacggat tggcaccgca gcaaactcac ggtcgctaac ttcacgaatc tgatcaaacg
    26161 cggtttccca cgcccaggtt ttccacacgc agttaaccag tcgcccttcc ccatcaatca
    26221 gttgcccggc agcatcccag cccagttcat ccagcccgcg caagatacgc gtttcaaagc
    26281 ccgcctggtg cagcgcctgc tccataaact gcgcgtgata gttttcctcg atatctttgt
    26341 cctgcatgat atggacaaac ggacgtgcac gactgtgttt ccaggcacca gccagttcgt
    26401 taatcagccc ttccgccgga ttgaagccgt tgcctttata gccctgctcc gcccaacgtt
    26461 cgaggatcaa gcccgcttcg gtatgacagg aggcggagtc ggcgttgtat tcgtaaacct
    26521 tcaggccacg ctcatccatg cagaaatcca tacgaccagt gatcatatgg tgacggcgac
    26581 gctgccagga gagacgcaaa cgtggccaga ggattttcgg aatgtcgaac agcgccagca
    26641 ggttgtcatc tttcagcacc ttgtcggttg cgtgaagata catcagatgc agctcgttgg
    26701 tggctttaat tagctcctgc tcggcactct cggtaatggt gtagtaatga tacggatcct
    26761 gattgatcac ctgaccgttg gcctgcacat aggcgttttg tagcggatct ttttcatcca
    26821 gccatttacc gtcaaactgg cctttgtttt ccagacgcgc tccactgatt ttcagcagct
    26881 cgcctgcaat ttccggctgc ggtaagctgt attcagtatc ttccgtctgg atcatccagc
    26941 ccagaatggt ggtgtcatca aaagtgtctt taagggtata gcagccgttt tcgaccacca
    27001 tctccagctc gcgcgtccac tgttgccctt gcggcaacgg ggaatgaatc acgttctgtt
    27061 ccgcaatacg gactttgttg ccatgcaatt gggtaatgat ggcgacatgg ccagtgtctt
    27121 taaattcacc gcctttatcc cagataagaa gcgcacccgc gaccggcgca cgcggcgagc
    27181 cgttaggaaa tgcctgcaat ggcaggatgt tgtcattaac cacttctcgc aggaagcgca
    27241 gcgagaaaat ctcccacgcc atacccacgt cagtaaagac cacaccgtaa ttcagaaaga
    27301 gaaaacggcg agcaaattca acgcattgcc acttgtggcc catatattcg tcgtcgatat
    27361 agctacggaa tacggcgtca tcttcgtatt cctgcggatc gagagaactg taatctgaag
    27421 agtagattgc taccccacct ggggcgtagc ccaataatgt cccgaacggg gcatcctggc
    27481 tggtcgttcc tttgctcatc actttacctt taacaataca acctaagcag ttggcgtaat
    27541 tgttgtgctc tgattacctg ctcagcgaca ttaaccggac agaggtcaga ctaatcatac
    27601 acctcatcgc agcggctggc gcaaagggtt aaaaaattca cattctgtac agcaagtgac
    27661 ctgctacact gcttcaacac taccactcag aaggcaactc actatgacag acaatactta
    27721 tcagcccgcg aaagtctgga cgtgggataa atccgctggc ggcgcgttcg ccaatatcaa
    27781 tcgcccgttt tctggtccga cgcatgaaaa aacgctgccc gttggcaaac atccattgca
    27841 actttattcg ctgggaacgc cgaacggtca gaaagtaacg attatgcttg aggagctact
    27901 ggcgctgggc gttactggtg cagagtacga cgcctggctg attcgtattg gcgatggcga
    27961 tcaattctcc agcggctttg tcgaagtgaa cccaaactcg aagatcccgg cgctgcgcga
    28021 tcatacgcat aatccgccga tccgcgtgtt tgaatctggt tcgatcctgc tttatctggc
    28081 ggagaaattt ggctacttcc tgccgcagga tctggcaaaa cggactgaaa cgttgaactg
    28141 gctgttctgg ttacagggtg ccgcgccgtt cctcggcggt ggttttggtc acttttacca
    28201 ttacgcgccg gtgaaaattg agtacgccat caaccgcttt accatggaag ccaagcgcct
    28261 gctcgacgtg ctggataagc aactggcgca gcataagttt gttgcgggcg atgagtacac
    28321 cattgcggat atggcgattt ggccgtggtt tggcaacgtg gtgctcggcg gcgtgtatga
    28381 tgccgcagag tttctggatg cgggaagtta taaacatgtg caacgctggg cgaaagaagt
    28441 gggcgaacgt ccggcagtga aacgtgggcg tattgttaac cgcaccaacg ggccgctgaa
    28501 cgagcagttg catgagcgcc atgacgccag tgattttgag acaaatacag aagataagcg
    28561 tcaggggtaa gggttggtgt tcgtcgcagc aagccatcca ggccggataa ggcgttcacg
    28621 ccgcatccgg caatcgtgca caatgcctga tgcgacgctg ccgcgtctta tcaggcctac
    28681 aaaagctaat catcacgcgc tggtaatgtc gtaatccatt tggcgcagtg cgtctaatgt
    28741 ggctttggct tcatcttcgt cgatgatgct catggcaaat ccgacgtgta ccagcaccca
    28801 ctggcccagc agatcggcag ggttaccttc gcaaatcagg gcgatattca cgtcgcgctt
    28861 gataccacat acttcaacct gcgcaagctg gtgaatatct tcaccgacag ccagcacctg
    28921 gcctggaacg ccaatacaca ttgttaactc cggttatttc gattgccgga caaacgcgaa
    28981 aaagaacctt tagcgggtgt tccctctcac cctaaccctc tccccggagg ggcgagggga
    29041 ccgtccgtgc gctttttagg cgttgtcctc agtcttattc aacttcaata cttttgacga
    29101 tcagcgaatc gccggtatcg acacgcaacc gctcgccgtg acagagcgga cactgcgcat
    29161 cgtgctgatg aatctccacc acctggctgc aatcccagca ccaggcctgg gcgggtttat
    29221 agacgatatg taaatcgcac ccttgcgcca ccgttccgtg gcagacaatt tcaaaactaa
    29281 aacggacggc gctctcctca acgcaggaga gcgcgccaat ttccagccac acggcggtga
    29341 cgcgcttaac atcgtgctgc tccgcctgcc gttggataat ttcaacggcg ctctggcaaa
    29401 gcgacaactc atgcattttc gcccctcctg cggccaaaca gcagggcgcg acgccctgcg
    29461 tgtggtacat ccggattggt gactggcaac gaaaggatca ttcgtgcgca gtcatccgtc
    29521 aggcgttggc cttcttcaat cgacatgctg tgcgaaagcg gcgacatcag cgaacaggag
    29581 aggtattgcg aaacaccgtc cagttcgcca acggtaaagg tcatggtgcc atacggcagt
    29641 tgcagaccaa ttttttcact cactttgcgc agcggccaga gttgatccgg gccggggaag
    29701 ataactgcac tcagcatcca cggggtgatc acacaccccg tccactgacc ttcgaacagc
    29761 gtaaaatcag aaacatacac cggcattgaa ggatgcagaa aagagagatc gtgcatcgaa
    29821 cgccgggcaa tttcttcaaa cgctgcctgt acttgcgcct tcggggaggt ctggaaacct
    29881 gctatctctt cagtcatgaa tcgcctcccg tgggatggct tccacgcctg attcgcgcag
    29941 cgcagccaga acctgctcaa gcgcaggttc aatcattgct tcaaccgtcg gcgttaagcc
    30001 gatgtgtggc tccagcgatt ccgggatcac gccgaccagg gtcagctttt tcggaaactc
    30061 accggtgaag cgcagggccg acaatacgtc ggccaggcca agctgatgcg gagagatttt
    30121 gttggtaaac aacgccggaa cttcttcatc ccgcaggatc atcatcgttc ccggagtgtt
    30181 ctttttcgac acaatggcat ccgcaataat cagatgatcg cgatttgcca tgtcgccaag
    30241 cagctccatt cccgccgtgc cgccatcgag gatctcaaca taatccggca gaatgtatcg
    30301 ttgctctaac gcttcgacaa tccgcacgcc gatggcttca tcggtcagca aaatattgcc
    30361 gacccctaag actaaaatac gcattacaga accttcactg aaaccacttc gttgccgtcg
    30421 gcatccacta cgtgtaccgc acaggccatg cacgggtcga aggagtgaat ggtacgcacc
    30481 acttccagcg gtttattcgg atcggcaacc ggtgtaccca ccagcgactg ctcgtaagga
    30541 ccgacgtcat cattgaagtt acgcggacca gagttccagg ttgatggaac aaccgcctgg
    30601 tagttgctga tgataccgtc tttaataacc atccagtgag agagcatacc gcgcggcgct
    30661 tcgaggaagc caacaccttt gaactcaccc gttgccggaa tgttcggctt cacaaaggtg
    30721 gtgtgatcgc ctttgccgat attggtgatc agtgcactgt attggttttg caggatatcc
    30781 tgcaattcac agcagtgaac ggtacgacca ataatacggc ccagcgtgga gtgcagctgt
    30841 gccacttcca gcgtgttgcc agtcagtttc tgataaatcg caacgatttc attcagtttg
    30901 ttctgggtag attcgcgacc tgccgccagt ttcaccagca tattagccag cggacccact
    30961 tctaccgttt tgccgtagaa agtcggtgat ttcacccagg aatatttccc gtcgtcagac
    31021 caaccatcat aagccggaat ggtggtacct tcccacggcg cctgtggcgc ttcgtcttta
    31081 taccaggagt gcttcgcgct ttcctgaatg cctttgatca ggtattcatc ggaatgagaa
    31141 gtgatcggac gatacgagga cagatccgca ttctcaatgt agcctcccgg gaacaggaag
    31201 ctgccgtttt tgctgtcggt cgggaattcc ggcacgctca ggtagttcac cgcaccttta
    31261 ccgcgcgtca gccattccgg gtagaacgcg gcgataaccg cggtatcaac cttataaacc
    31321 tgctcaacaa agtcgctcag tttgtcgatg aaagacttga tgtacatcag gcgctcaagg
    31381 ttcaacacgc ccaacccgtc gaggttgatt gggttcgcga caccacccac agccaggttc
    31441 tgaatgtgcg gcgttttacc gcccagcagc gccacgacgc ggttagcgtc acgctggcac
    31501 tccagcgcct gcaggtagtg cgctaccgca atcaggttca cttccggcgg cagtttcatc
    31561 gccgggtgac cccagtagcc attggcgaaa atacccaact gaccgctggc aaccagatct
    31621 ttgatcttgt tctgaacttt agtgaactct tccggactgt tcagatgcca ggtcgaaacg
    31681 cctttcagca tttcggaggc tttggttggg tcagcttgca gtgcagaagt gatgtccacc
    31741 cagtccagcg ccgaaagctg atagaaatga acgatatggt catgcgtggt gtgcgcagcc
    31801 agaatgatgt tacggatgta ttgcgcgtta accggaacgt cgatattcag cgcactttct
    31861 gccgcacgaa cggaagacag cgcgtgagtg gtagtacata cgccacagat acgttgcaca
    31921 atcatccatg catcgcgcgg atcgcggttt ttcacgatct cttccatgcc gcgccacatg
    31981 gtaccggaag cccatgcttt cgaaacgacg ccattttcga tttcgcaatc gatgcgtaaa
    32041 tgcccctcaa tacgggttac cggatcaata gtaattctct ggctcatgct ttgctcgcct
    32101 catgacgatt atgatcgttt tgttttaaag gaggaagtat cggcagtaga cgaatgagta
    32161 cgatgtaagc gcaaatctca atagccacaa aaccaataga aatcaacagt tcttcccagg
    32221 tcgggaagta ggcgtaaccg ccgcccgggt tgaatgccac caacgaatag gtcagacgcc
    32281 aggttgcaca acctaacagt gcgctcagtg ctgacaggaa cagcatacgg gaatcattac
    32341 gcagcttcgc cacacgcaga acgaccagcg ggaagagcat cagcaggact tcaatccaga
    32401 acatcacgga gtagaagtca ccggcaaacg ctaacgacag cttgtcgcga tagataagct
    32461 cgccaaagcg cagcccgatg aaaatcgcca gcaacacact gatggtgttg gtcagcttga
    32521 cgaacaggct cttttcatcc ggaccgttgc cacgcagacc cgcctgcacc agtgaacctt
    32581 caaagatgac aatcgagaag cccatgatga acgccgtcag cagcgagaac agcggcaaca
    32641 tttcatagct ctgccagagc ggatgcacct tgtagcccgc cgagatcatc agcgacccca
    32701 ttgaagactg gtgcatggtc ggcagcagcg caccgagcgc gatgatgaag aacatcacct
    32761 tgttcagacg ttgtagcgac accttccagc ccagacgttc aaacagtgcc ggagcaaact
    32821 ccagagccat cacgccgatg tagatggtca tacagaccgc cgtctcgaac agtaccgagt
    32881 tcacgttgaa gtgacccgga atgtagaagt acggcaggtt ccagtagcga cccacgtcga
    32941 tagtgatcga caagccaccc agtgagtaac caaacagact cgccaacagc gccggacgca
    33001 ccagcggatg gtattgccca cggttaaaga cgtataccgc ccacgccagc gcccagccgc
    33061 cacaggcaaa gccggtgcca atcagcaggt caaacgcgat ccacacgccc cacgggaagc
    33121 cgccgttcag gtcagagaca gagcccagac cgaacaccag acgcttcaca atcaggagca
    33181 tacagatgac gattaacggt ccaaaaatca tgaccggttt actgatgatt ttgccgccca
    33241 gcggttgtgg atcatgactc atgatcgtct cctccgtcgt gatggtcgtt tttggtgttg
    33301 cgacgaacca gcacggttaa gcccgccagc acagccagtg gtagcatcat gcctttatac
    33361 agggtgtgtt gaatattttc ggaacgcgca ccggtagaaa gatcgtccag tttcggcagg
    33421 tcgagatttt cataaggcac acccgtcagt accagaacct gagtaccgcc gccctctttc
    33481 tcgccgtaca gatgcggata atatttcggc accgtatgca ggtaagtgtc gccagatttc
    33541 agcgtctgac gcggatagtg gtattcgctg ccaggcttca gcgccagacg ttttttcgcc
    33601 tccgccatca gctcttcacg cgtaccgaaa atcaccgcgc ccgccgggca cacttctacg
    33661 cagccaggca gaccgccttt atcgagacgt tccacacctt tctggttgca cagctcgcac
    33721 ttatgcagcg caccaaacgg gttgttgtag tcgtacttcg gcacgttgta cggacaggcg
    33781 accatgcagt aacggcaacc ggtgcacacg tctttgtcgt aatggacaat gccggttttc
    33841 ggatcttttt tcagtgcaga gaccgggcac acagagacac agttcggatc gacgcagtgc
    33901 atacactgtt tcttaatgta cgcgtagccg ttctcctcct ggtctttgtt gacccctgtg
    33961 ccgctggtcc acacctgaat gatgttattg gtatacggcg acagtttgtc gttgttcgac
    34021 caggtctgtt ccccttgcgg gttacgttca gggaagttga tatcctgaca cttggtgacg
    34081 caagcctggc agcctacgca caaggtcgag tcgtacaaca tccccagcga tcccggaatt
    34141 ggcgggcggt tttcagcagc cgcatgactg acagacggca gcgcgcccgt cagcaatgcc
    34201 ccgcaggagg ctgctttaat aaaattacgt ctgttcacgg ttattctccc cgtgagtcag
    34261 cgttatcttt cttttgctga cgacccagtt cacgcaccgc catcacgctg acaccggcaa
    34321 ccagcccaac cacaccgccg agcaaaccaa tagcgcctgc agagacgttg ccgccctctt
    34381 tagcgttaac atccggtttc tgtgaacgcg gagtctgatt ttcgacgttg gcaagctgat
    34441 ggatgccttt atggaagccg ataccttttt cgttacagcc atagcacggg tgaccaatcg
    34501 ccaccggcca cacaccgcca acatcgcaga attgcagcgt tgagcagttg ccgtaagttt
    34561 ctggcccttt acagccgagg tggtacaagc accagccttc gcggtggcct tcatcaccga
    34621 actctttggc aaaacgacca gcatcgaagt gcgggcgacg ttcgcagtgt tcgtgaatca
    34681 gacggccata ggcgaaggtc ggacggtttt tgtcatccag tttcggcggt ttgccgtaag
    34741 tgatgatgtg cgcaacggtc gcgaggaagt tgtgcgggtt cggcgggcag cccggaatat
    34801 tgataacggt tttgcctggc agaacttctt gcaagctgac tgcgccagtt gggttaactc
    34861 cagctgcggc aacaccgccc cacgcggagc aggaaccaat agcgataatg gctgctgagc
    34921 cttccgccgc tttgcggatg tgatccacaa tcggctcacc ggcaaccatg caataaatac
    34981 cgttatcttt taatgggatg gaaccatcca ccactaacac atactgccct ttgtacttct
    35041 cgagagcgtt atgtttgttc tcttcgacct gatgaccgaa ggcggcggaa agcacttcgt
    35101 gatattccag agagatagtc tccagcacga ggttttctac cgttggatgc gttgcacgaa
    35161 gcagagattc cgtacaaccg gtgcactcct gcgcgccaat ccagataact ggcggacgct
    35221 gcgggttagt aaccgattcg gccatctctg cagcggcttt gctacttaac cccatggtgg
    35281 cggctaatgc tgcacaaagc ttcatgaaat cacgacggtt aatgccgtga gaatggatga
    35341 gggtgttatc tccagtcatt tatagttatt ccgttgcgag aacctggcat atattttgcc
    35401 tcaatcgcaa aatcaataat gcgatcgatg cgccatttac cacacattta ttatggttat
    35461 cgatatcatg atactgcggc ggggcgctaa aacgaaggga agaagcatta gtgtaattaa
    35521 ttaaagcaag ataatacgta tgtttgatca attttcgagg cgataaccgg ccataaaaaa
    35581 gcgcaacgta gaacaggaat tattctcacg aaagaaatcc attaccatct ctttatccag
    35641 tccatagcgg cgcgtcagaa tacccgcttc ccaggcgcta agctggcgat ctccggtctt
    35701 ttcgtacatc ctgtgtgagt agcctaatac aaaaccgcgt ttataatcgg cacagaattt
    35761 tgtcacatta accgcactgt cggcatgcgt tgcttttaat cccgccatta agcctttacc
    35821 aaaatggtta ttcatcgttc acctcattca ttatatatcg ccatatataa cgatatttta
    35881 ggcaaagaac gacttttgcg cacgctattt tttcatactt gtttaagacg taatcccgta
    35941 agagataaac ctatcaggag agatatttat cgaaccattt cagtgtcctt tgccaggcaa
    36001 gatcggcggc agatttgtca taacggggcg tggaatcatt atggaatccg tgattaaccc
    36061 ccggatagat ataagcctca taaaccttat tattgtcttt caacgccgcc tcgtaaggag
    36121 gccagccctc gttgattcgg gtgtccagtt ccgcgaagtg gagtagtaaa ggcgcttcaa
    36181 tcttcgccac atcggcagtg ggtgcctgac gaccataaaa cggcaccgcg caggccagtt
    36241 ccggatacgc gacagccgcc gcgttcgata cgccaccgcc atagcaaaat ccggtaatac
    36301 ccactttacc tgtcgcttgc ggatagcgtt gcataaactc aattgcggca aagaaatcat
    36361 tcatcagttt ggttggatca acctgctgtt gcagctcacg acctttatca tcatttcccg
    36421 gataacctcc aacggaactt aatccgtcag gtgccagggc gatatacccc gccttcgcca
    36481 ctcgccgtgc cacatcttcg atatacggat tcagtccacg attctcatgc accaccccca
    36541 cggctggcgt tttgccgctc atttttgcgg gtttcaccag ataaccccgt acctcgccgt
    36601 gaccatttgg cgaaggatac gtgatgtact cagcaacaat ctccgggtcg gtaaactcta
    36661 cctgagtcgc cagcgcataa tttggcttga gcaaatcaaa caacgctaat gccgtcatcc
    36721 cgcccaccgc atacttcgcc gcaaggttga ggaactcacg tttcgaaatt ttcccgtgaa
    36781 cgtaatagtc gtagtaatcc aacaactctt gtgggaaatc tttggcggtt aaacgcggca
    36841 tcgttgcatt cctcaactgg tgttttttta agcaaagcat aagcgcgtgt ttttgcccag
    36901 tttttcgtca ctctgtgagc cagactacgg gatacgcgct ggcgaatcgc taaactagaa
    36961 acattgtttc gaaattgaac ggtggaaagg agaggtcatg gtctggttag cgaatcccga
    37021 acgttacggg cagatgcagt accgctattg cggaaaaagt ggtttacgcc tgcccgcatt
    37081 atcgctcggt ttatggcaca atttcggtca cgttaacgcg ctggaatcac agcgtgcaat
    37141 cctgcgtaaa gcgtttgatt taggcattac gcactttgat ttagccaaca attacgggcc
    37201 gcctccagga agcgcagaag agaactttgg tcgcctgctg cgcgaggatt ttgctgctta
    37261 tcgcgatgaa ctgattatct ctaccaaggc tggctacgat atgtggcccg gcccgtacgg
    37321 ctctggcggt tcacgtaaat acctgctcgc cagcctcgac caaagcctga agcgtatggg
    37381 gcttgagtat gtcgatatct tttactctca tcgcgtcgat gaaaatacgc cgatggaaga
    37441 aaccgcctct gcgctggctc atgcggtaca aagcggtaag gcgctgtatg tcgggatctc
    37501 ctcttactcg ccagagcgga cgcaaaaaat ggtcgagttg ctgcacgagt ggaaaattcc
    37561 gcttttaatt catcaacctt cgtacaattt actgaaccgc tgggtggata aaagcggcct
    37621 gctggatacc ctgcaaaata acggcgtggg ctgtattgcc tttactcctc tggctcaggg
    37681 attgctgacc ggaaaatatc tcaacggcat tccgcaagat tcacggatgc atcgtgaagg
    37741 gaataaagtt cgtggtctga cgccgaaaat gcttaccgaa gccaacctca acagcctgcg
    37801 cttattgaat gaaatggcac agcagcgtgg acaatcaatg gcgcaaatgg cgttaagctg
    37861 gttgctgaaa gatgatcgcg tgacgtcggt attgattggt gccagccgcg cggagcaact
    37921 agaggagaac gtgcaggcgc tgaataatct gacatttagc accgaggagc tggcgcagat
    37981 tgatcagcat atcgccgatg gcgagctgaa tctgtggcag gcgtcgtccg ataaatgacc
    38041 tgttaataac gggcgcgcga accgcgcccg aatcagatca gtgattatga cgagtcagtc
    38101 ggtcaagata gcccatcaca aatgcagaaa gcacaaacgt cagatggata atgacgtacc
    38161 acatcagttt gttatccggg acatttttcg catccataaa gacgcgcagt aagtgaatgg
    38221 aagaaattgc cacaatcgac gctgctactt tgtttttcag cgacgttgcg tccattttcc
    38281 ccagccactt cagcttctct ttgttctcgg agatatccag ctgtgagacg aaattctcat
    38341 aaccggaaaa catcaccatc accagcaaac cgccaaccag cgtcatatcc accagcgaca
    38401 gcaacacgag gatcaaatct gattccgcca tcgagaagat attcggcagt acgtgaataa
    38461 tctcctggaa gaacttcagc gccagggcaa ctaacgccag cgaaaggcca aagtacacgg
    38521 gggcaagcag ccagcgagaa gcatacattg cattttcaag aaaacgttcc atacattcct
    38581 gtcgtgtagt taaaacgggc aacagtatat cgcaattgag taaacagcac gcaacagcaa
    38641 atcaacatat acgttaaacc tttgtcgcat aattctgagg tttagggatc ttcctggttc
    38701 ccttatcaac gcatcgagag cgtctaccct taaaggacgt tgaacctgaa gggagaaaaa
    38761 cgatgtctca tttaaaagac ccgaccacgc agtattacac tggtgaatat cccaaacaga
    38821 aacaaccgac gccaggcatc caggcgaaga tgacaccgat accggattgc ggcgagaaaa
    38881 cctatgttgg tagcggtcgc ctgaaagatc gtaaagcact ggtgacaggg ggcgattccg
    38941 gaataggtcg cgctgccgcc atcgcttacg cgcgtgaagg ggctgacgtg gcgatcagtt
    39001 atcttcccgt ggaagaagaa gacgctcagg atgtgaaaaa gatcattgaa gaatgcggac
    39061 gcaaagccgt tctgctgcca ggcgatttaa gcgatgagaa attcgcccgt tcgctggttc
    39121 acgaagcgca caaggcgtta ggcgggctgg atattatggc gctggtcgcc gggaaacagg
    39181 ttgccattcc ggatattgca gacctcacca gcgaacagtt tcaaaagacc tttgccatta
    39241 acgttttcgc gctgttctgg ctaacccagg aagcgatccc cctgctaccg aaaggtgcaa
    39301 gtgtcatcac cacttcgtca atccaggcat accagccaag tccgcattta ctggactatg
    39361 cggctacgaa ggcggcgatt ctgaactaca gccgtggcct ggcaaaacag gtcgcggaga
    39421 aaggtattcg ggtgaatatt gtcgcgccag gccctatctg gacggcactg caaatttccg
    39481 gcggacaaac gcaggataag atcccgcagt ttggtcagaa aacgccgatg aagcgtgcgg
    39541 ggcaaccggc ggaactggcc cctgtatatg tttatctggc aagtcaggag tcgagctacg
    39601 tcaccgcaga agtgcacggc gtgtgcggcg gcgagcattt aggttaaaaa aatgcccggt
    39661 tgtgaaaagc aaccgggcat cattgtgaat tcactcttac ctgatgtggc gaaaacgcct
    39721 tatccgccta caaacgcatg aagattcaac atgttgcaat agattttgta ggcctgataa
    39781 gcgtagcgca tcaggcattg agcacctaat gccggatgcg tcgtgaacgc cttatccggc
    39841 ctacaaacgc acgaagattc aacatgttgc aatagatttt gtaggcctga tgagcgtagc
    39901 gcatcaggca attctacttt acttcgcttt ggcggtttct tcgccgacca gacctatctt
    39961 caggtaaccc gcctgatgca gcgtatccat taccttcatc aacgtctcgt aatcgacggt
    40021 tttgtccgct cggaagaaga tggtggtgtc tttcttgcct tcggttaacg cattcaacgc
    40081 cgtaatcatt gtttcatcgg tgaccggatc gttaccgata aacatcgagt tgtctgcctt
    40141 caccgacaga taaaccggtt tttccgggcg cggctgcggc gtgctggtag aagcaggcaa
    40201 gttcaccttc acatctaccg tcgctaacgg tgccgccacc ataaagataa tcagcagaac
    40261 caacatcacg tcgataaacg gcgtcacgtt gatatcatgc atttcgccgt tatcgtcgag
    40321 gttttcgtta agatgcattg ccatcggaca ttatcctgcg cgtaattttt gtgcgacacg
    40381 aaccggatgc gcagcggcgc tggcttccag atctaggtca cggctttgca gcaacaatac
    40441 ctgcgctgca acatcaccca gcatcgcttt aaagccgcca atctggcgtg caaatacgtt
    40501 atagataacg accgcaggaa tcgctgccac gaggccgatt gccgttgcta acagagcttc
    40561 tgcgataccc ggcgcaacga ctgccaggtt agtggtctgc gtttgcgcga taccaataaa
    40621 gctgttcatg atgccccaga ccgtaccaaa cagaccaacg aacggagaaa tcgcgccgat
    40681 ggttgccaga tagccgttac cgcggcccat ttgacgaccc actgcggcga cccgacgctc
    40741 cagacggaag ctggtacgtt ctttaatacc ttcgttatcg tcgctgcctt ctgacagttc
    40801 cagctcattc tgcgcttcat tgagcaaatg caggcttaag cttttgctac caaaatcagc
    40861 ggcgatatcg ttggcctggt ttaaggaacg tgcttcagcc agcagttgct gctcgcgctt
    40921 aaggcgacgc ttctgattga agaactctac gctcttactg aagaagattg cccaggtgac
    40981 tacagaggcc aaaataagcc caatcatcac gcacttaacg acaatatcgg cgtactgata
    41041 cataccccag acggaaaggt ccgtctgcat taaattatta cccactgtgt atctccagga
    41101 cgcaagtcac aaaatctgcg cataataata tcaaaacgac gtcgaattga tagtcgttct
    41161 cattactatt tgcatactgc cgtacctttg ctttcttttc cttgcgttta cgcagtaaaa
    41221 aagtcaccag cacgccattt gcgaaaattt tctgctttat gccaattctt caggatgcgc
    41281 ccgcgaatat tcatgctagt ttagacatcc agacgtataa aaacaggaat cccgacatgg
    41341 cggacaaaaa gcttgatact caactggtga atgcaggacg cagcaaaaaa tacactcttg
    41401 gcgcggtaaa tagcgtgatt cagcgcgctt cttcgctggt atttgagagt atggaagcca
    41461 aaaagcacgc gacgcgcaat cgcgccaatg gcgagttgtt ctatggacgg cgcggaacgt
    41521 taacccattt ttccttacaa caagcgatgt gtgaactgga aggtggcgca ggctgcgcgc
    41581 tatttccctg cggggcggca gcggttgcta attcgattct tgcttttgtc gaacagggcg
    41641 atcatgtgtt gatgaccaac accgcctatg aaccgagtca ggatttctgt agcaaaatcc
    41701 tcagcaaact gggcgtaacg acatcgtggt ttgatccgct gattggtgcc gatatcgtta
    41761 agcatctgca gccaaacact aaaatcgtgt ttctggaatc gccgggttcc atcactatgg
    41821 aagtccacga cgttccggcg attgttgccg ccgtgcgcag tgtggtgccg gatgccatca
    41881 ttatgatcga caacacctgg gcagccggtg tgctgtttaa ggcgctggat tttggcatcg
    41941 atgtttctat tcaggccgcc accaaatatc tggttgggca ttcagatgcg atgattggca
    42001 ctgccgtgtg caatgcccgt tgctgggagc agctacggga aaatgcctat ctgatgggcc
    42061 agatggtcga tgccgatacc gcctatataa ccagccgtgg cctgcgcaca ttaggtgtgc
    42121 gtttgcgtca acatcatgaa agcagtctga aagtggccga atggctggca gaacatccgc
    42181 aagttgcgcg agttaaccac cctgccctgc ctggcagtaa aggacacgaa ttctggaaac
    42241 gagactttac aggcagcagc gggctatttt cctttgtgct taagaaaaaa ctcaatgatg
    42301 aagagctggc gaactatctg gataacttca gtttattcag catggcctac tcgtggggcg
    42361 ggtatgaatc gttgatcctg gcaaatcaac cagaacatat cgccgccatt cgcccacaag
    42421 gcgagatcga ttttagcggg accttgattc gcctgcatat tggtctggaa gatgtcgacg
    42481 atctgattgc cgatctggac gccggttttg cacggattgt ataacattgc cacttttgga
    42541 caattttgca gacattttat tgtgaaaagt cttaaattgt tgcgtccggg atcaaggcgt
    42601 cccggacgat tcaggagtac aataggcaga taaaggctta aacgctgttc cacaggaaag
    42661 tccatggctg ttattcaaga tatcatcgct gcgctctggc aacacgactt tgccgcgctg
    42721 gcggatcctc atattgttag cgttgtttac tttgtcatgt ttgccacgct gtttttagaa
    42781 aacggcctgc tgcccgcctc atttttgcca ggcgacagct tgttgatact ggcaggcgca
    42841 ttgattgccc aaggggttat ggattttctg cctacgattg cgattctgac cgccgcagca
    42901 agtctgggct gctggctaag ttatattcag gggagctggt tagggaatac caaaacggtg
    42961 aaaggctggc tggcacagct tcctgctaaa tatcaccagc gcgccacctg catgtttgac
    43021 cgccacggtc tgctggcgct gctggctgga cgttttcttg catttgtccg tacgctgctg
    43081 ccaaccatgg cgggaatttc cggtctgcca aaccgccgct tccagttttt caactggtta
    43141 agtggattgc tgtgggtcag cgtggtaacc agttttggct atgccttaag tatgattccg
    43201 ttcgttaaac gccatgaaga tcaggtaatg acgttcctga tgatcctgcc aattgccttg
    43261 ttaaccgctg gcttgttagg cacgctgttt gtggtgatta aaaaaaaata ctgtaacgcc
    43321 tgacgatttt ccccgttccc ggctgctgta ccgggaacgt atttaattcc cctgcatcgc
    43381 ccgcattctt gccgcatctt cccccggcgt cacaccgaag taacgtttaa actcacggct
    43441 aaattgcgat gcgctttcat agccgacgcg catcgctgct gcgctggcct tcatgccgtc
    43501 atggatgatc atcatccgcg ccttatgcag acggtaattc ttcaaatact gcaacggcga
    43561 ggtgctggtg acagacttaa aattatggtg gaacgccgat acgctcatgt tggcttctgc
    43621 cgccagttgc tcgacgctca ggttttcggt gtatttattc tcaattcgtt tcagcacgcg
    43681 gctaatcaga ctgaagtgag tctggcgact gaccagcgcc agtaacgcgc cgccgcaagg
    43741 tccggtcagc acgtagtaca gaatttcgcg gatgatctgt ttgccgagaa tacgcgcatc
    43801 cagtggtcgc tccatcacgt cgagtaaccg ctccgccgcg cataaaatct cttctgataa
    43861 cgtggcggag ttaatcccgc tggctgccat cgacggctgg aaatgctcat cttcgccaat
    43921 gtccatcaac agttcctgta actgcaaaat atcgacattg agacgcaacc ctgccagcgg
    43981 cacctctgac gtggcatagg tttcgcactc aaacggcaac ggcaccgtca gcagcaggta
    44041 ttcattggca tcataacgaa acacgcgttc attgatataa ccgattttat gcccggaaaa
    44101 gagaattatg atgccaggct cgtacatcac cggtgtacgt gcgaaaggcg tctcgccata
    44161 caacaaacgc acatcgggca acagttctga caaactattt tctttatttt tcagtttatt
    44221 aactttatcc gccagcaagc gacaaatctc ttcacgtttc atatcgcgta atttcttagg
    44281 aataatgcgg caatttgatt gtgcgcaatt ttgtagcatt tctccagcac tctggagaaa
    44341 taggcaagac attggcagaa atgagcattg agagccaggg cgctggcgat cacaatgaaa
    44401 aacatcaggc agatcgttct ctgccctcat attggcccag caaagggagc aagtaatgaa
    44461 caactttaat ctgcacaccc caacccgcat tctgtttggt aaaggcgcaa tcgctggttt
    44521 acgcgaacaa attcctcacg atgctcgcgt attgattacc tacggtggcg gcagcgtgaa
    44581 aaaaaccggc gttctcgatc aagttctgga tgccctgaaa ggcatggacg tgctggaatt
    44641 tggcggtatt gagccaaacc cggcttatga aacgctgatg aacgccgtga aactggttcg
    44701 cgaacagaaa gtgactttcc tgctggcggt tggcggcggt tctgtactgg acggcaccaa
    44761 atttatcgcc gcagcggcta actatccgga aaatatcgat ccgtggcaca ttctgcaaac
    44821 gggcggtaaa gagattaaaa gcgccatccc gatgggctgt gtgctgacgc tgccagcaac
    44881 cggttcagaa tccaacgcag gcgcggtgat ctcccgtaaa accacaggcg acaagcaggc
    44941 gttccattct gcacatgttc agccggtatt tgccgtgctc gatccggttt atacctacac
    45001 cctgccgccg cgtcaggtgg ctaacggcgt agtggacgcc tttgtacaca ccgtggaaca
    45061 gtatgttacc aaaccggttg atgccaaaat tcaggaccgt ttcgcagaag gcattttgct
    45121 gacgctaatc gaagatggtc cgaaagccct gaaagagcca gaaaactacg atgtgcgcgc
    45181 caacgtcatg tgggcggcga ctcaggcgct gaacggtttg attggcactg gcgtaccgca
    45241 ggactgggca acgcatatgc tgggccacga actgactgcg atgcacggtc tggatcacgc
    45301 gcaaacactg gctatcgtcc tgcctgcact gtggaatgaa aaacgcgaga ccaagcgcgc
    45361 taagctgctg caatatgctg aacgcgtctg gaacatcact gaaggttccg acgatgagcg
    45421 tattgacgcc gcgattgccg caacccgcaa tttctttgag caattaggcg tgccgaccca
    45481 cctctccgac tacggtctgg acggcagctc catcccggct ttgctgaaaa aactggaaga
    45541 gcacggcatg acccaactgg gcgaaaatca tgacattacg ttggatgtca gccgccgtat
    45601 atacgaagcc gcccgctaag ctttttacgc ctcaaacttt cgttttcggg catttcgtcc
    45661 agacttaagt tcacaacacc tcaccggagc ctgctccggt gagttcatat aaaggaggaa
    45721 cgtatggcta atccaaccgt tattaagcta caggatggca atgtcatgcc ccagctggga
    45781 ctgggcgtct ggcaagcaag taatgaggaa gtaatcaccg ccattcaaaa agcgttagaa
    45841 gtgggttatc gctcgattga taccgccgcg gcctacaaga acgaagaagg tgtcggcaaa
    45901 gccctgaaaa atgcctcagt caacagagaa gaactgttca tcaccactaa gctgtggaac
    45961 gacgaccaca agcgcccccg cgaagccctg ctcgacagcc tgaaaaaact ccagcttgat
    46021 tatatcgacc tctacttaat gcactggccc gttcccgcta tcgaccatta tgtcgaagca
    46081 tggaaaggca tgatcgaatt gcaaaaagag ggattaatca aaagcatcgg cgtgtgcaac
    46141 ttccagatcc atcacctgca acgcctgatt gatgaaactg gcgtgacgcc tgtgataaac
    46201 cagatcgaac ttcatccgct gatgcaacaa cgccagctac acgcttggaa cgcgacacac
    46261 aaaatccaga ccgaatcctg gagcccatta gcgcaaggag ggaaaggcgt tttcgatcag
    46321 aaagtcattc gcgatctggc agataaatac ggcaaaaccc cggcgcagat tgttatccgc
    46381 tggcatctgg atagcggcct ggtggtgatc ccgaaatcgg tcacaccttc acgtattgcc
    46441 gaaaactttg atgtctggga tttccgtctc gacaaagacg aactcggcaa aattgcaaaa
    46501 ctcgatcagg gcaagcgtct cggtcccgat cctgaccagt tcggcggcta acgtgcaaat
    46561 tctcccggtg gcggtaatgt tccgctaccg gacttttcag aaatcattta ttcccctcgc
    46621 gtcccgcccg ttgttactct tccttgttca ggaatgccaa atataaggac atcatcatgc
    46681 agagccggaa gctcttaaaa gaacaactca tctatatccg ggataaacgc aacggagagg
    46741 tgaaaaacag atgaaaataa tacttctgtt tttagcagcc ctggcaagtt ttaccgtaca
    46801 cgcgcagcct ccttcacaga ccgtagaaca aacagtccgg cagatttatc agaactataa
    46861 atcagatgcc agtacgcctt atttcggtga aaccggagag cgggcgataa cttcagcgcg
    46921 tattcaacag gcgcttaccc tgaacgacaa tcttacacta ccgggcaata ttggctggct
    46981 ggattatgat ccggtttgtg attgtcagga ttttggcgat ctggtgttag aaagcgtagc
    47041 gataacccaa actgacgtcg atcatgccga tgccgttgtg cgctttcgta tctttaaaga
    47101 tgataaagaa aagaccacac agacactgaa aatggtggcg gaaaatggtc gttgggtcat
    47161 tgatgatatt gtcagcaatc atggcagcgt cttacaagca gttaatagcg agaatgaaaa
    47221 aacgctggcc gctttagctt cgttgcagaa agaacagcca gaatcctttg ttgctgaact
    47281 ctttgaacat attgctgatt atagctggcc gtggacgtgg gtggtttccg actcttaccg
    47341 ccaggcggtt aatgccttct ataaaaccac cttcaagacg gccaataatc ccgatgaaga
    47401 tatgcaaata gaacggcaat ttatttacga caatccgatc tgttttggcg aagagtcgct
    47461 attttcacgc gttgatgaaa ttcgagtcct ggagaaaacc gccgattccg cccgcattca
    47521 tgttcgtttt acgctgacca atggcaacaa tgaagagcaa gagctggttt tacagcggcg
    47581 cgaaggtaag tgggaaatcg ctgattttat ccgcccgaac agcggcagcc tacttaagca
    47641 gattgaggca aaaaccgccg ccagattaaa gcaatgagct gaattaaata acaattagcc
    47701 ggaacaataa ataaaaggga acactatatg aaaacgattt tcaccgtggg agctgttgtt
    47761 ctggcaacct gcttgctcag tggctgcgtc aatgagcaaa aggtcaatca actggcgagc
    47821 aatgtacaaa cattaaatgc caaaatcgcc cggcttgagc aggatatgaa agcactacgc
    47881 ccacaaatct atgctgccaa atccgaagct aacagagcca atacgcgtct tgatgcacag
    47941 gactattttg attgcctgcg ctgcttgcgt atgtacgcag aatgataaaa aaatccccgg
    48001 cagcatgtca gttgccgggg atttttttta acgtccaacc gccgctttag ggcgtttctt
    48061 cgcaccagca ttcaccggac gagattgcgt agacgacgct ttttttgccg tagcaggcgt
    48121 ctgacgctga gtcgccatcg gcgtatgttt agtcaacgcc ggacgggtat tgcggttctg
    48181 gcgacgagct tcacgcatct cttcaatggt tggcgcaggc actaagcaat cgcgacggct
    48241 gccaatcaga tgctttttgc ccatcgcttc cagcgcctgg cggattaacg gccagtttgc
    48301 cggatcgtgg taacgcaaca acgctttatg caaacgacgc tgtttgtcgc ccttcggtac
    48361 gaagacgcct tcgctcttat aaccaatctt cgccagcggt tttttcccgg tgtaatacat
    48421 ggtggttgag ttcgccagcg gcgatgggta gaagttctgt acctggtcga gacggaaacg
    48481 atgctttttc agccacagcg ccaaattcac catatcttca tcacgcgtac cggggtgcgc
    48541 agagatgaaa tacgggatca gatactgctc tttacctgcc tgtttcgagt aggtatcgaa
    48601 cagctcttta aagcggtcat agctgcccat gcccggcttc atcatcttcg acaacggccc
    48661 ttcttcggta tgttccggag caatcttcag ataaccgccg acgtgatggg tcgccaactc
    48721 tttgatatag cgcggatctt ccacggcgat gtcataacgt acgcctgagg cgataaggat
    48781 ttttttaatg cctttcagat cacgcgcacg gcgatagagg ttgatcgtcg gttcgtggtt
    48841 agtgtccatg tgcggacaaa tatccggata aacgcacgac aaacggcgac aagtttgttc
    48901 agcgcgtggc gatttgcagc gcaacatata catgttggca gttggcccac cgagatcgga
    48961 aatcacgccc gtaaaacctg gaacggtgtc gcggatcgct tcgatctcat taatgatcga
    49021 atcttccgaa cggctctgaa taatgcgccc ttcgtgctcg gtaatagaac agaaagaaca
    49081 gccgccaaag cagccgcgca taatgttgac cgaaaaacgg atcatttcgt aagccggaat
    49141 acgggcattg ccataggccg gatgtggcac gcgcttgtac ggcagcgcaa aaacgctgtc
    49201 catctcttcg gtagaaagcg ggatagcagg cgggttgatc cacacatagc ggtcaccgtg
    49261 tttttgcatc aatgcgcggg cgcagcctgg gttggtttcg tggtgcagaa tgcgcgaagc
    49321 atgggcgtac agcactttat cgcccttcac tttctcgaaa gaaggcagca atacgtaggt
    49381 tttttcccac ggtttctggc gcggtggctg cacgattaca gctttcgctt cctgcttttt
    49441 cggcgcaaca ggtttgttat ccgcacacgg caaatcttcg ccatacggat gcgggattgg
    49501 gtcgattttc cctagggtat caagacgggt ggaatccaca ccgctccagc caggcagcgc
    49561 ctcttttacg ataattgcgg tattacgcac atcgcggatt tcactaatcg gctcgcccat
    49621 cgccagacga tgcgctactt ccaccagcgg acgttcaccg ttaccaaaca tcagcatgtc
    49681 ggctttcgaa tctaccagaa cggaacggcg cacggtatcg gaccagtaat cataatgcgc
    49741 ggtacggcgc aggctagcct caataccgcc gaggatcacc ggcacatctt tccacgcctc
    49801 tttacaacgc tgggtataaa ccagcgtggc gcgatccggt cgcttacccg cgacattatc
    49861 tggcgtgtag gcatcatcat gacgtaaacg gcgatcggcg gtatagcggt tgatcatcga
    49921 atccatgttg ccagcagtaa caccaaaaaa cagattcggt ttacccagac gcataaagtc
    49981 gtctttgctg ctccagtccg gctgggcgat gatcccgacg cgaaagccct gcgcttccag
    50041 catacgaccg caaatcgcca tcccgaagct tgggtgatcg acatacgcgt cgccagtaac
    50101 caaaatgatg tcgcagctat cccagccaag ttgatccatc tcttcacgag acatcggcaa
    50161 aaacggtgcc ggtccaaaac aggcggccca gtactgcggc caggagaaca ggtcgcgatc
    50221 cggttggatc agggagatag agctcatttt gcttccagaa atgataaaaa aataatcaaa
    50281 ggccggggat tataagccgg aacgaaagag aaatcgaaag gtattccata ctcgccctcc
    50341 tcgggcgagt atgaagatta cggtaccgga ttgaccaaca gttgcccaat cgagccacgg
    50401 tccgccattt ccagcgtctg actgttgaag tagaacggga agtgcgccca ggaaggctga
    50461 ccgaaataaa caagcagctc cacttgtccg tcaacccaaa cggtatcttt ccagcctctg
    50521 tcttccggga acggcatcgc gccattcacg ttacggatct ggaacattac gccttcaata
    50581 tggaacgctt gcggctcgtc cgcgcgtacc gtccagcgtt cccacgttcc ttgctgcgcg
    50641 gtgacatcaa tacggttgac gtcccacagc tgtccattaa tacccgggtc atcacccaga
    50701 ctgatatcgc gactgcgaat tggcgaacca gccatgattt cagttggcag caagcgcatc
    50761 ggaagactgt ctgtgaccag cggcagaagg ccggttgggc gcagcgttag caccaaggta
    50821 gaaaccagaa tactggatgg ctcaaagaag ccgcgaatac gatcaacaat gctcgccgct
    50881 tcaccacagg tgatcgacac ttcatcgccg ttgctcatat ccaccagaat ctcgcggcgc
    50941 tcgcccggtg ccagcgaaag ttgcttcacc gatacaggag caggcaggaa tccctgatcg
    51001 ccagaaatca catgtaacgg gcgaccatcg ctcatctgta gttgatagcg acgagagttc
    51061 gacgcgttca gcagtcgcaa gcgcacccag ccacgcgaga cttcaacgta cgggctttgt
    51121 acaccgttaa ccagcagcgt atcaccaaca aagccgccgc ttcccggttc gttgtattct
    51181 ggcgtaccaa agttatccag ccgtttatcc tggataatga ccggaaaatc atccacacca
    51241 taatggttgg ggataggcag cgacttgctg acttcatctt ccaccagcca cattccggca
    51301 aggccgttat agacctgctg agccgtgcgg ttgggagtat tggcgtgata ccacagagta
    51361 gctgcgttct ggcgaatagg cagtacgggt gcccagtcag cgtttggcga catcatccgt
    51421 gccggaccgc ccatcagcgg gcctggtacc tgtagcccgg ccaccgtcat tgagacattt
    51481 tctgtcaggc ggttgctgta aataagctta acatcgtcgc ccttccagac gcggatagtc
    51541 ggccccaggt aacgaccatt gattccccag accgacgcgc gtgtccctgg cgtaaatgac
    51601 cagtgcgcac gttgtacagt cataaacagc ggttgcccac ggcgagattc aagtagcggc
    51661 ggaacgggta gcggttgctg ttgcccggct gcgctggcct tcaggggaac agcgcctgca
    51721 caaagtgcaa tccccgatgc ctgaatgaac tgacgccgac tgagtgacat atttgctcca
    51781 tgtaaaactg gctaaagtaa taccgtgcag gaaagtgttc cccgcaaaaa tacagattgc
    51841 cgacttaaac ttttccggcg gcttcgcgtt ctgcgacttc tttatcgagc tcggcgattt
    51901 tttgttccat tatcgaacga caatgggcag ccagctcacg aacctgatct ttgccatact
    51961 gactgacgtc aattggcggc agcatttcga caatcaccag accgttgtgc agtcgattaa
    52021 gattaatctt attcgaagtt gtagagacgc acacgggaat aatcgggacg cccgccgcaa
    52081 ttgccgcgtg aaatgctcca gtcttgaacg gtagcaggcc gcgaccacgg ctgcgggttc
    52141 cttccgggaa catccagatg gaaatacggc gttttttgaa atgattcacg acttccgcaa
    52201 tggtgccgtg agctttagtg cgattgtttc tgtcgatcaa taagttgccg gttaaccagt
    52261 acaactgccc gaagaagggg atccacagca agctcttttt acctaccgtc accgtcggcg
    52321 gttgcacaat gttcgatgct gtcaccatgt catagttgtt ctggtggtta gcgatataga
    52381 tagcattgcc gtagctttca gcatctgcag gtttgcggca ctcaactttc aggccgaaca
    52441 gtggcgcaag acggccaaac atatgcccaa aggtggccac atgtttcggg ttacgcgggc
    52501 tgaaaaggca gtaaatggag ccgaatacac agactaagat gctgtaaatc acggtaataa
    52561 taagacgaaa gatatatagc ataacaccct ctgaaggtct gagagactgg cattgtacgt
    52621 cacctgaaat caggttaggg cattgttaaa agccgctctc tggaaaagcg tattgttaat
    52681 cgcaacgcat gaataaacaa cggtttcaag ggaattttta tcgtttccgg gggttgaata
    52741 cgctgccgga tgacgactta acgtttcatc cggcgttcct tgctagcggg aggaaacagc
    52801 gccctccccg gcatattact cttcgctatc accgctgctg gcacggcgag gagagtcgat
    52861 ctcaacacga tcgatacgct gcaaaccgcg catcaacgta ccgcggcgtc cacgttcgcc
    52921 agtgactttc tgtagctctt ccggacgcag tttaattttg cgtttcccaa catgaatggt
    52981 cagcgtgctt tgcggcggca gaacgtacag ttgcgccaga ccatcctcgc cacgcgcggc
    53041 ttctgccgat ggaatgttga taatcttgtt gcctttgccc ttcgacagct gcggcagatc
    53101 gcttaccggg aacatcaaca tacggcctgc ctgagtgatt gccagcagca tatcggaagc
    53161 atcttcaatc accaccggcg gcataacatg ggcattttcc ggtaaggtga tcaaagcctt
    53221 acccgcacgg ttacgcgcta ccagatcgtt aaaggtgcag acgaaaccgt aacccgcatc
    53281 ggaagccatc agcagtttct gatcgtcgct ttccatcagc atatggtcaa cggtcgcccc
    53341 aggcggcaac gttaatttgc cggtgagtgg ttcgccctga ccacgcgccg acggcagcgt
    53401 aatcgggtcg atggcatagc tacgaccggt ggaatcaaca aacactaccg gttggttact
    53461 cttacctttc accgccgctt tgaagctatc acccgcttta taattcaggc ccggcgcgtc
    53521 gatatcatgg cctttagcgc tgcgtaccca gcccatctgc gacagcacaa tggtgacagg
    53581 ttcagacggc agcatgtcgt gctcgctcat cgctttcgct tcttcgcgtt cctgcaacgg
    53641 cgaacgacga tcgtcaccgt aggcttgcgc gtctgcctgc agttctttct tcagcaggtt
    53701 attcatttta cgctcggaag ccaaaatgcc ctgcaactgg tcgcgctctt tttccagctc
    53761 actctgctca ccgcgaatct tcatctcttc cagtttggca agatgacgca gtttcagttc
    53821 gaggatcgct tccgcctggg tttccgtaag gccaaaccgc gacatcagcg ccggtttcgg
    53881 ttcatcttcg ttacgaatga tctcaatcac ttcgtcgatg ttgagaaacg ccaccagcaa
    53941 accttccagg atatgcaggc gcttgaggac tttctccaga cgatagttca gtcggcggcg
    54001 cacggtatcg cgacggaaca ccagccattc ggagaggatt tccagcaggt ttttcaccgc
    54061 cggacgacca tccagaccga tcatattgag gttaatgcga tagctctttt ccagatcggt
    54121 ggtagcgaag aggtggttca tcacctgatc catatccacg cggttggaac gcggcacaat
    54181 caccagacgg gtcgggttct cgtggtcaga ttcatcgcgc agatcgtcaa ccatcggcag
    54241 ctttttgttg cgcatttgcg cagcaatttg ctccagtacg cgcgcacctg aaacctgatg
    54301 cggtaatgcg ctgataacca ccgcgccatc ttctttcttc cacaccgcgc gcatacgcac
    54361 tgaaccacgt ccgttctcgt agattttacg aatctcggcg cgcgaagtga taatttccgc
    54421 ttcagtcgga taatccggcc cctgcacgat atccagcagc tgatcgagcg tagttttcgg
    54481 ctggtcgatt aatgcgattg ccgcctgagc tacttcacgc aggttatgcg gtggaatatc
    54541 ggtcgccatg ccgacggcaa taccggtggt gccgttaagc aaaatgtttg gcagacgggc
    54601 aggtagcatt ttcggctcct gcaaagtgcc gtcgaagttt ggcacccagt cagccgtccc
    54661 ctgccccagc tcgctcaata gcagctcgga atatttcgac aaccgggatt cggtgtaacg
    54721 cattgccgcg aacgatttcg gatcgtccgg cgcgccccag ttcccctgac catcaaccag
    54781 cggataacgg taagagaacg gctgcgccat caggaccatc gcttcataac aggcgatatc
    54841 gccgtgcgga tggtatttac ccagtacgtc accgacggta cgggccgatt ttttaaattt
    54901 ggcgctggca ttcaggccca gttcagacat cgcatacaca atgcggcgct gaacaggttt
    54961 cagaccatca ccaataaacg gcaacgcacg gtccatgatc acgtacatgg agtagtttaa
    55021 gtaggcgttt tccgtaaatt catgtagcgc aaggcgctct gccatatcgc tcattaattc
    55081 tgattcctca acttattcgc cagccttcaa aggggcaata ttgccggaga tactacatta
    55141 tctgccgcgt cgagtcacag gagatcagcg tgaatagcgc cgccggacgt gctccgacgg
    55201 cgataaaggc atcaatgtgc cttgatatac aactctttgc tgtagacttg atcggcatag
    55261 agggcagcac aaggttgcgc ccatgccaca ccactcggca tgggggtccg ctccgggcga
    55321 ttcgagaagt tgaggttatg cgagtccagg gaagcctaaa gcatcaaagt agtcgactgt
    55381 aagtatacgg ttcagcccag tttagctgtg tctccaccag tgccccgcgc ctcctgccat
    55441 aagtgcggct tgctcatgga tatccccgag acggcttacg gcgcaccgga taataaattt
    55501 cgcgtataca tggtattcct taccggagaa attataagcc caacaacaat acgcctgtga
    55561 cattgttcgc tcaaacttat cgcgagtgat ttggctcaca ctccggtaaa gattccccat
    55621 cacgccaacg gcacataaac atccgtttcc agtcgctgct ctgtcacccc ttcggcaaga
    55681 ttggtgtagt gaaacagaat cggtgcttta cgcattttct cgccgcttgc aggcagccag
    55741 tggcgaataa tgccccatat cgtgtgtgaa atatcgtcca gctcgccaac gtggcgggcc
    55801 acggcataac gtccaccggt aagttcacca ttgctcacac cataacgatt atcgggaatc
    55861 ggttcgctaa cgctgccgca gatatcaaag cgaaacgctt ccggtgcggt ggtggcaggg
    55921 gcgtcccagg cgacgccaaa agtctggctt tggttaacag gagaaagccc cgtctcttta
    55981 cgccacatga taaatttcgc cacactgtag ttgagcaact cagggctaca gcggtgcggc
    56041 aacatcgcca cagggattga tgggaaatcg ataatgttta catccagtgt caggtttgtc
    56101 attttgcctc cctgcgaaat tattcccggg aacgcgtccc gggagcggta acaattacgg
    56161 atttactttg cggatctgtt tgacgtcaat ttcaacagaa ttccagtctt tatcgacttc
    56221 accctgaatc tcaaccgtat ctttcggcgt caccgtcaca ccgttccagc gtttgtgatc
    56281 gatatcaaca ttgatagtac cgctggcatc tttaaacacg tagagatcgt cagagatgcg
    56341 ttcaacgata ttgccgcgca gggttaccca ggtgtcgtca cgcagggatt ttgcgctttc
    56401 tacagtcgtt acgctgccgt tcggcccctg aaatcctccg gcctgacttt gcgttaccga
    56461 tgggccagaa aaaccgccct gctctgctgc catcaccggt gcactgcaca gggccattac
    56521 tgcgattact gctgcgaatt ttttcatgtt tattactccc tttaatgtct gtttccgagc
    56581 atttaacaag atagtcctta acaacttctt aagggaaaaa aataaaattt aatgctgtac
    56641 agagcgcgtt acaacacggt ttactggcag caaatacggt tatcgcaggg atgaaaaaat
    56701 gcgaatttta ctgatagaag atgacatgct gattggtgac ggcatcaaaa ctggccttag
    56761 taaaatgggt tttagtgtcg actggtttac acaaggtcgt cagggaaaag aggcgcttta
    56821 tagcgcacct tatgatgcgg tgatcctgga tttaacctta ccaggcatgg atggtcgcga
    56881 tattttgcgc gaatggcgag agaaaggtca gcgtgagccg gtactgaacc tgaccgcgcg
    56941 tgatgcgtta gcggaacgtg tagaagggct gcgtctggga gctgacgatt atctgtgtaa
    57001 accttttgcg ttgatagaag tcgccgccag gctggaggct ctgatgcgcc gaaccaacgg
    57061 ccaggccagc aacgagctgc gccacggtaa cgtcatgctc gaccccggca aacgtatcgc
    57121 cacgctggct ggcgaaccct taacactgaa accaaaagaa tttgccctgc tggaattact
    57181 gatgcgtaac gctggtcggg tactgccgcg caaactgatt gaagagaaac tgtatacctg
    57241 ggacgaagag gtcaccagta atgccgttga agtgcatgtg catcatctgc gacgcaaact
    57301 cggtagtgat tttattcgta ccgtgcatgg tattggctac acattaggtg agaaatgaaa
    57361 tttacccaac gtcttagtct gcgcgtcagg ctgacgctaa tctttttaat tctggcctcg
    57421 gtgacctggc tgctttccag ctttgtcgcc tggaaacaaa caacggataa cgtcgatgaa
    57481 ttgttcgaca cccaactgat gctgtttgcc aaacggttaa gtacgctcga tctcaacgaa
    57541 atcaacgcgg cggatcgcat ggcacagacg ccaaataaat taaaacacgg tcatgttgat
    57601 gacgatgcgc tgacctttgc catctttacc cacgacggca gaatggtcct taatgatggc
    57661 gataacggag aagatattcc ctatagctat caacgggaag gttttgctga cgggcaactg
    57721 gtcggtgaag acgatccttg gcgttttgtc tggatgacct cacctgatgg caaatatcgc
    57781 atcgttgttg gccaggaatg ggaataccgt gaagacatgg cgctggcgat tgttgccggg
    57841 caattgatcc cgtggctggt cgcactgccg attatgttaa tcatcatgat ggtactactg
    57901 ggtcgtgaac tcgcgccgct gaacaaactg gcgctggcac tacgtatgcg tgaccctgac
    57961 tcggaaaaac cactaaacgc gactggcgta cccagcgaag tgcggccact ggttgagtcg
    58021 ctaaatcaac tgttcgcccg cacacatgcg atgatggttc gagaacgacg ctttacctcc
    58081 gatgcagctc acgaactacg tagcccgtta acagcgctga aagtgcaaac cgaagttgcg
    58141 cagctctctg acgatgatcc gcaggcgcgg aaaaaagcac tgctccaatt acattccggg
    58201 atcgatcgcg ctactcgtct ggttgatcaa ctgctcacgc tatcgcggct ggactcactg
    58261 gataaccttc aggacgtcgc ggagatcccg cttgaagatc tcctacaatc gtcggtgatg
    58321 gatatttacc acacggcgca gcaggcgaaa attgacgtgc gactgacact caatgcccac
    58381 ggcatcaaac gcaccggaca accgctattg ctaagtttgt tggtgcgaaa tttgctggat
    58441 aacggccccg gtgtgacacc agaggcactg gcgcgaattg gcgaacgctt ctatcgccca
    58501 cccggacaaa ccgctaccgg cagtggactt gggctatcga ttgtccagcg aatcgccaag
    58561 ctgcatgaca tgaatgttga gtttgggaat gcggaacaag gtggatttga ggcgaaggta
    58621 agctggtaac ttctctgtct atgagagccg ttaaaacgac tctcatagat tttactaata
    58681 gcaaaatata aaccgtcccc aaaaaagcca ccaaccacaa accagacaaa taacgagcga
    58741 gaatacatat agccaaaact cgaacatttc ccgcataaag agttttctta agataagaat
    58801 aataagtggc gtaagaagaa aaaatgctgc aatcattgct tgcaatatac gatcttcgcc
    58861 tgaaacggaa ggtatactgc aaatattgcc tcccgactca agcatccagt catacttatt
    58921 gaaagcaaaa aaagaatcta ttaaccaata agtaagcatt atatatggca atgcttcaaa
    58981 tattgtttta attttctgct ttaacatata tcagtacgct cataaaaatc catttataat
    59041 tatcaacatg acacgttgat attttcatct tttacttatt caaagccata ttttcaatcc
    59101 ccaaaaaaac cgaaccaaac gtgtaaatta ttccggtgcg agtaagaatt atgaggaatg
    59161 gctatcagta ttgtcatttt cagaaaatat ttatcctgca tcggtgagtc agagctagat
    59221 cagacttttg ctaaattcgc aaaagacttt gcacattttg ctaatttcac agtaccgctc
    59281 tgtgacgtac tatagtcgac aaacgtctca ccttgagatt aaaaaatgag caacatcctg
    59341 attatcaacg gcgcgaaaaa attcgcccac tccaatggtc aactgaacga caccctgacc
    59401 gaagtcgcgg atggcacact gcgcgacctc gggcatgatg tccgcatcgt tcgcgccgac
    59461 agcgactaca atgtcaaagc ggaagtacaa aactttctct gggctgatgt ggtgatctgg
    59521 cagatgccag gctggtggat gggcgcgccg tggacagtga aaaaatatat tgatgatgta
    59581 ttcaccgaag gtcacgggac tctgtatgcc agcgatggtc gtacccgcaa agatccgtcg
    59641 aaaaaatacg gttccggcag cctggtacag gacaaaaaat atatgctttc tctaacctgg
    59701 aacgcaccaa tggaagcctt caccgaaaaa gatcagttct tccacggcgt tggcgttgac
    59761 ggtgtgtatc tgccgttcca taaagcaaac caattcctcg gtatggaacc gctgccgaca
    59821 tttatcgcta atgacgtgat aaaaatgcct gatgttcccc gctatactga agaatatcgc
    59881 aagcatcttg tggaaatttt tggttaacta gagctcaggc tttagaagga gttaaccatg
    59941 cttaccgtaa tcgcagaaat ccgtactcgt cctggtcaac atcaccgtca ggcggtattg
    60001 gatcagtttg ctaaaatcgt tccaaccgta ctgaaagaag aaggttgcca cggctatgcg
    60061 ccaatggtgg attgcgctgc tggcgtgagt ttccagtcta tggcaccgga ttctatcgtg
    60121 atgattgagc agtgggaaag catcgcgcac cttgaagcgc atctgcaaac cccgcacatg
    60181 aaggcgtaca gcgaagccgt aaaaggcgac gtgctggaga tgaatatccg tattctgcag
    60241 ccagggattt aatcctgcct tgtttgcccg gccatcctga ccgggcaatg ttctttcctt
    60301 taaacctcaa tctccgccat gtcgcctttc tcttgcaacc agttgcggcg atcttccgag
    60361 cgtttcttcg ccagcagcat atccatcatc gcgtcagtac gctgatcgtc ttcatcatcg
    60421 atagtcaact gcaccagacg gcgagtgttc ggatcaagcg tggtttcacg caattgcatc
    60481 gggttcattt cccccagacc tttaaaacgc tggacgttcg gcttgccttt cttgcgtttt
    60541 aattgctcaa gtacgccctc tttctcttct tccgtcagcg cgtaataaac ctctttcccg
    60601 agatcaatac ggtagagcgg tggcagtgcg acgtaaacgt gaccgtgttt caccaacgcg
    60661 cggaagtgtt ttacgaacaa agcgcagagc agcgtggcaa tgtgcagacc atcagagtcc
    60721 gcatccgcga ggatacagat tttgccataa cgaagctggc tcagatcgtc gctgtcagga
    60781 tcgataccga tcgctaccga aatatcatgc acttcctgcg aggccagcac ttcgtcggaa
    60841 gaaacttccc aggtgttaag gatcttacct ttcagcggca tgatcgcctg atattcacga
    60901 tcgcgcgcct gcttggcaga tccgcctgcg gagtcacctt ccacgaggaa cagttcggta
    60961 cggttaaggc cctgcgcggt acaatcagcc agtttgccag gcagcgccgg gccgctggtc
    61021 agctttttac gcaccacttt tttagccgca cgcatacgac gctgggcgct ggaaatcgcc
    61081 atctctgcca gcaactccgc cgcctgaacg ttctggttca gccacaggat gaaggcatct
    61141 ttcaccacgc ccgaaacgaa tgccgcacac tgacgcgaag agagacgctc tttggtctgc
    61201 ccggcaaact gcggatcctg cattttaacc gacagaacat aggcgcagcg atcccagata
    61261 tcttccgccg acagcttcac gccgcgcggc agaatgttgc ggtattcaca gaactcacgc
    61321 atcgcgtcca acaggccctg acgcagacca ttaacatggg taccgccctg catcgttggg
    61381 ataaggttga cgtagctttc ggtcagcagt tcaccgcctt ccggcagcca cagtagcgcc
    61441 cagtccacag cttcagtatc gccagcgaaa ttaccgataa acggtttttc tggcagcgtc
    61501 ggtaaaccgt ttaccgcttc cgccaggtaa tcattcagac cgtcctgata gcaccagcgc
    61561 tgttcggtgt tgttgatctc atctttaaag gtaatctcaa cgccggggca cagtactgct
    61621 ttggccttca gcacatgcgt caggcgtgaa acagaaaaac gcgggctgtc aaagaaggtt
    61681 tcatccggcc agaagtgcac gctggtaccg gtattgcgtt taccgcaagt gccgacaacc
    61741 tgtaaatcct gcaccttttc gccattttca aaggcgatgt tataaacctg accatcgcgg
    61801 cgcacgttaa cttctacgcg cttcgacaag gcgttaacta ccgaaatccc cacgccatgc
    61861 aggccgccag agaactggta atttttgtta gagaatttac cgcctgcatg cagacggcaa
    61921 agaatcagtt caaccgccgg tactccttct tccgggtgaa tatccaccgg catcccgcgc
    61981 ccatcgtcaa tcacttctaa cgactggtca gcatgtaaga ttacatccac gcgttttgcg
    62041 tgacccgcca gtgcttcatc gacactgtta tcaataactt cttgccccaa atggttaggg
    62101 cgagtggtat cggtatacat ccccggacgg cggcgaaccg gctcaagccc ggtgagtacc
    62161 tcaatggcat cagcgttata agtttgcgtc atggtttaag ttagcaattc gagttgatcg
    62221 tcagagatgg tgcagaccaa gaaaatcgac gatcgggttg aaataatctt cgaagcccgt
    62281 gaatgcgtgg ttgccgcctt ctatgacagt ctggcggcag gaagcatagt acgccaccgc
    62341 ctggcggtaa tccagcactt catctcccgt ctgttgcagc agccagatca aatccggcgc
    62401 ttccagcggg tcaatctgca tgactttgag atcgtaaata tggcgtgact ctagcacata
    62461 ttgctgcccg gtgtaggggt tctcgttctg accgagatag tccgtcagca attcaaacgg
    62521 gcgtaccgcc gggtttacca ccactgcggg cagcataaaa cattgcgaca accaggtggc
    62581 gtaatatccc cccagtgacg aaccgacaat acccagcgaa tcgccgccat gttccaggac
    62641 aatggattcc agcagttctg ccgcgtcgga aggatacggc ggcaactgcg gaatgatcat
    62701 ctcaacgtca gggtgatgtt ccgccagcca gttttttaac aagctcgctt ttgcagagcg
    62761 cggcgagctg ttgaaaccgt gtaaataaag aagcgtagac atcagtagcc ttctgaagcg
    62821 gtatcaggtt ggaaacgtgt gtccgccagg cgatgcacct cggtggtcag cgtgccatca
    62881 gcatgtaact cgagagtacg ccagccgggc gcgatagtat ccagcgtaaa gttggaacag
    62941 tgcggcttaa actgcacaca ggtcgacggc gttgccagca ggcggcgacc gttccagtcg
    63001 agatccagct cctgatgaat atgaccgcac agcaagtatt tgacgtgcgg aaacttcgcc
    63061 agcacggtat ccagttcgcc cgcgttacgc agactgtgtt gatcgagcca actacaaccc
    63121 gcaggtagcg gatgatgatg cagcagcagc aacgtatggc gttctggcgc atcggccagt
    63181 ttacgttcca gccactcaag ctgaaactcg ctcagctcac cgtgcggcac gccaaacacc
    63241 tggctatcca gcaacaggat ttgccattgc tcaccaataa acacccgctt cgccggggag
    63301 atacccgcat cctgtaacgc gctgtacatc gcgggctgga aatcgtggtt gcccggcagc
    63361 cagacgcagg gcgcacgaaa acttgcgatg ccttcagcga aatgctgata ggccgcagag
    63421 gattgatcct gcgctaaatc acctgtcgcg acaatcaggt cgaattcgtg ctggtgtgga
    63481 cgaatcgcct ccagcaccgc ctggtagctt tcccaggtgt ttacccctaa cagggcttcg
    63541 tgcttttgtg caaacaggtg agtatcggta atttgtaaaa tccttcctct ggcctcacca
    63601 gccagaggaa gggttaacag gctttccaaa tggtgtcctt aggtttcacg acgctagtaa
    63661 accggaatcg ccatcgctcc atgtgctaaa cagtatcgca accagtccgc taaaaactgg
    63721 ttaatttgat gcttttcgtc gcgttgatgc aactttttat taggataatc ataccgcgct
    63781 ttgaagcgaa aaatctgctg gcttgaacac acttcagcca ccatcgcgtc atgatacaga
    63841 cgcaccgtca ttgacggaag gctccagtaa ctgatcgcgg gcgcagtctg ttctattgtc
    63901 accagggtag tgtatcgggt cgattccaca atcgtcagcc gatattgtgc gtttgccacc
    63961 tgatagctta cagtttcgcc gggtgcgtca ttgcgcggta acaaacggcg caattgtgaa
    64021 aagttcatct cgcacaggcg catcatttca ggaaagtcag gtgtgtaacg cttcatttat
    64081 gcccactcat tttttaacgc ttgatgatgc agctgcagcc attgcaaagc gatgaccgac
    64141 gctgcgttgt cgattttccc ctcttctacc cactggtatg cctgttcccg gcttaccaca
    64201 tgaacgcgaa tatcttcgtt ttcatcagcc agaccatgaa taccgcttgc ggtcgtggcg
    64261 tccacttcgc ccaccataat tgacgaacgc tcactggtgc cccccgggct tgccaggaaa
    64321 cttaacaccg gtttggtccg tttgactatc agtcccgcct cttcaatcgc ttcgcgacgg
    64381 gcaacatctt ccacactttc accctcttca atcatcccgg caaccatctc cagtagccag
    64441 ggggtttcgc tggtgtcgta cgcggcaatc cgaatctgct caatcagcac aacttcatca
    64501 cgcactgggt caaagggtag caagactgcg gcgtgaccgc gctcaaaaat ttcccgccgt
    64561 acctcatgac tcatttgccc gttgaataga cgatgacgaa atctataaag atctaatgaa
    64621 aaaaagccgc gataaagtgt ttctcgtgca ataatttcta catcgttttt gccaaatgta
    64681 acgggcaggt tgtctggctt aagcattgtt aatgtcctag cactaatagt gaattaaatg
    64741 tgaatttcag cgacgtttga ctgccgtttg agcagtcata tgttaaattg aggcacatta
    64801 acgccctatg gcacgtaacg ccaacctttt gcggtagcgg cttctgctag aatccgcaat
    64861 aattttacag tttgatcgcg ctaaatactg cttcaccaca aggaatgcaa atgaagaaat
    64921 tgctccccat tcttatcggc ctgagccttt ctgggttcag ttcgttgagc caggccgaga
    64981 acctgatgca agtttatcag caagcacgcc ttagtaaccc ggaattgcgt aagtctgccg
    65041 ccgatcgtga tgctgccttt gaaaaaatta atgaagcgcg cagtccatta ctgccacagc
    65101 taggtttagg tgcagattac acctatagca acggctaccg cgacgcgaac ggcatcaact
    65161 cgaacgcgac cagtgcgtcc ctgcagttaa ctcaatccat ttttgatatg tcgaaatggc
    65221 gtgcgttaac gctgcaggaa aaagcagcag ggattcagga cgtcacatat cagaccgatc
    65281 agcaaacctt gatcctcaac accgcgaccg cttatttcaa cgtgttgaat gctattgacg
    65341 ttctttccta tacacaggca caaaaagaag cgatctaccg tcaattagat caaaccaccc
    65401 aacgttttaa cgtgggcctg gtagcgatca ccgacgtgca gaacgcccgc gcgcagtacg
    65461 ataccgtgct ggcgaacgaa gtgaccgcac gtaataacct tgataacgcg gtagagcagc
    65521 tgcgccagat caccggtaac tactatccgg aactggcggc gctgaatgtc gaaaacttta
    65581 aaaccgacaa accacagccg gttaacgcgc tgctgaaaga agccgaaaaa cgcaacctgt
    65641 cgctgttaca ggcacgcttg agccaggacc tggagcgcga gcaaattcgc caggcgcagg
    65701 atggtcactt accgactctg gatttaacgg cttctaccgg gatttctgac acctcttata
    65761 gcggttcgaa aacccgtggt gccgctggta cccagtatga cgatagcaat atgggccaga
    65821 acaaagttgg cctgagcttc tcgctgccga tttatcaggg cggaatggtt aactcgcagg
    65881 tgaaacaggc acagtacaac tttgttggtg ccagtgagca actggaaagc gcacatcgta
    65941 gcgtcgtgca aaccgtacgt tcctccttca acaacattaa tgcttctatc agtagtatta
    66001 acgcctacaa acaagccgta gtttccgcgc aaagctcatt agacgcgatg gaagcgggct
    66061 actcggtcgg tacgcgtacc attgttgatg tgttggatgc gaccaccacg ctgtacaacg
    66121 ccaagcaaga gctggcgaat gcgcgttata actacctgat taatcagctg aatattaagt
    66181 cagccctggg tacgttgaac gagcaggatt tgctggcact gaacaatgcg ctgagcaaac
    66241 cggtttccac taatccggaa aacgttgccc cgcaaacgcc ggaacagaat gctattgctg
    66301 atggttatgc gcctgatagc ccggcacccg tcgttcagca aacatccgca cgcactacca
    66361 ccagtaacgg tcataaccct ttccgtaact gatgacgacg acggggcttc ggccccgtct
    66421 gaacgtaagg caacgtaaag atacgggtta tctgccgcat tcttccccct tctcgcttca
    66481 atttcgacca gccatcctct attctgatgg gtatttacca ctggtcccgg aagacaaaaa
    66541 tgaaacggac aaaatccata cgccacgcat cgttccgcaa aaactggagc gcacgccatc
    66601 tgacaccagt cgctctcgcg gttgccactg ttcttatgct ggctggctgt gaaaagagtg
    66661 atgaaacagt gtctctctat caaaatgctg acgactgttc agctgcaaac ccaggcaaaa
    66721 gcgccgaatg taccaccgcg tacaacaatg cgctgaaaga agccgaacgt actgcgccga
    66781 aatacgccac ccgtgaagac tgtgttgctg aatttggtga aggtcagtgt cagcaagcac
    66841 cagcccaggc tggcatggca ccagaaaacc aggcgcaggc tcagcaatcc agcgggagtt
    66901 tctggatgcc gctgatggcc ggttacatga tggggcgtct gatgggcggc ggcgcgggat
    66961 ttgcacagca gccgctgttc tcctcgaaaa acccggccag tccggcttac ggtaaatata
    67021 ccgacgcgac gggtaaaaac tatggcgcag cccagccagg ccgcaccatg accgtaccga
    67081 agacggcaat ggcaccaaaa ccggcgacca ccactaccgt tacccgtggc ggttttggtg
    67141 aatctgttgc caaacaaagc actatgcagc gtagcgcaac cggcacctct tctcgttcaa
    67201 tgggtggctg ataccgatgg aaagagtcag tattaccgag cgcccggact ggcgtgagaa
    67261 agcccacgaa tacggtttca attttcacac catgtacggc gagccgtact ggtgtgaaga
    67321 tgcttactac aagttgaccc tcgcccaggt tgaaaagctg gaagaagtca ccgccgaact
    67381 gcaccagatg tgcctgaaag tggtggaaaa agtgatcgcc agcgatgagc tgatgaccaa
    67441 attccgcata ccaaaacaca cctggagttt tgtgcgtcaa tcatggctga ctcaccagcc
    67501 atcgctttat tcgcgtcttg atctggcgtg ggatggcact ggcgaaccta aacttctgga
    67561 aaataacgcc gatacgccaa cgtcactata cgaggcggcg ttctttcagt ggatctggct
    67621 ggaagatcag cttaacgccg gtaacttgcc ggagggcagc gaccagttta acagtctgca
    67681 agaaaaactg atcgatcgct tcgttgagct gcgtgaacag tatggcttcc agttgctgca
    67741 tctcacctgc tgtcgcgaca cggtggaaga tcgcggaacc attcagtatt tgcaggactg
    67801 cgcaaccgaa gctgaaatcg ccaccgagtt cctctacatc gacgagatag gtttaggcga
    67861 aaaaggccag ttcacggatt tacaggatca ggtgatttct aacctgttca agctttatcc
    67921 gtgggaattt atgctgcgcg aaatgttctc caccaagctg gaagatgctg gcgtacgctg
    67981 gctggaaccg gcgtggaaga gcattatctc caacaaggca cttctaccgc tactgtggga
    68041 gatgttcccg aatcacccga acctgctgcc cgcttatttt gcggaagatg atcatccgca
    68101 aatggaaaaa tatgtggtta aaccgatctt ctcccgtgaa ggcgcaaacg tgtcgatcat
    68161 tgagaacggc aaaaccattg aagcagcgga aggtccgtat ggcgaagaag ggatgattgt
    68221 tcagcaattc cacccgttac cgaaattcgg cgacagctat atgctgattg gtagctggct
    68281 ggtgaacgat caacctgccg gaattggcat tcgtgaagac cgtgcgttga tcacccagga
    68341 tatgtctcgg ttttatccac atatttttgt tgaataagcc acgataccgg atggcactcg
    68401 ccatccggta attgttagcc tatctgcacc gacagcatac ttaaactgcc catttctata
    68461 ccatcgaccg gaatggtaat tggctcctgc ccatcccaca cacctaacac atacaacaac
    68521 ggcagatagt gctctggcgt tgggttcgat aacgcgccac cttcatggtc gagataattc
    68581 accagaggat gctgctctac cggcccttgc cacgtcagat tcgctttcac atactcatta
    68641 aacgacgtcg cccacggata cggtgaacta tcaccgtgcc acttcactgt gcgcaggtta
    68701 tgcaccacgt taccgctggc gaccaacatt attccttcat cacgcagcgc cgcgagtttg
    68761 cgccccattt cgaaatgcca ggcggcaggt ttgctactgt cgatactcaa ctgcaccata
    68821 gggatatcgg cgtcaggata catcttaatc agcacgcccc acgagccgtg gtcaaagccc
    68881 caggcttctt tatccagcgt caccgggatc ggcgctaaca gctcaaccag acgctgtgcc
    68941 agcgcaggcg aacccggagc aggataatgc gtatcgtaca gcgcctgcgg gaagccacca
    69001 aagtcatgaa tcgtgggcgg cgtctccatc gcggtcactc ctgttccacg ggtaaaccag
    69061 tgagccgaaa ccaccacaat cgcttgcggg cgtggcaatg tcatccccaa cttctgccag
    69121 ctgcgggtat acaaattatc ttccagcacg ttcatcggac taccgtgacc taaaaacaat
    69181 gctggcatac gtgttgaaga catgatgata tccttaacta aaggtgtcat tttgatatcc
    69241 tcacaatacg cttgttcggc ggagtaagaa cccggataac aatgatgatg atcatcagtt
    69301 attttgacga tctgcctgaa ggtgaagatt tataaggagt tgtcgatgtc agtacctctc
    69361 attctgacca tactggcggg ggcagccacg tttattggcg cgtttctcgg cgttctcgga
    69421 caaaaaccct cgaaccgctt actggcgttt tcgctaggtt ttgcggcggg gatcatgttg
    69481 ctcatctcat taatggaaat gcttcctgcc gcactagcag ctgaaggaat gtcgccggtg
    69541 ttgggttatg gaatgtttat cttcggtctg cttggctact ttggcctgga ccgcatgttg
    69601 ccgcatgcgc atccgcagga tttaatgcaa aaatcggtgc agctgttgcc aaaatcgatc
    69661 aagcgcacag ccattctgct cactctcggc atcagtctgc ataacttccc ggaagggatt
    69721 gccacctttg tcacggcgag cagcaacctg gagctgggat ttggcatcgc actggccgtc
    69781 gcgttgcaca atatccctga aggtctggca gtggcaggcc cggtttatgc ggcaacgggg
    69841 tctaaacgca ccgcgattct gtgggcgggg atttctggac tagcagaaat tcttggtggt
    69901 gtgctggcgt ggttaatcct cggtagcatg atttccccgg tggtcatggc ggcaatcatg
    69961 gcggcggttg caggaattat ggtggcgctc tcggttgatg aattaatgcc gctcgccaaa
    70021 gagattgacc ctaataataa ccccagctat ggcgtactat gtggaatgtc agtgatggga
    70081 ttcagtttag tgctgctaca aacggcggga attggttaaa aacaacatca gaagttgtcc
    70141 agcacatgga caacttctgc cagcttcacg aaagccttat tctacctttt cggctgtctc
    70201 ttctctcgta ctgtttaagt atttgttccg cgcttcaagc agtgcgtctc tgtcctggcg
    70261 aaacgcccga caataacgtt tcaaatgcag gaaataaccc accacaacca gagcaatcaa
    70321 tacaatccag taccaggcga taaacatcct tttaccctta atatttaaca atactaaaca
    70381 tttagcgcat aaatttcaca tatccttttt cggatatatt catatggtcg cataataacg
    70441 gaacaattat atgattttga tcataatgaa aaagaatgcc tgatattaat gaaattttct
    70501 catagaaaga gaagcggaat taacatcttg catagcaggg caaaataaaa accgaagcca
    70561 gctacaactt cggtttcttg attaaggcag taattaagca gcggttttca gctggcttta
    70621 cgctcatgtg cctgacggta tgccaccagg tcttcaatag tcacgagcgc catattgtgt
    70681 ttattggcaa actcaatgca ctctggtgca cgcgccatcg tgccatcgtc attagtcagc
    70741 tcacacagca caccagccgg tttaaagcct gccagcgtca tcaaatcaat agtcgcttca
    70801 gtatgaccgc cacgcgtcag tacaccacct gcctgcgcgc gaagtgggaa aacgtggccc
    70861 gggcgattca gatcagaagg ttttgcgcca tcggcaatcg ctgcgcgaac ggtcgtaata
    70921 cgatcagcgg cagaaacacc ggtagtcaca ccttcagccg cttcaatggt cacggtaaaa
    70981 ccggtgccat aggcgctggt gttattttct accatcattg gcagatcaag ttgtttacgg
    71041 cgatcttcag tgatgcacag gcaaacaata ccgctaccgt ggcgaatggt cagcgccatc
    71101 tgctcaacgg tcatggtttc tgccgggaag atcatatcgc cttcgttttc acggtcttca
    71161 tcatcaagca ccattacacc gcgtccttca cgcagcgcag ccagtgcatt ttcaacacgt
    71221 tcgaaaggcg taccaaaaga ggaaagtagc gtctgattca tggtaaaaaa aacctcacta
    71281 aaattatggt taccagaatc agggcagtct taggagtggc ggcatatagc caaaataacg
    71341 tgagcgggtc catgcccgac agaatcgtta ctctctccca tccggactct aaccgtcggc
    71401 cccggaatta caccggatct gctgtccttt gagttcgcac ccaaagcgct cgcgggcttt
    71461 caactgagtt gatttaccgc cggtggggaa tttcgccccg ccctgagaat aagcggattc
    71521 actataacgc taatgattag cggcagcaac gcatagcttc acataattct ggtttatgac
    71581 ttacccttat cgcactacaa tggtactcaa cacctatcag tacagggaaa ctacaatgat
    71641 tgacccgaaa aaaattgagc aaatcgctcg ccaggttcac gaatcaatgc ctaaaggaat
    71701 cagggagttc ggggaagatg tggagaaaaa aatccgccaa accctacaag ctcagctgac
    71761 gcgcctcgac cttgtaagcc gtgaagagtt cgacgtccaa acgcaagtcc tgttacggac
    71821 tcgtgagaaa ctggcgctgc ttgaacaaag aatcagcgaa ctggaaaatc gcagtactga
    71881 gatcaaaaag cagccagatc cagagact
//
LOCUS       DAJTUI010000006        70636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTUI010000006 DAJTUI010000000
VERSION     DAJTUI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.006"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..720
                     /locus_tag="OO464_000445"
     CDS             <1..720
                     /locus_tag="OO464_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6225715.1"
                     /translation="DGSLLDDGRLSYSLEQSLDDDNNHNSSLNASYRSPYGTFSAGYS
                     YGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDANGASGVRIQNYPGIATDPFG
                     YAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNRGAMVAARFNANIGYRVLVT
                     VSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISSKSQSWTVRWGNQADQQCQF
                     AFSTPDSEPTTSVLQGTAQCH"
     gene            731..1822
                     /locus_tag="OO464_000446"
     CDS             731..1822
                     /locus_tag="OO464_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6225716.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1865..2725)
                     /gene="rsmI"
                     /locus_tag="OO464_000447"
     CDS             complement(1865..2725)
                     /gene="rsmI"
                     /locus_tag="OO464_000447"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6225717.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2790..4826
                     /gene="lpoA"
                     /locus_tag="OO464_000448"
     CDS             2790..4826
                     /gene="lpoA"
                     /locus_tag="OO464_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6225718.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4784..5179
                     /locus_tag="OO464_000449"
     CDS             4784..5179
                     /locus_tag="OO464_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6225719.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5199..5789
                     /gene="diaA"
                     /locus_tag="OO464_000450"
     CDS             5199..5789
                     /gene="diaA"
                     /locus_tag="OO464_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6225720.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5799..6374
                     /gene="yraP"
                     /locus_tag="OO464_000451"
     CDS             5799..6374
                     /gene="yraP"
                     /locus_tag="OO464_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6225721.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6488..7528)
                     /locus_tag="OO464_000452"
     CDS             complement(6488..7528)
                     /locus_tag="OO464_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6225722.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7601..8236)
                     /locus_tag="OO464_000453"
     CDS             complement(7601..8236)
                     /locus_tag="OO464_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6225723.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8364..8882
                     /gene="yhbO"
                     /locus_tag="OO464_000454"
     CDS             8364..8882
                     /gene="yhbO"
                     /locus_tag="OO464_000454"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6225724.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8862..9305)
                     /locus_tag="OO464_000455"
     CDS             complement(8862..9305)
                     /locus_tag="OO464_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6225725.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9356..9658
                     /gene="yhbQ"
                     /locus_tag="OO464_000456"
     CDS             9356..9658
                     /gene="yhbQ"
                     /locus_tag="OO464_000456"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6225726.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9645..10148)
                     /locus_tag="OO464_000457"
     CDS             complement(9645..10148)
                     /locus_tag="OO464_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6225727.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10142..10666)
                     /locus_tag="OO464_000458"
     CDS             complement(10142..10666)
                     /locus_tag="OO464_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6225728.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10875..11870
                     /locus_tag="OO464_000459"
     CDS             10875..11870
                     /locus_tag="OO464_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6225729.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11879..12757
                     /locus_tag="OO464_000460"
     CDS             11879..12757
                     /locus_tag="OO464_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6225730.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12838..13845
                     /locus_tag="OO464_000461"
     CDS             12838..13845
                     /locus_tag="OO464_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6225731.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13963..15207)
                     /gene="mtr"
                     /locus_tag="OO464_000462"
     CDS             complement(13963..15207)
                     /gene="mtr"
                     /locus_tag="OO464_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6225732.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15361..17250)
                     /gene="deaD"
                     /locus_tag="OO464_000463"
     CDS             complement(15361..17250)
                     /gene="deaD"
                     /locus_tag="OO464_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6225733.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17243..17323)
                     /gene="yrbN"
                     /locus_tag="OO464_000464"
     CDS             complement(17243..17323)
                     /gene="yrbN"
                     /locus_tag="OO464_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6225734.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17430..18314)
                     /gene="nlpI"
                     /locus_tag="OO464_000465"
     CDS             complement(17430..18314)
                     /gene="nlpI"
                     /locus_tag="OO464_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6225735.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18423..20558)
                     /gene="pnp"
                     /locus_tag="OO464_000466"
     CDS             complement(18423..20558)
                     /gene="pnp"
                     /locus_tag="OO464_000466"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6225736.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20805..21074)
                     /gene="rpsO"
                     /locus_tag="OO464_000467"
     CDS             complement(20805..21074)
                     /gene="rpsO"
                     /locus_tag="OO464_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6225737.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21223..22167)
                     /gene="truB"
                     /locus_tag="OO464_000468"
     CDS             complement(21223..22167)
                     /gene="truB"
                     /locus_tag="OO464_000468"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6225738.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22167..22568)
                     /gene="rbfA"
                     /locus_tag="OO464_000469"
     CDS             complement(22167..22568)
                     /gene="rbfA"
                     /locus_tag="OO464_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6225739.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22732..25380)
                     /gene="infB"
                     /locus_tag="OO464_000470"
     CDS             complement(22732..25380)
                     /gene="infB"
                     /locus_tag="OO464_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6225740.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25405..26892)
                     /gene="nusA"
                     /locus_tag="OO464_000471"
     CDS             complement(25405..26892)
                     /gene="nusA"
                     /locus_tag="OO464_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6225741.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26920..27372)
                     /gene="rimP"
                     /locus_tag="OO464_000472"
     CDS             complement(26920..27372)
                     /gene="rimP"
                     /locus_tag="OO464_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6225742.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27579..27655)
                     /locus_tag="OO464_000473"
     tRNA            complement(27579..27655)
                     /locus_tag="OO464_000473"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27619..27621),aa:Met,seq:cat)
     gene            28003..29346
                     /gene="argG"
                     /locus_tag="OO464_000474"
     CDS             28003..29346
                     /gene="argG"
                     /locus_tag="OO464_000474"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6225743.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29354..30961)
                     /locus_tag="OO464_000475"
                     /pseudo
     CDS             complement(29354..30961)
                     /locus_tag="OO464_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31439..31525)
                     /locus_tag="OO464_000476"
     tRNA            complement(31439..31525)
                     /locus_tag="OO464_000476"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31489..31491),aa:Leu,seq:gag)
     gene            complement(31540..31872)
                     /gene="secG"
                     /locus_tag="OO464_000477"
     CDS             complement(31540..31872)
                     /gene="secG"
                     /locus_tag="OO464_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6225744.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32100..33437)
                     /gene="glmM"
                     /locus_tag="OO464_000478"
     CDS             complement(32100..33437)
                     /gene="glmM"
                     /locus_tag="OO464_000478"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6225745.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33430..34278)
                     /gene="folP"
                     /locus_tag="OO464_000479"
     CDS             complement(33430..34278)
                     /gene="folP"
                     /locus_tag="OO464_000479"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6225746.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34368..36302)
                     /gene="ftsH"
                     /locus_tag="OO464_000480"
     CDS             complement(34368..36302)
                     /gene="ftsH"
                     /locus_tag="OO464_000480"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6225747.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36402..37031)
                     /gene="rlmE"
                     /locus_tag="OO464_000481"
     CDS             complement(36402..37031)
                     /gene="rlmE"
                     /locus_tag="OO464_000481"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6225748.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37157..37450
                     /gene="yhbY"
                     /locus_tag="OO464_000482"
     CDS             37157..37450
                     /gene="yhbY"
                     /locus_tag="OO464_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6225749.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37606..38082)
                     /gene="greA"
                     /locus_tag="OO464_000483"
     CDS             complement(37606..38082)
                     /gene="greA"
                     /locus_tag="OO464_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6225750.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38330..39763
                     /gene="dacB"
                     /locus_tag="OO464_000484"
     CDS             38330..39763
                     /gene="dacB"
                     /locus_tag="OO464_000484"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6225751.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(39949..41121)
                     /gene="cgtA"
                     /locus_tag="OO464_000485"
     CDS             complement(39949..41121)
                     /gene="cgtA"
                     /locus_tag="OO464_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6225752.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41137..42102)
                     /locus_tag="OO464_000486"
     CDS             complement(41137..42102)
                     /locus_tag="OO464_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6225753.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42229..42486)
                     /gene="rpmA"
                     /locus_tag="OO464_000487"
     CDS             complement(42229..42486)
                     /gene="rpmA"
                     /locus_tag="OO464_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6225754.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42507..42818)
                     /gene="rplU"
                     /locus_tag="OO464_000488"
     CDS             complement(42507..42818)
                     /gene="rplU"
                     /locus_tag="OO464_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6225755.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43076..44047
                     /gene="ispB"
                     /locus_tag="OO464_000489"
     CDS             43076..44047
                     /gene="ispB"
                     /locus_tag="OO464_000489"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6225756.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44274..44552
                     /gene="sfsB"
                     /locus_tag="OO464_000490"
     CDS             44274..44552
                     /gene="sfsB"
                     /locus_tag="OO464_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6225757.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44600..45859)
                     /gene="murA"
                     /locus_tag="OO464_000491"
     CDS             complement(44600..45859)
                     /gene="murA"
                     /locus_tag="OO464_000491"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6225758.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45914..46168)
                     /gene="ibaG"
                     /locus_tag="OO464_000492"
     CDS             complement(45914..46168)
                     /gene="ibaG"
                     /locus_tag="OO464_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6225759.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46328..46621)
                     /gene="mlaB"
                     /locus_tag="OO464_000493"
     CDS             complement(46328..46621)
                     /gene="mlaB"
                     /locus_tag="OO464_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6225760.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46621..47256)
                     /gene="mlaC"
                     /locus_tag="OO464_000494"
     CDS             complement(46621..47256)
                     /gene="mlaC"
                     /locus_tag="OO464_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6225761.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47275..47826)
                     /gene="mlaD"
                     /locus_tag="OO464_000495"
     CDS             complement(47275..47826)
                     /gene="mlaD"
                     /locus_tag="OO464_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6225762.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47831..48613)
                     /gene="mlaE"
                     /locus_tag="OO464_000496"
     CDS             complement(47831..48613)
                     /gene="mlaE"
                     /locus_tag="OO464_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6225763.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48621..49430)
                     /gene="mlaF"
                     /locus_tag="OO464_000497"
     CDS             complement(48621..49430)
                     /gene="mlaF"
                     /locus_tag="OO464_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6225764.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49640..50605
                     /locus_tag="OO464_000498"
     CDS             49640..50605
                     /locus_tag="OO464_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6225765.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50619..51605
                     /gene="kdsD"
                     /locus_tag="OO464_000499"
     CDS             50619..51605
                     /gene="kdsD"
                     /locus_tag="OO464_000499"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6225766.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51626..52192
                     /gene="kdsC"
                     /locus_tag="OO464_000500"
     CDS             51626..52192
                     /gene="kdsC"
                     /locus_tag="OO464_000500"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6225767.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52189..52764
                     /gene="lptC"
                     /locus_tag="OO464_000501"
     CDS             52189..52764
                     /gene="lptC"
                     /locus_tag="OO464_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6225768.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52733..53290
                     /gene="lptA"
                     /locus_tag="OO464_000502"
     CDS             52733..53290
                     /gene="lptA"
                     /locus_tag="OO464_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6225769.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53297..54022
                     /gene="lptB"
                     /locus_tag="OO464_000503"
     CDS             53297..54022
                     /gene="lptB"
                     /locus_tag="OO464_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6225770.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54070..55503
                     /gene="rpoN"
                     /locus_tag="OO464_000504"
     CDS             54070..55503
                     /gene="rpoN"
                     /locus_tag="OO464_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6225771.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55526..55813
                     /gene="hpf"
                     /locus_tag="OO464_000505"
     CDS             55526..55813
                     /gene="hpf"
                     /locus_tag="OO464_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6225772.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            55931..56422
                     /gene="ptsN"
                     /locus_tag="OO464_000506"
     CDS             55931..56422
                     /gene="ptsN"
                     /locus_tag="OO464_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6225773.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56468..57322
                     /gene="rapZ"
                     /locus_tag="OO464_000507"
     CDS             56468..57322
                     /gene="rapZ"
                     /locus_tag="OO464_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6225774.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57319..57591
                     /gene="npr"
                     /locus_tag="OO464_000508"
     CDS             57319..57591
                     /gene="npr"
                     /locus_tag="OO464_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6225775.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57804..58436
                     /gene="yrbL"
                     /locus_tag="OO464_000509"
     CDS             57804..58436
                     /gene="yrbL"
                     /locus_tag="OO464_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6225776.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58433..59160)
                     /gene="mtgA"
                     /locus_tag="OO464_000510"
                     /pseudo
     CDS             complement(58433..59160)
                     /gene="mtgA"
                     /locus_tag="OO464_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59157..59810)
                     /gene="elbB"
                     /locus_tag="OO464_000511"
     CDS             complement(59157..59810)
                     /gene="elbB"
                     /locus_tag="OO464_000511"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6225777.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60040..62376)
                     /gene="arcB"
                     /locus_tag="OO464_000512"
     CDS             complement(60040..62376)
                     /gene="arcB"
                     /locus_tag="OO464_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6225778.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62472..63401)
                     /locus_tag="OO464_000513"
     CDS             complement(62472..63401)
                     /locus_tag="OO464_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6225779.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64076..68536
                     /gene="gltB"
                     /locus_tag="OO464_000514"
     CDS             64076..68536
                     /gene="gltB"
                     /locus_tag="OO464_000514"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6225780.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68549..69967
                     /gene="gltD"
                     /locus_tag="OO464_000515"
     CDS             68549..69967
                     /gene="gltD"
                     /locus_tag="OO464_000515"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6225781.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70151..70630
                     /locus_tag="OO464_000516"
     CDS             70151..70630
                     /locus_tag="OO464_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR6225782.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17295 a        19154 c        17542 g        16645 t
ORIGIN      
        1 gatggctcac tgctggatga tggtcgcctg agctatagcc tggaacaaag tctggatgac
       61 gataacaacc ataacagtag cctgaacgcc agttaccgtt caccttatgg aaccttcagt
      121 gccggataca gttacggtaa tgacagcagc caatacaatt acggcgttac cggcggcgtg
      181 gttatccatc ctcatggcgt gacgctctcg caatatctgg gcaacgcttt tgcgcttatc
      241 gatgctaacg gggcttctgg cgtgaggata caaaactatc cggggattgc tactgatccc
      301 tttggctatg cagtggttcc ttatctcacg acttatcagg aaaaccgtct ctcggtagat
      361 actacgcagc tgcccgataa cgtcgatctt gaacaaacaa cacagtttgt ggtgcccaac
      421 agaggtgcaa tggtagcggc gcgtttcaac gccaatatcg gttatcgcgt acttgttaca
      481 gtcagcgatc gcaacggtaa accgttgccc tttggcgctc ttgccagcaa cgatgatacg
      541 gggcaacaaa gtatcgtcga tgagggcggc atactatatc tctctgggat atcgagtaaa
      601 tcacaaagct ggactgtacg ctggggaaat caggcagatc aacaatgtca gtttgctttt
      661 agtacaccgg attcagaacc aacaacctct gtattacaag gcacagcgca gtgccattaa
      721 ggataaaaaa atgaaaagag cgcctcttat aacaggactt ttgttgatat ccacatcctg
      781 cgcttatgcc tcctcaggag ggtgtggagc cgacagcact agcggtgcga caaattacag
      841 cagtgtggtt gatgatgtta cggtgaacca gacagataac gtgacaggac gggagtttac
      901 ctctgcaacg ctaagtagca ctaactggca atacgcctgt tcctgctctg cgggtaaggc
      961 agttaaactt gtctatatgg tcagccccgt acttaccacc actggacatc agacaggata
     1021 ttacaaactc aatgacagcc tggatattaa aaccacatta caggcaaacg acattccagg
     1081 actcacaacc gaccaggttg tctctgttaa cacccgattc acacagataa aaagcagcac
     1141 cgtatattct gctgcaaccc aaacgggtgt ttgccagggt gacacgtctc gttatggacc
     1201 cgttaatatt ggtgcaaata ccacctttac cctgtatgtc accaagccat ttctcggctc
     1261 gatgaccatt ccgaaaacgg atattgccgt cattaaaggc gcgtgggtcg atggaatggg
     1321 aagcccgtct acaggtgact tccatgattt agtcaagtta tcgattcagg gaaatctcac
     1381 cgccccacag tcgtgcaaaa ttaatcaggg cgatgttatt aaagttaatt ttggattcat
     1441 caatggtcag aagtttacca cccgcaatgc catgccagac ggttttactc cagtagactt
     1501 tgatatcact tatgactgtg gtgatacttc aaagattaaa aactcgttgc aaatgcgcat
     1561 cgacggtaca actggggtag tagaccagta caacctggtc gccaggcgaa gaagttcaga
     1621 caatgtgccc gatgtcggta ttcgtattga aaatctcggc ggcggagttg caaatattcc
     1681 ttttcagaac ggtatccttc ccgttgatcc ttccgggcat ggcaccgtca atatgcgcgc
     1741 ctggccagtt aatctggtcg gtggtgagct ggaaacagga aaatttcagg gcacagccac
     1801 cattaccgtc atggtgcggt aaaagaagac gttgcgtttt gattaacgca acgcttcggc
     1861 actgttaccc ctgctgctcc agcgcatact tatacagcgc atttttcttc acgccatgaa
     1921 tttctgcggc cagcgccgcc gcttttttca gcggcagttc tgcctgtagc agcgccagcg
     1981 tgcgcagggc atcggcgggt aagtcttctt cctgtgcttt atgaccttcg acaatcagca
     2041 ccatttcgcc tttgcgacgg ttttcatctt cctttaccca cgccagcagc tcgccaacgg
     2101 gcgcgccgtg aatggtttcc caggttttgg tcagctcacg cgccagaacc acgtagcggg
     2161 attcgcctaa taccgcaacg atatcttcca ggctatctaa cagacggtgg gtagattcat
     2221 aaaaaatcag cgtgcgcggc tccgcttcaa tggcttttag cgcatcacgg cggccttttg
     2281 atttggcagg taaaaagcct tcgtaacaga aacggtcaga tggtaaaccc gcggcgctta
     2341 acgcagtgat agcagcacac ggtccgggta gcggcaccac acgaatcccc gcttcacggc
     2401 aggtacgcac cagatggtag ccaggatcgt taattagcgg cgttccggca tcggaaacca
     2461 gcgcaatgtt ttgcccctct tgcagctttg ccagcagcgt ttcggctttt tgttgttcgt
     2521 tatggtcgtg cagcgcaaac aaccgggcat taatcccaaa atgttgcagc aataaaccgg
     2581 tgtgacgagt atcctcggcg gcaatcagat caacggcctg taatacctct aacgtacgct
     2641 gggtgatatc cgccagattg ccgattggcg tcggtacaat gtaaagctgg ccctgagaat
     2701 tatccgccga ttggtgttgt ttcattgtgt cgtccgtatt gccgatttaa tattgagcat
     2761 tgcgtaaaaa aaatatcact ggatacatta tggtaccctc aacattttct cgtttgaaag
     2821 ccgcgcgttg tctgcctgtt gttctggcag ccctgatttt cgccggttgt ggcacccata
     2881 ctcccgatca gtccactgct tatatgcagg gcacggcgca ggctgattct gccttttatc
     2941 ttcagcagat gcagcaaagc tctgatgata ccaggatcaa ctggcaatta ctcgccattc
     3001 gtgcactggt gaaagaaggt aaaaccgggc aggcggttga gttgtttaac caactaccgc
     3061 aagaactgaa cgattctcag cgtcgcgaga aaacactgct ggcggcagag attaaactgg
     3121 cgcagaaaga ttttgctggc gcgcaaaact tgctggcgaa aatcacacct gccgatttag
     3181 aacaaaacca gcaagcgcgt tactggcagg caaaaatcga tgccagccag gggcgtcctt
     3241 ccattgattt actgcgcgcg ttaattgctc aggaaccgct gcttggcgcg aaagaaaaac
     3301 agcagaatat tgatgccacc tggcaggcgc tctcctccat gactcaggaa caggcgaata
     3361 cgctggtgat caacgccgac gaaaatattc tgcaaggctg gctggatctg cagcgcgtct
     3421 ggtttgataa ccgtaacgat cccgacatga tgaaagccgg gatcgccgac tggcagaaac
     3481 gttatccgaa caatccgggc gcgaaaatgc tgccaacgca gttggttaac gtaaaagcgt
     3541 ttaaaccagc ctcgaccaac aaaatcgccc tgctgttgcc gctgaatggc caggcagcgg
     3601 tatttggtcg cactattcag caaggctttg aagccgcgaa aaatatcggc actcagccag
     3661 tggcggctca ggtagctgcc gcacctgccg cagacgtagc agaacaacct cagccgcaaa
     3721 ctgcggatag cgttgccagc ccggcacaag cctcggttag cgatctgacc ggtgatcagc
     3781 ctgcggccca gccggtgcct gtaagcgccc cggcgacaag caccgcagcg gtaagcgcac
     3841 ccgcaaatcc atccgcagag ctgaaaatct acgatacctc atcacaacca cttagccaga
     3901 tcttaagcca ggttcagcag gatggcgcga gtattgtggt tggtccgttg ctgaaaaata
     3961 acgttgaaga gttgctgaag agcaacaccc cgctgaacgt actggcactg aaccagccgg
     4021 agaatatcga aaaccgcgtc aatatttgtt acttcgcgct ttcaccggaa gacgaagcgc
     4081 gcgatgcagc gcgtcatatt cgtgaccagg gtaaacaagc gccgctggtg ctgatcccac
     4141 gcagtgcatt gggcgatcgc gtagccaatg cgtttgcgca agagtggcag aaactgggcg
     4201 gcggcaccgt tctgcaacaa aaatttggtt ccaccagcga attacgcgcg ggtgttaacg
     4261 gcggttctgg tattgcttta acgggtagcc cgattactcc cagagcgaca accgactccg
     4321 gcatgacgac caacaatcca acgctgcaaa ccacgccaac cgatgaccag ttcaccaata
     4381 atggcggtcg tgtcgatgcg gtctacattg tggcaacgcc gggtgaaatc gcttttatca
     4441 aaccgatgat cgccatgcgt aacggtagcc agagcggtgc aacgctgtac gccagttccc
     4501 gcagcgcaca aggcaccgca ggcccggatt tccgactgga gatggaaggc ttgcagtaca
     4561 gcgaaatccc gatgctggca ggcggtaatc tgccgttaat gcagcaggcg ctcagcgcgg
     4621 tgaacaacga ttattcgctg gctcgcatgt atgcgatggg cgttgatgcc tggtcgttgg
     4681 caaatcattt ctcacaaatg cgccaggttc agggttttga aatcaacggt aataccggaa
     4741 gtctgacggc taacccggat tgcgtgatta acaggaagtt atcatggcta cagtaccaac
     4801 aaggtcaggt agtccccgcc agttaaccac caaacagacc ggcgatgcgt gggaagcaca
     4861 agcgcgtcgc tggctggaag gcaaaggact gcggtttatc gccgctaacg tgaacgagcg
     4921 tggcggcgag atcgatctga taatgcgtga aggcctgacc accgtttttg tcgaggtacg
     4981 ctatcgccgc tctgcgcttt atggcggcgc ggcagccagt gtgacccgca gcaaacaaca
     5041 caaattatta cagactgccc gcttgtggct cgcgcgtcat aatgggagtt ttgatactgt
     5101 ggattgccgg ttcgatgtgg tagccttcac cgggaatgag gttgagtgga ttaaggatgc
     5161 ctttaatgac cactcataat taaggtttaa ggattagcgt gcaagaaaga attaaagctt
     5221 gcttcactga aagcattcaa actcaaattg cggcggcaga ggcgcttccg gatgccatct
     5281 cccgtgcagc catgacgctg gttcagtctc tgctcaatgg caacaaaatc ctctgttgtg
     5341 gtaatggaac ttccgctgcc aatgcacagc attttgctgc cagcatgatc aaccgtttcg
     5401 aaacggagcg gcccagctta cctgccattg cactaaatac tgataatgtt gtcttaacgg
     5461 cgattgccaa cgatcgctta catgatgaag tgtatgcaaa acaggtgcgg gcgctgggtc
     5521 atgcgggaga tgtattgtta gccatttcca cccgtggcaa cagccgcgat attgttaaag
     5581 cagttgaagc cgccgttacg cgtgatatga ccattgtggc attgaccggc tatgacggcg
     5641 gcgaacttgc aggtttgtta gggccacagg atgtggagat ccgcattcct tcgcatcgta
     5701 gtgctcgcat tcaggaaatg catatgctga cggtaaattg cctgtgcgat ctgatcgata
     5761 acacgctttt ccctcaccag gatgattaag gagaatacat gaaggcatta tcgccaatcg
     5821 cagtccttat ttccgcgctg ctgttgcaag gttgtgttgc cgctgccgta gtgggtaccg
     5881 ctgctgtggg taccaaagcc gcaactgacc cacgcagtgt cggcacccag gtggacgatg
     5941 gtaccctgga agtgcgcgtg aacagcgcat tgtcgaaaga cgaacagatt aagaaagaaa
     6001 cccgtattaa tgtcacggcc tatcagggca aagtgctgct ggttgggcag tcaccaaatg
     6061 ctgaactttc ggctcgcgcc aaacagattg ctatgggcgt agacggtgcc aacgaagtgt
     6121 ataacgagat tcgtcagggc cagccgattg gtctgggcga agcatctaac gatacgtgga
     6181 tcaccaccaa agtgcgttcg cagctcttaa ccagcgacct ggtgaaatcg tccaacgtga
     6241 aagtgaccac cgaaaacggt gaagtgttcc tgatggggct ggtgactgaa cgtgaagcga
     6301 aagcggcggc agatattgcc agccgggtga gcggcgtaaa gcgggttacc acagcgttta
     6361 cgtttattaa atagcagccc cttgtaatgc ctgatgcgac gcttgccgcg tcttatcagg
     6421 cctacaacga tgcaaaccgt aggtcggata aggcgtttac gccgcatccg acatgttttc
     6481 cctcaaatca gaacaacagc gccagcccgc cgacaatcac accagacact gccaccatcg
     6541 cccctgtcag ccataaggct ttcgccggga acgctttgcg cagcataatc agtgacggca
     6601 aactcactgc cgggagcgtc atcaacaatg ccagcgccgg agcggttccc atacctgcca
     6661 gcatcatcgt ttgtacaatc ggaatttctg ctgccgtggg aatgacaaac aagcatcctg
     6721 ctaccgccat cgccaccacc cacatcaggc tgttatcgac agcaccatcg gcatggggga
     6781 ataaccagac gcgtgcggca cccaacacca gtactgcaag gatgtaaacc gggatcgtac
     6841 tccagaaaag tgtccatagc gccctgcccc agcggctaaa aaaaccgctc tgtgcttccg
     6901 gtatgtcaat ttcgaccggt gcctgcgttt gcggtgtttc acgcacccat ttttgcacca
     6961 gcgtcgcaat cagcaacacc atcaccagcc cggccaccag acgaatcgcc gcaaaacccc
     7021 agccgaggac aaaccccata aacaccagcg tcgccgggtt taacaccgga ttgcccatcc
     7081 agaatgccag cgcaccgccc atcgatacct gttggcgacg cattcccgcc gcgaccggag
     7141 ccgcacagca ggtacacatc atgcccggca gcgaaaacag cgttcccagc agcgtgccgc
     7201 gaaagcgcga ttgcccaagc gtacgcaaca accagtcacg cgggatcagc acctgaatca
     7261 acgaaccgag gatcaccccc agcaccgccg ctttccatac tgcgaggaaa tagatcatcg
     7321 cgtaatccaa cgccgcttgc cacggattag catccgcctg cgcaaggata gatttaccga
     7381 tactgtgggt ttcggctgca gtaaacgctt tgccgtagta aggttgccat ttcacatacc
     7441 agagaccggc aatgacaacg agaaagaaaa gcgcgggttt ccaccactga atgggcgttg
     7501 ccgcctgaga tgaagactga ccagtcatag cattccccag gaataagtta tgataattga
     7561 gcgcgtgaat attacgctca ctatcaattc ttgggaataa ttattcagct cttttgcgta
     7621 attctgaaga gcttaaaatc gtcacgcctt catgctcagg ccgcatcgcc tctgccagca
     7681 tgacccgcgc cacgtctctc gcatcaatgg atttccagtt acctggtaac aaacggaaca
     7741 gcggcgcaaa aagcgtttcg ttcatccgct gtttgctacg atcgcccagt aacatcgacg
     7801 ggcgagcaat ggtcaatttc ggccagttct gggcaattaa tgcttcttcc atctcccctt
     7861 tgacgcggtt atagaaaaac ggcgagtggg cattggcacc catagcactg accaccagca
     7921 tatgctgcgc gcccagtcgc cgcccggtta atgcggtatc cactaccagc gtgtaatcgg
     7981 catgaataaa cgcctctttg ctccccgctt ctcgccgcgt ggtgccgaga caacaaaaca
     8041 caatgtcgat gggttcggtg acctgcgcca acgcgtcact cagttgcgga tcatggggat
     8101 taaacacccc gggcatatcg cccaacggac gtcgcgtcgg cgcagcaatg gcgttaactt
     8161 tcggttcatt aatcaacatc cgcagcaggt gaccgcccac cagccccgtt gcgcctgtaa
     8221 tcagtacctg actcatcttc gttcctttac agaattgtct gccttgcgct ccccggctca
     8281 tgcaccagtc ttaatagacc atgaggtaat tatccccgat tgtggaaaat tcgcctcatc
     8341 caatgcaaca acgcggagga agcatgagta agaaaattgc cgttttaatc actgatgaat
     8401 ttgaagattc agaatttact tcacccgcag acgagttccg taaagccgga cacgaagtga
     8461 ttactattga aaaacaagcg ggtaaaacgg tgaaaggcaa aaaaggagaa gccagcgtga
     8521 ccatcgataa atccatcgat gaagtgacgc cagcggagtt tgatgccctg ttgctaccgg
     8581 gtggtcattc accggattat ctgcgtggcg acaaccgttt tgtcaccttt acccgtgatt
     8641 ttgtgaatag cggcaaacct gttttcgcca tctgtcacgg tccgcagctg ctgatcagcg
     8701 ccgatgtgat tcgggggcgc aaactgaccg cagttaaacc gatcattatt gatgttaaaa
     8761 atgcgggcgt ggaattttac gatcaggaag tcgtggttga taaagatcag ctggttacca
     8821 gccggacacc ggacgatctg ccagcgttta accgcgaggc gttacgcctg ctcggtgcct
     8881 gagtcgcgta gccaaatcat ttttttacca aagcccagcg tgttgtcggt gaatttgatt
     8941 tcatcgagcc ggatttccca caccggggcc gacagcattc tggcaaccgg aaagcgacga
     9001 ttgtacgcct tgcgcgcgag gtcgctttcc tcaccttcca gcctgcggat ctcaccttta
     9061 aactgcacac cgcgaattaa cgctaccgtt ttcggctgac cgtttaccgt tccggcaacc
     9121 gcagcctgcg gcccactcat ctgcgcgtgg cgcgtttttt cttccgtcaa aatgtagaag
     9181 gcgactttct gcgcatcaaa aagataaaag gcattagcgc accagagttc cccttcctgc
     9241 tgcacacacc aggtgacaac atgttgtttt gccagccaac ggctgatggc gatgagtgtt
     9301 tccattgctg ttctctttta tactgtgggc ctgaccttaa catgcgatgc agacgatgac
     9361 accctggttt ctttacttga tccgtaccgc cgacaataag ctttataccg ggatcaccac
     9421 ggatgtcgaa cgccgctatc agcagcacca aagcggcaaa ggggcgaaag cactgcgcgg
     9481 gaaaggagaa ctgacgctgg cgttttctgc gccagtgggc gaccgttcgc tggcactacg
     9541 ggcggaatat cgcgttaagc aactgaccaa acggcaaaaa gagcgtctgg tagcggaagg
     9601 cgcagggttt gcagaactgt tgagcagcct gcaaaccccg gagattaaaa gcgattgaaa
     9661 tgctcgtgat actcaaccag accggttacg ccattcagcg cgtcatccgc taaacgatgt
     9721 accaggaagg cgctttcagt gcccggccag cggcaacgca gatcgtgatg cgccgccagt
     9781 tcaaagccga aacgactgta cagcgccgga tcgcccagcg tcaccactgc ggcatagccg
     9841 aactcattaa gcgaatcgag tccttcatag accagttggc gtgccagccc ctgtccacgg
     9901 tatttttcat caaccgccaa cggtgccatg ccgacccatt gcaggtcttc gccctgcaca
     9961 tcaaccggac taaatgccac atagccaatg acctgacctt cgtcatctgt cgccaccagc
    10021 cccagcgtca gaaagccatc ttcacgcaga tcgtgaacca gcttcgcttc cgcatcactt
    10081 tcgaatgagc gacgcagcag ggcatcaata cccggcgcat caatgggaat ttctactcga
    10141 attagcatgg ttcacctacc gatgtctgtt tggtttcagg cgcggttttc attcccgcct
    10201 caacaaaatc cgccagttgc agcagcatca tgcgtaaggc tttcggcatt tgctccagtt
    10261 caatggcgtc catcaggttt ttcacataca cccccagctc cgtatcgcct tcaatcacca
    10321 gccgacgctg gaagaagagc gtatccggat cttgtttacg cgccgcaatc atcagcagat
    10381 cgctggcgtc ggcactaaaa ctcacatcag cttgcgtgtt ctggctgacc accagtttgc
    10441 cattcaccac cgaggtaaac cattgcaggt caatatcacg cacatgaata cttaaccagc
    10501 ggccttcaag aaactccagc tcgccatcat ccagcgcctg acggaattgc cagcttaaga
    10561 cctgctcaag aacctggcgt tttagcgcaa atggcgtcag ttttaccggt acactcaaca
    10621 gagatggccc caaatgcaca atacgggaac gcagtttatc caacacgagt tttactccct
    10681 gtttcaacaa tcatcctatt ttgccatatc agaaaaataa catagcggta taaatcaaca
    10741 attccatatg aaattgctgc taccaccaat acaactttaa ctgccttaaa tcaaaaattg
    10801 tcgcagcaag gttaactaaa atcccagttc gttaacattt ttgcgttttg atagcgcaac
    10861 cttcaggaaa aattatggag ctgctctgcc ctgccggaaa tctcccggcg cttaaggcgg
    10921 ccatcgaaaa cggcgcagat gctgtttata tcgggctaaa agatgatacc aatgcccgtc
    10981 acttcgccgg ccttaacttt accgagaaaa aattgcagga agcggtgagt tttgtccatc
    11041 aacatcgccg caaacttcac atcgcgatta acacttttgc gcatccggac ggttacgccc
    11101 gttggcagcg tgccgtggat atggcggcgc agctgggtgc cgacgcgctg atcctcgccg
    11161 acctcgccat gctggagtac gccgccgagc gttacccgca tattgaacgt catgtgtcag
    11221 tgcaggcttc ggcgaccaat gaagaggcga ttaactttta tcatcgccat tttgacgttg
    11281 cccgcgtggt gctgccgcgc gtgttgtcga ttcatcaggt gaaacagctg gcacgggtta
    11341 ctcctgtccc gctggaagtg tttgctttcg gcagcctgtg tattatgtcg gaaggccgtt
    11401 gctatctgtc gtcgtatctg acgggtgagt cgcccaacac tgtgggtgcg tgttctccgg
    11461 cccgtttcgt gcgctggcaa caaacgccgc aggggctgga atcccgcctg aacgaagtgc
    11521 tgatcgaccg ttatcaggac ggcgaaaacg caggttatcc gacgctgtgt aaagggcgtt
    11581 atctggtgga cggcgagcgc tatcacgcgc tggaagaacc aaccagtctc aataccctgg
    11641 aactgctgcc ggagttaatg gctgcgaata ttgcttcggt gaaaattgaa ggccgccagc
    11701 gtagcccggc gtatgtcagc caggcggcga aagtctggcg tcaggctatc gaccgttgta
    11761 aggccgatcc gcaaaacttc gtaccgcaaa gcgcgtggat ggagacgctc gggtcgatgt
    11821 ccgaaggcac gcaaaccacc cttggcgcgt atcaccgtaa atggcagtga gaagagcaat
    11881 gaaatattcc ttagggccag tgctgtggta ctggccaaaa gagacgctgg aagattttta
    11941 tcagcaagcc gccgccagca gcgccgacgt gatttatctt ggtgaagcgg tatgcagcaa
    12001 gcgccgggca accaaagttg gcgactgtct ggagatggca aaatcgctcg ccggaagtgg
    12061 caagcagatt gtgctctcca cgctggcgct ggtgcaggca tcctctgaac tgggcgaact
    12121 gaaacgctat gttgagaacg gtgaatttct gattgaagcc agcgatctcg gcgtggtgaa
    12181 tatgtgcgct gaacgcaaac tgccgttcgt cgccgggcac gcgctgaact gctacaacgc
    12241 ggtgacgctg aaaatattgc tcaaacaggg catgatgcgc tggtgtatgc cggtggagct
    12301 ttcccgcgac tggctggtga atctgcttaa tcagtgcgat gagctgggca ttcgcaacca
    12361 gtttgaagtg gaagtcctga gctacggtca tctaccgctg gcctactccg cccgctgctt
    12421 taccgcgcgt tcggaagacc gcccgaaaga tgagtgtgaa acctgctgca ttaagtatcc
    12481 gaacgggcgc aacgtgctgt cgcaggaaaa ccaacaagtg tttgtactca atggcattca
    12541 gaccatgagc ggctacgttt acaacctcgg taacgagctg gcatccatgc agggattggt
    12601 tgatgtagtc cgcctgtcac cgcagggcac tgacactttc gcgatgctcg acgccttccg
    12661 cgctaatgaa aatggcgctg tgccactgcc gttgacggca aacagcgact gtaacggcta
    12721 ctggcggcgg ctggcaggac tggagctgca agcctaagta aatagctcac tttgttaaca
    12781 actttaacta ttctttaatg cagtattaaa gattaatcgg taacaaagtg agctgttatg
    12841 actgataaaa ccattgcgtt ttcgctactc gatctggccc ccatacccga aggctcttca
    12901 gcgcgggaag cgttctccca ctctctcgat ctcgcccgtc tggctgaaaa gcgcggctat
    12961 catcgctact ggctggcaga acaccacaat atgaccggca ttgccagtgc tgcaacatcg
    13021 gtattgattg gctatctggc ggcgaatacc accacgctgc atctggggtc tggcggcgtg
    13081 atgttgccta accacttacc gttggtcatt gccgaacagt tcggcacact caatacgctc
    13141 tatccggggc gaatcgattt ggggctgggt cgtgctccgg gtagtgacca acggacaatg
    13201 atggcgctac gtcgtcatat gagcggcgat attgataatt tcccccgcga tgtcgcggag
    13261 ctggtggact ggtttgacgc ccgcgatccc aatccgcatg tgcgcccggt accaggctat
    13321 ggcgagaaaa tccccgtgtg gttgttaggc tccagccttt acagtgcaca actggcggcg
    13381 cagcttggtc tgccatttgc gtttgcctca cacttcgcgc cggatatgtt gttccaggcg
    13441 ctgcatcttt atcgcagtaa cttcaaaccg tcggctcggc tggaaaaacc atacgcgatg
    13501 gtgtgcatca atattatcgc cgccgacagc aaccgcgacg ctgaatttct gtttacctca
    13561 atgcagcaag cctttgtgaa gctgcgccgt ggcgaaaccg ggcaactgcc gccgccgatt
    13621 caaaatatgg atcagttctg gtcaccgtct gagcagtatg acgtgcagca ggcgctgagt
    13681 atgtcgttgg taggcgataa agcgaaagtg cgtcatggtt tgcagtcgat cctgcgcgaa
    13741 accgacgctg atgagattat ggttaacggg cagattttcg accaccaggc gcgcctgcat
    13801 tcgtttgagc tggcgatgga tgttaaggaa gagttgttgg gatagtgtgt cttaacgcgg
    13861 gaagccttat ccgagctggc aacgctgtcc tacgtaggcc tgataagcga agcgcatcag
    13921 gcattgtgta ggcagcagaa atgtcggata aggcaccgct gattactgat acaccggcag
    13981 taaattaaag ctcgataaaa tatgcaccag tgcgttgccg acgccaaaca ccagaatcag
    14041 cgcaatcatc ggcttgccac cccagacgcg gaatttcggg ctgccaaagc gtttacgcga
    14101 tgcgcgggcc agcagtgccg gaacaattgc cgcccagatg gtagccgcta aaccagcata
    14161 accaatggca tacaggaatc cgttcgggaa cagcagacca cccacaacag gcggggcaaa
    14221 ggtcagcaat gccgttttca agcggcccac agccgagtcg tcgaaaccaa acagatctgc
    14281 cagatagtca aacaaaccca gcgttacgcc aaggaacgaa ctcgctaccg caaagtttga
    14341 gaacacgacc agcagaaggt ccaggctacg gctgttcagc acgccgctta atgcttgtac
    14401 caacacatca atattaccgc ccttctcggc gatgccgata aactccggac gagggatgtt
    14461 gcccattgtc gccagcaacc agatggtata cagcgccagt gccatcagcg taccgtacac
    14521 caggcatttc acgatggttt tcggatcttt gccgtaatac ttcatcaggc ttggcacgtt
    14581 accgtgataa ccaaacgacg ccaggcagaa cggcagggtc atcagcaaat acggcgcata
    14641 agatgcattg ctttcggcaa cgttgaacaa tgtcgcaggc tgcacatgcc ccagcaggct
    14701 accgaaggtg aggaagaagg taatgacttt cgcccccagc acaatcgccg tcatgcggct
    14761 gacggcttta gtactcaacc acaccacaaa cgctaccagc agtgcaaaac caaaacccgc
    14821 cgcccgtgcc gggacgttta gcgacatctc tgcgaaggtg tgatgcagaa tcgaaccact
    14881 ggcagaaata taggcatagg tcaggatata gagcacaaag gcaatggaaa tgccgttgac
    14941 cacgttccag cctttgccca gcaaatcttt ggtgatggtg tcaaaactcg aaccgattcg
    15001 gtaattcagg ttagcttcca gaatcatcaa gccggaatgc agcatacaga accaggtaaa
    15061 gatcagcgct gccattgacc agaaaaacca cgccccggac atgaccactg gcagagaaaa
    15121 catccctgcg ccaataatgg tgccgccgat aatcaccacg ccgccaagca gcgacggtga
    15181 cgtttgggtg gtggttagtg ttgccatgag ggcttctctc cagtgaaaaa tagtgcgact
    15241 gcgttgttat gcattgcact gtaccagtac atgagtacaa aagacagaaa aaaagccccg
    15301 atggttaaaa tcggggctgt atatattatt ttacagattg tgttcgctgt tcagcgatga
    15361 ttacgcatca ccaccgaaac gacgacgacc ggtagagtca tcacgacgcg gtgcgcggcc
    15421 ttcacgacgt tcgccgctaa aacgacgacc atcaccacga ccaccttcac ggcgttcacc
    15481 gctgaagtta cgaccgcctt cacgacgctc gccaccgaaa ccacgaccac cgccacgacg
    15541 ctcaccgcca gtatgcggct gtgcatcgcc cagtaactgc atgttcatcg gcttgttgag
    15601 aatgcgagtg cgcgtaaagt gttgcagcac ttcacccggc atacctttcg gcagttcgat
    15661 ggtggagtga gaagcaaaca gcttgatgtt accgatgtaa cggctgctga tgtcgccttc
    15721 gttagcaatc gcgccaacga tatgacgaac ttcaacacca tcatcgcggc ccacttcaat
    15781 gcggtacagc tgcatatcgc caacatcacg acgttcacga cgcggacgat cttcacggtc
    15841 gccacgcggg ccacggtcgt tacgatcgcg cggaccacgg tcatcacggt cacggaattc
    15901 acgtttcgga cgcatcggcg catctggcgg aacaatcaga gtacgttcac cctgtgccat
    15961 tttcagcagt gccgcagcca gagtttcgag atccagctct tcaccttctg cagtcggctg
    16021 aattttgctc agcagtgcgc ggtattgatc cagatcgctg ctttccagct gctgctgtac
    16081 tttagcggcg aatttttcca gacggcgttt gcccagcagt tctgcgttcg gcagttctac
    16141 ttccggaata gtcagcttca tagtacgttc aatgttgcgc agcagacgac gctcgcggtt
    16201 ctcaacgaac agcagcgcgc ggccagcacg acccgcacga ccggtacgac cgatacggtg
    16261 aacgtaagac tcagaatcca tcgggatatc gtagttaacc accaggctga tacgctcaac
    16321 gtccaggcca cgggccgcaa cgtcggtcgc aatcaggatg tccagacgac cgtctttaag
    16381 gcgttccagt gtctgctcac gcagcgcctg gttcatgtca ccgttcagcg cggcgctatt
    16441 gtagccgtta cgctcaagag cttcagccac ttccagagtc gcgtttttgg tacgaacgaa
    16501 gataatcgcc gcatcaaaat cttccgcttc caggaaacgt accagtgctt cgtttttacg
    16561 cataccccac acagtccagt agctctggct gatgtcagga cgggtagtca cgctggactg
    16621 aatacgcact tcctgcggct ctttcataaa gcggcgggta atgcgacgaa tcgcttccgg
    16681 catggttgca gagaacagag cggtctgatg accttccggg atctgcgcca taatggtttc
    16741 aacgtcttcg atgaagccca tgcgcagcat ttcgtcagct tcatccagaa ccagaccgct
    16801 cagtttagag aggtccagag tgccacgttt caggtggtcc agcagacggc ccggagtacc
    16861 gacaacgatc tgcggcccct gacgcagggc gcgtaattgc acgtcataac gctggccgcc
    16921 gtacagggca accacattta cgccgcgcat gtgtttagag aaatccgtca ttgcttcagc
    16981 aacctgtacc gccagttcgc gggtcggtgc cagcaccaga atctgtggtg ctttcagctc
    17041 aggatcaaga ttctgcagca gaggtaaaga gaatgctgca gtttttccgc tccccgtctg
    17101 ggccataccc agaacgtcgc ggccattcag cagatgtgga atacactctg cctgaattgg
    17161 agatggtttt tcgtaaccca gatcgttaag ggcttcaagg ataggagcct tcaggcccag
    17221 atctgcaaaa gtggtttcga attcagccat gtagtacgtg tgcctcaaaa ttaatggcgg
    17281 ccagtctaca taactcatca tgaaattgat cagcaatttt cattgaaaag tgtgaaccgg
    17341 ctcaaagtag gtgtattaac gaacaacaac gccctcaccc gttaaggtga tggcaatcaa
    17401 aaaagattac gggctgatgt gtacgtcagc tattgctggt ccgattctgc caggtcatct
    17461 tggtcctggc ccaggagcga taattccaac aatgcgtatc ggtgctcaac aaagttatga
    17521 acgttgttgg caaccgccag tttgaacagt gccgtggcgc tgtccaaatc ccccagactt
    17581 aggtagtact tacctaaata gaagttggtt tcactgagat gctcagcgag cgaggtgtta
    17641 tccgttgcgt ccgccttgag cctttccatt aacgtttgtt cgctaatgtt gcccaggtag
    17701 aactcgacaa tgttccatcc ccactgttcc ttatccgatt tttcgaagtg ctgtttcaat
    17761 acttctttag cctgcttctc atcgagcttc tgctcggcga gataaagcca cagactacgg
    17821 aaaggatcat tgggatcgtc ttgataaaac gccagcagat catcttgcgc taacttgtca
    17881 cgaccgccgt aatataatgc aatcccgcga ttcaagtgcg cgtagttgta agttggatca
    17941 agctcaagta cagaatcaaa cgcttcatag gcagcatcaa aattgcctgc ctgcgttaaa
    18001 tatatgccta agtaattgaa tacttcaggc atatccggtc ggattgccag cgcttgcgaa
    18061 aaatcgttac gcgctaatgc cctcagaccg agactatcat acaacactcc gcgctcatat
    18121 aaaagctgtg cgcgttcgtc atcggttaaa gcccgactgg caaggatttg ttccatacgt
    18181 gccagaatca cttcctgctg taaagtcggt tgcaatggta ccgcgaggac ttcactttta
    18241 cgccaggaag tattactgca tcctgcaagc gtaagtgctg tcgcaacgaa acaccagcgc
    18301 aaaaaaggct tcatttccca ctcccgaaga ccacggttga atgaacgtcc tgttcccggt
    18361 tgctaacaag gcgtcctgcc cggttaaaag ccccccgccg cagcggaggg caaatggcaa
    18421 ccttactcgc cctgttcagc agccggagct tccggtgctg cagcaggttg agactgctca
    18481 gtcgcttctt taatgctcag acggatacgg ccctggcgat caacttccag aactttcacc
    18541 ggtacttcct gacccatctg caggtaatcg gtcactttct caacgcgttt gtcagcgatt
    18601 tgagagatgt gtaccagacc ttctttaccg ccgccgatgg caacaaatgc gccaaagtca
    18661 acgatacggg tcactttacc agtgtagacg cggcccactt cgatttctgc agtgatctct
    18721 tcgatacgac gaatagcatg tttcgctttc tcgccgtcgg tcgctgcaat cttcacagta
    18781 ccgtcatctt cgatttcgat ggtggtgcca gtttcttcgg tcagggcacg gataacagag
    18841 ccgcctttac cgataacgtc tttgatcttg tccgggttga tcttgatggt atggatacgt
    18901 ggtgcgaact cagagatatc gccacgcggc gcgttgatcg cctgttccat tacgcccagg
    18961 atatgcagac gcgcaccttt agcctggttc agcgcaacct gcatgatctc tttggtgata
    19021 ccttcaattt tgatatccat ctgcaatgca gagataccct cgcgggaacc cgcaactttg
    19081 aagtccatat cgcccaggtg atcttcgtcg cccaaaatgt cagacagtac aacgtagttg
    19141 tcgccttctt tcaccagacc cattgcgata cccgcaacgg cagctttgat cggcacacct
    19201 gcgtccatca gcgccagaga cgcgccgcac acggaagcca tagaagagga accgttggat
    19261 tcagtgattt cagacacaac acgtacggtg tacgggaatt tgtccatatc cggcatgact
    19321 gccagcacgc cgcgcttcgc cagacgaccg tgaccaattt cacgacgctt cggagaaccg
    19381 accatgccgg tttcgcctac ggagtacgga gggaagttgt agtggaacag gaaggtatcg
    19441 gtacgttcgc ccatcagttc atcaagaacc tgcgcgtcac gagcagtacc cagcgttgcg
    19501 gtaaccagcg cctgcgtttc accacgggtg aacagcgcag aaccgtgagt acgcggcagc
    19561 acgccagtac gcacatccag accacggatc atatcttttt cacgaccgtc gatacgcggt
    19621 tcgcctgcca gtacgcggct acgaacaacg tttttctcga tcgcgtgcag aatttcaccc
    19681 agttcgtttt cgtccagggt ttcgtcttca gcaagcagcg tcgcgatggt ttcagatttg
    19741 atgacatcaa cctgcgcata acgctcttgt ttgtcggtga tgcggtaagc atcgctcagg
    19801 cgagcttcag ccagtgcagc aacgcgcgcg ttcagcgctt cgtttaccgg ctccggctgc
    19861 cagtcccaac gtggtttacc ggcttctttc accagttcat tgatgttctg aataacaacc
    19921 tgctgttgtt catgaccgaa cactactgcg cccagcatct ggtcttcgct cagcagttca
    19981 gcttcagatt caaccatcag tacagcggct tcagtaccgg caacaaccag atccagtttg
    20041 ctctctttca actcgtcctg agtcgggttc agtacgtact ggtcattgat gtaacctacg
    20101 cgagcagcac caatcggacc attgaacgga ataccagaca gagacagtgc tgcggaagca
    20161 ccaatcatcg cgacgatatc cgggttaact tgcgggttaa cagaaaccac ggtggcgata
    20221 acctgaactt cgttgacgaa gccttccggg aacagcggcc gaatcgggcg gtcaatcaga
    20281 cgcgcgatca gggtttcgcc ttcgcttggg cggccttcac gacggaagaa gctacccggg
    20341 atacgaccag cagcgtaggt acgctcctga tagttaacgg tcagtgggaa gaagtcctga
    20401 cctggtttgg cttttttctg gccaacaacg gtaacaaata ccgcggtgtc atccatgcta
    20461 accataacag cggcagtagc ctgacgagcc atcatgccgg tttccagagt cacggtgtgt
    20521 tggccgtact ggaatttacg aacgatcgga ttaagcaatg taatatcctt tctctttctt
    20581 agacagtacc ttacggcact ggtgttaata cccgatcttc tgcgcatcct cgcgactaat
    20641 gacaacccta acccagctct atgtgggtaa agcctctcat tagccgcgcg aacctctgca
    20701 acggaagatc attcatagca acaatacatt agtttccagt gaattgctgc cgtcagcttg
    20761 aaaaaagggg ccactcaggc ccccttttct gaaactcgca agaattagcg acgcagaccc
    20821 aggcgctcga tgagctgggt gtaacgtgct acgtctttac gtttcaggta gtcgagcagt
    20881 ttacgacgct gagaaaccat gcgcagcaga ccacgacggc tgtggtgatc ttttttgtgc
    20941 tctgcaaagt ggccctgcag gtggttgatc tgtgcagtca gcagtgctac ctgaacttca
    21001 gtagaaccgg tgtcgtttgc gtcacgacca aactcagaaa cgattttagc tgttgcttca
    21061 gtacttagag acattttaaa actccaaagt atatagaatg aaaggacgcc gatctctaat
    21121 tcagcgatcc cagtgtacgt tacgcaaagt gttaaacaat ttacgcgacg ttaagcggca
    21181 gtattctact cgtagcgacc tgttatcgca agacggttaa cattacgccg ggtattcaac
    21241 caccaggcga cgaggcgcaa cgcggccttc atcgtcaatt tcgcccatac cgataaattt
    21301 gccgttctca ccttctgtaa cgcgaaccag tccttccagc ggcgcaccgg atgtacgaac
    21361 tgggttacca tttttgaagt aaacagaaga cgttaacgga agattcacca ccgggtagtc
    21421 cgaagctgga ctgtccattg gcatcagtaa tggatcaagt aactccgcag ccggaatatc
    21481 ctgctgttcg gcttgttcga ccagttcacg cagatgttcc agggtcacaa tccgttcaac
    21541 cggatattta cttaccgcca gacgacgcag gtaaataaca tgcgcgccac aaccgagttt
    21601 ttcacccagg tcatcaatga tggtgcggat ataagtgcct tttgagcagt gaatttccag
    21661 ctccagctca ttgccttcgt ggcgaataaa cagcaattca taaacggtaa tcgggcgcgc
    21721 ttcacgcggt acttcaatgc cctgacgcgc atattcgtac agttttttac cctgatattt
    21781 cagtgctgaa tacatcgaag ggatctgttc gatatcgcca cggaaagtat ccagcgccgc
    21841 cgccagttgc tctgcgctaa aggttaccgg acgttcttca acgatttgtc cgtcggcatc
    21901 agaagtatcg gtacgctgtc caagacgcgc aatgacccga tagcgtttgt cggagtccag
    21961 cagatactgg gaaaacttcg tcgcttcccc gaggcaaatt ggcaacatgc cagttgccag
    22021 cgggtccagc gcaccggtat gcccggcacg gttggcgtta taaatccgtt tcactttttg
    22081 cagcgcatca ttgctggaca taccctgcgg tttatccagc aacaaaacgc cgttaatgtc
    22141 gcgaccgcga cgacgaggac gactcattag tcctccttgc tgtcgtccgg gttaacacga
    22201 cgttcttcgt catgtttgac cacgctggtc accaggtttg acatgcgcat cccttcaacc
    22261 agagagttgt cgtagaagaa ggtcagttcc ggcacgatac gcaggcgcat cgctttcccc
    22321 agcaggctgc ggatgaaacc agaagcttct tgcaacgctt tgatgcccgc tttaaccgcg
    22381 tcttcatctt tgtcgttgag gaacgtcaca tatactttgg catacgccag gtcgcgagac
    22441 atttcgacac cggaaacggt ggtcatcatg cccaggcgag gatctttaat ttcacgctgc
    22501 aggatgagag cgatctcttt ttgcatttcc tgcgctacgc gctgcgggcg accaaattct
    22561 ttcgccataa taaattctcc tgacaaaaaa ggggctgtta gccccttttt aaaattaatt
    22621 tcaggtggaa gggctgttca cgttggcctg ataagacgcg ccagcgtcac atcaggcaat
    22681 ccatgccgga tgcggcgtaa acgccttatc ccgcatggaa ccctaaaaac cttaagcgat
    22741 ggtacgttgg atctcgatga tttcgaatac ttcgatcaca tcgccagtgc ggacgtcgtt
    22801 gtagttctta acgccgatac cacattccat accgttacgg acttcgttaa cgtcatcttt
    22861 gaagcggcgc agggactcca gctcgccttc gtagataacc acgttgtcac gcagaacgcg
    22921 gatcgggttg tgacgtttaa ccacaccttc ggtaaccata cagcctgcga tggcaccaaa
    22981 tttcggcgat ttgaacacgt cacgaacttc cgccagaccg ataatctgct gtttcagttc
    23041 cggagacagc ataccgctca tcgccgcttt cacttcgtca atcaggttat agatgacgga
    23101 gtagtaacgc agatccaggc tttccgcttc aatcacttta cgtgcagagg catcagcacg
    23161 tacgttgaag ccaaccagga tggcgttgga cgccgcagcc agggtggcgt cggtttcggt
    23221 gataccacct acgccagaac cgatgatctt cactttaact tcgtcagtag acagtttcag
    23281 caaggagtcg gagatcgctt ccacagaacc ctgtacgtct gccttcagga cgatgttcac
    23341 ttcgtgaact tcgccttcgg tcatgttggc gaacatgttc tcgagtttag atttctgctg
    23401 acgcgccagt ttaacttcgc ggaatttacc ctgacgatac agtgcaactt cacgcgcttt
    23461 cttctcgtca cgtacaacgg taacttcatc acccgcagcc ggtacgccgg acaggccgag
    23521 gatttccacc ggaatggacg gacccgcttc cagcacttcc tgacccagtt cgttacgcat
    23581 cgcacgaaca cgaccgtatt cgaagccaca cagaacgata tcgcccttgt gcagagtacc
    23641 ttcacgtacc agaacggtag caaccggacc acgaccttta tcgaggaagg attcgataac
    23701 cgcaccgctc gccatacctt tacgtaccgc tttcagctcc agaacttccg cctgcagcag
    23761 gatagcgtcc agcagttcat cgataccagt acccgctttc gcagacacat gtacgaactg
    23821 gctttcaccg ccccactctt ccggcaggat gccgtactgg gagagttcgt tcttaacgcg
    23881 atccggatcg gcttctggtt tatcgatctt gttcactgca acgaccaccg gaacctgcgc
    23941 cgctttcgcg tgctggattg cttcgatcgt ctgcggcatt acgccgtcgt cggcagcaac
    24001 aaccaggact acgatgtccg ttgcctgcgc accacgagca cgcattgaag taaacgcggc
    24061 gtgccccggg gtgtccagga aggtgatcat gccgttttca gtttcaacgt ggtatgcacc
    24121 aatgtgctgg gtaatgccgc ccgcttcgcc agaggccact ttcgttgaac gaatgtagtc
    24181 cagcagagag gttttaccgt ggtcaacgtg acccatgatg gtcacaaccg gcgcgcgcgg
    24241 ttcagccgca gcacccgtgt cacggtcgct cattaccgcc tcttccagct cgttttcacg
    24301 acgcaggata actttatggc ccatctcttc agcaaccagc tgtgcggttt cctgatcgat
    24361 aacctggttg atggttgcca ttgcgcccag tttcatcatc gctttgatga cctgagagcc
    24421 tttaaccgcc atcttgttcg ccagttcgcc aacggtgata gtttcgccga tcacaacgtc
    24481 acggttaacg gcctgagcag gcttctggaa gccttgctgc agcgaagaac ctttacgttt
    24541 tccgccttta ccgccacgta ctgctgcgcg tgcttcttca cgatcagctt ttgattcagc
    24601 gtgtttgttg cctttcttcg gacgcgctgc tttcgcgtta cgaccacggc cacggccgcc
    24661 ttcgacttca cgatcgcttt cgtcttctgc ctggcgagca tgttgagaag tagtgacgtg
    24721 ataatcgctg gaatcttcag tcggttccgc gttatcagtc catttgtttt cttccgccat
    24781 acgacgtgct tcttcagcaa cgcgacgtgc ttcttcttcg agtttacgac gcgcttcttc
    24841 ttcagcttta cgcttgagct ctgcagcttc ctgctcacgg cgggcttttt cagcctgggc
    24901 gtttttagtc atatcgtctt gttgattgct cactttgtct ttttccgcag cttcacgttt
    24961 cgcttgttca gcagcttcac gcttagcttg ttctgcggcc tcacgttcag ctttttgttg
    25021 cgcctcgcgt ttagccgatt cttctgcctc acgacgggct tgctcttccg ctgcaaggcg
    25081 ttcagcctct tgcggatcgc gtttcacaaa ggtgcgtttc ttgcggactt cgatttgtac
    25141 cgatttgctt tttccaccgg taccaggaat gttaagggtg ctgcgtgttt tacgttgcag
    25201 cgtcaatttg tccgggcctg aatttttctg attcaggtgg tcaatcaaag tctgtttctc
    25261 ttgtgcagac acagagtcgt cagcagactt ccggatacct gcatcagcaa attgctgtac
    25321 caggcgttcc acggaggtct gtcgctctgc ggccagcgtt ttaatcgtta catctgtcat
    25381 gctgttcctt cctgctacag tttattacgc ttcgtcaccg aaccagcaaa tattacgggc
    25441 agccataatc agtgctccgg ctttttcgtc ggtcaaccct tcgatatcag ccagatcatc
    25501 aatgccctgt tcggcgagat cttccagcgt acaaacgcca cgggcggcca gtttgaatgc
    25561 caaatcacga tctacccctt caaggttcag cagatcgtca gccggtttgt tatcaccgag
    25621 gctttcttcc tgggcctgtg caatggtggc cagtgcattt ttagcacgct cgcgcagtgc
    25681 ttcaacggtc ggctcatcaa ggccttcgat ttccaacagc tctttcatcg gcacataggc
    25741 cagttcttcc agcgtcgaga aaccttcttc taccagaaca gtcgcgaagt cttcgtcgat
    25801 gtcgagatat ttggtgaagg tgtcgatcgc tgcgtgcgct tccgcctgat gcttagcctg
    25861 caggtcgtca acggtcatta cgttgagttc ccaaccgctc agctgcgaag ccagacgcac
    25921 gttctgaccg ttacggccaa tcgcctgcgc caggttacca gcttcaacgg cgatatccat
    25981 ggtgtgttta tcttcatcca ccacgataga agcaacgtct gccggtgcca ttgcgttaat
    26041 cacaaactgc gccgggttat catcccacag gacgatatcg atacgctcgc cgcccagttc
    26101 agtagaaacc gcctgaacac gcgcgccacg catacctacg caagcaccta ccggatcgat
    26161 acgtttatcg ttggttttca ccgcgatttt cgcacgagaa cccgggtcgc gagccgctgc
    26221 tttaatttca atcacttctt cgccgatttc cggcacttca atacggaaca gttcgatcag
    26281 catttccggc ttggaacgag tgacgaacag ttgcgcgcca cgcgcttccg ggcgaacgga
    26341 ataaagcacg ccacgaacgc ggtcgccagg gcggaagttt tcacgcggca gcatatcttc
    26401 gcgcaggatc acggcttcag cgttgttgcc cagatccaga gagatgttgt cgcggtttac
    26461 ttttttcacc acgccggtga tgatttcacc ttcgtgttca cggaactgat caaccaccat
    26521 cgcacgttcg gcttcacgca ctttctgcac gataacctgt tttgccgtct gggtggtgat
    26581 acggtcaaag gtaacagact caatctgatc ttcaacgtaa tcgcccaagt tcaggctttc
    26641 atcttcataa cgtgcggctt caagggtgat ttccttggtc ggctgggtga cttcatcaac
    26701 aactaaccag cgacggaagg tgtcaaaatc accgcttttg cgatcgatct gtacacggac
    26761 gtcgatctct tgctcatatt ttttctttgt tgctgtcgcc agcgcgcttt ccaatgcttc
    26821 gaaaatcttc tcgcgaggta gcgccttttc attggatacg gcttcaacta cagccaaaat
    26881 ttctttgttc atcgcgggct tttcacctca tccagactat taaaagtggg gaaccaggtt
    26941 cgccttctgg atattactca gcgcgaacac ttcatcttta ccttcgacgg taactgtgat
    27001 catttcaccg tctaccgctt tgataacgcc ctgccattta cgacggtttt gtaccgccat
    27061 acggagaacc agagtcacct cttctccgac aaaacgggcg tagtgttcag ccgtgaacag
    27121 tgggcgatcg agacccggtg aggagacttc caggttataa gcaacggtga tgggatcttc
    27181 aacatccagc acagcactta cctggtggct cacatcagca caatcatcaa cattgatgcc
    27241 atcttcacta tcaatataga tgcgcagtgt ggatgtgcga ccgcgaataa attcgatgcc
    27301 taccagttca aaacccaggg cctcaactgg cgcagtaatc atctctgtta atttttgctc
    27361 taatgtggac aagcccaccc ccaagacata aaaaaagggc ctaaagccca gttattctgt
    27421 agtcagataa caaaaaaccc cgataaatcg gggctttata taactgaacc ctataaccgc
    27481 aactgcggtc tggagcactt tccagaagga ttttttcaaa tcccactacg aaggccgaag
    27541 tcttcacagt atatttgaaa aaggactcta agggaaagtg gttgcggggg ccggatttga
    27601 accgacgacc ttcgggttat gagcccgacg agctaccagg ctgctccacc ccgcgcctga
    27661 aacgtggcaa attctactcg ttttgggtaa aaaatgcaaa tactgctggg atttggtgta
    27721 ccgagacggg acgtaaaatc tgcaggcatt atagtgatcc atgccacatt ttgtcaacgt
    27781 ttattgctaa tcacgtgaat gaatatccag ttcactttca tttgttgaat acttttgcct
    27841 tcccctgctc tcccttaagc gcattatttt acaaaaaaca cactaaactc ttcctgtctc
    27901 cgataaaaga tgattaaatg aaaactcatt tattttgcat aaaaattcag tgagagcgga
    27961 aatccaggct catcatcagt taattaagca gggtgttatt ttatgacgac gattctcaag
    28021 catctcccgg taggtcaacg tattggtatc gctttttccg gcggtctgga caccagtgcc
    28081 gcactgctgt ggatgcgaca aaagggagcg gttccttatg catatactgc aaacctgggc
    28141 cagccagacg aagaggatta tgatgcgatc cctcgtcgtg ccatggaata cggcgcggag
    28201 aacgcacgtc tgatcgactg ccgcaaacaa ctggtggccg aaggtattgc cgctattcag
    28261 tgtggcgcat ttcataacac caccggcggc ctgacctatt tcaacacgac gccgctgggc
    28321 cgcgccgtga ctggtaccat gctggttgct gcgatgaaag aagatggcgt gaatatctgg
    28381 ggtgacggta gcacctataa aggaaacgat atcgaacgtt tctatcgtta tggtctgctg
    28441 accaatgctg aactgcagat ttacaaaccg tggcttgata ctgactttat tgatgaactg
    28501 ggcggccgtc atgagatgtc tgaatttatg attgcctgcg gtttcgacta caaaatgtct
    28561 gtcgaaaaag cctactccac agactccaac atgcttggtg caacgcatga agcgaaggat
    28621 ctggaatacc tcaactccag cgtcaaaatc gtcaacccga ttatgggcgt gaaattctgg
    28681 gatgagagcg tgaagatccc ggcagaagaa gtcacagtac gctttgagca aggtcatccg
    28741 gtggcgctga acggtaaaac ctttagcgac gacgtagaaa tgatgctgga agctaaccgc
    28801 atcggcggtc gtcacggcct gggcatgagc gaccagattg agaaccgtat catcgaagcg
    28861 aaaagccgtg gtatttacga agctccgggg atggcactgc tgcacattgc gtatgaacgt
    28921 ctgttgaccg gtattcacaa cgaagacacc attgagcagt atcacgcgca tggtcgtcag
    28981 ttgggccgtc tgctgtacca ggggcgttgg tttgactccc aggcgctgat gctgcgtgac
    29041 tctctgcaac gctgggttgc cagccagatc actggtgaag ttaccctgga gctgcgccgt
    29101 gggaacgatt attcaatcct gaataccgtc tcagagaacc tgacctacaa gccagagcgt
    29161 ctgacgatgg aaaaaggcga ctcggtgttc tcgccagatg atcgtattgg tcaattgacc
    29221 atgcgtaatc tggatatcac tgatacccga gagaaacttt ttggttatgc caaaactggc
    29281 ctgctttcct cctctgccac ttcaggcctg ccgcaggtgg agaatatgga aaacaaaggc
    29341 cagtaattcg acatcaaccc tgcccttcag gggtggggtt accttgctta tcctctgtaa
    29401 actcctttct gagcatcaca taatcgaaaa catcatggtt tgcatccaca acccgtgagt
    29461 ctcctttata gtgtgcaatc acttcctcaa gcgtttgcgg ctgttccggc tttgttaccc
    29521 ccatccacgc attaataagg taattattgt aagctgtcga aaagatattg ttttccgttg
    29581 tgcgatccac gccatcccca agaacggggc tataccagat aaacatcggg acatgatatg
    29641 cctgctggct agcctccctg cctccatgaa aatagacgtt cttcttcgta gggtcacgtt
    29701 ccaggccgtg gtcggcaaaa tacatgaccg aggcgcggcg atcttttaat aattcaaaaa
    29761 cctgacctag caaactatct gtgtaatgaa tggagttgtc atagcaggca tcctgatcgt
    29821 cctgcggttg aaacacggcg ctggattgcg gataggcgct acaagccggt tcatggcttc
    29881 catttaaatg aagaacaatc agtttctttt gctgcgtatt ttgctgtaat gcttgcgata
    29941 aatgcggcaa caacaattca tcaaatcctc tgacataaac tgtttccatg gcgcgcatgg
    30001 cgatactggt aactgctgta ccattctgcc gaaaagcgga ttgcgagctt agccagaaag
    30061 tctgaaatcc tgcttgatta gccatattaa taatgttgtc ggggtaatta tgaatgtcat
    30121 gactcaaaac agaatcagca gttaaagaaa ggggaaccga cagcgcagtg taaggtgcgc
    30181 cgcttattgc ttgattaaac agtttgatct gttttctttg tgcttcaact tgcggtgtcg
    30241 tagagcgtgt atatccatac aaagacatat tgtcgacacg tacagactcc cccacaatca
    30301 acacgtaggt atcaatactt gtatccctga ctgataattg aaaatacggc accgtgtttg
    30361 caattgagag taatctttga tgcgctttcg ctgctaaagc aaaatagttg agattgaaaa
    30421 acggcgtata ggtagcaaat cacgacgcta gtatatatgg actgaacgct tttttatttt
    30481 ttgcatcttt ataagcaaat tgacaagcgg aaaataaact gcccgaaatg acaatcagca
    30541 ataatattcc tgtcactttt tttgtcggca aggaaacatc atattttatt attactgcca
    30601 aaaaaagaag ggataaaaaa gcaaaagcac atagataagg gatatacatc cccagcattt
    30661 tcacaacttc atccggatca ctctgcagca cactaatcgc aaatccatca ttgaaagttg
    30721 taccaaaggt acaccatgaa tagaggctaa tactcatgtc tgccgcagtc agtaacacaa
    30781 atggtgcggc aacaagcagg cgaagcggaa gggatgatat acgtttaaca accaggatgg
    30841 tcaggacgaa gaacagcaat cgctgaacca tatgcgctcc ggaagaggcg actaagttcg
    30901 taataccaaa aacaataaca caaagataca acaaccaatg agatttaaaa tcttctagca
    30961 aatttgttga atactgtcat ccagaaatcc aagttcacaa ccaatataaa caattatcaa
    31021 caatgttcaa ttttatatca gacatgtttt tcaactctga tccatatcag accgaaacac
    31081 tgttagtttt ttctttttaa atgatgcgac cattgcgttc tcagtaatta aataacatta
    31141 tcaatgcgtc tctaaacatt acgcagaacg tagccatcag tagttatact gacaggagca
    31201 ggatggttta tcgagaagta aacatatccg tattcgtacc ggaactggag ttgacacaat
    31261 aaagtgccaa ttatgtcagc agtagggaaa agtttataac taaggcgtac cggcaccatc
    31321 gtttcaaggt accagctacg agtaaagcaa ctggacgaga tacagatacc tgacaaccat
    31381 tcatcagacc aagaccaaaa cgaaaagaga cgcttttcag cgtctctttt ctggaatttg
    31441 gtaccgagga cgggacttga acccgtaagc cctattgggc actaccacct caaggtagcg
    31501 tgtctaccaa ttccaccacc tcggcacgga tactactttt tagttcggga tatcgctggt
    31561 cggcttagcc ggagcagctg gctgagtttg ttcggttttc gccggtgcac tcagattttc
    31621 ccattcgcta cctctattgg ttttgttgct attgatgtta cccagcacca gactgatgat
    31681 gaagaataac gttgccagca gcgccgtcat gcgggtcatg aagttaccag aaccacttga
    31741 accaaacagc gtagcggaag cgcctgctcc gaaggaggct cccatatcag cgcctttacc
    31801 ttgctgcagc atgatcagac caacaaggcc aattgccaca ataaggaaaa ctactaaaag
    31861 agcttcgtac ataatcaacc tgttccttgc ggagttgccg cgtaccaatg cttcaaccaa
    31921 taaagcggga gtttttaatc tttcccactg aagcgggtgt gaatactaac caaaacgaat
    31981 gaccttcgca agggcaattt tatcgcattg tatcaactgc ggaaaaaaac agcaaaaccg
    32041 atgtgttcgc cgagaaaaaa ggcgacaatc gccgcctttt tagccagtta tctaacgctt
    32101 taaacggctt ttactgcatc ggcgatgcgg tgtgcaaatt cagtcacctg cgcttcgtct
    32161 tcgccttcca ccatcacgcg aattaacggt tcggtgccgg atttacgcag caacacgcgt
    32221 ccacggttgc ccagcgcagc ttcaacctct gcggtcacgg ctttaactga ctcatgctca
    32281 agcggatcgc cgctacctgc ggtgtaacgt acgttaacca gaatctgcgg gaacattttc
    32341 atgccgctgc aaaggtcgtg caggctcata tggttacgtg ccatcgccgc cagcacctgc
    32401 aagccagcaa cgatgccgtc accggtagtg gttttatcca gcaggatcac atgaccggaa
    32461 ttctctgcac cgatacgcca gcctttctcc tgcatttttt ccagtacgta gcggtcaccc
    32521 actttcgcgc gcgcaaatgg aattcctaac tgtttcagcg ccagttcaag ccccatgttg
    32581 ctcatcaatg tacccacagc accaccacgc agctggccct gacgaagacc ttcacgcgcg
    32641 atgatataca tgatctgatc gccatcgact ttattgcctt catggtcaac cataatcacg
    32701 cgatcgccgt cgccgtcgaa ggcaataccg agatccgctt tttcagccag cacacgagcc
    32761 tggagcgcgc gaacgtcggt agcccccact tcggcattga tgtttacacc gtttggctca
    32821 caaccgatag cgataacgtt cgcccccagt tcgcgcagca cgttcggcgc gatgtgataa
    32881 gtcgcaccgt ttgcacaatc caccacaatc ttcagttcac tgaggctaag ttcgttcggg
    32941 aacgtggctt tgcaaaactc gatatagcga cccgcggcat caacgatacg gctggcttta
    33001 cccagttcag ccgaatcaac gcagctgatc tccttttcca tttccgcttc gatggcctct
    33061 tctaccgcat ccggcagttt agtgccgtcg atagagaaga atttaatccc attatcgtag
    33121 aacgggttat gcgatgcaga tatcacaatt ccggcctctg cgcggaaggt acgcgtcaga
    33181 taagccacgg ccggtgttgg catcgggcca gtgaagagtg cggaaaggcc cgctgccgcc
    33241 agacccgctt ccagtgctga ctccagcata tagccagaaa tacgcgtgtc tttaccaata
    33301 ataatcttac gggagccgtg gcgcgccagc actttacccg cggcccaacc cagcttaagc
    33361 acaaaatcag gtgtgatcgg cgcatcccct acacgaccac gaatcccatc ggtaccgaaa
    33421 tatttacgat tactcatagc gtttgttttc ctttgcagac agagtggctt ccaccacccg
    33481 catcgcttct acggtttctt tgacgtcatg aacacgaatg atgtgcgcgc cttgcattgc
    33541 ggcaatgacc gcacaggcca gactaccgct caggcgctcg gacggcccca cgttcagcag
    33601 ctgcccaatc atcgattttc gtgacatacc caccaacagc ggcaggttga aatggtgaaa
    33661 ttcagccagg cgcgccagta atgaatagtt atgggagaga tttttaccga aaccgaatcc
    33721 ggggtcgagc aacaatttct cttttgcgat acccgcctgc tcgcaacgtg ctatttgctc
    33781 aataaagtag cgattcactt ctgcaaagac atcgtcatac ttcggagctt cctgcatggt
    33841 ttttggattt ccctgcatat gcatcagaca aaccggtaaa ccggtttctg cagccgcctc
    33901 cagagcgcca ggttcggaaa gggagcggat atcattaata atgtgagcgc caactttcgc
    33961 tgactcacgg atgacttctg gtttggatgt atcgaccgag atccagactt cgaagcgttg
    34021 agcaattgcc tcaaccacag gaataacacg ttgcaactct tcttcaacgc taacttccgc
    34081 cgcccctggg cgcgtggact cgccaccaac gtcaatgatc gtcgcgccag cattgatcat
    34141 cagattcgca tgtttcaccg catctatcag cgagttatgc gtgccaccat ccgaaaagga
    34201 atcaggcgtg acgttgagga tccccattac gtgaggatgg ctaaggtcca gtgaagtacc
    34261 ctgggcaaag agtttcatta tgttatccct ggtatgaatt gataagaaaa aaccccggag
    34321 cacgccccgg ggttttcggt acaaatacag tcatctgatg cgggaactta cttgtcgcct
    34381 aactgctctg acatggtgtt acccgggttc ggcgtacgcg gttcatcaac cggacgagga
    34441 gcctttggac taccattgtc gccagaattg ttagaagcgc ctggttcttc ccagcccgct
    34501 ggcggacgta catcgcgacg tgccatcagg tcatcaatct gcggtgcgtc gatagtctca
    34561 tatttcatga gagcatcttt catcgcatgc agaatatcca tattgtcggt cagaagctga
    34621 cgcgcacgat tatagttacg ctcaatcagt gctttcactt cctggtcgat gatacgtgca
    34681 gtttcatcgg acatatgttt cgctttcgct acgctacggc cgaggaatac ttcaccttct
    34741 tcttccgcgt acagcagtgg acccaatttc tcagagaagc cccactgagt caccatgtta
    34801 cgtgccaggt tggtcgcaac tttaatatcg ttggacgcac cggtagaaac atgttccggc
    34861 ccgtagatga tctcttctgc cagacgacca ccgtacagcg tagaaatctg gctttccagt
    34921 ttctgacggc tggcgctgat tgcgtcgccc tcaggcaaga agaaagtcac acccagcgca
    34981 cgaccgcgtg ggataatcgt cactttatgc accggatcgt gttccggcac caggcgaccg
    35041 ataatcgcat gacccgcttc gtggtaagcc gttgattctt tctgcgcttc cgtcatcacc
    35101 atggagcgac gttccgcacc catcatgatt ttgtctttcg ctttctcgaa ctcaaccatc
    35161 gacacaacgc gtttgttgcc acgagcagcg aacagtgccg cttcgttcac caggttcgcc
    35221 aggtcagcac cggagaaacc aggagtacca cgagcaatga ttgccgcgtc gatatcgggt
    35281 gccaatggta cgcgacgcat gtgaactttc aggatctgct cacgaccgcg aacatctggc
    35341 aagccgacca caacctggcg gtcgaaacgg ccaggacgca gcagggccgg gtcgagaacg
    35401 tccggacggt tagtcgcggc gataacgatg ataccttcgt taccttcgaa gccatccatc
    35461 tcaaccagca tctggttcag agtctgttca cgttcatcgt gaccaccgcc cagaccagcg
    35521 ccacgctggc ggcctacggc gtcgatttca tcgataaaga tgatgcacgg tgccgctttc
    35581 ttcgcctgtt cgaacatgtc acgaacacgg gatgcaccca caccgacgaa catttctacg
    35641 aagtcagaac cggagatagt aaagaacgga actttcgctt cgcctgcaat cgctttcgcc
    35701 agcaacgttt taccggtacc cggaggaccg accatcagga cgcctttcgg gatcttaccg
    35761 ccgagtttct ggaagcggct cggctcgcgc agatactcta ccagttcagc aacttcttct
    35821 tttgcttcgt cgcagcccgc aacgtcagca aaggtcgttt tgatctgatc ttccgtcagc
    35881 atgcgcgctt tgctcttacc aaacgacatg gcacctttgc caccgccgcc ctgcatttga
    35941 cgcatgaaga agatccagac accaatcagc aacagcatcg ggaaccagga gatgaagata
    36001 gaagccagca ggcttggttc ttcaggcggt tcaccgacaa ccttgacgtt cttggtcaac
    36061 aggttatcca gtaatttcgg atcctgaacc ggaatgtaag tggtataacg gttactatct
    36121 ttcttggtaa cgttgatttc acgtccgttg atacgcgctt cacgaacctg gtcgttattg
    36181 acctcttgta ggaaggtaga gtaatccacc ttacggccat tagactcgct gggcccaaag
    36241 ctctggaata ctgacatcag cacaacggca atgaccagcc agagtattag gtttttcgcc
    36301 atgtcactca agggattaac ctcttattac aactgtgtta aaaacagcgt caggatactc
    36361 tatatccagc atctttcaaa ctttcgtctg aaatctcccg gttagggttt acgcccggtc
    36421 gctacaatat acacttctcg cgaacgtgca cgagaagagt ccggcttacg aactttgacc
    36481 ttcgtaaaca gggagcgaat ttcccttaga tactcatcga aaccttcgcc ctggaacacc
    36541 ttcactacaa aactgccacc tggcgctaat acatcacgac acatttctag cgccagttcc
    36601 accagataca tggctcgggg gatatccacc gccggtgttc cgctcatgtt tggtgccata
    36661 tcggacatga caacctggac tttgctgtcg ccaacgcgct ccagcagtgc tttcatcacc
    36721 agttcatcac gaaaatcgcc ctgaagaaag tccacaccaa cgataggatc cataggtaaa
    36781 agatcgcaag cgatgatgcg gcctttgccg ccaatttggg tgaccacata ttgtgaccaa
    36841 ccacccggag cagcaccaag gtcgacaacc gtcattcccg gtttaaagag tttgtcactt
    36901 tgctgtattt catcaagttt aaaccaggca cgggaacgta atcccttttt ctgtgcctgt
    36961 tgaacatatt tatcgctaaa gtgttcctga agccagcggc tggagctggc agaacgcttc
    37021 ttacctgtca tttaactttc ccatggggat aactcatcgt aaccaattgc gtaaattttt
    37081 acgcgcctat ttggtgatat atgggagatg gcggtagaat gacccgtttt caatcccaac
    37141 gtaagcaaaa aatacgatga atctgagtac taaacaaaaa cagcacctga aaggtctggc
    37201 acatccgctc aagccagttg ttctgcttgg cagtaatggt ttgaccgaag gggtgctggc
    37261 cgagattgaa caagcgttag agcaccatga actcatcaag gtgaaaatcg ccaccgaaga
    37321 tcgcgaaaca aaaaccttga tcgtggaagc tatcgtgcgc gaaaccggcg cctgtaatgt
    37381 acaggtcatc ggtaaaacgc tggtgcttta tcgcccaact aaagaacgta aaatctcgct
    37441 gccacgctaa gattatccta aagttacaca catttgctgt gtaaaacgag gggttttccg
    37501 caggcaggag agcaaaatgc cacgctctgt tcgttgataa aaggccgcag agcggccttt
    37561 ttcctttctt tacaatacat caacatcttg agtattgggt aattcttaca ggtattccac
    37621 cttaattact tcaaattcta cttcgccgcc cggcgttttg atgaccacaa catcatcttc
    37681 ttctttgccg atcaggccac gagcaatagg cgagtttaca gaaatcaggt tttgtttaaa
    37741 gtcagcttcg tcatcgccaa cgatgcgata agtctgttct tcgtcagaat ccagattcag
    37801 caccgttacg gtagcaccaa aaataacgcg cccattgttg ggcattttgg tgacatcaat
    37861 cacctgcgcg tttgacagct tggcttcgat gtctttgata cggccttcgc agaagccctg
    37921 ctgttcacga gctgcgtggt attcggcgtt ttctttcagg tcgccatgct cacgcgcttc
    37981 cgcgatagca gcaatgattt caggacggcg cacagatttc agaaaatcca gctcttcgcg
    38041 taatttttca gcgccgcgta aggtcatcgg aatagcttgc atttgttata cctcttgaat
    38101 attcctgatg gggcaagtct tcacccaccc cggctgttcg gcctgcccgg cataactcct
    38161 caccgggacc agaagcaaaa aaataccgac ccgggtacaa gtcccaggtc agctacaatt
    38221 cacattttga tggtcatttt accctgaagt tcccgaaggg tcatcgttta ctttataggg
    38281 cgttgcgccg tagtatgacg gctcgattcc aggttgttag cgcgagatta tgcgattttc
    38341 cagatttatc atcggattga ccagctgtat agcgttcagt gttcaggccg cgaatgttga
    38401 tgagtacgtt actcaactcc ccgcaggtgc caaccttgcc ctgatggtgc aaaaagtcgg
    38461 tgcgtcggcc cccgctattg attaccacag tcagcagatg gcgctgcccg ccagtaccca
    38521 gaaagtgatt actgcgctgg cggcgttgat tcaactcggc cccgattttc gtttcaccac
    38581 gacgcttgaa accaaaggca atgtggaaaa cggcgtactt aagggtgact tagtggcgcg
    38641 atttggtgcc gatccgacgt taaaacgtca ggatattcgc aatatggtcg cgactttgaa
    38701 aaaatctggc gtcaaccaaa tcgatggcaa tgtgttgata gatacctcca ttttcgccag
    38761 ccacgataaa gcccccggct ggccatggaa tgacatgaca caatgcttta gcgctccgcc
    38821 tgctgccgcc atagttgacc gcaactgttt ctccgtctcg ctctacagtg ccccaaagcc
    38881 tggtgatatg gcttttatac gcgtggcatc ttattacccc gttacgatgt tcagccaggt
    38941 acgcaccctc ccccgtggtt ctgccgaagc gcaatactgc gaactggatg tggtgccaag
    39001 cgacctgaac cgctttacgc tgacgggatg cctgccacaa cgttctgagc cgctcccgtt
    39061 ggcttttgcc gtgcaggatg gagccagcta tgccggtgca attctgaaag atgagttaaa
    39121 acaggcgggt atcacctgga gcggaacact gctgcgccag actcaggtta acgaacctgg
    39181 aacggtagtt gccagtaaac agtcggcccc gctgcacgat ctgcttaaga ttatgctgaa
    39241 aaagtcggac aacatgatcg ccgatacggt tttccgcatg atcggccatg cgcgcttcaa
    39301 tgtgcctgga acatggcggg ctgggtcgga cgccgtgcgt cagatcctgc gccagcaagc
    39361 cggtgtcgat attggtaaca ccattattgc cgatggttca gggctttcgc ggcataacct
    39421 gattgccccc gccaccatga tgcaggtgct gcaatacatt gcccaacacg acaatgaact
    39481 taactttatc tccatgctgc cgcttgcggg ctatgacggc tctttgcagt accgtgcagg
    39541 tctgcatcag gcgggcgtgg atggcaaagt ctcagcgaaa accggttcgt tgcagggggt
    39601 atataacctg gcgggattca ttaccacagc gagcgggcaa cgaatggcat ttgtacaata
    39661 tctttctggc tatgcagtaa aacccgcgga tcagcgtaat cgccgtattc cgttagtgcg
    39721 ttttgaaagc cgtttgtaca aagatattta tcagaacaat tagtcaaaaa gaaaccccgg
    39781 cgcatggccg gggtttcaga ttattgacaa agtgcgcttt gtttatgccg gatgcggcgt
    39841 gaacgcctta tccggcctac aaaatcgtgc aaattcaata tattgcaatc atcttgtagg
    39901 cctgataagt gtagcgcatc aggcttattt ggcattggtc attagtgatt aacgcttgta
    39961 aatgaactca acgccttctt cgtcgtcttc gtcccagtcg tcatcccagt cttcatcatc
    40021 ctcttcagca atctcttcaa gctgctgacg gtgataatca tcccacatga attcgacttt
    40081 ctctggctgt ttcgcttctt cagcctgcac gaccgggttt tcaatgataa aggtcatcac
    40141 atcccagcag agatctttca cgcccagtcc gctcgccgca gagatcagat aatatttatc
    40201 ttcccagccc agcgcttcag cgatcgcttt cgctttctct tcggcttctg ccttatccag
    40261 cagatcgatc ttgttgaaga ctaaccaacg cggtttcgcc gccagatcct ggctgtattt
    40321 ttccagctcg ctgataataa tacgcgcgtt ttcaaccgga tcggtgccgt caatcggatc
    40381 gatatcgatg aggtgcaaca ggacgcggca acgttccagg tgcttcagga agcgaatgcc
    40441 cagacctgcg ccttccgcag cgccttcaat cagtcctgga atatcggcaa caacgaagct
    40501 cttttcgttg tccattcgta ccacacccag acttggcacc agagtggtaa acggataatc
    40561 cgccactttc ggtttagccg ccgataccgc acgaataaag gtcgatttac ccgcgtttgg
    40621 catccccaac ataccgacgt cagccagcag catcagctcc agcagcagct cacgcttatc
    40681 gcccggcgta ccgttagttt tctgccgtgg tgtacggtta actgacgatt tgaaacgggt
    40741 attgcccaga ccgtgccagc cgcccttagc aaccagcaga cgctgaccgt gtttagtcat
    40801 atcacccatg gtttcaccag taccctggtc gattacacgc gtacctaccg gcactttaat
    40861 cgtcacgtct ttaccgcgct taccggtaca atcgcggctt gcgccgttct gaccgcgctc
    40921 tgcacggaaa gatttttcaa aacgataatc gataagcgtg ttcaggttct cgtcggcttc
    40981 catccatacg tcaccaccat caccgccgtc gccgccatcc gggccgcctt tcggaatata
    41041 cttttcgcgg cggaagctca cgcaaccatt accgccatca cctgcaacga ccagaatcga
    41101 tgcttcatca acaaacttca ttttattctc cgtaaatcat tcgcctgcgc ggggttgcga
    41161 taccaccgtt gtatgcttac gtaatccgcc ccaaatacga tgaccaatgg cggaatacat
    41221 cgcgcccgca accacgacaa acgcaccgag ataacctaaa aggtttaaca tcggtctggc
    41281 gaagaaatcg ggccaggcca gtgataaaag atctgaaaaa aacagcgtaa acagtggggt
    41341 gagcgtgatg atcgcgctca cctgcgctgc ctgccagcga gccatcgctt ccgccaaggc
    41401 gccatatcct accaaggtat tcagtccgca aaaaattaaa catgcgagct gccagtggct
    41461 aagctgcgct atcactccag gctttgccag agggaagagc gcgattgtac ataaagtgta
    41521 cagtaaaaac aggatctgcg gtgaggccag ccgacgcaat aaaacctttt gcgccacgcc
    41581 ataactcacc caaaccgtcg ccgcaccgac cccaaagata actccccagg tgtaatcggt
    41641 gagctttgta aatatctcga ccagactggt gttaaaaaac atcaccagac cgctcaggag
    41701 catcaatgcc cctacaacct gagtgctgcg cattttctct ttcaggataa atacgctggc
    41761 aaccatcatg ccaactggcg agagttgccc aatcacctgc gaagcggtcg gactcaggta
    41821 ttgcaaggat gagctgaaca ggatgaagtt cccaaacagc ccggcggtcg ccactgccaa
    41881 caaaatcaac cagcgtggct tacgaaacac gcgtaatggc ggcaacctct tcttcaccgc
    41941 aagaatggca ccaaggccaa tactcgccat caagaaacgg taaaacacga ttgtcggagg
    42001 ttccatcacc tccagcacct gcttcattgc gattggcaac gccccccagc aaattgctgt
    42061 ggtaagcgcc aaaagaatgc caatgcctgc ctgctgcttc ataccgtatt ccctgcaaag
    42121 cgcatttgcg gattaacgtc gcaccatgcg catttttacc ggttatcgaa tgtaaaaagc
    42181 cccgcaacgt gttgcggggc tttcatccgt taccgggacg cgaaaaactt attcagcttc
    42241 gatgctgata aatttacggt ttttcgggcc tttaacttcg aatttcactt taccgtctgc
    42301 tttagcaaac agagtgtggt cacgaccgca acctacgtta gcgccagcgt ggaatttggt
    42361 accacgttga cgaacgatga tgctacccgc cagaacggat tcgccaccga aacgcttaac
    42421 gcccaagcgt ttagcttctg aatcgcgacc gttacgtgtg gagccgccag cctttttatg
    42481 tgccatttga aatctctcct caggtcttag gcgctgatgc cagtaatttt cacatcagtg
    42541 aaccactgac gatggccctg ctgcttacga tagtgtttac gacgacgaaa cttaacgatt
    42601 ttaactttct cgccacgacc gtgagcaaca acttcagctt tgattacgcc gccatcaacg
    42661 aaaggaacgc cgattttgac ttcttcaccg tttgcgatca tcagcacttc agcgaactca
    42721 acagtttcgc cagttgcgat gtccagcttt tccaggcgaa cggtctgacc ttcgcttact
    42781 cggtgttgtt taccaccact ttggaaaacc gcgtacatat aaaactccgc ttccgcgcac
    42841 accttttcaa tgattcagag tgcgctataa atattcacaa tagggcgcga atattacgca
    42901 aaacgcacgc ctttgacaag tgctacagtc aatacacgaa gaaaaaaaca caacttgtac
    42961 ggtaacgttt atctgtgcca ttttttcagt acaattaccc tatattccta accataaacc
    43021 ctaagttgcc tttgttcaca gtaaggtaat cggggcgaaa agcccggctt ttgcgatgaa
    43081 tttagaaaaa atcaatgagt taaccgcgca agatatggcg ggtgttaatg cggcaatcct
    43141 tgagcagctt aattccgacg tccaactgat caatcagtta ggctattaca tcgtcagcgg
    43201 cggcggtaaa cgtattcgtc cgatgattgc tgtactggct gcacgagctg ttggctatga
    43261 gggaaatgcg catgtcacca ttgctgccct gatcgagttt atccacacgg cgactctgct
    43321 acacgacgac gttgtggatg aatcagatat gcgcaggggt aaagctaccg ccaacgccgc
    43381 atttggcaat gccgccagcg tgctggtagg cgattttatt tatacccgcg cgttccagat
    43441 gatgaccagc ctcggttcac tcaaagtgct ggaagtcatg tcagaagctg taaacgtcat
    43501 cgcagaaggt gaagttctgc aactgatgaa cgttaacgat ccggacatca ctgaagaaaa
    43561 ctacatgcgc gttatctata gcaaaaccgc gcgtctgttt gaggctgccg cgcagtgttc
    43621 cgggattctg gctggctgta cgccggagga ggagaaaggc ctgcaggatt atgggcgcta
    43681 tctcggcact gctttccagt tgatcgacga tttactcgat tacaatgccg atggcgaaca
    43741 gttaggtaaa aacgtcggcg acgatctgaa cgaaggtaaa ccgacgctgc cgctgctgca
    43801 tgcgatgcat catggcacac cagaacaggc acagatgatc cgtaccgcca tcgaacaagg
    43861 taacggtcgc catcttctgg aaccggttct ggaagcaatg aacgcttgtg gatctcttga
    43921 atggacgcgt cagcgcgccg aggaagaagc agacaaagcc atcgcagcgt tacaggtgct
    43981 cccggacacc ccttggcgag aagcactcat cggcctcgcg cacatcgctg ttcaacgcga
    44041 tcgttaatcc cctcccttca tcccgcgcag tgcgcgggat gattccagta aattccataa
    44101 aaacactaat tcagcgctat aacacctgaa aaaagggcga tgaatcttta tatattctga
    44161 atattcacac tctttacagg aactttttag cgcaatagac catcaggagt atagtgatgc
    44221 ccgacagaag aagtgttctg aatgaaagcg actaacttaa ggagtgagga aagatggaaa
    44281 gtaatttcat tgactggcat cccgctgaca tcattgcagg tttgcgcaag aaaggaacgt
    44341 caatggcggc ggaatctcgc agaaatggtt tgagttcctc aacgctggca aacgcattat
    44401 cgcgcccatg gccgaaagga gagatgatta ttgcgaaagc cctgggaact gacccctggg
    44461 ttatctggcc atcacgctac catgatccgc agacccatga gtttatcgac agaacgcagt
    44521 tgatgcgtag ctacactaaa ccgaaaaaat gagtggtctg gcggtagccc cgcgaacggg
    44581 gctgccagct ctcagacgat tactcgcctt tcacacgctc aatatttgca cctaaagcgc
    44641 gcagtttgtc ttcaatgcgt tcgtagccac gatcgatgtg ataaatacga tcaaccaccg
    44701 tcgtcccttc cgcaatacag ccagccagca ccaggcttgc tgatgcacgc agatcggttg
    44761 ccataacctg tgcaccagaa agtttttcaa caccatgaca aataacggta ttgctttcga
    44821 tttcggcgtg cgcgcccata cggctcagct ctggcacatg cataaagcgg ttttcaaaga
    44881 ccgtttcggt gatgaacccg gtcccttcag ccaccaggtt caacagcgtg aactgggcct
    44941 gcatatcggt cgggaatgcc ggatgcggcg cggtacgtac gttaacagcc ttcggacgtt
    45001 tgccatgcat atccaggcta atccagtctt cgccgacttc gatgtccgct ccagcatcac
    45061 gcagtttcgc cagcacggcg tccagagtat ctggctgcgc gttacggcag ataattttgc
    45121 cacgagagat cgccgccgcc accaggaaag taccggtttc gatacgatcc ggcagcacgc
    45181 gatagacacc gccgcctaaa cgttccacac cttcgatgac gatacgatcg gtgccctgac
    45241 cgctaatttt cgcacccagc gtaatcagga agttcgcggt atcgacgatt tccggttcac
    45301 gcgctgcgtt ttcaataatc gtggtgcctt ccgccagggt tgcagcacac atgatggtca
    45361 ccgttgcgcc aacgctgact ttatccatca cgatatgtgc gcctttcaga cgaccatcga
    45421 cggaagcttt aacgtaacct tcttccagtt tgatggtcgc gcctaattgt tcgaggccag
    45481 aaatgtgtag atcaaccgga cgcgcaccga tcgtacaacc gccaggtagt gaaacttgcc
    45541 cctgaccaaa gcgcgctacc agcggcccca gcgcccagat agaagcacgc atggttttaa
    45601 ccagatcgta aggtgcgcag aatacattaa cgtcgcgggc atcaatatgc acagaaccat
    45661 tacgttctac tttcgcaccc agctggctta gcagtttcat tgatgtatcg acgtctttca
    45721 gtttcgggac gttctggatc tctaccggtt cttccgccag tagtgcggca aaaagaatag
    45781 gcagagcagc atttttagcg ccggaaattg tgacttcgcc ctggagcttc gttggcccct
    45841 gaacacgaaa tttatccatt tagtttgttc tcagttaaca attcatatcc gctaccggcg
    45901 aatcgcccat agctcaaaag ccgttcagtt tgcgatcgcg cgcccactcc gcaggggtat
    45961 acgctttgat cgacacagca tgaatgcggt tatccgcaat atattccatc agcgggccat
    46021 agaccgtctg ctgtttttta acccgactca taccgtcaaa caactcaccc acggcaataa
    46081 cctgaaagtg gctgccatcg ccggaaacgt ggacttcctg gagggagaga gcgttcatca
    46141 acacgctctg aatttcatta ttttccatgg gatcttcaat catcagttaa taaaccagcg
    46201 aaacatctta gagcaaagtt gcgctggcat aaataagcaa aaagcctcgc tgataaatca
    46261 gacaaggctc gacttgcagg caggtttgcc ggacaggcgg ttaacgccat atccggcctg
    46321 aaaaaattta acgaggcaga acatcagcag gcaaattata caatttcgcg agggtataca
    46381 ctttgtcgtt taccccctga agcgtcacgt tgttgccctg ctttttcgcc agatcgataa
    46441 gatggagcag cagtgccagt ccccccgtat ccacgcggga cacacggcta agatcgatgc
    46501 aggtaatccc cttcaccgcc tcctcacgca tttcccaaag cggtagcaga acgtcctgat
    46561 ccagctctcc ggataacgcc agcgtgtcac ccgtctgcat ccagctcagt gactcgctca
    46621 ttattttttc tcttccagag tgattttctg ttgagaaatc gatttcagtt gcgcagtcag
    46681 gccgtcgata cctttggtac gcagcagcgt tccccactcg ttttgtttgg tggtgatcat
    46741 actgacgcct tcagcaatca tgtcgtaagc ttgccagttt cccgtctggg agtttttacg
    46801 ccactggaag tccagacgca ccggcggacg gccattcggg tcaataatgg taacgcgaat
    46861 aggcacaatg gttttatcgc ccagcggctg ttctggcgca atctgatagg tttgaccgtg
    46921 atacatcgcc agcgcctgac cgtaagcctg cttcaggtac tcacggaaag cggcaaagta
    46981 ggcttcacgt tgagcagggg tcgcactctt gtaatactgg cccagcacca gcgcaccggc
    47041 gtatttcacc tgtacgtatg gcagcagttc ctgatcaaca atggtacgca gataatccgg
    47101 gttggcccga atttgcggtt gctcattctt caggcgatcg aacgttttct gcgccgcctc
    47161 gtccatcagc ttataaggat tggtctggtc tgccgcggtt gccgcactca gaggtgcaat
    47221 caccagcaaa gcgaccatca ttaaacgttt aaacatgcgt cggttctcct gaaattattt
    47281 cgttgtaccc acaggttcag tggtttcatt attacctggc gcagcagctg gcgcatcgcc
    47341 actattctta ttgtcatcgc ctttactacc gtaaaggaac tgaccaatga gatcttccag
    47401 caccatcgca gacttggtgt cctgaattgt atcgccatcc ttcaggatag cagtccccag
    47461 ttccgggtct tcaaaaccga cgtttaatgc cagatattgt tctcccagca ggccggaagt
    47521 acgaatgctc agcgaactgg tatcaggaat gtggttataa cgttgttcaa tttccagcgt
    47581 tacgcgcggc agataggttt tcgggtccag cgtaatatcc gccacccggc ccacaacaac
    47641 accaccaatg ctgaccggag agcgcgcttt caggccgcca atgttatcga acgtcgcata
    47701 aagcgtgtag gtcgattcag tacgtatgga cgtcacgttc gccgccttca ggcaaacaaa
    47761 cagcgccgcc agcagtgctg ctaataaaaa gatacccacc caaatttcat tttttttcgt
    47821 ttgcatgaac tcaattccca aacatcaatg cggtcagcac aaaatccagc cccagaacag
    47881 ccagagacga gtggacaacg gtgcgagtgg ttgcccggct aatcccggca gacgtcggga
    47941 tggcgtcgta gccgttaaac aacgaaatcc acgtcaccgt gatggcgaac accacgctct
    48001 taatcagaca gttgaccaga tccatacgcc agtcgacggc attttgcatt gccgaccaga
    48061 agaacccgct atcaatgcct ttccaactga cgccgaccag cgatccgccc cagatcccca
    48121 cggcgacgaa gataaccgtc aacagtggta atgaaataac cccagcccag aaacggggag
    48181 aaataacccg acgcagcgga tccaccgcca tcatctccat actggagagt tgctctgtag
    48241 cgcgcatcag gccgatttct gcggttaacg ccgaaccagc acgcccggca aacaacagcg
    48301 cggcaaccac cggccccagt tcacgcagta gcgataacgc caccagcata cccagactgg
    48361 tttccgcgct ataagtggtc agaaccagat aaccttgcag ccccaacacc attccgatga
    48421 acacgccaga aactacaata atcagcatcg acaggacgcc gacattatag agctggcgca
    48481 ccagcagcgg cgcatgtttg cgaaattccg gtttgccgac cagcgcattg aataacatta
    48541 acccggcccg cccgaacgtt ctcagggttt taatcccttt atgtccgagc gacgccagcg
    48601 catttaacag catgagtggc ttaactccct ggtaaaagat cagcgtgata atcgccggca
    48661 ggatagcgga acggaacagg cccgtcagct atcccgtcca gaaactgacg tacgcgcgga
    48721 tcaggattcg tctgcaacgc ctgggcactg ccatgagcga caattttttt gtccgccagg
    48781 atccaggcgt gatccgcaat acttaacact tccggcacat cgtgagaaac caccacacaa
    48841 gtcacgccca gcgcgctgtt cagctcagaa atcagcttca ccagtacgcc catggtgatg
    48901 ggatcttgcc caacaaaagg ttcatcaaac atgatgagat ccggctccag cgcaatcgca
    48961 cgcgccagcg ctgcacgccg cgccatccca ccggaaagtt cagaaggcat tagtttagcc
    49021 gctccacgca gccccacggc ctccagcttc atcatcaccg tactatgcaa caatggcgcg
    49081 ggaagttggg tatgttcgcg cagtggatag gcgacgttgt caaatacgtt catatcagtg
    49141 aacaacgccc cggactgaaa taacatgctc atccgtttac gcactgtata caggcgcgaa
    49201 cgagacatcg ccggaatatt ctcaccatcg aaaaggatct caccatgatc tggtgcgatt
    49261 tgcccgccaa tcagacggag tagcgtcgtt ttaccgatgc ccgatggccc catgatcgcc
    49321 gtgatcttcc ctcgcggcac ggtcagggaa atattatcga agatgcagcg attgccacgc
    49381 gtaaaactga catcgcgcat atcgactaaa ttcgccacag actgctccat aattcaccct
    49441 tcgtcttgcg ttgattttct aagcatggcg ctcaatttaa ccttgaaccc aacatattta
    49501 cagaatatta cccgccgtgg ttagcgaaag ctggcatttg ttttactttt tagccgcata
    49561 aagtcaaaat taagaatccg ttacggcttt ctgaaaatct tcagcggacc ggcgagtata
    49621 cctgaagaaa ggacgttaga tgcttttagc tacggcactg ttaattgttg gtttactttt
    49681 ggtcgtttac agtgccgacc gcctggtttt tgccgcgtct attctttgcc gaacctttgg
    49741 catcccgccg ctgatcatcg gcatgacggt ggtcagtatt ggtacatcgt taccagaaat
    49801 catcgtctcg cttgccgcgt ctctgcacga acaacgcgat ttagccgttg gtacagccct
    49861 cggctcaaac attatcaata tattgctgat cctcggtctg gctgcgctgg ttcgtccttt
    49921 taccgtccat tccgatgttc tgcgccgtga attaccctta atgttgttgg tcagcgtggt
    49981 ggccggttcc gtactctatg acggacaact tagtcgaagc gatggtatct ttctcctctt
    50041 tctggctgtg ctatggctgc tgttcattgt taaacttgca cgtcagggga ctgacagcct
    50101 gaccagagag cagcttgcag agctgccgcg tgacggcgga ttgcccgtcg cgtttttatg
    50161 gctcggcatt gcgcttatca tcatgccagt ggccacgcgg atggtggttg ataacgccac
    50221 ggtgctggcg aattactttg ccatcagcga gttgacgatg gctctgacgg caattgctat
    50281 cggaaccagt ctgccggaac tggcaaccgc aatagcgggg gttcgcaaag gtgaaaacga
    50341 cattgctgtc ggaaatatca ttggcgcaaa catttttaat attgtcatcg tgttgggttt
    50401 acccgcgctg ataacgccag gagagattga tccactggcg tacagtcgtg actacagcgt
    50461 gatgttgctg gtgagcatta tttttgcgtt gctgtgctgg cggcgctccc cgcaaccggg
    50521 ccgtggtgta ggggtattat taactggcgg atttatcgta tggctggcga tgttgtactg
    50581 gttatcgcca atactcgttg aataactgga aacgcattat gtcgcacgta gagttacaac
    50641 cgggttttga ctttcagcaa gcaggtaaag aagtcctggc gattgaacgt gaatgcctgg
    50701 cggagcttga tcaatacatc aatcagaatt tcacgcttgc ctgtgaaaag atgttctggt
    50761 gtaaagggaa agttgtcgtc atggggatgg ggaaatcggg gcacatcggg cgcaaaatgg
    50821 ccgcaacgtt tgccagcacc ggtacacctt catttttcgt ccatcctagt gaagccgcgc
    50881 atggtgattt aggcatggtc accccacagg atgtggtgat tgctatctct aactcaggtg
    50941 aatccagcga aatcacggcc ttaattccag tgcttaagcg tcttcacgta ccgttaatct
    51001 gcatcaccgg tcgcccggag agcagcatgg cgcgcgccgc agatgtgcat ctgtgtgtta
    51061 aagtagcgaa agaagcctgt ccgttagggc tggcaccgac cagcagcacc accgccacgc
    51121 tggttatggg cgatgccctc gctgtcgcgc tgttaaaagc acgcggcttt actgctgaag
    51181 attttgcgct ctcacaccca ggcggcgcac tgggtcgtaa acttctgctg cgcgtaaacg
    51241 atattatgca tacgggcgat gagatcccgc atgttaagaa aacggccagt ctgcgtgacg
    51301 cattgctgga agttacccgc aaaaatcttg gtatgactgt catttgcgat gacaatatga
    51361 tgattgaagg catctttacc gacggtgatt tacgccgtgt cttcgatatg ggcgtggatg
    51421 ttcgtcagtt aagtattgcc gatgtgatga cgccgggggg aatacgtgtg cgccctggca
    51481 ttctggccgt tgaggcactg aacttaatgc agtcccgcca tatcacctcc gtgatggttg
    51541 ccgatggcga ccatttactc ggtgtgttac atatgcatga tttactgcgt gcaggcgtag
    51601 tgtaaagatt caaggataaa caacaatgag caaagcaggt gcgtcgcttg cgacctgtta
    51661 cggccctgtc agcgccgacg ttatggcaaa agcagagaac attcgtctgc tgatcctcga
    51721 cgttgatggc gtactgtcag atggcctgat ttatatgggc aataacggcg aagagctgaa
    51781 agcgttcaat gttcgtgacg gttatggcat tcgttgtgcg ctcacctctg atattgaagt
    51841 cgctatcatt accgggcgaa aggctaaact ggtagaagat cgttgtgcca cattggggat
    51901 cactcacttg tatcaggggc agtcaaacaa actgatcgcc tttagcgatt tgctggaaaa
    51961 attggcgatt gccccggaaa atgtggctta tgttggcgat gatctcatcg actggccggt
    52021 aatggaaaaa gtgggtttaa gcgtcgccgt ggccgatgcg catccactgt tgatcccgcg
    52081 cgccgattac gtgacgcgca ttgctggcgg tcgtggcgca gtgcgcgaag tttgcgactt
    52141 attactcctg gcgcagggca aactggatga agccaaaggg caatcgatat gagtaaagcc
    52201 agacgttggg ttatcattgt gctatcactg gcggttctgg tgatgatcgg cattaatatg
    52261 gccgaaaaag acgataccgc ccaggtggtc gtcaacaaca atgatcccac ctataaaagc
    52321 gagcatacgg acacgctcgt ctataaccca gaaggggcac taagctatcg attgattgct
    52381 caacacgttg aatattattc cgatcaggcc gtttcgtggt ttacgcagcc ggtacttacc
    52441 acgtttgata aggataaaat cccgacatgg tccgtaaaag cagataaagc caagctgacc
    52501 aatgaccgga tgctctattt atatggacac gttgaagtca acgcacttgt gccagactct
    52561 caacttcgca gaatcacgac ggataacgcg cagatcaatc tggtgacgca ggatgttacc
    52621 tctgaagacc tcgtcacgtt atacggaaca acatttaact ccagcggtct gaaaatgcgc
    52681 ggcaacttac gcagcaagaa cgccgagctg attgaaaagg ttagaacatc ctatgaaatt
    52741 caaaacaaac aaactcagcc ttaatcttgt gcttgccagc tcacttctgg ccgccagcat
    52801 tccggcattt gccgtaaccg gagacactga tcagccgatc cacattgaat cggaccagca
    52861 atctcttgat atgcaaggca acgtggttac ctttaccggt aatgtcatcg tcacccaggg
    52921 caccatcaaa attaatgccg acaaagtggt cgttacccgt ccgggcggcg aacaaggtaa
    52981 agaagtgatt gacggatacg gtaaaccggc aacgttctac cagatgcagg acaacggtaa
    53041 acccgttgaa ggtcacgctt cccagatgca ctacgaactg gcaaaagatt ttgtcgttct
    53101 gacgggtaat gcttatctgc agcaggtcga tagcaacatt aagggcgata agatcactta
    53161 cctggtgaaa gagcagaaaa tgcaggcttt cagcgacaaa ggcaagcgcg taaccaccgt
    53221 tctggtgccg tcgcagctgc aggacaaaaa caacaaaggc cagaccccgg cacagaagaa
    53281 gggtaattaa ttcgttatgg caacattaac tgcaaagaac cttgcaaaag cctataaagg
    53341 ccgtcgcgtg gtagaagacg tcagcctgac cgtcaactcc ggggaaattg tcggtctgct
    53401 ggggccaaac ggtgccggta agaccaccac tttctacatg gttgtaggca ttgtgccgcg
    53461 cgatgcgggc aacatcatta ttgatgatga cgatatcagt ctgctgcctc tgcatgcacg
    53521 cgcgcgccgc ggtatcggct atctgccaca ggaagcctcc attttccgtc gcctcagcgt
    53581 ttacgataac ctgatggcgg tactgcaaat tcgtgacgac ttgtctgctg aacaacgtga
    53641 agaccgcgcg aacgagctga tggaagagtt tcacattgag cacctgcgtg acagcatggg
    53701 gcagtcactc tccgggggtg aacgtcgccg tgtagaaatt gcccgcgcac tggctgcgaa
    53761 tccgaaattt attctgctcg acgaaccgtt tgccggggtt gacccgatct cggttatcga
    53821 cattaaacgc atcattgaac acctgcgcga cagcggcctg ggcgtgctga tcactgacca
    53881 caacgtgcgt gaaacactgg cggtttgtga acgcgcttat atcgtcagtc aggggcattt
    53941 gatcgcccac ggcacgccta cagaaatctt acaagacgaa cacgttaagc gtgtatacct
    54001 tggggaagac ttcagactct gatagggtag aagtttgcga cgttttagca ggagagtacg
    54061 attctgaaca tgaagcaagg tttgcaactc aggcttagcc aacaactggc gatgacgcca
    54121 cagctccaac aggcaattcg tctgttgcag ttgtcgacgc tggaacttca gcaggagcta
    54181 cagcaggcgc tggagagtaa tccgctgctt gagcaaatcg acactcatga agaaatcgac
    54241 acccgcgaaa cgcaagacag tgaaacgctg gacaccgccg acgcgctcga acaaaaagag
    54301 atgccggaag agctgccgct cgatgccagt tgggacacca tttacaccgc tggtacacca
    54361 tccggcacca gcggtgacta cattgacgac gagctgccgg tctaccaggg cgaaacgacg
    54421 cagaccttgc aggattacct gatgtggcag gtcgagctga caccgttttc cgacactgac
    54481 cgcgctattg ctacctctat cgtcgatgcc gttgatgaaa ccggttatct gactgtcccg
    54541 ctggaagata ttctcgaaag tatgggcgat gaagagatcg acatcgacga ggttgaagcc
    54601 gtccttaagc ggatccaacg gtttgacccg gtcggtgtag cggcaaaaga tctgcgtgac
    54661 tgcctgctaa tccaactctc ccaattcgat aagaccacgc cgtggctgga agaggccaga
    54721 ttgatcatta gcgatcatct cgatctgtta gccaatcacg acttccgcac tttaatgcgc
    54781 gtcacgcgtc tgaaagaaga tgtgctgaaa gaagccgtca atctgatcca gtcgctcgat
    54841 ccgcgccccg ggcagtcgat ccagactggc gaacctgagt atgtcattcc agatgtgctg
    54901 gtgcgtaagc ataacggtca ctggacggta gaactcaaca gtgacagcat tccgcgtctg
    54961 caaatcaacc agcactacgc ctcgatgtgc aataacgcgc gcaacgatgg tgacagccag
    55021 tttatccgca gcaatctgca ggatgccaaa tggttgatca agagtctgga aagccgtaac
    55081 gatacgctac tgcgcgtgag tcgctgtatc gttgaacagc agcaagcctt ctttgagcaa
    55141 ggtgaagaat atatgaaacc gatggtactg gccgatatcg cccaggctgt cgaaatgcat
    55201 gaatcgacga tatctcgcgt gaccacgcaa aaatacctgc atagtccacg aggcattttt
    55261 gaactgaagt atttcttttc cagtcacgtc aataccgagg gcggcggcga agcttcctcc
    55321 acggcgattc gtgcactggt gaagaaatta atcgcggcgg aaaacccagc gaaaccgttg
    55381 agcgacagca agttaacctc tttgctgtcg gaacaaggta tcatggtggc acgccgcact
    55441 gttgcgaagt accgagagtc tttatccatt ccgccgtcaa accagcgtaa acagctcgtt
    55501 tgacccaacc gataaggaag acactatgca gctcaacatt accggaaata acgtcgagat
    55561 caccgaggca ctgcgcgaat ttgttacagc taaatttgcc aaacttgagc aatattttga
    55621 ccgaatcaac caggtctatg ttgttctgaa agtggagaaa gtcacccaca cctcagatgc
    55681 aacactgcat gtaaacggtg gtgaaattca tgccagcgcg gaaggtcagg acatgtacgc
    55741 tgccattgat ggcttaattg ataagctggc acgccagttg accaaacata aagataaact
    55801 gaaacaacac taattgtccg ggcaattagc atgtgcatgg cggcctgttg tgcggcacaa
    55861 cgggccattt gtacggttaa tgctccgagc ctgttccact gtttgagtgg gcaggttctt
    55921 aggtgaaatt atgacaaata atgatacaac tctacagctt agcagtgttc ttaacaggga
    55981 atgtacgcga agccgcgtcc actgtcagag caaaaaacgc gccctggaaa tcatcagcga
    56041 gctggcggcg aaacaactta gcctgccgcc tcaggtggtt tttgaagcta tcctgacgcg
    56101 ggaaaaaatg ggcagtaccg gtatcggcaa tggtattgcc attccgcatg gcaaacttga
    56161 agaagatact ctgcgtgccg ttggcgtttt cgttcagctc gaaacgccta tagctttcga
    56221 tgccatcgac aaccaaccgg tggacttact ttttgccctg ctggtgccgg cagaccaaac
    56281 taaaacgcac ctgcatactc tgtcgctggt ggcgaaacgt ctggcggaca aaaccatctg
    56341 ccgccgttta cgcgcagccc agagcgatga agagctgtat caaatcatta cggataccga
    56401 aggtactccg gatgaagcgt agttattcgg taatgtctct tttagacgtt gtgaggagaa
    56461 acagtacatg gtactgatga tcgtcagcgg acgttcaggt tcaggtaaat ctgtcgccct
    56521 gcgtgcgctg gaagatatgg gtttttactg cgtggataac cttcccgtag tgttgttacc
    56581 cgatctggct cgaactctgg ccgatcgcga gatttctgcc gccgtcagca ttgatgttcg
    56641 taatatgccg gagtcaccag aaatattcga acaggcgatg agtaacctgc ctgacgcttt
    56701 ctcaccgcaa ctactgttcc tggatgccga ccgtaatacc ttaattcgtc gttacagtga
    56761 cacgcgccga ctgcatccgc tttccagcaa aaacctgtcg ctggaaagtg ctatcgacaa
    56821 agaaagcgat ttgctggagc ctctgcgttc gcgagcggat ctgattgtcg atacctcaga
    56881 aatgtccgtt cacgagctgg ctgaaatgct gcgtacccgt ctgctgggta aacgtgaacg
    56941 cgaactgacc atggtctttg agtctttcgg cttcaaacac ggtatcccta tcgatgcaga
    57001 ttacgtcttt gacgtgcgct tcttgccgaa cccgcactgg gatccgaaac tgcgtccaat
    57061 gacaggtctt gataaacctg tcgccgcgtt cctcgaccgc cacacagaag tacacaattt
    57121 tatctaccag acgcgaagct atcttgagct atggttacct atgctggaaa ccaacaaccg
    57181 tagctacctg acggtcgcca ttggttgtac cggcgggaag caccgttcgg tgtatattgc
    57241 agagcaactg gcagactact tccgctcgcg cggtaaaaac gtccagtcac gccatcgtac
    57301 gctggaaaaa cgtaaaccat gaccgtcaag caaactgttg aaatcacaaa caagctgggc
    57361 atgcatgccc ggcctgcaat gaagctgttt gaattaatgc agggttttga cgctgaagtg
    57421 ctgttacgta atgacgaagg caccgaggct gaagccaaca gcgttattgc gctgctgatg
    57481 ttggattctg ccaaaggacg gcagattgaa gttgaagcga ccggtccaca ggaagaggaa
    57541 gcactggccg ccgttatcgc cctctttaat tctggttttg atgaagatta atcttcatca
    57601 ctttgacata caactccctt caagctcccc cgacaataag aaaatcacgt actgaaatcg
    57661 ttctcaatca acgtcatttg tacattttgt gcgcttttca ctttcagaag aaccttaaga
    57721 aaaccttaag aggcattgtt taggttttgt ttaagttaat cgaccatact ggagatcgtc
    57781 agaaaatatt tccaggagat ggcatgattc gcttatctga acaaagtccc ctgggcaccg
    57841 gaagacatcg gaaatgttat gcgcatccgg aagatgccca acgctgtatc aagattgtct
    57901 accatcgtgg cgatggtggc gataaagaga tccgccgtga gttaaagtac tacgcgcatc
    57961 ttggtcgccg gttaaaagac tggagtggaa taccgcgcta tcacggtacc gttgagacgg
    58021 attgcggaac cggttatgtc tacgatgtga ttgccgattt tgacggcaag ccttccatta
    58081 cgctgaccga atttgctgaa caatgccgtt acgaagaaga cattgcacaa ctgcgccagc
    58141 tactgaaaca gctgaagcgt tatttgcagg ataaccgtat cgtgacgatg tcgctgaagc
    58201 cgcagaatat cctctgtcac cgcatcagtg agtcagaggt tatcccggtc gtctgcgaca
    58261 acattggtga aagcacattg attccactgg cgacctggtc gaagtggtgt tgtctgcgta
    58321 agcaagagcg gttatggaaa cgatttatcg cccaaccggc gctggctatt gccctgcaga
    58381 aagatctgca acctcgtgag agcaaaacgc tggccctcac ttcgcgcgaa gcttaatcca
    58441 gctggtgttg ttgcataaac ggctcaccgc ctaactgata catctgccgt aaaatccacg
    58501 cctgacggct acgcacgtag cccgatggcg aggagacttt gaaacgaagt ggattaggta
    58561 atacagctgc cagtaatgca gcttccgacc gggtaagttt gctcgcgggt ttgtggaaat
    58621 aacgttgtgc cgcagcttcg acgccaaaca caccgtcgcc aaattcggcg atattcaggt
    58681 aaaccgtcag gatacgcttt ttgctccaga ctgtttctat ccccagcgtt aatccagctt
    58741 ccagcccttt tcgtacccag ctacgcccat cccataaaaa gagattttgg ccgtctgctg
    58801 agaaatcgtt gaagcaccgc gaatacggtt ttcattgcgc tcgttgtgcg ccagggcttt
    58861 ctcaatggaa gcgacatcaa agccccagtg ctcaggaaat ttctgatctt ctgcggcaat
    58921 caccgccagt cccatccacg gcgagatttg atccatactg acccagtcag aatgtgccac
    58981 gtaacgaaaa ttgccatgca gccaggcgct gacctgtcgc tcgaccatta ccgctgagaa
    59041 gggaacaggc gcaacgctaa acaacgcgat gcccccgccc cagaaaacgg cgaggacaac
    59101 cattaaccgc aaaagaaaac ggcgaacgaa actaaacacc gttaagcggc ttttactcat
    59161 tcagccagaa ccagcacgcg ggaaaccagc ttatcaatgc cgctcgccgc ttctgcaatg
    59221 ttctgcgcca gcatatatgc tggggtggtg acaatcttat tgtcttcatc aaccacgata
    59281 tcatcgacag gacacggcac atgctccgcg cccatctctt ccagcacttc tgcggtatcg
    59341 atatcagtac cgatggtcaa acgcagcggg aaatcgaaaa ttttcggcag catcgccggg
    59401 gcaatacaca taaaaccaag cggttttccg gcctgatgca tcgcttgtgc cagcgccttt
    59461 aattcacggt caacggtgca ttcgctacca agactggcaa aattgcttaa attcttcgcc
    59521 gcgccaaacc cccccggcac aatcaacgca tccagttcag cggcatcggc ctgggccaga
    59581 ggacggattt caccgcgcgt tattcgtgcc gcttcaatca gcacattgcg cgtttccgtc
    59641 atcgcttcgc cagttaaatg gttgataaca tcaacctgct gcttatccgg tgcaaagcag
    59701 accgcctgcg caccgctgcg tgaaatagct aacaacgtca acaccgcttc atgaatttca
    59761 gaaccgtcat agacgccgca tccgctcaga attacgccaa ttttcttcat tgtgatcatc
    59821 ctttcgcaat tgactgaaac acattaattt ttttaataaa aatggtacgc atcacacatt
    59881 taactgattc atgtaacaaa tcatttaagt tttgctatct taactgcgtg cggcctgaaa
    59941 aacagtgctg tgcccttgta actcatcata ataatttacg gcgcagccaa gatttccctg
    60001 gtgttggcgc agtattcgcg caccccggtc tagccggggt cattttttag tggcttttgc
    60061 cacccacgct ttcagcactt ctacgtcgtg acgccactct tctttcatct cttcaatcca
    60121 ttcaccgacg ttatcttccc aggcgggaag gtcaggagac tgaatttgct gacccagctg
    60181 ttgcagatgg cgtaacccca ctgaccccgc cgcaccttta attttatgtc cttcctcaac
    60241 aatgcctttt ttatcctgcg ccgtcagatt cgactccagc acgctgacat agcccggcat
    60301 catcttctca aacaccgcta acccgtcggt gatcagcttc ggtcctacaa gttcgagata
    60361 ctgttccagc atgggaatat cgagcaatgc ttctgattta ctgttctctt ctgtcgtcac
    60421 cgtactctcc tcatcatcct gggtatccca gaatttcttg atcatcgcgg ttagcgccgg
    60481 aaccgaaagc ggcttactca gcacatcatc cattccagca ttgaggtact cttgtttgtc
    60541 tttcagcacg ttagcggtta aggccaccag cggcggtaaa tcctcgcgcg gataacgctt
    60601 cgtcagttca cgagagatat ccagcccggt catatctggc aactgaatat ccagcaacac
    60661 caggtcgtat tcgcccggtt taaacatctc cagcgccgcc ttgccggtca tggcgacatc
    60721 aacgctgtta cctaattttt ccagcacaga acgcgcgaca atcacgttca gttcaatgtc
    60781 ttccaccagc agcacattca gcgccggtaa aggcatatcg tcttcatcaa acgcatcatc
    60841 gacctcttct gccaccgacg gtgcgtggat cgtcaacgta aaggttgaac ctttgccctg
    60901 ttcactggta accgtaatat cgccgcccat atttttcgcc agacgacgag aaacggccag
    60961 accaataccg gtgccggtgg caggtttacc gccatgactg tctttcacct ggtaatacat
    61021 ggcgaagatt ttatccagct catcctgcgg aatgccgata ccagaatctt ccacttcaaa
    61081 atgcagcata tcgccttcat cgtagcgcac gcgcacggta acctggcctt gctgggtgaa
    61141 tttgacggcg ttactgatga ggttccacag gatctgccgt aaacgcgtcc cgtcggtaat
    61201 gacctgatgc ggtaatggca gcgtaggctc caggttaaag cgcaatcctt tttgttgcgc
    61261 ctgcaatgcg gagagatttt ccagatcggc aaggaagctg gtgaaatcaa ccggctgatt
    61321 atcaagctgg accttgcgcc gttccatctt atccatgtcg ataatatcgt taaagatatt
    61381 ccccagcgtg acggccgaaa catggatagt tttgagatat ttttcctgct cggcggtgag
    61441 ttcggtatcc agcagaatgc ggctcaggcc gacgataccg ttcagcggtg tacgcaattc
    61501 gtgactgatg gtggaaataa acgtcgtttt gtcgcggctg gcgcgttcaa gcgcatcctg
    61561 ataccgctta cgctcggtaa tgtcgcgacc aaagcccatc aaaccgtgac gtttacccac
    61621 gcggtcgtag tacggcactt tacggatttc aaagcaggct ttgcgcccgt ccgggtaatc
    61681 cagccactgt tcataggtca gtgacacatt atgacggaac actttttcat cggtttcaat
    61741 gacttttgcg gcggcttccg gtgagtaaac atcagcaggt ttcaggtgaa ccagttgttt
    61801 ttcgcttttt ccggtcagca gctccatcgc gcggttacag ccggaaaact ctttatcttc
    61861 gttacgataa aaaaccaagt cgggtgaagc atcaaggaag gaacgtaaga atgaggattg
    61921 ctgctcgagc tgaatttgtg tctcttcgcg ctctttgatt tcaattttca gttggccgaa
    61981 ggtttcctgc agttctgctt ccgctttttc acgaacggca atttcctgat ttagctgggc
    62041 aatattatct tttaactgaa cgttgaggct caaatcgcgc tcgcgcatct cctccagttt
    62101 ttgcaccagc cgtgacagac gttgtcgtga ctcctccagt tgctcgacga ccaccgatag
    62161 aaagtagacc gcccacggcg taatcagcaa accaaagaag atagaacgaa taacatcaat
    62221 gctttcgacc tgaccatgca gcaccatggt taccgccatt tgtaccacaa tggcaagaac
    62281 gacgagggcc agcgccagca acattgagaa gcgcaccaga cctaacttca tcatcaggtc
    62341 aacataatac tgcgccagca gacgaatttg cttcattagg gaattccttc acgacaacct
    62401 gccccaataa tacccaattc tgacagttac gttataaatt gtgtgagaaa tgcggaattc
    62461 ccctcaacga cctactccgt tggaggtagc cacggacgtc ccagcgccga tccctgtacg
    62521 ccatgttcat tcagatagcg atccagctcg accattcccg tccatcgatt ttcgcaccac
    62581 agcggagcaa gcagcgtcgg gcgacgggcg ctggcggaaa tgcggtggta gatcacttcc
    62641 ggtggcgtat ggcgaatcat ttctccggca gtgagcgtat aatcctccag ttcaataccg
    62701 ttcaaacgcc ccgcttccca ggctttcgcc ataatgctgc ctttcacaat atgcagcgga
    62761 tgcagcttta tgccgtccac gctggtttca accactcgct ccagcgtttg caaacattca
    62821 gcctgccctt cgccaggcag gccgacaatt aagtgggaac acaccttcag cccgcgctga
    62881 cgtgccagct gggttgtacg ctgataacag gcaaaatcat ggccgcggtt gatgcgatgc
    62941 agtgttttgt cgtgggcggt ttgtagcccc agctccagcc acacttcgta accctggtcc
    63001 ttatattcgc aaagcagatc cagcaccgca tccggcacgc agtccgggcg ggtaccaaca
    63061 cacaaaccga caatattggc ctggctcacc gcctgctgat acatagaacg cagcacctga
    63121 acttccgcaa aggtgctggt atacgcctga aagtaggcca gatagcgttt agcgcggtta
    63181 actaaattcg cctggtgcgc cagttgctcg gcaatggaac gatgctgctg cgcttcatcg
    63241 gcaaacgagg caacattaca gaatgtgcag ccaccacgcc cgatggtacc gtcacggtta
    63301 gggcagctaa aaccgccgtg cagcgtcagc ttatgcacct tttgcccata acgacgggtg
    63361 agatcaccac caaacatatt gactaatttc tgtaactgca taatctgata gaccgcgcct
    63421 tgaaaagagg ccaaagcctg ccatttttag ctcaattcgg cgatgacctg gatcaatcgt
    63481 cccggcctgc ttttatcaac tgcataatca atcaaaatta ccgaaatttc atgcacaatc
    63541 acataaatca cttttgctta tcttgtgtca gattttttta tctccttatg gattttaggc
    63601 aaaaacagta gcatgaaacg tcattaccaa ttaaggcagt ataaaatgct ggttttctcg
    63661 tcagttcaag gcaggataag ggttaacacg cctttatgac agtcaggaat tgactgtttc
    63721 tctaacgact ctccttttag ccttaaagat aaaatctatt ttaatttcag ctatttaata
    63781 aagaattttg cgctaaagca catttctgta ccaataagct tgccatttga cctgtatcag
    63841 ctttcccgat aagttggaaa tccgctggaa gctttctgga tgagcagcct gctcatcata
    63901 tttatgcagt aattgagatc ccctcttcac cgtattaacc gatgcgaaaa ggacaacaag
    63961 ggggcgaatg cgaggcgcgc gtatgacacg caaaccccgt cgccacgctc tttctgtgcc
    64021 cgtgcgcagc ggttcggaag tggggttccc gcagagcctg ggggaggttc acgatatgtt
    64081 gtacgataaa tcccttgaga gggataactg tggtttcggc ctgatcgccc acatagaagg
    64141 cgaacctagc cacaaggtag tgcgtactgc aatacacgca ctggcccgca tgcagcaccg
    64201 tggcgcgatt ctcgccgatg gtaaaaccgg cgacggttgc ggcttgctgt tacaaaaacc
    64261 ggatcgcttt tttcgcatcg ttgcgcagga gcgcggctgg cgtttagcaa aaaactacgc
    64321 tgtcgggatg ctcttcctga ataaagatcc tgaactcgcc gctgccgcac gccgcatcgt
    64381 tgaagaagaa ctgcaacgcg aaaccttgtc gattgtgggc tggcgtgatg tccccactaa
    64441 cgaaggcgtg ctgggtgaaa tcgccctctc ctctctgcca cgcattgagc aaatttttgt
    64501 gaacgccccg gcaggctggc gtccacgcga tatggagcgc cgtctgttta tcgcccgccg
    64561 ccgcattgaa aagcgtctcg aagccgacaa agacttctac gtctgtagcc tgtcgaatct
    64621 ggtgaacatc tataaaggtc tgtgtatgcc ggcggatctg ccgcgctttt atctggatct
    64681 tgcggacctg cgtctggaat cggccatttg cctgttccac cagcgcttct ccactaacac
    64741 cgtaccgcgc tggccgctgg cgcaaccgtt ccgctatctg gcgcataacg gtgaaatcaa
    64801 caccatcacc ggtaaccgcc aatgggcgcg tgcgcgtacc tataaattcc agacgccgct
    64861 gatccctgac ctgcacgacg ccgcgccatt cgtcaacgaa accggttctg actccagttc
    64921 catggataac atgctggaac tgctgctggc aggcgggatg gatatcatcc gcgccatgcg
    64981 cctgttagtg ccacccgcct ggcagaacaa cccggatatg gacccggaac tgcgtgcctt
    65041 ctttgacttt aactccatgc atatggagcc gtgggatggt ccggcgggca tcgtgatgtc
    65101 cgatggtcgc ttcgctgcct gtaacctcga ccgtaacggt ctgcgtccgg cacgctacgt
    65161 cattaccaaa gacaagctca tcacctgcgc ctctgaagtc ggtatctggg attaccagcc
    65221 tgacgaagtg gtcgaaaaag gccgcgtcgg gccaggcgaa ctgatggtga tcgacactcg
    65281 cagtgggcgt attctgcact cggcagaaac cgatgacgat ctgaaaagcc gccaccctta
    65341 taaagagtgg atggagaaaa acgtccgccg actggtaccg tttgaagatc tgcccgatga
    65401 agaagtgggt agccgcgaac tggacgacga cacgcttgcc agctaccaga aacagtttaa
    65461 ctacagcgcg gaagagctgg actccgtaat tcgcgtactg ggcgaaaacg gtcaggaagc
    65521 ggtcggttcg atgggcgatg ataccccatt cgccgtgctc tccagtcagc cgcgcattat
    65581 ttacgactac ttccgccagc agtttgccca ggtgactaac ccgccaatcg acccgctgcg
    65641 tgaagcgcat gttatgtcgc tcgccaccag tatcggtcgt gaaatgaacg tcttttgcga
    65701 agcagagggc caggcgcacc gtttaagctt taaatcgccg attctgctct actccgattt
    65761 caaacagctc acgacgatga aagaggagca ctaccgcgca gatacgctgg atatcacctt
    65821 tgacgtcact aaaaccacgc tcgaagcgac agtcaaagag ctatgcgaca aagccgaaaa
    65881 aatggtacgt agcggcaccg tgttgctggt gctctccgac cggaatatcg ctaaagatcg
    65941 cctgccggtt ccggctccga tggcggttgg cgcgatccag acccgtctgg tcgatcaaag
    66001 cctgcgttgc gatgccaaca tcatcgtcga aaccgccagc gcccgcgatc cgcaccactt
    66061 cgccgtgttg ctgggtttcg gcgcgacggc tatttatccg taccttgcct atgaaacgct
    66121 gggccgcctg gtagacaccc acgcgattgc caaagattat cgtaccgtga tgctcaacta
    66181 ccgtaacggc atcaacaaag gcttgtacaa aatcatgtcc aaaatgggca tctccaccat
    66241 cgcctcttac cgctgctcga aactgtttga agcggtcggt ctacacgatg atgtagtggg
    66301 cctgtgcttc cagggcgcgg tcagccgcat tggtggggca agctttgaag acttccagca
    66361 ggatctgctg aatctgtcga aacgtgcctg gctggcgcgc aagccgatta gccagggcgg
    66421 cctgctgaaa tacgtccacg gcggcgaata ccacgcctac aacccggacg tggtgcgcac
    66481 gctgcaacag gcggtacaaa gcggcgagta cagcgactat caggaatacg cgaagctggt
    66541 taatgagcgt ccggcaacca cgctgcgcga tctgctggca attacgccgg gtgaaaacgc
    66601 ggtcaacatt gctgatgttg aaccggcaag cgaactgttt aaacgctttg ataccgccgc
    66661 gatgtctatc ggcgcgttaa gcccggaagc ccacgaggcg ctggcggaag cgatgaatag
    66721 catcggcggt aactcgaact ccggtgaagg cggcgaagac ccggcgcgct atggcactaa
    66781 caaagtgtcg cgcatcaagc aggtggcttc tggtcgcttc ggcgttactc cggcgtatct
    66841 ggtcaatgcc gacgtcattc agattaaagt cgcccagggc gcgaagccgg gcgaaggtgg
    66901 tcagttgccg ggcgacaaag tcactcctta catcgccaaa ctgcgctatt cggtgcccgg
    66961 agtgacgctg atctccccgc cgccgcacca cgatatctac tctatcgaag acttagcgca
    67021 gcttattttc gacctcaagc aggttaaccc gaaagcgatg atctccgtta agctggtgtc
    67081 cgaaccaggc gttggcacca tcgcgactgg cgtggcgaaa gcttatgcgg acttgatcac
    67141 catcgcgggc tatgacggcg gcaccggcgc aagcccgctg tcgtcagtga aatatgcagg
    67201 ctgcccgtgg gaactcggtc tggtggaaac ccaacaggcg ctggttgcca acggcttgcg
    67261 tcataaaatt cgtttgcagg ttgatggcgg cctgaaaacg ggtgtcgata tcatcaaagc
    67321 ggcgattctc ggcgcagaaa gcttcggctt cggcactggc ccaatggtgg cgctcggctg
    67381 taaatatctg cgtatttgcc acctgaacaa ctgcgccacg ggtgtagcaa ctcaggatga
    67441 caaactgcgt aagaatcact atcacggcct gccgttcaag gtgactaatt actttgagtt
    67501 tatcgcccgt gaaacccgcg agctgatggc acagctggga gtaacgcgtc tggtggatct
    67561 gattggtcgc accgacctgc tgaaagagct ggacggtttc accgccaaac agcagaaact
    67621 ggcgctgtcg aagctgctgg agactgccga gccacatgca ggtaagtcac tctactgcac
    67681 tgaaaacaac ccgccgtttg ataacggcct actgaacgcg cagttgctgc aacaggcgaa
    67741 accgtttgtc gatgaacgcc agagcaaaac cttctggttc gatatccgca acaccgaccg
    67801 ttctgtcggc gcgtcgcttt caggctatat cgcccagacg cacggcgatc aggggctggc
    67861 agccgatcct atcaaagcgt acttcaacgg caccgcaggc cagagtttcg gtgtgtggaa
    67921 cgcgggcggc gtggaactgt acctgaccgg ggatgccaac gactatgtcg gtaaaggcat
    67981 ggcgggcggc ttaatcgcca ttcgtcctcc ggttggttcc gccttccgca gccatgaagc
    68041 aagcattatc ggcaacacct gcctgtatgg cgcgaccggt ggtcgtctgt atgccgcagg
    68101 ccgcgcgggt gaacgtttcg gcgtgcggaa ctccggtgct atcaccgtgg tagaaggcat
    68161 tggcgacaac ggctgtgaat atatgacggg cggtattgtc tgtattctgg gtaaaaccgg
    68221 cgttaacttc ggcgcgggca tgaccggcgg cttcgcttac gttctcgatg aaagcggtga
    68281 tttccgcaaa cgcgttaacc cggaactggt cgaagtctta agcgtcgacg atctggcgat
    68341 ccatgaagag catctgcgcg gtcttatcac cgagcatgtt cagcataccg gttctcagcg
    68401 cggtgaagag attctggcga actggtcaac cttcgccacg aaattcgcgc tggttaaacc
    68461 gaagtccagt gatgtaaaag cactgctggg tcaccgtagt cgtagcgcag cagagttgcg
    68521 cgtgcaggcg cagtaagggg tagcaacaat gagtcagaat gtttatcaat ttatcgacct
    68581 gcagcgcgtt gatccgccaa agaaaccgct gaagatccgt aaaattgagt ttgttgaaat
    68641 ttacgagccg ttttccgaag gccaggccaa agcgcaggct gaccgctgtc tgtcgtgcgg
    68701 caacccatac tgcgagtgga aatgcccggt acacaactac atcccaaact ggctgaagct
    68761 cgccaacgag gggcgtattt ttgaagcggc agaactgtcg catcagacca acacactgcc
    68821 ggaagtatgc ggacgtgtct gcccgcaaga ccgtctgtgc gaaggttcct gcaccctgaa
    68881 cgatgagttc ggtgcggtga ccatcggcaa cattgagcgt tatatcaatg ataaagcgtt
    68941 cgaaatgggc tggcgtccgg atatgtccgg cgtgaagcaa accggtaaaa aagtggcgat
    69001 tatcggtgca ggcccggcag gtctggcgtg tgcggatgtc ctgacgcgta acggcgtaaa
    69061 agccgttgtc ttcgaccgtc atccagaaat tggcgggctg ctgaccttcg gtattccggc
    69121 cttcaagctg gaaaaagagg taatgacgcg tcgccgtgaa atcttcaccg gcatgggtat
    69181 tgaattcaaa ctcaataccg aagtgggccg cgacgtacag ctggacgatc tgctgagtga
    69241 ttacgatgcc gtgttccttg gcgttgggac ttatcagtca atgcgcggcg ggctggaaaa
    69301 cgaagacgcc gatggcgtgt acgcagcgct gccgttcctt atcgccaaca ccaaacagtt
    69361 aatgggcttt ggtgaaaccc gcgacgaacc gttcgtcagc atggaaggca aacgcgtggt
    69421 agtacttggc ggtggcgaca ctgcgatgga ctgcgtgcgc acgtctgtac gccagggggc
    69481 aaagcacgtt acctgtgcct atcgtcgtga tgaagagaac atgccgggtt cccgccgcga
    69541 agtgaaaaac gcgcgggaag aaggcgtaga gttcaaattc aacgtccagc cactggggat
    69601 tgaagtgaac ggtaacggca aagtcagcgg cgtaaaaatg gtacgtaccg aaatgggcga
    69661 accggatgcc aaaggccgtc gccgcgcgga gatcgttgca ggttccgaac atatcgttcc
    69721 ggcagatgcg gtgatcatgg cgtttggttt ccgtccacac aacatggaat ggctggcaaa
    69781 acacagcgtc gagctggatt cacaaggccg catcatcgct ccggaaggca acgacaacgc
    69841 tttccagacc agcaacccga aaatctttgc tggcggcgat atcgtccgtg gttccgatct
    69901 ggtcgttacc gccatcgccg aaggtcgcaa agcggcagac ggcattatga actggctgga
    69961 agtttaagct ctgtcccagc gccgcctcct cagaaaagag tcggcgctga aattctcctt
    70021 tctttctgga aatcgcctcg cataatctga agaaaaccaa tgtaatacgc tcatctggat
    70081 tgttaccggt agatgcccgg cgtatagctg attccattat tcaacgcagc gccatataaa
    70141 gggacagata atggaatcgc tctcagagag aacctcaaca ggctaccagc aaatccacaa
    70201 cggcattatt catctggtcg atagcgcccg aacggaaacg gtacgtagcg ttaacgcgct
    70261 gatgaccgcg acgtactggg aaattggccg acggattgtc gaatttgaac aaggtggtga
    70321 ggccagagca gcttatggtg cacagttgat tgatcggcta tcgaaggatt taagtcgacg
    70381 gtataaacgt ggggtctctg caaaaaactt acgccaaatg aggctttttt acctcttttt
    70441 tcaacatgtt gaaattcgcc agacagtgtc tggcgaatta acaccattgg gaattccgca
    70501 gacaccatct gcagaattcc catcggcaaa gatttggcag acgctgtctg ccaaattgag
    70561 aggctgtttg atatttccac agcttttgta ttgcatctcc tattttcggt ttgtatattc
    70621 atgcaaataa acgaag
//
LOCUS       DAJTUI010000007        67332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTUI010000007 DAJTUI010000000
VERSION     DAJTUI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.007"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..218
                     /locus_tag="OO464_000517"
     CDS             <1..218
                     /locus_tag="OO464_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR6225783.1"
                     /translation="DSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGD
                     ARINASYTQLEIKHSQPKNRASGKRVS"
     gene            613..1252
                     /locus_tag="OO464_000518"
                     /pseudo
     CDS             613..1252
                     /locus_tag="OO464_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1355..1678
                     /locus_tag="OO464_000519"
     CDS             1355..1678
                     /locus_tag="OO464_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR6225784.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1745..3202)
                     /gene="nuoN"
                     /locus_tag="OO464_000520"
     CDS             complement(1745..3202)
                     /gene="nuoN"
                     /locus_tag="OO464_000520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6225785.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3209..4738)
                     /gene="nuoM"
                     /locus_tag="OO464_000521"
     CDS             complement(3209..4738)
                     /gene="nuoM"
                     /locus_tag="OO464_000521"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6225786.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4902..6743)
                     /gene="nuoL"
                     /locus_tag="OO464_000522"
     CDS             complement(4902..6743)
                     /gene="nuoL"
                     /locus_tag="OO464_000522"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6225787.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6740..7042)
                     /gene="nuoK"
                     /locus_tag="OO464_000523"
     CDS             complement(6740..7042)
                     /gene="nuoK"
                     /locus_tag="OO464_000523"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6225788.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7039..7593)
                     /gene="nuoJ"
                     /locus_tag="OO464_000524"
     CDS             complement(7039..7593)
                     /gene="nuoJ"
                     /locus_tag="OO464_000524"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6225789.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7605..8147)
                     /gene="nuoI"
                     /locus_tag="OO464_000525"
     CDS             complement(7605..8147)
                     /gene="nuoI"
                     /locus_tag="OO464_000525"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6225790.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8162..9139)
                     /gene="nuoH"
                     /locus_tag="OO464_000526"
     CDS             complement(8162..9139)
                     /gene="nuoH"
                     /locus_tag="OO464_000526"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6225791.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9136..11862)
                     /gene="nuoG"
                     /locus_tag="OO464_000527"
     CDS             complement(9136..11862)
                     /gene="nuoG"
                     /locus_tag="OO464_000527"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6225792.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11915..13252)
                     /gene="nuoF"
                     /locus_tag="OO464_000528"
     CDS             complement(11915..13252)
                     /gene="nuoF"
                     /locus_tag="OO464_000528"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6225793.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13249..13749)
                     /gene="nuoE"
                     /locus_tag="OO464_000529"
     CDS             complement(13249..13749)
                     /gene="nuoE"
                     /locus_tag="OO464_000529"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6225794.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13752..15554)
                     /gene="nuoC"
                     /locus_tag="OO464_000530"
     CDS             complement(13752..15554)
                     /gene="nuoC"
                     /locus_tag="OO464_000530"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6225795.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15648..16310)
                     /gene="nuoB"
                     /locus_tag="OO464_000531"
     CDS             complement(15648..16310)
                     /gene="nuoB"
                     /locus_tag="OO464_000531"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6225796.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16326..16769)
                     /gene="nuoA"
                     /locus_tag="OO464_000532"
     CDS             complement(16326..16769)
                     /gene="nuoA"
                     /locus_tag="OO464_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6225797.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17400..18338)
                     /gene="lrhA"
                     /locus_tag="OO464_000533"
     CDS             complement(17400..18338)
                     /gene="lrhA"
                     /locus_tag="OO464_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6225798.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19257..20474
                     /gene="alaA"
                     /locus_tag="OO464_000534"
     CDS             19257..20474
                     /gene="alaA"
                     /locus_tag="OO464_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6225799.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20558..21157
                     /gene="yfbR"
                     /locus_tag="OO464_000535"
     CDS             20558..21157
                     /gene="yfbR"
                     /locus_tag="OO464_000535"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6225800.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21216..23048)
                     /locus_tag="OO464_000536"
     CDS             complement(21216..23048)
                     /locus_tag="OO464_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR6225801.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23135..23785)
                     /gene="hxpA"
                     /locus_tag="OO464_000537"
     CDS             complement(23135..23785)
                     /gene="hxpA"
                     /locus_tag="OO464_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR6225802.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23796..24290)
                     /locus_tag="OO464_000538"
     CDS             complement(23796..24290)
                     /locus_tag="OO464_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6225803.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24373..24828)
                     /locus_tag="OO464_000539"
     CDS             complement(24373..24828)
                     /locus_tag="OO464_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6225804.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25166..26368
                     /gene="ackA"
                     /locus_tag="OO464_000540"
     CDS             25166..26368
                     /gene="ackA"
                     /locus_tag="OO464_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6225805.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26443..28587
                     /gene="pta"
                     /locus_tag="OO464_000541"
     CDS             26443..28587
                     /gene="pta"
                     /locus_tag="OO464_000541"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6225806.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28777..30297
                     /gene="yfcC"
                     /locus_tag="OO464_000542"
     CDS             28777..30297
                     /gene="yfcC"
                     /locus_tag="OO464_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6225807.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMSLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30330..30872)
                     /gene="yfcD"
                     /locus_tag="OO464_000543"
     CDS             complement(30330..30872)
                     /gene="yfcD"
                     /locus_tag="OO464_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6225808.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30930..31481)
                     /gene="yfcE"
                     /locus_tag="OO464_000544"
     CDS             complement(30930..31481)
                     /gene="yfcE"
                     /locus_tag="OO464_000544"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6225809.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31537..32181)
                     /gene="yfcF"
                     /locus_tag="OO464_000545"
     CDS             complement(31537..32181)
                     /gene="yfcF"
                     /locus_tag="OO464_000545"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6225810.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32317..32964
                     /gene="yfcG"
                     /locus_tag="OO464_000546"
     CDS             32317..32964
                     /gene="yfcG"
                     /locus_tag="OO464_000546"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR6225811.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33021..33383
                     /gene="folX"
                     /locus_tag="OO464_000547"
     CDS             33021..33383
                     /gene="folX"
                     /locus_tag="OO464_000547"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6225812.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33404..34297
                     /locus_tag="OO464_000548"
     CDS             33404..34297
                     /locus_tag="OO464_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6225813.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34345..35235)
                     /gene="rpnB"
                     /locus_tag="OO464_000549"
     CDS             complement(34345..35235)
                     /gene="rpnB"
                     /locus_tag="OO464_000549"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6225814.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35432..36205)
                     /gene="hisP"
                     /locus_tag="OO464_000550"
     CDS             complement(35432..36205)
                     /gene="hisP"
                     /locus_tag="OO464_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR6225815.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36213..36929)
                     /locus_tag="OO464_000551"
     CDS             complement(36213..36929)
                     /locus_tag="OO464_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6225816.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36926..37612)
                     /gene="hisQ"
                     /locus_tag="OO464_000552"
     CDS             complement(36926..37612)
                     /gene="hisQ"
                     /locus_tag="OO464_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6225817.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37702..38484)
                     /gene="hisJ"
                     /locus_tag="OO464_000553"
     CDS             complement(37702..38484)
                     /gene="hisJ"
                     /locus_tag="OO464_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6225818.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38705..39487)
                     /gene="argT"
                     /locus_tag="OO464_000554"
     CDS             complement(38705..39487)
                     /gene="argT"
                     /locus_tag="OO464_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6225819.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39753..40322)
                     /gene="ubiX"
                     /locus_tag="OO464_000555"
     CDS             complement(39753..40322)
                     /gene="ubiX"
                     /locus_tag="OO464_000555"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6225820.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40417..41934)
                     /gene="purF"
                     /locus_tag="OO464_000556"
     CDS             complement(40417..41934)
                     /gene="purF"
                     /locus_tag="OO464_000556"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6225821.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41971..42459)
                     /gene="cvpA"
                     /locus_tag="OO464_000557"
     CDS             complement(41971..42459)
                     /gene="cvpA"
                     /locus_tag="OO464_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6225822.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42718..43380)
                     /gene="dedD"
                     /locus_tag="OO464_000558"
     CDS             complement(42718..43380)
                     /gene="dedD"
                     /locus_tag="OO464_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6225823.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43370..44638)
                     /gene="folC"
                     /locus_tag="OO464_000559"
     CDS             complement(43370..44638)
                     /gene="folC"
                     /locus_tag="OO464_000559"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6225824.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44708..45622)
                     /gene="accD"
                     /locus_tag="OO464_000560"
     CDS             complement(44708..45622)
                     /gene="accD"
                     /locus_tag="OO464_000560"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6225825.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45778..46437)
                     /locus_tag="OO464_000561"
     CDS             complement(45778..46437)
                     /locus_tag="OO464_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6225826.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46520..47332)
                     /gene="truA"
                     /locus_tag="OO464_000562"
     CDS             complement(46520..47332)
                     /gene="truA"
                     /locus_tag="OO464_000562"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6225827.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47332..48345)
                     /locus_tag="OO464_000563"
     CDS             complement(47332..48345)
                     /locus_tag="OO464_000563"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6225828.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48411..49547)
                     /gene="pdxB"
                     /locus_tag="OO464_000564"
     CDS             complement(48411..49547)
                     /gene="pdxB"
                     /locus_tag="OO464_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6225829.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGLLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49646..50641
                     /gene="flk"
                     /locus_tag="OO464_000565"
     CDS             49646..50641
                     /gene="flk"
                     /locus_tag="OO464_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6225830.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLTAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50638..51816)
                     /locus_tag="OO464_000566"
     CDS             complement(50638..51816)
                     /locus_tag="OO464_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6225831.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52071..53312)
                     /gene="fabB"
                     /locus_tag="OO464_000567"
     CDS             complement(52071..53312)
                     /gene="fabB"
                     /locus_tag="OO464_000567"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6225832.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53471..55477
                     /gene="mnmC"
                     /locus_tag="OO464_000568"
     CDS             53471..55477
                     /gene="mnmC"
                     /locus_tag="OO464_000568"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6225833.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55598..55876)
                     /locus_tag="OO464_000569"
     CDS             complement(55598..55876)
                     /locus_tag="OO464_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6225834.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55910..56458)
                     /gene="epmC"
                     /locus_tag="OO464_000570"
     CDS             complement(55910..56458)
                     /gene="epmC"
                     /locus_tag="OO464_000570"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6225835.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56458..57267)
                     /locus_tag="OO464_000571"
     CDS             complement(56458..57267)
                     /locus_tag="OO464_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6225836.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57267..58091)
                     /gene="mepA"
                     /locus_tag="OO464_000572"
     CDS             complement(57267..58091)
                     /gene="mepA"
                     /locus_tag="OO464_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6225837.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58095..59180)
                     /gene="aroC"
                     /locus_tag="OO464_000573"
     CDS             complement(58095..59180)
                     /gene="aroC"
                     /locus_tag="OO464_000573"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6225838.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59215..60147)
                     /gene="prmB"
                     /locus_tag="OO464_000574"
     CDS             complement(59215..60147)
                     /gene="prmB"
                     /locus_tag="OO464_000574"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6225839.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60313..60864
                     /gene="smrB"
                     /locus_tag="OO464_000575"
     CDS             60313..60864
                     /gene="smrB"
                     /locus_tag="OO464_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6225840.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60968..61858)
                     /locus_tag="OO464_000576"
     CDS             complement(60968..61858)
                     /locus_tag="OO464_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6225841.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61845..62369)
                     /locus_tag="OO464_000577"
     CDS             complement(61845..62369)
                     /locus_tag="OO464_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6225842.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62366..62836)
                     /locus_tag="OO464_000578"
     CDS             complement(62366..62836)
                     /locus_tag="OO464_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6225843.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62833..63381)
                     /locus_tag="OO464_000579"
     CDS             complement(62833..63381)
                     /locus_tag="OO464_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6225844.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63386..64108)
                     /locus_tag="OO464_000580"
                     /pseudo
     CDS             complement(63386..64108)
                     /locus_tag="OO464_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64128..64850)
                     /locus_tag="OO464_000581"
     CDS             complement(64128..64850)
                     /locus_tag="OO464_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6225845.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64910..66064)
                     /gene="yfcU"
                     /locus_tag="OO464_000582"
                     /pseudo
     CDS             complement(64910..66064)
                     /gene="yfcU"
                     /locus_tag="OO464_000582"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66013..66768)
                     /locus_tag="OO464_000583"
     CDS             complement(66013..66768)
                     /locus_tag="OO464_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6225846.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66850..>67332)
                     /locus_tag="OO464_000584"
     CDS             complement(66850..>67332)
                     /locus_tag="OO464_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6225847.1"
                     /translation="NNGTARFYGTIEDSVCSIVPDDHKLEVDMGDIGAEKLKNNGTTT
                     PKSFQIRLQDCVFDTQETMTTTFTGTVSSANSGNYYTIFNTDTGAAFNNVSLAIGDSL
                     GTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFNAWLVGAADAPDLGNFEANTTFQITYL
                     "
BASE COUNT        16807 a        18219 c        16723 g        15583 t
ORIGIN      
        1 ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa aaatattcag
       61 cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat tgttatttag
      121 ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata aaacactcac
      181 aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa tcaattcgac
      241 cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc actgactgat
      301 gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg cggcacgcga
      361 ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct cagatttctg
      421 ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat tcctcacaga
      481 aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata cctctaccaa
      541 gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaatgac gccttttatt
      601 gataatttac atatgaaaaa attttccttt tgttatatta tttctgtttt cactccctct
      661 gattattttt accaaccctg ggtcactgca ttatcgccaa cgccccgaca cgtaagtcct
      721 gagcaattag aaaaaacagt tcgttatctt acacaaactg tgcatccacg taatgccggc
      781 aatattgata atctgaatag gtccgcagag tatataaaag agttctttgt cagtagcggt
      841 gccagagtta ccgcgcaggg cgtccccgat tggctattac gactctgcgc ctggaagcca
      901 gaattaccct tacccggcaa tgtcctggct ttatcccgat cggggagact ttattgctgt
      961 ggtcggcaga atgcaggata tcaacgccgt tcgtcaggta aaagcggcat tgttgtcatc
     1021 ccgggattta tctgtttatt ctatgaatgc cccagggttt attcccggta ttgatttttc
     1081 tgatcacctc aattattggc aacacgatat tcccgccgta atgattactg acaccgcttt
     1141 ttatcgtaat aaacaatacc acttgcccgg tgataccgca gacagattga attatcagaa
     1201 aatggctcag gtggtagatg gtgttataac cttgttatac aacagtaaat aataaaaatt
     1261 tctcgaggcg ggtccagaaa cagcgacatg tacattttat tttccctcgt ctgttttaag
     1321 atgcgaaaca taaaattcaa tggaggaaaa ataaatggga atgattggct attttgcaga
     1381 aattgactca gaaaagatta accaactgct tgagtctact gaaaaacctt tgatggataa
     1441 tatacatgac acgctttcag gattacgccg actggaatca ctggatcgaa gtgagctgcg
     1501 taaacagttt tccattaagc gactcaatga aatggagatt tatccgggag tgaccttttc
     1561 agaagagtta gaggggcaac tctttgcctc gatcatgctt gatatggaaa agctgattag
     1621 cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg ttgggtgatg
     1681 gtgtcagagc gccaaaaaaa accgccgaac atgtcggcgg ctttctgact tacaaactaa
     1741 cagtttacat cagcggcatt gctaaccgca caatactaat cagcggttgt ggccatacac
     1801 ccagcaccag taccaacagt gcagaaatca gcaccacgat accgcccgcg ctgtactgcc
     1861 agtttgacgg tgcatcgcga cccggttgtt ccggtgcgtg caggtacagg ctcaccgcca
     1921 cgcgcaggta gtagtagagg ccgattgccg aaccgacaac cacggcaccc accagccacc
     1981 acaagtgtgc ctggacaccg accgccagca cgtagaactt accgataaag cccagcgtca
     2041 tcgggatacc ggccagagac agcatcatca ccgtcatcac tgccgcgagg atcggacgat
     2101 gccagaacag accgcggtaa gagaacaggg aatcagcatc cgggccacga tacgggctgg
     2161 acatcaggct gaccacgccg aacgcgccga ggctgctgaa cagataaccg gccaggtaaa
     2221 cccctaccgc ttccatcgac atctcgccgg tttgcagcgc aatcagcgcc accagcagat
     2281 agccgaggtg agagatagat gagtaaccga gcagacgttt gatgttggtc tggctcaacg
     2341 ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagtact acgcgaatcg
     2401 cttcgctgtc acccaccggt gcgtagagga acagacgcat caccacaccg aagatagcga
     2461 ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg tatacgtctg
     2521 gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca atcatcatgc
     2581 cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccgaggttt ttacccagcg
     2641 cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccgaac agcaggaaag
     2701 aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagcgaa cgtttctggc
     2761 ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcaata ccgaggaaca
     2821 gagacgccag atggtttgca ttcgccagca ggatcccgcc cagcgcggca attaacacca
     2881 acaggtagaa ctcatccttg ttgtcgttat agccttcaag ccacgggtag gcgaaagtac
     2941 aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa ccatcaacgc
     3001 gcatcagcgg cgtaacgtcc atagcgcccg cctggccaac aaaccagagc gaaaccagcg
     3061 ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt cgccacgcaa
     3121 tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc agtgcgatca
     3181 ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg gaattaacaa
     3241 accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc tgcggataga
     3301 agcccagcag taccagcagc accaccagca acaggatcat aaacagctca cgcagcgaca
     3361 tccctggcag ttcctggctg gcaatctggc ttttcgcttt accgaagtaa gcgcgatgca
     3421 acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata acggtaatca
     3481 ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag ttaccggtgc
     3541 caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc catttcatct
     3601 tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca taaagctgac
     3661 cgcagagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg attaccgcgc
     3721 cctggtaggc caactggctg ccggtgtaga ttgcaatcag cacgaagccc atgtgggaaa
     3781 ccgaggtgta ggcgatcaga cgtttgatat cggtctgagc gaaggtcatc cacgcaccgt
     3841 agaagatgcc gataacaccc agccacatag cgattggcgc gaactctgcc gacgcgttcg
     3901 ggaacagcgg cagggagaaa cgcaacaaac cgtaagcggc ggttttcagc aagatccccg
     3961 cgaggtccac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc cagccatgca
     4021 gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatc aacagatatt
     4081 ccacaccact ggacattggc gtattcagca gctcttcata gttgaaggtc cagacgccgg
     4141 tcgcattgta gtgaacaaaa accagcgcca ggatggcgat caacatcacc agaccactcg
     4201 cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg tcagaggctt
     4261 tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc cagaagaaga
     4321 agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg atccacatca
     4381 ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat agtaccgcca
     4441 gcacaccgag cagaccggtc agcacgacca tcagcagcga cagaccgtca atggcgagat
     4501 gaatagagat accaaaacgc gggatccacg gcatgtcgaa ttcagactgc cactgcggaa
     4561 ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc gacagcgcca
     4621 gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca aagcgttcgg
     4681 tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt agtaacatgg
     4741 cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat tctcacgaca
     4801 gaaaattctc ttcggactgg gggtagtgtc ggatgcgccg cgaaggcagc ttatccgacc
     4861 tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag taccatcaac
     4921 agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg cagataaccg
     4981 ttctcgctta acagcagacc tttacctgca aagcgggaaa gaaccgccgg gatgttcatc
     5041 attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg cttgacgaac
     5101 actttgtcgt acagccagtc aaagccccaa gcgttgtacc accaggtact cagcaggcgg
     5161 cccggcgcac tgttggcgat ggaggtcacc agagaacgtt tacccagcca cagccaggct
     5221 gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa tttccagggt caacatgctg
     5281 ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac aatcagtgcg
     5341 ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt tacccctttc
     5401 acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat acggaaggtg
     5461 tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat atgaccattc
     5521 gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac cagcggcagt
     5581 gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat agatttacgc
     5641 agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac ggaaccggat
     5701 gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat cgccgcatcc
     5761 catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt agagtaagcg
     5821 agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag cagcgtaacc
     5881 gccccgacaa tacccaccag atgcagaact tccggcgtca tcaggaacag gccgtgggta
     5941 cgggcgatca ggtagacacc cgcggttacc atcgttgcgg cgtggatcag cgcggagaca
     6001 ggcgtcgggc ccgccatcgc gtcagcaagc catgtctgca acggcaactg cgcagattta
     6061 ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt gccgtcagca
     6121 aagtgcgctg gtgccagttc caccatatcg cggaagttca gggtgcccag ttcgttgtaa
     6181 agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac gaacgctttc
     6241 attgctgctg cgccattctt cggatcggtg taatagaaac cgatcagcag ataggagcac
     6301 aggcccacgc cttcccagcc gaggtacatc agcagcaggt tatcggcgag aaccagaact
     6361 accatgctgg cgatgaacag gttggtgtag gcgaagaagc gagagtagcc ctcttcaccg
     6421 cgcatgtacc aggaggcgta catgtgaata aggaaaccca caccagtgac caccgagagc
     6481 atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc gcctaccgac
     6541 atccacgtcc acagcggctg gctgtatgcc tgctcgccgt tagcgaagaa atcaacgccg
     6601 ataaaggcgg ttaccagcgc cgccaggccc acagagccaa cgccgacgat cgccgaaacg
     6661 ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa tggcaaaata
     6721 atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg ttcaggttct
     6781 ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct gccgccgcga
     6841 ggctgatggc gagaatgtac atcacctgac cgtcggtctg accccagtag cttccggcga
     6901 ccacaaaggc cagcgcggag gcgttaatca tgatttccag accaatcagc ataaacagca
     6961 gattgcgacg gataaccaga ccggttaagc caagaacgaa taagattgcc gcgaggatca
     7021 gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc tgtcgtcttt
     7081 acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg ccacaaccag
     7141 acccgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc cgaacagcgt
     7201 aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcgt taacaccgag
     7261 gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccgggc caatccacac
     7321 ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt tgagcatcat
     7381 caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt ccagcgcacc
     7441 ggcgaagtaa gcgcctagtg agaagaacac cccggaaatc gccagcagcg aaataatcag
     7501 gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg caagtatggc
     7561 tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg taacaggctc
     7621 ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc gtcgattgcc
     7681 atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga gatcagcaga
     7741 tcctctttct cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa atccggggtt
     7801 aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat gcagcgtgag
     7861 aagttaatgc ggaaaaattc cggataccag cgaccgtctt tggtttctgc tttttgcagc
     7921 gagatacagc cgaccgggca ggctaccgca cagaggttac aggctacgca acgctcttcg
     7981 ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag atagaccggc
     8041 tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat catccagata
     8101 ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt cttattgccc
     8161 cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc agcgtcagcg
     8221 gcaggcagat tttccagccg aaggacatta cccggtcata acgcggacgc ggtaacgacg
     8281 cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac cagatgaatg
     8341 gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat gcagagatgg
     8401 tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg gaatattcaa
     8461 tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg tgacggtgac
     8521 acaccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg atgacgttcc
     8581 acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc tgcgccacca
     8641 cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca gaagcacgca
     8701 tcgcacccag cagcgagtat ttgttgttac ttgaccagcc cgcaaacagc accgcgtaaa
     8761 ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg gcaaccaccc
     8821 aacccggact gactggcaca attgcaaagg ccagcagcag cgaggtaaag gcaatcatcg
     8881 gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct ttaaagaaca
     8941 ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg ttaggtccat
     9001 aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat gccccacagg
     9061 tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc tcaatcagtt
     9121 ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg cgccagccag
     9181 caccggagca atgccggaca tacccatcgg caagcccacc tgccctgccg tcagtccttc
     9241 ggcgatttca accggcagcg tgaccgtgtt gccatcgtaa ctaaaggaga cgcgtgtacc
     9301 tgcgttcaca cccaacttcg cggcatccgc tgggttgagt ttgatgtacg gctgcggcat
     9361 acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca ggtggtagta
     9421 cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc tggtgaagta
     9481 atccagaccg ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa agcgcagttt
     9541 gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc ccggcgccca
     9601 ggcaaacggc acttgcgaac ggtgcgcggt cggctggttg ttaccttcca tcgagaaggt
     9661 gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat tggcgcgcat
     9721 ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac gaatacggaa
     9781 tgtcgcatcc ggcgcagcat ctttgatacc cgccagttct gggattttcg ccacaacagc
     9841 gtcaattaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaagg tgctgtgcag
     9901 cgagtgcagc cagcgccagc tttccagcat gacagttttg ctgtcgtaat aagccggatc
     9961 gtaaacctgg aagaaacgtt gggcgcgacc ttcattatta attaccgtac cgtcgctttc
    10021 agcaaagctg gcagaggaaa gcaccagatg ggcgttttcc atgatcgctg tgcgttgatg
    10081 atcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg tagcagaagc
    10141 gtaacgatgc agatcgtttt ccaacaccac caccgcgtcg gcgcgtccgg tttccagttc
    10201 ggttaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc tgttgacgga
    10261 acgggcaatc atggtgatac caacgtcagc gccgcgacct ttcagggctt tcgcgacgtt
    10321 agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc ccggagataa tcaacggttt
    10381 cttcgcacct gccagtgcct gcacgatgac gtcgattttg ttttgcagct caggttcgat
    10441 accgtcaacc gctggtgcgg agttatccag cgcatgggcg atggcaaaac ctaaacgcgc
    10501 ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac gagtgtcatc
    10561 aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga ttgccgcaat
    10621 ctgccagtca gccactttct gtgctgcagc catttcgcgc gctttacctt tcaccgcctg
    10681 acgcactgcc agtgcgacgc gcgcgccggt ctgggtaacg tcttcgccca gcaccagtac
    10741 cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt cacgcagcac
    10801 tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt agaagttttc
    10861 ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg gagaaccaat
    10921 accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg cctgttcggc
    10981 gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac gatccttcag
    11041 gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt ttaccgtacc
    11101 gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc tgatgttaca
    11161 gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt tacggttgta
    11221 acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga ccaggttacc
    11281 ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt tgtcgtgcgc
    11341 gccataaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca cacagcggta
    11401 gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct gattacggtg
    11461 ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacgg tcatatcctg
    11521 aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt tggtcattaa
    11581 ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg aaataaaggt
    11641 gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt cttccgcgtt
    11701 ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg ccggatgcca
    11761 gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt tgtccgctcc
    11821 gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc ttccagtttt
    11881 ctcggtcagg gaccgagcgt taatcgaaat tcggttacca gcgctctttc agcaggttcg
    11941 gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgattccc gcctcaaatt
    12001 cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt gcgtgggcac
    12061 agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg atatcgcccg
    12121 gctggccttc gccacgctcc agcgcacgca gaattttcac gctccacggc agaccgtcgc
    12181 ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg ttacgcacca
    12241 gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc agacggctgc
    12301 ccgctttacc gatactttcg aattccatcg gcagatcaag gtgcgcttcg gtcaggaagt
    12361 cagtccccgc gccgcctggc tgccaggctt taaatttcag accatcacgc ataccaccgg
    12421 cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac agtcccggat
    12481 ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt ttcgagatgt
    12541 tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg gtttcgacgt
    12601 tgttgacaca ggtcggtttg ccccatacgc cggaggttgc cgggaagggg ggtttcgagc
    12661 gtgggttagc acgacgccct tccagggagt tgattaacgc tgtttcttcc ccgcagatgt
    12721 agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa acctgttccc ataatgtttt
    12781 tgccaagcaa gcccgcttca gtggcttcgg caatggcgcg gcgcagattt actgccgctt
    12841 cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca aacgcggaga
    12901 tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg tctttatagg
    12961 tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg gattcgtctt
    13021 tcggcatcag gctccacttc aggccagtcg aaaagcccgc gccgccgcgc cctttcagac
    13081 cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc gccttacgcg
    13141 ccccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt ttgtcatcgc
    13201 gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc atttataccg
    13261 ctccagcagt tcagggatcg cttccggggt cagatgcgcg tgagtgtcct catcgatcat
    13321 catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg taaagcggcc
    13381 atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg cctgaatacc
    13441 ctgatacccg ttgatatgac agaccacgct gtcgcaataa cggatcacat ggcgaccaac
    13501 cggctggcgg aagatctgac tgtagaacgt tgccacacct tcgacgtcgc ttgccggaat
    13561 acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac gctgcttctg
    13621 aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt gcatctcgtg
    13681 ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg gttgttgatt
    13741 ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc cagataaaca
    13801 atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa atgcgcataa
    13861 ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt caggtagtaa
    13921 ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt cattggcagg catcaccggc
    13981 ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag cgtgcgctct
    14041 ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg catgttgttg
    14101 aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttcag cattacgcgg
    14161 gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt ttcatagcca
    14221 gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag gcccgcgcca
    14281 gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg ggaacgacct
    14341 ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt cggcatccag
    14401 tcgaggaact cacgcagcag acgatcccag ccgcgcggca ggtcgtgcgc tacgccgcca
    14461 atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag atcgtaaatt
    14521 ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc ctgaataaag
    14581 gtcgagatgt acagcaggtg gctgttgata cggaacagtt cggagagcat aacgcgaatg
    14641 acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag cacgtaaggc
    14701 atttcgttaa cgcagccgcc gaggtattca atacggtcgg tatacggaat gtagctgtgc
    14761 caggactggc gctcgcccat tttctccgca ccacggtggt ggtagccgat gtctggtacg
    14821 cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc gtgcgccgac
    14881 gggtggttcg gaccgaggtt gaggaacatg aagtcctcat tttcggtgcc gcgcttcatc
    14941 ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt ggctttggtc
    15001 agctcaaacg gcgagaattc ggtagcgcgt gccgggtaat ctttacgcag cgggtgacct
    15061 ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa agtaatgcca
    15121 aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt ggtgaaggtc
    15181 ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg gttacgatcg
    15241 atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc gcggtgtgtg
    15301 cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt cggcagtttc
    15361 tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac gggaaccccg
    15421 gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag ttcgccaatt
    15481 accggatcat caagatgatc gcgggtctgc caggcgggtt cttgcgcggt taagtcggtc
    15541 atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc ttcgcgaaat
    15601 agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta aatctcgtca
    15661 ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg ctctgattgc
    15721 atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg acgttctttg
    15781 ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg cggcgggcag
    15841 cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac ggaataaata
    15901 tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt tggttccagc
    15961 atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt tcctgcaacc
    16021 accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa acgcgccacg
    16081 tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag accgaagtta
    16141 tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt gcccataaac
    16201 acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt ttgcaggggg
    16261 taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat cttaatgcct
    16321 cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg ctcgcggcgt
    16381 gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag acctgccagt
    16441 aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc gcggatagag
    16501 gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa catggccacc
    16561 agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat accggattca
    16621 aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc gcctaccagc
    16681 atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca gtgatgagcg
    16741 atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc gccagattca
    16801 ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa aaaaacctta
    16861 caataactgt agaaattctt ttatacagct aattgatgtg gtcttttact cctttctata
    16921 accttttgtc aactttaaca aaagcttctt cacattagtt tacataatat caacaccatt
    16981 agcatttaat gccctttcac cccagatcct tgacgactcc aggataatta gatgttgttg
    17041 aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag gaatttgtgt
    17101 cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt gttattccat
    17161 aacaaaaacg cagcaacaaa tttacgtatt ttttaacatc attgtagcag gtgatttttt
    17221 tcaggcgatt atttgtgcat tcgggacgtg aatctctggt gattgaaaaa taaacagttt
    17281 tgtacgttct gcactatgcg aggaaggatt tttactaaaa aaaagccgct ggggtttaaa
    17341 acacccccag cggctcgttt tttacactat tgtctcagga attatctatc gtccgtcgat
    17401 tactcgatat ccctttcaat caacaatgaa tcatcccctt ccggagcaga cattgggctg
    17461 tattgccacg gattatggta gctctccatc gcctgataga tcacctgggc cagctcatta
    17521 ttactggacg ggtcatagca cagcaaatat tcggtatcag gtagcggcgg taagccatct
    17581 actccactca gcacacgcag gtccgggctc atcatctcaa ccggccttgc cgtcacgcca
    17641 agaccggctt tcactgccgc acgaacggcc ggaagcgtcg aggcgacata agccagtcgc
    17701 catggaatat ctactttatt aagcgtcgcc agcaccatat cgcgaaacgg acttggatca
    17761 tccagcaata caagagggat cggctcgcct ttttgcaata cgtattccgc tgcgcagtac
    17821 cagtgtgttg gcgaggtacg caggttcaat gccttaaacg ctgaaggacg atgggtggtt
    17881 accatcaaat ccacttcctg ggattccagc atttctgcca tataggcatt acgtttaacg
    17941 cggacatcca gcgctaattt cggataaacc gaactcacgc gatttaacag gaaaggtaag
    18001 atcgtatcgg cagattcatc tgaagcaccg atagttaaca cgccctgaag attactgaac
    18061 attaatgatg agcaggcctc atcattaaaa cgcaggattt tcctggcgta accaagaagt
    18121 tgaatgccat gttcagttaa cagtttgttg cgaccgtgac gagcgaacag ttctttccca
    18181 acgagttgtt ccagacgctg catttgctga cttacggcgg actgagtacg acacacagcg
    18241 gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa caaatgttct cagcagatcg
    18301 aggtcgaggt taattatcgg acgatttgca cttatcatat attatcactt actggcggct
    18361 catactgagc tggttaatgc tgtgcacaca caaacaagca attccatttg taatgtgcct
    18421 ccctggcagt ttcatccgga acccggacga aagtaaaaat gcatatgagt tgcactaaaa
    18481 aagcgactca cattgttccg ttataatgcc tgaagtagat cacagaatat atcttcaggg
    18541 atcgcatatc tattaagtta ctcactcttt tctatttatg acatgcgcgt gtttgtataa
    18601 atgtaaatgt gagtccttgt tccactctcg tgcagcatcg ctggtcatac gcgaacacgt
    18661 acaaacagca atggtgaagc ggcatcaaga gcagggatcc gtcatttatg cgagcatttt
    18721 accccaaaaa ccttttattt ataagggtca ttgcgaatta tctgatgcaa agttatgtta
    18781 tgttaggcaa agtaatcttc tattattaat aagcacatca aaaccttttt gaatattaaa
    18841 taataattaa ttagcatcat cctcattcat taattccgct taacaatagt ttcacacctt
    18901 acccccattg cgacacctcg gaaatcatca cgctgttaaa ctctccgtaa tgcggaaata
    18961 tcatcatcgc gtttcattag tgaattctta aatgaggcat tttcacacaa ttatcttaca
    19021 gataaaaaaa ccagacttgc aattaagaat tagaacaatt cactatataa cattgcatgt
    19081 aaagcatata agcctcatta tttgtcatta ttaagttatt aacagcacaa tcgagccttc
    19141 ccctctggca aaatcttatt ctgcagacct tcaaaacacc gtcctggggg agtacattgt
    19201 tctaagctga cttccacggc agggagtggc gataacagca aaaaaggtca agattcatgt
    19261 cccccattga aaaatccagc aaattagaga atgtctgtta tgacatccgt ggtccggtgc
    19321 tgaaagaagc aaaacgcctg gaagaagaag gtaacaaggt actgaaactg aacatcggca
    19381 acccagcccc attcggtttt gacgcgccag atgaaatcct cgttgacgtg atacgcaacc
    19441 tgcctaccgc tcaagggtat tgcgattcca aagggcttta ctccgcgcgt aaagccatca
    19501 tgcagcacta ccaggctcgt ggcatgcgtg atgttaccgt ggaagatatt tacatcggca
    19561 atggtgtatc ggagcttatc gttcaggcaa tgcaggcatt gctgaacagc ggggacgaaa
    19621 tgttggttcc tgcaccagat tacccactct ggaccgcggc ggtttcgctt tccagcggta
    19681 aagcggtgca ttatctttgc gatgaatcct ctgactggtt cccggacctc gatgatattc
    19741 gcgctaaaat tacgcctcgt acgcgtggga ttgttattat caacccaaat aacccaaccg
    19801 gcgcggtata ttccaaagag cttttaatgg agattgtgga gattgcacgt cagcataatc
    19861 tcattatctt cgccgatgaa atttatgaca aaattctcta cgacgatgct gagcatcact
    19921 caattgcgcc gctggcacct gacctgctga ccattacctt taacggactg tcgaaaacgt
    19981 accgcgttgc aggcttccgt caggggtgga tggtgttgaa cgggccgaaa aaacacgcca
    20041 aaggctacat cgaaggtctg gaaatgctgg cttcaatgcg cctgtgtgct aacgttcctg
    20101 cgcaacacgc cattcagacc gcgctgggtg gttatcagag catcagtgaa tttattaccc
    20161 ctggcggtcg tctttatgag cagcgtaacc gcgcgtggga actgatcaac gatattccgg
    20221 gcgtttcctg cgtgaaacct cgtggtgcgc tgtatatgtt cccgaaaatc gacgccaaac
    20281 gctttaacat tcatgacgat cagaaaatgg tgctggattt cctgttgcag gaaaaagttc
    20341 tgttggtgca agggacggca ttcaactggc cgtggccgga tcacttccgc attgtcacgc
    20401 taccgcgtgt cgatgatatc gagctgtctt tgagcaagtt cgcgcgtttc ctttctggtt
    20461 atcatcagct gtaatcttaa tttcactgcc ggagattgca tccggcagtg ttatcccgcc
    20521 acaatgacct gatgatgtca tcatacgtaa ggtcactatg aaacagagcc atttttttgc
    20581 ccatctctcc cgcctgaaac tcattaaccg ctggccgcta atgcgcaacg tgcggacgga
    20641 aaatgtgtcc gaacacagtt tgcaggtagc gatggtcgcc catgcgctgg cagctatcaa
    20701 aaatcggaaa tttggcggta atgtcaacgc cgaacgtatc gctttactgg cgatgtacca
    20761 cgatgcctca gaagtgctca ccggcgatct ccctactccg gtgaaatact tcaatgcgca
    20821 aatcgctcag gagtacaagg ctattgaaaa aatcgctcag cagaaactgg tcgatatggt
    20881 tccggaagaa ctgcgggata tctttgcgcc gttaattgac gagcatgcat atagcgatga
    20941 agaaaaatcg ctggtgaaac aggcagatgc actgtgtgca tatctgaaat gtctggaaga
    21001 actcgcggcc ggaaataatg aattcttgct ggcaaaaacg cgactggaag cgacgcttga
    21061 agcgcgtcgc agccaggaga tggactactt catggaagta tttgttccca gcttccatct
    21121 ttcgctcgat gagattagcc aggattcacc gctgtaagca gccggagtct gcgtcgcatc
    21181 agtcaataag cgccggatgc gacatcaggc tcttgtcaaa acggaaacag catcgggatc
    21241 atcaccacac aaaccgccat cacgataatg gtgaacggta cccccaactt cacaaagtca
    21301 ctgaagctgt aatttcccgg acctaaaacc agggtgttaa caggtgaaga caccggtgtc
    21361 ataaaggcgg cggatgctgc catcgccacg accatcgcaa atggataagg cgacactccc
    21421 atcgttttgg cagcagccag cgcaatcgga gccatcaaca ccgccgtcgc ggtattagag
    21481 ataaacagcc caataaccgc cgacaagaca aacaaacacc ccagcatcat atgtggccca
    21541 taaccgccgc caatatccat cagccctttc accgccagcg cgacacctcc cgttttctgt
    21601 aatgccacag caaacggcat catcccaacg atcaaaataa tgctcggcca gtgaatggat
    21661 ttataggcgc tttcagcatc tatacagcgg aatttcccca tcagcaggca ggcgatgata
    21721 gcggcaacag gattaggaat ttcatttgtc agcattaacg ccaccattag caccagacag
    21781 aaaatggcat ggggtgcctg gctgtgcgcg ggcgatgctt cactcacctc ttccggtaag
    21841 ttcagcgcta cgaagtcgcg gccctgtttg gccagcatac cgatcagttt ccagttaccc
    21901 acaaccagga tgatatcgcc cagcagcaga ggctcatccg ccagcgaacc ttccagcgcc
    21961 acgccattgc gcttgagccc caccacattc agtccgtagc gggtacgaaa accaatttcg
    22021 cgcaccgatt taccaatcag ttctgactca ggaattaatg aaatctctgc catgcccaca
    22081 tcaagggcct ggtcagaaaa atactcgccg cgcagtacca tcggctccag caattgctca
    22141 ctacaaaatt gccggagatc gacatcagcc gcagacatat caataagcaa aacgtcacgc
    22201 gcgcgaaatt cagaaacccc attaacgttc acgataacgc gacgaaaacg ccgccagcgt
    22261 tcaacaccga tgacgttagc gccataacgc tcacgtaatt tgagatcatc cagccgttga
    22321 cctatcattg gcgatccggg gcgaatagcc agacgtcgcg cacgcccggt cagtcgatat
    22381 tcacggataa gatcgcgaaa ggttcgacgc gtccagcctt cgcgctgcgg ggtctgggta
    22441 tcccctttca gcatgaaacg catcactaac atatacaaaa tacccagcac cagcacgacc
    22501 aggccaatag gtgttacgct aaagaaactg aagccgtgat agccttcacg cagcaattca
    22561 ctgttgacta ccaggttcgg cggcgtcgcc accagcgtca tcatgccgct aatcagcccg
    22621 gcaaaactta acggcatcat cagacgtgac ggcgaagttt gcatacgcat ggcaacgctt
    22681 aacaccacgg gaataaagat agcgacaacg ctggttgaac tcataaacgc gccaagcccc
    22741 gcgacggtca gcatcaacaa aaccaacatt ttgatttcac tgttgcccgc cactttgacc
    22801 agccatgttc ccattacggt ggcaacaccg gtacggacca aaccatcgcc aataataaac
    22861 aaggcggcaa tcaggacaac gttaggatca gaaaagccgg aaaatacttc ggggaccgtc
    22921 agcgttccgc ttaatgcaaa cgcgacaata acaaacaaag cgaccgcatc catacgcact
    22981 ctgcccgtcg caaacaagac gatggcaacg gccagtaatg aaagaaccca aatcaattca
    23041 ccgttcacaa cttatccttg ttaattgagg gggatgactt gattctgcca taaaaaagcc
    23101 ccgacgagac ggggctaaat catgatcagg tgtttcactg aataataaca tcgccatttg
    23161 gctgtttggt cacagtaatt tgctccagac tgtggaggac caaatcgacc tcattcaggc
    23221 gcggggtatc tgccggagcg ttaaccgcaa tgacatggca acccgccgcc aggccagaaa
    23281 gcacgccagc gggagcatct tccaccacca cacactcctg cggcgcaagc cccagcagct
    23341 gcgcacctaa cagatacgca tcaggttctg gttttccgcg cttcactcgc tcagcggtta
    23401 caaacacctc tggtgcggga agccccgcta ttttatggcg cgctcgcgct accggcatgg
    23461 agccagaagt cacaatggcc cacggaatac ctgctttatt caaatgattg agtaaggcga
    23521 ttgccccagg aagcgcggta ataccttcgg tttccgtggc ctcgatttgc tccagacgcg
    23581 taaactcggc ggcaatatca gcctcggatt tgcccgccat aaaatggcgc aaagaggtga
    23641 tcgcctgttt accgtgaatg aaagccagca cctcttccgg cgctaaccca tgacgtctgg
    23701 cccagttgct ccacgcccgt tctaccgcag gcagggaatc caccagcgtt ccatcaagat
    23761 caaacagaaa acctttgcac cgcacgcggg cctcctcagg cgttgataat ttgattaatt
    23821 tcgttggcgc tcaaatggta ctgacgcggg caggcatgcc acacattaag catgcgctga
    23881 tatttttccc acattggcgt ctgggcgtta aaaccatgag tcccagcgtc aaaatgggta
    23941 tagcgccctt ccacattaac cataaagcgg acataaccga ggtaacgtgc ttcagtggcg
    24001 gcgtcaaagc cgaggaaggt gacacgacgt tcatcgatgg attgctggtc ctgcaaatta
    24061 gaccaagaaa catacaacgc atgatacata tccataatgt cgatgatagt gcggcaggtt
    24121 tcttctttca gctcgccaaa ctcgcgatcc aattcacgca tctgtaatcc gtaaccacgc
    24181 tcaataattg tttgcaggcg acggtaacgt tcagcatttg ccggatcgag catagtcatc
    24241 atcttgtact gattagacaa aataagacgt tgcgcgttgg tcatttccat tgttgactcc
    24301 tgtatcactc tactacggtg aaaaaaaaga aggctgagta tgccttcttt tatatgcgta
    24361 atcaggggtc aattacaaat catcaaggaa agttttatcc agttgtttga aggcgcgctt
    24421 aagcgtgtca gctaatgcct ggtaatcagg cttgccttca acgggtgcca acacctgtcc
    24481 agactcctgc aatttaccgc gaacttcata aaaccagtta aggattgcag ggggtaatgg
    24541 cgttacagaa cgcttgccca gccaccacaa tccctgcatg ggtaaactta aggcgaacag
    24601 tgcagtggca actgccggcc caagcagacc gcccagggca atctgccagc agagagtaaa
    24661 tacggcgatc ggcggcataa aacggatcgc ataacgcgtc atcttgataa cgcgattttc
    24721 gacaaagacc ggagcaaggc gtttttccag tggccacgtc tttgagtaat gctgtccgcg
    24781 gcgaaacaag ctaaaaaaat taacagaacg attatccggc gttgacatgc ttcacctcaa
    24841 cttcacatat aaagattcaa aaatttgtgc aaattcacaa ctcagcggga caacgttcaa
    24901 aacattttgt cttccatacc cactatcagg tatcctttag cagcctgaag gcctaagtag
    24961 tacatattca ttgagtcgtc aaattcatat acattatgcc attggctgaa aattacgcaa
    25021 aatggcatag actcaagata tttcttccat catgcaaaaa aaatttgcag tgcatgatgt
    25081 taatcataaa tgtcggtgtc atcatgcgct acgctctatg gctccctgac gtttttttag
    25141 ccacgtatca attataggta cttccatgtc gagtaagtta gtactggttc tgaactgcgg
    25201 tagttcttca ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt acctttctgg
    25261 tttagccgaa tgtttccacc tgcccgaagc acgtatcaaa tggaaaatgg acggcaataa
    25321 acaggaagcg gctttaggtg caggcgccgc tcacagcgaa gcgctcaact ttatcgttaa
    25381 tactattctg gcacaaaaac cagaactgtc tgcgcagctg actgctatcg gtcaccgtat
    25441 cgtacacggc ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg ttattcaggg
    25501 tatcaaagat gcagcttctt ttgcaccgct gcacaacccg gctcacctga tcggtatcga
    25561 agaagctctg aaatctttcc cacagctgaa agacaaaaac gttgctgtat ttgacaccgc
    25621 gttccaccag actatgccgg aagagtctta cctctacgcc ctgccgtaca acctgtacaa
    25681 agagcacggc atccgtcgtt acggcgcgca cggcaccagc cacttctatg taacccagga
    25741 agcggcaaaa atgctgaaca aaccggtaga agaactgaac atcatcacct gccacctggg
    25801 caacggtggt tccgtttctg ctatccgcaa cggtaaatgc gttgacacct ctatgggtct
    25861 gaccccgctg gaaggtctgg tcatgggtac ccgttctggt gatatcgatc cggcgatcat
    25921 cttccacctg cacgacaccc tgggcatgag cgttgacgca atcaacaaac tgctgaccaa
    25981 agagtctggc ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg ttgaagacaa
    26041 ctacgcgacg aaagaagacg cgaagcgcgc aatggacgtt tactgccacc gcctggcgaa
    26101 atacatcggt gcctacactg cgctgatgga tggtcgtctg gacgctgttg cattcactgg
    26161 tggtatcggt gaaaatgccg cgatggttcg tgaactgtct ctgggcaaac tgggcgtgct
    26221 gggctttgaa gttgatcatg aacgcaacct ggctgcacgt ttcggcaaat ctggtttcat
    26281 caacaaagaa ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac tggttatcgc
    26341 gcaagacgcg agccgcctga ctgcctgatt tcacaccgcc agctcagctg gcggtgctgt
    26401 tttgtaaccc gccaaatcgg cggtaacgaa agaggataaa ccgtgtcccg tattattatg
    26461 ctgatcccta ccggaaccag cgtcggtctg accagcgtca gccttggcgt gatccgtgca
    26521 atggaacgca aaggcgttcg tctgagcgtt ttcaaaccta tcgctcagcc gcgtaccggt
    26581 ggcgatgcgc ccgatcagac tacgactatc gtgcgtgcga actcttccac cacgacggcc
    26641 gctgaaccgc tgaaaatgag ctacgttgaa ggtctgcttt ccagcaatca gaaagatgtg
    26701 ctgatggaag agatcgtcgc aaactaccac gctaacacca aagacgctga agtcgttctg
    26761 gttgaaggtc tggtcccgac acgtaagcac cagtttgccc agtctctgaa ctacgaaatc
    26821 gctaaaacgc tgaatgcgga aatcgtcttc gttatgtctc agggcactga caccccggaa
    26881 cagctgaaag agcgtatcga actgacccgc aacagcttcg gtggtgccaa aaacaccaac
    26941 atcaccggcg ttatcgttaa caaactgaac gcaccggttg atgaacaggg tcgtactcgc
    27001 ccggatctgt ccgagatttt cgacgactct tccaaagcta aagtaaacaa tgttgatccg
    27061 gcgaagctgc aagaatctag cccgctgccg gttctcggcg ctgtgccgtg gagctttgac
    27121 ctgatcgcga ctcgtgcgat cgatatggct cgccacctga atgcgaccat catcaacgaa
    27181 ggcgacatca atactcgccg cgttaaatcc gtcactttct gcgcacgcag cattccgcac
    27241 atgctggagc acttccgtgc cggttctctg ctggtgactt ccgcagaccg tcctgacgtg
    27301 ctggtggccg cttgcctggc agccatgaac ggcgtagaaa tcggtgccct gctgctgact
    27361 ggcggctacg aaatggacgc gcgcatttct aaactgtgcg aacgtgcttt cgctaccggc
    27421 ctgccggtat ttatggtgaa caccaacacc tggcagacct ctctgagcct gcagagcttc
    27481 aacctggaag ttccggttga cgatcacgaa cgtatcgaga aagttcagga atacgttgct
    27541 aactacatca acgctgactg gatcgaatct ctgactgcca cttctgagcg cagccgtcgt
    27601 ctgtctccgc cagcgttccg ttatcagctg actgaactgg cgcgcaaagc gggcaaacgt
    27661 atcgttctgc cggaaggtga cgaaccgcgt accgttaaag cagccgctat ctgtgctgaa
    27721 cgtggtatcg caacttgcgt actgctgggt aatccggcag agatcaaccg tgttgcagcg
    27781 tctcagggtg tagaactagg tgcaggcatt gaaatcgttg atccagaagt ggttcgcgaa
    27841 agctatgttg gtcgtctggt cgagctgcgt aagaacaaag gcatgaccga aaccgttgcc
    27901 cgcgaacagc tggaagacaa cgtggtgctc ggtacgctga tgctggaaca ggatgaagtt
    27961 gatggtctgg tttccggtgc tgttcacact accgcaaaca ccatccgtcc gccgctgcag
    28021 ctgatcaaaa ctgcaccggg cagctccctg gtatcttccg tgttcttcat gctgctgccg
    28081 gaacaggttt acgtttacgg tgactgtgcg atcaacccgg atccgaccgc tgaacagctg
    28141 gcagaaatcg cgattcagtc cgctgattcc gctgcggcct tcggtatcga accgcgcgtt
    28201 gctatgctct cctactccac cggtacttct ggtgcaggta gcgacgtaga aaaagttcgc
    28261 gaagcaactc gtctggcgca ggaaaaacgt cctgacctga tgatcgacgg tccgctgcag
    28321 tacgacgctg cggtaatggc tgacgttgcg aaatccaaag cgccgaactc tccggttgca
    28381 ggtcgcgcta ccgtgttcat cttcccggat ctgaacaccg gtaacaccac ctacaaagcg
    28441 gtacagcgtt ctgccgacct gatctccatc gggccgatgc tgcagggtat gcgcaaaccg
    28501 gttaacgacc tgtcccgtgg cgcactggtt gacgatatcg tctacaccat cgcgctgact
    28561 gcgattcagt ctgcacagca gcagtaatct cgtcatcatc cacagctttg cgctgcggat
    28621 atctgaaccg gaaataatca ctatttccgg ttttttattc tcttaatttg cattaatcct
    28681 ttctgattat cttgcttaac tgcgctgcat caatgaattg cgccatctca ctttgcatac
    28741 ttaccacttt gttttgtgca agggaatatt tgcgctatgt ccgcaatcac tgaatccaaa
    28801 ccaacaagaa gatgggcaat gcccgatacg ttggtgatta tcttttttgt tgctatttta
    28861 accagtcttg ccacctgggt agttccggtg gggatgtttg acagtcagga agtgcagtat
    28921 caggttgatg gtcaaacaaa aacacgcaaa gtcgtagatc cacactcatt tcgcattctg
    28981 actaacgaag caggcgaacc tgagtatcac cgcgtacagc tgttcacgac gggcgatgaa
    29041 cgcccgggcc tgatgaactt cccgtttgaa gggttaacct caggatcgaa atacgggaca
    29101 gccgttggca tcatcatgtt tatgctggtg attggcggcg cgtttggcat tgtgatgcgt
    29161 acaggaacca ttgataacgg tatcctggcg cttattcgcc atacccgcgg gaatgaaatt
    29221 ctctttattc ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt tggtatggga
    29281 gaagaggccg tcgcctttgc cattatcatc gcaccgctaa tggtccggct gggctatgac
    29341 agtattacca ccgtcctggt gacttatatt gccacgcaaa tcggttttgc cagttcgtgg
    29401 atgaacccgt tttgtgtggt tgttgctcag gggattgccg gcgttccggt gctttctggc
    29461 tccgggttgc gcatcgtggt gtgggttatc gccactctga ttggcctgat ctttaccatg
    29521 gtgtacgcct cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga gtccgaccgc
    29581 ttctttcgtg aaaagcaggc ggatgttgaa caacgtccgt ttacctttgg tgactggctg
    29641 gtattgattg tcctgacagc cgtaatggtc tgggtgattt ggggcgtgat cgttaatgcc
    29701 tggtttattc cagaaattgc cagccagttc ttcaccatga gtctggtgat tggcatcatc
    29761 ggcgttgttt tccgccttaa cggcatgacg gttaatacca tggcttcatc ctttaccgaa
    29821 ggggcgcgaa tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg gattttgctg
    29881 ctggttggta atggtgaagc aggtaatgcc agcgtgttaa atactatcct caacagcatt
    29941 gccaatgcca ttagcggtct ggacaacgcg gtcgcggcct ggtttatgtt gctcttccag
    30001 gctgtattta atttcttcgt gacgtccggt tctggtcagg cggcattaac tatgccgtta
    30061 ctggcaccgc ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct ggcgttccag
    30121 tttggtgatg gcttcagcca catcatttac ccaacctcag cttcgttaat ggcaacgctc
    30181 ggtgtgtgcc gggtggactt ccgtaactgg ctgaaggtgg gcgcgaccct gcttggactg
    30241 ctgtttatta tgtccagcgt cgtagtgatc ggcgctcagt tgatgggcta ccactaaaaa
    30301 tgttaagagc cgcaattgcg gctctttttt cattctgccg tttcagtctc tacggcttca
    30361 tttttggcat tgcgcttcat ccacaacgcc agcgctttca gcgagtctgg agtgaactca
    30421 tcgcagcgtg cggtgatttc ttccggcgtc agccagcaaa ctccactgac ttcatcttcc
    30481 tgtagggcga agggaccgtg agagacgcaa ctgaacaacg cgccccagac acggcaattt
    30541 ttatcttcga aatagaactg cccgtgctcg gcaaagggga caccggcaat gcccaactct
    30601 tcttccgctt cgcgacgcgc ggattccagc agttgctcat cggcctgaac tacaccgcct
    30661 gcggtcgcat ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg ctggaccaga
    30721 attttgccca tgccatcatg cacgacgatg taagttgcac gatgacgcag acactgtgcc
    30781 cgcatttgtt cccggctggc ttgtgcaatg acttcgttct cttcattgac aatatccacc
    30841 cattcagtac ttgccaaacg acgctgttcc attatcagga aaccttcttt ttctggcgct
    30901 cttacggcgc attttgagtt gtgggtaaat tacggattaa tcgcgacctg cgcaatgata
    30961 ctttgatcat tgagtgcgat tacactaagt acgtcattat ccagcatgcc ataacccgcc
    31021 ggattaccgc ctttcggaat actcaccgag ccggggttga agtggaaaat ctccccccgc
    31081 tgttctgcca ctggcagatg ggtatgaccg tacaccagca catcgttctg gtttaaagca
    31141 ggtagatttt ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg tttttccagt
    31201 aatacctgtt gccacggcgc ggttatcggg aaatgcagca tcatttgatc cacttcgctg
    31261 tcgcagttgc cacgcacagc gataacctta tgtgccactt cattaagccg ttcagcgact
    31321 ttggctggcg cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa cacgtcgcca
    31381 aggatcacca gccactgggc accgctttgg acaaacaact ccacaacacg ttccgtcgcc
    31441 ggtaacgacc catgaatgtc cgatgcaaac atcagtttca tcactcactc ctcgtcgaaa
    31501 aaacgtaagc tatgatactg gattctgtcg ttgctatcag ccagattgct tcgccgaaag
    31561 tgcaataaaa cgctggacag acgctcgctg ccactggaat gtcgcataat ccaccaggcg
    31621 ttctggcacc tcatcgccat gtagtaccag gcggttaatc attagcgcca gatcagtatc
    31681 agcaatgcac cattcaccaa ataaattcgg ctgacccagt gctaacaaat gttctgccat
    31741 cgcgaacagt ttctctgcac tggcttttcc ctcggccgtt agtggcgctt ttttcgcccc
    31801 cgcaaagaca acatccgtcg gacgctcttc gcggatgggc atcagatcgc tgcgcagcca
    31861 ggcctgaatc tgtcgtgcac gcgcacgatt ttctaaatca agcggataaa tacgttccca
    31921 ggtcggtggc gcaaatcgat cttccagata ctccgcaatg gcagaagatt cactcaactc
    31981 aaaatcatcg atttgtaata acggcacacg gcgtgtctga ccgtaacctt gccacgtcgg
    32041 ctgcaaatgt tcaccgctgt cgaggtcgat ggtcttgata tgaaacgaca ggcctttttc
    32101 ctgcaacgcc acccaggcgg ataacacata aggggagaaa aagtgggcat ctgaccaaag
    32161 cgtgatagcg ggtttactca ttatgtcctc ggcaataaag ggggctggct atcaaactat
    32221 agagcctttt tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg ctttctatac
    32281 ttgtggtgtt cagatgatta aacaaccgga gctgcaatga tcgatctcta tttcgccccg
    32341 acacccaatg gtcacaaaat tacgctgttt ctcgaagaag cagagcttga ttatcgcttg
    32401 attaaggtag acttggggaa aggcggtcag tttcgtccgg aatttttgcg catttcgccc
    32461 aacaacaaaa ttccggcaat tgttgatcat tctcctgccg atggcggcga accgttaagc
    32521 ctctttgagg ctgctgccat tttgttgtat ctggctgaga aaacaggact ctttttgagt
    32581 catgaaacgc gtgagcgcgc cgccacatta cagtggttat tctggcaggt aggcggactg
    32641 gggccgatgc ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac cattccttac
    32701 gctattgaac gttatcaggt tgaaactcag cgtctttacc atgtactaaa caagcggctg
    32761 gaaaactcgc cctggctggg aggcgagaac tacagcattg cggatattgc ctgctggccg
    32821 tgggttaatg cctggactcg cctgcgaatt gacctcgcaa tgtatccggc agtcaagaac
    32881 tggcatgagc ggatccgttc gcgccctgcc accgggcagg cactgctaaa agcacaactc
    32941 ggtgatgagc gttcggatag ttaacagaaa caggttctcg tgtattattt catcctaagt
    33001 aaaacaacgg agaacctgca atggcacaac ctgccgctat tattcgtata aagaaccttc
    33061 gtttgcgtac gtttatcgga attaaggaag aagaaattaa caaccgtcag gatattgtta
    33121 tcaatgtgac gatccactac cccgccgata aagcgcgcac tagcgaagat atcaacgatg
    33181 cgctgaatta tcgcaccgta acgaaaaaca ttattcagca tgtagagaat aaccgtttct
    33241 ctttgctgga aaaattaact caggatgtgc tcgatatcgc acgtgaacat cactgggtga
    33301 cgtatgctga agtggagatc gataaactgc acgcgctgcg ctacgccgat tcggtatcca
    33361 tgaccttaag ctggcagcgt taatcgccat atcgggaggc tgcatgaaca tagtgatcac
    33421 cggagggacg ggattaattg gtcgccattt gattccgcgt ttgctggagc tgggccatca
    33481 aattacggta gtgacgcgta acccgcagaa agccagttcc gttctcggcc ctcgggtgac
    33541 actatggcaa gggcttgccg atcaaagcaa cctcaacggc gttgatgcgg taatcaacct
    33601 ggccggagaa ccgattgctg ataaacgctg gactcacaag caaaaagagc gtctctgcca
    33661 aagccgctgg aatatcacgc aaaaactggt cgatttgatt aatgctagcg acacgccgcc
    33721 gtcggtactc atttccggct cggcaacggg ctattatggc gacttaggtg aagtggtggt
    33781 taccgaagag gaaccgccgc ataacgaatt tactcataaa ctctgcgccc gctgggaaga
    33841 gattgcctgc cgggcgcaaa gtgacaaaac gcgagtgtgc ctgctgcgaa ccggcgtggt
    33901 actggcaccg gacggcggta ttctcggtaa aatgctgcca ccgtttcgtc ttggcctggg
    33961 cgggccgatt ggttccggtc ggcagtatct ggcctggatt catattgatg atatggtcaa
    34021 cggcattctc tggctgctgg ataacgagct gcgcgggcca tttaatatgg tttcgcccta
    34081 cccggtacgc aatgaacaat ttgcccatgc gctcggtcat gcgctgcatc gcccggctat
    34141 tttgcgcgtc cccacaaccg ccattcggct gttaatgggc gaatcttcag tactggtatt
    34201 aggtggacaa cgcgcgctgc ctaaacggct ggaagaagcg ggttttgcgt ttcgctggta
    34261 cgatttagaa gaggcgctgg cggatgtcgt tcgctgatgt ggtttacagc aaacatccgc
    34321 cagttaactc cctgtgttac aggattagtg gctttgcgcg ataagatcgt ctggtgaaag
    34381 tcgggtcacc atcataacta actctctgtc taaacctcta tccagcatct cctgagcaat
    34441 acgcagggct tcttcgtgtt tgccctgcat tgcgccttct tcacgtaatc tgtcagcaat
    34501 ggtcatcagt ttctcctttt cttgtggtgc gcgttctgtt atctcaccaa taaatgcacg
    34561 gaaacgctgg gcatcccctg tttgtaatac gtaattaaac agggctttta gctgtctgtc
    34621 attagtgttc cctgtaacta gcagcgaaac aatttggtcg actaatccca acagatcgcg
    34681 ctgacgaata tgtttctgaa ttaactccaa cagcgccatt ttgcggtgtt gcataatctc
    34741 gtcatccggc accacggtaa tatccaccaa cggaaacgct gatgaatata ttttgcgggc
    34801 tatagcaggc tcggcaaatt catcaagcca gcagagtgaa taaggataag gacttctgca
    34861 accatgataa aacagcatcg ggatcaccaa tggaagctct ttatagcccg catcaagatg
    34921 gttttgcatt gccgcaatgg aataacgcat catgcgaaaa gccattaatt cttccggctt
    34981 actttggtgc tctatcacta cataaatata acccactccc tcctgcgttt tcacagacca
    35041 caagaggtcg gaataatatt gccgcaggtc ttcatcaata aaactgtttg gttctagttt
    35101 aagcgtcgtt aaatcacaca gtttgcgcag cggcgcggga agatgaatat taataaaatt
    35161 ccgcgcggtg tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg gcgtggaagt
    35221 tgtcgatatt gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg acaataacca
    35281 cgattaacac ccaatgcagc aggcagtttt tatctttgga cagcgaattc agaagtattt
    35341 ttagcggact gcaactttta catttcagcg gaagctgact cgcaaattcc ccttgaaaat
    35401 actccggcag cgttagccac cggagaggaa attatttcag cgatccctta aggaactgtt
    35461 gcagacgagg gctttgcggg ttgccaaata actgctccgg cgcgccctct tcttctattt
    35521 tcccctgatg gaggaaaatg acgtgagtag aaacatggcg agcaaagccc atttcgtgag
    35581 tcactaccac catggttttc ccctcttctg ccagttgctg cataatacgt aacacttcgc
    35641 ctaccagttc aggatcgagc gccgaggtag gttcatcaaa cagtaaaact tctggttcca
    35701 tcgccagcgc ccgcgcgata gaaacacgct gttgctgacc gccggaaaga tgcaccggat
    35761 atttcccctg cgcacgttcg tctatcccga ctttcgccag atacttcacc gcccgctcgc
    35821 gcgcttcctg cttgctcagg cccaacacct gaatcggcgc ttccatgacg ttttccaaca
    35881 ccgtcatatg gctccagaga ttgaagtgct ggaataccat cgtcaggcgt gtgcgcagta
    35941 agcgcagttg atttttatcg gcgactttga gttgaccgtc tttgtcgcgc accagattga
    36001 tcgtctggcc attgaccacg atcgatcctt cactcggttt ttcgaggaag ttaatgcagc
    36061 gcagaaaggt acttttcccc gatcccgacg atccgatgat gcttattaca tctccggcat
    36121 tcgcttgcag tgagacccct ttcagcactt cgtgttcgcc atagcgtttg tgcaaatcga
    36181 taacgtttaa tttattctcg gacatcgtat tctcagtgcg ttgaagaagg tttcacatgc
    36241 tgcaaccagc gtttttccgc tctgcgaaag aggctgatca ggacataaga gatgattaaa
    36301 tagagcaccg cggcaatgcc gaaggcggta aaaggttgat acgtggcggc gttaatatcg
    36361 cgggctattt tcagcagatc cggcacagtg gccgtaaacg ccagcgcagt agagtgcagc
    36421 atcaggatca cttcgttgct gtatgccggt aacgcaatac gcagcgcaga aggcaaaata
    36481 atgcagcgat acattttaaa ggtcgagaag ccataggctc tggcggcttc aatttcccca
    36541 tgcggaaccg aacggattgc cccagcaaaa atctcggtag tgtaagcgca ggtgttaagc
    36601 gtcagcgcca gcacggtaca gttcaggcca ctgcggaaga aagcgttaag gaattcggtt
    36661 cccttaacaa tctcaagcgt gtacatgccg gaatagaaca ccagcaactg aacatacagc
    36721 ggcgtaccgc gaaaaatata ggtaaataac cagattggaa actggatgta tttattactg
    36781 gagacacgac caatcgccag aaacagcgcc aggactccgc ctatcactac cgacaaaata
    36841 agcagccaca gagtgatcgc cacaccagta aagcgataac cgtcggtcca cagcagcggt
    36901 ttccagtatt catgtaagat ttcgatcaca ggtcagccct cttcacaccc acggagtagc
    36961 ggcgctcaag gaacagcagc acaccattgg aaacggtggt gaaaaccagg taaatcacgc
    37021 cacagacgat ggcgaaatag aacggttccc aggtactttt gcctgccagc tgcgtggctt
    37081 tgaccacatc ttccaggccg agtaacgaaa ccagtgcggt agatttgagg atcacctgcc
    37141 agttgttgcc aatgcctggc agggcgtaac gcatcatcgc cggaaacatg atccgccgaa
    37201 acacttgccc gcgagtaaaa ccgaacgccg tcgccgcctc tatatgccct ttcggcactg
    37261 ccataaacgc gccacggaac gtttcggtaa aataagcacc gtagataaaa ccgagagtga
    37321 taataccagc gaccatcgga tcgatatcaa tctgcccgac gcccatcgcc tccgtcacca
    37381 tgtttagcgc aatctgcaaa ccatagaaaa tcagcagcat caacaccaag tccggcacgc
    37441 ctcgaatcag cgtggtgtac ccttcgaaaa tcagccccga aagccgattt tgcgagagct
    37501 taccgccagc gccaattaaa ccgatgatta cagcgagcac tacagagctg atagccagct
    37561 ccagcgtgac gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac atgctgcctg
    37621 tcctgtcgtg tgtggtgaat taccgcctct tgtctccctc cggttacccg aagggagcgg
    37681 gctttctttc accgatggcg attaaccacc ataaacatca aaatcgaagt actttttcgc
    37741 taatttctcg taagtaccgt cagcgcgcat ttcggcaaag gctttgttca gtgcctcgcg
    37801 cagttcgtta tcttctttac gcaggcccat gccggtccct acgccaaaca gtttttcatc
    37861 tttaacagac ggaccaccga atttgtaatc tttaccgacg ggttgtttga ggaaaccttc
    37921 gctggcagcg acctcatcct ggaacgcggc atcaatacgt ccggcagtca ggtcagaata
    37981 aatgttgtcc tgcccctgat acgagacgat ttcaatgcct tttggtgccc agtgttcatt
    38041 accgaacgtc tcctgggtgg tgccctgcaa tacgcctacc cgtttgcctt tcagcgactc
    38101 gactgtcggc tgaatgtcag aatttttcgc caccaccaga cgagaatcgg cagcgtacag
    38161 tttgtcggtg aaggcaattt cttgctgacg tttttccgta atggaaagcg atgacatgat
    38221 ggcgtcaatc ttcttcgctt ttaaggacgg gattaacgca tccagcgggt tttcgacaaa
    38281 cgtacattgc gtattgatgc gcttgcacaa ctccttcgcc agctcgatat cgaaaccaac
    38341 cagttcgcct tgtgaatttt ttgattcaaa tggcgcatag gtcgggtcgg taccgatgcg
    38401 gatgttttgc ggaattgcag caagtgccgc agttgcgctg gagaaggcca gaaccagaga
    38461 gagcgatagc accagttttt tcataatgtc ctcaactgac agtcttttta tagggaatat
    38521 ttacaggttt gttacactta tcgtgccata aaattgacca tcaaggcaaa atatcccgcc
    38581 ccgttagacg tattattttt cattaaaaat gcttaagtat gcatttcaat gcaccacgat
    38641 agtgcaacaa acctggcgtg caccatgatg atcattccat caggtacagc ttcccagcga
    38701 cgtatcagtc accgtagaca ttaaagtcga aatacttttt cgccatcttg tcgtaggtgc
    38761 cgtcctgacg cagctcgcca agcgccttat tgaaggcagc cgtcagttca gcataatctt
    38821 tacgtagccc tacaccggtg ccatcaccga agtatttttt gtcttttact gatgagccag
    38881 caaaagcgaa atctttacca gcaggttgct tgaggaatcc ttcgctggca gcaacttcat
    38941 cttgtaacgc agcatccaga cgtcctgcag ccagatcgga atagaccaaa tcctggttgg
    39001 cataggccac cacatccacg cctttactac gccaggtctc gttagcgtaa gcttcctggg
    39061 ttgatccctg cagcacacca acatgtttac ctttcagtga atccagcgtt ggctgaatcg
    39121 gtgaaccttt ggcagcaatc aaacgagaat ctgcggcgta cagcttgtcg gagaaggcaa
    39181 tctcctgctg acgtttatcg gtaatggaaa gcgacgaaat aatagcgtcg atttttttcg
    39241 ctttcagtga ggggatcagc gcgtcaaagt cactggcaac ccaggtacat ttcacctgca
    39301 tccgtttgca catctcgtta ccgagatcga tatcaaagcc aacaaaatca cctttagcat
    39361 ctttcgacga gaacagcgca taggtggtat cggttccgat acgtaccgtc tccggtagcg
    39421 ccgcatagct ggaagccgct gcggagagac cgactaataa agacagagcg agaatcgact
    39481 tcttcataca taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg tgttgtgtgt
    39541 ttgcaggctc tttcatgcag gtcttatgcc atcttgacga caacagtaac attcaacgtt
    39601 aaatatgtta ataagacatt gcattattgt cctgaagttg aagatagcag gtatagcggt
    39661 tgaatcgcag cgtttcgatt gaatggcaga aacaaaatgt cgaggatttg atcgcggttg
    39721 caaaattgcc ctgaaacagg gcaacagcgg agttatgcgc cctgccagcg ggcaaagaga
    39781 tcttcaggaa gggttattgc aaactggtca agaacacgat taaccgtctg atttatcaca
    39841 tcatcaaggg attgcgggcg atgataaaac gccggaacgg gaggcataat caccgcaccg
    39901 atttctgccg cctgagtcat taaacgcaga tggcctaagt gcaatggtgt ttcacgcacg
    39961 cagagcacca acgggcgacg ctctttcagc accacatctg ccgcacgggt cagtaagcca
    40021 tcagtatagc tatggacaat gccggacagg gttttgattg aacagggtaa aatcaccatc
    40081 cccagcgtct ggaaagaacc ggaagagatg ctggcggaaa tatcgcgcgc atcgagcgtg
    40141 acatcagcta atgcctgcac ttcgcgcagg gaaaaatccg tttcgaggga taaggtctgg
    40201 cgcgccgcct ggctcatcac cagatgcgtt tcgatatctg tgacatcgcg cagaacctgt
    40261 aataagcgca cgccataaat cgcgccgctg gcaccgctga tgcctacaat gagtcgtttc
    40321 ataaatagtt gccctgtcag acttgcaggc agactttgca gtatttcggc gggagttgca
    40381 agtcagggtg ccagaccggc accctcagcg aaggcatcat ccttcgttat gcatttcgag
    40441 attttccact tcgttctgac gttgcactgc tttggcgtca tcattacgta acgtatcgag
    40501 gaaatcgagg tagccctgat caacatcttt ggtgacgtag acgccgttga acaccgagca
    40561 ttcaaactgc tggatatccg gattttcagc gcgaacggcg tcgatcagat cgttcagatc
    40621 ctggaaaatc aacccgtcag caccgatgat ctggcgaatt tcatcaactt cgcgaccgtg
    40681 agcgatcagt tccgtggcgc tcggcatatc aataccataa acgttcggga agcgaatttc
    40741 cggtgccgca gaagcgaggt acactttctt cgctccggct tcgcgtgcca tctcgataat
    40801 ctgctcagaa gtggtgccac ggacgatgga gtcgtcgacc agcaggacgt ttttatcgcg
    40861 gaactcggcg cggttggcat tcagtttacg gcgcacggac ttacgacgca gctgctggcc
    40921 cggcatgata aaggtgcggc caacatagcg gtttttaacg aagccctggc ggtacggttt
    40981 gcccagaata cgagcaattt ccagcgcgat atcacacgag gtttctggga tcgggatcac
    41041 cacgtcgata tccagatctt cccattcgcg ggcaattttc tcgcccagtt tcgtgcccat
    41101 attcacacgc gcgctgtaaa cggaaatttt gtcgataaac gagtccgggc gggcaaagta
    41161 tacatactca aacaggcacg gattgctgac cggattgtca gcacattgac gggtaaacaa
    41221 ctgcccttct tcagtgatgt aaatcgcttc gcccggcgcg acgtcacgca ggaaatcaaa
    41281 gcccagcgta tcgagcgcta cgctttcgga agcgaccata tattctgtac ggttctcgtc
    41341 aatatcacgt tttcccagta ccagcggacg aatcccgttt ggatcgcgga aagcaaccat
    41401 accgtggccg ataatcatcg ccacacaggc atacgcgccg cggattaagc ggtttgtggc
    41461 agcaatggca gcgaaaatat tgtcggcttc cagcgggtag tggcggaagt tgtccagctc
    41521 gctggcgaag atattaagca gaatttccga gtcggaagtg gtgttgatgt ggcggcgttt
    41581 ttcttcaaac agttttttac gcaactcgtg agcgttggtc agattgccgt tgtgggcaag
    41641 cgtaatgcca tacggggagt taacgtaaaa cggctgcgct tcagaggcgc tggagctgcc
    41701 agccgtaggg taacgcacat gaccaatgcc catattgccc tgcaaacgct gcatatggcg
    41761 agcttcaaat acatcgctca ccagcccgtt cgctttacgc aaacggaagc agttattggc
    41821 atctatggtg atgatgccgg cggcatcctg accgcgatgc tgaagcaccg ttaaggcatc
    41881 ataaatcgac tggttaaccg gcataacacc ggcgataccg acaataccgc acatacgtct
    41941 ttttcctcgt taagccacat ctcagagcac ttacgctctg ggcaagaaac ttgacgagct
    42001 ttgcagataa tcaaaaaacc atctgatgat aaaactgaat tgcgggatca gctgtgattt
    42061 gctccagtct tcacttttcg acaccccggt aaaggagtcg agaaagaaga gaatggcggc
    42121 aacaatcaac acaccgcgca acgcaccgaa acagacgccc agcacccgat cggtgcctga
    42181 caaccccgtt ttctccacca actggcctat cacgaagttc acgatagcac caacgatcag
    42241 ggtagcgata aacagtaccg cgatggcaat cccatttcga accagttcgt cttcaaagcc
    42301 cgtaaaccag actgacaggt aagtgtagta atgactggca acaaagaaag cacaacccca
    42361 tgtcaccagc gataacgctt cacgaacaaa gccgtggatc aggctaacca gagaggaaaa
    42421 agcaatcacc gcgattatgg cgtaatcaat ccagaccata tgtgtcccac gattttacgc
    42481 cctgtcatcc tgttcggggc gcattctaac agaaaaagaa aacgtttgcg tagggatttc
    42541 cttcccgcgc atcaataaaa atggcgctga aaaaatattc aacgccatcg actttttatg
    42601 cctttgcggc atcgtgcaat gcgtgtcgga tgcggcgtaa acgccttatc cgacctacgg
    42661 ttctaacact gcccaagcct gataagacgc gccagcgtcg catcaggcaa gactgtatta
    42721 attcggcgta tagcccatta ccacgccact taagccagaa agttgcttca actcacccag
    42781 cgaacctttc agcttatctt tcgaggcatc cggcccaacc agaatacggg taattttacc
    42841 ctgcactggc gtggatggcg acgtataaac ccgataaccg gcaccgcgca gcttaccgac
    42901 aatctcattc actttatcgg catttttcag cgcacccagt tgcacaacat aggctttacc
    42961 cgttggcgct gctttttctt ccacgaccgg cttcggttct ggcttcggcg caggtggtac
    43021 ttcaaccttt ggtttaggcg gctccaccgg tttcggcttc ggtggggcga ccggtgcagg
    43081 ttccggttca aactcggtgt tattggctgc aatagtggcc ggatcgagcg acggtgctgc
    43141 ggcatcacct gcccgcacct cttccgctgc gccttccggc ggctgcgtcg gcaacgcctg
    43201 ggtggcggct ggcatcatat caggctcatc acgatcgccc gctttcggca ccagcgggat
    43261 agccgcgaac tcatcctgat aatgtttttt ctgcccgtcc agcagccctg gaagtacaat
    43321 cacccccagc gccaccagca cgatcgtgcc cactaaccga ttctgaaact tacttgccac
    43381 cgcttctcct cgcgtcaatc acttccatga catgtgcgac cgtgtggaaa gaaccacaca
    43441 ccagcacggt gtcttccgct ttagcgtccg ccattgcggc atcccatgcc tgcgcaacgc
    43501 tatcaaatga tttgccgtta cccaaatgct caagcagttg ttctgccgtg gcaccgcgcg
    43561 gcccttccag tggcgcacaa taccagtcat caaccacgct tttcaaccag gccagagttc
    43621 cggcaatatc tttatcatgt agcataccga taaccgccag cacgcgcccg tttttcggta
    43681 gcgctttcat acgcccggtg agatattccg ccgcatgtgg attatgcgcg acatcaaaaa
    43741 taacgcgtgg cgactcgctc acaatctgga aacgtcccgg caaaattgcg ctggcaatcc
    43801 cgtcgcgaat ggcattttca ctgacttcca gcccgctggc acgcagtgcc gccagcgccg
    43861 ttgcggcatt cggttgcggg acaagcggca acggcagatt ttccagcgtg ccgtgagcat
    43921 cgctaaacgc ccagtcatga tcggtgacgg aatagttcca ctcaacgccc cgacgttgta
    43981 acagtgcacc tttttcctgc gccacatcag caatggtaga aggcatttcc ggctcaccga
    44041 caattgccgg tttttcgctg cggaagatgc ctgctttctc gagaccaata ctttcgcgat
    44101 ctggacccag ccagtcggta tgatccagcg caatactggt tactaccgcg acatcggcat
    44161 cgacaatatt ggttgcgtcc agacgaccgc ccagccctac ttccagaatc accacgtcaa
    44221 gttgtgcctg cttgaacagc cacaacgccg acagcgtacc gtactcgaaa taggtcaggg
    44281 aaatatcacc gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc gattccggca
    44341 attcctggcc ctgcacacgt acgcgctcgg tataacgcac cagatgaggc gaactgtaga
    44401 cgcccacttt gtaccctgcc gccatcagaa tcgactccag cgtacggcag gtggtgcctt
    44461 tgccattcgt acccgcaacg gtaaacacaa atggcgctgg tttcaggacg ccaagacgcg
    44521 ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt tttactgtgc aggttttcca
    44581 gataagaaag ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg ataatcatgg
    44641 tatccgctga ttcgttacgg tgagaatagc aaaagggcag agccagtggc cctgccctta
    44701 tcagttatca ggcctcaggt tcctgatccg gtaccggggg taccactacg ccttcacgcg
    44761 gcgcttcagg attcggcgct ggcagattca tcaacttcgc cagaatgctc gccagtttca
    44821 ggcgcatttc cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc aggaattcac
    44881 tgcgctggaa tctaggcggc agtttttcgc gaacggtctg ttcgataaca cgcggaccgg
    44941 caaagccgat taacgcttta ggttcagcga tgttgagatc gcccagcatg gcgaaacttg
    45001 cagaaacacc gcccatcgtc gggtcggtca gcacggagat gtacggcaag ccgcgctcct
    45061 gcatttttgc cagtgccgca gaggttttcg ccatctgcat cagcgacatc agtgcttcct
    45121 gcatacgtgc gccaccagag gcggagaagc agatcagcgg gcagttatct tcctgcgcct
    45181 gctcaacggc acgcacgaaa cgtgcaccca caacagaccc cattgaaccg cccataaagg
    45241 cgaactcgaa tgccgcagcg acaaccggca ttccatacag agtgcctttc atcaccacca
    45301 gcgcatcttt ttcgccggtt tctttctgcg cagatgccag acggtcttta tacttcttgg
    45361 agtcacgaaa cttcagctca tctttcggct caagctcgct acccagctcc acaaggcttc
    45421 cttcatctaa caggctatgc aggcgattac gcgctgtcat acgcatgtga tggtcacact
    45481 tcggacagac ctcaagatta cgttccagct cagcgcggta taaaacctga ccgcagctat
    45541 cacacttagt ccacacccct tcaggaatgc tcgccttgcg ggtgggagta atgttgcttt
    45601 taattcgttc aatccagctc attagggacc tttctgtctg aacctggttc gatgccagtt
    45661 ttatctttgg ggacgcataa tgccattttt gcccccaaca gaccatgaat gttgcacatt
    45721 aaaacataac agcccgaaac tttggataaa aaagtggtcg aaccgcggag ttacttttta
    45781 ttttgcggcg cgtgccgcag cgcgtttgtg acggattatt tcgatgacgc ccggcaaaat
    45841 agaaaccaca ataatcccga cgatcagcag cttaaggtta tcctgaacca tcgggattgt
    45901 accgaagaaa tagcctgcgt aggtaaaaag cagtacccac aacagtgcgc cgatcacgtt
    45961 ataagcagcg aaatgacggt acgacatgtg gcccattccc gcaacaaacg gggcgaacgt
    46021 tctgacgatc ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt tctcataaaa
    46081 ctgatgggtt ttgtcgagat aactgcgacg gaaaattttc gaattgggat tactgaataa
    46141 cttttcaccg aacaaccgcc cgatagtgta attgaccgcg tcacccacaa tcgcggcgat
    46201 cagcatcagc actaccatca tatggacgtt gagatcgttg gtttccagtg atgccaacgc
    46261 tccggcaaca aacagcaatg aatcacccgg taaaaacggc attaccacca gaccggtttc
    46321 acagaacaaa attaagaaca aaatggcata aacccagacg ccgtactccg cgactagctc
    46381 tgccagatgc acgtcaatgt gcaggataaa atcgatgagg aaataaatca ggtccatatt
    46441 tgcctatgcc ttgtactcgt catctttcag gctgtaacta cgttggctac agcctgaaat
    46501 gttccgagca ttattctcgt tagtccgcca gaaatagcgg gcccattggc ggttttggaa
    46561 gatcataccg gtcagggtaa tccaccgcga ccagatacag cccttccgct tttgccgttg
    46621 ctgccgccag cgttctgtcc tttgccgcca gcaactctgc tatccagctc tccggctggt
    46681 tgtgggcacc gacttccatc aggctgccga caatattcct gaccatatga tgtacaaagg
    46741 cattcgcttt gatatcaacc accacataag gaccgtgacg cgtgacatta atatgcataa
    46801 cgttgcgcca cggggttcgg gactggcact gcaccgcacg gaacgaggtg aaatcattct
    46861 cgcccagcaa gcattgcgca gcccgatgca tccgttcagc gtccagcggt tcgtaaaaat
    46921 gggttacccc tttactcagt accgccgggc gcagccgatg attgtagatg atgtagcgat
    46981 aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc aggtacagct ttaacccaac
    47041 gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa ggtccacgcc gcgtctttgc
    47101 gctgcgcggt tgtttcgaaa tgcaccactt gcccggtacc gtgtacccct gcgtcagtac
    47161 gcccggcgca gaagacggtg atgggttcgt tcgccacctg ggagagcgcc ttttccagct
    47221 tctcctgcac actgcggact tcattctgcc gttgccagcc gtaatactta ctgccgtcgt
    47281 actcaatgcc cagcgcaatt ttataaactg gcagttgttg ctggtcggac attagtacag
    47341 atactcctgc accagtttct cggcgatttt tactgccatc agcgcgccgc caaagcgaac
    47401 gttatcggcc accgaccaga actggacttg ctccggcata ccgtagtcat tacgcacgca
    47461 gccaacagaa agatgcggcg tacccgaagc atcccctacc tgagtcggga attcgttctc
    47521 ttcagagagc acaatatctt cgccctgagc aaacgcatca cgcgcttctt ctgccgccag
    47581 cggacgcagt gcttcaaagt tgaccatctg ggcatgaccg tagaataccg gtgcctggac
    47641 gacgcttgcc gaaatcatca gcccttcgtc ctgcaggatt ttgcgtactt cgtcaacgat
    47701 acgacgttct tcacgcacgc taccttcgct atccggcaac aacggcagca tattgaacgc
    47761 cagttgacga ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca atttcgcact
    47821 ctgccccgct aacgcatcga ccgctttttt gccctgggcg gaggctgaaa tcaggctggt
    47881 aacactgata cgcgacaaac cgccctgatc gattaacggt ttcagtgccg ccagcagctg
    47941 gctggtcaga ctgtctggta cggcgatgac attccggttg cggtaatcgg tcagtacaaa
    48001 cgggtttact tccggcacca ccagcggtac gtcgggttcg agagcaaaca gtccgctgct
    48061 gtcgatcacc aggcaacctg agttggtcgc ttcttcaacc caggcagcgg tagcttcttt
    48121 gcctgcgaca aaaaatgcca gctgcgcctg cgtccagtcg aattcagcgg catcctgcac
    48181 ggtgattgtt ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac gtgccagtgc
    48241 ataaatttcc ccaaccggga actgacgttc agccagcgtt tcaagcaggg cttcgcccat
    48301 agcgccagtt gcgcccagga cggcaatgtt ccagccttca gacatggtgg tttactccag
    48361 aaatagcaaa gctccctgcc aatgttacag cagagagcat gaagaagaga ttaacgtgcc
    48421 ggatgatgaa cggcgttaaa acccagttta cacagcaatg atgccgcact ggcgtcatca
    48481 caaattacat acagagatga ccattcacgg cgctcaagat agtttttgcg cagtttatcg
    48541 aactcacccg gtatcccggc gactttacgc agcggtgcgt catcgcggcg cacatcatac
    48601 accaaatgca ccagcctttt cagcgtcggt tgatcgagca gaccatgcag cgtaatgcgg
    48661 ccaaactcag gcgcgggcag caacgtatcc agcgcaacgt gttgttcatg cccaataaac
    48721 ttgctatagg cttcaaacac ttgcgtcgtg ccacgcgctt taccctccag ggtatagcct
    48781 gcgatatgcg gcgtgccgat atccactttt gtcagcaatt ccacattaag ttccggctcg
    48841 ccttcccaga catccagcac cacgcttaac ttctggcctt cattcaggca ggtcagcagc
    48901 gcggtattat cgacgactgc gccacggcag gcgttaatca gaatcgctcc gggcttcagg
    48961 ctacggatca gtttttcatc cgccagatgt agcgttttgt acggaccatc tttaaagagt
    49021 ggcgtatgga aagtcagaat atccgcgcgc tggactaact catccagcga gcggaaatca
    49081 ccctcatccc cacggtcggc gcgaggcgga tcgcaaagta aggttttaat ccctaacgct
    49141 tccagtcgcg cctgcaatcg acgtccaacg ttaccaacgc ccacgatccc caccgtacgg
    49201 tcgtgcagtg aaaatccatc gcgttcggca agcatcagca gggaggagaa aacatattcc
    49261 accaccgcaa tcgcattaca gccaggtgca gcggaaaaac caattcccgc ctgcttcaac
    49321 catgcttcat cgacatggtc ggtccccgca gtggcagttc caacaaattt aatgggtttt
    49381 cctgccagca aagattcatt cactttcgtg accgaacgca ccatcagcgc atccgcgtct
    49441 gccagttgag cgacggggat tggacgcccg ggaaccgcga tcacctcacc caaacggcta
    49501 aataagtcgc gggcataagg catattttca tcaacaagga ttttcacgtt tgtgttacct
    49561 gtatgagacg agagttaacc ggacaagtat gccataatct cgcggccagg catacttgcg
    49621 aagatttcag gtataaggat acgtaatgat acaacctatt tccggccctc ctcctgggca
    49681 gccaccaggt cagggagata acctgccgtc tggcgcgggc aatcagcctt tatccagtca
    49741 gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc aacaacaaag
    49801 agcagaactg tgggcgggta tcaggcacga tattggtctg tcgggagatt caccgctgct
    49861 ttcgcgtcac ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg ccgcgcaaaa
    49921 aagccattct gcccgccagc ttttagcgca attaggggag tatttacgtc tggggaatca
    49981 tcgtcaggcg gtcacggatt atatccgtca taactttggt cagacgccgc tgaatcagct
    50041 ctcaccggag caattaaaaa ccattctcac cctgttgcag gaagggaaga tggttattcc
    50101 gcaaccacag cagcgcgagg cgaccgaccg tcctttatta ccggcggagc acaatgcgct
    50161 aaaacagctg gtgaccaaac ttacggcggc aacgggggaa cccagcaaac agatctggca
    50221 atcgatgctg gaactttccg gggtgaaaga tggcgagtta attccagcga aactgtttaa
    50281 ccatctggtg acctggctac aggcgcgtca gacgctaagc cagcaaaata cgccgacgct
    50341 ggaatcacta cagatggcgc taaaacaacc tttagatgcc agtgaactgg cggcgttatc
    50401 ggcatatatc cagcaaaaat atggcctttc tgcgcaatca tcgctttctt ctgcccaggc
    50461 cgaggatatt cttaatcagc tttatcaacg gcgggttaaa gggattgatc cgcgtgatat
    50521 gcaaccgctg cttaatcctt ttccaccgat gatggacacg ttgcaaaata tggcaacgcg
    50581 tcccgcgctg tggatactgt tagtcgcgat tatcctgatg ctggtctggc tggttcgtta
    50641 accccgacga aatgacagta tcgtgacaat aatacccagc accgcagaga tcgccccggc
    50701 aagaaatacc gaagagtaac caaacgtggt cgccagcatt cccgccagcg gcccggagac
    50761 gccgagggcg atatcctgaa acgcggcgta accgcccagt gcggtgccgc gaacttgtga
    50821 ggggacgcgt ttaaccacct ccacgcccag cgcaggaaag ataagcgaac atccggctcc
    50881 ggttaacgcc gcgcccgcta atgcgaccca tgcacctggg gcttgccaga gcagcaacaa
    50941 gcccaccgtt tctacaagca gagagacaat cgccactttc acgccgccaa aacggtccgg
    51001 catccagcca aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg taagagtaaa
    51061 gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc cgataaccgc
    51121 aaaaccaacg ccttgtagtg ccagccctaa ccctggtttc cagataagcc cgacaacgct
    51181 ccacagcgat ggacgttctc ccgccagggc cggtactttg cgcactgtgc cgttacaggc
    51241 ccacgccagt aagggtaata ccattgtggt gatcgccagt gcggcaaaac cgtaatggct
    51301 atgaatcaac aggccaagcg gcgcaccaac agcgagggca ccgtaaatcg ccattccgtt
    51361 ccatgacatc actttgccag agtgttttgg ccctacgatg cctaaccccc aggtcagagc
    51421 gcctgtcagt aactggcttt caccaaaccc aagaatcaaa cgcccgatga ccaacagggc
    51481 aaatttgaac ggtgcggaga caggcaaaat cgccgccagc agcaacgcgc cgccagccag
    51541 accacaagct aacatcccct gaagcgccga acgttttgcg ccatattgat cggccagtcg
    51601 cccggcgtaa ccacgcgtca gcaccgtagc cagaaactga atcccgacgg caatgccgac
    51661 catggtattg ccatagccca gatcatgatg aacaaacagc gggataaccg gcaacggcaa
    51721 ccctacggtc atgtaggtga gaaaaaccgc aaaagagatg cggaagagcg aaaaactggc
    51781 agaagatcgt gtttcggttt ggcttacagc agtcatgcat tactccagaa tgcagcgcaa
    51841 ggcgaggagt atccccgtct catctctctg gtttcagggt tacggtgcgt tggcaggatt
    51901 taacgcgtac gtcttttcag aaggaaatcg acaaagcggg aagtttgcct agaactggtg
    51961 gcgattgtca atgatgtgaa aaagggaacc atcaggttcc cttttgcgtt agtgccggag
    52021 gccgcattgg cgcgtaacgt cggatgcgac gctggcgcgt cttatccgac ctactccggc
    52081 ctactgcgaa ttaatctttt cagcttgcgc attaccagcg tggcgttggt gccgccgaag
    52141 ccgaagctgt tagacataac agtggtcagt tcgcgatcgg tcgtttcggt cacgatgttc
    52201 agacccgcag cctgctcgtc cagctcttca atgttgatgc tcggggcgat aaagccgtgt
    52261 tccagcatca gcagagagta gatagcttcc tgcacacccg cagcgcccag tgagtgaccg
    52321 gtcatggctt tggttgcaga aatcgccggg ctcttatcgc cgaacacttc acggatagct
    52381 gccagctctt tcacgtcgcc aaccggagtc gaagtaccgt gagagttcag gtaatcgatt
    52441 ggggtatcaa cgccatgcat cgccatctgc atacaacgta ctgcgccttc gccagacgga
    52501 gcaaccatgt ctgcaccatc agaggttgcg ccgtagccaa cgatttcagc atagatgtaa
    52561 gcaccacgcg ccagcgcgtg ttccagctct tcaaccacta ccataccgcc gccgccagcg
    52621 ataacgaaac cgtcacggtg agcgtcgtaa gtacgggagg ctttttccgg ggtgtcgttg
    52681 tatttagtag atagcgcacc catcgcgtcg aattcgcagg ccatttccca gcacagctct
    52741 tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc tactgcgtta
    52801 ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg agtagttaac gccatgaatt
    52861 ttaaacggcg tggcgagaca ggcagaaacg ccggatgcca tcgctttagt gaccacatac
    52921 gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt cagcgccgaa cacctggaaa
    52981 cgcggggaac cgccgccgga acctgcaatc aggccaacgc gcgggttatt ctggtaagct
    53041 tccggagaga ggcccgcatc agcgattgcc tgctccatag aaaggaatgc ataaatggat
    53101 gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc cagtggtatc cagttttatg
    53161 ttgccccaga cgtggctacg catgccggaa tccttcagct cctgagagaa agtgatccct
    53221 gaacgtcctt cacgcagaga tgccaggact tcctgctggt tattaccgat gctggaaaca
    53281 atgcccaggc cagtaatcac tgcacgtttc attcaatacc tctgtaagtc gcacatagag
    53341 taagtttcga atgcacaata gcgtacactt gtacgccgaa caagtccgat cagccattta
    53401 atagagatat ttgcgcagcc ttacacacat cgctaagatc gagccaccgc ctgtaagacg
    53461 agtaacttac gtgaaacact actccataca acctgccaac ctcgaattta atgctgaggg
    53521 tacacctgtt tcccgagatt ttgacgatgt ctatttttcc aacgataacg ggctggaaga
    53581 gacgcgttat gtttttctgg gaggtaacca attagaggta cgctttcctg agcatccaca
    53641 tcctctgttt gtgatagcag agagcggctt cggcaccgga ttaaacttcc tgacgctatg
    53701 gaaggcattt gatcagtttc gcgaagcgca tccgcaagcg caattacaac gcttacattt
    53761 cattagtttt gagaaatttc ccctcacccg tgcggattta gccttagcgc atcaacactg
    53821 gccggaactg gctccgtggg cagaacaact tcaggcgcag tggccaatgc ccttgcccgg
    53881 ttgccatcgt ttattgctcg atgaaagccg cgtgacgctg gatttatggt ttggcgatat
    53941 taacgaactg accagccaac tggacgattc gctaaatcaa aaagtagatg cctggtttct
    54001 ggacggcttt gcgccagcta aaaacccgga tatgtggacg caaaatctgt ttaacgccat
    54061 ggcaaggttg gcgcgtccag gcggcacgct ggcgacattt acgtctgccg gttttgtccg
    54121 ccgcggtttg caggaagccg gattcacgat gcaaaaacgt aagggctttg ggcgcaaacg
    54181 ggaaatgctt tgtggggtga tggaacagac attaccactc ccctgctcca cgccgtggtt
    54241 taatcgtacg ggcagcagca aacgggaagt agcgattatc ggcggtggta ttgccagcgc
    54301 gttgttgtcg ctggcgttat tacggcgcgg ctggcaggta gcgctttatt gcgcggatga
    54361 agcccccgcg ctgggtgctt ccggcaatcg ccagggggcg ctgtatccgt tattaagcaa
    54421 acacgatgag gcgctaaacc gctttttctc taatgcgttt acttttgctc gtcggtttta
    54481 cgaccaatta cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt taggctggga
    54541 tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg gatttacccg cagaactggc
    54601 tgtagccgtt gaggcaaatg cggttgaaca aattacgggc gttgcgacaa attgcagcgg
    54661 cattacttat ccgcaaggtg gttggctgtg cccagcagaa ctgacccgta atgtgctgga
    54721 actggcgcaa cagcagggtt tgcagattta ttatcaatat cagttacaga atttatcccg
    54781 taaggatgac tgttggttgt tgaattttgc aggagatcag caagcaacac acagcgtagt
    54841 ggtactggcg aacgggcatc aaatcagccg tttcagccaa acgtcgactc tcccggtgta
    54901 ttcggttgcc gggcaggtca gccatattcc gacaacgccg gaactggcag agctgaagca
    54961 ggtgctgtgc tatgacggtt atctcacgcc acaaaatccg gcgaatcaac atcattgtat
    55021 tggtgccagt tatcatcgcg gcagcgaaga tacggcgtac agtgaggacg atcagcagca
    55081 gaatcgccag cggttgattg attgtttccc gcaggcacag tgggcaaaag aggttgatgt
    55141 cagtgataaa gaggcgcgct gcggtgtgcg ttgtgccacc cgcgatcatc tgccaatggt
    55201 aggcaatgtt cccgattatg aggcaacact cgtggaatat gcgtcgttgg cggagcaaaa
    55261 agatgaggcg gtaagcgcgc cggtttttga cgatctcttt atgtttgcgg ctttaggttc
    55321 tcgcggtttg tgttctgccc cgctgtgtgc cgagattctg gcggcgcaga tgagcgacga
    55381 accgattccg atggatgcca gtacgctggc ggcgttaaac ccgaatcggt tatgggtgcg
    55441 gaaattgttg aagggtaaag cggttaaggc ggggtaatct gctctcgcag tgtttgccgg
    55501 atgcggcgtg aacgccttat ccaacctacg tgtgacgtgt aggcctgata agacgcgata
    55561 agcgtcgcat caggcatggt gctccagacg ccgcaactta cagctgtgac gcctgctgaa
    55621 acaggttttc ccacatatcg gtcaccagcg cctggtcacg cggcgacaat tcaccggcac
    55681 caatggcttt ttccagactc tggctaacgg tcgtatgcac cgcctgagcg gagtggtcat
    55741 caccactttc cagttctgcg atggctaatg tcaggtggcc acgcaaatac ccactggcaa
    55801 acaactcatc atcactggca tggtcaacca tgccgtcgat taatgccaga atgcgtgatt
    55861 caaactccgc gatcatcttc tttcctcatt gtaaaacgtg gcgctggctt cagttgagcg
    55921 cttccggcca cgggaactgt tccgccgtaa gttccggcgt gtgataataa ttttgtagtg
    55981 ctttaatgaa gcgtgccggg cgttccggaa tgccatttgt cagataatcc atcacctgcg
    56041 catgtacccg ccgttgaaac accacacgat ccggttcgaa atccccttcc agattgtcgc
    56101 aactgacgtt aaacggatat ccagccgcca cgcagaataa ccagtcgaac gcctgcggct
    56161 tcacttcaac atcttcaaac tggctttgcg tctgggcatc gcgtccgtcc gggcaatacc
    56221 agtagccgaa atcaaccagc tcacggcgcg cttttccggc aatacaccag tgcgaaatct
    56281 catgaattgc gctggcataa aagccatgag caaagacgat tcggttatac ggtacttccg
    56341 catcagcagg aagatagatc ggttcgtcgt cgcctttaat cagacgggta ttaaattcat
    56401 cggcaaagca gctattaaaa atttcaatca actgctcgta gtggtgtgta ctgttcatta
    56461 gttcatcccc aaccagtgga ggatctcctg tccgtggcta tcgtaaagta atttggcact
    56521 catcaccgcc gagacgataa caatcatcgg gcggatcagc ttttgtcctt tgctcaacac
    56581 caaccgtgaa cccatgcgcg cgccgaggaa ctgccccacc agcatgacaa agcccgtcgc
    56641 ccaaatcact ttgccgccga gaataaacag cagcagaccg ccaatgtttg acgttgcgtt
    56701 gagtaatttg gcgtgagccg tggctttggc gaggttgaat ccgcacagca taacgaaggc
    56761 cagcgcgtaa aacgatccgg cagccgggcc aaagaatccg tcataaaaac cgacgcagcc
    56821 accggcaatt aacgcgaagg gtaaaccgta catccggcgc tggcgatctt cttcacccag
    56881 ctttggcatc agcaagaaat agaggccgat acaaatcacc agaatgggca aaatctgccg
    56941 taagacatca gcctgaacgt actgtaccaa cagtgcgccg ctcattgagc cgacaaaggt
    57001 catggcaata ttgagtttct gatcgcttaa actaaccact ttgcggcgaa taaagtagat
    57061 agtggcggaa atagagccgc cgcaggcttg cagtttattg gttgccagcg cgttagcggg
    57121 agacattccc gcagccatca atgccggaat ggtgagtaac ccaccgccac cggcaatcga
    57181 gtcgataaat cccgccagca tggcgacaaa aaagagaact cccagcaaca gcggagaaac
    57241 cataaacagg ctattaaacg tttccattag atcacgtgct catccagtag cgcctggcag
    57301 gaaggcggca acggaggcgg tgtcttcttc tcaggctttg ttgttcccgg ttttggaggt
    57361 tcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg atggcggtaa aggttgatct
    57421 tcacactcca gactatcggc agggcaacgt aatcgtacat gcatatgcgc gcgatgctgg
    57481 aaccagggtc gcactttgcg caaccagtcg cgatcggtgc ccgcatcaag gcaaagttgt
    57541 tgtttaatcg ccggattaac aaaaatgcgc gtgacgtctt tgtcctgggc ggcgagtttg
    57601 atcaagctga aaatttctgg cttccacagc gtggagacaa cgtgtttacc gtcgcgggaa
    57661 actaagtcca gtgcttgcgg gcgcaagagc tgcgcggagg tccagcgagt tttcggcagt
    57721 tgcagaaaga tatcgacatc cagtccggtc tggtggctgg catgaccgcc gttgaaacgc
    57781 ccaccagcgg gcatccccat atcgccaatc agcaccgtac ccatgcccag attgctcacc
    57841 tggctactca gacgctggat aaacatcacc agatccgggt gaccgaaata gcgacgctga
    57901 tcggtacgca tgacctgata atgttcggac tgtatcggca acgtgtcagc gccgacaata
    57961 cagccattag aaaaactgcc tatcgattgt gcgctacccg gcacaggttg ggttattttt
    58021 tgccacggcg tcgctgccag gctagcacta ctggcaagca gagccagcag cgcaatcgcg
    58081 gttttgttca ttttttacca gcgtggaata tcagtcttca catcggcatt ttgcgcccgc
    58141 tgccgtaaca ggtgatccat taaaacgatc gccagcatcg cttcagcgat cggcactgca
    58201 cggatcccga cacagggatc gtgacggcct ttggtgatca tctcaacttc ttcgccaaag
    58261 cggttaatag tgcgccccgg cacggtaatg ctggaggtcg gtttcagcgc catatgagca
    58321 atgatttgct gcccgctgct gataccgccg aaaatgccgc ccgcatggtt gctctggaaa
    58381 ccgtctttgg tgatttcgtc gcggttctgg ctgccgcgca gcgccactac gtcaaaaccg
    58441 tcgccaattt ccacgccttt caccgcgttg atgctcatca gcgcatgggc gatgtcggca
    58501 tccaggcggt caaagaccgg ctcgccaagt ccggcgggga caccactggc aacaacggtg
    58561 actttcgcgc cgatggagtc gccctctttt ttcagcgcgc gcatcagttc atctaacgcg
    58621 tcgattttgt cggggtccgg gcagaagaac ggattttgtt caaccagcga ccagtctttg
    58681 atttccagcg gaatgtcgcc catctgggtc aggcagccgc ggatttcaat accaaatttc
    58741 tcggcgagat attttttggc aatcgcccct gccgccacgc gcatggcggt ttcgcgggcg
    58801 gaagaacgtc caccgccgcg ataatcgcgc agaccgtatt tttgttcgta ggtgtaatca
    58861 gcatggcctg gacggaagac gtctttaatt gcgctgtaat cctgagaacg ctggtcggta
    58921 ttttcgatca acaagccaat gctggtgcca gtagtaacgc cttcaaaaac accggagagt
    58981 attttgacct gatccggctc gcggcgctgg gtggtatagc gcgatgtccc agggcgacga
    59041 cgatcaaggt catgttgcag gtccgcttcc gtcagcggaa tgcctggcgg aacaccatcg
    59101 acgatgcagc cgagcgccag cccgtgcgat tcgccgaagg tggttacgcg aaagagttgt
    59161 ccaattgtgt ttccagccat cacggctccg ttattgttgt gtttgcgtgt ttacttaatc
    59221 tttataaatc gcgaaatgtt ctcgtgcggc aataagctgc tctttagtga gcataaacac
    59281 accatcgccg ccgttatcaa actccagcca ggtgaacgga acatcgggat attgttccat
    59341 aagatgtacc atgctgttgc ccacttcaca aatcaacacg ccatcatcag caaggtaatc
    59401 tgccgcgtta ccgagaatgc gacgcgtcag tttcaggccg tcggtgccag atgccaggcc
    59461 cagttccggc tcgtgacggt attcgtttgg caggtcggac atatcttccg cgtcgacata
    59521 cggcgggtta gtgacaatca gatcgtattg cactttcggc aaatcacgga acagatcgga
    59581 acgaatcgga atgacgttgt ggatcagacc gtgttcttcg atgttctgtt cggcaaccgc
    59641 cagcgcgtcg ggagagatat ctaccgcgtc gacttccgct tccgggaagg cataagcaca
    59701 ggcaatggcg atgcagccgc taccggtaca catatctaaa atatgctgcg gttgcttgct
    59761 gatgagtccg gcaaatttat tgttgatcag ttcaccaatc ggcgagcgcg gcaccagcac
    59821 gcgttcatcg acgtaaaatt catgaccgca gaaccaggct ttgttggtca ggtaagccac
    59881 cggaatgcgc tcgttgacgc ggcggatcac gcgttcaaca atacggtgtt tttcgctgga
    59941 ggtcagacgc gcggtgcgca tatcttccgg gatatccagc ggcaggtaga gcgatggtaa
    60001 caccagctgt acggcttcat cccacgggtt atcggtgccg tgaccgtacc agatatttgc
    60061 cgcgctgaag cggcttaccg accagcgcaa tatgtcctga atggtttgca gctcatttac
    60121 tgcttcatca acgaaaattt tatccacgta ttcctccagg gcatgatcgc aataatttcg
    60181 gcggctagtt tgccatgaag atgacgataa atcagcattc acgcgcggtg agtgaggaaa
    60241 aatacgttta aaacgatcga ttgtgctacg agtcgggtaa actgtaggaa aattagaaat
    60301 agagacagat aaatgaaaaa gaaaacaaca cttagcgagg aggaccaggc tctgtttcgc
    60361 cagttgatgg cggggactcg caagattaag caggacacga ttgtccaccg accgcagcgt
    60421 aaaaaaatca gcgaagtgcc ggtgaaacgc ttgatccagg agcaggctga tgccagccat
    60481 tatttctccg atgagtttca gccgttatta aataccgaag gtccggtgaa atatgttcgc
    60541 ccggatgtca gccattttga ggcgaagaaa ctgcgccgcg gcgattattc accggaattg
    60601 tttttggatt tacacggtct gacgcagctg caggccaagc aggaactggg cgcgttgatt
    60661 gccgcctgtc gccgcgaaca tgtgttttgt gcctgcgtga tgcatgggca cgggaagcat
    60721 attttgaagc aacaaacacc gctgtggctg gcacaacatc cgcatgtgat ggcatttcac
    60781 caggcaccga aagagtacgg cggtgatgcg gctttgttgg tgttgattga agtggaagag
    60841 tggttgccgc cggagttgcc ctgaatgctg gcagcaaatg ccggagcagc gtaaacgcct
    60901 tatccggcct acaaatatgt gataattcaa taaattgcaa ttcacttcgt aggcctgata
    60961 agacgcatca gagcaatact acgaacttca gttcattcaa tacatactga aaacgacacg
    61021 tcgaatcaat cggttgccca ctactggtat tcacattcac cacaatatcg tgtagtactt
    61081 tatccgttac cggatgacga gttggcgtaa ccgttaccgt tgatttatca caaccgtaag
    61141 aggtaaaggt taacaattct gataccgcgc ggcagtatgc tcctctgcca tcattatcag
    61201 aaaaattact gccatcacag agtctgccgc cttccatcag gtaggtcgaa agcgtggtgg
    61261 tcactgttga ccccgtggac tgaattcgta acgccttatt atggctgaca taattttcac
    61321 tccaggagat atcgtatgaa gcgaccggta tcgttgggca tattgtgtta tgcactgtag
    61381 gggccgtata gcttccaaat ccgggtaaat ctgtatagga ataaaaggat gcgatccaag
    61441 ggcaaccata atcatcagat gcaacggtaa ttttaaacgt catatatgca caacgaaaaa
    61501 ttttcgtatc tatgccaggg caatttgagt tattaattgt atcaaagtca gtcagtacca
    61561 aagttggagc tgaacccgac ccagaccagg aaataacccg caacgtatcg cctttatacg
    61621 caaaagaatc atatttccca tatgcaacct cctttggcgt tatcaccgta acattgaaaa
    61681 cactgctcgc tacagcaccg gttaccttgt ccatggcata ataaactctc acactgtcaa
    61741 aactcgcact cataagttta tctccagcat aactcttaga tgcgccgaca acactcagaa
    61801 cgagcagcat taggctcgcg aaaattatgg tccatttttt catttcagaa atactcaaag
    61861 gttgccgtca ctgtggcact aaagtctccg gacgttacat ctcgcccgct ctttgcctgt
    61921 aaccaggtat taaaatttag gctgttattt ccctgattca gcgggagcgt gacgcctgtt
    61981 gtattgttga ttggcaccga tgtgcctgcc gcagtttcaa taccaatccc agcgccggat
    62041 gctgatgacg aggagtcaaa ggccagaaag ccgggtagag cactatcttc tgttcctgtc
    62101 aacgttactt tgacattaaa caatgtagaa ctatgacaat ctttcaattg aaacaccaat
    62161 ggcactcgct ctgactgccc aaggttcatt aaatcactgg cggcaatggc aggaaaatgt
    62221 acttctgcca actggctacc attaatcact ggcgtacacg atttactgat taaattgccg
    62281 gtaaagtgca aattatctcc tgctattgtg gttccactgc ccagcaacat caggctacta
    62341 ataaaaaaca acgtacgctt tttcttcatt ggtaatctac ctgtaacgtg gcccaggcct
    62401 caaagtccgc ctctggcaga atgacaccac tcttttttac tggcacagcc gttaacaccg
    62461 gtaaatcggt ttcattcacc acaagtggcg tattgatcgt aaaactctgc cctgcctgtt
    62521 tgagctgaat gcctaaacca gtgatattgc tgcttactgc agcattatca aaatcgcttg
    62581 gtgtgccact ccagctcaag gtcatttcca ttaatgggtc gcgaaccgcc gtatcgcagg
    62641 taatggaata cggcacggtt tgctcataat tgctgccatt aatgtcatca ataagcacat
    62701 cctgaaaagt tatttcaatg gtttgagcgt tgctaatcgt gcagttaggc ggctgcacta
    62761 aataaccgtg aaaagagacg tttgagagtg ccatcgagca aaagccccac agcaaaataa
    62821 gcgagatccg tttcattggt aatccaccac aattgtggca ctggcgttaa attcagctgc
    62881 cgctaacgtt gtaccactct gtttcaccgg cacagcttcc agaacgggaa ctcccgagga
    62941 aaaattaaat ggcaaccatg agccagaggt taagtcgagt attgtgtgat cactactttt
    63001 ctgaacccga ataccaaacc caggaatacc cgtgctgata actgtttcac cgttaatcac
    63061 gaccgttgtc gcctgcatct gtagacgcag gtcatccatt gccagattgt tacaaacaag
    63121 cgagtagtca atgggacgtt tataatcaac cctattgatc ttattaatga atacattacc
    63181 aaactcaact gccgcaccat ttaccgtaca aggaggaggg gcatcgacaa gcacccgtaa
    63241 attcatactt actgacttac tcgctgcaac tgcaaagccg ctaccaacac aacatatcag
    63301 taacccagtt accgcccaga cagaacaaga tcctttcata ctggctctcc cggcctcagc
    63361 ctttgcggct ctgctcttca tcgacctagc aactgttacc gttgcaagtg aaaaagagcg
    63421 gcatccgagc gccatagtcg ttgacatagg tcagtaccgg cacgctaccc atcttcacat
    63481 tcagcggtgc agaggttttc ggcgacatca ctattggcga gaaaccggct gccggattgc
    63541 catttttctg tgtgctggca ttactgaaaa tgacgtaata cgacgtcggg ttattgacgg
    63601 taaattcctg accgttgcgg gttaaagtaa tcttatattg ctaggggttt ttcatgctga
    63661 cgttctccag cgccttcggt cgccagaaca acttaatacg cgtttgtagt gctatttgca
    63721 gcgtgttggc tttgtttgat tttggcggga tctcacgcac gttgaagtag aacaggcttt
    63781 cacgatcggc aggcaatttg ttgatatccg gcattccctg cacttttacc tgcccgttca
    63841 tcattgaatc gatgcgttgc accggtggca gaacagttaa cggtgaactg atcttgttac
    63901 ccttttcatc ttcgatccag ctttgggcca gataaggcag tttcggatcg ttattgcgca
    63961 acgtcacgct gattgatttg tcgctttcat taaatatcaa acgggtacga tctggcgtta
    64021 cagaagcctg ggcagacaaa ctggcagcgc taagcagtaa cgctagcgtc gtagcaccca
    64081 atttggcaga gcaaagtaaa tcagacatgg atcattcctt aatcacgtta ttggttagct
    64141 gaaacagaaa cagggggatt ggtgggtgtc acctgttgcg tctgctcctg aatgacaggc
    64201 tttatgtcat caggaacaac cggcgcgacg ttgcctttat gctggcatgg cagtaacaag
    64261 ccgttaaaca gatcggcagg cagcgggtcc ggcaggttga tatcgcaatg cgcaacacca
    64321 ctccagaaca cactcatgtg ttcgccaggt ttcactccag ccagataaac attgccgtca
    64381 tcatcgacaa ggcccactgt ctgctcgtta tcatttttta cttctgcgcc aaacggagga
    64441 tggctgccgt cttgtaaacg cagcaccgcc atcgcttttt gaccactgat gacggcaaat
    64501 ttgcggtagc caatcgcccc ttcagttagc gtggcttgta ccaccgactg ggttgcttcg
    64561 gcgttttccg gcagtttgtt gaggtcgata tacgcctgat tgcggtaata gttattgacg
    64621 tcagaaacga cggctttacc aaacatattg gtataaacag cagcaccgtt accttctacc
    64681 ggaacatcgg caacgccatc ggcatcaatc aacaagcgtg tcccgcccat attctgggta
    64741 cggtgaagtg cgccaccgtg ggcagtaagc gttgcgccgc cctgtaacga caagcccgca
    64801 gaggtgtatt gcccttcatg gtagttcgca ctgaggtcaa cctgcgccag cgaaccatca
    64861 tggctgtaat agccgtcaac gctggtgtgt ttgtcactgg tgccgacgtt caactgatag
    64921 tgagtcgcgt catcgacacg gctgaaataa ccgacctgac tgctgtccgt cccactgcca
    64981 tagtaccgtt atagctaacg gtactgttgt cgccccacgg catactgagc gaaatgtaca
    65041 tgcctttgtc ggcctggttg tcatactcgt aacggtagcc agtcatcgag atgctgacat
    65101 tacgaatact gcccatattg aaatagtgcg agagcatgat gttgtagttt gtctgctcct
    65161 cgcgatccca gtaggtatgg cgggtatagt tgagataaac cgaaacaccc gcatcgcgga
    65221 agttctggtt ataagtggcg gtgtacatct ctttgtcgtt gcccgtgcgt accattccgc
    65281 tgtcactggc atccagatac tcgctcatgg tcataaagtt ctcttccgag aagcgatatc
    65341 cagcaaaagt aacgcggctg ttgagctggt caaagtcttt ggaataactc acacggaagg
    65401 agttaccgtc cagcgaacct ttgccataag cggtgtcttt atccagtttg gtgtgcgagt
    65461 gagtaacatc aaacgcgacc gcgccgaatg tagacaaatc gcgaccgaca ccaagcgccg
    65521 cagactgata gttttcatct cccagtgcgc cgccatataa cgaccagccg ttagcgatcc
    65581 cccaggaagc ttcagcacca gaaaaaaatt caccctcgac atggtgtccc cactcttgcg
    65641 gacggcccat catgatctta tagcgaacct gacctggacg agtgaggtat ggcatcgagg
    65701 cggtgctgat gtcatattcc tgcacctggc cgttctgttc ttcaatgcga atatgcaacg
    65761 taccggagac agaatcacca agatcctgaa tacgaaacgg tccagccgga acctgcgttt
    65821 cgtaaatcac acgccccatc tggctgacgg tcacttttgc tgtggtgtgt gccacgccgg
    65881 aaatgtctgg cgcgtagccg cgcagattgg gaggcaacat ttgatcgtca gtactgacac
    65941 tgccaccaac atagttaaaa ccatcaaaaa tatcggatct gaggtaatcc tcgcccagcg
    66001 ccagtttggc ttttaatgat ggtaacgccc gcctggcata gtagcgactc cactcccatt
    66061 tttttgagtt tcatcgccgc tgaattcatc gtcatcatta ctgcgagtat gttgatagtt
    66121 agtctgccag tcagcacgca tacgccacgg ccctaggtta accccaaccg tcccgttgcc
    66181 gctgatctcg ttactatcat caccgccatt ttcttcgtgc cgggtttgtg cgttgatgct
    66241 gtagtccgcg acgatcccgg agatgccgtc atcccaacgt gaaggcggat ccctgtcggg
    66301 ataagtatat tcgaggtaag cctgtggcag tgaaatgact aatgcggact ggcttaaatc
    66361 agccttaatt tccatgcctt ccagttgacc ggatttcagg catttagcat cgtggctcca
    66421 ttgcagattt ttcgccacgt cttcttttaa accaaactgc gctaccagtt ccggtgtcag
    66481 acaagcatag cttttgctcg cgtcatcttc accagcatac cagtaaatat cgtactcttc
    66541 cgccaatggc tgtttattta gttgaacctg taaattgtat tttccgggct caacatatcc
    66601 ctggctggaa aaacgcttca tatcaatttt tgtgtcgcct tttaattcca gaaaacggga
    66661 atcaaactga atgtcgtctt cagcccagac actgctataa ctccctgaca ttgccagcgc
    66721 aatgcaccac ggaagaatcc gtaatcgaaa aagagaatgg tcaggcatac acgtttctat
    66781 ccgtttttat tcttgttcgg tcaaaataca tttaaatccc ccatagcagt gtcgctatgg
    66841 gggtatgcct tacaggtaag taatctggaa ggtggtattt gcttcaaaat tacccagatc
    66901 tggcgcatca gctgcgccga ccagccaggc gttaaagttc agtgtctgct tcgctttgcc
    66961 tttgttggta gaagtgtctt tcactatttt ctggtcaata cccatgccgc ttttgtaaga
    67021 ggtacccaga gagtcaccga tcgccaggct aacattgtta aatgccgcac cggtatcggt
    67081 attgaaaatg gtgtaataat tgccgctatt tgcagaagaa acggtaccag taaaggtagt
    67141 ggtcattgtt tcctgagtat caaatacgca atcttgcaga cgaatctgga aacttttcgg
    67201 cgtggtggtg ccgttatttt tcagtttttc ggcaccgata tcacccatat caacttccag
    67261 tttgtgatcg tccggaacga tagaacacac tgagtcttca atggtgccgt aaaaacgtgc
    67321 agtaccgttg tt
//
LOCUS       DAJTUI010000008        67020 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTUI010000008 DAJTUI010000000
VERSION     DAJTUI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.008"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(62..1066)
                     /gene="cra"
                     /locus_tag="OO464_000585"
     CDS             complement(62..1066)
                     /gene="cra"
                     /locus_tag="OO464_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6225848.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1148..1234)
                     /locus_tag="OO464_000586"
     CDS             complement(1148..1234)
                     /locus_tag="OO464_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6225849.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1246..1737)
                     /gene="ilvN"
                     /locus_tag="OO464_000587"
     CDS             complement(1246..1737)
                     /gene="ilvN"
                     /locus_tag="OO464_000587"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6225850.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1740..3464)
                     /gene="ilvI"
                     /locus_tag="OO464_000588"
     CDS             complement(1740..3464)
                     /gene="ilvI"
                     /locus_tag="OO464_000588"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR6225851.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3583..3627
                     /locus_tag="OO464_000589"
     CDS             3583..3627
                     /locus_tag="OO464_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225852.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3782..4726)
                     /gene="leuO"
                     /locus_tag="OO464_000590"
     CDS             complement(3782..4726)
                     /gene="leuO"
                     /locus_tag="OO464_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6225853.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5385..5471
                     /gene="leuL"
                     /locus_tag="OO464_000591"
     CDS             5385..5471
                     /gene="leuL"
                     /locus_tag="OO464_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6225854.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5564..7135
                     /gene="leuA"
                     /locus_tag="OO464_000592"
     CDS             5564..7135
                     /gene="leuA"
                     /locus_tag="OO464_000592"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6225855.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7135..8226
                     /gene="leuB"
                     /locus_tag="OO464_000593"
     CDS             7135..8226
                     /gene="leuB"
                     /locus_tag="OO464_000593"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6225856.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8229..9629
                     /gene="leuC"
                     /locus_tag="OO464_000594"
     CDS             8229..9629
                     /gene="leuC"
                     /locus_tag="OO464_000594"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6225857.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9640..10245
                     /gene="leuD"
                     /locus_tag="OO464_000595"
     CDS             9640..10245
                     /gene="leuD"
                     /locus_tag="OO464_000595"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6225858.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10294..11472)
                     /gene="setA"
                     /locus_tag="OO464_000596"
     CDS             complement(10294..11472)
                     /gene="setA"
                     /locus_tag="OO464_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR6225859.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11574..11705)
                     /gene="sgrT"
                     /locus_tag="OO464_000597"
     CDS             complement(11574..11705)
                     /gene="sgrT"
                     /locus_tag="OO464_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR6225860.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11794..13449
                     /gene="sgrR"
                     /locus_tag="OO464_000598"
     CDS             11794..13449
                     /gene="sgrR"
                     /locus_tag="OO464_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6225861.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13483..13582
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13613..14596
                     /gene="thiB"
                     /locus_tag="OO464_000599"
     CDS             13613..14596
                     /gene="thiB"
                     /locus_tag="OO464_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6225862.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14572..16182
                     /gene="thiP"
                     /locus_tag="OO464_000600"
     CDS             14572..16182
                     /gene="thiP"
                     /locus_tag="OO464_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6225863.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16166..16864
                     /gene="thiQ"
                     /locus_tag="OO464_000601"
     CDS             16166..16864
                     /gene="thiQ"
                     /locus_tag="OO464_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6225864.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16978..17742)
                     /locus_tag="OO464_000602"
     CDS             complement(16978..17742)
                     /locus_tag="OO464_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6225865.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17828..18706)
                     /gene="araC"
                     /locus_tag="OO464_000603"
     CDS             complement(17828..18706)
                     /gene="araC"
                     /locus_tag="OO464_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6225866.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19045..20745
                     /gene="araB"
                     /locus_tag="OO464_000604"
     CDS             19045..20745
                     /gene="araB"
                     /locus_tag="OO464_000604"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR6225867.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20756..22258
                     /gene="araA"
                     /locus_tag="OO464_000605"
     CDS             20756..22258
                     /gene="araA"
                     /locus_tag="OO464_000605"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR6225868.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22458..23153
                     /gene="araD"
                     /locus_tag="OO464_000606"
     CDS             22458..23153
                     /gene="araD"
                     /locus_tag="OO464_000606"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6225869.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23228..25579
                     /gene="polB"
                     /locus_tag="OO464_000607"
     CDS             23228..25579
                     /gene="polB"
                     /locus_tag="OO464_000607"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6225870.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25744..28650
                     /gene="rapA"
                     /locus_tag="OO464_000608"
     CDS             25744..28650
                     /gene="rapA"
                     /locus_tag="OO464_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6225871.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28662..29321
                     /gene="rluA"
                     /locus_tag="OO464_000609"
     CDS             28662..29321
                     /gene="rluA"
                     /locus_tag="OO464_000609"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6225872.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29438..30253)
                     /gene="djlA"
                     /locus_tag="OO464_000610"
     CDS             complement(29438..30253)
                     /gene="djlA"
                     /locus_tag="OO464_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6225873.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30508..32862
                     /gene="lptD"
                     /locus_tag="OO464_000611"
     CDS             30508..32862
                     /gene="lptD"
                     /locus_tag="OO464_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6225874.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32915..34201
                     /gene="surA"
                     /locus_tag="OO464_000612"
     CDS             32915..34201
                     /gene="surA"
                     /locus_tag="OO464_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6225875.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34201..35190
                     /gene="pdxA"
                     /locus_tag="OO464_000613"
     CDS             34201..35190
                     /gene="pdxA"
                     /locus_tag="OO464_000613"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6225876.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35187..36008
                     /gene="rsmA"
                     /locus_tag="OO464_000614"
     CDS             35187..36008
                     /gene="rsmA"
                     /locus_tag="OO464_000614"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6225877.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36011..36388
                     /gene="apaG"
                     /locus_tag="OO464_000615"
     CDS             36011..36388
                     /gene="apaG"
                     /locus_tag="OO464_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6225878.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36395..37237
                     /gene="apaH"
                     /locus_tag="OO464_000616"
     CDS             36395..37237
                     /gene="apaH"
                     /locus_tag="OO464_000616"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6225879.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37315..37794)
                     /gene="folA"
                     /locus_tag="OO464_000617"
     CDS             complement(37315..37794)
                     /gene="folA"
                     /locus_tag="OO464_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6225880.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37985..39847)
                     /gene="kefC"
                     /locus_tag="OO464_000618"
     CDS             complement(37985..39847)
                     /gene="kefC"
                     /locus_tag="OO464_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6225881.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39840..40370)
                     /gene="kefF"
                     /locus_tag="OO464_000619"
     CDS             complement(39840..40370)
                     /gene="kefF"
                     /locus_tag="OO464_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6225882.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40418..41812)
                     /locus_tag="OO464_000620"
     CDS             complement(40418..41812)
                     /locus_tag="OO464_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6225883.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41871..42158)
                     /gene="fixX"
                     /locus_tag="OO464_000621"
     CDS             complement(41871..42158)
                     /gene="fixX"
                     /locus_tag="OO464_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR6225884.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42155..43441)
                     /locus_tag="OO464_000622"
     CDS             complement(42155..43441)
                     /locus_tag="OO464_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6225885.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43492..44433)
                     /locus_tag="OO464_000623"
                     /pseudo
     CDS             complement(43492..44433)
                     /locus_tag="OO464_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44448..45218)
                     /locus_tag="OO464_000624"
     CDS             complement(44448..45218)
                     /locus_tag="OO464_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR6225886.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45574..47088
                     /gene="caiT"
                     /locus_tag="OO464_000625"
     CDS             45574..47088
                     /gene="caiT"
                     /locus_tag="OO464_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR6225887.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47119..48261
                     /gene="caiA"
                     /locus_tag="OO464_000626"
     CDS             47119..48261
                     /gene="caiA"
                     /locus_tag="OO464_000626"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR6225888.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48390..49607
                     /gene="caiB"
                     /locus_tag="OO464_000627"
     CDS             48390..49607
                     /gene="caiB"
                     /locus_tag="OO464_000627"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR6225889.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49681..51234
                     /gene="caiC"
                     /locus_tag="OO464_000628"
     CDS             49681..51234
                     /gene="caiC"
                     /locus_tag="OO464_000628"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR6225890.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51343..52128
                     /gene="caiD"
                     /locus_tag="OO464_000629"
     CDS             51343..52128
                     /gene="caiD"
                     /locus_tag="OO464_000629"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR6225891.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52134..52724
                     /gene="caiE"
                     /locus_tag="OO464_000630"
     CDS             52134..52724
                     /gene="caiE"
                     /locus_tag="OO464_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR6225892.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52810..53205)
                     /gene="caiF"
                     /locus_tag="OO464_000631"
     CDS             complement(52810..53205)
                     /gene="caiF"
                     /locus_tag="OO464_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR6225893.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53467..56688)
                     /gene="carB"
                     /locus_tag="OO464_000632"
     CDS             complement(53467..56688)
                     /gene="carB"
                     /locus_tag="OO464_000632"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6225894.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56706..57854)
                     /gene="carA"
                     /locus_tag="OO464_000633"
     CDS             complement(56706..57854)
                     /gene="carA"
                     /locus_tag="OO464_000633"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6225895.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58310..59131)
                     /gene="dapB"
                     /locus_tag="OO464_000634"
     CDS             complement(58310..59131)
                     /gene="dapB"
                     /locus_tag="OO464_000634"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6225896.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59298..60212)
                     /gene="rihC"
                     /locus_tag="OO464_000635"
     CDS             complement(59298..60212)
                     /gene="rihC"
                     /locus_tag="OO464_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6225897.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60316..61266)
                     /gene="ispH"
                     /locus_tag="OO464_000636"
                     /gene_synonym="lytB"
     CDS             complement(60316..61266)
                     /gene="ispH"
                     /locus_tag="OO464_000636"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6225898.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61268..61717)
                     /gene="fkpB"
                     /locus_tag="OO464_000637"
     CDS             complement(61268..61717)
                     /gene="fkpB"
                     /locus_tag="OO464_000637"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6225899.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61804..62298)
                     /gene="lspA"
                     /locus_tag="OO464_000638"
     CDS             complement(61804..62298)
                     /gene="lspA"
                     /locus_tag="OO464_000638"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6225900.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62298..65114)
                     /gene="ileS"
                     /locus_tag="OO464_000639"
     CDS             complement(62298..65114)
                     /gene="ileS"
                     /locus_tag="OO464_000639"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR6225901.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65157..66098)
                     /gene="ribF"
                     /locus_tag="OO464_000640"
     CDS             complement(65157..66098)
                     /gene="ribF"
                     /locus_tag="OO464_000640"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR6225902.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66106..66324)
                     /locus_tag="OO464_000641"
     CDS             complement(66106..66324)
                     /locus_tag="OO464_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR6225903.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66427..66690
                     /gene="rpsT"
                     /locus_tag="OO464_000642"
     CDS             66427..66690
                     /gene="rpsT"
                     /locus_tag="OO464_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR6225904.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16259 a        18246 c        17252 g        15263 t
ORIGIN      
        1 tccagtcccg tactctacgc gccagaggga aattcacctg gcgcgtattt ttgttcgcgg
       61 attagctacg gctgagcacg ccgcggcgat agagattacg tttaatgcgc gttaaaccag
      121 gttttggctt acgcggttcg tccaggcttg ccaggacaat ctccagcaca cgctctgcga
      181 catcgcggtg acgttgagcc actgccagca ccggacactg taagaagtcg agcagttcgt
      241 tatcgccaaa ggtggcaatt gccaggtcag aaggcagttt gccgtcgcga cgcagcgtga
      301 catccatcac tccttgcaac aacgcaaacg acgttgtgaa cagcgcctgc ggcatcggat
      361 gcgtttccag ccatttttcg aataactggg cagccgcctc ccgctcatag ctgttggcat
      421 acaggaaatg cacttcgcgc ggatcatctt tccaagcagt acggaaacct tgttcacgca
      481 ggaagctgac agaaagctcc ggcagcgcgc caagataaag caccgtctcg gcgggaaact
      541 tacgtaactc ttccgccagc atttcggcat catcctgatc ggcaccaacc acgctggtga
      601 aatgttcacg atcgagggcg cggtccagcg cgacaatcgg gaacgggtcg ttagcccagc
      661 gttgataaaa aggatgctca ggtggcaacg acgtcgaaac aataatggca tcaacctgac
      721 gctgtaaaag gtgctcaatg cagcgcattt cgttgtctgg ctgatcttct gagcaggcaa
      781 tcagcagttg ataaccccgt tgccgcgcct ggcgttcaag atagttagcg atgcgggtat
      841 agctggtgtt ctccagatcg gggatcacaa gaccaataga acgtgtgcgt ccagcacgaa
      901 gcccagctgc cacggcgttc gggtggtaat tgtgctcacg caccacagcc atgacttttt
      961 caacggtttt gtcgctcaca cggtattgct tcgctttgcc gttaataaca tagcttgcag
     1021 tggtccgcga cactcccgcc agccgagcga tttcatccag tttcacaatt gccccttgcg
     1081 taaaatgtaa aaaccatagc cattgtactg gtatgggtta aattcattaa catctaagcg
     1141 cagaaaatta actgcggcaa ctgcttttat tccaggttcc cactgatttc gcaaaaaaag
     1201 cccaacgtga catgttgggc tgcaaattgc gcactgagat cattctcaac gcattatttt
     1261 atcgccgcgc gaaagtccga ccacaccaga gcgagcaact tccacaattt tcgccacatc
     1321 gcgaatcgat gctaaaaatg catcaagctt atcgctggtg cctgctaatt gaacggtata
     1381 aagcgagggg gtgacatcga taatttgccc acggaatatt tccgtattac gtttcacttc
     1441 gtcacgcccg taaccgctgg cctgaatttt caccagcatg atttcccgct caacatgcgc
     1501 gccctgcccc aactcactca cgcgcaagac atcgaccagc ttgtgtaatt gcttttcgat
     1561 ctgctcaagt actttttcat cgcccacggt ctggatggtc atacgcgata atgtcggatc
     1621 gtcggttggc gcaacggtca ggctttcaat gttgtagcct cgctgggaaa aaaggccaat
     1681 cacgcgggat aacgcgcctg attcattttc gagtaagact gataatatcc ggcgcataat
     1741 caggttctct ccgttttgct taaccacatt tcatccattc cgcccccgcg aatctgcatc
     1801 gggtagacgt gctcactgcc atcgacggta acatcaacaa acaccaggcg attattgcgc
     1861 acctgttcca gcgcctcgct aagtttgctt tccagctcat gcggatgaga gatctggatc
     1921 ccgacgtgac cataggcttc cgccagacgg acgaaatcgg gtagtgattg catataagat
     1981 tgtgaatgac ggccggaata gatcatgtcc tgccactgtt tcaccatccc cagatagcgg
     2041 ttattgagat tcaccaccag tacgggcaac tcgtattgca acgcggtaga cagttcctgg
     2101 atgttcatct gaatactgcc gtcgccagtg acgcaaacca cggtttcttc tggcaacgcc
     2161 attttgacgc ccagtgccgc aggtaaacca aaacccatcg tgccgagacc gccggaattg
     2221 atccagcgac gcggtttgtc gaatggataa taaagcgcag caaacatctg gtgctgcccg
     2281 acatcggacg tcacgtaagc gtccccatta gtcaaccgcc aaagagtctc gatcaccgcc
     2341 tgcggtttaa tcttttcact gtgagtgtca tatttcaggc actgaagagc gcgccactgt
     2401 tcaatctgct gccaccagtc gcggatctca tccagcggtt gatgggcgga ttcttgcgac
     2461 aggagttcaa gcatttgttc gaggacctgg cgagcatccc ccacaatcgg gatatccgca
     2521 gtcacggttt tagaaatgga agtaggatca atatcgatat gcagaacagt ggcatttggg
     2581 cagtactttg ccagattgtt cgtcgttcgg tcatcaaatc gtaccccgac ggcgaaaatc
     2641 acatccgcgt tatgcatcgt catattggct tcgtaggtac cgtgcattcc cagcatgccc
     2701 agtgcctgac gatgcgttgc cggaaacgcc cccattccca tcaatgagga aacaacgggc
     2761 agattcaacg cctccaccgt ttctttcaac tgctgatggc agcccgccat gattgcccca
     2821 ccgcctacgt agacaaccgg tttttttgcc gctaccagcg tttgcagagc acgcttaatt
     2881 tgccctttat gtccggtagt agtgggattg taagaacgca tactgaccga ctcagggcag
     2941 acatagggta atttgttcgc cggattaaga atatctttcg gtaaatcaac gaccactggt
     3001 cctgggcgac cacttgccgc cagccagaaa gcctttttca gcacctgcgg aatgtcttcc
     3061 gtttgcttaa ccagaaaact gtgtttaacc accggtcgcg aaatccccac catgtcgcac
     3121 tcctgaaagg catcgtaacc tatcaacgag gtcgctacct gcccggaaag gacaactaat
     3181 ggaatggaat ccatataagc ggtggcgatg ccggtaatcg cattggtcgc ccttggaccc
     3241 gacgttacca gcacgacgcc gacttccccg gtcgcacgcg ccaggccatc ggccatatgc
     3301 accgccgcct gctcatgacg aactaataca tgatcaatac cacccacggt atgcaatgca
     3361 tcgtaaatat caaggactgc gcctccggga taaccgaata cttgtttaac gccctgatcg
     3421 ataagcgatc ggacgaccat ctcggctcca gacaacatct ccatggcctg cctcactgtt
     3481 tgacggaaaa aatgtgtaaa acacattaac cgtccgatct tgcttaagca attggcagcc
     3541 agagacataa acgcagggta ataagaataa acaggaggaa aggtgaaaag aaacgataaa
     3601 atcctccatt acgaaggatt aaattgataa taaatcggct gaatcccaca acttacagca
     3661 atttctctat ttttttaatt aatcctaaaa tagagagtcc acacattcag aataaaattc
     3721 tacattcaat aaaaaatgca gaataaacca gacattcatg tctggcctat tctgcattca
     3781 gttagcgttt gcaaattgag actaattgtt cttccatcca ctgatggcct ttatcgcgcc
     3841 cggcagcttc atgccaagag agataacagg ttctgctgtt ttgttttaac ggcagcggta
     3901 atacctgtaa ttctaaggat tcagcgaact cttcagccag ccaacgcggc gcaatagcga
     3961 ccaaatgcgt ttgcgacacc acgctaagta cgctcatcat tgccatgccc tgatacgcga
     4021 tactggcttg cttatctacc gtgtcatacc aaggttgact aaatgacgcg aaacgatcga
     4081 gcgaaaccgc cgcatgttgt tcgttataaa catcatgttt cagtaacggg cctttaattg
     4141 ttggatgatt tttgctggct accagcacca tttcatcttt aaataatggc acgctggtaa
     4201 attcaggacg atggaagtct tcataactaa tcacaaactc cgtttcctga taacgcagct
     4261 gatgttcagt gttctgattt aatgaagact tgaacataac atgtatattt ggcgcaatct
     4321 gctcaatgtg attataaatc tgcgaggtca gaatgctgtc taacgggctg caaacacaaa
     4381 gatgaaatac acgttcactg ctcgcgggtt caaaacctga accaggcaat tcattttgta
     4441 ctagttgcaa tgcctggcga actgaaccaa aaagttgaaa tgcgcgagca gtcggttgaa
     4501 taccacggcc ataacgaaca aaaagctcgt cattaaacat caccttcagg cgtgcaacag
     4561 cgttactgac cgcaggttgc gacataccca gaacatgagc ggcacgggta atgttttgct
     4621 cctgcatcac ggcatcgaaa acggttaata agttgagatc gaccatgcgt agctgtggct
     4681 tgcttaactc cgccgtctct gaatgatctg tttgtacctc tggcatactt aactccactg
     4741 tcacacttaa ctccctttcc cttattggaa tgcagagaaa taatcctgaa gaatatgatt
     4801 tatcatgcat ataaaataag aaaaagcaaa atgagtaaaa ttcggaaaac ataaagatac
     4861 gggttctgat aaaaacagaa tccattgccg tggaatgagt catttaccac agcataataa
     4921 tccataatgt tttgttgcga aaacaatcta atcataacta ccgcgaatac tcaatcatct
     4981 acaaaatgga ttaaatgtgc ttatttaata attaatttat atatttaatg cattaattct
     5041 taacattaat tgttcaataa tattcaccaa atcaatatca aaaaaaatcg caaaacatat
     5101 aattcaatac aaatcatcag gataggtttt gcaacgcgtg cattttgtcc cctttttcct
     5161 cgttgattag atgcaaaaat ttatgctgaa atatgtcaac cgatgaaaag cgtcggtagt
     5221 taagcagaaa ttaatatcgc ttactttaac caccgtagca caattagcta attttacgga
     5281 tgcagaactc acgctggcgg gacgttttta ttgcgtcagg gttgacatcc gtttttgtat
     5341 ccagtaactc taaaagcata tcgcattcat ctggagctga tttaatgact cacatcgttc
     5401 gctttatcgg tctactacta ctaaacgcat cttctttgcg cggtagacga gtgagcggca
     5461 tccagcatta agccagcacg cagtcaaaca aaaaacccgc gccattgcgc gggttttttt
     5521 atgcccgaag cgaggcgctc taaaagagac aaggacccaa accatgagcc agcaagtcat
     5581 tattttcgat accacattgc gcgacggtga acaggcgtta caggcaagct tgagtgtgaa
     5641 agaaaaactg caaattgcgc tggcccttga gcgtatgggt gttgacgtga tggaagtcgg
     5701 tttccccgtc tcttcgccgg gcgattttga atcggtgcaa accatcgccc gccaggttaa
     5761 aaacagccgc gtatgtgcgt tagctcgctg cgtggaaaaa gatatcgacg tggcggccga
     5821 atccctgaaa gtcgccgaag ccttccgtat tcataccttt attgctacct ccccaatgca
     5881 catcgccacc aagctgcgca gcaccctaga cgaggtgatc gaacgcgcta tctatatggt
     5941 gaaacgcgcc cgtaattaca ccgatgatgt tgaattttct tgcgaagatg ccgggcgtac
     6001 acccattgcc gatctggcgc gagtggtcga agcggcgatt aatgccggtg ccaccaccat
     6061 caacattccg gacaccgtgg gctacaccat gccgtttgag ttcgccggaa tcatcagcgg
     6121 cctgtatgaa cgcgtaccta acatcgacaa agccattatt tccgtacata cccacgacga
     6181 tttaggcctg gcggtcggca actcactggc ggcggtacat gccggtgcac gccaggtgga
     6241 aggtgcaatg aacgggatcg gcgagcgtgc cggtaactgt tcgctggaag aagtcatcat
     6301 ggcgattaaa gttcgtaagg atattctcaa cgtccacacc gccattaatc accaagagat
     6361 atggcgcacc agccagttag ttagccagat ttgtaatatg ccgatcccgg caaacaaagc
     6421 cattgttggc agcggcgcat tcgctcactc ctccggtatc caccaggatg gcgtgctgaa
     6481 aaaccgcgaa aactacgaaa tcatgacacc agaatctatt ggtctgaacc aaatccagct
     6541 gaatctgacc tctcgttcgg ggcgtgcggc ggtgaaacat cgcatggatg agatggggta
     6601 taaagaaagt gaatataatt tagacaattt gtacgatgct ttcctgaagc tggcggacaa
     6661 aaaaggtcag gtgtttgatt acgatctgga ggcgctggcc ttcatcggta agcagcaaga
     6721 agagccggag catttccgtc tggattactt cagcgtgcag tctggctcta acgatatcgc
     6781 caccgccgcc gtcaaactgg cctgtggcga agaagtcaaa gcagaagccg ccaacggtaa
     6841 cggtccggtc gatgccgtct atcaggcaat taaccgcatc actgactata acgtcgaact
     6901 ggtgaaatac agcctgaccg ccaaaggtca cggtaaagat gcgctgggtc aggtggatat
     6961 tgtcaccaac tacaacggtc gccgcttcca cggcgtcggc ctggccaccg atattgtcga
     7021 gtcctccgcc aaagccatgg tgcacgtact gaacaatatc tggcgtgccg cagaagtcga
     7081 aaaagagttg caacgcaaaa ctcaacacaa cgaaaacaac aaggaaaccg tgtgatgtcg
     7141 aagaattacc atattgccgt attgccggga gacggtattg gtccggaagt gatgacccag
     7201 gcgctgaaag tgctgaatgc cgtgcgcaac cgctttgcga tgcgcatcac caccagccat
     7261 tacgatgtag gcggcgcagc cattgataac cacgggcaac cactgccgcc tgcgacggtt
     7321 gaaggttgtg agcaagccga tgccgtgctg tttggctcgg taggcggccc gaagtgggaa
     7381 catttaccac cagaccagca accagaacgc ggcgcgctgc tgccgctgcg taagcacttc
     7441 aaattattca gcaacctgcg cccggcaaaa ctgtatcagg ggctggaagc attctgtccg
     7501 ctgcgtgcag acattgccgc taacggcttc gacatccagt gcgtgcgcga actgaccggc
     7561 ggcatctatt tcggtcagcc aaaaggccgc gaaggtagcg gacaatatga aaaagcgttt
     7621 gataccgagg tgtatcaccg ttttgagatc gagcgtattg cccgcatcgc gtttgaatct
     7681 gctcgtaaac gtcgccacaa agtcacctca atcgacaaag ctaacgtgct gcaatcctct
     7741 attttatggc gtgaaatcgt caacgagatc gccacggaat acccggatgt cgaactggcg
     7801 catatgtaca tcgacaacgc caccatgcag ctgattaaag atccatcaca gtttgacgtt
     7861 ctgctgtgct ccaacctgtt tggcgacatt ctgtctgacg agtgcgcaat gatcactggc
     7921 tcgatgggca tgttgccttc cgccagcctg aacgagcaag gttttggact gtatgaaccg
     7981 gcgggcggct cggcaccaga tatcgcaggc aaaaacatcg ccaacccgat tgcacaaatc
     8041 ctgtcgctgg cactgctgct gcgttacagc ctggatgccg atgatgcggc ttccgccatt
     8101 gaacgcgcca ttaaccgtgc attagaagaa ggcattcgca ccggggattt agcccgtggc
     8161 gctgccgccg ttagtaccga tgaaatgggc gatatcattg cccgctatgt ggcagaaggg
     8221 gtgtaatcat ggctaaaacg ttatacgaaa aattattcga cgctcacgta gtgtacgaag
     8281 ccgaaaacga aaccccgctg ttatatatcg accgccacct ggtgcatgaa gtgacctcac
     8341 cgcaggcgtt cgatggtctg cgcgcccacg gtcgcccggt acgtcagccg ggcaaaacct
     8401 tcgccactat ggatcacaac gtctctaccc agaccaaaga cattaatgcc tgtggtgaaa
     8461 tggcgcgcat ccagatgcag gagctgatta aaaactgcaa agaatttggc gtcgagctgt
     8521 atgacctgaa tcacccgtat caggggatcg tccacgtaat ggggccggaa cagggcgtca
     8581 ccttgccggg gatgaccatt gtctgcggcg actcgcatac cgccacccac ggcgcgtttg
     8641 gcgcactggc ctttggtatc ggcacttccg aagttgaaca cgtactggca acacaaaccc
     8701 tgaaacaggg ccgtgcgaag accatgaaaa ttgaagtcca gggcaaagcc gcgccgggca
     8761 ttaccgcaaa agatatcgtg ctggcaatta tcggtaaaac tggcagcgca ggcggcaccg
     8821 ggcatgtggt ggagttttgc ggcgaagcaa ttcgtgattt aagcatggaa ggtcgtatga
     8881 ccctgtgcaa tatggcgatc gaaatgggcg caaaagccgc tctggttgct cctgacgaaa
     8941 ccacctttaa ctatgtcaaa ggccgtctgc atgcgccgaa aggcaaagat ttcgacgacg
     9001 ccgttgccta ctggaaaacc ctgcaaaccg acgaaggcgc aactttcgat accgttgtca
     9061 ctctgcaagc agaagaaatt tcaccgcagg tcacctgggg caccaatccc ggccaggtga
     9121 tttctgtgaa cgacaatatt cccgatccgg cttcgtttgc cgatccggtt gaacgtgcgt
     9181 cggcagaaaa agcgctggcc tatatggggc tgaaaccggg tattccgctg accgaagtgg
     9241 ctatcgacaa agtgtttatc ggttcctgta ccaactctcg cattgaagat ttacgcgcgg
     9301 cagcggagat cgccaaaggg cgaaaagtcg cgccaggcgt gcaggcactg gtggttcccg
     9361 gctctggccc ggtaaaagcc caggcggaag cggaaggtct ggataaaata tttattgaag
     9421 ccggttttga atggcgcttg cctggctgct caatgtgtct ggcgatgaac aacgaccgtc
     9481 tgaatccggg cgaacgttgt gcatccacca gcaaccgtaa ctttgaaggc cgccaggggc
     9541 gcggcgggcg cacgcatctg gtcagcccgg caatggctgc cgctgctgct gtgaccggac
     9601 atttcgccga cattcgcaac attaaataag gagcacacca tggcagagaa atttatcaaa
     9661 cacacaggcc tggtggttcc gctggatgcc gccaatgtcg ataccgatgc aatcatcccg
     9721 aaacagtttt tgcagaaagt gacccgtacg ggttttggcg cgcatctgtt taacgactgg
     9781 cgttttctcg atgaaaaagg ccaacagcca aacccggact tcgtgctgaa cttcccgcag
     9841 tatcagggcg cttccatttt gctggcacga gaaaacttcg gctgtggctc ctcgcgtgag
     9901 cacgcgccct gggcattgac cgactacggt tttaaagtgg tgattgcgcc gagttttgct
     9961 gacatcttct acggcaatag ctttaacaac cagctgctgc cggtgaaatt aagcgatgca
    10021 gaagtggacg aactgtttgc gctggtgaaa gccaatccgg ggatccattt cgacgtggat
    10081 ctggaagcgc aagaggtgaa agcgggagag aaaacctatc gctttaccat cgatgccttc
    10141 cgccgccact gcatgatgaa tggtctggac agcatcggcc tgaccctgca gcacgacgac
    10201 gctattgcgt cttatgaagc aaaacaacct gcgtttatga attagacccc ttgcccggtc
    10261 aaatgaccgg gctttccgct atcgtccacg tcatcaaacg tctttaacct ttgcggttaa
    10321 aaataatgcg acaacagaaa taatcgcaat tacccagtag acagcaaagt gcccccaact
    10381 ttgtgcaatt gctccctgaa taacaccagc cagaattacc ccggtagaga tagtgttggt
    10441 aaataaggtg gtagctgccc ccgctcttcc aggcattaaa tcctgaaacc atagcatccc
    10501 aatacccgca acaatgccga taaatacagc gttaaaaagt tgcagcgtca tcaacgccat
    10561 acggctatga aagaaaatca atccagtgta aaacagcact cctgccacca ctgcaatgac
    10621 catcattcgc cgcttaccat aacgtttgac atagtagcca gccagaatca ttgctggtat
    10681 ttccagtcca gctgccgtcc ccatcaggaa acccgccagt ttgtctggca atcctaaatc
    10741 gctactgatc cacaatggca tatcaataat gtacatagtg ttgcaggtcc acattaacgt
    10801 cgaggcgaca aataacatcc gtacgttact atcctgccag tcaccttgca ttgataaagc
    10861 attttccgac ggcagttcta cccgcgccac agacagaagc ataaatgcaa tcaataccag
    10921 actgagtgtg aatatcccgg cggcaatcga aaacatcact gtaaagccgt aattcaacgc
    10981 cagcataaag gccagcggtg gaccgattac ccatgccaga gaaagctgcg cacgcatcac
    11041 cgagctaaac atcaccactt ctcgcgccga gttatccgcg tattcccgcg ccagagcaaa
    11101 taactgtggc attgccgtat tggccagaga tgccagaagc acaccacagg tgataagcgt
    11161 cagataatga cgattaaatg caaacagtag cgcattgcca atagccatca aacagcaaaa
    11221 tataatcagt tttcgccgat cgccctgact gtcagaacgt tttgccaacc agaggcttac
    11281 gccgatccca gcaatagcat tcaccgtata aaagagtccg atccagaaag gttgcgcgcc
    11341 aacctcacga ctcagaaata agctcaatgt aggagcctgt agcgccccgg ccacccccat
    11401 cataaaagcg accagcataa atgccgcgta aacaccgttc atacggcgag ccatcgtcat
    11461 tatccagatc atacgttccc tttttagcgc ggcgagaata aaaaaaacca gcaggtataa
    11521 tctgctggcg ggtgatttta caccaatact cagtcacaca tgatgcaggc aagtcaactt
    11581 tcagaattgc ggtcatccca ctgcatcagt ccttcaagca tggttaatcg ttgtgggacg
    11641 cttaaccaac gcaaccagca caacttcgct gtcgcggtaa aatagtgctg ataaaactga
    11701 cgcatggggc acctccttgc ttcatcgtta ggaattattg acttaatata gggaaaataa
    11761 aattgctgtc ttttgcgcag gagttcccct tttatgccat ctgctcgtct gcaacaacag
    11821 ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg acacaacgct caacgaactg
    11881 gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc tgctcaacac catgcaggat
    11941 cgcggctggc tgacgtggga agcggaagtc gggcgcggta aacgctcgcg tctgacattc
    12001 ctctataccg ggctggcgct tcagcaacag cgggcggaag acctgctgga gcaggatcgt
    12061 atcgaccaac tagtgcagtt ggttggcgac aaagcgactg tgcggcaaat gctggtttct
    12121 catctgggcc gcagcttccg ccaggggcgg cacatcctgc gcgtgctcta ctatcgtccg
    12181 ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg aaacccatat tgcccggcaa
    12241 atcttcagtt cgctaacgcg cataaatgag gaaaatgggg aactggaagc agacatcgcc
    12301 caccactggc agcaaatatc accgcttcac tggcgtttct ttttgcgtcc aggagtccat
    12361 tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg cctctttaaa acgaatcaat
    12421 acgctgccgc tctattcgca tattacggac attgtgtcgc cgacgccctg gacgctggat
    12481 atccatctca cgcagccgga ccgctggttg ccgttactgc tggggcaagt tccggcgatg
    12541 atcctgccgt gcgaatggga aaccctcagt aactttgcca gccatcctat tggcaccggt
    12601 ccgtatgcgg tgattcgcaa cagcaccaat caactgaaaa ttcaggcatt cgatgacttc
    12661 ttcggttacc gggcattaat cgacgaagtt aacgtctggg ttctgccgga aattgccgac
    12721 gagccagccg gtggtctgat gctaaaaggt ccacagggcg agaaaaaaga gattgaaagc
    12781 cgcctggagg aaggttgcta ctatttactg ttcgacagcc gcacccatcg cggggcgaat
    12841 cagcaagtca gggactgggt aagctatgtg ctttctccaa ctaatctggt ctatttcgct
    12901 gaggaacagt accagcaact gtggttcccg gcttatggac tgctcccccg ttggcaccat
    12961 gcccgcacca taaagagcga aaaaccggct ggcctggaaa gcctcaccct gaccttttat
    13021 caggatcaca gtgagcatcg ggtgattgcc gggatcatgc agcagattct ggcaagtcac
    13081 caggtcacgc tggaaatcaa agagatcagc tacgatcagt ggcatgaagg agagatcgag
    13141 agcgatatct ggcttaatag cgccaacttt acgctgccgc tggatttttc gctgttcgcg
    13201 cacttgtgcg aggtgccgct gctccaacac tgtattccga tcgactggca agccgacgcc
    13261 gcccgctggc gcaatggcga aatgaacctg gcgaactggt gccagcaact ggtcgccagt
    13321 aaagcaatgg tgccgcttat ccaccactgg ctgatcattc agggacaacg cagtatgcgc
    13381 ggcctgcgta tgaataccct cggctggttc gattttaaat cagcgtggtt tgcgccaccg
    13441 gatccatgat tgctggtaga taacaaaatc actacactaa cgccgttctc aacggggtgc
    13501 cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg atccggataa cgccggcgaa
    13561 gggatttgag gctccttctc aagtcctttg ccactctttt ttgaggtgca aagtgttaaa
    13621 aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt ttcgctaaac ccgttctgac
    13681 tgtttatacc tacgattcct tcgccgccga ctgggggcct ggtccaaaga tcaaaaaagc
    13741 ttttgaagcc aactgtaact gcgaactgaa actggtggcg ctggaagatg gcgtttcgct
    13801 tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc gatgtggtgc tggggctgga
    13861 taacaatctg ttagacgccg ccagtaaaac cggactattt gccaaaagcg gtgtggcagc
    13921 ggatgccgtt aacgttcccg gcggctggaa taatgacact ttcgtcccgt ttgattacgg
    13981 ctatttcgcc ttcgtctatg acaagaacaa actgaaaaac ccgccgcaaa gcctgaaaga
    14041 attggttgag agcgatcaga actggcgggt gatttatcag gatccgcgta ccagcacgcc
    14101 ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc gatgacgccc cacaagcctg
    14161 gcaaaaactg gcgaagaaaa ccgtcacggt caccaaaggc tggagcgaag cctacggcct
    14221 gtttttaaaa ggtgaaagcg atctggtact gagttacacc acctctccgg cttatcacat
    14281 tctcgaagag aaaaaagata actacgccgc cgcgaacttt agcgaaggtc actatctgca
    14341 ggtggaagtc gccgcccgca ccgctgccag caagcagccg gagctggcgc aaaaattcct
    14401 ccagtttatg gtttctccgg cttttcagaa tgcgatccca accggtaact ggatgtatcc
    14461 ggtggcaaat gtcacgctgc ctgccgggtt tgaacaattg accaaaccgg ccaccacgct
    14521 ggagttcaca ccagctgaag tggcggcaca acgtcaggca tggattagcg aatggcaacg
    14581 cgccgtcagc cgttaattcc cggctggtta attccaggtg taagcgccgc cacgctggtg
    14641 gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga acgcgccgca gggtaactgg
    14701 gtggcagtct ggcaggacag ctacctgtgg catgtggtgc gcttctcctt ctggcaggcg
    14761 tttctctcgg cgctactctc tgtcgtaccc gcgatattcc tcgcccgcgc cctctatcgc
    14821 aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg caatgacctt gatcctcccg
    14881 gtgctagttg ctgttttcgg cattcttagc gtctatggtc gtcagggctg gctggcatcg
    14941 ctctgccaat cgctcggtct ggagtggacc ttttcgccct acggcctgca aggcattttg
    15001 ctggcgcacg tattttttaa tctgccgatg gcgagccgct tattactcca ggcactggaa
    15061 aacattcccg gcgaacaacg tcagcttgcc gcccagcttg ggatgcgcgg ctggcatttt
    15121 ttccgcttcg tcgaatggcc gtggctacgg cgacaaatcc cgccggttgc cgcgcttatc
    15181 tttatgctct gtttcgccag cttcgccacc gtgctatcgc tgggcggcgg tccgcaggca
    15241 accactatcg agctggctat ttatcaggcg ctgagttacg actacgatcc tgcccgcgcg
    15301 gcgatgctgg cgctgctcca gatggtgtgt tgccttgggc tggtgctgtt gagtcagcga
    15361 ctaagtaagg ccattgcgcc cggcaccacg ctgctgcaag gctggcgcga tccggacgat
    15421 cgtctgcata gccgcatttg cgacacggtg ttaatagtgc tggcgctgct gctgttgctg
    15481 ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc agttgccgga agtgctggca
    15541 caaccggtgc tgtggcaggc gctgtggacc tcgttgcgta ttgcgctggc ggcaggtgta
    15601 ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc gcgaactgcg ggcgcggcag
    15661 aaaatgctgg cgggtcaggc gctggagatg agcggcatgt tgatcctcgc catgccgggg
    15721 attgtgctgg ctaccggctt ctttttactg ctcaacaaca ctatcggcct gccacaatct
    15781 gctgacggca ttgtgatttt caccaatgcg ttaatggcga tcccttatgc gctgaaagta
    15841 ctggaaaacc cgatgcgcga tatcaccgcc cgctacagta tgttgtgtca gtcgctgggc
    15901 attgaaggct ggtcgcgctt aaaagtggtc gagctgcgcg ccctgaaacg tccactggcg
    15961 caggcgctgg cttttgcctg cgtgctgtcg attggtgatt ttggcgtagt ggcgttgttc
    16021 ggtaacgatg atttccgtac cctgccgttt tatctctacc agcaaattgg ctcctatcgc
    16081 agccaggacg gcgcggtcac cgcgttaatt ctgctactac tctgttttct gctgtttacc
    16141 gtgattgaaa aactaccggg gcgaaatgtt aaaactgact gatatcacct ggctttatca
    16201 ccatttgccg atgcgtttta gcttaacggt agaacgcggc gagcaggtgg caatcctcgg
    16261 gccaagcggc gcgggtaaaa gtaccctgct gaatttgatt gccggttttc tgacgccagc
    16321 cagcggttcg ctgactatcg atggtgttga tcacaccacg acgccgccgt cacgccgtcc
    16381 ggtgtcgatg ctgtttcagg agaacaacct gttcagccat ctgacggtcg cgcagaacat
    16441 cgggctgggg ctaaatccgg ggttgaaact gaacgcggca cagcaggaga aaatgcacgc
    16501 gattgcccgt cagatgggga ttgataattt aatggcgcgg ttaccgggcg agctttccgg
    16561 cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggtg cgcgaacagc cgattttatt
    16621 gctcgatgaa ccgttctctg cgctcgatcc ggcgttacgt caggagatgc tgacgctggt
    16681 gagcacgagc tgccagcagc aaaaaatgac gctattgatg gtgtcgcaca gtgtggaaga
    16741 tgcggcgcgg atcgccacac gctcggtggt ggtcgccgac gggcgcatcg cctggcaggg
    16801 taagactgaa gaattgttga gcggtaaggc gagtgcttcg gcaatattgg ggattacggg
    16861 ttagtatgca ctgtcagatg cggcgtgaac accttatccg acctacggac agtgcgtttt
    16921 gtaggcctga taagacgcgg tcagcgtcgc atgaggcaac ccgcacaaga caatccccta
    16981 aaccccaacc actttacgca aaatatcgat atacaccggc atcaacgggt ggcgaattaa
    17041 caccaccagc gccaccacgc cgatggcaga aatcaacggc gtcagccaca acaaacggcc
    17101 gcgcgacaaa taatgactta aacgatcggt tgctttaccg ctgcgccata accgccagca
    17161 cagccagcca ccaacccaca aaaataccgc cgtcgccagc agcaaccatt taaactcgcc
    17221 gctctgcatt ccggctggaa tatcgatcgc cgcgcccgcc agaatcccag gcaggaagta
    17281 aaacggtggc cacagcaggc agccgataat attcggcgta atgaattttg ccaccggcag
    17341 atccagcatt cccgccacca ttggcaccag cggacgcgtc gggccaacaa agcgaccgac
    17401 cagaatggtg aacatgctgt gttgatgcaa cgcatgttca gttttatcga gtaatgcttt
    17461 gtttttcttc agaaatgacc agcgatgcaa cggcttttta aaacgccagc ccagccagaa
    17521 ggagatccag tcgcccagca gacaaccgac aatccctgcc agccaagcgt gccagaaact
    17581 taactcgccg ctgccaatca gcgctcccag ccccgccatc agcaccgtac cgggtagaat
    17641 caaaccgacc agcgccagcg actccagaaa ggccaccaac accaccgcca tcaatgaata
    17701 cacggtagat tgggtaataa agtgttccag caatgcttgc ataatgtgcc tgtcaaatgg
    17761 acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt gtctgattcg
    17821 ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga
    17881 actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt
    17941 caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct
    18001 tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc
    18061 ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat
    18121 caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acacgattat
    18181 ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa
    18241 gcagattcat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt
    18301 gcccaaacag gtcgctgaaa tacggctggt gcgcttcatc cgggcgaaag aaacccgtat
    18361 tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa
    18421 cccactggtg ataccattcg cgagcctccg gatgacaacc gtagtgatga atctctcctg
    18481 gcgggaacag caaaatatca cccggtcggc agacaaattc tcgtccctga tttttcacca
    18541 ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga
    18601 taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat
    18661 taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc
    18721 cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct
    18781 tttactggct cttctcgcta acccaaccgg taaccccgct tattaaaagc attctgtaac
    18841 aaagcaggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa
    18901 aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca
    18961 taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc
    19021 cgtttttttg gatggagtga aacgatggcg attgcaattg gcctcgattt tggcagtgat
    19081 tctgtgcgag ctttggcggt ggactgcgcc agcggtgaag agatcgccac cagcgtagag
    19141 tggtatcccc gttggcaaaa agggcaattt tgtgatgccc cgaataacca gttccgtcat
    19201 catccgcgtg actacattga gtcaatggaa gcggcactga aaaccgtgct tgcagagctt
    19261 agcgtcgaac agcgcgcagc tgtggtcggg attggcgttg acagtaccgg ctcgacgccc
    19321 gcaccgattg atgccgacgg taacgtgctg gcgctgcgcc cggagtttgc cgaaaacccg
    19381 aacgcgatgt tcgtattgtg gaaagaccac actgcggttg aagaagcgga agagattacc
    19441 cgtttgtgcc acgcgccggg caacgttgac tactcccgct atattggcgg tatttattcc
    19501 agcgaatggt tctgggcaaa aatcctgcat gtgactcgcc aggacagcgc cgtggcgcaa
    19561 tctgccgcat cgtggattga gctgtgcgac tgggtgccag ctctgctttc cggtaccacc
    19621 cgcccgcagg atattcgtcg cggacgttgc agcgccgggc ataaatctct gtggcacgaa
    19681 agctggggcg gcttgccgcc agccagtttc tttgatgagc tggacccgat cctcaatcgc
    19741 catttgcctt ccccgctgtt cactgacacc tggactgccg atattccggt gggcacctta
    19801 tgcccggaat gggcgcagcg tctcggcctg cctgaaagcg tggtgatttc cggcggcgcg
    19861 tttgactgcc atatgggcgc agttggcgca ggcgcacagc ctaacgcact ggtaaaagtt
    19921 atcggtactt ccacctgcga cattctgatt gccgacaaac agagcgttgg cgagcgggca
    19981 gttaaaggta tttgcggtca ggttgatggc agcgtggtgc ctggatttat cggtctggaa
    20041 gcaggccaat cggcgtttgg tgatatctac gcctggtttg gtcgcgtact cggctggccg
    20101 ctggaacagc ttgccgccca gcatccggaa ctgaaagcgc aaatcaacgc cagccagaaa
    20161 caactgcttc cggcgctgac cgaagcatgg gccaaaaatc cgtctctgga tcacctgccg
    20221 gtggtgctcg actggtttaa cggtcgtcgc acgccaaacg ctaaccaacg cctgaaaggg
    20281 gtgattaccg atcttaacct cgctaccgac actccgctgc tgttcggcgg tttgattgct
    20341 gccaccgcct ttggcgcacg cgcaatcatg gagtgcttta ccgatcaggg gatcgccgtc
    20401 aataacgtga tggcgctggg cggcatcgcg cggaaaaacc aggtcattat gcaggcctgc
    20461 tgcgacgtgc tgaatcgccc gctgcaaatt gttgcctctg accagtgctg tgcgctcggt
    20521 gcggcgattt ttgctgccgt cgccgcgaaa gtgcacgcag acatcccatc agcccagcaa
    20581 aaaatggcca gtgcggtaga gaaaaccctg caaccgcgca gcgaacaggc acaacgcttt
    20641 gaacagcttt atcgccgcta tcagcaatgg gcgatgagcg ccgaacaaca ctatcttcca
    20701 acttccgccc cggcacaggc tgcccaggcc gttgcgactc tataaggaca cgataatggc
    20761 gatttttgat aattatgaag tgtggtttgt cattggcagc cagcatctgt atggcccgga
    20821 aaccctgcgt caggtcaccc aacatgccga gcacgttgtt aatgcgctga atacggaagc
    20881 gaaactgccc tacaaactgg tgttgaaacc gctgggcacc acgccggatg aaatcaccgc
    20941 tatttgccgc gacgcgaatt acgacgatcg ttgcgctggt ctggtggtgt ggctgcacac
    21001 cttctccccg gccaaaatgt ggatcaacgg cctgaccatg ctcaacaaac cgttgctgca
    21061 attccacacc cagttcaacg cggcgctgcc gtgggacagt atcgatatgg actttatgaa
    21121 cctgaaccag actgcacatg gcggtcgcga gttcggcttc attggcgcgc gtatgcgtca
    21181 gcaacatgcc gtcgttaccg gtcactggca ggataaacaa gcccatgagc gtatcggctc
    21241 ctggatgcgt caggcggtct ctaaacagga tacccgtcat ctgaaagtct gccgatttgg
    21301 cgataacatg cgtgaagtgg cggtcaccga tggcgataaa gttgccgcac agatcaagtt
    21361 cggtttctcc gtcaatacct gggcggttgg ctatctggtg caggtggtga actccatcag
    21421 cgacggcgat gttaacgcgc tggtcgatga gtacgaaagc tgctacacca tgacgcctgc
    21481 cacacaaatc cacggcgaaa aacgacagaa cgtgctggaa gcggcgcgta ttgagctggg
    21541 gatgaagcgt ttcctggaac aaggtggctt ccacgcgttc accaccacct ttgaagattt
    21601 gcacggtctg aaacagcttc caggtctgcc cgtacagcgt ctgatgcagc agggttacgg
    21661 ctttgcgggc gaaggcgact ggaaaaccgc cgccctgctt cgcatcatga aggtgatgtc
    21721 aaccggtctg cagggcggca cctcctttat ggaggactac acctatcact tcgagaaagg
    21781 taatgacctg gtgctcggct cccatatgct ggaagtctgc ccgtcgattg ccgtagaaga
    21841 gaaaccgatc ctcgacgttc agcatctcgg tattggtggt aaggacgatc ctgcccgact
    21901 gatcttcaat acccaaaccg gtccagcgat tgtcgccagc ctgattgatc tcggcgatcg
    21961 ttaccgtctg ctggttaact gtatcgacac ggtgaaaaca ccgcactccc tgccgaaact
    22021 gccggtggcg aatgcactgt ggaaagcgca accggatctg ccaactgctt ccgaagcgtg
    22081 gatcctcgct ggtggcgcgc accataccgt cttcagccat gcgctgaacc tcaacgatat
    22141 gcgccagttc gccgagatgc acgacattga aatcacggtg attgataacg acacccgcct
    22201 gccagcgttt aaagacgcac tgcgctggaa cgaagtgtat tacggatttc gtcgctaagt
    22261 agccacatca ggtatgtaac gcctgatgcg acgctgacgc gtcttatctg gcctacacgc
    22321 tgcgattttg taggccggat aagcaaagcg catccggcat tcaacgcctg atgcgacgct
    22381 ggcgcgtctt atcaggccta cgcgctgcga ttttgtagac cggataagcg aagcgcatcc
    22441 ggcacgaagg agtcaacatg ttaaaagatc tcaaacgcca ggtattagaa gccaacctgg
    22501 cgctgccaaa acacaacctg gtcacgctca catggggcaa cgtcagcgcc gttgatcgcg
    22561 agcgcggcgt ctttgtgatc aaaccttccg gcgtcgatta cagcgtcatg accgctgacg
    22621 atatggtcgt ggttagcatc gaaaccggtg aagtggttga aggtacgaaa aagccctcct
    22681 ccgacacgcc aactcaccgg ctgctctatc aggcattccc ctccattggc ggcattgtgc
    22741 atacgcactc gcgccacgcc accatctggg cgcaggcggg tcagtcgatt ccagcaaccg
    22801 gcaccaccca cgccgactat ttctacggca ccattccctg cacccgcaaa atgaccgacg
    22861 cagaaatcaa cggcgaatat gagtgggaaa ccggtaacgt catcgtagaa acctttgaaa
    22921 aacagggtat cgatgcagcg caaatgcccg gcgttctggt ccattcccac ggcccgtttg
    22981 catggggcaa aaatgccgaa gatgcggtgc ataacgccat cgtgctggaa gaggtcgctt
    23041 atatggggat attctgccgt cagttagcgc cgcagttacc ggatatgcag caaacgctgc
    23101 tggataaaca ctatctgcgt aagcatggcg cgaaggcata ttacgggcag taatgactgt
    23161 ataaaaccac agccaatcaa acgaaaccag gctatactca agcctggttt tttgatggat
    23221 tttcagcgtg gcgcaggcag gttttatctt aacccgacac tggcgggaca ccccgcaagg
    23281 gacagaagtc tccttctggc tggcgacgga caacgggccg ttgcaggtta cgcttgcacc
    23341 gcaagagtcc gtggcgttta ttcacgccga tcaggttccc cgcgctcagc atattttgca
    23401 gggtgaacaa ggctttcgtc tgacaccgct ggcgttaaag gattttcacc gccagccggt
    23461 gtatggcctt tactgtcgcg cccatcgcca attgatgaat tacgaaaagc gtctgcgtga
    23521 aggtggcgtt accgtctacg aggccgatgt gcgcccgcca gaacgctatc tgatggagcg
    23581 gtttatcacc tcaccggtgt gggtcgaggg tgatatgcac aatggcacta tcgttaatgc
    23641 ccgtctgaaa ccgcatcccg actatcgtcc gccgctcaag tgggtttcta tagatattga
    23701 aaccacccgc cacggtgagc tgtactgcat cggcctggaa ggctgcgggc agcgcatcgt
    23761 ttatatgctg gggccggaga atggcgacgc ctcctcgctt gatttcgaac tggaatacgt
    23821 cgccagccgc ccgctgctgc tggaaaaact caacgcctgg tttgcaaacc acgatcccga
    23881 tgtgatcatc ggctggaacg tggtgcagtt cgatctgcga atgctgcaaa aacatgccga
    23941 gcgttaccgt cttccgctgc gtcttgggcg cgataatagc gagctggagt ggcgcgagca
    24001 cggctttaaa aacggcgtct tttttgccca ggctaaaggt cggctaatta tcgacggtat
    24061 cgaggcgctg aaatccgcgt tctggaattt ctcttcattc tcgctggaaa ctgtcgctca
    24121 ggagctatta ggcgaaggaa aatctatcga taacccgtgg gatcgaatgg acgaaattga
    24181 ccgccgtttc gccgaagata aacctgcgct ggcaacttat aacctgaaag attgcgagct
    24241 ggtgacgcag atcttccaca aaactgaaat catgccattt ttactcgaac gagcaacggt
    24301 gaacggcctg ccggtggatc ggcacggcgg ttcggtggca gcgtttggtc atctctattt
    24361 tccgcgaatg catcgcgctg gttatgtcgc gcctaatctc ggcgaagtgc cgccgcacgc
    24421 cagccctggc ggctacgtga tggattcacg gccagggctt tatgattcag tgctggtgct
    24481 ggactataaa agcctgtacc cgtcgatcat ccgcaccttt ctgattgatc ccgtcgggct
    24541 ggttgaaggc atggcgcaac ctgatccaga gcacagtacc gaaggttttc tcgatgcctg
    24601 gttctcacgg gagaaacatt gcctgccgga gattgtgact aacatctggc acgggcgcga
    24661 taaagccaaa cgtcagggta ataagccgct ttcgcaggcg ctgaagatca ttatgaatgc
    24721 cttttatggc gtgctcggca ccaccgcctg ccgcttcttc gatccgcgcc tggcatcgtc
    24781 gatcaccatg cgtggtcatc agatcatgcg gcagaccaaa gcattgattg aagcgcaggg
    24841 ctacgacatt atctacggag ataccgactc gacttttgtc tggctaaaag gtgcacattc
    24901 ggaagaagag gcggcgaaaa tcggtcgtgc actggtgcag cacgttaacg cctggtgggc
    24961 ggaaacgctg caaaaacaac ggctgaccag cgcattagaa ctggagtatg aaactcattt
    25021 ctgccgtttt cttatgccaa ccattcgcgg agctgatacc ggcagtaaaa agcgctacgc
    25081 cgggctgatt caggagggcg acaagcagcg gatggtgttt aaagggctgg aaaccgtgcg
    25141 caccgactgg acgccgctgg cccagcagtt tcagcaggag ctgtacctgc gtatcttccg
    25201 caacgagcca tatcaggagt acgtccgcca aaccatcgac aaactgatgg cgggtgaact
    25261 ggatgcgcgg ctggtttacc gtaaacgcct tcgccgtccg ctgagcgaat atcagcgtaa
    25321 tgttccgcct catgtacgcg ccgctcgcct tgccgatgaa gaaaatcaaa agcgcggtcg
    25381 ccccttgcaa tatcagaatc gcggcaccat taagtacgta tggaccacca acggcccgga
    25441 gccgctggac taccaacgtt caccactgga ttacgaacac tatctgaccc gccagctaca
    25501 acccgtggcg gagggaatac tcccttttat tgaggataat tttgctacac ttatgaccgg
    25561 gcaacttggg ctattttgag caaaaaaaag agttcgccag ataccatttt gatgcgtgac
    25621 gaatgctttg ccatccagta ccatagcgcc ctttccattc ctggacctga ataacaccac
    25681 tacctcataa gcacggtagc gggtggttat tgcctgcaat taaagatata gagccgaaca
    25741 catatgcctt ttacacttgg tcaacgctgg atcagcgata cagaaagcga attgggactt
    25801 ggaaccgttg tcgcggtgga tgcgcgaact gtcactttac ttttcccatc tactggtgaa
    25861 aaccgtctgt acgcacgcag tgattccccc gtgacccgcg tgatgttcaa ccctggtgat
    25921 accattacca gccatgacgg ctggcagatg caagtcgaag aagtaaaaga agaaaatggc
    25981 ttgctgacct atatcggtac tcgcctggat actgaagagt ccggcgtagc cctgcgtgaa
    26041 gttttccttg atagcaaact ggtgttcagc aaaccgcagg accgtctgtt tgccgggcag
    26101 attgaccgta tggaccgctt tgcgctgcgt tatcgcgcgc gtaagtattc cagcgaacag
    26161 ttccgtatgc cgtacagcgg cctgcgcggt cagcgtacca gcctgatccc gcatcagctc
    26221 aacatcgctc atgatgttgg tcgccgccac gcgccgcgcg tcctgctggc tgacgaagtg
    26281 ggtttaggga aaaccattga agccgggatg atcctgcatc agcaactgct ctctggcgct
    26341 gctgaacgtg tgctgattat cgtcccggaa accttacagc atcagtggct ggtagaaatg
    26401 ctgcgccgtt tcaacctgcg ctttgctctg tttgatgatg agcgttatgc cgaagctcag
    26461 cacgatgctt acaacccgtt tgacaccgaa cagctggtga tttgctcgct ggattttgcc
    26521 cgtcgtagca aacagcgctt ggaacatctc tgtgaagccg aatgggacct gctggtggtc
    26581 gatgaagcgc atcacctggt gtggagcgaa gatgcgccaa gccgtgaata tcaggccatt
    26641 gaacaactgg cagagcacgt gccgggcgtt ctgctgctga ccgcgacccc ggaacagctg
    26701 gggatggaaa gccacttcgc ccgtctgcgt ctgctggacc cgaaccgttt ccacgatttt
    26761 gcgcagttcg ttgaagagca gaaaaattat cgtccggttg cggacgccgt tgccatgctg
    26821 ctggcaggta acaaactgag caatgacgaa ctgaacatgc tcggcgagat gatcggcgag
    26881 caggatatcg agccgctgtt gcaggcagca aacagcgaca gcgaagatgc ccagagcgcc
    26941 cgtcaggagc tggtttcgat gctgatggat cgccacggca ccagccgcgt gctgttccgt
    27001 aacacgcgta acggtgtgaa aggattcccg aaacgcgagc tgcacaccat taagctgccg
    27061 ctaccgacgc agtatcagac ggctattaaa gtctccggca ttatgggcgc acgtaaaagt
    27121 gcggaagatc gtgctcgcga tatgctctac ccggagcgta tttatcagga atttgaaggt
    27181 gataacgcca cctggtggaa cttcgatccg cgcgttgagt ggctgatggg ctacctgacc
    27241 agccatcgct ctcagaaagt gctggtgatc tgcgccaaag ctgccactgc gctgcaactg
    27301 gagcaggtac tgcgcgaacg tgaaggtatt cgcgctgcgg tgttccacga aggtatgtcg
    27361 attatcgaac gtgaccgcgc tgccgcctgg tttgccgaag aagacaccgg cgcacaggta
    27421 ctgctgtgct cagaaatcgg ttctgaagga cgtaactttc agttcgccag tcacatggtg
    27481 atgtttgacc tgccattcaa cccggatcta ctggagcagc gtattggtcg tctggatcgt
    27541 atcggccagg cgcacgatat tcagatccat gtgccttatc tggagaaaac cgctcagtcg
    27601 gtgctggtgc gctggtatca cgaaggtctg gatgcatttg agcacacctg cccgaccgga
    27661 cgcactattt acgatagcgt atacaacgat ctgattaact atctggcttc accggatcaa
    27721 accgaaggct ttgacgatct gatcaaaagc tgccgcgagc aacatgaagc gctgaaagca
    27781 cagctggaac agggtcgtga ccgcctgctg gaaatccact ccaacggtgg cgaaaaagcc
    27841 caggcactgg cagaaagcat tgaagagcag gatgacgata ccaacctgat cgccttcgcc
    27901 atgaacctgt tcgatattat cggtatcaat caggacgatc gcggcgacaa catgatcgtg
    27961 ctgacgccgt ccgatcatat gctggtgccg gacttccctg gcctgtcgga agatggcatc
    28021 accatcacct ttgatcgtga agtggcgctg gcgcgtgaag atgcacagtt tattacctgg
    28081 gagcatccgc tgatccgcaa cggtctggat ctgatccttt ctggcgatac cggtagcagc
    28141 acgatttcac tgttaaaaaa caaagcgttg ccggtaggta cgctgttggt ggaactgatt
    28201 tatgtggttg aagcccaggc tccgaagcag ttgcagctca accgcttcct gccaccgacg
    28261 ccggtacgta tgctgctgga taaaaacggc aacaacctgg cggcgcaggt agagtttgaa
    28321 acctttaacc gccagcttaa cgcggttaac cgtcacaccg gcagcaaact ggttaacgcc
    28381 gtgcagcagg atgttcacgc tatccttcaa ctgggtgaag cgcagatcga gaaatctgcc
    28441 cgtgcattga ttgatgcagc gcgtaacgaa gccgacgaaa aactgtctgc cgagctgtct
    28501 cgtctggaag ctctgcgtgc agtgaacccg aacattcgtg acgacgaact gaccgccatt
    28561 gagagcaacc gtcagcaggt aatggaaagc ctggatcagg caggttggcg tctggatgcc
    28621 ctgcgtttga tcgttgtaac gcatcagtaa cggagccgaa aatggggatg gaaaactaca
    28681 atccaccgca ggaaccctgg ttggttatcc tgtatcagga tgaccatatt atggtggtca
    28741 acaagccgag cggtttgttg tcagtgccgg gtcgtctgga agagcacaaa gacagcgtga
    28801 tgacgcgcat tcagcgtgat tatccgcagg cagaatcggt gcatcgtctg gatatggcta
    28861 ccagcggcgt gattgtagtg gcgctgacca aagccgcgga gcgggagtta aaacgccagt
    28921 tccgcgagcg cgagccgaaa aagcagtatg tggcccgcgt ctgggggcat ccatcccccg
    28981 cagaaggtct ggtggatctg ccgctgattt gcgactggcc aaaccgcccg aaacagaaag
    29041 tctgttacga aacgggtaaa cctgcgcaga cggaatatga agtggtggag tatgcggcgg
    29101 ataacacggc aagagtggtg ttaaaaccga ttaccgggcg ttcgcatcaa ttgcgtgtgc
    29161 atatgctggc gctggggcat ccgattctcg gcgatcgttt ttatgcatca ccagaagcga
    29221 gagcgatggc accgcgtttg ttgctacatg cagagatgtt gacgattacc catccggcgt
    29281 atggcaatag tatgacgttt aaagcgccag cggattttta atcgtcacgc tggcgcagtt
    29341 cggtaggcgg ataaggcgtt cacgccgcat ccggcaattg tgcgccgatt gcctgatgcg
    29401 acgcgggccg cgtcttatca ggcctacagt ttaaaactta tttaaaccct ttctgctgct
    29461 ttatcagctc atatgcctgc tgaatttcct gcgctttctg cttcgccatc tccatcatct
    29521 caggcggcaa acctttcgcc accagcttat cgggatggtg ttcactcatc agcttacggt
    29581 aggcacgttt gatggtggtc gcatcatccg tcggcttcac gcccagcaca ttacaggcat
    29641 cttccagcgt cgggccacgc tgcgcttgct gccagttacc accgccagat tgctgctgat
    29701 aaccgccgcc aaactgtgca ccgccctgca tcatgcgcaa aaactggtca aactgggcgc
    29761 gggagatccc taattcttca gcaatgacat acagcaccgc ccgttcattc gggtgcagtg
    29821 aaccatcagc aaacgccgcc tgaatctgga tctccagaaa catacgaatt aagtcaaaac
    29881 gaccaaagca gacactgcga aactggcgca tcttttcgcg cagcgggtaa ttatctgatt
    29941 ttcccacccg gaacgcgttt tgcgccgcag tacgggaagc gccatgaagg ttcattcggt
    30001 ccatcaactg gctggcgata tgaatatcag cctccgtgac gcgacctttg gatttggtta
    30061 aatgccccat cacttcaaaa gtggtggcaa aaaacagcgc ctgacgctca cgctggttgg
    30121 cgaaccacgc cattttacgg ctacgggctt tatcaaacat atggccaatt aacaggccta
    30181 acactacgcc ccaaaagccg ccgcccatca gtaaggccac ggccacgcca atgatttttc
    30241 cccaatactg catatattcc ccaaatcgac acacggatat cagggctatc tcccacaata
    30301 taaaggtgct tttaccgttt tccggctgcg gtcaattgtg ggacatcgcc tataatttgc
    30361 attatcatac ctgtcattca atgccgtgcc taacaccact gacgctattc ggacaggatt
    30421 aacactagcg tagagatgac gagtacgtta gtctctgacc gtttgtcacg cgcaacgtta
    30481 ccgatgatgg aacaataaaa tcaacgtatg aaaaaacgta tccccactct cctggccacc
    30541 atgattgcca ccgcccttta tagtcaacag ggactggcag ccgacctcgc ctcacagtgc
    30601 atgttgggcg tgccaagcta tgaccgtcct ctggtacagg gcgataccaa tgacttaccc
    30661 gtgactatca atgctgacca cgcgaaaggg gactacccgg atgacgccgt gtttactggc
    30721 agcgtggata tcatgcaggg taacagccgt ctgcaggccg acgaagtgca gctccatcaa
    30781 aaagaggcac caggacaacc ggagccggta cgtaccgttg atgcgctcgg taatgtccat
    30841 tacgacgata accaggtgat cctcaaaggg ccgaaaggct gggcgaatct gaacaccaaa
    30901 gataccaacg tctgggaagg tgattaccag atggtgggtc gccagggtcg cggtaaagcg
    30961 gacctgatga aacaacgtgg cgaaaaccgc tataccattc tggataacgg tagctttacc
    31021 tcctgtctgc cgggttctga cacctggagc gtggtaggta gcgaaattat tcatgaccgc
    31081 gaagaacaag ttgctgagat ctggaacgcc cgctttaagg tgggtccggt accgatcttt
    31141 tatagcccct atttgcagtt gccggtgggt gacaaacgtc gctctggttt cttgatcccg
    31201 aacgccaagt acaccaccac caactacttt gagttctacc tgccatatta ctggaacatc
    31261 gcgccaaata tggatgccac catcacgccg cattatatgc atcgtcgtgg caacatcatg
    31321 tgggagaacg aattccgcta cctctcccag gcgggcgctg gcttgatgga actggactat
    31381 ctgccttcag ataaagtcta tgaagatgaa cacccgaacg atgacagttc acgtcgttgg
    31441 ttattctact ggaaccactc cggggtcatg gatcaggtgt ggcgtttcaa cgtcgactac
    31501 accaaggtca gcgatcctag ctacttcaat gatttcgata acaagtacgg ttccagtact
    31561 gacggctacg caacgcaaaa attcagcgtt ggctatgcgg tgcaaaactt caatgccacc
    31621 gtttcaacca agcagttcca ggttttcagc gaacagaaca ccagtagcta ctcggcagag
    31681 ccgcagttag acgttaatta ctaccagaat gatgttggtc cgtttgatac gcgtatttac
    31741 ggccaggcag tgcactttgt taacaccaga gacgacatgc ctgaagcaac ccgtgttcac
    31801 ctggaaccga ccatcaattt gccgctctct aataactggg gcagcatcaa taccgaagcg
    31861 aagtttctgg caacccatta tcagcaaacc aatcttgact ggtataactc cagaaacacg
    31921 accaagctgg acgaatccgt taaccgcgta atgccgcaat tcaaagttga cggcaaaatg
    31981 gtctttgaac gcgatatgga aatgctggct ccgggttata cccaaacgct ggaaccgcgc
    32041 gcgcagtatt tgtacgtgcc gtatcgcgat cagagcgaca tctataacta cgactcgtct
    32101 ctgctgcaat ctgactactc tggcctgttc cgggaccgga cttacggcgg tcttgaccgt
    32161 attgcctccg ctaaccaagt gacgaccggt gtcacatctc gcatatatga tgatgctgcc
    32221 gttgaacgtt ttaatatttc cgttggtcaa atctactatt tcacggagtc tcgcactggc
    32281 gatgacaaca taacatggga gaatgacgac aaaacgggct cactggtgtg ggcaggcgac
    32341 acctactggc gtatctccga gcgttgggga ttgcgtggcg ggattcagta cgatacacgt
    32401 ctggataacg tagcgaccag taactccagc attgaatacc gtcgggatga agaccgtctg
    32461 gtacagctga attaccatta cgccagcccg gaatatattc aggctacgct gcctaagtac
    32521 tattccactg ctgagcaata taagaatggt atttcgcagg taggtgctgt cgccagcagg
    32581 ccaattgccg atcgttggtc cattgttggg gcctactact acgacaccaa tgcgaacaag
    32641 caagccgact ctatgttagg tgtgcaatac agctcctgct gctatgcaat tcgcgtcggt
    32701 tacgagcgga agctgaacgg ttgggataac gataaacaac atgcggtata tgacaacgca
    32761 atcggcttta acatcgaact tcgcggcctg agctccaact acggtctggg tacgcaagag
    32821 atgctgcgtt cgaacattct gccgtatcaa aacactttgt gatttgttga tttaccacgt
    32881 aatccgcagt gcggttaatt gaaatggaaa aagtatgaag aactggaaaa cgctgcttct
    32941 cggtatcgcc atgatcgcga ataccagttt cgctgccccc caggtagtcg ataaagtcgc
    33001 agccgtcgtc aataacggcg tcgtgctgga aagcgacgtt gatggattaa tgcagtcggt
    33061 aaaactgaac gctgcgcagg caaggcagca gcttcctgat gacgcgacgc tgcgccacca
    33121 aatcatggaa cgtttgatca tggatcaaat catcctgcag atggggcaga aaatgggagt
    33181 gaaaatctcc gatgagcagc tggatcaggc gattgctaac atcgcgaaac agaacaacat
    33241 gacgctggat cagatgcgca gccgtctggc ttacgatggt ctgaactaca acacttatcg
    33301 taaccagatc cgcaaagaga tgattatctc tgaagtgcgt aacaacgagg tgcgtcgtcg
    33361 catcaccatc ctgccacagg aagtcgaatc cctggcgcag caggtgggta accaaaacga
    33421 cgccagcact gagctgaacc tgagccacat cctgatcccg ctgccggaaa acccgacctc
    33481 tgatcaggtg aacgaagcgg aaagccaggc gcgcgccatt gtcgatcagg cgcgtaacgg
    33541 cgctgatttc ggtaagctgg cgattgctca ttctgccgac cagcaggcgc tgaacggcgg
    33601 ccagatgggg tggggccgta ttcaggagtt gccggggatc ttcgcccagg cattaagcac
    33661 cgcgaagaaa ggcgacattg ttggcccgat tcgttccggc gttggcttcc atattctgaa
    33721 agttaacgac ctgcgcggcg aaagcaaaaa tatctcggtg accgaagttc atgctcgcca
    33781 tattctgctg aaaccgtcgc cgatcatgac tgacgaacag gcccgcgtga aactggagca
    33841 aattgctgct gatatcaaga gtggtaaaac gacttttgct gccgcagcga aagagttctc
    33901 gcaggatcca ggttctgcta accagggcgg tgatctcggc tgggctacac cagatatttt
    33961 cgatccagcc ttccgtgacg ccctgacccg cctgaacaaa ggtcaaatga gtgcaccggt
    34021 tcactcttca ttcggctggc atttaatcga actgctggat acccgtaatg tcgataaaac
    34081 cgacgctgcg cagaaagatc gtgcataccg catgctgatg aaccgtaagt tctcggaaga
    34141 agcagcaagc tggatgcagg aacaacgtgc cagcgcctac gttaaaatcc tgagcaacta
    34201 atggttaaaa cccaacgtgt tgtgatcact cccggcgagc ccgccgggat tggcccggac
    34261 ttagttgtcc agcttgcaca gcgtgagtgg ccggtcgaac tggttgtttg tgccgatgcc
    34321 actctcctta ccgaccgggc agcgatgctc ggtttgccgc tcaccctccg cccttattcc
    34381 cccaactccc ctgcacaacc gcaaactacg ggcacattaa cgctacttcc tgtcgcgcta
    34441 cgtgaatctg tcactgcggg gcagttagcg attgaaaatg ggcattacgt ggtggagacg
    34501 ctggcgcgag cgtgtgatgg ctgtctgaac ggtgaatttg ctgcgctgat cacaggcccc
    34561 gtgcataaag gcgtcattaa cgacgcaagc attccgttta ccggtcatac cgagtttttc
    34621 gaagagcgtt cgcaggcgaa aaaagtggtg atgatgctgg cgaccgaaga acttcgcgtg
    34681 gcgctggcaa cgacgcattt accgctgcgc gatatcgcag atgctatcac ccctgcactt
    34741 ttgcacgaag tgattgctat tttgcatcac gatttgcgta caaaatttgg tattgccgaa
    34801 ccgcgcattc tggtctgcgg gctgaatccg cacgcgggcg aaggcggtca tatgggtacg
    34861 gaagagatag acaccattat tccggtgctc gacgagctgc gggcgcaggg gatgaaactc
    34921 aacgggccgc tgcctgccga taccctgttt cagccgaaat atctcgataa cgccgacgcc
    34981 gtgctggcga tgtaccacga tcagggtctt cccgtgctaa aataccaggg cttcgggcgc
    35041 ggtgtgaaca ttacgctggg cctgcccttt attcgcacat cagtggacca cggcaccgcg
    35101 cttgaactgg cgggacgtgg cgaagccgat gtcggcagtt ttattacggc gcttaatctc
    35161 gccatcaaaa tgattgttaa cacccaatga ataatcgagt ccaccagggc cacttagccc
    35221 gtaaacgctt cgggcaaaac tttctcaacg atcagttcgt gatcgacagc attgtttctg
    35281 ccattaaccc gcaaaagggc caggcaatgg tcgaaatcgg ccccggtctg gcggcattga
    35341 ccgagccggt cggcgaacgt ctggaccagc tgacggtcat cgaacttgac cgcgatctgg
    35401 cggcacgtct gcaaacgcat ccattcttag gcccgaaact gacgatttat cagcaggatg
    35461 cgatgacctt taactttggc gaactggccg agaaaatggg tcagccgctg cgtgttttcg
    35521 gcaacctgcc ttataacatc tccacgccgt tgatgttcca tctgtttagc tatactgatg
    35581 ccattgccga catgcacttt atgttgcaaa aagaggtggt gaatcgtctg gttgcaggac
    35641 cgaacagcaa agcgtatggt cgattaagcg tcatggcgca atactattgc aacgtgatcc
    35701 cggtactgga agttccgccg tcagccttta caccaccacc caaagtggat tccgccgtcg
    35761 tgcgcctggt ccctcatgca acgatgcctc acccggttaa agatattcgc gtgttgagcc
    35821 gcatcaccac cgaagccttt aaccagcgtc gtaaaaccat tcgtaacagc ctcggcaacc
    35881 tgtttagcgt cgaggtgtta accggaatgg ggatcgaccc ggcgatgcga gcggaaaata
    35941 tctctgtcgc gcaatattgc cagatggcga actatctggc ggagaacgcg cctttgcagg
    36001 agagttaacg atgatcaatt cgccccgagt gtgtattcag gttcaaagcg tctacattga
    36061 ggcacaatct tcacctgata atgaacgtta cgtttttgct tataccgtaa ccatacgcaa
    36121 tctggggcga gcgccagtgc agttgttggg gcgttactgg ctgatcacca atggcaatgg
    36181 ccgtgaaacc gaagtccagg gcgaaggagt ggttggcgtc cagccactta tcgcgcctgg
    36241 cgaagagtac cagtacacca gcggtgcaat cattgaaacc ccgctgggca ccatgcaggg
    36301 tcactacgaa atgatcgatg aaaatggcgt ccctttcagc atcgacattc ccgtattccg
    36361 actcgccgtt cccacactca ttcattaaaa gaatatggcg acatacctta ttggcgacgt
    36421 tcatggttgt tacgatgaac tgatcgcatt gctgcataaa gtagaattta cccccgggaa
    36481 agataccctc tggctgacgg gcgatctggt cgcgcgcggc ccgggttcgc tggatgttct
    36541 gcgctatgtg aaatccttag gcgacagcgt acgtctggtg ctgggcaatc acgatctgca
    36601 tctgctggcg gtatttgccg ggatcagccg caataaaccg aaagatcgcc tgacaccgct
    36661 gctggaagcg ccggatgccg acgagctgct taactggctg cgtcgccagc ctctgctgca
    36721 aatcgacgaa gagaaaaagc tggtgatggc ccacgcaggg atcacgccgc agtgggatct
    36781 gcagaccgcc aaagagtgcg cgcgcgatgt agaagcggtg ctgtcgagtg actcctatcc
    36841 cttctttctt gacgctatgt acggcgatat gccaaataac tggtcaccgg aattgcgggg
    36901 gctgggaaga ctgcgtttta tcaccaacgc ctttacccgt atgcgttttt gcttcccgaa
    36961 cggtcaactg gatatgtaca gcaaagaatc gccggaagaa gcccctgccc cactgaaacc
    37021 gtggtttgcg attcctggcc ctgtcgctga agaatacagc atcgcctttg gtcactgggc
    37081 atcgctggaa ggcaaaggta cgccggaagg tatttacgcg ctggataccg gctgctgctg
    37141 gggtgggtca ttaacctgcc tgcgctggga agataaacag tattttgtcc agccgtcgaa
    37201 ccggcataag gatttgggcg aagcggcggc gtcttaaaca cagcctgata caggaaggcc
    37261 ggataagacg cgaccggcgt cgcatccggc actagccgta aattctatac aaacttaccg
    37321 ccgctccaga atctcaaagc aatagctgtg agagttctgc gcatcggcgt cgtggaattc
    37381 gctgaatacc gattcccagt catccggctc gtaatccggg aaatgggtgt cgccttccac
    37441 ttctgcgtcg atatgcgtca gatacagttt ttgcgctttt ggcaggaact gctcataaac
    37501 gcgaccgccg ccaatcacca tgatttctgg tacgtcacca cacgccgcaa tggcttcatc
    37561 caccgatttc acccacgtta cgcgatcgtc cgtacccggt tgactgctga ggataatatt
    37621 tttgcgtcct ggcaacggac gaccgattga ttcccaagta tggcggccca taatcacggg
    37681 tttatttaag gtgttgcgtt taaaccaggc gagatcggca ggcaggttcc acggcatggc
    37741 gttttccatg ccgataacgc gatctaccgc taacgccgca atcagactga tcattgagat
    37801 ttcccgataa aaaaattgtc gccactatac gtaaagcgta aaccgtcgtc gactggtgcg
    37861 aggatgatgt tgaggaaaat tttatattct gctggcgagt ccacgctctc cccctggact
    37921 cgccgcatta caatgaaaca aaaacaaaca gttagctgta aagtgtgatt tacgtcactc
    37981 tttattaaga tgagggtttc gtttccggtt catccgccat gttgccggta tgtttacctt
    38041 cttctgttcc ctgccagcca tgtcgttgaa ttaatgacag atgttcgcgg tcctcggtga
    38101 tgatctcact taacatcgcg ctggtgcgtt tatagaccgc cgcgcgggct ttggtgtcat
    38161 tctcaaccat tgccatctct tccaccatct gaatattaaa gcggcggaac acatcggcac
    38221 gttctcgcgc ttcatacggc cccagaccta aactttccag cgccagacgc ccggttttca
    38281 gcgcaccttc aaaggtttca cgctccggct tttcaacgcc tgcctgacgc aaacgaatgt
    38341 agtgatcgac atcgcgggcg cgggcaataa tctgcaaatg cgggaaatgt tctttcacca
    38401 tctccgtcag ttgcaggttg gtttgcggat cgtcaatagc gttaatcagc acttccgctt
    38461 tcgccgctcc ggcagattcc agtaaatcca tccgcgtggc atcgccataa aacactttca
    38521 taccaaattt acgcagcgtt tcgatatggt ccggatcgtg atcgagcacc accattttca
    38581 ccccgctgga gagcagtaaa cgtccggtaa tctgtccaaa acgaccgaat ccggcgataa
    38641 tcacgcgcgg ctgttcttca tcgatctcat cggcttcacg cgcctcctcc gtagaagatt
    38701 gctcaaggcg attgaggatc accagcagaa tcggcgttgc tgccatcgac agcgccaccg
    38761 ccagggtcag cgatttcgcc cactccggct ccagcacatt cgccatctgc gccgcaccaa
    38821 ataccacaaa ggcaaactca ctgccctgcc ctaacaacac cgcaaaccag cgacgctgtt
    38881 tatttggcac ctgcaacggt cgggcaatca gccacagcat ggcgattttg atgatgagga
    38941 aaccgagcag caaaatgaca atgcgcaatg ggttttcaat cagcgtgcca aagtctatcg
    39001 acatgccaac accgatgaaa aacagcccca acagcaaacc tttaaacggt tcgatatcgc
    39061 tttccaacgc atgacggtat tccgagcttg ccagcagtac gcccgccaga aacgcgccca
    39121 tcgccatcga caagccgacc tcttccagca gcaaaccaaa gccaaacaca aggaataacg
    39181 ccacggcact aaacacttcc cgcaagccag agcgggcaac aaaacgcagc gccggacgcg
    39241 taacatagcg ccccagcaat accaccagta ccagcgcacc cgccactttt aacgccgaga
    39301 gagcaaatgc gcccatcgtc gtcgaggcac tgctcgccgc cagtagcggg atcatcgcca
    39361 ccagcgggat tgccgcgata tcctggaaca gcagcactgc aaaggcactg cggcccattt
    39421 gcgtcaccat cagattgcgt tcattcatcg cctgcatggc aatcgccgtt gatgagagcg
    39481 ccagcgtcat accgatcaat tccgcgacct gccagcgcag cccaagtaac atacagaaca
    39541 gccccagcag gccaccgcaa atcaccatct gcaatgcgcc accgccgaac actgccgcac
    39601 gcagcttcca cagcctttgt ggatcgagtt cgaggccgat aataaacagc atcagcacca
    39661 ccccaatctc ggcaaagtgc agaatagatt cggcatcggt caccagtcgc agcccccacg
    39721 ggccaataat gcagccggcg atcaggtagc caagtaccga tcccagacca agacgtaccg
    39781 caatgggtac aatcagcgcc gccgatccga ggtagatcag cgcctgaacc agcgtatgac
    39841 tatccatgat gggcctcctg ccattccagc agacgttgct tatagtggcg cgcctgccct
    39901 tcgagggttt cgtcgtcaca aataaaggtg cagtgcatgg caaacggtgg cagccagttc
    39961 agcccgcagt agattgccgt cgcctgtagc ggctgcgaca gcacatcaaa gcccggatgc
    40021 gcaccaattt caaaatggct ttccccgccg ccggtcgtca ccgcccacag caaatgtttg
    40081 ccatgcagcg ccgtgccgcc gtgaccgtaa gcccagccgt gcgacaaaac tttatcgatc
    40141 caaagtttaa gaagcggagg aatgctgtac cactgcatcg ggtgctgcca gacgatcaga
    40201 tcggcgcgag agagcgcctc ctgctcggcg gcaatatcga tattgaagtc aggatagagt
    40261 tgataaagag agcgaatttc gacgccttcc agcgtccttg cctgttcaag catccgttta
    40321 ttcgcatggg aatgatgcgg atacggatgc gcataaatga tgaggatcat agttattagc
    40381 ccgcgtgttt acgcctttgt tttcccacaa agtgtagtca tatattcacg cggcttatag
    40441 tcaataatat tgattcagaa aagagagaaa tctgaacaaa taatcccatt ctctgcccgc
    40501 ggatcgtcat attgctggtc tgcgccagtg acattcctcg agtctccggg gcaaacgcta
    40561 cggaaatcaa caagccaaac agcgagatac ccgcccccat tagcatcgtg ttactgatac
    40621 cgtaattatt gataaatatc ggcagtgccc aggtcgaaac aatggtgcca atacgactta
    40681 aggacataat cacgcccacg gcagaggcgc ggatatccgt cgggaagagt tcattaggat
    40741 agagccactg caaattaccc ggcccgccag agaaaaaggc atacaccgca aaggccatca
    40801 ctaccagcca gatccccata tccgggatta gccccaaaac cgccaacgcc agcgtcatca
    40861 tggcaaagct gccaatcaac aatggacgcc gtccggcagt gtttagccac agcatcggcg
    40921 gaatacagcc gagcataaag aacaggctaa tcaccacatt ccctagtgcc gcgtttttgc
    40981 caacccccaa tcccaacgaa ccaacgattt gcgggccaaa ggtgtaaatg gcgaacattg
    41041 ggatcacctg gcaggtccag atggcggcaa caaacagaac aaaaggaaaa tggcggcgat
    41101 taaacagatc gcgaaaacgg gtttgctgcg gctgctcttc atcgaaagcc accggttcgc
    41161 caaacagttt tatcatcatc tcttcgcact cttttactcg ccctttgcgt aataaccagc
    41221 ggggagattc tggcagttcg aatcgaccaa tcaaaatcaa caaacaaggg atcgccgcgc
    41281 tacccagcat ccagcgccaa ccaccttcca catcataaag ccagtagccg accagatcgg
    41341 cgcaggtcgc gccgacatac cacatcgcgg caataaagct gatggaaaac gcccgctgac
    41401 gggtactgga gaactcggtg atcatcgaag tggcgatggg ataatctgca ccgatgacaa
    41461 tgccgatgag tacccgcatc accaacagtt cgacggggga tgaaacacac atcgtcgcca
    41521 ccgatatcac gccgatggcg atgatatcaa tgaggaacat tttgcgccgt ccgactttat
    41581 cggagatata gccaaacagc gatgtgccaa cgaacagccc ggcgagcgtt cccgcgccca
    41641 gcaagccaat ccagtcagcg tccagtttca gcgccggagt cagttgctcc agcgccacgc
    41701 caatcattac cagtacataa ccatccagaa acggaccacc gcttccccac agcaaaatgc
    41761 ggcggtgaat agaggagaat ttgagatcgt caaagtttct ggacggttgc atgggttatt
    41821 cctgtttttt tgcaatgaga gtcagtacgg cgttacgtat caaaccaaca tcagccgtaa
    41881 cggaactcca caccaaaggt gccgcgcggg tattcccact gttccagcgc cgaccccagc
    41941 cccagaatgc gacaggtgcc gcactccaga catccggcgt aatcgaagcg cacactgccg
    42001 tcatcctgct tcttgtacag acctgcgggg cacgctttca ccagcagctc cagcacctgt
    42061 ttatcagcat cggccttcac aacaatgtgc ggatgctctt catcgacatt gaatttattg
    42121 acgcccagtt tgacgtccac attgacggga gaagtcataa aacggtcact cctttcatgc
    42181 catccttgat cagattgatg aagcccactt tcttgccgtg gcggaggatt ttcttgcgca
    42241 tcagttccgg cgcactgccg tcaatggtga acaggtcacg cgccacgccc accgccagtt
    42301 ccgggttgcc gctaaacatg cgtgggttat caaggaacgc cggtagtttc tggtacatgc
    42361 gcatatcgcg cagcgggcca ctctccagat gctgacgata ttccgcgagt ttttgcttac
    42421 tgaaatcgtc gcttttcatc gctgaaagca cggtttttgc tgcagcttcc ccggcggcaa
    42481 tcgccagatc cataccgcga atggtaaaac cgaggttcat acacattccg gcggcatcac
    42541 cggcaatcaa tacgccgtca ccgaccagct ccggcagcat gttgatgcct gcttccggca
    42601 ctacgtgagc ggaatattcc accagcttgc cgcccgcgat cagcggtgca acggccggat
    42661 gctgtttgaa atcttccagc atttgcggca ccgatttttt cgcgtcatgc agatgatgca
    42721 gaccacaaac cagccccagc gacagggtat tttcattggt ataaaggaag ccgccgccca
    42781 tcaggccatc ggtgggtgat cccgcaaaca gacaagccgc gccctgatta ccctgcaact
    42841 gaaaacggtc ttcgattacc gacttcggta actcgatcag ttccttcacg ccaaccgcca
    42901 catccgtcgg tttaacgcgt tttgccatcc ccaacttttc ggcaaggatg gagttcaccc
    42961 catcagcaag gagcaccgtt ttcgcttcaa tcacatcacc atcggcttct acaccgacga
    43021 ctttgccatc gcgctgtacg aggttatcga cgcggatccc ggtaattaac tgcgcgcccg
    43081 cttcttcggc ctgctccatc aaccaggcat caaatttact gcgcaaaacg gagtaagaac
    43141 gctgggatgg cgaggtttcg tcaccgttgc agtagtccat agtcatcgct gacttttccg
    43201 tcataaacgc gagtttttca tgggtgatca ggcgttctac gggggcggaa tcggcgaaac
    43261 ctggaataat gtgttccaga ctgtgggcat agagacgccc gccagtaacg ttcttggcac
    43321 ctgcggaatt gccacgctcg ataactaaca cttgcgcccc ttcgcgggcg agcaccagtg
    43381 cggcaaccga accggcaagc cctgcaccga cgatgatggc gtcaaagata tcttcggaca
    43441 taagagctcc ctgtcagcgg cagagccgta atgcagccct gccagagtgg atcaacgcgc
    43501 taaagctgcg gtcagtgccg gaaggatctt cacggcatcg ccaacaatgc cgtaatccgc
    43561 gtactggaag atcggcgcat ttttatcttt attgatggcg aaaatggttt gcgacgcgtt
    43621 agcgccaacc atgtgctgga tctacccgga gatccccacc gccaggtaca gttcaggttt
    43681 cagcatcagg ttggagatac cgacatagcg ttcgtgctcc atccattttt cgttttctgc
    43741 caccggacga gaacaggcca actccgcacc tatcgccttg caaagctgtt ctgccagcgc
    43801 aatgttctct ttgctgccaa tgccgcgacc gacgctgacc accagacggg ctttgtcgag
    43861 atcgacgctg ttgctctggc gcgcctgggt tgccgtgcgg gtaatcgcca cagccggagc
    43921 ctgccactcc acggtgtgcg tttcgccagt gcgtgacgcg tctggctgag ccgcatcgaa
    43981 cgtgccgctg ctgatggtca gtaccgcata cggcgtggca atgcgttctt cgccaatcgc
    44041 cagaccgccg taaaccatgt gtttcactgt tgctttaccg tcctgtacgc tgacggtgct
    44101 ggcatcgtta gacaccgccg ctttaaggcg ataacccagt tttgccgcca gtaatttgcc
    44161 gcgacgggtg tttggcaaca gcaccagacc gtcagcgccg tgctggcgaa tagtgtcagc
    44221 catgacaccg gcgtaatctt cgatcatccg atcgtccggt ttgccgttta atttccagac
    44281 atgattagcg ccgagctgga ttgcctgtgc gccgtcggca tcattgagga caaaggcatt
    44341 gatttgatta gctaaagcct gcgcaccgtt catcagttcc ggcagacgag agggggtatc
    44401 gctgaatacc cagacttgag aaaacgtgtt catagcatcc cctgtaatta aatgactttg
    44461 cgaagatttt cagcaaatgc ggcgatctgt tcttcgccgt cgccttcaat cacgatgcgc
    44521 tgacgctcgc gctgtttcgg cgcggcaacc tgttgttcag accaggcgtc cacggcgtta
    44581 aaaccaatat ccgccgccga ccatacctgg actggctttt tcgccgcgcc gagaatggct
    44641 ttcatcgaag gaatttgtgg ggagttgata tcagtggaaa cagcaacaac cgcaggcagc
    44701 ggaatgctta aggtttcggt ttcatcttcc agttcgcgct caacggtgag ggtatctgcc
    44761 gtcagggaga taattttgct gacgccgtta actgccggaa tattgaggat ttcgcccacc
    44821 agcagaccaa cctgctgggc ataaaggtcg gaagaaccat cgccacagag gatcagatca
    44881 aagcctgctt tctgggcggc tgcagccagt gcgctcgccg tttgttgcgg cagtgcctgc
    44941 tcgaactggt catcaatcac cacaatcagt tcatccgggc cgcgcgatag cacatcttta
    45001 cgccctttgg cgttggtcag ggctttaccg cccacactta aggctgtcac ctgcgcctct
    45061 gctgcctgtt gctttagctg gcaagccgct tcaatagcgt tgagatcgta ttggcttatt
    45121 ttggcatcgg ctttgctgaa gtctaatgaa ccatcagcat tattgaccgc aatatcctgt
    45181 tcatcaggca cgcacttata gcaagtaata atcttcattg catctccaga aatcatgaag
    45241 gaaacgtcgt tcatatcggt ggtgttaatt acagatatca cgccctttaa tttggaaata
    45301 aaactttaat caccaatatt gaaagtcaga tgctattttt ttacccacaa aagaatataa
    45361 caaactgatt aataaccaaa attaacgaaa aacgccctct gttcactcga aacaaaaatg
    45421 tgataccaat cacagaatac agcttattga atacccatta tgagttagcc attaacgcgt
    45481 ccacgatgtt aataataatt atattaaatg ttaacaaaaa taaaacaaac gggaacgcaa
    45541 aataaatatt cgttttcaca gtggaattaa ctcatgaaga atgaaaagag aaaaacggga
    45601 atagaaccga aggttttctt tccgccatta ataatcgtcg gcatactttg ttggcttaca
    45661 gtcagagatc tggatgcggc gaatgtcgtt attaatgctg tattcagtta cgtcaccaat
    45721 gtatggggat gggcatttga atggtatatg gtggtgatgc ttttcggttg gttctggctg
    45781 gtgtttggcc cgtatgccaa aaagcgttta ggtaacgaac cgccagaatt tagcaccgcc
    45841 agttggatct ttatgatgtt cgcctcctgt acgtctgctg ccgtactgtt ctggggatcg
    45901 attgagatct actactacat ctccaccccg ccgtttggct tagaaccgaa ctcgacagga
    45961 gcgaaagagt tggggctggc ttacagcttg ttccactggg gacctctgcc gtgggccact
    46021 tacagcttcc tttcagtcgc cttcgcttac ttcttctttg tccgcaaaat ggaagtgatt
    46081 cgccccagct cgacactggt gccgctggca ggtgaaaaac acgccaaagg gttgttcggt
    46141 accatcgtcg acaacttcta tctcgtcgcc ttgatcttcg cgatgggtac cagtctgggc
    46201 cttgccacgc cgctggtgac cgagtgtatg caatggttgt ttggcattcc gcataccctg
    46261 caactggacg ctatcatcat tacctgctgg attatcctca acgccatttg cgtcgcttgc
    46321 ggtctgcaaa aaggggtacg tatcgccagt gacgtgcgta gttacctgag cttcctgatg
    46381 ctgggttggg tgttcatcgt cagcggtgcc agcttcatca tgaactactt caccgattcg
    46441 gtggggatgt tgctgatgta tctgccgcgc atgttgttct ataccgatcc catcgctaaa
    46501 ggcggcttcc cgcagggctg gaccgtgttc tactgggcat ggtgggtgat ttacgccatc
    46561 cagatgagta tcttcctcgc ccgcatctcc cgtggtcgta ctgtgcgtga actgtgcttc
    46621 ggcatggtga tggggctgac agcatccacc tggatcctgt ggaccgtgct cggtagtaac
    46681 actctgctgt tgatggataa aaacatcatc aacattccaa atctgatcga acagtacggt
    46741 gtggcgcgcg ccatcatcga aacctgggcc gctctgccac tcagcaccgc caccatgtgg
    46801 ggcttcttca tcctctgctt tattgccacc gttacgctgg ttaacgcctg ctcttatacc
    46861 ctggcgatgt ctacttgccg cgaagtacgc gatggtgaag aaccaccgct gctggtgcgt
    46921 atcggttggt caattctggt tggcattatc ggtattgttc tgctggcgct cggcggcctg
    46981 aaaccgattc aaaccgccat tatcgccgga ggatgcccgc tgttcttcgt caacattatg
    47041 gtgacgctct cctttattaa agacgcgaaa cagaactgga aagattaatt aacccccaaa
    47101 atatcaagag gttgaaagat ggattttaat ttaaatgatg agcaggaact gtttgtcgcc
    47161 ggtatccgcg aactgatggc cagcgaaaac tgggaggcct attttgccga gtgcgaccgt
    47221 gacagcgtct acccggaacg ttttgtcaaa gcactggcgg atatgggtat cgacagtctg
    47281 ctgatccctg aagagcacgg tggtctggac gcggggtttg ttactctcgc cgccgtgtgg
    47341 atggagctgg gacgtctggg ggcaccaacc tatgtactgt accagctgcc gggcgggttc
    47401 aataccttcc tgcgcgaagg cacacaagag cagatcgaca agattatggc tttccgcggc
    47461 accggtaagc agatgtggaa ctcagcgatt accgaaccgg gcgcgggctc cgacgtgggt
    47521 agcctgaaaa cgacttatac ccgtagaaat ggtaagattt atcttaatgg tagtaagtgt
    47581 tttattacca gtagcgccta caccccgtac atcgtggtga tggcgcgcga cggggcttct
    47641 ccggacaaac ctgtctacac cgaatggttt gttgatatga gcaaaccggg catcaaagtg
    47701 accaaacttg agaagctcgg tctgcgtatg gatagctgct gtgaaatcac ctttgacgat
    47761 gtggaactgg acgagaaaga catgttcggt cgggaaggta acggctttaa ccgcgtcaaa
    47821 gaagagttcg accatgaacg tttcctggta gccctgacca actacggtac ggcgatgtgc
    47881 gcctttgaag atgctgcgcg ctacgccaac cagcgcgtgc agtttggcga ggctattggt
    47941 cgtttccagt tgattcagga aaaattcgcc cacatggcga tcaaattaaa ctccatgaaa
    48001 aacatgctgt atgaagcagc gtggaaagca gacaacggca ccatcacctc tggcgatgca
    48061 gcgatgtgca aatacttctg cgccaatgcg gcatttgaag tggtggatag cgcaatgcag
    48121 gtgctgggcg gtgtcgggat tgcgggcaac caccgcatca gccgcttctg gcgtgacctg
    48181 cgtgtagacc gcgtttccgg aggatctgac gaaatgcaga tcctgacgct gggtcgtgcg
    48241 gtgctgaagc aataccgcta agtccgtgcg ctgcctgatg cgacgctgac gcgtcttatc
    48301 aggcctacgg gagcacgaat gtaagccgga taaggcgttt acgccgcatc cggcagtcat
    48361 gcgctacata aatttttcag gagctaataa tggatcatct acccatgccg aaattcggac
    48421 cgctggccgg attgcgcgtt gtcttctccg gtatcgaaat cgccggaccg tttgccggac
    48481 aaatgttcgc agaatggggc gcggaagtta tttggatcga gaacgtcgcc tgggccgaca
    48541 ccattcgcgt tcaaccgaac tacccgcagc tctcccgccg caatttgcac gcgctgtcgt
    48601 taaatatttt caaagatgaa ggccgcgaag cgtttctgaa attaatggaa accaccgata
    48661 tcttcatcga agccagtaaa ggtccggcct ttgcccgtcg tggcattacc gatgaagtac
    48721 tgtggcagca caacccgaaa ctggttatcg ctcacctgtc cggttttggt cagtacggca
    48781 ccgaggagta caccaatctt ccggcctata acaccatcgc ccaggccttc agtggttacc
    48841 tgattcagaa cggtgatgtt gaccagccaa tgcctgcctt cccgtatacc gccgattact
    48901 tttctggcct gaccgccacc acggcggcgc tggcagcact gcataaagtg cgtgaaaccg
    48961 gtaaaggcga aagtatcgac atcgccatgt atgaagtgat gctgcgtatg ggccagtact
    49021 tcatgatgga ttacttcaac ggcggcgaaa tgtgcccgcg catgagcaaa ggtaaagatc
    49081 cctactacgc cggttgcggt ctgtataaat gtgccgacgg ctacatcgtg atggaactgg
    49141 tgggcattac ccaaattgaa gagtgcttta aagatattgg cctcgcacat ctactcggta
    49201 cgccggaaat cccggaaggc actcagctta tccaccgtat cgaatgccct tacggcccac
    49261 tggtagaaga aaaactcgat gcctggctgg cggcacatac catcgcagaa gttaaagaac
    49321 gctttgccga actgaatatc gcctgtgcca aagtactgac cgtaccggaa ctggaaagca
    49381 atccacagta tgttgcccgt gaatcaatca ctcagtggca aacgatggat ggtcgcacct
    49441 gcaaagggcc gaacatcatg ccgaaattca aaaataaccc cggacaaatc tggcgcggaa
    49501 tgccctcaca tggcatggac acggctgcca ttttgaaaaa tatcggctac agcgaaaacg
    49561 acattcagga gttggtcagc aaaggtctgg ccaaagttga ggactaaacg cgtggcgtca
    49621 gtcggcttgc ctgactagcg cccgcccaat aacaaacgaa caaaaatgga tagaggtgca
    49681 atggatatca ttggcggaca acatctacgt caaatgtggg acgatctggc ggacgtttac
    49741 ggtcataaaa cggcgctgat ttgtgaatcc agcggcggag tcgttaaccg gtatagttat
    49801 cttgagttaa atcaggagat taaccgcacg gcaaacctgt tttatacgct ggggattcgc
    49861 aaaggcaaca aggttgcact acatctcgac aactgcccgg aatttatctt ttgctggttc
    49921 gggctggcaa aaattggcgc gattatggtg ccgattaacg cccgcctgtt acgcgaggaa
    49981 agcgagtgga tcttgcaaaa tagccaggcg tgcctgctgg tgaccagtgc gcaattttat
    50041 cctatgtatc aacagattca gcaggaagat gccactcaat tgcggcacat ttgcctgaca
    50101 gatgtggcac ttcccgctga tgatggagtg agttcattta ctcaactgaa aaatcaacaa
    50161 cctgccacct tgtgctatgc accgccgcta tcgactgacg atacggcgga aattctcttc
    50221 acctccggca ccacctcccg accgaaaggt gtggtgatta cccattacaa cctgcgcttc
    50281 gctggatatt actccgcctg gcagtgtgca ctgcgtgacg atgacgtcta catgacggta
    50341 atgcctgcgt ttcatatcga ttgccagtgt actgcggcga tggcggcgtt ttctgccggg
    50401 gccacctttg tgctggtcga gaaatacagc gcccgcgcct tctggggaca ggcgcagaag
    50461 taccgcgcca ccattaccga atgtattccg atgatgatcc gtacgttgat ggtgcagccg
    50521 ccttcagcga acgatcggca acaccgcctg cgggaagtga tgttttatct caacttgtcg
    50581 gagcaggaaa aagacgcatt ttgtgaacgc ttcggcgttc gcttgctgac gtcttatggg
    50641 atgacggaaa ccattgtggg cattatcggc gatcgcccta gcgataaacg acgctggccg
    50701 tcgattggtc gggcggggtt ttgctacgaa gcggagatcc gcgacgatca caatcgcccg
    50761 ctcccggcag gtgagatcgg tgaaatctgt attaaaggcg taccagggaa aaccatcttc
    50821 aaagagtatt ttctcaaccc gaaagccact gcgaaagtgc tggaagccga cggctggcta
    50881 cataccggcg ataccggata ccgcgacgaa gaaggctttt tttatttcgt cgatcgccgc
    50941 tgcaatatga tcaaacgcgg tggcgagaat gtctcctgcg tggagctgga aaatatcatc
    51001 gccacccatc cgaaaattca ggacatcgtg gttgtgggta ttaaagattc gattcgcgat
    51061 gaagccatca aagcatttgt ggtgctgaat gaaggtgaaa cattgagcga agaggaattt
    51121 tttcgcttct gcgaacaaaa tatggcgaaa tttaaagtgc cctcttatct ggagatcaga
    51181 aaagatctgc cacgtaattg ctcggggaaa ataattagaa agaatctgaa ataaatgaaa
    51241 cgtcagggaa ctaccctgcc agctaataat cacgccctaa aacaatatgc attttttgca
    51301 gggatgttgt cgtccctaaa aaagcaaaaa tggagaaaag gaatgagtga atcattacat
    51361 ctgacccgca atggatcaat tctggaaatt acccttgatc gcccaaaagc gaatgctatt
    51421 gatgcaaaaa ccagctttga aatgggcgaa gtatttctaa atttccgtga cgatccgcaa
    51481 ttacgtgtcg ccattattac cggtgccgga gagaaattct tttccgcggg ctgggattta
    51541 aaagcggcag cagaaggcga agcaccggat gctgactttg gcccgggtgg ttttgcggga
    51601 ttaaccgaaa ttttcaatct cgacaaacca gttatcgcag ctgtgaacgg ctacgccttt
    51661 ggcggcggtt tcgaactggc gctggcggca gattttattg tttgtgccga taacgccagc
    51721 ttcgccctgc cggaagccaa actgggcatc gttcctgaca gcggcggtgt gctgcgtctg
    51781 ccgaagatcc tgccgcctac catcgtcaat gaaatggtga tgaccggcag acgaatgggc
    51841 gcagaagagg cgctgcgttg ggggatagtc aaccgcgtgg ttagccaggc ggaactgatg
    51901 gataacgccc gcgaactggc tcagcagctg gttaacagcg ccccgctggc gattgcggcg
    51961 ctgaaagaga tctaccgcac caccagcgaa atgccggtag aagaagcgta tcgctatatt
    52021 cgcagcggcg tgttgaaaca ctatccatcg gttctgcatt cggaagatgc cattgaaggg
    52081 ccgctggcgt ttgccgagaa gcgcgatccg gtgtggaaag gacgttaaca accgtgagct
    52141 attacgcctt tgaggggtta attccggtgg ttcacccgac ggcgtttgtc catcccagtg
    52201 ccgtcttgat tggcgatgtg attgtgggag ccggtgtcta catcggccca ctcgcctcac
    52261 tgcgtggtga ctacgggcgg ttgatcgtgc aagcgggagc caatattcag gatggctgca
    52321 ttatgcatgg ctactgcgac actgacacca tcgttgggga aaacggccat atcgggcatg
    52381 gagcgatcct gcatggttgt gtgattggtc gcgatgcatt ggtcgggatg aacagcgtaa
    52441 ttatggatgg cgcggtcatt ggcgaagaga gcattgttgc cgcaatgagc tttgtcaaag
    52501 caggctttag tggcgagaaa cgccagttgt tgatgggtac gccagcccgc gctgtacgca
    52561 gtgttagtga cgacgagtta cactggaaac ggttgaatac caaagagtat caggatcttg
    52621 tcgggcgctg tcatgcatcg ttacatgaaa cgcagccact gaggcaaatg gaggaaaatc
    52681 gcccccgttt gcaggggacg acggatgtga cgcctaaacg gtgatcttgc cggatgcgac
    52741 ctggttgctg gataagatgc tacaacatcg tatctggcaa gcaggacgcc cggtatcaac
    52801 gagataaaat taacgacgca tactctttga caacatcatc tgccacttct gctcgcggtt
    52861 aagttccgtg agcggaacgg caggaatacg cggtgtcttt cttacgccga ctaatttttc
    52921 ccgactgtta ttgcgcaggc gcgcgtaaat ttgctcatcg atatcgacca ctttaaccag
    52981 tcgctgacac tggcatcccc gcccttccag cttattaggg atcatcttaa cttcgcagct
    53041 tatttctgtg acttccgaca gaatataagt cagggtatta accgccttgc tgtgttcaat
    53101 atcgaaatga atagagatct ctcttgctat cacccaccga ttttcagcca tcatccattc
    53161 ggcgattaac aagtagagtg gtttttcaac atatccttca cacatacgaa ctccagtttc
    53221 acaaattaaa agtctagcta tgactcgttt tgaagcaatt aaacaaggcc atattaaaat
    53281 tgtggatata tcgatagtat gcaacttcac cgtcgataag tgtgagctaa atcctgctta
    53341 tgtcatcaaa aatatcgatt ccccaaaaga tttacttaat gggcaaaaaa aaacggtcct
    53401 catcagagaa ccgtattagt tattcgatca aattagcgga tgaaaaatat ccgccacgac
    53461 acgctattat ttgatctgtg cgtgcatttc ctgcaccgaa attacttttt cagtcgcatc
    53521 ggcattcagc gccatcgcgg tagcgaaacc accgttcagg gtggtgtcat aatgcacttt
    53581 atattgcagc gcactgcgac ggatcacgcg ggagtcttca atcgcacgac ggcctgaggt
    53641 ggtgttgatg atgtaggtat attcgccatt cttgatacgg tcctgaatgt gcggacggcc
    53701 ttcatgcacc ttgtttacca gacgcggatt gatacccgct tcgcccagca caatcgccgt
    53761 gccgtgggtc gcatccagct cgaagccctg tttcagcagt tttgccgcca ggtccaccac
    53821 gcgttcttta tcgccttcgc gcacggaaag cagcgcacga ccgtgtttct tcatggtgga
    53881 gttgctgccc agctgcgctt tggcgaacgc ttcagcgaag gtgcggccca cgcccatgac
    53941 ttccccggta gagcgcattt ctggccctaa cagcgggtca acgcctggga atttattgaa
    54001 cggcagcacc acttctttca ccgagtagta cggcggaata acttctttgg ttacgccctg
    54061 ctcagccagc gatttgccag ccatcacacg cgccgccact tttgccagcg gtacgccggt
    54121 ggctttggag acgaacggaa cagtacgcgc cgcacgcggg tttacttcaa tcaggtaaac
    54181 ttcgttgttt ttcaccgcaa actgcacgtt catcagaccg cgcacctgca attcgaaggc
    54241 cagtttctgc acctgctggc gcatcacatc ctgaatttcc tgacttaagg tgtacgctgg
    54301 cagcgaacat gcggagtcac cggagtgcac acccgcctgc tcgatgtgct ccatgatgcc
    54361 gccaatcagc accatttcgc cgtcgcagat ggcatccacg tcaacttcta ctgcgtcatc
    54421 aaggaaatga tccagcaaca ctggcgcatc gttagacacg ctaaccgccg tctggaagta
    54481 gcgacgcagg tcagcttcgt catagacgat ttccatcgcc cgaccgccga gaacgtaaga
    54541 cggacgcacc accagcgggt agccaatctc ttttgccttc tcaactgcca tttcaatagc
    54601 agtaacggtg gcgttcgccg gttgtttcag tttcagacgg tcaaccgcat gctggaagcg
    54661 ttcacggtct tccgcacggt cgatagcatc cgggctggtg ccgataaccg gtacgccagc
    54721 ggcttccagt gcacgcgcca gtttcagcgg ggtctgaccg ccgtactgaa cgataacgcc
    54781 tttcggcttc tcaatacgca cgatttccag cacatcttcc agagttaccg gctcgaagta
    54841 gaggcgatcg gaagtgtcgt agtcggtaga gacggtttcc gggttacagt taaccataat
    54901 ggtttcgtaa ccgtcttcac gcagcgccag cgaggcgtgt acgcagcagt agtcgaattc
    54961 gataccctga ccgatacggt ttggaccgcc gccaagcacc atgatttttt cacggtcggt
    55021 agacggattc gcttcgcact cttcttcata agtggagtac atgtaagcgg tgtcggtggc
    55081 aaactctgcc gcacaggtat ccacgcgctt gtagaccggg tgcaggtcat attggtcacg
    55141 cagcttacgg atttccgctt cgcgtacgcc cgccagtttt gccaagcgcg catcggcaaa
    55201 gcctttgcgt ttcagctggc gcaggaattc agcgttcagg ccagtgatgc ccacttccgc
    55261 cactttctct tccagacgca ccagttcttc aatctgtacc aggaaccagc ggtcaatgtt
    55321 agtcaggttg aagacgccgt ccacagacag gcccgcgcgg aaagcatcgg cgatgtacca
    55381 gatacgctct gcgcctgcgt ctttcagttc gcgacggatt ttggttaacg cttccgggtc
    55441 atccaggctc actttcgggt cgaagccagt tgcgcccact tccaggccgc gcagcgcttt
    55501 ttgcagggat tcctgctgcg tgcgaccaat cgccatcact tcgccaaccg atttcatctg
    55561 agtggtcaga cggtcgttag caccggcgaa tttttcgaag ttgaagcgag gaattttagt
    55621 aaccacatag tcgatggacg gctcgaagga ggccggagta cgtccgccag tgatgtcgtt
    55681 catcagttcg tctagggtgt aacccaccgc cagtttcgcc gccactttag caatcgggaa
    55741 accggtcgct ttcgacgcca gcgccgaaga acgggacacg cgtgggttca tttcgataac
    55801 aatcaggcga ccgtttttcg ggttcaccgc aaactggaca ttggaaccac cggtttcaac
    55861 gccgatttca cgcagcaccg ccatcgaggc gttacgcatg atttgatatt ctttgtcggt
    55921 cagcgtttgg gctggcgcga cagtgatgga gtcaccggtg tggatgccca tcgcatcaaa
    55981 gttttcgata gagcagacga tgatgcagtt gtcgttttta tcacgcacca cttccatctc
    56041 gtactctttc cagccgatca gcgactcatc aatcagcaac tctttggtcg gagagagatc
    56101 cagaccgcgg gcgcaaattt cttcaaactc ttcacggtta taagcgatac cgccgccgct
    56161 accgcccatg gtaaaggatg ggcgaataat gcacgggaag cccacgtcag cggcaacagc
    56221 cagcgcttct tccatcgtgt gtgcgatacc ggaacgcgcg gtttccagac caattttctt
    56281 catcgctacg tcgaaacgac ggcggtcttc tgctttatca atcgcatcgg cagtggcacc
    56341 aatcatggtg acaccgaact cttccaacac gccctgacgt tccagctcca gcgcgcagtt
    56401 cagcgccgtc tgaccgccca tggttggcag caccgcgtcc gggcgctctt tttcaataat
    56461 cttgcgtacc acttcccagt gaatcggctc gatgtaggtc gcatcggcca tttccgggtc
    56521 ggtcatgatg gtcgccgggt tggagttcac cagaatgacg cggtaaccct cttcgcgcag
    56581 ggctttacat gcttgcgcgc cagagtagtc aaactcacac gcctgaccga taacaatcgg
    56641 gcccgcaccc agaatcagga tactttttat atctgtacgt tttggcatgg ctcttttact
    56701 cctgattact tagcggtttt acggtactgc tcaattaact tgataaagtg gtcgaacaac
    56761 ggcgcggcgt cgtgtggacc agggctggct tcagggtgac cctggaagct gaacgccggc
    56821 ttatcgatgc gatgaatgcc ctgtaacgta ccgtcgaaca gggatttatg cgtgacacgc
    56881 aggtttgcag gtaatgttgc ttcatccacc gcaaaaccgt ggttctgggc ggtgatcatc
    56941 accacgtttt tctccacatc tttaaccgga tggttgccgc cgtggtgacc aaatttcatt
    57001 ttgacagtct tcgcaccgct cgccagcgcc agcagctgat gaccgagaca gatgccgaat
    57061 accggaatat cggtttcgag gaacttctgg atggcggtaa tggcgtaatc gcacggggcc
    57121 gggtcgccag gaccgttgga gaggaagatg ccgtctggat tcattttcag cacatcttcc
    57181 gcagaagttt gcgccggaac gatggtcagg cgacagcctc tatccaccag catccgcagg
    57241 atgttgcgct tggcaccaaa atcataagcc acgacgtgga acggcagctc gtcttctttt
    57301 ttcgcttctg gcaggccgcc ggtcaacgtc cagctccctt gtgtccagct ataggcttct
    57361 gcggtggtca cttcttttgc cagatccatg ccgttcagac ctgggaacgc gcgggctttt
    57421 tctaacgcca gcgccgcatc cgggttatcg cctgcgataa tgcagccatt ctgtgcgcct
    57481 ttctcgcgca gtaaacgcgt cagcttacgg gtatcgatat cggcaatcgc cacgatgtta
    57541 tggcgcttca ggtaagaaga gaggtcttcg gtattacgga agttgctggc aatcagcggc
    57601 aggtcgcgaa tcaccagacc ttgtgcatgt acctgagaag attcttcatc ggcgtcatta
    57661 gtgccgacat tgccaatatg gggataagta agagtaacga tttgacgaga ataggaagga
    57721 tcagtgagga tttcttgata accggtcatt gaagtattga aaacgacttc cccaaccgcc
    57781 gaacctgttg cccctatggc ccgaccgtga aactgggttc cgtcttccag aaccaatagc
    57841 gctgacttaa tcaaaacacc ctccagagaa tactcactca ctttatttgc atattaattc
    57901 acagtgatga cgtaaatcaa tgcaaatctg cttaccgacg aatttctggc aaacggcggc
    57961 attctggaga tatgggcgct aaaagtcaac ttaatggcag gattttttat attattttgt
    58021 ggctcatgca tgttttacag gcattaagcg acaaaaacat catctaacct gttctggaaa
    58081 acgcttgcgc agcaagaaga ttgcatgaaa taaaaacgag caggaaaaag tggaccaaat
    58141 ggtcaaaatt tacattaaaa caacaaaaat agaagacgaa acatgtaatt tttggcagtt
    58201 atagaaaatt taaaagcata acaccaaaaa taaaagggct aaaaattaaa gccctttata
    58261 attaataaat taaatgtgtt acttttgcat cataataaac attttgtggt tacaaattat
    58321 tgagatcaag cacatctcgc atatcaaaaa gaccactttc cttaccactc aaccacaaag
    58381 ccgatcttac cgcaccgtta gcaaatgtca tgcggctgga cgccttatgg gtgatctcca
    58441 gacgctcgcc aatatcggca aacatcgcgg tatgttcgcc aacgatgtca cctgcacgca
    58501 cggtagcaaa accaatggta cctggcacac gttcaccggt atggccttca cgactgtaga
    58561 ccgcgcaatc tttcagatct ttatcaaggg cgtgggcgat cgcctctccc attgccagtg
    58621 cggtgcctga cggcgcatca actttatgtc tatgatgtgc ttcaataatt tcgatatcgg
    58681 tgtagtcacc catcactttg gctgctttct ccagcagctt aagcatgacg ttaacgccaa
    58741 cgctaaaatt ggcggcaaag acaatcgcaa tatcggcggc ggcgtcacga attgcctgtt
    58801 taccggcttc gtcaaacccc gtagtgccga tcaccatccc tttgccatgc tgccgacaaa
    58861 aagcgagatg gttcagcgta ccttccggac gggtaaaatc gataaacaca tcaaaatcat
    58921 cttttatcgc atcaagactg ctttgcacgg taacgcctgt tttccctgca ccagccagct
    58981 caccggcgtc gctgcccagt aaagaagatc cttcgcgctc cagcgccgcg cccagctgca
    59041 cgccttctaa tgccagtgcc gcctgaatca actggcggcc catacgcccc ccggctcccg
    59101 cgatggcaac gcggatgttt gcatcatgca tagctattct cttttgttaa tttgcataga
    59161 ccgttttcag agtaaccagc gcatcgctac gccgccagcc aaaaacgctg ataattagaa
    59221 attaatgata aacagggagg gatatcagtg aaaggcatga gtcgatgaat gactgcatgc
    59281 cgataacatg tgacaggtta cggcaccaga gccagcacct cagccaccca ctgttggaag
    59341 ccttttacat tcagatccaa tgccacctgt acattggctg gtttgcccag gcaaccgtcg
    59401 atatcaacca ccgtcgtgcc tgaggtaaat tcgccttgag tttctaccgc gacaaaacac
    59461 ggcttgaggg tgaataagtc cgggcgcacc agccaggcga tggcgcagag atcgtgcatt
    59521 cgcagaccgc tttgcatact gccgctacgg tagtggctaa acagggcgtg gagcattttt
    59581 ccggtgcgat tcaactccgg taaagtagcg agatagtcag gggttaatat tgcctgattg
    59641 gtgacatcca gaccgcacat gacaatttcg atgccgctac ggaagacaca ggcagccgct
    59701 tctggatcgg cagcgatatt aaactcggca tttggtgtac agttgccgcg accagcagaa
    59761 ccgcccatga tcaccagacg acgaatatac ggtttacatt ccgggcattg taaaagtaac
    59821 agcgcaatat tggttaacgg gccgatggcc accagggtaa cgggctctgg tgcacgcatc
    59881 agggcatccc gaatcgccag aaacgccggt atcccgagtg gctttcggtt gtgctcaaca
    59941 aagtcgtagc cagccattcc cgattcgccg tgcacagatg ctgcatcacg cggtgcgcgt
    60001 accagcggca cagcggctcc ctgggcgagc ggaatctccg cattccagaa atgcagcagt
    60061 tgcagggcat tgcgggtagt tttctcaacc gagacattac ccgcgacggt ggtcatcagt
    60121 tgcaggtcga gttcgggggc aaaaatcgcg gcggcaatgg cgacggcatc gtcaatgccg
    60181 gggtcagtat cgaggaagat aggtaaacgc atgttttctt cataaaaaat gccggtaaca
    60241 agaccggcat tttttatgaa gaaaacatgc cggtaacaag accggcattt tcgcataact
    60301 taggctgcta atgacttaat cgacttcacg aatatcgaca cgcagctctt tcggcacttc
    60361 gaaaacaata ttttcttcac ggccttccag cggaatggct tcaccaccac ccaactgctg
    60421 caaacgtgcc accacattct gcaccagaat atccggagcc gatgcgcccg cagtcacgcc
    60481 gacgcattta acctctttca cccactcttc ctggatatct ttcgcatcgt caatcaaaaa
    60541 cgcgtgtttg cccatacgct gggccagctc cgccagacgg ttggagttgg aggagttttt
    60601 cgaaccgacc accaacacaa cttccgcctg ttctgccagg gcgcgtaccg cttcctgacg
    60661 gttagtcgtg gcgtagcaga tgtcatcttt gcgcggaccg acaattttcg ggaagcgttt
    60721 acgcagcgcg tcgatcacat cagacgtgtc atccaccgac agcgtggttt gggtcataaa
    60781 ggagagcttc tcttcgtttt tgaccgtcag tttccacaca tcgtctggcg attcgactag
    60841 atacattccc ccttcggggt tgctgtactg ccccatcgtc ccttccactt ccgggtgccc
    60901 ggcgtggccg atgagaatag attcttcgcc acggcgactg gcgcgggcaa cttccatatg
    60961 cactttggtc accagcggac aggtggcatc aaacaccgtc aaatcgcggc ttttcgcttc
    61021 gttacgaacc gcctgagaaa caccgtgtgc ggagaaaatc aggatcgcgc cgtccggcac
    61081 ttcgctaatc tgctcaataa agatagcccc acgctcgcgc aagctatcga ccacgtagcg
    61141 gttatgcacc acttcgtgac ggacatatat cggtgcgccg taaatggcca gcgcgttttc
    61201 aacaatgctg atagcgcggt ctaccccggc acaaaaacca cgcgggttgg ccaacaggat
    61261 ctgcatgtta cgcctccagt gccggatcga tttccagcac ttcaatatca aaatgaacgg
    61321 tctgcccggc cagcggatgg ttgaaatcaa cggtaatgga gtcgccgtta atttcgcgga
    61381 tcacaccagg catctcactg ccatccattg cggtaaaaag catgattgcg ccaatttctg
    61441 gctcgcctgc atccataaat tcacggcggg agaagtactg aatcaggtcc ggtgacggca
    61501 cgccaaacgc cgcatctggc tccagcgaga aggtggtttt gtcgcccact ttcagcccca
    61561 gcaggtgttg ctccagccct tcagaaagag aagcatcgcc caggcggaac agcgccggtt
    61621 taccgttgtt gcgagtagac tcggcggtgg tgccatcgtc gagttttagc gtgaagtgca
    61681 ccaggacggc gctattgctc tgtacagatt cagacatgca ggtttgctct tatttttgag
    61741 gatttttgcc ggatgcggcc tacgatttcg tagcaatctg taggccgcat ccggcaggat
    61801 ttattattgt tttttcgctt tggaaggtaa aaacccttcc agcacgatca gtgccgcacc
    61861 gacacaaatg gcagtatcgg caaggttgaa ggtggcgaag tgccagtcgc cgacgtagaa
    61921 gtcgatcata tcgacaacga agccgtgcca caggcggtcg aacaggttgc ccagcgcgcc
    61981 gccaataatc agcgcgtaag cgatattgtt tagcttctgc gtggccttcg agcgatacat
    62041 catcactgcc aggagcacgc taataccaat cgcaataccg gcaaagaacc aacgctgcca
    62101 gccgccgcta tcggcaagga aactaaacgc cgcgccatag ttacgcgcat aatgcagatt
    62161 aagcgacggg aacagcggga ccgtatcccc cagagcaaag ttctggagga tcaggtattt
    62221 gctgcccaga tcgataatca gcacgactac caccagccac agccagcgta gccctgttga
    62281 acagatcgat tgactcatca ggcaaactta cgtttttcac cgtcaccggc gacgttgctg
    62341 acacagcggc cgcagatttc tgcgtgttcc gccaccttgc cgacatcctg ggtgtagtgc
    62401 cagcagcgtg ggcacttctc accttcggct ttactcaacg cgactttcag ccctttgagt
    62461 acttcgctct gctgagcatc agcaggtgcg tcgttatagt ctgcaacggt agcgccggag
    62521 gtcaacagga caaatcgtaa ttcatcgccc agcgcggtca gtttcgccgc cagttccggt
    62581 tctgcataca aggttactgc cgcttccagc gagccaccca ctttcttgtc ggcacgcgct
    62641 tgctcaatga ctttgttcac ttcgccacgc actttcaaca gctcgtccca gaacgcatcg
    62701 ttcattgctt cactgtctgc cagaccaaac aggccttcgt accactcacc ggtgaagacg
    62761 tatttttcac gttcgcccgg cagatagccc cacacttcat cagcggtgaa ggagaggatt
    62821 ggtgccatcc agcgcaccag cgcttcagcg atgtgataca gcgcagtctg gcagctacga
    62881 cgcgccacgc tatccgcttt ggcggtgtac tgacggtctt tgatgatgtc gaggtagaag
    62941 gaacccatct caacggagca gaagcgcatc agacgctgta ccacttcgtg gaaatcgtat
    63001 gcttcgtacg ccttgaggat gtcttcctgt gccgctttcg cacaacctac ggcccagcga
    63061 tccagtacca ccatctcttc cggtttcacc atatcttttg ctggatcaaa accattcagg
    63121 tttgccagca ggaagcgcgc ggtgttacgg atacgacgat agctatcggc agcacgtttc
    63181 aggatctcgt cagaaacggc catttcaccg gtgtagtcgg ttgatgccac ccacagacgc
    63241 agaatatccg cgcccagttt gttcatcaca tcctgcggcg aaacggtgtt gccgatggat
    63301 ttagacatct tgcgaccctg accatccacg gtaaagccgt gggtcagtac ctgacgatac
    63361 ggcgctttgc ctttcatcgc ggtggagatc attagggaag acatgaacca accacggtgc
    63421 tggtcagaac cttccagata catgtccgct gcgtgaccgg caaattccgg acgcacgtca
    63481 acaacagaag agtgggtaga tccggagtca aaccatacgt cgagagtatc cggcactttc
    63541 acatactggt cagcttcgtc gccgaggatc tctttcgcat cgagatccca ccacgcctgg
    63601 atgccgtcaa cttcaacgcg tttagccact tcttccatca gttcgagggt acgcggatgc
    63661 agctcttccg tgtctttgtg cacgaacagt gacatcggta cgccccaggt gcgctgacgg
    63721 gagatacacc agtcaggacg gttagcaacc atcgactcga tacgcgcctg gccccagtcc
    63781 gggagccact gcacgccttt gatctctttc agtgactgcg cacgcagacc tttctgatcc
    63841 atgctgacga accactgcgg tgtcgcgcgg aagatgatcg gcgttttgtg acgccagcag
    63901 cacggatagc tgtgctgcat tttctcaacg tgcagcagcg cgcctttttc ctgcagcagc
    63961 gcaacgacga tgtcgttcgc tttgaagacg ttcacgccat ccagcgtcgg ataagtgccc
    64021 ggcagataag tgccgtccgg gccaaccggg ttagcggttt ccaggccgta tttctgaccg
    64081 atcacatagt cgtccgggcc gtggccaggc gcggtgtgaa cggcaccggt accggcatcc
    64141 agggtaacgt gatcgccgag gattgccgga acgtcgaagc ccataaacgg atgggtaaag
    64201 cgcagcagct caagctctgc accttttacc gtgccgagaa tggtgtaatc ggtcacgccg
    64261 atacgctgca ttacgctttc aaccagatct ttcgccagaa tcacggcctg accgtcgatc
    64321 tgcaccagcg catagtcgaa atcaggtgca atagagattg cgcggttcgc aggcagagtc
    64381 cacggcgtag tggtccagat caccagcgag attgggccgt taacgttgct tacgccaaat
    64441 ttcgctttca gcgcatcctg atcgaccgcc tggaaagcga cgtcaatgga cggagaagtt
    64501 ttgtcgtaat actcaacttc cgcttctgcc agtgcagaac ggcagtctac gcaccagtgc
    64561 accggcttcg cgcctttgtg caggtgacca ttgccgatga ttttgcccag cgcgcggatg
    64621 atgttggctt cagttttgaa gtccatagtc agatacgggt gcgaccagtc gcccagcacg
    64681 cccagacgga taaagtcttt gcgttgaccg tcaacctggg tcgccgcgta ttcgcggcac
    64741 ttggcgcgga actcggcggc ggtgaatttc tcacccggct taccgtattc ttgctctact
    64801 ttcagctcga tcggcagacc gtggcagtcc cagccaggca catacggcga gtcataaccg
    64861 gaaagccctt tggacttcac gataatgtct ttcagaatct tgttaaccga gtgaccaata
    64921 tgaatgctgc cattcgcata aggagggcca tcatgcagaa tgaaggtttt tttgcctttt
    64981 ttagccgcac ggatgatgcc gtacagatca tcatcagtcc aacgcgccag cattcccggt
    65041 tcgcgcttgg cgagatcgcc acgcatcggg aaccctgttt ccggcaaatt cagggttgat
    65101 ttatagtcac tcatcagatt ctcggttccg tgtttcggtt tgattacata acaggcttaa
    65161 gccggttttg ttagcccaaa aaattcgcgg gcggttaatt catcacgcgc aatctgcgct
    65221 ttcagttcgt ccagcgacgc aaatcgctgc tcattgcgta tttttttacg cagcactact
    65281 tgtatatggc gaccgtaaag gtccattgca acatctaaca aatgcacttc cagctgctgg
    65341 cgaataccgg caaccgttgg gcgtgttccg atgtttgcca cgccgggtaa cggcttttca
    65401 ccgaggccca gcacttctac cgcataaacc cctttcaccg gggaaacctg acggcgtagc
    65461 ggtacattcg ccgtcgggaa acctatagtg cgccctaatt catcaccgtg gactacacgc
    65521 ccggagatgg caaacgggtg ccccagtaaa ctttctgcca gagccagatt gtcatcagcc
    65581 agcgcctgac gcacggcggt gctgctgata cgcacaccac cttcgcaaaa agtttgcgta
    65641 ctggtgatat cgaagccgta ttccatgcca gctttctgta ataacaagaa atcgccttca
    65701 cgaccagcgc caaagcggaa atcatcacct acggcaagaa attttacgcg caaatgcttc
    65761 accaaaagat cgctgatgaa attttgcgcg gttaacgccg caaaacgcct gtcgaaacgc
    65821 acgcacagca cgtaatcaac gccacactct gcaaggtaac gcagtttttc ccgcagccgg
    65881 gtcagtcttg ccggggcttt atcggtagca aacagttcca gtggttgagg ttcaaaaagc
    65941 atcaccatca ccggtaagtt gcgcttgcgc ccttcttcct gcaagccctg taacagcgcg
    66001 cgatgaccgc gatgcacgcc gtcgaaatta ccaatagtca gcacacaccc ttcttgcggg
    66061 gcctggctga gattatgtat gccgcgtatc agcttcatgt ctggctcaaa acagtgaaaa
    66121 tcgtccgagt ataccttgta cagcggtcaa ggttaaccgg cgattgagta ccgaaatcag
    66181 aaagaagtgg cttttcattg ccatggagca aatcacggga agaaactgac cgcctgctgc
    66241 aatttttatc gcggaaaagc tgtattcaca ccccgcaagc tggtagaatc ctgcgccatc
    66301 actacgtaac gagtgccggc acattaacgg cgcttatttg cacaaatcca ttgacaaaag
    66361 aaggctaaaa gggcatattc ctcggccttt gaattgtcca tatagaacac atttgggagt
    66421 tggaccttgg ctaatatcaa atcagctaag aagcgcgcca ttcagtctga aaaggctcgt
    66481 aagcacaacg caagccgtcg ctctatgatg cgtactttca tcaagaaagt atacgcagct
    66541 atcgaagctg gcgacaaagc tgctgcacag aaagcattta acgaaatgca accgatcgtg
    66601 gaccgtcagg ctgctaaagg tctgatccac aaaaacaaag ctgcacgtca taaggctaac
    66661 ctgactgcac agatcaacaa actggcttaa tcgccaattt gctgaagctt tgtgaaaaag
    66721 cccgcgcaag cgggtttttt tatgcctgct gcttttgtga tgcgtgagag aacacactgc
    66781 cggttgccag atagtcaaaa gagacatgcc gttgtactat ctgaactctt cgtggcttaa
    66841 gaatcgtaga accatcggct gatacattct gaacaagaat atctactcaa tctccagact
    66901 cccccacaaa gaatatggat attgtgatac acattgaggt attaaactta agaccactct
    66961 atttttgaat taaattccta tatactttaa ccattcataa atatctcctc attagacctt
//
LOCUS       DAJTUI010000009        66662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTUI010000009 DAJTUI010000000
VERSION     DAJTUI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.009"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO464_000643"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO464_000643"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6225905.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO464_000644"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO464_000644"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6225906.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO464_000645"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO464_000645"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6225907.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO464_000646"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO464_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6225908.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO464_000647"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO464_000647"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6225909.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO464_000648"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO464_000648"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6225910.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="OO464_000649"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="OO464_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6225911.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO464_000650"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO464_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6225912.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO464_000651"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO464_000651"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6225913.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO464_000652"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO464_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6225914.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO464_000653"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO464_000653"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6225915.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="OO464_000654"
     CDS             complement(15665..16702)
                     /locus_tag="OO464_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6225916.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="OO464_000655"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="OO464_000655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6225917.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="OO464_000656"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="OO464_000656"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR6225918.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="OO464_000657"
     CDS             19444..19884
                     /locus_tag="OO464_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR6225919.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="OO464_000658"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="OO464_000658"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6225920.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21601)
                     /locus_tag="OO464_000659"
                     /pseudo
     CDS             complement(20611..21601)
                     /locus_tag="OO464_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="OO464_000660"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="OO464_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6225921.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="OO464_000661"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="OO464_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR6225922.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="OO464_000662"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="OO464_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR6225923.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="OO464_000663"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="OO464_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6225924.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="OO464_000664"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="OO464_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6225925.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="OO464_000665"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="OO464_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6225926.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="OO464_000666"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="OO464_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6225927.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLTLTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="OO464_000667"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="OO464_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6225928.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="OO464_000668"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="OO464_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6225929.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="OO464_000669"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="OO464_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6225930.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="OO464_000670"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="OO464_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6225931.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="OO464_000671"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="OO464_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6225932.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="OO464_000672"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="OO464_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6225933.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36697)
                     /gene="ftsY"
                     /locus_tag="OO464_000673"
     CDS             complement(35180..36697)
                     /gene="ftsY"
                     /locus_tag="OO464_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6225934.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEEAAKEEEAAKEEITDE
                     ELEAQALAAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKCSLLKTKENL
                     GSGFISLFRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALY
                     GLLKEEMGEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVML
                     AAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTA
                     GRLQNKSHLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTG
                     ITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36847..37443
                     /gene="rsmD"
                     /locus_tag="OO464_000674"
     CDS             36847..37443
                     /gene="rsmD"
                     /locus_tag="OO464_000674"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6225935.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37433..37702
                     /locus_tag="OO464_000675"
     CDS             37433..37702
                     /locus_tag="OO464_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6225936.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37705..38064)
                     /locus_tag="OO464_000676"
     CDS             complement(37705..38064)
                     /locus_tag="OO464_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR6225937.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38205..38831
                     /locus_tag="OO464_000677"
     CDS             38205..38831
                     /locus_tag="OO464_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6225938.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38905..41103
                     /gene="zntA"
                     /locus_tag="OO464_000678"
     CDS             38905..41103
                     /gene="zntA"
                     /locus_tag="OO464_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6225939.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41205..41450)
                     /gene="tusA"
                     /locus_tag="OO464_000679"
     CDS             complement(41205..41450)
                     /gene="tusA"
                     /locus_tag="OO464_000679"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6225940.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41671..42336
                     /locus_tag="OO464_000680"
     CDS             41671..42336
                     /locus_tag="OO464_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6225941.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42409..42966
                     /gene="dcrB"
                     /locus_tag="OO464_000681"
     CDS             42409..42966
                     /gene="dcrB"
                     /locus_tag="OO464_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6225942.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42970..44187)
                     /locus_tag="OO464_000682"
     CDS             complement(42970..44187)
                     /locus_tag="OO464_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6225943.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44321..45370
                     /locus_tag="OO464_000683"
     CDS             44321..45370
                     /locus_tag="OO464_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6225944.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45425..46012
                     /gene="acpT"
                     /locus_tag="OO464_000684"
     CDS             45425..46012
                     /gene="acpT"
                     /locus_tag="OO464_000684"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6225945.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46123..47697
                     /gene="nikA"
                     /locus_tag="OO464_000685"
     CDS             46123..47697
                     /gene="nikA"
                     /locus_tag="OO464_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6225946.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47697..48641
                     /gene="nikB"
                     /locus_tag="OO464_000686"
     CDS             47697..48641
                     /gene="nikB"
                     /locus_tag="OO464_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6225947.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48638..49471
                     /gene="nikC"
                     /locus_tag="OO464_000687"
     CDS             48638..49471
                     /gene="nikC"
                     /locus_tag="OO464_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6225948.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49471..50235
                     /gene="nikD"
                     /locus_tag="OO464_000688"
     CDS             49471..50235
                     /gene="nikD"
                     /locus_tag="OO464_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6225949.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50232..51038
                     /gene="nikE"
                     /locus_tag="OO464_000689"
     CDS             50232..51038
                     /gene="nikE"
                     /locus_tag="OO464_000689"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6225950.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51044..51445
                     /gene="nikR"
                     /locus_tag="OO464_000690"
     CDS             51044..51445
                     /gene="nikR"
                     /locus_tag="OO464_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6225951.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51565..51924)
                     /locus_tag="OO464_000691"
     CDS             complement(51565..51924)
                     /locus_tag="OO464_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6225952.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51921..52196)
                     /locus_tag="OO464_000692"
     CDS             complement(51921..52196)
                     /locus_tag="OO464_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR6225953.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52255..53379)
                     /locus_tag="OO464_000693"
     CDS             complement(52255..53379)
                     /locus_tag="OO464_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6225954.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53379..56114)
                     /gene="rbbA"
                     /locus_tag="OO464_000694"
     CDS             complement(53379..56114)
                     /gene="rbbA"
                     /locus_tag="OO464_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6225955.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56111..57178)
                     /locus_tag="OO464_000695"
     CDS             complement(56111..57178)
                     /locus_tag="OO464_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6225956.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57544..59166)
                     /locus_tag="OO464_000696"
     CDS             complement(57544..59166)
                     /locus_tag="OO464_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6225957.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59428..61017)
                     /locus_tag="OO464_000697"
     CDS             complement(59428..61017)
                     /locus_tag="OO464_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6225958.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61401..62432
                     /locus_tag="OO464_000698"
                     /pseudo
     CDS             61401..62432
                     /locus_tag="OO464_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62519..62995)
                     /locus_tag="OO464_000699"
     CDS             complement(62519..62995)
                     /locus_tag="OO464_000699"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225959.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62988..63752)
                     /locus_tag="OO464_000700"
     CDS             complement(62988..63752)
                     /locus_tag="OO464_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6225960.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63998..64759
                     /locus_tag="OO464_000701"
                     /pseudo
     CDS             63998..64759
                     /locus_tag="OO464_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(64866..65366)
                     /locus_tag="OO464_000702"
     CDS             complement(64866..65366)
                     /locus_tag="OO464_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225961.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65517..>66662)
                     /locus_tag="OO464_000703"
     CDS             complement(65517..>66662)
                     /locus_tag="OO464_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6225962.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16192 a        17894 c        16347 g        16229 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ttggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacag cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcagcacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc gtgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg aagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggccagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaaaatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgtcagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcggg aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag cccttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat cagattaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aacagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcatttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttctttagcc gcttcttctt cttctgccgc ttcgacaatc tctacggttt
    36301 ccgcttcagc ctgccactct tctgacgaaa cctcttcggc gttgacgtct tccggcaacg
    36361 gcagctcttc acgttcgata gccactggct ccggcgtttc ttctacgacc ggttccggct
    36421 gtgcaacaac ttccgcttca ggctgcgctt tttcactttc aacaacctgt tcagtgactt
    36481 ccacaacgtc ggcagcaaaa gtttccgcct cggcttcagt atgcgcctgc ggctgctctt
    36541 caacggcttg ttcagaagcc ttcgcaggct cttgcgcctg aacgatttct tctacaaccg
    36601 gttgttcatt ctgaacttct gtctcttttt ccggggtctg ctctttttga ccaaagccca
    36661 gccaggaaaa aaagccacgt cttttttctt tcgccatttg cgactacact cctcgctgtt
    36721 gcttcatggc acagcgttaa cgctatgtac atagcagcta aaaaaatgat gaaatagtct
    36781 atcacttaac ttaattcaca tcaccgcctg caagtatgtg ttatctggcg gattgagcaa
    36841 tttatcatga aaaaaccgaa tcattccggc agcggccaaa tccgcattat tggcgggcag
    36901 tggcgaggcc gtaaactccc ggttcctgat agcccaggtc tgcgccccac caccgaccgc
    36961 gtacgcgaaa cgttgtttaa ctggctggct ccggtcattg ttgacgccca atgtctggat
    37021 tgcttcgccg ggagcggcgc gctggggctg gaagcgttat cgcgctacgc tgcgggggca
    37081 acgttgattg agatggatcg cgtggtttct cagcagttaa ttaagaatct ggcgacacta
    37141 aaagcaggca atgcacgcgt ggtgaacagc aacgcaatgt cattcctggc gcaaaaaggc
    37201 acaccgcata atatcgtgtt tgtcgatcca ccgttccgcc gtggcttgtt agaagagacg
    37261 ataaatttac tggaagataa cggctggctg gctgacgaag ccctgattta tgtcgaaagc
    37321 gaagtcgaaa acggtctgcc cactgttccg gcaaactggt cattacatcg ggaaaaagtg
    37381 gcgggtcagg tggcttatcg gctgtatcaa cacgaagcac aaggagaaag tgatgctgat
    37441 taatattggt cgtttgttaa tgctctgcgt ttggggattt ttaatcctca atctggtgca
    37501 tcccttcccg cgcccgctaa atattttcgt taacgtggcg ctgattttta ccgtgctgat
    37561 gcatggtatg cagctggcgc tattgaaatc cactttaccg aaagatggcc cgcagatgac
    37621 caccgccgaa aaggtgcgga ttttcctttt cggcgtgttt gaactgctgg tctggcagaa
    37681 gaaatttaaa gttaaaaaat aaccttattg ttcgccaaca aagctaacaa agcgcgttcc
    37741 tttatagctc agcgtacctt tatcgcccac tgtcagggcg tggtactgct gggcgtcgag
    37801 gcgaaacgtc tgctccattc ctccgctttg cggtttgaag cttgcctcat agcgcatact
    37861 ggtgcctgcc ggagtcactt cctgctggcg cgaacggcga tcgttaatcg gtttttcccg
    37921 cttgttgctc accaccacca gcttttgctg gagcggagcc atatcattat cagctttttc
    37981 ccgtcgctgt tgcataaaac gaaacgatgc ggcgacgaca attaagccaa tgataacaat
    38041 aaagaaaaga ggtggtttac tcatctttat ccctcatcgg aaaatgcgga attaagcata
    38101 ccctgccagt tatggtgttg tcatccgtcc accctcgcca ctaaactgga agcaagaccg
    38161 taggcattct gcttacgaaa aaataacgaa ttcaaggaac taagatgctt tggtcgttta
    38221 tcgctgtctg tctttccgca tggctatctg tggatgcatc gtatcgtggg ccaacctggc
    38281 aacgctgggt gtttaaaccg ttaacccttc ttctcctgct gttactggcc tggcaagcac
    38341 cgatgttcga cgccattagc tatctggtgc tggcgggact gtgcgcctca ctgctgggcg
    38401 atgcgctaac cctgttgcca cgtcaacgtc tgatgtacgc catcggcgcg tttttcctct
    38461 cgcacctgct gtacaccatc tatttcgcca gtcagatgac gctctctttc ttctggcctc
    38521 tgccactggt gttgctggtt ctgggtgcgc tgctactggc gattatctgg acgcggctgg
    38581 aagagtaccg ttggcctatc tgcacgttta tcggcatgac gctggtgatg gtgtggctgg
    38641 caggtgaact gtggttcttc cgtccgaccg ctccggcgct ctctgcgttt gttggcgctt
    38701 cgttgctgtt tatcagtaac tttgtctggc tggggagcca ctatcgccga cgcttccgtg
    38761 cggataacgc gattgctgcg gcctgctact ttgccggtca cttcctcatc gtccgctcac
    38821 tgtatctctg ataaaacttg actctggagt cgactccaga gtgtatcctt cggttaatga
    38881 gaaaaaactt aaccggagga tgccatgtcg acacctgaca atcacggcaa gaaagcccct
    38941 caatttgctg cgttcaaacc gctaaccacg gtacagaacg ccaacgactg ttgctgcgac
    39001 ggcgcatgct ccagctcgcc aacgctctct gaaaacgtct ccggcacccg ctatagctgg
    39061 aaagtcagcg gcatggactg cgccgcctgt gcgcgcaagg tagaaaatgc cgtgcgccag
    39121 cttgcaggcg tgaatcaggt gcaggtgttg ttcgccaccg aaaaactggt ggttgatgcc
    39181 gacaatgaca tccgtgccca ggttgaatct gcggtgcaaa aagcgggcta ttccctgcgc
    39241 gatgagcagg ccgccgacga gccacaagca tcgcgcctga aagagaatct gccgctgatt
    39301 acgcttatcg tgatgatggc aatcagctgg ggtctggagc agttcaatca tccgttcggg
    39361 caactggcgt ttatcgcgac cacgctagtt gggctgtacc cgattgctcg tcaggcatta
    39421 cggctgatca aatccggcag ctacttcgcc attgaaacct taatgagcgt agccgctatt
    39481 ggtgcactgt ttattggcgc aacggctgaa gctgcgatgg tgttgctgct gtttttgatt
    39541 ggtgaacgac tggaaggctg ggccgccagc cgcgcgcgtc agggcgttag cgcgttaatg
    39601 gcgctgaaac cagaaaccgc cacgcgcctg cgtaacggtg agcgggaaga ggtggcgatt
    39661 aacagcctgc ggcctggcga tgtgattgaa gtcgccgcag gtgggcgttt gcctgccgac
    39721 ggtaaactgc tctcaccgtt tgccagtttt gatgaaagcg ccctaaccgg agaatccatt
    39781 ccggtggagc gcgcgacggg cgataaagtt cctgcaggag ccaccagcgt agaccgtctg
    39841 gtaacgctgg aagtgctgtc agaaccgggt gccagcgcca ttgaccggat tctgaaactg
    39901 atcgaagaag ccgaagagcg ccgcgccccc attgagcggt ttatcgaccg tttcagccgt
    39961 atttatacgc cagcgattat ggccgtcgcc ctgctggtaa cgctagtgcc gccgctgctg
    40021 tttgccgcca gctggcagga gtggatttat aaaggtctga cgctgctgct gattggttgc
    40081 ccgtgtgcgt tagttatctc aacgcctgcg gcgataacct ccgggctggc agcagcagcg
    40141 cgtcgtgggg cgttaattaa aggcggtgcg gcgctggaac aactaggtcg tgttacccaa
    40201 gtggcgtttg ataaaaccgg tacgctgacc gtcggtaaac cgcgcgttac cgcgattcat
    40261 ccggcaacgg gtattagtga atctgaactg ctgacactgg cggcggcggt cgagcaaggc
    40321 gcgacgcatc cactggcgca agccatcgta cgcgaagcac aggttgctga actcgccatt
    40381 cccaccgccg aatcacagcg ggcactggtc gggtctggca ttgaagcgca ggttaatggt
    40441 gagcgcgtgt tgatatgcgc tgccgggaaa catcccgctg atgcatttgc tggtttgatt
    40501 aatgaactgg aaagcgccgg gcaaacggtt gtgctggtag tacgtaatga tgacgtgctg
    40561 ggtatcattg cattgcagga taccctgcgc gccgacgcta caactgccat cagtgaactg
    40621 aacgcgctgg gggttaaagg agtgatcctc accggcgata atccacgcgc agcggcggca
    40681 attgccgggg agctggggct ggagtttaaa gcgggcctat tgccggaaga taaagtcaaa
    40741 gcggtgacca agctgaatca acatgcgccc ctggcgatgg tcggtgatgg tattaacgac
    40801 gcgccagcga tgaaagctgc cgccatcggg attgctatgg gtagcggcac agacgtggcg
    40861 ctggaaaccg ccgacgcagc attaacccat aaccacctgc gcggcctggt gcaaatgatt
    40921 gaactggcac gcgccactca cgccaatatc cgccagaaca tcaccattgc gctgggactg
    40981 aaagggatct tcctcgtcac cacgctttta gggatgaccg ggttgtggct ggcagtgctg
    41041 gcagatacgg gtgcgacggt gctggtgacg gcgaatgcgt taaggttgtt gcgcaggaga
    41101 taaggcaaac cgatcgcaac attgagcgcg atcggtcccc tcgcccctct ggggagaggg
    41161 ttagggggtg aggaaaaggc ggcatcgaag ccaatcagcc cctatcaacc gcctttacga
    41221 atcaaataac gataaggcag tccatccgtc tctttcgcaa ccagttcgtg ttccataaag
    41281 gtacaaaacc caggaatatc gcgggtagtg gccggatcgt cggcgataat cagcaacgtt
    41341 tcgccaggct gcatattgcg cacggttttg cgcaccatca tcaccggttc cgggcagcgc
    41401 aggccaagcg cgtcgagtgt gtggtcaggg ctggaaaaga gatcggtcat tttcttctca
    41461 tcacttaaaa aaacggcgct agtttacgcc ctgtgagtcc gtaagcaaac caggttaacg
    41521 attgcgtgaa aattaaccat tgcattgtca acgtaaagca gtatcatgcg gcggctcgaa
    41581 aaaagggtaa gcacgttatt atgttaaggt aacagacgtg tcgtacgtat tgggttccct
    41641 caccccaatg gttaatcaaa aaggtacaat atgaacgttt tctcgcaaac tcaacgctat
    41701 aaggcgttgt tctggttatc gttatttcat cttctggtga tcacctccag taactatctg
    41761 gttcagcttc ccgtctccat ttttggtttc cataccacct ggggcgcgtt tagctttccg
    41821 tttatttttc ttgctaccga cctgaccgtg cgtatttttg gcgcaccgct ggcccgacgc
    41881 attatcttcg cggtaatgat ccctgcgtta ttaatctcct acgtcatctc gtcgctattc
    41941 tatatgggtt cctggcaggg attcggcgca ctcgcccact tcaacctgtt tgtcgcccgt
    42001 atcgccaccg ccagtttcat ggcctacgcg ctggggcaaa tcctcgacgt gcacgttttt
    42061 aaccgcctgc gtcagagtcg ccgctggtgg ctggcaccga cagcgtccac actgttcggt
    42121 aacgtcagcg acacgctggc ctttttcttc attgccttct ggcgtagccc ggatgccttt
    42181 atggctgaac actggatgga aatcgcgctg gtcgattact gtttcaaagt gttaatcagt
    42241 atcgttttct tcctgccaat gtatggcgta ttactcaata tgctgttgaa aagactggca
    42301 gataaatccg aaatcaacgc tttgcaggcg agttaaaggt tcgttatcag agttgtgata
    42361 agatggatga atgagccgtt atggccgttt atcgaaagga agaagtcaat gcgcaatctg
    42421 gttaaatatg tcggaattgg cctgctggtt atggggcttg cggcctgtga tgataaagac
    42481 actaacgcta cggcgcaggg ttcggttgcg gaaagtaacg ctaccgggaa tcccgtcaac
    42541 ctgcttgatg gcaagttaag tttctcgctg ccagcggata tgaccgacca gagcggtaag
    42601 ctgggaacgc aggccaataa catgcatgtc tggtccgacg ccaccgggca gaaagcagtc
    42661 atcgtcatca tgggcgatga tccgaaagaa gatctggcgg tgttggcgaa gcgtctggaa
    42721 gatcagcaac gtagccgcga tccgcagctg caagtggtaa ccaataaagc cattgagctg
    42781 aaaggtcaca aaatgcagca gttagacagt attatctccg cgaaaggcca gacggcgtac
    42841 tcttccgtta ttctgggtaa cgtgggtaat caactgctga ccatgcaaat tacgctgcca
    42901 gctgacgatc agcaaaaagc gcaaaccacc gcagaaaaca tcattaatac gctggttatt
    42961 cagtaagttt taagatgatg aggcggcctc agggacgtgt tccggaggcc gtttttttaa
    43021 tcgccacgtc agtaataacg cgattgcgac cagtcccgcc gccgccagat aaatcaccgg
    43081 tacgcccgcc cagctcatca ccagcccagc cagtggtcca gtcacgccaa gcgataaatc
    43141 cataaatacg gtgtaagttg ccagcgccgc cccctgattt tgctgcggaa ccgcttttac
    43201 cgccactaca cccaatgccg ggaacaccag cgaaaacccg gcccccgcca gtaagacgcc
    43261 gattttcgcc atccacggca tagtcgccac gccaaccagt agcaggccga ttatctcaac
    43321 gctaaagcaa atcatcgcta cgtttaagcc accgatacgg ttaatgccgt tagggaataa
    43381 caaacgcgta ccgacaaacg cacagctaaa cagcgtcagc gcgaaagccg caccgtccca
    43441 acctttagcg tcataaaaca gcgtgataaa ggtggcgatg acgccaaatc cggcggaagc
    43501 cagtgccagc gccataccgt acagccagac gcgcccaagc accgcgcgaa acggcagcgg
    43561 tttgccttta ctggctttta ccgtcggacg cgggatcgcc aacaaaatgg ccaccagcgc
    43621 cacgcccata atgattaacg ctaacgcctg caagccgccc cagtgataaa acacgacgcc
    43681 taacggcgca cccatcgcca tcgccccgta agtgacaatg ccgttccacg aaatcacccg
    43741 cccgatatgc agcgagccaa ccactccaac gccccatagg gtcgatcccg ttccggcaaa
    43801 actttgccca atcccaagga tgacgcgccc caggcaaagt aataacaggc tgatgacagg
    43861 cagactggcg gttaatcctg ccgtcagata ccccagaccg ctcaaaaagc agccgcataa
    43921 accgaagacg acaatctttt tgggtcccag caaatcggcg taacgtccgg catgagggcg
    43981 gctcagcaag gtggcgaaat attgcaggct gataaccaat cctgcccaga aggcgctaaa
    44041 gcccatcaca tcatggacat agcccggtaa tacagcgagc ggcaacccga tggtgaggta
    44101 gctggcgaag ttaaacatga caatggagac aatgcgcaaa ttcaggcgca atccgtttag
    44161 cgcgggttcg gctacgggtt cgggcatgag gatcaccaca tttttacaac agtgtttcat
    44221 ttttaccatg tgctgacgtg aaaatcagca gtaagaatca gaatattgct ggcgtgactc
    44281 ccgcgctaca cttaacacaa aaagtcacaa ggaagcccca atggaaaccc ctcaacccga
    44341 taaaacgggc atgcacattc tgctcaagtt ggcctcgctg gtagtgatcc tcgcgggcat
    44401 tcacgcagcg gcagatatca ttgtgcagct gttactggcg ctgttttttg ccatcgtcct
    44461 caacccgctc gttacctggt ttattcgtcg gggagtacaa cgccccgttg ccattacgat
    44521 tgtagtggtg gtgatgctga tcgcactaac cgcgctggtc ggcgtactgg cggcatcgtt
    44581 taacgaattt atctctatgc tgccgaagtt taataaggag ctgacgcgca aactttttaa
    44641 attgcaggag atgttgcctt ttcttaattt gcatatgtcg ccggagcgaa tgctgcagcg
    44701 gatggactcg gaaaaagtgg ttaccttcac cacagcgcta atgaccgggc tttccggggc
    44761 aatggcgagc gtgcttttgc tggtgatgac cgtagttttt atgctgtttg aagtgcgcca
    44821 cgtcccttac aaaatgcgtt ttgcgctgaa taacccacag attcacatcg caggattaca
    44881 ccgcgcactt aaaggcgttt cgcattatct ggcgttaaag acgctactca gtttatggac
    44941 aggtgtcatc gtctggctgg ggctggcgct aatgggcgta cagtttgcgc tgatgtgggc
    45001 agtactggcg tttttgctca actacgtgcc caatatcggc gcggtaattt ccgccgtacc
    45061 gccaatgatt caggtgctgc tgtttaatgg tgtttacgaa tgtattctgg tcggcgcatt
    45121 gtttttagtg gtccatatgg tcatcggcaa tattttagaa ccacggatga tgggccatcg
    45181 cctggggatg tccaccatgg tggtatttct ttcattgtta atctggggat ggctgctcgg
    45241 cccggtaggg atgctacttt cggtgccatt aaccagcgtg tgtaaaatct ggatggaaac
    45301 gacgaaaggt ggcagcaaac tggcgatttt actggggccg ggcagaccga aaagtcggtt
    45361 accgggatga ggcgacaagt gatacgatac gcactttcat tttccattaa acgttggccc
    45421 tgatatgtat cggatagttc tggggaaagt ttcgacctta agcgcagctc cactgccacc
    45481 gggtttacgc gagcaagcac cgcaaggtcc acgacgcgaa cgctggctgg cggggcgtac
    45541 attgctttcg cacacgcttt ccccgctacc ggagatcatc tatggcgaac aaggcaaacc
    45601 tgcattcgcg ccggaaacgc cgctatggtt caacttaagc catagcggtg acgatatcgc
    45661 tctgctgttg agcgatgaag gcgaagtcgg ctgcgatatc gaagtgattc gcccgcgcgt
    45721 caactggcgc tggctggcga acgccgtgtt cagcctcggg gaacacgctg aaatggacgc
    45781 cgtgcatcct gatcagcaac tggaaatgtt ctggcgcatc tggacgcgca aagaagccat
    45841 cgttaaacag cgtggcggca gcgcctggca aatcgtcagc gtagatagca cctatcactc
    45901 ctcgctgtca gtcagccatt gtcagctaga aaatttaagc cttgcgatct gcacccccac
    45961 cccctttacg ctcaccgccg acagtgtgca atggatcgat tcagttaact gatccgccca
    46021 cccgactgcc catctattga tccagaacag gtaatcagta tgacgaatac ttaaaatcgt
    46081 catacttatt tccgccatct attttaatcc attggggtta ccatgctctc cacactccgc
    46141 cgcactctat ttgcgctgct ggcttgtgcg tcttttatcg tccatgccgc tgcaccagat
    46201 gaaatcacca ccgcctggcc ggtgaatgtc gggccactaa acccgcacct ttacacgcct
    46261 aaccagatgt tcgcccagag catggtttat gaaccattgg tgaaatatca ggcagacggt
    46321 tcggtgatcc cgtggctggc aaaaagctgg actcattcag aagatggtaa aacctggacc
    46381 ttcaccctgc gtgatgacgt gaaattctcc aacggtgaac cgttcgatgc cgaggcggcg
    46441 gcagaaaact tccgcgcagt gctcgataac cgtcaacgtc acgcctggct ggagctggca
    46501 aaccagattg ttgatgttaa agcactcaat aaaacagagc tgcaaattac cctgaaaagc
    46561 gcctactatc ctttcctgca agaactggcc ctgccccgtc ctttccgttt tatcgctccc
    46621 tcgcagttta aaaaccatga aaccatgaac ggaattaaag cgccgattgg caccggaccg
    46681 tggattttgc aggaatcgaa actaaatcag tacgatgtct tcgtccgtaa cgaaaactac
    46741 tggggcgaaa agccagcgat taaaaagatc acctttaacg tcatcccgga cccgactacc
    46801 cgcgcggtgg cgtttgaaac tggcgatatc gacctgctgt acggaaacga agggttatta
    46861 ccgctcgata ccttcgcccg ctttagccag aatccggctt accacaccca actgtcacag
    46921 ccgatcgaaa ccgtgatgct ggcgctcaat accgccaaag cccccaccaa cgagctggca
    46981 gtacgtgaag ctcttaatta cgcggtaaac aaaaaatcgc tgattgataa cgcgttatat
    47041 ggcacccagc aggtcgccga caccctgttt gccccttctg tgccttacgc taatctcggc
    47101 ctgaaaccgc gccagtacga tccgcagaaa gcgaaagagt tgctggaaaa agccggttgg
    47161 acgctgcctg cgggcaaaga catccgcgag aaaaatggtc agccgctgcg cattgaactg
    47221 tcgttcatcg gcaccgatgc gttaagcaaa tcgatggcgg aaatcattca ggctgatatg
    47281 cgccagattg gcgcagatgt ctcgctgatt ggcgaagaag agagcagtat ctatgctcgt
    47341 cagcgcgacg gtcgttttgg catgattttc caccgcacct ggggcgcgcc atacgatcca
    47401 cacgccttcc tgagttcaat gcgcgtaccg tcgcacgctg acttccaggc acagcaagga
    47461 ttagccgaca agccgctgat tgataaagag atcggcgaag tgctggcgac ccatgacgaa
    47521 acgcaacgtc aggcgctgta tcgcgacatt ctgacccgtc tgcatgacga ggcggtttat
    47581 ctgcctatca gctacatctc gatgatggtg gtatcaaaac cggagctggg taatatcccc
    47641 tacgcgccga tcgccaccga aattccgttc gaacagatta aaccggtgaa accttaatgt
    47701 tgcgttacgt attacgccgc tttctgctgc tgatcccgat ggtgctcgcc gcctcggtga
    47761 tcatttttct gatgctgcgc ctcggtaccg gcgacccggc gctcgattat ctgcgtttat
    47821 ctaacctgcc gccgacgccg gagatgctgg cctctacccg caccatgctc gggctggatc
    47881 agccgctgta cgtccagtac ggtacctggt tgtggaaggc gctgcatctt gactttggta
    47941 tctcattcgc cagccaacgc ccggtactgg acgatatgct gaacttcctg cccgccacgc
    48001 tggaacttgc aggtgcggcg ctggtattaa ttctgctcac ttccgtaccg ctcggtatct
    48061 gggcggcgcg ccaccgcgat cgtttgccgg atttcgccgt gcgctttatc gcgtttcttg
    48121 gcgtgtcgat gcccaacttc tggctggcat ttttgctagt gatggcgttt tcggtgtatc
    48181 tgcaatggct acccgcgatg ggctacggca gctggcagca catcatttta cctgcggttt
    48241 ccatcgcctt tatgtcgctg gcgattaacg cgcgactgct gcgcgccagt atgctggacg
    48301 tcgccggtca gcgtcacgtc acctgggcgc ggctacgcgg cctgaatgac aaacagaccg
    48361 aacgccgcca tattctgcgc aatgcctcgc tgccgatgat caccgccgtg gggatgcaca
    48421 ttggcgaatt gattggcggg acgatgatta tcgaaaacat ctttgcctgg cctggcgtcg
    48481 ggcgctatgc ggtgtcggcg atttttaacc gtgactatcc ggtgattcag tgctttacgc
    48541 tgatgatggt ggtggttttt gtggtctgta atttgattgt cgatttgctc aacgccgcgc
    48601 tggacccgcg cattcgtcgt catgaaggag cgcacgcgtg aactttttcc tctcttcccg
    48661 ctggtcggta cgcctggcgc tgatcattat cgccctgctg gcgctgattg cgctcaccag
    48721 ccagtggtgg cagccgtacg atccacaggc aattgatttg ccgtcgtgcc tgctttcgcc
    48781 ggatgcgcag cactggctgg gcaccgatca cttaggtcgc gatattttct cgcggctgat
    48841 ggcagcgacc cgcgtgtcgc tcggttcggt aatggcctgc ctgctgctgg tgctgacatt
    48901 agggctggtt attggcggca gcgccgggtt gattggcggg cacgttgatc aggccaccat
    48961 gcgcgtcgcc gatatgttta tgaccttccc gacctcgatt ctgtcgttct ttatggttgg
    49021 cgtgctcggc accgggctga ccaacgtaat tatcgccatc gccctgtcgc actgggcgtg
    49081 gtatgcacgc atggtgcgca gcttggtgat ttcactacgc caacgcgagt ttgtgctggc
    49141 gtcacggctt tccggtgcgg gccatgtgcg ggtgtttgtc gatcatctgg caggcgcggt
    49201 gatcccttcg ctgctggtgc tggcaacgct ggatatcggc catatgatgc tgcacgtcgc
    49261 ggggatgtct ttccttggcc tcggtgtgac cgcgccgacc gccgaatggg gcgtgatgat
    49321 taacgacgcg cgccagtata tctggaccca gccgctgcaa atgttctggc cggggctggc
    49381 gctgtttatc agcgtgatgg cctttaacct ggtgggtgac gcactgcgcg atcatctgga
    49441 tcctcatctg gtgacggagc acgcacacta atgccgcaac agattgaact acgtaatatc
    49501 acgctacagg ccgcgcagcc gctggtacac ggtgtatcgt taaccctgca acgcgggcgc
    49561 gtgctggcgt tagtcggcgg tagcggcagc gggaaatcat taacctgcgc cgcgacgctg
    49621 ggcattttgc ccgctggcgt tcgccagacg gcgggggaaa ttttagccga tggcaaaccg
    49681 gtttcgcctt acgccctgcg cggcatcaaa attgccacca tcatgcagaa cccgcgcagc
    49741 gcctttaatc cactgcacac catgcacacc cacgcgcggg aaacctgcct ggcgttaggg
    49801 aaacccgccg atgacgctac gcttaccgct gccatagaag cggtggggct ggaaaacgcc
    49861 gcgcgcgtgc tgaagctgta cccgttcgag atgagcggcg gcatgttgca gcgcatgatg
    49921 attgcgatgg cggtgctgtg tgaatcaccg tttatcatcg ccgatgaacc gaccaccgac
    49981 ctcgacgtgg tagcacaggc gcgcatcctc gatctgctgg aaagcattat gcaaaaacaa
    50041 gcgccgggaa tgctgctggt cacccatgat atgggcgttg tggcgcgtct ggcggatgac
    50101 gtggcggtga tgtcacaagg taaaattgtc gaacagggcg atgtagaaac gctgtttaac
    50161 gcccccaaac atacggtgac gcgcagcctg gtttccgctc atctcgccct ctacggtatg
    50221 gagctggcat catgacctta cttaacgtct gcggcctttc ccatcactat gcccacggtg
    50281 gatttagcgg aaaacatcaa catcaggcgg tgctgaataa cgtttccctg accctgaaaa
    50341 gcggcgaaac cgtcgccctg ctgggacgca gcggctgcgg aaaaagcacc ctcgcgcggc
    50401 tgctggtagg cttagagtct ccctcacaag ggaatattag ctggcacggc gaatcgctgg
    50461 cgaaacttaa tcgcgcccaa cgtaaagcct tccgccgtga tattcagatg gtgtttcagg
    50521 actccatcag tgccgtgaat ccgcgcaaaa ccgtgcgcga gatcctgcgt gaaccgatgc
    50581 gccacctgct atcactgaaa aaatccgaac aactggcgcg cgccagcgaa atgctgaagg
    50641 cggtcgatct cgatgacagc gttctcgaca aacgcccacc gcagttaagc ggcggccagc
    50701 tccagctcgt ctgcctggca cgcgcgctgg cggtcgaacc gaaattgctg attctggatg
    50761 aagccgtttc taaccttgat ctcgtgttac aggcgggcgt cattcgcctg ctgaaaaagc
    50821 tacaacaaca gtttggcact gcctgcctgt tcatcactca tgacttacgc ctggtggaac
    50881 gcttttgcca gcgagtgatg gtgatggaca acggacaaat cgccgaaacc caggcggtgg
    50941 gagataaatt aaccttttcc tctgacgccg gacgtgtgct acaaaacgcg gtattacccg
    51001 cattccccgt gcgccgtcgc acctcagaaa aggtttaacg ccaatgcaac gagtcaccat
    51061 cacgcttgat gacgatttac tggagacgct ggacagcctg agccagcgtc gtggttataa
    51121 caaccgttcc gaagctatcc gcgatattct gcgtagtgcc ctggcgcagg aagctaccca
    51181 gcagcacggc acacaaggtt tcgcggtgct gtcgtatgtg tatgaacacg aaaaacgcga
    51241 cttagccagc cgtattgttt ccacccagca tcatcaccac gacctctccg tcgccacgct
    51301 acatgtgcac atcaaccacg acgactgtct ggaaatcgcc gtgttgaaag gtgacatggg
    51361 tgacgtgcag cattttgccg atgacgttat cgcccagcgc ggcgtgcggc acgggcattt
    51421 gcagtgcctg ccgaaggaag attgagttat ttctaaaaca aggaaagaaa agatattaac
    51481 tgaacttaat ttataaaatt aagttcagtt gcatagatag atcctgtaaa ggcagatatt
    51541 ctgcgtaatg tacgtcgcta aaatttataa atgattcaaa cgctcattca gtgtttcaat
    51601 aatataggta ttaacagaga cttgttgctg cgcagccgca ttattaaggc gttcactcag
    51661 cgattctgga tagcgtaacg taaaggtctt aatcttttct gtacgcgcat acggctcaat
    51721 acccgcagct ttacaatcct caagatactc tcgcagggaa agctctcctt ctttttgcag
    51781 cccctgaata ctgtcagaga caaaatcaca ataacctgac aagcccagga atttgccacg
    51841 aaatgcattc agttctggca cgtaggtaat aacagctggt tgcccggcaa tctccataga
    51901 gtttggcgtc ttcagtttga tcatggtttt acccctatac ttagtaacca gtcacacacg
    51961 ctttctacag cgcctttatc ggtatctggc gacggatgag gccgatggaa acacgcaacg
    52021 ctcatattta gtatgaactt gcaacgcgaa cctcttcctt ccttaatttc tccgcctaat
    52081 gctttaacca gtgactctat atcagaccac ttgatccctt gaggaacagg agttttaaat
    52141 atttgatcca atgtattttt ttgtttcttc cgtaaagcct gtaccttggt tctcatggca
    52201 tgtccttatg acttcacttt atgacatcac agtatgaagt caagtttccc ttccttacgc
    52261 cattgtccca atcgtcttcc tgaatcgcag cagtgcgatg gtgaaaaacg cgccgccaat
    52321 tgccatcagc gtcaggaact gcggccagac gatttcgaat ccggcacccc ggtagaggat
    52381 ggcctgcgcg aggctaacaa agtgtgtcgt cggcatggtc agcataatgt cctgcaccat
    52441 ctgcggcata ctttcgcgcg gcgtggatcc accggaaagc atttgcagcg gcagcagcac
    52501 cagaatcacc agcagcccca gttgcggcat tgaacgcgct atcgtcccca taaaaatgcc
    52561 gattgacgtg gtggcaaaca gactgagcgc cacgcccagc ataaacagcg ggatcgagcc
    52621 ttcaatcggt acgcccagta cacctttcac catcagcacc agcgataatc ccgataccac
    52681 cagcaccacc agccccatcg accagatctt cgccatcatg atctcaaacg gcgttatcgg
    52741 catcaccagt aaatgttcca ccgtgccgtg ttcacgctcg cggatcagcg ccgatccggt
    52801 caatacaatc gccagcatgg taatgttgtt gatgatcgcc atcaccccgc caaaccacgc
    52861 gggatcaagg ttcgggttaa agcgcatccg ggtttccagc gataccaacg gttcgctgtt
    52921 atcacggtag cgcgcgacaa agctgttcac ttcaccgttg ataatattct ggatataccc
    52981 attgccggta aatgcctggc tcatgcgcgt ggcatcgacg ttcacctgaa tatccggctg
    53041 gcgtccggcg aggacatcac gctgaaaatt aggcggaata tttatcgcga aggtatagcg
    53101 tccggcgtcc agtccggcat ccatctcatc ggcggtgatc atctccggtg gcaaaaacca
    53161 cggacgatag aagctgttaa cgatccggtt cgataactgc gattgatcca tatcggcaat
    53221 ggcgatcggc gcgaggttca acgatcctgg cgtaacggtc gctgacgaat acaccgacac
    53281 cgtaaacgag aagacaatca gcgtcagcat cgctttatca ccgagcagac tgcgcaactc
    53341 tttgataccc agattaaaaa tattgcgtaa atggcgcatc atccctcctg ttttttcagc
    53401 agcaggatgc ttaagcccat caccagcggg atggctatca gcaacgggat aaaaagttgc
    53461 cacaaatcag tcagatccag cgctttcgag aacgttccgc gggcgatagt cagaaaatga
    53521 ctggtcgggt aaacctcgcc gatccaacgt ccaggccctt ccagcgaagc taccggatcg
    53581 atcatcccgg aaaactgtgt cgccgggatc aatgtgataa tcgccgttcc gaaaatggcg
    53641 gcaatctggc ttttcataaa ggtggagatc agcagcccca ttccagtggc aatgatgata
    53701 tacagcagcg ccgccaaggt gagcgtcagg aaactgcctt tatgcggtac gccaaacaca
    53761 aacaccgaca ggccgcagag caggaaaaag ttcagcatcc ccagcgcgat gtacggcagc
    53821 tgtttgccaa gcaaaaattc gctacgggtg gtgggggtca cgtaaaggtt gataatcgac
    53881 cctagctctt tttcccgcac cacgctaagg gcgcttagca ttgacgggat catcatcagc
    53941 agaagcggga tcaccgccgg aacaatcgct ggcaggcttt ttacgtccgg gttatagcga
    54001 taacgcgtct caatgttcat cagcccgctt tggctggcgg gtgtcgattg tcggctcgcc
    54061 acatcctgaa gccaactctg gtgcattgcc tgcacgtaac cttttaccgt ttcggcacga
    54121 ctgggcatcg ctccgtcgat ccagacgccg agttccacag gcgtaccacg cgcgatatcg
    54181 cgcccgaaat tgggcgggat ctcaatggcc accgtgatat cgcccgcacg catccgacga
    54241 tcaagctcgt cataactggt gagcggcggc tgttcgataa agtaacggga accggagagg
    54301 ttgagtgtcc acgcctggct actgacggtc tggtcgcggt cgagcaccgc aaagcgcagg
    54361 ttttccacat ccatactgat gccgtaaccc attatcagca tcaggatcac cgttcccatc
    54421 agcgccagcg tcgaacgtac tggatcgcgt cgcagttcca gcgcttcgcg gcggctgtag
    54481 ctaaacagac ggcgcaggct aaatccctga cgcggcgcgt gggtggtgtc gtgtaccacg
    54541 ggcggcgctt cggcttcgtt gctctgccct gccgcttcct gcaaataggc gataaatgcc
    54601 tcttccagac tggcggctcc gcgtttctca accagttcct gcggtgtacc gctggcaagc
    54661 acttttccgg cgtgcatcag tgagatgcgg tcgcaacgtt ccgcttcgtt cataaagtgg
    54721 gtggagatga agatagtcac tttgtcctgg cgcgagagat cgaccatcaa ctgccagaac
    54781 atatccctcg ccaccggatc gacaccagaa gtaggctcat cgaggattaa catctccggg
    54841 cgatgaatca ccgccaccgc cagcgaaagc cgctggcgaa tgccgagcgg caatgactcc
    54901 ggcagaacat cttcaacgtc gttgagctta aaacgctcgc tcatttcagc cactcgcgcg
    54961 ggaatttccg cttccgggat gtgaaacaaa cgggcatgta actccaggtt ttgccgcacg
    55021 gtgagttcgt tatagagcga aaacgcctgc gacatatagc ccacacgacg gcgggtatcg
    55081 atatcttttg gatcaaccgg ttgcccaaat agccacgcct caccttcgct ggcgggcagc
    55141 agtccggtaa gcattttcat ggtggtggat ttaccgcagc cgttcgaacc aaggaatcca
    55201 aaaatctccc cgcgtggaat gcggaaattg acgtgatcaa cggcaacgaa ggaaccaaaa
    55261 cgcatggtca gatcgcgcgc ttcgatggca atctctgcgt tttcaggttg atacggtggg
    55321 atcactaccg cctgatgcgc ctggcgttgc gcttgcggta acagattaat aaatgcttct
    55381 tccagcgtgg cgctttgcgt ttgctgccgc agctcttcgg cgctgccggt tgccagcact
    55441 tctccggcat tcatcgctac cagccagtcg aagcgttcgg cctcttccat ataggcggtg
    55501 gcgaccagca cgctcatatt gctctgccgc tggcgaatac tgtcgatcag atcccagaac
    55561 tgggcgcggg agagcgggtc aacccccgtt gttggctcat caaggatcaa cagttccggg
    55621 tcgtggatta acgcgcagca cagcccaagt ttttgcttca tcccgccgga gagtttacct
    55681 gccggacgat cgcgaaacgg tgctaacccg gtgctggtca gcagctcatt aattcgcgct
    55741 tcccgctccg ctttatcgtg accgaacagg cgagcgaaaa aatcgacatt ttcatacacc
    55801 gacaaggtgt ggtagaggtt tttgcccagc ccctgcggca tccaggcgat gcgcgggcag
    55861 acgtctcggc gatgcttcgg gtcgtgcata tcgccaccca gcaccaccac attgccctgc
    55921 tcaatgacgc gggcaccgga aatcagtgac aataaactcg acttcccgac gccatccgga
    55981 ccaatcagcc cgaccatgca acgggccgga atatcgagag tgatattgtt cagcgcaacg
    56041 gtttttccat aatgctggct cacgcccgcc agttgcgcga caggcgggac gggaaccagt
    56101 tccagatgcg tcattgcggc aacctcacca cgaggtcgtc aggccacgga agttcttcat
    56161 tcacccgcac ccacgctacg cccggtaaac cggttttgac atattccaga tgctgctgga
    56221 gtaattccgg tgggatacgc gctttgacgc ggaacatcag tttcagccgt tcatcgctgg
    56281 tttcgacggt ttttggcgtg aactgggcga cactggcgac aaaactgatg gttgcaggaa
    56341 tacgcagatc tggcgcggca tcgaggatca gccgggcttc accgcccagt ttcagcgtgc
    56401 ccgcctgttc ggttggcagg aagaaggtca tatagacgtc gctgagatcg accatattca
    56461 gcacccgacc gcctgccgcc agcacttccc ctggctcggc aacccgatac tgcacgcgtc
    56521 cgtcacgcgg ggctttcagt tcactgtcat cgatatctgc ggcaatgcgc cgttcagtgg
    56581 cttgcgctgc ttcgacccga gtttgcgcct gaatgatatt ggtgcgtgct gcttctatag
    56641 ccgctttaga agccgaaacc tgggctttcg ctgattccag cgcagctcgg gcgctctcag
    56701 cggcggcgcg gtcgtcatcc agctgttgcg cagaaatagc ccctcgttgg gcgagcgagc
    56761 gggaacgcgt atgacgtttg gcaacagagt ccagttcggc ctggcgttga ttgaccagcg
    56821 actgtgcggc acgagtttca ctttgtcgtt gctccagcaa agcctgcgcg gcggcaacgg
    56881 cgctttgtgc ctctttgatt tgcgcgatgg cttccagtcg ctgttcctgc aacacgcgag
    56941 tatccatctt cgccagcact tcgccttcgc gaacaaactg gccttctttc accagaatgg
    57001 tgtcgatacg cccggcaatt ttgctggcga tatccacttc cgtcgcttca atgcgcccat
    57061 tgctgacagc aaagccttcc ggcacacccg ccgggcgcaa cagccaccac gccacgacgg
    57121 cagccaccgc cagtgccccg acaacccacc acgccagatg gcgcttactc ttatccataa
    57181 tcgacccgcc ataatccctg taagcaaacg acgcgtgtta caactgccgc cgaacgacgt
    57241 tcagcggatt cttccatccg ggataagaag gaaacgacaa atacgaccag cgcgcagagc
    57301 attgagcgcc aacggggaag gcttagtgaa aacgggtagt aatgttgtca tcatcagcat
    57361 tcctggccgt aaatgaaaca cgcatactca taagtgtgat ctataaaact ggctgacaaa
    57421 acggtatcca tcctgttgga tcgcatttca cgcctgttca gttgcaggaa cagagcattc
    57481 ctttttacgt tttatcaaca gtctcaacca gatagtaaca ttaatattca tgttgtctat
    57541 ggttcagagc tgtaacattg tgcgccttct aatcaaaggc ataagtccat ttctgttttt
    57601 ttcatgctgg taagacttcc agggagtggt tatctctggc cctgcaccag ggttaatttg
    57661 gcaattatgc atgctgaaca ttcttccagc atgtttactg tcacgggcgt taagacctaa
    57721 aactgtcccc gtttcaccta tgcgttctct cccaccattt ccgttatgtg caacaacttg
    57781 atttatacct aacatttgtg aacatgctaa tagtgcttca agtttaggtc tgtaatctgt
    57841 cgaaacagaa aaataacgat taaaggatcg acaagagata gcccggctag ttttaaactt
    57901 tttgccatta attatagcat gatgcttttt attaataatt tccgctaatt cgaccggatc
    57961 gagagcattg gtcatttcat caatagagac agttatcggt cctataatgt aacgttttcg
    58021 ctgatcatca aaagttatcc catgatgatt tgccattatt ttgtatttgg ggtcatagaa
    58081 acatacagga taatctttta tcccgtcgta ggtatcctca tcaagtggtt tcatgcgcgt
    58141 gaagttttgc atatagttac cattgcaatt cgtttcatga tttccagcta atacatgaac
    58201 attttcattg atactcatta tgcgttttaa caaatcgatg ataaattgat cgccacagat
    58261 attactaaat cgatcgccgg tttggtcgcc aagaaacgta catggcgtga ctgcgtcttt
    58321 attaataacc aaataaggct gaagactttc taaaacatta tctcttctgt tcagtatact
    58381 gattattgta ttaacattag tgtcaagttg ctcattaaag ttctccctta gcaatacgtt
    58441 gagaacttca tcttctatat taagcaactg gctaaggaaa ttttctcctt cagccatgat
    58501 tcccaaatgt cctgttgcaa aaagcgcgta caaaactgca ccgactgagc catcggtatc
    58561 gccaaaatag gtcggcctgg tacttgtggg atcaacaggt gaacaattgg agaaataggt
    58621 tctgtcagcg aactggggtt ttataattgt ttcgaattta atatcaaata cattgtattg
    58681 cgatatttcc cagttccaga tgggtttgta cgactcagta actgcaacat cattagcatt
    58741 atcatttact gtcatattat taagtaaata aacaatgctg caccaattaa tatcggtatt
    58801 atcaagaggg caaccgtaat ttttactatt aattgtcaca ataaactgat aacagttatt
    58861 cctacgtaga atatgagcgc gcatgcccga tagcatacta tatttttctg aatgattcgt
    58921 ttgtatggta aatgaaattt tgtcatcttg aatagattgt tggaaacgtt ctatttgcca
    58981 atcatcaatt ttttccacaa cttcaccgag cattgtcctg gcaatacttg atatatcatc
    59041 acctatacca tttgcaatat acccaaacag ttctttagtg aagttggtaa catgctcttg
    59101 caccatatca tttgttgcga ttgtcgttgt actgccatta gtgcctgcca cagttccaat
    59161 tttcatcttt tacctcatat tattcaccgc gaggaataca tagttaacat tttatcttat
    59221 gacatacacc tcatattcga tggggaaata attatatttt gcacaaatca ttcagcctta
    59281 aagcataata tgaaatttct tatctcatag cagagaaagt ccctaaatat tgtagaggcg
    59341 tcatcagtac atacctctac aatcgattaa gaaaacctat gaaatactgg caaaattaaa
    59401 aagagatacc acagcactca tgaagtttta ttgaggttga aaacaactaa gccctgggac
    59461 acctttttta tcaccacgtt cgttaggtgc tatcttccaa gaatttagcc ccatggtata
    59521 attatcaatt ttttgatgct ggtctacccc taatccataa tggccaaatt tctgtttagt
    59581 ttttttgagc gtctgtccac atttgaagat atcttcaggt tttggtctga aagcagtagt
    59641 gttattaaaa taattttcat atacaggtgt gggtgctata gcattgcttc taattagatg
    59701 gagaagatga tcatccatgt gttgtttatg ttttttatta catatatttg ctaattcctc
    59761 aggatcaatt gggtctttca tctgattaaa tggaacctgc aaagcaccga gacaataaca
    59821 ttgattttct tcatttctta ttattccgtg atgactggtt aaaactttag tttctgagtt
    59881 ataattacaa acatcgagcg ttttaattaa gttatacgta tcaccggcag agagtttata
    59941 attttctagt cgtgcagtat agttaccatt aaaattgatt tcatgatttc cagccagaac
    60001 gatgacattt ttattaatac ggctatcttt attaccctcc atatttcgca tagaatttag
    60061 caaagtaagg atatatttat caccaaaaat agtactaaat ctatcgcctg tatgatcccc
    60121 aaggaagata caaggggtta tcgcatcgtc ctttaaaaaa acatgttgat gcaactcatt
    60181 caatatcatg tctctcgtat ttaataaatt gtagagcaat ttattatttt tatgtatgta
    60241 tgcagatgaa gccatgtctt cgtgttttaa taattcacat aataaactcc aacccttttc
    60301 cctgatgcct atatgccctg aaacgagcaa tgaataaagt acagcgccta ctcttccatc
    60361 tgtatcgcca aaataagccg tgttggcatt ttgtgggtta atcacaccac attgtttata
    60421 aaagtctatt ttagaaaaca cagatttaac atagctatca aacttatcct taggaaacgt
    60481 ctctttagag acatcccaac gccagtcagc tttaaaatta ccgctaagaa gagtaaagaa
    60541 attttcaggt atgatagaaa gcccattaac tttataaatt atgtctgatt tatctccgga
    60601 gaaattaata aaataataac catccttaca ctcaatctta actgtaatgg gagacgataa
    60661 ttttatcaca tggttattgt ctggtataat tgtaaagata aacgtgttag tagtattatc
    60721 ggcaaaacta ttatattctt gaatatctat agatggctca ctattgtaaa accatatttt
    60781 ctccatctga agactatgtt catttaccct ccagtgacca tgttgcagga aatagatgat
    60841 atgcatgcca aaatcgttat ctatttgcga atcattatcc agcttatcaa tttcaactat
    60901 ctttgaacga tgtgttttat caaccttgct atggtgttga tgattattaa ctttatctga
    60961 atgcttatct ttatggtttg aagggatgta cttaaatata ttgtcaattg ctttcataaa
    61021 taacccgcga tatttttgac ctgttagtaa aaaataaatg atacatgtct gaaagacata
    61081 tttacgacga tataaaacaa taatacttat atgttcgctg attttaagca ccgagactat
    61141 ccggtctttt actaaataat ggaaaacaat ccatccctgg aagacaattg taaaaaaaac
    61201 aacagaataa gaattaatct taggataaat atttatttat catggccttt tgaccgctaa
    61261 aaaatggcac tcggcacagt aaaccccgct atagttatgc tatgcatttg tctttaatga
    61321 tatctcgtcg ttatattaac aggctgaaat tatcattaat gcacatttca atattagcag
    61381 ggatacctga ggagtttaaa gtgaacatat atatcgggta gcttttcaaa ttaatccctt
    61441 tgattatagg cttaatttgc atcgcgttag gtggctttgt gctggaaagt tcagggcaac
    61501 gtcacgggtc atgtgctgat ttctctggcg gccatatgcc tggcattatt cactaccgca
    61561 tttattatca tttcgcaact cacgcgcggc gttaatacgt tttacaatac attgttcccc
    61621 attattggct atgcggggtc aattatcacc atgatatggg gttgggcact gttagcaggt
    61681 aatgatgtga tggcagacga gtttgttgcc ggccatgtta tttttggcgt tggtatgatt
    61741 gccgcatgtg tatcgacggt ggcagcgtcg tccggtcact ttctgctcat tcccaaaaat
    61801 gcagcgggga gcaagagcga cggaacaccg gtacaggctt attcttcatt aatcggtaat
    61861 tgcctcattg ccgttcccgt tttactcacc ctgctcggtt tcatttggtc tattacgctg
    61921 ttacatagtg ccgacataac tccgcattat gtcgccttgg gttaaccgca atctgtgcct
    61981 gtctaattgg cctagtggcc acaatcgtcc atcaaacacg taatacgttc tcatccaaag
    62041 agcactggct gtggtgttat tgggttattt ttctcggctc agtcacggtg ctgcagggga
    62101 tatacgtctt agtcagttcc gatgcaagcg cccgactggc tcccggcatt attctcattt
    62161 gcctcggaat gatctgttac agcatattct caaaagtctg gctactggca ctggtctgga
    62221 gacgtacctg ttcgttagcc aacagaatac cgatgatacc ggtatttacc tgtctgtttt
    62281 gcttgttttt gtcatcattc ctcgcggaaa tcgcacaaac tgagctggac tactttattc
    62341 catctcgggt tctggttgga ttaggcgcag tatgttttac attgttctct attgtttcaa
    62401 tattagaggc cggttctgcg aaaaaaaatt aaaatttaca taatcccagc gttattcata
    62461 tcttaacgct ggttttttac gaacttagtg aaataatgta gaatataaaa ttataatttt
    62521 atattggagc acatgtgtta atgatcaacc ccgggatact aaaaatatag acattattaa
    62581 acttgcttag cctcgcattg cctttacctg ttaaagatgc atcgagaaaa ttgagtctga
    62641 agttctcatc ctttatctga ttttccagtc ccgaaacttt ggccttagca ttacctgaga
    62701 tccgtccgtt actatctatt ttgtaggtaa aaaaatacga atgggaaaat ttcctcggtg
    62761 tatcttcatc agttaagcca acaatcgtaa aactaccgct accatctttt tttaaaatga
    62821 ctgaaaggtt agcagagtag tataaagaat gatctgtttt agcatttctt gttaacgtcg
    62881 acgtacattc aagagacgtt gtgctaccat cacgagcctt ccagagagca aaaaaacaca
    62941 cctgtagcaa tgtgaaaaca agtattaata taaaatacat tttcttatta gtcatataat
    63001 agcacctctg aacaaattgc ataagtttcg tcattagagg attttataca tcttgataaa
    63061 taccgctgcc cttccccaag caaataatgc atactattta catcatatat aaaatttgca
    63121 cctggcacac agggtaaatg ctttttcgaa atttcagcgg cctctatttg agatttagcg
    63181 atgccaatct cgttaagatt ctctgaaaga tataaatcac aggtgtcaat cttacctgat
    63241 ttaaccaatg gataatccgt tatactgtag gtatctttaa aagccacggc aaacatttca
    63301 ataataacaa ttactaccac aagcgaaatc aaaatgcgaa aaagaggaat actccttttg
    63361 cgctctactt tttcaacaac atcttcttgt aaatgagaat catcataaga ttgtttactt
    63421 attgttatgt tttcactgat gtaaaagcct actttgggta tcgtaagaat cacattttct
    63481 tcaacaccaa tatttttaaa acttttgcgt aacagggata tatattggtt caatgtgttg
    63541 tttgacagat caaatgaaaa tctctccaaa acgctatcaa tgattgagtt cctgtcaacc
    63601 gctccaccat tactttccag taacactgcc aaaatacacc ccgccgtagc tggaaaattt
    63661 atctcatcct gggtatttac attcgagagc gagcatttgt tcggatcaaa aataatcgcg
    63721 tcattgataa tgaaaaaaca tctccgctcc atgcaggcaa caacacaaaa taagattttg
    63781 tcattttagc ctattcctgg tggaactgac acaagaaact aaaatacaaa tataaccctg
    63841 aaaaagtcat ataaaactta tatccacaga ataaaaaatc aaacatgtca attttatcaa
    63901 tataaagtat cgcaaggcga aaagtgatgc aagaatactc ctaagtaaat gaaagcgcac
    63961 cacatgaaat tatcaacaca tgtaatttca tcattaggtg ctatcgaaaa taactcagga
    64021 gctacttaag atgaataatg tcttactcat gagacccgat gactcagaat ttatgatacc
    64081 tgatgactgg tcagggctaa acgcaaaagg cattacatac gaacaagata atagagtgac
    64141 agagtctttt ctgcctttta aatcttttta ttgttcaccg gtgtgcacga cagttgaact
    64201 catctcataa aataattgtt ttgctatggg tatccgctac cttctggaaa agaaaagaga
    64261 cttaaggcta aatcacttta aagccttctc tcataaatgc gagacaaaac ctgacgtgtt
    64321 aattatgacc gggtgcaccg tcgctgatac ttatagtata ttctcacgca ttgttgaaga
    64381 aaatataatg aaaacgatga cacctatcgt catcattact gggcatcact cagaagatct
    64441 ttgttctctg gcatcgaaac tgggaggagt taaaataata tcaccgacag aaaagattgc
    64501 aaaaatagaa aacgctatca atgatgcttt taacagcaag cctaaaaaaa taagattttt
    64561 aacagatata ctctctcctc aacaaattga gaccatgaaa atggttgcgc agggtagatc
    64621 cgttaatgag atttcagccg caatgaatat tagcatcaag accgtgtatg cacataaagt
    64681 tcgtgctatc aataagctgg gaattaagaa aaaagtttat gaagcgcttt tttataaagc
    64741 gctaattcag ttcaactgat gtaaaaatca ccctcgctta ataaataata tattctatgt
    64801 ctttttttat aagttaaaaa aggcgggata tcaaatcccg ccatgaaatt aactaatcat
    64861 ctgcattatt ctgcgtagat ataagcctgg gtacggatgg tgtaagagtc tggctcaaca
    64921 acctgttctt tcatatggga aacgacgcga taggcaccac ctgttccagt atttttcagc
    64981 atcatataac gcttaccgcc aatctcttcg gaggcagctg aaggaggatc ctgctcaagc
    65041 gttgtcaggg aaactttcag tttatggtcc gggttattgt caccagaaat tactgcatcc
    65101 gctgaagtac tgctctccgc ataaatatca tccgggaaag ttacagcata gtcgtggctc
    65161 tgcccatcag atgcgccaac aatacctgtt gccaaaggcg tccatgcagt taacgtaccg
    65221 ctatataccc ctttcactgg ttgaatggta ttattaacaa cgataggctt ggtcaaagta
    65281 agattagctg acgcttcagt accctggctt actaaagttc ccgcctgaga agcaaaagct
    65341 gtccccgcca gcatgattgc taacaatgtt ttcttcatta tattttctcc atgattttca
    65401 tttacactgc aaaatgcagt ttctttcttt gcatttacgt gctatcgcga aaatgcttcc
    65461 accaaataat ggaaacatct tcacaaaaat aaattatgag tttcttaatt tagaaactat
    65521 aaacatgtcg ccgctatctg gtataaccct gatgcacttt tcttcgcagg aagttggtat
    65581 tttgcgatac aggattgatc tttagaataa ccccatttca cattaaactc gccttccgca
    65641 ctcaagccag aaacataagc aataccatct tcacctacaa tgccggccat attttcagtg
    65701 tctttgctat aactaatcac agcgccgaag gggatgggtt taccctcttt ctgttttaag
    65761 gttactaatc ctttatcccc gctcttcact gccaatgtcg caggcacaac ggctccagct
    65821 gtcggtacaa ccaattgact agtttgtggt aagtcgtaat tcttacctat cgttttttca
    65881 tccagaataa catcgtctct tcggtatggc tgcactggca gtattgcata cccacgaaaa
    65941 tcagtactca gtcccggcca gccattaacg ttaacccccg ttgctccggg tgcctctata
    66001 agggctaatg tcccatccac attttgacca aatgtaagtc catcccttgt aataagtatc
    66061 ccaccgctta aatttgcacc tagctgtttc atgttttcgc tggagctgta atggctacta
    66121 aactgaccat atgatccatt tttgccaact tgcacaatgt tcatcgtgct cttcggttgt
    66181 gactgatgag cgttagttat atttgtcgct acatcccacc atacaccacc actattctga
    66241 cctgataatg aaacctgata gttattaccc tgctctcccg aacgactcat ctgtgaagac
    66301 gcataaattt gattttcgtc acccagcgat aatggcactg aaatacccac gcttaatttc
    66361 cgatctactt tccatttatg cgatgtcagc aagtcgtttt ttgcccagtt aatggagaat
    66421 gttatgcctc tccataccgt actataactc aggctggtag accgcgtgtt tcctgtgccc
    66481 cgggaagaat tatacctcgt ggtattgaag ttcaaattcc cgatattacc aaggggctgg
    66541 gataaggata tcgtagagcc atcgtgacta ttcttatctc tttttctgtt ccgttcatcc
    66601 tgtgcactta ctgcttcagt cagtgtggca taacctgatg cataacggtt cgtggttata
    66661 ga
//
LOCUS       DAJTUI010000010        66028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTUI010000010 DAJTUI010000000
VERSION     DAJTUI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.010"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="OO464_000704"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="OO464_000704"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6225963.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO464_000705"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO464_000705"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6225964.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO464_000706"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO464_000706"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6225965.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO464_000707"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO464_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6225966.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO464_000708"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO464_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6225967.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO464_000709"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO464_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6225968.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="OO464_000710"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="OO464_000710"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6225969.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10139
                     /gene="mrcB"
                     /locus_tag="OO464_000711"
     CDS             7605..10139
                     /gene="mrcB"
                     /locus_tag="OO464_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6225970.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10359..12602
                     /gene="fhuA"
                     /locus_tag="OO464_000712"
     CDS             10359..12602
                     /gene="fhuA"
                     /locus_tag="OO464_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6225971.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12653..13450
                     /gene="fhuC"
                     /locus_tag="OO464_000713"
     CDS             12653..13450
                     /gene="fhuC"
                     /locus_tag="OO464_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR6225972.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13450..14340
                     /gene="fhuD"
                     /locus_tag="OO464_000714"
     CDS             13450..14340
                     /gene="fhuD"
                     /locus_tag="OO464_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6225973.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14337..16319
                     /gene="fhuB"
                     /locus_tag="OO464_000715"
     CDS             14337..16319
                     /gene="fhuB"
                     /locus_tag="OO464_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR6225974.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="OO464_000716"
     CDS             complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="OO464_000716"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6225975.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17859..19280
                     /gene="clcA"
                     /locus_tag="OO464_000717"
     CDS             17859..19280
                     /gene="clcA"
                     /locus_tag="OO464_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6225976.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19304..19369
                     /locus_tag="OO464_000718"
     CDS             19304..19369
                     /locus_tag="OO464_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6225977.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19362..19706
                     /gene="erpA"
                     /locus_tag="OO464_000719"
     CDS             19362..19706
                     /gene="erpA"
                     /locus_tag="OO464_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6225978.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19753..20376)
                     /locus_tag="OO464_000720"
     CDS             complement(19753..20376)
                     /locus_tag="OO464_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6225979.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFSVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="OO464_000721"
     CDS             complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="OO464_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR6225980.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="OO464_000722"
     CDS             complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="OO464_000722"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6225981.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21989..23506
                     /gene="dgt"
                     /locus_tag="OO464_000723"
     CDS             21989..23506
                     /gene="dgt"
                     /locus_tag="OO464_000723"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6225982.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23636..25060
                     /gene="degP"
                     /locus_tag="OO464_000724"
     CDS             23636..25060
                     /gene="degP"
                     /locus_tag="OO464_000724"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6225983.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25215..26371
                     /gene="cdaR"
                     /locus_tag="OO464_000725"
                     /pseudo
     CDS             25215..26371
                     /gene="cdaR"
                     /locus_tag="OO464_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26460..26846)
                     /locus_tag="OO464_000726"
     CDS             complement(26460..26846)
                     /locus_tag="OO464_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6225984.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="OO464_000727"
     CDS             complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="OO464_000727"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6225985.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="OO464_000728"
     CDS             complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="OO464_000728"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6225986.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30749..31543)
                     /gene="map"
                     /locus_tag="OO464_000729"
     CDS             complement(30749..31543)
                     /gene="map"
                     /locus_tag="OO464_000729"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6225987.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31911..32636
                     /gene="rpsB"
                     /locus_tag="OO464_000730"
     CDS             31911..32636
                     /gene="rpsB"
                     /locus_tag="OO464_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6225988.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32894..33745
                     /gene="tsf"
                     /locus_tag="OO464_000731"
     CDS             32894..33745
                     /gene="tsf"
                     /locus_tag="OO464_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6225989.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33892..34617
                     /gene="pyrH"
                     /locus_tag="OO464_000732"
     CDS             33892..34617
                     /gene="pyrH"
                     /locus_tag="OO464_000732"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6225990.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34909..35466
                     /gene="frr"
                     /locus_tag="OO464_000733"
     CDS             34909..35466
                     /gene="frr"
                     /locus_tag="OO464_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6225991.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35558..36754
                     /gene="ispC"
                     /locus_tag="OO464_000734"
     CDS             35558..36754
                     /gene="ispC"
                     /locus_tag="OO464_000734"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6225992.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36943..37701
                     /gene="ispU"
                     /locus_tag="OO464_000735"
                     /gene_synonym="uppS"
     CDS             36943..37701
                     /gene="ispU"
                     /locus_tag="OO464_000735"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6225993.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37714..38571
                     /gene="cdsA"
                     /locus_tag="OO464_000736"
     CDS             37714..38571
                     /gene="cdsA"
                     /locus_tag="OO464_000736"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6225994.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38583..39935
                     /gene="rseP"
                     /locus_tag="OO464_000737"
     CDS             38583..39935
                     /gene="rseP"
                     /locus_tag="OO464_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6225995.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39965..42397
                     /gene="bamA"
                     /locus_tag="OO464_000738"
     CDS             39965..42397
                     /gene="bamA"
                     /locus_tag="OO464_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6225996.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42519..43004
                     /gene="skp"
                     /locus_tag="OO464_000739"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42519..43004
                     /gene="skp"
                     /locus_tag="OO464_000739"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6225997.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43008..44033
                     /gene="lpxD"
                     /locus_tag="OO464_000740"
     CDS             43008..44033
                     /gene="lpxD"
                     /locus_tag="OO464_000740"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6225998.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44138..44593
                     /gene="fabZ"
                     /locus_tag="OO464_000741"
     CDS             44138..44593
                     /gene="fabZ"
                     /locus_tag="OO464_000741"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6225999.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44597..45385
                     /gene="lpxA"
                     /locus_tag="OO464_000742"
     CDS             44597..45385
                     /gene="lpxA"
                     /locus_tag="OO464_000742"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6226000.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45385..46533
                     /gene="lpxB"
                     /locus_tag="OO464_000743"
     CDS             45385..46533
                     /gene="lpxB"
                     /locus_tag="OO464_000743"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6226001.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46530..47126
                     /gene="rnhB"
                     /locus_tag="OO464_000744"
     CDS             46530..47126
                     /gene="rnhB"
                     /locus_tag="OO464_000744"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6226002.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47163..50645
                     /gene="dnaE"
                     /locus_tag="OO464_000745"
     CDS             47163..50645
                     /gene="dnaE"
                     /locus_tag="OO464_000745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6226003.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50658..51617
                     /gene="accA"
                     /locus_tag="OO464_000746"
     CDS             50658..51617
                     /gene="accA"
                     /locus_tag="OO464_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6226004.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51716..53857
                     /gene="ldcC"
                     /locus_tag="OO464_000747"
     CDS             51716..53857
                     /gene="ldcC"
                     /locus_tag="OO464_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6226005.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53914..54303
                     /locus_tag="OO464_000748"
     CDS             53914..54303
                     /locus_tag="OO464_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6226006.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54368..55666
                     /gene="tilS"
                     /locus_tag="OO464_000749"
     CDS             54368..55666
                     /gene="tilS"
                     /locus_tag="OO464_000749"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6226007.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55715..55975)
                     /gene="rof"
                     /locus_tag="OO464_000750"
     CDS             complement(55715..55975)
                     /gene="rof"
                     /locus_tag="OO464_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6226008.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55962..56162)
                     /locus_tag="OO464_000751"
     CDS             complement(55962..56162)
                     /locus_tag="OO464_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6226009.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56328..56873
                     /locus_tag="OO464_000752"
     CDS             56328..56873
                     /locus_tag="OO464_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6226010.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56870..57292
                     /gene="arfB"
                     /locus_tag="OO464_000753"
     CDS             56870..57292
                     /gene="arfB"
                     /locus_tag="OO464_000753"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6226011.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57306..58016
                     /gene="nlpE"
                     /locus_tag="OO464_000754"
                     /gene_synonym="cutF"
     CDS             57306..58016
                     /gene="nlpE"
                     /locus_tag="OO464_000754"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6226012.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58171..58995)
                     /locus_tag="OO464_000755"
     CDS             complement(58171..58995)
                     /locus_tag="OO464_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR6226013.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59048..60766)
                     /gene="proS"
                     /locus_tag="OO464_000756"
     CDS             complement(59048..60766)
                     /gene="proS"
                     /locus_tag="OO464_000756"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6226014.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="OO464_000757"
     CDS             complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="OO464_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6226015.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="OO464_000758"
     CDS             complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="OO464_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6226016.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="OO464_000759"
     CDS             complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="OO464_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6226017.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62958..63611)
                     /gene="metI"
                     /locus_tag="OO464_000760"
     CDS             complement(62958..63611)
                     /gene="metI"
                     /locus_tag="OO464_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6226018.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63604..64635)
                     /gene="metN"
                     /locus_tag="OO464_000761"
     CDS             complement(63604..64635)
                     /gene="metN"
                     /locus_tag="OO464_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6226019.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64823..65398
                     /gene="gmhB"
                     /locus_tag="OO464_000762"
     CDS             64823..65398
                     /gene="gmhB"
                     /locus_tag="OO464_000762"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6226020.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65762..>66028
                     /locus_tag="OO464_000763"
     rRNA            65762..>66028
                     /locus_tag="OO464_000763"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15625 a        16343 c        18197 g        15863 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag
     1681 acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc
     2341 tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc
     2521 agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg
     5701 tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc
     5881 gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc cgcggcgcaa agtgagccgg agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc
     7261 cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa
     7321 aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cccgcggcag
     7921 tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac gaaatggtga
     7981 agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
     8101 aagggcaggt gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc accatgatct
     8221 cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga atcagtctct
     8341 actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt tcttactggc
     8461 gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8521 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8581 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     8641 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     8761 ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag ccgcgtggtg
     8821 gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg caggcaaaac
     8881 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     9301 tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     9361 tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag ttgcatccgg
     9421 ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc
     9481 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     9541 aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9721 tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg gtaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
    10021 atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag
    10081 agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac
    10141 atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag
    10201 atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg
    10261 taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc
    10321 gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc
    10381 agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt
    10441 ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac
    10501 ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg cagtcagcta
    10561 ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg
    10621 ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg
    10681 gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt
    10741 ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg cagggcaact
    10801 tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt atgcgtggcc
    10861 cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc
    10921 gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac agcctgttcc
    10981 agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct tatcgcctga
    11041 ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta
    11101 ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc ctttcttact
    11161 tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc
    11221 cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct
    11281 attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta
    11341 ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt
    11401 acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag
    11461 cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact
    11521 tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc
    11581 tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg
    11641 actctgtgcc actgttcaat ctgtacaatc cggtgaatcc cgatttcgac ttcaatgcca
    11701 aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa acgggcgttt
    11761 atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt cgttatgact
    11821 gggcagatca agaatctctt aaccgcgttg ctgggacgac cgataaacgt gatgacaaac
    11881 agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca
    11941 gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac
    12001 cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg
    12061 tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg gaccctgagg
    12121 gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa atcgaagcga
    12181 aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat
    12241 acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt
    12301 cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg
    12361 gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa gtgggaagtt
    12421 atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg gcgggctcca
    12481 acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca
    12541 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc tttcgtttct
    12601 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
    12661 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
    12721 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
    12781 tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc
    12841 gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt
    12901 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
    12961 tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc
    13021 agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg
    13081 gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc
    13141 gcaggatagc cgttgtctgt tgctcgacga accgacttca gcgctggata tcgcccacca
    13201 ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc
    13261 cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcag
    13321 tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat
    13381 gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt
    13441 tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg
    13501 ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga ccccaatcgt
    13561 attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac
    13621 ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca
    13681 gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg
    13741 tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc acgtattgcg
    13801 ccgggtcgcg gatttaactt cagtgacggc aaacatccgt tggcgatggc gcgtaaatcg
    13861 ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca tttagcgcaa
    13921 tacgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta
    13981 ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc
    14041 caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg
    14101 ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt
    14161 tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc
    14221 atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt ttatggtgca
    14281 acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg taaagcgtga
    14341 gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt gtcgctacgg
    14401 cgctcacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg caggccgcct
    14461 ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc
    14521 tggcgatttc gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag
    14581 tactgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg
    14641 ggattaccgt caccacgctc tgggcgatcc ctagcgcgat ggcgagccag tttgctgcgc
    14701 tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggctgt
    14761 cgccggtaac gctgattttc gcggggttgg tagtgagcct ttattgcggc gcaatcaatc
    14821 agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg agcactggaa
    14881 cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg ctgggcggtg
    14941 tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg
    15001 cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg ctggcgattg
    15061 tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg ttgttcgcgc
    15121 cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg ctggcgtcgt
    15181 tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt
    15241 ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc
    15301 tgctgccgcg tttacgcagc attagcgcgc cggatatgaa ggttaacgat cgtgtcgcga
    15361 ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg ttgatggctg
    15421 tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt
    15481 tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg
    15541 tcatgctggc ggtagcgggc tgtattattc agcgactgac cggcaacccg atggcaagcc
    15601 cggaagtgct ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg ctgtttctgg
    15661 taccgggtaa tgcctttggc tggttgttac ccgcaggcag tctcggggcg gcggtgacgc
    15721 tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc atgttactgg
    15781 cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg
    15841 acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg
    15901 ctcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattact ccgctgtgcc
    15961 gccgctggct gaccatttta ccgctgggtg gtgataccgc gcgggcggta gggatggcgc
    16021 tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga
    16081 tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg
    16141 gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg
    16201 tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc
    16261 tgtcgacctt tatcggtgcg ccatatttta tctatttgct gagaaagcag agccgttaat
    16321 agaatgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca cccgacgtgc
    16381 agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa agcctgcttc
    16441 aaacgctgac ggtgccaggt aaacaccttc atccagcatc atatggaaga aacgcttaaa
    16501 acgctcgacg tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa
    16561 gaagataccg aacataccac cgacgtggtt aacgaccagc ggaattcccg cttcttctgc
    16621 cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaatgcc
    16681 cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg ggttaccgga
    16741 aagcgtaccc gcctgataga ccggacctgt cggggccagc gcatccatta catcacgacg
    16801 accaccgaat gcgcctaccg gcattccacc gccgatgatt tttccgaggc aggttaaatc
    16861 tggcactacg gcgaaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac
    16921 ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac ctggcaggaa
    16981 ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc
    17041 aatctcttgc gggtattgct caaacgcggc gcgtacagag gccagatcgt tataagtaca
    17101 ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct ggcctaacgt
    17161 gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc
    17221 aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatcgt
    17281 cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac
    17341 cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc
    17401 ggcttcaatc acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca
    17461 ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc
    17521 tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac
    17581 accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg
    17641 ttcctgattc gtagaaaaag tgaatggctg ctattccatg ttattcataa caagttaaat
    17701 actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt
    17761 tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg
    17821 ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt
    17881 tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc
    17941 gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc
    18001 tggctgcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc
    18061 tggtacatac tgctgataat tatccgctac tgttaaccgt cgcttttctc tgttcggcgg
    18121 tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca gggggatcgg
    18181 ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg tggcgtgtat
    18241 tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatgggg ctagggcgcg
    18301 aaggaccaac cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat attttccgcc
    18361 tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg gggctggctg
    18421 cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt
    18481 ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta
    18541 tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc
    18601 cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta
    18661 tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata
    18721 ttaccaaatg ggtactgatg gggggggcga ttggcggtct gtgtggattg ctggggtttg
    18781 tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcggggaatt
    18841 tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc ttactctgct
    18901 tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg
    18961 gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg gaggcgggga
    19021 cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg ccgttaacgg
    19081 ggatcattct ggttctggag atgaccgata actaccagct cattttgcca atgattatta
    19141 ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaacctcta tactcggcga
    19201 ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat
    19261 cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg
    19321 gttagaattt gcccgattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac
    19381 tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag
    19441 ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt
    19501 atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg
    19561 ttggcctggt ggttgatccg atgagcctgc aatatctggt cggcggttcc gttgactaca
    19621 ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg
    19681 gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa ctatccggca
    19741 catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca
    19801 acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc
    19861 cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc
    19921 agttgcgtag atttctgtac gtaaaatcat ggggatttcg cggaccagta catcacgaat
    19981 gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc
    20041 cgcattaaag gctttattca cgctaatgcc gacaaacacc gccagaccaa cggcgtccag
    20101 caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat
    20161 gctggtgacc atggcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg
    20221 atccagcgcc atgtcgcgaa ttgttccgtc gcctactgcg gtaaccacgc ccagtaccag
    20281 aacactaaaa gggtccatac gcaattttcc ggctaacaaa acgccgtaga tggcaaatac
    20341 cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac tgagcacctg
    20401 aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt gctgcgagga
    20461 taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg ggaattttga
    20521 gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacaa
    20581 tagcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag ggaacccggc
    20641 tgtctttaaa gatgttttct ccgccacaaa cttcgagtac ctggttctga atcgactctt
    20701 ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat
    20761 cagcatattg cgctttcaat tgcacgtact gatccagcag ggattgcgcg gcttgttcgg
    20821 ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa
    20881 tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg tcaacctgcc
    20941 gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt
    21001 ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt
    21061 cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg
    21121 cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgcg agaaaagaca
    21181 gggcgaccag cgccctgaac agtgatttag ccatgtgcca gtttctgcac cagtgactca
    21241 accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac
    21301 tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt gaaattgtgg
    21361 cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg
    21421 cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac aatcaggcca
    21481 cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg
    21541 tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg
    21601 acatccgcgt cgtgataacg cgcttcgtcc gagacaacga tatcgcccac tttcagcgtt
    21661 ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca gtgttccaac
    21721 agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc
    21781 tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacgg
    21841 ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt
    21901 ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg
    21961 gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa
    22021 ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat
    22081 cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa
    22141 gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat
    22201 ggaagtccag caggtgggac gctacatcgc caaagaaatt ttaagccgtc tgaaagagct
    22261 taaattgctg gaagcatacg gcctggatga actgaccggc ccctttgaaa gcattgttga
    22321 gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcagc
    22381 gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc agcctctgac
    22441 tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac cgcttaacga
    22501 gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac aaggcattcg
    22561 tctggtgcat acattgatgc ggatgaatct cacttgggca caggttggcg gtattttaaa
    22621 atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact atttaatgaa
    22681 aaagccgggt tattatcttt ctgaagaagc ctatattgcc cgcttgcgta aagaacttaa
    22741 tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg acgacatctc
    22801 ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct
    22861 ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt
    22921 ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca
    22981 gttttttatg tatttacggg taaacaccct aaataaactg gtaccctacg cggcacaacg
    23041 atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat tattggaaga
    23101 tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa aacatgtgtt
    23161 tagccatccg gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt
    23221 agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac tggtagaaaa
    23281 agaacgggtg aaacgtttcc ctattgagtc gcgcttattc cacaaactct cgacgcgcca
    23341 tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct
    23401 atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct
    23461 ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag tcttttgtaa
    23521 agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc gggctataaa
    23581 acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga aatacatgaa
    23641 aaaaaacaca ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct
    23701 ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc
    23761 accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac
    23821 cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt
    23881 ctgccaggaa ggttctccgt tccagagctc tccgttctgc caggggggcc agggcggtaa
    23941 tggcggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca ttgatgccga
    24001 taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtga ttaaagtcca
    24061 gctgagcgat ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc gctctgatat
    24121 cgcgctgatc caaatccaga acccgaaaaa actgaccgca attaagatgg cggattctga
    24181 tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggcgagac
    24241 ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg aaaactacga
    24301 aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa
    24361 cctgaacggc gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat
    24421 cggtatcggt tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc agatggtgga
    24481 atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga attccgaact
    24541 ggcgaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa
    24601 ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa
    24661 gccgatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg tgggcagcaa
    24721 actgaccctg ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg aactgcagca
    24781 gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat
    24841 gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaagacgg gcactccggc
    24901 tgcgcagatc ggcctgaaga aaggtgatgt gattattggt gcgaaccagc aggcagtgaa
    24961 aaacatcgct gaactgcgta aagttctcga cagcaaaccg tctgtgctgg cactgaacat
    25021 tcagcgcggc gacagcacca tctacctgtt aatgcagtaa tctccctcaa ccccttcctg
    25081 aatacgggaa ggggttctcc ttacaatctg tgaacttcgc cacaactcca tacatcttca
    25141 tcatcctttg ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc
    25201 ataacggagg acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg
    25261 cacgtaccat gcgcatcatc gataccaata tcaacgtaat ggatgcccgc gggcgaatta
    25321 tcggcagcgg cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt
    25381 cacagggacg agtcgtcgat atcgatgacg cggtagcacg tcatctgcac ggtgtgcggc
    25441 aggggattaa tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgacag
    25501 gtgaaccaga gaatctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc
    25561 tggaacagtc gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg gaagaactgg
    25621 tgatgaacct gattcaggca gaggagaata ctcccgcact tctgaatggg cgcaacggct
    25681 ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct
    25741 tggcgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga
    25801 gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt
    25861 gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac
    25921 ccgcatgaaa gagtacggcc agctgcgttt tcgcgtttca ctgggcaact attttaccgg
    25981 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca
    26041 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    26101 cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc
    26161 gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgcc acaatgtgca
    26221 accgctggca acgtcaaagg cgttgtttat tcatcgtaat accctggagt atcggcttaa
    26281 tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta
    26341 tgtggcgtta caactggatg aagagcggta gattatgcgt taaggttgag gcggcgctgg
    26401 ctcattcccc tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc aattcgggat
    26461 tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact
    26521 gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca
    26581 cgctgacaga tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg
    26641 acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat
    26701 ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga
    26761 atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata
    26821 cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct
    26881 tattttagca tttggcttgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt
    26941 ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc
    27001 atgggatgga ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga
    27061 tgaaaaagta tttaaaaaag ggggaggaag aatgagtagg acgaattatt ctgagccaga
    27121 aagcatacgg gcagcatcat gctgcccgta tatgtactct taatcgatgg tacgcagcag
    27181 ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca
    27241 gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc
    27301 cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc
    27361 aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt caaccacttc
    27421 agagcgtgcg ccaatgaagc agttatcttc aataatggtc gggttagcct gcagcggctc
    27481 cagcacgccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca
    27541 agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt
    27601 aacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc
    27661 tggtggcaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt
    27721 catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat
    27781 acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat
    27841 tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat ttaccgcttc
    27901 gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa aagcggtttc
    27961 aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc
    28021 tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt
    28081 attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat
    28141 ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactcg
    28201 cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc ggtcggtatg
    28261 ggtcggcaaa aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg
    28321 acggggctgc ggtggctgcc agctactttg cgtcagcacc tgctccagac caaaccgaat
    28381 aacctcatgg cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc
    28441 catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg
    28501 gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat
    28561 ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc agcggtttgc ttaaatcatg
    28621 ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt
    28681 agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag
    28741 tagtgccagt gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg
    28801 cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt
    28861 ccagctattc cacagtgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag
    28921 atagcgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc
    28981 ctgaatatcg cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc
    29041 aaccagctgt gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg
    29101 agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc
    29161 gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg
    29221 ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc
    29281 atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg
    29341 cggcatatac gcgccgagca cgctatggcg atgcattggc aatagtccgc gccgcaccgc
    29401 tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tgcggaatat tacacagcgg
    29461 ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt
    29521 gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat
    29581 aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgag
    29641 tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca gttgcagcag
    29701 catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc
    29761 gaccggttcg ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc
    29821 gaataacaga cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca
    29881 cagtatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc
    29941 gaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg
    30001 aatatcgcgc aagccgccag ggctactttt gatgtctggt tcaaggttgt agctggtgcc
    30061 atggtaacgc tgatggcgct ggttctgttc ttcaactttc gctgcgtaga acttgtcgga
    30121 aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc
    30181 aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccaa
    30241 catgcactct tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag
    30301 cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat
    30361 cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcaac
    30421 caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg
    30481 gtctatgaac tcggtacgtg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc
    30541 atcacccagc caacgctgga aagtatcgat atgggctttt atcccacaga cggttaattc
    30601 atcacggggc caggcgcatg gattttgcgg ttgaccgggc agggtggaga gagcggtgtt
    30661 tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa ttatcgcatt
    30721 aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg
    30781 tgtcatcctt gcgtagcgtc aaaatttcgc agccgttatc agtcaccaca atagtatgct
    30841 catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc
    30901 ggatctcttt tttacccgcg ttgaccattg gctcaatggt gaacgtcatc cccggtttca
    30961 gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc
    31021 gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaatt
    31081 tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg cgtagcgcca
    31141 ggtacaggct ttcttgcgtg atgcggcaca ggcgttcgcc catgatggtc ggcttaccga
    31201 cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt
    31261 taacgatatc gccatctttc agcagcttag catcgtccgg gataccgtgg cacaccactt
    31321 cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg
    31381 cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc
    31441 ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc
    31501 gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc
    31561 gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt
    31621 gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat agaaaattcg
    31681 acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat ttgtggtata
    31741 aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca
    31801 ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa
    31861 tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg
    31921 tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga
    31981 acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg
    32041 agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt gtttctcgca
    32101 aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg
    32161 ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact
    32221 ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg
    32281 gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac
    32341 tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa
    32401 tcgatgctga ccacgaacac attgctatca aagaagcaaa caacttgggt attccggtat
    32461 ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg
    32521 acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag
    32581 gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag
    32641 gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc
    32701 gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg
    32761 aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc
    32821 atatggcccc ctttttcact tttatatctg tgcggtttaa tgccgggcag atcacatctc
    32881 cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac
    32941 tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct
    33001 ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt
    33061 tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt
    33121 taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt
    33181 tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga
    33241 agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc
    33301 tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt
    33361 tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag
    33421 caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca
    33481 ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt
    33541 tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat
    33601 ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt
    33661 catccgtttc gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt
    33721 tgctgcgatg tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt
    33781 ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca
    33841 atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc
    33901 aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc
    33961 actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa
    34021 ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc
    34081 gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg
    34141 accgtaatga acggcttggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt
    34201 ctgatgtccg ctattccatt gaatggcgtg tgcgacagct acagctgggc agaagctatc
    34261 agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt
    34321 accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa
    34381 gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagatccaac cgcaaccatg
    34441 tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg
    34501 gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg
    34561 ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc
    34621 ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt
    34681 ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac
    34741 aaaagtacgt gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa
    34801 taatccagac gattacccgt aatatgttta atcagggcta tacttagcac acttccactg
    34861 tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat
    34921 atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc
    34981 agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtggaatat
    35041 tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact
    35101 ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcc
    35161 tccgatctcg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg
    35221 ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg
    35281 cgtgttgcag tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa
    35341 gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact
    35401 gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag
    35461 ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc
    35521 gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg
    35581 ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt
    35641 ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga
    35701 attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat
    35761 gctacagcaa caggggagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat
    35821 ggcagcgctt gaggaggttg atcaggtgat ggcagccatt gttggtgctg ctgggctgtt
    35881 acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact
    35941 ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc
    36001 ggtcgatagc gaacataacg ctatttttca gagtttaccg caacctatcc agcataatct
    36061 gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg
    36121 ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc aagcttgccg
    36181 tcatccgaac tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa
    36241 aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc agatggaagt
    36301 gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct
    36361 ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa
    36421 tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc
    36481 cgcaccggat tatgagcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg
    36541 ccaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc
    36601 gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg aaaaaatgga
    36661 tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgagtgaagt
    36721 cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtcgcg
    36781 ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg
    36841 ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag gcttcagtat
    36901 tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa
    36961 ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat tatggacggc
    37021 aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa agccggggca
    37081 aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc gttaacgctg
    37141 tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa
    37201 ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa cgtgcgtctg
    37261 cgtattattg gcgataccag tcgttttaac tcgcgtttgc aagaacgtat tcgtaaatct
    37321 gaagcgctaa cagccggaaa taccggtctg acgctgaata ttgcggcgaa ctacggtgga
    37381 cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg
    37441 caaccagatc agatagatga agagatgtta aaccagcatg tctgtatgca tgaactggcc
    37501 cctgtagatt tagtaattag gactggggga gagcatcgca ttagtaactt tttgctttgg
    37561 caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt cgatgaacaa
    37621 gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag
    37681 cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct gatatctgct
    37741 tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc
    37801 attgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct tagcggtttt
    37861 accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt ggcgctgatg
    37921 ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga aatctcactt
    37981 tgggcttcgt tgggttggtg gattgtcgcg ctattgctgg tgctgtttta cccaggttcc
    38041 gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct aaccattgtt
    38101 cccttcttct ggggcatgct ggcgttacgg gcctggcact atgacgagaa tcattacagt
    38161 ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc cggcgcatat
    38221 atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc gggtaaaacc
    38281 tggcaaggct ttataggtgg actcgctact gcagcggtaa tctcatgggg ttatggcatg
    38341 tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt cgcagcgtta
    38401 gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg aattaaggac
    38461 agcggtcatt taattccagg acacggtggt attttagatc gtattgatag cctgacggct
    38521 gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta acggaaggta
    38581 atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt gtacttatca
    38641 ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt
    38701 tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc accgaatatg
    38761 ttatcgccct gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg
    38821 tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa cgagcggcga
    38881 ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac tggctggttt
    38941 ttattattgg tgtgcctggc gtacgtccgg tggttggcga aatagcagcc aattcgatag
    39001 ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc
    39061 ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa agcaccacca
    39121 ttacagtagc gccatttgac agcgaccaac ggcgggatgt aaagctcgat ttacgtcact
    39181 gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt cctcgtgggc
    39241 cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt
    39301 tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag tgggtgacct
    39361 ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc gaaaggcagg
    39421 ggagtccctt gtctttgaca ttaatcccgg agagtaaacc gggtaatggt aaagcgattg
    39481 gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa gttgtacgcc
    39541 agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc
    39601 tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac aacctcagtg
    39661 ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta gtttattacc
    39721 tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt ccgttgcccg
    39781 tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat
    39841 ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg ctgttaatgg
    39901 ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag aacgcataat
    39961 aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg ccaccgtata
    40021 cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt
    40081 tggtgcggcc ctcctcagta tgccggtgcg tacaggcgac acggttaatg atgaagatat
    40141 cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc gcgtccttcg
    40201 tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca gcattacttt
    40261 ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg cttctggtgt
    40321 gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag gtctggaaga
    40381 cttctactac agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga ccccgctgcc
    40441 gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg aaatccagca
    40501 aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc atttccaact
    40561 gcgtgacgaa gtgccgtggt ggaatgtggt aggcgatcgt aaataccaga aacagaaact
    40621 ggcgggcgat cttgaaaccc tgcgcagcta ctatctggac cgcggttatg cccgtttcaa
    40681 catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa
    40741 catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca accttgccgg
    40801 gcactccgct gaaattgagc aactgactaa gatcgagccg ggcgagctgt ataacggcac
    40861 taaagtgacc aaaatggaag atgacattaa aaagcttctc ggtcgctatg gttatgccta
    40921 tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt
    40981 gaacgttgat gcgggtaacc gtttctacgt gcgtaagatc cgttttgaag gtaacgatac
    41041 ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat ggttggggag
    41101 cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg aaactgtcga
    41161 taccgatacc caacgtgttc cgggtagccc ggaccaggtt gatgtagtct acaaggtgaa
    41221 agaacgtaac actggtagct tcaacttcgg gattggttac ggtactgaaa gtggcgtgag
    41281 cttccaggct ggcgtgcaac aggataactg gttaggtaca ggttatgctg ttggtatcaa
    41341 cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc cgtacttcac
    41401 cgtagatggc gtaagcctcg gtggtcgtct cttctataat gacttccagg cagatgacgc
    41461 cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg gcttcccgat
    41521 taacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc tgtccaacat
    41581 gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc cgagcacctc
    41641 tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt ggacctataa
    41701 caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga ccggtaaagt
    41761 gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg cgacttatgt
    41821 gccgatcgat gacgatcaca aatgggttgt tctgggtcgt acccgctggg gttatggtga
    41881 tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg gttccagcac
    41941 cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc cgcatcaggc
    42001 cagtaattat gatccggact atgattacga atgtgcgact caggacggcg cgaaagacct
    42061 gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc tcgagttcat
    42121 caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt cattcttctg
    42181 ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg gatatccgga
    42241 ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc
    42301 attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg gagacaaggc
    42361 agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag
    42421 tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac gcaaagaact
    42481 gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg ttattagctg
    42541 caggtcttgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca
    42601 atatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac acgctggaaa
    42661 atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg caggctaaaa
    42721 tgaaaaaact gcagtccatg aaagcgggca gcgatcgcac caaactggaa aaagacgtga
    42781 tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc
    42841 gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg aaatccgttg
    42901 ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac aacagcagcg
    42961 atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg ccttcaattc
    43021 gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc gatatcgtca
    43081 tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc atggttaacc
    43141 caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg
    43201 atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg acttacgcgc
    43261 gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca cccagtgcgg
    43321 tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac gcggtgattg
    43381 agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa
    43441 acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac catgagatcc
    43501 agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac ggctttggtt
    43561 atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta attattggcg
    43621 atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat gacactgtta
    43681 ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc gtgattggcg
    43741 acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact
    43801 gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg
    43861 ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat tcctcaggca
    43921 ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg aacattgatg
    43981 acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac taacgttcca
    44041 tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca ggccgtgtta
    44101 ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca ctcatactct
    44161 gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac tggtggatcg
    44221 cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga
    44281 gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc tgattctgga
    44341 agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac tggaaccggg
    44401 tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg
    44461 cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga cccgttttaa
    44521 aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag
    44581 ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg ccattgtgga
    44641 agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg ttggacccca
    44701 tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa
    44761 aattggccgc gataatgaga tttatcagtt tgcctccatc ggcgaagtta accaggattt
    44821 gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag
    44881 cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg gcagcgacaa
    44941 cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc gctgtattct
    45001 cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga tcatcggcgg
    45061 catgaccgca gtccatcagt tctgcatcat tggtgcgcat gtgatggttg gcggctgctc
    45121 cggtgtggcg caggacgtcc ctccttatgt tattgcgcag ggtaaccacg caacgccgtt
    45181 cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga ttaccgctat
    45241 ccgcaatgcg tataagctaa tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga
    45301 aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt tctttgcacg
    45361 ctcaacgcgc ggtctgattc gttaatgact gaccagcgtc cattaacgat tgccctggtc
    45421 gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct gaaagaacgt
    45481 gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa
    45541 gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct cggtcgtctg
    45601 cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact gaagccagat
    45661 gtttttgttg gtattgatgc gcccgacttc aatattactc ttgaaggtaa cctcaaaaag
    45721 cagggtatca aaaccattca ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc
    45781 gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg
    45841 ttttatgaca aatacaatgt accgtgccgc tttatcggtc ataccatggc tgatgccatg
    45901 ccgttagatc cagataaaaa tggtgcccgt gatgtgctgg ggatccctta cgatgcccac
    45961 tgtctggcat tgttgccggg cagccgtggt gcagaagtcg aaatgcttag tgccgatttc
    46021 ttgaaaactg cccagctttt gcgccagaca tatccggatc tcgaaatcgt ggtgccgctg
    46081 gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctg
    46141 tcggttcatt tgctggatgg aatgggccgt gaggcgatgg tcgccagcga tgcggcacta
    46201 ctggcgtcgg ggacggcagc cctggagtgt atgctggcga aatgcccgat ggtggtggga
    46261 tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac tgattatgtc
    46321 tcgctgccaa atctgctggc gggcagagag ttagtcaaag agttattgca ggaagagtgt
    46381 gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac
    46441 gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc cgatgagcag
    46501 gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg cacacgcagc
    46561 tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg
    46621 cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa aagctgagcg
    46681 aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc tggagtctgg
    46741 gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc atgctggcga
    46801 tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt gatggtaacc
    46861 gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggggatagc cgcgtaccag
    46921 aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg
    46981 atattgtttt cccgcaatat ggttttgccc aacataaagg gtatccaacc gcttttcatc
    47041 tggaaaaact ggctgaatat ggcgcgactg aacatcatcg tcgcagcttt gggcctgtca
    47101 aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac cggaatctga
    47161 agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg atgatcgatg
    47221 gcttggccaa aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg ccagcactgg
    47281 cgatcaccga tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg ggacatggcg
    47341 cagggattaa gcctatcgtc ggggcagatt ttaatgtcca gtgcgacctg ctgggtgatg
    47401 agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat ctggcgttgc
    47461 tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact
    47521 ggcttatcga attaaacgaa gggttgatcc ttctttccgg cgggcgcatg ggcgacgtcg
    47581 gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag
    47641 aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg gatgaagaaa
    47701 gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacta
    47761 acgatgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc
    47821 acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg cagcaatata
    47881 tgcgtagcga agaggagatg tgtgagctgt ttgccgacat cccagaagcc cttgccaaca
    47941 ccgttgagat cgccaaacgc tgtaacgtaa ctgtgcgtct tggtgaatac ttcctgccgc
    48001 agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca aaagaaggcc
    48061 tggaagagcg tctggcattt ttattccctg acgaggaaga acgtcttaaa cgccgcccgg
    48121 aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc ttcccgggct
    48181 acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta ccggtagggc
    48241 caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc accgacctcg
    48301 atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt gtctccatgc
    48361 ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgaa cacgtggcgg
    48421 acatgtacgg tcgtgatgcg gtatcgcaga ttatcacctt cggtacgatg gcggcgaaag
    48481 cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc gaccgtatct
    48541 cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa gccgagccgc
    48601 agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac atggcgcgca
    48661 aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt atcgcgccga
    48721 ccaaaattac cgattttgcg ccgctttatt gcgatgaaga gggcaaacat ccggtcaccc
    48781 agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc cttggtttgc
    48841 gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg
    48901 gcgagccacc gctggatatc gccgcgatcc cgctggatga caagaaaagc ttcgacatgc
    48961 tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg aaggacctga
    49021 tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca ctgttccgcc
    49081 ctggtccgtt gcagtcaggg atggtggata actttatcga ccgtaaacat ggtcgtgaag
    49141 agatctccta tccggacgtg cagtggcagc atgaaagcct gaaatcggta ctggagccaa
    49201 cctacggcat tatcttgtat caggaacagg tcatgcagat tgcccaggtg ctttctggtt
    49261 ataccctcgg tggcgcggat atgctgcgcc gtgcgatggg taagaaaaag ccggaagaga
    49321 tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac
    49381 tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt aacaaatcgc
    49441 actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg cactatccgg
    49501 cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag gtggtgggcc
    49561 tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata aactccggtc
    49621 tttaccattt ccacgtcaac gacgatggcg aaatcgtgta tggtattggc gccatcaaag
    49681 gggtaggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc ggctacttcc
    49741 gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg
    49801 aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga
    49861 actcgctggg tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa gctatcggtc
    49921 aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa tcctacgcca
    49981 gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg ttaggcctgt
    50041 acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat gtcggaggcg
    50101 taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct gcggggctcg
    50161 ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg
    50221 atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat aaataccagc
    50281 aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt gatgacttca
    50341 gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat
    50401 atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag cttttaaacc
    50461 gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat ctctactatc
    50521 agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg
    50581 atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa ctggagtttg
    50641 actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg attgcagagc
    50701 tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa ctggatatta
    50761 acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg cgtaaaatct
    50821 tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccgcag cgtccttata
    50881 ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc gaccgtgcgt
    50941 atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt ccggtgatga
    51001 tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc
    51061 cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa cgctttaaga
    51121 tgcctatcat cacctttatc gacaccccgg gggcttatcc tggcgtgggc gcagaagagc
    51181 gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctt ggcgtaccgg
    51241 tagtttgtac ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg
    51301 ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa ggttgtgcgt
    51361 ccattctgtg gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg
    51421 ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg ctgggcggcg
    51481 ctcaccgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgctg gcggatctgg
    51541 ccgatctcga tgtgttaagc actgaagatt taaaaaatcg tcgttatcag cgcctgatga
    51601 gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt ggcccttttt
    51661 tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg aacacatgaa
    51721 catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca tcaaagaact
    51781 ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca gcgttgattt
    51841 gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact gggatgagta
    51901 cagtctcgat ttatgcagcg atatcaatca acttaatgaa tatctcccgc tttatgcctt
    51961 catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg cgctctggtt
    52021 ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc agtacaccaa
    52081 cgaatatctt gataacatta cgccgccgtt cacgaaagcc ttgtttacct acgtcaaaga
    52141 gcggaagtac accttttgta cgccggggca tatgggcggc accgcatatc aaaaaagccc
    52201 ggttggctgt ctgttttatg attttttcgg cgggaatacc cttaaggctg atgtctctat
    52261 ttcggtcacc gagcttggtt cgttgcttga ccacaccggg ccacacctgg aagcggaaga
    52321 gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg gaatatcgac
    52381 gtcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt tgatcgaccg
    52441 caactgccat aaatcactgg cgcatctgtt gatgatgaac gatgtagtgc cagtctggct
    52501 gaaaccgacg cgtaatgcgt tggggattct gggggggatc ccgcgccgtg aatttactcg
    52561 cgacagcatc gaagagaaag tcgctgccac tacgcaagca caatggccgg ttcatgcggt
    52621 gatcaccaac tccacctatg atggtttgct ctacaacacc gactggatca aacagacgct
    52681 ggatgtcccg tcgatccact tcgattccgc ctgggtgccg tatactcatt ttcatccaat
    52741 ctaccagggt aaaagtggta tgagcggcga gcgtgttgcg ggaaaagttt tcttcgaaac
    52801 gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcactgatcc acattaaagg
    52861 cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca cctcgcccag
    52921 ttatcccatt gttgcttcgg ttaagacggc ggcggcgatg ctgcgtggta atccgggcaa
    52981 acggctgatt aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg tccagcggct
    53041 gcgggaagag tctgacggtt ggtttttcga tatctggcaa ccgccgcagg tggatgaagc
    53101 cgaatgcagg cccgttgcgc ctggcgaaca atggcacggc tttaacgatg cggatgccga
    53161 tcatatgttt ctcgatccgg ttaaagtcac tattttgaca ccggggatgg acgaacaggg
    53221 caatatgagt gaggagggga tcccggcggc gctggtagca aaattcctcg acgaacgtgg
    53281 gatcgtagta gagaaaaccg gcccttataa cctgctgttt ctctttagta ttggcatcga
    53341 taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa tttaagcgct cttacgatct
    53401 caacctgcgg atcaaaaata tgctgcccga tctctatgct gaagatcccg atttctaccg
    53461 caatatgcgt attcaggatc tggcgcaagg gatccataag ctgattcgta aacacgatct
    53521 ttccggtttg atgttgcggg cattcgatac tttgccggaa atgatcatga cgccacatca
    53581 ggcatggcag agacagatta aaggtgaagt agaaaccatt gcgctggaac aactggtcgg
    53641 tagagtatcg gcaaatatga tcctgcctta tccaccgggc gtaccgctgt tgatgccagg
    53701 agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc tttgttccgt
    53761 agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg acgaagacgg
    53821 cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg cttccgggcg
    53881 agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca ggttcaccat
    53941 attgcgatta ttgcgacgga ttatgcggtg agcaaagctt tctactgcga tattcttggt
    54001 ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg ggatttggcg
    54061 cttaatgggc aatatgtgat tgagcttttc tcttttccgt tcccgccgga gcgccccagc
    54121 cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgacat cgatgcggca
    54181 gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga tccatacacg
    54241 caaaaacgct tcaccttctt taacgatccg gacgggctgc cgttggaact gtatgagcag
    54301 taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac tgtaaaatcg
    54361 caccatcatg acactcacgc tcaatagaca acttctcacc tcacgccaga ttctggtggc
    54421 ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt ggcggacgga
    54481 aaatccgggc gtcactctgc gcgctattca tgtgcatcac ggtttaagtg ccaatgccga
    54541 tgcctgggtt acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc tggtggtcga
    54601 acgcgtacaa cttgcgcaag aggggctggg gattgaggcc caggcgcggc aggcacgtta
    54661 tcaggcattt gcccgtaccc tattgcccgg cgaagtgctg gtcaccgcgc aacatctcga
    54721 cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt agcggccctg ccgggctttc
    54781 ggctatggcg gaagtttcgg agtttgctgg aacacggctt atccgcccgt tgcttgcccg
    54841 cacgcggggg gaactggagc agtgggcatt ggcgcatggt ttacgctgga ttgaagacga
    54901 aagtaatcag gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag tgccgttatt
    54961 gcagcagcgt tggccgcatt ttgcggaagc aacggcccgc agcgccacac tttgtgctga
    55021 acaagagagt ctgttggatg aactgctggc ggatgattta gcacactgcc aaacgtcgca
    55081 ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgtcgcg cggcgattat
    55141 tcgccgctgg ctggcagggc agaatgcacc gatgccttcc cgcgacgcgc tggtgcgcat
    55201 ctggcaggaa gtggcgctgg cgcgggaaga tgcttccccc tgtttacgtt tgggcgcctt
    55261 tgaaatccgc cgctatcagt cgcaactgtg gtggattaaa tccgtcaccg ggcaaagcga
    55321 aaccattgtg ctgtggcaga cgtggctaca accgctggaa ttaccggcgg ggctgggaac
    55381 cgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag cggtcagcgt
    55441 gcgtttcaaa gcgccaggat tgctgcatat tgtcgggcgc aacggcggac gtaagctgaa
    55501 gaaaatctgg caagagttag gcgtgccgcc gtggctacgt gacaccacgc cactgctgtt
    55561 ttatggagaa acgctgattg cggcggcagg ggtatttgtg acgcaagaag gtgtggctga
    55621 aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga aagccggata
    55681 agacgcatca aacgtcgcat ccggcgaaag tcaatcagga ttcgcttacc accaccgtac
    55741 cgatttcagg gtggctaaag ctggtaattt tatccagacg cagctcgcgg gtttcgccag
    55801 cggcctcgac gaccagatac tccacatttt tgcgggagac taaatcactg gcttttgcct
    55861 gcaatttttc gccatctttc agctcaagtg tcagcattaa atgatgctgg caggcgagct
    55921 caagattatc gtaatcatca caattaattg gttgatacgt atcattcatt gacataatcg
    55981 ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc agaaagggcg
    56041 tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc cggaacgtat
    56101 cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca atatttttcc
    56161 acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc ctacgaagat
    56221 aaactctgtt tcgcaaggtg actataccac actcatttct gcaatatcag cgccgcaact
    56281 gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg gcgcttaaag
    56341 cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag tttctcgatg
    56401 cctctctgac gctggcgcgc cacccttcag aaacccagga gcgtatgatg ctgcgcttgc
    56461 tggcgtggct gaaatatgct gatgaacgtc tgcaatttac ccgtggtttg tgtgccgatg
    56521 atgagccgga agcgtggctg cgtaacgatc acctgggcat tgatttgtgg attgagctgg
    56581 ggctgccgga tgagcgacga attaagaaag cctgcaccca ggccgcagaa gtggcgctgt
    56641 ttacctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc aaatgtgtgc
    56701 agtttgccaa tctttccgtc tggtatctgg acgatgaaca actggcgaaa gtaagcgcct
    56761 ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggtgttatc tggttatcgg
    56821 atgataagaa taatctggaa gttaacttaa ccgcctggca acaaccttca tgattgtgat
    56881 ttcccgacat gttgctattc ccgatggtga gcttgagatc accgccattc gtgcgcaggg
    56941 cgctggcggg cagcatgtta ataagacctc aacggctatt catctgcgtt ttgacattcg
    57001 ggcgtccagc ctgccagagt attacaaaga gcgtctgctc gccgccagcc atcatttgat
    57061 cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcagg aactgaaccg
    57121 cgaagccgct ctggcccggc tggtggcagt gattaaagat ttaacaaaag aacaaaaagc
    57181 ccgacgaccc acgcggccca cccgtgcatc gaaagagcgc aggctggcat cgaaagcaca
    57241 aaaatcaagc gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat aaaaagaagg
    57301 aatggatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc tttactctga
    57361 tgggatgtaa taatcgggcc gaagtcgata cgctttctcc ggcgcaggct gccgaactga
    57421 aaccgatgcc gcaaagttgg cgcggcgtgc taccgtgtgc cgattgcgaa ggaatcgaaa
    57481 tctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat ctcggtgctc
    57541 gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct gacaagctgg
    57601 tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat gcgctggaga
    57661 tgctcgatcg tgaaggcaat ccaattgaat cgcagttcaa ctatacgctg gaaccggcac
    57721 aatccagttt acctatgacg ccgatgaccc tgcgaggcat gtatttttat atggctgatg
    57781 cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc
    57841 tggagcgtag ctacctggct gcgcgcggtc acagtgaaaa accggtgtta ctgtcagtag
    57901 aaggtcactt tacgcttgag gctaatccgg atactggtgc gccaaccaaa gtattagcgc
    57961 ccgatacggc gggtaaattt tacccgaata aggattgcag tagtttgggg cagtaaacct
    58021 gcttaaggca gaaacaaacg caaaattgcc tgatgcgctg cgcttatcag gcctacagca
    58081 tataatgcaa cattttgaat tagcacgatt ttgtaggccg gataaggcgt ttacgccgca
    58141 tccggcattt tgtgcacgag gctggcttta tcactgagtc agcccaacaa agcgacccgc
    58201 cttgatataa atcccaggct tcaggtgccc aacatactgt aactgcgttt ctggcttaaa
    58261 cgcagagaca tcaccaatac gcatatgcat cagatcggca gggctaatcc agcccgattg
    58321 cgccagtgta agtggatgcc cggatttggc aaatgcatca gtgacaaatt ccgaacaaaa
    58381 ccacgacttt ttgtctcctt cgcacacact gcttaactgc gctttcgcca ggtcgctgac
    58441 gcactgttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat
    58501 aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc
    58561 aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag
    58621 cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc
    58681 ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa
    58741 gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt
    58801 gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa
    58861 tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct
    58921 gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaagcaggc ggcagtacgc
    58981 ctttggttta tccattttat acaatccatg taaaaaaggg ccctgaaatt caggaccctt
    59041 tctggcatca gcctttaatc tgtttcacca gatattcgac gatgtcacca gtcttaatta
    59101 actgtttctc gccgttgcga cgatatttat attcgatatc gtcgttgtcg aggttacggt
    59161 cgcccagcac aatagtgtgc ggaataccga tcagttccat atcagcaaac atcacgcccg
    59221 gacgctcttt gcggtcatcc agcagcactt cgataccttg tgcacgcagt tcgctgtaca
    59281 gtttctcagc aagctcttgt acgcggaagg atttgtgcat gttcatcggc agaatcgcca
    59341 cctggaacgg cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag ttctgctcaa
    59401 tcgccgcagc taccacacgc gttaccccga taccgtagca acccatcgtc aggatttggt
    59461 tacggccatc ttcaccctgt acggaggctt tcagtgcttc ggagtacttg gtacccagct
    59521 ggaagatgtg accaacttcg ataccacgtt tgatcagcag cgtaccctgg ccatccgggc
    59581 ttggatcgcc agccaccacg ttacggatat ctgcaacttc cggggtagcg acatcgcgat
    59641 cccagttgat accgaagtag tgtttaccat cgatgttagc accagcagcg aaatcactca
    59701 tcgccgcaac ggtacggtca atcaccaccg gaatcggcat gtttaccgga cccagtgaac
    59761 ccggaccggc tttaaccaca gcacgaattt cttcttcggt cgcgaaagtc agcgggcttg
    59821 caacctgcgg cagtttttct gctttaactt cgttcagctc gtgatcaccg cgcaccagca
    59881 gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac cagcagagtc ttaaccgttt
    59941 tctcaatcgg cagattgaac tgttcaacca gttccgcgat ggttttcgcg ttcggcgtat
    60001 caaccagcgt catttcctgg gtagcagcag cgcgcggttc tttcggcgcg atagcttctg
    60061 ccagttcaat gttcgctgca tagtcagagg tgtcggagaa gaccacatcg tcttcaccgc
    60121 tctgcgccag cacctggaat tcgtgagagg cgctgccgcc gatagaaccg gtgtcggcct
    60181 gtacggcgcg gaaatccagc cccatgcggc tgaagatttt gctgtaggcc gcatacattg
    60241 catcgtaggt ttcctgcagg gattcctgag aagtatggaa agagtaagca tctttcatca
    60301 ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg aacttggtct
    60361 ggatctgata gaagttcagc ggcagctgtt tgtaagagct aagctcgtta cgaatcaggt
    60421 cagtgataac ttcttcatga gttgggccga gtacgaacgg acgctcgcca cggtcaacaa
    60481 aacgcagcag ttccggaccg tactgttccc aacgaccact ctcttgccac aaatcggctg
    60541 gctgaaccac cggcatcgac acctcgatcg caccggcgtt gttcatctct tcacgcacga
    60601 tgttttcgac ttttttcaga acgcgcacgc cggtcggcag ccaggtatat aacccggagg
    60661 ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg gctgatcacc tcggcgtcgg
    60721 caggtgtctc cttgagagtg gagagcaggt attggctagt acgcatgttg ttacggttcc
    60781 agttggaagg tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat ttagtttacc
    60841 agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac
    60901 ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata
    60961 ggttttgccc gtttcctcac ctttacgata ggccgggcgc gggtcctgcg ccagtacttc
    61021 gcggataaac agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac
    61081 ctctgcggta aaactcaccg ccatctctgc agctggcgca ctttgcgcat agctggcact
    61141 ggcatcggga agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc
    61201 atcgaccaga tccagactgc cgagcttcag aatcacgctg cctttatggc aaacaacctc
    61261 tttcagctct accagcgaca tgccaattgg gttagggcgg aaagtagagc gtgttgcgaa
    61321 aacccccatt ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc
    61381 ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt ccaggccgcg
    61441 aacggcgtcg gcctggttgt aggaagctat gagatgcagt tcgccgttgg cgctttttac
    61501 cagacctggc tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat
    61561 ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca
    61621 gcctgacgat agcagcccgg cgtaccgctg gtgacttcgc agctatgcag taatacagca
    61681 ttggctttca ttttagaggc attgatttgc atctgcttac gtgcggttgg aatgctcggc
    61741 ggagagtcct gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac
    61801 ggtttgccga ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct
    61861 ttcggttttg caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg
    61921 gatctgctta acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca
    61981 cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa
    62041 atgacaagac gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa
    62101 aattaccagc ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca
    62161 tcagactgat aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc
    62221 gttacgatca ggtttacgta tggggagtct ttatcttcga caaagatacc gtctttcgcc
    62281 ggagtcaggc caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg
    62341 tcgtccagag aacgcggcag ttgcggtgct tccagttcaa caattttcag attttttggg
    62401 ttctcaacaa catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag
    62461 cccacttttt gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca
    62521 acctgcgaac catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc
    62581 ggataaacaa aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga
    62641 tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg
    62701 tttggcagaa catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc
    62761 gcaactttct gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta
    62821 atgtggtttg gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg
    62881 gctcccactg ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat
    62941 ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcaaac
    63001 tgaattaaat aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgttg
    63061 tagccgatgt agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca
    63121 cccatcgcgg aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc
    63181 ggcaacgctt ccggtaacag cactttacag acgatctgca ttggcgtggc acccattgcg
    63241 cgggaagctt caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca
    63301 ataaacggtg ctgcaccaac ggttaacgga acaattgctg cctgcaaacc aatcgatgta
    63361 ccgacaataa cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa
    63421 cggaaaatgt tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc
    63481 cccggacgcg tgacataaag cagaacgcca accggcagac caatcacaaa cccaaaaaat
    63541 ccggatacga aggtcattgc cagcgtttcc catacgccac gaaccagcag ccacatcatc
    63601 ggctcagaca taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc
    63661 ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga atttaacgcc
    63721 accggcgtag tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt
    63781 ttcagaaagc agtggggcat cgaccgattg accggtaaac tccagacgca gcatcggcac
    63841 gcagtcagta aatggctccg tttgcagacg ttcctggtaa tcttccggga tatccagatg
    63901 cagggtcgac tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact
    63961 taccgtgtcc tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg
    64021 cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat
    64081 gtctttcagc agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc
    64141 atcacacagc aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt
    64201 ctgcccaccg gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga
    64261 cagcaattcc gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg
    64321 cagagccacg ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat
    64381 accaatctgg cggcgagctt tggtcaactc ggattctgac agcgtggtca attcctggcc
    64441 atcgaccagc acgctaccct cggttgggcg ctccagcaag tttacacaac gtataagcgt
    64501 actcttaccc gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag
    64561 gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt
    64621 cgaaagtttt atcattgatt atttattatc gtcattaagt tagtcgtagc atctcgaatg
    64681 cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa
    64741 tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcccatg
    64801 ccttattaag gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga
    64861 tggcaccatt aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga
    64921 cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac
    64981 caaccagtct ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga
    65041 gtggatggac tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc
    65101 gcatcatccg caggggagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca
    65161 tccggggatg cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat
    65221 ggtgggcgat aaattagaag atatgcaggc agcggttgcg gcgaacgtgg gaacaaaagt
    65281 gctggtgcgt acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt
    65341 aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cgcaatgatt
    65401 aaaagatgaa cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc
    65461 tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg
    65521 ttgaagagaa aaaatcctga aattcagggt tgactctgaa ataggaaagt gtgatatacg
    65581 ccacctcgcg acagtgagct gaaagctgcg tcgcaactgc tctttaacaa tttatcagac
    65641 aatctgtgtg ggcactcgaa gatacggatt cttaacgtcg caagacgata aatgaatacc
    65701 aagtctcaag agtgaacacg taattcatta cgaagtttaa ttctttgagc atcaaacttt
    65761 taaattgaag agtttgatca tggctcagat tgaacgctgg cggcaggcct aacacatgca
    65821 agtcgaacgg taacaggaag cgcttgctgc ttcgctgacg agtggcggac gggtgagtaa
    65881 tgtctgggaa actgcctgat ggagggggat aactactgga aacggtagct aataccgcat
    65941 aacgtcgcaa gaccaaagag ggggaccttc gggcctcttg ccatcggatg tgcccagatg
    66001 ggattagctt gttggtgggg taacggct
//
LOCUS       DAJTUI010000011        64183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTUI010000011 DAJTUI010000000
VERSION     DAJTUI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.011"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            121..564
                     /locus_tag="OO464_000764"
     CDS             121..564
                     /locus_tag="OO464_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6226021.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            800..1618
                     /gene="ybhA"
                     /locus_tag="OO464_000765"
     CDS             800..1618
                     /gene="ybhA"
                     /locus_tag="OO464_000765"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR6226022.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1619..2677)
                     /gene="modC"
                     /locus_tag="OO464_000766"
     CDS             complement(1619..2677)
                     /gene="modC"
                     /locus_tag="OO464_000766"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6226023.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2680..3369)
                     /gene="modB"
                     /locus_tag="OO464_000767"
     CDS             complement(2680..3369)
                     /gene="modB"
                     /locus_tag="OO464_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6226024.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3369..4142)
                     /gene="modA"
                     /locus_tag="OO464_000768"
     CDS             complement(3369..4142)
                     /gene="modA"
                     /locus_tag="OO464_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6226025.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4309..4458)
                     /gene="acrZ"
                     /locus_tag="OO464_000769"
     CDS             complement(4309..4458)
                     /gene="acrZ"
                     /locus_tag="OO464_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6226026.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4587..5375
                     /gene="modE"
                     /locus_tag="OO464_000770"
     CDS             4587..5375
                     /gene="modE"
                     /locus_tag="OO464_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6226027.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5443..6915
                     /gene="modF"
                     /locus_tag="OO464_000771"
     CDS             5443..6915
                     /gene="modF"
                     /locus_tag="OO464_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6226028.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7176..8192
                     /gene="galE"
                     /locus_tag="OO464_000772"
     CDS             7176..8192
                     /gene="galE"
                     /locus_tag="OO464_000772"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6226029.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8202..9248
                     /gene="galT"
                     /locus_tag="OO464_000773"
     CDS             8202..9248
                     /gene="galT"
                     /locus_tag="OO464_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6226030.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9252..10400
                     /gene="galK"
                     /locus_tag="OO464_000774"
     CDS             9252..10400
                     /gene="galK"
                     /locus_tag="OO464_000774"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6226031.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10394..11434
                     /gene="galM"
                     /locus_tag="OO464_000775"
     CDS             10394..11434
                     /gene="galM"
                     /locus_tag="OO464_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6226032.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11637..12389
                     /gene="gpmA"
                     /locus_tag="OO464_000776"
     CDS             11637..12389
                     /gene="gpmA"
                     /locus_tag="OO464_000776"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6226033.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12556..13608)
                     /gene="aroG"
                     /locus_tag="OO464_000777"
     CDS             complement(12556..13608)
                     /gene="aroG"
                     /locus_tag="OO464_000777"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6226034.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13924..14304
                     /locus_tag="OO464_000778"
     CDS             13924..14304
                     /locus_tag="OO464_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6226035.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14418..15359
                     /gene="zitB"
                     /locus_tag="OO464_000779"
     CDS             14418..15359
                     /gene="zitB"
                     /locus_tag="OO464_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6226036.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15356..16075)
                     /gene="pnuC"
                     /locus_tag="OO464_000780"
     CDS             complement(15356..16075)
                     /gene="pnuC"
                     /locus_tag="OO464_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6226037.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16113..17156)
                     /gene="nadA"
                     /locus_tag="OO464_000781"
     CDS             complement(16113..17156)
                     /gene="nadA"
                     /locus_tag="OO464_000781"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6226038.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17405..17480)
                     /locus_tag="OO464_000782"
     tRNA            complement(17405..17480)
                     /locus_tag="OO464_000782"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17445..17447),aa:Lys,seq:ttt)
     gene            complement(17484..17559)
                     /locus_tag="OO464_000783"
     tRNA            complement(17484..17559)
                     /locus_tag="OO464_000783"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17524..17526),aa:Val,seq:tac)
     gene            complement(17611..17686)
                     /locus_tag="OO464_000784"
     tRNA            complement(17611..17686)
                     /locus_tag="OO464_000784"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17651..17653),aa:Lys,seq:ttt)
     gene            complement(17689..17764)
                     /locus_tag="OO464_000785"
     tRNA            complement(17689..17764)
                     /locus_tag="OO464_000785"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17729..17731),aa:Val,seq:tac)
     gene            complement(17900..17975)
                     /locus_tag="OO464_000786"
     tRNA            complement(17900..17975)
                     /locus_tag="OO464_000786"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17940..17942),aa:Lys,seq:ttt)
     gene            complement(18140..18931)
                     /gene="cpoB"
                     /locus_tag="OO464_000787"
     CDS             complement(18140..18931)
                     /gene="cpoB"
                     /locus_tag="OO464_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6226039.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18941..19462)
                     /gene="pal"
                     /locus_tag="OO464_000788"
     CDS             complement(18941..19462)
                     /gene="pal"
                     /locus_tag="OO464_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6226040.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19497..20789)
                     /gene="tolB"
                     /locus_tag="OO464_000789"
     CDS             complement(19497..20789)
                     /gene="tolB"
                     /locus_tag="OO464_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6226041.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20922..22163)
                     /gene="tolA"
                     /locus_tag="OO464_000790"
     CDS             complement(20922..22163)
                     /gene="tolA"
                     /locus_tag="OO464_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6226042.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22228..22656)
                     /gene="tolR"
                     /locus_tag="OO464_000791"
     CDS             complement(22228..22656)
                     /gene="tolR"
                     /locus_tag="OO464_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6226043.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22660..23352)
                     /gene="tolQ"
                     /locus_tag="OO464_000792"
     CDS             complement(22660..23352)
                     /gene="tolQ"
                     /locus_tag="OO464_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6226044.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23349..23753)
                     /gene="ybgC"
                     /locus_tag="OO464_000793"
     CDS             complement(23349..23753)
                     /gene="ybgC"
                     /locus_tag="OO464_000793"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6226045.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23903..24196)
                     /gene="ybgE"
                     /locus_tag="OO464_000794"
     CDS             complement(23903..24196)
                     /gene="ybgE"
                     /locus_tag="OO464_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6226046.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24196..24309)
                     /gene="cydX"
                     /locus_tag="OO464_000795"
     CDS             complement(24196..24309)
                     /gene="cydX"
                     /locus_tag="OO464_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6226047.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24324..25463)
                     /gene="cydB"
                     /locus_tag="OO464_000796"
     CDS             complement(24324..25463)
                     /gene="cydB"
                     /locus_tag="OO464_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6226048.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25479..27047)
                     /gene="cydA"
                     /locus_tag="OO464_000797"
     CDS             complement(25479..27047)
                     /gene="cydA"
                     /locus_tag="OO464_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6226049.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27896..30529)
                     /gene="mngB"
                     /locus_tag="OO464_000798"
     CDS             complement(27896..30529)
                     /gene="mngB"
                     /locus_tag="OO464_000798"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR6226050.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30547..32463)
                     /gene="mngA"
                     /locus_tag="OO464_000799"
     CDS             complement(30547..32463)
                     /gene="mngA"
                     /locus_tag="OO464_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6226051.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32633..33355
                     /gene="mngR"
                     /locus_tag="OO464_000800"
     CDS             32633..33355
                     /gene="mngR"
                     /locus_tag="OO464_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6226052.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33458..34327)
                     /gene="sucD"
                     /locus_tag="OO464_000801"
     CDS             complement(33458..34327)
                     /gene="sucD"
                     /locus_tag="OO464_000801"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6226053.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34327..35493)
                     /gene="sucC"
                     /locus_tag="OO464_000802"
     CDS             complement(34327..35493)
                     /gene="sucC"
                     /locus_tag="OO464_000802"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6226054.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35587..36804)
                     /gene="odhB"
                     /locus_tag="OO464_000803"
     CDS             complement(35587..36804)
                     /gene="odhB"
                     /locus_tag="OO464_000803"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6226055.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36819..39620)
                     /gene="sucA"
                     /locus_tag="OO464_000804"
     CDS             complement(36819..39620)
                     /gene="sucA"
                     /locus_tag="OO464_000804"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6226056.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39921..40637)
                     /gene="sdhB"
                     /locus_tag="OO464_000805"
     CDS             complement(39921..40637)
                     /gene="sdhB"
                     /locus_tag="OO464_000805"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6226057.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40653..42419)
                     /gene="sdhA"
                     /locus_tag="OO464_000806"
     CDS             complement(40653..42419)
                     /gene="sdhA"
                     /locus_tag="OO464_000806"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6226058.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42419..42766)
                     /gene="sdhD"
                     /locus_tag="OO464_000807"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42419..42766)
                     /gene="sdhD"
                     /locus_tag="OO464_000807"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6226059.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42760..43164)
                     /gene="sdhC"
                     /locus_tag="OO464_000808"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42760..43164)
                     /gene="sdhC"
                     /locus_tag="OO464_000808"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6226060.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43545..43652
                     /locus_tag="OO464_000809"
     CDS             43545..43652
                     /locus_tag="OO464_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226061.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43858..45141
                     /gene="gltA"
                     /locus_tag="OO464_000810"
     CDS             43858..45141
                     /gene="gltA"
                     /locus_tag="OO464_000810"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6226062.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45532..46098
                     /locus_tag="OO464_000811"
     CDS             45532..46098
                     /locus_tag="OO464_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6226063.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46149..48608
                     /locus_tag="OO464_000812"
     CDS             46149..48608
                     /locus_tag="OO464_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6226064.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48623..49354
                     /locus_tag="OO464_000813"
     CDS             48623..49354
                     /locus_tag="OO464_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6226065.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49351..50427
                     /locus_tag="OO464_000814"
                     /pseudo
     CDS             49351..50427
                     /locus_tag="OO464_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50579..51625
                     /locus_tag="OO464_000815"
     CDS             50579..51625
                     /locus_tag="OO464_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6226066.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51622..52413)
                     /gene="nei"
                     /locus_tag="OO464_000816"
     CDS             complement(51622..52413)
                     /gene="nei"
                     /locus_tag="OO464_000816"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6226067.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52449..53183)
                     /gene="pxpA"
                     /locus_tag="OO464_000817"
     CDS             complement(52449..53183)
                     /gene="pxpA"
                     /locus_tag="OO464_000817"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6226068.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53173..54105)
                     /gene="pxpC"
                     /locus_tag="OO464_000818"
                     /pseudo
     CDS             complement(53173..54105)
                     /gene="pxpC"
                     /locus_tag="OO464_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54099..54755)
                     /gene="pxpB"
                     /locus_tag="OO464_000819"
     CDS             complement(54099..54755)
                     /gene="pxpB"
                     /locus_tag="OO464_000819"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6226069.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54778..55521)
                     /gene="ybgI"
                     /locus_tag="OO464_000820"
     CDS             complement(54778..55521)
                     /gene="ybgI"
                     /locus_tag="OO464_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6226070.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55792..57273
                     /gene="dtpD"
                     /locus_tag="OO464_000821"
                     /pseudo
     CDS             55792..57273
                     /gene="dtpD"
                     /locus_tag="OO464_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
     gene            complement(57423..58841)
                     /gene="phrB"
                     /locus_tag="OO464_000822"
     CDS             complement(57423..58841)
                     /gene="phrB"
                     /locus_tag="OO464_000822"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6226071.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58838..59347)
                     /locus_tag="OO464_000823"
     CDS             complement(58838..59347)
                     /locus_tag="OO464_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6226072.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59476..59763
                     /locus_tag="OO464_000824"
     CDS             59476..59763
                     /locus_tag="OO464_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR6226073.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59818..60198)
                     /locus_tag="OO464_000825"
     CDS             complement(59818..60198)
                     /locus_tag="OO464_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226074.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60201..63587)
                     /locus_tag="OO464_000826"
     CDS             complement(60201..63587)
                     /locus_tag="OO464_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6226075.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(63606..63809)
                     /locus_tag="OO464_000827"
                     /pseudo
     CDS             complement(63606..63809)
                     /locus_tag="OO464_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15690 a        17273 c        15617 g        15603 t
ORIGIN      
        1 gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg actgttaatt
       61 gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt cggcctttgc
      121 atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat gagatgggag
      181 gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa aattcgcatt
      241 tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact ggataacttg
      301 aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag gacaattgca
      361 gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga cccaccgcca
      421 tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg cagtgatttc
      481 actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg tgacggacga
      541 gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcaaaatg ccacttgtaa
      601 ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg ctcctttgca
      661 tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg ctgaattaaa
      721 acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga aaattcttaa
      781 ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag acggcacctt
      841 attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc gcgctcgcga
      901 agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc atccttttta
      961 tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt tgtatgatta
     1021 tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc tgcaactcat
     1081 tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg caatggtcta
     1141 tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc tgccgccgga
     1201 acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac aagttaacgc
     1261 cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt ttggtaagca
     1321 tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg ttgatattgc
     1381 acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc aaggttggtc
     1441 gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc tggaagccgc
     1501 tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg ccaacattgt
     1561 gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc tgatttaatc
     1621 aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt ttgatcgcca
     1681 gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg acttccagct
     1741 ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc aatacgttac
     1801 gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga
     1861 tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc tgatcgccca
     1921 gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact ttcagaatgc
     1981 tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac acttcctcca
     2041 gagcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct gccagatgga
     2101 ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc cgtgtcagcc
     2161 gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc agcggttcat
     2221 ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg cgctgttttt
     2281 cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca
     2341 ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga ttgccacgca
     2401 ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga cgcttttccg
     2461 gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg actatccgcc
     2521 ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt ccggcaccgg
     2581 agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg gtcaggcaat
     2641 ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg cccgttcacg
     2701 gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg caatagaaat
     2761 aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg tatacatggc
     2821 agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg ttgcaccaaa
     2881 ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg gtaaggtcag
     2941 cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg
     3001 ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca tcagcggaaa
     3061 cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa
     3121 ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta ataataagta
     3181 accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga gcagagcttt
     3241 gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa
     3301 cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt ctggatcggt
     3361 cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc
     3421 ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac aacagcaacc
     3481 ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt tacccctttg
     3541 ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg ttcgaccagc
     3601 gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt atcccatgcg
     3661 cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc cggatcgcca
     3721 accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc gatggtgaaa
     3781 tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag cagtgtctga
     3841 cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt ctgatcggca
     3901 gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt agatgacgaa
     3961 gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc aatgtcctgc
     4021 attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc tgccagtgca
     4081 ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca tttacgagcc
     4141 attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa ctcggaattt
     4201 tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg caggaaaagc
     4261 aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc aatgattttg
     4321 tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac caagaccgta
     4381 aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga ataccagact
     4441 ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc taaccgctaa
     4501 gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac
     4561 aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc cttaagctcc
     4621 aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt gcgctttccg
     4681 gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc tgggatgcca
     4741 ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca ggcggtaaag
     4801 gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc tatgacttac
     4861 tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc ctgccgctga
     4921 acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt aaccagtggt
     4981 tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat gtcttactgg
     5041 ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc
     5101 tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc attactcagg
     5161 acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt catattgagc
     5221 gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca
     5281 cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg gcctacttta
     5341 atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt gttatgaaac
     5401 acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc gttgcaaatt
     5461 ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca gctaacgtta
     5521 aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc ggcgctggcc
     5581 cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca gttttcccac
     5641 atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg gcaacggaat
     5701 aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc tgagattatt
     5761 caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt cggtattacc
     5821 gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa aaccctgctg
     5881 tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt cgatggcctg
     5941 gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca gtccggtatt
     6001 actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca gtttgctggc
     6061 gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct ccagcaagca
     6121 ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc ggagccggat
     6181 gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct gaacaatggc
     6241 gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt gaatccaggc
     6301 gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt aagcctgatt
     6361 actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg acgtcgcggc
     6421 agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag tagtttgcat
     6481 ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta ttttgattcg
     6541 attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca gtggctggat
     6601 attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc ctggggacag
     6661 cagcgtttgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct tattctcgat
     6721 gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt tgttgatgtg
     6781 ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga agatgcgcct
     6841 gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg ctatgtgctg
     6901 acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag cgcactctcc
     6961 ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata aaaagcgcaa
     7021 tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg gctaaattct
     7081 tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt gttatgctat
     7141 ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt accggtggta
     7201 gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat gatgtcatca
     7261 ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg
     7321 gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg accgagatcc
     7381 tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc gtcgccgaat
     7441 cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg cgcctgatta
     7501 gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc accgtttatg
     7561 gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg caaagccctt
     7621 atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg
     7681 actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg tcgggcgata
     7741 tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc caagttgctg
     7801 taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa gatggtactg
     7861 gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa
     7921 aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta ggcagcagcg
     7981 tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac tatcattttg
     8041 caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa gccgaccgtg
     8101 aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc tggcactggc
     8161 agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa tttaatcccg
     8221 ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg gtttcaccgc
     8281 accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag gtgttacctg
     8341 cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc
     8401 ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg tctgacacgc
     8461 cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc
     8521 gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc gttgcggcat
     8581 tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt
     8641 gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg catccgcacg
     8701 gacagatttg ggcaaatagc ttcctgccta acgaagctga gtgcgaagac cgcctgcaaa
     8761 aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg
     8821 cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg ccttactggg
     8881 ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg atcaccgatt
     8941 tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc agtcgttatg
     9001 acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca ccatttaatg
     9061 gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg ctgcgctccg
     9121 ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag cgagacctga
     9181 ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg
     9241 gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct
     9301 accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg
     9361 actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg
     9421 caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat cagctcgacg
     9481 agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg aactacgttc
     9541 gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg gacatggtga
     9601 tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg
     9661 tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa atcgcgctta
     9721 acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg gatcagctaa
     9781 tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca ctggggacta
     9841 aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta
     9901 ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct
     9961 tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt gcacatgagc
    10021 tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg
    10081 aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg atggccgagt
    10141 ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag
    10201 aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg
    10261 gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag caagctgtcg
    10321 ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac
    10381 aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc agccgtaccg
    10441 actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact ggggtgcgac
    10501 tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg
    10561 tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta ttggtcgtta
    10621 tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga cgctttcgcc
    10681 aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca
    10741 gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg atggcgatca
    10801 gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat
    10861 ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga ctaatcacgt
    10921 ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc agattctggc
    10981 ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga aatctgtcgc
    11041 cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga
    11101 cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca aaggcgatgg
    11161 caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga aggtctacac
    11221 caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac cgtcgcgggg
    11281 aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc tgccggacag
    11341 cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc
    11401 cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca tgagggcttt
    11461 atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca atattcacaa
    11521 caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc tgttgcttcg
    11581 tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt aagcatatgg
    11641 ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt
    11701 tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca aaagcagcag
    11761 gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct gtgctgaaac
    11821 gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg
    11881 agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg
    11941 aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt tttgcagtga
    12001 ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg cgttacgcga
    12061 aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt gaccgcgtga
    12121 tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc gtaatcatcg
    12181 ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg agcgaagaag
    12241 agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc gacgagaatt
    12301 tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg aaagcagcag
    12361 ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg agaaagccga
    12421 ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat tgcctgatgc
    12481 gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc tgacggcgca
    12541 tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt gacgtaacag
    12601 agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt aggccagcgg
    12661 ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca tcacgccaat
    12721 aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat ccatctgctt
    12781 tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag ctggcagacc
    12841 tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt taggctcttt
    12901 accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa tcgccgaatg
    12961 cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa tggcatcgat
    13021 agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag aaagaccaga
    13081 cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc cccagctcat
    13141 caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg ctggcagacc
    13201 gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt tgatctggaa
    13261 gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg tacgcggctt
    13321 ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac gcagcgccag
    13381 caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg ggccaatcac
    13441 caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc gggcatgggc
    13501 aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga caggaggaag
    13561 taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt ctgttccagt
    13621 gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg tcagaatgtg
    13681 taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa aacgccagat
    13741 tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg tttgttaatt
    13801 atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca atgccttata
    13861 ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata acaggagcat
    13921 cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag ccttgccagc
    13981 ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc aaacgccgca
    14041 gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa taacgtcgac
    14101 aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg ttcttcaatg
    14161 aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa agatggtcga
    14221 tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga tgtcgatacc
    14281 aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag agcggagtct
    14341 ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa gtgagaataa
    14401 ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac ctgccagaag
    14461 ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt atgctggtag
    14521 aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg ggtcatatgt
    14581 tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca cgtcgtcctc
    14641 ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg tttgtgaatg
    14701 ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa cgcttccgca
    14761 cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg ttgctggcaa
    14821 atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag
    14881 cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt attgccgcgt
    14941 tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata ctggtgtcgc
    15001 ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa ttacttgaag
    15061 gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg
    15121 aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg gtgatgacgt
    15181 tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag atccaacact
    15241 acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat caaccttgtc
    15301 ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat caccattaat
    15361 gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg ctgccgttga
    15421 gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc tgaagtgcaa
    15481 aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc acatacttac
    15541 gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc cagaacggga
    15601 aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc atgatcatga
    15661 ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc agaccaatcg
    15721 aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag cgaattttca
    15781 actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa atattcgcgg
    15841 caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag aaaataatgc
    15901 caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc agactggcaa
    15961 ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag agatcataac
    16021 cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa tccatctttc
    16081 ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc cgcaaaatcc
    16141 agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac atgaacctcg
    16201 tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt catggccatc
    16261 cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg tgcttccagt
    16321 aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc ggttgccaca
    16381 ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt ggaaccgacc
    16441 gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat ggcagcatcc
    16501 gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg cacaatgcag
    16561 gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg ccccagatgt
    16621 ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat aagttcgacg
    16681 gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga agtgttggcg
    16741 tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc ttcaacaggg
    16801 cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt tttttccgga
    16861 ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa agtagaagcg
    16921 ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc accggtttct
    16981 tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac cgcattacgt
    17041 tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat gcttaacggc
    17101 gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac gctcatctta
    17161 ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc gctgttatcg
    17221 gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag tcactcgaat
    17281 tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga tgtcatcagg
    17341 tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg gcgctttttt
    17401 gcattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct
    17461 accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc cgaccccctc
    17521 cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct ggatactaca
    17581 aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc gaacctgcga
    17641 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg gtgggtgatg
    17701 acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc
    17761 acccgacact acgctggata ctacaactaa ctatgttagt gggggtcgtg caggatgact
    17821 cgacttcgtc tcgcccttcg gcgccgttgc tgatgcaacg ttatccttca cgttttacta
    17881 ccgcttccac cttagcgatt ggtgggtcgt gcaggattcg aacctgcgac caattgatta
    17941 aaagtcaact gctctaccaa ctgagctaac gacccacttt ttcgttgctt tcggtttgtt
    18001 tgatatcccg tggcaacggc ggcatatatt actgatttca gacttgagcg caacaaaaat
    18061 ttcgatgcag atcactcaac tgcttatgat tcgcacgaca cgaccagaaa taatgcgact
    18121 tctggtcgtg tgttatgcat tacatcgcgt tcagacgttt ttgtgcctgt ttagcgccat
    18181 cagtaccagg gtatttactg ataacctgct ggtatacggc tttcgctttt gcggtgtcac
    18241 ctttgtcctg catgatgacg ccgactttaa acatcgcatc agcagccttt ggtgacttcg
    18301 gatagttttt cactaccgaa gcaaaatagt acgccgcatc atctttttta cccttgttgt
    18361 agtttaactg acccagccaa taattggcgt ttggcaggta agttgaatca gggtaatttt
    18421 tgatgaaatt ctgaaatgcc accattgcgt catcctggcg ggatttatcc tgcaccagcg
    18481 caatagctgc attgtaatcc gtgtttgcat cgccgctttt caccggcgcg ccagcattcg
    18541 cagtaccagc atcagctgtc ggcgtcgttg atgccgtcgc accgctttga tcgctgctgg
    18601 ttgattgcgc cgctgcacca ccgctgctga ggctgtcgat ctgcaacagg atctgctttt
    18661 gccgctccac gacctgattc agttgatact gattttcctg aatctgacca cgcagggaat
    18721 caatatcgga ttgattatca gaaagttgtt gctggagttg ggttaaaagc tggctgtgag
    18781 cattagaaat acgctcaagt tgagtgacgc ggtcttcgac cgagcctgag ctgacactac
    18841 tgattggtgc ctgagcaaaa gcggcccagg gggccgctat accaaccagt aacgacagac
    18901 tcaatagttg atgtctgaag ttactgctca tgcaattctc ttagtaaacc agtaccgcac
    18961 gacggttttt ggagtatgcc gcttcgtcat gacccagtac tgcaggtttt tctttaccgt
    19021 aagaaacgat ggagatctgg tctgcagaaa cgcctttacc ctgcaggtac atcttaacgg
    19081 cgttcgcacg acgttcaccc agggagatgt tgtattccgg agtaccacgt tcgtccgcgt
    19141 gaccttctac ggtgactttg taagacgggt tgctacgcag gaagtttgca tgtgcatcca
    19201 gcatttgagc gaagtcagaa cggatatcgt acttgtccag atcgaagtaa acgatgttgt
    19261 tctgctgcag ctgttgcatt tgcagacgag cctgctcttc ggaagacatg ttgccgttgc
    19321 cgccgttcgc atccatacca gtgccggcac ccagcatgcc ttcgctgccg tcattgctgg
    19381 cgttcttgtt ggaagaacat gccgcaattg ccataacagg cagagcaatc atcagccctt
    19441 tcagcacttt gttcagttgc atttcaatga ttcctttact attcaattaa ttattatcac
    19501 agatacggcg accaggcagg gaatttgacc tgtccatcag ttgccggaag acgcgctttg
    19561 aaacgcccat ctgtagaaac caaattcagc acggatccca tcccctgaga agagctgtag
    19621 attaccatag tgccgttagg tgccagactt ggcgtttcat ccaggaacgt ggacgacaga
    19681 acttgtacgc ctcccgttgc cagatcttgt ttggcaatgt gctgctgccc accattggag
    19741 ctgaccatta ccataaattt accgtcgctg ctgacatccg catcctggtt ctgcgaacct
    19801 tcccaggtaa tacgttgtgg cgcaccgccg ttgatattca ctttataaac ctgcggacga
    19861 ccggcctggt cagaagtaaa tgccaggttc tggctatccg ggaaccaggt cggttcggta
    19921 ttgttactgc gaccatcagt cacctggcgg atctgaccag aagccaaatc cattacgtac
    19981 aggttcagac taccggtttt cgacaaggcg aatgccagtt tgctgccgtc tggcgagaat
    20041 gcaggtgcac cgttgtgacg cgggaatgaa gccacctgac gtacagcgcc atttgccagc
    20101 gtctgaataa ccagcgcgga acgaccgctt tcgaaggtca cataagccag tttagaaccg
    20161 tctggtgacc acgccggaga catcagcggc tgcggtgaac ggtgaacgac aaactggttg
    20221 taaccgtcat agtcagatac gcgcagttca tacgggaact gaccgccgtt ggtctgaaca
    20281 acgtaggcaa tacgggtacg gaacgcacct ttaatgccgg tcagtttttc aaacacttca
    20341 tcactggcgg tatgaccagc ataacgcagc cactgcttgt tcactttgta cgagttctga
    20401 gcaagtacag tacccggtgc gccgccagtg tcaacaagtt gataagcaac attgtaagaa
    20461 ccatccggat tcggagtgac ttgaccgaca actacagcgt caatgcccag tgcggaccat
    20521 gcagctggtt gtacttcctg cgcactaccc ggctgctgtg gcagacgagc gcgatctaac
    20581 ggattaaatt taccgctgtt acgcaagtca gcagcaacga tgccgccaat atcttcaggt
    20641 gccgcaccag gccccgccca ctggaaagga acaacaccaa taggacgacc ggaatctaca
    20701 ccgctgtcga tcacaatgcg gacttcagca tgcagaactg atgcccacag tatgagaaaa
    20761 ccaaatgcta ctcgtaatgc ctgcttcatc atatctccct tatctggacc gatggcccac
    20821 gataatttag cagaatgtta acaaactaaa atacacaaaa ctaccagaac cccgtggcaa
    20881 ccggtgcctg atgttgaccg tccgaacagt caacatcgcg attacggttt gaagtccaat
    20941 ggcgcgtttt tgaacacttc atatactgcc tggcttggtg gtttcgggat cttcgcaagt
    21001 ttagctgctg ccaacgcagc ctgacaaagt gcgggatcgc caccttcagg tttgatatcc
    21061 agtaacatac catcgggtgc cagttttatg cgcagcgtac aggttttgcc tgcataggac
    21121 gatgcgtcat agaacttact ttcgatagca gatttaatct gcccggcata gttattgata
    21181 tcggcccctg atgcgccatt gtttttagta ttaccactcc cggcaggcga agcattgttc
    21241 cctttcgccc ctccccccgt tttcggtgca ttcttaccag agcttagctc accgaaaata
    21301 tcatctgcct ctgcggcagc ttttgctgca gcggcttttt cagctgctgc ttttgccgct
    21361 gccgcttttt tgtctgcggc ggctttttct gccgctgctt tcttatcagc tgctgcctta
    21421 tcagcagccg ctttttctgc tgcttttttg tcggctgcag ctttctctgc tgccgctttc
    21481 ttatcagctg cagccttttc agcagccgct ttcttctcag cttctgcctt ggctttttca
    21541 gcagcttcag ttgccgcttt ctttcttgct tcagctgcag ctgcttctgc cgcttccgct
    21601 ttcttcttca gtgccgcagc ggctacctcg gcttttttct gcgcttcggc tgcggctttg
    21661 gcggcttctg cttctgcttt tttcttcgcg tctgcagccg ctttcttcgc tgcttcttcc
    21721 gcagctttag catctgccgc cgctttcgct gccgccactt cagcttgctt ctgctttaac
    21781 tcggcctgtt ttgcggcttc ttcagcctgc tttttctgct cctgagccgc taaccgctct
    21841 ttctcaagtt gcttcaggcg ttcctgttca gccgcttgtt tctcacgcag ttcttcagca
    21901 gcctgctgtt ccttcatctt gcgctgttca tcagaacgct tcgcgcttga ttcctggctt
    21961 tgcatgcgtt tgtactgctc aactaccgca cctgaatcaa ccatgacagc gtcgatggac
    22021 gaaccaccgc cgcctccggc tgaagcttct atattctcat cgaacgaact ccagatcagc
    22081 gccgcaaata agatgacatg cagcactgct gaaataatta tcgcccgctt gagcttgtcg
    22141 ttttgttcgg ttgcctttga cactctcggt ttccaaaaac tgttcgcctg ttacccgctc
    22201 tctttcaagc aagggaaacg cagatgttta gataggctgc gtcattaaac caaccgattt
    22261 cacacccgca ctatgtaaca agttcagtgc tttaattatt tcatcgtaag gcacatcttt
    22321 tgcgccaccg atcagaaaga ccgttttcgg gttggccttg aaacggctgg acacttccgc
    22381 caccacctgc tctggtggta aacgctccag gcgatctttc tcaaccacca cggtgtactg
    22441 accaatacca gacacttcaa caatcactgg cggattatcg ttactgctca ccgcctgtga
    22501 ttcagtagcg tctggcagat cgacctccac gctctgggtg atgatgggcg ctgtcgccat
    22561 aaagatcagc aacagcacca gcagtacgtc cagcaacgga acaatgttga tttcggactt
    22621 gagatcgcga cgacctcgtc cacgcgctct ggccatggct taccccttgt tgctctcgct
    22681 aacggtaaac gcctggcggt gcagaatcgc ggtaaactct tccataaagt tgtcgtaatt
    22741 cagttccagt ttgtttacgc gctggttgag acggttataa gccattaccg ccggaattgc
    22801 ggcgaacaga ccaattgccg tcgcaatcaa cgcttctgcg ataccgggcg caaccatttg
    22861 cagtgttgct tgttttaccg ccccgagggc gataaaggcg tgcatgatcc cccagaccgt
    22921 accaaacaga ccaatatacg ggctgatgga gccaaccgta ccgaggaacg gaatgtgcgt
    22981 ttccagattt tcaagttcac ggttcatgga gatacgcata gcacgcgacg ccccttccac
    23041 tacggcttcc ggcgcatggc tattggcacg atgcaggcgc acaaactctt tgaacccgct
    23101 gtaaaagatt tgttccgaac cagtcagatt atcccgtttc ccctgactct cttgatagag
    23161 gcgagagagt tcgattccag accagaattt atcttcaaac gcttcggctt cacgcgccgc
    23221 tgcgttaaga atacgggttc gctggataat aatggcccag gatgcgattg aaaaaccaat
    23281 caaaatcaac atgataagtt taaccagaag gctagccttc aggaacaaat caaggatatt
    23341 catgtcagtc actgcttaaa ctccgcgaca atagacttgg gaagcgcacg aggcttcatt
    23401 ttgagtgggt caacgcaaac aaccagaacc tctgcttcat tcagcaaagt attctcggcg
    23461 ttgacaatac gttgcgtgaa aaccaaagag gtgccacgca ttgatgttat ttcagtctgt
    23521 atttcgagca tatcgtcgag ccgcgcaggt gcgtaatatt ccaccgtcat tttacgtacc
    23581 acaaaggcaa cgcgttcagc catcagcgcc tgctgactga agtgatgatg acgcagcatc
    23641 tctgtgcgtg ctctttcata aaaagcgacg taactggcgt ggtacaccac accaccggca
    23701 tcggtatctt catagtagac gcgaaccggc catcgaaaca gcgttgtatt cactttacat
    23761 cccggtaatg caacaaaagt tagagctttt aaacgctgct actatacgcg cgcgattgag
    23821 gtttgggaat gagggcaagt taagggcgcg taaaaattta tgggcctaga gcggcccata
    23881 attttttaaa gagaattctg atttaaaaga agaagaaaat cagcccgaca atgagaacaa
    23941 tatcggcaag caatgggcaa aaaatccctt gccaaagaac cttctgcgga cgaaagccca
    24001 cgccgtgaat cacaccagca cagacggccc acatcagcaa taaaccatgc cagatttcca
    24061 gttcactggt cttcgcggca aaacgagatg ggtcccaaaa catacatcct gctaacagaa
    24121 gcgccatcac gaaggaaagc gcccgcaggg ggcgcttgtc cattaccgca tacaaggtcg
    24181 cgataatctt actcatcaga tgtcttcttg accggctttg cctgattcga cgtgttcaag
    24241 cgccagcgcg gtgattaccc caaacgaaca ggcaagaagc gttcccagaa tccatgcgaa
    24301 ataccacatt tttagctcct tacttagtac agagagtggg tgttacgttc aatatcttct
    24361 ttggtgatac gaccgaacat tttccagtaa caccaggcgg tgtagagcag aatgatcggt
    24421 accagaacca ccgcaaccca ggtcatgacg ttaagcgtca gctggctgga agttgcatcc
    24481 cacattgtca gacttgcgtt catcatggtg ctggacggca tcacaaacgg gaacattgcg
    24541 ataccggctg tcaggatgat gcaggccagc gtcagggagg agaacacaaa cgcccacgcg
    24601 gctttatcca tacgtgcagt caggatggtc agcagcagca gaaccacacc cagtgccgga
    24661 atagcccaca gaattggcgt gttgttgaag ttaaccagcc atgcgccagc ttcacgaacc
    24721 acttctttat tcagtgggtt agaggctgcg taatggtcca ttgtcgattt cacgacataa
    24781 ccatcgatac cgtacatcac ccatacgcca gccagtgcga aacagaccag tgtcaccagc
    24841 gcagccacct gagccgttgc acgggtacgc aggtgcagtt cgcccacggt acgcatttgc
    24901 agataggttg cgccctgagt aatgatcatc cctacgctca ccacgcctgc cagcaggccg
    24961 aacgggttaa gcaactggaa gaagttaccg gtgtagtaca gacgcagata ttcatcaacg
    25021 ttgaacggta cgccctgcaa caggttaccg aacgctacac caattaccag cggcggaacg
    25081 aagctaccaa tgaagatgcc ccagtcccac atgttacgcc agcgggtttc ttcaatcttg
    25141 gagcggtagt caaaaccgac cggacggaag aacaaagacg ccagcacgag gatcatcgcc
    25201 acatagaagc cggagaacgc agcggcatag accatcggcc aggcagcaaa gagtgcgccg
    25261 cccgcggtga tcagccaaac ctggttaccg tcccagtgtg gtgcaatgga gttaatcata
    25321 attcgacgct cggtgtcgtt acgaccgagg aaacgggtga gcatgcccac ccccatgtcg
    25381 aaaccgtcag tgactgcaaa accaatcagc agaacgccaa ccagcagcca ccagataaaa
    25441 cgcaatactt cataatcgat catttgacga ctcctgtctt agcgtgccgg ctgagtagtc
    25501 gtggaagact gctcaaagtg atagcgaccg gttttcaggc tgcttgggcc gaggcgtgca
    25561 aacttgaaca ttaagaacaa ttctgccacc aggaacaggg tatacaggcc gcaaatcagc
    25621 accattgaga agatgagatc gcctgcggtc agtgacgaat tcgccacagc tgtcggcagc
    25681 acttcaccga tagcccacgg ttggcggcca tattcagcca cgaaccagcc cgcttctaca
    25741 gcaatccacg gcagcggaat accgtacagc gcggcgcgca gaagccattt tttctcgcca
    25801 atgcggttgc ggatgacact ccagaaagag agcgcgatga ttgccagcag caggaagcca
    25861 cacgccacca tgatacggaa cgcaaagtac agcggcgcta cacgcgggat ggagtctttg
    25921 gttgcctgtt gaatctgcgc ttcagtcgca tcagccacgt ttggcgtata gcgtttcagc
    25981 agcagaccgt aaccgaggtc tttcttcatg ctattgaact ggtcacgaac cgtctggtcg
    26041 gtagaaccag aacgcagttg ttcgagcaga gagtacgcct tcatcccgtt acgaatgcgt
    26101 tcttcatgct gcaccatcag ctctttcagg ccgataaccg gggtatccac ggaacgcgtt
    26161 gcaatgatgc ccagtgcgta agggatctga atcgcaaatt tgttcgtctc ctcttcctga
    26221 tcaggaatgc cgaacaaagt aaaggcagca ggcgcaggtt gcgtttccca ctcggcttca
    26281 atagcagcca gtttggtttt ctgcacgtcg cccatttcgt agccggattc atcacccaga
    26341 acaataacag acagaacagc agccataccg aagctggcag cgatagcaaa ggagcgttta
    26401 gcgaaggcga agtcacgacc tttcagcata taccatgcgc tgataccgag gatgaacatc
    26461 gcgccagtca cataaccaga cgctacagtg tgaacgaatt tcacctgagc aaccgggtta
    26521 agcaccagct cggagaagct caccatctcc atacgcatag tttcaaagtt gaaatcggac
    26581 gcgattgggt tttgcatcca gccgttcgca accagaatcc acagtgcgga caggtttgaa
    26641 ccgagcgcca ccagccaggt gacacacata tgctgaactt tacccagacg atcccaaccg
    26701 aagaagaaca gacctacaaa ggtggattcg aggaagaagg ccatcagacc ttcgattgcc
    26761 agcggcgcac cgaagatatc ccctacatag tgggaatagt aagaccagtt agtcccgaac
    26821 tggaactcca tggtcagacc ggtagccaca cccagagcga agttgatacc aaacaacttg
    26881 ccccagaact tggtcatatc tttataaatc tgtttgccgg agaggacgta gaccgtttcc
    26941 ataatggcca ggaggaacgc cataccgagc gtcagtggca caaaaaggaa gtggtacatc
    27001 gcggtcaagg caaactgtaa gcgcgacagt tcgactatat ctaacatcat gactccttgc
    27061 tcatcgcatg aagactccga gagtgaaccc cgttagaaag ggtcacacgc atgccccaat
    27121 agaaatttgt ttgaacactt tcatcatacc atgtgaatgc acacaaaaac ggcgtaaaga
    27181 aaggttgcaa acatgttaat aaaaactcaa attgatccca cgtatatatt acgccgcaaa
    27241 atccttacaa taaacaggtt tttgttgaag caaatttgca tttttcgacg gtgatcaatt
    27301 tatagctaaa ttgccgcctt tcagccaatt tgatcgagaa caatttatct ctttttgatg
    27361 cccatttcca agacttatac attgataaat atcaatttct acaaattatg ttaatcatgt
    27421 ttcaacaagg taactttagt gatatttatg tttaaaatgt gtgaattata agactgaaat
    27481 tagtttcggt tggcaattaa agataaaaag agacgaagca aaaacagcac ggttgacctt
    27541 aattcttttt ttggtcactg gctaatacga cgcagagttt caggattatt aataaaggca
    27601 taaatctccc tcccgccgat attaacattt aattaactta taattatatt ataattaact
    27661 cacccgtggt tattattttg ttaaaaataa agccacatat acgtagcttg agagtgaaga
    27721 taaacacaaa attacctgat gcgctacgct tgccagtgtc tacatcatct ctgcaatata
    27781 ttgaatttgc ctgcttttgt agaccgaata aagcgttcat gccgtatccg gcatgaacaa
    27841 agcgcccttt gtcaacaatc tggccgcgca tatgtggctt cggtttgatt gctattcagg
    27901 caagccggta actgaacgtc cgtgactggc cgggtaaaaa aggccccgat agatttgaac
    27961 cttgattttc ctcggtggta atgtgttcat ccatcatcgt ttctgagcaa gaaatcacct
    28021 cgcgactgaa agcaacagtc gcatcacagg ttgctgattc agccggatta aacagccgta
    28081 aaatcacttc ttgtcggtct tcagctttct taagtgcgct tatcaggcat cccactgggg
    28141 gcattttcaa caaactataa ctttccggca cgttgaatcc ggctttgttg agcttcatcg
    28201 catcccatgg gattttgttg taacactgta ctggagtcag ccatgctcgc gcctgctgag
    28261 ctacaccagc ggcggttggc gtaccggtat aactcaataa acttaggcga caagaaagca
    28321 gaccacgtag ttgtgagtcc gggactggca ttttaattcc cgaaggccgc ctaggcctta
    28381 aaagcagatc ttctttcccc agtaagccca cgccgcgcag caacgtaatg gcaaaggttt
    28441 tcttctcttc accgatgact tcaaattcac gtagcccttc gctaaagaca gccatgccgt
    28501 tacgcccttc ctgtaaggct gcatagttga gcatattcca taccggaacc ggcgcttctt
    28561 tccagctttc ttgctgccag ttgttcattg cactgtcgtt cacggggcgc gttagcgaac
    28621 caaactgggt atctgccaga acactatcgg tgttaaaaga tgtagggatc aggacgcgaa
    28681 ggcgatgatc gtcagcctgg ttatcaaggt tgatatccac atcaatgcgc ctgttgttat
    28741 gactaagagt gacaaccaac tcgacatcta ctctgccagt ggattgtctt gcgctgcgtt
    28801 gttgcagatt acgcggcacc gccatttcat agcggataac agccctgctc tgccaggcct
    28861 catgaataat ctcgcattgc ggtttcgcat tcgccgaagt aattacccac tcttcttttg
    28921 cgggtgaata gtcatattca tcaccatcat cagagctttc ttcaatttgc aacacccggt
    28981 catagcgcac accgctatct ttatctacca gttgtagcgt tccatcctca ttgagcgcaa
    29041 tttgccagaa agcattttcc agtatccctt cagcgtcact ttttgccgca atcacgttgc
    29101 caggctgatt cgcttcgata taaagcgtgc gatagcccat agaagggaca atctggttga
    29161 tctgtatatc aaactccata aagggatcgt aattaccgta atgaactatt tgccgatcga
    29221 ttaggcctgg atcgatctca cgggcatggc gaataaaata cggtacaggc tgaccgcgat
    29281 cgtctcgtag attaaactgg ctggcgcgca gccgcacagt ggtgttgatc acttcttccc
    29341 gcggccaggg catcaggtta aacaggacga gtttgtcggc gtcgctctgc ggcatgttgt
    29401 cggcgatttt acgcatatag aaacgcagca gattatccgc catgtcttca gccagttcga
    29461 agcgggcgac gatttcgcga tgaactttgt cacgacagca gcagccgatg ctgtcgtggg
    29521 catgattttt taagatctct ttccacattt tctccagcaa gccgtggtgg tatccaaaac
    29581 ccaacgtcct ggccagcgtt gccagcggtt ccagcagatt aacaatctta ttttcaatac
    29641 gtgcgtgggc aactttgata tccatgcgcg tggaaccgat ggtgcgatgc acgcgcatat
    29701 atttgccatc aataaattcc cctttcaggg ttgccagatt atctcgctgc gcttcgatct
    29761 tctcaaatac ctcttcaaag cggctcatca caaactttcg ttgaggataa atttcgcgca
    29821 gtttctccat cacttcgaag atattttgct gcaatggcat ctgatcatgc ccattcggca
    29881 gcaaaatctc tttggttaca gacgcttttt ccagcacgtc aaaataactg tcgaggcgtt
    29941 tgcgtaatcc gttttcgtcc gcaggtaagt acttaccgat ggcataaccc aacggcatca
    30001 cctgcgccgt cacttcgcta ccgtcactgc tttgccacaa aaactcggtt ttatccgtac
    30061 cgtggcgctc agaacatcca cgccagaaca tggtgcaggt aatgccaaat ccattgtaga
    30121 tatgcggcag ttgcccggac atgccaaagg aatcgggtaa ataacctatt ttcatcggct
    30181 cgccaaacgc gaggcagtca cgcattccgt acatcagatt acggacgatg gattccgcag
    30241 aaacaatcgt ggtatcggtc tgggtatacc aggggccgat aatcaacttg ccggcttcta
    30301 ccagtttctt caagcggtct ttgttttccg gtttcaccgc gaaataatct tcgaggatcg
    30361 ccgtttgccc gtcgagtacg taatatttgt attcgttgtc ctgttccagt cggcacagga
    30421 tctcttccat attattgacc agcagaatgc gtgactcttc ggtggtgaaa taccactccc
    30481 gatcccagtg catatgcggg gtgatgtgaa cgcgagatac cgctttcatc gtcgtttcct
    30541 ttttgtttat ggcattacgc catcagtcag atagttgcca tgcttaaccg cgtgacgccg
    30601 ccagatcagg agaattgcag tcgagattgc agcccccacc aatgccgcac caaaccagcc
    30661 aattgccgcc ataacaccgc ccgcgccatt atcatgaagt aaaaagagcg agaaaatgcc
    30721 agcaccgggt gtcgaaaggc cgatattcat cgcaccgaca atagcgcccg ttaccataga
    30781 gcccagcaca aacgaaccaa taacccgcag cggatcttca atcgccatcg ggatcgcccc
    30841 ttcggtaata cctgccagcc ctaacagcca ggtggatttc ccggtctcaa tttcaaactc
    30901 tttaaacagg cgcggtgcga gcatcgtgga agcggttacg gtaaatgccg aaaccatttt
    30961 gacggaggcg aaaatggcat acgggccgta aacgccgttc gccattgcgc ccaggcagaa
    31021 tgcataagcg gctttattca ctggccctcc aaggtcaaag gaacacataa aaccgagaat
    31081 ggcacccagc aacagcgcgt tacttcctga cagaccgttc agccaggcgg taagcgagtt
    31141 attgatccag gcgacaggtt cccccaccac aaacagcatc agactgcccg ctcccaatgt
    31201 accgagcacc gggtagagat aaaaagtcag gaagccattg aatttactgc taagacgcaa
    31261 gtgatttttc atccagcgta tcaagtaacc ggctatcaat ccgccaacga ccgcgccgag
    31321 aaacccggag ccgatcatgt tggcggcaag tccagccgca aagcctggcg ctaacgccgg
    31381 tttatctgcc agcgaatagg cggtataggc tgccagcacc ggtaccatca aaattccgag
    31441 cagcccgccg cccagtttgc ggtacatcca cagccaggag ttttcttcat taaacagatc
    31501 ttgtagcccg aagatttgcg acagtaatac cgcgaccgcc agcaccgtcc cccccgcgac
    31561 aatcaacggt acggcaaaag agatcccgct caacagtgcc tgtttcagct ccgttttgac
    31621 gcttttcacc ggttgcgtat cttgctgtaa ggtacgcgtc tcatcgccac gctcgagggt
    31681 aagcgattgt tggatcaacg cttctgcatg gcgaatcggc tcggcaacag gcacagaaag
    31741 cgcaggaatg ccgttaaaac gctcactctc cttgatggcg acttcagccg caaaaataca
    31801 ggcagttgca ctattgagtt gatccgccgt taaacgccct tcaataccgt tagcaccttg
    31861 cttttcaacg tagacattca cgccgagttt gcgtccggct ttttccagat actccgcagc
    31921 catgtaggtg tgagcaatac ccgccgggca ggccgttacg caaacgatag ttggcgcgtt
    31981 ggaaaaagag gcagaaggtt gcgtgcctcc cttgtcatcc agcgccgaga gcaattcatc
    32041 aggcgtcgtc gccgactgta tacgcgcccg aatctcatca tccgccaggc gcgtggtcag
    32101 cgctgtcagc agttgcatgt gcgtagtacc cgcttcattg aggggaatcg ccagcaacac
    32161 cactaaatca accgcttccg ggccatccac gccttcccac tgaagcggct cgctgagtgt
    32221 ggcgaccgca aacgccgctt cttttaccgc agcagttttg ccatgcggca cagccaaccc
    32281 ttcacttaag gcggtcgggc caaggctttc acggcgatac acttctttca gaaattgctc
    32341 agtactggaa attttcccca gagcagcaag acgttgagtc aacgcgtgaa tggcctcttc
    32401 acggctggta aagcgcgcat tcagacacaa cgcatcgcgg tgggtcagag tcgtcaggtt
    32461 catcatgatc ctcacttgat cgtttttata gtcgcgccag tgtgcccgac aaaacaatac
    32521 cattgcgtaa tcaatttcac ttgattgaaa ttatttgcat tttatttgta ttgtttacag
    32581 gatgagttta tcgggcataa tggctaaatt cgtattaatg agatgctaaa taatgggaca
    32641 caaaccctta taccgacaga ttgctgatcg cattcgtgag caaattgcgc gtggcgagct
    32701 taaacctggc gatgcgttac ctaccgaatc ggcattacaa acagagtttg gcgtcagccg
    32761 ggttacggtg cgtcaggctt tgcgccagtt agtcgagcaa cagatcctcg aaagcattca
    32821 gggcagcggg acttacgtca aagaagagcg ggtcaattac gatatttttc agttaaccag
    32881 ttttgatgaa aaactgtcgg accgtcacgt cgatacacac agtgaagttc tgatatttga
    32941 agtgattccg gctgacgatt ttcttcagca acagctacaa atcaccgtgc aggatcgtgt
    33001 ttggcatgtg aagcgcgtgc gctatcgcaa gcaaaagcca atggcgctgg aagaaacctg
    33061 gatgccgctt gcgttgttcc cggatctcac ctggcaggtc atggaaaatt cgaaatatca
    33121 ctttattgaa gaagtgaaga agatggttat cgatcgtagc gaacaggaaa tcattcctct
    33181 gatgccaacc gaagagatga gccgtctgct taatatcagc cagacaaagc cgattcttga
    33241 aaaagtatcg cgtggatatt tagttgatgg tcgggtgttt gaatatagcc gcaacgcttt
    33301 taataccgat gactataaat ttaccctgat agcgcagcga aaatcatcgc gataagcaca
    33361 aaaaggccat catacgatgg ccaaccatgt cgaaaccgac agcccttcgc cattcctgta
    33421 gagggaagcg gcgagggcta tttcttatta cagatattta tttcagaaca gttttcagtg
    33481 cttcaccgat atccgccagg ctgcgaacgg ttttcacgcc tgcggcttcc agagcagcga
    33541 atttctcatc cgcagtccct ttcccaccgg caatgatcgc acccgcgtgg cccatacgtt
    33601 tgcctttcgg cgcagtcaca ccagcgatgt aacccacaac tggcttggta acatgctctt
    33661 tgatgtacgc tgccgcttct tcttcagcgc taccgccgat ctcaccgatc atcacgatcg
    33721 cttcggtctg cggatctttt tcgaacattt cgagaatgtc gataaagtta gagccaggga
    33781 tcgggtcacc gccgataccg acacaggtcg actgaccgaa accgtaatcc gtggtctgtt
    33841 taaccgcttc ataggtcagt gtaccggaac gggaaacgat acccacttta cccggtttgt
    33901 gaatgtgacc cggctggata ccaattttgc attcacccgg ggtgataacg cctgggcagt
    33961 tcgggccgat catacgaacg cctgcttcgt ccagtttcac tttcacggtc agcatatcca
    34021 gcgtcgggat gccttcagtg atggtgataa tcagtttgat gcctgcatcg atagcttcca
    34081 gaatggagtc tttgcagaac ggtgctggta cgtaaataac agacgcggaa gcgccagtag
    34141 cagcaacagc ttcacgcacg gtgttgaaca ccggcaggcc gaggtgggta gtgccgcctt
    34201 tacctggagt tacgccacca accattttag tgccgtatgc aatggcctgt tctgagtgga
    34261 aagtcccctg gctaccggta aagccctggc agataacctt ggtgttttta tcaattaaaa
    34321 tggacattat ttcccctcca ctgcggcaac aacctgctga gctgcatccg tcagaccttt
    34381 tgctgcaata atattcaggc cgctgtcagc cagtttcttc gcgccgagtt cggcgttgtt
    34441 accttccaga cgtaccacga ccggtacgtt aacacccact tccgctaccg cgccgatgat
    34501 accgtcagcg atcaggtcgc aacgaacgat accgccgaag atgttaacca gaacggcttt
    34561 cactttgtcg tcagagagga tgattttgaa cgcttcggtt acacgttctt tggttgcgcc
    34621 gccgccaacg tccaggaagt tagccggttc gccgccgtgc agtttaacta tgtccatcgt
    34681 acccatcgcc agacctgcgc cgttaaccat acaaccgatg ttaccgtcca gcgcaacgta
    34741 gttcagttcc cactgtgcag cctgtgcttc acgcggatct tcctgcgact ggtcacgcat
    34801 ttcgcgcaga tcaggctggc ggaacagggc gttgccatca gcgcccagtt tgccgtcgag
    34861 gcaaatcaga tcgccctgtt tggtgatgac cagcgggttg atttcgatca gcgccaggtc
    34921 gcgctccagg aaaatggtcg ccaggcccat gaagattttg gtgaactgct gaaccagttt
    34981 accttccaga cccagtttga acgccagctc gcgtccctga tacggcatcg ggccagtcag
    35041 cggatcaagc gcaactttat ggatcaggtg cggagtttct tccgccactt tttcgatttc
    35101 cacgccgcct tcggtggagg ccataaagac cacgcgacgg gaactacggt ctacaacggc
    35161 accgagatac agctctttag cgatatcggt cgccgcttca accagaatct ggttaaccgg
    35221 ttggccattg gcatctgttt gatacgttac cagacgcttg cccagccagt tttctgcaaa
    35281 agcacggatg tcttctttgc tgtttacaac tttcacaccg cccgctttac cgcggccacc
    35341 agcgtgaacc tgacatttca ctacccacgg accggcaccg atttttgaag cggcttcttc
    35401 tgcttcgcgc ggagtagtac aggcataacc caccggtgct ggtaagccat agcgggcaaa
    35461 cagttgtttt gcctgatatt catgtaagtt catgtgttct gtccatcctt cagtaatcgt
    35521 tatcttttaa accgtagacc gacaccctat ccacgtcggt ctacagtgca ggtgaagctt
    35581 aaactactac acgtccagca gcagacgcgt cggctcttcc agtaactctt tgatcgttac
    35641 caggaagccc acggattcgc gaccatcgat cagacggtga tcgtaggaca gcgccaggta
    35701 catcatcggc aggatctcaa cctgaccatt caccgccatc ggacgatctt tgatagcgtg
    35761 catacccaga attgcgctct gcggcgggtt gatgatcggc gtagacatca gggaaccgaa
    35821 cacaccaccg ttggtgatgg tgaagttacc accggtcaga tcttcaaccg tcagcttgcc
    35881 atcacggcct ttgactgcca gctctttgat tttcttctcg atgtctgcca tgccgagggt
    35941 atcgacatca cgcagaaccg gcgtcaccag gccgcgcggc gtagaaaccg ccatgctgac
    36001 gtcgaaatag ttgtggtaaa ccacgtcatc gccgtcgata gacgcgttca cttccgggta
    36061 acgtttcagg gcttcaacca ccgctttcac gtagaaggac ataaagccca gacggatgcc
    36121 gtggcgtttt tcaaacgctt caccgtactg cttacgcaga tccataatcg gcttcatgtt
    36181 gacttcgttg aacgtggtca gcatggcggt ggagtttttc gcttccagca gacgctctgc
    36241 cacacgctta cgcaggcgag tcatcgggac acgtttttca ctacgtgcag ccagagccgg
    36301 ttgcgccgcc ggagcagccg ctgccggagc agactctttc gccggggctt tcgccagatg
    36361 tttttccaca tcttcacgag tcagacgacc acccacaccg gtgcctttaa tggcgctggc
    36421 gtcgagattg tgttcagcca gcaggcgacg gatcgccggg cttaacgcat cgttgttttg
    36481 ctcttccaga gacgcctgct ggcgttgcgc cggagtggac gctttctctt cagatttggc
    36541 gctggtttct ttaccggcgc tgttgccttc acgcaggcga ccaaggatct gacgagacgt
    36601 taccgttgta ccttcatctt ccagaaccgc atccagaatg ccgtctgctg atgccggtac
    36661 ttccagtacc actttgtcag tttcgatttc taccagcact tcatcacgta cgactgcgtc
    36721 gccgggtttt ttatgccagg ttgcgacggt ggcatcggct acggattcag gcaggtcagg
    36781 gaccagaata tctacgctac tcattgtgta tcctttattt attcgacgtt cagcgcgtca
    36841 ttaaccagat cttgttgctg tttctggtga acggacatat accctaccgc cggagaggcg
    36901 gaggccgggc ggcctgcata acgcagagaa gccccaaacg gaatcacttc acggaaatga
    36961 tgctggctgc agtaccatgc gccctggttg agcggctctt cctggcacca gacaaaatcc
    37021 ttgacgtgag caaactgctg caacacttcc tgcatcgctt tatgcgggaa cgggtagagt
    37081 tgctcgatac gcacaatggc gacatcgtgt tgattgttct tacgacgctg ttccagcagg
    37141 tcgtaataaa ccttaccaga acacatcact acgcgcttca cgcccttcgg atcaagctcg
    37201 tcgatttcac cgatggctgg caggaaggtg ccgttcgcca gttcttcgag gctggaaacc
    37261 gccagcggat gacgcagcag ggatttcggc gacatcacga ccagcggacg acgcatcccg
    37321 cgcagcgcct gacgacgcag catgtggtaa acctgtgccg gggtagacgg tacgcaaacc
    37381 tgcatgtttt gctcagcaca aagttgcaga taacgttcca gacgcgcgga ggagtgctcc
    37441 ggcccctgcc cttcgtaacc gtgcggcagc aacatcacca gaccacacat ccggccccat
    37501 ttctgctcgc cagaggagat gaactggtcg ataaccacct gcgcaccgtt ggcgaagtca
    37561 ccgaactgcg cttcccagat ggtcagggtg cgtggttctg cggtggcata accatattca
    37621 aacgccagca cggcttcttc tgacagtacg gagtcccaga cacggaacgc gccctgaccg
    37681 ttatggatgt gttgcagcgg cgtgtaagtg gaaccgttag actggttgtg gatcaccgcg
    37741 tggcggtgga agaaggtacc gcgaccggag tcttcacccg acaggcgaac cggaatgcct
    37801 tcatcaacca gcgtggcgta agcgaggttt tccgcaccgc cccagtcgaa cagtttctca
    37861 ccggctgcca tcgcctggcg atcgccataa atcttggcaa cgcgagactg catttcaact
    37921 gcttctggta ccgtactgat acgtttagcc agttcctgca ggcgcttcat ctcaactttg
    37981 ttcgggtact cttcgtccca ttcgtggttg aggtacggcg accaggtgaa agagtgcatg
    38041 ttcatcggac gccactctgc cactacgcaa tcgccagcat ccagcgcatc gcggtacagg
    38101 ttaaccatct cggtggcatc ttccagcgtc gccacttttt cctgctccag cttgtcagcg
    38161 tagattttgc gcggtgtcgg atgttttttg attttctgat acatcagtgg ctgggttgcg
    38221 ctcggctcgt cggcttcgtt gtggccgtga cggcggtagc ataccaggtc gatgaagaca
    38281 tcacgtttaa aggtgttacg gaaatcgagc gccagacggg tcacaaaggc aacggcttcc
    38341 ggatcatccg cgttaacgtg gaaaatcggt gcctgaacca tcttaccgat atcagtacag
    38401 tacggtgtag aacgggcatc cagcgggtta gaggtggtga aaccaacctg gttgttgata
    38461 acgatacgta ccgtaccgcc aacttcataa ccacgcgctt tcgacatgtt cagagtttcc
    38521 tgaaccacgc cctgcccggt cactgcggcg tcaccgtgga tggtgattgg cagcactttg
    38581 ttgctgttcg gctcatcaag tctgtccaga cgggcacgaa cagagccgat aacgaccggg
    38641 cttacaatct caaggtgaga cgggttaaac gccagcgcca ggtgcaccag gccgccatcg
    38701 gtctggaagt cagacgagaa gcccatgtgg tatttcacgt cgcctgtgcc gaggtgttct
    38761 ttatgtttac cggcgaactc gtcgaacaag tcttgcggtt ttttacccag cacgttcacc
    38821 agcacgttca gacgaccacg gtgcgccatc ccgagaacca cttcgcgggt gccgctgttg
    38881 ccagcgtggc ggatcatctc tttaagcatc gggattaacg cgtcaccgcc ttccagcgag
    38941 aagcgttttg cgccagggaa ttttgcgccg aggtaacgct caaggccttc agcggcggtc
    39001 agttcgctta agaagcgttt tttctcttcg ctattgaaag tcgcgcgacc agactcgata
    39061 cgctgttgga tccagcgttt ttcttcggtg ctggtgatgt gcatatactc ggcaccaatc
    39121 gggccgcagt aggtttgctt gagggcggaa ataagctcgc cgagtttcat ggtttctttg
    39181 ccgctggcaa atgaaccgac gttgaaggtt tcctggaagt ctgcttcggt cagatcgtgg
    39241 aaagacggat ccagatcggc cactttatct tgctgccaca gtcccagcgg atctagattc
    39301 gcatgctggt gaccacggaa gcggtatgcg ttaatgagct gcaggacttt aacctgcttc
    39361 acattggtgt cagggtcgga gatcgttgaa gagtaacgtg aagcgtcttt cgccaggcgg
    39421 cggaaatatt cacgcgtttg agagtggaat tgatccggtt tgactcccgt accaggtaac
    39481 tgctggaacg tcgaacgcca gttagcgtca accgagtcag gatcggttaa gaagtcttca
    39541 tagagctgtt ctatccagct ctggtttgcg ccagagaggt aagaagagtc caaccaggct
    39601 ttcaaagcgc tgttctgcat cgtgatccct taagcatctt ttttatgctt acttcgccgt
    39661 ggatactacc acgcacagtg atgtgcgtgc cggggttcac ttgcgagctc ttgtctacgt
    39721 agtggctcgc gaaggaacct ttaaaaactg tcttagtgtc aggggcagtt tttaaagatt
    39781 tcctgcattt ctttgttacg cctgatgcgc tgcgcttatc aggcctgcgg gtaatgactt
    39841 gtaggccgga taaggcgttc acgccgcatc cggcactggt tgcctgatgc aacgcttgcg
    39901 cgtcttatca ggcctacagt ttacgcatta cgttgcaaca acatcgactt gatatggccg
    39961 atggcgcgcg tcgggttcag ccccttcgga catacactga cgcagttcat gatgctgtga
    40021 cagcggaata cgctgaatgc atcgctcaaa ccgtcgaggc ggctgtcagt ctcggtatca
    40081 cggctatcga tcaggaaacg atatgccgct aacaagcctg ccgggccgat aaacttatcg
    40141 ggattccacc agaaagacgg acaagaggtt gaacaacatg cgcagagaat acattcatac
    40201 agcccgtcga gtttttcgcg ctgctctggc atctgtaaat gctcgcgagc tggcggattt
    40261 tgtccattat tcaacaggta aggcttaatt ttctcatatt gcgcatagaa ttgtcccatg
    40321 tctaccacca aatcgcggat caccggtaaa cctggcagcg ggcgaatcac aatcttcttg
    40381 cccggctggt tgagtgccga aatcggggta atacaggcca gaccattctt gccgttcatg
    40441 ttcagaccgt cggaaccgca cacaccttca cggcaggagc ggcggaacga caggctggga
    40501 tctttctctt tcagctggat aagcgcatcc agcagcatca tgtcgcgacc ttcttccgct
    40561 tccagggtgt aatcctgcat acgcggagca tcatcaacat ccgggttata gcgataaatt
    40621 gaaaactcga gtctcatttt cctgtctccg cattagtaag tacgaatctt cggcgggaat
    40681 gccgggcgca gtttcggttc catgttgacg cttcggcgcg tcatggattc cgactcaggc
    40741 agatacaggg agtggcacag ccagttttca tcatcacgat ccgggaagtc gaagcggcta
    40801 tgtgcgccac ggctttcggt acggaagttg gcagaaacag ccgttgcata cgccgtttcc
    40861 atcaggttat ccagttccag gcactcaacg cgctgggtgt tgaactcgct ggaagtgtca
    40921 tccagacggg catttttcag acgctcgcgg atcactttca actgctcaag ccctttcgcc
    40981 atcgcatcac cttcacggaa gaccgagaag ttatgctgca tacattcttg cagcgcttta
    41041 cggatcgcca ccggatcttc accgttacga ttattgttcc accggttcag gcgatccaga
    41101 gacgcttcaa catcagactc gctggcatcg tgcagtgcgc cctgctcggc gatagactct
    41161 tgcagatgca gacctgccgc gcgaccaaag accaccaggt ccagcagcga gttgccgccc
    41221 agacggttag cgccgtgtac cgatacacaa gcgatttcac caacggcaaa cagtcccgga
    41281 acaaccacat cttcgccttt ctcattcaca gtcagcgcct gaccggtaac tttggtcgga
    41341 ataccgccca tcatgtagtg acaggttggg ataaccggaa tcggctcttt caccggatcg
    41401 acgtgagcga aggtacggga aagctccagg atacccggca gacgggattc gagaacttct
    41461 ttacccaggt gatcgagttt cagtttcgcg tgtggccccc acggaccatc acagccgcga
    41521 ccttcacgga tttcaatcat gatggaacgc gccaccacat cacggcccgc caggtctttg
    41581 gcgttcggcg cataacgctc cataaagcgt tcgccatgtt tgttcagcag ataaccgcct
    41641 tcaccacggc aaccttcggt gaccagtacg cccgcaccgg caatgccggt cgggtggaat
    41701 tgccacattt ccatatcctg caccggtacg cctgcgcgga tagccatgcc gacaccgtcg
    41761 ccagtgttaa tgtgggcgtt agtggtggac tgataaatac gccctgctcc gccagtcgcc
    41821 agcacggtag cgcgggcttt gaaataaacc acttcaccgg tttcgatgca cagtgcggta
    41881 caacccacca ccgcgccatc ctggtttttc accagatcca gcgcatacca ctcggagaaa
    41941 atggtggtgt ggtttttcag gttctgctga taaagcgtgt gcaacagtgc gtgaccggta
    42001 cggtcagcag ccgccgcagt gcgtgccgcc tgctcgccgc cgaagttttt cgactgaccg
    42061 ccaaacggac gttgatagat acgaccatca tcgagacgcg agaacggcag gcccatgtgt
    42121 tcgagttcca gaatcgcttc cggcccggtt ttacacatat attcaatcgc gtcctggtca
    42181 ccgatatagt ccgacccttt tacggtgtcg tacatatgcc attcccagtt atcttcatgg
    42241 gtattaccca gcgcaacggt aatgccgcct tgcgcagaaa cggtatggga acgggtcggg
    42301 aagactttag agagcagggc acaggtctgg ccgctctggg aaatttgcag cgccgcgcgc
    42361 atacctgcgc caccggcacc aatcacaact gcatcaaatt ctctgactgg caatttcatc
    42421 acacacccca caccacaacg aatccataaa tcacgtaaac caccagtgca acgacaatca
    42481 ccagttgcag catcaggcgc aaggccagcg gtttaacgta gtcggtcaac acctgccaca
    42541 tgccgatcca ggcatgaatc aagatagaaa acagcgccag cagggtgaac actttggtga
    42601 acgcagaggc gaagaaaccg atccagactt catatgtcag ttcgccactg gtggcgaaaa
    42661 aaccgaccat ataaatgatg tagagcgtca ggacgatagc ggtagcgcga acgaggatga
    42721 aatcatgtac gccattgcgt cctaatgcgg aggcgttgct taccatacga ggactcctgc
    42781 gagaagtgaa agcacgacag taataacaaa ggagattttg gcggagcgtt tacccgcttc
    42841 gaatgtttct tccagatagc caaaatccat catcatgtgg cgaataccta cgacgacgtg
    42901 atacgccaga gcggtaagga tgccccacat gataaatttg acgaagaagc tgcccataat
    42961 cgcggaagct tgctcgaaac cttcagggga agagaggctg gtacccagaa gccactgcag
    43021 gatgcccact gcaacaaagg tgatcacacc ggaaacgcga tggagaatgg acgctatcgc
    43081 cgtgatgggg aaccggatgg tctgtaggtc cagattaaca ggtctttgtt ttttcacatt
    43141 tcttatcatg aataacgccc acatgctgtt cttattattc cctggggact acgggcacag
    43201 aggttaactt tctgttacct ggagacgtcg ggatttcctt cctccggtct gcttgcgggt
    43261 cagacagcgt cctttccata actgcgcgtc atgcaaaaca ctgcttccag atgcgaaaac
    43321 gacacgttac aacgctgggt ggctcgggat tgcagggtgt tccggagacc tggcggcagt
    43381 ataggctgtt cacaaaatca ttacaattaa cctacatata gtttgtcggg ttttatcctg
    43441 aacagtgatc caggtcacga taacaacatt tatttaattt ttaatcatct aatttgacaa
    43501 tcattcaaca aagttgttac aaacattacc aggaaaagca tataatgcgt aaaagttatg
    43561 aagtcggtat ttcacctaag attaacttat gtaacagtgt ggaagtattg accaattcat
    43621 tcgggacagt tattagtggt agacaagttt aataattcgg attgctaagt acttgattcg
    43681 ccatttattc gtcatcaatg gattctttac ctgcaagcgc ccagagctct gtacccaggt
    43741 tttcccctct ttcacagagc ggcgagccaa ataacaaacg ggtaaagcca ggttgatgtg
    43801 cgaaggcaaa tttaagttcc ggcagtctta cgcaataagg cgctaaggag accttaaatg
    43861 gctgatacaa aagcaaaact caacctcaac ggggacacag ctgttgaact ggatgtgctg
    43921 aaaggcacgc tgggtcaaga tgttattgat atccgtactc tcggttcaaa aggtgtgttc
    43981 acctttgacc caggcttcac ttcaaccgca tcctgcgaat ctaaaattac ttttattgat
    44041 ggtgatgaag gtattttgct gcaccgcggt ttcccgatcg atcagctggc gaccgattct
    44101 aactacctag aagtttgtta catcctgctg aatggtgaaa aaccgactca ggaacagtat
    44161 gacgaattta aaactacggt gacccgtcat accatgatcc acgagcagat tacccgtctg
    44221 ttccacgctt tccgtcgcga ctcacatcca atggcagtca tgtgtggtat taccggcgcg
    44281 ctggcggcgt tctatcacga ctcgctggat gttaacaatc ctcgtcatcg tgaaattgcc
    44341 gcgttccgcc tgctgtcgaa aatgccgacc atggccgcga tgtgttacaa gtattccatt
    44401 ggtcagccat ttgtttatcc gcgcaacgat ctctcctatg ccggtaactt cctgaatatg
    44461 atgttctcca cgccgtgcga accgtatgaa gttaatccga ttctggaacg tgctatggac
    44521 cgtattctga tcctgcacgc tgaccatgaa cagaacgcct ctacctccac cgtgcgtacc
    44581 gctggctctt cgggggcgaa cccgtttgcc tgtatcgcag caggtattgc ttcactgtgg
    44641 ggacctgcgc acggtggtgc taacgaagcg gcgctgaaaa tgctggaaga aattagctcc
    44701 gttaaacaca ttccggaatt tgttcgtcgt gcgaaagata aaaatgattc tttccgcctg
    44761 atgggcttcg gtcaccgcgt gtacaaaaat tacgacccgc gcgccaccgt aatgcgtgaa
    44821 acctgccatg aagttctgaa agagctgggc accaaagatg acctgctgga agtggctatg
    44881 gagctggaaa acatcgcgct gaacgacccg tactttatcg agaagaaact gtacccgaac
    44941 gtcgatttct actctggtat catcctgaaa gcgatgggta ttccgtcttc catgttcacc
    45001 gtcattttcg caatggcacg taccgttggc tggatcgccc actggagcga aatgcacagt
    45061 tacggtatga agattgcccg tccgcgtcag ctgtatacag gatatgaaaa acgcgacttt
    45121 aaaagcgata tcaagcgtta atggttgatt gctaagttgt aaatatttta acccgccgtt
    45181 caaatggcgg gttaattttt atatgcctaa acacaaaaaa ttgtaaaaat aaaatccact
    45241 atcggacatt tatatattct aaaaaaaaat acatcggtat aaattataaa caaatagatg
    45301 atttttaatc atctatcttc ctctcagtta catgaataaa ccaccaaata tagcgcaccc
    45361 ctataattcc caatacttta ttcatcaaac atttaattca taccatcaat taattacttt
    45421 tacatttaat aaaattaata atatttttaa gaaaaactaa aaacattcga caatcaaata
    45481 agatattctt gcaccgatat acttttttct tttctgttaa aggactcagt tatgtttaaa
    45541 ggacaaaaaa cattggccgc actggccgta tctctgctgt tcacagcacc tgtttatgcg
    45601 gctgatgaag gatccggtga aattcacttt aaaggtgaag ttattgaagc accgtgtgaa
    45661 atacatcagg atgatattga taaagaggtt gaactcggtc aggtgaccac cagccacatt
    45721 aatcagtcac atcacagcga tgccgttgct gtcgacctgc tcttagtcaa ctgtgatctg
    45781 gaaaactcca gcaacggttc cggtggcaag atttctaaag ttgcagtcac ctttgatagc
    45841 tcagcgaaaa ccaccggcgc agatccgatt ctcaacaaca ccagcacggg tgaagccacc
    45901 ggcgtcggcg tacgtttaat gaataaagat caaagcaata tcgttttagg caccgctact
    45961 ccagatatcg acctggctcc gacctccagc gaacaaacgc tgaatttctt tgcctggatg
    46021 gaacaaattg atcaggctac acctgtaacg ccaggcgcag ttacggcgaa tgcaacctac
    46081 gtgctggatt ataaataagt attactttgg cggcgctatc gccgcctttc ataaaatatt
    46141 tatcaggtat ggacaccgtg aatatttatc gactctcttt tgtatcctgc ctggtcgtgg
    46201 cgatgccttg cgcattggcg gtcgaattca atctgaatgt tctcgataaa tcaatgcgcg
    46261 accgcattga tatttcatta ttaaaggaaa aaggagtcat tgctcccggt gagtattttg
    46321 ttagcgttgc ggtaaataat aaccaaatca gtaacgagca aaagattaac tggcacaaaa
    46381 atgacgataa aaccattccg tgcatcaatg atttactggt cgataaattt ggcttaaaac
    46441 ctgaagtccg tcagtcgtta ccattgataa atcagtgcgt cgattttagc tcccgacctg
    46501 aaatgctctt caatttcgat caagccaatc agcaactgaa tattagtatt ccgcaagcct
    46561 ggctggcgtg gcactcagaa aactggaccc caccctccac atggaaagaa ggtgtcgccg
    46621 gtgtcctgat ggattacaac ttgtttgcca gcagctaccg cccacaggat ggcagcagca
    46681 gcactaatct taacgcctat ggaacagccg gaatcaacac gggggcatgg cgcttacgca
    46741 gtgattatca gttgaatcat actgatagcg atgataacca tgaacagtca ggcgaaatat
    46801 cacgtaccta tctttttcgc ccgttaccgc aattaggttc aaaattaacc ctcggcgaaa
    46861 cggattttag tcccaatatt ttcgacggtt tttcttatac cggcgcggca ctggcaagtg
    46921 acgatcgaat gttgccatgg gaactacgcg gctacgcccc acaaattagc ggtattgcac
    46981 agaccaatgc cacggtgacg atcagtcaat caggccgcgt catttaccag aaaaaagtcc
    47041 caccaggccc atttatcatt gacgacctta atcagtctgt tcagggcaca ctggatgtca
    47101 aagtgacgga agaagatggt cgggtgaaca atttccaggt ttcagcagca tcgacgccct
    47161 tcctgactcg tcagggacag gttcgctata aactggccgc gggtcagcca cggccctcca
    47221 tgtcacatca aactgaaaat gaaacctttt ttagcaatga agtttcctgg gggatgctgt
    47281 caaacacctc gctgtacagc ggcctgctgc tttctggtga tgactaccat tctgccgcaa
    47341 tgggtattgg gcaaaatatg ctgtggcttg gtgcgctgtc gtttgatgtc acgtgggcca
    47401 gtagccattt tgatactcag caggacgagc ggggcttaag ctaccgtttt aattacagca
    47461 aacaagtgga tgccactaac agcacgattt ccctcgccgc ttatcgtttc tccgatcgtc
    47521 attttcacag ctacgccaac tatctggatc acaaatacaa cgacagcgat gcgcaggacg
    47581 aaaaacagac gatcagctta tctgtgggcc aaccgattac cccactaaac ctcaatcttt
    47641 acgccaacct gctacatcaa acctggtgga atgcagacgc ctccacgacc gccaacatca
    47701 cggcaggttt taatgttgat attggtgact ggagagatat ctctatttct acgtcattca
    47761 atacaaccca ttacgaagat aaagatcgcg acaaccagat ttacctgtcg atttcgctcc
    47821 ccttcggtaa cggtggtcgg gttggctatg acatgcaaaa cagtagccac agcaccacac
    47881 accgcatgtc gtggaacgat acgctggatg aacgtaatag ctggggcatg tctgccggac
    47941 tgcaatccga ccgtcctgac aatggagccc aggtgagcgg taactatcag cacctgagtt
    48001 cagcgggtga gtgggatatt tctggtacct atgccgccaa tgattacagt tccgtcagca
    48061 gcagctggag cggttctttc accgcaaccc aatatggtgc agcgtttcat cgccgcagct
    48121 ccaccaatga accacgcctg atggtcagca ccgatggcgt ggcagatatt ccggttcagg
    48181 gcaatctcga ctacaccaac cattttggca ttgcggtggt gccgttgatt tccagttacc
    48241 agccttccac cgtggcggtg aacatgaatg acttacccga cggcgtaaca gttgcagaaa
    48301 acgttatcaa agaaacgtgg attgaaggcg cgataggtta caaatcactg gcttcccgtt
    48361 ccggtaaaga cgttaacgtc atcattcgca acgccagcgg tcagtttcct cccctcggag
    48421 cggatatccg ccaggatgac agcggcatta gcgtggggat ggttggcgag gaaggacatg
    48481 cctggttaag cggagtcgct gaaaatcaaa agtttaccgt ggtctggggt gatagccagc
    48541 attgctcgct ccatcttcct gaacatatgg aagacaccgc aaatcgcctg attttacctt
    48601 gtcattaatt aaggaaatga aaatgacatt tatgaaagga ctaccccttt tgttgttggt
    48661 tgccagtctg tgcagccacg ctgcactaca acccgatcgc actcgtattg tgtttaacgc
    48721 taatgataaa gctaccagtc tgcgggtgga taatcgtagc gataaactcc cctacctcgc
    48781 ttattcctgg ctcgaaaatg aaaaagggga gaaaagcgat gatcttctgg tcgccctgcc
    48841 gccgatacaa cgtctggaac cgaaagcaac aacacaggta cggatagtaa agcaagcctc
    48901 gaccacaaaa ttaccgggcg atcgcgaaac gctatttttc tacaatatgc gtgaaattcc
    48961 tcccgccccg gaaaaaaaca gcgaccatgc cgttctgcaa gtcgccattc agagtcgtat
    49021 caaggtgttc tggcgaccgg ctgcattacg caagaaagca ggagaaaagg tcgaactgca
    49081 gttacaggtc agccagcagg gcaaccaact gacactgaaa aatcctaccg cgtattatct
    49141 gaccattgcc tacctgggac gaaatgaaaa aggcgttctc cccggcttca aaaccgtgat
    49201 ggttgcgcct tttagtacgg tcaacacaaa caccggaaat tatagcggca gccagtttta
    49261 tctcggttat atggatgatt acggcgcgtt acgcatgacg acgctcaact gcagcggaca
    49321 atgccattta caagccgtgg aggcgaagaa atgagtgctg gcaagggagt gttgctcgtc
    49381 atctgtctgt tatttctgtc gttgaagtct gcgcttgccc tgaactgcta ctttggttca
    49441 tccggtggct cggtagaaaa atcagaagcg attcaacctt ttgctgtacc aggcaatgcc
    49501 aaactcggcg ataagatctg ggaatctgac gatattaaaa taagatctgg gaatctgacg
    49561 atattaaaat tcccgtctat tgtgacaaca ataccaacgg caactttgaa agcgaacatg
    49621 tctacgtatg ggtaaacccc tacccggggg tgcaggatcg ttattatcaa ctcggagtaa
    49681 cttacaatgg cgttgattat gacgccaacc agggaaaaag ccgcatcgac actaaccagt
    49741 gtatcgacag taagaacatt gacatttata cccctgatgg gatggcagaa caaaatttgc
    49801 tcgggtgatc ccgctaatat tcacatgtcg cgaacctttc tcgctcgcat gcggttatac
    49861 gtcaaaatac gagaaatgcc gccgcatgat tatcaaagta cgcttagcga ctatatcgtc
    49921 gtgcaatttg acggtgccgg tagcgttaac gaagacccta ctgcccaaaa cctgaaatat
    49981 catattactg gtctggaaaa cattcgcgtg ctggattgca gcgtcaattt ttccatttca
    50041 ccggaaacac agatgattga ttttggtaaa tttaatttgc tggatattcg tcgccacaca
    50101 atgtcgaaaa cattcagtat taagacgaca aaatcacaaa atgaccaatg caccgacgga
    50161 tttaaagtca gttcctcctt ttataccgaa gaaacgttgg ttgaagaaga taaagcattg
    50221 ttgattggta atggtctgaa gctacgtttg ctggatgaaa acgcctcgcc ttataccttc
    50281 aataaatatg ctgaatacgc cgatttcacc agcgacatgt tggtctacga aaaaacctat
    50341 acagctgaac tttcgtccat tgcaggtacc cccatcgagg ctgggccctt cgatacagtg
    50401 gtgcttttta aaattaacta taactgattt gccatgatgc cctttgcagc cggaaagggc
    50461 atctctttca gaaaattgct gattttcccc ttttccttga ttgctgttat tttttcctag
    50521 cccggctttc tttatgctgt atcgatacac caaagagaaa ataatgaggg agcgaaggat
    50581 gccagttttg cagtggggaa tgttatgtgt gctatcactt ctcctttcta ttggtttcct
    50641 cgcggtacat ctcccggcgg cgctattact tgggccgatg atcgccggga tcatcttcag
    50701 tatgcgcgga attactctgc aacttccccg ctccgctttt ctcgccgcgc aggccattct
    50761 tggctgcatg attgcgcaaa acctcactgg ttcgattctc accacgctgg ccgccaactg
    50821 gccgatcgtg cttgctattt tactggtgac gctgctttcc agcgccatcg tgggttggtt
    50881 attggtgcgc tatagctcac tgcctggaaa taccggtgcc tggggttctt ctccaggcgg
    50941 cgcagcggca atggtcgcta tggcgcaaga ttacggcgca gatattcgcc tggtggcgtt
    51001 tatgcaatat ctgcgagtgc tgttcgtcgc gggggccgct gttctggtca cccgcatgat
    51061 gctgggtgat aacgctgaag cggttaacca gcaaatcgtc tggttcccgc cagtgagcat
    51121 taatctcctg cttacgattt tgctcgcagt cgttgccggt acggcgggat gcatgctgcg
    51181 tcttccttcc ggcacgatgc tcatcccgat gctggcgggc gcagtgctcc agtctggtca
    51241 gctcatcacc atcgaactac cggaatggct gctggcgatg gcgtatatgg caattggctg
    51301 gcggattggt cttggtttcg ataaacaaat cttactgcgg gcactacgcc cgctaccgca
    51361 aatcctgctg tcgatttttg ctctgctggc tatttgtgca ggtatggcgt ggggactgac
    51421 ccggtttatg catattgatt ttatgactgc ctacctcgcc accagtcccg gcgggcttga
    51481 taccgtggcg gtcatcgccg cagggagcaa tgccgatatg gcgcttatta tggcgatgca
    51541 aaccctgcgc ctgttcagta ttttgctgac ggggccagcc attgcgcggt ttatttcaac
    51601 ctatgcgccg aagcggtcgg cctagtgctg gcagcctgga caccagtaaa acgggcgcga
    51661 ggacaacgtg gttttctcaa tgatgccgcc acagcgttcg cacagttcgc catcacgatg
    51721 aaaaacctta aagcgaaaca gcgccccatg atgcttattc tcatccacct gcccccgcgt
    51781 agcgtaagaa aaccgaggaa tctccagtaa cgcgtgtgcg agtgcatcca gttgcgccgc
    51841 attgagatct ttcgctttat gatttccagt caacccaacc tgccagagga tctccacccg
    51901 caaataattg ccaagcccag ccagaaacgc ctgatcgagc agtaatcctg caaactgacg
    51961 gttacgaaag cgcggcgaca ataaccgttc ctttaccacc tccggcgtca gattcggatc
    52021 cagcacatcg ggaccaacgc gttgtaaaaa cggatgcgtg gtcagttgtt ccggcctcaa
    52081 catctcaata tccgaggcgc tataaagcag aatggttttg tcagccgttt gcagttttac
    52141 ccgcaatact cgcgtggtct gcggctcttc gccggtatca accacgcgcc agacgccgta
    52201 aagctgatta tggctgtaga gcgttaaacc gttgggaaaa tgagttaaca acgccttacc
    52261 acgcgtttcc acatgggtaa cgtgttgacc gataagttgt gattgataag tttttaactg
    52321 cgggaaggca aaccagacat cagttagtgg tttgcctttg atcgccgcct ccaggttatc
    52381 cgctgcacgg cggatctccg ggccttcagg catggtgata tccttttttc attctttatt
    52441 aagcggggtt atgctgcgac aacaatcccc tttttggcaa atgtagctcg taatcggcgg
    52501 gcgaacgcca gagcgtgctc gccgtcgcca tgcaggcaga ccgtttgcgc cgcgaccgtt
    52561 gcccattcgc cggtgatact tttgactctg ccgtgttgca ccatctccag cgtttgcgcc
    52621 aacgcctgtt cttcgttttc aatcaacgcg cctggctgac ttcgcggcac cagcgagccg
    52681 tcagcctgat aaccgcgatc ggcaaacact tcctcgcgcg ttgtcaggcc atattgcttg
    52741 cctgcacgaa tcagctcgct tcctgccagc ccaacgagaa ccaatgccgg atcgcaagcg
    52801 tataccgctc tggcgatggc gtctgccagt tgtgcttctt tcgccgcctg gttgtacaac
    52861 atgccgtgcg gtttgacatg acgcattacg ccgccttgcg cacgggcaat cgttgccagc
    52921 gcgccaattt gatacagcgt ctgggcgtaa acggtttccg gcggcagctg catggcgctg
    52981 cgaccaaaat tttccctgtc gggaaaactc gggtgagcgc caatcgcgac accatttttt
    53041 attgcttcac gcacgcaagc ctgcatggtt tgcgcatcgc ctgcatgaaa tccacaagca
    53101 atattggcag aggaaaccag cgttaatagc tctgcgtcgc tggcgcagcc ttcgcccaga
    53161 tcggcgttca ggtcaatttt cattgtgcag ccgccacgct aattgttcaa aataacgttg
    53221 ctgatcttgc cgcgctttca gtgcctcttc cagtgaacac tggacaaaat gaatcggctg
    53281 accgagcgga atttgcgcca gatggtacat atcagcctca atgatacagg caatacgtgg
    53341 ataaccaccg gtggtctgtg cgtcgttcat caacacaatc ggctgcccgt tatgcggcac
    53401 ctgcaccacg cccggtaaca aaccgtgaga taacagttcg cgatcggtga tgcgttttaa
    53461 aatttgcccc tgtaaacgat agcccattcg gttactttgc gagctaagct gccagggcga
    53521 acgccagaat gcatcctgcg aggcgcgatc gaactcatga tattccggcc ccggcaaggc
    53581 gcgaatgcgg ttgccccaca gcagctgttt aacgccctgc gcttccataa aatcacgctt
    53641 cgatttgcca atcgggagtc agtcaccatc cttcagtaaa cggccttcca gcccgccaat
    53701 ccccactttg agatcggtgc tgcatgaccc cattaccggc ggaacatcaa taccacccgc
    53761 gaccgccaga taactgcgca tcccgtgctg cgggcgttta agcgttaaac gctggcctgc
    53821 tttcatcggc aatcgccagc cggtccagac ggcgttatca tccaaccgcg cttcgcaacc
    53881 tgcacccgtc agggcaaacc acccatcagt ttcgaactca actgttaact gaccgagcgt
    53941 gatctccagc gcgggggcat tggcgtcatt acccaccagc aggttagcaa tgcgcagcgc
    54001 aggcatatcc agtgcgccac agtggctgat acccgactgg caaaaaccgt gacgaccgcc
    54061 atcctgcaca gtggtgtaca tgcccgcacg aataatcttc agcatattcc ctccttctgc
    54121 ggtacaaatc gcacgctgtc tcccggacgt aataagatgg gttcgtcacg cgccggatca
    54181 aacaggctga gtgaggtatg accaatcaac tgccagccac ccggcgttgc cagcggataa
    54241 acaccagtct gcggcccgcc gatcccgaca gaacctgccg gaacgagcaa gcgcggttca
    54301 gcgcgccgtg gcgtgtgtag ttgttccggc aaactcccga gatacgggaa gcccggttga
    54361 aaacctaaaa accagaccac gtattccacg gaggagtgca attcaacaac ctgtttttcg
    54421 ctcaacccgc aatacgccgc gaccaccgcc aaatccggtc cgcctgcacc accgtaaacc
    54481 accggaattt caataaagcg agactcaggt tccagggcct cgctctcctc ccaccagcgt
    54541 tgcaaacgct ctatggcatc cagcgccagc gactcaggat tacgcaaaat caccgtgata
    54601 ttgttcatgc cgggaatggc ttcaaccaca ttcggcatat ccaccagacg ctgcgccagt
    54661 cgccagatcc gtttctggct agccagcgtc accggcggtt ccagttccag cactaccgcc
    54721 gtttcaccta tcagataaca acgcgctcgt tgcactttcg atccctctga ttattcgtta
    54781 tgcaggatta ggaatatcaa taaaggtcac atcaagatcg gtattttcat tcagccactc
    54841 gctcaatgcg cgaataccac cgcgttcagt ggcatggtga cctgcagcat aaaaatgcaa
    54901 tccctgctcg cgggctgaat gaatggtctg ttcagaaact tcgccagtaa taaaagcatc
    54961 cacgccaaaa cgcgcggcgc tatcgataaa actttgcccg ccgcccgtgc accaggcgac
    55021 gcgctggacc acctcaggtc cggtatcgcc acaccataac ggcttacgtc ccagacgcgc
    55081 ttcaatccag gaggctagtt ccattcccgg cacaggcatg gtcagctctc cccacggcac
    55141 caacggctca atttcgccca tgaccgtgat ccccagtaac gccgccagct gtgcgttatt
    55201 gcccagctca ggatgtgcgt caagcggcaa atgccagcca tacaggttga tatcattcgc
    55261 cagcaacgtt tttaaacggt tacgcttcat tccgcgaatg accggagact ctcctttcca
    55321 gaagtagcca tgatgcacaa tgaccgcatc cgcgcccaga cgcactgcct catcgagcaa
    55381 agcctggctg gcggtgacac cggtaacaat tttttgcacc gtctctttgc cttccacctg
    55441 caaaccattc ggcgcgtaat cactaatcgc cgcgctgttc agtttttcgt taatcagttg
    55501 ttccagttcg gtgtttttca tcttcgcgcc ttccgtattc tgatagctac accgttaaca
    55561 tagcgttaca tcgcctgttc gtcgatagcc aatcttttga catattaaga aacaaacccg
    55621 acgccaaaag acgcaaattg tttatatcat cacgcttcag ctaatgtata tttatgcaat
    55681 cagacagcat attgcataaa taatcagatc tgtatcaaag ttttaacttt aaggaaacac
    55741 aagcacgccg cgcaactatt acgtgacgtt gccgtcaatt gtggaattat tatgaataaa
    55801 cacgcatcac aaccgcgcgc tatttactat gtcgttgcgc tgcaaatctg ggaatatttt
    55861 agcttttacg gcatgcgtgc cctgctgatt ctctatctca ccaatcaact aaaatacaac
    55921 gatactcacg cctacgagtt atttagcgcc tactgttcgc tggtgtatgt cacgccaatc
    55981 ctcggtggct ttttggcgga taaagttctc ggcaatcgca tggcggtgat gctgggggcg
    56041 ttgttgatgg cgatcggtca tgtggtgctg ggtgccagtg agatccatcc gtcattcctc
    56101 tatctgtccc tggcgattat cgtctgcggc tatggcctgt ttaaatctaa cgtaagctgt
    56161 ctgctcggcg agctgtatga gccaaccgat ccgcgtcgtg atggcggttt ctcgctgatg
    56221 tatgcggcgg gtaacgtggg gtctattatc gcacctatcg cctgtggtta cgcccaggaa
    56281 gagtacagct gggcgatggg ctttggcctg gcggcggttg gaatgatcgc gggtctggtc
    56341 attttcttat gtggcaatcg tcatttcact catacccgcg gcgttaacaa aaaagtactg
    56401 cgtgcgacaa actttctcct gccgaactgg ggatggctgc tggttctgct ggtggcaacg
    56461 cctgcactga ttaccgtgct gttctggaaa gagtggtcgg tatacgcctt aattgtcgcg
    56521 actattattg gcctgggtgt actggcaaaa atttatcgca aagcagaaaa ccagaaacag
    56581 cggaaggagc tggggctgat tgtgacgctc accttcttca gtatgttgtt ctgggccttc
    56641 gcacaacagg gcggtagcta gattagcctt tatatcgacc gcttcgttaa ccgcgatatg
    56701 tttggttata ccgttccgac cgcgatgttc cagtcgatta atgccttcgc agttatgctg
    56761 tgcggtgtgt tcctggcgtg ggtggttaaa gaaagcgtcg caggtaatcg taccgtgcgc
    56821 atctggggaa aatttgctct tggccttggc ctgatgagcg ccggattctg cattctgacc
    56881 ttaagcgccc gctggtccgc aatgtatggt cactcttctc tgccactgat ggtgttaggc
    56941 ctcgcggtga tgggctttgc ggaactgttt atcgacccgg ttgccatgtc gcaaattacg
    57001 cgcattgaaa ttccgggcgt gaccggcgta ttaaccggca tctacatgct gctttctggc
    57061 gcgatcgcga actatctggc gggcgtgatt gccgatcaga catcgcaggc ttcgtttgat
    57121 gcttccgggg cgatcaacta ctccatcaat gcatatattg aagtatttga tcaaattacc
    57181 tggggcgcac tggcgtgtgt aggactggta ctgatgattt ggctgtatca ggcgctgaaa
    57241 ttcagaaacc gcgcgctggc gctggagtca taataggcca gcaccgatgc ctgatgcgac
    57301 gcttgacgcg tcttatcagg cctacgtatt tcctgcaatt tattgaattt gcacaaattt
    57361 gtaggctgga ttaggcgttc acgccgcatc cggcatctgg gctcgattgc ctgacgcgtc
    57421 tgttatttcc ccttccgcgc cgcctcatac gctgccaacg tttgtactct cgcctctttg
    57481 tgctcgacta tcggttgcgg ataatccagc ttcacacctg ctttctgcgc ccacttccac
    57541 ggctcatgca ccgctttccc tggtacatcg cgcagctccg gtaaccatcg acgaataaac
    57601 tcgccctcac ggtcaaattt ctcgccctga gttatcggat tgaaaatacg aaaatacggc
    57661 gcagcatcag tacccgtaga agccgcccac tgccagccac cgttattagc tgccaaatca
    57721 ccatcaatca gctgcgacat gaaatatcgc tcgccttcgc gccagtcgat caacaaatct
    57781 ttcaccagaa aactggctgt aatcatccgt agccggttat gcatccagcc agtgctgtta
    57841 agctgacgca tagcggcatc aataatcggg tatcccgttt tgcctttctg ccaggcctgt
    57901 aaatgtgcgg gattgctttg ccactgcaca cgatccgtcc aggcaataaa tggacgatgt
    57961 ttacacaacg aggggtaata cgtcatcaga tgacggtaga actcgcgcca gatcagctca
    58021 ctaagccaga cactaccggc cccaccgtcc agcgcctgcg gctgttccgc caacaagcga
    58081 tgcaaacact ggcgaggcga taacccgccc gttgccaagc tggcggacaa acggctggtg
    58141 ccttccactg ccggaaaatc tcgttgttgc tcatactctc cagcaccgtt ctcgcaaaac
    58201 tggcgtaatt gcgcaatcgc cgctttttct tccaccggga aatgcgcagt atcgaaagac
    58261 tgacgaggat aattcagcgt aatggatggc gcgggcttta tcgatccgct actacgaact
    58321 tttggtgcag cgacgcactc cggcatccct tcccgcagcc gtttcagcca ggcattctta
    58381 aaaggcgtaa agactttgta catctcgtga ttgccggtca tcaccgcccc cggcggcagg
    58441 atcacgctgt catcaaatcc ttcacacacc acgttacgca gcgctctttc aacgtccaca
    58501 tcccgcgctc gctcattcac ctcatactga tagttataaa aaagatgagt aacgctgttt
    58561 tccgcgcaca cctgtttaac tatttcgaca ctggcgacaa agtcatccac ttcacggaac
    58621 aataaaggaa tacctttttc cgccagcgcg atttgtagcc cattcagttg agcattgata
    58681 agttcagcct gacgcggcga catgttatgc gtctcccact tgcgtggtgt agcgatatac
    58741 agcgccagca cgcgtgcaga cgaattgcgg caggcggcag ccagtgcgag attatcgtgc
    58801 agacgtaaat cctggcgaaa ccaggccaga tgggtagtca taaaactcct gaatggcgca
    58861 agttaatcac acgcggccaa agttcgaaat aacgctgccc tgaaagccag gcgtcaggat
    58921 aaagcgccat atagtgtttg atacgcatca gcggcgcaag aagtggttgc tcgccacggc
    58981 gataactgtc gataagcgca gccagctgct ggcgttctgt ggaatcaata tgcgggcgaa
    59041 aataaccctg aacgtgcatc aaaacgttgg tgtgatcgcg cgggttggcg ggatgagaaa
    59101 gcaaaacaat tacgcgttgg cggtactggt tgtaaaaatc atccagattt tgccactgat
    59161 gaatatcagc cacaaacggg ccaagtttgc ggtactcggg ctgagaatgg gccagaaaga
    59221 cgagtttata gcggctgtga taatcgagaa gcgccccgcg cgttaagcca tgttctttca
    59281 gtcgatgtaa ctcatgaagg gcgaagatac ggattagggt gctgtcatca aatcgttgta
    59341 gattcattac tgctcctgaa cggtatacag gagtaagtgt aggcgttggt aaatgatgtt
    59401 tgtgaagtta atccgctagc agggcaagat tacgaaagcg cgctccccgc aagcaactaa
    59461 tcaaggtagt taatcatggc tgaacacgat attacgccag aagtcactat ttccaaaaca
    59521 cagcgccgct ataaagtcgg ctatgtcaga gcccgccacc aggaccgcaa aaccggtata
    59581 gcccggtatt attcacaaca tcccagtctg caccttaagg gcgactggct gaaagaggcc
    59641 ggatttgata ccggacgggg tgtcacggtg aagatttcgg aggggggcct gaccatcatc
    59701 gctgacagtg acgaggtgca ggaactgcgg gatgggatgt ttagtgtgct gaacgaaagt
    59761 taaatattaa aaaagctggg aagatttaaa accttcctag ctttttattc tgtcttatta
    59821 taatggatac tcatcatctt ccgatgaatt tataaattgc tccaagttta catgaagagt
    59881 acttaaccta gtttttaact cattaagtga agcgataatt tcatattttc caacataatc
    59941 atatgatgga tcgtattcat tatctatgtc agaagctaaa aatgatgata ttcgtagttt
    60001 aaatttagct tcatctttat tacattcatt ggcataaggt gaatgcccta tcatagcata
    60061 ttcatctaat ctaaaaggat aagatggttt taattcatcg atcaatgaaa tttcaatcca
    60121 tgttctatat ccaggcatta atggactaat ttctttgagc aaaacctttc cacttaataa
    60181 tgtattgaga ttgttcatta ttatgtctcc cacgggaaaa atatatgtct aacacttcca
    60241 ccatgttgat gaacttgtaa atgtggtaaa tcaccatcag gatttcctgg cccatgacct
    60301 gacactctat atgaaggttt tgttacagaa ggatctttta tatcatccca atgatatgaa
    60361 cctgcttttg ttttattttc agtaagatat tccattaagt tcttagtgct ggggcctgac
    60421 tcacctgtca tatttttata attatgacct aaatatctat ccataaacaa ttgttcagcg
    60481 tcacttctag acctaacagt aactttggtt tcactactat ctaataaacg attcgctttt
    60541 tggttactaa cagcacatgg agttaatcca agtggatcag catattttag aggattaggt
    60601 gcataactgt agagatttaa accgcctctc aaccctatcg ggtcagttac catgaaccgc
    60661 ccgacatccg gatcatagta cctgtgcaga ttgtagtgca gccccgtttc cctgtcgagg
    60721 tactgtccct ggtagcgcag gttctgctcc accccgccgt ggtccttctc ctgtatcgtg
    60781 ttaccccaga cctgataccc cgtctcccac acgattttcc cgtcgctgtc cgtcatctct
    60841 tccggcgcgc cgttcacatc cgtgtgaaaa tacagtatcc ggttcgggcc ctctttgccc
    60901 gccttatcca cccgcgccag cggttcataa ctcccctggt ccctgtagat gtacaggctg
    60961 cctgttccct ccggcctgct ctcctgcgcc agccgcattc cgtcccataa aaattttgtc
    61021 cggttgtacg gcttaccgtc agggctgatg tgctgttttt ccgtccgcct ccccagtgca
    61081 tcgtacacat accggtatgt cccccgcgct gtcgtcactt ccaccatccg gtgttccgca
    61141 tcataacggt aactctgcgt tccgctccgg ccctcctttt ccaccgcacg gccaaattcg
    61201 tcataacggt agtgcacacc acggtaagac atcagccggt tgaacggcac cacgctgttt
    61261 tctgccgtgg ccctttcccc cgcgcgcctc cccagcaggt ttcccgccgc gtccacctcc
    61321 gtttccggct ggtgccacac cgggtgccac tgcgcctgct ccacccgccc cagcgggtcg
    61381 tgcgtctccg tgatatgccc ctgcaggtca tacacatagc gccatttgcc gccctgaggg
    61441 tcggtcatcc ccagcaacag tcgttcttcg tcgctccagc ggaatgtcgt cacctgtccg
    61501 gcttcgtcct ggtacgccgt cacctgattc tgcgcatccc actggtgata acgtgtgcgc
    61561 cccagtccgt ccgtcacgca cagcctgcgc tgcgccaggt cccacgccag ccggtactgt
    61621 tcaccgctgt ccgtgtggtg ccccgtcacc cgccatgccg cgtcatccgg accttcatac
    61681 cagccccact ggtaataata cccttcgccg cccggctggc ggtgcagcgt catgcagtgg
    61741 ttttcgtcgt aagcaaactc acgcagcagg tgccccgtgc tgtcgtacac gccgttcagg
    61801 tggccggcct cgtcatagct gtaacgacgc agcagtaaag gtgcagtctc agggaaatgc
    61861 tgataaatct gtgtcacccg ccggggatgc gccgccagtt cgtaatacag acggatgcac
    61921 ggacgctgct gttcgccgct gatttccaca atgcggcccc ggtcgtcgta gtacagattc
    61981 agacagttgc tgttacggtc gcccagcatt ttcagccgct ggcgctgcgg gtggtgcggg
    62041 tcagcctcaa acagaaaaca ctgcccgtca tcgctgctga taagccagtg cccctgctca
    62101 ccacgcctta ccgccagccc ttcaccgggg ctgtaaaacc cgccccccgg cagtacggct
    62161 tccatatcca agcgtcgccc catgcccgac acaaaggtca tgcagttttc gtctgcgcta
    62221 cccggtgtgc acacgaggct gacttcatac attacactcc agccctgacc aaacattcct
    62281 tccgtacgtt cgtcacgact gctgtacacg cgctgccagt ccagcggaaa gtggcccggc
    62341 agactgaaat cgaagtcatc gtcatcggcc aggtatttgg ccccggtggc ggcattgacc
    62401 ggatgcggaa gggcactggt ggccttctgc gtcagcatgc tcatcccgac actggcagca
    62461 aaacaggcga ttttagaaag cattttgccc gggcgaagaa acatcagcgc cacggttgcc
    62521 accagggtta tctgcgacct gccgcttctg atatccctga ccaccagtaa cggaccgcca
    62581 atgcgcacat ccggtgacac aaacgccccg ttccccggct cattcaccac cctggcctcg
    62641 caggtacagc gtgccccgct gcgtgtcgcc ggctggctgt tgatacgcac atcccgcgcg
    62701 ccttccgcga tatattccgc tgcgggcggc tcgtcatcgc ctttgaacag attactgatg
    62761 ctgccaataa ccgcattcac tccctgaaag gcggtttcca gcataccgcc cggattcagc
    62821 aggttaaggg ccgttcccag ggggtcatcc agaaaggcgg cttttttttg tgcgagaaac
    62881 tgcgtcagtg aggaaggatg tttatggcag tcgattttat cgtcaggggt cggggtggat
    62941 cccggggccg cactggccac agtgggctgc catagttccg acacaaaatg tgtcacgcca
    63001 tccaccattt cagaacgccc ttcttccgta ctgagctggt cgtgcagagc ccgggacatc
    63061 agcacaggcc ctgtcaccgg gttaaccgcc agggctatgg cccccgccat ttccagatat
    63121 tccccggcca catcccggaa cgttctggca tcctcctgac gctgcgcttc ttcctgttct
    63181 gccttcgcct gcgcttcctg cgcctctatg tcctgccgcg atgccgcagt cgccgctgcc
    63241 cgcgcggcag gtttgctgtt gatccatgtg tcaccagagc cggtgaggat gtaaccttca
    63301 atctgaggcg ggaaaagcat atttgcgagg gctgaacagg cttcgctgac cttttctgtc
    63361 acgccggtgt acaccatcgc cacacccgcc acaaaaccgg tcagcacgac ggccgtgcac
    63421 cccatcgtga tccccactga cgtcgccagt aacgaggttg tgccgacaat gagggcatca
    63481 ataacgacat ttgcggccac ctccagcacc gcccccagca catctgccat aaacgacgta
    63541 tgcagcaggg catcaccctg tcttgccgcc cagaatgcct cttccatgtc ccgttccctg
    63601 tatgattaat tcgtcctgac ttcctgccgc atcctgaagc gcagattctt tttaatttca
    63661 gcccagcgcg tcatatctgc cggggtcagc ggtgtggttc tggcgtaggt gaaggccatc
    63721 atgacccgct cacccggcaa tacaaatgcc acctgacact ggtacacctg ctcgttaccc
    63781 cggagaaact ggctctgtat ttccaaaccg cctcgttgag ataatattcc gtcataatgt
    63841 aaatagtccg ttttaataaa ggaattattt attgacaggt tctgtttttt taatcatgaa
    63901 tgtaataccc atcaccgctt tacagcacac tttatctttc cagtcataat cactaataat
    63961 attaagcaat tagcaatatt tacaacgacc agagcggata catagttatg tatttaacaa
    64021 tcccaatata ttcactatgc aaataaaacc actagtaaca gacacgctac cgcctccata
    64081 taaaaatgga caccttataa aattgtatct tgcatagaaa aagctgaaat atcggatgcg
    64141 ttttaattat caactataaa tctataagct ttttattaac aac
//
LOCUS       DAJTUI010000012        60421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTUI010000012 DAJTUI010000000
VERSION     DAJTUI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.012"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..323
                     /locus_tag="OO464_000828"
     CDS             <1..323
                     /locus_tag="OO464_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6226076.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            436..1230
                     /gene="panB"
                     /locus_tag="OO464_000829"
     CDS             436..1230
                     /gene="panB"
                     /locus_tag="OO464_000829"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR6226077.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1242..2093
                     /gene="panC"
                     /locus_tag="OO464_000830"
     CDS             1242..2093
                     /gene="panC"
                     /locus_tag="OO464_000830"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6226078.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2167..3069)
                     /gene="rpnC"
                     /locus_tag="OO464_000831"
     CDS             complement(2167..3069)
                     /gene="rpnC"
                     /locus_tag="OO464_000831"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR6226079.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3343..3723
                     /gene="panD"
                     /locus_tag="OO464_000832"
     CDS             3343..3723
                     /gene="panD"
                     /locus_tag="OO464_000832"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6226080.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3727..4956)
                     /locus_tag="OO464_000833"
     CDS             complement(3727..4956)
                     /locus_tag="OO464_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6226081.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5020..5460)
                     /locus_tag="OO464_000834"
     CDS             complement(5020..5460)
                     /locus_tag="OO464_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6226082.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5565..6335)
                     /locus_tag="OO464_000835"
     CDS             complement(5565..6335)
                     /locus_tag="OO464_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6226083.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6332..7258)
                     /locus_tag="OO464_000836"
     CDS             complement(6332..7258)
                     /locus_tag="OO464_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6226084.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7367..8029
                     /gene="can"
                     /locus_tag="OO464_000837"
     CDS             7367..8029
                     /gene="can"
                     /locus_tag="OO464_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6226085.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8070..8606)
                     /gene="hpt"
                     /locus_tag="OO464_000838"
     CDS             complement(8070..8606)
                     /gene="hpt"
                     /locus_tag="OO464_000838"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6226086.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8812..11202
                     /gene="gcd"
                     /locus_tag="OO464_000839"
     CDS             8812..11202
                     /gene="gcd"
                     /locus_tag="OO464_000839"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6226087.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11249..12799)
                     /gene="cueO"
                     /locus_tag="OO464_000840"
     CDS             complement(11249..12799)
                     /gene="cueO"
                     /locus_tag="OO464_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR6226088.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12965..13312
                     /locus_tag="OO464_000841"
     CDS             12965..13312
                     /locus_tag="OO464_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6226089.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13418..14284
                     /gene="speE"
                     /locus_tag="OO464_000842"
     CDS             13418..14284
                     /gene="speE"
                     /locus_tag="OO464_000842"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6226090.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14300..15094
                     /gene="speD"
                     /locus_tag="OO464_000843"
     CDS             14300..15094
                     /gene="speD"
                     /locus_tag="OO464_000843"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6226091.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15132..15494)
                     /locus_tag="OO464_000844"
     CDS             complement(15132..15494)
                     /locus_tag="OO464_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6226092.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15669..18266)
                     /gene="acnB"
                     /locus_tag="OO464_000845"
     CDS             complement(15669..18266)
                     /gene="acnB"
                     /locus_tag="OO464_000845"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6226093.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVSVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18621..20474
                     /locus_tag="OO464_000846"
                     /pseudo
     CDS             18621..20474
                     /locus_tag="OO464_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20545..21969)
                     /gene="lpdA"
                     /locus_tag="OO464_000847"
     CDS             complement(20545..21969)
                     /gene="lpdA"
                     /locus_tag="OO464_000847"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6226094.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22294..24174)
                     /gene="aceF"
                     /locus_tag="OO464_000848"
     CDS             complement(22294..24174)
                     /gene="aceF"
                     /locus_tag="OO464_000848"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6226095.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24189..26852)
                     /gene="aceE"
                     /locus_tag="OO464_000849"
     CDS             complement(24189..26852)
                     /gene="aceE"
                     /locus_tag="OO464_000849"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6226096.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27013..27777)
                     /gene="pdhR"
                     /locus_tag="OO464_000850"
     CDS             complement(27013..27777)
                     /gene="pdhR"
                     /locus_tag="OO464_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6226097.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28318..29691
                     /gene="aroP"
                     /locus_tag="OO464_000851"
     CDS             28318..29691
                     /gene="aroP"
                     /locus_tag="OO464_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6226098.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29734..30588)
                     /gene="ampE"
                     /locus_tag="OO464_000852"
     CDS             complement(29734..30588)
                     /gene="ampE"
                     /locus_tag="OO464_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6226099.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30585..31136)
                     /gene="ampD"
                     /locus_tag="OO464_000853"
     CDS             complement(30585..31136)
                     /gene="ampD"
                     /locus_tag="OO464_000853"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6226100.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31224..32117
                     /gene="nadC"
                     /locus_tag="OO464_000854"
     CDS             31224..32117
                     /gene="nadC"
                     /locus_tag="OO464_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6226101.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32320..32760
                     /gene="ppdD"
                     /locus_tag="OO464_000855"
     CDS             32320..32760
                     /gene="ppdD"
                     /locus_tag="OO464_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6226102.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32770..33917
                     /gene="tadA"
                     /locus_tag="OO464_000856"
                     /pseudo
     CDS             32770..33917
                     /gene="tadA"
                     /locus_tag="OO464_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33907..35109
                     /gene="hofC"
                     /locus_tag="OO464_000857"
     CDS             33907..35109
                     /gene="hofC"
                     /locus_tag="OO464_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR6226103.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35144..36187)
                     /gene="guaC"
                     /locus_tag="OO464_000858"
     CDS             complement(35144..36187)
                     /gene="guaC"
                     /locus_tag="OO464_000858"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6226104.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36349..36387)
                     /locus_tag="OO464_000859"
     CDS             complement(36349..36387)
                     /locus_tag="OO464_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226105.1"
                     /translation="MIIKTLKMSARK"
     gene            36412..37032
                     /gene="coaE"
                     /locus_tag="OO464_000860"
     CDS             36412..37032
                     /gene="coaE"
                     /locus_tag="OO464_000860"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6226106.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37032..37775
                     /gene="zapD"
                     /locus_tag="OO464_000861"
     CDS             37032..37775
                     /gene="zapD"
                     /locus_tag="OO464_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6226107.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37785..37982
                     /gene="yacG"
                     /locus_tag="OO464_000862"
     CDS             37785..37982
                     /gene="yacG"
                     /locus_tag="OO464_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6226108.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38082..38471)
                     /gene="mutT"
                     /locus_tag="OO464_000863"
     CDS             complement(38082..38471)
                     /gene="mutT"
                     /locus_tag="OO464_000863"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6226109.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38531..41236)
                     /gene="secA"
                     /locus_tag="OO464_000864"
     CDS             complement(38531..41236)
                     /gene="secA"
                     /locus_tag="OO464_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6226110.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41298..41810)
                     /gene="secM"
                     /locus_tag="OO464_000865"
     CDS             complement(41298..41810)
                     /gene="secM"
                     /locus_tag="OO464_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6226111.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42041..42958)
                     /gene="lpxC"
                     /locus_tag="OO464_000866"
     CDS             complement(42041..42958)
                     /gene="lpxC"
                     /locus_tag="OO464_000866"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6226112.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43059..44210)
                     /gene="ftsZ"
                     /locus_tag="OO464_000867"
     CDS             complement(43059..44210)
                     /gene="ftsZ"
                     /locus_tag="OO464_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6226113.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44271..45533)
                     /gene="ftsA"
                     /locus_tag="OO464_000868"
     CDS             complement(44271..45533)
                     /gene="ftsA"
                     /locus_tag="OO464_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6226114.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45530..46360)
                     /gene="ftsQ"
                     /locus_tag="OO464_000869"
     CDS             complement(45530..46360)
                     /gene="ftsQ"
                     /locus_tag="OO464_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6226115.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46362..47282)
                     /gene="ddlB"
                     /locus_tag="OO464_000870"
     CDS             complement(46362..47282)
                     /gene="ddlB"
                     /locus_tag="OO464_000870"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6226116.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47275..48750)
                     /gene="murC"
                     /locus_tag="OO464_000871"
     CDS             complement(47275..48750)
                     /gene="murC"
                     /locus_tag="OO464_000871"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6226117.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48804..49871)
                     /gene="murG"
                     /locus_tag="OO464_000872"
     CDS             complement(48804..49871)
                     /gene="murG"
                     /locus_tag="OO464_000872"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6226118.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49868..51112)
                     /gene="ftsW"
                     /locus_tag="OO464_000873"
     CDS             complement(49868..51112)
                     /gene="ftsW"
                     /locus_tag="OO464_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6226119.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51112..52428)
                     /gene="murD"
                     /locus_tag="OO464_000874"
     CDS             complement(51112..52428)
                     /gene="murD"
                     /locus_tag="OO464_000874"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6226120.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52431..53513)
                     /gene="mraY"
                     /locus_tag="OO464_000875"
     CDS             complement(52431..53513)
                     /gene="mraY"
                     /locus_tag="OO464_000875"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6226121.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53507..54865)
                     /gene="murF"
                     /locus_tag="OO464_000876"
     CDS             complement(53507..54865)
                     /gene="murF"
                     /locus_tag="OO464_000876"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6226122.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54862..56349)
                     /gene="murE"
                     /locus_tag="OO464_000877"
     CDS             complement(54862..56349)
                     /gene="murE"
                     /locus_tag="OO464_000877"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6226123.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56336..58102)
                     /gene="ftsI"
                     /locus_tag="OO464_000878"
     CDS             complement(56336..58102)
                     /gene="ftsI"
                     /locus_tag="OO464_000878"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6226124.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSVLVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58118..58483)
                     /gene="ftsL"
                     /locus_tag="OO464_000879"
     CDS             complement(58118..58483)
                     /gene="ftsL"
                     /locus_tag="OO464_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6226125.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58480..59421)
                     /gene="rsmH"
                     /locus_tag="OO464_000880"
     CDS             complement(58480..59421)
                     /gene="rsmH"
                     /locus_tag="OO464_000880"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6226126.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59423..59881)
                     /gene="mraZ"
                     /locus_tag="OO464_000881"
     CDS             complement(59423..59881)
                     /gene="mraZ"
                     /locus_tag="OO464_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6226127.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14473 a        16734 c        14849 g        14365 t
ORIGIN      
        1 caaaggttgg tactgaaaac gggcaactcc ttggtaatac tctcacaggt aatgatgcag
       61 caaaaggagt cggtgtactc atagaaggtt tagcaactag taaaaatcct ctaatgacat
      121 tgaaacctaa tgattcaaat tctgtttata aagattacga cccgagaggc aaagacgata
      181 caacaggagg agtttacccg gatcaagata caggtataac ataccctctc catttccagg
      241 ccacgctaca acaggatgga actataccaa tagaagctgg tgagtttaaa gccaccagta
      301 ctttccaggt aacctaccct taataagtcc accgcaccgc catcatctgg cggtgcggta
      361 attgataaat cattgccccc accaatgtga ctgataaaat gccaggcaac acgacatcat
      421 ttatcaggat acgttatgaa accgaccacc attgcttcac ttcagaaata taagcaggat
      481 aaaaagcgtt tcgcgaccat caccgcttac gactacagct tcgcgaaact atttgctgat
      541 gaagggctta acgtcatgct ggtaggcgac tcgctgggca tgaccgttca gggccacgat
      601 tccactctgc cagtaaccgt tgctgatatc gcctaccaca ctgccgccgt acgtcgcggc
      661 gcaccaaact gcctgctatt ggctgacctg ccgtttatgg cgtatgccac gccggaacaa
      721 gcctttgaaa acgccgcaac ggttatgcgt gccggtgcca atatggtcaa aattgagggc
      781 ggtgagtggc tggtcgaaac cgtaaaaatg ctgaccgaac gtgccgttcc tgtatgtggt
      841 cacttaggtt taaccccaca gtcagtgaat attttcggtg gctacaaagt acaggggcgc
      901 ggcaatgaag cgagcgatcg actactcagc gatgcattag ccttagaagc cgcgggggca
      961 cagctgctgg tgctggaatg cgtgccggtt gagctggcaa aacgtattac cgaagcactg
     1021 gcgatcccgg ttattggtat tggtgcaggc aacgtcactg acgggcaaat cctcgtgatg
     1081 cacgacgcct ttggtattac cggcggtcac attcctaaat tcgctaaaaa tttcctcgcc
     1141 gaaacgggcg acatccgcgc ggctgtgcgg cagtatatgg ctgaagtgga gtccggcgtt
     1201 tatccgggcg aagaacacag tttccattaa ggagtcacgt tgtgttaatt atcgaaaccc
     1261 tgccgctgct gcgtcagcaa attcgccgcc tgcgtatgga aggcaagcgc gtggcgctgg
     1321 tgcctactat gggtaatctg cacaatggcc atatgaagct ggtcgacgaa gccaaagccc
     1381 gcgccgatgt ggtcgtcgtc agtattttcg ttaacccgat gcagttcgac cgcccggaag
     1441 atctggcacg ttatccacgg accttgcagg aggactgcga gaagctaaac aaacgtaaag
     1501 tggatttagt tttcgcccct tcggtaaaag agatctaccc gaacggtact gaaacccaca
     1561 cttacgttga cgttcctggc ctttcgacca tgctggaagg tgccagccgt ccgggacatt
     1621 ttcgcggcgt ttcgactatt gtcagcaagc tgttcaacct ggtccagccg gacatcgcct
     1681 gcttcggtga aaaagatttt cagcaactgg cgttgatccg caaaatggtt gccgatatgg
     1741 gcttcgatat tgagattgtc ggtgtgccaa ttatgcgcgc caaagacggt ctggcgctaa
     1801 gttcccgtaa cggttatctg acggcggaac aacgcaaaat tgcgcctggt ctgtacaaag
     1861 ttttaagttc gattgctgac aaattgcagg ctggggaacg ggatctcgat gaaattatta
     1921 ccattgcggg gcaagaactg aatgaaaaag gcttccgcgc cgatgatatt cagattcgcg
     1981 atgccgacac attgctggaa gtttctgaaa ccagcaaacg ggcagtaatt ctggtagccg
     2041 cctggcttgg cgatgctcgc ctgatcgaca acaaaatggt cgagctggcg taatacttaa
     2101 ctggcgctac ggctgatggc gccagttatt aatttacccc acgtcatctg tcataacaca
     2161 ggtaacttaa ataaggttaa ttaccttatc aatctcagca agaggtaaat ttgccatctc
     2221 tgcaacgtct tcccgagaca ttcctttact tagaagacgc agggcgaatt cttgcctttc
     2281 ctcctgtctt ccctgctgaa tccccttctc aatccctttc tcttcaaacc actgcgccag
     2341 cgtcatcata gacttccctc ccgtttccct gtctctcagc acaccataaa acaaatccgc
     2401 ttgttcagta tgaccgcgtt gcagcatata gttttgcatg gcaactaact gacttccgct
     2461 agtgtaccct tcgtcaatca gcgtgaccag ttgctccagc aataacatta agtcgcgctg
     2521 gcgaatatgt ttttgcagta gttcgagaat cgcaatccgc cgatgttgca tgatttcgtc
     2581 atccggtgtg atggtgatat ccaccagcgg gaaaggactg ttatagacgc gtcgcgccag
     2641 ctccggcgag taaaacatat caaaccagca cattgagagc ggataaggtg tggcctcgcc
     2701 ctgataaaac agtatcggca ccaccagcgg cagcttatcg tggccagcct ccagatgccg
     2761 gtgcatggca gcaatagaat aacgcatcat gcgaaaggcc attttcttat ccggcttgct
     2821 ttggtgttca atcacaacat gcagataacc gggattgccc tgcatttgca tggaatagag
     2881 cacgtccgtg ctgtgacctt tcaggcactc ttcaatgaaa ctccccgact ctaaatgaag
     2941 cgtgttgaga tcacaaagtt cgcgtaattc cactggcaaa tgtatctcca gaaagtcgcg
     3001 agccgtctcc gcatgcatta aaaattgttt aaataccgcg tcatgcggtg tggtactcgg
     3061 tgcatccatc ttccctgtct tccgtgtggc cttgcgtgaa ggcagtatcc agtcaaggcg
     3121 tgctatcgtc agccacctct ttattgttta ccgagcagcg ttcataaaaa tatcaattcg
     3181 ttacaggcgt tacatggcac gcttcggcgc ggtacgcaaa ctgctgacgc aaatgccatc
     3241 agatgagtag acactcatca aaaatcgggc aatactgcgt gagaatttcc tcaaagcagg
     3301 caccgcctgc ttcgttaacg acagggtaga aaggtagaag ttatgattcg cacgatgctg
     3361 cagggcaaac tccaccgcgt gaaagtgact catgcggacc tgcactatga aggttcttgc
     3421 gccattgacc aggattttct tgacgcagcc ggtattctcg aaaacgaagc catcgatatc
     3481 tggaatgtca ccaacggcaa gcgtttctcc acttatgcca tcgcggcaga acgcggttcg
     3541 agaattattt ctgttaacgg tgcggcggcc cactgcgcca gcgtcggcga tattgtcatc
     3601 atcgccagct tcgttaccat gccagatgaa gaagctcgca cctggcgacc caatgtcgcc
     3661 tattttgaag gcgacaatga aatgaaacgt accgcgaaag cgattccggt acaggttgct
     3721 tgattgttat ccctgcggct ggttactcac cagccgcgac atcttctcca gcgaatccgt
     3781 tcttaagata taaagtcgtt ttagtaacaa cggattatcc cccggtttta ctttgccttt
     3841 catggttgtc accgccaggt gaaatccggc atcgtttgct gccttcacgg ctttgtcatt
     3901 aaagccgcca aacggatacg aaagatacca gacatgtgga ttaaattgcg ccagagcgcg
     3961 gcgtgaacgt gcaaaatcaa acagaatatt gtgctcacta cggctcagta atatgggtcg
     4021 gcgataacca tctacccgat gcaaaaaatg ggtatgtgac tggaaatcaa aaacatcgcg
     4081 aatttcgtta agctcagaaa cgctcataaa ttgcagcgat tttgggttcc acttctgcgg
     4141 gtgacgtttg atgcgtgagg taacaataaa caccgtcgcc ttcatgccat attgtttcaa
     4201 cacaggatac gcatagcggc tcaccgactt gaggccatca tcaaaggtaa tcaccaccgc
     4261 tcgcgcaggg agattgatcg tattcttcac gtaaccttcc agctgagcca tgctcagtgt
     4321 cgcgtatccc ctgtcgcgca gccaggccat ttggttattg aaagcgcgta ccgaggtggt
     4381 cgtcgaagta tggcgaaaac gggtgttttc ttcgtcgcgc agaatatggt gataagttag
     4441 caccggcagg ccattatcgg gttgggcatc cagcgcgctg atataggcca gtcgatcgcc
     4501 aatacggatc tgataccagg tttgatttaa cctgtctttc agtttatgca aaatcgggta
     4561 gcgcaaattg tccgccagta ccccaaatgg cgcactgccc acactcggcg cgttgtagac
     4621 cggcgtatct ttccaggtaa ttaagttctg attactcagc ggcttgttga ggtcacccaa
     4681 accgtcttca actttttgtc gtccctgaac tggctcgaga tgccctttat cgataaaccc
     4741 tttaccaaag ccaaaattaa atgcgtaata acttgcggca gtgggctcca cggcaatgat
     4801 ttgtccggcc cgaatattcc ccacggtcac catcttgtca ctaatttgcg cccagaccgc
     4861 agcattttcg atggtttgca tataacgggc aggtaacgcg gcactgacac ttgcggtaaa
     4921 cagcatcagc aggagaataa cagcttgttt atacatgaaa aaataaccga gggtgaaaca
     4981 acgcttttat tgtagcaaaa gcgccatcag aatcgcgggt taatactcat acaaattatg
     5041 tttgaggacg aaaggcggat ttttttgttg ttggtgccaa agactactca cctccggccc
     5101 acccagttcg acaatacact cacgaaactc tgaactggtc atggtttcac ggcaagccat
     5161 catctgttca attaacggta acgtgacacc agaaatcact tcgcaacggg agtgtttgtg
     5221 gcttaataat gaagccacgc gatacggtgg cgctccggct atatcggtta agaagatgac
     5281 accctcacca gagtccgctt catgcagagc atcgcacatc atgcggctga gcatattaga
     5341 gcttaatccg cgccagaaat tcacggcccg gcactgaaga agtgccccat gttttttctc
     5401 cagcgcatcc agtatctctt gcgcccggtc atcgtgacag gtaattaccc aacctaacat
     5461 cgccttcctc cttagccgaa aatcagccta ccctgaccat tgttgataaa gggtgatagc
     5521 cgtcaaaact cctctccccc gaatccagag gagaggaaat agccttagct acgcaaacca
     5581 cgtccacgtt ggatcagcga ccaacagatc aaataaaacg ccacaataaa gaccaccagt
     5641 acgccaaagg tagtgaccag cggcacatca tttataccga ggaagccgta gcggaaacca
     5701 ctgatcatat aaacgattgg gttcaggtgc gacagccctt gccagaacgg cggcaacaaa
     5761 gtcagtgagt aaaagacccc gcccaaatac gtgagtggcg ttaacacaaa ggttggcacc
     5821 aggctgatgt catcgaacgt tttggcaaac acgccgttca gcaaacccgc aagggagaat
     5881 agcaccgccg tgagcaccag ggttaaggca acgaataccc acgaatgcac ctgaaacggc
     5941 acaaaaaaca gtgaaattgc cgtcacaaga atgccaacaa acagaccacg cgccacgcca
     6001 ccgccgacat atccggcaat aatgacgtga gtcggaaccg gcgctaccag cagctcttca
     6061 atattacgct ggaacttggc accaaaaaat gatgacgcga cgttagcgta ggcgttggtg
     6121 atcaccgaca tcatgatcag ccccggtacg atgaactgca tatagctgaa gccatgcata
     6181 tcgccaatac gcgaacctat caggttaccg aagataataa agtaaagggt catggtgatg
     6241 actggcggca ccagcgtctg cacccagata cgcataaagc gatggatctc tttcgcccag
     6301 atgcttttta gcgccaccca gtaaagatgc atcatgcgcg atctccttgt ttttcattaa
     6361 ccagtgaaac aaacagctct tccagacggt tagctttgtt acgcatactt aatacctgaa
     6421 tgccctgctc acttaactgc gtaaatacgc tgttgatccc ctgctcacgt agcacttcaa
     6481 cttccagcgt cgcggtatcg accagtcgat actgatagcc atcgagcttc ggtaacggac
     6541 ttttcggtgc gagatcgaga ataaaggttt ccgatttcag cttcgccagc agcgccttca
     6601 tcgaggtatt ttccaccagc tcaccatgtt gaataatgcc gatattgcgg cacagcattt
     6661 ctgcttcttc caggtagtgt gtggtaagaa tgatggtggt gcctttgtcg tttaaatcct
     6721 tcaaaaagcc ccacattgag cggcgaagtt caatatccac gcctgcggtc ggttcgtcga
     6781 gaatcagtag tttaggttca tgcattaacg cacgggcaat cattaaacgg cgcttcatcc
     6841 cgccagataa catacgcgca cgttcgttgc gttttcccca taaatcgagc tgcttaagat
     6901 atttttcgct gcggatgtac gcctctttgc gctccacacc gtagtaccct gcctgattca
     6961 ccacaatttg ctgcacggtt tcaaacgggt tgaagttaaa ttcctgcggc accagtccca
     7021 actgacgttt agcgttcacg acatctttct cgagatcgta accaaatacg ctgacccgcc
     7081 cggaggtttt atttaccaga gagctgataa taccgatagt ggtcgatttc ccggccccgt
     7141 tcggcccgag aagcgcataa aaatcacccg cttcgacctg caaatctatt ccacgaagcg
     7201 cctgaacgcc gcctggatag ttttttttaa gctgttgaag ttccagtgca atggtcataa
     7261 atttttactt accttacgtt cttacacttt atctgtggtt taaatcgtcc cggagttgcc
     7321 ctatattagc caaacgtaat tatttggtta caggtcgtta acctccatga aagacataga
     7381 tacactcatc agcaacaatg cactatggtc aaaaatgctg gtggaagagg atcccgggtt
     7441 ttttgagaaa ctggcacaag cgcaaaaacc gcgctttcta tggattggat gttccgacag
     7501 tcgcgttcct gcagaacgtt taaccggtct tgagccgggc gaactctttg ttcaccgtaa
     7561 tgttgctaac ctagtcattc acaccgacct gaactgcctt tccgtggttc agtatgcagt
     7621 ggatgtactc gaagttgaac acattattat ctgtggccac tacggttgcg gcggcgtaca
     7681 agccgccgtt gaaaacccgg aactggggct tatcaacaac tggctgctgc atatccgcga
     7741 tatctggttc aaacatagct cattgctcgg cgaaatgccg caagagcgcc gtctggatac
     7801 cttgtgtgaa ctgaacgtca tggaacaggt gtataacctg ggccactcca ccattatgca
     7861 atcagcgtgg aaacgcgggc agaaagttac cattcacggc tgggcctacg gcattcacga
     7921 cggcttgctg cgtgatctgg atgttaccgc caccaaccgc gaaacccttg agcaacgtta
     7981 ccgtcacggg atttccaacc tcaagctgaa acacgccaac cacaaataaa aatgccatgc
     8041 cggatgcaac acatccggca acttcacact tactcgtcca gcagaatcac tttgccaata
     8101 tacggcagat gacggtaacg ctgtgcgtaa tcaatgccgt aacccaccac aaactcatcc
     8161 gggatcgaga aaccgataaa ttcaaccggg acgttcactt cacgacggga cggtttatcc
     8221 agcagcgtac aaatcgccaa cgacttcggt tcgcgcaggc ttaagatctc acgcactttc
     8281 gacagtgtat tccccgagtc gatgatatct tcaacaatca gcacgtcctt gccacggata
     8341 tcttcatcca gatctttgag gattttcaca tcacgggtgg tggacatgcc gctaccgtag
     8401 ctggaggcgg tcataaagtc gacttcatga gatacctgaa cttcacggca caggtccgcc
     8461 ataaacataa atgagccacg cagcagaccc accagcacca tatcgctgcc gctgtctttg
     8521 taacgctcag taatctgacg acccagttcg gcgatacgcg ctttaatctc cgcttcgggg
     8581 atcattactt ctacagtatg tttcatatct ctaaccatat gatttaaaaa caaatcactt
     8641 cgcacgggca ggttttggca ccgcaacaaa gcgcaagacg cgcagtatac cagtaaaact
     8701 attcattcgc ggaatctgtt aataaagctc gaattgtgat gacggtcaca catgttaaaa
     8761 cctataatta attgctacta aaatattaat gaattgaaat ggtgtctctt tatggcaatt
     8821 aacaatacag cctcgcgacg attactcgtc acgctaacag ccctttttgc agcgctttgc
     8881 gggctgtatc tactcattgg cggaggctgg ctggtcgcga ttggcggctc ctggtactac
     8941 cctatcgctg gccttgtgat gctcggcgtc gcctggatgc tgtggcgcag taaacgcgcc
     9001 gcgctttggc tatacgcagc cctgctgctc ggcaccatga tttggggcgt ctgggaagtt
     9061 ggtttcgact tctgggcgct gactccgcgc agcgatattc tggtcttctt cggcatctgg
     9121 ctgatcctgc cgtttgtctg gcgtcgcctg gtcattcctg ccagcggcgc agttgccgca
     9181 ctggtggtcg cactgctgat tagcggtggt atcctgacct gggccggatt taacgatccg
     9241 caggagatca acggcacctt aagcgccgat gccacacctg ccgaagctat ctcccccgtt
     9301 gccgatcagg actggcctgc gtatggccgc aatcaggaag gtcaacgctt ttcaccactg
     9361 aagcaaattc acgccgataa cgtccacaag ctgaaagaag cctgggtgtt ccgtactggc
     9421 gatgtgaagc agccgaacga tccgggtgaa atcaccaatg aagtgacgcc gattaaagtg
     9481 ggcgataccc tttacctgtg taccgctcac cagcgcctgt ttgcgcttga tgccgccagc
     9541 ggcaaagaga aatggcatta cgatcctgag ctgaaaacca acgagtcttt ccagcacgta
     9601 acctgccgtg gcgtctctta tcatgaagcc aaagcagaaa cggcttcgcc ggaagtgatg
     9661 gcggattgcc cgcgtcgtat cattcttcca gtgaatgatg gtcgcctgat tgcgattaac
     9721 gctgaaaacg gcaaactgtg cgaaaccttc gccaataaag gcgtgctcaa tctgcaaagc
     9781 aatatgccag acaccaaacc gggtctgtat gagccgactt ctccgccaat tatcaccgat
     9841 aaaaccatcg tgatggccgg ttcagtcacc gataacttct caacccgcga aacgtctggc
     9901 gtgatccgtg gttttgatgt caacaccggg gagctgctgt gggcttttga tccgggcgcg
     9961 aaagatccga acgcaatccc gtcagacgaa cacaccttta cctttaactc accaaactcg
    10021 tgggcaccag cggcctatga cgcgaagctg gatctggtct atctgccgat gggcgtgacc
    10081 acgccggata tctggggcgg taaccgcaca ccggaacagg aacgttatgc cagctcgatt
    10141 ctggcgctga atgccactac cgggaaactg gcgtggagct accagaccgt tcaccacgac
    10201 ctgtgggaca tggatcttcc ggcacagccg acgctggcgg acatcaccgt taatggtcag
    10261 aaagtgccga ttatttacgc tccggcgaaa accggtaaca tttttgtgct cgatcgtcgt
    10321 aatggcgaac tggtggttcc ggccccggaa aaaccggttc cccaaggtgc tgccaaaggc
    10381 gattacgtta cccctactca accattctcg gaactgagct tccgtccgac gaaagatttg
    10441 agcggtgcgg atatgtgggg agccaccatg tttgaccaac tggtgtgccg cgtgatgttc
    10501 caccagatgc gctatgaagg cattttcacg ccgccatctg aacagggtac gctggtcttc
    10561 ccgggtaacc tggggatgtt cgaatggggc gggatttccg ttgatccaaa tcgtgaagtg
    10621 gcaattgcca acccaatggc actgccgttt gtttcgaaac tgctcccgcg tggtcctggc
    10681 aacccgatgg agcagccgaa agatgccaaa ggcacgggta cggaatccgg cattcagcca
    10741 cagtacggtg taccgtatgg tgtcacgctc aacccgttcc tctcaccatt tggtctgcca
    10801 tgtaaacagc cagcatgggg ttatatctcg gcgctggatc tgaaaactaa tgaagtggtg
    10861 tggaagaaac gtattggtac gccgcaggac agtatgccgt tcccgatgcc ggttccggtg
    10921 ccgttcaata tgggtatgcc gatgctgggc gggccaatct ccacggcggg taacgtgctg
    10981 tttatcgccg ctacggcaga taactacctg cgcgcttaca acatgagcaa cggtgaaaaa
    11041 ctgtggcagg gtcgtttacc ggcgggtggt caggcaacac cgatgaccta tgaagtgaat
    11101 ggcaagcagt acgtggtgat ctccgcaggc ggtcacggtt catttggtac gaagatgggc
    11161 gactatattg tggcttatgc cctgccggat gatgtgaaat aaccataatg cccggagaga
    11221 tccgggcata tttccgaata cggtcttttt ataccgtaaa ccctaacatc atccccgtat
    11281 cttcatgctc cagcagatgg cagtgcgcca tataagcatg ttctttcggt gcgttgtgat
    11341 taaacttcac cagcacttca ctgacattgc cttctacctt aacggtatct ttccagcccg
    11401 cgcgatgagc cgctggcggt ttgccatttt ctgacaagat acggaactgc gtgccgtgaa
    11461 tatggaacgg atgcagcatc atgtcgccca cgccagagat aacccaacgt tcgtactgcc
    11521 ctttcgccgc cgcaaacatc ggcttattca tatcaaacgc ctgaccgttg attttattgg
    11581 catggtggaa atcgaacttc ccgccgtggt tcatatgatt catattgccg tgccccatat
    11641 ggcccatcat ctggctgtga tccattccgg ccatcgcctg atcgccatat ttctccatta
    11701 gcatctgcat ccccatcata tcgagcatcg ggtccataga aagttgcagc ttgcgtaccg
    11761 tcagcccttc cagcgaaggt aacgcaggca ggctacttaa tgtgtcaggc aaagcaccgg
    11821 aggcactaat agcaatcggc tgaatccgca ttaccggatg cggcttgtca aacggtgcaa
    11881 tcgccatccc catctggctg accggcagcg tcaccaggtc aaagggtttg ttgtcgttaa
    11941 cctccaccag cacttcaaaa cgctcgccca tcagcaccgg cagctcgctc accttcaccg
    12001 gttcaggtag cagaccaccg tcgctggcaa tcacatacag cgggcgattg tcgctggtgg
    12061 cgaaattgag cgagcgggca ttacagccat tgagcaaacg caggcgcagc caaccacgcg
    12121 gggcagcgtg ttgtgggtag attgcaccgt tggtcagcag cgtatcgcca aaccagccca
    12181 cggcggcggt catcacatcc agttgataat caatctgccc gtcggcgcta aatttcttat
    12241 cctgaacgat caccggaaca tcatcgatac cccactgttt tggcagcatt aatttcagga
    12301 tctcgtcatc ttcaatcacc accagaccag ccagccccat cgccacctgt cgcccggtct
    12361 tgccatgttg atgtggatgg aaccagcagg tagcggcagg ttgatcaacg ttcaacgtca
    12421 ccgagcgctt gccacctggc ggaataattc cctgtgggcc gccgtcgacg tcacccggta
    12481 cttccagccc gtgccagtgc aacgtcgtct cttccgccag ttggttgtag atatcaaccg
    12541 ttaccgcttt gccgcgctgt aatttcaccg ccggccccag cagattgccg ttatagcccc
    12601 aggtagttgc agttttctcg ccaaaggtgg actgacctgc gccaatagtt aactgaatgc
    12661 gattacgggc atcggtcgtg agcaaatcag ggatcggtaa cgttgggcgt tccgccgcaa
    12721 agactgcacg gctccacagt ggcaaggctg aagccacacc cagtgcgacg gaatatttca
    12781 agaaatcacg acgttgcata gttatttcct tattcttaag caggcgaaac aaaatcaaac
    12841 gttgagcata gtccttcccc ttacgggaag gtcaagccgc agacacaatt taatcgacaa
    12901 taatggtcgt cgcctcgctc gcagtgtgct aacgtttatc ttctttaagc ccctggtaga
    12961 agcaatgaag acgtttttca gaacagtgtt attcggcagc ctgatcgccg tttgcgcaaa
    13021 cagttacgcg ctcagcgagt ctgaagccga agatatggcc gatttaacgg cagtttttgt
    13081 ctttctgaag aacgattgtg gttaccagaa cttatctaac gggcaaattc gtcgcgcact
    13141 ggtctttttc gcccagcaaa accagtggga tctcagtaat tacgacacct tcgacatgaa
    13201 agctctcggt gaagacagct accgcgatct cagcggcatt ggcattcccg tcgctaaaaa
    13261 atgcaaagcc ctggcccgcg attccttaag cctgcttgcc tacgtcaaat aatccctgat
    13321 acctttttgt agaaatattg accgtgcatc cgcggtcaat gttagctatt atgttgcgcc
    13381 ctttttttac gggtgttaac aaaggaggta tcaacccatg gccgaaaaaa aacagtggca
    13441 tgaaacgcta cacgaccagt ttgggcagta ctttgcggta gataacgttc tgtatcatga
    13501 aaagaccgat caccaggatc tgatcatttt tgagaacgct gcatttggtc gcgtaatggc
    13561 gctggatggc gtagtacaaa ccaccgagcg cgacgagttt atctatcatg agatgatgac
    13621 ccatgttccg ctactggccc acggtcacgc gaaacatgtg ctgattatcg gcggcggcga
    13681 cggtgccatg ctgcgtgaag taacccgaca taaaaacgtt gagtcaatca cgatggtgga
    13741 aatcgatgcg ggtgtcgtgt cgttctgccg tcagtatctg cccaaccata acgccggtag
    13801 ctacgacgat ccgcgctttc aactagtgat cgacgatggc gtcaatttcg ttaatcaaac
    13861 cagccagacc tttgatgtca ttatctccga ctgcaccgat cctatcggtc ccggcgaaag
    13921 cctgttcacc tcagcatttt atgaaggctg caaacgttgc ctgaatcctg gcggtatctt
    13981 cgtcgcacaa aacggcgtct gctttttaca gcaggaagaa gccatcgaca gccatcgcaa
    14041 actcagccat tacttcagcg acgttggctt ttatcaggcg gcgatcccga cctattacgg
    14101 cggtatcatg acttttgcat gggcgacaga taacgacgcc ttacgccatc tctcaaccga
    14161 aattattcag gcgcgttttc tcgcttctgg cctgaaatgc cgttattaca atccggcaat
    14221 ccatacggca gcttttgcct tacctcagta tctgcaagac gcactggctt cacagccgtc
    14281 ctaaggagaa gataagaaat tgaaaaaact gaaactgcat ggctttaata atctgaccaa
    14341 aagtctgagt ttttgtattt acgatatctg ctacgccaaa actgccgaag agcgcgacgg
    14401 ttatattgct tatatcgatg aactctataa tgccaaccgt ctgaccgaaa tcctgtcaga
    14461 aacctgttcc attatcgggg ccaatattct taacatcgcc cgccaggatt acgaaccaca
    14521 gggtgccagc gtcactattc tggtgagtga agaaccggtt gacccgaaac tcatcgacaa
    14581 aacagaacac cccggcccac tgccagaaac ggtggttgct catctcgata aaagtcatat
    14641 ttgcgtacat acctacccgg aaagtcatcc tgaaggcggt ttatgtacct tccgcgccga
    14701 tattgaagtc tctacctgcg gcgtgatttc tccgctgaag gcgctgaatt acctgatcca
    14761 ccagcttgag tccgatatcg taaccattga ttatcgcgtg cgcggtttta ctcgcgacat
    14821 taacggtatg aagcacttta tcgaccatga gattaattcg attcagaact ttatgtctga
    14881 cgatatgaag gcgctgtatg acatggtgga tgtaaacgtc tatcaggaaa atatcttcca
    14941 taccaagatg ttgcttaaag agttcgacct taagcactac atgttccaca ccaaaccgga
    15001 agatttaacc gacagcgagc gccaggaaat taccgctgcg ctgtggaaag aaatgcgcga
    15061 gatttattac gggcgcaata tgccagctgt ttaacggctc tggcggagct cccaggctcc
    15121 gccagattta tttacttctg ctgcacgaaa ttgcggtaag ccgccacgac ctgcagaaaa
    15181 tcctcaacgc cgcatagcga caggctttct tcgtcgtagt agttcatccc ctcttccatt
    15241 tcatcgccag cgaattccag ttgattggcg cgaaccatca cctcttcagc gtccatccac
    15301 agggtgtatt catgccctgc ccgttgccag gaccgttcgc tacctttcag tgcgtgcgct
    15361 gcttgttcca cttcatcaag caaggccagg ttttctttca cctcttcatt aaaccagtgc
    15421 ccgaccactt catgccccat ggacatacgc acctttacca ctccggtaat atcgcgcaga
    15481 aattcgtaat ccatagtgtg ttcctctgct cccggcaatg cgccgaaatc gcatcgcgcc
    15541 attgatgtaa ttatcgcagc cttaccggag aaaaacagcg gaacggcgat agtttagaaa
    15601 taaaaaatgc ccgggaatct cattcaccgg gcattgtgtc gtttatgcgc agcgcgtgcg
    15661 cttacttatt acaccgcagt ctggaaaatc accccatcgg ctttttcggt gtactgagaa
    15721 agctggttga agttcagata acgataagta tcaacggcag ttttatctac ttgcgcaacg
    15781 taggtctggt actcttccgg cgtcggcagt ttgccaatca gcgccgcaac agccgccagt
    15841 tctgcagaag ccaggaagac attcgcgcca gtacccaaac ggttcgggaa gttacgggta
    15901 gaggtggaaa ccaccgtcgc accgtctgct acacgcgcct ggttacccat acacagggaa
    15961 cagcccggga tctcgatacg agccccgctc ttaccgaaga cgctgtagta gccttcttcc
    16021 gtcagttgcg cggcgtccat acgggttggc ggtgccaccc acaggcgggt cggcaactga
    16081 cctttgtgcg catccagcag tttaccagcg gcacggaagt gaccgatgtt agtcatgcaa
    16141 gaaccgataa atacttcgtc gatcttctcg ccctgtaccg cagacagcgg acgtgcgtca
    16201 tccggatcgt tcggagcaca caggattggc tctttaatct ccgccagatc gatgtcgatc
    16261 actgccgcgt attccgcatc tgcatcggct tccagcagct caggattcgc cagccatttt
    16321 tccatgccct gaatacggcg ttccagggta cgacgatcgc cgtaaccttc cgcgatcatc
    16381 cacttcagca ggacgatgtt ggagctcagg tattcgatga tcggttcttt gttcagcttg
    16441 atagtacaac cagcggcaga acgctcggcg gacgcatcgg ttagctcaaa ggcctgctca
    16501 actttcagat ccggcagacc ttcaatttcc agaatgcgac cagagaagat gtttttcttg
    16561 cctttcttct caacggtcag cagaccttgt ttgatcgcat acagcggaat ggcgtgtacc
    16621 agatcgcgca gggtgatgcc cggctgcatt ttgcctttga agcgcaccag aacggattcc
    16681 ggcatatcca gcggcattac gccagttgcg gcagcaaacg ccaccagacc agaacccgcc
    16741 gggaaagaga taccgatcgg gaaacgggta tgggagtcac caccggtacc gacggtatcc
    16801 ggcagcagca tacggttcag ccaggagtga atgacgccgt cacccggacg cagcgacaca
    16861 ccgccacggt tcataatgaa gtccggcagc gtgtggtgcg tgttcacgtc aactggcttc
    16921 ggatacgccg cggtgtggca gaaagactgc atcaccaggt cagccgagaa gcccaggcac
    16981 gccaggtctt tcagttcatc acgggtcatc gggccggtgg tgtcctgaga gcctacagaa
    17041 gtcattttcg gttcgcagta cgcgcccgga cgaatgcctt tcacgccaca ggcacggcct
    17101 accattttct gcgccagcga gaagccgcga tcgctctcag cgacatcttt cgcctgacgg
    17161 aacacatcac tgtgcggcag accaagtgct tcacgcgctt tggtggtcag gccacgcccg
    17221 ataatcagcg gaatacggcc gccagcacgc acttcatcaa tcagcacgtc ggttttcagt
    17281 tcgaaggtcg ccagcagttc gcctgtttcg tggttacgca cttcaccttt gtacgggtaa
    17341 acgtcaatca cgtcgcccat gttcaggtta gagacgctga cttcgattgg cagtgcaccc
    17401 gcgtcttcca tcgtgttaaa gaagatgggt gcaattttac cgccgaggca caaaccaccg
    17461 ccgcgtttgt tcggcacatg tggaatatca tcgcccataa accacagaac ggagttcgtg
    17521 gcggatttac gcgatgaacc cgtacccaca acgtcaccga cgtacgccag cgggaaacct
    17581 ttctgttgca gagcttcaat ctgtttgatc gggccaacaa cacctggctg gtctggctca
    17641 ataccttcac gggcgttttt cagcatcgcc agcgcgtgca gtgggatatc cgggcgtgac
    17701 cacgcatccg gtgccggaga aaggtcatcg gtgttggttt cgccagtgac tttaaagacg
    17761 gtaacggtca gtttttcagc cagcgccggg cgattcagga accattcggc atccgcccag
    17821 gactgcatta cctgcttcgc atattcgttg cctgctttcg ctttctcttc tacgtcatag
    17881 aagttatcga acatcagcag cgtgtgagaa agtgctttgg cagcaatagg tgccagtttg
    17941 gcatcatcca gcgcgtcgat cagcggatga atgttgtaac caccctgcat ggtgcccagc
    18001 agttcgatag ctttttccgg agtcagcaga ggggatttag cttcgccttt agcgatagca
    18061 gccaggaagc ctgcttttac ataggcggct tcatcgacgc ctgggggaac acggttggtt
    18121 aacagatcta acaggaattc ttcttcgccc gcgggcgggt ttttcagcag ctctacaagc
    18181 gcggccattt ggtttgcatc caggggtttg ggcgcaatcc cctcagcggc acgctcagct
    18241 acgtgcttac ggtattcttc tagcacgacg gttctcctcg ctctcattgt catagtgcgg
    18301 caggcgattc tcttcacgct cctgtgagac agcagtttgt agggtaaatg cccagtaccg
    18361 caacgggcag aatagcagga ttttgacgac gtgttaatct gtttacaaaa aagcaacata
    18421 aaaaagtggc tgaatcgtta aggatggtct agagattgtt tccctccgta aaaacttcgt
    18481 taattcgcat ggtgataatc acctttctca acgaatccca agattagtca aaatttaaac
    18541 taccgcctct ttatactcgg attcacagca cctgcgggtg gcagttcgcc gaccattgcg
    18601 atttccttga gatccgaatt atgaaaatga ctttgccgtt taaaccccat gtgctggcgc
    18661 taatttgcag tgccgggctt tgtgctgcct ctgccgggct atatataaaa agccgcacag
    18721 tggaagcgcc tgtagaaccg caatcgacac aactggctgt gtctgacgct gccgctgtta
    18781 cgcttcctgc aacggtttcc gcacctcccg taatacccgc agtcgtcaaa tccgcattca
    18841 gcactgcaca aatagatcaa tgggtcgcac ccgtcgcgct gtatcccgac gccctacttt
    18901 cgcaggtgct gatggcatca acctatccga caaacgttgc tcaagcagtg caatggtcgc
    18961 acgataatcc acttaaacaa ggcgatgctg ctattcaggc ggtatctgac cagccgtggg
    19021 acgccagcgt taaatcactg gtggcctttc cacaattgat ggcattgatg ggcgaaaacc
    19081 cgcaatgggt gcaaaacctg ggcgatgctt ttctggcgca gccgcaggac gtgatggact
    19141 cggtacagcg attgcggcaa ctggcgcaac aaaccggctc gctgaagtca tcaaccgaac
    19201 agaaagttat taccacaacg aagaaagctg taccggtaaa acagacagtc acggcacccg
    19261 tcataccatc caataccgtt ttaactgcca accccgtcat tacagagccg gcaacaaccg
    19321 tcatttccat tgagcccgcc aatcctgatg tggtctatat tcccaactac aacccaaccg
    19381 tggtttacgg gaactgggcc aatactgcgt atccgccggt ttatctgcca ccaccagccg
    19441 gagaaccgtt tgttgacagc tttgtgcgcg gattcggcta tagcatgggc gttgctacca
    19501 cgtacgcact attcagcagc atcgactgag atgacgacga tcatgaccat catcatcatg
    19561 acgatgatga ttatcatcac cacgatggcg gtcatcgtga cagtaatggc tggcaacaca
    19621 acggcgacaa catcaatatc gacgtcaaca atttcaaccg tatcaccggt gagcatctta
    19681 ctgataagaa tatggcatgg cggcacaatc caaactaccg taatggtgtg ccctatcatg
    19741 atcaggatat ggcaaagcgg tttcatcaaa ccgatgtcaa cggcggaatg agtgccacgc
    19801 agctacctgc tccaacacgc gacagccagc gtcaggcggc agcaagtcag tttcagcaac
    19861 gaacacacgc cgcccccgtc attacacgag atacccaacg tcaggcagcg gcacagcggt
    19921 ttaatgaagc tgaacactat gggagctatg acgacttccg cgacttcagc cgtcgccaac
    19981 cactgaccca gcagcaaaag gacgccgctc gtcagcgtta tcagtcggcc tctcctgagc
    20041 agcgccaggc agtccgcgag aaaatgcaga ctaacccgca gatccagcag cgaagggagg
    20101 cggcgcgcca gcgtattcag tccgcctcgc ctgagcagcg ccaggcggtt cgcgagaaaa
    20161 tgcagactaa cccgcagaac cagcagcgaa gagaggcagc gcgtgagcgt attcagtctg
    20221 caactcccga acagcgccag gtgtttaagg aaaaagtaca gcagcgccca ctgaaccaac
    20281 agcaacgtga taacgcccgc cagcgtatac aatcggcatc acctgaacaa cgtcaggttt
    20341 ttcgggagaa agttcaggag agccgcccac aacgtctaaa cgacagtaac catactgtca
    20401 ggctgaataa cgagcaacgg tcagcagtac gcgaacgtct ctctgaacgt ggagcaagaa
    20461 gactggaaag gtaaattgca gacgtaaaaa aagcggcgtg gttagccgct tttttaattg
    20521 ccggatgttc cggcaaacga aaaattactt cttcttcgct ttcgggttcg gcaggtcggt
    20581 aatgctacct tcgaacactt ctgccgccag gcccacagac tcgtgcagag tcgggtgcgc
    20641 gtggatggtc agtgcgatgt cttcagcatc acaacccatt tcgattgcca ggccgatttc
    20701 acccagcagc tcgccgccgt tggtaccaac aatcgcacca ccgatcacac ggtgagattc
    20761 tttgtcgaaa atcagcttgg tcataccgtc tgcgcagtca gaagcgatag cacgaccaga
    20821 agcagcccac gggaaggtgg cggtttcata gctgatgcct ttctctttcg cttctttctc
    20881 agtcagacct acccatgcaa cttctggttc ggtataggcg atggacggga taactttcgg
    20941 atcgaagtag tgtttcttac cggcgataac ttcagcggca acgtgacctt cgtgaacacc
    21001 tttgtgtgcc agcatcggct gaccgacgat atcgccgata gcaaagatgt gcggtacgtt
    21061 ggtacgcagc tgtttgtcaa cgcggatgaa accacggtcg tcaacttcca cgccagcttt
    21121 gcctgcgtcg aggtttttac cgttcggcac acgaccaatc gctaccagca cggcgtcgta
    21181 acgctgcggt tcagctggtg cttttttgcc ttccatcgtc acataaatac cgtcttcttt
    21241 cgcttcaacg gcggtaactt tggtttccag catcaggttg aatttcttgc tgatacgctt
    21301 ggtgaagact ttaacgatgt ctttgtcagc tgccgggata acctggtcga acatttcaac
    21361 cacgtcaatc tgtgaaccca gcgcgtggta aacggtgccc atttccagac cgatgatacc
    21421 gccacccatt accagcaggc gttctggtac ttctttcagt tccagcgcgt cagtggagtc
    21481 ccagatacgc ggatcttcat gcggaataaa cggcagttgg atcgggcgag aacccgctgc
    21541 aatgatcgca ttgtcgaagt tgatcacggt tttgccgttc tcaccttcaa cttccagggt
    21601 gttagccccg gtgaatttac ccagaccgtt gaccactttg actttgcggc ctttcgccat
    21661 accagccaga ccaccggtca gctgattgat cactttctct ttccaggtac gaatcttgtc
    21721 aatatcggtt ttcggttcgc cgaagacgat accgtgttca gccagcgctt tggcttcttc
    21781 gataactttt gctacgtgca gcagtgcttt agaagggata cagccgacgt tcaggcaaac
    21841 accgccaagg gtgttgtaac gttctacgat tacggtttcc agacctaaat cagcgcaacg
    21901 gaaggcagcg gagtaacctg cggggcctgc cccaagtacc acgacctgag ttttgatttc
    21961 agtactcatc atgacctcta tatatttatc tccggcggtc atacccgtcg tctttcaggc
    22021 cgcagcggcg ttagcttcac gccctcaccc cagtcactta cttatgtaag ctcctgggga
    22081 ttctggcgct tgctaccttg ctgcacccag aaatccatag ggtatgtgaa gtaataccct
    22141 aaccaccacc gggtcgttct atccgtcggt attttacaaa attgttaaca atttttaaac
    22201 aacaaacggc aaccgatttg tctattcgct aataacctca atacattcat gagattacca
    22261 gaaaaaagcc ggccgttggg ccggctcttt tacttacatc accagacggc gaatgtcaga
    22321 cagcgtgttg ttaatgatgg taatgaaacg ggcaccatca gcaccgtcga tcacgcggtg
    22381 gtcgaaggag agagaaatcg gcagcatcag acgcggcacg aactctttac cattccacac
    22441 cggctccatc gcggacttgg aaacgccgag gatagccact tccggcgcgt tcacaatcgg
    22501 cgcgaagtgg gtagtaccca ggccgccgat gctggagatg gtgaagcaac cgccctgcat
    22561 ttcgcccgca gtcagcttac cgtcacgcgc tttcttagaa atagtcatca gctcgcgaga
    22621 cagctcgatg atgcctttct tgttgacgtc tttgaatacc ggaacaacca gaccgttcgg
    22681 ggtatccacc gccacaccga tgttgatgta tttcttcagg gtcagacgct gaccgtcttc
    22741 cgacagcgaa ctattgaagc gaggcatctg ctcaagagct gcagcaacgg ctttcatgat
    22801 gaagacaacc ggggtgatct tcacatccag cttacgtttc gccgcttctt cgttctgctg
    22861 tttacggaac gcttccagct cggtgatatc ggttttgtcg aagtgggtaa catgcgggat
    22921 catcacccag ttacggctca ggttagcacc agaaattttc tggatacggc ccagttccac
    22981 ttcttcgatt tcgccaaact tgctgaagtc caccttcggc cacggcagca tgccagggat
    23041 accaccgcca gtcgccgccg gagctgcttc tgcacgtttg atagcttctt tcacgtaagc
    23101 ctgaacgtct tcgcgcagga tacgaccttt acggccagtg cccttcactt tcgccaggtt
    23161 aacgccaaac tcgcgtgcca gacggcggat cagcggggtc gcgtgaacgt aagcgtcgtt
    23221 ttcagcaaat tcagatttgc cttccgcttt cgcagccgga gcttcagctt ttgctgccgg
    23281 agccggcgct gccgcttcct gtttcgcagg agctgccgca ggcgctgcgc cttcaacttc
    23341 gaagatcata atcagcgaac cagttttcac tttatcgcca acgttgactt tcagttcctt
    23401 cacgacgcct gcaaacggag ccggaacttc catagaagct ttatcgcctt ctacggtaat
    23461 cagtgactgt tcagcggcaa ctttgtcgcc cactttcacc atcacttcgg tcacttcaac
    23521 ttcgtcaccg ccgatatccg gaacgttaac ttctttcacg ccagctgctg gtgcaggggt
    23581 cgctgccgga gtcgcttcct gtttagccgc cggagctgcc gcgcctgctt cacccgcgac
    23641 ttcgaagacc ataatcagcg agccggtaga cactttgtca cccacgttca ctttgatctc
    23701 tttcacggta cctgcaaacg gagccggaac ttccatagaa gccttgtcgc cttctacggt
    23761 gatcagcgac tgttcagctt caactttatc gccaactttc accaggattt cggtcacttc
    23821 aacttcgtcg ctgccgatat ccggaacgtt aacgtctttt gccgccgcag ccgcgggtgc
    23881 tgctgccgga gctgcttctt tcttctcttc tgcctgagca ggtgcagcgt ctgctgcacc
    23941 gtcggcggaa tcgaaaatca taatcagtgc gccggtctgg gttttatcgc caacagagac
    24001 tttgatctct ttaacgatac ccgcctgcgg agatggaact tccatagagg ctttgtcgcc
    24061 ttctacggtg atcagcgact gttcggcttc aactttgtcg cccactttga ccaggatctc
    24121 ggtgatttca acttcatcag ccccgatgtc cggtactttg atttcgatag ccattattct
    24181 tttacctctt acgccagacg cgggttaact ttatctgcat cgatgttgaa tttggcgatt
    24241 gcgtcagcaa ccactttctt atcgatttcg ccacgtttag ccagttcgcc cagcgccgca
    24301 accacgacat aagaagcatc aacttcgaag tggtgacgca ggttctcacg gctgtcggaa
    24361 cgaccgaagc catcagtacc cagtacgcga tagtcgtcag ccggtacgta agtacggacc
    24421 tgctcagcga acagtttcat atagtcggta gatgccactg ccggcgcgtc gttcatcacc
    24481 tgagcgatat acggaacgcg cggagtttcc agcggatgca gcatgttcca gcgttcacaa
    24541 tcctgaccat cacgcgccag ctcggtgaag gaggtcacgc tataaacgtc agaacctacg
    24601 ccgtaatctt tcgccaggat ctctgccgct tcacggacgt gacgcaggat ggaaccggag
    24661 cccagcagct gaactttacc tttgctacct tcaatggttt cgagtttgta gataccttta
    24721 cggatacctt cctcagcacc ttccggcatt gccggcatgt ggtagttttc gttcagcgta
    24781 gtgatgtagt agtaaacgtt ctcttgtttt tcaccgtaca tacgctccag accgtcatgc
    24841 ataatgacag caacttcgta agcgtaagcc gggtcgtaag agatacagtt cgggatagtc
    24901 agcgactgaa tgtggctgtg accatcttcg tgctgcagac cttcaccgtt cagggtggta
    24961 cgaccggaag taccgccgat caggaagcca cgcgcttgct ggtctccagc cgcccagcac
    25021 agatcgccga tacgctggaa gccgaacatc gagtaataga tgtagaatgg gatcatcggc
    25081 agattgttcg tgctgtaaga ggtcgccgct gccagccagg aacaccctgc gcccagctcg
    25141 ttgatccctt cctgcagaat ctgacctttc tcgtcttctt tatagtaagc aacctgctcg
    25201 cggtcctgcg gggtgtactg ctgaccgttc gggctgtaaa taccaatctg acggaacaga
    25261 ccttccatac cgaaagtacg cgcttcgtcg gcgatgatcg gtaccagacg atctttgatc
    25321 gacttgttct tcagcatcac gttcagagca cgaacgaaag cgatagtggt agagagctct
    25381 ttgctctgct cttccagcag cgcgccgaag tcttgcaggc tcggcagctc aagcttctcg
    25441 gtgaagttcg gctgacggct tggcagataa ccgtgcagtt tctgacgctg agcgtgcaga
    25501 taggtatgct cttcagaacc ttccgggaag gtgatgtacg gcagtttttc gatatctgca
    25561 tcagacaccg gcacattgaa acggtcgcgg atgtgacgca cgccgtccat gttcattttc
    25621 ttaacctggt gcgcgatgtt tttaccttca gccgcgtcgc ccatgccgta acctttaatg
    25681 gtatgagcaa ggattactgt cgctttgcct ttggtttcct gcgctttctt gaatgcagcg
    25741 tagattttct tcggatcgtg accaccacgg ttcagtgccc agatctgctc gtcagtccag
    25801 tctgcaacca gtgctgcggt ttcaggatat ttaccgaaga agtgttcacg aacgtacgca
    25861 ccatctttcg atttgaaggt ctggtagtcg ccgtcaacgg tttcgttcat cagctggatc
    25921 agtttaccgc tggtatcttt acgcagcagt tcatcccaac ggctacccca catcactttg
    25981 atcacgttcc agccagcacc ttcgaagatg ccttccagtt cgttgatgat cttgccgtta
    26041 ccggtgaccg ggccgtcaag acgctgcagg ttacagttga taacgaaaac caggttatcc
    26101 agtttttcac gggtagcgat ggtgatcgca cctttggatt ccggttcgtc catttcaccg
    26161 tcgccgagga acgcgtaaac ggtctgttta gaggtatctt tcaggccacg gtgttccaga
    26221 tatttcagga atttagcctg gtaaatagca ccaatcggac ccagacccat agatacggtc
    26281 gggaactgcc agaattccgg catcagtttc gggtgcggat aggaagagag gccattgccg
    26341 tgaacttcct gacggaagtt atccagctgc tcctgagtca gacgaccttc caggaaagca
    26401 cgggcgtaaa cgcccgggga gatgtggccc tggaagtaaa ccaggtcgcc gccatcctgc
    26461 tcgttgcgtg cacggaagaa gtggttaaag cacacatcat aaatggttgc ggaagactgg
    26521 aaggacgcca tgtggccgcc cagttcgagg tcttttttcg acgcacgcag caccgtcatg
    26581 atggcgttcc agcggatagc tgaacgaata cggcgttcca gttccagatt acccggatac
    26641 tccggttgtt cttcaacggg gatggtgttg atgtagttgc tgatacctgt gcctgcggct
    26701 acgtttacac cgcctttgcg ggcttcagca agcagttggt cgatcagata ctgagcacgc
    26761 tcaacacctt cttcacggat gaccgattcg atcgcctgga gccagtcgcg agtttcgatc
    26821 ggatccacgt catttgggaa acgttctgac atgggttatt ccttatctat ctaataacgt
    26881 tgagttttct ggaacctgtc ccattgaact ctcgccagaa agctcaataa gacaggttct
    26941 acgtttagtt accgcgcttt tatatgcgct tgatttacaa catcttctgg ataattttta
    27001 ccagaaaaat cactaattct ttcgttgctc cagacgacgc agagaacgct cacggcggct
    27061 ctcttcacgg cttctgtcga gcaaaatttc ttcgataaag gccagatggc gatgcgatgc
    27121 ttcgcgcgct tcttccggct taccgaccat aatcgcttca aatatgcggg tgcggtgact
    27181 actcaccagc ggcagcatct cgcgacgcga atagagcaat tcgaagttct ggcggacatt
    27241 ctgggccaac atcggctcca tacaccttag cagatgaagc agaaccacat tgtgggccgc
    27301 ttcggtgacg gcaatctgat actggagtac ggcgtttgat tccgcgtcca gatcgccaga
    27361 ctgctgcgcc agctctatgg cgtggtggag ttcacggatg cgttccttgt cttcatcggt
    27421 actacgcagc gcggcgtaat aagcggcgat accttccagg gcgtgtcgtg tttcgagcaa
    27481 gtcatactgt gattcaggat ggtcggagag cagctccacc agcggatcgc tgaagctttg
    27541 ccataggctg ctctggacaa aagtgccgcc accctggcga cgaagcaaca agcccttcgc
    27601 ttcgagacgt tgaatcgcct cacgcaagga gggacgggag acgtcgaact gttttgccag
    27661 ttcgcgttcc ggtgggagtt tttcgcccgg gcggagagtg ccttcgagga tcaaaaactc
    27721 cagttgctgc tcaatcacat cggagagttt tggttggcgg attttgctgt aggccatgag
    27781 ttcctgtctt aagccacttg ccgaagtcaa ttggtcttac caatttcatg tctgtgacgc
    27841 taaagtaaca aagtattcac cttatgtcca tacaggtttt gattgaaatc atgaaactgt
    27901 gcacatttta acaacttgac atatataacg tttcaaagtt gtaactatgc acaaatgtag
    27961 actttacgta ggaaagggat tttttaacga ttattaaact ataaaaatcc gaattgaacc
    28021 gattcactta ccaatttttt gatttttaag tcaataaaaa cgaatttaaa ttcactctgc
    28081 atattgagag gggttgaggc tgagctttac aaacggtttc tttttaagca actcatcttc
    28141 aaccatgcat aaagcgggtg cattcgctgc cgcataccat tattcttgat ctgacggaag
    28201 tctttttgta acaattcaaa cctccttgat gtaaacaaat caatacaaca aacggaattg
    28261 caaacttaca cacgcatcac tgcgtagatc aaaaaaacaa tcaccgcacg aggtttcatg
    28321 atggaaggtc aacagcacgg cgagcagcta aagcgcggcc ttaaaaaccg ccatattcag
    28381 cttatcgcgc tgggtggcgc gatagggaca gggctattcc taggtagcgc ctccgtaata
    28441 cagtccgcag ggccagggat tatcctgggt tacgccattg ctggttttat cgcctttctg
    28501 atcatgcgtc agctgggtga aatggtggtc gaagaacctg tcgcaggctc ctttagccac
    28561 tttgcttata aatactgggg cagttttgcc ggtttcgcct ctggctggaa ctactgggta
    28621 ctgtacgttt tagttgccat ggcagagctg actgccgtgg gtaaatacat tcagttctgg
    28681 tatccggaaa tcccaacctg ggtttctgcc gccgtattct ttgtggtgat taacgccatc
    28741 aacctgacca acgttaaagt gtttggcgag atggagttct ggtttgccat tatcaaagtt
    28801 attgcggtag tagcgatgat catcttcggc ggctggctgc tgttcagtgg caacggcggc
    28861 ccgcaggcga gcgttagcaa cctgtgggat caaggtggtt tcctgccgca cggcttcacc
    28921 ggactggtga tgatgatggc gattatcatg ttctcgttcg gtggtctgga actggtgggg
    28981 atcaccgcag cagaagctga taacccggag caaagtatcc cgaaagcaac taaccaggtt
    29041 atctaccgca tcctgatttt ctatattggt tcgttagccg ttctgctctc actgatgccg
    29101 tggacccgcg ttaccgccga taccagtccg tttgtgctga tcttccacga gttaggcgat
    29161 acctttgtgg cgaatgcgct gaacatcgtg gtactgactg cggcgctctc cgtgtacaac
    29221 agctgcgtat attgcaacag ccgtatgctg tttggtctgg cacaacaggg gaacgcgcca
    29281 aaagcgctgg cgtctgtcga taaacgcggc gtaccggtta acaccattct ggtgtctgcg
    29341 ctggttacag cattgtgcgt attgattaac tatcttgctc cggaatccgc atttggcctg
    29401 ttaatggcac tggtggtatc cgcactggtg atcaactggg cgatgatcag tctggcgcat
    29461 atgaagttcc gtcgcgccaa gcaggaacaa ggcgtggtaa ctcgcttccc tgctctgctt
    29521 tatccgctgg gtaactggat ctgcctgctg tttatggcgg cggtactggt gattatgctg
    29581 atgaccccag gaatggcgat ttcggtatac ctgatcccgg tatggctgat cgtgttaggt
    29641 atcggctatc tgtttaaaga gaaaaccgcc aaagccgtaa aagcgcatta atctctctac
    29701 gccctcaccc gtacagggtg agggcaataa tctctacacc aacgccccgt aaatcgtcag
    29761 tagcgcaatc accaccacga ccacgaacga ggttttcttc gccattgaaa ccgctgcctt
    29821 cggcgtctcc accttatcga catgcggttc acgcgccaga gagaactgcg ccagacgcgt
    29881 taatacctga tattgcgaag tatggaaatc acccagcgaa gcaaaccagg ccggtaacgc
    29941 tttctcacca tgaccgatca aggcatatac cacacccgca agacgaaccg gcacccaatc
    30001 cagtacatga agcactgcat caatgccgga ctgtaaacga tgatgcggcg tctggtatcg
    30061 cgccagccag tattgccatg cacgcaagaa cgcatacccc atcagcgtaa cgggtcccca
    30121 ggttcccccc acaatcagcc agaacagcgg tgcaagataa aaacgaaagt taatccacag
    30181 caatgcattt tgcagctcac gcaaatactc acgttcgtcg cagcctgccg ggacgccgtg
    30241 aatcatggtg agttcgccag ccatcgtggc acgggcatgg ctatcattac gtgaagcagc
    30301 tgtcagataa gcatgataat gaagacgaac tttacctgcg ccaatacaca gcaaaccaat
    30361 cagcagccac accagcagcg tgggaacgtt gaacaatact ccctgcaatg cgcgtaacag
    30421 taaaaaagtc acacccatcg caataatggt catgcctaac gtgcgcccga gagaaaaatg
    30481 tttcacccgc cgaaagaacg cttcaagacg atgatcaagc tgccagtgct cgcccaactt
    30541 aaacaggcgc tcgaaaatta acaccagtaa ggttgtaaat agcgtcatgt tgtctccttg
    30601 ctgaccagcg cacgaaaccg tgcccaatca aatgcaggac cgggatcggt tttccgatcc
    30661 ggcgcaatat cacaatggcc cgtcatgtta ttagcgatat cgggatagcg atctatcagt
    30721 gcccgcgtaa ccgccgcaag ctgttgatac tgcgcatcgg tatacgccag agtatcggtg
    30781 ccttcaagct caatcccaat agaaaaatca ttgcagcgtt cacgcccctg atactgagag
    30841 actcccgcat gccaggcacg tttatcgaaa ggaacatact ggactatttc accatcacgg
    30901 cgaatcaaac agtgagcgga gacgcgcaaa tgggcgatct cagcaaagaa aggatgtgcc
    30961 tgcggatcaa tagttccagt gaataatgcg tcgatccacg gaccgccaaa ctcgcctggc
    31021 ggcaggctaa tattgtgcac caccagcagg gtgggtgttt cgtcatccag gcggcaatcg
    31081 taatgtggtg agggaacgcg gcgcgcgcca accagccacc cctgttctaa caacatgcag
    31141 gatctcctta tatgtggtgc taatacccgg ttcagagtag catgtttcta ccttatgatt
    31201 cgttagctat ctggagtttt aacatgccgc ctcgccgcta caaccctgac acccgacgtg
    31261 acgagctgct ggaacgcatt aatctcgata tccccggcgc ggtggcccag gcgctgcggg
    31321 aagatttagg cggaacagtc gatgccaaca atgacattac ggcaaaactt ttaccggaaa
    31381 attctcgctc tcatgccacg gtgatcaccc gcgagaatgg cgtattttgt ggcaaacgct
    31441 gggttgaaga ggtgtttatt caactggcag gcgacgatgt caccataatc tggcatgtgg
    31501 atgacggcga tgtcatcaac gccaatcaac ccttgttcga acttgaaggc ccatcccgcg
    31561 tgctgttaac gggcgaacgc actgcgctta attttgtgca aaccctttca ggagttgcca
    31621 gtaaggtacg ccactatgtc gaattactgg aaggcaccaa cacgcagttg ttggatacgc
    31681 gcaaaacctt acctggcctg cgttcagctc tgaaatacgc ggtactttgc ggcggcggag
    31741 cgaatcaccg tctggggctt tctgatgcct tcctgatcaa agaaaaccat attattgtct
    31801 ccggctcagt gcgccaggcg gtcgaaaaag cgtcctggct gcatccggat gcgccagtag
    31861 aagtcgaagt agagaatctg gaagaacttg atgaggccct gaaagccgga gccgatatca
    31921 tcatgctgga taacttcgaa acagaacaga tgcgcgaagc cgtcaaacgc accaacggca
    31981 aggcgctact ggaagtgtct ggcaacgtca ctgacaaaac actgcgcgaa tttgccgaaa
    32041 cgggcgtgga ctttatctcc gtcggtgcgc taactaaaca cgtacaagca ctcgaccttt
    32101 caatgcgttt tcgctaattg ctgacagatt tatccgctcc atcgcgggcg gataatctcc
    32161 ccttccccaa ctttcttcgt aacgcctcgc aaatttcttg tctttcgctg caacgaaata
    32221 aaaaacctcg gaatctgctt tcccgtttcg ttttttgccc ctcgcctgtg ctgatggcgt
    32281 ttgccaaagt agcaccaacc aaatcaagga gcgaaacaga tggacaagca acgcggtttt
    32341 acacttatcg aactgatggt ggttattggc atcattgcca ttttaagctc cattggtatt
    32401 cccgcttatc aaaactacct gcgcaaagcc gcactcaccg acatgctaca aacctttgtg
    32461 ccttaccgta ccgccgtaga gttgtgcgcg ctggaacatg gtggattaga tacctgcgac
    32521 ggtggcagca atggcattcc ctcgcctacc accacccgct atgtttcagc catgagtgtg
    32581 gcaaagggcg tggtgtcgct gaccgggcaa gaaagtctca atgggctaag cgtcgtcatg
    32641 acgccaggtt gggataacgc aaacggcgtc accggctggg cgcgcaactg caatattcaa
    32701 agtgacagcg cattgcagca agcctgcgaa gatgtcttcc gctttgatga cgccaactaa
    32761 ggagcggcaa tgaatattcc acagctcacg gccctgtgcc tgcgttatca gggagtcttg
    32821 ctggatgcca gcgaagaagt ggttcatgtt gcggtggtcg atgccccctc acatgagttg
    32881 ctggacgcat tgcatttcgc taccaccaaa cgtattgaga tcacctgctg gacgcgccaa
    32941 caaatggaag gtcacgccag tcgcacacaa cagacattac ccgtagctgt tcaggagaag
    33001 catcagccca aagcagagtt gctgactcga acgttacaat ctgcgctgga acaacgcgcg
    33061 tctgatattc atatcgaacc agcggacaat gcctaccgca tccgcttgcg tatcgacggc
    33121 gtattgcatc ctttaccgga tgtttcaccg gatgccggag tcgcattaac agccagatta
    33181 aaagtgctgg gaaacctgga tattgcggaa catcgcctgc cgcaggacgg gcaattcact
    33241 gtcgaactgg caggaaacac cgtctcattt cgtattgcga ccttaccatg tcggggtggt
    33301 gaaaaggtgg tattaaggtt gttacagcag gtgagccagg cactggatgt taacacgctg
    33361 ggaatgcagc cgttacaact ggcggacttt gctcatgcct tgcaacaacc acagggactg
    33421 gtgctggtaa ctggccctac aggcagtggc aaaacggtca cctggtgttg tccacgctac
    33481 acaccaattc caccactgaa acgctggtac gtttacagca aatgggggtc gcccgctgga
    33541 tgctatcatc ggcgcttacg ctggtaatag cccagcgtct ggtacgtaaa ctttgcccac
    33601 attgtcgccg acagcaaggg gagcccatcc atattccaga cactgtatgg ccgtcgccgc
    33661 tgccccactg gcaggcaccc ggttgtgtac attgctacca cggtttttat ggtcgtaccg
    33721 ccttgtttga agtcctgccc attacaccag ccatacgcca gcttatttcc gctaatactg
    33781 aagttgaatc gctggaaacg cacgcccgac aggcgggtat gcgtacgctt tttgaaaacg
    33841 gctgcctggc cgtggagcaa ggcttaacca cctttgaaga gttaatccgc gtactgggga
    33901 tgccgcatgg caagtaagca actctggcgc tggcatggca taaccggcga cggcaatgcg
    33961 caagatggga tgctatgggc agagagccgt actttactgc ttatggcact acagcaacag
    34021 atggttaccc cactaagcct gaagcgaatc gccatcaatt ctgcgcagtg gcgaggagat
    34081 aaaagcgcgg aagtcattca tcaactggcg acgctactta aagccgggtt aacgctttct
    34141 gaagggctgg ctctgctggc ggaacagcat cccagtaagc aatggcaagc gttgctgcaa
    34201 tcgctggcgc acgatctcga acagggcatt gctttttcca atgccttatt accctggtca
    34261 gaggcatttc cgccgctcta tcaggcgatg atccgcacgg gtgaactgac cggtaagctg
    34321 gatgaatgct gctttgaact ggcgcgtcag caaaaatccc agcgtcagtt gaccgacaaa
    34381 gtgaaatcag cgttgcgtta tcccatcatc attttagcga tggcaatcat ggtggttgtg
    34441 gcaatgctgc attttgttct gccggagttt gccgctatct ataagacctt caacacccca
    34501 ctaccggcac taacgcaggg gatcatgacg ctggcagact ttagtggcga atggggctgg
    34561 ctgctggtgt tgttcggctt tctgctggcg atagccaata agttgctgat gcgccgcccg
    34621 acctggctta tcgcgcggca gaaattgctg ttacgcatcc cgattatggg ttcactgatg
    34681 aggggacaaa aactcacgca gatctttacg attctggcgc tgacacaaag tgcaggcatt
    34741 acttttttac agggcgtaga gagcgtcaga gaaacaatgc gctgcccgta ctgggtgcaa
    34801 cttctgacac aaatccagca cgatatcagt aacggtcatc ccatctggct ggcgctaaaa
    34861 aatgccggtg agtttagccc gctctgtttg caattagtga gaacaggaga ggcatccggc
    34921 tcgctggacc tcatgttaga caacctcgcc catcatcatc gggataacac aatggcgctg
    34981 gcggataacc tcgcagcctt actggaaccg gcgttgctga tcataacggg aggaattatc
    35041 ggtacgctgg tggtggcaat gtatctgcca attttccatt taggcgatgc gatgagtggg
    35101 atgggataac gctggcgtgt tgctccacgc cagcgttggg agattacagg ttgttgaaga
    35161 tgcggttttc ttgttccagc acacgaataa acgtggtgcg cttggtcagc tctttcaggc
    35221 gtgaagcccc aacgtatgta caagctgaac gcaggccgcc caaaatatca cgcgcggtat
    35281 tttcaacctg gcctcgcagc ggcagcttaa cggttttacc ttctgctgcg cggtattccg
    35341 caacgccgcc aacgtgacgt ttcatcgcag actcggagct catgccgtag aacagcataa
    35401 atttctcgcc gttctcctca acgatgcgac cgccgctctc ttcgtggccc gccagcatgc
    35461 cgccaagcat gacgaaatcg gcaccgccgc caaaggcttt cgccacatcg cccggcgtgg
    35521 tacagccacc gtcgctgacg atcattccgc ccagaccgtg cgccgcatcg gcacattcga
    35581 ttaccgcaga aagctgcgga taaccgacgc ctgttttgac gcgagttgta caaacagaac
    35641 ctgggccaat gccaacttta acgatatcag caccggagag gataagctcc tcacacattt
    35701 caccagtcac tacgttgcca gcacaaatgg ttttggtcgg ccacgcttca cgcgctttcg
    35761 caacgaactg cacgaagtgt tcggaataac cattcgccac atcaatacaa acgaagttta
    35821 atgccgggtt caggtcgaga atctgtttag ttttttcgaa atccgcatca gacgtgccgg
    35881 tagaaaccat cacatgtttc agcacatcag cggaagaatt gttgataaac gcttgccact
    35941 cttcgacaga aaagtgttta tgcacagcag tcagaatatc aaaagaagcc agcgcagagg
    36001 ccatagaaaa tgtgcctacg gtgtccatat ttgcggcgat aatcggcacg ccggaccagc
    36061 tctgacctga atgtttgaag gtgaattgac gttccagttc aacatcggaa cggcttttaa
    36121 gagtggagcg tttagggcgg atgagaacgt ctttaaaacc taacttcaga tcttcttcaa
    36181 tacgcatgtg cggattcctg gggttaatgg ctataagggt aagagcgcaa ctccagtgac
    36241 gctatcatac gcactaatca atgcggcgca agactgcgaa attgtctttt tttacgctac
    36301 aatcccataa atttacctgg taaatagtga gcgaacgttt catcaatttc actttcgcgc
    36361 gctcattttt aacgttttga taatcatgat gaattcccgg gaatataaat tatggggtat
    36421 atagttgcct taacgggagg cattggcagt ggtaagagta ccgttgccaa tgcgtttgct
    36481 gatctcggaa ttaacgtcat tgatgccgat attattgcgc gtcaggtggt tgaaccaggt
    36541 gcacctgcgc tacatgccat tgctgatcac tttggcgaaa acatgattgc tgctgatgga
    36601 acattgcagc gccgggcctt gcgcgagcgg atcttcgcca acccggaaga gaaaaactgg
    36661 cttaacgacc tgctgcatcc gctgattcaa caagagacgc aacaccagat ccagcaagca
    36721 acttccccct atgtactgtg ggttgtgcca ttgctggtag aaaactcact gtataaaaaa
    36781 gcgaatcgag tgctggtggt ggatgtcagc ccagaaacgc aacttaagcg caccatgcag
    36841 cgcgatgatg taactcgcga acatgtcgaa caaatccttg ctgctcaggc aacgcgcgaa
    36901 gcccgccttg ccgtggcaga tgacgtcatt gataataacg gcgcaccgga tgctatcgca
    36961 tcggatgttg cccgcctgca cgcacactat ttgcagcttg cgtcgcagtt tgtctcacag
    37021 gaaaaaccgt aatgcagacc caggtccttt ttgaacatcc actaaatgaa aaaatgcgta
    37081 catggctgcg cattgagttt ttgattcagc aactcaccgt taatttaccc atcgttgacc
    37141 acgctggcgc gctgcatttc ttccgtaatg tcagtgaatt actggatgtt ttcgagcgcg
    37201 gcgaagtccg cactgagctg ttgaaagaac ttaaccggca gcaacgtaaa ctccagacct
    37261 ggattggcgt gcctggcgtg gaccagagcc gtattgaagc attaattcag cagttaaaag
    37321 cggcggtgag cgtattaatt tccgcgccgc gtatcgggca atttctgcgt gaagatcgtt
    37381 tgattgctct ggtgcgtcag cgactgagca tcccaggcgg ctgttgcagc tttgatttac
    37441 ctacattgca catttggctg catctacccc aggcgcagcg cgacagccag gtagaaacct
    37501 ggattgccag cctgaacccg ctcacccagg cacttaccat ggtgctggat ttaattcgcc
    37561 agtcggcccc cttccgtaaa caaaccagcc tgaatggttt ttatcaggat aacggtggcg
    37621 atgccgactt gctgcgcctg aatctgtcgc tcgattcaca gctttatccg caaatttccg
    37681 gacataagag ccgttttgcc attcgtttta tgccgctgga cactgaaaac ggacaggtac
    37741 cggaacgtct ggatttcgaa ctggcctgtt gctaaggagt aaaaatgtca gaaactatta
    37801 cggtgaattg cccaacctgc gggaaaacgg tggtgtgggg tgaaatcagc ccgtttcggc
    37861 cattttgctc caaacgttgt cagctgatcg acctcggaga atgggctgct gaagaaaaac
    37921 gaatccccag cagtggcgat ctttccgaaa gcgatgactg gagcgaagag ccaaagcagt
    37981 gacatttgtc tgatgcctca cgtaggcctg ataagacgcg gacagcgtcg catcaggcat
    38041 cttgtgcaaa tgtcggatgc ggcgtgaacg ccttatccga cctacagacg cttaagcttc
    38101 gcaattaccg gttcattggc tggcggaaaa tcatcggcat taagaccgac cagcgacatc
    38161 cactcaccgg gttgcccttc tttaccccac ggcccccctt cccaactttc gaccagccag
    38221 aaccacagag ttatatgcct gtccgggaat tcatattcca gtttttcaaa tagcgaaaaa
    38281 tgttgtgggg taatcccgac ctcttcctga agttcacgca ccaccgcctg ttccggcgtt
    38341 tcgcccattt caattttacc gccgggaaac tccagtttat tcgccatgtg cgcatctgct
    38401 gcgcgacgtg ttataaagat ttcattgttc tcgttgcgaa taatacctac cgcaatttgc
    38461 agctttttca ttgtcttaaa cctataaaaa aggcgcagaa tcctgcgcct tttacttcaa
    38521 ctgttagctt ttattgcagg cgaccatggc actgcttgta ttttttacca gaaccgcacg
    38581 ggcaaggatc gttacgtcct actttgcgtt caccggtttg cgccgccagt gcagctgcgg
    38641 ctgcagagtc gtcatcctga tggctaagct gctgcatttg tgctaaacgc tcggcttcca
    38701 tacgacgctg ttgttccagc tcctcaacct cttcaggcat acgtacctga actttgctca
    38761 gcgtactgat aacttcatat ttcaacgact ccagcatcgc tgcaaacatg gagaacgatt
    38821 cacgtttgta ttcctgcttc ggatctttct gtgcatagcc acgcaggtgg ataccctgac
    38881 gcagatagtc catcgctgcc aggtgctctt tccacaggga gtcaagcgtt tgcagcatga
    38941 cgcctttctc gaagtgacgc atcatctcag caccaaccac ttcttcttta cgctgataca
    39001 cttcgatgga ctgcgccaga atgcgctcac gcagcgtctc ttcatgcagt tctggttctt
    39061 tatccagcca ctcggtaatt ggtaaatcga ggtcgaaatc gttcttcaga cgttcctgca
    39121 gccccggaat atcccacatt tcttccagcg actgtggcgg aatgtaggca tcaatggtgg
    39181 ctttgaacac atcttcacga atgctgttga tggtttcgct cacatcgctg acatccagca
    39241 gttcgttacg ctgggagtaa atggcgcgac gctgatcgtt agccacgtca tcatattcca
    39301 gcagttgctt acgaatgtcg aagttacgac tttcaacttt acgctgggcg ttggcaatcg
    39361 ctttagtcac ccacgggtgt tcaatggctt cgcctggctt catacccagt ttacgcatca
    39421 tgccggatac tcggtcggaa gcaaaaatac gcatcagcgc atcttccatc gacaggtaga
    39481 aacgggaaga accagcatcc ccctgacgac cagaacgacc gcgcaactgg ttatcgatac
    39541 gacgggattc gtgacgctcg gtaccgatga tatgcaggcc acctgcttcc agtaccgcat
    39601 cgtgacgtac ctgccagtcg gctttaattt tttcaatttg ctctgcggtc ggattttcca
    39661 gcgcggcaac ttctgcctgc cagctacctc cgagcacaat atctgtacca cgacccgcca
    39721 tgttggtcgc gatagtcacc gcagccggat aacctgcctg agcaacaatc gccgcttcgt
    39781 tggcgtggaa tttggcgttc aggacattgt gcttaatacc ggctttggtc agttcgtttg
    39841 acaccagctc cgatttttcg atggagatag tacccaccag caccggctgg cctttcgcag
    39901 tacgttcttt gatatcttca atgatcgcct gaattttttc cgcttcagtc atgtagacca
    39961 ggtccggcag atctttacga atcattggac ggttggtcgg aacaacgacg gtatccagtt
    40021 tgtagatgga gctaaattcg aaagcttcgg tatcagcagt accggtcatc cccgccagtt
    40081 tttcatacag acggaagtag ttctggaagg tgatcgaagc cagcgtttgg ttttcgttct
    40141 ggatctgcac accttctttc gcttccacag cctggtgcag accatcggac cagcgtcggc
    40201 cctgcatggt acgaccggtg tgttcgtcaa cgatgataac ttcaccatct ttaacgatgt
    40261 agtcgacgtc acgggtaaac agcgcatgag cgcgcagcgc cgccgttacg tggtgcatca
    40321 gcatgatgtt ggccggagag tacagagact ccccttcatc catgatgccc tctttcacca
    40381 gcagttcttc aatcagcacc agaccacgtt cggtcaggtt cacctggcga gatttttcgt
    40441 ccaccgagaa gtggccttcg ccctggaagg tttcggagtc ttctttttcc tgacggatca
    40501 ggtgcggaat aattttattc acgcgtttat acatttccga gctgtcttct gccgggccgg
    40561 aaatgatcag cggtgtacgc gcttcatcga tcaggatgga gtccacttcg tccaccagcg
    40621 catagtgcag tttacgctgt acacgttctt cagggctgaa cgccatgttg tcgcgcaggt
    40681 agtcaaagcc gtattcgttg ttcgtaccgt aagtgatgtc agctgcgtaa gcttcgcgct
    40741 ttgccggtgc tggcatgccc ggcaggttga taccaacagt caggccaagg aattcaaaca
    40801 gcggacggtt gttttcggcg tcacgttgcg ccaggtagtc gttgacggta actacgtgca
    40861 cgcctttacc ggttagtgcg ttcaggtaag caggcagcgt tgcggtcagg gttttacctt
    40921 caccggtacg catttcggcg atgcagcgtt cgttaagaac cataccgccg agtaactgaa
    40981 cgtcgaagtg acgcatacca aagacgcgct tacttgcctc acgcaccacg gcgaaagctt
    41041 ccgggatcag attttccagc acttcgcctt tttccagacg cgcacgaaac tctgcggttt
    41101 tccctttcag ttcttcatcg gagagttttt ccatctccgg ttccatggca ttgatgatgt
    41161 tgaccacttt gcgcatccgg cgcagggtgc gatcgttacg actaccgaaa actttagtta
    41221 acaatttgat tagcataata aaatctcaaa cgccccgcgt tacggagtta ataaaatgaa
    41281 gtaaaggttt attgttgtta ggtgaggcgt tgagggccag cacggatgcc ttgcgcctgg
    41341 cttatccaga cgggcgtgct gaattttgct tgtggggtaa aatgcgcata atcaatgcga
    41401 taaccctttt cagacggcgt gccttcctgg gtcagcagcg cgctgagcgt atccagtaat
    41461 gcaagatgtt gcgcctgaag aggcaaagat tcttcagcaa cgggcagtgt ttgcggtgcc
    41521 attgcgaaag aaagatgacg gattaccgta cgaatagcat gttgatgcca gtaatcaacg
    41581 gaatagttcg aattcgggcg gcgcgtgttc gcttccagca aggccaattg accaaagtta
    41641 actttggctg aaggctcgtg gttgcgggtt gtcgcttttg cgggcgcgtt tggttcggcg
    41701 gcgttgctga gcgcaggcaa acctaaactc gccgcgacca tccctaataa gagatgcggc
    41761 cagaagtagc gtttaccaaa ctgtcgccag cgcgtcagta ttccactcac gttattgcca
    41821 tcccgatcga attcaaacgt atttagcgca caaatcttat cattaaagcc tgaagtccac
    41881 agcacgaatg aagataagtt gtgcgtaaaa gtgcttaaga aaacagcgtt cgcaccgttt
    41941 tttatagctc ttataaaatt caaaaaaaaa cgactggttc acctggccgg agagagtgcc
    42001 agatttgcca gtcgaatttt atacgacagt ataaatgtcg ttatgccagt acagctgaag
    42061 gcgctttgaa ggccaacggc agttctgcgt cgtcctggaa ggtcacatat tcccaggctt
    42121 cctgtttcgc caggacagcc tgcagcagtt tgttattcag tgcatgaccg gatttataag
    42181 cggtaaatgc accaataata ttgtgaccac acatgaacaa gtcaccgatc gcatcgagca
    42241 ttttgtgacg cacaaattcg tcttcaaaac gcaggccgtc ttcgttcagt acgcgataat
    42301 cgtcaacaac gatggcacaa tcgaagctgc cgcccaggca caaaccacgg gactgcagat
    42361 attcgatatc acgcatgaaa ccgaacgtac gcgcacggct gatctggcgc ataaacgcat
    42421 cagcggagaa gttcatcgca tagcgctggt tgctggaatc aatagccgga tggttaaaat
    42481 cgatggtgaa atccagcgaa aaaccattgt acggcttaaa ttcagcccac ttatcgccat
    42541 cttcgacacg aacagtctct ttgatgcgaa caaatttttt ggcgcagttc aactcgtcga
    42601 taccggcgtc aagcagcagg tatacaaacg gagcggcgct gccgtccatg atcgggattt
    42661 ccggcgcgtt aacttcgata acaatgttat cgatgcccag gcccgcgaga gcagcattga
    42721 ggtgctctac ggttgaaatc cgtacatcat gctcgttgac cagacacgta cagagcatgg
    42781 tatcacgcac agatttggca tcggccggga aatctaccgg tggattcaag tcggtgcgac
    42841 gatagatgac cccggtgttg gccggcgcag ggcgtaacgt cagggtgact ttcttgccgg
    42901 tatgtaaacc gacacccgtc gcctgaacga tacgtttaag tgtcctttgt ttgatcatcg
    42961 tattatctcg ccaaattacc tatccaaccg aagtgtacta tacattcggc gggccagttt
    43021 agcacaaaga gcctcgaaac ccaaattcca gtcaattctt aatcagcttg cttacgtagg
    43081 aatgctggga tatccagata atccggctct ttcgcagttt gcggcgcatt gtcattcact
    43141 actttagcaa ccggcttctg ctcctgggtc agcggagcca tcccatgctg ctggtagcga
    43201 tccatcactg gctgctgaac ctgcttattg gtcaccagag tgatttcagg acgtttgtcc
    43261 atgccgatac ctgtcgcaac aacggttacg cgcagctcgt cattcatatc cgggtcaaga
    43321 gaagtaccga taaccacagt cgcgttgtcg gaagcaaatg cacggatggt gttacctacc
    43381 gtttcgaact catccagacg caggtcgaag cccgccgtga tgttaaccag cacgccgcgc
    43441 gcgccagaca ggtcgatatc ttccagcagc ggagaagaga tagccatttc agcagcttct
    43501 tccgcacggt cttcaccgct cgccacgcca gaacccatca ttgcgtagcc catttcagac
    43561 attacggtgc gtacgtctgc aaagtccacg ttcatcaaac ccggacgagt aatcagttcg
    43621 gcgatacctt gcacagcgcc tttcagtaca tcgttcgctg cgccaaacgc atccagcagg
    43681 gagataccgc ggcccagaac tttcagcagt ttgtcgttcg ggatagtgat cagagagtcc
    43741 acatgcttgg acagttcagt gatcccctgc tcggcgaatg ccatacgctt cttgccttca
    43801 aagttgaaag gcttagtgac gacagcaacg gtcaggatac ccaaatcttt tgccacttca
    43861 gcgacgactg gtgctgcacc tgtaccggta ccaccaccca tacccgcagc aataaagacc
    43921 atgtctgcac cttccagcgc cgcacgcaat gcatcgcgat cctcatcagc cgcattgcgg
    43981 ccaacttctg gattagcgcc agcgcccagt cctttggtga taccgctacc gatttgaatc
    44041 gtctgtccaa ccgctgtttt acgcagcgct tgtgcatcgg tatttaccgc gaagaattca
    44101 acaccttcaa tacgctcgcg caccatgtgt tcaacagcat taccgccgcc gccgccgacg
    44161 ccgatgactt taatcaccgc gtcattggta agttccattg gttcaaacat agtttctctc
    44221 cgatttgtgc ctgtcgcctg agtccgtaat catcgtcggc ctcataaaaa ttaaaactct
    44281 tttcgcagcc aactattgag tcgcttgatc cacgagccaa ctgatgctgt aacacgtttt
    44341 tctacttcag cttcaccgtt aagatgtgac tctttcccat agtgaagcaa tcccaccgcc
    44401 gtcgaataat acggctcctg agcataatcc gttaaaccgg taatgttcag cggcgcgccg
    44461 atacgcactt gcgtatgaaa cacgcgctga gcacaggctg caagaccttc gatctgcgct
    44521 gcgccaccgg ttaatacaat gcctgccgcc aggtgatgtt taaccccttg ttggcgaagc
    44581 ttttcctgca actgcaatat ctcttcgttg accaggttga gcaactcggt atagcgcggc
    44641 tcgatcacct ctgccagggt ctgacgttgc agactccgcg gcggacgacc gcctacgctc
    44701 ggcacttcca cgctctcatc ttttccaacg atggaaccca gcgcacaacc gtggcgaact
    44761 ttaatcgctt cggcgtcgct tggcggcgtg ccaaaggcgt aagcgatatc actggtcacg
    44821 acattgccag cataaggaat tacctttgtg tggcgcaatg ccccaccggt ataaacggcg
    44881 atatccattg taccaccacc gatatcgacg acgcagacac ccagttcacg ttcatcttcc
    44941 gtcaataccg aataacttga tgccagtccg gcaaatatca gttggtcaac tttcagccca
    45001 caacgttcaa ccgctttgac gatgtttttc gccatatcgt tgtgacatgt gatcaggtgc
    45061 acttttgcct gcatccgcac acccgaaagt cctaccggat tcttgatccc ttcctgatag
    45121 tcaatcgcat actcttgcgg gatcacatgc agcacacgat gctcatcgcg cacacgcacc
    45181 gatttcgcgg tatggacgac gttttccaca tcttcttgcg tcacttcttc ttcagaaata
    45241 ggcaccatac caatttcatt ctggcagctg atgtgcttac cagaaagcgc cagatatacc
    45301 gaagagatct gacaatctgc catcaattct gcctggtcaa tggcgcgttg tacgcacttg
    45361 accacggatt cgaggtcgtt caccccgcct ttatccatac cacgtgacgg gcagctgccc
    45421 acgccaatga tattgaccat accgtcgggc agaacttccc ctactaaagc ggcaaccttc
    45481 gcggtaccaa tttccagtcc tactaccagt tttctgtccg tcgccttgat cattgttgtt
    45541 ctgcctgtgc ctgattttgt tgctgagtag attcctctgg cggcaagggc gcccagccta
    45601 ctgccgctcc agagtcataa cgcaaatcaa cgtagctaat ccgtttgcca tcggtttgcg
    45661 cctgctgctg taaaaccgga taaagttcta caaagcgaac caaacgtttc atcgtatcgc
    45721 cccggccaag attgagctta atatcgttat tcagcgtcaa ctgccaggaa cgccgcgcgg
    45781 tcatcgccgc ttccttcaga gtaaatctgt cctttgccag catctgcccc atttcgcgat
    45841 agccctgcaa cacttcatta gcgctgcctt ccgggccata cagcattgga agcacctgct
    45901 tgctggtgcg atctggcggc acgctgaagg tgtttccttc cgcgtctacc atgtgttgat
    45961 cattccaccg cgcaatcggc acatattcaa ccagatgaat cttcaattca tcaggccact
    46021 gctttctgac gctcacctgc ttaatccacg gcaggcgttg ttctatttgc gtctggatga
    46081 tgttgacatc ctgggtcata aaggtacccg gctcacccaa tgccaggatc gactgccgga
    46141 tatcgtcatt acgcgtgtaa tggcgttcac cggtcaacac cagctttgag agcggcaggc
    46201 gttgcgcatc ttccatccag ctcaacacga cccagccgct caccaacact gtcgttaaaa
    46261 cggtcagcag gaaaaggatc cccgccagac gcgttccatt attgcggcga gaagaaacct
    46321 cttcttcgct gtttcgcgtg ttcagagcag cctgcgacat attagtccgc cagttccaga
    46381 attcgtacta ccaactgcga gaagctcata cctgcctgac gtgccgccat cggcaccagg
    46441 ctgtggctgg tcatacccgg tgaggtattg gcttccagca gataaaactg tccatcactg
    46501 tccagcataa cgtcaatacg tccccatcct ttgcaaccta acgtcgtcca tgctttcagc
    46561 actaatgcct gcaaattggc ctcttgtgac gcttccagac ctgcggggca gaaatactgt
    46621 gtctcatcag agagatactt cgcctcataa tcatagaagg ttccggacgg ttgaatacgt
    46681 attgacggta aaatttcttc accgagtatc gcaaccgtga actccggccc acttagccat
    46741 ttttcaatca atacttcttc atcgtgctga aatgccaatc ttaatgcatc ttgtagagca
    46801 ttttctgcta ctacttttga cattcccaca ctggaacctt cgcggctcgg cttaacgata
    46861 accggcaaac ccagagcaga aatttctgct aactgcttat cgctcaggcc tttttcaaac
    46921 tctgcgcggg ttaacgctac ccacggcgcg accggtaaac cggcaccttg ccacagaagt
    46981 ttgctgcgta gtttatctat tgaaagcgca gatgccatca ctccgctgcc ggtataaggc
    47041 aagcccatca gctcgagcat gccctgcagc gtaccatctt caccgccgcg accgtgtagc
    47101 gcgataaaca ctttctgaaa acccatcgac ttcagttgcg tcacgtcgac ttctttcggg
    47161 tcgacaggat acgcatcaat accgccttca cgcagcccgg ctaacaccgc tgcgccagaa
    47221 ttcagagaaa cttcccgctc agcggaagtc ccgcccaaca ggaccgcgat tttatcagtc
    47281 atgttgttct tcctccggag tttgcggctt cagtttgatt tcagctaaag aacgggcaat
    47341 ttttccgata ttaccagccc cctgaacgag aatcaggtcg ttaccggtta ataccggtgc
    47401 cagcatctcg gctacctgcg cagggtccgg caccagaatg ggatcaattt tcccacgtcc
    47461 acgaattgtg cgacacagcg aacggctgtc cgctcccgga attggcgctt cgccagccgg
    47521 atacacttcc agcatcaaca gggtatcaac ctgcgtcagc acattggcga aatcatcata
    47581 caggtcgcgc gtacgggtaa aacggtgcgg ctgaaacagc attaccaggt ttttatccgg
    47641 ccagcctgcg cgcgccgctt taatggtggc gtcaacttcc gtcgggtggt ggccgtagtc
    47701 atcgaccagc attgccgtac cgcttttacc attcactggc tccagcggga attcaccgag
    47761 gaaatcaaaa cggcgaccag tcccctggaa gctctccagc gcacgcaaaa tagcctcgtc
    47821 gtcaattccc tcttccgtag caaccgcaac cgcagctgcg gcgttcagcg cgttatgacg
    47881 gcctggcgca ttcagggtga cgcacatcgg ctctttgtcc tgacgcagca gcgtaaagtg
    47941 cccctgcggg ccaatctgct gataatcttc tacacgcacg tcggcatctt cgctgaagcc
    48001 gtaagttgtg gtctgacgtc ccacacgcgg taacaattcg cggatcaccg gatcatcaac
    48061 acacatcacc gcacgaccgt aaaaaggcag gttgtgcaga aaattaataa aggtctgttt
    48121 taaattctca aagtcgccct ggtaggtatc catgtggtcg gcttcgatat tggtgacaat
    48181 cgccaccatc ggttgcagat gcaggaacga tgcatcactc tcatctgctt cggcaatcag
    48241 gtaccgacca tgccccaaac gcgcatgaac ccccgccgct tttaccagcc cgccgttaac
    48301 gaaggttggg tcgagccccg cttctgcgta gatgctggaa accatcgcgg tagttgtcgt
    48361 tttgccgtgc gttcctgcaa tggcgatgcc atgacgaaaa cgcattaact cagccagcat
    48421 ttcggcacga cggatcaccg gaatacgcgc ttcatgagct gcgacaattt ccgggttgtc
    48481 ggcagaaatg gcgctggaaa caacgaccac gctggcatca cgtacgtttt ccgggcgatg
    48541 gttgaaataa atcgtcgcac ccagattcag taactgctgc gtgaccggat ttggcgctaa
    48601 atcggaacca ctgatctgat aaccttcatt ggccagaact tcggcaatac cgcccatacc
    48661 ggcaccacca atgccgacaa aatgtatgtg ccgaacgcga cgcatttcgg gcacgatgga
    48721 acgcagtttt gccaattgtt gtgtattcat tctttacgcc attaacttct taaattcata
    48781 cgatgcaaaa ggcatcgcta caattacgcc cgggcagccc ggctcacttc atttgccact
    48841 cgctcggtgg catccggaat ggatgcagcg cgggcgcgtt ctgccatggt taataaggtt
    48901 tctcgcgacc acccggccag ggtgttggcg acagcatcca cgctaagctg tggctgctcg
    48961 ataattttgg ctgcgcccgc tttttccagc ggtagcgcat tccagtattg ctggcggtct
    49021 ttatgttgaa acggcacaaa caacgccggt agtcctgccg cggcgatttc actcacagtt
    49081 aaagcaccgg agcggcaaac gacgacatcc gcccacgcat acgccgccgc catatcatca
    49141 ataaattcag tcactttatg ctgcggttgc cccgcttcgg catacgcctg ttcaacggat
    49201 tgttgcgaac ctttgccgct ctgatgccag atagtgactg aatcacccag tttcgcagca
    49261 acctgcggca ttgtctggtt aagaatgcgt gcgccctggg aaccacccac taccaacaca
    49321 cgaaccggac cttcacgtcc agccaaacgt tgctgcggca acggcagcgc cagcacatcg
    49381 gtacgcaccg ggttacccac cacttccgca ttagggaaag cgcctggaaa cgcctgcatc
    49441 actttggtag caatcttcgc cagccattta ttggttaagc ccgcaatacc gttttgttca
    49501 tgaagtacaa ccggaatgcc taacgaccac gcggccagac cacctggacc tgatacatag
    49561 ccgcccatac cgagtaccac gtcaggtttg tacgctttca taatcgcccg cgcctgacgc
    49621 caggcgttga agatacgcag cggcgcagct atcagtgctt ttataccttt tccacgcaaa
    49681 ccagagatac gaatgaaatc aatttcgatg ccatgttttg gcactaagtc cgcttccata
    49741 cggtcggcag tccccagcca gcgaacttgc caaccctgag ccattagata gtgcgcaacc
    49801 gccagtcccg ggaatacatg tccaccggtt ccgcctgcca tcaccattaa tcgctttcct
    49861 tgaccactca tcgtgaacct cgtacaaacg cctgcgcttt ctccagacgc gtttcataat
    49921 caatacgcaa cagcatcatg atggctgtcg acataatcag taagctcgaa ccaccgtaac
    49981 tgattagcgg caatgtcaga cctttggtcg gtaacatccc cgccgccgcg cctacgttaa
    50041 ccagcgcctg gaagctaaac cagatgccaa tagaacaggc gagaaaaccg gaaaaacggt
    50101 ggtcaatttc taatgctttg cggccaatcg acatcgcacg aaaagcgacg aagaatacca
    50161 ttaaaagtgc tagtaccaca ccgacataac ctaattcttc gccgataatg gcgaaaataa
    50221 agtcagtgtg cgcttccggc agatactcca gtttttgtac cgaattacct aaaccttgcc
    50281 cccaaagttc gccgcgacca aacgccatca gcgattgcgt taactgatag ccgctgccaa
    50341 agggatcttc ccacgggttc cagaatgcgg taacacggcg gatacggtac ggttcggcga
    50401 ggatcagcaa cacaaccgct gaaatgccca taccgataat ggcaatgaac tgccacaatt
    50461 tcgctcccgc caggaacaac atcgccagcg tagtcacaaa caacaccacc accgtaccaa
    50521 ggtctggctg tgccagcagt aacactgcca acaccagaat cacgcccatc ggtttcagga
    50581 agccgcgcag gttattacgt acttcgtcgc ctttacgcac cagatagttg gcgatatagc
    50641 aaaacagcga cagttttgtc agctccgcag gctggatacg cagcaaaccg agatcgatcc
    50701 aacgcgatgc ccctttaaca gcgctaccca ctaccagcac gatcatcagc aggatgatag
    50761 atccgagcag catcgtggca ctgtagcgtt gccagaactc catcggcaga cgcagcgtaa
    50821 tgatcgccag aataaacgcc aaaatcagat agacaccatc acgcttcgcg aagaagaacg
    50881 gatcgttggt taagcgttgc cctatgggca ttgacgccga ggtcaccatg ataaagccaa
    50941 tcgccgcgag gccgaaggtc agccacagta aggtgcgatc gtacatgatc aggctgtcgg
    51001 tatctttttc ccgcgagccc atcacccagc cctttagcgc cgtggagatc cagaccagga
    51061 tactgaatcc tggcaggcgc ggcattttca ggcgagggag agataaacgc atcaacctaa
    51121 ctccttcgcc agacgggcaa actcattgcc tcgttgttca aagttcttga actgatcaag
    51181 gctggcacag gccggggaga gcagaaccat atcgcccggc tgaacacgcg gagccagcaa
    51241 gcgcatagcc tgttccatag tttcggtttg ttctgccact tccgggcgta gcgccgccag
    51301 ctgcgcgccg tcacgaccga aacaatacag acgtacgtta tcgccattca ggtaacgcac
    51361 cagtgggcta aagtccgccg atttaccatc gccgcccagc aacaaatgca gcgtgccgtc
    51421 tacgtgcagg ccattcagcg ccgcttccgt actgccgacg ttggtcgctt ttgaatcgtt
    51481 aatccagcgc acaccgttat gctccagcac cacttcaaag cgatgcggca gaccggtgaa
    51541 ggtggttaac gctttcaggc tgctggcgcg cggtaaccct gcggcatctg ccagcgccag
    51601 cgccaccagc gcattggtgt agttatgctg cccggaaagt ttcatctctt tcacattcag
    51661 cactttctcg cctttaaccc gcagccaggt ttcgccctgc tgatgattca ggtgatagtc
    51721 acccatgttg acgccaaagc tgacgcagcg ttcatccgca ccgcgaatcg gcattgttaa
    51781 ggcatcatca gcattaacca cgcaaacttt cgcgttttcg taaatgcgca gttttgctgc
    51841 acgatactgt tgtaaaccaa acgggtagcg atccatatga tcttcagtca cgttcagaat
    51901 ggtcgctgct accgcctgta agctggaggt ggtttccagc tggaagctcg acagttccag
    51961 cacgtacagt tcacactcat catccagtag catcaacgca ggcaggccaa tattgccacc
    52021 cacaccaacg ttaacccccg ccgctttcgc catttcaccc actagcgtgg tgaccgtgct
    52081 tttgccgtta gaaccggtaa tcgccacaat cggtgcttgt gcttcgcgac aaaacagctc
    52141 gatatcgcca acgatttcga ttccggcatc agcggcagcg cttaaggatg gatgcgccag
    52201 tgcaataccg ggactggcga caatcagatc tgccgccatc agccattcat cattcagact
    52261 gcccgtgtgg cgttctacgg cttcgggtaa tttatccagg ccaggcggtg tcatacgcgt
    52321 atccataacg cgcggcgtca caccgcgagc gaggaaaaag tccacgcagg aaagcccggt
    52381 gaggcccagg ccgataatga caacattttt accctggtaa tcagccatga ttaacgtacc
    52441 ttcagcgttg ccagaccaat caggaccagc atcagcgaaa taatccagaa acgcacaatg
    52501 acgcgcggtt ccggccagcc tttcagttca tagtggtgat gaatcggtgc catgcggaaa
    52561 atacgttgtc cgcgcagttt aaaggagccg acctgcagga tgacagaaag cgtttctacc
    52621 acgaacacgc cccccataat caccagcagg aattcctgac gtagcagtac ggcgataatg
    52681 cctaacgcac cacctaacgc cagcgaaccg acatcgccca taaagacctg cgccggatag
    52741 gtgttaaacc acaggaagcc cagtcctgcc ccgactatcg cggtacagac aataaccagt
    52801 tccccggcgt gtcgcagata cggtatgtgt aaatagctgg caaagttcat gttgccggtc
    52861 gcccacgcca ccagcgcaaa accaccggcg acaaatacgg tcggcataat tgccagaccg
    52921 tcaagaccat cggtcaggtt taccgcgttg ccagtaccca caatgacgaa gtaagccagc
    52981 agaatgtaga acagccccag ttgcggcatc acatctttaa agaatgggac caccagctgc
    53041 gttgcgggcg tgtctttgcc ggcaaggtac aaagcgaaag caacacccag cgcaatgacc
    53101 gacatccaga aatatttcca acgagcgatc aaccctttgg tgtctttacg caccactttg
    53161 cgataatcat caacaaagcc aataacaccg taacctacca gcaccaccaa cacgcaccag
    53221 acgtacggat tggacgggta agcccacagc agcacggaga tcacaatcgc tgtcaggatc
    53281 ataatcccgc ccatggtcgg cgtaccgcgc ttgctgaagt gtgattcagg accgtcgtta
    53341 cgcactacct gaccaaagga aagtttttgc aaatgagcaa tcatgcgcgg gcccatccac
    53401 aatgagatga acagcgcggt cagcaggctg acgatggcgc gaaacgtcag ataggaaaag
    53461 acgttaaagc cggaataata tttgaccaaa tgttcggcca gccaaactaa catgtcccat
    53521 tctcctgtaa agcgcgtact acctcttcca tggcagcact acgtgaaccc ttaactaaaa
    53581 tcgtaattac ctgttgctca gcaatcagtg atttaagacg cgtaattaac gcagttttat
    53641 cagcaaaatg ttcgccaacg ccgctggcgg tgctgatagc atggctttgt ttacccatgc
    53701 ttaacacgcg gtcaatacca gcagcttttg ccgcctcacc cacctgtaca tggcaggctt
    53761 cgctttcagc gcccagttcc gccatatcgc ccaccaccag cacgcggtag cccggcattt
    53821 cagccagtac ctgaacagct gcggtcattg aaccgacatt ggcgttgtag gagtcgtcga
    53881 gcagcaactg gttttctgcc agttggatgg ggaacagacg gccaggaaca gctttcagat
    53941 ttgccagccc cgctttgata gcatcaagcg ttgcgcccac ggacatggag agcgcagcgg
    54001 ctgccagcgc attcgcaata ttgtgacgcc ccggcaacgg cagcagaaca tcgacgctac
    54061 cggttggggt ttgtagggta aattccatac cgtgcgaggt cacatggata ttggtggcgg
    54121 tgaaatcgct gttggcggca ttgggtgaga aacgccacac tttgcgtgag ccaattacgc
    54181 tctgccagtt cagccagtcg ttgttgtcgg cgttcataat ggcgataccg ttttccggca
    54241 ggccgctaaa gatttcacct ttcgctttcg cgacacccgc aagcgagcca aaaccttcca
    54301 gatgcgccgc tgccaggttg ttgaccagcg ccgcttccgg gcgagtcaga ctcacagtcc
    54361 aggcaatctc gccctgatgg ttcgcgccaa gttcaataac tgcgtaatcg tattccggcg
    54421 ttaagcgcaa cagcgtcatc ggtacaccga tgtcgttgtt gagattgcct gccgtataaa
    54481 gcgtgttgcc gcactggctt aaaatcgccg ccgtcatctc tttaacggag gttttgccgg
    54541 aggaccccgt cagagcaacc acgagcgccg gaacttgctg gcgaacccat gcagccagtt
    54601 caccaaacgc cagacgcgta tccttgacga ttaactgcgg caggtcgatg tccagcggac
    54661 ggctaaccag tagtgcgcct gcgccgccag ctttcgcctg gtcggcaaaa tcgtgggcat
    54721 caaaacgttc acctttcagg gcaacaaaca ggcagcccgg cgtcagtttt cgggtatcag
    54781 tggttacagc atcaagggtg atatctgcac cttgcagttc accgttgaga atatcggtaa
    54841 gttggctaag ggttacgcta atcatgcaat cccccccagc agacgcgcca ccgtgacgcg
    54901 atcggagtag tccagacgct gattgccaac aatctggtaa tcctcatggc ctttgcccgc
    54961 gaccagtacc acatcattct ctttagcctg cataacggcg caagtcaccg cttcagcacg
    55021 gccttccatc actttggcat gtccggcatc taacattccc gccagaatat cgttgatgat
    55081 ggcacgcggt tcttcggtac gcgggttatc gtccgtcacc accgccacgt cagcaaactc
    55141 ttcggcaatt gcgcccatca gtggacgctt acctttatcg cgatcgccac cacagccaaa
    55201 gacgcaccac agcttgcccg cacagtgcag gcgcgccgcc tgtaaggctt tttccagtgc
    55261 atccggcgta tgcgcgtaat ccaccaccac cgtcggtttg cctggcgcag tgaacacttc
    55321 catacgtccg caaaccggtt gcagacgcgc ggcggttttc agcagatcag ccagtggata
    55381 gcccagcgcc agcagtgttg ccagcgcgag caacaggttg ctgacgttaa aagcgcccat
    55441 cagatggctt tcaatttcgc catcgcccca gcttgaacta aagcgaatgg tcgcgccgct
    55501 gtcgtgatag ttcacttcgg tcgctttcaa ccagcgtccg tgacagttcg gattgatatg
    55561 atcttccatt gataccgcaa ccgcgtccgg cagttttgcc agccagcggc ggcccacttc
    55621 atcgtcggcg ttaataatcg cctgaccgca atgatgttca gaataaagca gccatttcgc
    55681 agcttcgtag tgttccatat caccatgata atcaaggtga tcgcggctta agttggtaaa
    55741 gaccgacgcc ggaaatttca atgccgccac acggtgctgt accagcccgt gggaggaaac
    55801 ttccattgcg caaaaagtcg cgccctgatc caccagcccc gccagctcat gctgaacatc
    55861 aactgccgaa ccggtagtat tttctgtcgg gatcactttc cccagcagac cgttaccaac
    55921 ggtgcccatt accgcgctgg tttcgccaag cagttggctc cactgcgcca gaagctgggt
    55981 cgtcgtggtt ttgccgttgg tgcccgttac gcctacgaga cgtaaattgt cagagggttc
    56041 atggtaaaag cggcccgcca gtgcagataa acgctcgttg agctggctga gatagatgac
    56101 cggtacgccg tgcatttcac ggatttcacc gtcggtcgcc tcatctttcg cctctgcaat
    56161 aatggcagcc acaccttgcg ctatcgcctg cgggatatat cgacgcccgt ccgcctgatg
    56221 acctactaca gctacaaaga gatcgcccgc cgcagccaca cggctgtcga gtgtcatctc
    56281 tcgcagtgct cgcgaaggtg cgtctggcac ccacggagca agaaggtcgc gcaaattacg
    56341 atctgccacc tgtcccctcg ccttgattaa tcacaaattc atttttatcg cccgttgtca
    56401 gcgcatccgg ctcgatgttc atggtacgca atacgccgcc catgatggca ccaaagaccg
    56461 gcgcggaaac ggcgccgccg tagtatttac ccgcctgcgg atcgttgata acaacaacca
    56521 gcgcgaagcg cggctgactc gcaggcgcaa cgcctgcggt ataagcaata tatttattga
    56581 tgtagcgacc gtccggcccg acctttttcg cggtaccggt tttaatggcg atacgatagc
    56641 ctttaatcgc cgccttcacg ccgccgccgc ctggtagcgc cacgctttcc atcatatgca
    56701 ccacggtgcg aacaatggat tccgggaaga cacgttcacc gggaaccggg gggtcaactt
    56761 tggtaatcga cagtgggcga taaatgccgt agctgccgat agttgcgtag actcgcgcta
    56821 actgtaacgg tgttaccatt agcccgtagc cgaaagagaa ggtggccctc tctatgtcag
    56881 accaccgttg tttttgagga tataagccac tgcgttctcc gaccaacccc aaattggtcg
    56941 cttttcccag tccaaaacgt gagtaagtat ctactaacac tgaggacggc atcgttaacg
    57001 ccagcttgga aacaccgacg ttactcgact tctgtaatac cccggtcagg gttaattcgc
    57061 tgtagcgtgc cacgtctttg atttcgtggc cgttaattcg ataaggaatg gtattgagta
    57121 ccgagttttc ccgcaccacg ccacgttgca acgcggtcat caccaccatc ggtttaaccg
    57181 ttgagcccgg ttcaaacacg tcggtgatgg tacggttacg catcgcctct ttcggcgtgc
    57241 cgctcagatt gttagggttg tatgacgggc tgttagccat cgccagcact tcacctgtgt
    57301 tgacatccac cagcacggcg ctaccagatt cagccttgtt aaaggccacc gcgttgttca
    57361 gttcgcgata aaccagcgcc tgcaggcgtt catcaatact cagcgccagg ttgtgcgctg
    57421 cctggctgtc agtagaagaa atatcttcaa ttacgcgacc atagcggtct ttacgcacaa
    57481 tgcgctcacc cggctgcccg gtaagccatt tatcgaaact cttctcaacg ccctcaatcc
    57541 cttgactatc gacgttagta aagccgatga ggtgagcagt cacttcgccg gacggatagt
    57601 aacggcgaga ctcttcacgc agatgaatcc ccggcagttt cagttttttg atgtagtccg
    57661 ccatgtcaga gtttacctga cgcgccagat aaataaagcg ccctttcggg ttggcgttaa
    57721 tgcgggcaga aagctgatcc agcggaatat tgagcgcgtt agccagcgcc ttccagcggt
    57781 caccgacgct gataccgcca gcgtcatgca cttctttcgg gtcagcccaa atcgctttta
    57841 ccggcacgct caccgctaac gggcgaccag aacggtcagt aatcatgccg cgggaggtgg
    57901 aaacttgctg aacgcgaaga gaacgcatgt cgccctcttt caccagcata tccggggaga
    57961 taacttgtaa ccacgctacg cgtccgagca gaaaagccag cgccaggaga atacagccgc
    58021 ataacaacgc aaaacgccaa ctgataaagt tggcatgttc ttcctgacgt tttggtttct
    58081 gcgttttcgc cgctgctttc atgcgtcgcg tttatcctta tttttgcact acgatatttt
    58141 cttgtgacgg atcaacatgc tgcatttgca gcttttccgt ggcgatcctt tccacccggc
    58201 tatggtcgcc gagcgcattc tcttcaagga tcaggttgcg ccattcaatg tctaaagcat
    58261 ctcgctccag caccagttgt tcgcgctgag cggtcagtaa acgggtatgg tgcgccgtgg
    58321 ttaccacggt caccgccgtc aaaataatgc aaatgaacag gcagagtggc agcttcccaa
    58381 atcgcaaaag atcgtcaccg ataacaccag gcaatgcatg gcgctcgtgg cttcccatcg
    58441 atcctttaac tttgcttaga gcttctgtca ctctgctgat catgcgttcg tcctctctgc
    58501 aatacgcaga actgaactac gggcacgagg gttctcagcc acctcttctt cgcccggcat
    58561 taacttgcct agtgctcgca gctgacggcc acccagtttt ttgagctgct cttcagtcat
    58621 cggtaaccct gccggaactt gcggaccgcg gctgttttca cgcataaaac gtttcacaat
    58681 acggtcttcc agcgagtgga agctgatgat cgaaagccgc ccacccgggg ccagcacgtt
    58741 gagcgagctt tttagcgcct gctctatctc ctccagttca ctgtttaccc aaatgcgcac
    58801 cgcctggaag gtacgggtcg cgggatgttt aaacttatct ttcaccggcg ttgcagcagc
    58861 cacgacttcc gccagttctt tggtgcgggt catcggctgt tcgcggttac gctcgacaat
    58921 ggcgcgggca atgcgtttgg caaaatgctc ttcaccatag gttttcaata cccaggcgat
    58981 atcggcttct tctgcggttt gtagccattc agcggctgac tgcccacggg ttgggtccat
    59041 acgcatgtcc agcggaccat cgcgcataaa ggaaaagccg cgttcagcat catcaagttg
    59101 cggtgaagag acgccaagat cgaggagaat gccgtcgatc ttgccaataa gatcgcgctc
    59161 ggcaacgtat tcgcccagcg cggagaaagg tccgtggatg atggagaagc gcggatcatc
    59221 aatagtcttc gcaacggcga tagcctgcgg gtcgcgatcg atcgccagca aacgcccctc
    59281 ttcgccaagc tgcgagagga tcagacgtga gtgaccaccg cgaccaaaag tcccatcaat
    59341 gtagatgcca tcaggacgga tattgaggcc attaacggct tcatccagca gcaccgtagt
    59401 atgtttatag ttttccatca ttttatagag acaagtcctg cagtcgctcc gataagtctc
    59461 cggtagccaa ctgctctgcg tcgatatctt ccttgacctg ttgatgccag gttgtttcat
    59521 cccacagctc aaacttgttg aactgtccaa ccagcatcac ttcttttgtc agcccggcat
    59581 gttgccgcag tactggcgcg attaacaatc gacctgcgcc atccatctga cattcgctgg
    59641 catgacctaa cagtaggcgc tgcacacggc gctcaaccgg gttcatgctc aacagacgcg
    59701 ataatttttg ctcgataatt tcccattcag gcagggggta aagcagcagg cacgggtgat
    59761 gaatgtcaat ggtgcaaacc atttgaccgg cagcgttctc aagcagctgt tcccgataac
    59821 gggtaggcac tgataagcgc cctttgctgt cgagattgac taacgttgct ccccggaaca
    59881 tgccagcctc accccttatt cccactttgc cccacaattt cccactgaaa ggagtttacg
    59941 gagcggagga aaagcttgtc aagccagtca caaggcatac cgaggtaaaa aacccgatgt
    60001 ttacggctaa tatcagcgtt gtttatttta cgtccaacgt gcgaagcaaa ttaacgttat
    60061 gaatagctgt aagaaaaaat ccctcatcgt catcactgtt ttaaaatgac tcactatttt
    60121 ccacagcaaa tataaagtgt cagtttgcga cgcgagcggc attttaggac atatcttccc
    60181 cggttaacag tccttgttac gtctgtgtgg cgcttgatgc cagcgggttt gcgcaaaagc
    60241 aaagaaaagt gtttgttaaa tcggcaattc atcgccgcat gtaacaaaat aatgcaaaaa
    60301 cgcgctgtcg atatcactta attattgtga atttcagctg gtgagttaat taacgggcgg
    60361 gtttaaaacc atttatcaat aatagtcagg aatagtctta tttactttaa ggatattgat
    60421 g
//
LOCUS       DAJTUI010000013        59591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTUI010000013 DAJTUI010000000
VERSION     DAJTUI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.013"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            265..1554
                     /gene="sdaC"
                     /locus_tag="OO464_000882"
     CDS             265..1554
                     /gene="sdaC"
                     /locus_tag="OO464_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6226128.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1612..2979
                     /gene="sdaB"
                     /locus_tag="OO464_000883"
     CDS             1612..2979
                     /gene="sdaB"
                     /locus_tag="OO464_000883"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6226129.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3090..3845
                     /gene="xni"
                     /locus_tag="OO464_000884"
     CDS             3090..3845
                     /gene="xni"
                     /locus_tag="OO464_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6226130.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(3898..5046)
                     /gene="fucO"
                     /locus_tag="OO464_000885"
     CDS             complement(3898..5046)
                     /gene="fucO"
                     /locus_tag="OO464_000885"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR6226131.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5074..5721)
                     /gene="fucA"
                     /locus_tag="OO464_000886"
     CDS             complement(5074..5721)
                     /gene="fucA"
                     /locus_tag="OO464_000886"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6226132.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6268..7584
                     /gene="fucP"
                     /locus_tag="OO464_000887"
     CDS             6268..7584
                     /gene="fucP"
                     /locus_tag="OO464_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6226133.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7617..9392
                     /gene="fucI"
                     /locus_tag="OO464_000888"
     CDS             7617..9392
                     /gene="fucI"
                     /locus_tag="OO464_000888"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR6226134.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9501..10920
                     /gene="fucK"
                     /locus_tag="OO464_000889"
                     /pseudo
     CDS             9501..10920
                     /gene="fucK"
                     /locus_tag="OO464_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            10922..11344
                     /gene="fucU"
                     /locus_tag="OO464_000890"
     CDS             10922..11344
                     /gene="fucU"
                     /locus_tag="OO464_000890"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6226135.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11402..12133
                     /gene="fucR"
                     /locus_tag="OO464_000891"
     CDS             11402..12133
                     /gene="fucR"
                     /locus_tag="OO464_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6226136.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12177..13277)
                     /gene="rlmM"
                     /locus_tag="OO464_000892"
     CDS             complement(12177..13277)
                     /gene="rlmM"
                     /locus_tag="OO464_000892"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6226137.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13270..13665)
                     /locus_tag="OO464_000893"
     CDS             complement(13270..13665)
                     /locus_tag="OO464_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6226138.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13684..14601)
                     /gene="gcvA"
                     /locus_tag="OO464_000894"
     CDS             complement(13684..14601)
                     /gene="gcvA"
                     /locus_tag="OO464_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6226139.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(14952..15179)
                     /locus_tag="OO464_000895"
     CDS             complement(14952..15179)
                     /locus_tag="OO464_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6226140.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15371..16576
                     /gene="csdA"
                     /locus_tag="OO464_000896"
     CDS             15371..16576
                     /gene="csdA"
                     /locus_tag="OO464_000896"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6226141.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16576..17019
                     /gene="csdE"
                     /locus_tag="OO464_000897"
     CDS             16576..17019
                     /gene="csdE"
                     /locus_tag="OO464_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6226142.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17070..17876)
                     /gene="tcdA"
                     /locus_tag="OO464_000898"
     CDS             complement(17070..17876)
                     /gene="tcdA"
                     /locus_tag="OO464_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6226143.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18114..19211)
                     /gene="mltA"
                     /locus_tag="OO464_000899"
     CDS             complement(18114..19211)
                     /gene="mltA"
                     /locus_tag="OO464_000899"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6226144.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19420..19496
                     /locus_tag="OO464_000900"
     tRNA            19420..19496
                     /locus_tag="OO464_000900"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19454..19456,aa:Met,seq:cat)
     gene            19530..19606
                     /locus_tag="OO464_000901"
     tRNA            19530..19606
                     /locus_tag="OO464_000901"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19564..19566,aa:Met,seq:cat)
     gene            complement(19681..20934)
                     /gene="amiC"
                     /locus_tag="OO464_000902"
     CDS             complement(19681..20934)
                     /gene="amiC"
                     /locus_tag="OO464_000902"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6226145.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21166..22497
                     /gene="argA"
                     /locus_tag="OO464_000903"
     CDS             21166..22497
                     /gene="argA"
                     /locus_tag="OO464_000903"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6226146.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22559..24385)
                     /gene="recD"
                     /locus_tag="OO464_000904"
     CDS             complement(22559..24385)
                     /gene="recD"
                     /locus_tag="OO464_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6226147.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24385..27927)
                     /gene="recB"
                     /locus_tag="OO464_000905"
     CDS             complement(24385..27927)
                     /gene="recB"
                     /locus_tag="OO464_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6226148.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(27920..30808)
                     /gene="ptrA"
                     /locus_tag="OO464_000906"
     CDS             complement(27920..30808)
                     /gene="ptrA"
                     /locus_tag="OO464_000906"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6226149.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(30984..34352)
                     /gene="recC"
                     /locus_tag="OO464_000907"
     CDS             complement(30984..34352)
                     /gene="recC"
                     /locus_tag="OO464_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6226150.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34365..34715)
                     /locus_tag="OO464_000908"
     CDS             complement(34365..34715)
                     /locus_tag="OO464_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6226151.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(34700..35107)
                     /locus_tag="OO464_000909"
     CDS             complement(34700..35107)
                     /locus_tag="OO464_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6226152.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35104..35667)
                     /locus_tag="OO464_000910"
     CDS             complement(35104..35667)
                     /locus_tag="OO464_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6226153.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35658..36128)
                     /locus_tag="OO464_000911"
                     /pseudo
     CDS             complement(35658..36128)
                     /locus_tag="OO464_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36312..37106)
                     /gene="thyA"
                     /locus_tag="OO464_000912"
     CDS             complement(36312..37106)
                     /gene="thyA"
                     /locus_tag="OO464_000912"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6226154.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37113..37988)
                     /gene="lgt"
                     /locus_tag="OO464_000913"
     CDS             complement(37113..37988)
                     /gene="lgt"
                     /locus_tag="OO464_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6226155.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38139..40385)
                     /gene="ptsP"
                     /locus_tag="OO464_000914"
     CDS             complement(38139..40385)
                     /gene="ptsP"
                     /locus_tag="OO464_000914"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6226156.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40398..40928)
                     /gene="rppH"
                     /locus_tag="OO464_000915"
     CDS             complement(40398..40928)
                     /gene="rppH"
                     /locus_tag="OO464_000915"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6226157.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41613..42302
                     /gene="mutH"
                     /locus_tag="OO464_000916"
     CDS             41613..42302
                     /gene="mutH"
                     /locus_tag="OO464_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6226158.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42371..43084
                     /locus_tag="OO464_000917"
     CDS             42371..43084
                     /locus_tag="OO464_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6226159.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43222..43440
                     /gene="ygdR"
                     /locus_tag="OO464_000918"
     CDS             43222..43440
                     /gene="ygdR"
                     /locus_tag="OO464_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR6226160.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43548..44588
                     /locus_tag="OO464_000919"
     CDS             43548..44588
                     /locus_tag="OO464_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6226161.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44621..45811)
                     /gene="lplT"
                     /locus_tag="OO464_000920"
     CDS             complement(44621..45811)
                     /gene="lplT"
                     /locus_tag="OO464_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6226162.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(45804..47963)
                     /gene="aas"
                     /locus_tag="OO464_000921"
     CDS             complement(45804..47963)
                     /gene="aas"
                     /locus_tag="OO464_000921"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6226163.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48549..49580
                     /gene="galR"
                     /locus_tag="OO464_000922"
     CDS             48549..49580
                     /gene="galR"
                     /locus_tag="OO464_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6226164.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49587..50849)
                     /gene="lysA"
                     /locus_tag="OO464_000923"
     CDS             complement(49587..50849)
                     /gene="lysA"
                     /locus_tag="OO464_000923"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6226165.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            50971..51906
                     /gene="lysR"
                     /locus_tag="OO464_000924"
     CDS             50971..51906
                     /gene="lysR"
                     /locus_tag="OO464_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6226166.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(51893..52585)
                     /gene="ygeA"
                     /locus_tag="OO464_000925"
     CDS             complement(51893..52585)
                     /gene="ygeA"
                     /locus_tag="OO464_000925"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR6226167.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52714..54132)
                     /gene="araE"
                     /locus_tag="OO464_000926"
     CDS             complement(52714..54132)
                     /gene="araE"
                     /locus_tag="OO464_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR6226168.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54447..55208)
                     /gene="kduD"
                     /locus_tag="OO464_000927"
     CDS             complement(54447..55208)
                     /gene="kduD"
                     /locus_tag="OO464_000927"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6226169.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55238..56074)
                     /gene="kduI"
                     /locus_tag="OO464_000928"
     CDS             complement(55238..56074)
                     /gene="kduI"
                     /locus_tag="OO464_000928"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6226170.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56361..57542)
                     /locus_tag="OO464_000929"
     CDS             complement(56361..57542)
                     /locus_tag="OO464_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6226171.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            57797..59026
                     /locus_tag="OO464_000930"
                     /pseudo
     CDS             57797..59026
                     /locus_tag="OO464_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14702 a        16006 c        14539 g        14344 t
ORIGIN      
        1 ggcaaatatt gcccttaaat tctcttttac ttttgattta cagagtaaag cgttgggata
       61 atctatcttc caagcagatt attgtatttg ggatcaagat cactgataga tacataactt
      121 gtgtgtatct ttccgccctc aaattattac ggcggtaaat gattaagcca tcgccgatag
      181 acagatttca tttttacggt caggcacctt cccgggctga actggctaaa agctgaatta
      241 tttgcattcc tccaggagaa atagatggaa acgactcaaa ccagcacgat tgcgtcgaaa
      301 gactctcgta gtgcctggcg caagacagac accatgtgga tgctgggcct ttacggcacg
      361 gcaatcggcg cgggcgtgct gttcctgcca atcaacgccg gtgttggcgg tatgatcccg
      421 ctgatcatca tggctatcct tgcgttcccg atgacgtttt ttgctcaccg cggcctgact
      481 cgcttcgtac tgtctggtaa aaacccgggc gaagacatca ccgaggttgt agaagaacac
      541 tttggtattg gcgcaggtaa actgattacc ctgctctact tcttcgctat ctacccgatc
      601 ctgctggttt atagcgtggc aatcaccaat accgttgaaa gcttcatgtc tcaccagctg
      661 ggtatgacgc caccgccgcg tgcgattctg tcgctgatcc tgatcgtggg tatgatgacc
      721 atcgttcgct ttggtgagca gatgatcgtt aaagcgatga gtattctggt attcccgttt
      781 gttggcgtac tgatgctgct ggctctgtac ctgatcccgc agtggaacgg cgcagcactg
      841 gaaacgctgt ctctggacac tgcatctgca accggaaacg gtctgtggat gaccctgtgg
      901 ctggcaattc cggtaatggt gttctcgttc aaccactctc cgatcatctc ttctttcgcc
      961 gttgcgaagc gtgaagagta cggcgatatg gcagaacaga aatgctcgaa gatcctggca
     1021 ttcgcacaca tcatgatggt gctgaccgta atgttcttcg tcttcagctg cgtactgagc
     1081 ctgactccgg cagacctggc tgcggctaaa gagcagaaca tctcgattct gtcttacctg
     1141 gctaaccact ttaacgcacc ggttatcgcg tggatggctc cgattatcgc gattatcgct
     1201 atcaccaaat ccttcctcgg ccactacctg ggcgcacgtg aaggcttcaa cggtatggtg
     1261 attaaatctc tgcgtggtaa aggtaagtct atcgaaatca acaagctgaa ccgtatcact
     1321 gcgctgttca tgctggtaac gacctggatt gttgccaccc tgaacccgag catcctgggt
     1381 atgattgaaa ccctgggcgg cccaatcatc gcgatgatcc tgttcctgat gccgatgtac
     1441 gcaattcaga aagtaccggc aatgcgtaag tacagcggtc acatcagcaa cgtattcgtt
     1501 gtcgtgatgg gtctgattgc aatctccgca atcttctact ctctgttcag ctaagtcctt
     1561 tcgcgccgct ttcgggcggc gcttcctccg ttttaacgcg atgtatttcc tatgattagc
     1621 gtattcgata ttttcaaaat cggcattggc ccttccagtt ctcataccgt tggaccaatg
     1681 aaagcgggta aacaatttac cgacgatctg attgcccgta acctgcttaa agacgtgacc
     1741 cgcgtggtgg ttgacgtgta cggctcgctc tctctgactg gtaaaggcca ccacacagat
     1801 atcgccatta ttatgggcct ggcgggtaac ctgccggata ccgtggatat cgattccatc
     1861 cccggtttta ttcaggatgt gaatactcat ggtcgcctga tgctggcaaa cggtcagcat
     1921 gaagtggagt tcccggttga tcagtgcatg aacttccatg cagacaacct ttctctgcat
     1981 gaaaacggta tgcgcattac cgcgctggcg ggcgataaag tcgtttacag ccagacttac
     2041 tactctattg gcggtggctt tatcgttgat gaagagcatt ttggccagca ggatagcgca
     2101 ccggttgaag ttccttatcc gtacagttca gcagccgatc tgcaaaaaca ttgtcaggaa
     2161 accgggctgt cactctctgg cctgatgatg aaaaacgagc tggcgctgca cagtaaagaa
     2221 gagctggaac agcacctggc gaacgtctgg gaagtcatgc gtggcggtat tgagcgcggt
     2281 atttccaccg aaggcgtgtt gcctggcaaa ctgcgcgttc cacgccgtgc tgcggcacta
     2341 cgccggatgc tggtcagcca ggataaaacc accactgacc cgatggcggt tgttgactgg
     2401 atcaacatgt ttgcactggc agtgaacgaa gagaacgctg ctggcggtcg cgtggtgact
     2461 gcgccgacta acggtgcgtg cgggattatc ccggctgtgc tggcgtacta cgataagttt
     2521 atccgcgaag tgaacgctaa ctcactggct cgttacctgc tggtagccag cgccattggt
     2581 tctctttata agatgaacgc gtcgatttct ggtgccgaag tgggttgcca gggtgaagtt
     2641 ggcgtggcgt gctctatggc ggcagctggt ctggcagaac tgttaggcgc aagcccggcg
     2701 caggtttgca tcgcagcgga aatcgcgatg gagcacaacc tcggtctgac ttgtgacccg
     2761 gtcgcggggc aggttcaggt accgtgcatt gagcgtaacg ccattgcagc agtgaaagcg
     2821 gtgaacgccg cgcgtatggc actgcgccgt accagcgaac cgcgcgtctg cctcgataaa
     2881 gttatcgaaa ccatgtacga aacaggtaaa gacatgaacg ccaagtaccg cgaaacctct
     2941 cgcggcggcc tggcgatgaa gatcgttgcc tgcgattaat ctctcccaga ggcctcgttt
     3001 tgcgaagcct cttcctgttt tctcaaccag ccatagcctg ttccggcatc gaatgttacc
     3061 cttatcgcct gatctttaag ggggttatcg tggctgttca tttgcttatt gtcgatgcac
     3121 tgaatcttat ccgtcgcatt catgccgttc agggttcgcc ctgtgtcgaa acctgccagc
     3181 atgcgctcga tcagctcatt atgcacagcc agccaaccca cgcggtcgcc gtttttgatg
     3241 atgaaaaccg cagtagcggc tggcgtcatc agcgtttacc agattacaaa gcggatcgac
     3301 cgccaatgcc ggaagagttg cacgacgaga tgcctgcatt acgcgccgcc tttgagcaac
     3361 gcggcgtccc gtgctggtca gccagcggca acgaagccga tgacttagcc gccacactgg
     3421 cggtcaaagt gacacaggcc gggcatcagg caacgattgt ttcgacagat aaaggctact
     3481 gtcagttact ttcaccgaca ttacgtattc gtgattactt ccagaaacgt tggctggatg
     3541 cgccatttat cgataaagaa tttggcgttc aaccgcagca gttgcccgat tactggggac
     3601 ttgcggggat cagcagttca aaggtaccgg gtgttgcggg aatcggacca aaaagcgcca
     3661 cgcagctgct ggtcgagttt cagagtctgg aagggatata tgagaatctg gatgcggttg
     3721 ccgaaaagtg gcgcaaaaaa ttagaaaccc ataaagagat ggcgtttctg tgccgcgata
     3781 ttgcccgctt acaaaccgat ttgcatatcg acggcaattt acagcaattg cggttggtac
     3841 ggtaacggcg agccggatac gccgcaaatc gtatccggca ttatcacatc agcgcattta
     3901 ccaggcggta tggtaaagct ctacaatatc ctcaagcgtt gcttcacgcg ggttgccacc
     3961 ggtacaaaca tcatccagtg ccgcctgagc cagtgccgaa atgtcttcct tgcgtacacc
     4021 aacatcacgc aaatgtggcg gaataccgac atcacggttg agggcaaaca ccgcttcaac
     4081 agcggcatta cgcgcccctt ccaggctcat accttccact ttcacgccca taacgcgcgc
     4141 gatatcgcgg tacttctcac cggtaaagtc agcgttatag cgcatgacat gcggtaacag
     4201 gatggcgttc gcaacaccgt gtggagtgtt ataaaacgcg cccagtggat gcgccatacc
     4261 atgcaccaac cctaacccaa cattcgagaa gcccataccc gcaacatact gcccgagcgc
     4321 catttcttct ccggcatcct tatcaccagc aaccgatcct cgcagcgccc cagcaatgat
     4381 ttcaatcgct ttaatgtgca gtgcatcggt tagtgcccac gcgccacggg taatataccc
     4441 ctcaatagca tgagtgagcg catcaacacc cgtcgcggct ttcagcgccg gaggcatacc
     4501 atccatcatg tcagcatcaa taaacgccac ctgcgggata tcatgcggat caacgcaaac
     4561 aaacttgcgc cgtttttctt cgtcagtgat cacgtagtta atggtcactt ctgccgcagt
     4621 acctgctgtg gtggggattg ccagaatcgg tacactgggt ttattggtcg gggaaagccc
     4681 ttccaggcta cgcacatcgg caaactccgg gttgttgctg ataatgccaa ttgctttaca
     4741 agtatcctgt ggagaaccac caccaatagc gatcaggtaa tccgcgccgc tattctggaa
     4801 tacaccgagc ccttctttga cgacagtaat tgttgggttg ggcactacgc cgtcgtaaat
     4861 cgcccatgcc agccctgcag catccatctt atcggtcact ttcgccacca cgccgcattg
     4921 caccagcgtt ttatcggtga cgatcagcgc cttctgataa ccacggcgtt tcacctcatc
     4981 ggttaaagcc ccaacagcac cccgaccaaa ccatgccgtt tcgttcagaa tcattctgtt
     5041 agccatcatc cttctccttg ttgctttacg aaattactct tcaatccgta acccataggt
     5101 tttgaatttc tccagcacta cggcaatctc ttcatcgctc agcactggca ccgggtccgt
     5161 aatcgccagg gtcgtcaggt aaagttgcgc cagcacttca acttcatgcg ccagccataa
     5221 cgctttttcc agattcacct cacaagcgat aagcccatga tgttgtaaca aagttgcctt
     5281 acgatttttg agagccagcg caacatgttc agaaagttcg cgtgttccaa aggtcgcata
     5341 aggcgcgcaa ggaatagaat taccgccagc cgccgcaatc atgtagtgaa tagcggggat
     5401 cgatcggtta agaatggaaa ctgccgtgca atgaacggca tgattgtgaa caaccgcgtt
     5461 ggcatccggt ctgctttgat aggctgccat atggaaacgc cattcgcttg aggggagctt
     5521 tccttcctca tgtttaccgt tgccatcaat aaagacaata tgcgactccg tcagtttttc
     5581 atatggaatg cctgtaggcg taatcagcat cccatcctga taacgtacac tgacgttccc
     5641 cgctgtcccc tggttcagtc ccaggcgggt catttccagg caagtgtcaa taatctgacg
     5701 agcaagttta tttcgttcca ttagctacct ctctctgatt caaaacaggg caataatgtt
     5761 gttcctttca cactattgaa ttagccgttt aattacccac catcttcttc ctgattaaca
     5821 agaaagaaat tcacaagctt atattttgtg acctagctca actaatcaca gtaaataact
     5881 gcaagttctc tttttataac cccgttaaaa atgaccgctc ttaaaaatat ttatcaaaac
     5941 ggtcattttt ccatttcccc tcgccagaaa tgcccgtttt cggcacaaac aattaatacg
     6001 gtcatctggt ttgtgttttt tatgatttat tttctgaaac gggcatgaaa tttcgattat
     6061 taaagtgatg gtagtcacat aaagtcacct tccagctaat aagtgtgacc gccgtcatat
     6121 tacagagcgt tttttatttg aaaatgaatc catgagttca tttcagacag gcaaatattc
     6181 accgatatga agcccgaact cgctggtttt gcacttttga aaacataacc gattacgtgc
     6241 ttaagcttct gaacctaaga ggatgctatg ggaaacacat caatacaaac gcagagttac
     6301 cgtgcggtag ataaagatgc agggcaaagc agaagttaca ttattccatt cgcgctgctg
     6361 tgctcactgt tttttctttg ggcggtagcc aataacctta acgacatttt attacctcaa
     6421 ttccagcagg cttttacgct gacaaatttc caggctggcc tgatccaatc ggccttttac
     6481 tttggttatt tcattatccc aatccctgct gggatattga tgaaaaaact cagttataaa
     6541 gcagggatta ttaccggatt atttttatat gccttgggcg ctgcattatt ctggcccgct
     6601 gcagaaataa tgaactacac cttattttta gttggcctat ttattattgc agccggatta
     6661 ggttgtctgg aaactgccgc aaaccctttt gttacggtat tagggccgga aagcagtagt
     6721 cacttccgct taaatcttgc gcaaacattt aactcgtttg gcgcaattat cgcggttgtc
     6781 tttgggcaaa gtcttatttt gtctaacgtg ccacatcaat cgcaagacgt tctcgataaa
     6841 atgtctccag agcaattgag tgcgtataaa cacagcctgg tattatcggt acagacacct
     6901 tatatgatca tcgtggctat cgtgttactg gtcgccctgc tgatcatgct gacgaaattc
     6961 ccggcattgc agagtgataa tcacagtgac gccaaacaag gatcgttctc cgcatcgctt
     7021 tctcgtctgg cgcgtattcg tcactggcgc tgggcggtat tagcgcaatt ctgctatgtc
     7081 ggcgcacaaa cggcctgctg gagctatttg attcgctacg ctgtagaaga aattctaggt
     7141 atgactgcag gctttgccgc taactattta accggaacca tggtgtgctt ctttattggt
     7201 cgtttcaccg gtacctggct catcagtcgc ttcgcaccac acaaagtcct ggccgcctac
     7261 gcattaatcg ctatggcact gtgcctgatc tcagccttcg ctggcggtca tgtgggctta
     7321 atagccctga ctttatgcag cgcctttatg tcgattcagt acccaacaat cttctcgctg
     7381 ggcattaaga atctcggcca ggacaccaaa tacggttcgt ccttcatcgt tatgaccatt
     7441 attggcggcg gtattgtcac tccggtcatg ggttttgtca gtgacgcggc gggcaacatc
     7501 cccactgctg aactgatccc cgcactctgc ttcgcggtca tctttatctt tgcccgtttc
     7561 cgttctcaaa cggcaactaa ctgaacatat tttccgaata aagtgaggaa tctgtaatga
     7621 aaaaaatcag cttaccgaaa attggtatcc gcccggttat tgacggtcgt cgcatgggtg
     7681 ttcgtgagtc gcttgaagaa caaacaatga atatggcgaa agctacggcc gcactgctga
     7741 ccgagaaact gcgccatgcc tgcggagctg ccgtcgagtg tgtcatttcc gatacctgta
     7801 tcgcgggtat ggctgaagcc gctgcttgcg aagaaaaatt cagcagccag aatgtaggcc
     7861 tcaccattac ggtaacgcct tgctggtgct atggcagtga aaccatcgac atggatccaa
     7921 cccgcccgaa agccatttgg ggctttaacg gcactgaacg ccccggcgct gtttacctgg
     7981 ctgcggctct ggcagctcac agccagaaag gcatcccggc attctccatt tacggtcatg
     8041 acgttcagga tgccgatgac acatcgattc ctgccgatgt tgaagaaaaa ctgctgcgct
     8101 ttgcccgcgc cggtttggcc gtcgccagca tgaaaggtaa aagctatctg tcgctgggcg
     8161 gcgtttcgat gggtatcgcc ggttctattg ttgatcacaa cttctttgaa tcctggctgg
     8221 gaatgaaagt ccaggcggtg gatatgaccg aactgcgtcg ccgtatcgat cagaagattt
     8281 acgacgaagc cgaactggaa atggcactgg cctgggctga taaaaacttc cgctatggcg
     8341 aagatgaaaa taacaaacag tatcaacgca atgccgagca aagccgcgca gttctgcgcg
     8401 aaagtttact gatggcgatg tgtatccgcg acatgatgca aggcaacagc aaactggccg
     8461 atattggtcg cgtggaagaa tcacttggct acaacgccat cgctgcgggc ttccaggggc
     8521 aacgtcactg gaccgatcaa tatcccaatg gtgacaccgc cgaagcgatc ctcaacagtt
     8581 catttgactg gaatggcgtg cgcgaaccct ttgtcgtggc gaccgaaaac gacagtctta
     8641 acggcgtggc aatgctaatg ggtcaccagc tcaccggcac cgctcaggta tttgccgatg
     8701 tgcgtaccta ctggtcacca gaagcaattg agcgtgtaac ggggcataaa ctggacggac
     8761 tggcagaaca cggcatcatc catttgatca actcaggttc tgctgcgctg gacggttcct
     8821 gtaaacaacg cgacagcgaa ggtaacccga cgatgaagcc gcactgggaa atctctcagc
     8881 aagaggctga cgcttgcctc gccgctaccg aatggtgccc ggcgatccac gaatacttcc
     8941 gtggcggcgg ttactcttcc cgcttcctta ccgaaggcgg cgtcccgttc accatgactc
     9001 gtgtcaacat catcaaaggc ctgggaccgg tactgcaaat cgcggaaggc tggagcgtgg
     9061 aattgccgaa ggatgtgcat gacatcctca acaaacgcac caactcaacc tggccaacca
     9121 cctggtttgc accgcgcctc actggtaaag ggccgtttac ggatgtgtat tcggtaatgg
     9181 cgaactgggg tgccaaccac ggagttctga ccatcggcca cgttggcgca gactttatca
     9241 cccttgcctc catgctgcgt atcccggtat gtatgcacaa cgttgaagag accaaagtgt
     9301 atcgtccttc tgcctgggct gcgcacggca tggatattga aggccaggat taccgcgctt
     9361 gccagaacta cggtccgttg tacaagcgtt aatacattac accctgatgt gatgcccggt
     9421 cgctccggct accgggcctg aacaagcaag agtggttagc cggataaaca atgttatccg
     9481 gctatattgc aggagcgatt atgaaacaag aagttatcct ggtactcgac tgtggcgcga
     9541 ccaatgtcag ggccatcgcg gttaatcggc agggaaaaat tgttgcccgc gcctcaacgc
     9601 ctaatgccag cgatatcgcg atggaaaaca acacctggca ccagtggtct ttagactgcc
     9661 attttgcaac gctttgctga ttgctgtcgg caaatcaata gtgaactgac tgaatgccac
     9721 atccgcggta tcgccgtcac cacctttagt gtggatggcg ctctggtaga taagcaaggc
     9781 aatctgctct atccgatcat tagctggaaa tgtccgcgaa cagcagcggt tatggacaat
     9841 attgaacggt taatctccgc acagcggttg caggctattt ctggcgtcgg agcctttagt
     9901 ttcaatacgt tatataagtt ggtgtggttg aaagaaaatc atccacaact gctggaacgc
     9961 gcgcacgcct ggctcttcat ttcgtcgctg attaaccacc gtttaaccag cgaattcact
    10021 actgatatca cgatggccgg aaccagccag atgctggata tccagcaacg cgatttcagt
    10081 ccgcaaattt tacaagccac cggtattcca cgccgactct tccctcgtct ggtggaagcg
    10141 ggtgaacaga ttggtacgct acagaacaac gccgcagcaa tgctcggctt acccgttggc
    10201 ataccggtga tttccgcagg tcacgatacc cagttcgccc tttttggcgc tggtgccgaa
    10261 caaaatgaac ccgtgctctc ctccggtaca tgggaaattt taatggttcg cagcgcctag
    10321 gttgatactt cgctgttaag tcagtacgcc ggttccacct gcgaactaga tagccaggca
    10381 tggttgtata acccaggtat gcaatggctg gcatccggcg tgctggaatg ggtgagaaaa
    10441 ctgttctgga cggctgaaac accctggcaa atgttgattg aagaagctcg tctgatcgca
    10501 cctggcgcgg atggtgtgaa aatgcagtgt gatttattgt cgtgtcagaa cgctggctgg
    10561 caaggagtga cgcttaatac cacgcggggg catttctatc gcgcggcgct ggaagggtta
    10621 actgcgcaat tacagcgcaa tctacagatg ctggaaaaaa tcgggcactt taaagcctat
    10681 gaattattgt tagtcggtgg aggaagtcgc aacacattgt ggaatcagat taaagccaat
    10741 atgcttgata ttccggtaaa agttctcgac gatgccgaaa cgaccgtcgc aggagctgcg
    10801 ctgttcggtt ggtatggcgt aggggaattt aacagcccgg aagaagcccg tgcacagatt
    10861 cattatcagt accgttattt ctacccgcaa actgaacctg aatttataga ggaagtgtga
    10921 aatgctgaaa acaatttcgc cgttaatttc tcccgaacta ttgaaagtgc tggcagagat
    10981 gggacatgga gatgaaatta ttttttccga tgctcacttt cccgcccatt cgatggaacc
    11041 gcaggtgatc cgcgctgatg gcctgttggt gagcgacttg ctccaggcga ttatcccgtt
    11101 atttgagctg gacagttatg ccccgccgct ggtgatgatg gcggcggtag agggtgacac
    11161 tctcgatcct gaagtagaac gacgttaccg taatgcgctt tcactacaag ccccgtgtcc
    11221 tgacatcatc cgcatcaatc gttttgcgtt ttatgaacgg gcgcaaaaag cctttgcgat
    11281 cgttatcaca ggcgaacgag cgaagtacgg gaatattctt ttaaaaaaag gggtaacacc
    11341 gtaatctcat accggtacgc ccgcatgacg cgggcggtta tcgaatgatg gggtgaaaaa
    11401 tatgaaagcg gcacgccagc aagcgatagt cgacctgctg ctgaaccata ccagcctgac
    11461 cacggaagct ctctctgaac agctaaaggt cagtaaagaa accattcgtc gcgatctcaa
    11521 tgaattacag acgcagggta aaattctgcg caatcatgga cgcgctaaat atatccaccg
    11581 tcaaaatcaa gacagtggcg atccctttca catcaggctg aaaagccatt atgcgcataa
    11641 agcagatatc gcgcgcgagg cgctcgcgtg gattgaagaa gggatggtga tagccttaga
    11701 cgccagttca acttgctggt atctggcacg ccagttgcct gacatcaaca ttcaggtctt
    11761 caccaatagc catccgattt gccatgaact cggtaaacgc gaacgcattc aactgatcag
    11821 ttccggcggc acacttgagc gcaaatatgg ctgttacgtc aatccctcgc tgatttccca
    11881 acttaaatcg ctggaaatcg atctgtttat tttttcttgt gaagggatcg atagcagcgg
    11941 cgcactgtgg gactccaatg cgatcaacgc tgattacaaa tcgatgctat taaaacgtgc
    12001 cgcgcaatcg ttgttattga ttgataaaag taaatttaat cgttcagggg aagcccgcat
    12061 cgggcatctg gatgaggtaa cgcacattat ttctgatgag cgccaggttg caacttcttt
    12121 ggtaacagcc tgacggaaag ggtagcaggc cggagacgac gccccggcct tgcctgttat
    12181 cgctcgtcgc gacgaccacc caccgcagcc cagatgcggc ggacgtgcac cgtcacttct
    12241 tcgcgatcgt gatacaactg ccgcgcctga atctgagcat ttatgccatg ttcatcaagc
    12301 tgtgcctgaa tatacgccag attgtgtgat acttcttcgt agcgtttttt catcggcagt
    12361 ttgagattga aaatggtttc acggcaccag ccattaacca gccactgcgc catcaatgcc
    12421 gcaactttag ccggtttttc aaccatatcg cataccatcc aggagatatt gctgcgtgtc
    12481 ggacggaatt tgaaaccgtc ttcccgcagc cacgtcacct gcccggtatc catcagactt
    12541 tgcgccatcg ggccgttgtc gacggaataa acccacatgt tgcgcttcac cagttggtag
    12601 gtccagccgc cagggcaagc gcctaaatcc accgcccaca tcccgttcgc caggcgttca
    12661 tcccactcat ccgcaggaat aaacacatga aatgcctctt ccagtttgag cgtggaacga
    12721 ctcggcgcct ctgccggaaa tttcaggcgc ggaatgccca tatagaacgg cgaattattg
    12781 ttgctgtatg agtaaccggt atagcagcat cctggtgcaa taaagaatac atgcacaacc
    12841 ggacgcttcg gcgtttcata gttcgccagc accccagcat cgcgcaaggc agcgcgtagc
    12901 ggaacagtga acttacggca gaatttcagc aactctttgc tttcgttggt atcggcaact
    12961 tcaacacgca gttcaccgcc cttctctact acaccctgta acatgccgac aatgggggta
    13021 atacgatctt ctggcggcaa atgctgcagg agttccccca ccacaaacca ctggcgggca
    13081 aaaattaatg aactgaacgg cagctcacgg attaacttat cgccatcatc aggttgataa
    13141 cattcataaa tgacataacc cgcattctct ttcacgcggg caaaaccgaa aatttcccgc
    13201 tggccggctt tatcggtaat ttctgcggcg cactcttttt caaagcccgg acggcacagc
    13261 aatacaacct tattcatgac ttacgccctt gcgctttaaa cggatagcac caactaacat
    13321 taacgcccag cccgcaagga agctcacgcc gccaaccggt gtgacaaacg cccacaaacg
    13381 cagatgggac agcgccaggc aataaaggct gccgctgaac agcaccgtgc ctaacgcgag
    13441 gaaaacgcta ctccagtaaa accagatact gatgcgacgc tgcattgcca ccgccagacc
    13501 taagatcgcc agcgtatgaa acgcctggta ttcgagaccg gtttggatcc agcccatctc
    13561 aacggccccc atggttttac ttaacacatg cgcaccaaaa gcgcccagtg ccacaaaaat
    13621 gaagccgcta atggcggcga aaatcagcat aaaacggctg gtcatggtcg taccctacgt
    13681 aaattattgt tcataacgaa agcggaattt ttcttgttca gcagcggctt tcgccaggat
    13741 ccattggcga aaggcggcta ttttacccag ttctgcctgg ctgtcatgac aaaccagata
    13801 aaaagcattt ttactgacca gaacatcatt aaacgggcaa acaagacgtc cggcctcgat
    13861 ttcagattgc gccatcacgt tatttgccag cgccactccc tgcccgtgaa tagccgcttg
    13921 cagcaccatg gcgctatgac taaaaattgg cccttgctga acgttgatat gattaaaccc
    13981 caactgtcgg gtatatgtct gccagtcacg gcgtgacgca tcatgtaata acgtatgttt
    14041 agccagatct tccggggtct tcaagggttt ttcgcctgtc agcagtagcg gcgaacacac
    14101 cggcaataaa tattcggcgt acagtttttc cacccgtagc cccggccagt tgccccgacc
    14161 ataaaatatc gccacatcaa catcatccgc cagcttatct tcctgacgat caaccgcctg
    14221 gattcgaacg tcaattcccg gataagctga attaaagctg gaaagtcgcg gaaccaacca
    14281 atgaatggcg aaactgggga gtaaactgac cgtcaacgcc cccttggcgc tacgggcctg
    14341 gagtttacgc gtcgcttcgg ttaattgcga aaatatctct ttgatatcga ggaaatagct
    14401 ttgcccttcc tcggtcagca ggagtgaacg attacggcgg cggaacagtt ttagccccaa
    14461 aaaatcctca agagacttga tttgatgact tactgcggcc tgggtcacaa aaagctcttc
    14521 tgctgcgcga gtaaaactta aatggcgtgc tgcggcatca aaaactcgta aggcatttag
    14581 cggtggtaat cgtttagaca tggcttttaa actttgatgt taaatgaatt taacaattag
    14641 atcacactgt gtaacctatt agttttttta atctgagcca ttataaattg tccgttgagc
    14701 ttctaccagc aaatacctat agtggcggca cttcctgagc cggaacgaaa agttttatcg
    14761 gaatgcgtat tctggtgaac ttttggctta cggttgtgat gttgtgttgt tgtgtttgca
    14821 attggtctgc gattcagacc atggtagcaa agctaccttt tttcacttcc tgtacattta
    14881 ccctgtctgt ccatagtgat taatgtagca ccgcctaatt gcggtgcttt tttttacctt
    14941 gcgatcgcga attactgttc cagttcgacc atctctttca cgtcagtacg gttgatctgc
    15001 tgtttgttgc cattagcgtc tttatacgaa atcataccga tatcgttatc agtctgtggt
    15061 ttgccgtcag agacgatggt acgtccgtca ttggtgtgca tcacatagtt cgaaccggaa
    15121 caggcgctca gggcaaaagt cagcatacag gcagaaataa ttgcggcagt ctttttcatc
    15181 atcttctcct taaaacgatt aataaatctt aatagtattg attaacaggc taaaattaac
    15241 gcctaacact attcagcata tgttacttac gcggcttcgc caggatatcc agataattct
    15301 gatggttagc actctccttg tatcaaagtg aattttgcgt cacgatcggt gcatcaagcc
    15361 gaggagtacc atgaacgttt ttaatcccgc gcagtttcgc gcccagtttc ccgcactaca
    15421 ggatgcgggc gtctatctcg acagcgccgc gaccgcgctt aaacctgaag ccgtggttga
    15481 agccactcga cagttttaca gcctgagcgc cggaaacgtc catcgcagcc agtttgccga
    15541 agcccaacgc ctgaccgcgc gttatgaagc tgcacgagag aaagtggcgc aattactgaa
    15601 tgcaccggat gataaaacta tcgtctggac gcgcggcacc actgaatcca tcaacatggt
    15661 ggcacaatgc tatgcgcgtc cgcgtctgca accgggcgat gagattattg tcagcgtggc
    15721 agaacaccac gctaacctcg tcccctggct gatggtcgcc caacaaacta gagccaaagt
    15781 ggtgaaattg ccgcttaatg cgcagcgact gccggatgtc gatttgttgc cagagctgat
    15841 tactccccgt agtcggattc tggcgttggg tcagatgtcg aacgtgactg gcggttgccc
    15901 ggatctggcg cgagcgatta cctttgctca ttcagccggg atggtggtga tggttgatgg
    15961 tgctcagggg acggtgcatt tccccgcgga tgttcagcaa ctggatatcg atttctatgc
    16021 tttctctggt cacaaactgt atggcccgac gggtatcggc gtgctgtatg gcaaatcaga
    16081 actgctggaa gcgatgtcgc cctggctggg tggcggcaaa atgattcacg aagtgagttt
    16141 tgacggtttc accactcaat ctgcgccgtg gaaactggaa gcaggaacgc caaatgtcgc
    16201 tggcgtcata ggattaagcg cggcgctgga atggctggca gattacgata tcaaccaggc
    16261 cgaaagctgg agccgtagct tagcaacgct tgcagaagaa gcgctggcga aacgtccagg
    16321 ctttcgttca ttccgctgcc aggattccag cctgctggcc tttgattttg ctggcgttca
    16381 tcacagcgat atggtgacgc tactggcgga gtacggtatt gccttgcggg ccgggcaaca
    16441 ttgcgctcag ccgctactgg cagaattagg cgtaaccggc acactgcgcg cctcatttgc
    16501 gccatataat acaaagagtg atgtggatgc gctggtgaat gccgttgacc gcgcgctgga
    16561 attattggtg gattaatgac aaacccgcaa ttcgccggac atccgttcgg cacaaccgta
    16621 accgcagaaa cgttacgcaa taccttcgca ccgttgtcgc aatgggaaga taaatatcgc
    16681 cagttgatca tgctggggaa acagcttccg gctttgccag acgagttaaa agcgcaggct
    16741 aaagagattg ccggatgcga aaaccgcgtc tggctgggat ataccgtggc tgaaaacggc
    16801 aaaatgcact tctttggcaa cagcgaaggg cgcattgtgc gcggcctgtt ggcggtgttg
    16861 ttgactgccg ttgaggggaa aaccgccgcc gaattacagg cacagtcgcc gctggcattg
    16921 tttgatgagc tgggattacg tgcgcagctt agcgcctcac gcagccaggg gttaaatgcg
    16981 ttaagcgagg cgattatcgc tgcgacgaag caggtttaag cacagaaccg ggtcggataa
    17041 gacgttcgcg tctcatccga cctgattgtt taaccctgac gcgccgcttt cgccatcatc
    17101 ttcttcagcg catgagaaac cgcaacaaac ccaaaggtgg cggtcaccat cgttgccgcg
    17161 ccaaatccag atgcacaatc catccgcttc ggcccttctg ccgtggcttt catcgcacac
    17221 accgtaccgt ctgactgcgg gtacaccagc gcttcagtag aaaacacgca atccacgccg
    17281 agcttacctt tactgttttt cactacgcca aaatcgcttt tcaggcgctc gcgcaacttc
    17341 gccgccagcg gatcctgaat cgttttcgcc agatcggtaa cctgaatctg cgtcgggtca
    17401 atctgcccac ccgcgccacc ggtcgtgacc agcgggattt tattgcgccg acaataagca
    17461 atcagcgccg ctttgggccg tacactatca atggcatcaa tcacgtacga ataaccggca
    17521 ctcatatatt gcgctacgtt atccggcgtc acgaaatcat ccaccaccgt tacacggcac
    17581 tccgggttaa tctggcgaat gcgctccgcc atgacttccg cttttgccag cccgacgtta
    17641 tcgcgcagag cgtgaatttg ccgattggta ttggtgacgc acacatcatc catatcgata
    17701 agcgtgattg cgccaatccc cgtgcgcgcc agcgcttccg ccgcccagga accgacacca
    17761 ccgataccaa ccacacaaat atgcgcgtca gcaaacagtt gcaacgcttt ttcaccatac
    17821 agacgcgctg tgccaccaaa acgctgacgc catgcatcac taattaccac agacatagca
    17881 cctcagaatt aaaaagggcg aggttatccc tcaccctaaa cgcataaatg cctgatgcgc
    17941 tacgcttatc aggcctacat aactcctgca atattttgga tttgcatgat ttgtaggccg
    18001 gataaggcgt tcacgccgca tccggtatga acaacgcgca cttagtcaat aatcaggatg
    18061 tgaggttacc cctcccctgt catatccgta aaaacggcat acagaatatc acatcagccg
    18121 ctaaagacgt tacctgcgcc cggagccgtt ttcagcaccc aaacccgtcc atagtggttg
    18181 taccaacctg cgcggtgtcc ggcttccggc ccgatccctt gatagatatc gaagtgttgg
    18241 cctttgattg cgcccccgac atccaacgcc accatcagac gcaattcgta ctgaccgtta
    18301 aatttgccgt tattatccag cagcggcact tctgccagca aggtagtacc tggcggaata
    18361 attgaacgat cggaggcaac agaagcacgg ccaaccagcg gcaccgcact tgcccctttc
    18421 accggtgcaa aagattgcgg tttaaagaag acgaaagacg ggttctgttc cagcagctcg
    18481 cgaacctcgg cttcactgtg tgtttcgccc caatgacgaa tcgcctgcat cgacatatct
    18541 tcttttttca cttcgccacg gtcgatcagc accttaccaa tgctgcgata ggcatgaccg
    18601 tttttccctg catagctgaa aaagttaagc ggactgccat caccaaagtc gatataccca
    18661 ctaccctgca catccataat gaagttatcc atcagagagt tgctgtaagc gaggatgtat
    18721 ttatcgccca atgccccggc gtagatctcc gcacgagacg gcagacgacc acgttttggc
    18781 ggcatacggt aaataggata ctggaactcg ccctggcggg tatggcgcgc ctgaattacc
    18841 ggcgtgtaat agcctgtaaa ctgcacgtta ccatagttgt cggcaccttc catctgccag
    18901 gcatcaatgc cgaactggcg catattgcgg gtatcaccgc ctgcgcgcag ccactcttgc
    18961 accgcgttat aaacattact ctggttgcca tacagacgcg gtgacgaatt acggatatgg
    19021 ttaatttgct cggcaaaatc accggcgtta atcggcgcgc caacggcatc tggctggttc
    19081 accagagaga aaggctgggt aaatttcccg tctttatatt gctgtccgcg atcggttggt
    19141 ttggaagagc aggcggcaag cattgccaca accgtgccca taagaaggta ctttacccaa
    19201 cgtcctttca ttgttctctt cttcaggtta aaaaataagg cgcaacgaag ataacaaacc
    19261 gccgcgttta atgaaatgag cagcctctcc ctgacgcaaa ttttgcacaa aaaataggct
    19321 ttagtggttt gtttttgttc aaaatcatgc taaatccgtg atcggggtaa aaaaaaggtt
    19381 gcatgaaaac gcgagcggag tatagtgcgc atccacggac gcggggtgga gcagcctggt
    19441 agctcgtcgg gctcataacc cgaaggtcgt cggttcaaat ccggcccccg caaccaatca
    19501 aatttgatga agtaaaagca gtacggtgac gcggggtgga gcagcctggt agctcgtcgg
    19561 gctcataacc cgaaggtcgt cggttcaaat ccggcccccg caaccaatta ttgaacaccc
    19621 taacgggtgt ttttttgttt ctgatctccc ataaaaaaag cgccattcag cgccttttta
    19681 tcatcccctt ctcgccagcg ttgccccatc ggcaaaatac gctttaatcc ccgcaagaat
    19741 agactccgca acttcctgct ggaaagtcgc cgttttcagt ttacgctctt cctcaacgtt
    19801 actgataaac gccgtttcga ccagaatgga gggaatatct ggtgccttta gtacggcaaa
    19861 cccggcctgt tcaacttgat ttttatgcag cttgttgatt tttccgagct tattcagtac
    19921 cgctttacca aacttaaggc tgtcagcaat agtcagcgac tgcaccatat cgaacatggt
    19981 gtggtcgaca tagcggtcac cgcttttgct cacgccacca atcaagtccg aggcgttctg
    20041 ggtttgtgcc agatattttg ccgcagtact ggttgcgcct ttggttgaga gcgcaaacac
    20101 cgaggaaccg ctcggctgac gactggtaaa ggcgtcggca tggatagaga caaacaggtc
    20161 agcacgctgt ttctgggctt ttgctacgcg cacttgcaac ggaatgaaga tgtcttcatt
    20221 gcgcgtcatg tacaccttca tattgccctc ttcctcgatc agagagcgca aacggcgagc
    20281 tatttgcaat accacatctt tttcgcgcgt tttgtatttc cccaccgcac cggagtcttc
    20341 gccaccgtgg ccagggtcaa gcataatgac aatcggacga tcgcgccctg ctttacccgg
    20401 ttgtggacca ctttgcgctg gcggcacctg cttttcgagg tcgcctttgt tgtaatcctc
    20461 cagcagcgcc agcagcgggt cctgcatatc ctgtgcattg gccggataga ggtccatcac
    20521 cagacgctct ttaaacccgg cgaccggcgc aagggcaaac agctgcggtt ttacgttttg
    20581 ctttaattca aaaaccatgc gtacggtttg cgggtcaaat tgcccgacgc gcgccgactt
    20641 gatgaacggg tcgtctgcac gaatttgtgc cgccatcccc ttgagtaccg agttcaggtt
    20701 tacatcttcg atatccacca ccacgcgttc agggttactc aacgcgaact gcttatattt
    20761 cagctgacga tttgattcta ccgtcacgcg ggtgtagctg gacgcaggcc agacgcgcac
    20821 cgccacgacc tggctaaccg cagccaggct gacctgactt acgctcaata gccacatagc
    20881 gcccgcgcct tgcagtaaac gacgacggct gattgcagtg ttggatcctg acatgcctct
    20941 cccgagcaaa agaaatctaa gctgtgtaac aagtaaacga ctaatttgac cggaaacttt
    21001 agcgaaagac gcataatctg tcatctaata aacggtaaac attcttttta tattcacggc
    21061 attactgata aaaaagtcgc tctcgcataa aatttacact tgcaccctgc gaaaaaacag
    21121 aataaaaata caataatttc aaataatcat gcaaaggggt gtgccgtggt aaaggaacgt
    21181 aaaaccgagc tggtcgaggg attccgccat tcggttccct atatcaatac ccaccgggga
    21241 aaaacgtttg tcatcatgct cggcggtgaa gccattgagc atgagaattt ctccagtatc
    21301 gttaatgata tcgggttgtt gcacagcctc ggcatccgtc tggtggtggt ctatggcgca
    21361 cgtccgcaga tcgacgcaaa tctggctgcg catcaccacg aaccgctgta tcacaagaat
    21421 atacgtgtga ccgacgccaa aacactggaa ctggtgaagc aggctgcggg aacattgcaa
    21481 ctggatatta ctgctcgcct gtcaatgagt ctcaataaca cgccgctgca gggcgcgcat
    21541 atcaacgtcg ttagtggcaa ttttattatt gcccagccgc tgggcgtcga tgacggcgtg
    21601 gattactgcc atagcgggcg tatccggcgg attgatgaag acgcgctcca tcgtcaactg
    21661 gaaagcggtg caatagtgct aatggggccg gtcgctgttt cagtcactgg cgagagcttt
    21721 aatctgacct cggaagagat tgccactcaa ctagccatca aactgaaagc tgaaaagatg
    21781 attggttttt gctcttccca gggcgttact aatgacgacg gtgatattgt ctccgaactt
    21841 ttccctaacg aagcgcaagc acgggtagaa gcccaggaag agaaaggcga ttacaactcc
    21901 ggtacggtgc gctttttgcg tggcgcagtg aaagcctgcc gcagcggcgt gcgccgctgt
    21961 catttaatca gttatcagga agatggcgcg ctgttgcaag agttgttctc acgcgacggt
    22021 atcggtacgc agattgtgat ggaaagcgcc gagcagattc gtcgcgcaac aatcaacgat
    22081 attggcggta ttctggagtt gattcgcccg ctggagcagc aaggtattct ggtgcgccgt
    22141 tctcgcgagc agctggagat ggaaatcgac aaattcacca ttattcagcg cgataacacg
    22201 actattgcct gcgccgcgct ctatccattc ccggaagaga agattgggga aatggcctgt
    22261 gtggcagttc acccggatta ccgcagttca tcacggggcg aggttctgct ggaacgcatt
    22321 gccgctcagg cgaagcagag cggcttaagc aaattgtttg tgctgaccac gcgcagtatt
    22381 cactggttcc aggaacgtgg atttaccccg gtggatattg atttactgcc cgagagcaaa
    22441 aagcagttgt acaactacca gcgtaaatcc aaagtgttga tggcggattt agggtaagcg
    22501 atgaaaatca ccggatgcgg caggcgtaac actcgtgcgt cgcatccggc aattacgttt
    22561 attcccgcga actaaacaac gccgccagac cactgcgccg ctcagtacga gtggcgattg
    22621 ccgcacttaa tatacgctca tcggcataca gcgacagacg acgacgcgcg cgggtcaccg
    22681 cggtgtatac cagctctcgc gttactaccg gcgtgcgttg gctcggtaaa atcaacgccg
    22741 catggtcgaa ctccgatccc tgcgatttat gtaccgtcat cgcccacgta gtttcgtgct
    22801 ctggcaagcg actcggttgc acagacttaa tattgccgtc cggcatcgca aaccagacgc
    22861 gcgtcccctg cccgcgatcc agcgcaatac cgatatcgcc attaaacaac ccaagcgcgc
    22921 tgtcattacg ggcaatcatc accggtcggc cttcgtacca acgagagtgc ggatgacgat
    22981 gaattttgcg cttctgttgc ataaactgtt caattcgctc attcagtcca gccacgccaa
    23041 acggcccttc ccgcagggcg cacaaaagct ggtactcatt gaacgcctga atgattaaat
    23101 ccggctcggc acgcgcttgc agcagatcca gataacgtcc gtaacccgca agagcttcct
    23161 caagcatcgc aatataatct tcgccgctct gtaaaagtcg tttttcgata tcagtaaaat
    23221 cctgctgaaa aacggttttc actgccgttt tatcaccacg gttgatcgcc gcagctaact
    23281 gaccaatgcc agaatcgctg ccgaaacgat agcttttttg cagcaggcag agactgtcgc
    23341 gcaaagatgc cgcttctgtg ccggttcctg ccggaacgtg agtccccgtc aggcggctta
    23401 gctgcctggc acgctcggcg gtaaagcccg cgttggcata agcgcagata tcgcccagca
    23461 cagccccagc ctcaaccgag gccagttgat cacgatcgcc gagaaagatc actcgcgcat
    23521 gatctggcaa ggcgtcgatc agtctcgaca tcataggcag atcgatcatt gacgcttcat
    23581 ctaccaccag cacatcaaga tgcagcgggt taccggcatg atgacgtaaa cgctggctac
    23641 ccggctgcgc gcccagcaat cggtgcaaag tgctggcatc ttccggaatg cgtttctttt
    23701 gttcatcggt cagcggtaac tgtcgcaaag ccttgccgag agattcggtt aagcgcgcgg
    23761 cagctttacc cgttggtgca gccagacgga tacggcagcg ttcgccgtcg gccatttgaa
    23821 ttaacgctgc cagcaacttc gccacggtgg tcgttttacc ggtgcctggg ccgccggaaa
    23881 tcaccgagat ccgccgcgtc agcgccactg ccgctgcaac tttttgccag ttaatttcat
    23941 cgcttactgg aaaaagtttg tccagggttt gcgccagtag agcttcatca acctcaatgg
    24001 tatgattcac ctcattgaag aagcgcgcta ccgtccgctc gttacaccac atgcgattca
    24061 aataaagacg atcgccacag aggatcatcg gcgtgggttc atctcccctg ctgaccgctt
    24121 gagaagccag caagcattct tcccaatttt gtagctcacc gatttcactg acacaggtcg
    24181 ccaacagcgg attcgacgcc tcgttatttt ccagtcgtga aagcggcaaa cagacgtgtc
    24241 cctctccggc atcatgactt aacagtgccg ccgcgagggt gacggcagga tgttcatctc
    24301 ccgccacggt cagggcaaat tgcacatcca gcgggcgtag ctgtttgtgc tccacagctt
    24361 ccagtaattg cttttgcaat ttcattacgc ctcctccagg gtcataccgg caaacatctc
    24421 atccatcagg gcaatcaacc cggcgttggg tcgggttgtg taaatccctt gttgcggatg
    24481 ttctttatca acgccacgca ggaacagata gataacgccg ccaaagtgaa gatcatagtc
    24541 gtaatcagca atgcgatggc gcagataacg atgcagcgcc agggtataaa gctgatattg
    24601 cagatcatag cggtgtgcct gcatcgctgc tgccatagcc tgttgggtgt aagccgaact
    24661 gtcttcaccc agccagttgg atttatagtc gagcaggtaa taacgccctt cgtggcggaa
    24721 taccaggtcg ataaagcctt ttaacatgcc gcgtacctgc atgaactcca gcggcgggca
    24781 gccagcggat agcgggtcaa actggcgaat tagtgcatca agctggctgg cgatgagcgg
    24841 ttcgctaatc ggcaggtaaa actccatctc cacctgttta tcgcggtcgg aaagctgact
    24901 caggctaaca cccgtttcat tgagaggtgc ctggaggaca gccgtgatcc actcggtcaa
    24961 caccggttcc cactgcgatt caaagccgcc gagttccagt ttttcctgta cccagttcgg
    25021 gtcaaccggc tgggtaaaat cgaggtcttc aaacaaactg tgcaagaatg tccccggtga
    25081 cgcaccgcgc gggaactgat gcggtgttaa cgtcggttct tcaacgacgc tgaccacgcc
    25141 tgcggcatca acatccagcc gtggcatcag atcctgagcg ataccgtgac cacgctgctg
    25201 caagccggag tagctggtga cacgccagtt atcgccgggc aatcgttgta gtgtcctcgc
    25261 attcagttct gcagttaaag catcattaac ctgccagggc tggttatcac cagtttgtgc
    25321 cgtttgccag gcaatatcat catcgcataa cgcttcaata caggtgcgaa gccctgccgc
    25381 atcttgcggt tccccttttt gcagcaaacg cccgagcgca ctttggtgga cgtcggtgtc
    25441 acctttttta tcgccacgac ggcgcaccag cggtgcaacg ccgagactgc aatgccaaac
    25501 cgaacgcgtc agcgccacgt aaagcaaacg cagatcttcc gccagacgtt cgacctccgc
    25561 gaggtcgacg ctttctggcg cagcattaag atccaaaact gcctcaaacg agtggcgatc
    25621 gtgataaaac gcctgatcct ggacgcggaa attggtaata aatggcaacc agaccagcgg
    25681 atattccagc cctttcgatt tatggatcgt gacaatctgc accagatgtt tatcactttc
    25741 gaggcgcatt tgttggctgg aggcattact gtctggctcg aggatatgtt gcgataacca
    25801 gcgtaccagc gcatgttcac tttccagctg cgttccggct tcttgaagca gttcgctgat
    25861 atgcaagata tcggtaagac gccgctcacc gcctgccgtt gccagcaagt tttctgcaat
    25921 gttacgcgcc gacatcagcg cccgcagcat cggcataacg ccacgtttgc gccagatttg
    25981 ccgataacca tcgaactctt cgactaccgc atcccacgca tgttcgtcat tattcagcgt
    26041 ttcaatatcc agcgcattca gccccatcat tgacgttgcc agcgcactgc gcagggtgtt
    26101 ctcacgttcg ggcgtcatca ccgcctgcaa caaccaaagc atttcctgcg cttccagagt
    26161 ttcaaaaaca ctgtcgcggt tcgaaaggta aacggaaggg atttccagca acgttaaggc
    26221 atcgcgcacc tgggcggcct cctggcggct gcgcaccagc acgctgatgt ccgaagcacg
    26281 caccggacgc gcgtcgtcgc cgttcatcag caacgcttcg ccccgctgtc cggcttgtag
    26341 ccagtcgcgg atttgcgcag cacatacctg cgccatggta ctttgatagt cgccaacgcc
    26401 gcagctttcg ccttccatca gccacatttt catcgcaggc tgcgtttcac ctttaaatac
    26461 aaaacgtaac gcctgatttt tcccggctga tttcactgga ataaacggta tttcgcgaaa
    26521 catgaacgtg tcatcagtct ggctgaaaag cttgttcaca ctgttcacca ttccaggtgc
    26581 ggagcgccag ttggtgtcta aagtgtagtg ggcatgaact tcactacgcg ccttcatata
    26641 agtgaagata tccgcacccc ggaatgcata tatggcctgc ttcgggtcgc caatcagcaa
    26701 caatgcggtt tccggctgat ggtgccagat acggcgaaaa attcggtact gctgggggtc
    26761 ggtatcctga aattcatcga tcattgccac cgggaatcgc gtacggatcg ccgctgccaa
    26821 cacctcgccg ctttcgctac gcagcgcgga atcgagccga cttaacatgt catcaaaacc
    26881 caattcgcca cggcggcgtt tttcacgcgc tactgtttcg cggatctcag ccaatgcgcg
    26941 ggtgatcaac agatcgcgga tcgacaatgg ttctgcaagc agttgatcga tcgcctcaaa
    27001 cagtggatgt cgcggggttt cccccccggc cttcgtgcga tcttctaaga aacgctggga
    27061 gaatttttcc agcgactccg gcaactgata actgtttgtc tcttcttctg cccaggcgct
    27121 gatcttttcg atccatttag cctgattgct acggttaaac ttgcgtcgat caataccaga
    27181 agattcgatc agcgcatcca gttcacccac tgcgtcgcgc cactgctgtt ttaccgtatc
    27241 aatacgcgcc acaatttgcg cgtggcggga agccagcgtt tcatcatcgg gcggcggtgc
    27301 tttgataacc ggcggttcgc cttgcagata acgattaata tcgcgcagca acgcctgcgg
    27361 ccctttccag gtttcaaaga cgacctgggc tatttcacgc ggcagcgggt agcagtggcg
    27421 acgccagaaa tcggcgcagg cctggtagcg tagcagagac tcatcttcaa tcagctgctg
    27481 ctcaaacagc atgccggatt caaaggcatt caggttgagc atgcgctggc aaaagccgtg
    27541 aatagtaaag actgccgctt catccatctg ccgttcggct aacaacaacc actgcgcggc
    27601 ttgcgcttta tcgtcgatct cttccagcag gcgttcgtac agtggattgt cggtggtttc
    27661 acgcagacag gcgatgcgca actcgtggat attgctacgg atacgaccgc gcaattctgc
    27721 cgtggcagcc tcggtaaagg tgaccaccag cagttcttca acgatcagcg ggcggggaaa
    27781 ggcggcggaa ccgcctagtc caagtaacag gcgcaaatag agcgccgcaa tcgtaaaggt
    27841 tttgcctgtg ccggcagagg cttcaatcag gcgctcgccc tgtaagggca agcgcaaagg
    27901 atctagtgtc tcggcgacat cactcattct tttcactcat caggggcatt gtttgctgca
    27961 acgcgctgac gttctcccac actttccagc cttcaggatg cacatattcg gctttcccgt
    28021 tctggctgcc ggaaatctgc gacagaatag ccatgccttg cggctcgacc accgcctgat
    28081 ggaagaaatc agcaagtttt tgcggcgtca gcagttttat ctgggccacg attttatcac
    28141 gcgaatcgaa gcgcatattg ccgcgatcga aatctttact taacttcgat gcttcttcgc
    28201 cgagcgtttg cggtgcctgc agcatctggg taattaccgc ctgctggatt tgcgcaaact
    28261 catctggctt catcgctcgc aattttgcct ctgcggttgg gaaaaacgcc ttgtaacgct
    28321 cccacaagaa tgaaggctgt ttatcattgc tttgcaaaag gaagcccatg ccccactgac
    28381 gccccacgct cattggaaac gcaaacacgg catagcccaa ttgttcttcg gtacgcaact
    28441 gattgtagaa ccacggctgt acgatctgcc ccaacagaga gctataggct gagctggtgt
    28501 attcatcgta gccagtcggt acaaataccg ctgccagtgc ggagtcggtg ctgttaccgg
    28561 ctttttcaaa gatgacggat tgttttttat cgaccactac atctttgttt cgacaccact
    28621 ctgaaccatc agcgcccaac tgtttttgca catcgcgtgc cagcgttgtt gcctgggcct
    28681 cggtcatgtt gccgataacc ataaactctg gtcgagcccc tgattttaag gcgtcgcgat
    28741 aggccagcac ctctttcaac gtaatggagg gcaaaatttt acgccgttca tctcgcgaga
    28801 agtacggcac ttgcgagagc atctgcgcgg gcataatcgc ctgctcaaac gctttaccct
    28861 tttctgcgga atccatcatc tggttatacc aggacttcgc ctgctcaagc tgctcttccg
    28921 tagcggtata gctaaagtac ccctcgagca atgcctggaa cagctgcggc agacgctggg
    28981 tgtaaccatt agcattaatc ataaggccgt tgttagcgtt ggtggaaaaa cttatgccac
    29041 caaccgacgc ctggttgctt aactgatcaa gcgccagccc tgcgagataa tcattgagcg
    29101 caaacatcac ctgattgcgg gcgctgtcca tggctttcgg attacgcaaa atcaggctga
    29161 catcagcttt gggctcgctg gcaaaataac ggcttggcgc atacaccacg cgcagattcg
    29221 actcatcaac aatcagctct ggatggtcgt atttcttctc tgacttaatc agcgagaaat
    29281 catcaggaat ataagggtta agctctggca aagagagcgc aatgtcggcg gcttttttct
    29341 gccagtcggc gaaagtttgt gcgctgattt tatcgacctg atacggcgca tcgacaaagt
    29401 aagccgtttt gttgtgcggc tctttcgggc tgatatacca gatacgcgca ttctgcggcg
    29461 tcatcatcgc cagacgttcc tttactgctt tagcatcgta ccgatcggca atattgactg
    29521 catccagcgt atgctcaaca ggaacgcgaa tcatggtatc tgccagccat tcgacgtaat
    29581 ccatatcacg ggtgatcgac ggataacgga agtcgatatc cagcacattc gccagttcat
    29641 cgaagtattg tttatcaatg cctttttcac gtaacagatt gagatagcta aaaattgccg
    29701 ccacaacctg atcgcgatta gccaggcctt tatcagttaa agacgcagag atcgctaata
    29761 cgccgctgtt gccgttgacg ataggatcgg agttggcgct aatgccctca actaatccct
    29821 gcttttgcag ccagtcagaa agtgtacctg ggctgcgatt gccaatcaga taggtaatca
    29881 attcatcggt tttactacgg aacttcgctg agttgttatc gatgcgaaac tcaacgcgca
    29941 acactttacg cggcagcgca gggacgtaat gaatgataat gcccttttgc gcgtcggtga
    30001 ctaccggcac ggtgatttcc ggttttttgc tctctttgtt cggcacgcga ccaaaggtgt
    30061 ccgccgccat ttttgccaac tccggcagcg gtttattact gtaaataacc gccttcatca
    30121 aattggcgga atagtacttc tcgtggaaat ctttcagcgc ctgctgcacc ggattaccag
    30181 gtttgtcgct taaagtttcg aggttaccac cagaaaactt tgaaccgggg tgtgccgggt
    30241 taatggtttc tgcgctgacc tgtgccatgc gcatcccgtc acgcgtacgc gccatggtta
    30301 attcagcgtt cactgcatta cgctcacgtt cggcgtattt cttgtcgagc aagggttctg
    30361 caatagcatc ggccaggcgg tctaccgcac cgggcaaggc gtcgttctca acttccagat
    30421 agaaagccgt gcgatacggc gcagtgctgg cattgtgact accgccgtgc attttgagat
    30481 attcggccag actgtcagcc tgcgggtact ttttcgaccc catcagactc atatgttcaa
    30541 ggtaatgtgc cagcccctgg tacgcctcgg gatcttccag cgacccaacg ggcactacca
    30601 gcgccgagag cgatttaact gcctgcggat cagaaaccag caagaccacc ataccgttat
    30661 ccagacgtat agcctgatac tggcggttat ctttatcact tttacggatg gtttcctgaa
    30721 tcggctgcca tcccgtttct gcctgactta atggtgccca gagggcaaca aacaacaata
    30781 atgctttgaa ccaggtgctg cggggcattc acggacctca taatcaactt tattttctgg
    30841 acagattcaa caggttaacg caaatcatct gccagaattt aatcttgtgc tgcacgagcc
    30901 agcctatgtt tatataacca tcagtccgtg actggtgcgc atcataaagt aagcagatag
    30961 attgcgcaat ttttatacaa cactcatgac tgattaaagc gaaacagcgg taacaggaaa
    31021 cgttgcgact gttcaacgat ggcctccatt gtctctggtg ttaattgccg ccagagccgt
    31081 tgataccaga tatcatcacc ttcgccacgc accatcatgt tgccttcgta agcctgaagg
    31141 aatttcgtac gggctttttg caacgtggaa tcgtcatcca gcatggcatc gttttgcgcg
    31201 tcataacagg tttttagcca cgcgccgcca ctttcaggta acaccagcaa tggcgcggac
    31261 attccttcac gatacccctc aatcagttgt gagaggtaat gcaaagcctg ttcggctgca
    31321 agcggcggaa aacgccactc accgtctttg cgtagaaaaa ggcgactttc gccattacca
    31381 ccgctggcac agtagacaag gtgttccagc caaagttgca ttccctgcgc cacacttaat
    31441 aaagagggac gccagcgcaa caggccatcc ggctgcacct gcggcaacca gccagttatc
    31501 tgcacaccgt tgcaggcgag atcaatttcc atactctgcc ccggctggcg acaggcaatg
    31561 actctgtcgg caagctgctg catctcctgg cactgtgttt cccagaaaat ttcaccaaaa
    31621 gcgccatacg gtaaatcccc tgccgcccgg aagcggcgga acaagcgttc ggcatcatcc
    31681 tgctcaacca gtgcattcaa taactgctga ttgatttgat aacggctaag tccttccaga
    31741 ataaatggct cggtgtctgg gatttcgctg tcttcagtat ggaagttcac ctgcaaacgc
    31801 atctggaaga acgcccgcac cggatgtgcc cagaatcgtt gtagcgtttc cagcggcacg
    31861 gtttccggta aggtaaacgg cagcggctga acaaattcag aatgtgcttt accagcctgg
    31921 ctggccgcag gtagccattc acgagcatag ctttgtcgtt cgcctggctg gtagttttgc
    31981 ggatcaaacg gcatccgggt atggaggcaa gtaagatgcg cttttaccct tgcctcgctt
    32041 tcatcacagt tgagcgcttc atcgcccggt agataatgac tttgcccgat gtagtcgatc
    32101 agttcctgca ccagtaccga cgggaaacgt tcactgttat cctgaatgga acgcccgata
    32161 tagctgatat agagtttttg ctgcgcggaa attaacgctt ccaggaacag gtagcggtcg
    32221 tcatcgcgac ggctacggtc gccacgcttc ggtttctggc tcatcaggtc aaagcccaat
    32281 ggcgcaagct gacgtggata aacgccgtcg ttcattccca gcaggcaaac cactttgaac
    32341 ggaattgaac gcattggcat cagagtacaa atgttaaccg gtccggcgag aaaacgctgg
    32401 ctgatacgtt cttgatccag acgctgtgcc agttcatcac gcaatagtga cagcggcacc
    32461 gcgtcgccat actgcgcacc taaaccttcg gcgataatcg cctgccattg ttgttcgatc
    32521 agcgtcatcg ccgcttcggt ttccgcatcc ggcaggaaga aggcgttgag catatcgcga
    32581 caaaccggca accactcttc tagcggacgc tcctgtgcca gcccgcgacg ccagatgttt
    32641 agctgcatta gcagtgaagc cagatgcccc accagttctg caattaagcc gctcgattca
    32701 tcataaggta gaaccgattg ccactcgccc tgcgcgctct ccatcgcgta gcccagcaac
    32761 atacgcgtca ggccaaatcg ccaggtgtgt tgtccggtgg cggggagttc cagctcgcga
    32821 acgttgtcgt catctatccc ccaacgaatg ccggattcgt tgacccactg gcgtaaataa
    32881 cgcagccctt cttcggtgat gtcaaaccgc gccgccagca ccggcacatc cagcagcgcc
    32941 agcacatcct ctgacacaaa gcgactgtca ggcagtgata acaggctgat aaacgcttcc
    33001 agtaccggat gtgactgccg cgcacgacgg tcggaaatgg cgtaaggcag gtaacgatcc
    33061 gcaggtgcgc taccaaacac tgcctgaata aacggactgt agctgtcgat atcagccacc
    33121 atcacgatga tgtcgcgcgg agtaagtgtc ggggcttcct ccagcatcgc cagcaagcga
    33181 tcgtgtaaaa cttcaacttc acgctgcggg ctatggcaaa cgtggaatgt gatactgcta
    33241 tccagtggat caagcgggcg tttgttatcg ctacgggaaa actcttcgat gttcacaccc
    33301 gcaacggcgc ggttttccag ttccagaatg tcagactgaa tgttatgcag caggttatct
    33361 ggcgtcacat cgacaaaagc gtccagctcc tggctgctct ccaggtcaga aaggagataa
    33421 atgtagtcgc gcccaagctt cccccatgag gccagcagcg agttgccgac atcctgttca
    33481 ccatcgctgt taaagagctg tccggcattt tcgctatcgc gaaatagcgg caattcgcga
    33541 tcttcaaaac tgtgtcgacg ctgacgagtc agcagtttcg ccagataagc aggatcttta
    33601 atatcgcccc agtaataacg gcaggggttg gtaaacagga gatggatttc aatatgttta
    33661 cccagcgcct ggagcgcctg gagataaaca ggcggtaacg cggaaatacc gcatataaag
    33721 acgcgcgaag gtaaccccgg cgggcaggtc gtcgcggact ccagcgtttc gataaagcgc
    33781 tgatagagat tcgcgcggtg ccagcgcggt tgtccgagtt catgggtata ttccaccagc
    33841 gccttccaca acggggcttg ccaggcctgt gcttctccca gcccttcaac caaatgtcct
    33901 gtttcccact gtgccagcca gtccggacga tagaccagat actggtcaaa caggtccgcc
    33961 gcttttgagg aaagctggaa cagttttcgc ttgtcgctat cgtcagtcag ataatgccgc
    34021 aacagggtaa agtcttcgcg ctccagcaat tgcggcagca gagtcatcag tttccagctc
    34081 atgctctgtt tgttaaaggc gctctctttg gggatttccg gtaacacccg gacgaacata
    34141 tcccagataa agctcgctgg cagcggaaaa tcaatgtttg ccgcaatacc aaacttttgc
    34201 gacagggtca tttgcagcca ctgcgccata ccggtacttt gcaccagaat catctctggt
    34261 tcgaaaggat cgtccagccg ttcgcgttcg acaataaact ccatcaacgc ttccagcacg
    34321 tccagacgat tggaatggta gacccttaac atagcggctc ctgactactg acaattcggg
    34381 caatgcaggc gggtcatctc gccttctctg cccccgggtg aaactagcgt aacgctgatg
    34441 ctgacacatc ccgcctgcga tgtctgcatt cggttgacct gccagttggc aggtggcgaa
    34501 atcgcgcgca gttgcgtttg ctgccaacca tgccgccaga gcttttggta ctggtttctg
    34561 ctggcgagac tgttcattga tgttcgctgg atacccgata acgcagtgac aatcatcacc
    34621 atcagcacca tcagcaccat cagcaccatc agcaccatcg caaccattac ctccggcagg
    34681 ctaaagcctt gttgattctt cagggaagct gacataatgc cccctctttc agcggacaaa
    34741 aatcgctcca gccgcgtggc gaaaaaacaa tttttccatc aatgacttcg cctgttcgcc
    34801 acaacgaaac gccttcataa ccggcaatca ataaggcttc attatctgcc agtaaacgca
    34861 aacaaacctg ggcatcggtt ccagcgtact gcgagcactg aactgctggc tgcgtctgcc
    34921 aggagtgcat ttttccccac gccagcgccg actgaacgat ggcctggcgg cgcaatgact
    34981 gactttccat gctcacgcga gaggcaaaac tgcgatcctg ctgactcatt ccttgcaata
    35041 gcaagctacc caaaaccagc agcatcagga ccagagccag tgacgaaaca cctttttcgc
    35101 ggttcacagg ttgaatcctg tcacgctata gctggcatcc accacggttt gcggttcaga
    35161 cttactggca gcacgcatat taaccgtcaa caccggcgag aagccgctga catcctgacg
    35221 tacgacctga aaagtgtcga taatgatggc atccggatta gtgactttat cccagccctt
    35281 accttcacag gatgtcgcac cgcgtagcgt ttccagcaca tgctccttca gacgaaatcc
    35341 aatctggtcg gactctttta ccggttcgcg atcccagata ccgttactgt tcgcatccca
    35401 ctgcacaatg atacagtcac cctgtccgac aatttccagc ccttcgccgg tacagctgcc
    35461 atgacaataa cccgccctct ggagatgctt cgcgacggta aatacccgca gccagatttc
    35521 atcttccagt gccagcttac gagtgctcgt taaactttca cgctgtaacg caggcagaaa
    35581 gcgtgcagcc cccagcaaca atacgctact gatcgccata gcaatcaaca cttccagcag
    35641 agaaaaacct tgctctttta caggcatcct tctgtttctc cttgctgata aagccggagt
    35701 cttccccacg gcgaaaccac cagccaccac tcgcccgttg agtttttgaa gcgaatatgc
    35761 acggcccatg cggtattgcg caggccaaag aaagcaagcg aaggtgtcag gtcgctcatt
    35821 tcgacttcgg gccagcgtgg cacaaagacc aatggtgaac tgccatgaca ggtattggcc
    35881 ccagcagcgg aactcacaag gcaccataac gtcccctccc tgataacgct gatactgtgg
    35941 tcgcggttat gccagttggc atcttcacgt aaatagagca aatagtcccg cgcctagctg
    36001 gcggtttgcc aaagccgttg cgactgctgc cagtattgcc agccatagag tccacttgcg
    36061 cttagcatga ccaaaatcag catcgcgacc agcgtttcaa tcagcgtata accacgttgt
    36121 gttttcatgc cggcagtatg gagcgaggag aaaaaaagac gagggccagt ttctatttct
    36181 tcggcgcatc ttccggacta tttacgccgt tgcaggacgt tgcaaaattt cgggaaggcg
    36241 tctcgaagaa tttaacggag ggtaaaaaaa ccgacgcaca ctggcgtcgg ctctggcagg
    36301 atgtttcgta attagatagc caccggcgct ttaacgcccg gatgtggatc gtagccttca
    36361 atctcaaagt cttcgaaacg gtagtcgaag atggattcgg gtttacgttt gataatcaac
    36421 ttcggcagcg gacgcggttc gcggcttaat tgcagatgag tttgatccat atggttgctg
    36481 tacaggtgcg tatcgccacc ggtccagaca aaatcaccca cttccagatc gcactgctgc
    36541 gccatcatat gcaccagtaa cgcgtagctg gcaatgttga acggcaggcc gaggaagacg
    36601 tcacaggagc gctgataaag ctggcaagag agtttgccgt ctgccacata gaactggaag
    36661 aatgcatggc acggtgccag cgccatttta tccagttcgc ctacgttcca cgctgaaaca
    36721 ataatgcggc gcgaatccgg gtcgtttttc agctggttca gtaccgtagt aatctggtca
    36781 atatgacgac catctggcgt tggccaggcg cgccactgtt taccatacac tggcccgagg
    36841 tcgccatttt catcggccca ttcgtcccag atggtgacat tgttttcgtg tagataagca
    36901 atgttagtat cgccctgcag gaaccacagc agttcgtgga tgatggaacg caggtggcaa
    36961 cgtttggttg tcaccagcgg gaatccatcc tgcaggttaa aacgcatctg atgaccaaaa
    37021 atggaaagcg ttccggttcc ggtacggtcg tttttctgtg tgccttcgtc gagcactttt
    37081 tgcatcagtt ctaaatactg tttcatggtt cctcaggaaa cgtgttgctg tgggctgcga
    37141 cgatatgccc agaccatcat gatcacaccc gcgacaatca tcgggatgga aagaatttgc
    37201 cccatgctga tgtactgcac ccaggcaccg gtaaactgcg cgtcgggctg gcggaaaaac
    37261 tcaacaatga tgcgaaacgc gccgtagcca atcaggaaca agcctgagac agctcccatt
    37321 gggcgcggtt tacgaatata caggttgagg ataataaaca gcaccacacc ttccagcagc
    37381 agttcgtaaa gctgtgatgg gtggcgcggc agcacaccgt aagtgtcgaa aatggattgc
    37441 cactgcgggt tggtttgcag cagcaaaata tcttctgtac gggagcctgg gaacagcatg
    37501 gcaaacggga agttcgggtc aacgcggccc cacaattcac cgttaataaa gttgcccaga
    37561 cgcccggcac caagaccaaa cggaatgagt ggtgcgataa aatcagagac ctggaagaag
    37621 gaacgtttag tacggcgggc gaagataatc atcaccacga taacgccaat caggccgccg
    37681 tggaaagaca tgccgccgtc ccagacacgg aacagataca gcggatcggc cataaactgc
    37741 gggaaattgt agaacagaac ataaccaata cgtcccccga ggaagacgcc gaggaagccc
    37801 gcatagagta agttttcaac ttcatttttg gtccagccac tgcccggacg attcgcccga
    37861 cgtgttgcca gccacattgc aaaaatgaac cccaccagat acatcaggcc gtaccagtga
    37921 agcgccacgg gtcctattga gaaaatgacc ggatcaaact ccggaaaatg cagatagcta
    37981 ctggtcatct gtcaccacaa gttcttgtta tttcgctgaa agagaacagc gattgaaatg
    38041 cgcgccgcag gtttcaggcg ctccaaaggt gcgaataata gcacaagggg acctggctgg
    38101 ttgccggata ccgttaaaag atatgtatat gatccgcgct ataaccctcc gcgaatcagc
    38161 ccgcccatgc cgcgacgctc cataaaggct gcaacctgat gacgaacttc ggtcgccagt
    38221 tgcgcttcca gactacgctg cgcaagattt tctgcttcgg caaaatcaat gcgccgcagc
    38281 aggtatttta cccgcgctac agaacgtccg ttcatagaca gatggcgata cccaagccca
    38341 atgaggattg ccacgcacat gggatcgccc gccatttcac cgcacaaacg aagatcgata
    38401 ccatgtattt ccgcttcccg ggcgatcatc gccagagctc gtaacattgc aggatgaaga
    38461 ctgtcgtaaa tgttcgccac ccgggtattg ttgcgatcaa cggccagaat gtattgagtc
    38521 agatcgttgg tgccaacaga gatgaaatcg accctctttg ccagatgcgg cagcataaat
    38581 accattgacg gcacttccag catgatgccg atacgtggtt tgggaatttc gtaaccgatc
    38641 atctcctcga cttcacgtcc ggcacgttca atcaggcggc gtgcttcgtc aacttcatcg
    38701 aggctggtga ccatcggcaa cagaatattc aggttgcccg tagcggcatt agcacgcagc
    38761 atcgcccgca cctggatcaa gaagatctcc ggctgatcga gcgtaatgcg aatcccacgc
    38821 caacccaggc atggattctc ttcgctgatc ggcatgtaag gcagctgctt atctgctccg
    38881 acatccagcg tacgcaaggt gacgggttta tcattgaaca tttgcagcat cccctgatac
    38941 tgcgccacct gttcttcttc cgacgggaaa ccactttgca gcatgaatgg gatttcagtg
    39001 cgataaagac cgatgccatc aatacagctg cccagttttt cttcatgttc cgggcttaaa
    39061 ccagcattga gcatgacttt tatacgctca ccgcttttta actgcgcggg taaattgacg
    39121 tcatcttccg ccagacggct aagctcaatc tcttcactaa ttagccgctg atattcttgc
    39181 agcagtaccg gctccggatc gaccagcaat tcaccgcgat agccatcaac gatcagcgtc
    39241 ctacgatgca gcaccgaagg ctgaatatcc gcgcccatca cggtagggat ccccagcgca
    39301 cgtaccatga tcgcagcatg ggagttggct gcgccatctc gcacgacaac accgactaag
    39361 cgatcctggg gcagctcagc aagagttgtc gctgacagtt catctgccac cagaatgaaa
    39421 cgttccggcc aggcgttcgg cccttgatta gcgtcatcaa gatgaaacag caatcgctga
    39481 cccagcgcac gtaaatcacc agcccgctct ttgagatagt tatcgcttag cgcggcaaac
    39541 tgttcggcaa atttttcaat gaccgttttt accgcccact ctgccaccga gcctttatca
    39601 acctcggcaa acaattcgcg acgcagccgg gtgtccgaaa gcaggtgcga gtaaagatcg
    39661 aaaatagccg ccgtttcttt ttgtgctccg gcggcaaagc gtttgctgta gcggcgaaac
    39721 tcgtttgccg cttcttccag cgccccggtc agtcgttcgc gttccagagc cggatccagc
    39781 gttgatgcct gatacacctg ttccattaaa ggtaacgtgg catcctgcca gccttcggca
    39841 atcgccacac caggtgctgc cggtaatgcg cggattcgcg tctggcgata ttgcccaaac
    39901 agggcagtca actgcgactg agaaagaata gctgccatct gggtggcaag cgtcaccagg
    39961 aaggattctt cactttcgtc atactggcgc aactctcgtt gctgtaccac cagtacacca
    40021 agcaactggc gacgttgaat aattggtacg cctaaaaacg cgcggaaacg ttcttctttt
    40081 acggagggga tgtatttgaa gctggggtgc ttttgcgcat ctgcaaggtt tatcggttcc
    40141 gccagcctgc caaccaggcc gacgatccct tcatcaaacg cgagcgttac agtgcgaccg
    40201 cgtggttttt tcagcccccg ggtcgccatc aggtagtaac aacgtcgatc atgatcggcc
    40261 aggtagaccg aacagacctc ggtatccatc gcaagacaga tgtcggtaac cagaatattt
    40321 aacgcctcat tcaggcgtgg tgcgctggct accttttcga ctatttcgcg caggcgagtg
    40381 agcataattg gcgtgactta acctctttta cgtcgataag cagatgcgtt ttgtggtttt
    40441 ggcgtatttt cctgcagtga catcaccaca ctcgcgaact ctttcattac cctacggtag
    40501 acatcacgtt taaatgacac cacctgtctg accggatacc agtaacttac ccatcgccag
    40561 ccgtcaaact ctggtgtact gctggtttgc atattgattt ctgcatcgcc gcttaccagc
    40621 tgcaagagaa accatttttg tttttggccg atacaaaccg gcttcgtgtc ccaacgcacc
    40681 aaacgtttcg gtaatttgta gcgcaaccag ttacgcgttg aagcaaggat tcgaacgtct
    40741 ttgcggctta atcctacttc ttcaaacaat tcacggtaca tcgcctgctc tgcggattct
    40801 ccggggttga ttccgccttg cggaaattgc caggagtgct gaccaaatcg ccgggcccac
    40861 attacctgcc cctggcgatt acaaatcacg atacctacgt ttgggcggta gccatcgtca
    40921 tcaatcaccg gactacctca aaataaagct ttatatacga atgattgttt catactccag
    40981 gaagacggta aaccactctc tgcagggcat tacacactaa taacaattga ataactcaca
    41041 gttatgtgca gagttataaa cagaggaagg ggtggatagc cgtttttatt caccttttct
    41101 gtggatagag ttgtgaagaa ctacggaatt actacgggaa aacccggagc attctgaata
    41161 agcccgatat agacatttta aaatattcat tattagtcaa tgcattacat gtaatttgag
    41221 taatgaaaaa gagtaattcg tgacccaggt cacacctctc atttacgggt tggcgaaaga
    41281 tcaaccaatg ccgtgtttat ccacagaatg tgccactaag ttaagcactg aaccactaaa
    41341 aactggagtt tcgtcgcacg tcaaggctgt aaatgaaaac agtagtggag gtttttcaca
    41401 gttatcccag ctttctgtgg ataacatggt gtaagatcct gtttattttc agtgaccaga
    41461 tttggaaaac ctattgcagt gttgcgcaac tcgattaccg gcagcctaaa aagcaatata
    41521 aatcagagaa ttgaacaaca catgtggaaa aagttacact gcgaatattc ggcacataat
    41581 tgctgtttgt tttttaacca aggtatcatg acatgtccca acctcgccca ctgctctctc
    41641 ctcccgaaac tgaagaacag ttgttagcgc aagcacagca actttctggt tatacattgg
    41701 gagaactggc ggcacttgac gggctggtta cgccagagaa tttaaaacgc gataagggct
    41761 ggattggcgt gttactggag atctggctag gtgccagcgc agggagtaaa cctgagcaag
    41821 attttgctgc tctgggcgtg gaacttaaaa ctatccctgt ggatagtctt ggtcgtccgc
    41881 tggaaacaac attcgtttgt gttgcccggt taacgggtaa tagcggggtg acctgggaaa
    41941 ccagccatgt gcgccacaag ctcaaacgcg tactgtggat accggttgaa ggcgagcgca
    42001 gcatcccgct ggcgcagcgt cgcgtaggat caccgttgct gtggagcccg aatgaagagg
    42061 aagaccgaca gctacgcgaa gactgggaag aattaatgga tatgattgtt ctcggtcagg
    42121 ttgagcggat caccgctcgt cacggggaat atttacagat aagaccgaaa gcagcgaatg
    42181 cgaaagcgct taccgaagcc attggtgccc ggggcgaacg gattctgacg ctgccacgcg
    42241 gcttttattt gaagaagaat ttcaccagtg cactactggc ccgtcatttt ctgatccagt
    42301 agccatcgct ttgacctgcc gctttccggg catataatta ccgcttcatt tttttggcag
    42361 ggctttttag atgttatttg catggataac cgatcctaac gcttggcttg cgctcggtac
    42421 gctgacgctg ctggagatcg ttcttgggat cgacaatatt attttccttt ctctggtggt
    42481 ggcaaagctt cccacagcac aacgtgctca tgcgcgccgt ctggggttgg cgggagccat
    42541 ggttatgcgt ctggcgctgc tggcatcaat ctcttgggtt acgcgcctga caaatccgct
    42601 ttttacaata ttcagtcagg aaatttctgc ccgtgatttg attctgcttc tgggtggctt
    42661 gttccttatc tggaaagcca gcaaggaaat ccacgaatcc attgaaggtg aagaagaagg
    42721 gctgaaaaca cgcgtttcat cattcctcgg cgctatcgtg cagattatgc tgctggatat
    42781 tatcttcagc ctcgactcgg tgattaccgc tgtgggtctg tcagatcacc tgtttattat
    42841 gatggcagcc gtggtgattg ccgtaggcgt gatgatgttc gccgcgcgct cgattggtga
    42901 ttttgtcgaa cgccatcctt cggtaaaaat gctggcgctc tctttcctga ttctggtggg
    42961 ctttaccctg attctggaaa gtttcgacat ccacgtaccg aaaggttaca tctacttcgc
    43021 gatgttcttc tctattgcgg ttgaaagcct caacttgatt cgcaacaaaa agaatccgct
    43081 ctgatactcc gtacgctctc ctgcgggagg gcgtactcac tccttttacc ctcacagttt
    43141 aagattttcc tgctttcaag atatataacg tcggtttata aacagactat tatcataggt
    43201 aggccaaatg aggataaccg gatgaaaaaa tgggcagtaa taatttctgc agtcggactg
    43261 gcgtttgctg tttccgggtg ttccagtgat tacgtcatgg cgaccaaaga tggccgtatg
    43321 attttgaccg atggaaaacc tgaaattgat gatgataccg ggctggtgag ttatcacgat
    43381 cagcaaggta acgcgatgca aattaaccgt gatgatgttt cgcaaattat tgaacgttaa
    43441 caaataaggt cagcatccgg ctggccttaa gatttttctc tttccctttt cccttccctc
    43501 tgccattttt atattcctta tgtcgtgatt ataaaaagga aacggctatg caatatcacc
    43561 gtatacccca cagttcgctg gaagtcagca cgctggggct tggcacgatg acgtttggtg
    43621 aacagaacag cgaagccgac gcccacgcac aactcgacta tgccgtcgct cagggcatta
    43681 accttatcga cgttgccgaa atgtacccag tacctccgcg ccccgaaacg caagggttaa
    43741 ccgaaaccta cgtcggcaac tggctggcga aacatggcag ccgcgaaaag ttaattatcg
    43801 cctccaaagt gagcggaccg tcgcgcaata atgacaaggg catccgcccg gatcaggcgc
    43861 tggatcggaa gaatatccgc gaagcgctgc atgacagcct caagcgccta cagactgatt
    43921 acctcgatct ttatcaggtg cactggccgc agcgcccgac caactgcttc ggcaaactcg
    43981 gttatagctg gacggattct gcgcctgcgg tttcgctgct ggatacgctg gacgcactgg
    44041 cagagtacca acgcgcggga aaaattcgtt atatcggcgt gtcgaacgaa actgcatttg
    44101 gcgtaatgcg ctacctgcat ctggcggaca aacacgatct gccgcgtatt gtcaccattc
    44161 agaaccctta cagtctgtta aaccgcagtt ttgaagtagg tctggcagaa gtcagccagt
    44221 atgaaggggt cgaactgctg gcctattcgt gcctgggttt cggcacgctg accgggaaat
    44281 atctcaatgg tgcaaaaccc gctggcgcac gtaatacgct ctttagtcgg ttcacccgct
    44341 atagcggtga gcaaacgcaa aaagccgtcg cggcgtatgt tgatatcgcc agacgtcatg
    44401 gccttgatcc tgctcagatg gcgctcgcgt ttgtacgccg tcaaccgttt gttgccagca
    44461 ctctgctggg cgcaaccacg atggatcagc tgaaaactaa catcgaaagt ttgcatctgg
    44521 agttaagcga agacgtatta gctgaaattg aagcggtaca tcaggtttat acttatcctg
    44581 caccataaag attaacgccg gttaaaaccg gcgttaaata ttaatgacgg cgctgccaga
    44641 tccacagcgc cgttattgcc agcgcaaata gcgcaccgaa gccaatgcca atgggcacga
    44701 ccgggatgcc taccattacc gccagcgagt aaatgcccag catcaacaac atggcgctgt
    44761 tttcgccaag gttttgtact gcaatcgcat tccccgcccc gacgcttttt ttaccccgct
    44821 cctgtagcaa cgcattgagc ggaacgacaa aaaagccccc cagcacgcca atcagcatca
    44881 acaaggcata ggctggcagc agctcgtgtt gcagggagaa aatcggtacc accacgccaa
    44941 tcaaaatccc ggctggcata cagcgtgaca cggtttccag cgtaaccagc ttcgctgccg
    45001 cacctgcgcc aaccacgata ccaatcgcta ccatcgcgtt gagataggtc ggcgtagcgt
    45061 tatcggtaat gcccagcgcc accggtaccc acagcaccaa caggaaacgc agcgtgacac
    45121 ccgctcccca gaataaactg gtgcccacca gcgaaaaacg cgtttcacca ttgcaccata
    45181 gcgaggtgca ggcattcagg aaactgcggg tcatgttgat gagattccag gactgccccg
    45241 gacgcgccgc cagtttggga atgtagatat tggcaacgac cgcaccaccg taggccagtg
    45301 cgcatgcggc cagagcgacc aggacatgcc agtcagccag cacaccaccg gctacggaac
    45361 cgagtaaaat cgccgctatg gcagaagctt ccattaaacc gttagctttt actaacttac
    45421 tacccgtggt taattcgccg agaataccgt atttcgccgg tgaataggct gcagcaccaa
    45481 cacccaccag cgtatagccg agaaacggat tgataccaaa gcagatactg gctgcgccca
    45541 gcagcttcag gccgttggca aacatcatca cccggccttt ggcgaagcta tccgccacct
    45601 gcccgacaaa cggcgccaaa agaatgtaag cacctacaaa caccatttgc aggatgggct
    45661 ggctccactc cggatagaac tgcgctttca gtaacgccag agtggcaaac agtagcgcgt
    45721 tatcgccaaa cgcagagaga aactgcgcca cgataaccgc tttcatcccc ttcgaccaca
    45781 acgaagtgtt agtgtgcact gactcactca tcgtgttgtt ccgcttcgtc tacccagctt
    45841 ttcaacgtga caaagtcagg tttgccgctg ccaagtaatg gcatctgttt cagatagcga
    45901 atatcgcgcg gtacagcaag ctccggcacg ccgtgctcgc gggcatactg ttgcaactta
    45961 tcgcgcgtca gttcgttatc tgtggtgaaa agcaccagtg cctcgccttt gctggcatcg
    46021 ctcttaatcg cagtggcatg gactttatct ggcgaaacac caagtgccag ttgttccacc
    46081 atttccagcg acaccatttc gcctgcaatt ttggcaaagc gttttgcgcg gccctgaatc
    46141 tgcacaaagc cctgctcgtc aaaacgcaca atatcgccag tgtcatacca gccgcgctcc
    46201 atttcgccgc gaacattctc ggcggtgggc acttccagta cacctggctt ctccacccgc
    46261 agatagccgt tcattatgtt cggccctttc agttgcaggc gtccgccctc ttcgatacca
    46321 gggaccgaca acaggcgtgc atccattcct ggtagaatac gccctaccgt accgggtttc
    46381 gccgccatcg gtacgttgat agaaacgaca ggcgcgcatt cggtcacacc gtagccttca
    46441 aggatgcgca ggccaaattt atcctgccaa agctgtttgg tactttcttg tagtttttct
    46501 gcgcctgcca ccacatagcg tagacgatag aagtcatacg ggttggcgaa acgcgcgtag
    46561 tgaccgagga aagtcgaggt accgaacaac acggtgcaac tgcggtcata caccaagtcc
    46621 ggcacaatgc ggtaatgcag cgggcttgga taaaggaaca cttctgcacc tgtaagcagt
    46681 ggcgtaaaca ggcctaccgt cagcccaaag gagtgaaaca gcggtaatgc cgacataaag
    46741 cgatcgttgg tggtgaagtc ggcaatcgtt ttaatctgct cgacgttcgc cagaatgctt
    46801 ttatggctat ggacgacgcc ttttggatgg ccttcagaac cggaggtaaa gaggatcagc
    46861 gcctcttctt ccggctgctg ttttacctgc gccaggcgcg gcatcagcaa atgagcgaag
    46921 atccatactt tgtcggcagt ggtgacatct gcttttaaat cttccagata gacccagcgc
    46981 acctgagtaa gttgctccgg cagatgccag agtttgcctt tatcgagaaa ctggcgggaa
    47041 gtgaagatgg ttttgatttc agccgccgta atagcactgg tcagcccttt taccccggca
    47101 gtgtagttca tcattgcggg aatgcggcga cgcgcgatgg ccccaaaaat cactgccgca
    47161 ctgatgcctg cattgggcag cattaagccg atgcgttcgc cttcaacact gtatttttca
    47221 aggatgcgtc caacaaacag cgttttcgta agcaatttgc gataggagtc tggggtaaag
    47281 ttgacgtctt cgacacattt cttcccggct ccgaagcggt acattgcact cagtaaagat
    47341 tcgtacagag tttcacgcgg gcgcaccgcc atacgcgctt ccatcattat ttgatgcagc
    47401 atttcgccag cgattttgcg acggtcacgg gcacgcggcg catccggcat cgccacctgc
    47461 gttggaggca aaatatgcag agtaatttgc gggaacaagc gacgtttaac cagacctttc
    47521 aggcggctga agtgcgtaag ttccgcccct tcaatacgca caggaataac cgttgcacca
    47581 gacttcgccg cgacaaaacc cgcgccatcg tagattttca tcagcgagcc tgtagtggtg
    47641 atgcgtcctt cagggaaaat caccactggt cggccctgtt ccaccagacg taccagatgt
    47701 ttaatagcca taggttgcgt cgggtcgaga ggaacaaagt cgataaatga tttcagccaa
    47761 cgcatatacc actgttggct tattgaggtg taaacggcaa acactggacg cacaggtaaa
    47821 aacagtccaa gcaaaatgcc atcaataaaa gagacgtgat taggcgtaat tagtacgcgc
    47881 tcgcccttca gtgcctgggt gtcacccgta acgcgaacgc gatacaaaac acggcacaaa
    47941 tttcgaaaaa agctaaaaag cattccaact ccctttgctc tgattcagta aaagcgaatg
    48001 gagggagatt acacgagata aagaacgcga gcgacagtaa attaggtgcg aaaaaaaacc
    48061 tgcgcatccg cgcaggttgg tgcaagagac agggtacgaa gagcgtaccg aataatctca
    48121 ccaatcaata cctctgggat cttgattgtg gtctgcacga cgactcttcg ccagcgagaa
    48181 aacgcaaagg aatgaaggga aatgcaacga ggtgtgtaaa ttgtcggtta ctgttacaga
    48241 ttgatgaccg gcaaaaaaaa acctgcgcat ctgcgcaggc tggtgtaatt catgtgctca
    48301 acacgaagtt gacttcacct atcaatacct ctgggatcac cactttagca acctgaagcc
    48361 aaacgccacc agcggtcaat cgcaacagcg tttcgcaaag tgtaaccaaa ggtttgcaat
    48421 ctcttttttt gtcttactta tttgcgtttg gctcacattc ccacgatgaa aacacgccac
    48481 cccttgaacc aacgggcgtt ttccgtaaca ctgaaacaat gtaagcgttt acccactaag
    48541 gtattttcat ggcgaccata aaggatgtag cccgactggc aggcgtttca gtcgccaccg
    48601 tttcccgcgt cattaataat tcacccaaag ccagcgaagc ttcccggctg gctgtgcata
    48661 gtgcaatgga gtctcttagc tatcacccga acgccaacgc ccgtgcgctg gcgcagcaga
    48721 ccactgaaac ggtcggtctg atcgttggtg atgtttccga tccgtttttc ggtgcaatgg
    48781 tgaaagcggt cgaacaggtg gcttatcaca ccggtaattt cttattgatt ggcaacggtt
    48841 accacaacga acaaaaagag cgtcaggcta ttgagcaact gatccgccat cgctgtgctg
    48901 cgttggtcgt ccatgccaaa atgatcccag atgccgattt agcctcatta atgaaacaaa
    48961 tgcccggtat ggtgctgatc aaccgtatcc tgcctggctt tgaaaaccgt tgtattgctc
    49021 ttgacgatcg ttacggtgcc tggctggcaa cgcgtcattt aattcagcaa ggtcataccc
    49081 gcattggtta tctgtgctct aaccactcta tttctgacgc cgaagatcgt ctgcaagggt
    49141 attacgatgc ccttgctgaa agtggtattc cggccaatga tcggctggtg acatttggcg
    49201 aaccagacga aagcggcggc gaacaggcaa tgaccgagct tttgggacga ggaagaaatt
    49261 tcactgcggt agcctgttat aacgattcaa tggcggcggg cgcgatgggc gttctcaatg
    49321 ataatggtat tgatgtaccg ggtgagattt cgttaattgg ctttgatgat gtgctggtgt
    49381 cacgctatgt gcgtccgcgc ctgaccaccg tgcgttaccc aatcgtaacg atggcgaccc
    49441 aggctgccga actggctttg gcactggcgg ataatcgccc tctcccggaa attactaatg
    49501 tatttagtcc gacgctggta cgtcgtcatt cagtgtcaac tccgtcgctg gaggcaagtc
    49561 atcatgcaac cagcgactaa ccgcagttaa agcaattcca gcgccagtaa ttcttcgatg
    49621 gtctggcgac ggcgaatcaa ccgcgcctga ccattatcaa acagaacttc tggtaacagc
    49681 ggacggctat tgtagttgga tgacattgat gcgccatatg cccctgtatc atgcagtacc
    49741 agataatcac ctgccttcac ttccggcaag gcgcgggttt caacatttcc cccttcctgc
    49801 tgggtaaaga catcgcccga ttcacataac ggcccggcga cgacggtttc caccgttggc
    49861 gcgtgttcca gagaacgacc atcagctgcc agggcactga tatggtggta actaccgtac
    49921 attgccgggc gcatcagatc gttgaacccg gcatcaacca gcacaaagtg gcggctaccc
    49981 atttgtttga cgctccgcac ctgagtaatt aatacgccag actgcgctac caggaagcga
    50041 cccggttcaa tttccagttt cacagggtgg cccaaatggc gagcgatttg ctcacgcgcg
    50101 gcattccaca gaccataata atgttcggta tcaaccgcct cttcaccctg ttgataagga
    50161 atagaaagcc caccgcccgc agaaatagcc tgtaaatcct gaccgaattc gataacctga
    50221 cgcaccatag caccacacac ctgctccaga tgggcataat caacgccaga accaatgtgc
    50281 atgtgaatgc cgactagctg cagatgatga cgttgtatca cgtccagtgc ggcgggcaga
    50341 tcggtgtacc agataccgtg cttgctgttt tcgccaccgg tattggtttt ttggctatgt
    50401 ccgtgaccaa accccggatt aacgcgcagc catacccgat gccctggcga aacctggccc
    50461 agttggtcga gcgtatcaac agaacccgca ttcaccggaa tttgcaattc actgacgcgt
    50521 tcaagcgtcg cctgatcgat aacatctgcc gtaaaaacaa tatcatcggg gtgcgtttgc
    50581 ggattgtaac ccgccgccaa cgcacgctct atttcgccta acgagacgga atccactttc
    50641 acgccctgct cacgcattaa gcgcaaaata tgaatattgg aacaggcttt ctgtgcaaag
    50701 cgcaccactt caaactgttt cagcgctgca atctgccgac gaataatttg cgcatcgtag
    50761 acccacaccg ggcagccaaa ttcagcgggc aaacgcagca gattttcggc ggtgagatcg
    50821 gtatcggtgc tgaacagtga atgtggcata acaaactcca gatgagtgct tttttatgat
    50881 tacgccacat cataaaaaga ataaaaaata tcgatttatg tcgagtctat gcaaaaatga
    50941 tatggattac cggattgcga gagagcgcta atggccgccg ttaacttacg tcatattgaa
    51001 atttttcatg cggtaatgac cgccggaagc ctgactgagg cagcacacct gctacacacc
    51061 tcacagccaa ccgtcagccg cgaactggcg cgctttgaga aggtgatcgg gctgaaattg
    51121 tttgagcgcg tacgtggacg attacatcct accgtgcaag gactgcgtct gtttgaagaa
    51181 gtgcaacgat cctggtacgg actggatcgc attgtcagcg ccgcagaaag tctgcgcgag
    51241 tttcgccagg gagaactgtc tattgcctgc ctgccggtct tttcgcaatc ttttttaccg
    51301 cagctcctgc aaccctttct ggcacgttat cccgatgtca gcttaaatat cgtgccccag
    51361 gaatcaccgc tacttgaaga gtggctctcg gcccagcgtc atgatttagg actcactgag
    51421 acactccata cgcctacggg aacagaacgt accgaattac tctctttaga tgaagtgtgt
    51481 gtgttacctc cgggtcatcc gctggcggta aaaaaggtat taacgccgga tgattttcag
    51541 ggtgagaatt acatcagcct ttcccgtact gacagctatc gccagttgct ggatcagcta
    51601 tttactgaac atcaggttaa acgacgcatg atcgtagaaa cccacagcgc cgcgtcagtc
    51661 tgcgcaatgg tacgggcggg ggtaggtatt tcggtggtta acccgctcac cgcactagat
    51721 tatgcggcaa gcggtttagt ggtgcggcgg ttcagtattg cggttccgtt caccgtcagc
    51781 ctgatccgcc ccctgcaccg cccgtcatca gcgctggttc aggcgtttag tgggcattta
    51841 caagcggggt taccgaaact ggtcacttct cttgacgcta ttttgtcgtc agctacgaca
    51901 gcataaaagc gacagcatcc tcggcatgga tcgccgcggt atcaaacaca ggcaaaacac
    51961 tgcgctcttc tggcaccagt aaaccaattt ctgtgcagcc aaaaatgacg ccctgtgcgc
    52021 cctgttctgc aaggcgagca atcacttgcg cataataagc gcgtgacgct tcggtaaatt
    52081 gccccagaca cagttcttca aaaataatct gattaatttt cgcccgttca tccgcttcag
    52141 gaataagaca attgatggaa aattgttccg tcagccgccc gcgataaaaa tcctgttcca
    52201 tggtgtaacg cgtacccagc agcgccacac gagtcattcc ggccccggta attgcacgtc
    52261 cggtggcatc cgcaatgtgt aagaaaggca gagtgcaacg tgactcaatg gcatccgcca
    52321 ctttatgcat cgtattggta cataacacaa taccttctgc gcccgcccgc tgtaagccaa
    52381 gcgccgcctc agccagaatg tccccggttt tatcccattc cccgcgacgc tggcactctt
    52441 ctatttcatg aaaatcgacg ctatgtagca gcatttgcgc agagtgaagc ccaccaagcc
    52501 gctgtttaat gccttcattt atcagacgat agtaaggaat ggtggattcc cagctcattc
    52561 ctcccagcaa accaattgtt ttcattcctc tctcctgatg tgtgttaccg caacaaggct
    52621 gaataaggaa cgagacaaac gcctcaacgg ccaggtgccc aatctctatt aacgaaaaaa
    52681 gggccggatg tacagcacat ccggcccgtg aaatcagacg ccgatatttc tcaacttctc
    52741 gcctgccatc agtttgcgtt cgatatgttc cagcgtgaca tttttggttt ccggaatgag
    52801 ccagaaagta atacccacaa acgcaatgtt cagcgcagtg tagagccaga acgtaccggc
    52861 agcgccaatg ctatcaagca gtgtcaggaa ggccgcgccg ataatcatat tcgacaccca
    52921 gtttgtcgtc gtcgaacagg taataccgaa atcgcggcat ttcagcggct gaatttcaga
    52981 gcacaggatc cacaccactg gcgcggcgct catcgcataa ccggcaatac acatcatcgt
    53041 catgccaaca gagagccagg acaagccact ggaagccgta ccgttatcaa actgcatcag
    53101 gcaatagccc agcaccagag tgcctaacgc catcacgctg aaaccaattt tcagagccgg
    53161 tttacgacct gctttatcta ccgtaaagac cgcaatgaag gtcgcgaaca taaaggtcag
    53221 tcccacgacc agagtcgcaa tcatctgttg ttctgtggtc gtaaagcccg ccattttgaa
    53281 gatacgcggc gcgtagtaca tgatgatgtt cataccggta aactgctgca tcgcctgcaa
    53341 caacataccg agaaacacag cacgacggac gttacggttg atcttaaaca gtgcccaacc
    53401 gccctgtttt aacttcaggc tttcacgaat ttcgttgagt tcttctcgcg ctttttccga
    53461 cgtatcgcgc agcatacgca acacttcttc cgcctcaata tgacgcccct tttccgccag
    53521 ccagcgcggg ctatttggca ggaagaccac cagaataatc agcagaactg ctggtaaagc
    53581 aagaaccccc aacattgcgc gccagttacc gctataactg aacgctgtat cggataaaaa
    53641 cgccagcacg atgccgagtg tgaccatcaa ctggtacata ctgatcatct taccgcgaac
    53701 gttttcactt gccatttcag aaagatacag aggagcggtg taagacgcga tcccgacagc
    53761 aatgcccagc accacacgag cggcgattaa catctctacg ctggtcgcaa aagcggaccc
    53821 tatagaaccg agtacaaaca ggatggcccc cgccatcagg ctgtatttac gccccaggcg
    53881 gaacgacagc caaccattaa acagcgcacc aattgctgcg ccgagcatca tgctactaac
    53941 cacccattcc tgcaaacgac tggtcagcac aaagtgatcg gtaatgaacg gcaacgctcc
    54001 ggcgattacg ccgatatcaa gaccaaataa caatcctgcg accgcagcag ctaccgaaac
    54061 aaacatattc atacgccgcg tatcacgcaa agaacgtggc gttaaagcag attccgtatt
    54121 gatagtaacc attttttcct gccagcagag agtaagacat tgtgaaaaaa tacgtgaaca
    54181 attcacacag gtgtcaggtc ggaaacagca taaatatgga ttaaattgct gcgacatgcc
    54241 gttatgtgat ggatattcca attttaaaat taagttgaat tattgagatt attattaacc
    54301 tcctaatttt aaagcagata aaattcataa agttcattaa ttgataattg atatggatta
    54361 tttcataacc atgatatgga ttatgatgat ctagagatat aaaaaaccct gccatgcggc
    54421 agggtcataa aagtaagagg aatgaattaa cgcgccagcc aaccgccatc cacagcaatg
    54481 gtataaccat ttacataatc tgaagcgctg gaggcaagaa acactaccgg ccccatcagg
    54541 tcactcggca gtccccaacg gccagctgga atgcgatcga gaatttccgc gctacgttgt
    54601 tcatctgccc gcagttgttg ggtattgttg gtcgccatgt aacccggagc tatcgcatta
    54661 acattaatgt tgtgtttagc ccattcgttt gccatcaatc gcgtcacacc catcacgccg
    54721 ctttttgatg cggtataaga aggcacacgg atcccgccct ggaaggagag cattgacgcg
    54781 atattgataa tcttgccgcc attgccttgc gcgataaagt gtttcgccgc tgcctgagac
    54841 atgaagaata cgctcttgat attcaggttc atgacatcgt cccagtcttt ttcgctgaac
    54901 tcgagagcgt cttcgcggcg aatcaatccg gcgttattca ccaggatatc aatatgacca
    54961 aattcagcta ccgcgcgatc cagcagtcca ggaatgccat caatctttcg cagatcggcg
    55021 gtcaggctta aaaaacgacg ccccagcgcc gtgacctgct cgatggtttc agtcggttca
    55081 acgatgttaa tgccaacaat gtcacagccc gcttgcgcca gccccaacgc catcccctgg
    55141 cccagcccag tatcacaacc agtgacgacc gcaactttac cttggagaga aaatgcactt
    55201 aaaatcataa caacacctta ttcgtttatg cccacaacta gcgcaaatct ttaacgccca
    55261 catggtccat atcattaaag acctggtttt caccgaccat gccccagata aaggtataag
    55321 ctttggttcc gacgccggaa tggatcgacc agctcgggga gatcaccgcc tgctcgttat
    55381 gcatcacaat gtgacgcgtt tcttgcggct gccccatcat gtggaaaacg caggcgtcat
    55441 catccatatt gaaatagaaa taaacttcca tccggcgctc gtgggtgtga cacggcatgg
    55501 tgttccacaa gttacctgga gccagctccg tcagccccat actcaattga caggtttcca
    55561 gtacatccgg gacaaaatat ttgttaatcg tgcgacggtt actggtgagg ttatcgccta
    55621 acgtgactgg agatacttcg tccggtgtga cttttttggt gggatacgtc gtatgcgcgg
    55681 gtgcgcaatt gtaataaaac ttcgccggag tggcggtatc aatactggca aagacaactt
    55741 cttttgcacc tttaccaaca tacagggcgt cgcggtgacc gatttcatag cattgaccat
    55801 cgacagtaat cgtaccggca ccgccaatat tgataacacc taactcgcga cgttcaagga
    55861 aatagcttac gccgagttgt ttaccaactt ccccgccaac ggaaaccgtt ttagttatcg
    55921 gcataatgcc gccaacaata attcggtcaa tgtggctgta aaccatggtg tactcatcgg
    55981 cgacaaatac cttttcaacc aaaaattcat tgcgcagccc ttgggtatcc agcgtttttg
    56041 cgtgcgcacc gtggatgctc tgtcttacgt ccacatcaac ctccgataat cagtgaagcg
    56101 tgaaaataga aaacgatagt gaaacactgt ttcgttttct ctataaaggg atgatatgcc
    56161 catgggctag ggttttcaat taaaaataaa acaatgtttt atttttaatt taaggattaa
    56221 aaaaagtgca gtgtcgatca cgaatatgtc atgagaatgg aaaaatgagg gagcccagaa
    56281 aatgtatgtc gacttgccag ccatcattat tcaacagatt aatgacggca gatatttatc
    56341 ggtaaggagg aaatgcagag ctactcgtca cgttcaatgg tcaatgccac gccctggccc
    56401 ccgccgatac aaagcgttgc cagccctttg cgggcattac gtttcaccat ttcatgaacc
    56461 agagaaacca ggattcggca accggaagcg cctatcgggt gaccgagtgc gatcgcgcca
    56521 ccattgacat tgacccgacg ctcatcccac tcaagcatct tgccaaccga aagcgcctgt
    56581 gcagcaaacg cttcattagc ctcgataaga tcgacatcag ccaactgcca gcctacacgc
    56641 tccaggcaac ggcgggtcgc atacaccggc gcaattccca tcaatgccgg atctacacca
    56701 acgctggcaa atgcgcggat gcgggccagc acgggtaaat tcaacgctcg tgctttggct
    56761 tcgctcatca tcattaccgc agctgcgcca tcgtttatgg atgatgcatt ccccgcggtc
    56821 acagaaccga gactatcaaa tgaaggattt aaacgggcta aaccttctgc actggcgtca
    56881 gtgcgtggct gttcatcggt atcaacaacc aacgtctgac cgttactttg gatcattacc
    56941 gggacgatct catctttaaa tcgtccggcg tcaatcgccg ctcgcgcttt ttgttgcgag
    57001 ctaagtgcgt aagcatcctg caactgacgg ctgatgccat attcgcgagc cagattttcg
    57061 gcggtgacac caatatgata atcattgaag gcatcccaca acccatcatg caccagactg
    57121 tcaaccaact ggctattgcc aagctgttca ccggtgcggc tatcagtcag aacatgtggt
    57181 gcgcggctca tgttttcctg gccacctgcg atgacaatat cagcctcgcc acactgtatc
    57241 gcctgagtag ccagatgcag tgctttaaga ccggaaccgc aaacgtcatt aatcgtgatt
    57301 gcagaaacgc tgttaggaag accaccttta atagccgatt gccttgccgg attctgccct
    57361 gcccctgcag tcaacacctg accaagaatt acttcatcca ccgcatatgc aggaacgccg
    57421 gtacgttcta ttaacgcttt cacgaccaga ctaccaagtt ccacggcgga atgacccgct
    57481 aacgcaccac gaaagcagcc gataggtgtc cgtaacgccc cgacaatcac aacgtctttc
    57541 atcacaacct cgcaccaatt aacagtgcaa tagtaaatga ttgttaatta cagttatctt
    57601 aattgtttaa aaaaagtgat ttttatcaca aaggaaatat gcctgagcag tagtcagagt
    57661 cataactggc acgtaaggtt tgcaaccact aacccaccaa tagatgggta gatagggcgt
    57721 taatctccca tacttaactt ggtttatggt aaattgccct ccattttgtt taatttgtag
    57781 atgatacgtt cagataatgt ctaatatttg gtctaaagaa gaaactctgt gaagtttcgc
    57841 gctctatggc acagccgttg atgcaggcac gctattcctt cctattcagt taggttcggc
    57901 aggggctgtg gtcctgttta ttactgctct ggtcgcctgg cctttaacat attggccaca
    57961 taaagcctta tgccagttca tcctctcatc aaaaacatca gcaggtgaag ggataacggg
    58021 cgcggtaaca cactactatg gcaagaagat tggtaatctg attaccacgc tgtacttcat
    58081 cgcctttttt gtcgtcgtgt tgatatatgc agtggcaatt accaactcac ttacggaaca
    58141 gctggcaaag catatggtta ttgatcttcg catccgtatg ttggtgagtc tgggtgttgt
    58201 attaattctg aatctcattt ttctgatggg acgtcatgcc actattcggg taatgggatt
    58261 tttggtattc ccattgattg cctatttctt atttctttcc atttaccttg tcggtagttg
    58321 gcaacctgat ctattaacaa cccaggtaga gttcaatcag aatacccttc accagatatg
    58381 gatatcgatt cccgtgatgg ttttcgcctt tagccatacg cccattattt ctacgtttgc
    58441 catagacaga cgtgaaaaat atggcgaaca cgctatggat aaatgcaaaa aaattatgaa
    58501 agtcgcttat ctcatcatct gcataagtgt actgttcttt gtctttagct gcctgctttc
    58561 tattccatct tcgtatattg aagcggctaa agaagaaggg gtcaccattt tatcggcgct
    58621 ttctatgctg ccgaacgccc cagcatggtt gtcaatttcc gggattattg tcgcagtagt
    58681 tgcgatgtcg aaatcattcc tgggtacgta ctttggcgtt attgaaggtg ccacagaggt
    58741 cgtcaaaaca acactacagc aggttggtgt aaagaaaagt cgtgcattta accacgcact
    58801 atcaattatg ttggtatcgc tgattacctt cattgtttgt tgcattaacc cgaacgcgat
    58861 ttcgatgatt tacgcgatca gcggcccgct cattgccatg atacttttca tcatgcctac
    58921 gctgtcaacg tatctcatcc cggcgcttaa accctggcgt tccatcggaa atctgattac
    58981 gctgatcgtg ggtatcctgt gcgtatcggt aatgttcttt agctaatatc cgttgtggat
    59041 ggttcaaaat tgctacaatt ttgaaccatt ccatttttca taatattcat ttgttattat
    59101 tccgccctat cacttctctc gtcatatcct agaattatca gttagttaaa cacacaaaga
    59161 gatctgaaca tcttaaataa gactatttaa gatgcataac ttagattcgc aagatatctt
    59221 ctggtcattc aaaaacaatt tgcgatatag atattctcat accatgagat agttatctgg
    59281 accacgttcg accatgcaag attaaatcag tgaaacataa tcatatttaa taacgagaaa
    59341 tgcattttta aatatcttaa tcagaattat agcaaataca gattaatcgc aatttttccc
    59401 agcaaaatta caaaatcaag gtgtattgac atcatcaatc accaatgaaa ttatgcaatt
    59461 atatacggat agggaggttc ttaacatggg gctttgtagt cgttataaaa gtcttacatg
    59521 caatagttgc tcaatgcatt gccaaataat gccagaagag tcaccgcgtt tgcagtactg
    59581 cgcaaactcg t
//
LOCUS       DAJTUI010000014        58573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTUI010000014 DAJTUI010000000
VERSION     DAJTUI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.014"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..1100)
                     /locus_tag="OO464_000931"
     CDS             complement(<1..1100)
                     /locus_tag="OO464_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR6226172.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="OO464_000932"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="OO464_000932"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6226173.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="OO464_000933"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="OO464_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6226174.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="OO464_000934"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="OO464_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6226175.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="OO464_000935"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="OO464_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6226176.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="OO464_000936"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="OO464_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6226177.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="OO464_000937"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="OO464_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6226178.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="OO464_000938"
     CDS             complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="OO464_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR6226179.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="OO464_000939"
     CDS             complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="OO464_000939"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR6226180.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="OO464_000940"
     CDS             complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="OO464_000940"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR6226181.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="OO464_000941"
     CDS             complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="OO464_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6226182.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="OO464_000942"
     CDS             complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="OO464_000942"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR6226183.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10283..11701
                     /gene="puuA"
                     /locus_tag="OO464_000943"
     CDS             10283..11701
                     /gene="puuA"
                     /locus_tag="OO464_000943"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6226184.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11850..12014
                     /locus_tag="OO464_000944"
     CDS             11850..12014
                     /locus_tag="OO464_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226185.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12004..13389
                     /gene="puuP"
                     /locus_tag="OO464_000945"
     CDS             12004..13389
                     /gene="puuP"
                     /locus_tag="OO464_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6226186.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13523..13768
                     /locus_tag="OO464_000946"
     CDS             13523..13768
                     /locus_tag="OO464_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6226187.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14081..15724
                     /gene="sapA"
                     /locus_tag="OO464_000947"
     CDS             14081..15724
                     /gene="sapA"
                     /locus_tag="OO464_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6226188.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15721..16686
                     /gene="sapB"
                     /locus_tag="OO464_000948"
     CDS             15721..16686
                     /gene="sapB"
                     /locus_tag="OO464_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6226189.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16673..17563
                     /gene="sapC"
                     /locus_tag="OO464_000949"
     CDS             16673..17563
                     /gene="sapC"
                     /locus_tag="OO464_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6226190.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17563..18555
                     /gene="sapD"
                     /locus_tag="OO464_000950"
     CDS             17563..18555
                     /gene="sapD"
                     /locus_tag="OO464_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6226191.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18557..19363
                     /gene="sapF"
                     /locus_tag="OO464_000951"
     CDS             18557..19363
                     /gene="sapF"
                     /locus_tag="OO464_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6226192.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19431..19784
                     /locus_tag="OO464_000952"
     CDS             19431..19784
                     /locus_tag="OO464_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6226193.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20152..20940
                     /gene="fabI"
                     /locus_tag="OO464_000953"
     CDS             20152..20940
                     /gene="fabI"
                     /locus_tag="OO464_000953"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6226194.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21084..22211
                     /locus_tag="OO464_000954"
     CDS             21084..22211
                     /locus_tag="OO464_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR6226195.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22279..24213
                     /gene="rnb"
                     /locus_tag="OO464_000955"
     CDS             22279..24213
                     /gene="rnb"
                     /locus_tag="OO464_000955"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6226196.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24291..24394)
                     /locus_tag="OO464_000956"
                     /pseudo
     CDS             complement(24291..24394)
                     /locus_tag="OO464_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24448..26422
                     /gene="pdeR"
                     /locus_tag="OO464_000957"
                     /pseudo
     CDS             24448..26422
                     /gene="pdeR"
                     /locus_tag="OO464_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26570..26743
                     /locus_tag="OO464_000958"
     CDS             26570..26743
                     /locus_tag="OO464_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226197.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26833..27582
                     /locus_tag="OO464_000959"
     CDS             26833..27582
                     /locus_tag="OO464_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6226198.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27851..28069
                     /gene="osmB"
                     /locus_tag="OO464_000960"
     CDS             27851..28069
                     /gene="osmB"
                     /locus_tag="OO464_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR6226199.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28195..28521)
                     /gene="yciH"
                     /locus_tag="OO464_000961"
     CDS             complement(28195..28521)
                     /gene="yciH"
                     /locus_tag="OO464_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6226200.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28521..29258)
                     /gene="pyrF"
                     /locus_tag="OO464_000962"
     CDS             complement(28521..29258)
                     /gene="pyrF"
                     /locus_tag="OO464_000962"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6226201.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29451..30620)
                     /gene="lapB"
                     /locus_tag="OO464_000963"
     CDS             complement(29451..30620)
                     /gene="lapB"
                     /locus_tag="OO464_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6226202.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30627..30935)
                     /gene="lapA"
                     /locus_tag="OO464_000964"
     CDS             complement(30627..30935)
                     /gene="lapA"
                     /locus_tag="OO464_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6226203.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31084..31848)
                     /gene="pgpB"
                     /locus_tag="OO464_000965"
     CDS             complement(31084..31848)
                     /gene="pgpB"
                     /locus_tag="OO464_000965"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6226204.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32018..32608
                     /gene="ribA"
                     /locus_tag="OO464_000966"
     CDS             32018..32608
                     /gene="ribA"
                     /locus_tag="OO464_000966"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6226205.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32672..35347)
                     /gene="acnA"
                     /locus_tag="OO464_000967"
     CDS             complement(32672..35347)
                     /gene="acnA"
                     /locus_tag="OO464_000967"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR6226206.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35511..35606)
                     /locus_tag="OO464_000968"
     CDS             complement(35511..35606)
                     /locus_tag="OO464_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226207.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35720..35887)
                     /locus_tag="OO464_000969"
     CDS             complement(35720..35887)
                     /locus_tag="OO464_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226208.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35890..36054)
                     /locus_tag="OO464_000970"
     CDS             complement(35890..36054)
                     /locus_tag="OO464_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6226209.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36349..37323)
                     /gene="cysB"
                     /locus_tag="OO464_000971"
     CDS             complement(36349..37323)
                     /gene="cysB"
                     /locus_tag="OO464_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6226210.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37533..40130)
                     /gene="topA"
                     /locus_tag="OO464_000972"
     CDS             complement(37533..40130)
                     /gene="topA"
                     /locus_tag="OO464_000972"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6226211.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40510..40761
                     /locus_tag="OO464_000973"
     CDS             40510..40761
                     /locus_tag="OO464_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6226212.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40797..41846)
                     /gene="sohB"
                     /locus_tag="OO464_000974"
     CDS             complement(40797..41846)
                     /gene="sohB"
                     /locus_tag="OO464_000974"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6226213.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42066..42824
                     /locus_tag="OO464_000975"
     CDS             42066..42824
                     /locus_tag="OO464_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6226214.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42821..43411
                     /gene="cobO"
                     /locus_tag="OO464_000976"
     CDS             42821..43411
                     /gene="cobO"
                     /locus_tag="OO464_000976"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR6226215.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43451..44323)
                     /gene="rluB"
                     /locus_tag="OO464_000977"
     CDS             complement(43451..44323)
                     /gene="rluB"
                     /locus_tag="OO464_000977"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6226216.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44424..45044)
                     /locus_tag="OO464_000978"
     CDS             complement(44424..45044)
                     /locus_tag="OO464_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6226217.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45041..45922)
                     /gene="yciV"
                     /locus_tag="OO464_000979"
     CDS             complement(45041..45922)
                     /gene="yciV"
                     /locus_tag="OO464_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6226218.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46060..46104
                     /gene="trpL"
                     /locus_tag="OO464_000980"
     CDS             46060..46104
                     /gene="trpL"
                     /locus_tag="OO464_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR6226219.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46081..46174
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46196..47758
                     /gene="trpE"
                     /locus_tag="OO464_000981"
     CDS             46196..47758
                     /gene="trpE"
                     /locus_tag="OO464_000981"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6226220.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47758..49353
                     /gene="trpD"
                     /locus_tag="OO464_000982"
     CDS             47758..49353
                     /gene="trpD"
                     /locus_tag="OO464_000982"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6226221.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49354..50715
                     /gene="trpCF"
                     /locus_tag="OO464_000983"
     CDS             49354..50715
                     /gene="trpCF"
                     /locus_tag="OO464_000983"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6226222.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50727..51920
                     /gene="trpB"
                     /locus_tag="OO464_000984"
     CDS             50727..51920
                     /gene="trpB"
                     /locus_tag="OO464_000984"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6226223.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51920..52726
                     /gene="trpA"
                     /locus_tag="OO464_000985"
     CDS             51920..52726
                     /gene="trpA"
                     /locus_tag="OO464_000985"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6226224.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53106..53285
                     /locus_tag="OO464_000986"
     CDS             53106..53285
                     /locus_tag="OO464_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR6226225.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53371..53871
                     /locus_tag="OO464_000987"
     CDS             53371..53871
                     /locus_tag="OO464_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6226226.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53917..54423
                     /locus_tag="OO464_000988"
     CDS             53917..54423
                     /locus_tag="OO464_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6226227.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54483..55121)
                     /gene="ompW"
                     /locus_tag="OO464_000989"
     CDS             complement(54483..55121)
                     /gene="ompW"
                     /locus_tag="OO464_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6226228.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55361..55450
                     /locus_tag="OO464_000990"
                     /pseudo
     CDS             55361..55450
                     /locus_tag="OO464_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55478..56221
                     /locus_tag="OO464_000991"
     CDS             55478..56221
                     /locus_tag="OO464_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6226229.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56251..56790
                     /locus_tag="OO464_000992"
     CDS             56251..56790
                     /locus_tag="OO464_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6226230.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56895..57293
                     /gene="yciA"
                     /locus_tag="OO464_000993"
     CDS             56895..57293
                     /gene="yciA"
                     /locus_tag="OO464_000993"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6226231.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57333..58061)
                     /gene="tonB"
                     /locus_tag="OO464_000994"
     CDS             complement(57333..58061)
                     /gene="tonB"
                     /locus_tag="OO464_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR6226232.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58276..58572
                     /locus_tag="OO464_000995"
     CDS             58276..58572
                     /locus_tag="OO464_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR6226233.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        14061 a        15144 c        14644 g        14724 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaataatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggaagggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccatgtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg agctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaaaaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataacttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
     4741 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
     4801 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
     4861 cagcgggttc ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt
     4921 cgcgttaccg accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat
     4981 taagtccggc ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc
     5041 gctttgcact tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc
     5101 ggcaaccagc tcttttggcg caacgttgaa accgcactcg ccctgcaccg gttcgaaaat
     5161 aatggccgcg acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag
     5221 ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc
     5281 agggaacggg ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat
     5341 atatgtacga ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc
     5401 gcgagcaatt ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt
     5461 agcctgcccg ctcactgggg caagggtgtt aattttctcc gccagcgtga cgtagctttc
     5521 gtacggcaca atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc
     5581 gaccagatca gggtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat
     5641 gtattcgttg ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt
     5701 acacatcacg ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt
     5761 gctcatgttt cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat
     5821 cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc
     5881 cggggaacgg gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca
     5941 gcagttcagc aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac
     6001 cctgcatgta atagatgttg gtatcaaggc ggccgaactg cggcatgtgc gacaaggtca
     6061 gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt
     6121 ttagtagttt cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta
     6181 cacccccgcc gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt
     6241 tacagtcttc cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc
     6301 gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta
     6361 ctttatcgcc cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat
     6421 tggcagtccg aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat
     6481 acacgcgccc gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt
     6541 gaatatgccc accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc
     6601 ggcgaatagc gttgacgtcc agcagctcca gctgtttatt gccgtagcgt acccagttct
     6661 ctttctgctc ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg
     6721 ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct
     6781 cgaacatcat gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt
     6841 cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc
     6901 cgatgcgtga ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg
     6961 aggagagccc ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca
     7021 gtgtgtcgaa tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg
     7081 tcatttcagg cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca
     7141 tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata
     7201 tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg
     7261 tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg
     7321 gcaagctgca gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa
     7381 atctcttcgc ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg
     7441 gccgctgcca gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca
     7501 cgaataaagg tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt
     7561 gcgggatcaa gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag
     7621 aattcatcgg cgatgctctc ttccagtaac aaacgcgttc cggcgatgcg cacctggccc
     7681 tggttgtaga agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg
     7741 gcgaaaacga tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta
     7801 tcacccgcgt ctttcagcag ttgtttaccc gtacgcgtgg agccggtaaa ggcgatggca
     7861 tcgatatcgt tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg
     7921 ttcaacacac catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg
     7981 agcggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg
     8041 agtttccagc aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg
     8101 accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac
     8161 actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca
     8221 cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc cagctcttcg
     8281 gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt agccggagaa
     8341 gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc acggtcgata
     8401 tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg atcaacggtt
     8461 tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa gcggttttca
     8521 atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt cattatgact
     8581 cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag agatccccgg
     8641 gctgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc attgcgctta
     8701 cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc
     8761 aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt
     8821 gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca ccttccagta
     8881 ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt
     8941 acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg
     9001 acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat
     9061 caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc ttcagcagct
     9121 tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt
     9181 gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc tggcggattt
     9241 ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg
     9301 tagtggtcag agtcgctgtt tttcagcgat atggtgctgc caagcggtga taaagccctc
     9361 gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc actgtacgcc
     9421 cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc
     9481 ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag agtttaccca
     9541 aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa cctgaacttc
     9601 atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat gttccagcaa
     9661 ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca ccagttcttg
     9721 taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat taattatcgc
     9781 catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt caccatatag
     9841 gtgcggctgc acattgctgg gactactagg aagataaatg ccatcgagtt tcggcaaaag
     9901 ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca agccgcctgc
     9961 gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac ccttaagcct
    10021 gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca tgctcaatct
    10081 cacaaaatgg actaaattat cgccattact gcgctttaaa tgacctttat gttcaatatt
    10141 ttttcaatct agcagtggat tttcattttt gcaaactcaa tttaacattt gacaaacatt
    10201 tggtttgcat acagattcga atgatggtca ttatatttta cgctttgata acgagcggaa
    10261 aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga actttgttca
    10321 gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct acctggagcg
    10381 ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt gcttccgtgg
    10441 aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt tcccggcctc
    10501 ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg gtcaggaaat
    10561 gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt ctgccgccga
    10621 tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg ctccctttga
    10681 cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc gcggattgtt
    10741 cccggttgta gcggtagagc tggagttcta tttactggat cgccagcgcg acgctgaagg
    10801 ggatctgcaa ccgccctgcg cgcctggcac cgatgaccgc aatacacaaa gccaggttta
    10861 ctccgttgat aacctcaacc acttcgccga cgtgctcaat gatattgatg aactggcgca
    10921 gttgcagctg attccggcag atggcgcggt cgctgaggcc tcgccgggtc agtttgaaat
    10981 caacctttac catactgata acgtgctgga agcctgcgat gatgcgctgg cactaaaacg
    11041 cctagtgcgt ctgatggcag aaaagcataa gatgcacgcc acttttatgg cgaagccgta
    11101 tgaagagcac gcgggcagcg ggatgcatat ccatatcagt atgcaaaata atcgtggcga
    11161 gaatgtgctt tctgacacag aaggcgaaga ttcgccgctg ctgaaaaaga tgctcgccgg
    11221 gatgattgac ctgatgccgt cgtcgatggc gttgctggca ccaaacgtga actcgtatcg
    11281 ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg tggggccata acaaccgcac
    11341 cgtcgccctg cgtattccgt gcggtgaccg tcataatcac cgcgtggaat atcacgtggc
    11401 gggtgccgat gccaacccat atctggtgat ggcagcgatt tttgccggta ttttgcatgg
    11461 ccttgataac gagctgccgt tgcaggaaga agtcgaaggc aacgggctgg aacaggaagg
    11521 cttacccttc ccgattcgcc agagcgatgc cctgggtgag tttatcgaga atgatcacct
    11581 gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac gcctgtaaaa atgatgagct
    11641 gttgcagttt gagcgcctca tcacagaaac agaaattgag tggatgttga aaaacgcgta
    11701 attccccttg cggggttgtt tcatcggcct gagcggagcc aggcctgaca tttgctggta
    11761 atccagacat ttcccggatg tcgcgttatg gagcgcaggc ggcaacgggc ttccgttaac
    11821 gattaagact gtgacgagga aatgagatga tgccgatgat taaatccccg catggcgagg
    11881 gaggctgcgt atgcgcccct cctgctactg actggactcc gccccctttg cttcccttgc
    11941 taaacaggtt tgatttcagg tcgacacgac cgcaaacctt attacgcagg ggaggcagca
    12001 attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac ccgtctgcga
    12061 aaatcactga aattgtggca ggtggtaatg atgggtctgg cctatctcac gccgatgacc
    12121 gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc ggcgtcctat
    12181 ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact ggttcgccag
    12241 tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaaccc gcacgtcgga
    12301 tttatggtcg gctggtcatc actgctggat tatctctttt tgccaatgat caacgttttg
    12361 ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt gtgggtggta
    12421 accttcgtcg ccattttaac cgccgcgaat ctgaagagcg tcaacctggt cgctaacttc
    12481 aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt cctggtggtt
    12541 cagggactgc ataaaggaga aggcgttggc accgtctggt cacttcagcc gtttatcagc
    12601 gagaacgcgc acctgatccc gattattacc ggtgcgacga ttgtctgttt ctcgttcctc
    12661 ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg cgtgatcccg
    12721 aaagccatct tcctgacggc ggtatacggc ggcgttatct ttatcgcggc gtcgttcttt
    12781 atgcagctgt tctttcccga tatcagccgc tttaaagacc cggacgccgc actgcctgaa
    12841 attgcgctgt acgtcggcgg caagctcttt cagtcgattt tcctctgcac cacgtttgtg
    12901 aacacattag cgtctggcct ggcttcacat gccagcgtgt cgcgtctgct gtatgtgatg
    12961 gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa atggcggact
    13021 ccggcactca acgtcattat ggtcgggatt gtcgcgctgt cggcgctgtt ctttgattta
    13081 gtcaccgcga cagcactgat taacttcggt gctctggtgg cgtttacctt cgtgaatctg
    13141 tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc
    13201 cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg ggttaaccta
    13261 gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc atatttgtgg
    13321 tatctgatcc gccgctatcg caaagtgccg ctgtacgaag gtgacagaac gccggtgagc
    13381 gaaacgtaat agccggatgc gcggcgcatt atcctgcctg agggtaacgc gccgcgccat
    13441 taactataaa aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc
    13501 gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc
    13561 cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagag gctgaacgca caccgctggc
    13621 ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc
    13681 ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg agatcgcaga
    13741 gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg tcagtatcgt
    13801 tgtccggata aagcgttcac gccgcatccg gctaacaatg acacgcactg cagcgtgctt
    13861 taatgggttt ctcacgatcg taccgctctg ctcctcgcta agttgttctg tgagttatag
    13921 ttatagagaa atcacgggta cgaatgttga acagatggcg acgccacctg ttcaacgacc
    13981 aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat
    14041 attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg tattatcgtc
    14101 tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc
    14161 gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt
    14221 taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga
    14281 tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg ccgaaagctg
    14341 ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg ttccgtttca
    14401 aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt
    14461 ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta
    14521 cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg ataatcatac
    14581 cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg caacccatta
    14641 tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca
    14701 actcgaccgt caaccggtcg gcaccggtcc gtatcagttg tcggaatacc gcgccgggca
    14761 atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt
    14821 agtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ccggggaatg
    14881 cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt
    14941 gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc
    15001 gccgctaaat aatcccgctg tccgccatgc actggcactg gcgattaata accagcgcct
    15061 gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc
    15121 gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca
    15181 gttgaaggcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca cacgttcgca
    15241 ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt
    15301 tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcgc ggttgatgga
    15361 tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt
    15421 cttccgtccg ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg
    15481 caatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcagcgcg
    15541 tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattaccta ttctgccgct
    15601 ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc
    15661 gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc
    15721 atgattatct tcaccttacg ccgcattttg ttattgattg tcaccttgtt cctgctgacc
    15781 ttcgttggct tcagcttgag ctatttcact ccacacgcgc cgttacaagg cgcgtcactg
    15841 tggaatgcct gggtgttctg gtttaatggc ctgatccact gggattttgg tgtttccagc
    15901 attaacggtc aaccaattgc cgaacagtta aaagagctct tcccggcgac gatagaactg
    15961 tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg
    16021 attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca
    16081 atcccggtgt tctggctggc gcttctgttg acactgtttt gttcactcac gctaggttgg
    16141 ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg
    16201 ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat gagcgcaatc
    16261 cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccgc
    16321 ctgatgcgta tcagcactat tgaagtgtat gaccagaact atgtaaaagc ggcggcgacc
    16381 cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc gttaccgccg
    16441 gttattcctc gcctgggctt acagttttcc accatgctga cgctggcgat gatcaccgaa
    16501 atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg ccagcaggat
    16561 tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat tgttaacgtg
    16621 atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg gtatgcctta
    16681 cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt
    16741 ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcgggactgg ctgtactgtg
    16801 tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg gttatcaatt
    16861 attgccgcca tcatggtcgc gctatggcga agtttctttc ttcctgggga ctgacgacct
    16921 cggacgtgat gttttaagcc gtttactgag cggagctgcg ccaaccgtgg gtggcgcatt
    16981 tgtggtgacg cttgccgcga cgatctgtgg cctggtactg ggcaccttcg ccggggcgac
    17041 acacggcctc cgctcagcgg tgcttaacca tattctggat accttactgg cgatcccttc
    17101 gctgctgttg gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc acgctatgtt
    17161 tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca tggtgcatga
    17221 cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat
    17281 tctctggttt gctgtgatgc caaacatcac cgccgggctg gtcaccgaga tcacccgcgc
    17341 actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg gcgcacaact
    17401 cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc
    17461 gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgttgttgg ttaacctcct
    17521 tggcgatggc gttcgtcgtg cgattattgc gggggtggaa taatgccatt actcgatatt
    17581 cgtaacctga ccattgaatt taaaaccggt gatgagtggg ttaaagccgt cgaccgggta
    17641 agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg ttccggcaag
    17701 agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt tactgctgac
    17761 cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg gcgcaaactg
    17821 gttggccata acgtgtcgat gattttccag gaaccgcagt cgtgtcttga cccttcagaa
    17881 cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg ccgttggtgg
    17941 cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa
    18001 gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga atgtcagaaa
    18061 gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgaca
    18121 aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa
    18181 aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag ccaatgggcg
    18241 gataaaatta acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag taaggagttg
    18301 gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc agacttcggc
    18361 agcgcgatgc cgcataaaag tcgcctcaat actctacccg gcgctatccc attgctggaa
    18421 cagttaccga ttggttgtcg tctggggcca cgttgcccgt atgcacaacg agaatgcatt
    18481 gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt cccgctgaac
    18541 atggagaaag agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt
    18601 ccgctaccgg accggttggt ttcgtcgtca gaccgtagaa gcggtaaaac ccttgagctt
    18661 tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg gtaaatccac
    18721 actggcaaaa atgctggcgg gaatgataga acctaccagc ggcgagttat tgattgacga
    18781 tcatccgctg cattttggcg attattcctt ccgtagtcag cgtattcgca tgatttttca
    18841 ggatccttcg acctcgttga atccccgtca acgtatctca caaattctgg attttccact
    18901 gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg
    18961 tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg cacccggaca
    19021 aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga
    19081 tgaagccctc gcttcactgg atatgtcaat gcgttcgcag ttgattaatc tgatgctgga
    19141 gttacaggaa aaacagggca tttcgtatat ttatgttacc cagcatatcg gaatgatgaa
    19201 gcacattagc gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc gaggcagcac
    19261 cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg ccgggcactt
    19321 tggcgaagca ttgacagcgg atgcgtggcg taaagatcgc taattttctg aatcctcctt
    19381 ccagattatt cacctcattc tgacaagaga ttaaatcttg tcagaatgag gtgatggata
    19441 aaataaaatc aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa cgaaagctct
    19501 ggcgatatct tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa catccggtgg
    19561 ggagctacat tctcgatttt gcttgctgct cggcgcgcgt agtcgttgag ctggatggtg
    19621 ggcagcatga tttggcagtt gcctatgata ccaggcgcac tagctggctt gagtcgcagg
    19681 gctggaccgt gctgcgtttc tggaataacg agattgattg taatgaggag gcggtgctgg
    19741 agattattct gcaggaactg aaccgccggt caccctcacc ctgaaagggc gagggggccg
    19801 accgagccga atagctgttg tggtgaaaac atggagacag tgctggagaa tattcggcaa
    19861 ggtctgaacc atcccagcca tcgccataaa agggttaggg gctgtatgag cctgtttgtt
    19921 gctggggtaa caatatttgc acaatatggt cccctcgccc ctctggggag agggttaggg
    19981 taaggggaaa agcgcccccc ctgccgcagc ctgctccggt cggacctggt aactatagct
    20041 actcacagcc aggttgatta taataaccgt ttatctgttc gtactgttta ctaaaacgac
    20101 gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc tatgggtttt
    20161 ctttccggta agcgcattct ggtaaccggt gttgccagca aactgtccat cgcctacggt
    20221 atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca gaacgacaaa
    20281 ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt tctgcagtgc
    20341 gatgttgcag aagatgccag catcgacacc atgttcgctg aactggggaa agtttggccg
    20401 aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct ggatggtgac
    20461 tatgttaacg ccgttacccg tgaaggcttc aaaattgccc acgacatcag ctcctacagc
    20521 ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc cctgctgacc
    20581 ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg tctggcaaaa
    20641 gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt
    20701 gttaacgcca tctctgctgg tccgatccgt actctggcgg cctccggtat caaagacttc
    20761 cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtacagt tactattgaa
    20821 gatgtgggta actctgcggc attcctgtgc tccgatctct ctgccggtat ctccggtgaa
    20881 gtagtccacg ttgacggcgg tttcagcatc gctgcaatga acgaactcga actgaaataa
    20941 tcgttctgtt ggtaaagatg ggcggcgttc tgccgcccgt tatctctgtt atacctttct
    21001 gatatttgtt atcgccgatc cgtctttctc cccttcccgc cttgcgtcag gataacgatt
    21061 tcctttacga ccaaggagcg cccatggaac aacgccacat caccggcaaa agccactggt
    21121 atcatgaaac gcaatccagt actacggagt atgacgttct gcctctggtc ccggaagccg
    21181 caaaggtcag cgatcccttt ctgctcgacg tgatccttga tgaagagacg ctggccccct
    21241 tcctttcatg gctggtccct gcgcgcgttc ttgcagtgga gttgttccct gaccagctta
    21301 ccgtgacccg ttcacaaacc ttcaccgctt atgaacgctt gtcgacggcc ctgacggttg
    21361 ctcaggtttg cggcgtccag cggttatgta actactattc ggcgcgactt acgccgctcc
    21421 ccggacctga ttcctccagg gaaagtaatc atcggttggc acaaatcacg caatatgccc
    21481 gccaactggc tagctcgcct tctattatcg acaaccgatc gcgccagcat ctgaatgacg
    21541 tcggtcttac tgtctgggac tgtgtgatca ttaaccaaat cattggtttt attggctttc
    21601 aggcgcggac aattgcgaca tttcaggctt atctcgggca tccggtacgc tggttacccg
    21661 ggctggcgat acaaaactac gccgacgcgt cactgtttgc tgatgaatca atacgctggc
    21721 gaagcagcta tgaggtggaa aaactacctg aagagcacac aaaaagttca actgcagaac
    21781 tttgccaact ggccgaaaca ctctctctcc accctatttc actttccctt ctcgaaaagt
    21841 tgttaaacag cacacgggtc aatgcacagc cggataatca gcttgcggcg ttgttatgcg
    21901 cgcgtataaa tggcagtcct gcttgttttt ccacctgtat ggattcatca aatgaatata
    21961 aaaaaatcag cacccttctg cgcaagggcg aaaatgaaat taaccgatgg gctgaccgtc
    22021 attctgttga gcgcgctacc gttcaggcga tacaatggct gacccgagca cccgatcgct
    22081 ttagcgccgc ccagttcagc cctttactcg aacacgaaaa atcatcaacg cagattatta
    22141 atctgctggt atggagcggg ctgtgtggct ggataaatcg cttaaaaatc gcgttgggtg
    22201 agacatatta accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc gctctaatgg
    22261 ccaccaaaat agacaattat gtttcaggac aacccgctgc tagcgcagct taaacagcaa
    22321 ctgcattccc agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa aggctttggc
    22381 ttcctggaag tcgacgcaca aaaaagttat ttcattccgc cgccgcagat gaaaaaagtc
    22441 atgcatggcg accgaattat cgcggtgatc cacagtgaaa aagaacgtga atccgcagag
    22501 ccagaagaac tggttgaacc gttcctgact cgtttcgtgg gtaaagttca gggcaaaaat
    22561 gaccgtctga ccatcgttcc tgatcatcca cttttaaaag acgccattcc ttgccgcgca
    22621 gcccgtggcc tgaaccacga gtttaaagaa ggcgactggg cggttgccga aatgcgccgt
    22681 catccgctga aaggcgatcg ttctttctat gctgaactga cacaatacat cacttttggt
    22741 gacgaccact ttgtaccgtg gtgggttacc cttgcgcgcc ataatctgga aaaagaagca
    22801 ccagacggcg tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga tctgacctcg
    22861 ctggattttg tcaccatcga cagtgccagc actgaagata tggatgacgc ccttttcgct
    22921 aaggcgctgc cggatgacaa acttcagctg attgtggcga ttgccgatcc aaccgcgtgg
    22981 attgctgaag gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac caactatctg
    23041 cctggcttca acatccctat gctgcctcgc gagctttctg acgatctctg ctcactgcgc
    23101 gccaatgaag tccgcccggt actggcatgc cgcatgacgc tctccgctga tggcaccatt
    23161 gaagataata tcgaattctt tgccgccacc atcgaatcca aagcgaagct ggtgtatgac
    23221 caggtttctg actggctgga aaataccggt gactggaagc ctgaaagtga agcaattgcc
    23281 gaacaagtcc gtttgctagc gcaaatttgc caacgccgcg gcgagtggcg tcataaccac
    23341 gcactggtgt ttaaagatcg cccggattac cgctttattc tcggtgaaaa aggtgaagtg
    23401 ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga agcgatgatt
    23461 gccgctaaca tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg catctataac
    23521 gtgcatatgg gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct gaaaacgcac
    23581 ggtctgcatg tcgatgccga agaagtgctc acgctggacg gtttctgcaa actgcgtcgt
    23641 gaactggatg cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt ccagtcattt
    23701 gctgaaatta gctctgaacc cggtcctcac tttggcctcg gtctggaagc atacgccacc
    23761 tggacttcgc cgatccgtaa atatggcgac atgatcaacc accgtctgct gaaagcggtt
    23821 atcaaaggcg aaactgcgac gcgtccgcag gatgaaatca ctgtccaaat ggccgaacgt
    23881 cgccgtctca accggatggc agaacgtgat gttggcgact ggttatacgc acgcttcctg
    23941 aaagataaag ccgggaccgg cacccgtttc gcggcggaaa ttgtcgatat cagccgtggc
    24001 ggcatgcgtg tgcgtttggt tgataacggc gctatcgcct ttattccggc acctttctta
    24061 cacgctgtgc gcgatgaact ggtttgcagc caggaaaacg gcactgtaca aattaaaggt
    24121 gaaacggttt acaaagtaac tgacgttatt gacgtcacca ttgccgaagt ccgcatggaa
    24181 acccgcagca ttattgcgcg cccggtcgcg taatctcctt tcacggccca ttcctcatgg
    24241 atgggccgtt tatttccccg ctctaccttc gttatatcca ggcagatttt ttaactgact
    24301 ttcgtttgaa aactggcgtt tttccaaaac tggattacaa ataaatatat aaaaaaacaa
    24361 tgcgtttagc cgccgttaat ttcatgtaag tcatgttgtt ccttttattt aaccgcggac
    24421 tccgctgtta accggaggat atgcatcatg aaaaccgtta gggagtccac aacgttgtac
    24481 aactttctcg gatcgcacaa tccatactgg cggttgacgg aaagcagcga tgctttgcgc
    24541 ttttctacca ccaaaaccac agaacctgat cgtacattgc agttatctgc cgaacaggct
    24601 gctcgcatca gggaaatgac ggtcatcacc tccagcctga tgatgagtct gaccgtcgat
    24661 gaaagcgatc tttctgtgca tctggtagga cgaaaaatca ataaacggga atgggctggc
    24721 aacgcgtctg catggcatga cacaccggcg gttgctcgtg atttatcaca cgggctttcc
    24781 tttgctgagc aggtagtttc tgaagcacat tccgcaatag tgattctcga cagccggggg
    24841 aatatccaac gcttcaatcg gttatgtgaa gattacacag ggttgaaaga acacgacgtc
    24901 attgggtaaa gcgtgtttaa actgtttatg agccgtcgtg aagctgcggc atccaggcgc
    24961 aataaccgtg tattttttcg aagcggcaat gcatatgaag tcgaactgtg gataccaaca
    25021 cgtaaaggcc agcggctgtt tctgtttcgc aataaatttg tccacagcgg cagtggcaaa
    25081 aacgagattt ttttaatctg ttccggtacc gacattaccg aagagcgccg cactcaggag
    25141 cgactgcgta ttctggcaaa taccgacagt atcaccggac tgccgaatcg taacgcaatg
    25201 caggagttaa tcgatcacgc tattaatcag gcagataaca ataaagttgg ggttgtgtat
    25261 cttgatttgg ataattttaa aaaggtcaac gacgcctatg ggcatttgtt tggtgaccag
    25321 ttattacgcg acgtgtcatt ggctatttta agctgtctcg aacatgacca ggtgttggcg
    25381 cgtccaggtg gcgatgagtt tctggtactg gcatccaata cctcacaaag cgcgctggaa
    25441 gcaatggcat cacgaatttt gacccgctta cgcctcccct ttcgtattgg tttaattgaa
    25501 gtttatacca gctgttcagt aggtatttcg ctctctcccg aacatggttc ggacagcgcg
    25561 actattattc gtcacgccga cacagcaatg tacacagcga aggaaggcgg acgaggacaa
    25621 ttttgcgttt ttactccaga aatgaatcag cgggtatttg aatatctctg gctggatacc
    25681 aacttgcgta aagcactgga aaacgatcag ttggttatcg aaaatcacct agcgtgtcga
    25741 agtgcgcagt ctggaagcac tagtacgttg gcagtcacct gaacgtgggt tgattccacc
    25801 gttggacttc atttcctacg ccgaagagtc agggctaatt gtgcctttag gccgttgggt
    25861 gattctcgat gtcgtacgcc aggtggcaaa gtggcgggat aaaggcataa acctgcgagt
    25921 ggcggtaaat atttctgcac gtcagctcgc cgatcaaacc attttcaccg ccctgaaaca
    25981 ggttctccag gaactcaatt ttgaatactg ccctatagat gttgaactga cagagagttg
    26041 tctgattgag aatgatgaac tggcactgtc tgttattcaa caatttagcc aactaggtgc
    26101 gcaagtgcat ctggatgatt ttggtaccgg ctactcttca ctttcgcaac tggcgcgctt
    26161 tccgatcgat gccatcaaac ttgaccaggt ttttgttcga gatattcaca aacaacctgt
    26221 ctcgcagtca ctggtccggg cgatcgtcgc tgtggcccag gcattgaatc ttcaggtgat
    26281 cgccgaaggt gtagagagtg caaaggaaga tgctttttta accaagaacg ggatcaatga
    26341 gcggcaagga tttttgtttg ccaaaccgat gcccgccgtc gccttcgaac gctggtataa
    26401 acgctatctg aagcgcgcat aagtagatga atatatatac ttagcgctat atcccgtcat
    26461 tatatatcgt attcctggat gataattagc cgttgctgtt aaacacttct cttttagcgg
    26521 caatttttgc attattcatt ttcccccttt tgctataagg atgtccttga tgtctgaatt
    26581 tgatgcccag cgcgttgccg aacgtattga tattgtgtta gatatccttg tggcaggcga
    26641 ttaccactct gcgatccata atctcgagat acttaaagca gaactcttgc gccaggttgc
    26701 tgaatctacg ccagacatcc cgaagacacc gtgggagatt taacaatcgt gccttcaggt
    26761 tttcctattt gaggatgccc ggcgcgtcag tattttttta tttagtatta taacgttata
    26821 agaattacag cgatgaattc ccgacaacaa actattctac agatggtcat tgaccagggt
    26881 caggttagcg taaccgatct ggcaaaagcc actggagttt ctgaagttac cattcgccag
    26941 gatctcaaca ccctcgaaaa actgagttac ctccgccgtg cacatggctt tgcagtttcg
    27001 cttgatagtg atgacgtcga aacccgtatg atgagcaact atacgctgaa gcgtgaactc
    27061 gccgagtttg ccgcgtcact ggttcaaccg ggcgaaacca tctttatcga aaatggcagc
    27121 agcaatgccc tgctcgcccg gactctgggg gagcagaaga aaaatgtcac tatcatcacg
    27181 gtcagcagct acatcgcgca tttgctgaaa gacgcgcctt gtgaagtcat tttgctcggt
    27241 ggcgtgtacc agaaaaaaag cgaaagtatg gttggccctt tgacacgcca gtgcatccag
    27301 caggtacatt tcagcaaagc atttattggt attgatggct ggcaacctga aactggattc
    27361 accggtcgcg atatgatgcg caccgatgtg gtcaatgccg tgctggaaaa agagtgcgaa
    27421 gcgatagtcc tgactgacag ctcgaaattt ggtgctgtac attcatactc catcggtccc
    27481 gttgagcgat tcaatcgcgt gattaccgat tcgaaaatac gcgccagcga tctgatgcat
    27541 cttgagcaca gtaaactcac cgttcacgtc gttgacattt aaccatttcc cttctctacg
    27601 gatgatttgc agtttggcaa atcatccgct ctaagatgat tcctggttga taattaagac
    27661 tatttacctg ttattaacac tctcaagata taaaattatt atcagcgata taacaggaag
    27721 taattatcac ctgcgtgata taaccctgcg cgcgagcaga tttcacggaa taatttcacc
    27781 aggcttattc ttagctatta tagttataga gagcttactt ccgtgaatca taaattcagg
    27841 agagagtatt atgtttgtaa cgagcaaaaa aatgaccgcg gctgttctgg caattacttt
    27901 ggcaatgtct ctgagtgcct gttctaactg gtctaaacgg gaccgcaaca ccgcaatcgg
    27961 cgcgggtgca ggggcattag gcggtgcagt actgaccgat ggcagtacgt tgggtacatt
    28021 aggtggtgca gctgtcggtg gtgttattgg tcaccaggtc ggtaaataat cgtaaataaa
    28081 tgtcgattat cgactcgcaa ttataatacg cttgattctg ttcagtacga aataactgaa
    28141 taatacacac agtaaaatat tcgaaagcca cggatatatc cgtggctttt tatgttaacc
    28201 gcctgcgagt tttaccttca tccctttcgc ttcaagtagt gattttaata aatcacgctt
    28261 atcgccctgg atttcaataa ctccatcttt aactgctccg ccgcagccgc attttttctt
    28321 cagttccgct gcaagttttg tcagttcggc atcatcgaga tcgataccgg taatcaggca
    28381 aacgccctta cctttacgtc cactggtctg acgctgaata cgcaccgctc catcgccttt
    28441 agggcgtact ggggccgctt tgggctcatc aatacgcccg gtttccgttg agtagaccag
    28501 acggctgttg gaatcactca tcatgcactc cgctgtaaag aggcgttgat cgctttcagt
    28561 gtctgcgctg gatctaccga ttgcgttacc gggcgaccaa tcaccatata atcaacacca
    28621 gccgccaacg cctgttctgg cgtcataatg cggcgctggt caccagcttc actcccctgc
    28681 ggacgaatgc ccggtgtaac cagtttgaac tcctgaccga atacctgttt aaagcgcaca
    28741 gcttcctgag cagaacacac cacaccatca aggtcacatt tttgcgtcag tgctgccaga
    28801 cgttctgcat aatctgcagg tgacagtgtc acgccaagat cggccaggtc gctggcttcc
    28861 atgctggtca acactgtcac agcaatcaaa agcggtgcat ctttgccaaa cggaaccagt
    28921 gcctcacgcg ctgcggtcat catacgcgcc ccaccggagg catgaacatt caccatccac
    28981 acgcctaagt cagctgcagc agcgacagcg tgcgctgcag tgttggggat atcgtggaat
    29041 ttcaggtcaa gaaagatatc aaaaccacgc tgttgaagtt cgcgcacaaa ctgtggccca
    29101 aacaatgtaa acatctcttt accgaccttc agacgacaat cgcgtgggtc gatcttgtcg
    29161 acaaaggaca gcgcgtcatc acaattatga taatcaaggg caacaaccac aggagaattc
    29221 gtaacggcgc gggaagaaga tgaagcagtt aacgtcatga ccagaccttc ttgatgatgg
    29281 gcaccgggag tggcgcgaaa caggtaaacg gcgagcattc tacccgtgaa aataaaaaat
    29341 taacagttgc gatttttccc ctagcaggct ttttctgctc cgtcgttgcc ggagcacaat
    29401 gggacatcaa taactagtat gttgtaacta aagtcggatt tttttaaaaa ttacaggcca
    29461 tcaagaccgc gaatcggttt aatagttgac caggcacgac aagacggaca atgccagtag
    29521 agggtgtatg cggtaaaacc acatttctgg cagctataac gaggcttact acgcaccttc
    29581 tcgccaacca tgtcacgcag caccatcaga ctctctttgg cgcgcccttc ttccgcttca
    29641 tttaagtggt aatccattag cttatggaat acacgcatgg tcggatgacg ctgaagctgg
    29701 cgtgtaatat agacctgtgc agcctcacta ccgtcgcgcg cttcgatgat atcagcaagc
    29761 atcaattcag catcggcacc ggtgttctct tccaccgcgc gttgcaggaa ttctgcccat
    29821 tcggcagttt tacccaactg ctgatagcag gtttgcaaca tttccagcgt ttcgctgacc
    29881 agttctctgt cctgggatat gacgcgttgc aaactttcga cggctttggc gtattctcct
    29941 ttcgccataa acacgcgtcc catcatgatg gatacgcggg cgctgttttt atctgccgcc
    30001 gccccttttt ttagcaaggt catggcacga tcgagatcgt cgctggccat atgctgcagg
    30061 gctaactcac agtagaaatg ggcaatttcg acgcgctgtt tatctttacc cagcttcacc
    30121 aggcgttcgg caacatcaat tgctttctgc cactcactgg tagcctggta gatttgtagc
    30181 aactgttgca gcgcgccaat gcggaagtca gtttcatcgg tcagctgatt gaacatgtct
    30241 tccgcgcggt cgtataaccc ggcggccatg taatcacgcc ccagttgttg aatcgccaac
    30301 agacgctgtt cataggtcag cgaggcgctt tccattaggg tctgatggat gcgaatagcg
    30361 cgatcaactt cgccacgcga acggaacagg tttccgagcg taaggtgggc ttcaacggtg
    30421 cctgtatcct ctttaagcat atcgagaaac aggtctaccg ctttatcctg ttgattacta
    30481 agcaggaagt taacccccgc tacgtaatca cgcgacaagc ggttggcttc atcttgcttg
    30541 ttttgttgcg cacttctgcg gcccatatac cagccatagg cagcggctac aggcaaaagc
    30601 agaaacaaca actccagcat agaaagttat tccttcgccg ctgacgagtg cggcactaca
    30661 gccacgtcag tcgcgggtga aagctggttt tccagtcgct ttattttacg ttcagcgcgc
    30721 gccagggaaa cacgaactcg cagccagaac aggccacaaa tcaaccaacc gatagcaaac
    30781 cccgcagcaa acaataccgc cagcaatgtg gaaatacggt actccccttg cgctaacaga
    30841 taattaaacg tcacctgttg atcgttctgc gcacccaacg tgaccgaaat cacgaagatc
    30901 gctaacacca gtaagaaaat gagtaaatat ttcacattac ttcccgttat gtggttagag
    30961 cgaataaatt atggtcaact taccgcaata gccctttaaa gtaccatttt cgcgccgtct
    31021 gcgaaatggc atccacgcgc attgttgatg atttaaggcc taaattcgga aaatcaacca
    31081 gcgttaactt tcttgttctc gttgtgcaat ttcgcgattt tcttccgcag gtggtgttaa
    31141 tggcccacaa attcgttgcg caagccacgt tgccaccgcc accagcgccc acgaaatcaa
    31201 cgtagctacc accagatcgc gtggccaatg catcccgagc agcaggcggc ttcccatgac
    31261 tcccgttgcc cagaccaaca agatagcaat ggttaacgtt cgccgacgcg gccacagcaa
    31321 accaacggcc agcagtgccc aactggcagc aaacatcgtg tgaccggaag gaaaggcaaa
    31381 ccccgtctct ttctgccagt gtgaacgcaa atattgtgga atatttttct cttcagccaa
    31441 ctgttctttt actagatttc cgcgttctgc tcgctttaaa gtgtagaact catcaaccgg
    31501 aatatgatgt gttttttcca accagataac aaaaggtcgt ggttcctgta ctttgtcttt
    31561 gatccaggat ttaacgcctt gtccaacaag gattgccgcc gccagaatgg caaataatac
    31621 aaaggcagcc ttaatgcgaa aacgcagaca ccagagaaac cagccgaaca aaatcaaatg
    31681 tgtaatgacg ccccagggct gggtgacagt ttcagtaacc caaaaagccg cttttagtag
    31741 ccaactttgt tctccaggtt gccaacgcca gccagaaatc catacggcta ctggcatgac
    31801 aagcaatagt gcagctccca ctgcggtacg tcgggcaatc gaacgcatgg cctctccttt
    31861 ttgataagtc ccacaatcat aactgaaaat gccagttcca agaaaaattg acagatttgt
    31921 gccattccgt gaacgatcga cgcgtcgtga ttaggtgaac cccttctcgt tatggcaaaa
    31981 taagccaata cagaaccagc attatctgga gaatttcatg cagcttaaac gtgtggcaga
    32041 agccaaactg ccaaccccat ggggcgattt cctgatggtg ggatttgaag aactggcaac
    32101 cggacacgat catgttgcgc tagtctatgg cgatatttcc gggcataccc cggtacttgc
    32161 gcgcgtccat tccgaatgtc tgaccggtga cgccctgttc agcttgcgct gcgattgtgg
    32221 cttccagctc gaagcggcat tgacgcaaat tgccgaggaa ggccgtggta ttttgctgta
    32281 tcaccgtcag gaaggtcgta acattggtct gctgaataaa atccgcgctt acgcactgca
    32341 ggatcaaggt tacgataccg tagaggctaa ccaccagtta ggcttcgccg ctgatgagcg
    32401 cgacttcact ctttgcgctg atatgttcaa actccttggc gtcaatgaag tccgcttgtt
    32461 aaccaataac ccgaaaaaag tcgaaattct gaccgaagca gggattaata ttgttgaacg
    32521 cgtaccattg attgtaggtc gtaaccccaa taacgaacat tatctcgata ccaaagccga
    32581 gaaaatgggc catttgctga acaaataacc ctcttgcatc gtgtaattca tttgcttgcc
    32641 ggaagcaaaa taaccggcaa gcaaatactt gttacttcaa catattacga atgacataat
    32701 gcaaaatgcc gtcgttctgg tagtaggtca actccgtcgc ggtgtcgata cgacaacggc
    32761 agggtacgac ttcctggcta ccatccgcgc gcgtaagcgt caccggaacc gtcgcgccag
    32821 gttgtagatt ttgcagatcg ccaatatcaa tcttctcttc cccggttagc cctaacgttt
    32881 tacgcgtcac gccttgcgga aattccagcg gcaggatgcc catgccaatt aaattcgaac
    32941 ggtgaattcg ttcaaacgat tcggcaatca ccacacgaat tccaagcaga cgaggtcctt
    33001 ttgccgccca gtcacggctt gagcctgatc catactcttt cccggcaatc accgccagcg
    33061 gcgtttgctc ctgcttatag cgcatcgcag catcataaat agagactacg tcgctgtcag
    33121 gtaaatgccg cgtcatcccc ccttcaacgc caggcaccat ttcattacgg atgcgaatat
    33181 tggcgaaggt gccgcgcatc atcacttcat ggttaccacg ccgcgaaccg taggagttaa
    33241 agtcttttcg ctcaacaccc cgaccttgta gatatcgacc cgctgggctg tcgggcttaa
    33301 tactgcccgc cggagagata tggtcagtgg tgactgaatc ccccagcatt gcgaggatcc
    33361 gcgcaccgtg aatatcttcc actggtgctg gtgttgcctg catttcatca aagaaaggcg
    33421 ataagcgaat ataggttgag tcctcctgcc aaccgtaggt atcggatcgt gtgacgttaa
    33481 tttccttcca ctctgctgtg ccttcaaaaa cttctgcgta ctctttgcgg aacatttctg
    33541 tggagacttg ttctaccgca cgggcaattt cttgtgccga tggccagata tctttcagat
    33601 aaaccggctc gcctttgcga tcatggccga taggctcaga agccaggttg atattcatat
    33661 ttcccgccag cgcataggca accaccagcg gtggcgaggc cagccagtta gttttaacca
    33721 gcggatggat acggccttca aagttacggt tgccggacag caccgcaccg acggttaaat
    33781 cgcctttttt gattgccgtt tcgataggat cgggcagcgg cccagagtta ccaatacagg
    33841 tggtacaacc gtatcccaca aggttaaacc ccagttcgtc gagatacggt gtcagtttcg
    33901 cttttgccag ataatcagaa acgactttcg aacccggtgc caacgacgct ttgacccacg
    33961 gttgccgctt gaggccaaga gttacggctt ttttcgccag caagcctgcg gccatcagca
    34021 cacttgggtt agaggtgttg gtgcacgagg ttatcgcagc aatgaccaca gcgccatcag
    34081 gtaactgata ctgatgtccg ttcataacat aatcgactgg caggcgatct ttatgcgtgg
    34141 cattcacttt cagttcgtta ctggcggcaa atgcttttgg tacatcgggc agtgcaacgc
    34201 gatcctgtgg gcgttttggc cctgccaggc tcgcttcaac gtcattcata tccagttcta
    34261 acatactggt aaaaatcggt tcatcgcccg ggttacgcca catgccctgc gctttggcat
    34321 atttttcgac caactcgacc tgatcttcgc tgcgcccgct taaacgcatg taatcgaggg
    34381 ttactgcatc gattgggaag aagccacagg tggcaccata ttctggcgac atattggcaa
    34441 tggtggcgcg atccgccaac ggtagtgaat ccagaccatc accataaaat tcgacgaatt
    34501 tccccaccac gccatgtttg cgcagcattt gggtaacagt gagaaccagg tctgtggcgg
    34561 taataccttc acgtaatttt ccggtaagtt taaagcccac tacgtccggg ataagcatgg
    34621 aaaccggctg gcctaacatg gcggcttctg cttcgatccc acccacgccc caccccagca
    34681 cgccaaggcc gttgatcata gtggtgtgcg agtcagtacc aacgagtgta tccggataag
    34741 caatccattc accgtcctgc aattcgctcc acactgcttt gccgagatat tcgaggttaa
    34801 cctgatggca aatgcctgtg cctggcggca cgacgctaaa ccgactgaac gcttgctttc
    34861 cccatttcag gaacacataa cgttcgtggt tgcgctccat ttccaggcgt acgttttctt
    34921 caaatgcctc atcatcacca aaacgatcga cggtcaccga gtggtcaatg accaggtcga
    34981 ccggtgagag cgggttaacc tttgcagtat cgccaccgag gcgtttaacc gcttcgcgca
    35041 ttgccgccag atcaacaacg gcaggtacgc cggtaaagtc ctgcatcagc acccttgccg
    35101 ggcggtaggc aatttcacgg tcagcatggg catttttcag ccatcccgcc agtgcgtgga
    35161 tatcctcttc ggtaactgag ttaccatcct gccagcgcag caggttttcg agcaaaactt
    35221 tgagtgactt gggtagacgg gtgatatcgc ccagtgattt agcagcaagc ggcaggctgt
    35281 agtagtggta agttttatct ttggcctgca acgtgtcctt actggcttct cgtagggttg
    35341 acgacatagc tcctccttaa tgacagggtt gcgaagccct gattctcttc agggtgaata
    35401 ttaaagataa cacaaacatc gcgtaacgat ttgataacaa cccaaattga taaaagagga
    35461 gaaaccttgt ggcaaagcaa atgacaaccc cgccgcagcg gggtcaagga tcagtgagta
    35521 atgatccaga ggagttcggc ccagaagagc atcgacagag aaaacgcgcc catccaggac
    35581 caatatttca tatttgtgtt gatcataggc tacaccctta acggttatta ttttgtgttg
    35641 agataactca agctgttatt gctgcggtgt attaaaagcc ctttaattca ttaaaaatgg
    35701 gtaataagaa tgatgataat tattgtttcg gctgatcatc ttcagcaaac agttctataa
    35761 aggcttgctg ctgcgatgtt agttgccagt cgtctggaat aacgacggaa cattcccgtt
    35821 tcgtttctgg ttcattgtca ctatgctggc ataatggtga cgactccagt tgctcctgac
    35881 cgaccatagt tatccccaca ctttccaaat cagcagtgcg acaaccaccc agaaaagtgc
    35941 agaaccaaga aaaaccgcca gccaggcttt acgttttaat tcaggatcac ggcggggttc
    36001 ctgatttcca gaaggcattg ctaacctcat ataatcgcca tcacttatca gcaagacgtt
    36061 gaatgattgg tgtaattaat catacgatga gacaaatcct aaagaaactt tagtcaaaaa
    36121 tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct gaaatagata
    36181 tttgaaaact ggaatttgcg aggcgggtaa ttagacacta ctttacaggt aatttaccct
    36241 ataaatatga gggataaaaa tattgtgcac ggtatagaaa taagtaacaa aagagaatat
    36301 attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt atttttccgg
    36361 cagttttata tctttaaaca tgacctcaat ttcttcattg gagcgcaatg cgacagccgc
    36421 atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat acatataact
    36481 gcgcaaaaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga agatatcgtg
    36541 agcatcaacg cgcacaaggt cgggatcggc gaccggatcc accgccatgc tggcaatgac
    36601 ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg ttgccgtgaa
    36661 aacgatacgc ggcgttaacc ctgcgcggtt aaaggcagta tccagttctg aacgtccggt
    36721 aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg taatggcttt
    36781 tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt ggtagcacgg
    36841 taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagcga aatcggcatt
    36901 gcctttagag acggcatcag caatttgtgt cggcgggccc tggtgcatat gcaaagaaac
    36961 gcggggataa cgctcaataa agcctttgat aacgtttggt aacgcgtagc gtgcctgggt
    37021 atgcgtggtg gcgatataca gcgagccttt atccggccag gtgtgctcac cggcaaccga
    37081 ttttatggca tcgactttcg acaggacttc gcgagcgata cgaattattt cttgtcctgc
    37141 tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc ctagctcgtc
    37201 ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc cttccgctgt
    37261 tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt gttgtaattt
    37321 catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac acctggcggg
    37381 tgactaatac gatttaataa taaaaaaggt gctaactata accactatat catttatagc
    37441 cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc tttcgcgacc
    37501 ctttgtttat aaaaacctga cagaattaaa ggttattttt ttccttcaac ccatttgcca
    37561 tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga gacatattgc
    37621 tgtttggttt tacggctaaa gcgaaccatg gtcttattac cttccggatc ctgctgtggc
    37681 gcatcggcca gataacgcag tttttccggc agacggtcgc ggaagcgata aagctcttcc
    37741 accagtggcg cacgcgtttc acgcgatttc gggaaagtgt tggcagccag gaacacaccg
    37801 gcagcaccgt cacgcagcac gaaataagca tctgattttt cgcacggcag ctcaggtaat
    37861 ggcaccggat cttctttcgg tggtgccact tcaccgttac gtaaaatctt acgtgtgttt
    37921 ttacactctt cgttggtgca ggccatgtat ttaccgaaac gccccatttt caggtgcatt
    37981 tcagagccac atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattcg
    38041 ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg caacttacgt
    38101 ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tcgggcaacg acgttttgcg
    38161 cgcagcgcgt tggtttcagc atcttcgcct tccagcacgt tcagcacttc gttttccggc
    38221 accaggttaa tggtggtttt gcaacgctct ttcggcggca gcgcatagcc agaacagcca
    38281 aggaataccc cggtactcgc tgtgcgaatc cccattttgc gaccacaagt cgggcagtca
    38341 atgctggtca gaaccatctg gttcgggcgc ataccaccct cttccggatc tttttcagct
    38401 ttatctaact gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct
    38461 tcgtgatttg ccacctggtc gaggctgttt tccatctgcg cggtaaagtc gtagttcatt
    38521 aactcgcgga aattttcttc aaggcgatcg gtgacgattt cgcccatttt ttccgcatag
    38581 aaacgacgat tttctactcg cacatagcca cgatcctgaa tggtcgaaat gatcgacgca
    38641 taggtagacg gacgaccgat accacgtttt tccagttctt taaccagcga tgcttcactg
    38701 aaacgggctg gcggcttggt aaagtgctgg gctggtgtta gttcaacgag cgtcagagca
    38761 tcgcctttat cgactgccgg taagatacga tcttcatcgc ctttacgcag tgcaggcatc
    38821 acttttgtcc agccatcaaa gcgcaaaata cgaccgcgtg ctttcaggcg gaaatcgccc
    38881 gcaccaacgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggcaacgaac
    38941 tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt cagcgattcc
    39001 gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg
    39061 ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa
    39121 ccgcgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg catgtaagtg
    39181 atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaaacca
    39241 agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg
    39301 gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc
    39361 gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcattctg atgagtcacc
    39421 tgcaactcca acgcttcacc agatggcgta gtcgtgctgg catcgacttc ccagaactct
    39481 tccggtacga acgctttaat ttcacgctca cgctctacca ccaggcggac cgccaccgac
    39541 tgcacacgac cggcagacag accacgagcg atctttttcc acagcagcgg cgaaaccata
    39601 taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc
    39661 agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc
    39721 actcggctat agcgcgcatc atcaccacca atcacttccc gcaggtgcca tgcaatggct
    39781 tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt
    39841 ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg
    39901 tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt
    39961 ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc actggtcggc
    40021 aaatcgcgga tgtgaccgac gctggatttc accacgtagt cacaacccag atacttgttg
    40081 atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta
    40141 cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt
    40201 taccgatatg gactcgctct ctgcggatat caaccccttg tcgtgactct gttcacgaaa
    40261 attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggatttttg
    40321 gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa
    40381 atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc
    40441 accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttgtttaag
    40501 gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt gaaagaggcc
    40561 aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg
    40621 cagcgtaatg gcgtgctggt atttaccggc gactactttt tagatgaaca aggattgcca
    40681 acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa
    40741 aaatatcatc tggttgatta aagcaaaacg cgaggtctta gcctcgcgtt tgtcttttac
    40801 atcaatggct tttgaccccg ctgccaccag cgtagcaaca atcgatcggc gctctctgcc
    40861 gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac attaaccact
    40921 tcacggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg
    40981 cctttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg ttcaatatcc
    41041 agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc gttcagctct
    41101 tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag cgtgcgctta
    41161 tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa gcggttaaag
    41221 ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt
    41281 ttgtccgcca cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca
    41341 gttaaaggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa cccgtaacca
    41401 tgcaccatgc caccagggct ttccagacgg agcacaacct gatcctgcgg tttgaatgct
    41461 gcgagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct
    41521 ttaaaatcca gcacccagac gcgaggttta ctgtcagttg ccacctcgcc cagtttggct
    41581 ttcgcttttg ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt gtgccactgt
    41641 ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc cttatactgt
    41701 tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg agcaacattg
    41761 acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt agccaaaaac
    41821 aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc
    41881 acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc gaaagtatga
    41941 caaagtatcc attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag
    42001 gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag
    42061 tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg
    42121 gagccagcga tgggattggt cgtgaagccg cgatgacgta tgcacgctat ggtgcgacag
    42181 tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag
    42241 aaactgggcg tcagccacag tggtttattc tcgatttgct gacctgcacg tccgaaaatt
    42301 gccaacaact ggcacagcgc attgtcgtta attatccgcg tctggatggt gttttgcata
    42361 atgccggatt gctcggcgat gtttgcccaa tgagcgaaca aaatccgcag gtctggcagg
    42421 acgtcatgca gatcaacgtt aatgccacct ttatgctcac ccaggcactg cttcctttat
    42481 tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttgga cgtcagggac
    42541 gagccaactg gggtgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac
    42601 tggccgatga atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca
    42661 ccgcaatgcg cgcaagcgcc ttcccgaccg aagatccaca gaaacttaaa acacccgctg
    42721 atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga
    42781 cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca
    42841 acagcgtcag cagcgagtga aagaaaaagt agatgctcgt gtggcccagg cccaggatga
    42901 acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg
    42961 tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg
    43021 cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt ttcaggtgat
    43081 ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg cctgccgcga
    43141 agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatatgg ttttgcttga
    43201 tgaactgacg tatatggtgg cgtatgacta tttgccactg gaagaagtgg tgcaggcgtt
    43261 aaatgaacgt ccacatcaac agacggtgat tatcacgggt cgaggctgtc atcgggatat
    43321 tcttgaactg gcggatacgg taagtgagct gcgccccatc agacatgcgt ttgatgccgg
    43381 tgttaaggcg caaataggga ttgattatta atctgtaaaa atgccggata tgacgtccgg
    43441 catactttga ttaaccgtta ttacgcccgc cagaacgacg actgccgctc acctgactgt
    43501 gacgtttcac cgcacgacga atctgattag ccttcatgcg acgacggtct ttttctacag
    43561 cgactttaga gctggtttct ggcggaagct ccaccagttc gcgcagatag ttagtctggg
    43621 cgaggtccag ctccgtccag ccaccgcgtg gcagaccttt tggcagtggg atatcaccgt
    43681 aacgaacacg gatcaggcgg ctcacctgca caccaaccgc ttcccacaga cgacgaactt
    43741 cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct tcgccgccgc
    43801 tgaacttgat ggttttaaaa gctgccggac catcttccag ttgcacgcca cgactcaaat
    43861 cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcgtattca cgttcaactt
    43921 cacggcttgg atgcattaaa cggtttgcca gttcaccatc ggtggtgaac agcagcagac
    43981 cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcgccacgc agctttggca
    44041 gacggtcaaa caccgtcgga cgcccttccg gatcgttacg ggtacacaac tcaccttccg
    44101 gtttgtaata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc gaaatcagat
    44161 gaccatcgat acgaattttc agcccagggg taacttcaac gcgatcgccg agtttagcaa
    44221 ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca cggcgggagc
    44281 cgtggccagc gcgggccagc actttctgta atttttcgct catagagctt ccttttggtg
    44341 tcgccttcac aggcgtcgaa caggagaatc aatggcgccg tatagcgcca ttttaaggcc
    44401 gcgtagtata cagagttacg cacttataag aaaggcttca catcacctac gccttcacgc
    44461 accacgaccg gcgtatcatc ggtaagatca ataaccgttg ttggtttctg cccgagatag
    44521 ccgccgtgaa taatcaaatc tacctgtttt tccagacggt ctttaatttc ttcgggatcg
    44581 gattcggtaa attcgctgcc tggcagcatt agcgaagtgg aaagcatcgg ttcgcccagt
    44641 gcttcaagca acgcctgcgc aatcgggtta gaaggcacac gcataccgat ggttttgcgt
    44701 ttctcctgca acagacggcg tggcacttcc ttcgtcccct tcaggatgaa ggtatagttg
    44761 cccggcgtgt tgtttttcat caaacgaaac gcaacgttat ccacgaatga ataggtcgag
    44821 agttcagaaa gatcgcgaca catcagggta aagttgtgac cgtccggcag ctggcgaata
    44881 cgacaaatac gctccatggc gtttttatct tcaattttac agccgagcgc atagccggaa
    44941 tcagttggat agacaatcac cccgccttta cgcacgatct ccaccgcctg gttgatcaga
    45001 cgttgctgcg ggttatcagg atgaatataa aaaaactggc tcataattcc ctctctgtgg
    45061 tgttctgcgg ctgttcccat agctgccaga cgccttcaac gcctgcgggc agccagagtt
    45121 tacgacccag ttcgatccac gggcatggct gatgaaaatc agatccttgt gatgcccata
    45181 aatgatgctg acgcgcaagt gtggccagct gggtgcgttc attgggcgat tgctggcact
    45241 gcgcgacttc catcgcgtcg ccgtggtgtt cggcaaaatg cgcaaccaat cttttcagcc
    45301 atttagcact aagattgtac cgcccaggat gagctagcac cgccttaccg ccagaatgat
    45361 gaatgacatc aatagcttgt tctattgtac accactgtgg cggaacgtat ccggttttcc
    45421 cgcgcgccag atacttttta aagacatctg ccattgaact ggctttgccg cactccacaa
    45481 ggaaacgcgc aaaatgaccg cgcgtcactg cgccgccctg cgccagtcgt tgtgcgcctt
    45541 ccagcgcccc aggtatttgc gctttttcaa gtctttcggc aatttgctgc gcccgctgat
    45601 tgcgccgttc tgtctgctgc gcgaggaact cacacattag cggatgagta atatcaatat
    45661 tcagcccgac aatatgaatt tcatgatttt cccagaccgt ggaaatttcc acgccgggaa
    45721 taagattcag cgccagtccc gaacgtgaaa tttcctctct tgctggcgcg attgcagctg
    45781 tggtgtcatg gtcggtgatc gccagggtgc cgacgcgcat ctcgactgca cggtgcacca
    45841 atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca
    45901 ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac
    45961 atcataacgg gtctggcaaa tattctgaaa tgagctgttg acaattaatc atcgaaccag
    46021 ttaactagta cgcaagttca cgtaaaaagg gtatcgacaa tgaaagcaat tttcgtactg
    46081 aaaggttggt ggcgcacttc ctgaaacggg cagtgtattc accatgcgta aagcaatcag
    46141 atacccagcc cgcctaatga gcgggctttt ttttgaacaa aattagagaa taacaatgca
    46201 aacacaaaaa ccgactctcg aactgctaac ctgcgaaggc gcttatcgcg acaacccgac
    46261 tgcgcttttt caccagttgt gtgggaatcg tccggcaacg ctgctgctgg aatccgcaga
    46321 tatcgacagc aaagatgatt taaaaagcct gctgctgata gacagtgcgc tgcgcattac
    46381 agcattaggt gacaccgtca caattcaggc actttccggc aatggcgaag ccctgctggc
    46441 actactggat aacgccctgc ctgcgggtgt ggaaagtgaa caatcaccaa actgccgtgt
    46501 gctgcgcttc ccccctgtca gtccactgct ggatgaagac gcccgcttat gctccctttc
    46561 ggtttttgac gctttccgtt tattgcagaa tctgttgaat gtaccgaagg aagaacgaga
    46621 agcaatgttc ttcggcggcc tgttctctta tgaccttgtg gcgggatttg aagatttacc
    46681 gcaactgtca gcggaaaata actgccctga tttctgtttt tatctcgctg aaacgctgat
    46741 ggtgattgac catcagaaaa aaagcacccg tattcaggcc agcctgtttg ctccgaatga
    46801 agaagaaaaa caacgtctca ctgctcgcct gaacgaacta cgtcagcaac tgaccgaagc
    46861 cgcgccgccg ctgccagtgg tttccgtgcc gcatatgcgt tgtgaatgta atcagagcga
    46921 tgaagagttc ggtggcgtag tgcgtttgtt gcaaaaagcg attcgcgctg gagaaatttt
    46981 ccaggtggtg ccatctcgcc gtttctctct gccctgcccg tcaccgctgg cggcctatta
    47041 cgtgctgaaa aagagtaatc ccagcccgta catgtttttt atgcaggata atgatttcac
    47101 cctatttggc gcgtcgccgg aaagctcgct caagtatgat gccaccagcc gccagattga
    47161 gatctacccg attgccggaa cacgcccacg cggtcgtcgc gccgatggtt cactggacag
    47221 agatctcgac agccgtattg aactggaaat gcgtactgac cataaagagc tgtctgaaca
    47281 tctgatgctg gttgatctcg cccgtaatga tctggcacgc atttgcaccc ctggcagccg
    47341 ctacatcgcc gatctcacca aagttgaccg ttattcctat gtgatgcacc tcgtctctcg
    47401 cgtagtcggc gaactgcgtc acgatcttga cgccctgcac gcttatcgcg cctgtatgaa
    47461 tatggggacg ttaagcggtg cgccgaaagt acgcgctatg cagttaattg ccgaggcgga
    47521 aggtcgtcgc cgcggcagct acggcggcgc ggtaggttat ttcaccgcgc atggcgatct
    47581 cgacacctgc attgtgatcc gctcggcgct ggtggaaaac ggtatcgcca ccgtgcaagc
    47641 gggtgctggt gtagtccttg attctgttcc gcagtcggaa gccgacgaaa cccgtaacaa
    47701 agcccgcgct gtactgcgcg ctattgccac cgcgcatcat gcacaggaga ctttctgatg
    47761 gctgacattc tgctgctcga taatatcgac tcttttacgt acaacctggc agatcagttg
    47821 cgcagcaatg gtcataacgt ggtgatttac cgcaaccata ttccggcgca gaccttaatt
    47881 gaacgcctgg cgacgatgag caatccggta ctgatgcttt ctcctggccc cggtgtgccg
    47941 agcgaagccg gttgtatgcc ggaactcctc acccgcttgc gtggcaagct gcccattatt
    48001 ggcatttgcc tcggacatca ggcaattgtc gaagcttacg ggggctatgt cggtcaggcg
    48061 ggcgaaattc ttcacggtaa agcgtcgagc attgaacatg acggtcaggc gatgtttgcc
    48121 ggattaacaa acccgctgcc ggtggcgcgt tatcactcgc tggttggcag taacattccg
    48181 gccggtttaa ccatcaacgc ccattttaat ggcatggtga tggcagtacg tcacgatgcg
    48241 gatcgcgttt gtggattcca gttccatccg gaatccattc tcaccaccca gggcgctcgc
    48301 ctgctggaac aaacgctggc ctgggcgcag cagaaactag agccagccaa cacgctgcaa
    48361 ccgattctgg aaaaactgta tcaggcgcag acgcttagcc aacaagaaag ccaccagctg
    48421 ttttcagcgg tggtgcgtgg cgagctgaag ccggaacaac tggcggcggc gctggtgagc
    48481 atgaaaattc gcggtgagca cccgaacgag atcgccgggg cagcaaccgc gctactggaa
    48541 aacgcagcgc cgttcccgcg cccggattat ctgtttgctg atatcgtcgg tactggcggt
    48601 gacggcagca acagtatcaa tatttctacc gccagtgcgt ttgtcgccgc ggcctgtggg
    48661 ctgaaagtgg cgaaacacgg taaccgcagc gtctccagta aatccggctc gtcggatctg
    48721 ctggcggcat tcggtattaa tcttgatatg aacgccaata aatcgcgcca ggcgctggac
    48781 gagttaggcg tatgtttcct ctttgcgccg aaatatcaca ccggattccg ccacgcgatg
    48841 ccggttcgcc agcaactgaa aacccgcacc ctgttcaatg tgctggggcc attgattaac
    48901 ccggcgcatc cgccgctggc gttaattggt gtttatagtc cggaactggt gctgccgatt
    48961 gccgaaacct tgcgcgtgct ggggtatcaa cgcgcggcgg tggtgcacag cggcgggatg
    49021 gatgaagttt cattacacgc gccgacaatc gttgccgagc tgcatgacgg caaaattaag
    49081 agctatcaat tgaccgctga agattttggc ctgactccct accaccagga gcaactggca
    49141 ggcggaacac ctgaagaaaa ccgtgacatt ttaacacgct tgttacaagg taaaggcgac
    49201 gccgcccatg aagcagccgt cgctgcgaac gtcgccatgt taatgcgcct gcatggccat
    49261 gaagatctgc aagccaatgc gcaaaccgtt cttgaggtac tgcgcagtgg ttccgcttac
    49321 gacagagtta ccgcgctggc ggcacgaggg taaatgatgc aaaccgtttt agcgaaaatc
    49381 gtcgcagaca aggcgatttg ggtagaaacc cgcaaacagc agcaaccgct ggccagtttt
    49441 cagaatgagg ttcagccgag cacacgacat ttttatgatg cgctacaggg tgcacgcacg
    49501 gcgtttattc tggagtgtaa aaaagcgtcg ccgtcaaaag gcgtgatccg tgatgatttc
    49561 gatccggcac gcattgccgc catttataaa cattacgctt cggcaatttc ggtgctgact
    49621 gatgagaaat attttcaggg gagctttgat ttcctcctca tcgtcagcca aatcgccccg
    49681 cagccgattt tatgtaaaga cttcattatc gacccttacc agatctatct ggcgcgctat
    49741 taccaggccg atgcctgctt attaatgctt tcagtactgg atgacgacca atatcgccag
    49801 cttgcagccg tcgctcacag tctggagatg ggggtgctga ccgaagtcag taatgaagag
    49861 gaactggagc gcgccattgc attaggggca aaggtcgttg gcatcaacaa ccgcgatctg
    49921 cgtgatttgt cgattgatct caaccgtacc cgcgagcttg cgccgaaact ggggcacaac
    49981 gtaacggtaa tcagcgaatc cggcatcaat acttacgctc aggtgcgcga gttaagccac
    50041 ttcgctaacg gttttctgat tggttcggcg ttgatggccc atgacgattt gcacgccgcc
    50101 gtgcgccggg tgttgctggg tgagaataaa gtatgtggcc tgacgcgtgg gcaagatgct
    50161 aaagcagctt atgacgcggg cgcgatttac ggtgggttga tttttgttgc gacatcaccg
    50221 cgttgcgtca acgttgaaca ggcgcaggaa gtgatggctg cggcaccgtt gcagtatgtt
    50281 ggcgtgttcc gcaatcacga tattgccgat gtggtggaca aagctaaggt gttatcgctg
    50341 gcggcagtgc aactgcatgg caatgaagat cagctgtata tcgatacgct gcgtgaagct
    50401 ctgccagcac atgttgccat ctggaaagca ttaagcgtcg gtgaaaccct gcccgcccgc
    50461 gagtttcagc acgttgataa atatgtttta gacaacggcc agggtggaag cgggcaacgt
    50521 tttgactggt cactattaaa tggtcaatcg cttggcaacg ttctgctggc ggggggctta
    50581 ggcgcagata actgcgtgga agcggcacaa accggctgcg ccggacttga ttttaattct
    50641 gctgtagagt cgcaaccggg catcaaagac gcacgtcttt tggcctcggt tttccagacg
    50701 ctgcgcgcat attaaggaaa ggaacaatga caacattact taacccctat tttggtgagt
    50761 ttggcggcat gtacgtgcca caaatcctga tgcctgctct gcgccagctg gaagaagctt
    50821 ttgtcagcgc gcaaaaagat cctgaatttc aagcccagtt caacgacctg ctgaaaaact
    50881 atgccgggcg tccaaccgca ctgaccaaat gccagaacat tacagccggg acgaacacca
    50941 cgctgtatct caagcgtgaa gatttgctgc acggcggcgc acataaaacc aaccaggtgc
    51001 tggggcaggc gttgctggcg aagcggatgg gtaaaaccga aatcatcgcc gaaaccgggg
    51061 ccggtcagca tggcgtggcg tcggcccttg ccagcgccct gctcggcctg aaatgccgta
    51121 tttatatggg tgccaaagac gttgaacgcc agtcgcctaa cgtttttcgt atgcgcttaa
    51181 tgggtgcgga agtgatcccg gtacatagcg gttccgccac gctgaaagat gcctgtaatg
    51241 aggcgctacg cgactggtcc ggcagttatg aaaccgcgca ctatatgctg ggcaccgcgg
    51301 ctggcccgca tccttatccg accattgtcc gtgagtttca gcggatgatt ggcgaagaaa
    51361 ccaaagcgca gattctggaa agagaaggtc gcctgccgga tgccgttatc gcctgtgttg
    51421 gcggcggttc gaatgccatc ggcatgtttg ctgatttcat caatgaaacc aacgtcggcc
    51481 tgattggtgt ggagcctggt ggtcacggta tcgaaactgg cgagcacggc gcaccgctaa
    51541 aacatggtcg cgtgggcatt tatttcggta tgaaagcgcc gatgatgcaa accgaagacg
    51601 ggcagattga agaatcttac tccatctccg ccggactgga tttcccgtct gtcggtccac
    51661 aacacgcgta tcttaacagc actggacgcg ctgattacgt gtctattacc gatgatgaag
    51721 cccttgaagc cttcaaaacg ctgtgcctgc acgaagggat catcccggcg ctggaatcct
    51781 cccacgccct ggcccatgcg ctgaaaatga tgcgcgaaaa cccggataaa gagcagctac
    51841 tggtggttaa cctttccggt cgcggcgata aagacatctt caccgttcac gatattttga
    51901 aagcacgagg ggaaatctga tggaacgcta cgaatctctg tttgcccagt tgaaggagcg
    51961 caaagaaggc gcattcgttc ctttcgtcac gctcggtgat ccgggcattg agcagtcatt
    52021 gaaaattatc gatacgctaa ttgaagccgg tgctgacgcg ctggagttag gtatcccctt
    52081 ctccgaccca ctggcggatg gcccgaccat tcaaaacgcc actctgcgcg cctttgcggc
    52141 aggtgtgact ccggcacaat gttttgaaat gctggcactg attcgccaga aacacccgac
    52201 cattcccatt ggcctgttga tgtatgccaa tctggtgttt aacaaaggca ttgatgagtt
    52261 ttatgcccag tgcgagaaag tcggcgtcga ttcggtgctg gttgccgatg tgccagttga
    52321 agagtccgcg cccttccgcc aggccgcgtt gcgccataat gtcgcaccta tcttcatctg
    52381 cccgccaaat gccgatgacg acctgctgcg ccagatagcc tcttacggtc gtggttacac
    52441 atatttgctg tcacgagcgg gcgtgaccgg cgcagaaaac cgcgccgcgt tacccctcaa
    52501 tcatctggtt gcgaagctga aagagtacaa cgctgcacct ccattgcagg ggtttggtat
    52561 ttccgccccg gatcaggtaa aagcagcgat tgatgcagga gctgcgggcg cgatttctgg
    52621 ttcggccatt gttaaaatca tcgagcaaca tattaatgag ccagagaaaa tgctggcggc
    52681 actgaaagtt tttgtacaac cgatgaaagc ggcgacgcgc agttaatccc acagccgcca
    52741 gttccgctgg cggcatttta actttcttta atgaagccgg aaaaatccta aattcattta
    52801 atatttatct ttttaccgtt tcgcttaccc cggtcgaacg tcaacttacg tcatttttcc
    52861 gcccaacagt aatataatca aacaaattaa tcccgcaaca taacaccagt aaaatcaata
    52921 attttctcta agtcacatat tcctcaggta attgttaata tatccagaat gttcctcaaa
    52981 atatatttcc ctctatcttc tcgttgcgct taatttgact aattctcatt agtgactaat
    53041 tttaatgagt gtcgacacac aacactcata ttaatgaaac aatgcaacgc aacgggagaa
    53101 ataacatggc cgaacatcgt ggtagttcag gaaatttcgc cgaagaccgt gagaaggcat
    53161 ccgacgcagg ccgtaaaggc ggtcagcata gcggcggtaa ttttaaaaat gatccgcaac
    53221 gcgcatctga agcgggtaaa aaaggcggtc aacaaagcgg tggtaataaa tcaggcaaat
    53281 cctgattcac cgttaattat taatatcagc tgataaagat aattgcatct gcgggccgta
    53341 tggctcgcag tgatttcact ggagaaaaat atgaatatga agaccattga agatgtattt
    53401 attcaactgc tttcagatac ctacagcgca gaaaaacaat taacccgggc actggcaaaa
    53461 ctcgcaagag caacatcaaa tgaaaaatta agtcaggctt ttcatgcgca cctcgaggaa
    53521 actcatggac agattgaacg aattgatcaa gttgtggaat cagaatcaaa tctgaaaatt
    53581 aagcgcatga aatgtgtggc aatggaaggt cttattgaag aagctaatga ggtcatcgaa
    53641 agtaccgaga aaaacgaagt gcgtgatgcc gcactgattg ccgcagcaca gaaagtcgag
    53701 cattatgaga ttgccagtta cgggacatta gcgacgctgg ctgaacaatt aggttaccgt
    53761 aaagcagcga agcttctgaa agaaaccctg gaagaagaaa aggccaccga catcaaactg
    53821 actgatctgg cccttaataa cgtaaataag aaagccgaaa ataaagcctg aaatatgaat
    53881 tttaactttt agtcatttta taaagaggac attttcatga atcgtattga acattatcat
    53941 gactggttac gtgacgccca cgcaatggaa aagcaagccg aatctatgct tgaatccatg
    54001 gccagccgta tagataatta tcctgaacta cgcgctcgta ttgaacaaca tcttagtgaa
    54061 accaaaaacc agattgttca actggaaact attcttgatc gtaatgacat ttcacgttca
    54121 gtcattaaag attccatgag taaaatggct gcgcttgggc agtcaatcgg tggtatattc
    54181 ccttctgatg aaatagttaa aggctctatt ggcggatatg tcttcgagca atttgaaatc
    54241 acctgttaca cctcactatt agcagcagca aaaaatgccg gtgatacagt ctcaattccg
    54301 atcatcgaag cgattttaaa tgaggaaaag caaatggccg actggctgat tcagcatatt
    54361 ccgcaaacaa ctgagaaatt tttaattcgt tctgaaactg atggcgtaga agcgaagaaa
    54421 taataccctt tataccatgt ccttattgac cccgtatatt acggggtcgt ttttgtgcgg
    54481 aattaaaaac gatatcctgc tgagaacata aacacccacg gatcgaggcg tacgctatcg
    54541 tgttgctgtg caccgcctag cttataattg gcggtggtat cgatatccat gtaccacact
    54601 gacatgttaa ccaaccagtc acggttaatc agataatcaa cccccacctg cccggcagct
    54661 ccccaggaat ctttcagact gagatcggaa agccccgcct ctttgccatg atcgttaaat
    54721 ccattatcaa agaaggtggt gtagttaata ccggccccaa cgtaaggacg gaatttgctg
    54781 ctggcatcac caaaatacca ctgcgccatc agtgttggcg gcagatggtg aacggttgca
    54841 atatcgccgg tcgcccgggt gccgatttta tggcggaacg gcgtcgctgc cagtaattcc
    54901 acaccaatgt tgtcggtcgc catataagta aacgtcaagc ccagttgagt gttattggtc
    54961 acgctgaatc cacccagact tcctaacgta ccaccagcac cttctgttgg acgtacggtt
    55021 gcagaacctg cacgcataaa aaattcgcct gcttcatgcg caaaggcact gccagaaaga
    55081 agagttgtta ctgccaaagc cgccactgtt aactttttca tatccgctcc gtcgttatgg
    55141 ttataaaaac gtgacgaata tacctacatt ggagtaacaa atgatcctac atagatcaca
    55201 ttacatatga taattcacca tttattgatc tggattaatt tagaagttgc attgagaatc
    55261 ctggttaatg tgatttaaat caatttttaa atattttctc tacgcaaata ttaactcgat
    55321 cgccacttgc cgcgctttgc ccacgcctga aggccagcaa ttacctccgc tttacaaaga
    55381 tatgcttttc catacaagac ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc
    55441 cgctaattaa cacctgcata aaactcaagg agagtgcatg tctatcacgg cgcagtccgt
    55501 ataccgtgac accggaaatt tcttccgtaa tcaatttatg accattctgt tggtatcgtt
    55561 gctatgtgcg tttatcacag tggtgttagg gcatgttttc tcacccagtg atgcacagct
    55621 tgcgcagctc aatgacggcg tgcccgttag cggcagtagt gggttgttcg acctggttca
    55681 gaatatgtca ccggaacagc aacaaatttt gctgcaggct tcagcggcgt ccactttttc
    55741 agaattaatc ggtaacgcca ttctcgccgg aggcgtaata ttaattatcc agctggtgtc
    55801 tgcgggtcag agagtcagtg cgctgcgagc tattggtgcc agtgcgccga tattgcccaa
    55861 gttatttatt ctgatttttc tgactaccct tttagtacag attggcatca tgctggtggt
    55921 cgttccggga attatcatgg ccattttact ggccctggca ccggtgatgc tggtacagga
    55981 caaaatgggc atttttgcct cgatgcgtag cagtatgcgg ctgacttggg cgaatatgcg
    56041 tctggtggca cccgcagtac tgagctggtt gctggcaaaa acactgttgc tgctttttgc
    56101 ctcttctttt gccgcattaa ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa
    56161 cctgatttct gccatattgc tcatctatct gttccgcctg tatatgttga ttcgccaata
    56221 accttaagca tttgattacg gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg
    56281 gttgtctttt tcgcgtttta caagatttat gacatctatg cggctactgc agcgctgatc
    56341 gtcgccacgg cgattgtgct tatctatagc tgggttcgct ttcgtaaggt tgagaagatg
    56401 gccctgatca cttttgttct ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat
    56461 gatgagttta ttaaatggaa ggttacagtc atttatgctc tgtttgcggg agccctgtta
    56521 gtcagccaat gggtgatgaa aaagccgcta attcagcgga tgctgagtaa agaactcacg
    56581 ctgccgcaac cggtatggtc gaagctaaat ctggcctggg ctgttttctt tatcctttgc
    56641 ggtctggcaa acatctacat cgcattctgg ctgccgcaaa atatttgggt caactttaaa
    56701 gtctttggcc tgaccgccct taccttaatc ttcacattgt taagcggtat ctatatctac
    56761 cgccacatgc cgcaggaaga taaatcctaa ccagactgcc agtccttcgg ggctggcgct
    56821 gtatctcctc tgttctcctc aggaaaatca tagtagcatc gcgcctgtga ttttcctttt
    56881 aagtcggttt taccatgtct acaacacata acgtccctca gggcgatctt gttttacgta
    56941 ctttagccat gcccgccgat accaatgcca atggtgacat ctttggtggt tggttaatgt
    57001 cacaaatgga tattggcggc gctattctgg caaaagaaat tgcccacggt cgcgtagtga
    57061 ctgtgcgggt tgaaggaatg actttcttac ggccggttgc ggtcggcgat gtggtgtgct
    57121 gctatgcacg ctgtgtccag aaagggacga catcggtcag cattaatatt gaagtgtggg
    57181 tgaaaaaagt agcgtctgaa ccaatcgggc aacgctataa agcgacagaa gcattattta
    57241 agtatgtagc ggttgatcct gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa
    57301 aagcctccgg tcggaggctt ttgactttct gcttactgaa tttcggtggt gccgttaatt
    57361 ttaaacagga tattcaccac aatcccactg cctggcttac ccggctcata acgccatctg
    57421 cgcatcgcat ttttcacctc acgctcaaac atgttcgcag gcttggctga gaggatttgt
    57481 acgttatcca cgcgaccatc cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg
    57541 cgcaatgcct gtgctcgtgc cggatactgc ggctgattac ggcttaatgc gcgtggtcct
    57601 gaagccacac tggtaaccgg cttgctggtt gcagccgttg ccgtacttga tgtggggcgc
    57661 gcgggtgccg tattttcaaa cggtgatgcc ggacgcgact ctacgggttt gacatcgcgt
    57721 tttggctgct cctgtacctt tttcaccggc ttcggttttg gcttaggttt cggcttcggc
    57781 ttttcaatga ccaccggtgc ttcttttggc ggttcgggga tcggctcagg ttccggttct
    57841 ggctctacca ccggctccgg tggcggctga acggcttgtg gcggttcgag atcagcaggc
    57901 gcaaccatcg tgacagaaat tggttgcgca ggcgcaggta gttcaataac ctgatgtacc
    57961 gaggtataga gcagccccgc cacaacagca ccatgaatgc agaccgaaag taacgtcggc
    58021 caggggaagc ggcgaggtaa atcaagggtt attgaagtca taatcatttc agtagaaaaa
    58081 ctaggcctcg attttaaatg caaatagcaa tcatattcaa taaggcaaga cgatcaggat
    58141 cagtgaaaat gtcatttttg gcgtaattac ccttacttta tgcatgacct taacaaagac
    58201 atcatctttc cgttgcagat cttcgccctt tcaaataacg tactttacaa ctttcccgaa
    58261 caaggagttg tgcccgtgtt gtatgttatt tacgctcaag acaaagctga tagcctcgaa
    58321 aaacgccttt ccgttcgtcc ggcacattta gcacgtttac agttactgca tgatgaaggt
    58381 cgtttgttga cagctggtcc aatgccagcg gtagacagta acgatccagg ggctgcggga
    58441 tttactggct caaccgttat tgctgaattt gaatcgctgg aagcagctca ggcctgggcc
    58501 gatgctgacc cgtacgtagc ggcaggagtc tatgaacacg tttcagtgaa accatttaag
    58561 aaagtgttct gag
//
LOCUS       DAJTUI010000015        57876 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTUI010000015 DAJTUI010000000
VERSION     DAJTUI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.015"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..124)
                     /locus_tag="OO464_000996"
     CDS             complement(<1..124)
                     /locus_tag="OO464_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR6226234.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQR"
     gene            279..851
                     /gene="yeiP"
                     /locus_tag="OO464_000997"
     CDS             279..851
                     /gene="yeiP"
                     /locus_tag="OO464_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6226235.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1074..2540
                     /locus_tag="OO464_000998"
     CDS             1074..2540
                     /locus_tag="OO464_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6226236.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2658..3644
                     /gene="yeiR"
                     /locus_tag="OO464_000999"
     CDS             2658..3644
                     /gene="yeiR"
                     /locus_tag="OO464_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6226237.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3683..4396
                     /gene="lpxT"
                     /locus_tag="OO464_001000"
     CDS             3683..4396
                     /gene="lpxT"
                     /locus_tag="OO464_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6226238.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4808..5374
                     /gene="mepS"
                     /locus_tag="OO464_001001"
     CDS             4808..5374
                     /gene="mepS"
                     /locus_tag="OO464_001001"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6226239.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5555..7111
                     /locus_tag="OO464_001002"
     CDS             5555..7111
                     /locus_tag="OO464_001002"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6226240.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7193..9007
                     /locus_tag="OO464_001003"
     CDS             7193..9007
                     /locus_tag="OO464_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6226241.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9008..10102
                     /locus_tag="OO464_001004"
     CDS             9008..10102
                     /locus_tag="OO464_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6226242.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10102..11127
                     /locus_tag="OO464_001005"
     CDS             10102..11127
                     /locus_tag="OO464_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6226243.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11129..12718
                     /gene="yejF"
                     /locus_tag="OO464_001006"
     CDS             11129..12718
                     /gene="yejF"
                     /locus_tag="OO464_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6226244.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12722..13066)
                     /locus_tag="OO464_001007"
     CDS             complement(12722..13066)
                     /locus_tag="OO464_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226245.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13399..14589)
                     /gene="bcr"
                     /locus_tag="OO464_001008"
     CDS             complement(13399..14589)
                     /gene="bcr"
                     /locus_tag="OO464_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR6226246.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14617..15312)
                     /gene="rsuA"
                     /locus_tag="OO464_001009"
     CDS             complement(14617..15312)
                     /gene="rsuA"
                     /locus_tag="OO464_001009"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6226247.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15462..17222
                     /locus_tag="OO464_001010"
     CDS             15462..17222
                     /locus_tag="OO464_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6226248.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17347..17631
                     /gene="rplY"
                     /locus_tag="OO464_001011"
     CDS             17347..17631
                     /gene="rplY"
                     /locus_tag="OO464_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6226249.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17770..18777)
                     /gene="yejK"
                     /locus_tag="OO464_001012"
     CDS             complement(17770..18777)
                     /gene="yejK"
                     /locus_tag="OO464_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6226250.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            18959..19186
                     /locus_tag="OO464_001013"
     CDS             18959..19186
                     /locus_tag="OO464_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6226251.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19206..20966
                     /gene="pbgA"
                     /locus_tag="OO464_001014"
     CDS             19206..20966
                     /gene="pbgA"
                     /locus_tag="OO464_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6226252.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21041..21117
                     /locus_tag="OO464_001015"
     tRNA            21041..21117
                     /locus_tag="OO464_001015"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21075..21077,aa:Pro,seq:ggg)
     gene            complement(21220..23811)
                     /gene="yejO"
                     /locus_tag="OO464_001016"
     CDS             complement(21220..23811)
                     /gene="yejO"
                     /locus_tag="OO464_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR6226253.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24131..24778
                     /gene="narP"
                     /locus_tag="OO464_001017"
     CDS             24131..24778
                     /gene="narP"
                     /locus_tag="OO464_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR6226254.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(24989..26041)
                     /gene="ccmH"
                     /locus_tag="OO464_001018"
     CDS             complement(24989..26041)
                     /gene="ccmH"
                     /locus_tag="OO464_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6226255.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26038..26595)
                     /gene="dsbE"
                     /locus_tag="OO464_001019"
     CDS             complement(26038..26595)
                     /gene="dsbE"
                     /locus_tag="OO464_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6226256.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26592..28535)
                     /gene="ccmF"
                     /locus_tag="OO464_001020"
     CDS             complement(26592..28535)
                     /gene="ccmF"
                     /locus_tag="OO464_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6226257.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28532..29011)
                     /gene="ccmE"
                     /locus_tag="OO464_001021"
     CDS             complement(28532..29011)
                     /gene="ccmE"
                     /locus_tag="OO464_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6226258.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29008..29217)
                     /gene="ccmD"
                     /locus_tag="OO464_001022"
     CDS             complement(29008..29217)
                     /gene="ccmD"
                     /locus_tag="OO464_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6226259.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29214..29951)
                     /gene="ccmC"
                     /locus_tag="OO464_001023"
     CDS             complement(29214..29951)
                     /gene="ccmC"
                     /locus_tag="OO464_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6226260.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(29993..30655)
                     /gene="ccmB"
                     /locus_tag="OO464_001024"
     CDS             complement(29993..30655)
                     /gene="ccmB"
                     /locus_tag="OO464_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6226261.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30652..31269)
                     /gene="ccmA"
                     /locus_tag="OO464_001025"
     CDS             complement(30652..31269)
                     /gene="ccmA"
                     /locus_tag="OO464_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6226262.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31288..31890)
                     /gene="napC"
                     /locus_tag="OO464_001026"
     CDS             complement(31288..31890)
                     /gene="napC"
                     /locus_tag="OO464_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6226263.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(31900..32349)
                     /gene="napB"
                     /locus_tag="OO464_001027"
     CDS             complement(31900..32349)
                     /gene="napB"
                     /locus_tag="OO464_001027"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6226264.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32346..33209)
                     /gene="napH"
                     /locus_tag="OO464_001028"
     CDS             complement(32346..33209)
                     /gene="napH"
                     /locus_tag="OO464_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6226265.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33196..33891)
                     /gene="napG"
                     /locus_tag="OO464_001029"
     CDS             complement(33196..33891)
                     /gene="napG"
                     /locus_tag="OO464_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6226266.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(33898..36384)
                     /gene="napA"
                     /locus_tag="OO464_001030"
     CDS             complement(33898..36384)
                     /gene="napA"
                     /locus_tag="OO464_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6226267.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36381..36644)
                     /gene="napD"
                     /locus_tag="OO464_001031"
     CDS             complement(36381..36644)
                     /gene="napD"
                     /locus_tag="OO464_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6226268.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36634..37128)
                     /gene="napF"
                     /locus_tag="OO464_001032"
     CDS             complement(36634..37128)
                     /gene="napF"
                     /locus_tag="OO464_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6226269.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37237..37401
                     /locus_tag="OO464_001033"
     CDS             37237..37401
                     /locus_tag="OO464_001033"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226270.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37536..38024
                     /gene="eco"
                     /locus_tag="OO464_001034"
     CDS             37536..38024
                     /gene="eco"
                     /locus_tag="OO464_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6226271.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38146..39177)
                     /locus_tag="OO464_001035"
     CDS             complement(38146..39177)
                     /locus_tag="OO464_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR6226272.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39411..39770)
                     /locus_tag="OO464_001036"
                     /pseudo
     CDS             complement(39411..39770)
                     /locus_tag="OO464_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(39988..41631)
                     /gene="yojI"
                     /locus_tag="OO464_001037"
     CDS             complement(39988..41631)
                     /gene="yojI"
                     /locus_tag="OO464_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6226273.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41707..42357)
                     /gene="alkB"
                     /locus_tag="OO464_001038"
     CDS             complement(41707..42357)
                     /gene="alkB"
                     /locus_tag="OO464_001038"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR6226274.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42357..43421)
                     /gene="ada"
                     /locus_tag="OO464_001039"
     CDS             complement(42357..43421)
                     /gene="ada"
                     /locus_tag="OO464_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6226275.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43495..44550)
                     /gene="apbE"
                     /locus_tag="OO464_001040"
     CDS             complement(43495..44550)
                     /gene="apbE"
                     /locus_tag="OO464_001040"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6226276.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44662..45783)
                     /gene="ompC"
                     /locus_tag="OO464_001041"
     CDS             complement(44662..45783)
                     /gene="ompC"
                     /locus_tag="OO464_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6226277.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46521..49193
                     /gene="rcsD"
                     /locus_tag="OO464_001042"
     CDS             46521..49193
                     /gene="rcsD"
                     /locus_tag="OO464_001042"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6226278.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49210..49860
                     /gene="rcsB"
                     /locus_tag="OO464_001043"
     CDS             49210..49860
                     /gene="rcsB"
                     /locus_tag="OO464_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6226279.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50060..52909)
                     /gene="rcsC"
                     /locus_tag="OO464_001044"
     CDS             complement(50060..52909)
                     /gene="rcsC"
                     /locus_tag="OO464_001044"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6226280.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53184..53960)
                     /locus_tag="OO464_001045"
     CDS             complement(53184..53960)
                     /locus_tag="OO464_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR6226281.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(53965..55614)
                     /locus_tag="OO464_001046"
     CDS             complement(53965..55614)
                     /locus_tag="OO464_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6226282.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55615..56174)
                     /locus_tag="OO464_001047"
                     /pseudo
     CDS             complement(55615..56174)
                     /locus_tag="OO464_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56333..>57876)
                     /locus_tag="OO464_001048"
     CDS             complement(56333..>57876)
                     /locus_tag="OO464_001048"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6226283.1"
                     /translation="TSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNN
                     VRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQ
                     GKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKP
                     AAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQN
                     GQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRN
                     NLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGP
                     GARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLL
                     HRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPL
                     ADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTA
                     AILMV"
BASE COUNT        14162 a        15619 c        14214 g        13881 t
ORIGIN      
        1 agcgttgctg ttcatcaagc tgaatgcagg gcgtattggc gggcttgcca tctggcatac
       61 cgggaatagg gctggaaatt gaaggggcag tgcaacacgc cccgcaaccc ggacggcatt
      121 ccatacgttg ttctcttaag gttaaccacc ggattgatga gcgcgcacag taccaccttt
      181 tttgcaccag caaaagtgcg aataccactt gcccgaaagg cccgtcgcga gtactttgtc
      241 gcgatatttt tgacattttc gactacagga atttttcgat gccaagagcg aacgaaatta
      301 aaaaaggtat ggtactgaat tacaacggca aactgctgct ggctaaggat attgatattc
      361 agtcgcccac tgcccgcggt gccgctacgc tgtacaaaat gcgtttttct gatgtccgta
      421 ccgggctgaa agtagaagag cgtttcaaag gtgatgatat cgttgacacc gtgacgctga
      481 cccgccgtta cgttgacttc tcctatgtcg atggcaacga atatgtcttt atggataaag
      541 aagactatac cccgtatacc ttcaccaaag atcagattga agaagagttg ctgtttatgc
      601 cagagggcgg catgccggac atgcaggtgc tgacctggga cggtcaactg ctggcgcttg
      661 agctgccgca gaccgttgat ctggaaatcg ttgaaaccgc accaggcatc aaaggggcat
      721 ctgccagcgc ccgtaacaaa ccggcgacat tgagcactgg tctggtgatt caggtaccag
      781 aatacttaag cccgggcgaa aaaattcgta tccatatcga agaacgccgt tatatggggc
      841 gtgctgacta acttcagccg catgcagaaa agggatagct caggctgtcc cttttttaat
      901 ttattatacc aatcttctat tttgcgcttc ctgaacacct tacgccctca attttcactc
      961 gttgattgat cgccctcaca cttcatcgca ttaacaatcc agaccaattt caattgctgt
     1021 catataactt tacactgtca ttgttaattt atcgttacta agacgtgact cctatgaaga
     1081 caattgcctc cgttacgctc ccgcatcatg tacacgctcc acgctacgat cgccagcagt
     1141 tgcaatcacg tatcgttcat tttggctttg gcgcctttca ccgcgctcat caggcgttac
     1201 tgaccgatcg tgtgctgaat gcccagggcg gcgactgggg gatctgtgaa atcagcttgt
     1261 tcagcggtga tcaactgatg agccagctcc gcgcacagaa ccatttatat accgtgctgg
     1321 agaaaggtgc ggacggcaat caggtgataa ttgtcggtgc cgttcacgaa tgccttaatg
     1381 caaaactgga ttccttagcg gcaattattg agaaattttg cgagccacag gtggcaattg
     1441 tttccctgac gattaccgaa aaaggctatt gtattgaccc ggccaccggt gcactcgaca
     1501 ccagtaatcc gcggattatt cacgatctac aaacccctga agaacctcac tccgcaccgg
     1561 gtattctcgt cgaagcactg aaacgccgcc gtgagcgcag ccttacaccg tttaccgtgc
     1621 tctcctgcga caatattccc gacaatggcc atgtggtgaa aaacgcggtg ctgagaatgg
     1681 cagaaaaacg ttcgccagaa ctcgccgggt ggataaaaga acacgtcagt tttccgggaa
     1741 ccatggtcga ccgcattgtt ccggctgcaa ccgacgaatc actggcagaa atcagccagc
     1801 atctgggggt aaatgatccc tgcgcgatta gctgcgaacc gtttatccag tgggtggttg
     1861 aagataactt catcgctggg cgtcctgcct gggaagtcgc aggtgtacaa atggtgaatg
     1921 atgtcctgcc atgggaagag atgaaactgc gtatgcttaa tggcagccac tcttttctcg
     1981 cttatctggg ttacctctca ggattcgcct atatcagtga ttgcatgcag gatcgcgtat
     2041 ttcgctatgc tgccagaaca ttaatgctgg atgagcaagc gccgacactg caaattaaag
     2101 atgtcgattt aacacaatat gcggataagt taattgcacg ttttgctaat ccgtcgctga
     2161 aacataagac ctggcaaatc gcgatggatg gcagccagaa attaccgcaa cgtatgctgg
     2221 caggtattcg catacatctg gggcgtgaaa cggactggtc gttgctggca ttaggcgttg
     2281 ctggctggat gcgttacgtc agcggcgttg atgatgccgg aaatgccatt gatgttcgcg
     2341 atccgcttag cgataaaatt cgcgaacttg ttgcgggcag cagcagtgaa caacgcgtaa
     2401 ccgccctgct ttccctgcgt gaagttttcg gtgatgatct gccagataac ccgcattttg
     2461 tgcaggccat cgaacaagcc tggcaacaaa tcgtacaatt cggcgcacat caggcgctat
     2521 taaacaccct caaaatttaa cgatttctgc ggttaaagcg gatgaagctc accttcgtcc
     2581 gctctcccct tctcttttct gcctttttta gccaggatta acgctcagtt aacttaccag
     2641 agtattgcgg agccgttgtg accaggacca acctcatcac cggttttctc ggcagcggga
     2701 aaaccacgtc gattcttcat ctgttagccc ataaagatcc caacgaaaaa tgggcggtac
     2761 tggttaatga atttggggaa gtcggaattg atggtgcttt gctcgccgat agcggcgcat
     2821 tgctgaaaga gatccccggc ggctgcatgt gctgcgttaa tggtttaccc atgcaggtag
     2881 ggttgaatac cttactgcgt cagggaaaac cagaccgctt gttgatagag ccgaccgggc
     2941 tgggccatcc gaaacagatc ctcgatctgt taaccgcacc agtctatgaa ccgtggatag
     3001 atctgcgcgc caccttgtgc attctcgatc cacgcctgct gctggacgaa aaaagcgcca
     3061 gcaatgaaaa cttccgtgac cagctggctg ccgcagacat cattgtcgcc aataaatccg
     3121 accgtgcgac gcccgaaagt gagcaagcgc tacagcgttg gtggcagcaa aatggtggcg
     3181 atcgacaatt aattcacagt gagcatggga aagttgacgg tcatcttctg gatttgccgc
     3241 gtcgcaattt agccgagttg cccgccagcg ccgcgcattc tcatcagcat gtcgtgaaaa
     3301 aagggttagc agcgttaagc ctgccagagc atcaacgctg gcgtcgcagt ctgaacagcg
     3361 ggcaaggata tcaggcctgc ggctggatat tcgacgctga tacggtattc gacaccattg
     3421 gcattctgga atgggcgcga cttgcaccgg tggaacgcgt caaaggcgtg ctgcgtattc
     3481 ccgaagggct ggtacgaatc aaccgtcagg gcgatgacct gcacattgaa acgcaaaacg
     3541 ttgcgccacc ggacagccgt attgagctga tttccagcag cgaagctgac tggaatgcct
     3601 tgcagagcgc gctgttgaag cttcgtttag cgactaccgc gtaaggttgc ctgcgttttt
     3661 cagtaagata attagagaaa atatgattaa aaatttgccg caaatagtgt tgttgaatat
     3721 tgtcggcccc gcgctgtttc tttcctggta tattcccgtt aatcatggat tctggttgcc
     3781 gattgatgcg gatatttttt atttctttaa tcagaaactg gtcgaaagta aggccttttt
     3841 atggctggtt gcattgacta acaatcgcgc cttcgacggt tgttcactgc tggcgatggg
     3901 tatgttgatg ctgagtttct ggctgaaaga aaacgcccct ggcagacgac gtatcgtgat
     3961 tatgggtctg gtcatgctat taactgcagt ggtattaaac cagctgggtc aggcattaat
     4021 tcctgtaaaa cgggccagcc caacattgac ttttaccgat attaaccgcg tcagcgaact
     4081 gctctctgtt cccacgaaag atgcctcacg agatagcttt cccggcgatc acggcatgat
     4141 gctgcttatt ttttcggcat tcatgtggcg ttatttcggc aaagttgcag gccttatcgc
     4201 ccttattatt tttatggttt ttgcatttcc cagagtaatg attggcgcac actggtttac
     4261 tgacatcatt gtcggttcga tgaccgtgat attgatcggt ttgccctggg tgttgctgac
     4321 gccattaagt gatcgattaa tcaccttttt tgacaaatca ctaccaggaa aaaacaaaca
     4381 tttccaaaac aaataactca cagtaattaa catcatcagg gttattttta tagtgaggat
     4441 aatcctgatg atgcgcaccg tgctttcatc tatcgaacgc aaaaatcatt ctctaagtaa
     4501 atgaatggat tgcatgcgtt tcactcaatt gtactttaat tgaccaaccc cgcttattaa
     4561 ctttctgtat cactttttct tataaaaaat catgtaaaac cgctcgccaa gaccgcacca
     4621 atcgggtaat ctcgaactcg ttttgcctcg gcggtagatt atcctcacag catataattt
     4681 tgtgcgttag tccacagatt tggccttaag gaattgtttc aacatgccca ggtaattagt
     4741 ctcgtgtcgc ttggcatttt tttataacga tatttgtcgt taaggacttc aagggaaaac
     4801 aaacaacatg gtcaaatctc aaccgatttt gagatatatc ttgcgcggga ttcccgcgat
     4861 tgcagtagcg gttctgcttt ctgcatgtag tgcaaataac accgcaaaga atatgcatcc
     4921 tgagacacgt gcagtgggta gtgaaacatc atcactgcaa gcttctcagg atgaatttga
     4981 aaacctggtt cgtaatgtcg acgtaaaatc gcgaattatg gatcagtatg ctgactggaa
     5041 aggcgtacgt tatcgtctgg gcggcagcac taaaaaaggt atcgattgtt ctggtttcgt
     5101 acagcgtaca ttccgtgagc aatttggctt agaacttccg cgttcgactt acgaacagca
     5161 ggaaatgggt aaatctgttt cccgcagtaa tttgcgtacg ggtgatttag ttctgttccg
     5221 tgccggttca acgggacgcc atgtcggtat ttatatcggc aacaatcagt ttgtccatgc
     5281 ttccaccagc agtggtgtta ttatttccag catgaatgaa ccgtactgga agaagcgtta
     5341 caacgaagca cgccgggttc tcagccgcag ctaataaacc gtttggatgc aatcccttgg
     5401 ctatcctgac gagttaactg aaagcactgc ttaggcagtg cttttttgtt ttcattcatc
     5461 agagaaaatg atgtttccgc gtcttgatcc aggctatagt ccggtcattg ttatctttta
     5521 aatgttgtcg taatttcagg aaattaacgg aatcatgttc atacgcgctc ccaattctgg
     5581 acgtaagctc ctgcttacct gcattgtagc aggcgtgatg attgcgatac tggttagctg
     5641 ccttcagttt ttagtggcct ggcataagca cgaagtcaaa tacgacacac tgattatcga
     5701 cgtacaaaag tatctcgata cctattttgc cgacctgaaa tccactactg accggcttca
     5761 gccgctgacc ttagatacct gccagcaggc taaccccgaa ctgaccgctc gcgcggcgtt
     5821 tagcatgaat gtccgtacgt ttgtgctggt gaaagataaa aaaacattct gttcatctgc
     5881 gactggtgag atggacattc cactcaatga attgattccg gcgctcgaca ttaataaaaa
     5941 cgtcgatatg gcgatcttac ccggcacgcc gatggtgccg aacaaacccg caatcgtcat
     6001 ctggtatcgc aaccctttgc tgaaaaatag cggtgtgttt gccgctctga atctcaacct
     6061 gacgccttct ctcttttata gttcacggca ggaagattac gatggcgtcg ccctcattat
     6121 tggcaatact gcgctatcta ccttttcttc acgtttgatg aacgttaatg aattaaccga
     6181 catgccggtc catgaaacta aaattgcggg cattcctctg accgttcggc tttatgcgga
     6241 tgactggaca tggaacgatg tgtggtacgc atttttactg ggcggcatga gtggaactgt
     6301 cgttggcctg ctttgctatt acctgatgag cgtacgtatg cgccccgaca gagaaatcat
     6361 gaccgccatc aagcgcgaac aattttacgt ggcgtatcaa ccggtggtgg atacacaagc
     6421 tttgcgggta acgggcctgg aagtactgct acgctggcgg catcctgtag cgggagaaat
     6481 tcccccggat gccttcatta actttgccga atcgcaaaag atgattgtgc cgctgactca
     6541 gcacctgttt gagttaattg cccgcgatgc cgcagaatta gaaaaagtac tgccggtagg
     6601 cgtcaaattt ggcattaaca ttgcgccgga ccatctgcac agcgaaagct ttaaagcaga
     6661 tatccagaaa ctgctcactt ccctgcccgc acaccatttc cagattgtgc tggaaattac
     6721 cgagcgcgat atgctgaaag agcaagaagc cacacaactc ttcgcctggc tgcactcggt
     6781 cggcgtagaa attgctattg atgacttcgg caccgggcac agcgcgctta tctatcttga
     6841 gcgttttacg ctcgattatc tgaaaattga ccgtggattt atcaacgcca tcggtacgga
     6901 aacgatcact tcacccgtac ttgacgcggt gctgacgctg gcgaaacgtc tcaatatgct
     6961 gacagttact gaaggggtcg aaacgccgga acaagcgcga tggctaagcg aacgcggcgt
     7021 taatttcatg caaggctact ggattagtca cccgttaccg ctggacgatt ttgttcgctg
     7081 gctgaagaaa ccgtatacgc cgcagtggta aggtgtgctt acgtccctta ttattcatag
     7141 tgaaagcatg ccggattgcg gctaatgatg agtaaaagga aatccgttgc agatgattgt
     7201 gcgcatactg ctgctgttta tcgctctgtt caccttcggt gcgcaggcgc aggctatcaa
     7261 ggaaagctat acctttgccg tgctgggcga accccggtac gcgtttaatt tcaaccattt
     7321 tgattatgtg aaccccgccg cgccaaaagg tgggcagata acgttgtcag ccctcggtac
     7381 cttcgataat ttcaaccgct atgcactgcg cggcaacccg ggcgcacgca ccgagcagct
     7441 gtacgacacg ttatttacga cttccgatga cgaaccaggc agttattacc cgctgattgc
     7501 tgaaagtgca cgctatgctg acgattattc ctgggtggag gtcgctatta atccgcgcgc
     7561 ccgttttcat gatggttctc ccattactgc ccgcgatgta gagtttactt ttcaaaaatt
     7621 tctgaccgaa ggcgtgccgc aatttcgtct ggtctacaaa ggcaccaccg tcaaagccat
     7681 tgcgccgtta accgtgcgca ttgagttagc taaacccggc aaagaagata tgttgagtct
     7741 gttttcgctg ccggtatttc cagaaaagta ctggaaggat cacaaactta gcgacccgct
     7801 cgccacgcct ccgcttgcca gtggtccgta ccacgttacg tcctggaaaa tggggcaaaa
     7861 tattgtctat tcccgcgtga aagattactg ggcagcaaac ttaccggtaa accgtggacg
     7921 ctggaatttc gacaccattc gctacgatta ttacctcgat gataatgtcg cctttgaagc
     7981 gtttaaagca ggtgcctttg atttgcgtat ggaaaacgac gctaaaaact gggccacgcg
     8041 ttataccggt aaaaatttcg ataaaaaata catcatcaaa gatgagcaaa agaacgaatc
     8101 agcccaggat acgcgctggc tggcgtttaa tatccaacgt gcggtattca gcgatcgccg
     8161 ggtgcgggaa gcaatcactc tcgcctttga ctttgaatgg atgaacaagg cgttgtttta
     8221 caacgcctgg agtcgcacga acagttattt tcaaaatacc gaatacgcgg ccagaaatta
     8281 ccccgacgcc gcggagctgg tgcttctggc accaatgaaa aaagatctac cgccagaagt
     8341 cttcacacaa atctaccagc cgccggtatc taaaggcgat ggctacgatc gtgacaacct
     8401 gttaaaagcc gacaaacttc tcaacgaagc gggctgggtg ctgaagggtc agcaacgcgt
     8461 taatgccaca acgggtcagc cgctcagctt tgaattattg cttcccgcaa gcagcaatag
     8521 tcagtgggta ttgccgttcc agcacagcct gcaacggctg ggtatcaata tggacattcg
     8581 caaggtggat aactcgcaaa tcactaaccg catgcgcaat cgcgactatg acatgatgcc
     8641 gcgactatgg cgggcgatgc cgtggcccag ttccgattta cagatttcct ggtcatcgga
     8701 atatatcaat tccacttata atgcccccgg cgtgcaaagc ccggttatcg actcactgat
     8761 caaccaaatt attgccgcgc agggaaataa agaaaaatta ctgccgttgg ggcgagcact
     8821 ggatcgcgta ttaacgtgga attattacat gctgccaatg tggtacatgg cggaagaccg
     8881 tctcgcctgg tgggataaat tctcccaacc cgctgtacgc cctgtttaca gcctgggtat
     8941 cgatacctgg tggtatgacg ttaataaagc ggccaaactg ccgtcagcca gacaacaggg
     9001 agagtagatg ggcgcttacc tgattcgccg tctgttgctg gtgatcccaa cgttatgggc
     9061 tattatcact atcaactttt tcatcgtgca aattgcgcct ggcggtccgg tcgatcaggc
     9121 catcgccgcc attgagtttg gtaatgccgg agtattaccc ggcgcaggcg gtgaaggtgt
     9181 tcgtgccagc catgcgcaaa cgggtgtcgg caatatcagc gacagtaatt accgtggcgg
     9241 acgcggatta gatccagaag tgatcgctga gatcactcat cgctacggtt tcgataagcc
     9301 gatccacgaa cgttacttca aaatgctctg ggactacatc cgctttgatt ttggtgatag
     9361 cctgtttcgc agcgcctcgg tgctgacgct gattaaagac agtctgccgg tttccatcac
     9421 cctcggattg tggagcacgc tgattatcta tctggtgtcg attccgttag gcattcgcaa
     9481 agctgtttat aatgggagtc gctttgacgt ctggagtagc gcatttatca tcatcggcta
     9541 cgccattccg gcctttttgt ttgccatcct gctgattgtc ttcttcgcgg gcggcagcta
     9601 tttcgacctg ttccctctac gcggcctggt ttccgctaac tttgattcgc tgccgtggta
     9661 tcagaaaatc accgattatc tgtggcatat cacgctaccg gtgctggcga cagtgattgg
     9721 tggctttgcg gcgctgacca tgctgaccaa aaactcattc cttgatgaag tgcgcaagca
     9781 atacgtggtg accgcgcgtg cgaaaggggt aagtgagaaa aacattctct ggaaacatat
     9841 gttccgcaac gccatgctgc tggtgattgc cggttttccg gcgacgttta tcagcatgtt
     9901 ttttaccggc tcgctgctga ttgaggtgat gttttcactc aatggtcttg gcttactggg
     9961 ctacgaagcg accgtctcgc gcgattatcc tgtaatgttt ggtaccttgt atattttcac
    10021 cctgattggc ctgctgctga atattgtcag tgatatcagc tatacgctgg ttgatccgcg
    10081 tatagatttt gagggacgtt aatgtcgcga ctcagccccg tcaatcaggc ccgttgggcg
    10141 cgttttcgtc ataaccgtcg cggctactgg tcgttatgga ttttcctcgt tttgtttggt
    10201 ttgagtttgt gttctgaact tatcgctaac gataaaccgt tgctggtgcg ttatgacggc
    10261 agttggtatt tcccgttgtt gaaaaactac agcgaaagcg attttggcgg cccgctggca
    10321 agtcaggctg attatcagga cccgtggctg aaacaacggc tggaaaataa cggctgggta
    10381 ctgtgggcac cgattcgctt tggtgctacc agtatcaact ttgctaccga taagcccttc
    10441 ccttctccac cctcccggca aaactggctg ggaacggatg caaacggcgg cgatgtgctg
    10501 gcacgcattc tctatggcac gcggatctcg gttctgtttg gcctgatgct gactctctgt
    10561 tccagcgtga tgggcgtgct ggcgggggcg ctacaaggct attacggcgg taaagtcgat
    10621 ctctgggggc aacgctttat tgaagtatgg tcggggatgc cgacgctgtt tttgattatt
    10681 ttactttcca gcgtcgtaca gcctaacttc tggtggctgc tggcaattac tgtcttgttt
    10741 ggctggatga gtctggtcgg cgtggtgcgg gcggagtttt tacgtactcg taatttcgac
    10801 tacattcgtg cggcacaggc gcttggcgtc agcgatcgca gtatcatcct gcgtcatatg
    10861 ttgcctaatg ccatggtcgc gaccctcacc tttttaccgt ttattttatg tagttcgata
    10921 accaccctga cctcgctcga tttcctcggc ttcggtctac cgctcggttc accgtcactc
    10981 ggtgaactgc tgttacaagg gaaaaataac cttcaggccc cgtggcttgg gatcaccgcc
    11041 ttcttgtcgg tggcgatatt attgtctttg ctgatcttta ttggtgaagc cgtccgcgac
    11101 gcatttgatc ctaataaggc ggtgtagcat gacgcaaact ctgttagcga ttgaaaattt
    11161 gtcggtgggt tttcgccatc agcaaaccgt acgtacagta gtcaatgatg tttcactaca
    11221 gattgaggct ggcgaaacgc tggcgctggt gggtgagtca ggttcaggca aaagcgttac
    11281 cgcgctgtca attttacgcc tgctcccttc cccgccggtt gaatatctct ccggcgatat
    11341 tcgttttcat ggcgaatcgc tgcttcatgc cagcgatcaa acgttgcgcg gtgtacgcgg
    11401 taataagatc gccatgattt ttcaggaacc gatggtgtcg ttaaatccat tgcataccct
    11461 ggaaaaacag ctttatgaag tgctttcact ccaccgcggg atgcgtcggg aagcggctcg
    11521 tggcgaaatt cttaactgcc ttgatcgcgt cggtatccgc caggcggcaa aacggctgac
    11581 agattatccg catcagctct ccggcggcga acgccagcgg gtgatgattg cgatggcgct
    11641 gttaacgcga ccggaattat taattgccga tgaaccgacc accgcactgg acgtctctgt
    11701 ccaggcgcag attttacagc tgttgcgcga actgcaaggc gagctgaata tgggcatgct
    11761 gtttattact cataacctca gcattgtcag aaaactggcc caccgcgtgg cggtaatgca
    11821 aaacggtcgc tgtgtcgagc aaaataacgc cgctacgcta tttgcctcac ccactcatcc
    11881 ttacacacaa aagctactca acagtgaacc gtcaggcgat ccggtaccgt tgccagaacc
    11941 tgcctcaacg ttgctggatg ttgaacagct tcaggttgcc ttccccattc gcaaagggat
    12001 tttgaagcgc attgtggatc ataatgtggt ggtgaaaaac atcagtttta cgctacgggc
    12061 gggtgaaaca ctgggtttag tgggcgagtc tggttccggg aaaagcacga cgggactggc
    12121 gctgctgcga ctgattacct ctcagggcag catcgtcttt gacggtcagc cactgcaaga
    12181 tttaaatcgc cgccagctgt tacctactcg tcatcgtatt caggtggtat ttcaggatcc
    12241 aaactcctcg ctcaacccac gactcaacgt tttgcagatt attgaggaag gcttacgggt
    12301 tcaccagccg acgctttctg ccgcacaacg cgaacaacaa gtgatagccg tgatgcatga
    12361 agtgggatta gatcctgaaa cacgccaccg ttatccggcg gagttctctg gtggtcagcg
    12421 acaacgtatt gcgattgcca gggcattaat tcttaagccc tcgctgatca tacttgatga
    12481 accgacatca tcactcgaca aaacggtaca ggcgcaaata ttgacgcttt tgaaatcatt
    12541 gcaacaaaag catcaactgg cctatttgtt tatcagccac gatttgcacg ttgtccgcgc
    12601 gttatgtcat caggttatcg tactgcgaca aggggaagta gtggaacaag gaccgtgcgc
    12661 gcgcgtgttt gccacaccgc agcaggagta tacgcgtcag ttactggcgt tgagctgacg
    12721 cttaaaaagg attgtagttt gaaaagggtt cggcaattgc cacaccaaaa tttttcaatc
    12781 ggcatgttgc agcaaactcg tcctggcgat ttacaaacag gcacggctca ccttcacact
    12841 ccaacaccga acatggaact tcgatgtcgc ttaacgcctg gcttagttta tacatcaccg
    12901 accatgcatt atcaccatcc gggctaagca atttaagcgc caccaggctg ttgtcaccgc
    12961 acggtccatt ttgaggaatc ggttcaacct tcgaacctgc gaaaccagca gaccagcgat
    13021 agttctgcgg cagtcgatgg acgattgaga gttgtaatga agtcacttat tttccccgga
    13081 agtacattca cttcacaatt tgtttacatc aattttaaca catcatcaac aatccgtctt
    13141 tatacagatc gtaaagattg atgcctcgcc tgcgtggcct ctatggctct gatttaagta
    13201 atatctgcgg gcgtggtcac gtttgcgggg ctatatgtac ccgtttctgc gatctaactc
    13261 aaccttttta actacaatga tgtgactttt tacataaatt gattttacat aaaataaaca
    13321 tatatcgggg aaatgataga tttgtggttg acatgcatca acaaaggaag ccttttacct
    13381 tcctcgttgt gcaatagatc accgtttttt cggccgactg gcgtacagac agaagagaat
    13441 ggagctggtt gcgcagaatg caattgacca aatcatcggc catgcagagt taaaggtcgc
    13501 aagagaaagc aatgcgccaa caattgcccc tatgccaaaa cggaaggttc ctgccagcga
    13561 agatgccgtt cccgccatat ggggaaactc atcaagaatg accgccatcg cattggatga
    13621 caccatcgac acgcagccca caaacgccgc aacgccaacc accagcgacc agaatcccag
    13681 ccccagcggc gcactgatga ccatccacgc agccataata aattgtatcc acaaccccga
    13741 gcggaacata tttaacgcgc caatgcggcg gacgaagcgg ctgttaaaga tggtcatcac
    13801 gaacagaaaa acaatgttta gcgcaaagta ataaccaaag ttttccggcg cgacgtggtt
    13861 aatttcaata taaacaaacg gtccggcgct taagaatgag aacatcccgg caaagctgaa
    13921 accactggca agcatgtagc tcaggacacg tttatggcgg aacagcgccg caaagttacc
    13981 aatagtggta cgaatgtgaa atggctgacg acgctccggt ggtaaggttt ctttaatcag
    14041 gaagaaaatc attgccgaag ccagaatcgc cgctaatgcc aggatccaga agatgtaatg
    14101 ccagctcagc cacaccagca cccagccgcc aactatcggt gccatcagca gtgcaatggt
    14161 cgtcaccagc atgacaaacg acatcatccg cgagaactct tctttcgggt aaatatcacg
    14221 catcaaagcg ttgataacca cgctggccgc agccgcagcc agcccgtgga agaaacgcat
    14281 cacaatcagc tgatcgatgg tttgtgccaa cgcacacgcc accgcggcgg cggcaaacac
    14341 cagcgtcccg ccaagaacca ccggcttacg cccgaagctg tctgccatcg gcccgtagat
    14401 taactgcccc aacgcaaagc ccagaatata agtactgagg gtcatctgcg tactgcccgc
    14461 cgatacgcca aactgcgctg aaattaccgg tagcgcgggc agatacatat caatcgacag
    14521 cggcatcaac atggccagca ggccaaggat aaaaacaata gcaaacgacg aatgctgtcg
    14581 ggtggtcaca acgggctcct gaaagtcatt gaaaagttag acgacgctgg caatttcttc
    14641 ttcagttaac ggacgatatt caccgggggc taaatcagca tccagcgtaa taccgccaat
    14701 acgttcacga tgcagctcaa ccacgtggtt acccacggcg gcgaacatgc gtttcacctg
    14761 atgataacgc ccttcgctga tggtcagacg aacctgcgtt ggggtaatca cttcaagcac
    14821 cgccggctta gtgagatctt tttcgttatg cagctgcacg cctttagcaa attgctctgc
    14881 cgtatcgtca gctacaggtg attccagtgt caccagatag gtcttctcgc aatgatggcg
    14941 cggagaagta atgcggtgcg accactgacc atcatcagtc atcagcacca gaccggtggt
    15001 atcgatatcc agacgtcctg cggcgtgcag tttccacgct accggttcat ccagaaaata
    15061 gagcaccgtc gggtgatccg ggtcgtccgt ggagcaaaca tagccctgag gcttattgag
    15121 catgaagtaa cgtggaccgt gttgctgcgc cagcgggttg ccatcgtaag cgacatcatg
    15181 ttcagggagc agtttgaacg ctgcattacg aacgatttcg ccatcgacgg tgacacgatt
    15241 gccgcggatt tcacgcccgg caatagcacg gctaacgccg agttgctgtg cgataaattt
    15301 atcaagtcgc atgtgtgtga ttttgcctgt aaaaaacgga ggtcgggtaa tgacccgaaa
    15361 atctgaactg ttgtctgccc agtatagcgg tctaataact tccctcaagg gaaaaagaac
    15421 cgtggcatac tattagcaga ataatctacc tacgcgaaac catgattttt acactccgcc
    15481 catatcagca agaagccgtg gatgccacgc tcaaccattt tcgtcgtcat aaaacccctg
    15541 ccgttatcgt gctgcccacc ggcgcaggta aaagcctggt gatagcggaa ctggcacggc
    15601 tggcgcgtgg tcgcgtactg gtgctggcac acgttaaaga actggtggcg caaaaccatg
    15661 caaagtatca ggcgctgggg ctggaagccg atatttttgc cgccgggcta aagcgcaaag
    15721 agagccacgg taaagtggta tttggcagcg ttcagtcggt cgcccgtaat cttgatgcct
    15781 ttcagggtga attttcgctg ttgattgtcg atgaatgcca ccgtattggt gacgatgaag
    15841 agagccagta tcagcaaatc ctcactcacc tgactaaagt gaatccccac ttacgcctgc
    15901 tggggctgac tgccacgcct tttcgattgg gcaaaggctg gatctaccag ttccattatc
    15961 acggcatggt acgcggcgat gagaaagccc ttttccgtga ctgcatttat gagctgccgc
    16021 tgcgttatat gattaaacac ggctatctga cgccgccaga acgactggat atgccagtag
    16081 tgcaatacga tttcagccgc ttgcaggcac agagtaacgg gctgttcagc gaagctgatc
    16141 tcaaccgtga gctgaaaaaa cagcaacgca ttacgccaca tatcatcagc cagatcatgg
    16201 agttcgccga aaagcgcaaa ggggtaatga ttttcgccgc caccgttgaa cacgcaaaag
    16261 agattgtggg attactgcct gccgaagatg ctgcactgat tactggcgac aaccccggcg
    16321 ctgagcgcga tgtgttaatt gaagatttta aagcccagcg ttttcgctat ctggtcaacg
    16381 tcgcagtact gaccaccgga tttgacgccc cgcacgtcga tcttatcgcc attctgcgcc
    16441 ctaccgaatc agtgagtctt taccaacaaa ttgtcgggcg cggtctgcgt ctcgctccgg
    16501 gcaagactga ttgtttaatt cttgattatg cgggtaatcc tcacgatctc tacgcgccgg
    16561 aagttggtac accgaaaggc aaaagtgaca acgttccggt acaggttttc tgccctgcct
    16621 gcggttttgc caacaccttt tgggggaaaa cgaccgccga cgggacattg attgaacact
    16681 ttggtcgccg ctgtcaggga tggtttgaag atgacgacgg tcatcgcgaa cagtgtgact
    16741 tccgtttccg ttttaaaaat tgcccgcaat gtaacgcaga aaatgatatt gccgctcgcc
    16801 gctgccgcga atgtgacacg gtactggttg atccggatga tatgttaaaa gcggcgctac
    16861 gactgaaaga tgcgctggta ttacgctgta gcggcatgtc tttgcagcat gggcacgacg
    16921 agaaaggcga atggttgaaa atcacctatt acgatgaaga cggcgcggat gtgagtgagc
    16981 gtttccgtct gcaaacgccc gcccagcgta ccgccttcga gcagcttttt atccgcccgc
    17041 atacgcgcac accgggcatc cctctgcgct ggatcaccgc cgccgatatc ctcgcccagc
    17101 aagccttact gcgacatccg gattttgtcg tcgcccgcat gaaaggtcag tactggcaag
    17161 tgcgtgaaaa agtgttcgat tacgaaggtc gttttcgtcg ggcgcacgaa ttacgcggtt
    17221 aatggtagtt ttgattgatg tataaggcga ttgagtatag aatctcgccc gcttttgcat
    17281 acgcaaagca gatcacttac ctgttgctgg gtcgcctgta gcaggattaa tttaagagag
    17341 aaagaaatgt ttactatcaa cgcagaagta cgtaaagagc agggtaaggg tgcgagccgc
    17401 cgcctgcgtg ccgctaacaa gttcccggca atcatctacg gtggcaaaga agcaccgctg
    17461 gctatcgagc tggatcacga caaagtcatg aacatgcaag ctaaagctga attctacagc
    17521 gaagttctaa ccatcgttgt tgacggtaaa gaaatcaaag ttaaagctca ggacgtacag
    17581 cgtcacccgt acaaaccgaa gctgcagcac atcgacttcg ttcgcgctta attgctgaat
    17641 aagttgtaaa aaaccccgct ccggcggggt tttttgtatc tgcagattat gcctgattac
    17701 ggtattgcta ttttttgcag gccagataag gcgttcacgc cgcatctggc attcagaaca
    17761 caaaaccgat taattgccgc cagacgtccg gcgctgcaat tggtcgcgca aattcggcgg
    17821 cgtgccttta atggtcaagg tatcggttgc tggatcccag aagatgcgtt cacctaacag
    17881 catcgcatca aagttaatag tcaagccacc gccgctaccg gcaaacttcg tcaactgacg
    17941 cagtgtgcta cggtctgccg ggaagctctc ttccagttcg tagccttttt cagccgcaaa
    18001 ctccgtgaag ctcacttcgc ttacgcctgc cagctctttc gacagcgatt ccagttcgat
    18061 ctcttcgcct gcctgcagct gctcattaca gtagctataa acctgctggc gcacgttctg
    18121 tcgctctgct ttatccagtt gtgcttcggc agtgaaatca tcaaccgctt gcaacagccc
    18181 acggttttgc gctttcgcgt tcaatccttc gctggccccg aggaaatcca taaagaaatc
    18241 cgccactttg cgccctactc gccctttcag gaaagtgaga tagcgggtgg actctggatt
    18301 ggtttcccat tcggttaaat cgatacgcgc aacaatatcc gcatgattga tatcaagata
    18361 atgggttggg ttgatgtcga gattttcgtt aacgcgcata ctgctcaggt tgctcagaac
    18421 tgccaccagc aaatactcca ccgccagata acggtagtgg caaaacagca cgaatccacc
    18481 gtcagcaaaa ggatatttcg ctaattcgtc acgcaggcga ccggttgccg cgcggctaaa
    18541 tgccagaaag tcctcttcgc cctgacgctg taagcgcagc gtctgcgcca gttcactctc
    18601 ttcgctaaac aagccgtaag ctttattttt ggcgctatag acccgatgca gttctgccac
    18661 catctcaacg acggtttctg tcggttccag caatgaatcg cgcaacacca gctcaaggtt
    18721 ctgctcatca cgcttgataa gctggtgcag agcaatctgg ttgatatcca gactcatgat
    18781 aaactctcct ttaagaccgg gcggtattca accaccgcca gttaagcgac gcaataaaag
    18841 atggcggtca ttttacagaa ggcagcgtaa cgagaatgat ttaacgcata aaaaagcaga
    18901 aaaaaaaccg ttgctacggt aatatgttgc cctttcatca actaactgat ttcgatttat
    18961 gccacaaatt tcccgctaca gtaatgaaca ggttgaacaa ttgctcgcag agctgctcaa
    19021 cgtactggaa aaacataagg ctccgaccga cctttccctg atggtgttag gcaacatggt
    19081 cactaacctt atcaacacca gcattgcccc ggcccaacgc caggcaattg ccaactcttt
    19141 tgccagcgcc ttacagtcct ctatcaacga agacaaagcg cactaaggga aacagataac
    19201 aggttatggt aactcatcgt cagcgctacc gtgaaaaagt ctcccagatg gtcagttggg
    19261 ggcactggtt tgcactgttc aatattctgc tttcgctcgt cattggcagc cgttacctgt
    19321 ttatcgccga ctggccgaca acgcttgctg gtcgcattta ttcctacgta agcattatcg
    19381 gccatttcag cttcctagtg ttcgccacct acttgctgat cctcttcccg ctgaccttta
    19441 tcgtcggctc ccagaggctg atgaggtttt tgtccgtcat tctggcgacg gcgggaatga
    19501 cgctattact gatcgatagc gaagtcttta ctcgtttcca tctccatctt aatcccatcg
    19561 tctggcagtt ggttatcaac ccagacgaaa atgagatggc gcgcgactgg cagctgatgt
    19621 tcatcagcgt gccggttatt ttattgcttg aactggtgtt tgcgacgtgg agctggcaaa
    19681 agctgcgcag cctgacgcgt cgtcgacgct tcgcgcgccc gctggccgca ttcttattta
    19741 tcgcctttat cgcctcgcat gtggtgtata tctgggccga tgccaacttc tatcgcccga
    19801 tcaccatgca gcgcgctaac ctgccgcttt cgtacccgat gacggcgcga cgtttccttg
    19861 agaagcatgg cctgcttgat gcgcaggagt atcaacgccg tcttattgag caaggtaatc
    19921 cagacgccgt ttccgtacag tatccgttaa gcgaactgcg ctatcgcgat atgggcaccg
    19981 ggcagaatgt gctgttgatt actgtcgatg gcctgaacta ctcacgcttc gagaagcaga
    20041 tgcctgcgct ggcaggtttt gctgagcaaa atatttcgtt cacgcgccat atgagctccg
    20101 gcaacactac agacaacggc atctttggcc tgttctatgg catctcgccg agctatatgg
    20161 acggcattct gtcgacccgt acgcctgcgg cattaattac tgcgcttaat cagcaaggct
    20221 atcagctggg gttattctca tcagatggct ttaccagccc gctgtatcgc caggcattgt
    20281 tgtcagattt ctcgatgccg agcgtacgca cccaatctga cgatcagacc gccacgcagt
    20341 ggatcaactg gctgggccgc tacgcacaag aagataaccg ctggttctcg tgggtttctt
    20401 tcaatggcac taacattgac gacagcaatc agcaggcatt tgcacggaaa tatagccggg
    20461 cggcaggcaa tgtcgatgac cagatcaaac gcgtgctcaa tgcactgcgt gattctggca
    20521 aactggacaa tacggtggtg attatcactg ccggtcgggg tattccactg agcgaagagg
    20581 aagaaacctt tgactggtcc cacggtcatc tgcaggtgcc attagtgatt cactggccag
    20641 gcacgccggc gcagcgtatt aatgctctga ctgatcatac cgatctgatg acgacgctga
    20701 tgcaacgcct gctgcatgtc agcacacctg ccagcgaata ttcgcaaggt caggatttgt
    20761 tcaaccctca acgccgtcat tactgggtta ctgctgcgga taacgatacg ctggcaatta
    20821 ccaccccgaa aaagacgctg gtgctgaaca ataacggtaa ataccgcact tacaacttac
    20881 gtggtgaaag agtgaaagat gaaaaaccac agttaagttt gttattgcaa gtgctgacag
    20941 acgagaagcg ttttatcgct aactgattaa ttataaatca gttagcgaaa tatcttactt
    21001 gcaatcggtg tggaaaacgg tagtattagc agccacgagt cggcacgtag cgcagcctgg
    21061 tagcgcaccg tcatggggtg tcgggggtcg gaggttcaaa tcctctcgtg ccgaccaaaa
    21121 atcccaagaa aaaaccaacc cttacggttg gtttttttat atctgcaatt aattcgataa
    21181 acagaccgtg acacatcaca gcctgtttat tttctgttat cagaacgtcc agaccacacc
    21241 cgcctgagta ttccacggcg attctacccc tgcgccattg ccatagctga ctgacaaatg
    21301 accgcttaac gtcggggtaa acgatgacct tatcccagcc tgataaacgc cactggttcc
    21361 agccacatca ttattaaatt tgccatcgtc attaactttc acctggttag aatcggcgta
    21421 ttcctgacgc acggcggctt tcagccaggg ttccagcgtc gtaccgtttt gcaggtccat
    21481 gtgatagctt accgccgttc ccgcttcagc gcgtaatatc cgggtatttc ccacatccgc
    21541 gcgcatgccg tttgataacg tgtagtcctg accatctgtg gtaaagccgg taaaggccag
    21601 atagggtcta acactccaca atccgtcaac ccaacggaac ccgctctcaa catgagcacc
    21661 cgcgccgtta ctattgtaat cgccaaacgc tgttgcccca ttactcatct tgccatggat
    21721 ggtgttggca aaacggtcaa ctttcaccac cccatcaaca taggcaccgt tctgatgctc
    21781 ccaaccggca taagccccca gggtatagct atcgatatta ccttttccgc cgcgatcaaa
    21841 accaatatca gaatgagagt aaccaaagaa caagccgcga attgtactgc tttcttcacg
    21901 ggagaaacgg ctatcgatac cgagcgtcag gcccgtcaat gtttgctcaa aaccagctcc
    21961 cgcatcagtg gtcacgttgt tgcgggtgtt aattgccgaa ctccacatca ccgtatcgta
    22021 actaacgcct tttacgctac caagacgctc acgcacggtg tccagttctg catcaaatac
    22081 cagcggttgt gcggctgcca tgttcagcac atcagtggtt gaaggggtaa tttgcgcgcg
    22141 attctctgcg aggctccagc tatggttgcc attatccagc aaggtatatt catacgtacc
    22201 gatatcaaca acgcctccgg cattgcccaa cgtaaatgca gcatcaccgc cgcccgttgt
    22261 taccagtgta aggctatctc ctgctgccgg gctggcaccg gtgtccgtca cgaatatttt
    22321 gaaatcacct gttgcctgac cggtgacgtt gagctgatcg ctctgatgat tggccatatc
    22381 ggtacgcata taaaaattgc cgtttcccga cagggtattg gtggtcaacg taatgaaatt
    22441 ttccgcactt gccgtcacag atgaacgagt cactggctca gccatcataa tcacatttgc
    22501 gccggacatc gcaaggttgg ctaagttgag gcgagtgttt tggttcgtcg tagtgatatc
    22561 ggcaatgatg gtccatgcgc tattttcgag cgaaacgtcc gctttgctgg tatccacggc
    22621 accatgcgtt ctaaaagaag cgccctcaga aaccactacc ttacccacca acatagcggg
    22681 tgcataattt atttgcggct tcatgacctc aagaatgcca tcattccctc tgactgacac
    22741 gttaaccatt gtgccagccc agacattagc gcggccattg ttgattacca tattttcaga
    22801 gatggctttt gaggaatacg tgatacttcc attctggtct gatcgaccga gatcatatac
    22861 cgcatttgcc tgcactttcg taccagtatc ctttcccagc gattgcatag tggcatgctc
    22921 atcaaggata gtgtcaatgg cctgcgtgtc ctccataaca atgagcccgg aaccatcatc
    22981 cagttcataa ttttttgaca caccatcctt tatactaaat tggcctttac tgtttgtacc
    23041 actcacttcc agcgcattcg ttgagacaat taatttcccg cctgcttttt tatcaacgcc
    23101 agttgcagtc ccgccatcca taacctccag taagccgcca ctatccacag tagtattata
    23161 agcgaagtca ttttcttcaa ctcgcaaact tccgccgttt tccaacaaca tattattagc
    23221 aataccattc ttgatatcga actgaccgag acggtttgtt ccgaatacct ccatggcccg
    23281 cgtggttgtt ttaatagcac cgcctgtttt ctgatctacc gcaagcgcaa atcccccctc
    23341 tttaacctcc agtagtcctc ccttttcgat agcggcaaaa acggcccccc catctttttc
    23401 aacgattaag cgcccgcctt taatcgtggt atcattcgct gggccataaa cctgtagcac
    23461 accattattg agggtagtct ggtttgccgc gccacccatt tgaacaattt gataaccatc
    23521 atttaatact gagtattctg cggtaccatt catttcaatg tattgatacc ctccgttaat
    23581 ttcagtatta ctacttacac caaattcttt tatatgctgt tctccgccag gattgatttt
    23641 cgtattattg gcggtcccat aaaccagctg gatatctttt tccaggataa caccatcaac
    23701 tgtctcacca tactcaactg ttgatgccaa tgctatgggt gaatataacg ctgtagccac
    23761 cagaacagat attgcggaac gacaaagaga aacagaacca gattgatgca ttgagctttc
    23821 atcctatgaa attaattgca gttaaaagca ttgggtacag aaaataccca tagctccata
    23881 cccggagtca gtttttaaaa actgtttaaa gaaatgcaca agtattgtga ttgatttttt
    23941 agttgttttt cttgatgaga agctgatgca aaattccgtc tttataataa aaatgatgcc
    24001 aaagcgaacg acaaggttgt agttttcact acatgtccat acataaaatg gggtaacatt
    24061 cacgcgcctg gtagcgttac caacgctacg ctcaaacata atgattctaa taaaacctca
    24121 ggagactact atgcctgaag caacgccttt tcaggtgatg attgtggatg atcatccact
    24181 tatgcgacgc ggtgttcgtc agttactgga gcttgatcct ggctttgaag tggtcgccga
    24241 agcgggcgaa ggcgcgagcg cgatcgatct ggcgaataga ctggatatcg acgtgatctt
    24301 gctggatctc aatatgaaag gtatgagtgg cctggatacc ctcaatgcct tgcgcaggga
    24361 tggcgttacc gcgcaaatta ttatcctgac cgtatccgat gcctccagcg atgtctttgc
    24421 gctgatagac gcaggcgcag acggttatct gttgaaagac agcgacccgg aagtattgct
    24481 ggaagcgatt cgtaccggag cgaaaggcag caaagtcttt agcgaacgcg tcaatcagta
    24541 cttacgtgaa cgtgaaatgt ttggcgcgga agaagatccc ttcagcgtgc tgacggagcg
    24601 cgagctggat gttctgcacg agctggcaca ggggctgtca aataaacaga ttgcatcggt
    24661 gttgaatatt tccgagcaga cagtaaaagt acatattcgc aatctgctgc gtaaactcaa
    24721 tgtccgctca cgcgtggcgg ccaccattct gttcctgcaa caacgcgggg cacaataaaa
    24781 atagcccgat ggatttatca tcgggctgag atttatgaca aacgcaaaac tgcctgatgc
    24841 gctacgctta tcaggcctac gtggatcgat caatttattg aatttacaca attttgtagg
    24901 ccggataagg cgttcacgcc gcatccggca taaactaagc gcactttgtc atcagtctag
    24961 cccgatggca tcaccatcgg gcctcttttt atttactctc ctgcggcgac aaatgttgca
    25021 tcgcctgcgc gatactacgt tcaatcaccg cacggcgagt atcgttggca ggtaagagtt
    25081 tcaacatcat ctcccacgcg gcaacggctt cgccaaatcg ctgctgctca aaggcattaa
    25141 acgcatacat gcttagcaca cggatattgc tatggtccgt tctcaccagc tgacgtagca
    25201 gttcaccgcc gaggcggttg tcgttgggat cagatgaacg agtcaacgct tcagcgtatc
    25261 ccagtgcggc atcgctgttc ttcggatcga ggcgatacgc agtagcgtat gcatcggtgg
    25321 cgatactggc gttacccagc gccatgccaa cgcggcccaa cataatccag ccttctatat
    25381 ctcccggatt tttttgcagt tgagtacgca tccccagcgc aaggcgcgac atctcttctt
    25441 cgttgagcgg atcggctttc gggtccagcg ccctgtccag caacgccgga gcctgtgccg
    25501 tggcctgctg ccagattttc acctgctgat aattgccagt ctggtagtag ctgacgccag
    25561 ccacaattaa cgccaccaca atacccggca gataaacaac atatccggca cgcttacctt
    25621 ccggcacgct ttgttcagga aacgcttccg gcaccacgcg tacccgacgc cgcgagcggg
    25681 cgtatatgac ccaaccgcca atgccaatag ccactactgg cagcacccac agcagcacgg
    25741 tcagcggcgt taacggcgga tcgtaagtga cgaagttgcc gtaacgcgcc accatataat
    25801 cgacaatctc ttttttactt ttaccttcct gcatcagttc atacactttc tgacgcaggt
    25861 cggtggcaat catcgagttg gaatcggcaa tgctgttgtt ctggcatttc gggcagcgca
    25921 gttcttcagt gagctgacgg aactgttgtt cctgtgcttc atctttaaac tgcaacacgt
    25981 cgatggtcgc cagcgctgag ccggagatca tcagcatcag cacgcccaat aaaaacctca
    26041 ttgtgcggcc tccttactgt atttctccca cagcggcttg atctcttctt cccagacgcg
    26101 aggattgaga tcgcccgcat ggcgatagcg aatgataccg ttgccgtcaa taaggaaggt
    26161 ttctggcgcg ccatagacac cgagatccag ccctaacatg ccgtcgccat caaacaggct
    26221 tagcgcgtaa ggattgccca gctctttcag ccagctgatt gccttctggc gatcgtcttt
    26281 atagttcatg ccgaccacgc gaatgccctg cgctgaaagc tgattcagat attgatgttc
    26341 cgcacggcag gtcggacacc aagtcgccca gacgttaagc agtactggtt tgccctgagt
    26401 cagcacatcc gcctgataaa actgccccgg attgtccagt gattcgagac gaaacttcgg
    26461 cacaggcttg ccaatgagcg ccgattccag attggtcgga tcatcccctt cggcattacg
    26521 cgccagctgc cacagcagcg ccgcggcaat cgccaggaag ataatcaacg gaattaacaa
    26581 tactttgcgc ttcatacggc ctccggcgca gttttttgcg gactcacgcg cttacgatag
    26641 cgaggatcaa acagacacag cagtccgccc aacgccatca tcagcccgcc cgcccaaatc
    26701 cagcgaacaa atggtttgta gtaaagacgc acggcccacg cgccgttttc cagctcttca
    26761 ccgagcgcgg cgtacaggtc acgcgtgatg ccgccgtcaa ttgccgcttc ggtcatcatc
    26821 gacccggcag tgttgtaata acgtttttcc gcatacagca ccgtttccgg tttaccctct
    26881 cgcgttacgc caatagtcgc cacaccgccg cgccagttcg ggccagtcac ctctttgaca
    26941 tcacggaagg tgaagcgata ttcatgaata tcgacgctat cgccggactt catgcgcaca
    27001 tcacgctcaa cgctataatt ctggctaaag gcaatgccaa caattgtcac tgccagcccc
    27061 aggtgagccg ccaccatccc ccaataactg aaggtggttt tcgtgccgcg tgaaatacgc
    27121 agcgcagctt ccgcagttgc cagcaccgca atccagcagg ccattgccag gccgagcacc
    27181 gtcatcgcca caactttgct ttcgaacagc cacggcaaca gcagagacag caccagcgta
    27241 gagatgaagg cgataatcaa taaattgcgg attttacgtg ggcgatcgcg cccccagcgc
    27301 accagaggac cgacaccaag cagtagcgca aacggcacca tcagccaggt aaacatggtg
    27361 ttgaagaacg gttcgccaat cgaaatactg cccagtccaa gttgcttgtg caccagcggc
    27421 agcagcgtcc ccagcaacac caccagcatc gcggcgacca gcaaaacgtt gttcgctaac
    27481 agcaaagatt cccgcgacca cagcgcattg tttacgcgtg agcgaacttt gtgtccacgc
    27541 gcggcaaaca gcagcagcga accgccaatc accagcacca taaaggcgag gataaacata
    27601 ccgcgcgccg gatcagacgc aaacgcgtgt accgatacca gcacgccgga acgcacgagg
    27661 aaagtcccca acagacacaa cgagaaggca ctgattgcca gcagtaatgt ccacgctttg
    27721 aagctggcgc gttgttcagt gaccgccagt gagtgcatca gtgcagtccc caccagccac
    27781 ggcataaacg aggcgttttc taccggatcc cagaaccacc agccacccca gccgagttcg
    27841 taataggccc atgcggaacc gagcacaatg ccgagcgtta ggaaaatcca cgccgccagc
    27901 gtccacgggc gagtaaaacg cgcataagtg ctgtccagac gcccgctcag caaagaagca
    27961 atggcaaaag caaacgccac cgagaaaccc acgtacccca tataaagcag aggcggatgg
    28021 aagatcagcc ccggatcctg taacagcggg ttaagatcgc gcccttcaat cgggaagttc
    28081 ggcagcgtgc gtgagaacgg gttagaggta aagagaatga acagcaaaaa gccgacgctg
    28141 accatcccca ttatcgccag tacacgggcc acaatatcca gcggaatacg ctgactaaaa
    28201 atcgccaccg caaaggtcca gccgctcatc agcagcaccc acagcagaag cgaaccttca
    28261 tgcgcgcccc aagtagccgc cacgcgatac cacaccggaa gctgggtatt ggagttgctg
    28321 gcaacatagg tgacggtaaa gtcgttgacc acgaaagcat tgaccagcac cagaaatgcg
    28381 ccagccacag acataaacag cagccaggca aacaagcggg aagacgccat catgcgcgca
    28441 tctccgcgcg ccacgcccca tagcggatac acggacagca gcagcgcaat ccctaacgcc
    28501 aagcacagca gcccgtttcc aatttccggc atcatgatgc tgggtcctta taaacactcg
    28561 ccgggcgacg gtggttagct tccatcgctt tctcaacttc tggcggcgtg tagttttcgt
    28621 cgtgtttcgc cagcacttct ttcgcgagga tatgattgcc tttttccagc tcgccctgca
    28681 ccacaacgcc ctgcccttca cggaacagat ccggcaaaat gccttcgtaa gagacatcca
    28741 ctgagccttc agcatcgtaa atggtgaagg tcactttcag cgaattggga tcgcgctgca
    28801 cgctacccgg catcaccatc ccgccaacgc gcagacgctg accgacttcc ggcatctgct
    28861 gagtttcacg cttgccgtag agaatttccc ccggcgtata aaagagatcg atattcgagc
    28921 gcagcgcata tagcaccaga ccgatagtca gcgctagccc tgccaacacg gcacaggcaa
    28981 tccacaagcg gtttttacgg cgaatattca tgcagcctcc tgctgttgcg cagcacgtaa
    29041 acgcgcctca cgcgcccgct gttgcgccac accacgcaga attgcgcgat gttgcatcac
    29101 cgagtgcacg accaaaacca ccagcggaat aacggtcatc accaccgcca gccagacaaa
    29161 aaaggcgtaa ccgcccattg cgaaaaattc attccaggaa gcaaatgcag gggtcattta
    29221 cggcctcttt tcagtatcag ttcactcacc cacggacggc gtttttccat cagcaaaatc
    29281 aaattacgca tccgcatcag cgtcagcgtg gcagacagga gcaggaagcc aaaaatcgac
    29341 cagcgcagcg gcgaacgcat cgccggatcg atactttgct gcatccgcgt tgatccctga
    29401 tgcagggtgt tccaccactc cacggagtaa tgaataatcg gcagattcac cacgccaatc
    29461 agcaccatga tacctgccgc acggcccgcc agacggcggt cgtcgaaggc gtgccacagg
    29521 gcaatcacac ccacatacaa aaacagcagc accagttcag aagtcagacg tgcatcccat
    29581 acccaccagg tgccccacat cggttttccc catgcagagc cggtaaccag ggcaataaag
    29641 gtaaacacgg caccaatggg ggccatcgcc gccaccgcca ggttggccat tttcatctgc
    29701 cagacaaggc caataaacgc cgccaccgcc attgatgcat aaatgcccat cgaccagatc
    29761 gccgcaggca catgcaggta gataatgcgg tagctatttc cctgctgata atcagccgga
    29821 gcaaagccga atccccagat ccagccgacg gtaagcacga ccacactggc aattgccagc
    29881 cacggtataa accagccaca gatttgatac agccgtggtg ggatcgccag ttgatgcagt
    29941 gttttccaca tagtttcgat accagactcg aacaaaaatc agtaatccag cgttattgaa
    30001 tgctgattcg taacgctgcc gccgtcgcaa aaggacttaa tgtcgcggtg cctgccagca
    30061 acgcgcctaa aattgccaga tacccatcaa cgggcaaatg catagaagcc gcgtccatcg
    30121 cggcggtggc aaagatgagt aatgggatag tcagcggtaa caccagtatg ctgagcagca
    30181 caccaccgcg cttaagtccc actgtcagcg ccacgcccgg tgcaccgaga aagccaagcg
    30241 taggcgttcc cagcagcagc gtcagcgcca tcacttgcca gccataaaca tccattccca
    30301 gtagcattgc taccagtggc gaaaggatga gtaacggcag accggttacc atccagtgcg
    30361 ccatcacctt cgccagcaca acggcgggca agggtaacgg caacaacatc aattgttcaa
    30421 gactgccgtc ctgcaaatcg tcacggaaca gtcgttccag cgccagcaag gatgaaagca
    30481 gcgcagcaac ccagataatg cccggtgcaa tacgcgccag cagttgcggc tccggaccga
    30541 tactgagcgg aaaaagggta attacaatca ggaagaacca cagcgggttg gcgatttcgg
    30601 cgctatggcg aaacgctacg cgcagttcaa gacggaaaat acgccagaac atcatgcggc
    30661 cctcgtttgc gtcagtgaaa tgcggcgaat tttactttca gcaacgttga gcggctggtg
    30721 ggtagtcaga atcacaatcc ccccctgctc cgtatgctgc gccatacgct gggtcagacg
    30781 atcgacgccg ttaacgtcaa tcgcggtaaa aggctcgtcg aggatccata atgtggcacg
    30841 ggtcaaccac agacgcgcta aagcgacgcg gcgttgttgc ccggcagaaa gttgatttac
    30901 cggaatatct tcgaatccgg caagcccggc ctgcgccaag gcttccagac attgtgcggc
    30961 atcgccatcg cgatgataaa agtgcagatt ttctaacgcc gtcagccggg ttttgatccc
    31021 cggctgatgg cctatccata acagggtttg atggtagctg tcgcgtacct gatgcaaggg
    31081 ctgcccttgc cagagaacct cgccagcgtc agggcgagac aaccccgtca gcaaacggag
    31141 aagcgttgtc ttccccgcgc cgttgctacc ggtgatttgt acccactctc ctgcgttcag
    31201 cgtaaatgac aagccactaa ataaggttcg ttcatcccgc tcacaaagta actctctggc
    31261 ttcaagcata cccacgcaat aaccctgtta aaaacctggc tcgacttcac gcatatcggg
    31321 cagcttgtgc gcgatccctt tatggcaatc aatacaggtt tgcccatctt tcaccgcctg
    31381 gtcatgcatc ttcgcggcaa ccgatttctg ggcggttgta tccatatact cgaagttgtg
    31441 acagttacgg cactcctgcg agttattgtc cttcatgcgc cgccactcat tctgtgccat
    31501 cgtcagacga tgagcttcaa atttctgcgg cgtgtcaata acgccaaaaa ttttaccata
    31561 cagctcttta cttgctttga gcttgcgtat catcttcggc acaaactcgt gcggaacgtg
    31621 acaatccgga caggtcgcac ggacgccgct acggttgttg tagtgcacgg aatccatgta
    31681 ttcctgatac accgtgttgc gcatttcgtg gcagctaatg cagaactctt cggtattggc
    31741 tttttccatc ccggtgttaa agccccccca gaagacgatg ccgccaacaa aaccgatcaa
    31801 cagcagcgtc cccagcgcca gatggctggg ggtacgccac catttccaca ggcgcttaat
    31861 cagaccaggc ttacggtcag aatttcccat aataacctct tatttcccgt aaccttttga
    31921 tggggtaaag gtattcccca cgattggcgc ggtatcggcc tgcggtacgt gacattgcag
    31981 acagaaataa cgacgcggag ccacttccgc gcccactttg ccgtcgctgt ccataaagtg
    32041 agtaggactg atacgcggcg cgccagtggt gcgatagctt tcgacaccgt ggcactgcaa
    32101 gcagcgattg gtattggtcg ttacctgata accttcaacg ctatgcggga tcattggcgg
    32161 ctgattcaca tagttcagcg gcatccgatc ctgctctttt ggcatccgaa tggccccttc
    32221 ctgcgtcccg gatacttccg gcgactggct aaaatcgacg ccattagccg cccaaaccgc
    32281 gccgcttacc accagggcca gcatcgccgt ccattgacac agcgctttct tcaggtcatg
    32341 gcttttcatg atttcgctcc cgaactccat cgtgtagtta ttgtaaatac atcctcagaa
    32401 cagacatcca cgcagcgacc gcacgtcatg caatcgcggc tggtgacctg caccgggctt
    32461 tgttcatcca gcaccggggc acgtagcaca tgcggttccg ggcaaacatg aaaacaatcc
    32521 atacagcggt tacatttctg ccgatcgctg gccgcaacgg taatcacacc tttgctaccc
    32581 agcacgccat acagcgcgcc aaccggacaa atgtgcccgc accagccgtg ttcaacgacc
    32641 agtagatcaa ataaaaacag cgcgagaata agcagcgcgc cgctgccgaa gcccattacc
    32701 aggctacgcc ccatcagaga aacggggttg atccattccc aaatgagcgt gccagttaag
    32761 gctgatccca ccagaatgac caccaacagc acgtagcgaa tgtggcgagg gatcgtcgca
    32821 gactgattga ggtcaaagcg cctgcgtaac cagttcgcta agtcggtaat cgggttcagc
    32881 gggcagaccc agctgcaaaa taatcgcttt cccgccaggg cgtagagcac ggtgataatc
    32941 accgcaccgg tcaacgccac cgtagcaggc agatgaccac tggcaagact ttgcagcgtc
    33001 atcagcggat cggtcagcgg aacggtgtcg aataacaggc tactgctgta gttgccgtgc
    33061 aagatccaca caccaaacca tggaccgctc aaaaacatcc ccagcacgaa gaactgacaa
    33121 aggcgacgta acaccagcca acggtgactg cgccaccagc cttttttctc cagcgcctcg
    33181 cgcccggcgt cacgtttacg atttgccatt gttcccctcc agccagccga agcggtaatg
    33241 gtgacctaac tcccctttcg ccagtgacag cggtaacacc ttgattgccg gttgttccag
    33301 cacgcatact ttttcgcact taccgcaacc ggtacaggcg tcgctgtgaa ccgtcggcag
    33361 aaagcgggcg tgcttaccgg tacgcgtgtt gcgctccagc tccaaggtga tggcctcatc
    33421 aattttcggg cattcgcgat aacaaacatc gcagcgcagc ccctgaaagt tgagacagtt
    33481 ttcctggtcc accagtaccg ccagccccat ccgcgcgtcg tcgatcgatt caatctcacg
    33541 atccagcgca ccgcttgggc acactttggc gcacggaatg tcctcacaca tttcgcaagg
    33601 aatatcccgt gcgacaaaat atggcgtgcc cgccgacaga ccagaggcca gcgtcgccag
    33661 ttttaaggtg tcgtaagggc aagcctgaac acactgaccg caacgcacac aggcactggc
    33721 aaaggcgttc tcgtttatgg ctccgggcgg gcgcaaccgc acgccagatg cgcgtgcggt
    33781 ttgctgttgt aaccccagcg ccacacccac ggcagccagc ccgcctgctg tgcgaacgac
    33841 atcgcgcaga aagcggcggc gaccattttg aggtttcgct gaccgggaca taatggctta
    33901 caccttctcc agtttgaccg cgcacttctt gaagtccgtc tctttcgaga gcggatcggt
    33961 cgcatccagc gtcagtttgt taaccagctg tgcggcgtcg aagaacggca tgtacaccag
    34021 gccctgtggc ggacggttac gaccgcgcgt ttcaacaatc gagatcactt cgccacggcg
    34081 agaaaccact ttcactttgt cgccacggcg cagatcgcgc gctttcgcat ccagcgggtg
    34141 aataaacaag accgcttccg ggaaggcgcg gtgcagttcc ggcacacggc gagtcatact
    34201 gccggtgtgc cagtgctcca gaacgcgtcc ggtagagagc cacaggtcgt actcttcatc
    34261 cggtgcttcc gccgccggtt cgaacggcag tgcgaagatc accgctttgc catccggttt
    34321 accgtagaac ttatagcctt cgcccgcttt cacgtacggg tcgttacctt cgctgtaacg
    34381 ccactgtgtt tctttaccat tcaccaccgg ccagcgcaga ccgcgcgctt tgtggtagtc
    34441 atcgaacggt gccagatcgt gaccgtgacc gcgaccaaac catgcgtact cttcgaacag
    34501 ccctttttgc agatagaagc ccagctcgcg ggattcatcg ttcagctgat cttccgccag
    34561 ttcggatacc gggaatttgc tcacttcggg ggtggcatac agaacttcgt acagcgtttt
    34621 gccacgcagt tccggtttct tcgccagcag ctcttccggc catacttctt cagttttgaa
    34681 gcggcgggag aactggacta actgccagag atccgatttc gcttcgcctg gcgcctgtac
    34741 ctgctgacgc cagaactgag tacggcgttc ggcgttaccg taagcgccct ctttctctac
    34801 ccacattgcg gtcggcagga tcaagtcggc ggccagcgca ctgactgtcg gatacggatc
    34861 ggagacgatg atgaagttgc gcggatcgcg ccagcccggc atacgctctt cattgatgtt
    34921 cggcccggcc tgcatgttgt tggtacacat ggtccagtaa acattcagct tgccgtcttt
    34981 cagcgcacgg tcttgtgcca ccgcatgaag accgattttc gccggaatgg tgccgctcgg
    35041 gatattccac ttcttctcac agatatcacg atgtttctcg ttagttacca ccatgtccgc
    35101 aggcagacgg tgagcaaagg tgcccacttc acgcgcagta ccacacgcag aaggttgccc
    35161 ggtcagggag aacggaccgc aacccggctg ggaaattttg ccggtcagca ggtgcaggtt
    35221 gtagaccagg ttgttagccc acacaccacg ggtatgctgg ttgaagccca tcgtccagta
    35281 ggagatgact ttcttgttcg gatcggcata cagctgcgcc agttgttcta actggtcttt
    35341 cggcacgccg gtcatttcag cggttttttc cagcgtatac tcagcaacga aggctttgta
    35401 atcttcaaag ctcatcggtt cggaggcgtc agaacccgga ttcttcgctg ctttttccag
    35461 cggatgggtc ggacgtaaac cgtagccgat gtccgtcgcc cctttgcgca ggttaacgtg
    35521 cttgctgaag aagtcctgat ttatcgcatt gttttgaatg atatagttgg cgatgtagtt
    35581 caggatcacc aggtcagatt gcggcgtaaa gatgatgccg ttatccgcca gctcgaagct
    35641 acgatgctgg taggtagaaa gcaccgccac ggtgacgttc tggttagaga gacgactgtt
    35701 agtgatgcgt gaccagagga tcgggtgcat ctccgccatg ttagcgcccc acagcacaaa
    35761 cgcgtcagcc tgctcgatgt cgtcatagca gcccatcggc tcatccatac caaaggtacg
    35821 cataaagcca actactgccg acgccataca gtgacgtgcg ttcgggtcga tgttgttcga
    35881 acggaagccc gctttgaaca gcttggacgc ggcataacct tcccagatag tccactgacc
    35941 agaaccgaac ataccgatcg attccggccc tttttctttc agggcggttt tgaatttctc
    36001 ttccatcaca tcgaaggcct gatcccaggt gattggggta aattcgcctt ctttgtcata
    36061 tttaccgttt ttcatacgca gcagcggctg cgtcaaacgg tctttaccgt acatgatctt
    36121 gggcaggaaa tagcccttaa tgcagttcag gccacggtta accggtgcgt ccgggtcgcc
    36181 ctgacaggcc accacacgcc cctgctgcgt tccgaccaga acgccgcaac cagtaccgca
    36241 gaaacggcac ggcgctttat cccatttaat ggcttcctgc tgaccaacaa cggcgcgggc
    36301 aacgcccggc acgctgagac cggcagccgc cgcagcggcc gcaacggcgt tagctttcat
    36361 aaagctacga cgactgagtt tcatggtgtt tcctcacctt gctcttcctg ctggtgataa
    36421 accagcgaca ccgccagcac gccctctacg ttgcgtactg actcaatggt ttggatcagc
    36481 gtttcgctgt cttctgcttc caccaccaca atcaactgac cgctcggcgc gtcgctgaca
    36541 gcaacttcac agccgggaaa ggcgttcagt tgggtgctga tgtctgaaat tcgttcgctt
    36601 ttggcctgca cgaccaggct gcaaacttgc cagttagtgt gcatggagat actccgcagt
    36661 tatggctgat accggacagc tggctgcaca tgctccacat ccgttgcaaa gttggctatt
    36721 aagttgcgcc tggtagatcc cggagagcgt cgggcgaaag ataattgcca ttggctcaca
    36781 gctatcctga cagcggcggc attcaactga ctgatacgcc aggcacgcgt ccccgatggt
    36841 gaactgcaaa tcccaggccc tggtgtggcg cggagaaaat agcgattcgg ggcaagcctg
    36901 cgcgcaggcg taacagaagc tgcactcgtt atttttgaaa ttaacgctcg gatagccacc
    36961 cgtgccgcgt tgcagaatgt tgttttcaca agcattaata caggcgtcgc agcgggtgca
    37021 atgggtcaga aaatgagatt catcaccgga ccagggcgga cggataccgt tactggcttt
    37081 gcgccagcaa ccagtgagta tgccccgacg ggatgcatca atcttcacat tgaccttcca
    37141 tcattaacgc gctctgaaaa ttgagagcga ccaaatagac cgcataatta ataagccatt
    37201 tttatagccg ctaagatatt aaaggatgtg tcaaagatgc ataccccgat cggggtaaaa
    37261 cctgtagcag gatcaaaaga gtggcgggaa gcgtggcaaa aacgggcttt tgctcacatt
    37321 tcaaatggtt ataaacatat ttatatagcg attaattcac cagagatatt tctgctggtt
    37381 tgctccctca tcagaattta acactaaaag agcaggtaaa attgtctgaa tgttctttaa
    37441 gttattcata aagcaaatta ataaatctga tgaatatgtt aaccttcagc gacatcatcg
    37501 gtgaaaacct ataaatgaag aaggaaagca aaaaaatgaa gaccattcta cctgaagtat
    37561 tgtttgccgc tttcgctacc acttccgcct gggcggcaga aagcgtccag ccactggaaa
    37621 aaatcgcgcc ttatccacaa gctgaaacag gcatgaagcg tcaggtgatt cagttaaccc
    37681 cgcaagaaga tgaatctacc ctgaaagtag aactgttaat cggtcagacg ctggaagtcg
    37741 attgcaattt gcatcgtctc ggcgggaagc tggaaagcaa aacgctggaa ggctggggct
    37801 atgattatta tgtctttgat aaagttagct cgccggtttc cacgatgatg gcctgcccgg
    37861 atggcaagaa agagaagaaa tttgtcaccg cgtatctggg cgatgctgga atgctgcgtt
    37921 acaacagcaa gctgccgatc gtggtgtata cgccagacaa tgtagatgtg aaataccgcg
    37981 tctggaaggc ggaagagaaa attgacaacg cggaagttcg ctaaactgtt gtgaagtacg
    38041 gcaccccgtt ggttggataa ggcggttacg ccgcatccaa catctaacgc ccgagccggt
    38101 tgcctgatgc gacgctggcg cgtcttatca gtatacgcca catcattaca acgcaatatc
    38161 cgccacttct ttttgaacgg gttgcggttt caactgcggt ttcggcgtac tatctgctgc
    38221 ttgcggtttg tcgtatttca gccccagcac ttcgctggtg tactgcaact cgcgttctgt
    38281 tgccgctaca tcaccgttca gcttgcgtcc ataagacgga acgatcgctt tcaacgtagc
    38341 ctgccattgc gggctggaaa cgcgatcgcc aaatactttt tccagtagat tcaacataat
    38401 cggcgcggcg gttgacgccc ctggcgatgc ccccagcagc gccgcaatgg tcccttgctg
    38461 gtcactgacc acttcggtac ccagacgcag tacgccacct ttctcggcat cacgcttgat
    38521 aatctgcaca cgctgccccg cttgccacaa acgccagtcc tcttttttcg cttgcggata
    38581 gtactctttc aacgcttcaa aacgatcctc ttcactcaac atcacctgac tcaccagata
    38641 tttcaccaga tcgaaattat ccagcccgac gtgcatcatc ggcatcacgt tagaggtggt
    38701 ggtggaactc atcagatccc acaatgaacc gtttttgagg aatttggttg agaaggtggc
    38761 aaatggccca aacagcacta cgcgtttacc gtccagaaca cgggtatcga tatgcggaac
    38821 cgacatcggt ggtgcgccaa cggatgcttt accgtaaact ttcgccagat ggcgattaac
    38881 cacgtctgga ttttccgaaa caaggaactg tccgcccacc gggaaaccgg cgtagtcttt
    38941 cgcttccgga atccccgatt cctgtaacag cttcagcgcc gcaccgcccg cgccgataaa
    39001 gacaaatttg gcgcggatgt tctgtgcagt gccgtttttc agatcggcaa cggtaacggt
    39061 ccaggtgtta tcgtcattac gctttagggc gcggacttcg ctgctgagtt gcagcgagaa
    39121 gttagatttc ttctgtaagg aagcaattaa ctggcgggtg atctcaccat agttcacatc
    39181 agtgccaatt tctgttgcgg atcgcgccct tccatcacta acggtgccca ctctttgatc
    39241 tgcgcgtgat cttcagagta acgcataccg cgaaacagcg agctttgttg caacgcggcg
    39301 taacgggcgc gcaggaaatt aacgttatcc tcgccccaga caaagctcat atgcggaacg
    39361 gtattgataa atgaacgcgg agtacgcagc acgccgcgct cgacctggtg cgcccagaac
    39421 tggcgggaaa tctgaaatgc ttcgttaatg gcgactgctt tttcaatact gatgctgcca
    39481 tcggcgtttt gcggggtgta gttcagttcc atcagtgcag aatgcccggt tccggcgttg
    39541 ttctagccgt tcgaactctc ctgcgcgccc ccctccaggc gctccaccat ggtcatcgac
    39601 cattcaggct ccagctcgcg tcaataggtc cccaacgtgg cgctcataat gccgccgcca
    39661 atcaacagta catcagtttc ctgctcctcg gacgctttcg cttttgccgc catcgaaacg
    39721 gcattaagcc ccacggccat cgagaagagc atggcagtca cttttttcat cttgttaatg
    39781 ccttactttt agtcgcttta ttgcaggtga gatttgcgcg gcatcaacgg taacacttaa
    39841 gtaacaacat ttaaataatg tttataaatt atattctaat tttagaaaca ttataatttc
    39901 gttgattaat tatagggtta ttagaaatga agggattttt agcatattga cctgacggca
    39961 gcagtccgcc aggtcagcgt cgtaatatta tgccgtccgg gcaacggcat cacgcgaagc
    40021 ggcatcgcgc tcttcgcccg tcagctcgct aagttgccca ttgcgcattt ccagcaggcg
    40081 gtcggcgtgg ataaagtaat gatcatcatg actgatagcg aaaatagttt tacccatctc
    40141 ctgcatcagc ggcagcaaca cctgataaaa ctcacgacgg aagtgtggat cctgatccac
    40201 cgcccattca tccagcagga taatatcgcg ttcttctgcc agcgccagca acagcgccac
    40261 gcgttttttc tgcccttttg ataacttcag gttaacaata cgcccgttgc ttaactcaag
    40321 cttatgagcc attttcagct gcgccagcca cttctcaacc agttgcgggt tagcgggttg
    40381 cccctccggc cccagcagtt gatcaaacag ccagacatcg gtaaacactg ccgaaaacag
    40441 tttgcgataa tcttccggtt gttcgccgct gacaggtttg ccatccagca agatttcgcc
    40501 gctttgtggc tgatacaagc ccgtcaacaa catcgccagc gtcgattttc cgctaccgtt
    40561 accgccaatc agaaacagca gctcgccacg tttgatggtg agattaatcg gaccaacgga
    40621 aaacgcgtta tcctgataag caaacgtcac gttacgcagc tccagcgttt gccagttggg
    40681 aaacgcctgc gggcgcggaa actcagcttt gaaaggcgcg agcgcgaatt tgttcagctt
    40741 gttaaacgcc acctgcgccg tcagcagcgt cggcaatgcg ccaaccgccg aaagcagcgg
    40801 cgtacgcagg aataacagcg tcaacgaata ggtcgcagca acgttggtat cagcccaacc
    40861 gagactgttc gccatccaga acaccaggcc gatagcgccc agcatcatga tgtttgacca
    40921 gttcacggca ctaagatgga aggtgtctgc gcgaataata tgatggcgat actcttgcgc
    40981 atcggggatg tagaggttgt taaacacata ctcggcgcgt tcccggttca gagtcagctc
    41041 tttgcgccct tcaagtacag tttgaaaatc cgtgtacagc ttgtcttcgg tttcacgtag
    41101 ggtcgccata tgtttgtaca cccgcgccac cagcacaaaa ccgccccaga tggtgatcgc
    41161 catccagata gccgttacca gcaacatttt gcccgacagc atccacagat acgccgatga
    41221 accgatagtg agaatgatcc cctgccccag ttccggcaga cgcacaaaag caatggtgat
    41281 attgcgcaca tcgctggtta atcccgccag caacgaggcg ctaccgagtt gttcaatgcg
    41341 ctcgacgtgg gtatccagaa tccgcttgat aaattcacta cgcagtcggt aaacgaagtg
    41401 atgccccaaa gtggtgagcg ccagttgcga tccgagagtg actgccatca acagcagcaa
    41461 taatcccaga aactccggta acaccagcag actggtatcc gccgtttcga taaggcgctg
    41521 attgataaaa gcaattaagc caatgcctaa tgccgcactg gcgaggctta gcgccatcac
    41581 actgataaat ggccagcgat actgccgcca gacaagtaca agaagttcca tgcagaaaac
    41641 ccggacaatg aattacagcc cgcagtttaa acatcttcgc gcgcacagca ataataattc
    41701 ttatttttat tcttttttac ctgcctgacg gaatgtcagg ttgtagcggc agtcggtggt
    41761 gagtggatga aaccccgctt tcaacggttg aataccgtga taaaacagcc gcgattcacc
    41821 gccccatacc accacatcgc catgttccaa caacaaacgt ttgagcggat cattacgttt
    41881 caggccacca aattgaaaaa tcgcgggtaa gcccagagaa acagaaacaa ttggcgcgcg
    41941 cagatccggt tcgtctttat cctgatgcag cgacagtttc gcgccaggag cgtagcggtt
    42001 gataagacaa gcatctggct ggaaatctgg atagcccgcc gccgtagccg cacgttgaca
    42061 taaatcatga aagctttgtg gcatagcggg ccacggttta tttgtttgcg gatcaatggg
    42121 cgaatagaga taaccttgcc gatgggtcgt ccagcccaga tgcccacagt tggtcatcgc
    42181 caccgacatg gtatatccgc ctggagcgac catctggcga aacggcgact ggctggcaac
    42241 gtcattaata tcgcggatta gttgctccgc agcattaaaa gcaaaacgcc gtaaaattac
    42301 cgcaccagcc gccagtggct cttgccacgg ttcagcatcg gcaaacagat ccaacattac
    42361 ctctcctcat tttcagcttc gcggcgcagc agttgcgctt tacgcgacac gccccagcgg
    42421 taaccggaaa gtgtgccatc accacggacc acccgatgac agggtattac gatagccagc
    42481 ttgttggcgg cacaggcgct ggcaacggcc cgtaccgctt tcggtttgcc gatggcgtta
    42541 gccagttgct gataactgac ggtttcacca caaggtatcg tgcgcagtgc ctgccagact
    42601 tgttgctgaa aagcagtgcc gcgaatatcc agcggtaacg tcagcggcgt atcgcgttga
    42661 ttgaggctgg cgatcacttc acgcacatgt tgctgaaacg tcagatcggc aggcgcgttg
    42721 tcggcagcgg gaaacatctg ctgcaactcg ctgattaatg tcgcgtcatc atcgcccagc
    42781 aatatcgcgc aaatcccccg ctcgctttct gccaccaggc aacgacccag ctcacaatca
    42841 gccagcgcgt aacgcaccgc cagattttcg ccaccgtgac ggaattgttt agccgtcatg
    42901 cccagcgttt catcggcttt gcggtagtaa ctgctgctgt cggggaatcc ggcgttaaga
    42961 atagacgtcg tcacgctctc ccctttcgcc agcgattcgc gcaaacggcg agcgcgccag
    43021 gcctgttgcc aggctttagg cgtcattccg gtagtagctt taaacaaccg atgtagatga
    43081 aatggactca tcgccacctg gtcggctaag gcttccagcg ttacaggcgt ttcctgttcc
    43141 agcagtcgac acgcgtgggt gattttatcc aaccgatgtt gccggggatt ggctttgtct
    43201 ggctgacaac gtttgcaggg gcgaaatccg gcggcgagtg cctcgctggc atttgcgtag
    43261 aaggagacat tttcccgcaa ggcatgtctg gcgcggcaag aaggacggca aaagataccc
    43321 gtggtgcgca cggcgaaaac gaattcgccg tcggcattcg ggtcgcgggc taagacagat
    43381 tgccagcgtt gatcgtcagt taagcatgtg gcgtttttca taatcagctc cctggttaag
    43441 gatagccttt agtctgcccg gtcaccatca cgcaaaaacc aacaatcttg cgctttaatt
    43501 tttttcgctg acaaggaagc ttttaaactg tggtgacatc caggttttaa agctatcgcc
    43561 ttctttggtg atcatataaa ccgccagccc ttcccggcga acaacctctt tggctttctc
    43621 cggcccgagt accatcaagc cagtatccca ggcatcggct tccagcgccg tcggagcaat
    43681 caccgtcacg gataccagat tgtgttcgat gggacgcccg gtttgcggat cgataacatg
    43741 ggaaagacgt ttgccgtcca gttcgtaata gttacggtag ctgccagagg tgctaatccc
    43801 atgaccgttg atatccacca cagcctgaac cgcgttttct ttatcggttg gtttttgaat
    43861 cgctacccgc cacggctggc cttcaccgtt cataccacgg ctgttcagcg cgccgcccac
    43921 cgacaccaga tagcgggaaa tcccttcctg ctccatcaag cgtgccaggt gatccgccgc
    43981 ataaccttca ccgacggtgg agagatcgac atataaatcc ggcaggtctt tttgcagata
    44041 ttgctgatgc gactgattga tgaccgtcag gtgctgtaag ccggttttgg ctttcatcgc
    44101 atcgatctgt tcctggctcg gaatttgaac cggctgttgt tccgggccaa agccccacag
    44161 attcaccagc ggcccgacgg taatatccat cgcaccatcg gtccttgccc caatgcgcag
    44221 cgaggtggtg acgatatcgg ccatcgcttc acttaccggc cacggcgaca aactttgcga
    44281 gtcgttaaag cgcatcagcg cggaatcttt tttataggtc gaaagcagct gatcgtcggc
    44341 gtccagctgg gtctgaatct tttctttaag ttcggcactg cgtttggcat caatgcccgg
    44401 gatgctggca cgccagaagg tacccatggt tttgccttca agaacggtaa cttcagtagc
    44461 gtgggttttg gcgggttgtg gtttttgatc gcaaccaaca aagaagagcg cggcaaccag
    44521 cagtgccacg cgggtaaagc ttatttccat acgtgattat cctcatgcga acggtcgcaa
    44581 gagtacacca aaaaactgtg tttgtacgct gaaaacaatg aaaaaagggc ccgcaggccc
    44641 tttgttcgat atccatcgag attagaactg gtaaaccaga cccagagcta cgatgttatc
    44701 agtgttgatg ccagcgtcac gagtgaactg gttgtcgtcc agcaggttga ttttgtagtc
    44761 aacgtaggtg gacatgtttt tgttgaagta gtaggtcgca ccaacatcaa catatttcag
    44821 gatatcttcg tcgtcgtagt tacgaccatt gatgacaccc aggtttttac ctttagactg
    44881 caggtaagcc agggacggac gcagaccgaa gtcgaactgg tactgagcaa cagcttcgaa
    44941 gttctgtgct ttgttcgccc aacccaagtt gcctacgcga gttgcgttgt aggtctgggt
    45001 gtactgagca gctaggtaga tgttgttcgc gtcgtatttc agaccaccgg tgtaggtttc
    45061 agcgcggtcg ccattaccga tgtaacgctc atcataacgg ttcagaccga agttctggtc
    45121 atcagtacgt ttggagctgg aaactgcagc accgataccg aagccttcgt aatcataagt
    45181 aatagaaccg ccaacgccgt caccgttctg acgcagagct tcacgaccgt tgttggtcat
    45241 gccttcacct tccgggctac cgtttttgcc ctgatactga acagcgaagt tcagaccgtc
    45301 aaccagacca aagaagtcgg tgctacggta ggttgcgaag ccgttaccac gctgctgcat
    45361 gaagttgtca gaaccgtaag tgtcaccacc gaattctggc agtacgtcgg tccaggaagt
    45421 tacgtcgtaa actacgccgt agttacgacc gtagtcgaaa gaacctacat cctggaattt
    45481 cagacctgcg aatgccacac gggtccagga gttgttttcg ttttcagcgc tgttgccctg
    45541 gatctgatat tcccactggc cgtaaccggt cagctggtca gtaacctgag tttcaccttt
    45601 gaagccaaga cgcatgtagg tctggtcgcc atctacagac ttgtcgtcag agaaatagtg
    45661 caggccgtct actttaccgt acagatctaa tttgttgccg tctttgttgt aaacttcagc
    45721 agcgtttgct gcgcctgcta ccagcagagc tgggaccagg agggacagta ctttaacttt
    45781 catgttatta accctctgtt atatgccttt atttgctttt ttatgccact gcatactgat
    45841 taaccctcat taatcagtcg gcaagtccat tctccccaaa aatgcagaat aatccaacac
    45901 gaatatgata ctaaaacttt taagatgttt catttatcgc tatagacgtt tcaaaatgta
    45961 aatacaaggg aactttttaa gattattgcg gaatggcgaa ataagcacct aacatcaagc
    46021 aataataatt caaggttaaa atcaataact tattcttaag tatttgacag cactgaatgt
    46081 caaaacaaaa ccttcactcg caactagaat aactcccgct atcatcatta actttattta
    46141 ttaccgtcat tcatttctga atgtctgttt acccctattt caaccggatg cctcgcattc
    46201 ggttttttta cccttcttta cacacttttc attattctgt gctaccacag aaaaactata
    46261 acgcttgtta actatttcac aaataattaa catcagcata atttccagca atctttgttt
    46321 atttgcaatt aatttcgttg ggctttttgt aggttatttg tacagcaaaa tggcgcttgt
    46381 acatctattt ccccccatac aggatgataa atatcacggg agaatagaga atcatcaatc
    46441 aggtaagagt ctggaatttc acactgtacc ctttatactg ccctatcact tcgcgaagtt
    46501 ttaacaggtc ataaacacga atgcgtcaga aagagacaac ggccacgacc cgcttttcac
    46561 tcctaccggg gagcattacc cgcttctttt tactgttgat cattgtgtta ctggtgacga
    46621 tgggggtaat ggtacaaagt gccgttaacg cttggctgaa agataaaagt taccagattg
    46681 tcgacattac ccacgcaatc caaaagcgcg tcgatacctg gcgttacgtg accaggcaga
    46741 tctacgacaa cattgccgcg acgacctccc cctcctccgg cgaaggttta caagagacgc
    46801 gcctgaaaca ggatgtctac tatctggaga aaccacgccg caaaacggaa gcgttaatct
    46861 ttggctctca cgacaactca acgcttgaga tgactcagcg gatgtccact tatctggata
    46921 cattgtgggg tgcagaaaat gtaccgtggt cgatgtatta cctgaatggt caggataaca
    46981 gtctggtgct gatctcaacc ctacccctta aagatctcac ctccggattt aaagaatcga
    47041 ccgtcagtga cattgttgat tcacgtcgtg cagaaatgtt acaacaggcc aacgccctcg
    47101 atgaacgcga aagtttttct aacatgcgcc gcctagcctg gcagaacggt cattacttta
    47161 ccttgcgtac cacgttcaac cagccgggac atctggcaac ggtcgtggct tttgatctgc
    47221 cgattaatga tttgatccca ccgggtatgc cgctggacag tttccgcctt gagccagacg
    47281 cgacggcaac gggaaacaat gataatgaga aagaagggac ggatagcgtc agtatccact
    47341 ttaacagtac gaagattgaa atctcctcgg cactcaactc taccgatatg cgcctggtct
    47401 ggcaggttcc ttatggcacc ttattgctgg atacgttgca aaacattctg ctgccactgc
    47461 tgctgaacat cggtttgctg gcgctggcgt tatttggcta taccacattc cgccatttct
    47521 ccagccgcag tacagaaagc gtccccagca cggcggtcaa taacgaattg cgcattttac
    47581 gggcaatcaa tgaagagata gtctcactgt tgccgctcgg cctgctggtt cacgatcagg
    47641 aatcgaaccg cactgtcata agtaacaaaa ttgccgatca tttgctgccg catctgaatc
    47701 tgcaaaacat caccaccatg gcggaacagc atcaggggat tattcaggcg acgatcaata
    47761 acgagctgta tgagatccgc atgttccgca gccaggtcgc gccgcgcaca caaattttca
    47821 ttattcgcga tcaggatcgc gaagtgctgg taaacaagaa actcaagcag gcgcagcgtc
    47881 tgtatgagaa aaaccagcag gggcggatga cctttatgaa aaacattggc gatgcgctga
    47941 aagaacccgc acagtccctg gcggagagtg cggctaaact caacgccccg gaaagcaaac
    48001 aactggcgaa tcaggcagat gtgctggtgc ggctggtcga agaaatacag ttagcgaaca
    48061 tgcttgcgga tgatagctgg aaaagtgaga cggtgctgtt ctccgtgcag gatttaattg
    48121 atgaagttgt gccttcagtg ttgcctgcca tcaagcgtaa aggtctgcaa ctgctgatta
    48181 acaatcatct gaaagcacac gatatgcgcc gcggcgatcg cgatgcctta cgacgtattt
    48241 tgctgctact gatgcaatat gccgtgacct caacgcaatt gggaaaaatc acccttgagg
    48301 ttgatcagga tgagtcctct gaagaccgcc tgacattccg cattctggac acgggagaag
    48361 gcgtaagtat tcatgaaatg gataatttgc acttcccgtt tatcaaccag acccaaaacg
    48421 atcgttatgg aaaggcggac ccgctggcat tctggctgag cgatcaactg gcacgtaaac
    48481 tgggcggtca tttaaacatc aaaacgcggg atgggcttgg tacacgctac tctgtgcata
    48541 tcaaaatgct cgccgctgac ccggaagttg aagaggaaga agagcgttta ctggatgatg
    48601 tctgcgtaat ggtggatgtt acttcggcag aaattcggaa tattgtcact cgcctgttag
    48661 aaaattgggg tgcaacctgt atcacacccg atgaaagatt aattagtcaa gattatgata
    48721 tctttttaac ggataatccg tctaatctta ctgcctctgg cttgctttta agcgatgatg
    48781 agtctggcgt acgagaaatt gggcctggtc aattgtgcgt caacttcaat atgagcaacg
    48841 ctatgcagga agcggtctta caattaattg aagtgcaact ggcacaggaa gaggtgacag
    48901 aatcgcctct gggcggagat gaaaatgcgc aactccatgc cagcggctat tatgcgctct
    48961 ttgtagacac agtaccggat gatgttaaga ggctgtatac tgaagcagca accagtgact
    49021 ttgctgcgtt agcccaaacg gctcatcgtc ttaaaggcgt atttgccatg ctaaatctgg
    49081 tacccggcaa gcagttatgt gaaacgctgg aacatctgat tcgtgagaag gatgttccag
    49141 gaatagaaaa atacatcagc gacattgaca gttatgtcaa gagcttgctg tagcaaggta
    49201 gcctattaca tgaacaatat gaacgtaatt attgccgatg accatccgat agtcttgttc
    49261 ggtattcgca aatcacttga gcaaattgag tgggtgaatg ttgtcggcga atttgaagac
    49321 tctacagcac tgatcaacaa cctgccgaaa ctggatgcgc atgtgttgat taccgatctc
    49381 tccatgcctg gcgataagta cggcgatggc attaccttaa tcaagtacat caagcgccat
    49441 ttcccaagcc tgtcgatcat tgttctgact atgaacaaca acccggcgat tcttagtgcg
    49501 gtattggatc tggatatcga agggatcgtg ctgaaacaag gtgcaccgac cgatctgccg
    49561 aaagctctcg ccgcgctgca gaaagggaag aaatttaccc cggaaagcgt ttctcgcctg
    49621 ttggaaaaaa tcagtgctgg tggttacggt gacaagcgtc tctcgccaaa agagagtgaa
    49681 gttctgcgcc tgtttgcgga aggcttccta gtgaccgaga tcgctaaaaa gctgaaccgc
    49741 agtattaaaa ccatcagtag ccagaagaaa tctgcgatga tgaagctggg cgtcgagaac
    49801 gatatcgccc tgctgaatta tctctcttct gtgaccttaa gtccggcaga taaagactaa
    49861 tcatctgtag gccagataag acgcgttagt gtcttatctg gcatttgcac cgattgccgg
    49921 atgcggcgta aacgccttat ccggcctacg attcccatta tttcaacaga ttacattaaa
    49981 gtaggccaga taagacgcgt cagtgtctta tctggcattt gcactgaatg ccggatgcgg
    50041 cgtaaacgcc ttatccgtcc tacgaatccc gcgatttcct gaccctctcg gcatataccg
    50101 tcagcgtctg ttttatcaca tccagcgtta ccggcttcga gaggcagctg tccataccgg
    50161 actccagaca ccgctgcttc tcttcagcca acgcattagc agttactccg attaccggca
    50221 acgtcagtcc caactgacga atgcgttgcg tcaagcggta accatccata tttggcatgt
    50281 tgacgtcgct aagcacgata tcaatatgat tcttactaag tacattaagc gcatcgacgc
    50341 catcattcgc ggttttacat tgatagccca acgatcccaa ctgatctgcc agcagacgtc
    50401 ggttaatcgg atgatcatcc acgaccagaa tcatcatatc gtcgttatcg ctgaccgctt
    50461 tgtccgtcga cggcagagcg ttagcaggat cgtcgctctc catctcgatc aaataaatac
    50521 gcgccaacaa tgccggtagc tcatgcggag cagccacact gtgtacccac tcccctggcg
    50581 ctttctccag cggaatacca atatggcgac gacagaaggt cactaccgct ctgccctgcc
    50641 attttttact cactacctcg tcagtgatta acacatcttc gggagtcggt tcctgccctt
    50701 cgtatgttgt aacgacgatg ccgctgcgct gcaaactggt ttccaggaac tgacagagcg
    50761 acgcattgcg gaccgccagc cagcagcgtt taccactcaa cccttccacg ccttttttct
    50821 gcgggtactg agcgccgtac aacggaatac gcacggtaaa ctggctgccc attcccggtt
    50881 ctgaatctac cgagatatcg ccgtccatca tgctgatcag tttttcacaa atcgccagac
    50941 ccagaccggt cccctggaag tttcgctgta cgcccgttcc aacctggaag aagggatcaa
    51001 acaagcgcac cacttctttc gccggaatcc ccacgccggt atcgcgaaca cggatagaga
    51061 gataatcgcc atcagcgcga acatgcaaaa ctatacagcc ggtatcggtg aattttatgg
    51121 cgttactcaa caggttggag atgacctgct gtaaacgcat cgggtcgcca tttaaggcca
    51181 ctggcacatc cggttcaata aagcagtaca agcctaactg cttgcgtacc accagcggta
    51241 aatagttggc ggtgatgtgg ttcatcactt cacgcggtga aaactcacgc ggttcgatct
    51301 tcaactgttc cgattcaatc ttcgagaaat cgagaatatc gctgataatt ttcaacaaca
    51361 ggctggaaga gttgttcatt gccgtcacca gacgatcgac gcctttcggt aactctttgg
    51421 tttgcaacag atccaggtta ccgataatgc catacagcgg cgttcgcagc tcatgactga
    51481 cggtggcaag gaacatcgat tttgactggc tcgcctgttc cgctgcttgt gccatctcct
    51541 gcaacgactc ttccatcttc acgcgcgaag aaacatccac cagcacacaa atggccacgt
    51601 tttcattacg atagcgcgaa tggacgaagc tgatttgcag attggtattg ttgctggtca
    51661 ggacatcaac aaaattgacc tgctgcccac agataatttg cgtcagtcgt tggcggtcct
    51721 catgcgtaag catattgaga taggtatgcg ccagttcgtt acttaaaata ttgacgccat
    51781 cagcggtacg caaaatgcag atacccactg gcgcggaggc gacaatcttg cgattgaact
    51841 gctcatgttc ttccagtcgc agggcgtcgc tttccgccgg aatgaaaata cgtcgctcgt
    51901 acatccgcgc gagagtaaac aatgcagctc cggcaagcac attcagcaaa attgcgttaa
    51961 ggatcaacat gcgaatgcgt tccagcacct tatcaaccgg caccgaatac acgatgctta
    52021 gcgatgaggg tggcagattt ttcttcagca ccagctcccg gaacccttcc gtatagccaa
    52081 accaggagcg ttcctgcatc cagcgaggat cgcccttaat tttactttct ggtccggtaa
    52141 gcgaaatcag ggtatgacca ttttcatcaa gaatggtaac ccccatcggc aacgtacccg
    52201 gtaagaaaaa gttctccatc cggatggtct gctcgacacc caaaagcgcc tgcaaccggt
    52261 tcgccagata aactggcgtc aacgcgtaaa aatacccgac gcaagggcgc ggaccttcgc
    52321 tgatccagta gaggttactg ccgctatcat cttgtggtgc atttcgatat ttattgatgc
    52381 gttcatgcaa cgctttcaac gcggtatcgc gttccactgg catatcacgc agaccgaaat
    52441 tggccatgca gaggttatca ctgccgatta aaaatacccg gttgagatcg taagccgcag
    52501 aaaaattatc gcgccagtag cgcataaacc acgccaatga ctccagagaa cctcgccagg
    52561 tgttactcat tgcggaacaa tcggagtcgg caaacagcgg ttcaaacgca ggcacatccg
    52621 cctgcgtttc tcgtccacgc ggggaaagca caccgttttc tgccgataag cgattttcgg
    52681 cgatgtactt aagctctttc atcacatcag aggtgcgttg aataaagcgc tgagcctgat
    52741 cggaactcag attaaattcc tgacgaattt ccgattctcg ctgatgtaac gcattaacga
    52801 tgtaaaaaac ggatgaaaaa gcaatcaaca gccagagcac taacgccaat gctctgaaca
    52861 tgtagcgcga ggctttcagg gttgtacgaa aagaagcaag gtatttcaaa ggggagaagc
    52921 tccgcctcag gtgaccgatg gagtgtggtt aaggtagcgg taaaagcgtg ttaccgcaat
    52981 gttctgtcac cattgcaata atgcgtatcg cttagaaacc attgccttaa ccaggaaagc
    53041 cggagtagat ggacagttct cagtacgaaa aataagaaat gaccaatctg ccgacgcttg
    53101 ttaatggtta tatcctggcg tcggcagatc gcccagcagt cttatctgaa aaaagagaga
    53161 gccaggttgt gtgaagggat ggatcaccag tcaaaacttt tcaccagcgt cagctcgcca
    53221 gcattacgca tcggtacaat aaatgtttcc tgtttctcat tgaccgatcc ttcatcggtg
    53281 atcagcgtca gttgggcggt cgtcaattcc gtttcgctgc gcccaccata gtagttgata
    53341 tacacctgat agcgcccgtg aactggtgct ggcatggcga aaatctcggg tccgtacccc
    53401 gtcgtgacat ccatatccag tgcaccactg tttttcagca cggtgttacc gtaccaggcg
    53461 tgttcgccat cgggcgtgac aacatggaga tcgagatcgg tattgtccgt atcccacgag
    53521 agaaccagcc gtaaacgtgc acgaatagtt cctgtacccg gcgttgagta aaactgcatt
    53581 ttttgccggc tttgcccatc cgggctgatg acctgcacgc tgttgctgcc ttcagtgaaa
    53641 atataagggc gtgcaaatga accatcggat tcaattctct gcggcatact ggcaccgttg
    53701 accaccagcc ggccctgctg aactttaccc gccgcagcat aattctttat tttgccgcgg
    53761 atctgcgctg agatattttg atcatctgcc atgttgacgg acgacgccgg gtaattgatc
    53821 gattgtgaaa aactggcatc ttcgccctct gccggatgcc agccagagag cggtgcgtca
    53881 atctcgactt cctgcacacc ttcactgtga gcgacaggcg aaagtgccac cagtaacaac
    53941 ggaagaaaaa tctttcgcat agcattagtc cattaataat tgtcgggtca gcgtttcgat
    54001 atagttctca tcaagcccgg tgggataacc atcaaaggcc aagtgcagat actcatgggt
    54061 taaatccagc cgatcctgaa gcgtaaagaa gttacgaata aacagccgtt tctgctgccg
    54121 atcggtatag gggaaacctg agactaaacg acagaccgca aacacgtctg gttcgttgta
    54181 ccccgtctcg ccttgtaata tacgccgcca ctgcggcatt tttttcgcca gccaggcttt
    54241 cgctttgggt aataactggc aggttgagcg tggagctccc cagcggctaa ggctgttgtc
    54301 gggataagca aacgcgagta tctggtcgta acgctccccc tgccccgcct gggccgtggc
    54361 ttgccgccag gaaagtgtcc cttcggtagc gcgactgcca tgataatgaa ccggatcgcc
    54421 tgcatagatg agctcctgcg tccaggcggt catcgttcgc gcccccgtgg tcgctggcga
    54481 agcagaaaca cgctgcgtgg cgctgctgtc ggggatggtc aggcaatcac cttcccgatt
    54541 ggcgttttgc tgtaaaaaag tacgtatcgc cacagtcatg gctttcgctg cctcaggtgg
    54601 cgtacttttc gcctcgcgat ccagaactct ggcaacgtac tcttcacgat cgagatgcga
    54661 ctgcaatttc agtttgccct tttcgctcag taaggtcgtc tcaccgtggc tgacaaacgt
    54721 aatgtggtta ccattagtaa acgtcacacg atagcgccca ttaagtacgc tgggttttac
    54781 tgccgtcgta ctcttttccg ctgtgatttt cttcagcgga tagcgggcaa acagctccac
    54841 ctctacacat tgcccactgg caacctgcgt cggcactggc aaaacccgat taagcaccgt
    54901 cgcataacgg gttagtaccg ttttgctggt gccactgcca gtaacccaca gcggtgtacc
    54961 atcggttaac cagccagcaa aaccaccctg acggatttcc tgtttatcat cggcaaacca
    55021 gctccaggtt ttcacccgta cccgactacc gagcatggat gcgacaccga ttttcgcctc
    55081 gtccagcacc acatccagta agacttcctg agctttgttt tgcgcaggta acgtcgctaa
    55141 tgaatcaagc agcgatttaa ccgttaccga ggtttctggc ttcagcgttg taagagacgc
    55201 taaccacgca ggtgcctgac gctgttgcca gtattgcccc cacacatctg cgccaatatg
    55261 caaccgctgc ggcgcaaaat agagcccaca ggaacgtacc agcgcggtat cgcgcgttat
    55321 gctctcccca gcctgacaac aataaacctc ttccggggag tttccgcgac actggtaggg
    55381 ctgctccggt tgatgggtat cctccagcca ggcgtaaaca tacagtttcc acaaactccc
    55441 taacggcgtg gtgagcgagt caggtaatgc tgagactttg gtcacgcctg atgacgacag
    55501 ctgatacagt tgatcgtgct gcgcaccgcg cagcaccagt tgtaacggcg tctcctccgc
    55561 aagcgttggc agagtaacca acgccaatag ccagacgatt ctgcgccagt tcacttattt
    55621 cacctgcatc ccggtccatt cactccctgc cgcgacactt tgctgcgcag gtgcatagga
    55681 acgcacataa cgagcaggag gcagaacgaa ttgccctttt tgcgagaagc gcagcaaatg
    55741 gcggaaagtg accgttcccg tcagttcttt caccggcacc atataggcca gttcgcccat
    55801 ttcattacgc gctttttcca gcaattgccc ctgctgtttc gcggcgttgg gtttattgac
    55861 tgaaatgccc catgttgtgc gctcaacgtc agctcccggc gggagcggta cttccacctg
    55921 accatagcgc agaactgcat cctgctcgct ggtaagcgta atttcatcga gatacagcgc
    55981 atcgctgtca atctcattgc tggtcaccgg ctgcagagta aagctcatct cttcttcacc
    56041 ggggataagc cgatacaact ggcgttcaac ggtcaccgga atgttacttt gttgagccgt
    56101 ttttgccggt tcacgccagc ggacctgcac atttgcggcg ataattcgtc accaaaagag
    56161 agaatgtccg gcacacccta accaacccaa cgccagtctt cgccccctcc agttaactta
    56221 tgtttagccc atgcgcccgc aggcgcaggc aaaacaactg gaggcattgt cgccatatat
    56281 ttcgccagcc agttcatggc cagcgcgcgc tcaatagtgg attgctcagc ggtcaaacca
    56341 ttaaaatagc ggccgtctgg gtagcgtcac caccgctatt gagcagcaca accgtgtgcg
    56401 ccagcggctg attgctgttt gccgctttat cccacgcata ttgctgaacc tgtggcatca
    56461 ccgtggactt caactgcgct tttttcagca acgtcatggt taagacgttt gccaccgcat
    56521 ctgccagtgg agattccggt gtatcgagga tcaggctgtc attgatatcg cggatgtctt
    56581 cctcagagaa atcttcatct ttagttccgc gccgggcgat agcttcatcc ccccctttca
    56641 acaacgtttt gcacggcaga ttcatctcct gtgcccacgc cagcaccagc gcccgatgca
    56701 ataacggcat attatccgcc tgctcggcat aactgtcgag catatgctgc cagtattccg
    56761 gttgttgcgt tacgccgagc gcctggctgg cctgccagtc ggcgtaccat gcccatgccg
    56821 taaggaaggc gtcaccattg ccatcttcac cccaccaggt aaagcgcgct ccgggccccg
    56881 ccagttgcat cagccgcaga cggttatcct gaatcatctg acgaatgtcg ttagcggcgg
    56941 cactttgatg atcggcaagc gaacgccagg cgagactgag cgggatcaga cggctaccgg
    57001 tgttgatcac cccaccccac ggttcatcga ctaacgcatc aagattgttg cggaaaatct
    57061 cctgcggcgt ttcactactt tgtagccgga tattactcgc ctgctcgggc aacatcagcg
    57121 cgttctcgcc accgccgagc atgacatttt tctgctgttc aacgggccag ctgttgtcca
    57181 caaaagacag ttttgtgcta atgctgtcct gcacttgccc attttgttgc agttctgcac
    57241 ttaacaagcc agattgctga atattctgcg tcagcgaaat ataattcgcc cctttgtgca
    57301 gcgtcagcgt ctggcgcatc tcagcgcctg caaatttagt caccagcgcc accggttcgt
    57361 tatcctgctg actgaagata aacagtcctg ccgccggttt gtcgcccacg cgatacaccg
    57421 ttggcatact ccacttcatg tagagatttt tttccgaacg cagataagca cgcccctgcc
    57481 cgaccagccc gtcgccgttc atcccacgcg cggtgatacg ccagcgggtt aacgaatcag
    57541 gcatcaggaa cgtgaaatac gctttgcctt gtttatcggt tgtgagtgac ggcatccatg
    57601 ccgcggtatc cacctcttca cgccgtggac gttcaagcat ttttactcgc cgctcgctgc
    57661 ggttagtcgc gccaggcgca accggctcgc tggagagcgc ctggtcatag ctgataaacg
    57721 acagactgga gctggtacgc acattgttac gccccagcgg atagaaaaat ttgccgatat
    57781 tcggcgcgat ttctggttgc agcgcgtaga tcatttcatc gaccacgcct accgttagct
    57841 gcgcagaaac aggtttacct ttcagcgacg aggtta
//
LOCUS       DAJTUI010000016        55410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTUI010000016 DAJTUI010000000
VERSION     DAJTUI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.016"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(104..1477)
                     /gene="argH"
                     /locus_tag="OO464_001049"
     CDS             complement(104..1477)
                     /gene="argH"
                     /locus_tag="OO464_001049"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6226284.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1538..2314)
                     /gene="argB"
                     /locus_tag="OO464_001050"
     CDS             complement(1538..2314)
                     /gene="argB"
                     /locus_tag="OO464_001050"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6226285.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2322..3326)
                     /gene="argC"
                     /locus_tag="OO464_001051"
     CDS             complement(2322..3326)
                     /gene="argC"
                     /locus_tag="OO464_001051"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6226286.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3480..4631
                     /gene="argE"
                     /locus_tag="OO464_001052"
     CDS             3480..4631
                     /gene="argE"
                     /locus_tag="OO464_001052"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6226287.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4983..7634
                     /gene="ppc"
                     /locus_tag="OO464_001053"
     CDS             4983..7634
                     /gene="ppc"
                     /locus_tag="OO464_001053"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6226288.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7817..9550
                     /locus_tag="OO464_001054"
     CDS             7817..9550
                     /locus_tag="OO464_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6226289.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9699..10550
                     /locus_tag="OO464_001055"
     CDS             9699..10550
                     /locus_tag="OO464_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6226290.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10537..10878)
                     /locus_tag="OO464_001056"
     CDS             complement(10537..10878)
                     /locus_tag="OO464_001056"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6226291.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10880..11758)
                     /locus_tag="OO464_001057"
     CDS             complement(10880..11758)
                     /locus_tag="OO464_001057"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6226292.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLTPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11724..14021)
                     /locus_tag="OO464_001058"
     CDS             complement(11724..14021)
                     /locus_tag="OO464_001058"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6226293.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14072..14392)
                     /locus_tag="OO464_001059"
     CDS             complement(14072..14392)
                     /locus_tag="OO464_001059"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6226294.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14407..15486)
                     /locus_tag="OO464_001060"
     CDS             complement(14407..15486)
                     /locus_tag="OO464_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6226295.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15795..16898
                     /locus_tag="OO464_001061"
     CDS             15795..16898
                     /locus_tag="OO464_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR6226296.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16899..18296
                     /gene="ptsP"
                     /locus_tag="OO464_001062"
     CDS             16899..18296
                     /gene="ptsP"
                     /locus_tag="OO464_001062"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6226297.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18308..18970
                     /gene="fsa"
                     /locus_tag="OO464_001063"
                     /pseudo
     CDS             18308..18970
                     /gene="fsa"
                     /locus_tag="OO464_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18981..20082
                     /gene="gldA"
                     /locus_tag="OO464_001064"
                     /pseudo
     CDS             18981..20082
                     /gene="gldA"
                     /locus_tag="OO464_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20357..20964
                     /locus_tag="OO464_001065"
                     /pseudo
     CDS             20357..20964
                     /locus_tag="OO464_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(20991..21896)
                     /gene="yijE"
                     /locus_tag="OO464_001066"
     CDS             complement(20991..21896)
                     /gene="yijE"
                     /locus_tag="OO464_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6226298.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21990..24170)
                     /gene="katG"
                     /locus_tag="OO464_001067"
     CDS             complement(21990..24170)
                     /gene="katG"
                     /locus_tag="OO464_001067"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6226299.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24499..25389)
                     /gene="metF"
                     /locus_tag="OO464_001068"
     CDS             complement(24499..25389)
                     /gene="metF"
                     /locus_tag="OO464_001068"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6226300.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25738..28170)
                     /gene="metL"
                     /locus_tag="OO464_001069"
     CDS             complement(25738..28170)
                     /gene="metL"
                     /locus_tag="OO464_001069"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6226301.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28173..29333)
                     /gene="metB"
                     /locus_tag="OO464_001070"
     CDS             complement(28173..29333)
                     /gene="metB"
                     /locus_tag="OO464_001070"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6226302.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29610..29927
                     /gene="metJ"
                     /locus_tag="OO464_001071"
     CDS             29610..29927
                     /gene="metJ"
                     /locus_tag="OO464_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6226303.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30111..30719
                     /locus_tag="OO464_001072"
     CDS             30111..30719
                     /locus_tag="OO464_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR6226304.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30780..30992)
                     /gene="rpmE"
                     /locus_tag="OO464_001073"
     CDS             complement(30780..30992)
                     /gene="rpmE"
                     /locus_tag="OO464_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6226305.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31195..33393
                     /gene="priA"
                     /locus_tag="OO464_001074"
     CDS             31195..33393
                     /gene="priA"
                     /locus_tag="OO464_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6226306.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33549..34574
                     /gene="cytR"
                     /locus_tag="OO464_001075"
     CDS             33549..34574
                     /gene="cytR"
                     /locus_tag="OO464_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6226307.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34666..35625
                     /gene="ftsN"
                     /locus_tag="OO464_001076"
     CDS             34666..35625
                     /gene="ftsN"
                     /locus_tag="OO464_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6226308.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35718..36248
                     /gene="hslV"
                     /locus_tag="OO464_001077"
     CDS             35718..36248
                     /gene="hslV"
                     /locus_tag="OO464_001077"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6226309.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36258..37589
                     /gene="hslU"
                     /locus_tag="OO464_001078"
     CDS             36258..37589
                     /gene="hslU"
                     /locus_tag="OO464_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6226310.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37656..38582
                     /gene="menA"
                     /locus_tag="OO464_001079"
     CDS             37656..38582
                     /gene="menA"
                     /locus_tag="OO464_001079"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6226311.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38675..39160
                     /gene="rraA"
                     /locus_tag="OO464_001080"
     CDS             38675..39160
                     /gene="rraA"
                     /locus_tag="OO464_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6226312.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39245..39490)
                     /gene="zapB"
                     /locus_tag="OO464_001081"
     CDS             complement(39245..39490)
                     /gene="zapB"
                     /locus_tag="OO464_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6226313.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39916..40761
                     /gene="glpF"
                     /locus_tag="OO464_001082"
                     /pseudo
     CDS             39916..40761
                     /gene="glpF"
                     /locus_tag="OO464_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40801..42291
                     /gene="glpK"
                     /locus_tag="OO464_001083"
     CDS             40801..42291
                     /gene="glpK"
                     /locus_tag="OO464_001083"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR6226314.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42521..43531
                     /gene="glpX"
                     /locus_tag="OO464_001084"
     CDS             42521..43531
                     /gene="glpX"
                     /locus_tag="OO464_001084"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6226315.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43628..44374
                     /gene="fpr"
                     /locus_tag="OO464_001085"
     CDS             43628..44374
                     /gene="fpr"
                     /locus_tag="OO464_001085"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6226316.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44379..44807)
                     /gene="uspD"
                     /locus_tag="OO464_001086"
     CDS             complement(44379..44807)
                     /gene="uspD"
                     /locus_tag="OO464_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6226317.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44834..45133)
                     /locus_tag="OO464_001087"
     CDS             complement(44834..45133)
                     /locus_tag="OO464_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6226318.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45345..45785)
                     /locus_tag="OO464_001088"
                     /pseudo
     CDS             complement(45345..45785)
                     /locus_tag="OO464_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45886..46485
                     /locus_tag="OO464_001089"
     CDS             45886..46485
                     /locus_tag="OO464_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6226319.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46593..47360
                     /gene="tpiA"
                     /locus_tag="OO464_001090"
     CDS             46593..47360
                     /gene="tpiA"
                     /locus_tag="OO464_001090"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6226320.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47415..48170)
                     /gene="cdh"
                     /locus_tag="OO464_001091"
     CDS             complement(47415..48170)
                     /gene="cdh"
                     /locus_tag="OO464_001091"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6226321.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48277..49266)
                     /gene="sbp"
                     /locus_tag="OO464_001092"
     CDS             complement(48277..49266)
                     /gene="sbp"
                     /locus_tag="OO464_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6226322.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49586..50548)
                     /gene="pfkA"
                     /locus_tag="OO464_001093"
     CDS             complement(49586..50548)
                     /gene="pfkA"
                     /locus_tag="OO464_001093"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6226323.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50729..51631)
                     /gene="fieF"
                     /locus_tag="OO464_001094"
     CDS             complement(50729..51631)
                     /gene="fieF"
                     /locus_tag="OO464_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6226324.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51779..52279)
                     /gene="cpxP"
                     /locus_tag="OO464_001095"
     CDS             complement(51779..52279)
                     /gene="cpxP"
                     /locus_tag="OO464_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6226325.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52429..53127
                     /gene="cpxR"
                     /locus_tag="OO464_001096"
     CDS             52429..53127
                     /gene="cpxR"
                     /locus_tag="OO464_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6226326.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53124..54497
                     /gene="cpxA"
                     /locus_tag="OO464_001097"
     CDS             53124..54497
                     /gene="cpxA"
                     /locus_tag="OO464_001097"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6226327.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54603..55277)
                     /gene="yiiM"
                     /locus_tag="OO464_001098"
     CDS             complement(54603..55277)
                     /gene="yiiM"
                     /locus_tag="OO464_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6226328.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNAHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13488 a        15016 c        14164 g        12742 t
ORIGIN      
        1 caaatttctg cctgatgcgg cgtaaacgcc ttatccgcct acgaagtgtc gcactttgta
       61 ggcgtggtaa actccccatt ttcaggggag caatataaac aaattactct aaccgagcct
      121 gcgcaaaagc aatcgcctgc gccacctgct gcggtgagac gccgcctttt gccgcacgct
      181 tttcgaggca cgattgtagc gacagaatcg gatagacatc ttcgccaatc acctgactga
      241 atttctgcaa ctcactgagt ggcagatctt ccagcggttt gccctgacga atggcttcca
      301 ccaccgcttc acccacaata tggtgcgcct cgcggaaagg tacgcctttc gccaccaggt
      361 aatccgccag ttcagtggcg ttcgcatacc cctgctgcgc cgcttcctga caacgcggac
      421 gtttcacctg aatgccgtcc agtaccagcg ccgccatatg caggcagtcc agccaggtat
      481 cgagcgcgtc gaacagacct tctttgtctt cctgcatatc tttgttgtaa gccagcggca
      541 aacctttcag cgtcatcatc atgccggtta acgcaccctg cacccggccg catttaccgc
      601 gaatcagctc cagcgcatcc gggtttttct tctgcggcat taatgatgaa ccggaagtca
      661 cgcggtccga aagctccaca aaccccgctt cgccggtgtt aaagaaaatc agatcttcag
      721 caaaacgcga cagatgcacc atgccgatag cggcagcaga aagcagttcc aacacatggt
      781 cacggtcaga aacgctgtcc agactgttac gggtcgccga agcaaagccc agccagcctg
      841 ctaactgttc acggtcgatt tcataggccg ttcccgccag cgcgccacag cctaatgggc
      901 tgacatccag acgcttaagc gcatcctgca aacggctttc atcacgcgcc agcatctcaa
      961 cataggccag gcaccagtgc gcgaacgtca ccggctgggc gcgttgcagg tgagtgtaac
     1021 ctggcattac cgcgtcctga ttgttttgtg cggtttccac cagcgccgat tgcagctgcc
     1081 ggttagccgt cagtaactcg ctaacggtat ctttgcacca cagtttcagg tcagtcgcta
     1141 cctgatcatt acggctacgc ccggtatgca gctttttgcc taactggccc actttgtcga
     1201 tcagtttgcc ttccacccag ctatggatat cttcggcgtc gctttcaagg atttgttgtg
     1261 gcctggcgcg aacatcttcc agcaacacgt tcagcgcctc ttccagttgc gcctgctctt
     1321 ctgcggttaa cacgccgacc gttaccaagg ctttggacca ggccacagag ccaacaatat
     1381 cctgctccgc cagacggtaa tcaaagcgca gtgagtcgtt gaattgtttg aaccgttgat
     1441 ctgctgcctg ggtaaaacgc ccgccccaaa gtgccataac tctgtttcct tattttgaaa
     1501 ttcaatgccg gaaagctgcg cctccggcta acaaaactta agctaaaatc cgcgtaccca
     1561 tcggcatacc gttaaacagt gccggaagct gctccgcata acgccaagaa gcgatatcta
     1621 ccggacggcc cagcgtgcgg gccgcatcca gcgccgcgtt cactttcact atcatgccgt
     1681 cagtaataat gccctgctca atcagttgtt ctgctttcgc ggcggtcatt tcggcaatgc
     1741 gttgcccttt gccgtcgaga atgccgctga cgtcggagag caaaatcaga tccgcgccca
     1801 gcgttgccgc cagcgccgtt gccgcctggt cggcattgac gttcatcagt tgcccttcgt
     1861 ctgttacgcc aatggagctg accaccggca gataaccgtt ctccagcaag gagttgataa
     1921 gcttaggcga acctggctgc gccagtccaa catgacctaa ctcttcatca agctgggtca
     1981 ctttgacgct gtcgccgtca ccgagaaaca aacctacggc cgcaatctga tgtttcttcg
     2041 cccatgccaa cagggtttta tttgccgttc ccgccagtgc tccggtgata atgtctatct
     2101 gatcagcagg cgtcacccgc aggccgtttt tctttttcac cggcagattc agccctttca
     2161 tcagctcatc caccacgcaa ccgccgccgt gcacaatcac cagcggacgc tgatgtgact
     2221 cacgataatt caccaatgcg ctaaacagac gttccagcgc ctcttcacta tccagcagta
     2281 cgccgcccag tttgataatt aatggattca tcattgcacc cttaaataag agactgcgtt
     2341 tccgcatagc cgaaacgaat attggcgcac tgtaccgctt gtgccgccgc gcctttcagt
     2401 aagttgtctt cggtcgccac aataatcaga tgctcgccct gaacggcaaa cccgatatcg
     2461 caaaatggca gcccaacgac atttttcagc gccggaacgc ctttgtcata cagccgcacc
     2521 agcggtttat gggcatacgc ctgctgtaac gcttgcgcga cttgcgcctg ggtcacaccc
     2581 gatttcaggc ggcaggtaat ggtttcgaga atgccgcgcg ggaaattgcc cagatgtggg
     2641 gtgaagataa cgtcagcacc gaggtgtgtg gcgatctctg gttgatggcg atgagtaaag
     2701 acgccatacg gttgcaggct aacttcacaa aaactatttg aaatcgctgc cttacgtcct
     2761 gcaccgctca cgccgctggt ggcgttgatc accggccact gattgaggtc aagaagatcg
     2821 gcatcaatca acggtttcag cgccagctgt gccgccgtcg gataacagcc tggcaccgca
     2881 atcaaattcg cttcttttaa tttattaccg caccactccg ccagaccgta ggctgcctgt
     2941 tccagcagtt ccgggtattg atgggtaaag ccgtaatatt tttcatagaa ggtggcgtcg
     3001 ttaacacgaa acgcgccgga aaggtcgaac accacgcagc ccgcttcaag aaactgcggc
     3061 gctaaatcgt ggctaacttc atgggcggtg gcgagaaaca ctacgtccac ccctgggcta
     3121 aactcgctga tatccgacat cggctgcaac ggcagctcaa cgatgccttt tagctgcgga
     3181 tgcaaatcgg agattaactt tcccgcatca ttgctttgcg ctgaaacagt caaagcggtt
     3241 atgttcatat gcggatggcg atttacatag gtcactagct ctgcgccagc gtagccgctg
     3301 gcacccacaa tcagcgtatt caacatcggg gctattcacc ttcttatgtc tggttgccag
     3361 gttaaacgta aaacattcac cttacggctg gtgggtttta ttacgctcaa cgttagtgta
     3421 tttttattca taaatactgc atgaatattg atactatcat gaccagaggt gtgtcaacaa
     3481 tgaaaaacaa attaccgcca tttatcgaga tttaccgcgc tctgattgcc acaccttcaa
     3541 taagcgctac ggaagaggca ctcgatcaaa gcaatgcaga tttaatcact ctgctggcgg
     3601 actggtttaa agatttgggc ttcaatgtgg aagtgcagcc tgttccagga actcgcaaca
     3661 aatttaatat gctggccagt tacggacagg gggctggcgg cttgttgctg gcggggcata
     3721 ccgatacggt gccatttgat gacggtcgct ggacgcgcga tccgtttaca ctgacggagc
     3781 atgacggcaa gctttacggc ttaggcaccg ccgacatgaa aggctttttt gcgtttatcc
     3841 ttgatgcgct acgcgatgtc gacgtcacga aactgaaaaa accgctctac attctggcga
     3901 ctgctgatga agaaaccagt atggccggag cgcgttattt tgccgaaact accgccctgc
     3961 gcccggattg cgccatcatt ggcgaaccga cgtcactaca accggtacgc gcacataaag
     4021 gtcatatctc taacgccatc cgtattcagg gccagtcggg gcactccagc gatccagcac
     4081 gcggaattaa cgctatcgaa ctaatgcacg acgccatcgg gcatattttg caattgcgcg
     4141 ataacctgaa agaacgttat cactacgaag catttaccgt gccataccct acgctcaacc
     4201 tcgggcatat tcacggtggc gacgcttcta accgtatttg cgcttgctgt gagttgcata
     4261 tggatattcg tccgcagcct ggcatgacac tcaatgaact taatggtttg ctcaacgatg
     4321 cattggctcc ggtgagcgaa cgctggccgg gtcgtctgac ggtcgacgag ctgcatccgc
     4381 cgatccctgg ctatgaatgc ccaccgaatc atcaactggt tgaagtggtt gagaaattgc
     4441 tcggagcaaa aaccgaagtg gtgaattact gtaccgaagc gccgtttatt caaacgttat
     4501 gcccgacgct ggtgctcggt ccgggttcca tcaatcaagc tcatcagcct gatgaatatc
     4561 tggagacgcg gtttattaaa cccacccgtg agttgattat ccaggtaatt caccattttt
     4621 gctggcatta aaacgtaggc cggataaggc gctcgcgccg catccggcac cgttgccaaa
     4681 ctccggtgcc gcaataatgt cggatgcgat gcttgcgcat cttatccgac ctacaccttt
     4741 ggtgttatgt atggcgattt tttaacattt ccataagtta cgcttattta aagcgtcgtg
     4801 aatttaatgg cgtaaattcc tgctatttat tcgtttgctg aagcgatttc gcagcatttg
     4861 acgtcaccgc ttttacgtgg ctttataaaa gacgacgaaa agcaaagccc gagcatattc
     4921 gcgccaatgc gacgtgaagg atacagggct atcaaacgat aagatggggt gtctggggta
     4981 atatgaacga acaatattcc gcattgcgta gtaatgtcag tatgctcggc aaagtgctgg
     5041 gagaaaccat caaggatgcg ttgggagaac acattcttga acgcgtagaa actatccgta
     5101 agttgtcgaa atcttcacgc gctggcaatg atgctaaccg ccaggagttg ctcaccacct
     5161 tacaaaattt gtcgaacgac gagctgctgc ccgttgcgcg tgcgtttagt cagttcctga
     5221 acctggccaa caccgccgag caataccaca gcatttcgcc gaaaggcgaa gctgccagca
     5281 acccggaagt gatcgcccgc accctgcgta aactgaaaaa ccagccggaa ctgagcgaag
     5341 acaccatcaa aaaagcagtg gaatcgctgt cgctggaact ggtcctcacg gctcacccaa
     5401 ccgaaattac ccgtcgtaca ctgatccaca aaatggtgga agtgaacgcc tgtttaaaac
     5461 agctcgataa caaagatatc gctgactacg aacgcaacca gctgatgcgt cgcctgcgcc
     5521 agttgatcgc ccagtcatgg cataccgatg aaatccgtaa gctgcgtcca agcccggtag
     5581 atgaagccaa atggggcttt gccgtagtag aaaacagcct gtggcaaggc gtaccgaatt
     5641 acctgcgcga actgaacgaa caactggaag agaatctcgg ctacaaactg cccgtcgaat
     5701 ttgttccggt acgttttacc tcgtggatgg gcggtgaccg cgacggcaac ccgaacgtca
     5761 ctgccgatat cacccgccac gtcctgctgc tcagccgctg gaaagccacc gatctgttcc
     5821 tgaaagatat tcaggttctg gtttctgaac tgtcgatggt tgaagcgacc cctgaactgc
     5881 tggcgctggt tggcgaagaa ggtgccgcag aaccgtatcg ctatctgatg aaaaacctgc
     5941 gttctcgcct gatggcgaca caggcatggc tggaagcgcg cctgaaaggc gaagaactgc
     6001 caaaaccaga agggctgctg acacaaaacg aagaactgtg ggaaccgctc tacgcttgct
     6061 accagtcact tcaggcgtgt ggcatgggta ttatcgccaa cggcgatctg ctcgacaccc
     6121 tgcgccgcgt gaaatgtttc ggcgtaccgc tggtccgtat tgatatccgt caggagagca
     6181 cccgccatac cgaagcgctg ggcgagctga cccgctacct cggtatcggc gactacgaaa
     6241 gctggtcaga ggccgacaaa caggcgttcc tgatccgcga actgaactcc aaacgtccgc
     6301 ttctgccgcg caactggcaa ccaagcgccg aaacgcgcga agtgctcgat acctgccagg
     6361 tgattgccga agcgccgcaa ggctccattg ccgcctacgt gatctcgatg gcgaaaacgc
     6421 cgtccgacgt actggctgtc cacctgctgc tgaaagaagc gggtatcggg tttgcgatgc
     6481 cggttgctcc gctgtttgaa accctcgatg acctgaacaa cgccaacgat gtcatgaccc
     6541 agctgctcaa tatcgactgg tatcgcggcc tgattcaggg caaacagatg gtgatgattg
     6601 gctattccga ctcagcaaaa gatgcgggcg tgatggcagc ttcctgggcg caatatcagg
     6661 cacaggatgc attaatcaaa acctgcgaaa aagcgggtat tgagctgacg ttgttccacg
     6721 gtcgcggcgg ttccattggt cgcggcggcg cacctgccca tgcggcgctg ctgtcacaac
     6781 cgccaggaag cctgaaaggt ggcctgcgcg tgactgaaca gggcgagatg atccgcttta
     6841 aatatggtct gccagaaatc accgtcagca gcctgtcgct ttacaccggg gcgattctgg
     6901 aagccaacct gctgccacca cctgagccga aagagagctg gcgtcgcatt atggatgaac
     6961 tgtcagtcat ctcctgcgat ctctaccgtg gctacgtacg tgaaaacaaa gattttgtgc
     7021 cttacttccg ctccgctacg ccggaacaag aattgggcaa actgccgttg ggttcacgtc
     7081 cggcgaaacg tcgcccaacc ggcggcgtcg agtcgctgcg cgccataccg tggatttttg
     7141 cctggacgca aaaccgcctg atgctccccg cctggctggg tgcaggtacg gcgctgcaaa
     7201 aagtggtcga agatggcaaa cagagcgagc tggaagccat gtgccgcgat tggccattct
     7261 tctcgacgcg tctcggcatg ctggagatgg tcttcgccaa agcagacctg tggttggcgg
     7321 aatactatga tcaacgtctg gtagacaaag cactgtggcc gttaggtaaa gagttacgca
     7381 acctgcaaga agaagacatc aaagtggtgc tggcgattgc caacgattcc catctgatgg
     7441 ccgatctgcc gtggattgca gagtctattc agctacggaa tatttacacc gacccgctga
     7501 acgtattgca ggccgagttg ctgcaccgct cccgccaggc agaaaaagaa ggccaggaac
     7561 cggatcctcg cgtcgaacaa gcgttaatgg tcactattgc cgggattgcg gcaggtatgc
     7621 gtaataccgg ctaatcttcc tcttctgcaa accctcgtgc ttttgcgcga gggttttctg
     7681 aaatacttct gttctaacac cctcgttttc aatatatttc tgtctgcatt ttattcaaat
     7741 tctgaatata ccttcagata tccttaagga attgtcgtta tattcggcga tatttttttc
     7801 aagacaggtt cttactatgc attccacaga agtccaggct aaaccccttt ctagctggaa
     7861 agccctgggt tgggcactgc tctacttttg gtttttctct actctgctac aggccattat
     7921 ttacatcagt ggttatagtg gcaccaacgg cattcgcgac tcgctgttat tcagctcgct
     7981 gtggttgatc ccggtattcc tctttccgaa gcggattaaa attattgccg cagtgatcgg
     8041 cgtggtgcta tggacggcct ctctggcggc gctgtgctac tacgtcatct acgggcagga
     8101 gttctcgcag agcgttctgt ttgtgatgtt cgaaaccaac accaacgaag ccagcgagta
     8161 tttaagccag tatttcagcc tgaaaattgt gcttatcgcg ctggcctata cggcggtggc
     8221 agttctgctg tggacacgcc tgcgcccggt ctatattcca aagccgtggc gttatgttgt
     8281 ctcttttgcc ctgctttatg gcttgattct gcatccgatc gccatgaata cgtttatcaa
     8341 aaacaagccg tttgagaaaa cgttggataa cctagcctcg cgtatggagc ctgccgcacc
     8401 gtggcaattc ctgaccggct attatcagta tcgtcagcaa ctaaactcgc taacaaagtt
     8461 actgaatgaa aataatgcct tgccgccact ggctaatttc aaagatgaat cgggtaacga
     8521 accgcgcact ttagtgctgg tgattggcga gtcgacccag cgcggacgca tgagtctgta
     8581 cggttatccg cgtgaaacca cgccggagct ggatgcgctg cataaaaccg atccgaatct
     8641 gaccgtgttt aataacgtag ttacgtctcg tccgtacacc attgaaatcc tgcaacaggc
     8701 gctgaccttt gccaatgaaa agaacccgga tctgtatctg acgcagccgt cgctgatgaa
     8761 catgatgaaa caggcgggtt ataaaacctt ctggatcacc aaccagcaga cgatgaccgc
     8821 ccgcaatacc atgctgacgg tattttcgcg ccagaccgac aagcagtact acatgaacca
     8881 gcaacgtacg cagagtgcgc gtgaatacga caccaacgtg ctgaagccgt tccaggatgt
     8941 gctgaatgac cctgcgccga agaaactgat catcgttcat ctgctgggta cgcatatcaa
     9001 atacaaatac cgctacccgg aaaatcaggg caagtttgat ggcaataccg atcatgttcc
     9061 gccaggatta agcgcagaag agctggaatc atataacgat tatgacaacg ctaacctgta
     9121 taacgatcat gtggttgcca gcctgattaa agactttaaa gcggcagacc cgaacggatt
     9181 cctggtttac ttctctgacc acggtgaaga ggtttacgac acgccgccgc acaaaactca
     9241 ggggcgtaac gaagacaacc cgacgcgcca catgtacacc attccgttcc tgctgtggac
     9301 gtcggaaaaa tggcaagcga ctcatccccg tgatttctca caggatgtcg atcgtaaata
     9361 cagcctggcg gaactgatcc acacctggtc agatttggcg ggcttatctt acgacggtta
     9421 cgatccaacc cgttcagtgg tgaatccgca gttcaaagaa actacccgct ggattggtaa
     9481 cccgtacaag aaaaacgcgc tgatcgatta cgacactctg ccgtatggcg atcaggtggg
     9541 taatcagtag atgacctttg tatcgcgtct ggcgctattt gccggatgcg atactgccgc
     9601 aatgtttaca tcgaactccc ctcaggccac acttttcagg tggccttttt taataatgcc
     9661 agttactgtt ttcccgtcta catacctaag ggaataacat gtatcacgac gtcagctatc
     9721 tgctctcccg cctgatcaac ggcccgctgt ccctgcgcca gatttacttt gccagcagta
     9781 acggcccggt gcccgatctt gcgtatcagg tcgattttcc acggctggaa attgtgctgg
     9841 aaggtgagtt tgttgatacc ggcgctggag caacgttagt tcccggcgat gtgctgtacg
     9901 ttgccgctgg tggctggaat ttcccacaat ggaaaacacc cgctaccacc tttagcgtgc
     9961 tgtttggcaa acagcaactc ggcttcagcg tcgtgcaatg ggatggcaaa caatatcaaa
    10021 atctggcgaa gcaacacgtc gcccgacgcg gcccacgcat aggttctttt ctgctacaaa
    10081 cgctcaatga aatgcaaatg cagccgcagg agcagcaaac ggcaaggctt atcgtagcca
    10141 gcctgcttag ccactgccgc gatttgctcg gcagccaaat ccagaccgcc tcacgcagcc
    10201 aggcactatt tgaagctatt cgcgattata tcgacgaacg ctatgcctcc gcgcttaccc
    10261 gcgaatctgt cgcacaggca ttctatattt caccaaacta tctctcgcac ctgtttcaaa
    10321 aaacgggggc cattggtttt aacgaatacc tgaatcacac gcgactggag cacgctaaga
    10381 cgttattgaa aggctatgac ctgaaggtaa aagaagtggc gcacgcctgc ggctttgtcg
    10441 acagcaacta cttctgccgt ctgtttcgta aaaacaccga acactcgccg tcggaatatc
    10501 gccggcagta ccacagccaa ctgacagaaa aaccgactac tccagaataa gatgggtttg
    10561 ctgcggcgca gaaagcactt tgcgcaccgc gctcattacc cgctgcggct cacgcaggaa
    10621 tgcgtagatg ctgcattgca catagcggca atgttcaaat cgctcggcac cggcaagctc
    10681 gatatccgta atcaacagag caacatccgc ccgacggata tcctcgtccg ctaaacgatt
    10741 ctccgttccc agcgcgccct gagtttcaat gctgacgccc cacttctcta actggcacaa
    10801 cttttccagc cgttccgccg ccatataagt atgcgccacg ccgcttacgc aggcggttac
    10861 tgccaccagg tatgccattt taacctccca cggtaacctg aaatccggcc cgttctgcca
    10921 tttcgcgcat cgttgtcaca tcggctgacg acggcgcagg cacctctttc atcgaccatg
    10981 ttttccccag caggcggtat ttcggttcgc cgtactgatg aaacggtaac agatggatct
    11041 gcctgatttt cagcggggtc agtacatcca gcgcctgctg catattctcc cggctgagcg
    11101 tgaaaccagg gatcaacggt aaacgcggga tcacgttgac accctcactc accagcaaac
    11161 gcagattctc cagcacgcgt ggcaggttca tcttcaccac atcccgcgcc tgagtcgcgt
    11221 ccataatttt taaatcgaac agcacttcat cgcacaattt cgccagcggt aacagcttgc
    11281 ttgctggcgc gtctccggca gtttcaatgg cgcatgagac accccacagc cgcagtcgct
    11341 gtaaaaaacg ggtagcaaac tccgcctgca ttaacacttc gccgccagaa agcgtgacgc
    11401 cgccgccgga cgtgcgaaag aaaatgtcat ctttcatcac ttcccgttcc agagcgtcaa
    11461 ggctgatatc gcgaccaatc cgttcaaacg ccccggaggg gcattcatcc gcatcacgca
    11521 aacatttcgc acagtgcaga catttcgcct ctctgcgtac cgtctggatt ttgccggaga
    11581 tcgactccgg attagcacac cacgggcaaa gatgcggaca gcctttaaaa aagaccaccg
    11641 tacgaatgcc ctcgccgtca ttcagtgaat aacgctgaat gttgaaaatg cgcgccacat
    11701 catcgcggcg cgtttccaca acgttacagc tgatgcgctg tccggcggat gatgtcatcc
    11761 tggatctcct tcgacagttc gacaaagaag gcgctgtatc cggcaacgcg caccaccagc
    11821 ccggcataat cttgtgggcg ctgttgcgct tcccgcaacg tgtcggcgtt caccacgtta
    11881 aactgaatat gctgcaactt aagctgggta aacacccgta agaagtcggc cagtttgcgt
    11941 aatcccgctt caccttccag ggtcgccgga gtgaatttca cgttcagcaa cgtaccgtta
    12001 gacagcagcg tattatcgag cttactgact gacttcagta ccgccgttgg cccttgtgcg
    12061 tcctgaccca gcataggtga caagccgccg tctgccagtt gttccccggc aaaacgaccg
    12121 tctggcgtcg cgccaaccac cgatcccaac ggaacgtgag cagaaacggt atacgatccc
    12181 ggcgtgaagt aaccgccgcg cgggttctgg tatttttcca cttctttgca gtagtggcgc
    12241 aacagttcgg cgctaatgtt atccacctcg tcgatatcgt taccgtattt ctcaaagcgg
    12301 ttaattaagc gagcgcggac tttttcgcct tctggcgttg caaagttggc ttttaatacc
    12361 gacagcaatt cgtcaaaact taaacgctgt tgctcaaaaa ccatcccttt caacgcatgg
    12421 agagaatcgc tcaggttggc gataccaatt ccctgtacgc cggagaagtt ataacgcgcg
    12481 ccgccatcgg taatatcgcg acctttttcc agacaatcgc tgataaaaga cgagagcagc
    12541 ggtacaggtg cccagtcgcg atggccgata tcacaaatat tgctgccctc aaccatcagg
    12601 gtgatgtagt ggctgatctt ggcgcggatc tgctccagta aaccttcata agtcagcgca
    12661 gcattgcctt cattttcatg caggcagatt tccatcactt tcagcagatt aaacatcgcg
    12721 atgtcatgca agccgtaggt tctgccggga atagataatt ccacacagcc cactacggaa
    12781 tagtcgcgcg catcttccag cgaaacgcca cggttgagga acgctggcac caccacttca
    12841 tcgttaaaga tttgcggaat accggtgccg aggcgaatgg tttcggcggt tttcatcagg
    12901 aacggcgtgt cgatcaaggc gttagtgcgc acgccgaggt tcggttgcgg taattgcacg
    12961 ctttgatagg cgtcaaggca aaggaacgaa agcacgttca ccgcgctacg tccgttctcg
    13021 gttaacccgc cgagcagtgc ggtatagccg gtcgggaaac cggcgaaata acgcgcgcta
    13081 ctggtggagc gcaacagcac gatgtcgttg catttcaccc ataaagattc gagcagttct
    13141 ttcaggaacg ccggatcttc gccctgggtt aaagatgcct gatagaacgg caacatatac
    13201 tggtcgaagc gccctaaaga cagcgaactg gcgttggatt cgtattgcag aatgatgttc
    13261 atgtaccaga ataactggca cgcctgccag aacgtctgcg gcttatgttg cgcgttatgg
    13321 cgggagatct ccgcaatagt cagcagctct tcgcgacgct gggcatctgt gcagtttgcc
    13381 gccatcgttt ccgccagttc ggcgtaacgc aaaatgtgtt tctgcgaggc ttccagcagt
    13441 aacaacgcgg cctgataaaa gtgattctcc ggctgttgct gacaatgttg ctgcatctgt
    13501 gccaccagct cacccagacc gtgattcaac aggcgtgggt aatcaataat aatgtgcccc
    13561 tggcctttat ccgtctggtt gatgctgaaa atctgcgtgt tggtcgcggc ttttacttca
    13621 tctgtcatct gcccgttgat gaaatctttc atcgaacgtt tttcccagta cgggaacaac
    13681 tcttcacggt agatacgttt atcttcttcg ctgatagcaa agcggtcctg cggacgcgtc
    13741 gggaattgat ccagctcttt cagcagccag taagggtcca tttccggcga cataatcccg
    13801 gcgcgcggtt ttacggtgcg gttaccggca atcagttctt catcacgaat cgaaatttca
    13861 acatgttcaa ggatatacgc tgttgctttc gcccggcgca atatcaccgg ttcgccttcg
    13921 gtttgccgat ggctggcggt ataaagcagc gcccgctcca gcgagatttc acgggtattg
    13981 gcaaacagtg cagttttgag gcgagagata cgattcgtca tgaggaactc ctgtgggaaa
    14041 gcgaacccgc ccttgcgagc gggagaagaa cttaagcggt ttgcgccagg tgagcttcaa
    14101 ttttgctcat aatggcgtca gcacgtttta ctgcatcgct gatgttgacg cgcacgatgg
    14161 tcttgccagc aaaacgctct tcaaacttga tgccgatatc tttggtcaga atcaccatat
    14221 cagcgctcgc cacgtcttcc gcagtcagtt cattttccag accaattgac ccctgggttt
    14281 ctactttcac ttcccagcct ttcgctttcg cggcactttc cagcgcctct gctgccatat
    14341 aggtatgggc aacacctgaa ggacatgcgg ttactgcaat aattttcgtc atggctcgtt
    14401 cctcaattaa ttaatttcaa aatccaaatc caggtcgtct tctttttcat cagtcgaaga
    14461 cccattttta cgcgccagac ttttcagcac gttcacacaa acagccgtca ccactgctcc
    14521 gacagccact gctgcgatat aacccaactt accttcaacc accggcagca caatcagacc
    14581 accccagcct gcatagcact gcgcaccgac cagcgccgca gttactgcac cacaaactga
    14641 accgaccata atggaaggaa tgacacgcag cggatcggca gcggcgaaag gaatcgcccc
    14701 ttcagtaacg ccaacacagc ccatcaccag tgccgctttg ccggtttcgc gctcttctgc
    14761 ggagaaattt ttacgaccaa tcagcgtcgc cagccccatc cccagcggcg ggatacaaat
    14821 acccacggca gcaatggcca ccacggtata aacaccctga gcaacgcaaa tcagcatgaa
    14881 ggcataggcc actttgttaa ccggaccgcc catatcgaac gccagcatca gccccatgat
    14941 caccgccagc ataacaatgc tgccctgctg catcccctga agccactgag tcaggctgtt
    15001 ggtcaacgcc cctacaggct cgcccaagcc ccacatcatg atgcctgcgg taatcagtgt
    15061 gccgatgata ggaataatga agatgggcat caccgagcgc agaactttat gcaccggaat
    15121 tttcttcagg taatgcacca cgatgccgcc gataatcccg gcgatcagcg caccaaagaa
    15181 gcccgcacca aagctgttac caacccaggc accgatagcg cacggagcca gcgcagaacg
    15241 ctctgcaatg gagtaaccaa tgtaagcggc gaggaaaggc accatcagcg tcaaacctgc
    15301 aacgccgata tcaaacagtt ttttcaaatt cggatcggct acggcatccg gcactgcgcc
    15361 tttgccatac aacatgacgg aaaccgccag caaaataccg cccgatacca cgaagggaat
    15421 catgtgtgaa acgcccgtca ttaaatgctg acgggtattt ttcaggatct gcaccaactc
    15481 attcataagc tgctcccggg cttgcaaaag cccatgtcca tatttttgat ggaggaaaca
    15541 ataggcaatc gcaggcggag caaacagtgg ataaaaaggc catttactgg acttttgtaa
    15601 tatcagtaca aaaatgcgat ccgcctcata acttgcgata atccggaagc atgccgcaaa
    15661 acggcgtctt gtgagacgcg tcgcaaattg acggttacat tacatttgtc cagcttttgg
    15721 cagatttgtc acatggcagg ccgttaccgt gcgctcttat cctgtaaaca gaaaaccgta
    15781 ccgggagaaa ggcaatggcc ctgattgtgg aatttatttg tgagctacct aacggcgtac
    15841 atgcgcgtcc ggcaagccac gttgaaacgc tgtgtaatac tttttcatct caaattgagt
    15901 ggcataacct gcgtactgac cgtaaaggca acgccaaaag cgctctggcg ctgattggca
    15961 ccgatacgct ggtgggtgat aactgccagc tactgatttc cggggccgac gaacaggaag
    16021 ggcaccagcg tttaagccaa tggctgcgcg atgaattccc ccactgcgat gcgccgctgg
    16081 cggaagttaa atctgacgaa ctggaaccac tgccggtttc actgaccaat ctgaatccgc
    16141 aaattatccg cgcccgcacc gtgtgcagcg gtagtgcagg cggcattctg acgccgatct
    16201 cttctttaga tctcaatgcg ctgagtaatc ttcccgcagc caaagacgtt gacgccgagc
    16261 aatccgcact ggaaaacggc ctgacgctgg tactgaaaaa cattgagttt cgtctgctgg
    16321 atagcgacgg tgctaccagc gcgattctgg aagcgcaccg atccctggct ggcgatactt
    16381 ccctgcacga acatttactg gcaggcgtca gcgccggatt aagctgcgcc gaagcgattg
    16441 tcgccagtgc gaatcacttt tgcgaagagt ttgcccgttc cagcagcaga tacctgcaag
    16501 aacgtgccct ggacgtacgc gacgtctgct tccagttact ccagcaaatc tacggtgagc
    16561 aacgcttccc ggcaccgggc aaactgacgc agtccgccat ttgtatggct gatgaactga
    16621 cccccagcca gttcctcgaa ctggataaaa atcacctcaa aggattgtta ctgaaaagcg
    16681 gcggcaccac ctcacatacg gtgatccttg cccgttcgtt caacattccg acgctggttg
    16741 gtgtggatat tgatgccctc actccgtggc agcatcaaac gatttatatc gacggcaacg
    16801 ccggggcgat tgtggttgag ccaggagaag ccgtagctcg ttattatcag caagaagccc
    16861 gcgtacagga cgccctgcgt gagcaacagc gtgtctagct gacccaacaa gcccgtaccg
    16921 ctgacggtat ccgcattgaa attgccgcta acatcgctca ctccgtggaa gcgcaggccg
    16981 cattcggcaa tggtgcggaa ggcgttggtt tgttccgcac tgaaatgctc tatatggatc
    17041 gcaccagtgc accgggcgaa agcgagctgt acaacatttt ttgtcaggcg ctggagtctg
    17101 ccaacggacg cagcattatt gtgcgcacta tggacattgg cggcgacaaa cccgttgatt
    17161 atctaaacat tcccgcagag gcaaacccgt tcctcggtta tcgcgccgtg cgtatttatg
    17221 aagaatacgc gtcgttattt accacacaac tacgctcgat cctgcgcgcc tccgctcacg
    17281 gcagcctgaa aatcatgatc ccgatgatct cctcaatgga agagatctta tgggtgaaag
    17341 aaaaactggc agaagccaaa cagcaactac gtaacgaaca cattccgttt gatgagaaga
    17401 tccagctcgg cattatgctg gaagtgccgt cggtgatgtt catcatcgat caatgctgcg
    17461 aagagattga tttctttagt attggtagta atgacctgac gcaatatctg ctggcagtgg
    17521 atcgcgataa cgctaaggtt actcgtcact acaacagcct gaatccggca ttcttgcggg
    17581 cgctcgatta cgacgtgcag gcggtgcatc gccagggtaa atggattggt ctgtgcggtg
    17641 agctgggagc gaaaggttcc gtgctgccgt tgctggtcgg cttagggctg gatgaactca
    17701 gcatgagcgc accatcaatt ccggcggcga aagcgcggat ggcgcaactt gatagccgtg
    17761 agtgccgcaa gttgctcaac caggcaatgg cctgccgtac ttcgctggaa gtggaacacc
    17821 tgctggcgca attccgcatg acccaacagg acgcaccgct ggtcaccgcc gagtgcatca
    17881 cgctggaaag tgactggcgc agcaaagaag aagtgctcaa aggcatgacc gataacctgc
    17941 tgctggcggg ccgctgccgc tatccgcgta aactggaagc cgacttatgg gcgcgcgagg
    18001 ccgttttctc taccggtctg ggctttagtt ttgccattcc acacagcaaa tcagaacaca
    18061 ttgagcaatc caccatcagc gtggcgcgtc tgcaagcgcc ggtgcgctgg ggcgatgatg
    18121 aagcgcaatt catcattatg ttaaccctga acaaacacgc tgcgggcgat cagcacatgc
    18181 gcattttctc gcgcctcgct cgccgcatca tgcacgaaga attccgtaac gcgctggtta
    18241 acgccgcctc tgccgacgct atcgccagcc tgctgcaaca tgaactggaa ctgtaaaagg
    18301 aaacatcatg gaactgtatc tggacaccgc taacgtcgca gaagtcgaac gtctggcacg
    18361 catattcccg attgccgggg tgacaactaa cccgagcatt atcgctgcca gcaaggagtc
    18421 catctgggaa gtgctgccgc gtctgcaaaa agcgattggt gatgagggca ttctgtttgc
    18481 tcagaccatg agccgcgacg cgcaggggat ggtggaagaa gcgaagcgcc tgcgcgacgc
    18541 tattccgggt attgtggtga aaatcccggt gacttccgaa ggtctggcag caattaaaat
    18601 actgaaaaaa tagggtatta ctacacttgg caccgctgta tatagcgccg cagaagggtt
    18661 attagccgca ctggcagggg caaaatacgt tgctccgtat gttaaccgcg tagatgccca
    18721 gggcggggac ggcattcgta cggttcagga gctgcaaacg ctgttagaaa tgcacgcgcc
    18781 agaaagcatg gtgctggcag ccagctttaa aacgccgcgt caggcgctgg actgtttact
    18841 ggcaggatgt gaatccatca ccctgccctt agatgtagcg caacaaatgc tcaacacccc
    18901 tgcggtagag tcagctatag agaagttcga acacgactgg aatgccgcat ttggcactac
    18961 tcatctctaa aggagcaatt atggaccgca ttattcaatc accgggtaaa tacatccagg
    19021 gcgctgatgt gattaatcgt ctgggcgaat acctgaagcc gctggcagaa cgctggttag
    19081 tggtgggtga caaatttgtt ttagtttttg ctcaatccac tgtcgagaaa agctttaaag
    19141 atgctggact ggtagtagaa attacgccgt ttggcggtga atgttcgcaa aatgagatcg
    19201 accgtctgcg tggcatcgcg gagactgcgc agtgtggcgc aattctcggt atcggtggcg
    19261 gaaaaaccct cgatactgcc aaagcactgg cacatttcat gggtgttccg gtagcgatcg
    19321 caccgactat cgcctcgacc gatgcaccgt gcagcgcatt gtctgttatc tacaccgatg
    19381 agggtgagtt tgaccgctat ctgctgttgc caaataaccc gaatatggtc attgtcgaca
    19441 ccaaaatcgt cgcgggcgca cctgcacgtc tgttagcagc gggtatcggc gatgcgctgg
    19501 caacctggtt tgaagcgcgt gcttgctctc gtagcggcgc gaccaccatg gcgggcggca
    19561 agtgcaccca ggcggcgctg gcactggctg aactgtgcta caacaccctg ctggaagaag
    19621 gcgaaaaagc gatgcttgcc gccgaacagc atgtagtaac cccggcgctg gagcgcgtga
    19681 ttgaagcgaa cacctatttg agcggcgttg gttttgaaag cggcggctgg cggcggctca
    19741 cgcggtgcat aacggcctga ccgctatccc ggacgcgcat cactattatc acggtgaaaa
    19801 gtggcatttg gtacgctgac gcagctggta ctggaaaacg cgccggtgga ggaaatcgaa
    19861 accgtagctg cacttagcca tgcggtaggt ttgccaataa ctctcgctca actggatatt
    19921 aaagaagatg tcccggcgaa aatgcgcatt gttgcaaaag catcttgtgc agaaggtgaa
    19981 accatccaca acatgcctgg cggcgcgacg ccagatcagg tttacgccgc actgctggta
    20041 gctgaccagt acggtcagcg tttcctgcaa gagtgggaat aacctactcc aaactcccgg
    20101 cttgtccggg agtttgaacg caaaattgcc tgatgcgcta cgcttatcag gcctacgcaa
    20161 tctctgcaat atattgaatt tgcgtgcttt tgtaggccgg ataaggcgtt cactccgcat
    20221 ccggcactct gtgccagtaa tctgaaactc ccacgttgtt caggagtttt ttatccgctt
    20281 ctggacattt tctccacaga acaatcgtta gcccggaagg tcgaatctgt gactcttatt
    20341 ttcacggacg aaccagatga aagcttcact ggcgctgctc agcctgctaa cggcatttac
    20401 cagccactcc ttaaaatctc ccgccgttcc accaaccgtg gtacaaatcc aggccaatac
    20461 caaccttgcc atcgcagacg gtgccagaca gcaaattggc agcacgctat tttacgatcg
    20521 cagcttacct atcctggcgg tgatgttccg caagaacgcg gtgtatgttc cgatatggtg
    20581 atccgcgcat tgcgcagcca gaaggtcgat ttgcagaaac tggttcacga agatatggcg
    20641 aagaattttg ctgagtaccc gcaaaaatgg aagttaaagc gcccggacag caacatcgat
    20701 caccgtcggg tgcctaatct ggaaacctgg tttagccgcc acaacaaaac acgccccatc
    20761 agtaaaaatg ccggtgacta tcaagcgggc gatattgtct cctggcgatt agataacggg
    20821 ctggcgcata ttggggtggc gtcagatggc ttcgcccgcg acggtacgcc gctggtgata
    20881 cataacattg gcgcaggcgc gcaggaagaa gatgtgctgt ttagctggcg gatggtgggg
    20941 cattatcgtt attttgttaa ataagagatc gccccacatg aagaaaaaat tcagatgctt
    21001 tttacactga cagcttcttt tttcttacgg ctcaccagcg ccagtgccag cacaatcagc
    21061 acaataccgc taccttcaac ggcccccgga ttctcaccga gcagccacca ggaaaagagt
    21121 acgccacaaa cgggaacggc cagtgtgctt aagctggcaa tactggcagg caagtttttc
    21181 agcacaaaca gccataagct ccacgctagc gccgtcgcca gaatcgcact gtaggccagc
    21241 gcccagaaca cggtgggctg ccagtcaatt tcacgttgcg gcaccagtaa agcgaccaca
    21301 ctcatcacca gcgccgcata cagcatctgc caggatgtta acgataataa atccacgcgc
    21361 ggatgacggg cgtataggcg tttagcaaca atcgcgctcg ccccccaact aacaccggag
    21421 aggattgcca gcatggcact tttcatcgaa gagaaatcga gttgccacgg ctgcaacacc
    21481 aaaaataagc cgaaagcggc aatcagaatc gcgaaatatt gcccacgtcg caggcgttca
    21541 ccgagaaaca acgcggcgaa aatcaccacc cagaacggca tggtatagct caggatcgcc
    21601 accttccccg cacctccgct gaccaacgcc cactgcgcca gaccaaccat cccgcaggtt
    21661 tgtaacaggg caatggctaa ggtgtattta aacggcgtcg ggcgcattcc gcgaccacgt
    21721 aataaaagga cgatgaataa aacgagagcg ccgaaaatgc agcgtaaggc ggtaaagtcg
    21781 aaggcaccga tgtaactggt gacttgcttc atgaaaatcc agctataact ccagataagt
    21841 gtgagcacaa ccaagccact gattgccagt gggttgctct ttcctgcggc agacatgaga
    21901 gatccagtgt gtagttttcc tgtggacact atacgccggt aaagaaactt cagcgactgc
    21961 ctgaaagaca gtcgctgaag aaggtcagat tacagcaggt cgaaacggtc gaggttcatc
    22021 actttcaccc atgccgctac gaagtcttta acaaacttct cgtgggcatc gctgctggcg
    22081 taaacttccg ctactgcacg caggacagag ttagaaccaa ataccagatc cgcacggctg
    22141 gctgtgtatt tcacttcgcc ggtttcgcgg tcacgtcctt cgaacagctc tttcgattcg
    22201 tcagtcgctt tccactcgta acgcatatcc agcaagttca cgaagaagtc attgctcaat
    22261 acgccaacgc ggtcagtgaa gacgccgttt ttgctgccat cgaagttggc acccagtaca
    22321 cgcatcccgc ccaccagcgc agtcatttcc ggcgcggtca gcgtcagttg ctgtgctttg
    22381 tcgatcagca gtgactcggt ggtggaaacg tccagacgag cgcgatagtt acggaaaccg
    22441 tcagcaattg gctccagcag ctcaaacatc tcaatgtcag tctgatcctg acgcgcatca
    22501 acgcgacccg gcgcaaacgg tacatgaatg ctcaaacctg cggcgcttgc ggctttctca
    22561 acaccaacca caccagccag cacgatgata tccgccagcg aggctttacc agactctttc
    22621 tggattttct ccagaacagg cagagcacga acggctgcgg cgttcacatc ccagtcgcgc
    22681 tgcggcatta atgccagacg cgcaccgttg gcaccaccgc gtttgtcgcc accacggaag
    22741 gtagaagcag atgcccaggc caccgatacc agctcactaa cagacagacc agaatccgca
    22801 atcgcgaatt tcagatcgat aatgtcctgc tcggtcgggt tgtagatcgg ctgcggcagc
    22861 ggatcttgcc agatcagatc ttctttcggc acttccggcc cgatgtagcg agatttcggc
    22921 cccatatccc tgtgcgtcag tttgaaccag gcacgggcaa aggcttcgtt gaacgcctgc
    22981 ggatcgttga ggaaacgacg agagatcttc tcgaactcag gatcaaaacg cagcgtcagg
    23041 tcggtcacca gcattgtcgg tttacgtttc ttcgacggat cgaacggatc cgggataatt
    23101 tccggtgcgt ctaccgcttc gaactggatt gctccagccg gactgcgggt ctgtacccac
    23161 tcatacttga acagattctc gaagaaatag ttgctccact ggatcggcgt ctgggtccag
    23221 actacttcca gaccagaggt aatggcatct gcgccaacgc cgctgccgta agtgctcgcc
    23281 cagcctaaac cttgttcttc aatcggtgca gcttctggat caggacctac gtttgatgtc
    23341 ggaccggcac cgtgggtttt acccagcgta tgaccacccg caatcagcgc cacggtttct
    23401 tcgtcgttca tgcccatgtt gccgaaggtc gcgcggatag ctgctgccgc agaaagcggt
    23461 tcgccgctgt gatccgggcc ttccgggtta acgtaaatca ggcccatctc ggttgcaccc
    23521 agcggtgctt tcgccagcgc ttccggatga cggtgagtca gccaggcttt ttcatcaccc
    23581 cagttaacat ccagatccgg ttcccagacg tcttcacgac cggcaccaaa accgaaggta
    23641 cggaagccgg agttttccag cgccacgtta cctgcgagga taaacaggtc ggcccaggag
    23701 attttctgac catatttctg tttgattggc cacaacaggc gacgcgcttt atcgaggctt
    23761 acgttatccg gccaggagtt cagcggtgca aaacgttgct gaccacgacc cgcgccgccg
    23821 cgtccatcga ttgaacggta agtacctgcg ccgtgccaag ccatacgaat aaacagaccg
    23881 gcgtagctgc cccagtcggc tggccaccac ggctgagatt ctgtcagcag ggctttcaga
    23941 tcttttttca ggccgtagta atctaatttg ctgaattctt tgcggtagtc aaagtcctca
    24001 cccagcgggt tagaacgatt agaatgttgg tttaacaggt caacacggag ttgatttggc
    24061 caccagtcgc gagtggttgt gcccgccccc gcgctctggt cgtgaccgcc ctgatggaac
    24121 gggcatttgc cagtggctgt ggtgttatgg atatcgtctg acgtgctcat caatgtgctc
    24181 ccctctacag tgttaccgtt acgatacaca gcgttagaga gaagttataa ttgaattaat
    24241 ccacggatgc gatagttgag atcttacata ttgttggtta aagagatgta gatcaaattg
    24301 atcgtaatta agcttttttg attatgtccg aattcggaca aatgctttat aaaaaagggt
    24361 tttagcttca tatccttcaa gtaatgaggg agaaaacaag gctgacaacc ttattttccc
    24421 gccctcattt cgaggcagca ttttgtgctc tgtttaaaat ttgtgatcac tgtgtgattt
    24481 tcacaaaagc cacactattt ataaaccagg tcgaacaccc agcgtatggc aaatcgcgta
    24541 actcatttca gcgcggttaa gcgtatagaa gtggaaatct ttcactcctt cacggcttaa
    24601 aatcttcacc atatccatgg caatattcgc gcccaccagt ttgcgggttt cggcatcgtc
    24661 atccagcccg tcaaacattt gcgccatcca cgccggaata cgcacgttgg tcatgtcggc
    24721 aaatttcttc gcctgtttaa agttagatac cggcaaaatg cccgggatga tttccacatc
    24781 aatgcccgcc gatacacagc ggtcacgaaa acgcaggtag ctttcgacat cgaagaagaa
    24841 ctgagtaatc gcgcggttgg ctccggcatc cactttgcgt ttcagattaa gcaaatccgc
    24901 ctgagcgctt tttgcttccg ggtgaacttc cggatacgcc gccacggata tatcgaaatc
    24961 tgccacttct tttaacagcg tcaccaggtc agaagcatac atttctggct taccacttcc
    25021 cggcggcaga tcgccacgca gtgccacgat atgacgaata ccgttattcc agtagtcgcg
    25081 tgcaatggtg cgcagctcgt cgggcgtcgc atcaatgcaa gtaagatgcg gtgccgcttc
    25141 cagaccagtg cgatctttaa tgcctttaat aatgctgtgc gtacggtcgc gctcgccgga
    25201 gttcgcgcca taggtcaccg atacaaactt cggtttcagg ctgctaaggc gatcgatgga
    25261 gttccacagg gtctgctcca tttcactggt acgcggcggg aaaaactcga acgaaacgtt
    25321 aatctgcccc tggacttctg ccaggctctg attcagggca tcccgctggc tggcgtgaaa
    25381 aaagctcata ccttacctca tcaatcgctt gtagttatat gttgtgttgt gcacatctat
    25441 ccatttagac gtccagatgt aaagatgaag gaaaagccga agggcgtcaa ccgaaaaatg
    25501 accaatgatg gtgaggaaaa atcagggaag atgaaaaaac ttcaggatgg taagaaaaag
    25561 aaaatccgat atgcaaagca aaccggactg caaatataaa ctaaccatgt acttattgct
    25621 tcttgccgga ttcgtcgtaa acgcttcatc cggcctaatt ctatgataat tcaatatatt
    25681 tcattagaaa ccatagaccc gaaaacatga gtaccgggca ttattaaatt tctgaaatta
    25741 caacaactgt gccagccggt tgatatccga ctgaatcgcc ccggcggtga cgtcgcgccc
    25801 agcgccaggc ccacggatca ccagcggatt atcgcggtac cagcggcttt cgatggcaaa
    25861 gacgttatcg catggcagca gtgatgccag cggatagtct tcacgcaccg cctccacgcc
    25921 cacacgcgct ttgccgttgg catcgaaacg cgccacgtag cgcagcacca gccccatttc
    25981 gcgggccgct tccagccgtt gcaccatctg ctcgttaagt tcatcgccat tttcaaagaa
    26041 atggtcgatg ctgccgcctt cgcaatgagc aggcaccagc gattccacac gtacctggtc
    26101 cggttcaatg ttgtaacccg cttcacgcgc cagaatcacc agcttgcgca ttacgtcttt
    26161 gccggagagg tcgtcacgcg gatcaggctc ggttaagccc tgctgccacg cctgatccac
    26221 cagctcggta aacggcacgc taccgtcgaa ttgcaggaac agccaggaga gcgtgccgga
    26281 gaagatccca ctgatcgaca aaatggtatc gccgctgtcg atcagatcgc gcacggtatg
    26341 gttgatcggc aagcccgcac caacggtggc gttgtatagc cagtgacgcc cggttttttc
    26401 gaaggcgtcg tggatctggc gatatttatt gctgtcgctt gctcccgcca gtttgttggc
    26461 actgatgacg tggaaaccgt ggctggcgaa atcgagatac tgatcagcaa gctgctggct
    26521 ggcggtaacg tccaacacca ctaaatcatc atacggatgg gcgcgcatcc acaggaaaag
    26581 cgactcttca tcctgctcga ccgcttcatc gttgaagaag gctaacgcgc ggctggcgtc
    26641 cagcccgtca taactcaaca gactgcggcg gctgtccacc acgcctgcca gcacaaattc
    26701 aaagccggta cgcgccgaaa gcgtgctctg ctcgcgggcg aacagttcca gccagcgaga
    26761 gccgatattg cctttgccga acaataccag gccgatgcgt ttttctgcgc ggaagacgga
    26821 ctgatgcagc ccctgaatca ggctttcggt cgggccggtg cgcagtactg ccaccagact
    26881 gatgccgtca tcggactgcc acgtaaactc gaccggctgg cctttcagtt gctgccagaa
    26941 gcggtggcaa tgcagcgggt tacgggtgac acccgccccg accatcgcca ccagcgccag
    27001 cccctgacgc aggcgcagtt ctccaggtaa tcccgcttcg tcgaggattt tcagcgcact
    27061 gtcggccact tctgaggtgt agcaaaattg caacaactgg cgatcgttat gcacgccaac
    27121 cgccagtgga cgcacctggg cacgtttcag gatctggtcg atctctttat gcgccagttt
    27181 gaaatcctga ctggcgggca cctgaaactc aatcaaacag acatcatcgt ggctggtgac
    27241 aatacgcgca ccagtaccgg aggccagcac gcgttcaatg cgcgtggaac cttgatccgg
    27301 cgtgtagcta cagcgcagct gcaggtcgat ttcgctgcca gaaactggct gtaaagtacg
    27361 ggcgtgaaga acgggagccg ccagacgcgc cagttcgctg gcctcatcca gacgcagcaa
    27421 cggcagcagg caggcatctt tcactttacg cgggtcggca ctgtataccc cggcgacgtc
    27481 gctccagatg gttacacgag aaacacccgc cagcgcaccg atttgtgtcg cggaatagtc
    27541 ggaaccgtta cgccccagca acaccgtttc accggcgttg ttacggctga taaacccggt
    27601 caccaccaga cgtttgctcg gatgttgtac cagtagctgt tgcagcaacg ggtaagaaag
    27661 cccttcatca acctgcggtt gcgcagcgcg ttcagcgcgt aaaaactcac gggcatcaag
    27721 ccaggccgct ggcagccctt gttgattaag taccgcagac atcaggcgcg ccgaccacac
    27781 ttcaccgtgg cccaccactt ccgcatacac cgcatcgtta ataccactgt cgagcaacgc
    27841 cgccaggcgc tcaagatcgc tgacaaaagc gctaatgagg ctatcggctt cttcagcggg
    27901 tagcagaccg ctaatcagat cgcactgata gcgacgcagc gtttgttgaa cctgatgcgc
    27961 agagagacga tcggtctggc ttagtttcaa ccagttaatc aactggttag tggtgctacc
    28021 ggcggcggaa accaccatca tatcgtcagg ctgagagaac tccgccataa tgcccgcgac
    28081 acgcaaataa cacttcacat cagccagact actgccacca aatttatgca gctgacgacc
    28141 tttcgcccct gcctgcgcaa tcacactcat ttttacccct tgtttgcagc ccggaagcca
    28201 ttttccaggt cggcaattaa atcttcgcca tcttcaatac cggtggagat acgcagcagc
    28261 gtctcggaga tcccggcggc agcacgcgct tctggtgcca tgcctgcatg tgtcatggtt
    28321 gcggcgtgag agattaaact ttccactccc cctaatgatt ccgccagcgt aaacaacgac
    28381 agcccgccca ggaaacgaca cagcgtctgc tcatcgccat ccagttcaaa actcaacatt
    28441 gcgccaaagc ctttttgctg acgcgcggca atttcatgcc cctgattttc cggcagcgac
    28501 gggtgataca gttttttcac caacggctgg gtttgcagat atttcacaat cgcctgcgcg
    28561 ttgcgctgcg ccagctccat acgcggcacc agcgttcgca acccgcgtag cagcagatag
    28621 ctgtcaaacg cgccgcccgt cacgccaata ttgtttgccc accaggcaag ttcagtgaca
    28681 acgtccgggt ctttagcaat caccacgcca gccaccacgt cagagtgacc gttcagatat
    28741 ttcgtacatg aatgcaacac cagatcggca cccaatgcca gcggattttg taatgccggg
    28801 cttaagaagg tgttatccac cacgctcacc gccccgactt cccttgccag atggcagatt
    28861 ttcgcaatat ccacgacgcg taacaatgga ttacttgggc tttctaccag taccagtttg
    28921 ggtttttctg ccagcgctgc ccgtaatgcc tgttcatcgc cttgatcaac aaacaacacg
    28981 cgatagcaac cgcgtttcgc cagactgtcg aacaggcgat agctaccgcc gtagcagtcg
    29041 tgcggcgcaa ccagcagatc gccaggtttc aaaaagacgg tcgttaccag gtgaatcgcg
    29101 gacatgccgg tattagtaag tactgcgcca gcaccgcctt ccagttctgc cagcgcacgc
    29161 tgaaccacat cgcgcgttgg gttgccgcga cgcgagtaat catgcgcgcg cggttcatta
    29221 aatccggtaa agttataggt gctggaaaga tggatgggag ggacaacgca accatactgt
    29281 tcgtcgtcat ttaacccgct acgcactgcg atggtggcct gtttacgcgt catgtgatga
    29341 agtttcctgg gctttgtcgg tgaaatgtca ggcaccagag taaacattgt gttaatggac
    29401 gtcaatacat ctggacatct aaacttcttt gcgtatagat tgagcaaatc ccaaatagcc
    29461 gttaaaatta tatgcattat cacgccgaca ggtgcattac acgatgtcac ggtaacgcct
    29521 gtacggtaaa ctatgcgggt ttacggtcag tacccacata aattgtgggg tctggtctca
    29581 atttattgac gaagaggatt aagtatctca tggctgaatg gagcggcgaa tatatcagcc
    29641 catacgctga gcacggcaag aagagtgaac aagtcaaaaa gattacggtt tccattcctc
    29701 ttaaggtgtt aaaaatcctc accgatgaac gcacgcgtcg tcaggtgaac aacctgcgtc
    29761 acgctaccaa cagcgagctg ctgtgcgaag cgtttctgca tgcctttacc gggcaacctt
    29821 tgccggatga tgccgatctg cgtaaagagc gcagcgacga aatcccggaa gcggcaaaag
    29881 agatcatgcg tgagatgggg attaacccgg agacgtggga atactaaagc ggaaaagagc
    29941 ggcgcggagt ggaatcgcct gatgcactac gcttatcagg cctacgtcat attgcaattt
    30001 attgaatttg cacgaacttg taggccggat aaggcgttca cgccgcatcc ggcataaaca
    30061 acgagcacgt tgtctgcgac ccaccgcttt ttatacatgg acgtttaact atgaaaaaca
    30121 ggctgctgat cctcagcctg ctggtttctg tacctgcctt tgcctggcag ccgcaaactg
    30181 gcgacatcat ctttcaaatc tctcgctcat cgcaaagtaa agcgatccaa ctggcaaccc
    30241 attccgatta cagccacacc ggtatgctgg tgatgcgtaa caaaaagccc tacgtttttg
    30301 aagcggtcgg cccggtgaaa tacaccccgc tcaagcagtg gattgcccat ggtgaaaagg
    30361 gcaactacgt tgttcgccgc gttgaaggcg gactgagtgt tgaacaacag caaaaactga
    30421 cgcaaacggc aaaacgttat ctcggtaaac cgtatgattt cagcttctca tggagcgacg
    30481 atcgccagta ctgttcggaa gtggtgtgga aggtttacca gaacgcgctg ggaatgcgcg
    30541 tgggcgaaca gcagaagttg aaagagtttg atctcagcaa cccgctggtc caggcgaagc
    30601 tcaaagaacg ctacggtaaa aatattccgc ttgaggaaac ggtagtttca ccacaagccg
    30661 ttttcgacgc gccacaactc accacggttg ccaaagagtg gccgctgttt tcgtggtgat
    30721 acgaccaaaa ttcaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga aatccggtct
    30781 tatttgctgc ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc accggtagca
    30841 acatcacgct gtttgccagt gaagaacggg tggcacttgc tgcacacgtc gaggttcagg
    30901 tcatgaccaa cggtggagcg gatttttatt acgttaccgc aagagcagct agcagtaatt
    30961 tcttcgtatt tcgggtgaat atcttttttc atgggaaaac ctcggtttaa ggccgcgtcg
    31021 ctcttccagc cctaacgcca gacaccacgc gatgttaaaa gtatagcttc aatacgatca
    31081 tttcgtacga aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac tgatccgcac
    31141 tcttctacgg caatgtgtat actaacccac cgaatttcaa gtcaggatga tgctatgccc
    31201 gttgcccacg ttgccttgcc cgttccgctt cctcgtacct ttgactatct gctgccagaa
    31261 ggcatgacgg ttaaagctgg gtgtcgcgtg cgcgtgccgt ttggcaaaca gcaggagcgc
    31321 atcgggattg tggtatcagt tagcgatgcc agcgaactgc cgctcactga gctaaaagcg
    31381 gtagtcgaag tgctggatgg tgagccggtg tttactcact ccgtctggcg attgctgcta
    31441 tgggcggcag attactatca tcatccgatt ggcgatgtgc tgtttcatgc cttgccgatt
    31501 ttactacgcc aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt tgccactgaa
    31561 caaggtcagg cggtggatct gaacagcctg aaacgctcac ccaagcaaca acaggcgctg
    31621 gcagcgttac ggcaaggcaa aatctggcgc gaccaggttg ccacgctcga atttaatgat
    31681 gccgcgttgc aggcgctgcg caaaaaaggt ctgtgtgatt tagcaagtga aacaccagag
    31741 tttagcgact ggcgaacgaa ctatgccgtt tctggtgagc ggttgcgatt gaataccgaa
    31801 caggccaccg ccgttggcgc aattcatagc gcggcagata ctttttctgc ctggctgctg
    31861 gcgggcgtta ccggttccgg taaaacggag gtttatctca gtgtactgga aaacgtgctc
    31921 gctcagggca aacaggcgct ggtgatggtg ccggaaatcg gcctgacacc gcaaactatc
    31981 gcccgttttc gtgaacggtt taatgcgccc gtggaagttc tgcattccgg tctgaacgac
    32041 agcgagcgtc tttcggcgtg gctgaaagcg aaaaatggcg aggcggcgat tgtgatcggc
    32101 acccgctccg cgctgtttac gccgtttaaa aatctcggcg tgattgtcat tgacgaagag
    32161 cacgacagtt cctacaagca gcaggaaggc tggcgctatc atgcccgcga cctggcggtg
    32221 tatcgtgcgc atagcgagca aatcccgatt attcttggct ccgcaacgcc cgcgctggaa
    32281 acgttgtgca acgtccagca gaaaaaatac cgcctgctgc gcctgacgcg ccgggcgggg
    32341 aatgcgcgtc cggcaattca acacgtgctg gatttaaaag gtcagaaggt gcaggcaggt
    32401 ctggctccgg cgttaatcac ccgtatgcgc cagcatttac aggccaacaa tcaggttatc
    32461 ctctttctta accgccgtgg ctttgcgcct gcgctactgt gccacgactg cggctggatt
    32521 gccgaatgcc cacgttgcga tcactactac acgctgcatc aggcgcaaca gcatttgcgc
    32581 tgtcaccact gtgacagtca gcgtccggtg ccgcgccagt gcccttcctg cggttccacg
    32641 cacctggtac ccgtggggct gggcaccgaa cagcttgaac agacgcttgc gccgctgttc
    32701 cctgatgtgc ccatttctcg tatcgaccgc gataccacca gccgcaaagg ggcgctggaa
    32761 cagcaactgg cggaagtaca tcgcggcggt gcgaggattt tgatcggcac acaaatgctg
    32821 gcgaaaggtc accactttcc ggatgtgacg ctggtggcgt tactggatgt ggacggcgcg
    32881 ctgttttctg ccgattttcg ctcggcagag cgtttcgctc agctttacac ccaggtcgct
    32941 ggtcgtgccg ggcgtgcggg taaacaaggc gaagtggtgc tgcaaacgca ccatccggaa
    33001 catcctctgt tgcaaacgtt gctctataaa ggctacgatg cctttgccga acaggcgctg
    33061 gctgagcgac ggatgatgca actgccgcca tggaccagcc atgtgattgt gcgtgcggaa
    33121 gatcataaca atcagcatgc gccgctgttc ctgcaacaac tgcgtaatct gatcctctcc
    33181 agcccgctgg cagacgacaa actgtgggtt ctcggtccgg ttccggcact ggcacctaaa
    33241 cgtggcggtc gctggcgctg gcagatattg ttgcagcacc cttcccgcgt gcgcttgcaa
    33301 cacatcatta gtggtacgct ggcgctcatc aatacaatac cggattcccg caaggtgaaa
    33361 tgggtactgg atgttgatcc gattgagggt taaaccgctt acgatgcgag gcggatcgaa
    33421 aaattcaata ttcatcacac ttttcatgaa aattctgtaa ccgttttcac gcgctatctg
    33481 ctaaaaatgt tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt gccaggcgag
    33541 gagtgagtgt gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac gttgccctca
    33601 aggcaaaagt ctctacagcg actgtctccc gagcattaat gaatcccgat aaagtctccc
    33661 aggccacccg taatcgggtt gaaaaagcgg cccgggaagt gggttattta ccgcagccta
    33721 tggggcgcaa cgtcaagcgt aatgaatccc gcaccattct ggtgattgtc ccggatatct
    33781 gcgatccctt ctttagcgaa attattcgcg gtatcgaagt tacggcggca aatcacggat
    33841 atctggtgct gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc tttatcgatt
    33901 tgatcatcac caagcaaatt gatggcatgt tgctgctggg ttcaaggctg ccgtttgatg
    33961 ccagcattga ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa tttgcaccgg
    34021 aactggagct gcctaccgtt catatcgaca atctgaccgc cgcatttgat gcagtaaatt
    34081 atttatatga gcaagggcat aaacggattg gctgtatagc cggtcccgaa gagatgccac
    34141 tgtgtcacta ccgcctgcaa ggctatgttc aggcgctgcg tcgctgcgga attatggttg
    34201 atccacaata catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa gcaatgcaac
    34261 agctgcttga tcttccacaa ccgcctactg cagtcttctg ccatagcgat gtgatggcgc
    34321 tcggcgcact ttctcaggca aaacgccagg ggctgaaagt cccggaagac ctttccataa
    34381 tcggttttga taacatcgac ctgacgcaat tttgtgatcc gccgctgaca accatcgcgc
    34441 agccgcgtta cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa atgcaggggc
    34501 aacacgttgg cagtggctct cgtttaatgg actgcgaact tatcatccgg ggatcaacac
    34561 gcgcgttacc ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc ccgccgctta
    34621 agtaacatgg cgggctgacg aacgaataaa tacagcgaaa cgatagtggc acaacgagat
    34681 tatgtacgcc gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc acggaaaaag
    34741 caacgaaatc tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc cgttcttgtg
    34801 acctttatcg gtggtctgta cttcattacg catcacaaga aagaagagtc cgagacgctg
    34861 caaagccaga aagtgaccgg aaacggacta ccaccaaaac cagaagaacg ctggcgctac
    34921 attaaagagc tggaaagtcg ccagccgggt gtgcgtgcgc ccacagaacc ttctgccggt
    34981 ggtgaagtga aaacgccgga gcaactgaca ccagaacaac gtcagcttct tgaacaaatg
    35041 caggctgata tgcgccagca gccaacgcag ctggttgagg ttccgtggaa tgaacagacg
    35101 ccggaacagc gtcagcaaac gctacaacgc cagcgtcagg cgcagcaatt agcggagcag
    35161 caacgcctgg ctcagcagtc gcgtacgact gaacaaagct ggcagcagca gacgcgtacg
    35221 tcgcaagccg cgccggtaca ggcgcaaccg cgccagtcta aaccagcttc tactcagcag
    35281 ccgtaccagg atctgctgca aacccctgcg cacacgaccg cgcagtcgaa gccgcagcaa
    35341 gctgcgccgg ttgctcgtgc cgctgacgcg ccaaaaccga cggcggagaa aaaagacgaa
    35401 cgccgctgga tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga gacggtacgt
    35461 gctcagctgg cgttcgaagg atttgactcg aaaatcacca ccaacaatgg ctggaatcgt
    35521 gtggtcattg gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa tcggttgaag
    35581 atggcgggtc atacaaactg cattcggctc gccgccgggg gttgaaaccc tcaaaatccc
    35641 ccccatctat aattgcatta tgccccgtac ttttgtacgg ggtttgtact ctgtattcgt
    35701 aaccaagggg tcagctcgtg acaactatag taagcgtacg ccgtaacggc catgtggtca
    35761 tcgctggtga tggtcaggcc acgttgggca ataccgtaat gaaaggcaac gtgaaaaagg
    35821 tccgccgtct gtacaacgac aaagtcatcg cgggctttgc gggcggtact gcggatgctt
    35881 ttacgctgtt cgaactgttt gaacgtaaac tggaaatgca tcagggccat ctggttaaag
    35941 ccgccgttga gctggcaaaa gactggcgta ccgatcgcat gctgcgcaaa cttgaagcac
    36001 tgctggcagt cgcggatgaa actgcatcgc ttatcatcac cggtaacggt gacgtggtgc
    36061 agccagaaaa cgatcttatt gctatcggct ccggcggccc ttacgcccag gctgcggcgc
    36121 gcgcgctgtt agaaaacact gaacttagcg cccgtgaaat tgctgaaaag gcgttggata
    36181 ttgcaggcga catttgcatc tataccaacc atttccacac catcgaagaa ttaagctaca
    36241 aagcgtaagg atctcccatg tctgaaatga ccccacgcga aatcgtcagc gaactggata
    36301 agcacatcat cggccaggac aacgccaagc gttctgtggc gattgctctg cgtaaccgct
    36361 ggcgtcgcat gcagctcaac gaagagctgc gccatgaagt gaccccgaaa aatatcctga
    36421 tgatcggccc gaccggtgtc ggtaaaactg aaatcgcccg tcgtctggct aagctggcga
    36481 atgcgccgtt catcaaagtt gaagcgacca aatttaccga agtgggctac gtcggtaagg
    36541 aagttgattc tattattcgc gatctgaccg atgccgccgt gaaaatggta cgcgtccagg
    36601 ctatcgagaa aaaccgttat cgcgctgaag aactggcaga agaacgtatt ctcgacgtgc
    36661 tgatcccacc tgctaaaaac aactggggac agactgaaca gcagcaggaa ccttccgctg
    36721 ctcgtcaggc attccgcaaa aaactgcgtg aaggccagct tgatgacaaa gaaatcgaga
    36781 tcgatcttgc cgcagcaccg atgggcgttg aaattatggc tcctccgggc atggaagaga
    36841 tgaccagcca gctgcagtcc atgttccaga acctgggcgg ccagaagcaa aaagcgcgta
    36901 agctgaaaat caaagacgcc atgaagctgc tgattgaaga agaagcggcg aaactggtga
    36961 acccggaaga gctgaagcaa gacgctatcg acgctgttga gcagcacggg atcgtgttta
    37021 tcgacgaaat cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat gtttctcgtg
    37081 aaggcgttca gcgtgacctg ctgccgctgg tagaaggttg caccgtttcc accaaacacg
    37141 ggatggtcaa aactgaccac attctgttta tcgcttctgg cgcgttccag attgcgaaac
    37201 cgtctgacct gatcccggaa ctgcaaggtc ggctgccaat ccgcgttgaa ctgcaggcac
    37261 tgaccaccag cgacttcgag cgtattctga ccgagccgaa tgcctctatc accgtgcagt
    37321 acaaagcact gatggcgact gaaggcgtaa atatcgagtt taccgactcc ggtattaaac
    37381 gcatcgcgga agcggcatgg caggtgaacg aatctaccga aaacatcggt gctcgtcgtt
    37441 tacacactgt tctggagcgt ttaatggaag agatttccta cgacgccagc gatttaagcg
    37501 gtcaaaatat cactattgac gcagattatg tgagcaaaca tctggatgcg ttggtggcag
    37561 atgaagatct gagccgtttt atcctataat cgcgttcaat cattttcatc attgtttgat
    37621 ggggctgaaa ggccccattt ttattggcgc gtattatgac tgaacaacaa attagccgaa
    37681 ctcaggcgtg gctggaaagt ttacgaccta aaaccctccc cctcgccttt gctgcaatta
    37741 tcgtcgggac ggcgctggca tggtggcaag gtcacttcga tccgctggtc gccctgctgg
    37801 cactaattac cgccgggcta ttacagatcc tttctaacct cgccaatgat tacggcgatg
    37861 cggtaaaagg cagcgataaa cctgaccgca ttgggccgct acgcggcatg caaaaagggg
    37921 tcattaccca gcaaaagatg aaacgggcgc tcattattac cgtcgtgctc atctgtctct
    37981 ccgggctggc actggttgca gtggcatgcc atacgctggc cgattttgtc ggtttcctga
    38041 ttcttggcgg gttgtcgatc attgccgcta tcacctacac cgtgggcaat cgtccttatg
    38101 gttatatcgg tctgggtgat atttccgtac tggttttctt tggctggttg agtgtcatgg
    38161 ggagctggta tttacaggct catacattga ttccggcact gatccttccg gcgaccgcat
    38221 gcggcctgct ggcaacggca gtactgaata ttaataacct gcgtgatatc aatagcgacc
    38281 gcgaaaatgg caaaaacacg ctggtggtgc gcttaggtga agtgaacgcg cgtcgttatc
    38341 atgcctgcct gctgatgggc tcgctggtgt gtctggcgct gtttaatctc ttttcgctgc
    38401 atagcctgtg gggctggctg ttcctgctgg cggcaccatt actggtgaag caagcccgtt
    38461 atgtgatgcg ggaaatggac ccggtggcga tgcgaccaat gctggaacgt actgtcaagg
    38521 gagcgttact gactaacctg ctgtttgttt tagggatatt cctaagccag tgggcagcat
    38581 aactgacaaa tatcaattaa caattgatga ttttgccaac agcccacata gcgcgatata
    38641 ctgaaaattc tcgcagcaac tgaatgttaa gcctatgaaa tacgatactt ccgagctttg
    38701 tgacatctat caagaagatg ttaacgtcgt ggaaccgctg ttctccaact ttggcggacg
    38761 ggcgtcgttt ggcggacaaa taatcacggt aaaatgtttc gaggacaacg ggttgctgta
    38821 cgatctgctc gaacagaatg gccgtggtcg tgttcttgtc gtcgatggcg gtggttctgt
    38881 tcgtcgcgca ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg aatgggaagg
    38941 tctggtcatt tacggcgcgg tgcgtcaggt agatgacctg gaagagttgg atatcggcat
    39001 ccaggcgatg gcggcaattc cggttggtgc cgctggcgaa ggcattggcg aaagcgatgt
    39061 ccgcgtcaat tttggcggtg tcaccttctt ctccggcgac catctttatg ccgacaatac
    39121 cgggattatt ctttcagaag atccgctgga tattgaatga taagaaaggc accgcaaggt
    39181 gccttttttc tgcgttacct gttggcctac acagtaaaga aattacgcga aagatgaagc
    39241 gtaatcagac ctcttccatg cgacccagca gggcctgcag acgttcctgc cagccgttct
    39301 gctgttcttt cagatggttg ttctcacgct ccagctcttc gcgctgatgc tgggcatttt
    39361 gaacttcctg cgacagtgag ttgttttttt ctttcagctc ttcgatttcc atctgcaaca
    39421 gagtgatggt atcaatcgcc tgctgtactt ttgcttccag tttctcaaac acttctaatg
    39481 acattgtcat acctctcctg aattgcaagg cgttgatgga taaaaaatcc tcgtcccgat
    39541 taccggtgac gccttaataa atacgagcgc actttagtta gctccgattg tatgaagccg
    39601 cgccatcgct gtccagcggc acgccttgca gattacggtt tgccacactt ttcatccttc
    39661 tcctagtgac ataatccaca tcaatcgaaa atgttaataa atttgttgcg cgaatgatct
    39721 aacaaacatg catcatgtac aatcagatgg aataaatggc gcgataacgc tcattttatg
    39781 acgaagcaca cacattttaa gttcgatatt tctcgttttt gctcgttaac gataagttaa
    39841 cagcatgcct acaagcatcg tggaggtccg tgactttcac gcatacaaca aacattaact
    39901 cttcaggatc cgattatgag tcaaacatca accttgaaag gccagtgcat tgctgaattc
    39961 ctcggtaccg ggttgttgat ttttttcggt gtgggttgcg ttgcagcact aaaagtcgct
    40021 ggtgcgtctt ttggtcagtg ggaaatcagt gtcatttggg gactgggggt ggcaatggcc
    40081 atctacctga ccgcaggggt ttccggcgcg catcttaatc ccgctgttac cattgcattg
    40141 tggctgtttg cctgtttcga caagcgcaaa gttattcctt ttatcgtttc acaagttgcc
    40201 ggcgctttct gcgctgcggc tttagtttac gggctttact acaatttatt tttcgacttc
    40261 gagcagactc atcacattgt tcgcggcagc gttgaaagtg ttgatctggc tggcactttc
    40321 tctacttacc ctaatcctca tatcaatttt gtgcaggctt tcgcagttga gatggtgatt
    40381 accgctattc tgatggggct gatcctggcg ttaacggacg atggcaacgg tgtaccacgc
    40441 ggccctttgg ctcccttgct gattggtcta ctgattgcgg tcattggcgc atctatgggc
    40501 ccattgacag gttttgccat gaacccagcg cgtgacttcg gtccgaaagt ctttgcctag
    40561 ctggcgggct ggggcaatgt cgcctttacc ggcggcagag acattcctta cttcctggtg
    40621 ccgcttttca gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg caaactgatt
    40681 ggtcgccatt tgccttgcga tatctgtgtt gtggaagaaa aggaaaccac aactccttca
    40741 gaacaaaaag cttcgctgta atatgactac gggacaatta aacatgactg aaaaaaatat
    40801 atcgttgcgc tcgaccaggg caccaccagc tcccgcgcgg tcgtaatgga tcacgatgcc
    40861 aatatcatta gcgtgtcgca gcgcgaattt gagcaaatct acccaaaacc aggttgggta
    40921 gaacacgacc caatggaaat ctgggccacc caaagctcca cgctggtaga agtgctggcg
    40981 aaagccgata tcagttccga tcaaattgca gctatcggta ttacgaacca gcgtgaaacc
    41041 actattgtct gggaaaaaga aaccggcaag cctatctata acgccattgt ctggcagtgc
    41101 cgtcgtaccg cagaaatctg cgagcattta aaacgtgacg gtttagaaga ttatatccgc
    41161 agcaataccg gtctggtgat tgacccgtac ttttctggca ccaaagtgaa gtggatcctc
    41221 gaccatgtgg aaggctctca cgagcgtgca cgtcgtggtg aattgctgtt tggtacggtt
    41281 gatacgtggc ttatctggaa aatgactcag ggccgtgtcc atgtgaccga ttacaccaac
    41341 gcctctcgta ccatgttgtt caacatccat accctggact gggacgacaa aatgctggaa
    41401 gtgctggata ttccgcgcga gatgctgcca gaagtgcgtc gttcttccga agtatacggt
    41461 cagactaaca ttggcggcaa aggcggcacg cgtattccaa tctccgggat cgccggtgac
    41521 cagcaggccg cgctgtttgg tcagttgtgc gtgaaagaag ggatggcgaa gaacacctat
    41581 ggcactggct gctttatgct gatgaacact ggcgagaaag cggtgaaatc agaaaacggc
    41641 ctgctgacca ccatcgcctg cggcccgact ggcgaagtga actatgcgtt ggaaggtgcg
    41701 gtgtttatgg caggcgcatc cattcagtgg ctgcgcgatg agatgaagtt gattaacgac
    41761 gcctacgatt ccgaatattt cgccaccaaa gtgcaaaaca ccaatggtgt gtatgtggtt
    41821 ccggcattta ccgggctggg tgcgccgtac tgggacccgt atgcgcgcgg ggcgattttc
    41881 ggtctgactc gtggggtgaa cgctaaccac attatacgcg cgacgctgga gtctattgct
    41941 tatcagacgc gtgacgtgct ggaagcgatg caggccgact ctggtatccg tctgcacgcc
    42001 ctgcgcgtgg atggtggcgc agtagcaaac aatttcctga tgcagttcca gtccgatatt
    42061 ctcggcaccc gcgttgagcg cccggaagtg cgcgaagtca ccgcattggg tgcggcctat
    42121 ctcgcaggcc tggcggttgg cttctggcag aacctcgacg agctgcaaga gaaagcggtg
    42181 attgagcgcg agttccgtcc aggcatcgaa accactgagc gtaattaccg ttacgcaggc
    42241 tggaaaaaag cggtcaaacg cgcgatggcg tgggaagaac acgacgagta atgtcaggca
    42301 cctgaatcga ttgcctgatg ctgatcgcct gatgcgacgc ttacgcgtct tatcaggcct
    42361 acgagccaat gccattttgt gtaggcggga taaggcgttc acgccgcatc cggcatcacg
    42421 cagactctga aaccgtgcgg gggcaacccc gcacacatca ataatcccac ccttcccctg
    42481 tgctacactt cgcgccattc cttactgctt agagtttgct atgagacgag aacttgccat
    42541 cgaattttcc cgcgtcaccg aatctgcggc gctggctggc tacaaatggt taggacgcgg
    42601 cgataaaaac accgcggacg gcgcggcggt aaacgccatg cgtattatgc tcaaccaggt
    42661 caacattgac ggcaccatcg tcattggtga aggtgaaatc gacgaagcac cgatgctcta
    42721 cattggtgaa aaagtcggta ctggtcgcgg cgacgcggta gatattgctg ttgatccgat
    42781 tgaaggcacg cgcatgacgg cgatgggcca ggctaacgcg ctggcggtgc tggcagtagg
    42841 cgataaaggc tgcttcctca atgcgccgga tatgtatatg gagaagctga ttgtcgggcc
    42901 gggagccaaa ggcaccattg atctgaacct gccgctggcg gataacctgc gcaatgtagc
    42961 ggcggcgctc ggcaaaccgt tgagcgaact gacggtaacg attctggcta aaccacgcca
    43021 cgatgccgtt atcgctgaaa tgcagcaact cggcgtacgc gtatttgcta ttccggacgg
    43081 cgacgttgcg gcctcaattc tcacctgtat gccagacagc gaagttgacg tgctgtacgg
    43141 tattggtggc gcgccggaag gcgtggtttc tgcggcggtg atccgcgcat tagatggcga
    43201 catgaacggt cgtctgctgg cgcgtcatga cgtcaaaggc gacaacgaag agaatcgtcg
    43261 cattggcgag caggagctgg cacgctgcaa agcgatgggc atcgaagccg gtaaagtatt
    43321 gcgtctgggc gatatggcgc gcagcgataa cgtcatcttc tctgccaccg gtattaccaa
    43381 aggcgatctg ctggaaggta ttagccgcaa aggcaatatc gcgactaccg aaacgctgct
    43441 gatccgcggc aagtcacgca ccattcgccg cattcagtcc atccactatc tggatcgcaa
    43501 agacccggaa atgcaggtgc acattctctg agtgatttga tcgattgagc cttccagtcc
    43561 ttcgggactg gaattttttt gttcggagaa cgaagataag gcaagtcaat caaaacagga
    43621 gaaaaacatg gctgattggg taacaggcaa agtcactaaa gtgcagaact ggaccgacgc
    43681 cctgtttagt ctcaccgttc acgcccccgt gcttccgttt accgccgggc aatttaccaa
    43741 gcttggcctt gaaatcgacg gcgaacgcgt ccagcgcgcc tactcctatg ttaactcgcc
    43801 cgataatccc gatctggagt tttacctggt caccgtcccc gatggcaaat taagcccacg
    43861 attggcggca ctgaaaccgg gcgatgaagt gcaggtggtt agcgaagcag ctggcttctt
    43921 tgttctggat gaagtaccgg actgcgaaac gctatggatg ctggcaaccg gtacagcgat
    43981 tggcccttat ttatcgattc tgcaactagg caaagattta gatcgcttca aaaatctggt
    44041 cctggttcac gccgcacgtt atgccgccga cttaagctat ttgccactga tgcaagaact
    44101 ggaaaaacgc tacgaaggca aactgcgcat tcagacggtg gtcagtcggg aaacggcagc
    44161 ggggtcgctc accggacgga taccggcatt aattgaaagt ggggaactgg aaagcgcgat
    44221 tggcctgccg atgaataaag aaaccagcca tgtgatgctg tgcggcaatc cacagatggt
    44281 gcgcgataca caacagttgc tgaaagagac ccggcagatg acgaaacatt tacgtcgccg
    44341 accgggccat atgacagcgg agcattactg gtaagcggtt acttatcgat aaacggcacg
    44401 atgagcaaat ccgcactcat cttattgatc atcccgcgat atgccggcat caaacggttg
    44461 ataaatgagt gatgatgacc acagacaagg aggtcgcact gctctttttg cataatttcc
    44521 agcagtgttt ccggcatttc tccgcgttca atacgcagtt ttgtcttcgg ccattgaata
    44581 tttttcgtca gtttatacag cttgttatcc gacttattct tcaacaattg aagaatatct
    44641 tctgttgcag ggaagtagat acccggatac aactcgctta agccatcatc aatatgaatt
    44701 aacgtcaggt gagcgtcatt atgtctggcg agctccaggg ctttattcac cagtaaggca
    44761 tcttcttcat tcccggaaat tgccacgcca atgtgtttat aagccatgtt taactccttc
    44821 taaagcaact ccatcaagct aacgaacgca gtgataactc aaaaataatc atctcggcct
    44881 ggcatgagaa agtgaaggcc gcatcaagct caacttgccc ctccagatcc ttaatcttat
    44941 aggccacgct ggccccttca tctgcagagg atgcagcggc ggcaagctct ttcagtttgg
    45001 tcataaaaga ctctgcttct tcgcgggtgg caaaaaagcg cgaaaactta ctggtacagt
    45061 tgtcgttatc aatcaccgta ccgatatcaa tcgcacatcc tttagtactg catttatcta
    45121 cgacatcttt catagggcac ctctgtatat tcgcctctct gtcgattacg acttaagaat
    45181 aatgcgccgt taagggaaga acaaacaagt aatgatctta tggattacca ttttttttga
    45241 tattgcttaa ggtgaagagt tcagaaggat tttccgggta ttgtcatttg catcgtggaa
    45301 ataacaatcc cggaaaaaaa tagcagtaag agatatatta aaggttaatt acttgattta
    45361 ttgtcggctt tatacttcac atcctgagta tctttaccat atttattttc gccttgtgtg
    45421 ccaacaaacg caccaagatc gataagcatc atcaccagaa tcaacgtcgg gacaaaacgc
    45481 cccaccgccc attgccagac acccggtaaa atcgctcagt tacccgccag cagcatccac
    45541 gccacaatca tcagaaatgc ccatgcgccg gaacgcccgc gatcatgcaa gcgcttaaca
    45601 gttactgccg ctgttggcca gagcaagcac acaaggcaaa acgccgcggt ctgaatatcg
    45661 agtagattct tacccgccag tgagaaaagc accagcatgc ctgcgaacca caggcctatc
    45721 caaatccaga aatcacggcg tccaatacgc cctttaaatg agaataacca ttgctgtatg
    45781 gtcatgtaag ttccttgatg gttgtctttt ccaggattct acccttttga caagggcgac
    45841 agatatcgtt ttaatcggag ccagtcataa caaaaggtac tgtcaatgaa gccagggtgt
    45901 acgctgtttt ttctcttatg ttctgcatta accgttacaa caacggcgca tgcacaaaca
    45961 ccagatacgg caacgaccgc gccttatctg ctggctggag cccctacttt cgatctctcc
    46021 atcagccagt ttcgagaaga ctttaacagc cagaatccca gcctgccact gaacgaattt
    46081 cgtgccatcg acagcagtcc cgacaaagcc aatctcactc gtgctgcaag taaaattaac
    46141 gagaacttgt atgcttctac agcgctggag cgcggtacct taaaaatcaa aagcattcaa
    46201 atgacctggc tacccatcca ggggccagag caaaaagcag cgaaagcgaa agctcaggaa
    46261 tacatggcag cagtgatccg cacactcacc ccattaatga ccaaaacaca aagccagaaa
    46321 aaactgcagt cgctactaac ggcggggaaa aacaaacgtt attacaccga gacagaaggt
    46381 gcactgcgtt atgttgtcgc ggacaacggc gaaaaggggc tgaccttcgc tgttgaaccg
    46441 attaagctgg cgctatctga atcgcttgaa ggtttgaata aatgacaaaa agcaaagcct
    46501 ttgtgccgat gaatctctat actgtttcac agacctgctg ccctgcgggg cggccatctt
    46561 cctttattcg cttataagcg tggagaatta aaatgcgaca tcctttagtg atgggtaact
    46621 ggaaactgaa cggcagccgc cacatggttc acgagctggt ttctaacctg cgtaaagagc
    46681 tggcaggtgt tgctggctgt gcggttgcaa tcgcaccacc ggaaatgtac atcgatatgg
    46741 cgaagcgcga agctgaaggc agccacatca tgctgggtgc gcaaaacgtg gacctgaacc
    46801 tgtccggcgc attcaccggt gaaacttctg ccgctatgct gaaagacatc ggcgctcagt
    46861 acatcatcat cggccactct gaacgtcgta cttaccacaa agagtctgac gaactgattg
    46921 cgaaaaaatt cgcggtgctg aaagagcagg gtctgactcc ggttctgtgc atcggtgaaa
    46981 ccgaagctga aaacgaagcg ggaaaaactg aagaagtttg cgcacgtcag atcgacgcgg
    47041 tactgaaaac tcagggtgct gcggcattcg aaggtgcggt tatcgcttac gaacctgtat
    47101 gggcaatcgg tactggcaaa tctgcaactc cggctcaggc acaggctgtt cacaaattca
    47161 tccgtgacca catcgctaaa gttgacgcta acatcgctga acaagtgatc attcagtacg
    47221 gcggctctgt aaacgcgtct aacgctgcag aactgtttgc tcagccagac atcgacggcg
    47281 cgctggttgg cggtgcttct ctgaaagctg acgctttcgc agtgatcgtt aaagctgcag
    47341 aagcggctaa acaggcttaa gtctgacagg tgccggattt catatccggc acttactttc
    47401 cttaactcct cgccttaacg caaaatctca cactcatgat cctggatttc ctcggctgaa
    47461 gcacggttca gcgtcagtag atttcgttgt gtcgccagca acacaaacga attatcactc
    47521 tgccgtacca tcgccagccc gtagcttccc atatgttccc gcgcctcagg cacttcttct
    47581 gcaagcatca taaatgggct gcgttgtacc agttcgcttt ccgttacccg acgcgccagg
    47641 tattcatgcc cgcgcaaacc acctggcagt ggcaaccagc ggctgctgat gttcgccaga
    47701 tttttatcca gttgttcgcg cacatcagga cgaatacaag agatatgaat atgaaaatgg
    47761 ttttgcgtat gcccggtgcg ggagttgatc gccaaagaaa ccgcgcgatc gggaaccggc
    47821 tggccgtatt ttttgctcat aaaatcacgc gcctgccagg ccaaccaaaa gaagttcggc
    47881 gttgaaggat cggtcaacaa aggactttca gtaccgttaa tacgatacgt tggcatcaac
    47941 agatattgca gtggaccatt aagatctttt aaaaccacgt atccggcatt gggtttgact
    48001 tccgcacatg gcgaaggatt ttgattttgc tgctgattgg gcaaacattc ctcaaggaca
    48061 atcttacgta atgtatccga ctcttcaccg gttaatttcc agtaaccaat accggcagcc
    48121 acaacggcga taactatcat caccaaaaaa agaagacccg cttttttcat ctttttttcc
    48181 ctgtacctca aagagatgca agggtaacgc aaaatcgtga caaataaaaa acccggtcgc
    48241 gagaaaccac cgggtttgat aattatccta gggaaatcag cgtttgctga tctgatcgaa
    48301 cgtaccgccg ttagcaaaat gctctttttg cgctttcgtc cagccgccga actcttcatc
    48361 aatggtgaat aacttcagct ttggaaacgc attttcgtac tttttcgcta cttcagcgtc
    48421 gcgcggacgg tagtagtttt tcgcggcaat ttcctgacct tctggcgagt agagatattt
    48481 caggtaggct tccgccacct ctttggtgcc ttttttctcg accactttat cgaccaccga
    48541 caccgttggc tctgcgagga tagactcact cggcgtgacg atttcgaatt tatctttccc
    48601 cagttcattc gctgccagca gagcttcgtt ttcccaggcg atcagtacat cgccaatccc
    48661 gcgctcgaca aaggtgttag tggagccgcg cgcgccagaa tccagaactt cgacgttttt
    48721 atacagtgcc cgaacaaaat cctgtgcttt tgcctgatcg ttgttgttgt gatgcagcgc
    48781 gtagccccag gcagccaggt agttccagcg tgcgccacca gagcttttcg gattaggcgt
    48841 gatcaccgaa acacccggtt taatcagatc gttccagtca tggatctgct tcggattgcc
    48901 cttacgcacc aggaaaacaa tggtggaagt gtacggtgcg gagttatccg gcagacgttt
    48961 gatccactct ttatcaatcc gcccgcgttc cgcaatcgcg tccacgtcat aggccagagc
    49021 cagcgtgaca acatcagctt caataccgtt gattaccgac gtcgcttgtt tacctgaacc
    49081 accgtgtgac tgacgaatca ccacgttatc accagtttgc tgtttccagt gggcgctgaa
    49141 tgccttgttg tactgttcgt acaattcgcg cgttggatca tatgaaacgt taagaagctg
    49201 aatatccttt gccataacgc tggttgccgc cagcaaaaat gttaacccta cgccccactt
    49261 gttcatcgcc cgactctctt atgttgtgtt gtgatgagca aagcgtgcca gaaggttaac
    49321 caaacattaa agaataaaaa aagattggct ataacttgcg ggtatatgtt gagggattaa
    49381 aaaggcggaa gaaatccgcc tcatattgct gacaaagtgc gctttgtcca tgccggatgc
    49441 ggcgcgaacg ccttatccgg cctacaaaag tttgcaaatt caataaattg cagaattcat
    49501 gtaggcctga taagcgaagc gcatcaggca tttttgcttc tgtcatcggt ttcaggctaa
    49561 aggaatctgc ctttttccga aatcattaat acagtttttt cgcgcagtcc agccagtcac
    49621 ctttgaacgg acgcttcatg ttttcgatag cgtcgatgat gtcgtggtga accagctgtt
    49681 cgttctggat acctacacaa cgaccgccgt aacctgccag cagcagatcg atagcgtaag
    49741 cgcccatacg ggaagccaga atacggtcgt aaggcaccgg agaaccaccg cgctggatgt
    49801 ggcccagcac agttgcgcgg gtttcacgac cggtttcttt ctcgatgaaa tgcgccagtt
    49861 cgtcaacatc acacatatgt tcggtaatcg ccacgatcgc gtgtttttta cctttcgcga
    49921 tacccgcttt gatttcgttt accaggtctt cacggctgaa ttcaacttcc ggaactacaa
    49981 cgaattcaca gccaccggca atggccgcag ccagggtcag atcgccacaa taacggccca
    50041 tcacttccac cacggaaata cgctggtgag aagaagaggt gtcacgcaga cggtcgatcg
    50101 cttctacaac ggtgctcagc gcagtgaaga aaccgatagt gtagtcagtg cctttgatgt
    50161 cgttgtcgat agtgcccggc agaccgatgc acgggaagcc catttcggtc agacgcattg
    50221 cacccatgta ggaaccgtca ccgccgataa ccaccagcgc gtcgatacca cgttttttca
    50281 ggttttcgat agccacggcg cggatgttct cgtcgcggaa ttccgggaaa cgcgcagaac
    50341 cgaggaacgt accgccacgg ttgatcatgt cagacacgct gtaacggtct agctgtacca
    50401 tacggtcttc atacagaccc agatagccgt cataaatacc cattacttcc agaccttctg
    50461 tcagcgcaga acgaacaacc ccgcgaattg cggcgttcat gcctggcgca tcaccgccgc
    50521 ttgtcaacac acctattttc ttaatcatga ctacctctga actttggaat gcaaaatgaa
    50581 atctgttgcc ggaagtcttc ttgcacatcg aagtgatcca acgaatgtgc aaatagtata
    50641 acaatcactt cctgctgaat tgattcaggt caggccaaat ggcggtattt tatacacaaa
    50701 atgcgggtct ggctctcttt tatactgatt atgaaagcat agaccgttta ccctccctgg
    50761 gtacgacgga acaggggtcc tgatggataa tcacatccga tcccggaaaa cgccgtaaaa
    50821 tagcctgctc tacctgatcc gccaccatat gtgcctgaac caaaggcaga gagtcttcca
    50881 tttccaaatg aatctgaata aagcgggtcg gccctgactg ccgcgtgcga agatcgtgag
    50941 cgccgctaac acccggccag gaagtcacga tatcaataat ttcttgccgt tcttcatcag
    51001 gcaatgcgcg atccagtaat gactgtaccg cctcatatcc catgcgtaac gcgctatata
    51061 aaatatagat gccgattccc aatgcaaaca gagcatcggc gcgatgccag ccgtaccaag
    51121 acaaccccag cgccagcaga attgcgccgt tcatcataac atcagactgg taatgtagca
    51181 tatcagcccg caccgcctgg ctttgcgtcc ggcgcaccac ccaccgctga aacgagacaa
    51241 ggataatcgt acaaattagc gccacaattg tcacgataac cccgacgcct ggatctgtca
    51301 tcggtgttgg agataccaga tgttgaatac ccgtcaaaaa caggaatagt gccgaaccgg
    51361 agataaacat actttgcgcc agcgccgcga gggactcagc tttaccgtga ccaaacgagt
    51421 gattatcgtc ggcaggttgc agggaatatc gcaccaccag taaattcgtc aacgacgcgc
    51481 cgatatccac cagcgaatcc accagcgcgg cgagaatact caccgacccg gtataccacc
    51541 atgcaaaaat tttaatcagc aatagcaacg aagccatcgc cgtcgcagca atcgccgccc
    51601 gactgaccag ccgtccataa gattgattca taaatactcc cgctatcaac tgacgctagt
    51661 ataacggaag caaatcatct gcaatgcatt aagcagcagg caaattgagg ataaaaaaaa
    51721 ccccacatca tgtgggggaa gacagggatg gtgtctatgg caaggaaaac agggtttact
    51781 actgggaacg tgagttgcta ctactcaata gcttcaacga tgaacttttt tgccattgcg
    51841 tcacgtcacg caactgctcc attcgttgtt gatgtttctc gtttaaaacc gcttgctgct
    51901 ccggcgttaa caggcgatac atttggttgc ggacttttgc catctcaacc tgacgagcaa
    51961 tttgctcatt cgccattttt tctgcctgtg cgcgcacagc gttttcatca aaattttctg
    52021 cggtgacaag gcgatgcatt gtctccagtt cgctaacatt aacaggaggc tgttcgtgcc
    52081 gggcctgttg cataagatct cgcatctgct gacgctgatg ttcggttaaa cttatgccgt
    52141 cgaacatatg gctctgcgta ctgcgctgcg taagttcttc acccggatgc cagttatcgc
    52201 ctgaaccgac ttcagcagcg tggcttaatg aactgactgc cagcgttgag gccatgacgg
    52261 cagcggtaac tatgcgcatc atttgctccc aaaatctttc tgtcgcgatt caacgataga
    52321 gagtttacga ttcaggctgc aaacatgcgt cagggggtgt aaaacaacgt aaagtcatgg
    52381 attagcgacg tctgatgacg taatttctgc ctcggaggta tttaaacaat gaataaaatc
    52441 ctgttagttg atgatgaccg agagctgact tccctattaa aggagctgct cgagatggaa
    52501 ggcttcaacg tgattgttgc ccacgatggg gaacaggcgc ttgatcttct ggacgacagc
    52561 attgatttac ttttgcttga cgtaatgatg ccgaagaaaa atggtatcga cacattaaaa
    52621 gcacttcgcc agacacacca gacgcctgtc attatgttga cggcgcgcgg cagtgaactt
    52681 gatcgcgttc tcggccttga gctgggcgca gatgactatc tcccgaaacc gtttaatgat
    52741 cgtgagctgg tggcacgtat tcgcgcaatc ctgcgccgtt cgcactggag cgagcaacag
    52801 caaaacaacg acaacggttc accgacactg gaagttgatg ccttagtgct gaatccaggc
    52861 cgtcaggaag ccagcttcga cgggcaaacg ctggagttaa ccggtactga gtttaccctg
    52921 ctctatttgc tggcacagca tctgggtcag gtggtttccc gtgaacattt aagccaggaa
    52981 gtgttgggca aacgcctgac gcctttcgac cgcgctattg atatgcacat ttccaacctg
    53041 cgtcgtaaac tgccggatcg taaagatggt cacccgtggt ttaaaacctt gcgtggtcgc
    53101 ggctatctga tggtttctgc ttcatgatag gcagcttaac cgcgcgcatc ttcgccatct
    53161 tctggctgac gctggtgctg gtgttgatgt tggttttgat gttacccaag ctcgattcac
    53221 gccagatgac cgagcttctg gatagcgaac agcgtcaggg gctgatgatt gagcagcatg
    53281 tcgaagcgga gctggcgaac gatccgccca acgatttaat gtggtggcgg cgtctgttcc
    53341 gggcgattga taagtgggca ccgccaggac agcgtttgtt attggtgacc accgaaggcc
    53401 gcgtgatcgg cgctgaacgc agcgaaatgc agatcattcg taactttatt ggtcaggccg
    53461 ataacgccga tcatccgcag aagaaaaagt atggccgcgt ggaactggtc ggtccgttct
    53521 ccgtgcgtga tggcgaagat aattaccaac tttatctgat tcgtccggcc agtagttctc
    53581 aatccgattt cattaactta ttgtttgacc gcccgctatt actgctgatt gtcaccatgt
    53641 tggtcagtac gccgctgctg ttgtggttgg cctggagtct ggcaaaaccg gcgcgtaagc
    53701 tgaaaaacgc tgccgatgaa gttgcccagg gaaacttacg ccagcacccg gaactggaag
    53761 cggggccaca ggaattcctt gccgcaggtg ccagttttaa ccagatggtc accgcgctgg
    53821 agcgcatgat gacctctcag cagcgtctgc tttctgatat ctctcacgag ctgcgcaccc
    53881 cgctgacgcg tctgcaactg ggtacggcgt tactgcgccg tcgtagcggt gaaagcaagg
    53941 aactcgagcg tattgaaacc gaagcgcaac gtctggacag catgatcaac gatctgttgg
    54001 tgatgtcacg taatcagcaa aaaaacgcgc tggttagcga aaccatcaaa gccaaccagt
    54061 tgtggagtga agtgctggat aacgcggcgt tcgaagccga gcaaatgggc aagtcgttga
    54121 cagttaactt cccgcctggg ccgtggccgc tgtacggcaa tccgaacgcc ctggaaagtg
    54181 cgctggaaaa cattgttcgt aatgctctgc gttattccca tacgaagatt gaagtgggct
    54241 ttgcggtaga taaagacggt atcaccatta cggtggacga cgatggtcct ggcgttagcc
    54301 cggaagatcg cgaacagatt ttccgtccgt tctatcggac cgatgaagca cgcgatcgtg
    54361 aatctggcgg tacaggattg gggctggcga ttgttgaaac cgccattcag cagcatcgtg
    54421 gctgggtgaa ggcagaagac agcccgctgg gcggtttacg gctggtgatt tggttgccgc
    54481 tgtataagcg gagttaaact ccgcatttgt aggcaggata aggcgtttac gccgcatccg
    54541 gcatttgagc aggatgcctg atgcgacgct gatagcgtct tatcaggcct acactccggg
    54601 ggttattttc cccacaaccg gcgggaaaaa tcttcgatct tgccgctcag tcggcgcttt
    54661 tgcatcgtcc gcgtccagct tttcgataac ccggcggcgg agagtaaacg gtgatactga
    54721 tcgtcatcaa acggcatatg ccatgcgatg gcggcagcct cctgcacggt cacatcgctg
    54781 acacgggaaa ccaactccag cggcgcgtcc gcagacactt tccctggcgc tatcacgcta
    54841 tacaaccacc ccaccttacc tgtgttttgc atcaactgcg ctatatcgct gatatcaaaa
    54901 tggtaattga gtttatagca aggcgagcgc ggctggctga cctgaattaa tgcctctccc
    54961 cagcggaaaa tatcgcccat atgagcatta ctttccgtga gaccgtcggt tgagagattt
    55021 tcgccaaacg caggcgcaac aaacaactcc gcctgttccg gaaattcccg cacccagtag
    55081 agatagtgct cacgaggata atgacacagc gctctgtctg gcccgccgtg aacctttttc
    55141 tccgcctgct cgtcaccttc cagccccagc tccgtcagca tcaactcacc atcaacctgg
    55201 attttggcaa ttgcgctggg tttgccttcg ggataagcct gaatcttgcc tgtgtataca
    55261 tcaaccggat atcgcatctg ttccctctcc acgagaaatt tccccattag accatattta
    55321 ttttatgtca gccgtgctgt tttcgcggta agtatccagg caggaactca atagcaaacc
    55381 ggacgaacgt tagttcatcc ggctgtgggg
//
LOCUS       DAJTUI010000017        53217 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTUI010000017 DAJTUI010000000
VERSION     DAJTUI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53217
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.017"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(16..327)
                     /locus_tag="OO464_001099"
     CDS             complement(16..327)
                     /locus_tag="OO464_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR6226329.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            490..586
                     /gene="ffs"
                     /locus_tag="OO464_001100"
     ncRNA           490..586
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO464_001100"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            706..1059
                     /locus_tag="OO464_001101"
     CDS             706..1059
                     /locus_tag="OO464_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR6226330.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1101..2651)
                     /gene="pdeB"
                     /locus_tag="OO464_001102"
     CDS             complement(1101..2651)
                     /gene="pdeB"
                     /locus_tag="OO464_001102"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR6226331.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2815..3285)
                     /locus_tag="OO464_001103"
     CDS             complement(2815..3285)
                     /locus_tag="OO464_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR6226332.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3401..3952)
                     /gene="maa"
                     /locus_tag="OO464_001104"
                     /pseudo
     CDS             complement(3401..3952)
                     /gene="maa"
                     /locus_tag="OO464_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4124..4342)
                     /gene="hha"
                     /locus_tag="OO464_001105"
     CDS             complement(4124..4342)
                     /gene="hha"
                     /locus_tag="OO464_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6226333.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4368..4742)
                     /gene="tomB"
                     /locus_tag="OO464_001106"
     CDS             complement(4368..4742)
                     /gene="tomB"
                     /locus_tag="OO464_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6226334.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5288..8437)
                     /locus_tag="OO464_001107"
     CDS             complement(5288..8437)
                     /locus_tag="OO464_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6226335.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8460..9653)
                     /gene="acrA"
                     /locus_tag="OO464_001108"
     CDS             complement(8460..9653)
                     /gene="acrA"
                     /locus_tag="OO464_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6226336.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9795..10442
                     /gene="acrR"
                     /locus_tag="OO464_001109"
     CDS             9795..10442
                     /gene="acrR"
                     /locus_tag="OO464_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6226337.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10570..13932
                     /gene="mscK"
                     /locus_tag="OO464_001110"
     CDS             10570..13932
                     /gene="mscK"
                     /locus_tag="OO464_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6226338.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14144..14305)
                     /gene="rsmS"
                     /locus_tag="OO464_001111"
     CDS             complement(14144..14305)
                     /gene="rsmS"
                     /locus_tag="OO464_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6226339.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14319..14846)
                     /gene="priC"
                     /locus_tag="OO464_001112"
     CDS             complement(14319..14846)
                     /gene="priC"
                     /locus_tag="OO464_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6226340.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14916..15293
                     /locus_tag="OO464_001113"
     CDS             14916..15293
                     /locus_tag="OO464_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6226341.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15446..15997
                     /gene="apt"
                     /locus_tag="OO464_001114"
     CDS             15446..15997
                     /gene="apt"
                     /locus_tag="OO464_001114"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6226342.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16126..18051
                     /gene="dnaX"
                     /locus_tag="OO464_001115"
     CDS             16126..18051
                     /gene="dnaX"
                     /locus_tag="OO464_001115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6226343.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17384..17448
                     /gene="dnaX"
                     /locus_tag="OO464_001115"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18104..18433
                     /locus_tag="OO464_001116"
     CDS             18104..18433
                     /locus_tag="OO464_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6226344.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18433..19038
                     /gene="recR"
                     /locus_tag="OO464_001117"
     CDS             18433..19038
                     /gene="recR"
                     /locus_tag="OO464_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6226345.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19148..21022
                     /gene="htpG"
                     /locus_tag="OO464_001118"
     CDS             19148..21022
                     /gene="htpG"
                     /locus_tag="OO464_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6226346.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21203..21847
                     /gene="adk"
                     /locus_tag="OO464_001119"
     CDS             21203..21847
                     /gene="adk"
                     /locus_tag="OO464_001119"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6226347.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21979..22941
                     /gene="hemH"
                     /locus_tag="OO464_001120"
     CDS             21979..22941
                     /gene="hemH"
                     /locus_tag="OO464_001120"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6226348.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22938..23897)
                     /gene="aes"
                     /locus_tag="OO464_001121"
                     /pseudo
     CDS             complement(22938..23897)
                     /gene="aes"
                     /locus_tag="OO464_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24049..25353
                     /gene="gsk"
                     /locus_tag="OO464_001122"
     CDS             24049..25353
                     /gene="gsk"
                     /locus_tag="OO464_001122"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6226349.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25486..27162)
                     /locus_tag="OO464_001123"
     CDS             complement(25486..27162)
                     /locus_tag="OO464_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR6226350.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27400..28620)
                     /gene="fsr"
                     /locus_tag="OO464_001124"
     CDS             complement(27400..28620)
                     /gene="fsr"
                     /locus_tag="OO464_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6226351.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28838..30490
                     /gene="ushA"
                     /locus_tag="OO464_001125"
     CDS             28838..30490
                     /gene="ushA"
                     /locus_tag="OO464_001125"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6226352.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30527..31006)
                     /gene="ybaK"
                     /locus_tag="OO464_001126"
     CDS             complement(30527..31006)
                     /gene="ybaK"
                     /locus_tag="OO464_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6226353.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31210..32004)
                     /locus_tag="OO464_001127"
     CDS             complement(31210..32004)
                     /locus_tag="OO464_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6226354.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32142..32483
                     /locus_tag="OO464_001128"
     CDS             32142..32483
                     /locus_tag="OO464_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6226355.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32698..35202)
                     /gene="copA"
                     /locus_tag="OO464_001129"
     CDS             complement(32698..35202)
                     /gene="copA"
                     /locus_tag="OO464_001129"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6226356.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35464..36396
                     /gene="glsA"
                     /locus_tag="OO464_001130"
     CDS             35464..36396
                     /gene="glsA"
                     /locus_tag="OO464_001130"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6226357.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36399..37691
                     /locus_tag="OO464_001131"
     CDS             36399..37691
                     /locus_tag="OO464_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6226358.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37816..38223
                     /gene="cueR"
                     /locus_tag="OO464_001132"
     CDS             37816..38223
                     /gene="cueR"
                     /locus_tag="OO464_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6226359.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38224..38682)
                     /locus_tag="OO464_001133"
     CDS             complement(38224..38682)
                     /locus_tag="OO464_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6226360.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38679..39596)
                     /locus_tag="OO464_001134"
     CDS             complement(38679..39596)
                     /locus_tag="OO464_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6226361.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39742..40419
                     /gene="fetA"
                     /locus_tag="OO464_001135"
     CDS             39742..40419
                     /gene="fetA"
                     /locus_tag="OO464_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6226362.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40406..41185
                     /gene="fetB"
                     /locus_tag="OO464_001136"
     CDS             40406..41185
                     /gene="fetB"
                     /locus_tag="OO464_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6226363.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41248..42102)
                     /gene="cnoX"
                     /locus_tag="OO464_001137"
     CDS             complement(41248..42102)
                     /gene="cnoX"
                     /locus_tag="OO464_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6226364.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42163..42972)
                     /gene="ybbO"
                     /locus_tag="OO464_001138"
     CDS             complement(42163..42972)
                     /gene="ybbO"
                     /locus_tag="OO464_001138"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6226365.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42962..43584)
                     /gene="tesA"
                     /locus_tag="OO464_001139"
                     /pseudo
     CDS             complement(42962..43584)
                     /gene="tesA"
                     /locus_tag="OO464_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43555..44241
                     /locus_tag="OO464_001140"
     CDS             43555..44241
                     /locus_tag="OO464_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6226366.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44238..46653
                     /locus_tag="OO464_001141"
                     /pseudo
     CDS             44238..46653
                     /locus_tag="OO464_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47366..48460)
                     /gene="mnmH"
                     /locus_tag="OO464_001142"
     CDS             complement(47366..48460)
                     /gene="mnmH"
                     /locus_tag="OO464_001142"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6226367.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48529..49455)
                     /gene="allS"
                     /locus_tag="OO464_001143"
     CDS             complement(48529..49455)
                     /gene="allS"
                     /locus_tag="OO464_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR6226368.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49685..50167
                     /gene="allA"
                     /locus_tag="OO464_001144"
     CDS             49685..50167
                     /gene="allA"
                     /locus_tag="OO464_001144"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR6226369.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50245..51060
                     /gene="allR"
                     /locus_tag="OO464_001145"
     CDS             50245..51060
                     /gene="allR"
                     /locus_tag="OO464_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR6226370.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51150..52931
                     /gene="gcl"
                     /locus_tag="OO464_001146"
     CDS             51150..52931
                     /gene="gcl"
                     /locus_tag="OO464_001146"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR6226371.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52944..>53217
                     /locus_tag="OO464_001147"
     CDS             52944..>53217
                     /locus_tag="OO464_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR6226372.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12830 a        13693 c        14017 g        12677 t
ORIGIN      
        1 tccgcccgga gagggttagt agttccagcg ataacgctgc aagtcgattt gcccgcttcc
       61 cgataccatc acaccttctg ccagtaatgc ctgtcgctga cgctgtaaat ccggcccggt
      121 tagcgaaatt gtgccatggc gattaaccac ccggtgccag ggtaaggtgc tgccttcagg
      181 gagacgcttt aacacaccgc ccacctggcg cgcggcgcgg ggcgatcccg ccagtttcgc
      241 cacatcaccg taagtggtga catagccttc gggaatagcg gcgacgattt gccagacgcg
      301 ttggggaaat gaatcttctt tttccatctt ttcttcctga ggtaattttt cagcataatc
      361 tggaaaaacg cccgagtgaa gtcgcattgc gcaagaaacc agcatctggc acgcaatggg
      421 ttgcaattag ccggggcagc agtgataatg cgcctgcgcg ttggttttca acgctctcaa
      481 tgggggctct gttggttctc ccgcaatgct actctgttta ccaggtcagg tccggaagga
      541 agcagccaag gcagatgacg cgtgtgccgg gatgtagctg gcagggcccc cacccatttc
      601 tgcctcccac cgtttcgtca aaaaatccca gcatggctaa actttaacca caactgacgt
      661 cgcaagaatt gtctggctgc gcagtacgct tcggaggtat gtctgatgaa gtatgttgat
      721 ggttttgtgg ttgccgttcc tgccgataaa aaggatgcct atcgggaaat ggccgctaag
      781 gccgcgccat tgtttaaaga gtttggcgcg cttcgtattg tcgaatgctg ggccagcgat
      841 gtgccggatg gcaaagtgac cgattttcgt atggcggtga aagcggaaga gaatgaagag
      901 gttgtcttta gctggattga atacccttca aaagaggtcc gcgacgcagc taatcaaaag
      961 atgatgtcgg acccacggat gaaagagttc ggcgagtcca tgccgtttga cggcaagcga
     1021 atgatctatg gcggattcga gtcaatcatc gacgaatagg tagcgtgacg ggctgtgcgg
     1081 cgtgccgtca gcccaaaaat tcacaaatgt tgctcggccc agcgtaagaa atcttctttc
     1141 ggtaatgcct tgctgtagag ccagccctga ccgtagtgca cgccatgctg gcgtagccac
     1201 tcttcttgtt tactggtttc gattccctcc gctaccattt tcagtttcag tgtttttgcc
     1261 atttcgatga tatgcggcgt gacattttta tattccagcg catcaacgaa agatttatcg
     1321 atcttcagaa tgtcgacatc cagatcctgt aaataactta aacttgaata ccccgtacca
     1381 aaatcatcaa gataaatttc atggcccgcc tcccggtagc gagaaattat cggggcgctg
     1441 gttttcggat cggcaaactc gcgttcagtg agttcaagcg cgatctgtct gggattaacc
     1501 tgatagtgat tgatcatttc acgcagcaat tgcgggattt tttccgaggt gagcacggtg
     1561 gattcaagat tgatcgaaat atgctgctgt ggatgctgac gcaagcagtc gcccatatct
     1621 tcaaagacgc ttcttataat cagtagcgtc aatggctcag aaaggcccgt ttgctgtgcc
     1681 agcggaataa aactatctgg tgacaaccaa ctaccgtctg tctgcggcca gcgcgccagt
     1741 gcctcagcac cgacaatttt gccattggct aaggagacaa tcggctgata gtgcacgcaa
     1801 atatcacgat tttcgatagc atcctgcagc cgatgatgcg gtgattggat acggcgcaaa
     1861 atacgcagca caaacatcgc tgccagcagg ccaatcacca acccgagcgg taaccaaata
     1921 aagacttgcc gatgccagcc tttctgcaac attttcgttg aagcccaggt gatgatcgaa
     1981 atattcatct ccaggaaggg cagtatatcg taaatgatcc cattattttc gatatgctca
     2041 ccgggtgttt tttgtagcct ggtaataatt ccctgagcaa tttcatcgct gctggttatg
     2101 acaacgttat gggcattgcc aataatggcg gcatcaattt gccatgaact atagggaatg
     2161 acatcaataa aggaagcggg gtcgatcatg acgacataat gtgccgttcc catggcgacc
     2221 atgtaacgga taatgcctaa atcgttatgc gatgttaacc agacacgata accatctttc
     2281 gaaattttac ctagctcggg aaaggtatcg ggcggacttt catgctccag agacgaacac
     2341 tggggaacgt tgttatcgat ataaaccact tcctgaatat agcgataact gtaagatacc
     2401 cgacgcattt ccatgagatg ggcttcgcta caggcagcgc cttgccatct ttccagctcc
     2461 tgcagcgcat ctttcccttg tgtcgccacc ttattggctc gaatagcgac gcgggaggaa
     2521 taggtatcca gctcttcaat aaacgatgtt tctacctgct gatgggccag ccagatgctt
     2581 aagccgacag gcagcaatac tgaaagaatc agtactcccg aaataaggcc gaccagatgt
     2641 cgtgttctca ccagcatatc cttattaaca cagtgtaggg aaagtatatc tgattgtgga
     2701 aaaaggctgg caggagataa ggaggtagag ctggtatttc atgaagtgat gggtggaaac
     2761 gatcattgtc atttgaccag gcgcagagct ttacgcctgg tatgtaaccc taacttaaga
     2821 tgttgattcg gcgcgagcga gggtaaaaat atcgtaaaaa gacagttcac ctttacggac
     2881 gatcattttt tgcagttgtt ccttatgaac atccgcgaca cgcggagagt tcaagatggc
     2941 ttcaaccagc gcggcaggta caaagcgcgt gttgtaccat tcgccgttat agcaaacgcg
     3001 gaaatccagc acgtcgatat cttcatagcg gtagcgtggg tagacatcga aaaagaagaa
     3061 accattaagc aggataaata atacaaccag tagccagaca gagcctgaca gcgtttcgga
     3121 ctgcaacatc accgccaggg tggcaaaaaa agcaacgtac ataccgataa acagccccgg
     3181 atgtttacgg ataaaactga tactaaagcg gggtttgttg tcgcgttttt cgcgggtatt
     3241 cagagactca atcgtttcgg tcagcaggcg ttgtatttcg gtcatcgttt gccttcgtgg
     3301 tatctgcaac tttccaggga acaccctatt ttaacgtggg aatagtagag gggaagtaac
     3361 agatctacca caattttgca taacagttgc gaaaaaccga ttacaatttt ttaattattc
     3421 tggctggatt accgcccacg acaacgttgt ccgggacacc ttttgtgaca actgcacctg
     3481 aagctaccac gacgttatca ccaatggtca caccagggtt aatgaccgcg cgtccgccaa
     3541 tccagacgtt attaccgatg gtgacgggtt tccccagttc agcaccgcta ttacgtgcta
     3601 cagggtcgat gggatgtgtt gccgtgtaga tatgaacgcc tggtgccaac atacagttat
     3661 caccgatgcg aatagggcag acatcaagca tcacgcaatc gaagttggcg aaaaaattat
     3721 tgccgagaaa aatgttatag ccataatcac agcgaaacgt tggctcaata taagcctctg
     3781 tcacctgacc gaataaatta gcgagaattt actggcgtaa tgtgtgctct tccgccaggg
     3841 aatgattgta tcggtgaata agctgacgag cgcgcaggcg atcgcgagat aacgtctcat
     3901 ctgccgagcg atacaactca ccagcaatca tcttttcttt ttctgtgctc attactgacc
     3961 tccggttgct gaaaatgcgg caacacagta agacgaaaag tcagtgaaga gaacgttctc
     4021 attacatctg tgtgataaag atcacatagg gtatgtcttc atggcgaaaa agtataaaat
     4081 tcttaataaa cagccggtta tagcaccgaa agcgaattat ttattagcga ataaatttcc
     4141 atactgagga agggatcttg tcgtacagtt tattcatggt caattcggcg aggcggtgat
     4201 ctgcggctga gtaaaatacc gccagttcat tatctgataa ttcgtattta tttttctcga
     4261 taacacgctc cagcgtgtca attgtctggc aacgacgtaa acgcattaaa taatcggttt
     4321 ttgttaaagg tttttcggac atacttctac ccatggttgt aataattcta acaagataaa
     4381 ctcgcaggat tctctttcgt cgcgttgaca aaacaacgga atagtcgatt acctgacttc
     4441 cgccatttct gaagagcctg catattaata ccataactgc tgaacaacat aaaggtgtca
     4501 tccagatatt cgtcgatctg ctcaatgagc ttattgtctt cattatactt aattttgtaa
     4561 ttaagtgcga aggtcgcaat atgctcaatc agttcattca actggaggtt gatcgccgag
     4621 gttgggtcgt taacccaacc atgattgctt tcttcaaggt ttgcaagaca gtcatgatac
     4681 agggtttcac agagaaactt aagctgtgcg atatcatgtc ttttgggtga gtattcatcc
     4741 ataacgcgtc cccttcttag tggttgaact aacggacacc tttcgggatg gaaaaaactt
     4801 actgacctag acttgccctc tcgtttgtta gcttaactat aagccactct ttgcaggttt
     4861 tcatcgcatt attacgaaac attacaatta ggaaggaatc gggacaggac tcactttttc
     4921 atactagcac taacccgcag caggtaaaag cagtttgctc gaactgttca taacatttca
     4981 ggattatcgc cagatgaaat tggtgacact aataccagga ttgctctgaa tatgacgtaa
     5041 taaccgagga atgaataaag aattaccgca aataattaag aatagcctct aaatgattat
     5101 ggattattaa atctattagc gctacttaat ataaattaac taacaatcag tattttttat
     5161 gaattttata cgtggttaat actggttttc gtatgagatc ctgagttggt ggttcaatta
     5221 ctccttaatg ttcgtaggtt atgcataaaa aaggccgctt acgcggcctt agtgattaca
     5281 tgttgtatca atgatgatcg acagtatggc tgtgctcgat atcttcattc ttgcggctaa
     5341 agcggcggcg aaccaccaca aagaataccg gaacgaagaa gattgccagt accgttgcgg
     5401 tcaccatccc gcccattaca ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag
     5461 tactgataac cagcggcata acgccgagga taaacgccag cgaggtcatc aggatcggac
     5521 gtaaacgcat ccgcaccgca tcaagcgtcg cttcaatcag acctttacct tctttatcca
     5581 tcaagtcttt ggcgaattcg acgataagta tcgcgttctt cgccgacaac ccaatggttg
     5641 tgagcagtcc tacttggaag taaacgtcat tggtcaggcc acggaaggtg gcagccagca
     5701 acgcaccgat aacccccagc ggaacgacca gcataacgga gaacggagtc gaccagctct
     5761 cgtacagcgc cgccagacac aggaatacga caatcaacga aatcgcgtac agtgaaggtg
     5821 cctggttgcc ggagagacgt tcctgatagg acatccccgt ccagtcatag ccaacaccgg
     5881 taggcagttt gctcgccagt tgttccatca gctccattgc ttcaccggta cttttacccg
     5941 gtgccgcctg gcctaagatt tccatggatg gcaggccgtt gtaacgttcc agacgcggcg
     6001 aaccgtactc ccaacgagaa gaggagaacg ccgagaatgg caccatctga ccatcagcag
     6061 cacgaacata ccagtcgccg atatcatccg gcagcatacg gtatttcgct tctgacatga
     6121 cgtaaacttt cttcacacga ccgcggtcga taaagtcgtt cacatagcta ccgccccatg
     6181 cagcgcccag agtggtgtta atgtcgttga tagaaacacc cagcgcctgc gctttttcct
     6241 ggtcgatatc aatcttaaac tgcggggtat cttccagacc gtttggacgt acgctggtca
     6301 gcatatcagg gtgcttcgct gcttctgcaa gcagctggtt acgcgcctga gtcagttttt
     6361 cgtgaccaag gccagcctgg tcaatcagct caaagtcaaa gccggttgca gtacccagtt
     6421 ccacgattgc gggcaggtta aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc
     6481 gtgttgcacg catggtaatc gcttcaactt tgttttcttc gcccggacga tcggcccagt
     6541 ccttcaagga aacgaacgca ataccggtat tctgaccacg tcccgcaaag ccgaagccgt
     6601 taacggcgaa caccgactca acgttgttct tttctttggt cagatagtaa tgcgttacct
     6661 cattgagcac tttctgcgta cgttcctgcg ttgcacctgc tggcagctga accatggtca
     6721 taaatacgcc ctggtcctca tctggcaaga aggagcttgg cagacgcacg aacagatagg
     6781 ccatgccgac cacgatgatc agatacagca ctaggtaacg ccccgtactg cgcagaatac
     6841 cgcctacgct gtcggtgtag tggtgcgtgc tcttctcgaa catgcggttc aaccagccga
     6901 agaagccttt tttaccttcc ccgtgatcgc ctttggcaat cggtttcagc atggtggcac
     6961 aaagagccgg agtcaggatc aacgctacca gtaccgacag cgccattgct gaaacaatgg
     7021 taatagagaa ctgacgatag atagcaccag tagaaccgcc aaagaaggcc atcggtacga
     7081 ataccgccga cagtaccatc gcgataccga ccagagcgcc ctgaatctgc cccatcgact
     7141 tacgggtagc ttcttttggc ggcaaacctt cttccgccat aacacgctcc acgttttcta
     7201 ccacaacgat agcgtcatcc accaacaagc cgatggcgag caccatcccg aacattgtta
     7261 gcgtgtttat cgagaagcca aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg
     7321 gtacggcaat ggtcggaatc aacgtcgcgc ggaagttctg caggaacaga tacataacca
     7381 ggaacacgag gatgatcgct tcgaccagcg ttttaaccac ttcgtgaata gagattttca
     7441 cgaacggcgt ggtgtcgtac gggtaaacaa ttttcagacc cgacgggaag aacggttcca
     7501 tcttcgccag ttcagcacgg attgccgcag cggtatccag cgcgtttgca ccggtcgcca
     7561 gcttgatccc cagaccggaa gcaggttggc cgttaaactc tgcgatgatg tcgtagttct
     7621 caccacccag ctcaatcttc gccacatcac gcagcagtac gcgggaacca tcctgattca
     7681 ctttcagcag gattttgccg aactcttcag tagaggtcag acgcgtctga gcaataatag
     7741 aggcgttaag ctgttggcct ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct
     7801 gggcgttctg cgctttgatg gcggtaatga catcaaccgg cgttagctgg aatttgttca
     7861 gctcattcgg gttcatccag atacgcatcg cgtactgtga accgaacaac tgaacatcac
     7921 ccacgcccga cgtacggctg atggcatctt tcatattcgc cgctacgtag tcagagatat
     7981 cctcctgcgt catggtgcca tcggtgttga taacgccgac aaccatcagg aagctgctgg
     8041 atgatttctc aacgctcacc ccttgctgct gaacttcttg cggcagcaac ggcatcgcca
     8101 gctgcagttt gttctgcacc tgaacctgcg cgatatccgc atcagtacca gactcaaagg
     8161 tcagggtgat ctgcacggta cccgtggagt cactgttaga ggacatgtac atcaggttat
     8221 cgataccgtt catattctgt tcgataacct gtgtcaccgt gtcctgcact gttttcgcat
     8281 cagcgccggg gtaggaggcg gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg
     8341 ccaccggcag tttgaggatc gccagccccc ctgccaacat gatgataatg gcgatcaccc
     8401 acgcaaaaat cgggcgatcg ataaagaaat taggcatgtc ttaacggctc ctgtttaagt
     8461 taagacttgg actgttcagg ctgagcaccg cttgcggctt gctggttatt atcagcggta
     8521 acttcttgtg cttttacctg gacaccagga cgcactttct gcagcccact tattactacg
     8581 cgatcgcctg ctttcagacc ttctgtcacc agccacttat cgccaatagc ctggcttgca
     8641 acgatcggac gggtttccac tttgtcatcc gcgccaacta ccagtacggt ggcatcacca
     8701 cgcggcgtac gggttacgcc ctgttgcggg actaaaatag cgtttggatt aagcccttct
     8761 tccagacgtg cgcgcacgaa catacccggc agcagagtgt gatccgggtt cgggaagata
     8821 gcgcgtaggg tgatagaccc agtggtctga tcaacggtaa cgtcagagaa ttccagcgta
     8881 ccgtcctgcg ggaacttaat gccgtcactg gtgatcagcg acactttggc tttgccgttc
     8941 tcttgtttca gcgtgccatt cgccagttcc tgtttcaggc gaaggaagtc gttgctggac
     9001 tgggtcacat caacgtagat cggatcaagt tgctgcacgg ttgccagcac agtcgcctga
     9061 ccgttctgta ccaatgcgcc ttccgtcacg ttcgacttac caatgcgacc gctaatcgga
     9121 gaggtgactt tggtgtaagc cagattgatc cgcgcagttt caacggcagc tttcgccgca
     9181 gttaccgcag cattcgcctg ttgcgcatca gccagagcct gatcgtaccc ttgcttactg
     9241 atgtactgag taccgagcag tttctgataa cgattcaccg tcaattgcgc gatattggct
     9301 gcagcctggg ctttcgccag atcacctttc gcactgtcgt atgtcgcctg ataggtcgca
     9361 ggatcaatct gatagagaga gacaccagct tcgatgtcgc taccttcttt gaaattacgc
     9421 ttcaggataa tcccgctaac ttgaggacga acttctgcga tccggtaggc actggtgcga
     9481 cctggaagct cggttgtgat ctgcagaggt tcagttttga ctgttactac gccaacggcg
     9541 ggcatctgct ggccaccttg ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg
     9601 cctgagagca tcagaacgac cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa
     9661 cctcgagtgt ccgatttcaa attggtcaat ggtcaaaagt taataaaccc attgctgcgt
     9721 ttatattatc gtcgtgctat ggtacataca ttcacaaatg tatgtaaatc taacgcctgt
     9781 aaattcacga acatatggca cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca
     9841 tcctcgatgt ggctctacgt cttttctcac agcagggggt atcatccacc tcgctgggcg
     9901 agattgcaaa agcagctggc gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt
     9961 cggatttgtt cagtgagatc tgggaactgt cagaatccaa tattggtgaa ctagagcttg
    10021 agtatcaggc aaaattccct ggcgatccac tctcagtatt aagagagata ttaattcatg
    10081 ttcttgaatc cacggtgaca gaagaacgac gtcgattatt gatggagatt atattccaca
    10141 aatgcgaatt tgtcggagaa atggctgttg tgcaacaggc acaacgtaat ctctgtctgg
    10201 aaagttatga ccgtatagaa caaacgttaa aacattgtat tgaagcgaaa atgttgcctg
    10261 cggatttaat gacgcgtcgg gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg
    10321 aaaactggct ctttgccccg caatcttttg atcttaaaaa agaagcccgc gattacgtcg
    10381 ccatcttact ggagatgtat ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat
    10441 aaccctgaat ctgactccag gatttttcct ggacattttc gtcgttgcta ttctggttca
    10501 ctgcgtcgtg atattcttgc ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct
    10561 tcattcatca tgactatgtt ccagtattac aaacgatcac ggcattttgt tttttcagca
    10621 tttattgctt ttgtttttgt cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg
    10681 aatggtgatc tgccgacaaa agcggacctg caggcgcaac ttgactcact aaataaacaa
    10741 aaagatcttt ctgctcagga caaactggtg cagcaggatc tgacagatac attagccacc
    10801 ctcgataaaa tcgatcgcat aaaagaagag acagttcagc tacggcaaaa agtcgctgaa
    10861 gcgccggaaa aaatgcgcca ggcgaccgcg gcgttaacag cacttagcga tgtcgataac
    10921 gacgaagaaa cgcgcaaaat tctgagtacg ctttcgttgc gccagctgga aactcgcgtt
    10981 gcccaggcgc tggacgattt gcaaaacgca caaaacgatc tggcgtctta taacagccag
    11041 ctggtttcgt tacagacgca gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag
    11101 cagctgcaac aaattcgcag tcgtctggat gggactgatg tcggcgagac tgccttacgt
    11161 cccagccaga aagtgttaat gcaggctcag caggcgttgc tgaatgcgga gattgaccag
    11221 cagcgtaaaa gcctggaagg gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat
    11281 tacgtgacgg cgaacagcgc ccgtctggag caccagttgc agctgttgca agaagcggta
    11341 aacagcaagc gcctgacttt aactgaaaaa acggcgcagg aagccgtctc cccggatgaa
    11401 gccgcgcgta ttcaggctaa tccgctggtg aaacaggaac tggaaattaa ccagcagtta
    11461 agtcagcgtc tgattaccgc gactgaaaac ggtaatcagt tgatgcagca aaacattaaa
    11521 gtcaaaaact ggctggagcg ggcgctgcaa tcggaacgca atattaaaga gcagattgcc
    11581 gtcctgaagg gcagcctgct gttgtctcgt atcctttacc agcaacaaca aacgctgccc
    11641 tcggcggatg aactggaaaa catgaccaac cgcatcgcgg atttgcgtct cgaacagttt
    11701 gaagttaacc agcagcgtga tgcactcttc cagagcgatg cgttcgtcaa caaactggaa
    11761 gaaggtcaca ccaacgaagt caacagcgaa gttcacgatg cgttattgca agtggttgat
    11821 atgcgtcgcg aattgctgga tcaactcaac aaacagttgg gtaaccagct gatgatggcc
    11881 attaacctgc aaatcaacca gcagcagtta atgagcgtat cgaaaaacct gaaatccatc
    11941 ctgactcagc aaatcttttg ggtgaacagt aaccgtccaa tggactggga ctggatcaaa
    12001 gcgttcccgc aaagcctgaa agatgaattt aagtcgatga aaatcacggt gaactgggaa
    12061 aaagcctggc ctgccgtttt tatcgctttc ctcgccggtt tgccgctgct gttgattgcc
    12121 gggctgatcc actggcgtct gggctggctg aaagcgtatc aacagaaact ggcttccgct
    12181 gtgggttccc tgcgtaacga tagccagctc aacacaccaa aagcgatcct tatcgacctg
    12241 atccgcgcgc tgccggtgtg cctgattatt ctcgcggttg gcctgattct gttgaccatg
    12301 cagctcaaca tcagcgaact gctatggtcg ttcagtaaaa aactggcgat tttctggctg
    12361 gtgtttggcc tgtgctggaa ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc
    12421 atgccggagc agcagaccag ccactggcgt cggcaaattg tccgcatcag tctcgcgttg
    12481 ctgcctatcc atttctggtc tgtggtggca gaactttccc cgctgcatct gatggatgat
    12541 gtgctggggc aggcgatgat tttcttcaac ctgctgctga ttgccttcct ggtatggcca
    12601 atgtgccgcg aaagctggcg tgataaagag tcgcacacca tgcgactagt caccattacc
    12661 gtgctgtcga ttatcccgat tgcattgatg gtgctgactg ccacgggtta tttctacacc
    12721 acgctgcgcc tctccgggcg ttggattgaa accgtttatc tggtgatcat ctggaacttg
    12781 ctctaccaga cggtattgcg cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt
    12841 gcgctggcgc gtcggcagaa tctggtgaaa gagggcgcag aaggtgccga accgccggaa
    12901 gaacccacca ttgcactgga gcaagttaac cagcagacgc tgcgtattac catgttgctg
    12961 atgtttgcgc tgttcggtgt catgttctgg gcaatttggt ccgatttgat caccgtgttc
    13021 agctatctcg acagcatcac gctctggcat tacaacggca ctgaagctgg cgctgcggtg
    13081 gtgaaaaacg tcaccatggg cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg
    13141 gcgttgattc gcaacctgcc tggtttactg gaagtgctgg tgctctcgcg actgaatatg
    13201 cgccagggcg cgtcgtatgc cattactacc atccttaact acatcattat tgccgttggt
    13261 gcgatgacgg tgttcggatc gctgggcgtc tcttgggata aactccagtg gctggccgca
    13321 gcattatccg taggtcttgg ttttggttta caagaaattt tcggtaactt cgtctccggt
    13381 ttgatcattc tgttcgaacg tccggtgcgt attggcgata cggtaaccat tggtagcttc
    13441 tcggggacgg taagtaagat ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa
    13501 gaagtgatca tcccgaacaa agcgtttgtt accgagcgtc tgatcaactg gtcgttgact
    13561 gacactacta cgcgtctggt gatccgtctc ggcgtggcct atggctccga tctggaaaaa
    13621 gtgcgtaaag tgttactgaa ggcggcgact gagcacccaa gggtgatgca cgaaccaatg
    13681 ccggaagtct tctttacggc atttggtgcc agcacgttgg atcatgagct gcgtctgtat
    13741 gtgcgtgaac tgcgtgaccg tagccgtact gtcgatgagc tgaaccgtac tatcgatcag
    13801 ctgtgccgtg aaaacgacat caacattgcc tttaaccagc ttgaagtgca tctgcacaac
    13861 gagaagggcg atgaggtgac ggaagtaaaa cgcgactaca aaggcgacga tccgacacca
    13921 gcggttgggt aataaccaaa gggggcgaca tttagttgcc ccgagattac tgacaaagtg
    13981 cgcgttgttc atgccggatg cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat
    14041 tcaatatatt gcaggagcgg tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt
    14101 ttgcccgcaa tcttagggga catttagcgg ccccatttat ttctcacttt tccgcctcat
    14161 catcgtgcgt taatttcttt tcataatcac gctttacaat atccagcgcg cgcagcacgg
    14221 tactggcagg gatctgattt tcctccagca gcacaatcaa atcgacagcc agtttgacat
    14281 cgtcaggggc attttcaagt gacatactct ttccattgct agcgggttaa gcgcgctagc
    14341 ctgttttcga ttttttccag cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc
    14401 acttcacgat gcagcgtttg ctgttccacg agatcggtca cccgcgccag gcgggctctg
    14461 cgttcggcaa ccatctcacg tagccgccgc tcaaaatcct gatgctgaat acgtttacgc
    14521 tgccagcggg aaattttcgg tggcgcactg tcccactcgc gcaatgacca ggcggaggct
    14581 tcacgcgcga tggcttccag ttgtgccgcc agatgttccg ccagccaggc cacttgcggc
    14641 agctgttgct gctcaactgc atgacgaagc gcagccagat tatcgcccgc ctcgtcgaga
    14701 caagactgta gtgttgtcgc acgagtctga aaaagatgcc tgtcgaaacg agcacttagc
    14761 gtggcgaact gtgacaccgg ggcacaacgc tgacgcagcg tagcgagctg tccttccagt
    14821 ttttccagca gcagggcggt tttcacgata agacctcaat gaaaatgata attgttatgc
    14881 taaagtagcc actatgtaag ctgacaacac tcaatatgca acgaatcatt ttaatcatca
    14941 ttggctggct ggcggtagtg ctgggtacgc tgggcgtggt attaccggta ttaccgacga
    15001 cgccgtttat cctgctggcg gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct
    15061 ggttgctgta ccgctcatgg tttggcagct atctacgttt ctggcagaaa catcatgcga
    15121 tgccgcgcgg cgtcaaaccg cgggcgattt tgcttatttt gctcacgttt gccatttctc
    15181 tgtggttcgt ccagatgcca tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc
    15241 ttttctatat gtggcgaatt ccggtgattg atgaaaagca agaaaagcac tgaagcacaa
    15301 caatcgcagt tgcaattatt gcgtacagcc agtacattct ggcgttttcg agcacaggcg
    15361 caggcggtca aaggttaaac aacagttact tttgatacgt ttaaaacgcg ccgtgagtac
    15421 caccgtaaca agcaggcata cacttatgac cgcgactgca cagcagcttg agtatctcaa
    15481 aaatagcatc aaaagcattc aggactaccc aaaacccggc attcttttcc gcgatgtcac
    15541 cagcttactg gaagacccga aagcttacgc tctcagcatc gacttgctgg ttgagcgtta
    15601 caaaaatgcg ggcattacca aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc
    15661 tccggtagct ctgggtctgg gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc
    15721 gcgtgaaacc atcagtgaaa attacgacct ggaatacggc accgatcagc tggaaatcca
    15781 cgttgatgcc atcaaaccgg gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg
    15841 cggcactatc gaagcgaccg ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc
    15901 tgcgttcatt atcaacctgt ttgatctcgg cggcgaacag cgtctcgaaa aacagggcat
    15961 taccagctac agccttgtcc cgttcccggg ccattaatta tcgccagtct tgtgctgccc
    16021 acgctacgga cagcacaaga tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc
    16081 attacccctt cgtgaatcca ccttccagcg tttcagagcc tgccaatgag ttatcaggtt
    16141 ttagcccgaa aatggcgccc acaaaccttt gctgacgtcg tcggccagga acatgtgctg
    16201 accgcactgg cgaacggctt gtcgttaggg cgtattcatc atgcttatct tttttccggc
    16261 acccgtggcg tcggaaaaac ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa
    16321 accggcatta ccgcgacgcc gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg
    16381 cgctttgtcg atctgattga aatcgacgcc gcctcgcgca ccaaagttga agatacccgc
    16441 gacctgctgg ataacgtcca gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc
    16501 gacgaagtgc atatgctgtc gcgccacagc tttaacgcac tgttaaaaac ccttgaagag
    16561 ccgccggagc acgttaagtt tctgctggcg acgaccgatc cacagaaatt gccggtgacg
    16621 attttgtcac gctgtctgca atttcatctc aaggcgctgg atgtcgagca aattcgccat
    16681 cagcttgagc acatcctcaa tgaagaacat atcgctcacg agccgcgggc gctgcaattg
    16741 ttggcgcgcg ccgctgaagg cagcctgcga gatgccttaa gtctgaccga ccaggcgatt
    16801 gccagcggtg acggccaggt ttcaacccag gcggtcagtg cgatgctggg tacgcttgac
    16861 gacgatcagg cgctgtcgct ggttgaagcg atggtcgagg gcaacggcga gcgcgtaatg
    16921 gcactgatta atgaagccgc tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg
    16981 ctcggcttgt tgcatcgtat tgcgatggta caactttcgc ctgctgcact tggcaacgac
    17041 atggccgcca tcgagctgcg gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt
    17101 cagctttact atcagacgct gttgattggt cgcaaagaat taccgtatgc gccggaccgc
    17161 cgcatgggcg ttgagatgac gctgctgcgc gcgctggcgt tccatccgcg tatgccgctg
    17221 cctgagccag aagtgccacg ccagtccttt gcacccgtcg cgccaacggc agtaatgacg
    17281 ccaaccctgg tgccgccgca atcagcgccg cagcaggcac cgactgtacc gctcccggaa
    17341 accaccagcc aggtgctggc ggcgcgccag cagttgcagc gcgtgcaggg agcaaccaaa
    17401 gcaaaaaaga gtgaaccggc agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg
    17461 gaaagactgg cttcggtcac cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa
    17521 aaagcgccag ccaaaaaaga agcgtatcgc tggaaggcga ccactccggt gatgcagcaa
    17581 aaagaagtgg tcgccacgcc gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg
    17641 gaactggcgg tgaagctagc ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg
    17701 agccaacttt cgctaccaaa actggtcgaa caggtggcgt taaatgcctg gaaagaggag
    17761 agcgacaacg cagtatgtct gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt
    17821 gcacagcaaa aactggctga agcgttgagc atgttaaaag gttcaacggt tgaactgact
    17881 atcgttgaag atgataatcc cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac
    17941 gaagaaaaac ttgcgcaggc gcgcgagtcc attattgcgg ataataatat tcagaccctg
    18001 cgtcggtttt tcgatgcgga gctggatgaa gaaagtatcc gccccatttg atcgtaagca
    18061 cagcttacgt tcgtcatcct taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg
    18121 tctgggtaac ctgatgaagc aagcccagca gatgcaagaa aaaatgcaga aaatgcagga
    18181 agagatcgcg cagctggaag ttaccggcga atctggcgca ggtctggtaa aagtgaccat
    18241 caacggtgca cacaactgcc gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa
    18301 agagatgctg gaagacctgg tggctgcagc attcaacgac gcagcacgtc gtattgaaga
    18361 aacgcagaaa gaaaaaatgg cctctgtatc ctccggaatg cagctgccgc ctggctttaa
    18421 gatgccgttc tgatgcaaac cagcccgctg ttaacacagc ttatggaagc actgcgctgt
    18481 ctgccgggcg ttggcccgaa gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat
    18541 cgtagcggcg ggatgcgtct ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac
    18601 tgcgccgatt gccgcacttt caccgaacag gaagtctgta acatctgttc gaatccgcgt
    18661 cgtcaggaaa acggtcaaat ctgtgtggtg gagagtccgg cggacatcta cgccattgag
    18721 cagacggggc agttttctgg tcgttatttt gtgttgatgg ggcatctgtc accgctggac
    18781 ggcatcggtc cggatgatat cgggcttgat cgtctggaac agcgtctggc agaggaaaaa
    18841 atcactgaag tgatcctcgc caccaatccc acggttgaag gtgaagctac cgctaactac
    18901 attgccgagc tttgcgcgca atatgacgtg gaagccagcc gaatcgctca tggcgtaccg
    18961 gttggcggcg aactggaaat ggtcgatggc accacgctgt cacactccct cgccgggcgt
    19021 cataagattc gtttttaagc aaacgagagc aggagcacct gctctcgctt gaaattattc
    19081 tcccttgtcc ccatctctcc ctcatcctgt ttttaacctt aaaatggcat tattgaggta
    19141 gacctacatg aaaggacaag aaactcgtgg ttttcagtca gaagtgaaac agcttctgca
    19201 cctgatgatc cattctctct attccaataa agaaatcttc ctgcgtgagc ttatctctaa
    19261 cgcctccgat gcggcggaca agctgcgttt ccgtgcgcta tctaacccgg acctgtacga
    19321 aggtgatggc gaactgcgcg ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat
    19381 ctccgataac ggcgtgggga tgacccgcga cgaagtgatt gaccatctgg ggactatcgc
    19441 taaatccggt accaaatcat tcctcgaatc cctgggttct gaccaagcga aagacagcca
    19501 gctgatcggt cagtttggtg ttggtttcta ctctgcgttt atcgtggccg acaaagtgac
    19561 cgtgcgtact cgcgcggcag gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg
    19621 cgaaggtgaa tataccgttg ccgatatcac caaagaagat cgtggtactg aaatcaccct
    19681 gcatctgcgt gaaggcgaag acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag
    19741 caaatactcc gaccatatcg cgctgccggt agagatcgaa aaacgcgaag agaaagatgg
    19801 cgaaaccgtt atctcctggg caaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa
    19861 gtcggaaatc accgatgaag agtataaaga gttctacaaa cacatcgccc acgactttaa
    19921 cgatccgctg acctggagcc acaaccgcgt tgaaggtaag caggagtaca ccagcctgct
    19981 gtacatcccg tcccaggctc cgtgggatat gtggaaccgt gatcataaac acggcctgaa
    20041 actgtacgtt caacgcgtgt ttatcatgga cgacgctgaa cagttcatgc cgaactatct
    20101 gcgcttcgtg cgtggtctga ttgactccag cgatctgccg ctgaacgttt cccgtgaaat
    20161 cctccaggac agcacggtaa cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca
    20221 aatgctggaa aaactggcga aagacgacgc ggaaaaatac cagaccttct ggcaacagtt
    20281 tggcctggta ctgaaagaag gtccggcgga agatttcgct aaccaggaag cgatcgccaa
    20341 actgctgcgt tttgcttcta cttataccga ttcttctgcg cagaccgtgt ctctggaaga
    20401 ctacgtttcc cgcatgaaag aagggcagga gaaaatctac tacatcactg cagacagcta
    20461 tgcggcagcg aagagcagcc cgcacctgga actgctgcgt aagaaaggca tcgaagttct
    20521 gctgctttcc gaccgcatcg atgagtggat gatgaactat ctgactgagt tcgacggtaa
    20581 accgttccag tcggtgtcta aagttgacga gtcgctggaa aaactggctg acgaagttga
    20641 tgagagcgcg aaagaagcgg agaaagcact gactccgttc atcgaccgcg tgaaagctct
    20701 gctcggcgag cgcgtgaaag atgtccgtct gactcaccgt ctgaccgata cgccagcgat
    20761 cgtttcgacc gacgcggacg aaatgagcac tcagatggcg aaactgttcg ctgcggcggg
    20821 ccagaaagtg ccagaagtga aatacatctt cgaactgaac ccggatcacg tactggtgaa
    20881 acgtgcggca gatactgaag atgaagccaa gttcagcgag tgggtagaac tgctgctgga
    20941 tcaggcgctg ctggcagaac gcggcacgct ggaagatccg aacctgttta ttcgtcgtat
    21001 gaatcagctg ctggtttcct gatgtcatgc cggatgacct ttgtgtcatc cggcattttt
    21061 cttttcatca tctgtacttt ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg
    21121 cctttcttga ggcaatcgcc tgttggtggt atcgtttatc gctttttcaa aaaaatcgac
    21181 acattttaag gggattttcg caatgcgtat cattctgctt ggcgctccgg gcgcggggaa
    21241 agggactcag gctcagttca tcatggagaa atatggtatt ccgcaaatct ccactggcga
    21301 tatgctgcgt gctgcggtca aatctggctc cgagctgggt aaacaagcaa aagacattat
    21361 ggatgctggc aaactggtca ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc
    21421 tcaggaagac tgccgtaatg gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc
    21481 agacgcgatg aaagaagcgg gcatcaatgt tgattacgtt ctggaattcg acgtaccgga
    21541 cgaactgatt gttgatcgta tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta
    21601 tcacgttaaa ttcaatccgc cgaaagtaga aggcaaagac gacgttaccg gtgaagaact
    21661 gactacccgt aaagacgatc aggaagaaac cgtgcgtaaa cgtctggttg aataccatca
    21721 gatgactgca ccgctgatcg gctactactc caaagaagcg gaagcgggta acaccaaata
    21781 cgcgaaagtt gacggtacca agcaggttgc tgaagttcgc gctgatctgg aaaaaatcct
    21841 cggctaatca agccgcagca gatctgcttt cgggcaggtc tgcttatcca cctcaggcaa
    21901 tacctctcac agcaattagt tcttcttcct cacttttccg ctacaattat caacaagttg
    21961 aatcgataag aggcggtaat gcgtcagact aaaaccggta tcctgctggc aaacctgggt
    22021 acgcccgatg cccccacacc tgaagcggta aaacgctatc tgaaacaatt tttaagcgac
    22081 agacgcgtgg ttgatacctc acggttgtta tggtggccgt tgctgcgcgg cgtgattttg
    22141 ccgctgcgct cgccgcgtgt ggcgaagctg tacgcctctg tctggatgga agacggctcg
    22201 ccgctgatgg tttacagccg ccagcaacag caggcgttgg cacaacgttt accggatacg
    22261 ccagtggcgc tgggcatgag ctacggctcg ccatcactgg aaagctccgt cgatgaactc
    22321 ctggcagagc atgtagatca tattgtggtg ctgccgcttt atccgcaatt ctcctgttct
    22381 acggtcggtg cggtatggga tgaactggca cgcattctgg cgcgcaaacg tagcattccg
    22441 gggatatcgt ttattcgtga ttacgccgat aaccacgatt acattaatgc actggcgaac
    22501 agcgtacgcg cttcttttgc caaacatggc gaaccggatc tgctgctgct ctcttatcat
    22561 ggcattcccc agcgttatgc agatgaaggc gatgattacc cgcaacgttg ccgcacaacg
    22621 actcgcgaac tggcttccgc actggggatg gtaccggaaa aagtgatgat gacctttcag
    22681 tcgcgctttg gccgggaacc ctggctgatg ccttataccg acgaaacgct gaaaatgctc
    22741 ggagaaaaag gcgtaggtca tatacaggtg atgtgcccgg gctttgctgc ggattgtctg
    22801 gagacgctgg aagagattgc cgagcaaaac cgtgaggtct tcctcggtgc gggcgggaaa
    22861 aaatatgaat atattccagc gcttaatgcc acgccggaac atatcgaaat gatggctaat
    22921 cttgttgccg cgtatcgcta aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc
    22981 tcgtcggcgg ttttcatcat ccgtgaataa tgcaaaaagg catgcagcgt acctgggtag
    23041 agtttgaact cacagggctg ctgatgcgcc gctaacgtct agtaaagcag acggctgtca
    23101 tccagcagcg gatcgaactc cgccccggca ataaaacagg gcggaacttc gcgagtgaga
    23161 tcattattaa acagacagta atacggtgac tcgcggtccg cgtcgttgct taaatacgcc
    23221 tcttcataca tctgcaaatc ctgttgcgtt aagccatccc agacaccgcc caacagacga
    23281 cgagtcacgg aatcccgtaa tccgtaaagc ccataccaca gcaaaacacc cgcaatttta
    23341 ccgcaatcga tctgtttatc acgcaaccac aacgcactgg cgagcgccag catggcacct
    23401 gcggaatcac cggcaaagcc aatgcgggac atattgattt gataatcctc cgcctgctgg
    23461 tggaaataac aacaagcagc cacaatttcc tctatcgctt gtggaaaacg cgcttcaggt
    23521 gaaagggtgt aattaatacc aatcaccgta cattggctgt agcttgccag caggcgcatg
    23581 atgcgatcgt gggtatcgag attgccgaga ataaaaccgc ctccatgcaa ataaaatagc
    23641 gtcgccgggc tatctggctg cggacaaaag agacgtgttt ccacctgccc atattttgtt
    23701 ggaaccatgt aagctctggt tgccatttct ggagcgcccg cattccagaa tcggcgctca
    23761 agcgtgtaat actgtcgttg ctcagcaatc gttcccgttg cgggccagga cggtaaatcc
    23821 ggctgaagag tattcacaac ggtcttcatt tcagcagaaa taaggtccag aacaggtagt
    23881 ttgttttccg gcttcataca aaaactcctt tcaaattacg tcattgtaag gaaccactgc
    23941 catgaaaatg cgatcccgcc tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc
    24001 agattgtgtt aacattcgcc gctcagttaa ccacccgtaa aaacaaccat gaaatttccc
    24061 ggtaaacgta aatccaaaca ttacttccct gtaaatgcac gcgatccgct gcttcagcaa
    24121 ttccagccag aaaacgaaac cagcgccgcc tgggtcgtag gtattgacca gacgctggtc
    24181 gatattgaag cgaaagtgga tgatgaattc attgagcgtt atggattaag cgccgggcat
    24241 tcactggtga ttgaggatga cgtagccgaa gtgctttatc aggagctaaa acagaaaaac
    24301 ctgattaccc atcagtttgc gggtggcacc attggtaaca ccatgcacaa ctactcggtg
    24361 ctcgcggacg accgttcggt gctgctgggc gtcatgtgca gcaatattga aattggcagt
    24421 tatgcctatc gttacctgtg taacacttcc agccgtaccg atcttaacta tctacaaggc
    24481 gtggatggtc cgattggtcg ttgctttacg ctgattggcg agtccgggga acgtaccttt
    24541 gctatcagtc caggccacat gaaccagctg cgggctgaaa gcattccgga agatgtgatt
    24601 gccggagcct cggcactggt tctcacctca tatctggtgc gttgcaagcc gggtgaaccc
    24661 atgccggaag caaccatgaa agccattgag tacgcgaaga aatataacgt accggtggtg
    24721 ctgacgctgg gcaccaagtt tgtcattgcc gagaatccgc agtggtggca gcaattcctc
    24781 aaagaccacg tttctatcct tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa
    24841 agcgatccgt tgttggcatc tgacaaggcg ctggactggg tagagctggt gctgtgcacc
    24901 gccgggccaa tcggcttgta tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc
    24961 cagcatccgc tgctgccggg cgctatagcg gaattcaacc agtatgagtt tagccgcgcc
    25021 atgcgccaca aggattgcca gaatccgctg cgtgtctatt cgcacattgc gccgtacatg
    25081 ggcgggccgg aaaaaatcat gaacactaat ggagcggggg atggcgcatt ggcagcgttg
    25141 ctgcatgaca ttaccgccaa cagctaccat cgtagcaacg taccaaactc cagcaaacat
    25201 aaattcacct ggttaactta ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg
    25261 agctatcagg tactgaacca gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa
    25321 gacagccagg aagagtctta ctgggatcgt taagttatcg tcggttcgta ggccagataa
    25381 ggcgttcacg ccgcatctgg catttggctc tcgatgcctg atgcgacgct ggcgcgtctt
    25441 atcatgccta catatttttc atattttaca tccggcaacc accgtttacc ccgtcaccac
    25501 ttcacccgcc ggtggcgttt ccagcagttc cagcatagta cgggcaattt cacgttcacc
    25561 catcactacc tgattcgcgc cacgttcggt gatatacgtc acttcatcgt cataatgggc
    25621 gcgggcgata atctcaatat ccgggttttt cgcgcgggca gacgccacaa tctcacccgc
    25681 ttcataaccg ttggggatcg tcaggatcag ccattttgca cattccagat gtgccagttg
    25741 cataatttct tcgttcgctg cattgcccaa tacagcgcgg accccacgct ctcgcagctc
    25801 atcaacacgg gttcgtgacg tctcaatcac caccagcgga atatcagagg cgagcaattt
    25861 ctcccccagc aggctgccta cacgaccgta acccaccaat agcgcatggt tgcaaatatc
    25921 cactgggatc tgtttctctt cttcgattgc ctcttccagc gtctgctctt ccagcgtttc
    25981 ggtcttcgcc agatatttct ccagtagtgc gaacagtacc gggttgagca taatcgacag
    26041 gatcgcccct gccagtacca ggttttgtcc ggcctgcggc agtaaattca atgccattcc
    26101 cagtcccgcc aggataaacg cgaactcacc aatctgcgcc aggctggcgg cgatggttaa
    26161 tgccgtacgt tgggagtgac caaacagacg gacaaggaac agggccgcga ccgatttacc
    26221 aaacagaata attgccagcg tcgccagcac tgccagcggt tgctgaatca gaattaacgg
    26281 atcaaacaac atcccgactg agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcaa
    26341 cgtatcgtgg gcggcacggt gactcaattc agactcgttc agtaccatcc cggcaaagaa
    26401 cgcaccgagt gcaaaggaga catcaaacag ctctaccgca ccaaaggcaa tccctaacgc
    26461 cagcgccagc actgacaggg taaacagctc gcgagaaccg gttgccgcgc tgcgtgccat
    26521 aatccacggc accagacggc gacctaccag catcataatg gcgataaatg cgatcacctt
    26581 gccgatggtg atccccatat cgactgcaag agtggcaaag cccacatcgc cctgttccat
    26641 cattcctgcc actgcgggca gcaacaccag cgtcagaacc atcaccaggt cttccacaat
    26701 caaccaaccg atggcgattt gcccacgctg actgtctatt aattgccgtt cttcaagtgc
    26761 gcgcagtaac accacggtac tggcggtgga aagacataag ccgaacacga taccggtcat
    26821 taacgaccag cccagcacgg cggagagcgc catacccagc agcgtcgcca cggctatctg
    26881 ggcgatcgcg ccgggaatgg cgatggactt taccgccatc aaatccttga gcgaaaaatg
    26941 caaaccgacg ccgaacatca gcaaaataac gcccagttca gccagttccg gcgcaagctt
    27001 ggtatcggca acaaagcccg gagtgaatgg tcctgccagc acacccgcta acagatatcc
    27061 caccagagga gaaatacgta gtttattggc cagcatgccg aggataaagg cgagcacaag
    27121 gccgccaaca atggtggtga taagcggggt ggcgtgatgc attccgtctc cttttcttgg
    27181 tggttattgt ccatttttgg ctgggaaaac caaaattaca ggtaatagtt tatgacaatt
    27241 tcattgatga tgtttatgaa taattgttga attttgcaga aaaatggaat tagctgcaaa
    27301 aaaagcacgg ttcggaaaac agaaggcggt ttaacagagg aaaggcttat ggcgactgta
    27361 aaacgatgca gccaaagttt gcctttggct gcaatgaaat cagtctttat gccggttatc
    27421 aggcaggaat atggtcaaca tccccaatag tggcaggaaa gcacagattt tatagactaa
    27481 ctcgatgctg gtgtgatcgg cgataagccc cagaactgcc gctcccagac ctcccatgcc
    27541 gaaagcaaaa ccgaaaaaga gtccagaaac cataccgata cgtcccggaa gtagctcctg
    27601 agcgtagacc agaatggcag agaatgccga agcgaggata aatccaataa tcaccgttaa
    27661 aacccccgtc cagtgcaggc gggcgtaggg taaaatcagc gtaaacggcg caacgccgag
    27721 gatagagccc caaatcacat atttccgtcc aattttatcc cctacaggcc cgccgatcac
    27781 cgtacctgcc gcaacggcaa acaggaaggc aaacagatga agctgggcat tctggataga
    27841 taatccgaat ttttgcatca gataaaaggt gtaatagctg ctgatgctcg ccatatagaa
    27901 atatttcgag aaaatgagga ttaacagaat gctgactgcc agtaccactt tgttgcgcgg
    27961 cagtggattg attatcgtcg ctttgggttt tcctttattc attcggtgct gtgccgagta
    28021 ccaacggcta atttgcgcca acaccacgat cgccagcagt gccgcaagca caaaccaggc
    28081 aacgttgcct ttgccataag gctcgataat caccgccgcc agcaagggtc ccagggaact
    28141 gccaaagttg ccgccgacct gaaagataga ttgcgccagg ccatgccgcc cgccggaagc
    28201 catacgggct acgcgagaag attccggatg aaagaccgat gaaccggtac cgaccagcgc
    28261 cgctgccagc agaactgcgc caaaactgcc cgccagcgca agcagcacca gaccacttaa
    28321 ggtaaagcac atgccaattg gcaacgacca cggcatcgga tatttatcgg tccagtagcc
    28381 gaccactggt tgcagtagcg aagaggcgag ctggaaggtg agggttatca tgccaatctg
    28441 cataaatgtc agagaaaatt ctgactgaag cagcggataa atcgccagaa tcagcgattg
    28501 gatcatgtcg ttcagcagat gtgagaggct gatagcacct aaaataccaa acgatgttcg
    28561 ggccttggtc gttgacgcag ccgcgcccgc cacaggctgg gtttgttcac tcattgccat
    28621 aggaaagtca ctttttcagg gttgcgatgt aaagaatgat cttatttgtg attattaccc
    28681 gactaacata cctgtatgcg tcgtctgaag gaagtctcaa cgccgaatac agaatttcta
    28741 atctggatgc agatttatct tcaccggacg cagacttgtc tatgatgttg cgtcatacta
    28801 tttttcaaca cgttgaaatc aggtcaggga gagaagtatg aaattattgc agcggggcgt
    28861 ggcgttagcg ctgttaacca catttacact ggcgagtgaa actgctctgg cgtatgagca
    28921 ggataaaacc tacaaaatta cagttctgca taccaatgat catcatgggc atttttggcg
    28981 caatgaatat ggcgaatatg gtctggcggc gcaaaaaacg ctggtggatg gtatccgcaa
    29041 agaggttgcg gctgaaggcg gtagcgtgct gctactttcc ggtggcgaca ttaacactgg
    29101 cgtgcccgag tctgacttac aggatgccga acctgatttt cgcggtatga atctggtggg
    29161 ctatgacgcg atggcgatcg gtaatcatga atttgataat ccgctcaccg tattacgcca
    29221 gcaggaaaag tgggccaagt tcccgttgct ttccgcgaat atctaccaga aaagtactgg
    29281 cgagcgcctg tttaaaccgt gggcgctgtt taagcgtcag gatctgaaaa ttgccgttat
    29341 tgggctgaca accgatgaca cagcaaaaat tggtaacctg gaatacttca ctgatatcga
    29401 atttcgtaag cccgccgatg aagcgaagct ggtgattcag gagctgcaac agacagaaaa
    29461 gccagacatt attatcgcgg cgacccatat ggggcattac gataatggtg agcacggctc
    29521 taacgcaccg ggcgatgtgg agatggcacg cgcgctgcct gccggatcgc tggcgatgat
    29581 cgtcggtggt cactcgcaag atccggtctg catggcggca gaaaacaaaa aacaggtcga
    29641 ttacgtgccg ggtacgccat gcaaaccaga tcaacaaaac ggcatctgga ttgtgcaggc
    29701 gcatgagtgg ggcaaatacg tgggacgggc tgattttgag tttcgtaatg gcgaaatgaa
    29761 aatggttaac taccagctga ttccggtgaa cctgaagaag aaagtgacct gggaagacgg
    29821 gaaaagcgag cgcgtgcttt acactcctga aatcgctgaa aaccagcaaa tgatctcgct
    29881 gttatcaccg ttccagaaca aaggcaaagc gcagctggaa gtgaaaatag gcaaaaccaa
    29941 tggtcgtctg gaaggcgatc gtgacaaagt gcgttttgta cagaccaata tggggcggtt
    30001 gattctggca gcccaaatgg atcgcactgg tgccgacttt gcggtgatga gcggaggcgg
    30061 aattcgtgat tctatcgaag caggcgatat cagctataaa aacgtgctga aagtgcagcc
    30121 attcggcaat gtggtggtgt atgccgacat gaccggtaaa gaggtgattg attacctgac
    30181 cgccgtcgcg cagatgaagc cagattcagg tgcctacccg caatttgcca acgttagctt
    30241 tgtggcgaaa gacggcaaac tgaacgacct taaaatcaaa ggcgaaccgg tcgatccggc
    30301 gaaaacttac cgtatggcga cattaaactt caatgccacc ggcggtgatg gatatccgcg
    30361 ccttgataac aaaccgggct atgtgaatac cggctttatt gatgccgaag tgctgaaagc
    30421 gtatatccag aaaagctcgc cgctggatgt gagtgtttat gaaccgaaag gtgaggtgag
    30481 ctggcagtaa tccgaaagtg ccggatgttt gcatccggca caatgcttaa tcgcggcggg
    30541 cgatatcagc aaatttggca tcgaggatct ttgccagatc gcctgccgcc agttcgatat
    30601 ccagtccgcg cttgccgccg gaaacataaa tagtggcaaa ttcttgtgcg ggggcgtcga
    30661 taatcgttgg cagacgtttt ttctgcccca gtgggctaat ccccccaacc aggtatcccg
    30721 tcgaacgctg cgcgaccatc ggatcggcca tctcaacttt cttggcaccc agcgcttttg
    30781 ctactttttt aagatccagt tgacctgcga ccggcgtaac ggccacggca aggtgtttca
    30841 tatcaccgtt cactgccacc agcagcgttt tgtagacctg atccggattc aaacctaatt
    30901 ttttgacgac ttcatcgcca aaattggttt cagccggatc gtgctcgtag gtatggattt
    30961 gaaacgaaat cttgtttttt tcgagtaatt taactgcggg tgtcatggta attttcctgc
    31021 cttaaacaaa aaaacgaagc aagcatacgc ctgatggcgg tccaaaaaaa agagtcatct
    31081 tgcctaagag tattggcagg atggtgagat tgagcgacaa tcgagttaca ccgtcgctta
    31141 aagtgacggc ataataataa aaaaatgaaa ttcctctttg acgggccaat agcgatattg
    31201 gccatttttt tagcgcaaca tttgcggcaa attcccttct ccatacaggt gtagtgcacc
    31261 gaccgcgacc acatatcgcc ccggcggcat ggcgcgtaat ttatcccgcc aggcgagatt
    31321 tcgctgatgc atcagcacat cgtacagcga ctgactgaac gtattgggca gcgttatatc
    31381 attattttgc ggcggtgcat tcagccacca gctcatcatt tgttgcagca accgtgcgtt
    31441 ggtatgccag tgggtcagcg tatcgtccag cagcgccagt cctttgtcag ggagctggag
    31501 caacatggca atctggtttt cagccccttc cagttcaatc acgggtttat gttgttgctt
    31561 cgccgcctgc aatagctggt aatcgatacc gtattctgcc cgcagcccca gtttttgtgc
    31621 ctgcgtcgcc tgaagaacca tcgcgatttg ccacagcggt tgggtagaaa agagtgaggg
    31681 agaaatgccc atctcctggc taatgtgctg caggttttgg agttgctcct cgctaatgcg
    31741 ctcttccagc gcctcgcagg caggcaaatt agcaaaaggc gtatcgctgg tggaaacatc
    31801 cgcctcgacg atcagcgcat cggcgttttt gagctttttg agcaaacggg tgggcagggg
    31861 agccatatcg tggctaccca tatgaatact gccaatcaga tgaaaatggc gattgccggg
    31921 gagggtgata tcgatggcag gccaggtgta atgattaccg cgcagcgcgg cccaaagcgt
    31981 ttttacccgg tacaacagat ccatacgacc tccctttgtg aaatatcatg ctagcgcgcg
    32041 gtgagggatg gcgcaaatgg agttggggac ttgtttaatg tgtttgtatg attcagtatg
    32101 ttcttgcatc gctattcaca gggaagcaac agttaaaaac catgaaacag gcaacaagaa
    32161 aaccgacgac acccggagat attcttctct atgaatatct ggaaccgctc gatttgaaaa
    32221 tcaatgagtt agcagagttg ctgcatgttc atcgtaatcg cgtcagtgca ctgatcaata
    32281 acaatcgtaa actcaccact gagatggcat tccgtctggc gaaagttttt gataccacag
    32341 tcgattttag gctaaacctc caggcggcag ttgatctttg ggaagttgaa aacaacatgc
    32401 gcacccagga agaattggga cggattgaaa cagtggctga atatttggca cgccgtgaag
    32461 agcgtgcaaa aaaggtcgcg taaggccaaa acatcgcttc atctcgtagg ccggataagg
    32521 cgttcacgcc gcatccggca tccgagcacc attgcctgat gcgacgctta cgcgtcttat
    32581 caggcctaca aacctgtgcc tgaaccgtag gccggataag gcgtttacgc cgcatccggc
    32641 agtcatgcgt cgatgccaaa tgcgccgccc taaagcagcg catccgcaat gacgtactta
    32701 ttccttcggt ttaaaccgca gcaaccggtt ggcgttactt actacggtaa ttgacgagag
    32761 cgccattgcc gctccggcaa ctaccgggtt aagcagtgtt ccagtaaacg gccacaaaat
    32821 accggcggcg accggaatac cgatgctgtt gtagataaag gcaccgagca ggttctgctt
    32881 catgttgcgc agcgttgcgc gggaaatcgc gagcgcatcc gcaacgccca tcaggctatg
    32941 gcgcatcagg gtaatcgccg cggtttcaat ggcaacatca ctgccgccac ccatcgcaat
    33001 gccgacatcc gcctgggcca gcgctggcgc gtcgttaatg ccgtcgccca ccattgccac
    33061 ctgacgtcct tcactttgca gacgtttgat cgcttcggct ttaccgtccg gcagcactcc
    33121 ggcaatcact tcatcaattc cagcttcttt ggcgatcgca ttggcggtgg ttgggttatc
    33181 cccggtcaac atcaccagac gatatcccgc tttatgcagg cgttgcagcg ccgccacgct
    33241 atcactacgc aacggatcgc gtacagccag cagggctact gctttcccgt caatcgccag
    33301 cagcacaggc gttgctcctt gcgatgcctg agctgaaata tcggcttcaa tcgctttagt
    33361 atcgacttgc tgatcattta acaacgcctg attacccagc aataacgcat gaccttctgc
    33421 ttcaccgctc acgcccagcc cgcgcaatgt gcggaaaccg ttgacctgcg gtagctgcat
    33481 atcgcctgct ttatcaagga tcgctcgtgc caatggatgg ctggaacctt gctccagcgc
    33541 cgccgccaga cgcagcgcct gcgcttcgtc aacatcagca aatgttttca ctgcgacaac
    33601 ctgcggcttc ccttcggtca gcgtcccggt tttatcgaac acgacggtat cgagtgtact
    33661 ggcgcgttgc agcgcgtcgg cgtcccgcac cagcacgcca aactcagccg cccgcccgac
    33721 gccggaaata atcgacatcg gcgtcgccag tcccagcgca cacgggcagg cgataatcag
    33781 caccgtagtg gcaatcacca gggtatagac aatctgcggt gccggaccaa agaaatacca
    33841 gattgccgca ctgacaagcg caataaccac tactaccggc acaaatatgg ctgagatttt
    33901 atccgccagc tgaccgattt ctggcttgct gctctgggcc tggcgcacca tgcgaatgat
    33961 ccttgatagc gtggtatggc tgccaaccgc actggcacga aacagcacac tgccgtcctg
    34021 taccactgtc ccggcatgga cgctatcgcc ttcgcctttt tgctgcggga ttggttcgcc
    34081 cgtcagcatc gcttcatcca gccatgcttc gccctgggta atctcgccat ctaccggcac
    34141 gcgatcgccg gtcgtcaggc gcagcaacat acctggctgc acttctgcca gaggcacgct
    34201 tttttcacct tcgtcagtaa ccaggcgcgc cgtcggtggg gttaaatcga gtaatttctc
    34261 cagtgcctta gaagaacgct gacgtgcgcg tgcttccagc atatggccga gattgatcag
    34321 accgataatc atcgcactgg cttcatagta aagatgtcgc gcttccatcg ggaaccactg
    34381 cggccacagg ttgacgctca tcgaatagag ccacgccacg cccgttccca gcgccaccag
    34441 cgtatccatc gtcgctgcac cgttcagcag gcttttccat gcactgcggt aaaaatggcc
    34501 gctggcgaaa accatcaccg ccagggttat caggccgata accaaccaca ggctgcggtt
    34561 gtcggcggtg actatcatgt tatcgccgat catcccccag accatcaccg ggatacccac
    34621 cgccagtgcg acaattgcct gccagcggaa gcgcttcatc gtagcgacgg cggtttcttg
    34681 ctggcgttcg cggcgtttcg cgtcatcttc aatcgcttcc gcgccgtagc ccgctttttc
    34741 caccgcctgc actaaatctt gtggggaggc actgcccatc accagcgcag tacgctccgc
    34801 caggtttacc cgtgcctgag tgacgcccgg tacgctttgc agcgcatttt gtacgcgggt
    34861 gacacagctg gcgcagctca tgccgctcag cagcaactgc tggctgtcat cgtcatcggc
    34921 ggtcgctgcc ggaagcgcct cagaaaccgc tgtcagtgct tccgacggga ttgatgactc
    34981 cgccagcggt ttagcctttg ggtggcttac agatgcgtca taacccgctt gtttgatggt
    35041 ttcgatcagc tgttctgcac tggcagtccc ggtaacgtgc gcttcagtga tagacacatc
    35101 cgcttgttcc acatccgggc gctgttcaag actttctttc acgcgtttaa cgcagtgacc
    35161 gcaggacagg ccgtccaggt tcaggtcgat agtttgtgac ataaaacact cctttaagac
    35221 agttttgact ggctgtgata aaggttaaac cttccagcaa ggggaaggtc aagaaattaa
    35281 taaaccaggc gggtaaaagt ccgtaaagat taaaaaatcg gctcgatttg catcaggatt
    35341 agacatttat ttctttgttt tcctgtagtt aagttgcggg tgctaagtta aatcaggatg
    35401 cctgaaaatc ggcactgggg tgaggaatta cctcccgcat ctataaaaag gagttaacaa
    35461 aagatgttag atgcaaacaa attacagcag gcagtggatc aggcttacac ccaatttcac
    35521 tcacttaacg gcggacaaaa tgccgattac attccctttc tggcgaatgt accaggtcaa
    35581 ctggcggcag tggctatcgt gacctgcgat ggcaacgtct atagtgcggg tgacagtgat
    35641 taccgctttg cactggaatc catctctaaa gtctgtacgt tagcccttgc gttagaagat
    35701 gtcggcccgc aggcggtaca ggacaaaatt ggcgctgacc cgaccggatt gccctttaac
    35761 tcagttatcg ccttagagtt gcatggcggc aaaccgcttt cgccactggt aaatgctggc
    35821 gctattgcca ccaccagcct gattaacgct gaaaacgccg aacaacgctg gcagcgaatt
    35881 ttacatatcc aacagcaact ggctggtgag caggtagcgc tctctgacga agtcaaccag
    35941 tcggaacaaa caaccaactt ccataatcgg gccattgcct ggctgctgta ctccgccgga
    36001 tatctctatt gtgatgcaat ggaagcctgt gacgtgtaca cccgtcagtg ctctacgctc
    36061 atcaatactg ttgaactggc aacgcttggc gcgacgctgg cggcgggtgg tgtgaatccg
    36121 ttgacgcata aacgcgttct tcaggccgac aacgtgccgt acattctggc cgaaatgatg
    36181 atggaagggc tgtatggtcg ctccggtgac tgggcgtatc gcgttggttt accgggcaaa
    36241 agcggtgtag gtggcggtat tctggcggtc gtccctggcg tgatgggaat tgccgcgttc
    36301 tcaccaccgc tggacgaaga aggcaatagt gttcgcggtc aaaaaatggt ggcatcggtc
    36361 gctaagcaac tcggctataa cgtgtttaag ggctgatcat gatgaacacg gaaggtaata
    36421 acggtaacaa acccctcggt ctatggaacg tcgtttccat cggtattggg gcaatggtgg
    36481 gggcggggat cttcgcgctg ctggggcagg ctgcgttgct aatggaagcc tcgacctggg
    36541 gcgcctttgc ttttggcggt attgtggcta tgttttccgg ttatgcctat gcgcgtctgg
    36601 gggcgagcta tcccagcaat ggcggtatta tcgacttttt tcgtcgcgga ttaggcaacg
    36661 gcgtcttttc gctagcgctc tcgttgctat acctgttgac gctggcggtg agcatcgcca
    36721 tggttgcccg tgcttttggc gcttatgccg tgcagttttt gcatgaaggc agccaggagg
    36781 agcaccttat tttgctctac gcgttgggga tcattgcggt gatgacgctt ttcaactcct
    36841 taagcaacca tgcggtaggg cggctggaag tgatcctcgt cggcattaaa atgatgatcc
    36901 tgttattgct gattattgcc ggtgtctggt cgctgcaacc ggcgcatatt tccgtctctg
    36961 cgccccccag ctccggtgcg ttcttctcct gtattgggat aaccttcctt gcctatgcgg
    37021 gctttggcat gatggcgaac gcggcggata aagtgaaaga tccgcaggtc attatgccac
    37081 gggcgtttct ggtggcgatt ggcgttacca cgttgcttta tatctcgctg gcgctggttt
    37141 tgcttagcga tgtatcggca ttagagttag aaaaatatgc tgataccgcc gtagcgcagg
    37201 ctgcttctcc gctgctcggg catgtgggtt atgtgatcgt cgtcatcggc gctttactgg
    37261 cgacggcttc aggcattaac gcgaacctgt tcgccgtgtt taacatcatg gacaacatgg
    37321 gcagcgaacg cgaactgccg aagctaatga ataaatccct gtggcggcag agtacctggg
    37381 gcaatatcat cgtcgtggtg ttgataatgc tgatgacggc ggcgctaaat ttagactcac
    37441 tcgccagcgt tgccagcgcc acctttttga tctgctatct ggcggtgttt gtggtggcga
    37501 tccgcctgcg tcatgatatt cacgcctcgt tgccgattct tatcgttggt acgttggtga
    37561 tgttgttggt gatcgttggc tttatctaca gcctgtggtc ccagggcagc cgtgcgttga
    37621 tatggattat tggtgcaatc ttactcagcc ttattgtggc aatggtcatg aagcgtaata
    37681 aaaccgtata acacctctct gtgcgcagta cttcctgtat tattgtggtg gcggtcgata
    37741 ttcgcactgg caaaaaaacg tgcttgaata tctgttgaaa ccctttaaca aagcacagga
    37801 ggcgttgcgc gaacgatgaa catcagcgat gtagcaaaaa ttaccggcct gaccagcaaa
    37861 gccattcgct tctatgaaga gaaggggctg gtgacgccgc cgatgcgcag cgaaaacggt
    37921 tatcgcacct acacgcagca gcatctcaac gaactgacct tactgcgcca ggcacggcag
    37981 gtgggcttta acctggaaga gagcggcgag ctggtgaatc tgtttaacga cccgcagcgg
    38041 cacagcgccg acgtcaaacg gcgcacgctg gagaaggtgg cggagatcga acgacacatt
    38101 gaggagctgc aatccatgcg cgaccagctg ctggcactgg cgaatgcctg ccctggcgat
    38161 gacagcgccg actgcccgat tatcgaaaat ctctccggct gctgtcatca tcgggcaggg
    38221 tgattaagac gagacggctc ggatatgtag ggttatccct tctatcgcaa tgacttcaac
    38281 atgcgtacct gcaccgagat cctcgctggc gctgacaggc catgaactgt caccgacgcg
    38341 catatgaccg cgcccgttga ccagcggaga ttccagcaca aaacgtcggc caatcagctg
    38401 ctgcccgcgc tggtttaaat gactatcgct gtgcttttgt tcgcgcaccc gccgcgacaa
    38461 ccatttccac cacagccagg cggcgagcag cgtcaggatg gcaaacatta ccccttgcca
    38521 ctcccaaccc agcggcacca gccagaccac caggccggta atcaccgctg ccacgccgct
    38581 ccacaacaaa taaccatttc cgcccagcat ctcggctgcc agcagcaacc cgccgagact
    38641 gagccagaaa atatgtggat gaacgaccat taactccatc atggctgagt ccgtttgttg
    38701 gcgctgtctt tcaccagctc ggcaatcccg gcaatcgacc ccatcaggct gctggcctct
    38761 aatggcatca tcactacttt gctgttactg gaggaaccga tctgctgtaa cgcttcggtg
    38821 tatttttgcg ctacgaagta gttcaccgcc tgaatatcac cggaggcgat agcttcagac
    38881 accattttgg tggcgcgggc ttctgcttcg gcggaacgtt cacgcgcttc agcctgtaaa
    38941 aacgccgact gacgttcgcc ttccgctttc aggatttgcg actgtttttc accttcggct
    39001 ttgaggattt ccgcctgacg gatcccttcc gcttcaagaa tgtaagcgcg tttggtacgt
    39061 tccgctttca tctgcgcgtt cattgaagag ataagctctg ccggtgggcg cacgtcgcga
    39121 atttcaatac gggtgacttt aatcccccac gggttggtgg cttcatcgac aatacgcagc
    39181 aggcgtgagt tgatgctgtc gcgctgagag agcatttcgt caagttccat tgaacccaac
    39241 acggtacgga tgttagtcat ggtcaggttg atgatcgcca gctccagatt gctgacttca
    39301 taggcagcgc gtggcgcgtc aatcacctga ataaaacaga ctgcgtcgat ggtaacgttg
    39361 gcgttatctt tcgagataac ttcctgggaa gggatatcga gcacttgctc catcatattg
    39421 atcttgcgac caatgcgatc cataaacggc accaccagac tgagccccgg ctgtaacgtt
    39481 ttggtatagc gaccaaaacg ttctaccgtc cattgatagc cctgcggcac gattttgacg
    39541 cccgcgccga caatgaccag cgcgacaaaa atgagaatcg ggataaagat aagcatcgga
    39601 aaaacctcct gttgtaccgt ccataatcag caaaattgct gcttgattaa acaaattata
    39661 cctgattact gaaagagagt tcccccttat tcctgcgaag gataaactgt ttttagtaaa
    39721 aatcagaaaa agggaacagc gatgcaggaa aatagtcctt tgcttcagct acaaaacgta
    39781 ggatatctgg cgggtgatac gaagattctt aataacatca atttttcgct gcgtgctggc
    39841 gaatttaagt taattaccgg tccttctggt tgtggcaaaa gtacgctgct aaaaatagtt
    39901 gcttcattga tcagcccaac cagcggaacg ttactgtttg aaggtgagga tgtcagcaca
    39961 ctaaaaccag aaatctaccg ccagcaagtc tcttactgcg cccagacgcc gacgctgttt
    40021 ggcgatacgg tatacgataa tctgatcttt ccctggcaga tccgtgacca gcagcctgac
    40081 ccggccattt ttctcgattt tctcgaacgc ttcgctttgc cggacagcat tttgacgaag
    40141 aatatcgccg agctatctgg tggtgaaaaa caacgcatct cattgattcg taacctgcaa
    40201 tttatgccga aggttttatt gctggatgaa ataaccagtg cgctggatga aagtaataaa
    40261 cataacgtca atgagatgat ccatcgttat gtgcgcgagc aaaatattgc cgtgctgtgg
    40321 gtgacacacg ataaagacga aattaatcat gcggataaag tgattacact gcaaccgcat
    40381 gccggagaaa tgcaggaagc acgctatgaa ctcgcataat attactaacg aatcattagc
    40441 actggcatta atgctggtgg tggtggcaat cttaattagc cataaagaaa aactggcgct
    40501 ggagaaagat attctatgga gcgtcgggcg agcgataatt cagctgatta ttgtcggcta
    40561 tgtgctgaag tatattttca gcgtggatga tgccagcctg acattattga tggtgttatt
    40621 tatctgcttt aatgcggcgt ggaacgcgca aaaacgcagt aaatatattg ctaaagcttt
    40681 tatctcatcg tttattgcca ttacggtcgg ggcgggaatt accctggcgg tgctgattct
    40741 ctcaggggcg attgaattta tcccgatgca ggtgatccct gtcgccggga tgattgccgg
    40801 taacgccatg gtagcggtgg ggttgtgtta caacaattta gggcaacggg tcattagcga
    40861 acagcaacag attcaggaga aactgagtct tggcgcgacg ccgaagcagg cttcagcgat
    40921 attgattcgc gacagtattc gcgcggcttt aattccgacg gtcgattcag caaaaacggt
    40981 tggcttagtg agtttaccag gaatgatgtc cgggctgata tttgccggga ttgatccggt
    41041 gaaggcgatt aaatatcaga ttatggtgac ctttatgctg ctctcaaccg ccagcttgtc
    41101 gaccattatt gcctgctatt taacctatcg taagttttat aattcgcgcc accagttggt
    41161 ggtgactcaa ttgaagaaga aatgatgcga tgccggatgc gcagcatcgc atccggcgtt
    41221 gtggttcatg tgccgaacaa cggaatatca atacaacaat gcatacagct ggcggcgata
    41281 cttcgacgcc agtgcatcac ccgtacccag cgcagcgagg atctcctgga acgttttacg
    41341 cgtctgaccg tctgcggcgg tgagatcttt acgcagatgc ccgaacagca actccagcgc
    41401 ctcttcattg cgcccaacct gatgcagttg cagcgccagt tgcgtcgcca gtgcggcatc
    41461 ttctggattc tccgccacct gctgttgcaa ctgttgaatt tccggcttat cagccgcctg
    41521 cttcagcagt tcgatttgcg ctaccagccc ctggtagcgg gtgtcctgat cctgcaacgg
    41581 aatggttttc agcaccgctt ccgcatcttc agaacggttc agcgcaatca gcgtttctgc
    41641 cagcagcagg ccgatctccc cgttctgatt cgacaactgc caggcgtctt tcagcaatgg
    41701 cagggcatcg gtgtaattgc cttcctgcat cagttgcatc gcctgctgcg ctttcagctc
    41761 ttcttcgcgc ggcagcactt tatccagcag ggcgcggatc gcctcttccg gttgcggccc
    41821 ctggaagcca tctaccggtt gcccgttctg gaacagatac acggtcggaa tcgcgcgcag
    41881 accaaactgg gcggcaatca tctgctccgc gtcgcagtcc agcttcgcca gaataaattg
    41941 cccgttgtac tgcgccgcga ggctttccag gatcggggtt aactgcaaac agtgctggct
    42001 acgttcagac caaaaataga acagcaccgg agtggtcatc gactgttcaa gaacctgttg
    42061 caggttagat tcgttaatgt tgacaatatt ttctacggac atggagtcgc tctctgttgt
    42121 cgatattttc tttgacatgg gggcttaagc gcgcgcttca actcacccct gcaatatttt
    42181 gtccatcacg cgcccaggca gcaggcgctc gagcaccatt actgcccagg tcaccagcgt
    42241 taccggatag cgcatcttcg gcttctcgct aataaaagca tggcgcactt tgtcgaccac
    42301 cgcttccggt cccaacgtaa agcgggcggc gatgccggga ttttcgactg gtttatcact
    42361 ttgcgtctgg ttgacgttgt cggtgaagcg agtacgaatg ggaccgggtt cgatcaggct
    42421 gactttaatt ccgctgtggc gtagctccat gcgcagcgcg tctgaccacg cctccagcgc
    42481 atatttactg gccgcgtaag cgccacgacc cggcgtggag attaatccca tcaccgatga
    42541 tgtcatcaca atacgccctt caccgtgcgg taacatcgcg ggtaacaggc gcatggtgag
    42601 ctggtgtgcg ccgaaaaagt tggcggaaaa ctgctgttcc atctgcgcac ggctgatggt
    42661 ggaaaggggg ccatacatgc cgaatccggc attgttaaag atcccataca gacaattatc
    42721 ggtcagggcg atcacctcgt ctgctgcgcg attaacactt tctggtgaat ccagatcgat
    42781 caacacgccg gtaaatccca tgttgttcat acgctcaaca tcattcggtt ttcggcaacc
    42841 tgccagcaca tgaaaaccct ggcgttttaa ttcgagcgcg ctttccaggc caattccact
    42901 ggaacatccg gtaattaaga ccgatttttg cataacttta cctgtcagga tctccgttgc
    42961 tttatgagtc atgatttact aaaggctgca actgcttcgc catccagtcg gcaataaacg
    43021 gctgggcgtc gcggttggga tgaataccgt catcctgcat ccattgtggc ttgaggtaga
    43081 cctcttccat aaaaaagggc agcagcggaa catcaaactc tttggcgagt ttggggtaaa
    43141 tggcgctaaa ggcttcatta taacggcgac catagtttgc aggcagacgt atttgcatta
    43201 acaatggttc agcgttggcg gctttgacat cctgcaaaat ctggcgcagc gtttgctcgg
    43261 tttgctgtgg ctgaaaacca cgcaaaccat cattgcgccc agttcaacca gcacccaacg
    43321 cggctgatgc tgtttgagca gagccggaag gcgcgccagc ccttgttgcg aggtgtcgcc
    43381 gctgatgctg gcattaacta ccgacgtttt actctgccac ttatcattca acaaggcagg
    43441 ccaggccgcg ctggcagaca ttcgataccc ggcgctcagg ctatcaccca gaatcaataa
    43501 cgtgtccgct gcggcggcac ggaaggttaa caggaccagg aacaggaagg gcaaatgcca
    43561 gcggaaaaca ttgttgaagt tcatcatctt aagaagtccg tcggtcaggg ggagcatgaa
    43621 ctctccatcc tcaccggagt tgagctggtt gtcaaacgtg gcgagaccat cgcactggtg
    43681 ggcgagtcgg gatcgggtaa gtcaaccttg ctggcgatcc tcgccgggct tgatgacggc
    43741 agcagtggcg aagtgagtct ggtgggacaa ccgctacata atatggacga agaagcgcgg
    43801 gcaaagttgc gcgcgaagca cgtcggcttt gtttttcagt catttatgtt aattcctacc
    43861 cttaacgcgc tggaaaacgt cgagcttccg gctctgctgc gcggtgagag tagcgcggaa
    43921 agtcgtaacg gggcgaaagc gttgctcgaa cagttagggc tgggtaaacg tctggatcat
    43981 cttccggcac agctttccgg cggtgaacag caacgagtgg cgctggcacg agcctttaat
    44041 ggtcgacctg atgtgctgtt tgccgacgaa cccaccggca accttgaccg ccagacgggc
    44101 gataaaattg ccgacctgct gttttccctc aaccgtgaac atggcaccac gttgattatg
    44161 gtgacccacg acctgcaact ggcggcacgc tgcgaccgct gcttacggct ggtgaacggg
    44221 cagttgcagg aggaagcatg attgcacgtt ggttctggcg cgaatggcgt tcgccgtcgc
    44281 tattaattgt ctggctggcg ctaagcctgg cggtggcctg cgtgctggcg ctgggcaata
    44341 tcagcgatcg catggagaag ggcttaagcc agcaaagccg tgagtttatg gcgggcgatc
    44401 gggcgttgcg cagttcacgc gaagtgccgc aagcgtggct ggaggaagcg caaaagcgcg
    44461 gcctgaaagt cggcaagcag ctgactttcg ccacaatgac ctttgcaggc gacacaccgc
    44521 agctggcgaa cgtcaaagcg gtggatgata tctacccgat gtatggcggt ctgcaaacta
    44581 atccccctgg cctgaaaccg caggcgggca gcgtattgct ggccccacgc ctgatggcac
    44641 tgcttaacct gaaaacgggc gacaccattg acgtgggcga tgccaccttg cggattgccg
    44701 gagaagtgat tcaggaaccg gattccggtt ttaacccctt ccagatggct ccgcgtctga
    44761 tgatgaatct ggcggatgtc gataaaaccg gagccgtgca gccggggagt cgggtcacct
    44821 ggcgttataa attcggcggc aacgagaacc agctcgacgg ctatgagaaa tggttgttac
    44881 ctcagcttaa acccgaacaa cgctggtacg gtctggaaca ggacgaaggc gcgctggggc
    44941 gatcgatgga acgctcgcaa cagttcctgc tgctttcggc gcttctgacc ttgctgctgg
    45001 cagtggcagc ggtggcggta gcgatgaatc attactgtcg cagtcgctac gatctggtgg
    45061 cgatcctcaa aacgctgggg gcagggcgag cgcaactgcg taagctaatc gtcggtcagt
    45121 ggttgatggt gctgacgctt tcagccgtta ccggtggggc cataggcctg ttgttcgaaa
    45181 acgtgttgat ggtgctgctc aagcccgttc tgcctgctgc actaccgcca gccagcctct
    45241 ggccgtggct gtgggcgctt ggcaccatga cggtcatctc gctgctggtg gggctacgac
    45301 cataccgctt gttgctggca acgcagcctt tacgcgtatt acgtaatgat gtggtcgcga
    45361 acgtctggcc gctgaagttt tatctgccga ttgtcagtgt ggtggttgtg ctgctgctcg
    45421 ccggattaat gggtggcagc atgctgcttt gggcggtgct ggcgggcgcg gtagtactgg
    45481 ctttgctgtg cggtgtgctg ggctggatgc tgctgaatgt acttcgccgc atgacgctga
    45541 aatcgctgcc tctgcgcctg gcggttagcc gcctgttacg tcagccgtgg tcaacgttaa
    45601 gccagctttc ggcattttcg ctctccttta tgctgctggc actgctgctg gtgctgcgtg
    45661 gcgatctgct cgaccgctgg cagcagcagc taccgccaga aagcccgaac tactttttaa
    45721 ttaacatcgc cacagaacag gtagcaccgc taaaagcgtt cctcgcggaa catcatatag
    45781 tcccggaatc attttatccg gtagtgcggg cgcggctgac ggcgattaat gataagccga
    45841 cagaaggtaa tgaagatgag gcgcttaatc gcgaactcaa tcttacctgg caaaatacgc
    45901 ggcccgatca taatccgatt gtcgccggta actggccgcc aaaagccgat gaagtgtcga
    45961 tggaagaggg gctggcgaaa cgcttaaacg ttgccctcgg tgataccgtg acttttatgg
    46021 gcgataccca ggagttccgc gctaaagtga ccagcctgcg caaagtggac tgggaaagtc
    46081 tgcggcctaa tttctatttt attttccctg aaggggcatt agacgggcaa ccgcagagct
    46141 ggcttaccag tttccgctgg gagaatggca acggcatgtt gacgcaactc aaccgccagt
    46201 tcccgaccat tagcctgtta gacattggcg cgattttaaa acaggtcggt caggtgctgg
    46261 agcaggtaag tcgggcgctg gaggtgatgg tggtgctggt caccgcctgc ggtatgttgc
    46321 tgttgctggc acaggtgcag gtgggaatgc gccagcgtca tcaggagctg gtggtatggc
    46381 gcacactcgg tgcggggaaa aaactgctgc gcacgacatt gtggtgtgat gttcgccatg
    46441 cttgggtttg tttccggcct ggtggccgca attggtgcgg aaacggcact ggcagtgttg
    46501 caggcgaaag tgtttgattt cccgtgggag ccagactggc gattgtggat tgttctgccg
    46561 tgcagcggtg cgctgctgct gtcgcttttc ggcggctggc tgggtgcgcg actggttaag
    46621 ggtaaggcgc tgttcaggca gtttgtgggg tgatgacagg tgttagattg cctgatgcgc
    46681 tacgcttatc aggcctacga agagcgtgca atatattgat tttgcaagat tgtgtaggcc
    46741 ggataaggcg tttacgccgc atctggcatg aacagtgtgc attttggcaa taaatatgaa
    46801 accaactgtc atgtcggttt ttttaaaggc cgaaggaggt aaaccttcgg ccttagctac
    46861 gtttcctcag ttattcaata aactgacgga tcagttccag cagcgcgatt agcgccttaa
    46921 gaacgatgag aactttatcg agaaaagttt tcatccgttg ctcccttaag ggagaaagac
    46981 aatcacagct tacccttacg ttgcctataa ctatgatttt cttcatgtgc tagatgagga
    47041 ctgtctctct ccaaattaga gcaccagaat cagcaccacc tgtatcctga ccaggactta
    47101 aaagtacttt tccgcactgc ggtaatccct tgaggcagta tcactactag cgtcgggatt
    47161 gtatcgccaa catagctgtt tgaatatgta ggcatcctga cttataattc acttgctaga
    47221 tgaggactgt ttaatttctg cccggaacca ccaagtcttg ggcggaaaaa ataaaaaaac
    47281 cgactgtcat gtcggttttt ttatatctgt attctggtgc aaccattttg ttctcacgac
    47341 aatcgccgtt tcagaatacc gcccatcact gcgccttaac ccataccgcc acttccgccc
    47401 actcaccgcg aaagacaact ttttccgctt ttttctcaag ctgatagcga tacattgggt
    47461 cgtaatattc tttaagtaac ggcaccagcc aggccagatg accgtcggtg ctgccggtgg
    47521 tgagttgcgt tgtcagcgcc gtatccagct gggcagcgag ttcgttatag cgctgtaacc
    47581 ccagccgacg cttaatcgcc gaaagtccgt gatgcaggta ttcgcaatat tcctgccagc
    47641 cctgttcgtc gccataagcg tgggtaaaat catgatgcat acgcaagaaa tactcttcat
    47701 tcaggcgttc aagacggatc tcaaacggat cttctaccac cgcaatcgcc gcctgagtca
    47761 ttcgctcgcg cagacattcc ggcaggtgat tcgaaccgat catccggctt tcatcttcca
    47821 gcacccacag gcgcaaatcc tgacgggcgt cggtttttag catttcggca gccagcaggt
    47881 tttcaaaact cgcctggcta agttgtggtt gtaacgtgcg accaaacgcc gaaccgcgat
    47941 gacgcgccag cccttccaga tcaacaccgt ttggctgttg ttgcactaat agcgttttac
    48001 cgctgccggt acaaccgcca atcagcacta tcggtttttg tgccagttca atggtcgcct
    48061 gaatcgcggt ctggcgcagt gccttataac cgccttccac cagcggataa tcaatccccg
    48121 cttcatgcag ccagcgttgc acaatatgtg aacgttgacc gccacgggcg cagcagagaa
    48181 taccttgcgg attttgcagg cacgctgccc gccaggcgta catacgctgc tgacgaattt
    48241 cacccgccac cagtttatgc cccagcgcca gcgctgcgtc tgagccttgc tgtttatagc
    48301 aggtgccaac ggaggcgcgt tcatcgttat ccattaacgg cagattgata gcggcgggca
    48361 ttgcgccttg ctcaaactcg ataggggcgc gaacatcaat aatgggcgta tcagcaatca
    48421 gcagggcacg atagtcctgt tccgtgtgtc tctcttgcat agttaaaagt gaacctcaaa
    48481 tcagcttgcg cgctatttta cgcgccaacg cgcaaggaaa cttgattttt aactgcgtgg
    48541 ggtgccgaaa atttctaaaa atccgctgat ttccggcctg tgttgggtaa acaaggtcac
    48601 aatatcttct accgctttgc agctgccaaa tttgcgccat gccagactca atggcgaagg
    48661 agggcgcatc gttgggatta cccggctgac cagttgttga ttatcgatca ttgactggca
    48721 aagtgatttt ggcaaaaaac caatgccaac acccgccaga tgggcggcga ttttcgtttc
    48781 catatcggga acaataatct ctttttgccc cggcaatcgc caggcgacgc gtttggttaa
    48841 ggtgccggcg ctgtcttcaa tattgaccgc cggaaagcga cgcaactgcg cttctgttag
    48901 cggctcttca acgttcgcca gcggatgatc cgccgccatg acaaatcgcc attgcaccga
    48961 tcctaaggga tcaagactaa aggtatttgc cagcgcctca gttcccgtga ccccgatagc
    49021 cagcgaaaaa ccttcgtaca atagcgagtc ccagacgccc atatagattt gtcgggagat
    49081 gtgaaactgg gtaaaggggt aacgctcatt cagccacgcc agcaactggg cgacggcctg
    49141 ggggttatag agcaggttgt tgatgacaat attcacctgg cgttccacgc catcattcac
    49201 ctgttgcagc tcgctgggca tactttccag ccagctcagc cagtctctgg cctgggaaag
    49261 tagatgctcg ccagccgctg tcaacgttac gctacgagtc gtacggaaaa acagcgctac
    49321 tccggtattc tcttccagaa gtttaatgcg ataactgatc gtcgccgtgg ttttacataa
    49381 tcgttctgcc gcttttgaaa aacttcctgt ttcagcaacc gcaatgaaag tccgcaaggt
    49441 ttctggatcg aacatcttca ggtatcccct tttaaatccg caagttgcgt gattttctta
    49501 tcctctgatt tatcagtatt tttacatgat aatcctgttc aatttgtgga ctaaatctag
    49561 ttttggaaaa acattccaac ttttgtatta atgttgttct cttaaggttt tagattgcct
    49621 gttattgaaa ccaagctgac cggtcggcgg tggttgaacg gaattatgtt acaaggacaa
    49681 aaagatgaaa cttcaggtat taccgttaag tcaggaagcc tttagtgctt atggcgacgt
    49741 aatcgaaacg cagcaacggg attttttcca tattaacaat ggcctggtgg agcgttacca
    49801 cgatttggcg ctggttgaga ttcttgagca agaccgtacg cttatcagca ttaaccgcgc
    49861 gcaaccggcg aatctgccgc tgaccattca cgaactcgaa cgtcatccgc tgggcattca
    49921 ggcctttatc ccaatgaaag gtgaagtgtt tgtggtggtc gtggcgttag gcgacgacaa
    49981 accagacctg tcaacgctgc gggcgtttat caccaacggt gaacaggggg tgaattacca
    50041 tcgtaacgtc tggcatcacc cacttttcgc ctggcagcgc gtcaccgatt ttctgaccat
    50101 cgatcgcggc ggcagtgaca actgtgatgt tgaaagtatt cctgaacagg aactctgttt
    50161 tgcgtgacgc ctgcaaccga cttgcataag ataaactaat tgttcattgt ttatgctcac
    50221 ttgtaggtcg gagttaacgt aggtatgacg gaagttagac ggcgcggcag gccaggacag
    50281 gcggagcctg tggcacagaa gggcgcacag gcgttagagc ggggaattgc gattctgcaa
    50341 tatctggaaa aaagtggggg aagttcgtcg gttagcgata tttctctcaa tctggatttg
    50401 ccgctctcca cgacctttcg cttgctgaag gttttacaag cagcggattt tgtctatcag
    50461 gacagtcaat taggctggtg gcatatagga ttaggtgtct ttaacgtcgg tgcggcgtac
    50521 atccataacc gcgatgtcct ctccgtcgcc gggccgttta tgcgccgcct gatgttactt
    50581 tccggcgaaa cggtcaatgt cgcgatccgt aacggcaatg aagcggtatt aattggtcag
    50641 ttagagtgta aatcgatggt caggatgtgt gcgccactgg gcagtcgtct gccgctgcat
    50701 gcttccggtg cgggcaaagc gctgctttat ccgctggcgg aagaggagtt gatgagcatc
    50761 attctgcaaa ccggtttgca gcagtttacg ccaactacgc ttgtggatat gcccaccttg
    50821 ctgaaggacc tggaacaagc gcgtgaactg ggctataccg tagataaaga agagcatgtt
    50881 gtaggtctga attgcatagc ttcagcaatt tacgatgatg tcggtagagt tgttgccgct
    50941 atctccatct ccgggccttc atcaagactg acagaagatc gttttgtcag tcagggtgag
    51001 ctggtcagag acaccgcccg cgatatcagc acggcgttgg gactgaaagc acatccatag
    51061 tgtctgtcgc atcccgctct gcggagcggg tttttttgac aaaatttgaa agttggaaaa
    51121 tttttccaac taatagaggt aggaataaaa tggcaaaaat gagagccgtt gacgcggcaa
    51181 tgtatgtgct ggagaaagaa ggtatcacca ccgccttcgg cgttccggga gctgcaatta
    51241 atccgttcta ctcagcgatg cgtaagcacg gcggtattcg tcacattctg gcgcgtcatg
    51301 tggaaggtgt ttcgcacatg gcggaaggtt atacccgcgc aacggcaggg aatatcggcg
    51361 tatgtctggg gacttccggt cctgcgggca cggacatgat caccgcgctc tattccgctt
    51421 ctgctgattc cattcctatt ctgtgcatta ccggccaggc accgcgcgcc cgtctgcata
    51481 aagaagattt tcaggccgta gatattgaag caattgctaa accggtcagc aaaatggcgg
    51541 ttacagttcg tgaagcggcg ctggtgcctc gcgtgctgca acaggcattt cacctgatgc
    51601 gttctggtcg tccgggtccg gtactggtgg atttaccgtt cgacgttcag gttgcggaaa
    51661 tcgagtttga tcctgacatg tacgaaccgc tgccggtcta caaacctgct gccagccgta
    51721 tgcagatcga aaaagctgta gaaatgttaa tccaggccga acgtccggtg attgttgccg
    51781 ggggcggggt aatcaatgct gacgcagctg cactgttaca acagtttgct gaactgacca
    51841 gcgttccggt gatcccaacg ctgatgggct ggggctgtat cccggacgat catgaactga
    51901 tggccgggat ggtcggtctg caaaccgcgc atcgttacgg taacgcaacg ttgctggcgt
    51961 ccgacatggt gtttggtatc ggtaaccgtt ttgctaaccg tcataccggt tcggtagaga
    52021 aatacaccga agggcgcaaa atcgttcata ttgatattga gccgacgcaa attggtcgcg
    52081 tgctgtgtcc ggatctcggc attgtctctg atgctaaagc ggcgctgaca ctgctggttg
    52141 aagtggcgca ggagatgcaa aaagcgggtc gtctgccgtg tcgtaaagaa tgggtcgccg
    52201 actgccagca gcgtaaacgc actttgctgc gcaaaaccca cttcgacaac gtgccggtga
    52261 aaccgcagcg cgtgtatgaa gagatgaaca aagcctttgg tcgcgatgtt tgttatgtca
    52321 ccactattgg tctgtcacaa atcgctgcgg cacaaatgct gcatgtcttt aaagaccgcc
    52381 actggatcaa ctgtggtcag gctggtccgt taggctggac gattccggcg gcgctggggg
    52441 gttgtgccgc tgatccgaaa cgcaatgtgg tggcgatttc tggcgacttt gacttccagt
    52501 tcctgattga agagttagcc gttggcgcgc agttcaacat tccgtacatc catgtgctgg
    52561 tgaataacgc ctatctcggc ctgattcgtc agtcacaacg cgcttttgac atggactact
    52621 gcgtgcaact cgctttcgag aatatcaact ccagtgaagt gaatggctac ggtgttgacc
    52681 acgtaaaagt agcggaaggt ttaggttgta aagctattcg ggtcttcaaa ccggaagata
    52741 ttgcgccagc ctttgaacag gcgaaagcct taatggcgca atatcgggta ccggtagtcg
    52801 tggaagttat tctcgagcgt gtgaccaata tttcgatggg cagcgaactg gataacgtca
    52861 tggaatttga agatatcgcc gataacgcag cggatgcacc gactgaaacc tgcttcatgc
    52921 actatgaata agggagataa atgatgttac gtttctctgc taatttatcg atgttatttg
    52981 gtgaatatga ttttctcgcc cgttttgaga aagctgcgca gtgtggtttt cgcggcgttg
    53041 aatttatgtt tccttatgac tacgacattg aagaattaaa acaggtactg gcgagtaata
    53101 aactcgaaca tacgctgcac aatttaccgg caggtgactg ggcggcgggc gagcgcggta
    53161 ttgcctgtat tcctggccgt gaagaagagt ttcgggatgg cgtagcagca gcgattc
//
LOCUS       DAJTUI010000018        52600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTUI010000018 DAJTUI010000000
VERSION     DAJTUI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.018"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..357
                     /locus_tag="OO464_001148"
     CDS             <1..357
                     /locus_tag="OO464_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR6226373.1"
                     /translation="NYQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILK
                     NELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNV
                     SSGQIMSRVKIVVMYN"
     gene            complement(395..1294)
                     /gene="nhaR"
                     /locus_tag="OO464_001149"
     CDS             complement(395..1294)
                     /gene="nhaR"
                     /locus_tag="OO464_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6226374.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1360..2526)
                     /gene="nhaA"
                     /locus_tag="OO464_001150"
     CDS             complement(1360..2526)
                     /gene="nhaA"
                     /locus_tag="OO464_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6226375.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3112..3264
                     /gene="mokC"
                     /locus_tag="OO464_001151"
     CDS             3112..3264
                     /gene="mokC"
                     /locus_tag="OO464_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR6226376.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3368..4498)
                     /gene="dnaJ"
                     /locus_tag="OO464_001152"
     CDS             complement(3368..4498)
                     /gene="dnaJ"
                     /locus_tag="OO464_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6226377.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4587..6503)
                     /gene="dnaK"
                     /locus_tag="OO464_001153"
     CDS             complement(4587..6503)
                     /gene="dnaK"
                     /locus_tag="OO464_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6226378.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6880..7284
                     /locus_tag="OO464_001154"
     CDS             6880..7284
                     /locus_tag="OO464_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR6226379.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7310..8023
                     /gene="msyB"
                     /locus_tag="OO464_001155"
     CDS             7310..8023
                     /gene="msyB"
                     /locus_tag="OO464_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6226380.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8172..8738
                     /gene="satP"
                     /locus_tag="OO464_001156"
     CDS             8172..8738
                     /gene="satP"
                     /locus_tag="OO464_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6226381.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8773..9360)
                     /gene="mog"
                     /locus_tag="OO464_001157"
     CDS             complement(8773..9360)
                     /gene="mog"
                     /locus_tag="OO464_001157"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR6226382.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9475..10428)
                     /gene="tal"
                     /locus_tag="OO464_001158"
     CDS             complement(9475..10428)
                     /gene="tal"
                     /locus_tag="OO464_001158"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6226383.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10707..12137
                     /locus_tag="OO464_001159"
                     /pseudo
     CDS             10707..12137
                     /locus_tag="OO464_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12207..12983
                     /gene="yaaA"
                     /locus_tag="OO464_001160"
     CDS             12207..12983
                     /gene="yaaA"
                     /locus_tag="OO464_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6226384.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13136..13432)
                     /locus_tag="OO464_001161"
     CDS             complement(13136..13432)
                     /locus_tag="OO464_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6226385.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13646..14932)
                     /gene="thrC"
                     /locus_tag="OO464_001162"
     CDS             complement(13646..14932)
                     /gene="thrC"
                     /locus_tag="OO464_001162"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6226386.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14933..15865)
                     /gene="thrB"
                     /locus_tag="OO464_001163"
     CDS             complement(14933..15865)
                     /gene="thrB"
                     /locus_tag="OO464_001163"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6226387.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15867..18329)
                     /gene="thrA"
                     /locus_tag="OO464_001164"
     CDS             complement(15867..18329)
                     /gene="thrA"
                     /locus_tag="OO464_001164"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6226388.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18355..18474)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18410..18475)
                     /gene="thrL"
                     /locus_tag="OO464_001165"
     CDS             complement(18410..18475)
                     /gene="thrL"
                     /locus_tag="OO464_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6226389.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18689..19375)
                     /locus_tag="OO464_001166"
     CDS             complement(18689..19375)
                     /locus_tag="OO464_001166"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6226390.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19775..19915)
                     /locus_tag="OO464_001167"
     CDS             complement(19775..19915)
                     /locus_tag="OO464_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226391.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20011..20727
                     /gene="arcA"
                     /locus_tag="OO464_001168"
     CDS             20011..20727
                     /gene="arcA"
                     /locus_tag="OO464_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6226392.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20786..22138)
                     /gene="creD"
                     /locus_tag="OO464_001169"
     CDS             complement(20786..22138)
                     /gene="creD"
                     /locus_tag="OO464_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6226393.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22196..23620)
                     /gene="creC"
                     /locus_tag="OO464_001170"
     CDS             complement(22196..23620)
                     /gene="creC"
                     /locus_tag="OO464_001170"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6226394.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23620..24309)
                     /gene="creB"
                     /locus_tag="OO464_001171"
     CDS             complement(23620..24309)
                     /gene="creB"
                     /locus_tag="OO464_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6226395.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24322..24795)
                     /gene="creA"
                     /locus_tag="OO464_001172"
     CDS             complement(24322..24795)
                     /gene="creA"
                     /locus_tag="OO464_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6226396.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25006..25875
                     /gene="robA"
                     /locus_tag="OO464_001173"
     CDS             25006..25875
                     /gene="robA"
                     /locus_tag="OO464_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6226397.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25872..26519)
                     /gene="gpmB"
                     /locus_tag="OO464_001174"
     CDS             complement(25872..26519)
                     /gene="gpmB"
                     /locus_tag="OO464_001174"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6226398.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26571..27091
                     /gene="yjjX"
                     /locus_tag="OO464_001175"
                     /pseudo
     CDS             26571..27091
                     /gene="yjjX"
                     /locus_tag="OO464_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27176..27502)
                     /gene="trpR"
                     /locus_tag="OO464_001176"
     CDS             complement(27176..27502)
                     /gene="trpR"
                     /locus_tag="OO464_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6226399.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27592..29529)
                     /gene="sltY"
                     /locus_tag="OO464_001177"
     CDS             complement(27592..29529)
                     /gene="sltY"
                     /locus_tag="OO464_001177"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6226400.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29740..31407
                     /gene="ettA"
                     /locus_tag="OO464_001178"
     CDS             29740..31407
                     /gene="ettA"
                     /locus_tag="OO464_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6226401.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEPPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31514..31681)
                     /locus_tag="OO464_001179"
     CDS             complement(31514..31681)
                     /locus_tag="OO464_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226402.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31715..32947)
                     /gene="nadR"
                     /locus_tag="OO464_001180"
     CDS             complement(31715..32947)
                     /gene="nadR"
                     /locus_tag="OO464_001180"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6226403.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32968..34350)
                     /gene="radA"
                     /locus_tag="OO464_001181"
     CDS             complement(32968..34350)
                     /gene="radA"
                     /locus_tag="OO464_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6226404.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34399..35367)
                     /gene="serB"
                     /locus_tag="OO464_001182"
     CDS             complement(34399..35367)
                     /gene="serB"
                     /locus_tag="OO464_001182"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6226405.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35473..36117
                     /locus_tag="OO464_001183"
     CDS             35473..36117
                     /locus_tag="OO464_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6226406.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36145..37160
                     /gene="lplA"
                     /locus_tag="OO464_001184"
                     /pseudo
     CDS             36145..37160
                     /gene="lplA"
                     /locus_tag="OO464_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37192..37455
                     /locus_tag="OO464_001185"
     CDS             37192..37455
                     /locus_tag="OO464_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6226407.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37616..38335)
                     /gene="deoD"
                     /locus_tag="OO464_001186"
     CDS             complement(37616..38335)
                     /gene="deoD"
                     /locus_tag="OO464_001186"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6226408.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38392..39615)
                     /gene="deoB"
                     /locus_tag="OO464_001187"
     CDS             complement(38392..39615)
                     /gene="deoB"
                     /locus_tag="OO464_001187"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6226409.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39667..40989)
                     /gene="deoA"
                     /locus_tag="OO464_001188"
     CDS             complement(39667..40989)
                     /gene="deoA"
                     /locus_tag="OO464_001188"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6226410.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41116..41895)
                     /gene="deoC"
                     /locus_tag="OO464_001189"
     CDS             complement(41116..41895)
                     /gene="deoC"
                     /locus_tag="OO464_001189"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6226411.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42153..43694
                     /locus_tag="OO464_001190"
     CDS             42153..43694
                     /locus_tag="OO464_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6226412.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43666..44529
                     /locus_tag="OO464_001191"
     CDS             43666..44529
                     /locus_tag="OO464_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6226413.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44743..45525)
                     /locus_tag="OO464_001192"
     CDS             complement(44743..45525)
                     /locus_tag="OO464_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6226414.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45522..46595)
                     /locus_tag="OO464_001193"
     CDS             complement(45522..46595)
                     /locus_tag="OO464_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6226415.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46717..46878)
                     /locus_tag="OO464_001194"
     CDS             complement(46717..46878)
                     /locus_tag="OO464_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6226416.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46995..47600)
                     /gene="osmY"
                     /locus_tag="OO464_001195"
     CDS             complement(46995..47600)
                     /gene="osmY"
                     /locus_tag="OO464_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR6226417.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47993..49582)
                     /gene="prfC"
                     /locus_tag="OO464_001196"
     CDS             complement(47993..49582)
                     /gene="prfC"
                     /locus_tag="OO464_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6226418.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49673..50350)
                     /gene="yjjG"
                     /locus_tag="OO464_001197"
     CDS             complement(49673..50350)
                     /gene="yjjG"
                     /locus_tag="OO464_001197"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6226419.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50365..50811)
                     /gene="rimI"
                     /locus_tag="OO464_001198"
     CDS             complement(50365..50811)
                     /gene="rimI"
                     /locus_tag="OO464_001198"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6226420.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50780..51193)
                     /gene="holD"
                     /locus_tag="OO464_001199"
     CDS             complement(50780..51193)
                     /gene="holD"
                     /locus_tag="OO464_001199"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6226421.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51296..52327
                     /gene="rsmC"
                     /locus_tag="OO464_001200"
     CDS             51296..52327
                     /gene="rsmC"
                     /locus_tag="OO464_001200"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6226422.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12527 a        14235 c        13181 g        12657 t
ORIGIN      
        1 aattatcagt ttcatcatat aagatttgac tgcatccagg gcgagcaagg tgaattagtt
       61 tttgaaccca gtgacgttga atattacttt gaaccagtga cgatacagga gtcaggaacc
      121 agcatattaa aaaatgagtt agaaaacagt gaggacggtg cgggtcaaat aggctttcaa
      181 ctctccaatg atggtacgaa tgaaatccag tatggtaaaa gcaattacta ctcttttcag
      241 catccccatg aaggaagtaa tcagattcct ctatttattc gcccgcgtac atatggaaac
      301 aatgtctcaa gcgggcaaat aatgagccga gtcaaaatcg tggtaatgta caactaatct
      361 atctccccca ccttaacttt gggggagctg ccgattaacg caccgctgga ctaaaaagcg
      421 cagaataatc cgtattgcag attcgctgta ccgccgggtg ctgaatcatc cgctcagcaa
      481 aaatagcatg gtactcttcc atcacattct cgacgcgacc aatttctacg acagttttat
      541 cggcataaaa gtcatatgca taaagcgttg gggcaacgaa gattgcattg tgcatcgcac
      601 caaaagcttt catcaaagcg gcatcatcaa actcgccgag gatttctacg tttaatccct
      661 gggagttaaa ccagttaagc aatttgcgcc ctaacattga acgtcgccca ggaatcaaaa
      721 gtcgccgttc ttccagacaa gccgggaacg gtttttctgg tggtggattt gtacaccaga
      781 aactcacgcc acattcgcca attctcacgg agaacaggcc ttcctgctgc gtagagtcta
      841 tcggacagtc agaaatgatc atatccagtt tatgctgact taattgctcc agcagcattt
      901 cgtgggtgga ttcgaagcag cgaagatgaa tgggctcgcc ttctaccact gcggcgttaa
      961 gtacgctact gaccaggcgt ttggaaagtg catcagccac gccaacgtca aacaataaat
     1021 tggattcttt gcgatagttc acaatatcca gcatttcctg gcttaaggtg aacattttat
     1081 cggcatagcg atagaccagt tctcccagct cgctgggttc gagaccacgt cccttgcgtt
     1141 taaataattt gccttgcagg cgctcttcca gcgctcgaat ctgtccggta atggtttgtg
     1201 gagttaaata aagcgcctcc gctgcgccaa ccacggaacc ttctttatag acatgccaga
     1261 agtaatacaa gtggttgtaa ttgatatgag acatgctcat ttctctccct gataacaatg
     1321 aaaagggagc cgtttatggc tccccggtaa accgtcctgt caaactgatg gacgcaaacg
     1381 aacgcgtaac cagctgtatc caattaccgc cgaagagata gaaccgacca ggataccgag
     1441 tttcgcccag ttaatcagtt ctggatctac gctaccaaag gccaggctgg caataaagat
     1501 agacatagta aaaccgatac cgcacaggat ccccaccacc ataatttgct gataagtcgt
     1561 tccctcaggc agatgcgcca gtttcaaacg cagcgccaac cagcagaaca gactaatccc
     1621 cagcggtttg ccaatcagca agccagcgat gatccccaat ggcagaatgg aggtcaagcc
     1681 atccagcgtg acgccttgca gtgaaacgcc agcattagca aatgcaaaca gcggcaaaat
     1741 cagatacgcc acccacgggt gcaacacatg ctccagtcgc ttcgctggag aacgcccatg
     1801 cttctctttc aaaggaataa agaagccgac aattaccccc gccagagttg cgtgaacccc
     1861 cgatttcaac accgcagtcc acaacaccac gccaacaaga atatagacgc ccgtgcggcg
     1921 tacaccacac agattcaata ccgcgagtac cgcaattgct acagccgcga cgccaaggga
     1981 ggccatcgat aagtcattag tgtagaacaa tgcgatgata atgatggccc caagatcgtc
     2041 gataatagcc agagccatca aaaagatctt cagcactaac ggaacacgac ttcccaacag
     2101 cgccagtaca ccaagtgcaa aagcaatgtc agtagccgcc gggatcgccc acccttcgcg
     2161 ggtaatcgga tcggcatagt taaaagccag atagagtaat gccggcacaa tcatcccacc
     2221 aatagcggcg ataactggaa atgcggcctg gcgtaagctg gctagcgatc cttgcatcag
     2281 ttcacgttta acttccagac cgactaacag gaaaaatacc gccatcagcg catcatttat
     2341 ccataacagc atgtttttgt tgatttcgag tgaaccaacc cggagctgaa ccggcgtctc
     2401 cagaaagtcg tgataccatc cactggttgc gccgctgttg gccataatca tcgccaggat
     2461 agcggcaatg ataagaataa tgcctcccga ggcatcactg ctaaagaatc gatgcagatg
     2521 tttcactttt tatttctctt tcaggtgaat agatcgtttt tactatttta ccccgcacga
     2581 atcatcgtta aaatcagatt atagtgtagg aataattcgt tttttacgag ccattgaaga
     2641 atttgcgcca tcagcggtga gatgcgtttt tgacggctgt aagcgctcac gagtgaccct
     2701 aagcccagat aatcatagct taaggtgagg attcctgagg gattaattag gaaaagtaat
     2761 aattaatttg ttgtcagtcg gcgatgcttg ttaggccaat atttcaaaaa taatccagct
     2821 tgtcgtcaat aattgcgatg aagagataaa ttaaagttaa ataattaaat cattaaaaaa
     2881 ttacgccaca aaaatgcgag gcgtcttcag gattagaatc gataaaacaa aatgggttaa
     2941 agagagcgat ataatagcgg cgggtgcttg aggctgtctg tctcaggcat tagctgaacg
     3001 gcagatagag aaaagccccg agtgatattt taccatcaac ccgaggcctc ctatatgctg
     3061 aacacatgta gagtgcctct tactgaccgt aaggtcaagg agaagagagc aatgaagcag
     3121 cataaggcga tgattgtcgc cctgatcgtc atctgtatca ccgccgtagt ggcggcgctg
     3181 gtaacgagaa aagacctctg tgaggttcac atccgaactg gccagacgga ggttgctgtt
     3241 ttcacggctt acgaatccga gtaagagcaa cggcggggag tgatccccgc cactttacag
     3301 gtgctcgcat atcttcaacg caccctattt ttacccaggc ctgcccacgg gcaggctttt
     3361 ggggaggtta gcgggtcagg tcgtcaaaaa acttcttcac accatcaaag aagctctttg
     3421 agcgcgggct gttgtgctcg ccggttgggc caccgaagct ttcttgcagc tcttgcagca
     3481 gctgtttctg cttttcgttc aggcctaccg gtgtttcgac gacaacgcgg cacagcaaat
     3541 caccctgtgc gccaccgcgg acagacttga cgcctttacc gcgcatacgg aatagcttac
     3601 cggtctgggt ttcgccaggc actttcagtt tgacgcgacc atcaagggtc ggtacttcga
     3661 tttcgccacc cagcgccgcc atagcgaagt tgatcgggac ttcgcaatac aggttgttgc
     3721 cttcacgctc gaaaatcggg tgctgtttaa cctgaacctg aacgtacaga tcgcctgccg
     3781 gtgcgccatg ctcgcccgct tcaccttcgc ccgcaagacg gatgcggtct ccagtgtcca
     3841 cccctgccgg gattttaacg gacagcgttt tgctgcgctc aacacgacca tgaccatgac
     3901 atttgttgca cggatctttg atcagcgtac cgcggccctg acagtgtgga caggtctgct
     3961 gtacagcgaa gaatccctgg cgcatctgca cctgaccaga accatgacag gtcggacagg
     4021 tctgcggctg tgtacctggt tttgcaccgc taccgtggca aacgtcacac tcttccagag
     4081 tcggaatgcg gatctctttg gtcacgccac gtacagcttc ttcgagggtg agctccatgt
     4141 tatagcgtaa atcagcaccg cgcgccgcac gttgacgacc acgtccgccg ccaaaaatat
     4201 cgccgaaaac gtcaccaaaa atatcgctga agtctgcgcc gccgccaaaa ccaccgccgc
     4261 ccatgcctcc ttgctcaaac gcagcatgac catactgatc gtatgccgca cgtttttgcg
     4321 agtcggtcag aacttcataa gcttccttga tctctttaaa tttcgcctcg gcctctttgt
     4381 caccctggtt acggtccggg tggtatttca tggccaggcg tttgtaggcc tttctgattt
     4441 cacgctcttc cgctgttttg gaaacgccta aaatctcgta ataatcttgc ttagccatct
     4501 tttttaaatt gcccctagat gaatgcacgg gcggagagga aattcccctt cgcccgtgtc
     4561 agtataatta cccgtttata gggcgattat tttttgtctt tgacttcttc aaattcagcg
     4621 tcgacaacat cgtcatcttt cgcgttgttt gcagaagcat cagcaccggc agtctgctgc
     4681 tgggcatgtt gctgctgggc gatttccatc agtttctggg aaacctgtgc cagttcctgc
     4741 attttcgctt cgatagcggc tttgtcttca cctttcagag cagtttccag tgcagtcagc
     4801 gcagactcga tagcagtttt gtcgtcagcc ggcagtttgt cgcctgcttc ttcaacctgc
     4861 ttacgggtgc tgtgcagcag atggtcgccc tggttgcgag tctgtaccag ctcttcaaac
     4921 ttacggtcag cttcggcgtt agcttctgcg tcgcgtacca ttttctggat ttcatcttcg
     4981 ttcagaccag aagaagcctt gatggtgatc ttctgctctt taccgctgtt tttatctttc
     5041 gcggaaacgt gcaggatacc gtcagcatcg atatcgaagg taacttcgat ctgcggcatg
     5101 ccgcgcggtg ccgggttgat accatctagg ttgaactgac ccagagattt gttatcagcc
     5161 gcacgtttac gttcaccctg cagcacatgg atggttaccg cagactggtt gtcttcagcg
     5221 gtagagaaca cctggctgtg cttggtcggg atagtggtgt ttttcgcgat cagcgtcgtc
     5281 atcacaccgc ccatggtttc gatacccaga gacagcgggg taacgtccag cagcagtacg
     5341 tcttttacgt caccagtcag aacaccaccc tgaacagcag caccgattgc tacagcttcg
     5401 tccgggttaa cgtctttacg cggctcttta ccaaagaact cagcaacttt cttctgaacc
     5461 attggcatac gagtctgacc accaacgagg ataacgtcgt cgatatcaga tacggacagg
     5521 ccagcatcct gcagtgcaac tttcagcggc tcaatggaac ggtttaccag atcttcaacc
     5581 aggctttcca gtttcgcacg agtcactttg atgttcatgt gtttcggacc ggtcgcgtct
     5641 gcagtgatgt atggcaggtt aacgtcggtc tgctgagcgg aagacagttc gattttcgct
     5701 ttttctgccg cttctttcag gcgctgcatt gccagcggat cgttgcgcag gtcaatgccc
     5761 tgatctttct tgaattcttc aaccagatag ttgatcagac ggctgtcgaa gtcttcacca
     5821 cccaggtggg tatcaccgtt ggttgccaga acttcgaagg ttttttcgcc gtcaacttcg
     5881 tcgatttcga taatagaaat atcgaaagta ccacccccca ggtcataaac cgcgatagta
     5941 cggttgccag tgcctttgtc cagaccgtaa gccagcgcag ctgcggtcgg ttcgttgatg
     6001 atacgtttta cttccagacc agcgatacgg cctgcgtctt tggttgcctg acgctgagca
     6061 tcgttaaagt atgccggtac ggtgataaca gcttcagtta ccggttcacc caggtaatct
     6121 tcagcggttt tcttcatttt tttcagcact tcagcagaaa tctgcggcgg tgccattttc
     6181 tggcctttaa cttcgaccca tgcgtcgccg ttatcagcag caataatttt gaacggcatg
     6241 atggaaacat cacgctgtac ttcttcgtcc tggaagcggc ggccaatcag gcgtttaatc
     6301 gcaaacaggg tgttttgcgg gttcgtcact gcctgacgtt tagccggctg accaaccaga
     6361 gtttcaccat cctgggtata ggcaatgata gaaggcgtgg tgcgatcgcc ttcggcgttc
     6421 tccagcacgc gaggaatggt gccatccata atcgctacac aagagttggt agtacccagg
     6481 tcgataccaa ttattttacc catctaaacg tctccactat atattcggtc atcatgtggt
     6541 tgtgagtctg taataggggc gaaacgtctg gtttcaactg cccaatttca tttttttgca
     6601 gactcactca ctgcggttga ctactaaatg gggtcgtaac ccacgtcatc aagggggaga
     6661 tgcaaaaatt ttttgtgctt ttgcgcagaa atttcgcgat atggcaggac aagacgtaaa
     6721 attgacagca attttattca tataaagaat gaatcgcagc caaggcgttc atcaaaaaat
     6781 tgtaataaaa agaaaagatt acgtgcctga atcttctctt tatcagcagt aaactagtgg
     6841 gtattcatcc ccctacctct tcccactaag agaatcctta tgaaatccgt ttttacgatt
     6901 tccgccagcc tggcgattag cctgatgctg tgctgcacgg cgcaggcaaa cgaccataaa
     6961 ctcctcggcg tcattgcaat gccgcgtaac gaaaccaacg atctggcgct gaaacttcct
     7021 gtttgtcgca ttgtgaaacg catacaactc tctgccgacc atggcgattt acagttaagt
     7081 ggtgcatcgg tttatttcaa agccgcccgt agcgccagtc agagcctgaa tattccttca
     7141 gaaataaaag aagggcaaac cactgactgg atcaacatta acagcgataa cgacaataaa
     7201 cgctgcgtct caaaaatcac cttttccggt catacggtga actcatcgga tatggccacg
     7261 ctgaaaatta tcggcgacga ctaacgccag aatatagaag ccacaaaaaa tgaatgttaa
     7321 ttacctgaat gattcagatc tggattttct tcagcattgt agtgaggaac agttggcaaa
     7381 tttcgcccga ttgctcaccc ataatgaaaa aggcaaaact cgcctctcca gcatactgat
     7441 gcgcaacgaa ctgtttaaat cgatggaagg gcatcccgag caacatcgcc gcaactggca
     7501 gctgattgcc ggagaattac agcattttgg tggcgatagt atcgccaaca aactgcgcgg
     7561 acacggtaaa ttgtatcggg ccattttgct cgatgtttca aagcgattga agctgaaagc
     7621 cgacaaagag atgtctacgt ttgaaattga gcaacagtta ctggaacaat ttctgcgtaa
     7681 tacctggaag aaaatggacg aggaacataa gcaggagttt ctgcacgcgg tcgatgccag
     7741 ggtgaatgag ctggaagagc tgctgccgct gctgatgaaa gacaaattat tggcaaaagg
     7801 tgtgtcgcat ttgctttcca gccaactgac ccgcatttta cgcacccacg cagcaatgag
     7861 cgtacttggg catggtttgc tgcgcggcgc ggggctggga ggccctgtgg gcgcagcatt
     7921 aaatggggtt aaagcggtca gcggcagcac ctatcgcgtg acgattccag ccgtactgca
     7981 aatcgcctgc ctgcgccgga tggttagcgc cactcaggtc tgataccgga aaccggggaa
     8041 aattttcatt ttcccctcaa aagatcgtag acactgcccc actggctgat tattatgccg
     8101 cgccctgaaa acactacagt tattcaggga aattatttca ccattcattc gatgatgatt
     8161 tttgaggaat tatgggcaac actaagttgg ctaatccggc accgctgggc ctgatgggct
     8221 tcggcatgac caccattctg cttaacctgc acaacgtggg ttatttcgct ctggacggta
     8281 ttattcttgc catgggcatt ttctacggcg gcatcgcgca aatttttgct ggtctgctgg
     8341 agtacaaaaa aggcaacact ttcggtttaa ccgcattcac ctcttacggt tctttctggc
     8401 tgacgctggt tgcgattctg ctgatgccga aactgggtct gaccgatgcg ccaaatgcac
     8461 agttccttgg tgtctacctg ggtctgtggg gcgtatttac gctgtttatg ttcttcggca
     8521 ctctgaaagg cgcgcgcgtc ctgcaattcg ttttctttag cctgaccgtg ctgtttgccc
     8581 tgctggcgat cggtaacatt gccggtaatg ccgcgatcat ccactttgcc ggctggattg
     8641 gtctgatctg cggtgccagc gcaatctatc tggcgatggg tgaagtgctg aacgagcagt
     8701 ttggtcgcac cgttctgccg attggtgaat cccactaatt gtcttgagat ccccccgctc
     8761 ggggggattt ttttattcgc taacgtcgcg tcttgcactc ttcggtctga atgcagcaac
     8821 cacttctggt gccgtttcaa cgtatggccc ttccagcaac tgaatgcagt acggtacgct
     8881 ggcaaaaata ccatgcacca caacgttacc ctcagcgtcc ttcacacctt ccagcgtctc
     8941 tttaatagac ttcggctgac cgggtaagtt aaggatcagc gcctgtttgc gaatcacgcc
     9001 cacctgacgc gaaaggatcg cagttggtac aaaatgcagg ctgatctggc gcatctgttc
     9061 accaaagcca ggcatctcgc ggtccgctac tgccagcgtc gcatcgggcg ttacgtcacg
     9121 acgcgccggg ccagttccgc ccgtggtgag caccagatgg caactcattt catccaccag
     9181 ctcacacaac gtttgctcga tgatcgcctg ctcatcgggg attaagcggg tttccagttc
     9241 aaacggcgtg gttagcgccg atgtcagcca ttcttccagc gctggaatgc ctttatcctg
     9301 ataaacgccg ctggatgcgc gatcagagat ggaaactaag ccaatacgta aagtattcat
     9361 caagattttt ccgtttaaac aatctcgtta aacagaatga tacactgcga agggagtgac
     9421 agacaggctt cagaagaggt agcgtgaccg acttcccggt cacgctaaga atgattacag
     9481 cagatcgccg atcatttttt ccagtttttc ctggtcaata gcaaacttac ggataccttc
     9541 cgccagttta tctactgcca ttggatcctg gttgtgctgc cacaggaact cggactcagt
     9601 gatacgcgcc ggacgcgctt tcacttcgcc ggtgtaagac agtttacgtt cgatagcccc
     9661 ttcgctctcc gccagctctt tcagcagtgc cggtgcgatg gtcagacggt cacagcctgc
     9721 cagttccaga atttcgccga tgttacggaa gcttgcgccc ataaccacgg tttcataacc
     9781 gtgctctttg tagtactggt agatttcaga tacagaaacc acgcccggat cttctgccgg
     9841 agcgtactct ttcttatcgg tattcgcttt gtaccagtca agaatacggc caacaaacgg
     9901 cgagatcagg aacacgcccg cctccgcaca agcacgagcc tgagcgaagg agaacagcag
     9961 ggtcaggtta cagttgatac cttctttttc cagctgttct gcagcacgga taccctgcca
    10021 ggtagaagcc agtttgatca gaatacgatc gttgctaata cctgcatcgt tgtagagttt
    10081 gatcaggcgt tttgctttcg caattgacgc ttcagtgtca taggaaagac gcgcatcaac
    10141 ttcagttgag atacggcccg gaaccagttt caggatttcc agaccaatat ttactgccag
    10201 tttgtcggtc gcgtccacga tctgctgcgc gcgatcgttg ctctgctgtt tcgcccaggc
    10261 gacagcatca tcaatcaact tacggtattc cggaatctgc gctgcgttaa gaatgagaga
    10321 agggttggtt gtggcatcct gcggttgata cagcttcatt gccgcgatgt ccccagtgtc
    10381 ggccactacg gtggtgtact gacgaaggga ggtcaatttg tccgtcatga tagtatttct
    10441 ctttaaacag cttgttaggg ggatgtaacc ggtctgccct gatgatatca cgacgcaacg
    10501 tcagcgcaac cgccgacaag acggttatcg ccaggcgaga ctgtttcgga tttctgacac
    10561 tgcgttgata tcgcccacca tttttataca aaacctcatg tatgctacgc agaagttatc
    10621 aagtacctcg tagcgtatat acttcttaaa caattggtaa cgtttacaca ggaaagtcat
    10681 cgcgaccggc aataagaggg atatgcatgc cagatttttt ctcctttatt aacagcgtcc
    10741 tttggggatc ggtaatgatt tacctgctct tcggcgcagg ttgttggttc acttttcgca
    10801 ccggatttgt gcagtttcgc tacatccgcc agtttggcaa aagtcttaaa aatagcattc
    10861 atccacagcc aggcggttta acctcatttc agtcattgtg taccagtctt gcggcgcgcg
    10921 tgggtagcgg caacctggcc ggcgttgcgc tggcaattac cgccggtgga ccaggtgccg
    10981 tcttttggat gtgggttgct gcgtttatcg gcatggcgac ctcgtttgcc gaatgttccc
    11041 ttgcacaact ttataaagaa cgtgacgtca atgggcagtt tcgtggcgga ccggcatggt
    11101 atatggcgcg cgggctgggg atgcgctgga tgggcgttct gttcgccgtc tttttgctca
    11161 tcgcctacgg cataattttc agcggagttc aggcgaacgc cgttgcccga gccctgagtt
    11221 tttcttttga ttttcctccg ctggtgacgg gcattattct cgctgtcttt gctctgctgg
    11281 caatcactcg tggtcttcat ggcgtcgccc ggctcatgca ggggtttgtc ccgttgatgg
    11341 cgataatctg ggtactgacc agcctggtga tttgcgtaat aaatatcggg caacttcccc
    11401 acgtcatttg gtctattttt gagagtgctt ttggctggca ggaagcggca ggcggcgcgg
    11461 cgggatatac cttaagccag gcgattacta acggttttca gcgcagtatg ttttccaatg
    11521 aggtgggaat gggatcgacg ccaaacgcgg cagcggcagc tgcatcctgg cctccgcatc
    11581 cggcagcaca agggattgtc cagatgattg gcatttttat cgacaccctg gtcatctgta
    11641 cggcaagcgc catgctgata ttactggcgg gtaacggcac aacctacatg ccgctggaag
    11701 gtattcagct tatccagaag gcgatgcggg tgctaatggg ttcctagggt gctgaatttg
    11761 ttaccctcgt ggttattctg tttgccttca gctccatcgt tgccaactac atttatgccg
    11821 aaaacaatct cttcttttta cgcctgaaca accctaaagc gatctggtgt ttgcggatct
    11881 gcaccttcgc aacggtcatc ggcggcacct tgctaagcct tccgctgatg tggcaactgg
    11941 cagatatcat aatggcctgc atggctatta ccaatttgac cgccatttta ctgctctcgc
    12001 ctgtggttca taccattgcc agtgattatc tacgccagcg taaactcggc gtgcgcccgg
    12061 tgtttgatcc gttgcgttat ccggatatcg gtcgccagct ttctccggac gcgtgggatg
    12121 atgtttcgca ggagtaatca caactatcga tcaactcatt ctcttttttt gctaaagtcg
    12181 gcataaattt cctgcaagga ctggatatgc tgattcttat ttcacctgcg aaaacgcttg
    12241 attaccaaag cccgttgacc accacgcgct atacgctgcc ggagctgtta gacaattccc
    12301 agcagttgat ccatgaagcg cggaaactga cgcctccgca gattagcacg ctgatgcgca
    12361 tcagcgacaa actggcgggt atcaacgccg ctcgctttca tgactggcag ccagatttca
    12421 cgccggcgaa tgcccgccag gcgattctgg cgtttaaagg tgatgtctac accggcttgc
    12481 aggccgaaac cttcagcgaa gacgatttcg attttgccca acagcatttg cgaatgcttt
    12541 ccggcttgta tggcgtactg cgcccgctcg atttaatgca gccttatcgt ctggaaatgg
    12601 ggatccgtct tgagaatgcc cgagggaaag atctgtatca attctgggga gatatcatca
    12661 ccaacaagct gaacgaggcg ctcgcagcac aaggcgataa tgtggtgatt aatctggcgt
    12721 cagatgagta ttttaaatcg gtgaagccga agaaattgaa tgccgagatt atcaagccgg
    12781 tgttcctcga tgagaagaac ggcaaattta agatcatcag cttctacgct aagaaagcac
    12841 gcggtctgat gagtcgtttc attattgaaa atcggctgac caaaccagag caactgactg
    12901 gttttaatag cgaaggttac ttctttgatg aagattcctc cagcaatggc gaactggtgt
    12961 ttaaacgcta cgagcagcgt taagtttgta gtcaatgccg gatgcggcgt gaacgcctta
    13021 tccggcctac aaaagcatgc agattcaata tattgcagaa ttaacgtagg cctgataaga
    13081 cgcgccagcg tcgcatcagg cgctgaatgc cggattgtta cccggcattt gtcatttagc
    13141 ggtgatgttt gcctggacca tgaccgccgt gatgatcatg aggagctttc ttatggtggc
    13201 gcggcggtgg cggcggtccg tgtgggtgcc agcgattgcc tcgccattca taatgttgtt
    13261 tccaccagcc gtggtcgcgc cagtgacctc catcccagta atagccacga ttatcacgat
    13321 cgcctatctg taattttact gacgggacta acgtaatttc cgcagcctgt gctgccatgg
    13381 gagcgaccag aaccagggaa agtgcgagta cgatagattg catctttttc acaggttact
    13441 ccttttatca ttgcccgtta tgggtcgata tccggagatt acgcgatgag aacgacggtt
    13501 gttattctcc gcaatcctaa tctctaagtt accgcattta ttgataattt ctcctttttc
    13561 cctgtcgttt ttctccataa tttcttcata aaaaagccgg gctgcataaa agcaaacccg
    13621 gcctgattga gataatgaat agattttact gatgattcat catcaattta cgcaacgcag
    13681 aaaaatcggc gggcagatta tgtgaaagca agggtaaatc agcacgttct gccagctctt
    13741 ttggcagatc caacgtttca ccgagaatcg cttccacgct ctctttaaat ttcgccggat
    13801 gcgcggtgcc gaggaacaag ccatattcgc ctggattcaa ctggtcacgc agcgcacgat
    13861 acgctacggc agcgtgcggc tccgaggtgt agcccagttc ttttaactca cgcattgtct
    13921 gttgcgtggt ttcatcatcc acggctgcat aacccagctc tttcagttgc cagattttgc
    13981 ggcggaacaa ctcttccaca cgcggccagt tgttcggctg actcacgtcc atcgcgttgg
    14041 ataacgtcgc ctgagtcgct ttgggtgacc actgaccgtc gtgcaggaaa cgtggcacgg
    14101 tatcgttcac gttggtcgca gcaataaaac gtttcaccgg cagaccgaat gacttcgcca
    14161 gcagacccgc cgtcaaatcg ccgaagtttc cgcttggcac cgagacaacc agctggttgc
    14221 gcgcctcctg cggcagctgc gcaacagctt caaagtagta gcaaatctgc gccagcaaac
    14281 ggctgatgtt aatcgagtta gccgagttta accccagcgc cactttcagt tcttcatcat
    14341 caaacgcctg cttcaccagc gcctgacagg catcgaaatc gccgtcgatg gcaacagttt
    14401 cgatattgcc gcccaacgta cagaacagtt tttcttgcag tggactgatt ttgcctcgtg
    14461 gatagaggat aaccactttc acattcggca aaccgtagaa agcatgagcc actgcagctc
    14521 cggtatcacc ggaggtcgcg gtcagaatgg tcactggctt atcatccgca atatgggtca
    14581 gcatttgtgc cataaagcga ccgccgaaat ctttaaatgc cagcgttggc ccgtggaaca
    14641 attccagaca accgacatcg ctttcaacat tggcgaccgg agccgggaag gcaaacgccg
    14701 cgcgcacgcg ctcttccagg atttcctgtg ggatttcatc accaataaac gccgagagga
    14761 tcttcgcact gcgggtgaca aaatccagct tcagcatctc atcaatttca gtcaggctga
    14821 attccggcag gtcgtgcgga aaaaacagcc cctgattttt gcccaacccc tgggttacgg
    14881 cttgcgcaaa gctgacctgc tcgttgtgat ctttcagatt gtagagtttc atttagtttt
    14941 ccagtactcg tgcgcccgcc gtatccagcc ggcaaatatg aacaaaacct tcctgatttt
    15001 gcaggtagtt cttacccaac cagtcggcaa cgcgctgggc ggtatccggc ttgtcacata
    15061 gagcgaacaa ggtcgggccg gagccggaga taccgctcgc taccgcgccg atttccgcga
    15121 ccgcctgccg cgcctgccgg aagccaggca gtaaccgttc acggtagggt tcagcgataa
    15181 catctttcat cagcttcgcg gcaagctcag gctgacggga atagcaggcg tgaatgaagc
    15241 cagccagatg tcgcccgtgc gcaatgcaat cctggcggcg atactgcgcc ggtaaaatag
    15301 cccgggcttc tgccgtcgag actttaattc ccggatacgc cagcacccac agccactcat
    15361 caaaccctgg cacttgctgg ctgatgatgt cgttttcttc gatcatcaac tgcataccac
    15421 caagaaaaca cggtgccacg ttgtcgtaat gaatgctgcc ggagatacgt ccttccagct
    15481 cgcccatcaa agccagcaaa cgagtgtcat taagcggctt gccgcagtgt tcattcatcg
    15541 ccatcagcgc cgcgaccacc gaacaggcgc tggagcctaa gcccgaaccg atcggcatat
    15601 tcttttccag agtcatcgcc actggaattt gcttgccaag ctcctggcaa aaacgctccc
    15661 agcactgata aacgatattt tcccgtggtt ctgacggcag cttatcggca aagcgtccga
    15721 gtttgttgag actgaatgtc tctgccgcct caaccgtgac tacatctccg agcaatgcac
    15781 catcaacggg tgccaccgcc gccccgagca catcaaaccc gacgctcata ttggcactgg
    15841 aagccggggc ataaacttta accatgtcag actcctaact tccatgagag ggtacgtagc
    15901 agatcggcaa agacaccggc agcggtaacg tcattgcccg caccatatcc gcgcagcacc
    15961 aacggcagcg gctgataata gtggctataa aaggccaggg cgttttcgcc atttttcact
    16021 ttgaacagcg gatcattccc atccacttcg gcaatcttca cgcggcagac accatcttca
    16081 tcaatattgc caacatagcg caaaactttt ccttcatcac gggccttcgc cacgcgcgcg
    16141 gcaaagagat cgtcgagctg tgacagattc gccataaaag cggcaacatc accctcggcg
    16201 ttaaactctg cgggcagcac aggttcaatt tcaatatccg ccagctccag ttcacgtccc
    16261 gtttcacgag cgagaatcaa tagtttacgc gccacatcca taccagaaag atcatctcgc
    16321 gggtccggtt cggtataacc catttcccgc gccagcgtgg tcgcctcgga gaaactcatg
    16381 ccttcgtcta acttgccgaa gatataagaa agcgaacctg aaagaatgcc ggagaacttc
    16441 accaattcat cacctgcatt gagcagattt tgcaggttct caataaccgg taatccagcc
    16501 ccaacgttgg tgtcatagag gaatttacgc cgcgattttt ccgccgcata acgcaactga
    16561 tgatagtaat ccatcgacga ggtgttggcc tttttgttcg gcgtgacaac gtggaaacct
    16621 tcgcgcagga agtcggcata ttgatccgcc actgcctggc tggaggtgca gtcaacaatg
    16681 accgggttca gcagatgata ttctttcacg aggcgaatta agcgcccgag attaaacggc
    16741 tctttggctt gtgcaagttc ttcctgccag ttttccagat taaggccatg cacactagtg
    16801 agcagagcct tcgagttggc aacgccgcag acacgtaagt cgatatgttt attcttcagc
    16861 cagctttgct gacgcttcag ttgctccagc agcgcaccgc caacgccacc gacgccaatc
    16921 acaaacactt cgataacctg atcggtattg aacagcatct gatgagtaac gcgcacgcca
    16981 gtggtcgcat catcgttatt taccacgaca gagattgagc gttcagaaga tccctgagca
    17041 atggcgacaa tgttgatatt ggcgcgggcc agcgcggcaa agaatttcgc cgagatccca
    17101 cgcaaggtgc gcataccatc acctaccacc gagataatgg ccagccgttc cgtcaccgcc
    17161 agcggctcca gtaagccttc tttcagttcc aggtagaact cttcctgcat tgcccgttca
    17221 gctcgcacac agtcgctttg tggaacgcag aaactgatac tgtattcgga agatgattgc
    17281 gtaatcagca ccacggaaat acgggcgcgt gacattgcag caaagacgcg cgccgccatg
    17341 ccaaccatcc ctttcatccc cgggccggaa acgctgaaca ttgccatgtt attcagattg
    17401 gaaatgccct tgaccggtaa ttcgtcttca tcacggctgg caccaatgag cgtacctggt
    17461 gcttgaggat ttccggtatt tttaatcagg caagggatct ggaactgggc gatgggggta
    17521 atggtgcggg ggtgaagaac tttagcgccg aagtaggaaa gctccatcgc ttcctggtag
    17581 gacatcgact tcaacaacct cgcatcgggc acctgacgcg ggtcgcaggt atagaccccg
    17641 tcaacgtccg tccaaatctc gcaacaatcg gcgcgtaaac aggcagccag caccgccgcg
    17701 gagtagtcgg aaccgttacg tccaagcacc accagttcgc ctttttcatt accggcggtg
    17761 aaacctgcca tcaacaccat gtgatcagcc ggaatgcggc ttgccgcaat acggcgggtg
    17821 gactcagcaa tatcgacggt agattcgagg taatgcccca ctgccagcag tttttcgacc
    17881 ggatcgataa cggtaacgtt gtgaccacgc gcttctaaca cgccggccat aatggcgatc
    17941 gacattttct cgccacggca aatcagcgca gcgttgatgc tatccgggca ctgccccaac
    18001 aaactaatgc catgcaggac atgttttatt tgggcaaatt cctgatcgac gaaagttttc
    18061 aattgcgcca gcgggaaccc cggctgggcg gcggcgagtc ccgtcaaaag ttcggcaaaa
    18121 atacgttcgg catcgctgat attgggtaaa gcatcctggc cgctaatggt tttttcaatc
    18181 atcgccacca ggtggttggt gattttggcg ggggcagaga ggacggtggc cacctgcccc
    18241 tgcctggcat tgctttccag aatatcggca acacgcagaa aacgttctgc atttgccact
    18301 gatgtaccgc cgaacttcaa cactcgcatg gttgttacct cgttaccttt ggtcgaaaaa
    18361 aaagcccgca ctgtcaggtg cgggcttttt tctgtgtttc ctgtacgcgt cagcccgcac
    18421 cgttacctgt ggtaatggtg atggtggtgg taatggtggt gctaatgcgt ttcatggatg
    18481 ttgtgtactc tgtaattttt atctgtttgt gcgctatgcc tatattggtt aaagtattta
    18541 gtgacctaag tcaataaaat tttaatttac tcacggcagg taaccagttc agaagctgct
    18601 atcagacact ctttttttaa tccacacaga gacatattgc ccgttgcagt cagaatgaaa
    18661 agctgaaaaa tacttactaa ggcgtttttt atttggtgat atttttttca atatcatgca
    18721 gcaaacggtg caacattgcc gtgtctcgtt gctctaaaag ccccaggcgt tgctgtaacc
    18781 agtcgaccag ttttatgtca tctgccactg ccagagtcgt cagcaatgcc atgactcgtt
    18841 cgcgtaaagc ttgcagttga tgttggtctg ccgttgtgtc acttttcgcc ggttgttgta
    18901 ttaatgttgc taattgatag caatagacca tcaccgcctg ccccagattg agcgaaggat
    18961 aatccgccac catcggcaca ccagtaagca cgtcagccaa cgctaactct tcgttagtca
    19021 gcccggaatc ttcgcgacca aacaccagcg cggcatggct catccatgaa gatttttcct
    19081 ctaacagcgg caccagttct actggcgtgg cgtagtaatg atatttcgcc cgactacgcg
    19141 cagtggtggc gacagtgaaa tcgacatcgt gtaacgattc agccagtgtc gggaaaactt
    19201 taatattatc aataatatca ccagatccat gtgcgaccca gcgggtggct ggctccaggt
    19261 gtgcctgact atcgacaatc cgcagttcgc taaaacccat cgttttcatt gcccgcgccg
    19321 ctgccccaat attttctgct ctggcgggtg cgaccaggat aatcgttata cgcatattgc
    19381 cactcttctt gaccaaataa ccgcgaacag ggtgatcact gtcaacttat tacgcggtgc
    19441 gaatttacaa attcttaacg taagccgcag aaaaagccct ttacttagct taaaaaaggc
    19501 taaactattt tctgactgca ctaacggttt aattgttaaa aaatgctaca tatccttctg
    19561 tttacttagg ataattttat aaaaaataaa tctcgacaat tggattcacc acgtttatta
    19621 gttgtatgat gcaactagtt ggattattaa aataatgtga cgaaagctag catttagata
    19681 cgatgatttc atcaaactgt taacgtgcta caattgaact tgatatatgt caacgaagcg
    19741 tagttttatt gggtgtccgg cccctcttag cctgttatgt tgctgttaaa atggttagga
    19801 tgacagccgt ttttgacact gtcgggtcct gagggaaagt acccacgacc aagctaatga
    19861 tgttgttgac gttgatggaa agtgcatcaa gaacgcaatt acgtacttta gtcatgttac
    19921 gccgatcatg ttaatttgca gcatgcatca ggcaggtcag ggacttttgt acttcctgtt
    19981 tcgatttagt tggcaattta ggtagcaaac atgcagaccc cgcacattct tatcgttgaa
    20041 gacgagttgg taacacgcaa cacgttgaaa agtattttcg aagcggaagg ctatgatgtt
    20101 ttcgaagcga cagatggcgc ggaaatgcat cagatcctct ctgaatatga catcaacctg
    20161 gtgatcatgg atatcaatct gccgggtaag aacggtcttc tgttagcgcg tgaactgcgc
    20221 gagcaggcga atgttgcgtt gatgttcctg actggccgtg acaacgaagt cgataaaatt
    20281 ctcggcctcg aaatcggtgc agatgactac atcaccaaac cgttcaaccc gcgtgaactg
    20341 acgattcgtg cacgcaacct gctgtcccgt accatgaatc tgggtactgt cagcgaagaa
    20401 cgtcgtagcg ttgaaagcta caagttcaat ggttgggaac tggacatcaa cagccgttcg
    20461 ttgatcggcc ctgatggcga gcagtacaag ctgccgcgca gcgagttccg cgccatgctt
    20521 cacttctgtg aaaacccagg caaaattcag tctcgtgctg aactgctgaa gaaaatgacc
    20581 ggccgtgagc tgaaaccgca cgaccgtact gtagacgtga cgatccgccg tattcgtaaa
    20641 catttcgaat ctacgccgga tacgccggaa atcatcgcca ccattcacgg tgaaggttat
    20701 cgcttctgcg gtgatctgga agattaatcg gctttaccac cgtcaaaaaa acggcgcttt
    20761 ttagcgccgt ttttattttt caaccttatt tccagatacg taattcatcg tccgttgtaa
    20821 cttctttact ggctttcatt ttcggcagtg aaaacgcata ccagtctata ttacgagtca
    20881 caaacatcat gccggccagc gccaccacca gcacactggt tcccaacaac agcgcgctat
    20941 cggcagagtt gagcagtccc cacatcactc catccagcaa caacagcgcg agggtaaaca
    21001 acatgctatt gcgccagcct ttcaataccg cctgcaaata aataccgttc attaacgccc
    21061 caatcagact ggcgattatc catgccacgg taaaaccgat atgttcagaa agcgccagca
    21121 agagcaaata aaacatcacc aatgaaagcc ccaccagcaa atattgcatt gggtgtaaac
    21181 gttgcgcggt gagcgtttca aaaacaaaga acgccataaa agtcagtgca atcagcagaa
    21241 tggcgtactt agtcgcccgg tccgttaatt ggtattgatc ggctggcgtc gtcactgcga
    21301 cgctaaacgc ggggaagttt tcccagccgg tatcattgcc tgaagcaaaa cgctcaccga
    21361 gattattagc aaaccagctg ctttgccatt gcgcctgaaa acctgactcg ctagcttccc
    21421 gtttggctgg tagaaaatca cctaaaaaac tgggatgcgg ccagttgctg gttaaggtca
    21481 tttcgctatt acgcccggca ggcaccacag aaagatcgcc ggtaccgctt aaattcagtg
    21541 ccatattcag ctgcaggttc tgcttccgcc agtccccttc gggtaaaggg atatgcacgc
    21601 cctgcccgcc ttgctctaac ccggtaccgg gttcaatggt cagtgccgtt ccgttaactt
    21661 caggcgcttt caccacacca ataccacgcg catccccgac gctaatcaca ataaagggct
    21721 tgcctaagat gatatttggc gcgttgagct cgctaagacg cgaaacatcg aaatcggctt
    21781 ttagcgttaa atcactgtgc cagacctgac cggtataaat ccctatcttg cgttcttcca
    21841 cgttctgatt gccatcaacc atcaatgact caggtaacca aaaatggata aaacttcgtt
    21901 tccgctccac ggttttatcc tcttcctgca ccgtataaag ctcggtcaca ggaatagcga
    21961 taagcggccc aacgagtttt tgcggcccgc tggtactttg gcgaatcgca tcttccacat
    22021 cgctacggta atcggcacgt tcgacaatca cttgccgaat cagcattatc ggaatcaaca
    22081 acagcaatac tgcaccaaac aaggtggtca ttttccagaa caggggggat ttcaacatag
    22141 tcttctcctt tgcaatggca gcaggatacg aaggctatgt gggaagaatt tgaagctatg
    22201 tgaagtgacg gtgaagtcgt agcgaggcca gcacgccacc ttcctgcacg ttgcgcagcg
    22261 tgacttcgcc gttaaacaaa cgggcgacct cactgacgaa cgccaacccc agaccgctgc
    22321 ttttttgccc atttgcacgc ggcaaagagt aaaagcgttc aaaaatacgt gacagcgcgt
    22381 agtcaggaat accactaccg gtatccagca ccttaagcgt gacgtgttcc tgatccactt
    22441 cggcgcttag cgttatgcaa ccgctctcgg gagtaaaatc gatggcgtta tccagtaaat
    22501 tccccagcgc ctgatccagt aacgccggtt cagcagcaac attaacctcg gtgggcataa
    22561 catgcaaagt gatgtttttt tctgccaact gcacggtgcg cgcttcgctg acgcggcgaa
    22621 ataatgccgc cacatcaaca gcagtcagaa cgacttcctg acgattctcc agtcttgcct
    22681 ggcgtagtaa cgtttccacc agcgcctgca ttcgcgcatt ttgtgtcaga atgttgtcag
    22741 taaaacgagc cactacttca ggcggcggac cttcgcgtaa aatttccgcc gcgccacgaa
    22801 tcgccgccag tgggcttttt agctcatggg ttaacgcata aacatactgc tcaatatagt
    22861 ttttcccttc cagcttcacg cgcatacttt ccagcgcctg cgcgagttta cgcagctcgc
    22921 tactaccgag atcgggaaga ggaacgggct tattgtcagt gacggaatca gcatagcgag
    22981 tgagccgggc aatggagcgg ttgatccacc aaaccatgcc tgcgccaatc accagtgcaa
    23041 tccccaacaa aatggcgctg gcccataaaa ttcgccgctc gctgcgttta atgaccggag
    23101 ccatcgccgc gttcggtttg cctacgctca aaacgccaat aagccgcgag ccgcccataa
    23161 tcggcgcggc aacatacatc accgaacttt cgggatcggc aggattttgc aacgtgctgc
    23221 gcgcaccata ctgaccacgc aacgttagcc agacgtcatt ccagcgcgaa taatcctgtc
    23281 caacggcttt atttgccgaa tcgaacaata ctttgccatg cgcatcggtc atatagacat
    23341 gatattcatt gcgcactttg ttaatgccac caatattggc gcgaaacggg cgatgttgta
    23401 gctgattaaa cgcctgcgcc agttgcccat gcgttgggtc cccagagagc aaatcgggac
    23461 gcgccagctc cgcaagtaac gttgccgtgt cgatcaacgt cccctccgtt gctcttcgca
    23521 cgcccggttt aacttctttg acaaaaatag ccagtacgaa ccaggccgcc accgccacca
    23581 gtaaaaaata gcccagcagc aaccgcatgc cgatacgcat tacaagcccc tcaggctata
    23641 tcccatgccg cgatgagtat taatcggtga aagatcgggg ttgatggcgc gcagcttggc
    23701 acgcagcgtt ttaatgtgga tatcgacggt gcgatcgtag gtgtcctgcg catcttccca
    23761 cacgctatcc attagttgct ggcgggacca gacgcggcct ggcgacttga gtaacgtctt
    23821 cagcaataaa aactcatacc gcgtcagcgt taatggcgtg tcaaaccagc tgatctgcgc
    23881 cgcgggttca ttcaattcaa aatgaccaat acggatgacg ggagacggcg tcgagaactt
    23941 cttcacccga cgcagtaagg tgcgcaccct ggcgcacact tcgcggggtg aaaacggttt
    24001 agccacatag tcgtcagcgc caatttccag cccaagcagg cgatcgacct cttcacttcg
    24061 ggccgtcagg aacagtacag gtaacgccgg atagagcgcc agtaactggc ggcacaattc
    24121 aaagccactg atatcaggca aaccaacgtc gagaatcatg acgtcgggaa cctgctggcg
    24181 agctttatcc agcaccggca aacctcgctc aaagacctcg acggcaaaac cttcctgctg
    24241 caacatgtag accagcgtgt cggctatccc ttgctcatct tccactaacc agaccgtttc
    24301 ccgttgcata aatcgcctct gttattgccg ccacggcatg acaggaaccg cgctaatcgc
    24361 gtttttcggc gaaccttcta caactttgtc ggagtaagcc agataagcga gcgcgttgcg
    24421 tttggcgtca taaaagcgca cgacctgtaa cgacttaaag accagggacg tgcgtttttt
    24481 gaatactacc tcgccctgag ctttgccgtt tttaatacga tccgacagtt caatcggccc
    24541 gacttgctga caagaaatgg ccgcatcgga ggtatcttct gccaggccca atcccccttt
    24601 aataccaccg gttttcgccc ggctcacata acaggtgaca tttttcacat cgggatcgtc
    24661 aaaggcttcc acaacaattt tgtgatccgg gccgatcatt ttaaatacgg tgtcgaccga
    24721 accaatctct tctgcatgag ccaatggccc cagcattatc aggcttaaag acaggatcaa
    24781 atgcttgtat ttcatattgt taccattttt atctattaca cgagcaatta gttcgtcacg
    24841 gtaaataaca tattgattaa gaacaaaatc tcaattcttt tatttccgtc aagccctaaa
    24901 acatactcta ctaagaaaaa aacactgaat gctaaaacag caaaaaatgc tattatccaa
    24961 ttacctgatg tcaggtgctc gttgttgaaa ggatgaggat attttatgga tcaggccggc
    25021 attattcgcg accttttaat ctggctggaa ggtcatctgg atcagcccct gtcgctcgac
    25081 aatgtagcgg cgaaagcagg ttattccaag tggcacttac agagaatgtt taaagatgtc
    25141 actggccatg ctattggcgc gtatattcgt gctcgtcgtt tgtcgaaatc ggcggtcgca
    25201 ctacgcctga ctgcgcgtcc gattctggac atcgcgctgc aataccgctt cgactctcaa
    25261 cagacattta cccgcgcatt caagaagcag tttgcccaga ctcctgcact ttaccgccgt
    25321 tctcctgaat ggagcgcctt tggtattcgc ccgccgctac gtctgggtga attcacaatg
    25381 ccagagcaca aatttgtcac cctggaagat acgccgctga ttggtgttac ccagagctac
    25441 tcctgttcgc tggagcaaat ctctgatttc cgccatgaaa tgcgttatca gttctggcac
    25501 gattttctcg gcaacgcgcc gaccattccg ccggtgctct acggcctgaa tgaaacgcgt
    25561 ccgagtcagg ataaagacga cgaacaagag gtattctata ccaccgcgtt agcccaggat
    25621 caggcagatg gctatgtact gacggggcat ccggtgatgc tgcagggcgg cgaatatgtg
    25681 atgtttacct atgaaggtct gggaaccggc gtgcaggagt ttatcctgac ggtatacgga
    25741 acgtgcatgc caatgctcaa tctgacgcgc cgtaaaggtc aggatattga gcgatactac
    25801 ccggcagaag acgccaaagc gggagatcgc ccaattaatc tgcgctgtga actgctgatt
    25861 cctatccgcc gttaacgctg cagctcatct aatgcagggg cgtccagatg cgagatgtcc
    25921 cctgcggttt caacgaccca gccagacgcc agccacaggc tttcctgata atccacgcgc
    25981 gaaatagaac agttacgcag acgtaagcgg cgctctgccc atgctggtaa tccgagaatc
    26041 gtactcacca gacatcccag tgcaatacca tggctcacca gcagcggtcg gcttccctgc
    26101 ggtaagtccc ggcaagattc cagtgccgca ttgacgcgat cgctgagctc ctgcattgac
    26161 tcgccttcag gaatacgccc gtcaacggtg ccattgacca gctgccgacg ccagttctct
    26221 tcttcttcgg tcagagaatc gatatgtctt ttttccagca cacccatgtt taattcacgc
    26281 aggcgagaat caaagatgat gtcacagccg caggcctggg cgatgatttc cgccgtacgc
    26341 cgggtgcgtc ctaaatcgct actgataata tgcgtaatgc caagctcttt ggcacgggtt
    26401 gccacctgca tcgcctgttg ctcacctttg gcggtcagcg ggctgtcaga ctggccctga
    26461 atacgtcgct cggcgttcca ctgcgtttca ccgtggcgga ctaggtatac ctgtaacatg
    26521 ctttttttcc gtatactgct gtaacgttaa ttttctgagt aatgctgatt atgcaccaag
    26581 ttgtctgtgc gaccaccaat cccgctaaaa ttcaggccat tctgcaggca tttcacgaga
    26641 tcttcggcga aggatcctgc catattgcat ccgttgccgt cgagagcggt gtaccggaac
    26701 aaccctttgg cagtgaggaa acgcgcgctg gcgcacgaaa tcgggtagca aatgcccgcc
    26761 gtttacttcc agaggctgat ttttgggtgg cgattgaagc tggcattgat ggcgacagca
    26821 ctttcagttg ggtggtgatt gaaaacgcca gccagcgcgg cgaggcgcgt tcggcaactt
    26881 tgccgttacc tgcggtgatt ctggaaaaag ttcgtgaagg cgaggcgctt ggtcccgtga
    26941 gtcgcgttat accggtattg atgagattgg tcgtaaggaa ggggcgatcg gcgtgtttac
    27001 cgccgggaaa ctcactcgcg ccagcgtcta tcaccaggcg gtgatcctgg cgctgagtcc
    27061 gtttcacaat gccgtgtatc aggcattgta ggacggataa ggcgttcacg ccgcatccga
    27121 cattcggtgc acgatgcctg atgcgacgct gccgcgtctt atcaggccta caaaatcaat
    27181 cgcttttcag caacacctct tccagccact gacgcagctc cacgggcgcg gctttcaggc
    27241 tgttagatcc acgcgtaatc gtcgcgatgc ccgcgccgag ttcatttttt aactcacgct
    27301 gactcatttc gccacgcaac agctcttcga caatacgcac gcgagtcccc aacgcttcgc
    27361 gctcatctgg cgtcagcatc aggtttaaca acggtaaatg gagatcgttt tggtaggcat
    27421 tcttaagcag gtcgacaaaa cgtaaccact cctggtgacg ctgttctgcc atcgctgctg
    27481 aatagggtga ttgttgggcc ataatatgtc gccattgtta gcgggggaag caaaatgact
    27541 cccccggttg tactcgctaa agagtacgat agcatatcat aaacgtgcgg atcagtaacg
    27601 acgtccccat tccgtggcgc tcatcaacgt cggtttatcc cccatgaaat agcggtagta
    27661 agcatcataa gccagcacgt tcttcacata accgcgcgtc tcggagaacg gaatactctc
    27721 gacaaatgcc acagcgtcga tacgcccggc gctgttgcca agccaggttc gcacccgtcc
    27781 aggtccggcg ttataagctg ctgaggagaa aatacggtta ttgccaaact gctgataaac
    27841 atattgcagg taactggtgc caatgttgat gttcgtttcc ggatccagca attgcccagg
    27901 gctgctataa ccaggaatag agaacatctt caccgtatgg gtcgctgtac caggcataat
    27961 ctgcatcagg ccgctggccc ctaccggtga tttcactttc ggattccagg cgctctcctg
    28021 acgggcaatc gccatcgcat agctttgcgg gatctctttc ccactggtgt aacgtttgaa
    28081 aagatcgttg taagccagcg ggaatcgctc ttccagatga tcccaaagct tcccggcgat
    28141 cgttgcctga acgctaagat cccaccattg gttattgaaa gcataccgag ccagttgagc
    28201 ctgctctgtt tttgacttgc tcttcaccag attggcccac tcgctacgcg cggtattatc
    28261 gagattccag tacatcaact cgcgcacgcg cgccatctcc ggcccctgag tcagggtgct
    28321 gtcaacattc tgcggcgctt tatcaatctt cagctcatac tcttcaccga tgcgttgtgc
    28381 tgcaaccatc gggtagaaac cacgctgttg tatgagttga tgcaaaatct ctttcgcttc
    28441 agcttcacgt ccgcgttcca gcaataaatc cgcctgccag taacgccatt catctttctc
    28501 ttttgcttcc atcggcagac gcgccagcca ggtattcagg ccgcgacgat cgccggtgcc
    28561 aagcgccatt cgcacacggc gttcaatcaa cgacgtggat tgcgagcgca taatggcgtc
    28621 atcgcgccat ttcgcctgct catcggtgac atcattgccc atcaaacgcc aggcgacgat
    28681 atcgcgcagc tcctgaatct gatcttcatt aagctgctgc gcctgggcaa gcgacgggat
    28741 catcagccgt gcattttcgg catcctgccg cgccacactg gcaaacgcca ccgccgccat
    28801 ttgacgggta aaatcggtcg cgccggttgt acgcgcgaag gtcagtaccg tattagggtt
    28861 gttcgccagt gaaatgattg ccgaggcgat agtctggtaa tcggcaggca tctgccctgc
    28921 cagcactgtt accaggcctg tgttacccgc tttcatcgcc agacggatac gctctaaata
    28981 cgccagcgga tcttgtttac ctgacgcacg ccagacgcta aataacttgt cacaggcgtt
    29041 aggctggctc ttgccggtta gccacagctc tttcgcccct tgccaggctt cttcactctg
    29101 cccggtgttc catttcgcat agtagtaatt acattgcgct tcggtagttc cgggcttttc
    29161 cgggctaaag gctaacaagc cacgccagtc ttcacgccgc gccagttcat tgacgaaacg
    29221 agattgcagc gtgcgagcgg gaggaagcgt ggggttagcg cgaacaaagt tagtgaccgt
    29281 caccgccggt tgattcatca ggtcatcggt gatctggcgg tattccaggt agggataaag
    29341 cggataatcc ttcagtccag gcatcatttg ttcgaccaca tccatctgtc gattatccca
    29401 ggcctgcttg atttgcgcgt aacggctacg ctgctcatcc agtgagtcgg ctcgcgccac
    29461 gctgctgacc gtcagcagac agacaccggc agccaacagc cgccaggtaa cttgtttggc
    29521 tttttccaca agcgcatcct ctaagtgtaa atacatcaat gcagcgtcat gccgcgtaat
    29581 agatttatgc taaccagtca ttgccgttta cgccacgtta cggacacttt tttactttta
    29641 ctgcgaaggt gatcgaccaa ctgggattag gcggggaaac aggctacact tcgcctttga
    29701 aaactcatca tcacgataaa catagaggcg aagtccaacg tggctcaatt cgtttatacc
    29761 atgcatcgtg tcggcaaagt tgttccgccg aaacgtcata ttttgaaaaa catctctctg
    29821 agtttcttcc ctggggcaaa aattggtgtc ctgggtctga acggcgcggg taagtccacc
    29881 ctgctgcgca ttatggcggg cattgataaa gacatcgaag gtgaagcgcg tccgcagcca
    29941 gacatcaaga ttggttatct gccgcaggaa ccgcagctga acccggaaca caccgtgcgt
    30001 gagtccattg aagaagcggt ttcagaagtg gttaacgccc tgaaacgcct ggatgaagtg
    30061 tatgcgctgt acgccgatcc ggatgccgat tttgacaagc tggccgctga acaaggccgt
    30121 ctggaagaga tcattcaggc tcacgacggt cataacctga acgtacagct ggagcgtgcg
    30181 gcggatgcgc tgcgtctgcc agattgggac gcgaaaatcg ctaacctctc cggtggtgag
    30241 cgtcgtcgcg tggcgttgtg ccgcctgctg ctggaaaaac cagacatgct gctgctcgac
    30301 gaaccgacca accacctgga tgccgaatcc gtggcgtggc tggaacgctt cctgcacgac
    30361 ttcgaaggca ccgtggtggc gattactcac gaccgttact tcctcgataa cgttgcaggc
    30421 tggatcctcg aactggaccg cggtgaagga attccgtggg aaggtaacta ctcctcctgg
    30481 ctggagcaga aagatcagcg tctggcgcag gaagcttcac aagaagcggc gcgtcgtaag
    30541 tcgatcgaga aagagctgga gtgggtacgt cagggaacta aaggccgcca gtcgaaaggt
    30601 aaagcacgtc tggcacgctt tgaagagctg aacagcaccg aataccagaa acgtaacgaa
    30661 accaacgaac tgtttattcc acctggaccg cgtctgggcg ataaagtgct ggaagtcagc
    30721 aacctgcgta aatcctacgg cgatcgcctg ctgattgatg acctgagctt ctcgatcccg
    30781 aaaggggcga tcgtcgggat catcggtccg aacggcgcgg gtaaatcgac cctgttccgt
    30841 atgatctccg gtcaggaacc gccggacagc ggcaccatca ctttgggtga aacggtgaaa
    30901 ctggcatcgg ttgatcagtt ccgtgactca atggataaca gcaaaaccgt ttgggaagaa
    30961 gtttccggcg ggctggatat tatgaagatc ggcaacaccg agatgccaag ccgcgcctac
    31021 gttggccgct ttaactttaa aggggttgat cagggtaaac gtgttggcga actttccggt
    31081 ggtgagcgcg gtcgtctgca tctggcgaag ctgctgcagg ttggcggcaa catgctgctg
    31141 ctcgacgaac caaccaacga cctggatatc gaaaccctgc gcgcgctgga aaacgccctg
    31201 ctggagttcc cgggctgtgc gatggttatc tcgcacgacc gttggttcct cgaccgtatc
    31261 gccacgcaca tcctggacta ccaggatgaa ggtaaagttg agttcttcga aggtaacttt
    31321 actgagtacg aagagtacaa aaaacgcacg ctgggcgcag acgcgctgga gccgaagcgt
    31381 atcaagtaca agcgtattgc gaagtaatgc gtaaaatgcc ggatgcggcg cgaacgcctt
    31441 atccggccta caaaaccagc aatttcaata cgttgtaaaa ctgtaggcct gataagacgc
    31501 ggtaagcgtc gcatcaggca ttgagcaccg aatgccggat gcggcgtgaa caccttttcc
    31561 ggtctacaac agccttagca tcctcaattt cgtggcgggt cacccgtgca ggttttttca
    31621 aaccttgttt aatctcgaca gcctcttcca gagaggtttt caactcatca aaaaaactca
    31681 ttttatacct cgcctttgag cagtttcgtc acggttatcc ttgctccccc atcatctccc
    31741 gcaccagctc cacgcagcgc aggaaacggc tgtcgtaatc gtcctcttca acccgcacga
    31801 attcgatatt gttctcttcc agcatctcca ccagcaagtt ctggaactct ttgcgatcca
    31861 ccgaactgcc gaggctgcgt aaaccatccg ccacccacgg cgtgttgttc tccagcagga
    31921 tcaccagatc gaaacggtat tcatcaatca gcgcctgcac gaacggatgc tcacgccctt
    31981 cgtacttttt gcagaacgcc tgggtagtga caaaatcggt gtcaataaac gccactttat
    32041 tggcgtattt cactgcaaaa tcaatgtatt gtgcgtgacc caacgcgatt ttatcgtagt
    32101 cggaatactg caatgcgatt tcatcaccgc cgaggtgtga aaagacataa tcgcggccat
    32161 attcccacgc actggtggtg ttgaagatat tggcaagttt gtttaccagg gtggatttac
    32221 cgctcgactc gccgccaagg atcgccacgg tacgcacaaa aaacggcttc acttcggtag
    32281 gaatatattc ccagtagcgg aacgggtttt cgcggatctg cgcaccgctg atactcataa
    32341 aggtacgttt cggatcgacc agcaccgtct cgatccccag atgttccata tactgtggcg
    32401 catcggcttc ttccgaggtg tagatcaggt ccggctggat ccccttttca gccataaact
    32461 ttttgatgcc gttgctccac acatcccagc catgcggata cggctccatg ccctcttcgt
    32521 tgaaagcatg aatacggata tttttctgat atttaaaggt ttgcaatagc cagcgcagac
    32581 gatccggcac cgtgggctgc tgcgacatgg cgctgtcttc aaacaacgcg cggtcgcggg
    32641 tatcgtcaaa acccataatg atatgcagct cgtcaacctg gctacaggcg cgctggataa
    32701 ggtagatatg tccggtatgc agcgggtaga acttaccgaa tacgacacca atggttttct
    32761 tctgccgggg aaattcaagc cccaaaaaac ggtgcaatgc ctccagcttt tgcgcgctgg
    32821 ggcttttaat tttggcattc agtaactggc ttaaataccc tttggtcata ccgctggcat
    32881 cagctacctg ctgtagcgtg cagccctgtt gcttgatggc agttttcagg taatcaaatg
    32941 acgacatatc tccctccgta tctctcatta taagtcgtcg aacacgctaa gcgcgtcgga
    33001 gagtttttta acgccaaaaa tctgcatccc ttccggcgct tttttcggta cgttagccgc
    33061 cggaacaatc gcccggcgaa aaccgtgttt cgccgcttca gaaatccgct cctggccact
    33121 gggcaccggg cggatctccc ctgccagccc gacttcacca aacaccacca gatcctgtgg
    33181 cagcggtctg tcacgcaggc tggaaaccat cgccagcagt aacgctaagt cggcgctggt
    33241 ttcggttacc ttcacgccgc cgaccacgtt cacaaacaca tcctgatcgg ccatttgcag
    33301 accaccgtga cggtgcaaga cagccagcag gattgccaga cggttttgtt ccagccccac
    33361 tgccacgcgg cgcggattcg ccattatcga gtgatcgacc agcgcctgaa tctccaccag
    33421 caacggacgc gttccttccc acaccaccat cacggagctg ccggaggtca cttcatctcc
    33481 gcgacttaag aaaattgccg aagggttgct gacttcacgc agcccctgtt cggtcatcgc
    33541 gaagacgccc agctcattca ccgcgccgaa gcggttttta tggctgcgta aggtgcgaaa
    33601 acgggagtcg gcatcgccat ccagaagcac cgaacagtcg atacagtgtt ccagcacttt
    33661 cgggccagcc agcgaaccat ctttggttac gtgtccaacc atgacaatcg ccacaccgcg
    33721 cgttttggcg aaacgcgtca gataagccgc cgtttcacgc acctgcgcca cgctaccagg
    33781 cgatgattgt acatccgcca tatgcatcac ctggatcgag tcaattacca tcagcttcgg
    33841 ttgctcttct tcggcaatca ggcagatctg ctcgatgctg gtttccgaca acatattgag
    33901 attgtcagtc ggcaggccaa ggcgatgagc gcgcattgcc acctgttgca gcgactcttc
    33961 gccggtgaca tacagcgttt tcatctgctg ggccagtttg cacagcgttt gcagcaacag
    34021 cgtggatttc cccgcaccag ggttaccgcc aatcagaatg gcacttcctg gcaccacgcc
    34081 gcctcctagt acgcggtcga actctttaaa tccggtggaa aaacgcggca gctcttcaag
    34141 gctgatatcg gagagtttct ggacttttgc caccccggcg ctaccggcat agccgctgag
    34201 acgctcgtta cgcgccaccg ttggcgacgc agcaagacgc acctcggtga tggtgttcca
    34261 ggcatgacag gcactgcact gcccctgcca gcgcggataa tcggccccgc attcattaca
    34321 aacaaaggcg cgttttggag cttttgccac ggtgtacctc gttaatgctg tgccgcccgc
    34381 aggatgacgg gcaggcaatt acttctgatt caggctgcca gagaggatgc agaatacccc
    34441 catcaggtca gcgtgacgga tggtgacttc cgccttttca ttcacttttg gcttggcatg
    34501 gtaggcaatc cccagccctg ctgctttgat catcggcagg tcattagctc catcgccaat
    34561 cgccacggtc tgcgccagcg ggatttcata ctcctgcgcg aggcgagtca gagttttcgc
    34621 tttgtactgc gcgtctacga tgtcgccgat cacattgccg gtaaatttac cgtccatgat
    34681 ctccagttca ttggctacca cggctgtcag gcgcagcttg tcgcgcaggt attcagcaaa
    34741 gaaagtaaag ccgccggagg caatcgccac tttccagccc agcgtttcca gcttgagcac
    34801 cagctgcgtt aagcctggca tcagcggcag attttcacgc acctgttgca gaatattggc
    34861 gtcagcgcct ttcagcgtcg ccacgcggct gcgcaggctg gcggtaaaat cgagttcgcc
    34921 gcgcatcgcc cgttcggtta cctccgccac catctcgccc gttccggcca gcttggcaat
    34981 ttcatcaata cattcaatct ggatggcggt ggaatccatg tccatcacca gcaaacccgg
    35041 cgtgcgcagg tgcgggattt tccccagcgg ggcgacatcc agctgcgctt cgtgggccag
    35101 gcgtgtagcc cgtgcggtga gtgaacctgc cagacgaatc acctgataat cttccacgca
    35161 ccaggcggca acaatcacca tcgccgcacc cagtttgctc tggtattggg tcagacgctg
    35221 tttatccagc ccacgaccat acagcagcca gccgctacga cctgcgtggt aatccagtgg
    35281 catcacttca tcaccactta atgaaagagg cagacccggc cataaagaga catcttcagg
    35341 caggtcgcac caggtaatgt taggcattaa ggctcctgta aaatcgttcg aagcagggaa
    35401 aataacgcat gaggctacct tgtatccatt gcttctggca acattaagtc tcaaattttc
    35461 aaagggtgga agatggctcg cacaaaactg aaattccggc tacatcgggc agtgattgtc
    35521 ctgttctgtc ttgccttgtt agtggcactg atgcaggggg cgtcatggtt tagtcaaaac
    35581 caccagcgac agcgtaatcc acagctggaa gaactggccc gcaccctggc gcgtcaggtg
    35641 acgctgaacg ttgcaccgct gatgcgtacc gactcaccgg atgaaaaacg cattcaggcg
    35701 atcctcgatc agttaacaga tgaaagccgt atcctcgacg cgggtgtgta tgacgaacaa
    35761 ggcgatctta tcgcacgttc tggcgaaagc gtcgaagtgc gcgatcggct ggcgctcgac
    35821 ggtaaaaaag caggcggcta ttttaaccag cagattgtcg agccaattgc gggtaaaaac
    35881 ggaccgctcg gctatctgcg cctgacactc gacacccata cgctcgccac cgaagcccaa
    35941 caggtggata acaccactaa cattttacgc ctgatgttgc tgctctcact ggcaatcggc
    36001 gtagtgctga cccgcacgct gctacagggt aaacgcaccc gctggcagca atcgcccttc
    36061 ctgttaaccg ccagcaaacc ggtgccggaa gaggaagaaa gcgagaaaaa agagtgaccc
    36121 attactacaa gaaaggaaat cgttatgtcc acattacgcc tgctcatctc tgactcttac
    36181 gacccgtggt ttaacctggc ggtggaagag tgtatttttc gccaaatgcc cgccacgcag
    36241 cgcgttctgt ttctctggcg caatgccgac acggtagtaa ttggtcgcgc gcagaacccg
    36301 tggaaagagt gtaatacccg gcggatggaa gaagataacg tccgcctggc gcggcgcagt
    36361 ggcggtggcg gcgcggtgtt ccacgatctc ggcaatacct gctttacctt tatggctggc
    36421 aagccggagt acgataaaac tatctccacg tcgattgtgc tcaatgcgct gaacgcgctc
    36481 ggcgtcagcg ccgaagcgtc cggacgtaac gatctggtgg tgaaaaccgt cgaaggcgac
    36541 cgcaaagtct caggctcggc ctatcgcgaa accaaagatc gcggcttcca ccacggcacc
    36601 ttgctactca atgccgacct cagccgcctg gcaaactatc tcaatccgga taaaaagaaa
    36661 ctggcggcga aaggcattac ttcagtgcgt tcccgcgtga ccaacctcac cgagctgctg
    36721 ccggggctca ccatgagcag gtttgcgagg ccataaccga ggcctttttc gcccattatg
    36781 gcgagcacgt ggaagcggaa atcatctccc cggacaaaac gccagacttg ccaaacttcg
    36841 ccgaaacctt tgcccgtcag agtagctgag agtggaactt cggtcaggct ccggcattct
    36901 cgcatctgct ggatgaacgc tttagctggg gcggcgtgga actgcatttc gacgttgaaa
    36961 aaggccatat cacccgcgcc caggtattta ccgacagcct caaccccgcg ccgctggaag
    37021 ccctcgccgg gcgactgcaa ggctgcctgt accgcgcgga tatgctgcaa caagagtgcg
    37081 aagcgctgtt ggttgacttc ccggaccagg aaaaagagct acgggagttg tcgacgtgga
    37141 tagcgggggc ggtaaggtaa ctcgctgatt aaaatatcca aggaaggaaa tatgatcgat
    37201 ctggaaaacc aggaacggga gattatcaat ttaatgttca gccagcgtat ttcctggcta
    37261 gccgccgtgc gtatcaggca taaattatcg cttgctgaag taagtaaaat gttaggtatc
    37321 tcgattaatt ctctaaaaca gatcgaaaag acggaacggc ttagctcaaa catcaaaagc
    37381 aaaatggcag aaatttatgg ttgtccacca gagttgctga tatgcccttc ctggatgacg
    37441 gctgaacata aatgaataga ttcgtgaata gatacttgaa tgttttttat tttctatcca
    37501 tatgaaaaat aatagtaaat ctataaatca gttgctattg atgaatagat actaaaaagc
    37561 cggagcagtc ccccactccg gcttaacacg acaatcgcct ttcagcgaaa cacaattact
    37621 ctttatcgcc cagcagaacg gattccagtg cgattttgat catgtcgttg aaggtggtct
    37681 gacgctcagc ggcagtggtc tgctcgtgag tgcggatgtg gtcagacacg gtgcagatgg
    37741 tcagggcttt cgcgccaaac tctgcagcca cgccgtagat accagccgct tccatttcca
    37801 cgccgaggat gccgtatttt tccatcacgt cgaacatttc gccgtccgga gagtagaaca
    37861 ggtcagcgga gaacaggtta cccacgcgag catcaacgcc cagtgcttta gctgcatcta
    37921 ctgcgttacg caccatgtca aagtcagcga tagcggcaaa gtcatggtct ttaaaacgga
    37981 tgcggttaac tttggaatcg gtgcaggccc ccataccgat aacgacgtcg cgcagtttta
    38041 cgtgcggcag aactgcgcca caggaaccaa cgcggataat tttcttcacg ccgaaatcgg
    38101 tgatcagttc tttggtgtag atggagcagg acgggatacc cataccgtga cccattacgg
    38161 aaattttgcg gcctttgtaa gtaccagtga agcccagcat accgcgaacg ttgttcactt
    38221 cacgggcatc ttcaaggaaa gtttcagcaa tatacttcgc acgcagcggg tcgcctggca
    38281 tcaaaactac gtcagcgaaa tcgcccattt ctgcattaat gtgtggggta gccattgttt
    38341 tatccttttt gtgacataac aaaggcggag tcaacgctcc gcccaaatcc atcagaacat
    38401 ggctttgcca tattccatat cagaagtacc aaaatatttt gccagagtct ggccgatatc
    38461 cgcgaaggtt tcacggtgac ccagtgagcc cggttttact ttcgggccat ataccagtac
    38521 cggaatgtgt tcacgcgtgt ggtcagtacc ggtccaggtc ggatcgcaac cgtggtcagc
    38581 ggtgaggatc aggatgtcgt catcgcgcag cagagacatc agctccggca gacggcggtc
    38641 gaacagttcc agacccgcag cataaccggc gacgtcgcgg cggtggcccc aggaagagtc
    38701 gaagtcaacg aagttggtga agacgatggt gttatcaccc gcttctttca tctctttgat
    38761 ggtggcgtca aacagcgcgt ccaagccagt cgctttcact tttttggtga taccgcagtt
    38821 ggcgtagatg tctgcaattt taccgacaga aaccacctgg ccgtgttttt catcaaccag
    38881 tttctgcagc acggtcggtg ctggcggctc aacagccaag tcgtgacggt taccggtacg
    38941 ctggaagtta ccagctttgt caccgataaa cggacgagcg ataacacgac cgatattgta
    39001 gccgccgttg gtcagctctt cacgggcgat ttcgcacagt tcgtagagtt tatccagacc
    39061 gaaagtttct tcatggcagg caatctggaa cacggagtca gcggaggtat agaaaatcgg
    39121 cttgccggtt ttcatgtgct cttcgcccag ttgatccaga atgaccgtac cggaagagtg
    39181 gcagttaccg aggtaacccg gcagattagc gcgttcgacc agtttatcca gcagctcttg
    39241 cgggaagctg ttttcgtgat cggagaaata tccccactca aacagaaccg ggacaccggc
    39301 gatttcccag tgaccagacg gggtatcttt accggatgac atttcgtgcg cccatgcgta
    39361 cgcgccgata acttcagcgt tgccgtccat tcccgccgga atgaaaccgg tagaaccttc
    39421 gtgtgctttc gccagcccca gacgggtcag atttggcaga ttgagcggcc ctttacgacc
    39481 attatcagct tcgcctttgg cacaagcttc tgcgatatga cccagggtgt cagccccgac
    39541 gtcaccaaag cgttctgcat cttctgtagc gccgatgccg aatgagtcca gcaccataat
    39601 aaatgcacgt ttcatatgtt ctccgtactc agtgcttcaa atttaaatga tcagatcagt
    39661 ataccgttat tcgctgatac ggcgatagac agttggtgtg ctttccggtg cgttatcggc
    39721 aagtttaatt gccgctttca ccgctttcgc cgcttcctgc cagctgtttt cgtctttcgc
    39781 gaggataacc gccagcggac gctgaccgtc tacctggtcg cccagacgcg ccatatcagt
    39841 aaagccgacg ctgtaatcga tggtgtcaga tgcctgacga cgaccgccgc ccattgcaac
    39901 tactgccatc cccagcgcgc gggtatccat ttcactgaca aaaccttcgg tatcagcata
    39961 gactgctttc gtcagcatcg ctgtcggcag atacttcgcg tagttctcaa cgaagtcggt
    40021 cgggcctttt tgtgccgcta ccatacgacc aaagacttct gccgctttac cgttgtccag
    40081 caccgcctgc aatttcgcgc gcgcttcggc gtcatctttc gccagtttgc cggagatcaa
    40141 catctccacg cacagcgcca tcgtgacatc aaacagacgc gggttacgat actcacccgt
    40201 caggaactgc accgcttcac gaacttcaac cgcgttacct gcactggagg ccagtacctg
    40261 attcatgtcg gtgagcagcg cggtggtgcg cacgccagcg ccgttagcca cgccaacaat
    40321 cgcttcggca agggcttcag agagttcgta ggtcggcata aacgcgccgc tacccacttt
    40381 cacgtccatc accagcgcgt ccagaccttc cgccagtttc ttcgccagga tcgaggcagt
    40441 gatcagcggg atggagtcca cggttgcggt aatatcacgg gtcgcgtaga aacgtttatc
    40501 agccggagcc agtgaactgg tctgaccgat aatcgccacg ccgacgtctt taataatttc
    40561 gcggaaacgg ttgtcatccg ggaaaatgtc gaagccaggg atggattcca gtttgtcgag
    40621 cgtaccgcca gtatgaccga ggccgcgacc ggagatcatc ggaatatagc cgccgcaggc
    40681 tgcgaccatc ggccccaaca tcagcgaagt gacatcgccg acgccgccgg tcgagtgttt
    40741 atcaacaatc gggccattca gatgcaggct tttccagtcg agaacggttc ctgaatctcg
    40801 catcgccatg gtcagcgaga cacgctcagg cattgtcata tcgtggaaga aaatggtcat
    40861 cgcgagggcg gcaatctgcc cttcggaaat agtgttgtcg cgaataccgt tgataaagaa
    40921 acgaatttct tcatcgctca gcgcatgacc atcacgtttt ttacgaataa tttcttgtgc
    40981 gagaaacaag gtaacctccc gaggaaaaga gtatgaggcg gcttttgccg gatgcgtcta
    41041 cgccttatcc ggcctacggg tagatacgtc tcgtaggcct gataagcgca gcgcatcagg
    41101 cgtaaagcat cttacttagt agctgctggc gctcttaccg tcgccgtggc ccagcgcttt
    41161 cagcaggctt gccagcaggc tggaagcacc aaagcggtag tgacgcgcat cagcccagtc
    41221 agcaccgaac agttcatctg cgatggcgag atatttctgc gcatcttccg cagtacgcac
    41281 gccgcccgcc ggtttgaaac caacggtttt ttctacgccc atatcacgga tcacttccat
    41341 catgatgcga gcgctttccg gcgtcgcgtt cacagccact ttaccggtag aggttttgat
    41401 gaagtccgca cccgctttga tggagatttc agacgcttta cggatcagcg cttcgtcttt
    41461 cagttcgccg gtttcgatga tcactttcag cagtacattc gctgccgcgc aagcctcttt
    41521 acaggctttc accaggtcaa aaccaacctg ctcgtcaccc gccatcagcg cgcggtacgg
    41581 gaacaccacg tcaacttcat cagcaccgta ggcgattgcc gcacgggttt ctgccagcgc
    41641 gatttcgatg tcgtcgttac cgtgtgggaa gttggttacc gtagcgatac ggatttccgg
    41701 ggtgccctgc tctttcagag ttttgcgagc aatcgggata aaacgaggat agatacagat
    41761 agcggcggta ttgccgaccg gagttttggc ctgatgacac agagcaatta ctttctcgtc
    41821 ggtgtcgtcg tcattcaggg tggtcaggtc catcaatttc agtgcacgca ggctgcttgc
    41881 tttcagatca gtcatttcat tctcctggct tgtcgccaat aaaattcacc ttgcgagttt
    41941 gttagtattc taacatctac tccgcaacac acttcgatac acatcacaat taaggaaatc
    42001 tacttacaag tttgcatcac tgtaatgcga tctggttcaa ataattcact ttcaaatgaa
    42061 tgcgtcagtg gtggcaaacg catcaggatc aaagtgaaca tcacgaaact tcttacaatg
    42121 gcgcatcacc ttcggcataa aaggaaaaga agatgcccac ttctcatgaa aatgcactgc
    42181 aacaacgttg ccagcaaatt gtcaccagcc cagtgcttag cccggagcag aagcgccatt
    42241 ttctggcact ggaagcagaa aacaatctgc cttacccaca gcttcctgcc gaagcccgcc
    42301 gcgcgctgga tgaaggtgta atctgcgata tgtttgaagg tcatgcgccg tacaaaccgc
    42361 gctatgtctt acccgattac gctcgttttc tggcgaacgg ttccgaatgg ctggagctgg
    42421 aaggcgcgaa agatcttgat gacgcactct ctctgctgac cattctttac caccacgtac
    42481 cgtcggtcac atcgatgccg gtctacctgg ggcaactgga tgcgttgttg caaccgtatg
    42541 ttagaattct aacacaaaac gagatcgatg ttcgaataaa acgtttctgg cgttacctcg
    42601 acagaaccct gccagatgcc tttatgcacg ccaatatcgg cccgtctgat tcgcccatta
    42661 cccgcgcgat cttacgtgca gacgcgcagg tttcaccgaa cctgaccttt atctacgatc
    42721 ctgaaatcac ccctgatgac ctgctgctgg aagtggcgaa gaacatctgt gaatgtagca
    42781 aaccgcacat cgccaacggt ccggtgcatg ataaaatttt cacaaaaggg ggctacggga
    42841 ttgtgagctg ttacaactca ctgccgctgg cgggtggtgg cagcacgctg gtacgcctta
    42901 acctgaaagc cattgccgag cgcagcgaat cgctggatga cttctttacg cgcactctac
    42961 cgcactactg ccagcagcag atcgccatca tcgatgcgcg gtgtgaattc ctctatcaac
    43021 aatcacactt ctttgagaat agcttcctgg tgaaagaagg gctgattaac cctgaacgtt
    43081 ttgtgccaat gtttggcatg tatgggctgg cggaagcggt taacttgctg tgtgagaaag
    43141 aagggattgc cgcgcgctac ggtaaagaag ccgccgcaaa tgaagtgggt tatcgcatca
    43201 gcgcgcaact ggcggagttt gtcgccaata cccccgtgaa atatggctgg caaaaacgcg
    43261 ccatgttaca cgcacagtcg gggatcagtt ccgatatcgg caccacgccg ggcgcgcgtt
    43321 tgccgtatgg cgatgagcca gatccgatca cccatctgca aactgtcgcc ccgcatcatg
    43381 cttattatta ttccggcatc agcgacattc tgacgctcga cgaaaccatc aaacgtaacc
    43441 cgcaggcact ggtacagctt tgcctcggtg cctttaaagc cggaatgcgt gaatttaccg
    43501 ccaatgtcag cggtaacgat ctggttcgcg ttaccggtta tatggtgcgt ttgtcggatt
    43561 tagaaaaata tcgcgccgaa ggttcacgca ccaacaccac ctggctgggc gaagaagccg
    43621 cacgcaacac tcgtattctg gaacgccagc cgcgcgtgat aagccatgaa cagcagatgc
    43681 gctttagtca gtaagattat ccccttctcc tgcgttgacg ggccaggcag tcgtctggct
    43741 ctgtttttgc agggctgcaa tctgcgctgc aaaaactgtc acaatccgtg gacgatggga
    43801 cgttgcaatg actgtgggga gtgcgtgcca cagtgtccgc atcaggcgtt gcagattgtt
    43861 gacggcaaag tggtgtggaa cgctgtggtc tgcgagcaat gtgatacctg cctgaagatg
    43921 tgtccgcaac atgccacacc aatggcgcaa tccatgagcg tggacgaagt gcttagccat
    43981 gtgcgcaaag cggtgctgtt tattgaaggg ataacggtaa gcggcggcga agccacgacc
    44041 caactgccgt ttgtagtggc gctgtttact gctatcaaaa acgatccgca actgcgccat
    44101 ctcacctgtc tggtggacag taacggtatg ttgagcgaaa ccggctggga aaaattactc
    44161 ccggtgtgtg acggcgcaat gctcgatctc aaagcatggg gtagcgaatg tcatcaacaa
    44221 ttaaccggac gcgataatca acagattaag cgcagcatct atttgctggc agagcgcggc
    44281 aagctggcgg aactgcgttt gctgatgatc ccaggccagg tggattattt gcaacacatc
    44341 gaagaactgg cggcgtttat caagggactt ggtgatgttc cggttcgcct gaacgcgttt
    44401 catgcccacg gcgtgtatgg cgaggcgcaa agctgggcga gcgccacgcc ggaagacgtt
    44461 gagccgttgg ctgatgcatt aaaggtgcgc ggggtgagcc ggttgatatt tccggcgctc
    44521 tatttgtgac atgccggata cggcctacgg ttcggcacag acttgtaggc ctgataagac
    44581 gcgtcaagcg tcgcatcagg cattgtgcgc caactgccgg atgcggcgtg aacgccttat
    44641 ccggcctacg gttcggcaca aacctgtagg catgataaga cgcgttaagc gtcgcatcag
    44701 gcattatgcg ccgactgccg gatgctgcct acgaagggca aactacccac gaacgttaaa
    44761 caccgcatac gtgttattaa gcaacacttc ggcaatctca tccgcgggtt ccgggcgtaa
    44821 ctcgcaaagc acggcaaaca cacgggcagc ctgttccggg cggttaggct gcccctgaaa
    44881 accgttgagc ggcatatccg gcgcatcggt ttccagcaac aacgatgcca gcggtaattt
    44941 tgcgatgaca tcgcgggttt tactggcgcg tggataggtg atagtaccgc ctacgccgat
    45001 tttgtagccc agctgtacaa accgttcagc ctgttgctgg ctgccagaaa atccgtggac
    45061 aacaccagca cgcggcaaat cgtggcgttt aagatgcatc gccagtttgt cgtgcgtgcg
    45121 ccgtgaatgc aggatcaccg gcagatcgta gcgtttcgcc agtttcagtt gttcgtcgag
    45181 taaccactgc tgcctctcaa attgcggatc gtcgccgaag agatccagac cgatctcccc
    45241 caccgccacc accttcgccg gacgcctttc cagcgcctgc tgtagctgct caagagacac
    45301 atcgctatgt ttttccaaca taccaggatg caagcccaat acggcataca gcggttgata
    45361 attttccgct aacgcctgca cacgggcaaa attttccgcc tctgtcgccg gaacaatgat
    45421 cttgcctacg cccgcttgtg ccgcgcgttg caggctggcc tcttcatcgc caataaaagg
    45481 cgggaaatca aaatggcagt gagtgtcgat aaaacggcaa atcaagccag atcctcattg
    45541 ttaaatgtcg ggtcgttcgc ctgcggagcg tcgctcactt cggcaaccag cgtatcgttg
    45601 gcgactggcg caggcggaat gacaatcgat tccggcgtca ccactggcac cagatggcgg
    45661 gtaagcggcg ctttttcagt taatagcttg ccaaccgtgg cgaggaaata acgaccgcat
    45721 aaacgcccaa gtttatagtc ttcacgcagc gccggaatcc gactgccaag cgcgatacta
    45781 tgtaatggct tcggcggata aatttcggat atccgcagct tgcccggtgg tttctcaata
    45841 aattgctgaa tgtcacgata gctggtttca tgatgctgca ccagattgac cagcggctgc
    45901 aggctactgt cacccagcca gcgttccatg cgtttgaacc actgcggcgt gtagtacatt
    45961 tgtgacggca cagtgcggat gacgaccaac gttttagcgc cctgccttgc cgcctcttta
    46021 accggaatcg catcactgat cccgccatcc aggtagttaa tgccttccag cgacactccg
    46081 ctacgataaa agccaggtat cgcactggag gcgcgaatca catccagcca gttttgtttg
    46141 gttggtaaaa agtaattcgg cgcgtagtca tcctgacgac aggcgcacat ataaaacgat
    46201 ttgccgctgt caaacaaccg cgcggcggta tccatttgca acggcatctg gcttgcggtg
    46261 gcctccacca gccagtcgag atcgataaga tttcctccac ggacaaagcg caatggatcg
    46321 aaaaattcgc gttttgtggt atagcgcatg atgactttgc gcgcgtaacc gggctgattg
    46381 cagataaacg ccgagaggtt ctgcgccccg gcagatgtgc cgagataaag atcgaaagga
    46441 ttaaactgcg cgcgcataaa ctcatccagc acgccagccg tgaaaattcc acgctgtccg
    46501 ccgccttcgc acaccagagc tattcgtcca ggctggaacg gccttagcga caacggcgca
    46561 atattaccaa gcgttacagg tattcgctgc cccacttctg ccttcctgtt tttgttgtga
    46621 aagaaagaca aagtaacgca atttattatt cgctaaaacc gcaaaagcca gtccgctgga
    46681 ctggctttgg ataaaatttt cttaatggtc tgaaatctag ggtcgttttc ggcccatgaa
    46741 caaactcact aggaacagaa taatcccgac gacaaagaca attttagctg cgcctgcagc
    46801 ggtaccggcc agaccaccaa acccaagtgc ggcggcgatt aacgcgataa ccagaaatat
    46861 gatgccccaa cgaaacataa gcgtctcctt taccatagtt aatgtcaccg cttgatatga
    46921 gcgtgaaaat cactcaatgc gatattttta gtgtggtgca catcgcgcct cccgacaaag
    46981 ttcgggagga cgaattactt agttttcaga tcatttttaa cgcttttcac accatctacc
    47041 gctttggcga tactttcagc acggtcactt tgtgcctgag aatctacggt accggagagc
    47101 tgaaccacgc catcggtggt ttcaactttc actttacgtg aagggacgat atcgtccgcc
    47161 agcagtttgg ctttgatttc actggtggtg gcggtgtcac ccgcgtagcc cttcaccgag
    47221 ccttctttag cgtcgcgaac gtgcagtttg tcgctgacag aggtcacgcc ttcaacgcct
    47281 ttcgccactt tcactgcctc ttcggcctgg gcctgacttt caacgaaacc gctcagggtc
    47341 acgacttttt gatcggtttt gacagagata tcggtgctct tgatgttgtc atgatccacc
    47401 agcgccgcct tcactttcgc ggtgatggcg ctgtcatcca tgaaattacc gactttattc
    47461 atagagctat cgactttttg ccctgcgctt tcattggtag tctgcgcgtt gttttccgcg
    47521 taggcagagc cggtcgcgac ggcagaggtc aacattacag ccagcagagt tttcgaaatc
    47581 ttcagtcttg tcatagtcat cgatttattc ctgtatgttt gctcgtaatt tgagcctagc
    47641 aacacgaggt tgcattgctg aatagggaga gacttcaccc tctacagaag tcaatggtcg
    47701 ccatcacaaa agcgatgggt gatgaataac gaccattaca gcctctgaat cagttattaa
    47761 tatcggtaga atgacaatcg acggctttag atactaatat ctacgcattg aacggtattt
    47821 aacgccatca gaaatgtcat cactttgtta aatatagatc acaattttga aaccgctcgg
    47881 gatatcagcg aaaacataag caaaagtgaa tgttttaaga acattccgta agcggctaat
    47941 aaggaaggga aattgacagg gcgcagcggc taccgcgccc tggaggcaag aattaatgct
    48001 cgcgggtctg gtggaactga acgtccggat aacgttcctg tgccaggcgc aggttgacca
    48061 tgctggtagc gatgtaagcg aggttatcgc cgccatcaag cgccagttgg ctttcgttct
    48121 tacgcttgaa ctcttcgaat ttcttcgcgt ctgcacattc tacccagcgg gcagtggcaa
    48181 cgttgactga ctcatacact gcttcaacgt tgtattcgct cttcaggcgc gctaccacca
    48241 catcaaactg cagcacacca accgcaccaa cgatcagatc gttgttggag attggacgga
    48301 acacctgcac cgcgccctct tcggaaagct gtaccagccc tttgagcagc tgtttttgct
    48361 tcagcggatc tttcaggcgg atacgacgga acagttctgg tgcgaagttc ggaataccgg
    48421 tgaacttcat catctcaccc tgggtaaagg tgtcgccgat ctgaatggtg ccgtggttgt
    48481 gcaggccgag gatatcgccc ggatacgctt cttcaacgtg cgaacggtca cccgccataa
    48541 aggtcagcgc gtcggagatc accacatctt tcgcagtgcg cacctggcgc agtttcatgc
    48601 ctttttcata tttaccggac accacacgca taaacgccac gcggtcgcgg tgtttcggat
    48661 ccatgttggc ctgaatttta aatacgaagc cggtaaattt atcttcgctc gcttctacgg
    48721 tacgggtatc ggtctgacgc ggcatcggcg caggcgccca ctccaccagg ccattcagca
    48781 tatgatcaac gccgaagtta cccagcgcag taccgaagaa taccggagtg atttcgcccg
    48841 caaggaacag ctctttgtcg aactcgttag acgcgccttt aaccagttcc agttcgtcac
    48901 gcagctgctg tgccagatct tcaccaaccg cagcatcgag atccgggtta ttcagccctt
    48961 taacaatgcg gacttcctga atggtgtgcc ctttaccgct ctgatagaga taggtttcat
    49021 ctttataaag gtggtaaacg cctttaaaca gcttgccgca gccaattggc caagtgatcg
    49081 gtgcgcagcc aattttcagc tcgttctcaa cttcatcgag cagttccatc gggtcgcgga
    49141 tatcacggtc aagtttgttc ataaaggtga ggatcggcgt gtcgcgcaga cgggtaactt
    49201 ccatcagctt acgggtacga tcttcaacac cttttgcggc gtcgataacc atcaggcagc
    49261 agtccaccgc cgtcagggta cgataggtat cttccgagaa gtcttcgtgc cccggggtgt
    49321 cgagcaggtt aaccaggcaa tcgtgatacg gaaactgcat cacagacgta gtaatggaga
    49381 tcccacgctg cttttccatc tccatccagt ccgacttagc gtgctggttg gaaccacggc
    49441 cttttactgt accggcggtc tgaatggcct gtccgaacag cagcaccttc tcggtgatgg
    49501 tagttttacc ggcgtccggg tgagaaataa tggcaaaagt gcggcgcttc gccacctctt
    49561 tcaaataagg agacaacgtc ataattcttt cttcttgagt aagcggcatc gcgccgcgct
    49621 tgttgaaaac gaaaaattgc ggctatttta cccatcaacg ggggggaggc aatcagtgtt
    49681 tacacaggag ctgctccagt tcgtgcaacg aagaaacggt ccaggtgggc gcgatgcctt
    49741 ctggttgctc gcgattgtgc gcattcagcc agcaggtcgc aagcccggcg ttgatgccac
    49801 cgagaatatc ggactcggca gtgtcgccaa ccatcagcac gcgtgaacgg tcaggattgc
    49861 ccgcctgttc cagcgcataa tcgaaaattt tcttattcgg tttggcaacg ccaacttctt
    49921 cggaaatcac cagcaaatcg aagtaatcac gcaggcccgt ccgttccaga cgcacttgct
    49981 gcaaggcgct aaaaccgttg gtgatgatgc cgattttagc gttgctacga atggcgttaa
    50041 gcagagaaac cgcgcccggc aacggcgtac agatttccgc catcgcatta ataaaggcat
    50101 cgttaagcaa gcctgcttca actttcagcc gttcggccca gctctcgaaa cgcccgtgct
    50161 gaagctgtaa tgaagtgatc gcgccgtttt gataatccac ccacagtggc ttgttaacgg
    50221 cctgatagtc ctgaaaatct tcagcggtaa aggtgacgct gtaatcaaga aacatccgct
    50281 gcaggccggt gaatgagtca aaggtaaaca gcgtttcatc ggcatcaaag aaaatccagt
    50341 cccacttcat tattccacct tgtcttacat actgattggc aacgccatga tgatggcgtc
    50401 ttcgcgaccg tccgtggtgg ggtagtaatt gcggcgaatc gtcgcctcgt taaagcctaa
    50461 actttcgtac agggcaatgg cggcagcatt tgaggcacgg acttccagcc atagtgtcgc
    50521 cacgccgcgt ttttccagtt cgtcgatcag atgttccagc agcgccctac ccagcccctg
    50581 acgctgatag tcgggatcga ccgcaatatt gaacaatgta gcttcatcca gcaccacttg
    50641 cgtaatcgca aacgccgcca ttttgccgtt ttgcgttaac tgaaagttga gataacgctc
    50701 gccctggttg ctggcaaagg ttttttcact ccacggaaag gcgtgggcgc gttgttcaat
    50761 gtggtaagcc gctggtaaat cagtcgtttc gagggaagaa atcgtgttca tatgtgcaaa
    50821 tttgttgcca taacgcggcg cgtgccgttg ggtttgcccg taattcggtg agcgccggtg
    50881 atgccacctg agcgccttcc aatgatagcg gttcgtcagt acccaaccgc cagctgttgc
    50941 agcgactgcc ttgcggcagc atcgcgattt tttctggcgt cagttgcagc acttgatcgg
    51001 ggctgacggt taatgcgcgc agaacatcgc tcactaaagg atcagtcagg gcgggaagat
    51061 cgtttgccac catcaccaga cggacgtgtg ccgggctcgc aatggcaatc tccccctgca
    51121 acgcgccagg gcgacgcagc gaccactggg taatgcccag ttgctgtaac tgccagtctc
    51181 gtcgggatgt catagcgaat cgctcctgtt gtcaggggcg caaatatagc aaattcgtcg
    51241 ataccgcgcc aacatatggc tataatcgcc gccagtatca attgaggagc attccatgtc
    51301 tgcatttacc ccggcaagtg aagtcttgct gcgtcacagt gatgatttcg aacaaagccg
    51361 tattctgttt gccggagact tacaggatga cctgcccgcg cgtttagata ccgcggccag
    51421 ccgtgctcat acccagcaat tccaccattg gcaggtgtta agctgccaga tgggggataa
    51481 cgcccgtttt agtctggtcg ccacggcgaa tgatgtcgca gattgcgata cgctgattta
    51541 ctactggccg aagaacaaac cggaagccca gttccagttg atgaatttac tttctctgct
    51601 gccagtgggg acagatattt ttgtcgttgg cgagaaccgc agcggcgtgc gcagcgctga
    51661 gcagatgctg gcagattatg cgccgttgaa taaagtcgac agcgctcgtc gctgtggcct
    51721 ctattttggt cgtctggaaa aacagccagt atttgatgcg gataaattct ggggcgaata
    51781 cagcgtcgat ggcctgacgg tcaaaacgct gcctggcgtg tttagccgcg acggtctgga
    51841 tgtcggtagc cagttgctgc tctcgacgtt aactccgcac acgaaaggta aagtgctgga
    51901 tgtcggctgt ggcgcgggtg tactttcagt tgcctttgcg cgccattcgc cgaaaattcg
    51961 tctcaccttg tgcgatgtct ctgccccggc ggtagaagcc agccgcgcaa cacttgcagc
    52021 caacggtgtt gaaggtgaag tctttgccag caacgtcttt tccgaggtga aaggttgttt
    52081 tgatatgatc atctccaacc cgccgttcca cgatgggatg caaaccagcc tggatgcggc
    52141 gcaaacgctg attcgtggcg cggtgcgtca tcttaatagc ggcggcgagc tgcgaattgt
    52201 agcgaacgcc ttcctgcctt acccggacgt gctggatgag acatttggct tccatgaagt
    52261 tatcgcgcaa accgggcgct tcaaggtata tcgcgccatt atgacccgcc aggcgaagaa
    52321 aggttgagta tctgatatct gtcgccgcat accgtgtcgg atgcggcgtg aacgccttat
    52381 ccgacctaca aaacctgcac gttagccctt cgtaggccag ataagacgcg ccagcgtcgc
    52441 atctggcatc tgcccccgca tgcctaccaa acctgcacgc tagtttcctg atggacattt
    52501 ttccagcaat tacacatcag gcgataatta actattgacg aaaagctgaa aaccactaga
    52561 atgcgcctcc gtggtagcaa ttctttttaa gaattgatgg
//
LOCUS       DAJTUI010000019        49820 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTUI010000019 DAJTUI010000000
VERSION     DAJTUI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.019"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..1224
                     /locus_tag="OO464_001201"
     CDS             <1..1224
                     /locus_tag="OO464_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6226423.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="OO464_001202"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="OO464_001202"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6226424.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="OO464_001203"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="OO464_001203"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR6226425.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="OO464_001204"
     CDS             2714..3427
                     /locus_tag="OO464_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6226426.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="OO464_001205"
     CDS             complement(3399..4076)
                     /locus_tag="OO464_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6226427.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="OO464_001206"
     CDS             complement(4070..4747)
                     /locus_tag="OO464_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6226428.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5118)
                     /locus_tag="OO464_001207"
     CDS             complement(4735..5118)
                     /locus_tag="OO464_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6226429.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5134..5442)
                     /locus_tag="OO464_001208"
     CDS             complement(5134..5442)
                     /locus_tag="OO464_001208"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226430.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5443..6021)
                     /locus_tag="OO464_001209"
     CDS             complement(5443..6021)
                     /locus_tag="OO464_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6226431.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="OO464_001210"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="OO464_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="OO464_001211"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="OO464_001211"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6226432.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="OO464_001212"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="OO464_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6226433.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="OO464_001213"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="OO464_001213"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6226434.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="OO464_001214"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="OO464_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6226435.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="OO464_001215"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="OO464_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR6226436.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="OO464_001216"
     CDS             12291..13031
                     /locus_tag="OO464_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6226437.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="OO464_001217"
     CDS             13198..14148
                     /locus_tag="OO464_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6226438.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="OO464_001218"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="OO464_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="OO464_001219"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="OO464_001219"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6226439.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="OO464_001220"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="OO464_001220"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6226440.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="OO464_001221"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="OO464_001221"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR6226441.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="OO464_001222"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="OO464_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6226442.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="OO464_001223"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="OO464_001223"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6226443.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="OO464_001224"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="OO464_001224"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6226444.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="OO464_001225"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="OO464_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6226445.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="OO464_001226"
     CDS             23768..23827
                     /locus_tag="OO464_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6226446.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="OO464_001227"
     CDS             complement(23928..24530)
                     /locus_tag="OO464_001227"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6226447.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="OO464_001228"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO464_001228"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="OO464_001229"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="OO464_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6226448.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="OO464_001230"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="OO464_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6226449.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="OO464_001231"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="OO464_001231"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6226450.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="OO464_001232"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="OO464_001232"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6226451.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="OO464_001233"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="OO464_001233"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6226452.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29676..29759)
                     /locus_tag="OO464_001234"
     CDS             complement(29676..29759)
                     /locus_tag="OO464_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226453.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30050..31144
                     /gene="gcvT"
                     /locus_tag="OO464_001235"
     CDS             30050..31144
                     /gene="gcvT"
                     /locus_tag="OO464_001235"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6226454.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31168..31557
                     /gene="gcvH"
                     /locus_tag="OO464_001236"
     CDS             31168..31557
                     /gene="gcvH"
                     /locus_tag="OO464_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6226455.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31676..34549
                     /gene="gcvP"
                     /locus_tag="OO464_001237"
     CDS             31676..34549
                     /gene="gcvP"
                     /locus_tag="OO464_001237"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6226456.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34816..35559
                     /locus_tag="OO464_001238"
     CDS             34816..35559
                     /locus_tag="OO464_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6226457.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="OO464_001239"
     CDS             complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="OO464_001239"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR6226458.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37094..37404
                     /locus_tag="OO464_001240"
                     /pseudo
     CDS             37094..37404
                     /locus_tag="OO464_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37568..38227
                     /locus_tag="OO464_001241"
     CDS             37568..38227
                     /locus_tag="OO464_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6226459.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="OO464_001242"
     CDS             complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="OO464_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6226460.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39646..39912
                     /gene="sdhE"
                     /locus_tag="OO464_001243"
     CDS             39646..39912
                     /gene="sdhE"
                     /locus_tag="OO464_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6226461.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39893..40300
                     /locus_tag="OO464_001244"
     CDS             39893..40300
                     /locus_tag="OO464_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR6226462.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="OO464_001245"
     CDS             complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="OO464_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6226463.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40973..41869
                     /gene="xerD"
                     /locus_tag="OO464_001246"
     CDS             40973..41869
                     /gene="xerD"
                     /locus_tag="OO464_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6226464.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41894..42604
                     /gene="dsbC"
                     /locus_tag="OO464_001247"
     CDS             41894..42604
                     /gene="dsbC"
                     /locus_tag="OO464_001247"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6226465.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42610..44343
                     /gene="recJ"
                     /locus_tag="OO464_001248"
     CDS             42610..44343
                     /gene="recJ"
                     /locus_tag="OO464_001248"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6226466.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44434..45532
                     /gene="prfB"
                     /locus_tag="OO464_001249"
     CDS             join(44434..44508,44510..45532)
                     /gene="prfB"
                     /locus_tag="OO464_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6226467.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45542..47059
                     /gene="lysS"
                     /locus_tag="OO464_001250"
     CDS             45542..47059
                     /gene="lysS"
                     /locus_tag="OO464_001250"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6226468.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47102..47650)
                     /gene="idi"
                     /locus_tag="OO464_001251"
     CDS             complement(47102..47650)
                     /gene="idi"
                     /locus_tag="OO464_001251"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6226469.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47705..47776
                     /locus_tag="OO464_001252"
     CDS             47705..47776
                     /locus_tag="OO464_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226470.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47773..47898)
                     /locus_tag="OO464_001253"
     CDS             complement(47773..47898)
                     /locus_tag="OO464_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226471.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="OO464_001254"
     CDS             complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="OO464_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6226472.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12050 a        12449 c        13402 g        11919 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caagcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tctaatacca tgattttttg atttagtgtt ttttgacatt
    29761 tttttagctc ttaatattgt cttattcaaa ttgactttct catcacatca tctttgtata
    29821 gaaattggtg tattttttgg ttttttattc tgtcgcgatt tttgcatttt ttaaccataa
    29881 gctaatgtga tgatcaattt taccttatgg ttaacagtct gtttcggtgg taagttcagg
    29941 caaaagagaa cgattgcgtt ggggaccggg agtggctccg atgctgggtt ttgtggtgat
    30001 aatttcacca tgaaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    30061 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    30121 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    30181 cggaatgttt gatgtgtcgc atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    30241 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    30301 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gatctcatcg tctactactt
    30361 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    30421 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    30481 gattgccgtg caagggccga atgctcaggc aaaagctgcc acactgttta atgatgctca
    30541 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggtgggcg atctgtttat
    30601 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    30661 ggccgatttc tggcgtgcgc tggtggaagc gggcgttaag ccctgcggtc tgggggcgcg
    30721 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    30781 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccgacag atcgtgactt
    30841 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    30901 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    30961 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    31021 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    31081 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    31141 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    31201 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    31261 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    31321 gttagcgcgg gcgatgactg cgcggttgcc gaatcggtaa aagcggcgtc agacatttat
    31381 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc cccggaactg
    31441 gtgaacagcg aaccgtatgc aggcggctgg atctttaaaa tcaaagccag cgatgaaagc
    31501 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga tgagtaacgg
    31561 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    31621 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    31681 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    31741 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggtgca caatcgttaa acgcgctgac
    31801 cggccagatt gtgccgaaag atattcagct tgcgacacca ccgcaggttg gcgcaccggc
    31861 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    31921 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    31981 gctggaaaat ccgggctggt ataccgcgta cactccgtat cagcctgaag tctcccaggg
    32041 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    32101 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    32161 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccaatg tgcatccgca
    32221 aacgctggat gtggtccgta ctcgtgctga aacctttggt tttgaagtga ttgtcgatga
    32281 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    32341 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    32401 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    32461 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    32521 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    32581 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    32641 gcaacatatt cgtcgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    32701 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    32761 ccgcattcac cgtctgaccg atattctggc ggcgggcctg caacaaaaag gtctgaaact
    32821 gcgccatgcg cactatttcg acaccttgtg tgtggaagtg gccgacaaag cgggcgtact
    32881 gacgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg ccgtggggat
    32941 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagtg tgctgctggg
    33001 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    33061 tatccagcct gcgatgctgc gcgacgacga aatcctcacc catccggtgt ttaatcgcta
    33121 tcacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    33181 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    33241 gatcccaatc acttggacgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    33301 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    33361 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    33421 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    33481 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    33541 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    33601 gggcgataac ctctcctgta tcatggtgac ttatccttct acccacggcg tgtatgaaga
    33661 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    33721 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    33781 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    33841 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    33901 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    33961 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    34021 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    34081 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    34141 gaaagaagaa accggcatca gcgagctgga cattgccaag cgcctgatcg actacggttt
    34201 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    34261 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    34321 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    34381 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    34441 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    34501 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa taccagtaat tcactgattc
    34561 gagtattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    34621 cagacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg
    34681 gcgcgaatat ttaccctcac cccgaccctc tccctgaaag ggcgaggggg aaaagcgtgc
    34741 caacattgaa gattgagcca gtttgttagc aatctcaaag atacgtcaac gaattaattt
    34801 ttctcggaaa aacaaatggc tatagcactt gtgaccggtg gcagtcgcgg catcgggcgg
    34861 gcaactgcat tactgttggc gcaagaaggg tatacggtgg cggttaatta tcagcaaaac
    34921 ctccacgcgg cgcaggaagt gatgaactta ataatgcaag ccggtggcaa agcattcgtg
    34981 ctccaggcgg atatcagcga cgaaaaccag gtcgttgcga tgtttacagc aatcgatcag
    35041 cacgatgaac cgctagcagc gctggtcaat aacgccggga tcttgtttac ccagtgcacc
    35101 gttgaaaacc ttaccgcaga gcgaatcaac cgagtacttt ccaccaacgt gacgggatat
    35161 tttctctgct gccgcgaggc ggtaaaacgc atggcgctta aaaatggtgg cagtggcggc
    35221 gctatcgtca atgtctcttc ggtggcctca cggttgggtt cgccagggga atatgttgat
    35281 tacgcggcat cgaaaggggc gattgattcg ttaaccaccg gactatcgct ggaagtcgcc
    35341 gcgcagggga tccgcgttaa ctgcgtgcgg ccagggttta tttataccga aatgcacgcc
    35401 agcggcggcg agcctggacg cgtcgatcgc gttaagtcga acatccccat gcagcgtggt
    35461 ggacaggcag aagaggtcgc gcaggccatt gtctggctac taagtgataa agcctcttac
    35521 gtcacgggaa gttttatcga tttggcgggc gggaaataaa acagggaagt tgtctgaccg
    35581 gatgcaacaa gtattgcatc cggtacttca tcgacttaaa gcttctcgcc gttgctggca
    35641 atcacctctt tgtaccagtt aaagctcttc ttacgtgaac gcgacatatc gccagtaccg
    35701 tcgtcatgtt tattcacata gataaagccg tagcgtttgc tgtactgccc ggtagtgaat
    35761 gacacgcagt cgatgcagcc ccacggtgtg tagcccatca gatccacacc atcgtaagtc
    35821 actgcttttt tcatctcttc aatatgggcg cgcaggtagt caatgcggta gtcgtcgttg
    35881 atgctgccat cttcttccac tttgtcgtaa gcgccaaaac cgttttcgac aataaacagc
    35941 ggcctctgat aacgctcata cagttcgcaa agggcatagc gcagacctac tggatcaatc
    36001 tgccagcccc agtcagacgc tttaacatac gggttcggta cgctgccttc aaaaccagag
    36061 atcgcatcgc cggtgccgcc ttcggccttc actgcgttgg tcatgtaata gctgaaacca
    36121 agataatcgc aggtgccttc gcgcagtaca tcgagatcgc cgtcttccat tttgatatta
    36181 aatccgcgac gttcccactc gttcaataca taggacgggt aatagccgca tagctgcaca
    36241 tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga acatcacatc gtccgggtta
    36301 catgagtagg gatagagcgg caccatcgcc agcatacagc cgactttcat ctccgggtta
    36361 atgcgacgcg cggctttcac tgccagggcg ctggcgacaa actggtgatg cagcacctgg
    36421 tacatcgtct cttccgggtt gtcatgctcg gtatacacca cgccggagca gcagtaacca
    36481 aacagcggcg cacgccagtt acgctggttg tttatttcgt tgaaggtcat ccagtatttg
    36541 actttatgct tgtagcgttc gaataccact tccgcgaaac ggacaaagaa atcaacgaat
    36601 ttacggttgg tccagctacc gtattgctgc accagatgca gcggcatttc aaagtgggag
    36661 agggttatca ccggttcgat gttgtatttc agcagttcat cgaacagaga gtcgtaaaac
    36721 ttcagccctt cttcgtttgg ctgagcttca tcaccttttg gaaaaatacg cgtccaggcg
    36781 atggaagtcc gaaaacattt gaagcccatt tcggcaaata gcttgatgtc ttccttgtag
    36841 tgaccataaa aatcaacggc ttcatggttt ggatagtatt ttcctggcaa gacttctttg
    36901 gtgatttcgc gcggcacgcc gtgtgcgcca caggtcagaa cgtcacaaat gctcggtcct
    36961 ttgccgcctt tgttccagcc gccttcgacc tgatgagcgg caacggcgcc accccataag
    37021 aaatctttcg gtaaggtgag ttttttcact atcataggct ccagatcagt caattcttac
    37081 aggaaatagt accatgcagc caaacgacat cacttttttc aacgtttcca ggatgacatt
    37141 ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt caaaacgggt
    37201 gatgtgcttc gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc
    37261 gcaacctcaa ccgtaacgct ggatacactg acggaaaaac atgcagagca ggaaaatatg
    37321 accctgcctg aactgaaaaa ggtcattgct gacatctatc ccggtcagac acaattttat
    37381 atgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttcgcattt
    37441 aatttcatga tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt
    37501 cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctctgga
    37561 gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc
    37621 caacagcgtc agtcacggca ttgggctggt gtttggtatc gttgggctgg tgttgctact
    37681 ggttcaggcg gtggatctca atgccagcgc cacagcgata accagttaca gcctctatgg
    37741 cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg
    37801 ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac
    37861 ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt
    37921 tatctggagc ctagcattgc tggggattct gttcaaactg accatcgcgc accgattcaa
    37981 aattttatct ctggtgactt atctggcgat gggctggctg tcgctggtgg taatttatga
    38041 aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta
    38101 ttcgctcggg gtgattttct acgtctgcaa acgcattcca tacaaccatg ccatctggca
    38161 cggcttcgtg ctcggcggta gtgtgtgcca ctttctggcg atctatttgt atattgggca
    38221 ggcgtaatgg gtatgccttt gtaggcctga taagacgcgt cagcgtcgca tcaggcatcg
    38281 gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat ctacgggcct cgtccctgtt
    38341 gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat caggcgataa
    38401 atgcctgatg cgatacagag atttactctt cgagcgaata cggcagcggc tcgatatgca
    38461 gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg ttattcatga
    38521 cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc gtaccggtac
    38581 gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg
    38641 cactacctgc cagcaaccag agcgcgcgtt tgttggcacc acggaatttt gctcgcgcca
    38701 ccatctcttg tccggtataa cagcctttct taaagctgat accgcccagc gcctggagat
    38761 tggtcgcctg tgggataaac tgcccgctgt tggcggcatc aatcaccggg aaaccagctt
    38821 caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat
    38881 cggtcagcat attagcagta gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt
    38941 caaaccatag caaagtggtc gcgccttctt tgactacctg tttttctttc gaaggcagtt
    39001 cgctaaagag atttgccagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac
    39061 gctcgtcgtc tggcgcgatg gtcactttag agaataccgc atattttttc agttcagtca
    39121 gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa gccatcgccg tcgcggaaca
    39181 gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat
    39241 cttctgccat ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg
    39301 cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg
    39361 cagaagccgt aggctgacgt ggaggaaaag gtgtaaaagc cataatagag tcctgagttg
    39421 cttaacggcg tattaatgac taatggtaaa agagctatga ggcaatgcaa gtgctttcac
    39481 tacgcgatct gtgccttctg cgaggcgact gccaaaaaaa tgaagccctt gaattgttgt
    39541 gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa gtcgaaaaac
    39601 acgttacaat agccgggtac tggattttcg tgagacagga agaagatgga cattaacaac
    39661 aaagcccgaa ttcattgggc atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg
    39721 tcgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat ctttattcgt
    39781 ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg taaaccggcc
    39841 gatgcagaac tggaaataat ggtccgactc atccagacac ggaaccggga acgtggtcct
    39901 gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc tgattcatgg
    39961 gctggttgcc gctgttattt tactcatgcc ctggccgctc agttacaccc cgttatggat
    40021 ggtgttactt tcgctggtgg tatttgattg cgttcgcagc cagcggtgta ttaatgctcg
    40081 ccagggggaa attcgcttgt tgatggacgg gcgtttgcgt tggcaagggc aggagtggag
    40141 catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac gttctgatgg
    40201 cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagccg aatggcggga
    40261 tttacggcgg attttgttgc aacaagagac gcaaagataa agatgacggc aaagcatatg
    40321 cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg atttgctcgc
    40381 accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc agcgccagac
    40441 ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca tatccttccg
    40501 ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc agcataccga
    40561 gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac agcgcaataa
    40621 ttttaccttc aaggttcagg tcgtcgagct gatcccagac ggcttcccag tcttcctgga
    40681 tttcaccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc tccattaatt
    40741 tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata atatcgcgga
    40801 ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga cccatattca
    40861 taacgtaagc ttctcgattc tgctgtggct tttgcagcta gtgtatcaga atcgctttag
    40921 tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc aagtgaaaca
    40981 ggagctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa atctggctga
    41041 aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg
    41101 cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg cagaacggct
    41161 ggagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt
    41221 ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc tcgcttcacc
    41281 gaaattgccc cagcgtttgc caaaagattt aagcgaagcg caggtcgaac gtttattaca
    41341 ggcgccatta attgatcagc cactggagct acgcgataaa gccatgcttg aagtgttgta
    41401 tgctaccggg ctgcgtgtct ctgaactggt cgggctgaca atgagtgata tcagcctgcg
    41461 tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtgc cgttagggga
    41521 agaggcggtt tactggctgg aaacctatct ggaacatggg cgtccgtggc tgctgaatgg
    41581 tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc gacagacctt
    41641 ctggcaccgc attaaacatt atgccgtgct ggcgggtatc gacagcgaaa agctgtcacc
    41701 gcatgtgttg cgtcacgctt ttgccactca tttattaaat catggtgcgg atttacgcgt
    41761 ggtgcagatg ctactgggcc acagcgatct ttccaccacg caaatttata cgcatgtcgc
    41821 taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag
    41881 aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg cgttttcagg
    41941 ctttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg gcatcaaaag
    42001 cagcgatatt cagcccgcgc ccgtagctgg catgaagaca gttctgacta acagcggtgt
    42061 gttgtacatc accgatgatg gtaaacatat cattcagggg ccaatgtatg acgttagtgg
    42121 cacggctccg gtcaatgtca ccaataagat gctgttaaag cagttgaatg cgcttgaaaa
    42181 agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt ttactgatat
    42241 tacctgtggt tactgccaca aactgcatga gcaaatggca gactataacg cgctggggat
    42301 caccgtgcgt tatcttgctt tcccgcgcca ggggctggac agcgatgcag agaaagaaat
    42361 gaaagctatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga tggcaggtaa
    42421 aagcgtcgca ccagccagct gcgacgtgga tattgccgac cattacgcac ttggcgtcca
    42481 gcttggcgtt agcggtactc cggcagttgt gctgagcaat ggcacacttg ttccgggtta
    42541 ccagccgccg aaagacatga aagaattcct cgacgaacac caaaaaatga ccagcggtaa
    42601 ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga aacggcagac
    42661 ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg agtacgcagt
    42721 gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact aagcggtgtc
    42781 gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat tattgtggtc
    42841 ggtgatttcg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg
    42901 cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg ttacggctta
    42961 agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt cacggtggat
    43021 aacggtattt cctcccatgc gggagttgag cacgctcgct cgttgggcat cccggttatt
    43081 gttaccgatc accatttgcc gggcgacaca ttacccgcag cggaagcgat cattaaccct
    43141 aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat
    43201 ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga gcgcggcatc
    43261 gcaattccta acctggcaga actgctggat ctggtagcac tggggacagt agcggacgtc
    43321 gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg catccgtgcc
    43381 ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg caaatcgcga tgcacaaaaa
    43441 ctagccgcca gcgatttagg ttttgcgctg ggaccgcgtc tcaatgctgc cgggcgactg
    43501 gatgatatgt ccgttggtgt ggcgctgttg ctgtgcgaca acatcggcga agcgcgcgtg
    43561 ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag agatcgaaca aggaatgcag
    43621 gttgaagccc tgaccctgtg cgagaaactg gagcgcagcc gtgacacgct acccggcggg
    43681 ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc ttcgcgtatc
    43741 aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgacgg tacgctgaaa
    43801 ggttccggtc gctccattca ggggctgcat atgcgtgatg cgctggagcg attagacaca
    43861 ctctaccccg gcatgatgct caagtttggc ggtcatgcga tggcggcggg tttgtcgctg
    43921 gaagaggata aattcgaact ctttcaacaa cggtttggcg aactggttac tgagtggctg
    43981 gccccttcgc tattgcaagg cgaagtggtg tcagacggtc cgttaagccc ggccgaaatg
    44041 accatggaag tggcgcagct gctgcgcgat gctggcccgt gggggcagat gttcccggag
    44101 ccgctgtttg acggtcattt ccgtctgctg caacagcggc tggtgggcga acgtcatttg
    44161 aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg atggtattgc ttttaatgtc
    44221 gataccgctc tctggccgga taacggcgtg cgcgaagtgc aactggctta taagctcgat
    44281 atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat ctggccaatt
    44341 tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct cttatcaccg
    44401 cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc ggtaaataat
    44461 cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg actacgacgc
    44521 caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg tctggaacga
    44581 acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac
    44641 cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac tggctgtaga
    44701 agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa
    44761 actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg ccgactgtta
    44821 cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga gcatgcttga
    44881 gcgtatgtat ctgcgctggg cagaatcgcg tggtttcaaa actgaaatca tcgaagagtc
    44941 ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg attacgctta
    45001 cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaaaagcc cgtttgactc
    45061 cggcggtcgt cgccacacgt cgttcagctc tgcgtttgtt tatccggaag ttgatgatga
    45121 tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg cgtccggcgc
    45181 gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca tcccgaccgg
    45241 gatcgtgacc cagtgccaga acgaccgttc ccagcacaag aacaaagatc aggccatgaa
    45301 gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgccg agaaacaggc
    45361 gatggaagat aacaaatctg acatcggctg gggcagccag attcgttctt atgtccttga
    45421 tgactcccgc attaaagatc tgcgcaccgg ggtagaaacc cgcaacacgc aggccgtgct
    45481 ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa
    45541 catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca atgaactgaa
    45601 aacgcgtcgt gagaagctgg cgaacctgcg tgagcaggga attgccttcc cgaacgattt
    45661 ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag agaacgaaga
    45721 actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc gccgtattat
    45781 gggtaaagcg tctttcgtta ccctgcagga tgtgggtggc cgcattcagc tgtacgttgc
    45841 ccgtgacgat ctgccggaag gcgtttacaa cgagcagttc aaaaaatggg acctcggcga
    45901 catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt ctatccactg
    45961 taccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggataaat tccacggctt
    46021 gcaggatcag gaagcgcgct atcgtcagcg ttatctggat ctcatctcta acgatgaatc
    46081 ccgtaacacc tttaaagtac gctcgcagat cctctctggt attcgccagt tcatggtgaa
    46141 ccgcggcttt atggaagttg aaacgccgat gatgcaggtg atcccaggcg gtgccgctgc
    46201 gcgtccgttt atcacccacc ataacgcgct ggatctcgac atgtacctgc gtatcgcgcc
    46261 ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg
    46321 taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca tgatggaact
    46381 ctacatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc tgttccgtac
    46441 tctggcacag gatattctcg gtaagacgga agtgacctac ggcgacgtga cgctggactt
    46501 cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac
    46561 cgacatggcg gatctggata acttcgactc tgcgaaagcg attgctgaat ctatcggtat
    46621 ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg aagaagtggc
    46681 agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag tttctccgct
    46741 ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct tcatcggtgg
    46801 tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg cgcaacgctt
    46861 cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct acgatgaaga
    46921 ttacgttacc gcactggaac atggcttacc gccgacagca ggtctgggga ttggcatcga
    46981 ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc tgttcccggc
    47041 gatgcgtccg gtgaaataag cattacgtta tgctcacaac cccggcaaat gtcggggttt
    47101 tttatttaag ctgggtaaat gcagataatc gtattctggc ttcgcgattt gtcgcctgca
    47161 tcaccatcca cggactgaac gcccacggcg tggcatcaat accgcgtaat acatcagcta
    47221 aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca ctggtggtgc
    47281 gtgcggcaaa taccggacac acttcatttt ccacaatgcc tctcggatcg gtggcgcggt
    47341 agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc tcataacggc
    47401 aacggcggat caccgcgtct tcgctgcttt ctcccagttg tgggtgccca caaaccgagt
    47461 tagtccacac gccaggccac gcttttttgc tcagtgcgcg gcgggtaact aataattgcc
    47521 ccttggcatt aaacagccaa ctggagaacg cgagatgtaa gagggtgtct gccgtgtgtg
    47581 cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt
    47641 ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat aattgaagag
    47701 tgaaatgatt aaccaagaga gcgtttatcg acaaccgccc gttttgagcg gatgccgaca
    47761 ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa tggccattgc
    47821 aacacagtca gaaatgacag gaatagagag agtaaaaagc aacaacagac tgaatatagc
    47881 gcgcattaaa aaattcatat tattctccat gctcattttt cttcagcttg cgcatcagaa
    47941 gccacatacg tactgtcctg acttctaaat ctttatcaga gacggattcc ttcaccaggt
    48001 cagcatgatg ctgatagcca ttaaagaaga cgttcagcac aacggcgcta agggttgcga
    48061 gcataatgcc gctatgtagc agcggttgca gtacggccgg taacttagaa aagaaatcgt
    48121 gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag aggttgtaac
    48181 ggttggtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt accatgccga
    48241 acatcactag accagcgccg cccagcacaa attgcggaat ggaggctacc agcaccgcca
    48301 tttttggcac catgccaaac aggattaata taattcccga agaaatacac acccagcggc
    48361 tatgaacgcg catcacgcta accaggccaa cgttttgaga aaatgacgtg tgggggaagc
    48421 tgttaaacgt gccgcctatc attgtcccta cgccatcgac acgcagcccg caaataatat
    48481 cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc cccatcgact
    48541 cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga tcgaaaatcg
    48601 gcataccaaa cgacatcggg gtgacaatcg caaaccacga ggcatcatgt agcccggata
    48661 aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc agtaatacgg
    48721 cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa attaagacgg
    48781 caaaggagat acctaaataa acgggattac catattgcgg attcccttta cctccagcgg
    48841 cccagtcaat acccacctga atgatgctaa gcccaataga agtaataacc acaccggtaa
    48901 ccagtggcgg gaataaaggc atcaagcgac caataagtgg cgctaataat gtggtgataa
    48961 aacccgcggc gatagtggca ccaaatatcc ccagcaggcc gatatccggg ttcatcccaa
    49021 tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc aggcggatcc
    49081 ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag caaaagagat
    49141 ccgagctaat gagcatcgca atagcatctt ttgagaggcc cagtcggtca ccaatcataa
    49201 gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg agtatgatca
    49261 gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc ccagaagatg
    49321 agggaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc cagaataaaa
    49381 agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc cagtttttgc
    49441 gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg cttaaaccac
    49501 gaaaatgaca agagaaactg acaatggaat tatctgtaat tttctcatat tgatatttca
    49561 ttaatcattc tggcatgtcg cactctcgca tttaatcgtt tttatctgga tagcgctctt
    49621 ttgatcggca atgttgaaat aatgaaagcg gcatagattg aggggcaata atttgaactc
    49681 agtcgcaaaa ttataattaa aacggtcatg ctacctaata tatgcccgtt tattgcatcg
    49741 cgaaatttga gaagacataa ctcattaaat gaaagggatg caaatgaata cgtttatcgc
    49801 tgctgaagct gcggaatgta
//
LOCUS       DAJTUI010000020        49648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTUI010000020 DAJTUI010000000
VERSION     DAJTUI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.020"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..99
                     /locus_tag="OO464_001255"
     CDS             <1..99
                     /locus_tag="OO464_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6226473.1"
                     /translation="LARLGRKSLSFSKSVELYDKVIGHYLNIKHYQ"
     gene            complement(93..1271)
                     /gene="setB"
                     /locus_tag="OO464_001256"
     CDS             complement(93..1271)
                     /gene="setB"
                     /locus_tag="OO464_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR6226474.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFWVMTPTY
                     "
     gene            1639..2769
                     /gene="fruB"
                     /locus_tag="OO464_001257"
     CDS             1639..2769
                     /gene="fruB"
                     /locus_tag="OO464_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6226475.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2769..3707
                     /gene="fruK"
                     /locus_tag="OO464_001258"
     CDS             2769..3707
                     /gene="fruK"
                     /locus_tag="OO464_001258"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6226476.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3724..5415
                     /gene="fruA"
                     /locus_tag="OO464_001259"
     CDS             3724..5415
                     /gene="fruA"
                     /locus_tag="OO464_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6226477.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5839..6780
                     /locus_tag="OO464_001260"
     CDS             5839..6780
                     /locus_tag="OO464_001260"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6226478.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6768..7706
                     /gene="psuG"
                     /locus_tag="OO464_001261"
     CDS             6768..7706
                     /gene="psuG"
                     /locus_tag="OO464_001261"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6226479.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7800..9050
                     /locus_tag="OO464_001262"
     CDS             7800..9050
                     /locus_tag="OO464_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6226480.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9121..9815)
                     /gene="yeiL"
                     /locus_tag="OO464_001263"
                     /pseudo
     CDS             complement(9121..9815)
                     /gene="yeiL"
                     /locus_tag="OO464_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9946..10887
                     /gene="rihB"
                     /locus_tag="OO464_001264"
     CDS             9946..10887
                     /gene="rihB"
                     /locus_tag="OO464_001264"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR6226481.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10987..12237
                     /locus_tag="OO464_001265"
     CDS             10987..12237
                     /locus_tag="OO464_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6226482.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12293..13381)
                     /locus_tag="OO464_001266"
     CDS             complement(12293..13381)
                     /locus_tag="OO464_001266"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6226483.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13384..14241)
                     /gene="nfo"
                     /locus_tag="OO464_001267"
     CDS             complement(13384..14241)
                     /gene="nfo"
                     /locus_tag="OO464_001267"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR6226484.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14315..15364)
                     /locus_tag="OO464_001268"
     CDS             complement(14315..15364)
                     /locus_tag="OO464_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6226485.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15463..16344
                     /locus_tag="OO464_001269"
     CDS             15463..16344
                     /locus_tag="OO464_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6226486.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16550..18019
                     /gene="lysP"
                     /locus_tag="OO464_001270"
     CDS             16550..18019
                     /gene="lysP"
                     /locus_tag="OO464_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6226487.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18311..20302
                     /gene="cirA"
                     /locus_tag="OO464_001271"
                     /pseudo
     CDS             18311..20302
                     /gene="cirA"
                     /locus_tag="OO464_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20334..21169)
                     /gene="yeiG"
                     /locus_tag="OO464_001272"
                     /pseudo
     CDS             complement(20334..21169)
                     /gene="yeiG"
                     /locus_tag="OO464_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21427..22095
                     /gene="folE"
                     /locus_tag="OO464_001273"
     CDS             21427..22095
                     /gene="folE"
                     /locus_tag="OO464_001273"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6226488.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            22112..23188
                     /locus_tag="OO464_001274"
     CDS             22112..23188
                     /locus_tag="OO464_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6226489.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23330..24370
                     /gene="galS"
                     /locus_tag="OO464_001275"
     CDS             23330..24370
                     /gene="galS"
                     /locus_tag="OO464_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR6226490.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24650..25648
                     /gene="mglB"
                     /locus_tag="OO464_001276"
     CDS             24650..25648
                     /gene="mglB"
                     /locus_tag="OO464_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6226491.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25709..27229
                     /gene="mglA"
                     /locus_tag="OO464_001277"
     CDS             25709..27229
                     /gene="mglA"
                     /locus_tag="OO464_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6226492.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27245..28255
                     /gene="mglC"
                     /locus_tag="OO464_001278"
     CDS             27245..28255
                     /gene="mglC"
                     /locus_tag="OO464_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6226493.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28291..29504)
                     /gene="preA"
                     /locus_tag="OO464_001279"
                     /pseudo
     CDS             complement(28291..29504)
                     /gene="preA"
                     /locus_tag="OO464_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29498..30736)
                     /gene="preT"
                     /locus_tag="OO464_001280"
     CDS             complement(29498..30736)
                     /gene="preT"
                     /locus_tag="OO464_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR6226494.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30930..31169)
                     /locus_tag="OO464_001281"
     CDS             complement(30930..31169)
                     /locus_tag="OO464_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6226495.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31172..31891)
                     /gene="sanA"
                     /locus_tag="OO464_001282"
     CDS             complement(31172..31891)
                     /gene="sanA"
                     /locus_tag="OO464_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6226496.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(32041..32925)
                     /gene="cdd"
                     /locus_tag="OO464_001283"
     CDS             complement(32041..32925)
                     /gene="cdd"
                     /locus_tag="OO464_001283"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6226497.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33055..33750)
                     /locus_tag="OO464_001284"
     CDS             complement(33055..33750)
                     /locus_tag="OO464_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6226498.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33747..34145)
                     /locus_tag="OO464_001285"
     CDS             complement(33747..34145)
                     /locus_tag="OO464_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6226499.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34384..35331
                     /gene="dusC"
                     /locus_tag="OO464_001286"
     CDS             34384..35331
                     /gene="dusC"
                     /locus_tag="OO464_001286"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6226500.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35702..35785)
                     /gene="yohP"
                     /locus_tag="OO464_001287"
     CDS             complement(35702..35785)
                     /gene="yohP"
                     /locus_tag="OO464_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR6226501.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            36009..37445
                     /gene="mdtQ"
                     /locus_tag="OO464_001288"
                     /pseudo
     CDS             36009..37445
                     /gene="mdtQ"
                     /locus_tag="OO464_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37498..38259
                     /locus_tag="OO464_001289"
                     /pseudo
     CDS             37498..38259
                     /locus_tag="OO464_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38376..38954)
                     /locus_tag="OO464_001290"
     CDS             complement(38376..38954)
                     /locus_tag="OO464_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6226502.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39124..39711
                     /locus_tag="OO464_001291"
     CDS             39124..39711
                     /locus_tag="OO464_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6226503.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39885..40817
                     /gene="pbpG"
                     /locus_tag="OO464_001292"
     CDS             39885..40817
                     /gene="pbpG"
                     /locus_tag="OO464_001292"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6226504.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40855..42570)
                     /gene="dld"
                     /locus_tag="OO464_001293"
     CDS             complement(40855..42570)
                     /gene="dld"
                     /locus_tag="OO464_001293"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6226505.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42766..45063
                     /gene="bglX"
                     /locus_tag="OO464_001294"
     CDS             42766..45063
                     /gene="bglX"
                     /locus_tag="OO464_001294"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6226506.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45315..46232
                     /gene="osmF"
                     /locus_tag="OO464_001295"
     CDS             45315..46232
                     /gene="osmF"
                     /locus_tag="OO464_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR6226507.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46239..47396
                     /gene="yehY"
                     /locus_tag="OO464_001296"
     CDS             46239..47396
                     /gene="yehY"
                     /locus_tag="OO464_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR6226508.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47389..48315
                     /gene="yehX"
                     /locus_tag="OO464_001297"
     CDS             47389..48315
                     /gene="yehX"
                     /locus_tag="OO464_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR6226509.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48320..49051
                     /gene="yehW"
                     /locus_tag="OO464_001298"
     CDS             48320..49051
                     /gene="yehW"
                     /locus_tag="OO464_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6226510.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(49032..49139)
                     /locus_tag="OO464_001299"
     CDS             complement(49032..49139)
                     /locus_tag="OO464_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR6226511.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49199..49648)
                     /locus_tag="OO464_001300"
                     /pseudo
     CDS             complement(49199..49648)
                     /locus_tag="OO464_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11993 a        12227 c        13340 g        12088 t
ORIGIN      
        1 ctggcaaggc tgggacggaa gtcactgtcg ttctcaaaat cggtggagct gtatgacaag
       61 gtcatcgggc attatctgaa cataaaacac tatcagtaag ttggagtcat tacccaaaac
      121 agagtggcga taatcatcac catcgcaaac cagaacacag cgtgataatt ccagatctcg
      181 gcgacgatgc ccgccactga tcctgcgatg atccagccca cgcgcgacgt gttggtatag
      241 agcgtggtgg ctgaacctgc ctgaccgggc atcaaatcct gaaaatagag catcccgatg
      301 ccgcccagaa tgccaataaa aatagcattt agcagctgca agcccaacag aatggcaggt
      361 gaatgcgcca tcagcattcc tgcgtaaaaa cagacgccac ccacggcagc aacgcgcatt
      421 aagaaacgct tacccagacg tttggcgaaa tatccggcaa tcaacatcgt cgggatttcc
      481 agcccggcgg cggtccccat catcacaccg gccagtttct cgggaagatg cagttcgttg
      541 ataataaata gcggcatgtt gatgatgtac aggctgttcg agccccacat caatgtacaa
      601 atgacaaaca gcagtagcgt atcgcgacgg ttacggcgcg gcgcttcaac cgtgccggtc
      661 gccagcggaa gctctttttg catcgacggt aaaaacagcc acaccatcac gccgcaaaca
      721 ataaacgcta ccgctgcgct cagatacatt accgtaaagc tgaaacccat cgctaacgca
      781 taagccagcg gtgggccaat gacccatgcc agtgaaacct gagcgcgtaa aaaagagctg
      841 aacatcaccg cctcacgtcc ggttttgtcg gcatgttcac gggcaagggc aaacatttgc
      901 gggttagcgg tcgagccaaa gctgctaaga aagacgccaa cgaatagcaa aacaaagtag
      961 ttgcgattcc aagcaaaaag ggtgcaggcc agcacgccta acaggcagca aaagacaatc
     1021 agcgatttgc gatcgccgcg cttatcagag cgcccagcga gaaactgact taccagaatc
     1081 ccaatgacag cgctgccggt aaagaagaat cccaccatcg ccggacgggc atgtacttca
     1141 tcggtaagga aaatactgag tgtcggggtt tgcagagcac ccgcaatacc ggtgagaaag
     1201 gcaacgatta aaaacgccgt cgaggtcagg tcaaacgatt tcgcgctgga gactgcgggg
     1261 gagttatgca tgctggtcga tcactggaag aggaagacgc ggagtttacg ctgcttgtca
     1321 gaaaataaac agacagcgaa atcaaaagcg ccacgaataa tcaaaatgcc atttcactgt
     1381 tgctgattgg ctgctgacgc tgctgaaatg aaattgctga taacggattt tcccatcagc
     1441 aattaggaaa aatgacaaaa aattgtgcag cacatcaaac ttttgctcat aactttacgg
     1501 ctttccttgc gtgctgaaac agaagtatta tgctttcttg aaacgtttca gcgcgatctt
     1561 gtctttaacc ctaagctagg ttggcgcttt ttttctcata gaggctgaat cgtttcaatt
     1621 cagcaagaga ggagaactat gttccagtta tccgtacagg acatccatcc gggcgaaaag
     1681 gccggagaca aagaagaggc gattcgccag gtcgctgcgg cgctggtgca ggccggtaat
     1741 gtagcagaag gctacgtcaa tggcatgctg gcgcgcgaac agcaaacctc aacgttcctc
     1801 ggcaatggta ttgctattcc acacggcact accgacaccc gcgatcaggt gctgaaaacc
     1861 ggcgttcagg tatttcagtt cccggaaggc gtcacctggg gtgacggtca ggtagcgtac
     1921 gtggcaatcg gtattgctgc cagctcggat aaacatctgg gcctgctacg ccagctgacc
     1981 cacgtactga gcgatgattc cgttgctgaa caactgaagt cagcaacaac agcagaagaa
     2041 cttcgcgcat tactgatggg cgaaaagcag agtgagcagc tgaagctcga caacgaaatg
     2101 ctgacgctgg atatcgtcgc cagcgatctg ctgactcttc aggcgctgaa cgctgcgcgt
     2161 ctgaaagagg cgagggcagt tgacgccact ttcgtcacca aagccatcaa tgaacaaccg
     2221 ctgaatctcg gacagggtat ctggctgagc gatagcgccg aaggcaatct gcgtagtgcg
     2281 attgcggtaa gccgtgcggc aaatgctttt gatgtggacg gcgaaacggc agccatgctg
     2341 gtgagtgtgg cgatgaatga cgatcagccc atcgcggttc ttaagcgtct cgctgatttg
     2401 ttgctcgaca ataaagctga ccgcttgctg aaagcggatg cggcaacgtt gctggcgctg
     2461 ctgaccagcg atgatgcgcc gaccgacgac gtgttaagcg cggagtttgt ggtgcgcaat
     2521 gaacacggcc tgcatgctcg tccaggtacc atgctggtca ataccattaa acaatttaac
     2581 agtgatatta ccgtgacaaa ccttgatggc accggcaaac cggcaaacgg acgtagtctg
     2641 atgaaagttg tggcacttgg cgttaagaaa ggtcatcgcc tacgctttac cgcccagggt
     2701 gcagatgctg aacaggcgct gaaagccatt ggcgacgcta tcgctgctgg tcttggggag
     2761 ggcgcataat gagcagacgt gttgctacga tcacccttaa tccggcttat gaccttgttg
     2821 gtttctgccc ggaaattgaa cgcggcgaag tgaacctggt gaaaaccacc ggtctgcatg
     2881 cggcgggtaa aggcatcaac gtggccaaag tattaaaaga cctgggaatt gatgtcaccg
     2941 ttggcggctt cctgggtaaa gacaatcagg atggttttca gcaactgttc agcgagctgg
     3001 gcattgccaa ccgtttccag gttgtacagg ggcgcacccg aattaacgtt aagctgacgg
     3061 aaaaagacgg cgaagtgacc gacttcaact tctcgggttt cgaagtcacc cccgccgact
     3121 gggaacgctt tgtgactgat tctctgagct ggctcggtca gttcgatatg gtctgtgtca
     3181 gcggaagctt accgtcaggc gtcagcccgg aagcgttcac cgactggatg actcgcctgc
     3241 gtagtcagtg tccttgcatt atctttgata gtagccgtga agcgttagta gcaggtttga
     3301 aagcggcacc gtggctggtg aaacctaacc gccgcgagtt ggaaatctgg gcaggccgta
     3361 aactgcctga aatgaaagat gtgattgaag ctgcacatgc gctacgtgaa caaggtattg
     3421 cgcatgttgt tatttcactg ggtgccgaag gcgcgctttg ggttaatgcc tccggcgaat
     3481 ggatcgctaa accaccgtca gtcgatgtcg taagcaccgt tggcgcaggg gattctatgg
     3541 ttggtggcct gatttatggc ttgctgatgc gtgaatccag tgaacacaca ctgcgtctgg
     3601 cgacagctgt tgcagccctg gcggtaagtc aaagcaatgt gggtattacc gatcgtccgc
     3661 agttggccgc aatgatggcg cgcgtcgact tacaaccttt taactgacag caggagaggc
     3721 ataatgaaaa cgctgctgat tattgacgct aatctcggtc aggcacgcgc ctatatggcg
     3781 aagaccctgc tgggcgcggc ggcgcgaaaa gcaaaactgg aaatcatcga caatccgaac
     3841 gacgcagaaa tggcgattgt tctcggtgat tccatcccga atgacagcgc gctgaacggt
     3901 aaaaatgtct ggctgggtga tatttcccgg gcagttgcgc accctgagct gttcctgagt
     3961 gaagccaaag gccatgcgaa accttacact gcgccggtca ctgcgacagc accggttgcc
     4021 gccagcggtc cgaaacgcgt agttgcggtg actgcttgcc cgactggcgt agcacacacc
     4081 tttatggcgg ctgaagccat tgaaaccgaa gcgaaaaaac gtggctggtg ggtgaaagtt
     4141 gaaacccgtg gttctgttgg cgcgggtaat gcaatcactc ccgaggaagt agccgcagcg
     4201 gatctggtga ttgtggcggc agatatcgaa gtggatctgg cgaaatttgc tggtaaaccg
     4261 atgtatcgta cctctaccgg tctggcgctg aagaaaacct cgcaggaact ggataaagcg
     4321 gttgctgaag caacgccgta tgaaccggcg ggcaaagctc aaacggcgac cactgaaggt
     4381 aagaaagaga gtgcaggcgc ttatcgtcac ttgctaacgg gcgtctctta catgctgccg
     4441 atggtcgttg ctggtggtct gtgtatcgcg ctttcttttg cttttggtat cgaagcgttt
     4501 aaagagccgg gtacgttggc agcggcgctg atgcagattg gtggtggttc agcctttgcg
     4561 ctgatggtgc cggtactggc aggttatatt gccttctcca ttgccgatcg tccgggcctc
     4621 acgccgggtc tgattggcgg tatgctggcg gtcagcaccg gttctggctt cattggcggt
     4681 attattgcgg gcttcctggc tggttacatt gcgaagttaa tcagtacgca actgaaactg
     4741 ccgcagagta tggaggcgct gaaaccgatc ctgatcattc cgctaatttc cagtctggtg
     4801 gtcggtctgg cgatgatcta cctgatcggt aaaccagttg ctggcattct cgaagggctg
     4861 actcactggc tgcagaccat ggggactgcg aatgcggttc tgctgggggc gatcctcggt
     4921 ggcatgatgt gtactgacat gggcggtccg gtaaacaaag cagcgtacgc attcggtgtg
     4981 ggtctgctga gtactcaaac ctatggcccg atggcggcga ttatggcggc aggtatggtg
     5041 ccaccgctgg caatgggcct ggcaacaatg gtggcgcgtc gcaaattcga caaagcgcag
     5101 caggaaggtg gcaaagccgc tctggtattg ggactgtgct tcatttcgga aggtgcaatt
     5161 ccgtttgctg ctcgtgatcc gatgcgtgtg ctgccgtgct gtatcgtggg tggggcgctg
     5221 actggcgcaa tctcaatggc gattggtgcg aaactgatgg caccgcacgg tggtctgttt
     5281 gttctgctga tccctggcgc tattacgccg gtattgggtt acctggtagc aattattgcc
     5341 ggtacgctgg tggcgggttt ggcctatgcc ttcctgaaac gtccggaagt ggacgcagta
     5401 gcgaaagcag cgtaataaaa ggtgtgttac agggcagaaa tttcctgccc tgtaacattt
     5461 gatgagcgca aagagaatga ccagcttgtg aatgtaattt tatgaattta aatggtttta
     5521 tctttcagta gaaacttcaa acgttcatca aacactaagg ttatttcctc aagggcagat
     5581 tattttctgc ccttttttag tgttcctgtt ctgtgcatcc cgtcacaaat tcctcgcttt
     5641 tgtaattcag gcatttaatc gcattttgtg agcctgctca aatctgccca ttttgcctct
     5701 ctggcggtaa tttactttac gtaaaacatg catactatga gcacatgttt aaatatgaac
     5761 acatgtttaa accgtctttg cagtatctat attcttgtgg attgacaggg ttggtaattt
     5821 tttaccagag gacgtgctat gcgcgaaaag gattatgtcg taattatagg ttcggcgaat
     5881 attgatgtcg ccggatattc acatgaatca ttaaattatg cggattcaaa tccaggtaaa
     5941 ataaaattta cgcctggtgg agtagggcgc aatattgcac aaaacctggc gttgctgggt
     6001 aacaaagcct ggctactgag cgccgtaggc agtgattttt atggtcaatc gctgctaacg
     6061 caaaccaatc aatctggtgt ttatgtcgat aaatgcctga ttgtgccggg agaaaatacg
     6121 tcgagttatt tatcattact cgataatacc ggtgaaatgc tggttgctat aaatgacatg
     6181 aatattagca acgctattac agctgaatat ctcgcacagc accgtgaatt tattcagagg
     6241 gcaaaggtca ttgtcgcgga ctgtaatatc agtgaagagg cactggcatg gattctggat
     6301 aatgccgcca acgtacccgt atttgtcgat ccggtttcag cgtggaaatg tgtcaaagtg
     6361 cgcgaccgtc taaatcagaa ccacactctg aagccaaacc gccttgaagc ggaaaccctg
     6421 agtgggattg cgctgtcagg gcgtgacgat gtggcaaaag ttgctgcctg gttccatcaa
     6481 catggcctga accgactggt attgagcatg ggcggcgacg gcgtttatta cagcgatatc
     6541 agcggtgaaa atggctggtc tgcgccgatc aaaaccaatg ttattaatgt taccggagcg
     6601 ggcgatgcca tgatggcggg acttgcttcg tgttgggtag acggaatgcc gtttgccgaa
     6661 tctgttcgtt tcgcacaggg atgttcgtca atggcgctct cctgtgaata caccaataac
     6721 cccgatttat cgattgccaa cgttatatcg ttagtggaga acgcagaatg tctgaattaa
     6781 aaatttcccc tgaattatta caaatttctc cggaagtgca ggacgcttta aaaaacaaaa
     6841 aaccggttgt ggcgctggaa tcgaccatta tttctcacgg gatgccgttc ccacaaaatg
     6901 cccagaccgc aattgaagta gaagaaacta ttcgtaaaca gggcgcagta cctgccacta
     6961 tcgccattat tggcggcgtg atgaaagtgg gtttaagcaa agaaaaaatt gaattactgg
     7021 gtcgtgaagg gcataacgtg accaaagtta gtcgtcgcga tttacctttt gttgttgccg
     7081 ccggaaaaaa tggcgcaacc actgtggctt caacgatgat tattgcggcg cttgccggaa
     7141 ttaaagtatt tgccaccggg ggaattggtg gtgtgcatcg cggggcggaa cataccttcg
     7201 atatttctgc cgatttgcaa gaactggcaa atactaatgt caccgttgtt tgtgccgggg
     7261 cgaaatctat tctcgattta ggattaacca ctgagtattt agaaaccttc ggtgtgccgt
     7321 taattggcta tcagactaaa gcgctgcctg cgtttttctg ccgtaccagc tcgtttgacg
     7381 tcagcattcg tctcgacagc gccagtgaaa ttgcccgtgc aatggcggtg aaatggcaaa
     7441 gcgggctgaa cggtggcctc gtggtagcga acccgatccc ggaacagttt gcgatgccgg
     7501 aggaatctat caatgcagcc atagatcaag ccgtcgccga agccgaagag cagggcgtta
     7561 ttggtaaaga aagtacaccg ttcctgctgg cgcgcgttgc tgagctgacc agcggtgaca
     7621 gcctgaaatc caatatccag ctggtgttca acaacgccat tctggcgagt gaaattgcca
     7681 aagaatacca gcgtctcgcg ggttaatttc tgacgggcag aaagccgtcg cccgtactca
     7741 tcaagcatac caaaacctgg cgctgacgcc gggtttactg gcatgaaggg aaaatcatta
     7801 tggatataat gagaagtgtt gtggggatgg tggtgttact ggcaatagca tttctgttgt
     7861 cagtgaataa aaagagcatc agtttgcgca cggttggagc cgcactgctg ctgcaaatcg
     7921 ctattggtgg catcatgctc tacttcccac cgggaaaatg ggcagtagaa caggcggcat
     7981 taggcgttca taaagtgatg tcttacagtg atgccggtag cgccttcatt tttggttcgc
     8041 tggttgggcc gaaaatggat gtcctgtttg acggtgcggg ttttatcttc gcctttcgcg
     8101 tacttccggc gattattttc gttactgcgc tcatcagtct gctgtactac attggcgtga
     8161 tggggctgct gattcgcatc cttggcagca ttttccagaa agccctcaac atcagcaaaa
     8221 tcgaatcttt tgttgcggtt accactattt tcctcgggca aaatgagatc ccggcgatcg
     8281 ttaaaccgtt tatcgatcgc gtgaatcgca acgagttgtt taccgcaatt tgtagcggga
     8341 tggcgtccat tgctggttcg gtgatgattg gttatgccgg aatgggcgta ccaattgact
     8401 acctgttagc ggcatcgctg atggcgatcc ccggcggtat tttgtttgca cgtattctta
     8461 gcccggcaac cgagccttcg caggtcacat ttgaaaatct gtcgttcagc gaaacgccgc
     8521 caaaaagctt tatcgaagcg gcggcgagcg gtgcgatgac cgggctgaaa atcgccgctg
     8581 gtgtagcgac ggtggtaatg gcgtttgtcg caattattgc gctgatcaac ggcattatcg
     8641 gcggaattgg cggctggttt ggtttcgcca atgcctctct ggaaagtatt tttggctatg
     8701 tgctggcacc gctggcgtgg atcatgggtg tggactggag tgatgccaac cttgctggta
     8761 gcctgattgg gcagaaactg gcgattaacg aattcgtcgc ttacctgagt ttctccccat
     8821 acctgcaaac gggcggcacg ctggaagtga aaaccgtggc gattatatcc tttgcgcttt
     8881 gtggttttgc taactttggt tctatcggtg ttgtcgttgg cgcattttcg gctatttcgc
     8941 caaaacgtgc tccagaaatc gcccagcttg gtttacgggc gctggcagca gcaacgcttt
     9001 ccaacctgat gagtgcgact attgccgggt tctttattgg tctggcgtaa tgctcgattt
     9061 gtagaccgga taaaacgtat taagcgttgc atccggcaat cgcgctattg caaataattt
     9121 ttactgcatc atcccggaga atttattctc cgggtccatc tccagcgcca gtcctgacaa
     9181 ctgctttctg tttttaatta gatatccttt cttttaatta ataaaccgtc gtgaatgaat
     9241 tgcgcgagaa catataaaag atgtcgataa gaaacgccta aatactctgc cgcttgcgta
     9301 tgcttttcgt gataaagatc accttcttgt gagagtaata taaatgctgc caggcgattc
     9361 actaacggaa atgattgatt ctgagttaaa gaaacaatat tacgataatt tttatgactt
     9421 aaggtgacgc aaagttttcg taaaaatagg gtgtcgttta ataacagcgg acggtaatgt
     9481 ttcataggga gcgcaaggca ccaacactct tcaatagcct gcaccgcacg cggttcatgg
     9541 tctttgtcga ttaactcaat ctcgccaata aaacagggag cggcaaagaa atcgatcagc
     9601 gacacgcgac cattggcaag cgtggcgtaa agcctggcgc ggcctcgcgt cagataaaac
     9661 agccatgacg gctgttgccc ttcctgcaca atgtagtcac gcgctaaaaa atgaaacagc
     9721 cgcgtatcgg ctgaaacatc cgttgaaaag caatccttaa acgcagattc actcatcaat
     9781 tgctgcttaa ggtcattatt gtggatttct ttcacgatgt ttatccgcta tgagatttct
     9841 catatttagc atacgtacgc tgttgttaag attcaatcat tggcccggtc gaattttctt
     9901 tgctgtagaa aaaaataacg caactggaaa cagaggaaat aaacaatgga aaagagaaaa
     9961 attattctgg attgtgagcc gggtcatgat gatgctattg caatgatgat ggcagcgaaa
    10021 catccggcaa tagatttatt aggcatcact attgtagcgg gtaatcagac gcttgataaa
    10081 acattaatta atggcctgaa tgtttgccag aaactggaga ttaatgttcc ggtttatgcg
    10141 gggatgccgc aacccattat gcgtaaacaa atcgttgccg ataatattca cggtgaaacc
    10201 ggactggatg gcccggtatt cgagccgctg acccgccagg cagaaagtac ccatgcggtg
    10261 aaatatatca tcgataccct gatggcaagc gatggcgata tcactctggt gccggttggt
    10321 ccgctttcaa atatcgcggt ggcaatgcgt atgcaacccg caatcctgcc caaaatccgt
    10381 gaaattgtgc taatgggcgg cgcttacggt acaggcaact tcaccccatc tgccgagttc
    10441 aatatctttg ccgacccgga agccgcccgc gtggtgttca cctccggcgt tccattagtg
    10501 atgatgggcc tcgatcttac caaccagacc gtttgcaccc cggatgtaat tgctcggatg
    10561 gaaagggtag gcggccctgc cggagagctg ttcagcgaca tcatgaactt cactctcaaa
    10621 acgcagttcg aatactacgg cctggctggc ggcccggtgc atgacgccac ctgcattggt
    10681 tatctgatca accctgatgg cattaaaacc caggatatgt acgtcgaagt ggacgtcaac
    10741 agcggccctt gctacgggcg tacggtctgc gacgaactgg gcgttcttgg caagcccgct
    10801 aataccaaag tcggcatcac tattgatacc gactggttct ggggcttagt cgaagagtgc
    10861 gtgcgcggct acatcaaaac ccattaagtg ttgataacaa gccgggacag tatcgcaaga
    10921 tgcaggcccg gctatttctg atgggcagaa agccgtcgcc caaaatgaca tgaagagata
    10981 atcactatgg atgtcatgag aagtgttctg ggaatggtgg tactgctggc aattgcgttt
    11041 ttactgtcag taaacaagaa gaagatcagc ctgcgtaccg ttggcgcggc gttagtgtta
    11101 caggtcgtga ttggcgggat tatgctttgg ttaccgccag ggcgttgggt cgcagaaaaa
    11161 gtcgcttttg gcgtccacaa agtgatggcg tacagcgacg caggtagcgc atttatcttc
    11221 ggttctctgg tcggaccgaa aatggatacc ttatttgatg gcgcaggatt tatctttggt
    11281 ttcagggtat taccggcaat tatcttcgtc actgcactgg tgagtattct ctactacatc
    11341 ggtgtgatgg ggatcttaat tcgcattctc ggcggtatct tccagaaagc tttaaatatc
    11401 agcaagatcg agtcattcgt cgcggtcacc accattttcc tcgggcaaaa cgaaattccg
    11461 gcgatcgtca aaccctttat cgatcgtctg aatcgcaatg aattatttac agcgatttgt
    11521 agtggcatgg cctcgattgc tggttcgaca atgattggtt acgccgcact gggcgtacct
    11581 gtggaatatt tgctggcggc atcgttaatg gcgatccctg gcgggatctt gtttgcccgc
    11641 ctgttaagcc cggctacgga atcttcgcag gtttctttta ataacctctc tttcaccgaa
    11701 acaccgccaa aaagcattat tgaagccgat gcgacagggg caatgaccgg gctgaaaatc
    11761 gccgccggtg tagcgacagt tgttatggca tttgtcgcca tcattgcgtt aattaacggt
    11821 attatcggcg gcgttggcgg ctggtttggt tttgaacatg cctcgctgga gtccatttta
    11881 ggttacctgc tggccccact tgcgtgggta atgggtgttg actggagtga tgcaaatctt
    11941 gccgggagtt tgattggaca gaaactggca ataaatgaat ttgtcgctta tctcaatttc
    12001 tcaccctatc tgcaaacggc tggcactctg gatgctaaaa ccgtggcgat tatttccttc
    12061 gcgttgtgcg gtttcgctaa ctttggttct atcggggtgg tggtgggggc gttttctgcg
    12121 gttgcgccac accgtgcgcc ggaaatcgcc cagcttggtt tacgcgcgct ggcggcggcg
    12181 acactttcta atctgatgag tgccaccatt gccgggttct ttattggttt agcgtagtgt
    12241 gcaatttatg agccggatta gatgcggtca agcgtcgaat ccggcaacat tgttacacca
    12301 ttggcactaa tgacagcgcg ttatcggcag acagggtagg gttgtttaag ctgccgctgg
    12361 cgcgtgaaat tgccgcgcag gccatcgcaa aacgggcgct gtcacggaaa ttgtttccct
    12421 ccagaaagct atataccaga cccgccataa aaccatcgtc agcaccaaaa ctgtctaccg
    12481 tcgtatgcgc tggcacagtc agcaaaaatt gttcaccatc cttttcgctg caataaactg
    12541 actcatcggg caaataaaca aacagttgct gaacaccttg ctgatgcaat gcattcactg
    12601 cggcattacg gtcagcatcg ctggtgatcg cctgtcccca taaaatttcc agctccggta
    12661 aagtgggttt cagggtgtga atatgcgcca gccagtgttt gattttgccc gctttgaatt
    12721 ctgaaacggt atcgacaaac accgggatct catcagcgag ggtaaagacc cattccagcg
    12781 cctcggctgt caggttgcaa tcagccagta ccacgcccgc atgacgaagt aaatcgcgcg
    12841 acccgttcag tagttgcggt gtcaactgtt ccagcagatg ggtatcgtta attgccagca
    12901 cggtttgatc gtctcgattg gcaattgcca gatacgtcga tgtgctttga ccatgcaaac
    12961 gaacgcagcc ggagacattc acgccggcac ggcgcgtttc ttccaggagc atttcgccat
    13021 aaaagtcatc gccaatcact gaaagcaaat ggacgtcacg gcctaacagc gccagattgt
    13081 gggcgatatt gcgtccaacg ccgcctgccg aacaatgaat tgtaccggga tgagaagccg
    13141 cttgcgggta atggatatcc gccatcccgc gaatatccat attgattgtc cccaccacta
    13201 cgcagtattc ctgctcggtg agaatgtaac ctttgtcttt aatccggcct ttgcgcatta
    13261 aatccataat atgcgccgca acgcgcgaac gactgatttg cagcatgtcc gcaatttcgt
    13321 tctgctgaat cagcgggtta cgccgtaaaa ttgcaaggat ctccttttcc cggttattta
    13381 tcttcaggct accgcttttt cagtttgttg cgctttcagc caggcgatct cttctgccca
    13441 gatatccggg ttgatggttt caaggatcag cggaatgccg tcgaaacggt cgttctgcat
    13501 aatccagcgg aacgcatcat gaccgatatt gccttcaccg aggctatgat ggcggtcaac
    13561 gcggctgcca aaggtacttt tcgcatcgtt aaggtgcatc ccgcgcaaat acttaaagcc
    13621 gacaatacgg gcaaaatcag cgaacgtttt ttcgcattcg gctggagtac gcaaatcata
    13681 cccggcagcg aaagcatggc aggtatcaat gcagacgccg acgcgggatt tatcttccac
    13741 gccgtcgata atcgccgcga gatgttcgaa tttaaaccct aagttactgc cctgaccggc
    13801 ggtgttttca atcaccgctg ttacaccttg agttttatcc agcgcaatgt tgatggattc
    13861 ggcaatacgc gcaaggcaat cctcttctga aatctgcatc agatggctgc cagggtggaa
    13921 gttgagcaaa gaaagcccca gttgttcgca acgctgcatt tcatctataa aggcatcgcg
    13981 cgatttttcc agagcttcag tgaccggatg tccgaggttg atcagataac tgtcgtgggg
    14041 aagaatttgc gccgatgtgt agtgatattt ttcacaggcg gctttgaatt catcgatggt
    14101 ttgcgtcgtg agcggtgcgg cacgccactg acgttggttt ttggtgaaca aggcaaacgc
    14161 ggttgcgtcg atttcggcgg cgcgaattgc ggcatttgcc agaccgccag cagcactaac
    14221 gtgcgctcca atgtatttca tgcgaggact cctgttaaac ccgctggagg aaaacggtaa
    14281 tgatagcggg ttaacaggag caagaagtag tggtttatgc gatgacgctt tgaatcacat
    14341 agttaatcgc accaccacca acaatcagcc aggcaaacag taccagtgcc atcagcagag
    14401 gtttcgcccc agcttttttc agcgcgctga cgtgagtggt cagacccagc gccgccatcg
    14461 ccattgccag caggaaggta tccagcgtta ccagcatgtt caccacgctc tgcggtaaca
    14521 ggtggaacga gttaaagatg gcaactacga tgaacaagat ggcaaaccac ggaatagtga
    14581 ttttgctttt ctcgccgctg ttcgccccag acagctgttt aacacgcgcc gccagcagga
    14641 tgaggaacgg agccagcatc atcacgcgca gcattttgga aataactgct gcgttttctg
    14701 catccgggct gatggcatga cctgccgcca ccacctgcgc cacttcgtgc acagtagaac
    14761 caatgtagat accgaaagtt tccggactaa accattgaga catcagcgga tatatcgccg
    14821 ggtagaggaa aatcgcgacg gtcccgaaga taacaacggt tgcaacagcc acggttactt
    14881 tactggcttc cgctttcact accggctcag tcgccagtac cgcggcagca ccacagatac
    14941 tgctaccggc accgatcaac caactggtgt gcttatccag accaaacact ttctgcccca
    15001 ggaagcaagc cagcaggaag gtactggaca gcgtcagcac gtcaatgatg atcccactga
    15061 taccgacatc ggcaatttgc gagaacgtca gacggaagcc ataaagaatg atacccagac
    15121 gtaataaata ttgcttggca aacagcacac caccgtcaca gcttttccag atgtgcggat
    15181 agatggtgtt gcctaaaacc atccccaaca agattgcgag ggtgagggca ctaaacccgg
    15241 cacccgcaac cgcgggaatg gaaccacccc acagggcgac cccggtgata actgcactca
    15301 gggctaaccc cggaataaaa tgccacagtg tacgatgttg tttctgtaag gtgatattcg
    15361 tcataaccct ctcctttaac cgggcataag gttacgacta actggtttaa aaataaaatt
    15421 gattatatat ttataattaa tctttataag tggtaagcga ctatgcacat caccctccgg
    15481 cagttggaag tttttgcaga agtattgaaa agtggatcaa ccacccaggc gtcggtgatg
    15541 ctggcgttgt cgcaatcagc agtgagcgca gccttgactg acctggaagg gcagcttggc
    15601 gttcaactgt ttgatcgcgt ggggaaaaga ctggttgtta atgaacacgg gcggctgctc
    15661 tatccgcgag cgttggcact gcttgaacag gcggttgaaa tcgaacaact gtttcgcgaa
    15721 gacaacggcg cgattcgtat ctatgccagt agtaccatcg gtaactacat tctgcctgca
    15781 gttatcgccc gttatcgcca tgattatccg cagttgccga ttgaacttag cgttgggaac
    15841 agccaggacg tgatgcaagc ggtgctggat ttccgcgttg atattggctt tattgaagga
    15901 ccgtgccaca gcactgaaat catttctgaa ccgtggctgg aagacgagct ggtggttttc
    15961 gccgcgccga cttcgccgtt ggctcgtggt ccggtcacct tagaacagct ggccgctgcg
    16021 ccgtggatcc tgcgtgaacg cggttccggc acgcgggata ttgtcgatta tctgttgctg
    16081 tcacatttac cgaagtttga gatggcgatg gaattaggta actccgaggc aatcaaacat
    16141 gcggtgcgtc atgggttggg aattagttgc ctgtcgcgac gtgtgattga agatcaattg
    16201 caggcaggca cattaagtga agttgcggtc cctctgccgc gcctgatgcg tacgttgtgg
    16261 cgtatacatc atcggcaaaa acacctttcc aacgcgctac ggcgctttct ggactattgc
    16321 gatcccgcaa atgtgccgcg ttaagttgct gtacaagaac atgctggtgc tgtgtcgatt
    16381 tcgtgacgca gcgccttcag catgcattcg ccagaaaaga gattggctgc tttacttata
    16441 atccctgggc gatcatgaag gtgtcttata accgtgtatt tctgccggaa ggattgccaa
    16501 tcgtctgcta caatcgcgcc atcattttta agatggatag catttttgta tgggttccga
    16561 aactaaaact acagaagcgc cgggcttacg ccgtgaatta aaggcgcgtc acctgacgat
    16621 gattgccatt ggcggttcca tcggtacagg tctttttgtt gcctctggcg caacgatttc
    16681 tcaggcaggt ccgggcgggg cattgctctc gtatatgctg attggcctga tggtttactt
    16741 cctgatgacc agtctcggtg aattggctgc atatatgccg gtttccggtt cgtttgccac
    16801 ttacggtcag aactatgttg aagaaggctt tggcttcgcg ctgggctgga actactggta
    16861 caactgggcg gtgactattg ccgttgacct ggttgcagca cagctggtca tgagctggtg
    16921 gttcccggat acaccgggct ggatctggag tgcgttgttc ctcggcgtta tcttcctgtt
    16981 gaactacatc tctgttcgcg gctttggtga agcggaatac tggttctcac tgatcaaagt
    17041 cacgacagtt attgtcttta tcatcgttgg tgtgctgatg attatcggta tcttcaaagg
    17101 cgcgcagcct gcgggctgga gcaactggac aatcggcgaa gcgccgtttg ctggtggttt
    17161 tgcggcgatg atcggcgtag ctatgattgt cggcttctct ttccagggaa ccgagctgat
    17221 cggtattgct gcaggcgagt ccgaagatcc ggcgaaaaac attccacgcg cggtacgtca
    17281 ggtattctgg cgaatcctgt tgttctatgt gttcgcgatc ctgattatca gcctgattat
    17341 tccgtatacc gatccgagcc tgctgcgtaa cgatgttaaa gacatcagcg tcagtccgtt
    17401 caccctggtg ttccagcacg cgggtctgct ctctgcggcg gcggtgatga acgcagttat
    17461 tctgacggcg gtgctgtcag caggtaactc cggtatgtat gcgtctactc gtatgctgta
    17521 caccctggcg tgtgacggta aagcgccgcg cattttcgct aaattgtcgc gtggtggcgt
    17581 gccgcgtaat gcgctgtatg cgacgacggt gattgccggt ttgtgcttcc tgacctccat
    17641 gtttggcaac cagacggtat acctgtggct gctgaacacc tccgggatga cgggttttat
    17701 cgcctggctg gggattgcca ttagccacta tcgcttccgt cgcggttacg tattgcaggg
    17761 acacgacatt aacgatctgc cgtaccgttc aggtttcttc ccactggggc cgatcttcgc
    17821 attcattctg tgtctgatta tcactttggg ccagaactac gaagcgttcc tgaaagatac
    17881 cattgactgg ggcggcgtag cggcaacgta tattggtatc ccgctgttcc tgattatttg
    17941 gttcggctac aagctgatta aaggaactca cttcgtacgc tacagcgaaa tgaagttccc
    18001 gcagaacgat aagaaataag tttcctccct tccttgctaa gccctctcaa ccgagagggc
    18061 tttttcaatt ccatttccct gacaaatcat gcggatataa aatttaacat ttggattgat
    18121 aattgttatc gtttgcatta tcgttacgac gcaatcaaaa aaggctgaca aatcagaggc
    18181 tgttccggct ttctgggatg atcacctgca tacaaataag tccaccgcga tgctgccgta
    18241 cgcaagggga cgtaaagaag atgtgagcga tacccatttt attttcgtag ttacctcatg
    18301 gagatatgga atgtttaggt tgaacccttt cgtacgggtc gggctgtgtt tgtccgctat
    18361 ttcttgtgca tggcctgtgt tagcggtcga tgatgatggc gaaacgatgg ttgtcactgc
    18421 atcttccgtg gaacaaaatc ttaaagatgc acccgccagt atcagcgtca ttacccagga
    18481 agacctgcag cgaaaaccgg tacagaatct gaaggatgtc ctcaaagaag tgcctggcgt
    18541 acaactgacg aacgaagggg ataaccgtaa gggcgttagt attcgtggtc tggacagcag
    18601 ctacaccctg attcttgtcg acggtaaacg cgttaactcc cgcaatgccg tcttccgcca
    18661 caatgatttc gatctgaact ggatcccggt cgattccatc gaacgtattg aagtggttcg
    18721 tggcccgatg tcgtcgctat acggctccga tgcgctcggc ggtgtagtga atatcatcac
    18781 caaaaaaatc ggtcagaaat ggtcgggtac cgttaccgtc gataccacca ttcaggaaca
    18841 tcgcgatcgc ggtgatacct ataacggtca gttctttacc agtggaccat taattgatgg
    18901 tgtgctggga atgaaagctt acggcagcct ggcaaaacgt gaaaaggatg acccgcaaaa
    18961 ctcaacgacc accgataccg gagaaacgcc gcgtattgaa ggattctcca gccgcgacgg
    19021 caatgtcgaa tttgcctaga caccgaatca aaatcacgat tttactgccg gatacggttt
    19081 cgaccgtcag gatcgtgatt ccgattcgct ggacaaaaac cgcctggaac gccagaacta
    19141 ctgcgtcagc cataatgggc gttgggatta cggcaccagc gaactgaaat cctacggtga
    19201 gaaagtcgag aacaaaaacc ctggcaacag cagcccgata acttccgaaa gcaatacggt
    19261 cgacggcaaa tacacgttgc cgctgacggc gattaatcag tttctcacgg ttggcggtga
    19321 atggcgtcac gacaaactta gcgatgcggt gaacctgacc gggggaacca gctccaaaac
    19381 gtctgccagc cagtacgcgc tgtttgtgga agatgaatgg cggatcttcg agccgctggc
    19441 gctgacgacc ggcgtgcgta tggacgatca cgaaacctac ggtgaacact ggagtccgcg
    19501 tgcctacatg gtttataacg ccaccgacac cgtaacggtg aaagggggct gggcgacggc
    19561 atttaaagca ccttctctgt tgcaacttag ccctgactgg acgagcaatt cctgccgtgg
    19621 cgcatgtaag attgtgggta gcccggatct gaaaccagaa accagcgaaa gttgggagct
    19681 ggggctttac tacatgggtg aagaaggctg gctggaaggg ggtgaatcca gcgttatcgt
    19741 tttccgtaac gatgtgaaag atcgtatcag catcagccgt acgtctgacg tcaacgctgc
    19801 accgggctac caaaactttg ttggttttga gacgggcgct aacggacggc gcataccggt
    19861 atttagctac tacagcgtta acaaagctcg tattcagggc gtggaaaccg aactgaaaat
    19921 tccgttcaac gatgaatgga aactgtcgat caactacacc tacaacgatg gtcgtgatgt
    19981 cagcaacggc gaaaacaaac cgctatccga tctgccgttc catactgcta acggtacgct
    20041 ggactggaaa ccgctggcgc tggaagactg gtcattctat gtttctggtc actataccgg
    20101 gcagaaacgc gccgacagcg cgacggctaa aacaccgggc ggttatacca tctggaatac
    20161 cggcgcggcc tggcaggtga ctaaagacgt caaactgcgc gcaggcgtgc tgaaccttgg
    20221 cgacaaggat ctcagtcgtg acgactacag ctataacgaa gacggacgtc gttactttat
    20281 ggcagtggat tatcgcttct gatgagaaga tgcccggcga accgggcgga ctttcacttc
    20341 agtaaatact gcgcatggaa gcgcaggtga tcctctataa aagaggcgat gaagtagtaa
    20401 ctgtgatcat atcccggctg aatacgcagc gtcatcggcc acgctttctg gcgcgcggct
    20461 tctgccaata ccgcaggttg caactggtcg gcaagaaact gatcattatc gccctgatcg
    20521 ataagcgtcg ggatcgcatc ctgcgcgtta ctggcataca tcagcgcgca actatcccat
    20581 tccagcatgc atttttgtcc tcacctaaat aggtgctaaa cgctttgatt ccccacggga
    20641 cgctgcacgg attcacaatg ggcgcaaagg ccgaaacgct ggtgtattta cccggatttt
    20701 tcagcgccat aatcagcgca ccgtgaccac ccattgagtg accgctaatg gcgcagcggt
    20761 cgctgacatt aaattgcgac tgaaccagcg ccggtaattc atcgcgcaga taatcataca
    20821 tccggtaatg cgtcgcccac ggcggttgcg tggcattaag ataaaagcct gcgccctggc
    20881 ctaaatcgta gccatcgtcg ttggcaacct tttcgccgcg cgggctggtg tctggcatca
    20941 ccagtacaat ccccagttcc gccgctaccc gctgggcacc cgccttggtg gtgaagttct
    21001 cgtcattgca ggttaatccg gaaagccagt acagcactgg tggcggggtg tgatcacgag
    21061 ggggggggag aaagatactg aacgtcatcg ggcagtttaa ggtactggag tcgtgtcgcc
    21121 agcgttgctg ccagccttca aaacagcggt gctcttcgag catttccatg cgtggctcct
    21181 tgttgtgttg tttgcaattt cttcatcata ccgtttattc aatgtgctgt gagtaacttt
    21241 cacttccgta tttgcataac gatgttttaa catctgctga tgaaaggcag cggcaattac
    21301 aataattatc gctgtgaata ctggattatg tgcgccgcct cacgcacaat aatcaagctg
    21361 taaatcagct taataacttt gcccccacgc agggtggagg cgtcacacct gcaggagaaa
    21421 tcataaatgc catcactcag taaagaagcg gccctggttc atgaagcgtt agttgcgaga
    21481 ggactggaaa caccgctgcg cccgcccgtg catgaaatgg ataacgaaac gcgcaaaagc
    21541 cttattgctg gtcatatgac cgaaatcatg cagctgctga atctcgacct ggctgatgac
    21601 agtttgatgg aaacgccgca tcgcatcgct aaaatgtatg tcgatgaaat tttctccggt
    21661 ctggattacg ccaatttccc gaaaatcacc ctcattgaaa acaaaatgaa ggtcgatgaa
    21721 atggtcaccg tgcgcgatat cactctgacc agtacctgtg aacaccattt tgttaccatc
    21781 gatggcaaag cgacggtggc ttatatcccg aaagattcgg tgatcggtct gtcaaaaatt
    21841 aaccgtatcg tgcagttctt tgcccagcgt ccgcaggtgc aggaacgtct gacgcagcaa
    21901 attcttattg cgctacaaac gctgctgggc accaataacg tggctgtctc gatcgacgcg
    21961 gtgcattact gcgtgaaggc gcgtggcatc cgcgatgcaa ccagtgccac gacaacgacc
    22021 tctcttggtg gattgttcaa atccagtcag aatacgcgcc acgagtttct gcgcgctgtg
    22081 cgccatcaca actgattaaa aggcaggaac catggagcgc aacgtcacgc tcgattttgt
    22141 tcgcggcgtc gccattctgg ggatcctgct attaaacatc agcgcctttg ggctaccaaa
    22201 ggcggcttat ctcaatcccg cctggtacga tgctattacg ccgcaggatg catggacctg
    22261 ggcatttctc gatctcatcg gccaggtgaa attcctcacg ctttttgcgc tgctgtttgg
    22321 tgctggcctg caaatgttgc tgccccgtgg cagacgctgg atccagtcgc ggttaacgct
    22381 gttagtcttg ttgggcttta ttcacggttt attgttctgg gacggcgata ttctgctggc
    22441 ttacgggctg gtggggcttg gcgttttgct gttattgggg ctgatttccg acagccagac
    22501 cagccgcgcc tggacgccgg atgcatcggc tattttgtat gaaaaatact ggaagcttca
    22561 cggcggcgtt gaagcgatca gtaatcgtgc cgatggtgtt ggcaacagtt tactggcact
    22621 gggcgcacag tatggctggc aactggcagg gatgatgctc attggtgcgg cgctgatgcg
    22681 cagcggctgg ctgaaagggc agttcagctt acgtcactat cgtcgtactg gttttgtgct
    22741 ggtggcgatt ggggtgacca ttaaccttcc tgccatcgcc ctgcaatggc agctggactg
    22801 ggcgtatcgc tggtgtgcct tcttacttca aatgccgcgg gaactgagtg cgccgtttca
    22861 ggcgattggc tatgcgtcgc tgttttatgg cttctggccg caattgagcc gctttaagct
    22921 ggtgcttgcg atcgcctgcg tcggacggat ggcgctgacc aactatctat tgcaaacgct
    22981 gatttgtacc acgctttttt accacctcgg tttgtttatg cattttgacc gcctggagct
    23041 gctggcgttt gttattccgg tatggctggc gaatatcctc ttctctgtta tctggctgcg
    23101 ttacttccgc caggggccgg tggaatggct ctggcgtcag ttaactttgc gtgctgccgg
    23161 accggcaata tctaaaacat caagataacg atctggatta cattcgttaa caaaacggct
    23221 gtaaccgttt ccattgctgt gactcgattc acgaagtcct gtattcagtg ctgacaaaat
    23281 agccgccagc aagcagtcat ttactgcaat ctcataacag gtagtgaata tgatcaccat
    23341 tcgtgatgta gcgcgtcagg ctggcgtctc tgtggcaacg gtttcccggg tgctcaataa
    23401 cagcacgctg gtcagtgccg acacgcgtga agcagtaatg aaagccgtga gtgagctgga
    23461 ttatcggcca aacgccaatg cccaggcgct ggcaactcag gttagcgaca ccattggcgt
    23521 ggtggtgatg gacgtttctg atgcgttttt cggcgcgctg gtaaaagcgg tggatctggt
    23581 cgcccagcag catcagaaat acgtgctaat cggcaatagc tatcatgaag cggaaaaaga
    23641 gcgtcacgcc attgaggtgt taattcgcca gcgttgtaat gcgttgattg ttcactcaaa
    23701 agcattgagt gacgatgaac tggcgcaatt tatggataac attcccggta tggtgttaat
    23761 caaccgcgtt gtgccggggt acgcccatcg ttgcgtttgc ctggataatc tcagcggtgc
    23821 ccgaatggcg acgcgcatgt tgctgaataa cggtcatcaa cgtattggtt atctttcttc
    23881 cagccacggc attgaagatg acgccatgcg taaagcaggc tggatgagtg cgttgaaaga
    23941 gcaggatatt attccgccgg aaagctggat tggcactggt acgccggaca tgccgggcgg
    24001 tgaggcggcg atggttgaac tgctggggcg caatctacaa cttaccgctg tatttgctta
    24061 taacgacaat atggccgctg gcgcactgac agcattaaaa gataatggca ttgcgattcc
    24121 gttacatctc tcaatcatcg gtttcgatga tattcccatc gcccgttaca ccgacccgca
    24181 attaacgacc gtgcgttatc ccattgcttc aatggctaaa ttagccaccg aactggcctt
    24241 gcagggggca gcaggcaata ttgatcctcg tgccagccac tgttttatgc cgacgttagt
    24301 gcgtcgccat tctgtcgcaa cgcgccagaa tgcggcggcg atcactaact caacaaatca
    24361 ggcgatgtaa ccgctttcaa tctgtgagtg atttcacagt atcttaacaa tgtgatagct
    24421 atgattgcac cgttttaatg ttgtaacccg tatgtaacag tgaataatca ctttcaccga
    24481 ggtaatagcg tcataacaac aattaaagcc gttttctgga gcgttaccgg gcatggaaga
    24541 acgaatttta aaaagtgagc ttcggcgttc agtaacactt cattaactct actgccccgc
    24601 cgagcattta tctcaagcac taccctgcat aagaaaaacc ggagatacca tgaataagaa
    24661 ggtgttaacc ctgtctgctg tgatggccag catgttattc ggtgccgctg cacacgctgc
    24721 tgatactcgc attggtgtaa caatctataa gtacgacgat aactttatgt ctgtagtgcg
    24781 caaggctatt gagcaagatg cgaaagccgc gccagatgtt cagctgctga tgaatgattc
    24841 tcagaatgac cagtccaagc agaacgatca gatcgacgta ttgctggcga aaggggtgaa
    24901 ggcactggca atcaacctgg ttgacccggc agctgcgggt acggtgattg agaaagcgcg
    24961 tgggcaaaac gtgccggtgg ttttcttcaa caaagaaccg tctcgtaagg cgctggatag
    25021 ctacgacaaa gcctactacg ttggcactga ctccaaagag tccggcatta ttcagggcga
    25081 tttgattgct aaacactggg cggcgaatca gggttgggat ctgaacaaag acggtcagat
    25141 tcagttcgta ctgctgaaag gtgaaccggg ccatccggat gcagaagcac gtaccactta
    25201 cgtgattaaa gaattgaacg ataaaggcat caaaactgaa cagttacagt tagataccgc
    25261 aatgtgggac accgctcagg cgaaagataa gatggacgcc tggctgtctg gcccgaacgc
    25321 caacaaaatc gaagtggtta tcgccaacaa cgatgcgatg gcaatgggcg cggtagaagc
    25381 actgaaagca cacaacaagt ccagcattcc ggtgtttggc gtcgatgcgc tgccagaagc
    25441 gctggcgctg gtgaaatccg gtgcactggc gggcacagta ctgaacgatg ctaacaacca
    25501 ggcgaaagcg acctttgatc tggcgaaaaa cctggccgat ggcaaaggtg cggctgatgg
    25561 caccaactgg aaaatcgaca acaaagtggt ccgcgtacct tatgttggcg tagataaaga
    25621 caacttggct gaattcagca agaaataaga ctgattgggc gcagtcgttt actgcgccct
    25681 tattgtatgg ccaacaaggt aaaattatat ggtcagctca acgactccgt cttccgggga
    25741 atacttgttg gaaatgagcg gtatcaacaa gtcctttcct ggtgttaagg cacttgataa
    25801 cgttaattta aaagtccggc cacattctat ccatgcatta atgggggaaa acggtgcagg
    25861 aaaatcgaca ttattaaaat gcctgtttgg tatttatcaa aaagactccg gcaccatttt
    25921 attccagggt aaagagatcg atttccattc cgccaaagag gcactggaaa atggtatttc
    25981 gatggtacac caggagttaa acctggtatt acaacgttcg gtgatggata acatgtggct
    26041 ggggcgatat cccaccaaag gcatgtttgt cgatcaggac aaaatgtacc gcgaaaccaa
    26101 agcgattttt gatgaactgg atattgatat cgatccgcgt gcgcgcgtcg gcacattatc
    26161 cgtttcgcaa atgcagatga tcgaaatcgc caaagcgttt tcctataacg cgaaaattgt
    26221 gattatggat gaaccgactt cttcgttaac cgaaaaagag gtcaatcatc tgttcactat
    26281 tattcgtaaa ttaaaagagc gcggctgcgg tattgtttat atctcgcata aaatggaaga
    26341 aatcttccag ttatgtgatg aagttaccgt attgcgcgac ggtcagtgga tcgccaccga
    26401 accgctggca ggactgacga tggacaagat catcgccatg atggttgggc gttctcttaa
    26461 tcagcgtttt cctgacaaag aaaacaagcc gggcgaagtc atcctcgagg tgcgtaacct
    26521 aacgtcactg cgccagccgt cgattcgcga tgtctcgttt gatctgcata aaggggagat
    26581 cctcggtatt gccgggctgg tgggggcgaa acgtaccgat attgttgaga cgttatttgg
    26641 tattcgcgag aaatcggctg gcaccattac gttgcacggc aaacagatca ataaccataa
    26701 tgccaacgaa gccataaacc acggatttgc actggtaact gaggagcgcc gctcaacggg
    26761 aatttatgcc tatctggata ttggttttaa ctcgttaatt tccaatattc gcaactacaa
    26821 aaataaagtt ggtttactgg ataactcgcg gatgaaaagc gatacccagt gggtgattga
    26881 ttcgatgcgg gtaaaaacgc cgggtcatcg gacgcaaatt ggttcgctct ccggtggtaa
    26941 tcagcaaaag gtgattattg gtcgctggct actaacgcaa ccagaaatat taatgctcga
    27001 tgaaccgacg cgcggtattg atgtcggggc gaagtttgaa atttatcagt taattgccga
    27061 actggcgaag aaaggcaagg ggattattat tatctcctct gaaatgcctg agttgttagg
    27121 gataacagac cgtattctgg tcatgagcaa tggtctcgtt tccggaattg tcgatacaaa
    27181 aacaacaacg caaaacgaaa ttctgcgtct tgcgtctttg cacctttaag attaggggct
    27241 tcccatgagt gcgttaaata agaaaagttt tcttacttac ctgaaagagg gcggtattta
    27301 cgtcgttctt ttagttttgc tggcgattat tattttccag gacccaacat ttttaagtct
    27361 gttgaactta agtaatattc tcacccagtc atcggtgcgt attattatcg cgctcggtgt
    27421 ggcagggtta attgtcaccc aggggaccga tctttctgct ggtcgtcagg tagggctggc
    27481 ggcagtggtg gctgcgacat tattgcagtc catggataac gccaacaaag tgttcccgga
    27541 aatggcgacg atgccgattg cgctggttat tctgattgtc tgtgccattg gtgcggtgat
    27601 cggtttgatc aacggtctga ttatcgctta tctcaacgtg acgccgttca ttaccacgct
    27661 cggcacgatg atcatcgtct atggcatcaa ctcgctctat tacgactttg tcggggcgtc
    27721 gccaatttct ggttttgaca gtggcttctc tacctttgct cagggctttg tcgcgctggg
    27781 gagtttccgt ctctcttaca tcaccttcta cgcgttgatt gcggtggcgt tcgtctgggt
    27841 gttgtggaac aaaacccgct tcggtaagaa catttttgcc attggcggta acccggaagc
    27901 ggcaaaaata tctggtgtca acgtcggcct gaacctgctg atgatctacg cgttgtctgg
    27961 cgtgttctat gcctttggcg ggatgttaga agccggacgt atcggctctg ccaccaacaa
    28021 cctcggcttt atgtatgagc tggatgctat cgcggcgtgc gtggtaggcg gtgtatcgtt
    28081 cagcggcggt gtggggacgg tgattggcgt ggtgaccggg gtaattattt ttaccgtcat
    28141 caactatggc ctgacgtata tcggcgtaaa cccatactgg cagtacatca tcaaaggggc
    28201 gattattatc ttcgccgtag cgctggattc actgaaatac gcgcgtaaga agtgatgtga
    28261 ttgcggatat tgccggatgc gtctctggtt ttacaacgtt accgggtgtt ctttctcgcc
    28321 tttcttaaac ttcacttccc cgagctcaat acaacccacc gggcagacgt gaccacaaag
    28381 cagacaatcc acacatttct cggtattaca atgcggtgtg cgggttttct cgctccattc
    28441 catcgcctgg tgaccgccgt cgtaacagga aatataacag cgtccacagc caacacattt
    28501 gtcaagattg atacggggat agacaatata actgcggtct aaatcttccg ccgggacaat
    28561 attgttattc gccagaccta ccatctcctg caacgaatca aatccctgat cggcgagata
    28621 atgcgataac ccgctcgcca tatcttctac gatgcgatag ccgtactgca taatgccggt
    28681 ggtgacctgt aacgttgctg cgccgagcaa taaaaactcc gccgcatctt cccaggtttc
    28741 aatgccgccg ataccgctga ttgggaaatc gcgcagttct ggatgggtgc gcatttgctg
    28801 aatgaagcgc agggcgatcg gttttaccgc tttaccggaa tatccggaaa tactcgattt
    28861 accgttaacg atcggcatac cgattttctg attaagatcg atattggtga tggatttaac
    28921 ggtgttaatt gccgcaatgc catctgcgcc gccgcgcttc gccgccagcg ccacttcgca
    28981 catatcaccg atattcggcg tcatcttcgc cagcattggc agcgtggaac cccgtttcac
    29041 tgcccgacaa tatttttcta ccagctccgg gctttgcccg acatcgctac ccatcgcatg
    29101 agaagtcatt tgcggacagg agaagttaca ctcgatcata tccgcgccag ctgctcccat
    29161 tgctgctcat tttcccccat gatcgaagcg atcaatactt tgtccgggta atcttccttc
    29221 agccgacgca gggcggtcag attctcttcc aacggatgtt cagcaatctg ctccatattt
    29281 ttgaagccga taaaaccggt atcttctttc accagatgat caaaacgcgg cgagacttcg
    29341 ttggcgataa aaaagccgat cgttttaaac accacaccgc cccaacctgt gtcgtaggct
    29401 ttggcacaca tctcatagca gttgcctacc ggcgaagaag agaggcagaa cgggttggga
    29461 aagttcacgc cgcaaaaagt aatcgaaaga tctttcgtta acatgagcaa gctccctcta
    29521 aatagtgatg aatcgcctcg gcggcttctt tcccggtttt cacggcatag accacggttt
    29581 tgtcaccctc aacaatatcg ccagcagcaa agacttgcgg atcgcggatc tgataatttt
    29641 gtgttttgat ggtgttacgc tgctgctcta actccgcaaa ggcatccagt ctggcatgct
    29701 gaccgacggc gagaataatt ttatctgccg ccatcgtcag ttcgcctgat aaccgtacat
    29761 gcttaaacgt gactttattg ccttccacgg ctactggcgt gaatccatcg atgatcgaaa
    29821 cgcccagctc ccgggcgctg gcaaactctt tttcgcttgc cggaaactca tctaactctt
    29881 cacgtgctac gcaagttacc gcctgacagc cgagaacttt cagcgtgctg gctacgtcca
    29941 tcgcgacatc accgccgccg ataattaatg cgctttgtgg aatgctgata tcgccttgcg
    30001 cctgtcgtac acgttgcagg aagtcgacgg caatctcaac gtcgctatgc tcaaacagcg
    30061 gtagcccgga accgcttgat aaccccacgg tgaccagtac cgcgcggttt tccgctttca
    30121 gttgctccag ggtgagtgtt ttaccgattt cgttattgca cttaatggtc acgcccattt
    30181 tttcaatacg ggcgatctct gcatccagca ctgattgcgg taaccggaat tgcggaatac
    30241 cgttacgcaa ccagccaccg gggtgcgctt ctttctcata aatcgtgacg tcataaccct
    30301 ggtttgtcag tgtcacactg gcctgcaatc cggcaggacc tgcgccaata atcgcgactt
    30361 tgccgagcgt tttagtaccg ggctgataaa tttccatccc ggtttgttgt tcaaaatcag
    30421 taacaaaacg ctgtaagcgg ccgatatcaa tgggagcatc aacaccggca cgggtacaac
    30481 cgctttggca taatttctcc gtcgggcaca ctctggcaca aacggcaccg agggcattat
    30541 tttcgcgaat tgtctcggca gcgcctttaa aattacgaaa gtagattgag cgaataaatt
    30601 tccccggatc ggtctgtgcc gggcaagcct gactacaggg agcgtcgtga cataataaac
    30661 agcgagaggc ttctttaatc gccagtaacg gcgtaaaagc cggagtgagt tcatccagat
    30721 aattttgttg cggcatgtga tcttccttaa tgctatgagt aattatggcg aacgctaaat
    30781 aaatattgcg tgctgatatt aaggctatgc gcgtggggaa atttaagctg tgaaaggatt
    30841 cacaaatagc tgcggttcag aatgttatat ccgcagctta ttgagtgaag taataattat
    30901 tctcaataat taacagaaaa gagaaaatat taacgataga aaataagata aacaatcatg
    30961 ccgatagata aaattccgaa gccaatatat gccaccatat aagcgaagaa gagtccggga
    31021 ttttttgctc gccagacata caccggttcc ggtgcatttt taacccgttt atcaatcgcg
    31081 cctgcgcgcc agcctttcca tgcttcgcgg aaacaaaata tcgggataag gaaaaaaacg
    31141 gcaacgacaa aaaactgctg tacatccata actactttcc ttgtttcttt tgtaattcaa
    31201 gtaactgttc gggtgtaacg gcgggatacc cctgcgcatc ttccggcacc tggtgcatag
    31261 ccggaatagg gaccagcggc cctaaaaaac gcggttcacg tttaaaaata taaaggtcag
    31321 ccagcgcacc gaaacgggcg gcaaattcgc gaatacgtac tgacagcata tctttcggtg
    31381 acggtacggc ataacactga gcctgaatcc ccatatgcag cgcaataaat aatgctcgct
    31441 cacagtggaa acgttgggtg ataataatga aatcattagt atcgaaaact ttgcgtgtac
    31501 gcacgatgga atccagcgta cgaaagcctg cgtaatcgag aacaatatct gatgggtcga
    31561 caccagcagc gattaaatct ttgcgcatgg tcatcggctc gttataactt tgcaatgcgt
    31621 tatcgccgct cagtaataga taatttacct taccgctgtt ataggcatta atcgctcctt
    31681 gaatgcggta gcgataatac tgattaataa cgccggtacg ataatatttt gctgtgccga
    31741 gcaccacgcc gacctggcgg taggggagat cctgcaattc gtcgtagata taaggcgcgg
    31801 ttttccagct catccagcga tcgaggccga gcacagtcaa cagcagcaag ccgatcagga
    31861 ctaacaggct gaggaacacg cgctttaaca tgcaggagac tcgagctaag gaaaatttcc
    31921 atcaggctac tttacccgct ggttttgcgc aagaaacggc aaactgtttg ccgggaattt
    31981 cgacaccagg agagggctgg caagccggac aaggcgttca cgccgcatcc ggcaccaggc
    32041 ttaagcgaga agcacgcggt cgatactgtg acagccgaga gctttcagcg ttgcggaggt
    32101 ggcatcccac tgaatcaacg gcgcatcggc tttttctgcc agaaccgcgc gctggatatc
    32161 cgggtaatca taacccttga gattcaacag aattaacgcc ccctgcaacg gcggcagagt
    32221 cgggttgaat gcggcgtttt cagcgtagct gccgctgaaa atacgaccgt ctttacattc
    32281 cagcgcgaca ccgcttggtg acttactgta aggcatgtgc gaacggtttg ccgctgcaat
    32341 cgccgcctga gaaagcgcat caccagtcag cgcatagccg tgatcctgct cgtccatcag
    32401 cagcgtttta atctccagat ctttcggccc aaaggcatcc ggcagatagt cacgcagcgc
    32461 gtgtgcctcg cggcccggca aatgaatacg cagatccaga ccgctgttca gttcattcat
    32521 aaactgacgg cagtgaccac aaggcgtata gttaacggtg atggctgcaa gcgctttttc
    32581 accactcaac caagcgtggc tgatcgcgct ttgttcggca tgaacggttt gctgcattgt
    32641 tgcaccaata aattccatat tggcaccaaa ataccaggtt ccgctcacac cgcgcgcaat
    32701 tgcgccaaca ttaaaattcg acaatggcgt acgcgcacag gccgccgcca gcggaagtag
    32761 tgcgaatgcc agcgcgtctt cgtccagtcc cgttgcgctc ttcagcgatg agacttgctc
    32821 cccggtcaac aaagcgggga agtacttgtc tgccagaata ggttccagtg cagattgcaa
    32881 gttatccgca agttgggcaa aagcggtttg aaaacgtgga tgcatggcgt tgcctcataa
    32941 tcatgtaatg ggataacagt ttacgaaccc gttatggctt tatatgtgat ctgaatctca
    33001 ttaattatgc aacacgtgaa acttacatca aaaatgcgcg acgtatcgca aattttagcc
    33061 cattactgcc agaataatcg ggaaaaggaa cggtgcgatc agcgaagtaa ttatcccgca
    33121 taacaccagc gctagcgaac taaatgcacc ttcctgataa tccagctcgg cgcagcgcgc
    33181 cgtaccgagg gcgtgcgagg cagtccccat tgccagaccg cgcgcagctt tggtacgaat
    33241 acgcatcgca ttaagcaatg tatggccaaa taccgcgccg aggatgccga cgaaaatcac
    33301 gcaaactgcg ctgattgccg gaataccgcc aatactgccg ccaaccgcca ttgcaatagg
    33361 cgtggtgact gatttcggca ggattgacgc ggcgatttcc ggtgaagcgc ccatcaacaa
    33421 tgccacggaa gtccccgtca ccatcgcaac cacgctgccg ataaaacaga tggtgatgat
    33481 cgatttccag cgcgcgcgga tctggtgtag ctgctcatat aaaggatagg ccagcgcgac
    33541 gaccgccggt tgcagcagat cgtttagcac ttcgctacct ttaaagtaac tgtcgtaaga
    33601 gatgccggtc agcattaaaa aaggaatgat caccaccatt gctaccagca acgggttaag
    33661 caggggaaac ttataccgtg ccgccagttt gcgggcggca aaaaagacaa tcaacgtcag
    33721 cggtaatgac caccagatat tcgccatcat tcttctgatc ctttctgacc tacgacttta
    33781 cgttcaccgt gtaccagttg tgaactccag ctcaccacca gaaaaaccac cagcgtactg
    33841 acagcacagg aaaccactac cgggccaaac tgcgcgcgga gcaaatcaaa atattgcatg
    33901 acgcctacgc caatcggcac aaacaatagc gccatatagc gaatcagtac gtagcacccc
    33961 ggattgaccc atttcgccgg aagaatttgc aaggccagca ggacaaacag gatcagcatc
    34021 ccgatgatgc tgcccggaat ggttaccggt agcagggaag caatgaaaat gcctgcatac
    34081 aggcaggcat aaatcaggac gaaagcgcgt aaatattgcc agataatgtt cagtgtctta
    34141 ctcataatca acaccccaga tgagatggat tcatcataca attaattcgt aaaacgtgct
    34201 accgatcaca ttaagaaatc agcgcttatt ttttcgctac ccggtattac gcaatgagca
    34261 gcgaactcag gcatacatca agcatagaga gggattgcag tttgcagcaa tccctctttg
    34321 cagaccttta atccgctgac cataggcgct aacatagcgc ctcatttttt tgcgggtgat
    34381 gatatgcgtg tgttactggc accgatggag ggagtgcttg actctctggt gcgtgaattg
    34441 ctgaccgaag ttaacgacta cgatctgtgc atcaccgagt ttgtccgcgt ggtggatcaa
    34501 ctgctgccgg taaaagtctt tcatcgcatt tgccctgagc tacaaaacgc cagccggaca
    34561 ccatctggta cgctggtgcg cgtgcagttg ttaggtcagt tcccacaatg gctggcagag
    34621 aacgccgccc gtgcggtgga gttaggtttc tggggcgtgg atctcaattg cggctgcccg
    34681 tcgaaaacgg ttaacggtag cggcggcggg gcgacgttac tcaaagatcc tgaactcatc
    34741 taccaggggg caaaagcgat gcgtgaagct gtaccggcgc atttgcctgt cagcgtgaaa
    34801 gtgcgtctgg gctgggacag cggtgagaag aaatttgaaa tcgccgatgc ggttcaacag
    34861 gctggcgcta cggagctggt ggtgcatggg cggacgaaag agcagggtta ccgcgcggag
    34921 catattgact ggcaggcgat tggcgagatt cgccagcggc tgaatattcc ggtgattgcc
    34981 aacggtgaaa tctgggactg gcagagcgcg caacaatgca tggcgatcag cggctgcgac
    35041 gcagtgatga ttggtcgcgg ggcgctcaat attcccaacc tgagccgggt ggtaaaatat
    35101 aacgaaccgc gaatgccgtg gccggaggtg gttgctttgc tgcaaaaata tacccgtctg
    35161 gaaaagcagg gcgataccgg gttatatcac gttgcgcgga ttaaacagtg gttgagttat
    35221 ttgcgtaaag aatacgatga agcaacggaa ttatttcagc atgttcgggt gttgaataac
    35281 tcccctgata ttgcaagggc tattcaggca attgatatcg agaaactcta aaaaccatag
    35341 tgatgttttt gacaatgtgt tagctcttta tcaataggcc gcacggttgt ttggtggcgt
    35401 caaaatcgcc gaggcgttcc ctgaaggccg gggcagccca catggatgtg ggctgagggc
    35461 gcgttttaca gggatgttac ctcgcgcccg acccggtagc cgtaagggat aagccgaggg
    35521 caccgcgcag cggcgatttt gttcgccaga gcccgggggg gcagggggcg gcggcgattg
    35581 gtcgccccct gcgcgctcct tgcgccagtg attatatgaa gcaatgcaca tgacaggagc
    35641 gcaacaggat gagagactga aatattctcg ttattttcgg atctgatgac attaattaat
    35701 tttaaaatat catcttaaat acgccagtca ccaccagtag cccaatcagg aaaataatca
    35761 atacagccca gagtataatt ttcattggcg ttcctttatt tgtgagtgct gaataagtat
    35821 aggaaaaaac gaagcccttt gccgttatcc ataaacgatt aagataaatc ggaagatatc
    35881 atttacttag tctatatttt tctgggtatg tcgttttatt gctactgatg tattgccacg
    35941 cgaaactaaa aaatatatcg caacacacgg taatctccgc gcaccgtttc ttacctgatt
    36001 attcccccat gaatcgtgat tccttttatc cagccatcgc ctgttttccg ctgttactga
    36061 tgctggccgg gtgtgcgcct atgcatgaaa cccgccaggc gttaagccag caaacgcccg
    36121 ctgcacaagt tgacaccgca ttacccacgg cgctgaaaaa tggttggcca gacagccaat
    36181 ggtggctgga gtatcacgat aatcagctca cttcattaat taacaatgca ctacagaacg
    36241 caccggatat gcaggtggct gaacagcgca tttagctagc tgaagcccag gcgaaggcag
    36301 ttgccactca ggatggtccg cagatcgact tttcggcgga tatggagcgg caaaaaatgt
    36361 cggcagaagg gctaatgggg ccgtttgctc tgaacgatcc ggccgcaggt acgaccggtc
    36421 cgtggtacac caacggaact tttggcttaa cggcgggctg gcatctcgat atctggggaa
    36481 agaatcgggc ggaggttact gcccgcctgg gtacggttaa agcacgggcg gcggaacgcg
    36541 agcaaacccg ccaattgctg gctggcagcg tagcccgcct gtactgggag tggcaaaccc
    36601 aggcggcgtt aaacacggtc ttgcagcaaa tagaaaaaga gcagaacacc attatcgcga
    36661 ccgatcgcca gctatatcag aacgggatta cttcttcagt tgacggtgtg gaaaccgata
    36721 ttaatgccag caaaacccgg cagcagctca acgatgtcgc ggggaaaatg aaaattattg
    36781 aggcacggtt aaatgcactc acaaatcacc agactaagtc attgaagctt aaaccggtcg
    36841 cgttgccgaa agtggcaagc cagcttcctg atgaactggg gtactcctta ctggcccggc
    36901 gggcagattt gcaggcggcg cactggtacg ttgagtcatc gttaagcact attgatgcgg
    36961 caaaagcggc attttatcct gacatcaacc tgatggcctt cctgcaacag gatgcgttgc
    37021 acttaagcga tctgttccgt cattccgcgc agcaaatggg cgttacggca ggcctgacgc
    37081 tacctatttt cgatagtggt cgtcttaacg ccaatctcga tatcgcaaaa gccgaaagca
    37141 acttgtctat cgccagctac aacaaagcgg tggttgaagc agtgaatgac gtggcgcggg
    37201 cagccagtca ggttcagaca ctggcggaga aaaaccagca tcaggcgcaa attgagcgcg
    37261 atgccttgcg tgtggtaggt cttgcgcagg cccgctttaa cgcgggcatc attgctggtt
    37321 cccgcgtcag cgaagccaga atcccggcgc tgcgtgagcg ggccaatggc ctgttattgc
    37381 aagggcagtg gctggatgcc tccattcgac tcaccggtgc gctgggcggg ggttacaaac
    37441 gctgatgata tgcgccttct atacttaacg tttattcagc gttaagtgga gaactcgatg
    37501 gcataggtcg cgataattac cgcttccgat tcggggatcg gcaaagagtg cgcgttatta
    37561 ctggcgcagc aggggtttga tattggcatt acctggcact cagatgaaga aggggcaaaa
    37621 gataccgcgc gtgaggtggt tacccacggc gtacgtgcgg agatcgtgca actggatctc
    37681 ggcaatctgc cagaaggtgc gcaggcgctg gagacactca tccagcgatt tgggcgtatt
    37741 gatgtgctgg tgaataatgc gggtgcaatg accaaagcgc cgtttcttga tatggctttt
    37801 gatgagtggc gcaagatttt taccgttgat gttgatggtg catttttatg ctcgcaaatt
    37861 gcggctcgtc agatggtgaa ataagggcag ggcggtcgca tcatcaacat tacgtcggta
    37921 catgaacata cgccgctgcc ggatgccagc gcctacacag ccgctaaaca tgcgctcggc
    37981 gggttaacca aaacgatggc gctggagttg gtcaggcata agattttggt gaacgcagtc
    38041 gcgcctgggg cgatagccac gccaatgaac agcatggatg atagcgacgt gaagcccgac
    38101 gcggagcctt cgattcctct acggcgtttt ggcgcaacgc atgagattgc cagcctggtg
    38161 gtgtggcttt gttcggaggg cgcaaattac acgacagggc agtcgttgat agtggatggc
    38221 ggctttatgt tggcgaatcc gcagttcaac ccagaatagc gcagtgaggt gcgtggatat
    38281 ttcaatgggt taggccagat aagacaccag cgtcgcatca ggcatggaac acccggtgcc
    38341 ggatgtggag tgaatgctta tctgtggcaa ctgctttacg cctgattatc cggctttttc
    38401 ttcccgcgcc gtttcagcca ccagcgcacg cccaccacca gaaccacgac cagaatcaac
    38461 cagacccagt gcttcaaatg ctggtcgaga ttgtgcaacc acggcgcaat cacctgacca
    38521 ccagcgtaac caatagtggt aaaaatcaac gcccaggcaa atgcgccgag aatattcagc
    38581 ggcagaaaga ttttcggcgg cagctggctg gcaccaatca gcgtcgggcc aatcacccga
    38641 aagccataca taaagcgcgt accaatgaca aacagatacg gatggcgttg gataagtttc
    38701 tgtgcccgct caattttatc ctgatgtttc gagaaacggc gtaacagctt gccgccaaac
    38761 cgccgcccgc acagatagag cacctggtcg ccaatcatgc caccaagcgc cacagaaagt
    38821 accaccagtg ggaactttaa tagcccctga tgtgccgcaa cgcctcccag caaagtcacg
    38881 gtttcacctt ccgccaggct accgataacc agcgcggcat aaccatattg tgagatgagt
    38941 gtattgagat ccataggcca gtttgtctcc ctttggttgc ctgtaagcat acaccctgtg
    39001 aatcattcac gctgaaaaag gacatacatc agacggtgca aattacatgg atgaacaaaa
    39061 aataatcaca agctggctta tacttgaact gtcaggttca tgaactcaca acaagggggc
    39121 ggtatgagcc atgtctgggg actgttttcc catcccgatc gtgaaatgca ggtgattaat
    39181 cgcgaaaacg aaacgatttc tcaccattac acccaccacg ttttgctgat ggcggcgatt
    39241 ccggtgattt gcgccttcat tggcactaca cagattggct ggaattttgg cgatggcact
    39301 atcctgaagt tatcctggtt taccggactg gcgctggctg tcttgtttta cggcgttatg
    39361 ctggctgggg ttgcggtgat ggggcgggtc atctggtgga tggcgcgcaa ttacccgcag
    39421 cgtccgtctc ttgcgcactg catggtcttc gcgggctacg ttgcaactcc gctgttttta
    39481 agcggtctgg tggcgcttta tccactggtc tggttgtgtg cgctggtcgg cacggtggcg
    39541 ctgttttaca ccggctacct gctttacctt ggtattccga gcttcctgaa tatcaacaaa
    39601 gaggaaggcc ttagcttctc cagttcgacg ctggcgattg gcgtgctggt actggaagtg
    39661 ctgctcaccc taaccgttat actatggggc tacggttatc gtttgttcta gaaagttctt
    39721 tgacgttgca ttgctggcgt aaatgtcagc aatgcagcat ttactcacga ttctcctttg
    39781 acgatcgcac tttttgctcg ttatgatggc gtcgctagcc tcagtaaatc cttactccgg
    39841 cggtgcgcaa cccgtgcgcg tgaaccacta tctgaatgct catcatgccg aaatttcgag
    39901 tttctttatt tagcttggcc ctgatgctgg ctgtgccttt tgcaccgcag gccgttgcga
    39961 aaacggcagc cgctaccacc gcttcacaac cggaaattgc ctccggtagc gcgatgattg
    40021 ttgatctgaa taccaacaaa gtgatctatt cgaaccaccc ggatctggtg cgtccgattg
    40081 cgtctatcag caaattaatg accgcgatgg ttgtgctgga tgcacgactg ccgctggatg
    40141 aaaaactaaa agtggatatc agccagacgc cggagatgaa aggggtctat tcgcgcgtac
    40201 gactgaatag cgaaatcagc cgtaaagata tgctgttgct ggcgctgatg tcttcagaaa
    40261 accgcgcggc ggcaagcctt gcgcaccatt atcccggtgg ttacaaagcc tttattaagg
    40321 caatgaatgc gaaagcgaaa tcgctcggaa tgaacaacac gcgctttgtt gaacctaccg
    40381 gattgtcggt gcataacgtt tcaactgccc gtgacttaac caaactgctc attgccagca
    40441 aacaatatcc gttgatcggg cagttaagta ctacccggga agatatggca accttctcta
    40501 acccgacgta tacgctgccg ttccgcaata ctaatcatct ggtgtatcgc gataactgga
    40561 atattcagtt aaccaaaacc ggctttacca atgcggcggg ccattgtctg gtgatgcgta
    40621 cggttatcaa taataaaccg gtggcgctgg tagtgatgga cgcgtttggc aaatataccc
    40681 attttgccga tgccagccgc ctgcgtacct ggattgaaac cggtaaagtg atgcctgtgc
    40741 cggcagcagc gttgagctat aaaaaacaaa aagccgccca aatggcggcg gcggggcaga
    40801 cggcacagaa cgattaagtg aattcggatg gcgatactct gccatccgta atttttactc
    40861 cacttcctgc cagtttttcc gtttactggt tttaccgatc cccggattca tgctattggt
    40921 cggatcattc tcgcgataga acttctgcaa cgtctccggt gctttataca aatgaccgac
    40981 gttatgctcg gcagggtact gcgcgccgcg ctgctgtagc agttccagca tctgttcttt
    41041 taacgcatgc acatccacgc cttttttcac tatgtaatcc tgatggaaga catagcacat
    41101 aaaatggccg taatagagct tgtgcaccag ctggctgtcg atctccggcg gtaaatgctc
    41161 ataccactcg gtgtcgttac gccgcagagc gatatccagt gccagaatgt cttcgacttc
    41221 atcggaatgc accgcctgat aacgaattgc tgcgcccgca gcggcgaaac ggtgtaaaaa
    41281 cgctttgcta ccttcctccg gcgtacagac aaagaaatcg ccctcggcct gtttgaaata
    41341 atccaccagc cacgatttgg cttcaccgac gccatcgcct gccattttta acagcagatg
    41401 atgctcgtat ttatcgcgcc agtttttcat gcgcggcggt aaatggctgg ggaacaggtg
    41461 accgaatttt tgcattgcac ggtcagtaaa atgcggacgg aagaatttca ctttctccag
    41521 catcgcatcg gtgcgtccct tgagattaaa gaagaacggc atcttgtcgg tgcctaactt
    41581 atcaatcatc aggaaggtgt ctttgccgta tttttccgca atatcgtaga tatcccggtg
    41641 catatattcc ccggcaaccg gcagattttc gaagttagcc agaatatgac ggcggatttc
    41701 ggtcagcact tccggctggt tggtgccgat ataaaacacc tgctgatttt tttccgcttc
    41761 gaaggtatca agacgtaccg caaagacggc cagcttcccg gcgcaaccag aagattcaaa
    41821 taaccgatcc ggatcggcgt tataacgtgc gggcgtgtcg gcttcaatat cacgaacgcg
    41881 gtggacatag tcataatcgt gggcgtgacg accatcgtga cgcacatcgt catctttgat
    41941 gcgatcgtca tccagcttgc taaggatctg ctccggcgtt tcgcccagat caatccccag
    42001 atggttcacc agcgtcagtt tgccatcttc atctatacgc gcgaataacg acatttcggt
    42061 atacgccggg ccacgttgca ccagcgagcc gccggagttg ttacaaatac cgccgatgac
    42121 cgatgcgcct atacacgatg atccaatcac cgaatgcggt tcgcgtccca gcggtttgag
    42181 ggctttttcc agcgaataga gcgtggtgcc cggataggcc agcacctgtt cgcccttgcc
    42241 gagaacgtgc agcttgtcga gacgcagggt gctgatgatc acgatatcgc gatcatagtc
    42301 gttaccgttt ggcgtcgatc cttcggtcag gcctgtattg gcagcctgca tcagaataat
    42361 tttgtcggcg gtgacgcagg ctttcagcac ccgccacaat tccagtagtg agccagggaa
    42421 aacgacagcc agcgcgtcgc cctgaccaga acggaagccc ttgcgatagc gggccgtttt
    42481 tgcgggatcg gtgagcaggt gtgaatgacc gaccagacgg gcaagttcat tcaaaaaggc
    42541 tttattatca gttgttgtca tggaagacat tttccactcc ttgtggtggc gaaaaaatat
    42601 cacaaactag aatagcgcct tgtatgacaa ttcagcgagt gaatattttg aaaattcaga
    42661 caataaactg agcattcttt ttctctctcc atcatgctta tggcaaacta cggtttttgc
    42721 accattttcg ctgccacgtc gggcaacaaa ggaagaaaaa tccatatgaa atggctatgt
    42781 tcagtaggaa tcgcggtgag tctggtcctg cagccagcac tggcggatga tttattcggt
    42841 aaccatccat taacgcctga agcgcgggat gcgttcgtca ccgatctgct caagaaaatg
    42901 acggttgatg agaaaattgg tcagctgcgc ttaatcagcg taggcccgga taatccgaaa
    42961 gaggcgatcc gcgagatgat caaagacggt caggttgggg cgattttcaa caccgtaacc
    43021 cgtcaggata tccgcgccat gcaggatcag gtgatggaat taagccgcct gaaaattcct
    43081 cttttctttg cttacgacgt gctgcacggt cagcgcacgg tgttcccgat tagcctcggt
    43141 ctggcctcgt cttttaacct cgatgcggtg aaaacggtcg ggcgtgtctc tgcttatgaa
    43201 gcggcagatg atggcctgaa tatgacctgg gcaccgatgg tcgatgtctc gcgcgatccg
    43261 cgctggggac gtgcttccga aggttttggc gaagatacgt atctcacctc aataatgggt
    43321 aaaaccatgg tggaagcgat gcagggtaaa agcccggcag atcgttactc ggtgatgacc
    43381 agcgtcaaac actttgccgc atacggcgcg gtagaaggcg gtaaagagta caacaccgtc
    43441 gatatgagtc cgcagcgcct gtttaatgat tatatgccgc cgtacaaagc ggggctggac
    43501 gcaggcagcg gcgcggtgat ggtggcgctg aactcgctga acggcacgcc agccacctcc
    43561 gattcctggc tgctgaaaga tgttctgcgc gaccagtggg gctttaaagg catcaccgtt
    43621 tccgatcacg gtgcaatcaa agagctgatt aaacatggca cggcggcaga cccggaagat
    43681 gcggtgcgcg tggcgctgaa atccggaatc aacatgagta tgagcgacga gtactactcg
    43741 aagtatctgc ctgggttgat taaatccggc aaagtgacca tggcagagct ggacgatgct
    43801 gcccgccatg tactgaacgt taaatatgat atggggttgt ttaacgaccc atacagccat
    43861 ttggggccga aagagtctga cccggtggat accaatgccg aaagccgcct gcaccgtaaa
    43921 gaagcgcgtg aagtggcgcg cgaaagcctg gtgttgctga aaaaccgtct cgaaacgtta
    43981 ccgctgaaaa aatcggccac cattgcggtg gttgggccac tggcggacag taaacgtgac
    44041 gtgatgggca gctggtccgc agccggtgtt gccgatcaat ccgtgaccgt actgaccggg
    44101 attaaaaatt ccgtcggtga aaacggtaaa gtgctgtatg ccaaaggggc gaacgttacc
    44161 agtgacaaag gcattatcga tttcctgaat cagtatgaag aagcggtcaa agtcgatccg
    44221 cgttcgccgc aagagatgat tgatgaagcg gtgcagacgg cgaaacaatc tgatgtggtg
    44281 gtggctgtag tcggtgaagc acaggggatg gcgcacgaag cctccagccg gaccgatatc
    44341 actattccgc aaggccaacg tgacttgatt gcggcgctga aagccaccgg taaaccgctg
    44401 gtgctggtgc tgatgaacgg gcgtccgctg gcgctggtga aagaagatca gcaggctgat
    44461 gcgattctgg aaacctggtt tgcggggact gaaggcggta atgcaattgc cgatgtattg
    44521 tttggcgatt acaacccgtc cggcaagctg ccaatttcct tcccgcgttc tgtcgggcag
    44581 atcccggtgt actacagcca tctgaacacc ggtcgtccgt ataatgccga caagccgaac
    44641 aaatatactt cgcgttattt tgatgaagct aacggagcgc tttatccgtt cggctatggt
    44701 ctgagctata ccactttcac cgtctctgat gtgaaacttt ctgcgccgac catgaagcgt
    44761 gacggcaaag tgacggccag cgtgcaggtg acgaacaccg gtaagcgcga gggggccacg
    44821 gtagtgcaga tgtacttgca ggatgtgacg gcttccatga gtcgccctgt gaaacagctg
    44881 aaaggctttg agaaaatcac cctgaaaccg ggcgaaactc agactgtcag cttcccgatc
    44941 gatattgagg cgctgaagtt ctggaatcaa cagatgaaat atgacgccga gcctggcaag
    45001 ttcaatgtat ttatcggcac tgattccgca cgcgttaaga aaggcgagtt tgagttgctg
    45061 taatgcttta gtaagggcgc gacgtttgcg ccctttgcct gatgttcata tcttaacttg
    45121 ctgaattttc gccctttgta ggccggataa ggcgctcgcg ccgcatccgg caggaaacgc
    45181 cggaccgaat atgcctctta agtatttatc tgattctgca actgaatcct tccgctcagg
    45241 ctaactctgt cattatcaac tatgcttttc tcttaattcg ctgaaaacaa cagtaaaaga
    45301 atgaggaaag catcatgcca ctcttaaagc tctgggcagg ttcactggtt atgttggcag
    45361 ccgtgagcct gccgctacag gcggcttccc ccgttaaagt cggttcaaaa atcgataccg
    45421 aaggcgcgct gctcggcaac atcatcttgc aggtactcga aagccacggc gtaccgacgg
    45481 tgaataaagt gcaactcgga acgactcctg tggggcgcgg ggcgattact tccgatgaac
    45541 tggatatcta tccggaatat accggcaatg gcgctttctt ctttaaagat gaaaacgatg
    45601 cagcgtggaa aaatgccggg cagggctacg agaaagtcaa aaagctcgat gcggagcaca
    45661 acaagttaat ctggctgacg cccgcgcctg caaataacac ctggactatc gccgtgcgtc
    45721 aggatgtggc agagaaaaac aaactcactt cgcttgccga cctgagtcgt tatctgcaag
    45781 agggcggcac cttcaaactg gcagcctcgg cagagtttat cgaacgcgcc gatgcgttac
    45841 ccacgtttga aaaagcctat ggctttaagc tcggtcagga tcagttgctg tcactggctg
    45901 gtggcgacac ggcggtgacg attaaagccg ctgcccagca aacctctggc gttaatgccg
    45961 caatggctta cggcactgac ggtccggtcg cggcgctggg gctgcaaacc ttaagcgatc
    46021 cgcaaggcgt gcaacctatc tacgcgcctg caccagtggt gcgtgaatcg gtgttgaagg
    46081 agtatccgca aatggcacag tggctacagc cagtcttcgc cagcctcgat gcaaaaacat
    46141 tgcagcaact gaatgccagc attgcagtgg aaggactgga tgccaaaaaa gtggctgccg
    46201 actacctgaa acaaaaaggg tgggcgaagt aatttcccgt gacttatcta cgtattaatc
    46261 ctgttctggc gctgctgttg ttgctgacgg caatcgcagc ggcgctgccg tttatcagtt
    46321 acgcgcctaa tcgtttagtc tcgggtgaag ggcttcatct ctggcaactg tggccgcaaa
    46381 cgatctggat gctggtgggc gttggttgcg cctggctgac agcctgtttt gtccccgcta
    46441 aaaaaggcag catttttgca ctcattctgg cgcaattcgt cttcgtattg ttggtgtggg
    46501 gagctggaaa ggcggcgacc caactggcgc aaaatggcag tgcgctggcg cgtaccagcc
    46561 tcggcagagg tttctggctg gctgcggcgc tggcattgct ggcctgtagc gatgccatcc
    46621 gccgaatctc cacgcatccg ctgtggcgct ggttgttgca tatgcagatt gccattattc
    46681 cgctgtggtt gctgtactcc ggcacgctta acgatctctc actaatgaaa gaatacgcca
    46741 accgtcagga tgtgtttgac gacgcgctgg cacaacatct gacgttgctg tttggtgcgg
    46801 tgctgcctgc gttagtgatt ggtgtgccgt tgggcatctg gtgctacttt tccactgcgc
    46861 ggcagggggc aattttttct ctgctcaatg tcattcagac cgtgccttcg gtggcgctct
    46921 ttggcctgtt gattgcgccg cttgccgcgc tggttacggc ctttccgtgg ctggggaagc
    46981 tcggcatagc aggaaccgga atgacacccg cactgattgc gctggtgctc tatgccttgc
    47041 tgccgctggt gcgaggcgtg gtagtcggtt tgaaccagat cccgcgcgat gtgctggaga
    47101 gcgccagagc gatggggatg agcggggcgc agcgtttcct gcatgttcag ttaccgctgg
    47161 cgttaccggt atttctgcgc agcctgcggg tggtgatggt gcaaactgta ggtatggcgg
    47221 tgattgcggc gttaatcggc gcaggcggtt ttggtgcgct ggttttccag gggctgctaa
    47281 gcagcgccat tgatttagtg ttgctggggg tgatcccggt aattgttctg gcggtgctta
    47341 ccgacgcgct gttcgatttg cttatcgcac tgctgaaggt gaaacgtaat gattgaattt
    47401 agccatgtca gcaaactgtt cggcgcacaa aaagccgtta acgatctcaa tctcaatttt
    47461 caggaaggga gtttttcggt gctgattggc acatctggct ccggcaaatc caccaccctg
    47521 aaaatgatta accgcctggt ggagcatgac agcggagtga tccgctttgc cggagaagaa
    47581 attcgctcgc tgccagtact ggagttgcgc cgccggatgg gctatgccat tcaatctatt
    47641 ggcctgttcc cccactggag cgtggcgcaa aacatcgcta ccgtgccgca attacaaaaa
    47701 tggtcgcggg cgcggattga cgatcgtatc gacgaattaa tggcgctact ggggctggag
    47761 tcaaatttgc gtgagcgtta tccgcatcag ctttccggtg gtcagcagca acgtgtggga
    47821 gtggcgcgtg cactggctgc cgatccgcaa gtcttactga tggatgaacc ttttggcgca
    47881 ctggacccgg taacgcgcgg cgcgttgcaa caagagatga cgcgcattca ccgtttgctg
    47941 gggcgcacta ttgtgctggt cactcatgat attgatgagg cgctacggct ggcagaacat
    48001 ctggtattga tggatcacgg tgaagtggtg cagcagggga atccgctgac gatgctgact
    48061 cgtccggcga atgattttgt ccgccagttt tttggacgta gtgaactggg ggtgcacctg
    48121 ctttcgttac gtagcgtggc ggattacgtg cgtcgcgaag aacgggcaga aggtgaggca
    48181 ctggcagaag agatgacgct acgcgatgcg ctctccctgt ttgtcgcgcg gggatgcgag
    48241 gtgctgccgg tggtgaacac gcagggccag ccttgcggca cgctgcattt tcaggatctg
    48301 ctggtggagg cgtaagcgta tgaagatgtt gcgcgatccg ctgttctggc tcattgctct
    48361 ttttgtggcg ctgattttct ggctgcctta cagccagccg ctgtttgctg ccttgttccc
    48421 acaactgcca cgacccgttt atcagcaaga aagttttgca gctctggcac tggctcattt
    48481 ctggctggtg ggaatttcga gtttgtttgc ggtgatcatt ggcacaggtg ccggaattgc
    48541 tgttactcgc ccgtggggcg cggaatttcg cccactggtg gaaactattg ccgccgttgg
    48601 acagactttt ccgcctgtcg cagtgctggc gattgccgtt ccggtgatcg gctttggtct
    48661 gcaaccagcg attatcgcct tgatccttta cggtgtgctg cccatcctgc aggtgacact
    48721 tgccgggctg ggagcgattg atcccagcgt gacagaagtt gcgaaaggta tgggaatgag
    48781 tcgaggtcag cgactgcgta aggtcgagct accgctggcg gctccggtga ttctggcggg
    48841 cgtgcgaact tcggtgatta tcaacattgg tacggcgacg atcgcctcaa cggtaggggc
    48901 cagtacgctg ggtacgccca tcatcatcgg gcttagcgga tttaataccg cgtatgtgat
    48961 ccagggggcg ttactggtgg cactggcggc gatcatcgca gaccgcctgt ttgaaaggct
    49021 ggtgcaggcg cttagccagc acgcaaaata aaggtataac ctgcgagcat gacgccacca
    49081 attccgccta acgccataaa caggaacagg gcgatgaccc caattttagc tatgcgcata
    49141 atgcactcct tatgttaacg aaaggattgt acagtaaagc gcatttgtta acgaatcatt
    49201 aaataccgag tgggaaaata tcatggcctt gttcttgcca actggtgagt tgctgctgtt
    49261 gggcggaggt tcgattttca ccgcaccaca ccagcaatgt acggccttcg aatagttcag
    49321 ggcgtagttg attgagcgag tgggcgagcg aggacatcaa tgcgccatcc ttgttgactg
    49381 gcaatccagc cctccagcca cagacgggtg gtatcctgaa tattccagcc aaccaccagc
    49441 gcatctttac cctgtttttt acgtgccgaa gccagacaaa tggcgatgta gttgatcagt
    49501 acgccgtcga ggatcgccag cagcgcctgg agagtcggtt gttggcactg aagccgtcgg
    49561 cgcagaggaa taaacagatg tgtggtgagt gtctgggcgg ggtaatcctg accgcgctct
    49621 ttgatccacg ttcgcaggct atgtagat
//
LOCUS       DAJTUI010000021        48311 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTUI010000021 DAJTUI010000000
VERSION     DAJTUI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.021"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            187..480
                     /locus_tag="OO464_001301"
     CDS             187..480
                     /locus_tag="OO464_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6226512.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OO464_001302"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OO464_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR6226513.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="OO464_001303"
     CDS             complement(1922..2374)
                     /locus_tag="OO464_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR6226514.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="OO464_001304"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="OO464_001304"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR6226515.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="OO464_001305"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="OO464_001305"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR6226516.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="OO464_001306"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="OO464_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6226517.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="OO464_001307"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="OO464_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6226518.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="OO464_001308"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="OO464_001308"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6226519.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="OO464_001309"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="OO464_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6226520.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="OO464_001310"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="OO464_001310"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6226521.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="OO464_001311"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="OO464_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6226522.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="OO464_001312"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="OO464_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6226523.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="OO464_001313"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="OO464_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6226524.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="OO464_001314"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="OO464_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6226525.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="OO464_001315"
     CDS             complement(15085..15582)
                     /locus_tag="OO464_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6226526.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="OO464_001316"
     CDS             complement(15579..15941)
                     /locus_tag="OO464_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6226527.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="OO464_001317"
     CDS             complement(15931..16278)
                     /locus_tag="OO464_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR6226528.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="OO464_001318"
     CDS             16387..16800
                     /locus_tag="OO464_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226529.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="OO464_001319"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="OO464_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="OO464_001320"
     CDS             complement(18324..20039)
                     /locus_tag="OO464_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR6226530.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="OO464_001321"
     CDS             20206..21099
                     /locus_tag="OO464_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6226531.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="OO464_001322"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="OO464_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23526)
                     /locus_tag="OO464_001323"
     CDS             complement(21910..23526)
                     /locus_tag="OO464_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6226532.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23821..24482
                     /locus_tag="OO464_001324"
                     /pseudo
     CDS             23821..24482
                     /locus_tag="OO464_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(24521..26182)
                     /locus_tag="OO464_001325"
     CDS             complement(24521..26182)
                     /locus_tag="OO464_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6226533.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26378..26806)
                     /gene="ibpB"
                     /locus_tag="OO464_001326"
     CDS             complement(26378..26806)
                     /gene="ibpB"
                     /locus_tag="OO464_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6226534.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26918..27331)
                     /gene="ibpA"
                     /locus_tag="OO464_001327"
     CDS             complement(26918..27331)
                     /gene="ibpA"
                     /locus_tag="OO464_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6226535.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27325..27397)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27636..27968
                     /locus_tag="OO464_001328"
     CDS             27636..27968
                     /locus_tag="OO464_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6226536.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27970..29202)
                     /locus_tag="OO464_001329"
     CDS             complement(27970..29202)
                     /locus_tag="OO464_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR6226537.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            29303..30403
                     /gene="cbrA"
                     /locus_tag="OO464_001330"
     CDS             29303..30403
                     /gene="cbrA"
                     /locus_tag="OO464_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR6226538.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30364..31701)
                     /locus_tag="OO464_001331"
     CDS             complement(30364..31701)
                     /locus_tag="OO464_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6226539.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31776..32924)
                     /gene="dgoD"
                     /locus_tag="OO464_001332"
                     /pseudo
     CDS             complement(31776..32924)
                     /gene="dgoD"
                     /locus_tag="OO464_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32921..33538)
                     /gene="dgoA"
                     /locus_tag="OO464_001333"
     CDS             complement(32921..33538)
                     /gene="dgoA"
                     /locus_tag="OO464_001333"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR6226540.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33522..34400)
                     /gene="dgoK"
                     /locus_tag="OO464_001334"
     CDS             complement(33522..34400)
                     /gene="dgoK"
                     /locus_tag="OO464_001334"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR6226541.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34397..35086)
                     /gene="dgoR"
                     /locus_tag="OO464_001335"
     CDS             complement(34397..35086)
                     /gene="dgoR"
                     /locus_tag="OO464_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR6226542.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35364..36020
                     /locus_tag="OO464_001336"
     CDS             35364..36020
                     /locus_tag="OO464_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226543.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36066..36878)
                     /gene="yidA"
                     /locus_tag="OO464_001337"
     CDS             complement(36066..36878)
                     /gene="yidA"
                     /locus_tag="OO464_001337"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR6226544.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36992..37390)
                     /locus_tag="OO464_001338"
     CDS             complement(36992..37390)
                     /locus_tag="OO464_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR6226545.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37630..40044)
                     /gene="gyrB"
                     /locus_tag="OO464_001339"
     CDS             complement(37630..40044)
                     /gene="gyrB"
                     /locus_tag="OO464_001339"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6226546.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40073..41146)
                     /gene="recF"
                     /locus_tag="OO464_001340"
     CDS             complement(40073..41146)
                     /gene="recF"
                     /locus_tag="OO464_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6226547.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41146..42246)
                     /gene="dnaN"
                     /locus_tag="OO464_001341"
     CDS             complement(41146..42246)
                     /gene="dnaN"
                     /locus_tag="OO464_001341"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6226548.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42251..43654)
                     /gene="dnaA"
                     /locus_tag="OO464_001342"
     CDS             complement(42251..43654)
                     /gene="dnaA"
                     /locus_tag="OO464_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6226549.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43951..44031
                     /locus_tag="OO464_001343"
     CDS             43951..44031
                     /locus_tag="OO464_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226550.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44261..44401
                     /gene="rpmH"
                     /locus_tag="OO464_001344"
     CDS             44261..44401
                     /gene="rpmH"
                     /locus_tag="OO464_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6226551.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44418..44777
                     /gene="rnpA"
                     /locus_tag="OO464_001345"
     CDS             44418..44777
                     /gene="rnpA"
                     /locus_tag="OO464_001345"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6226552.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44741..44998
                     /gene="yidD"
                     /locus_tag="OO464_001346"
     CDS             44741..44998
                     /gene="yidD"
                     /locus_tag="OO464_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6226553.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45001..46647
                     /gene="yidC"
                     /locus_tag="OO464_001347"
     CDS             45001..46647
                     /gene="yidC"
                     /locus_tag="OO464_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6226554.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46753..48117
                     /gene="mnmE"
                     /locus_tag="OO464_001348"
     CDS             46753..48117
                     /gene="mnmE"
                     /locus_tag="OO464_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6226555.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11981 a        13060 c        12214 g        11056 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaactt ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagcaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 acggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa gcttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccgcc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttcg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcagaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat ttccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc
    23461 ggcgaaggga aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact
    23521 gagcatttcc acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg
    23581 cagacctgga gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc
    23641 gtgatcgctt tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga
    23701 catctgtgag cacaggtaca cagcatcgaa cacatcttta aaaaaaagat ttttttcaat
    23761 cgattaagca gaacttgtgg gcgcattacc cgtgcttgca ggcaaaaaag agagctagag
    23821 atgatctaca aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg
    23881 ctaaacagcc ttcttcccgg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg
    23941 actatccgta aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggc
    24001 agcggcactt acctggtgcg caaagatgtg ctgcatcaaa ccgccagcct gaccggactg
    24061 gtggaggtgt taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc
    24121 atgcctgcgc ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac
    24181 ttctcccgtc gcgttcgttt tgtggaaggg aaaccgctaa tgctggaaga cagctatatg
    24241 ccggtaaaac tgttccgtaa cctttcgctg caacatctgg aagggtcgaa gtttgaatat
    24301 attgaacaag agtgcgggat tttgattggc ggtaattatg aaagcctgat gctagactgc
    24361 tggcgcggca aatgaaggta gcggaacaca cgccactgct gcggatcacc tcgttgtcat
    24421 atagcgagag cggggagttt ttgaattatt cagtgatgtt cagaaatgcc agcgaatact
    24481 aggtggagta ccatttacgg cgactccacc cggaaaaaag ttaaccgata ctccagaaga
    24541 gcaccgccag taattggggg gtgataatgc gcaggaacat cactaacgga tagacagtgg
    24601 cgtaagagag cgccgccgca ccactggttg gatgaagatt attagcaaac gccagcgccg
    24661 gaggatcggt catggaacct gccagcatcc cgcacatggt caggtaattc attttggcta
    24721 acatccgcgc cagaatgcca acagtaatca gcggaacggc ggtgatcagg gcaccataac
    24781 caatccagct tagcccttcg ccattgacca gggtattcac aaaatcccca ccagatttca
    24841 gaccaacgac cgagaggaac agcacgatcc ccagctcccg cagcgcgagg ttggcacttg
    24901 gcggcataaa ccagtacagc ttgccgatac tgccgatacg cccgaggatc aacgccataa
    24961 tcagcggacc gcccgccagc cccagtttca acgcggccgg gaatcctggc acaaagacgg
    25021 gaatagaacc taacaatacg ccaagcccga tgccaataaa cactggcagc atctgaacct
    25081 gttgcagttt ttgctgcgca ttccccagca cattggcaac ggcatcaatt gcggacggac
    25141 gccccaccag gttgaggata tcgccgaact gcaggctgat atcgccactg gcgaccagtt
    25201 cgaccccggc acggttcagg cgcgagataa caacgtcata gcgttcttta aagtgcaggt
    25261 cgcgaatacg ttttccgagc acgttttcat tggtgaccac cacacgctcg acgcgcaaat
    25321 cagtgccttt cgtagacagc gaggtatcga cctcctgacc aatcaccagt tgcgcattat
    25381 gtaaatccgc tggctgcccc accagatgca gcaaatcgcc cagttggata atggtatctg
    25441 gcgaaggtac ttttagggtt tcttctcgtt tcagacgcga gcagataatt ttgtcgccgt
    25501 tgagaatcgg tacatcttta atggctaaat catgcaggtt agggttctca acgcgaatat
    25561 tgatagtctt gatcagcgcg ccgccattgg tgcgtgaaga ctcgtgctgc tgagcttctg
    25621 tctcgacatt gacgcggaaa ataacccgca acatccacat ggtgaacaaa atcccgcaaa
    25681 tgccgaatgg atacgccatc gcgtaactca tccccatctg atcgaccatt tccattggtg
    25741 tacccaggtc gcgcaagatc tgctgccctg cccccagcgc tggcgtattg gttaccgcac
    25801 cggagaaaat ccccagcact accggcagtg gaatatcaaa cagtttatgc aggatggcgg
    25861 taaccagacc accgatgatg acgatcagaa cagcaaacag gttgaggcgt aatccggaga
    25921 cgcgcaatga ggcaaagaag cccggcccta cctgaatccc gatggtataa acgaacagga
    25981 tcaggccaaa ttcctgaata acatgcagca tatcgctact taatgtcatc cccgcctgag
    26041 aaacaaaatg gccgacgatg atcccaccaa acagcacgcc gccaatacct aatcctatgc
    26101 cgcgaaattt gacgttgccg ataaacaaac cgacgactgc caccaaagcc agaatactga
    26161 ccgttaatgc tatatcactc atcgtccatt tctcttgaga taacatttta attgctacgg
    26221 attatggctg aatcggtgaa gcagatttgc cttatagcgc acattatcac gggaaagaac
    26281 gtgccgaaat atcttaaaca gtcgaaattt aacgcattgt tgaagaagaa agacaaaaaa
    26341 agccccacca gaatggcggg gcaaagagaa tagctagtta gctatttaac gcgggacgtt
    26401 cgctgatagc gatacgctgc gctgcgatgg gttcaggctc attacgaatt aaatcaatat
    26461 gcagtaaacc gtttacgaag gttgcgccag agacttccat attttcagcc agcgtaaaac
    26521 tcaggctaaa tggctgattc ataagccctt gatgcagcca ttttttctct tcttttggct
    26581 gctccggcgt gccttttacg ctcaggcgcg tgccttccag ttgaatctct aaatcttcct
    26641 gacggaaacc tgccagcgca agggtaatgc ggtagtggtt atcgtcgctt ttctcaatgt
    26701 tgtacggcgg gaagctctgg ctttcaccgg cgttttgcag tgcgttggcc agtttgtcaa
    26761 aaccgatcca ttgacgcatc agtggggata aatcgaagtt acgcatagtc atttctcctt
    26821 ctaagaagcg agtaagtacc tgcaaatccg aagattcgca tatgctccct gacggcgagc
    26881 atggagatgt caggccgcgc caggcggcct tagggaatta gttgatttcg atacggcgcg
    26941 gttttttcgc ttccggaatc acgcgttcga gatcgatata cagcaaacca tttaccaggt
    27001 tagccccacg aacatgaatg ttctcagcta actggaattt gcgttcaaag ttgcgttcag
    27061 cgatgccctg gtacagatag gtgcgctctt tttgttcgtc ggcgtgagca cctttcacca
    27121 ccagcagatt atcctgggcg gtaatttcca gttcgctctc agcaaaacca gccacagcga
    27181 tagcaatgcg gtaatggttt tcgtctacca gttcaacgtt atacggaggg tagccgccat
    27241 tactctggct ctggttgttt tctaagtggt taaacaaacg gtcaaatcca atagcagaac
    27301 ggtaaagcgg ggataaatca aagttacgca taatcaatag ctcctgaaat cagcgagaat
    27361 gtaagacctt ccacaatgga caggtcaggt agccagaaca cccatcaggc gcgtcctcat
    27421 cgactacgat gtaaaatggg tctggaaatg aacttttcaa gccttatcag cggacttttt
    27481 tttgcagttt atggtctatt gcatagactg agggggcagc acgaaacgtt aaagtgcgat
    27541 agccgccaca gtgcgacgta atgatgatgt tattttcagc aagggatcgc tataactcat
    27601 caggccaaca cccacgatca acagatgaat ttattatgat aagaaatgtg ttgttagcgt
    27661 tcatgatatg cagcggaatg acattactcg gaggatgctc cagcgtgatg tcccataccg
    27721 gggggaaaga aggaacgtat cccggcaccc gcgccagcgc caccatgatt ggcgacgatg
    27781 aaaccaactg gggcactaag tcgctggcga ttctcgatat gccgtttacc gctgtaatgg
    27841 acacgctttt actgccgtgg gatgtgttcc gcaaagacag ctcggtgcgc tcgcgcgttg
    27901 aaaaaagcga agccaacgcg caagccacca atgcggttat tccaccggcc agaatgcctg
    27961 ataactgatt tagcgatcag tttcggtgat ccacagctgg ccaccttcca tccacgccag
    28021 ccattgacca tccggcgaga agaccacggc gtccgcagaa ggtggattgg cgtggttttc
    28081 ggttaaatac tcaacctcgc cgctttgcgc atgggcacaa acaattcgat tatccagcac
    28141 aaagcccaac cattctcctg acggatgcca gttaaatgca gactgaatat ccgttttgtt
    28201 atgggttaac ttgcgcggtt cgccgccctg tggcgagata agccacagtt gcacaatgcc
    28261 gttatcatca cgcattaaaa acgcgatttg cgtaccctgc ggattacagc gcacccagtg
    28321 gcgcgggacg ttgactaacc ccggataagc ccgatgatgg gtaaaggtta aacgtcgctg
    28381 cacgacgcca cgcggtggcg cgggtagggt tgtttccgtt ccacttaacg gcgcatcacc
    28441 tgccgatttc cagctagctt catcttgcgg taactcaacg ataaatagct ccggcacttt
    28501 ctcgcccttt ggcgaaagtg tgtcgccaat aaacgccagc gcgtgatttc ctacccatcc
    28561 ttcttcataa gcacgattga tttcatcact gccaggctgc ggcgtgggcg tggttttact
    28621 caccagcaca caccagtggc taccgctgta ttcacgcgga tgttgctttt gtacgttgac
    28681 cgggccaaac ggtgcagcaa ccccgacgtt tcgcaaatcc agcgccggat cgagttgatg
    28741 cattacatgg tcgttatagg taaagctccc cctttcaccg ttcgggctaa agacatgcac
    28801 atggctgcca ccgcgcagcg ctcctggcgt atacggcgcg gtaatatcca ttgcatcgag
    28861 attgctcacc ttgccgcctt cagcaatgac tccgcgtcga tgatggaagt cgtaatgcca
    28921 tgtttcatca ggattttcag ggccgtgaat aaagacatat ttctctgact tcggatgaac
    28981 ggtcaccacg ccgacgtgtg cgccctgtga cgcgcgatag ataacctcga cctcgcctgt
    29041 atggatattc acacgctcaa tggtctcgcc ggtaaacgac gccccagaag gacgcacgtc
    29101 aaataccagc cactggctgt cgggcgtcca ggtattggta tttgtattgg tattggtatt
    29161 ggtgagcagg tgattacggg gagcaaaggt tatctgtttc atggcgcggt cctgatacaa
    29221 aacggttccc gccatcatac ttcacaagga cttcactttc agacaatagt cttttgccat
    29281 cgtgatgaat gtgagtgaaa agatggaaca tttcgacgtg gcgattattg gcctcggccc
    29341 ggcagggtcg gcgttggcac gaaagttagc cggcaaaatg caggtgatcg cgctggataa
    29401 aaagcaccag tgcggtactg aaggtttcag caaaccttgc ggcggtctgc tggcaccgga
    29461 cgcgcagcga tcttttattc gcgatggact gacgcttcct gtcgatgtga tcgccaatcc
    29521 acagattttc agcgtcaaaa ctgtcgacgt cgccgcatcg ctcacgcgta actaccagcg
    29581 aagctatatc aatattaatc gccatgcttt cgacttgtgg atgaaatcgc tgatccccgc
    29641 cagcgttgag gtttatcacg atagcctgtg ccgaaaaatc tggcgtgagg atgataaatg
    29701 gcatgtcatt tttcgtgcag acggctggga gcaacatatt accgcccgct atctggtcgg
    29761 tgccgatggc gcaaactcga tggtgcggcg acatctctac ccggatcatc aaatccgtaa
    29821 atatgtcgct atccagcagt ggttcgcaga gaaacatccg gtgccgttct actcctgcat
    29881 ctttgataat gcgataactg actgttactc atggagtatc agcaaagatg gttattttat
    29941 ctttggcggt gcctatccaa tgaaagacgg tcagacgcgt ttcacgacgc tgaaagagaa
    30001 aatgagcgcc tttcagttcc agtttggcaa agcggtgaaa agtgagaaat gcacggtgtt
    30061 gtttccctca cgctggcagg attttgtctg tggtaaggac aacgcctttc tgattggtga
    30121 agcggcggga tttatcagcg ccagctcgct ggaggggatt agctatgcgc tggatagcgc
    30181 agagattctg cgtgcggtgt tcctgaaaca gccggagaag agcaacgccg cttactggcg
    30241 cgccacacac aaactgcgtt taaaactctt cggcaagata gtaaaaagcc gatgcctgac
    30301 cgcaccggct ttaagaaagt ggattatgcg cagtggtgtg gcgcatattc cacagttgaa
    30361 agattatcca acgcgcttca catcacccac cagcaggatg taagagagcg cgccaatcaa
    30421 cgcgacggcg gagatataaa ccagtgcagg cgcgaaaccg taaccctgcg ccaggtaccc
    30481 caccaccagc ggaacggtga tgccgcccag accaccggcg aagttaaaca cgccgccggt
    30541 taaaccaatc aggcgcatcg gtgccagaga agagaccagc gaccaggtaa tcgaagcaaa
    30601 accgttgccg aagaatgcca gcgccatcag gcacataatc atcatcggat cgttggtgta
    30661 gttagcgccc ataatgcagg tggagatcag caagccgcag ataatcggcg ttttacgcgc
    30721 aaagcccagt gaaaagccct tacgtaccag cagatccgcg acccagccag agagcaggac
    30781 gccgacaaac gccgcgagga atggcaccgt ggtcataaag cctgctttca gcgccgtgat
    30841 tcctttttcc tgggttaaat agttcgggaa ccaggttaag aaaaaccaca gtgcagaagc
    30901 caccgcaaat tgcccaagat aaacgccgat cagtttacga tggaacacca gtttccagtc
    30961 tttggctgtt aacggctgac gcgcctcttt cttcaccggc gcatcaccat ccaccagacc
    31021 gccgccatca cgaatgtaat ccagttcagc tttgctgata cctttggtca ggcgcggcgg
    31081 ctgataaacc ttaaaccaaa tcagcgacca gataatgccg ataccaccag tgacaatgaa
    31141 cacccagtgc cagctcaaca tctcctgaat ccagatcagc agcggagtca aaaacgccag
    31201 accgacaaac tgaccagacg tataaaaacc aacggcggaa gcgcgttcat gttccgggaa
    31261 ccagctggtc accatccggt tattggtcgg gaaagcgggc gcttcgaaaa taccggttat
    31321 cgcacgcaga ccaattaatg acattaatcc cgtggcaaag ccttggaata aagtcgccac
    31381 tgaccaacca aatatcgcaa taaaataagt cacgcgagaa cctacgcgat ctaaaaacca
    31441 accgccgggg atctggcata gcgtataaag ccaggcgaag gccgaaaata catagcccat
    31501 ttccgctttc gtaatgccga actcttcctg aatatgggcg gaagccacgg ccaggttagc
    31561 gcggtcaaca taacaaatga ccaccgtaat aaagatcatc accagcgtca gataacgccg
    31621 acgccccggc tttgctgcat taacgggaat atccatagcg agctttctcc agattttggg
    31681 catagcgaag ccgctcacca tgccctgtaa tttacagagg gttatatttt tgtattgctg
    31741 ttttagtgcc cgatgagggg cttacgtggc aggaattacc actctgctac gctgttatct
    31801 tcatgacgcc agagcggatt acgccagtcc ggggcatttt tactgaactc aataacttta
    31861 gcttcgtcga tttccacacc taagcccggt ttcgttaacg gtttaaagaa gccgccaacc
    31921 atgctgaagt cttctttgtt tttcacaaag tcgagtaact ccgcgccttt gttgtaatga
    31981 atgcccatac tttgttactg aagtaccgcg ttataggaaa cgaagtcgat atgcaggcaa
    32041 gccgccagtg caatcggtcc gaacggacag tgcggcgcaa gggtcacatc ataggcttct
    32101 gccattccag caattttgta gcattcggta ataccgcctg catgggagag atccggttgc
    32161 agaatcgaaa taccgcctgc ctccagcacg cgtttaaaat cgaaacgtga gaacatgcgc
    32221 tcacccgccg ccagtggaat atgcgtttgt gccgccaatt tcgggtagta ttcggcttgt
    32281 tccgccagca ccggctcttc aataaacagc gggcgatacg gctccagttc tttaatcagc
    32341 actttcgcca ttggcgcgct gacgcgaccg tggaaatcaa gaccaaactc aatctgattg
    32401 ccaaaagctt cacgaatttg tgccacggtg ttgactgccg catctaccgc gcgggagtta
    32461 tcaattagcc ccagttcttc acaaccgttc agtttgaagg tatcgaagcc gatttcgcgc
    32521 agggttttaa tgccgtcgat aacatccgcc ggacgatcgc cgccgaccca actgtaggct
    32581 ttaattttgt cgcgaaccag gccgcccatc agttgccaga ctggcgcatt cagcactttg
    32641 cctttgatat cccataacgc ctggtcgatc ccggcgatgg cgctcatcag gattggaccg
    32701 ccacgataaa atccggcgcg atacatcact tgccataagt cgttgatgcg cgaaggatcc
    32761 tgaccaatca aatagtcacc cagctcgtga actgccgctt ccaccgtacg ggcgcggcct
    32821 tcaatcacgg gctcgcccca accgaccacg ccttcatcgg tttcaatttt caggaacatc
    32881 cagcggggag gtaaacgata cgtggtaatt ttggtgattt tcattgcact gcctctcgat
    32941 acgccttaac aaatgctgct gcctgctgcg cggtgcgttc tacggactgc ccggcgcgat
    33001 aaagatcgct gcctaagccc gccccaacac aacctgcgtc tatccactgc gccaggtttt
    33061 ctggcgtcac gccgccaacg gcaaagactg cgatgtccgg tggtaatacc gcttttaacg
    33121 ctttgatgta ttgcggacca aaagccgatg acggaaatat tttcagcgcc tgcgcgcccg
    33181 cttcgagcgc ggtaaaggct tcggtcgccg tcgcgcagcc ggggcaaacg gtcatgccgt
    33241 agcccaccgc gcggcggatc acttcactat ggatattggg cgtaacgatg agctgacagc
    33301 ccatcctggc gagcgcatcg acctgttcag gtttcagtac cgtacctgcg ccaatcaacg
    33361 ccttgtcgcc atacgcatca acgatggcgg gaatgctctg ctcccattgt ggggaattca
    33421 gcgggatttc aaccgcgtcg aacccggcgt caatcaccgc gccaacatgc gccagcgcct
    33481 cgtcgggcgt aatgccgcgc aaaatggcga tcagcgggag tttagtttgc cactgcatga
    33541 gcgatgctcc ttataccagc ctgaaatgcc gtgtcgcccg ccaccgccgc cacgtcgcaa
    33601 cccatcgcct gaaaggcttg ctggtagcgc gcggtcagcg atgttccggc gacaagggtg
    33661 atggcgtgtt gatgggtcac atagtcgcgc atactggcaa cctctgcgcc aatcaacaaa
    33721 ccagagagaa attcgctgac ctgttcgcgg ggaagtgttc ccagcacatg cgaggcgcga
    33781 acttcaaaaa gctgcggcaa tatggcgggc gcattaaggc cgcgctcaag gccagccgcg
    33841 aaggcatcgg cagagttttc ctgcggcggc aaacctgcgc caatcaatga gtgatttaac
    33901 agtaaatgat gtaattcacc ggtcatcacg gtgcgaaaat cgttgatttg ctggctatcg
    33961 gcctgcaccc atttgcaatg cgtaccgggc ataacataaa gagaggaagg agccagagct
    34021 cgcgcgccga tcaattgtgt ttcttcgccg cgcatcacat tgtggttatc gtcatgagag
    34081 acgcataatc cgggaataat ccagatattg tcgccaacag acgttaattg ttcgccaata
    34141 gacgaaaaac gggcaggaac agataaatac ggagcaactt tccagccgac attgctgccg
    34201 accattcctg ccatcaccac tggcgtattt tcttcacgcc agtcggtcgt gacttctgct
    34261 aacactgcag ccggagattt tccgttcagg cgcgtgacgc ctgcttctga ttgcctgctc
    34321 tccaggcagt ggtcgccctg ataaagccag gcgcgcagat tggtcgatcc ccagtcaatt
    34381 gcgatgtagc gagctgtcat gtgatttcct ttaaccttcg tgtcgagctg gcaatcatgg
    34441 taagcgccgc ctgctctgcc gcatcgccgt cctgatgccg tatcgcatcg aacagcgcct
    34501 tatgttcctg gagcgtttgc ggcatgttgg cctcatcgcc catccaggtt cgttcaaaaa
    34561 ccgcccgctg cagcgaactg atcgcaatgc taagttgctg taacaccggg ttatgcaccg
    34621 actgcagcac cgcctcgtgg tagcgaatat ccgcttcgtt aaacgcttcg cggtcctgat
    34681 tgttggcaat catctcgttc agcgccgatt caatctgcgc cagatcgctg gaggtcgcgc
    34741 gctctgctgc ccaacgggca atcgccggtt ccaccagatt tcgcacttcg ctcatggcac
    34801 tgataagccg tgggtcgtag tcgttttcca gcacccattg cagtacgtca gtgtcgaggt
    34861 aattccactg gttacgcggt gccacaaacg ccccgcgata acgtttcatt tcaatcagcc
    34921 gcttcgccat cagcgaacgg aacacctcac ggatgatgtt gcgcgaggtt gcaaactctt
    34981 cacagagctc cgcctcagcc gcaagtggcg agccaggtac gtatttgccg tgaacgatct
    35041 gtttacccag cgtaatgaca atgcgatcgg ttttattgag agtcatggag agtccttgtg
    35101 ctctgtatgt tcttctctac tttaccccga tcgatgcata acgcggcaac tttgtagtac
    35161 cagcgtgatg acgttcgcgt ttgccgcgcg cgtaatgtag tacaaactta tattgttgcg
    35221 ctacaattta gatcaaaaaa agaacaacac ataaaaaaat gacatgcgtc gggcagaaat
    35281 ctgaaaaggg atatcaggcg ctaaacagga gggaaagaag agtatgcttt caacggctta
    35341 gctactcgtt taaaggatta atcatgaagt tgaattttaa gggatttttt aaggctgccg
    35401 gtttattccc actggcgctg atgctttcag gctgtatctc gtatgctctg gtttcccata
    35461 ccgcaaaggg tagttcagga aagtatcaat cgcagtcaga caccatcact gggctatcgc
    35521 aggcaaaaga tagtaatgga acaaaaggct atgtttttgt aggggaatcg ctggattacc
    35581 ttatcactga tggtgccgat gacatcgtta agatgctcaa tgatccagca cttaaccggc
    35641 acaatattca ggttgccgat gacgcaagat ttgttttaaa tgcggggaaa aagaaattta
    35701 ccggcacaat atcgctttac tactactgga ataacgaaga agaaaaggca ctggcaacgc
    35761 attatggttt tgcctgtggt gttcaacact gtaccaggtc actggaaaac ctaaaaggca
    35821 caatccatga gaaaaataaa aacatggatt actcaaaggt gatggcgttc taccatccat
    35881 ttaaagtgcg attttatgaa tactattcac ccagaggcat tccggatggt gtttccgcag
    35941 cattactgcc agtgactgtt acactggaca tcattactgc accgctgcaa tttctggttg
    36001 tatatgcagt aaaccaataa tcagtaagcg ggcaaacgcg tttatgctgt ttgcccgccc
    36061 acagattaat tcagcacata cttctcaata gcaaacgcca cgccatcttc aaggttggat
    36121 tttgtgacaa agttcgccac ttctttcacc gaaggaatag cgttatccat cgccacaccg
    36181 acgcctgcat attcaatcat tgcgatgtcg ttttcctgat cgccaatcgc catgatttct
    36241 tccggtttaa tacctaacac gtcggccagt gatttcacac ccgtaccttt gttaacgcgt
    36301 ttatcgagga tttcgaggaa gtacggcgca cttttcagca cggtatattt ctctttcact
    36361 tcctgcggaa tacgcgcgat agcctggtcg aggatggcgg gttcatcaat catcatcact
    36421 ttcaggaact gggtattggg gtccattttc tccgcttcgc agaacaccag cggaattgtg
    36481 gcaacgaagg attcatgcac cgtgtagtag ctgatatcgc ggttagcggt gtacagcgtg
    36541 gtgcggtcca gggcgtggaa atgagaaccg acttcgcgag agagtttttc caggaaacga
    36601 tagtcgtcat agctgagagc ggtttgcgcc acggtgctac catcagcggc cttctgtacc
    36661 agcgcgccgt tataagtaat gcagtagtcg cccggctgtt ccatatgcag ctctttcagg
    36721 tagttgtgca cacctgcata cggacgaccc gtcgttagca cgacattcac gccacgggcg
    36781 cgagctgcgg caatcgcatt tttaacggcg ggtgaaatgg tgtgatcggg cagcagaagg
    36841 gtgccatcca tatcgatagc aatgagtttg atagccatga gttcccaagg tagattagtt
    36901 cctgacccat gctaacgcga ttccgctcaa aaatcagtac aacacccgag ggaaaagggg
    36961 atgcaacgcg ggttgcatgc tccccatttg cttagcggaa gagtttccct ttcagcagtt
    37021 ccatgcctgc ggaaagcaga tcgttattgg cttgtgctga cacttcacct tgcggtgaga
    37081 gcgcatcaat aatcttcggc aattgctctg ccagtaaact ggaagccgta ctggtatcca
    37141 cgccaagttt ttgcccgaga tcggacaccg catttgtgcc gagcgccgac tccagttgct
    37201 cgccgctaac tgattgattt ctctgttgat tactcaacca ggttgagaga atggccccta
    37261 agccgccact ttgcagtttt tccagcagca cctgaatgcc accctgctcc tcaacccaac
    37321 ttaaaatcgc ctgatatttc cccgcatcgc ctttcagaaa ggcaccgaca acttcatcaa
    37381 aaagccccat gataatcacc tgtaaagcgt tacgtgttga cccaaaaagt atagatctgc
    37441 gggatgataa ttgcggattg cagaaataaa aagggcggag atgatctccg cccttttctt
    37501 atagcttctt gccggatgcg gcgtgaacgc cttacccggc ctacaaaatc atgcaaaatc
    37561 aatatattgc aggagtctcg taggactaat aagcgtagcg catcaggcac gctcgcatgg
    37621 ttaacgccat taaatatcaa tattcgccgc tttcagggcg ttctcttcaa taaacgcacg
    37681 acgcggttct acggcgtcgc ccatcagcgt ggtgaacaac tggtcagcgg caatcgcatc
    37741 cttaacggta acgcgcagca tacgacggct ttccgggtcc atggtggttt cccacagctg
    37801 ttccgggttc atctcgccca gacctttata acgctggatg gagaggccac gacgggactc
    37861 tttcaccagc cagtccagcg cctgctcgaa gctggctacc ggctgacgac gctcgccacg
    37921 ttcgataaac gcatcttctt ccagcaagcc acgcagtttc tcacccagcg tgcagatacg
    37981 acgatattcg ccaccggtga taaactcgtg atccagcgga tagtcagtat ccacaccgtg
    38041 ggtacgcacg cgaacaatcg gctcgaacag gttttgttcg gcattggtgt ggacatcaaa
    38101 cttccactgg ctgccgtgct gttctttgtc gttcagttcg ctgaccagcg cgttcaccca
    38161 gcgggtaacg gtctgctcat cagaaaggtc agcttccgtc aacgtcggct gatagataag
    38221 ctctttcagc attgctttcg gataacgacg ctccatgcgg ttgatcattt tctgcgtcgc
    38281 gttgtactca gataccagtt tctctaacgc ttcgccagcc aatgccggtg cactggcgtt
    38341 ggtgtgcagc gttgcgccgt ccagcgcgat agagatctgg tactgatcca tcgcttcgtc
    38401 gtctttaatg tactgttcct gcttgccttt cttcactttg tacagcggcg gctgagcgat
    38461 gtagacgtga ccgcgttcaa cgatttccgg catctgacga tagaagaagg tcaacagcag
    38521 cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg atgatgatgc tgtgataacg
    38581 cagtttgtcc gggttgtact cgtcacgacc gataccacag ccaagcgcgg tgataagcgt
    38641 cgccacttcc tgagaagaga gcatcttatc gaagcgcgct ttctcgacgt taaggatttt
    38701 acccttcagc ggcagaatcg cctggttctt gcggttacgc ccctgcttcg cagagccgcc
    38761 cgcggagtcc ccttccacca ggtacagttc ggaaagcgcc ggatcgcgtt cctggcagtc
    38821 tgccagtttg cccggcaggc cagctaagtc gagcgcacct ttacggcggg tcatttcacg
    38881 cgcgcgacgc gccgcttcac gggcacgggc agcatcgata attttgccaa ccacgatttt
    38941 cgcatcagtt gggttttcca gcaggtattc cgccagcagt tcgttcatct gctgttcaac
    39001 cgccgatttc acctcagaag aaaccagttt gtctttggtc tgggaggaga atttcgggtc
    39061 cggcactttt acggaaacga ccgcaatcag gccttcacgc gcatcgtcac cggtggcgct
    39121 gactttggct tttttgctgt agccttcttt gtccatgtag gcattcaggg tacgggtcat
    39181 cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc tgcggaatgt tgttggtaaa
    39241 gcagtagatg ttttcctgga agccatcgtt ccactgcagc gccacttcaa cgccaatacc
    39301 gtctttttcg gtggagaagt agaagatatt tgggtggatc ggcgttttgt tcttgttcag
    39361 atattcaacg aacgctttga tgccgccttc atagtggaag tggtcttctt tgccgtcacg
    39421 cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac gacaactcac gcagacgttt
    39481 cgccagaatt tcatattcga actcggtcac attggtgaag gtttcgaggc tgggccagaa
    39541 acgcaccatg gtgccggttt tttcagtctc gccggtaacc gccagcggag cctgcggtac
    39601 accgtgttcg tagatctgac ggtgaatttt accctcgcgc tgaataacca gctccagttt
    39661 ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc agaccgccgg acactttata
    39721 ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc atgatcactt ccgccgccga
    39781 tacgccctct tccgggtgaa taccggtcgg aatgccgcgc ccgtcatcct gtacagagac
    39841 agagttatcg gcgtgaatgg tgacgataat ttctttacag tgacccgcga gcgcttcgtc
    39901 gatagcgtta tctaccacct cgaataccat gtggtgcaga ccggtgccgt catccgtgtc
    39961 gccgatatac atacccgggc gcttacgcac cgcatccagc cctttcagga ctttgatact
    40021 ggaggagtca taagaattcg acatcaacgt ttctcgctca tttatacttg ggttaatccg
    40081 ttattttacc cttttccacg gtaaacatct tcgaattttc gtccgacatg tctataacgt
    40141 gttcagcact gatcgcgctg acaaagacct gtgattgcgt cgcttttaag cggctggcaa
    40201 gcaacccgcg acgctcatca tcaagctcag aggcaaaatc atctatcagg tagagacacc
    40261 gccgcccgct ttcacgagtg aggaactctc cttgcgccag acgtaaggcg cacatcaaca
    40321 gcttaagctg cccacgcgat aaggtatctt ccaccggcgc accgtcggcg cgaatgcgta
    40381 agtccgcttt atgcgggcca tgcgcggtgt aggttagctg gcgatcgcgt tcaaaattac
    40441 gttccagcac ctcagcgtac tctgtctctt tctcccagcc gcgctggaaa gagaaagtca
    40501 gagaaaactc agggagaaat tgcttacagg tatcatccat atcggccgcg ataccggcgc
    40561 tatactccgc gcgccaggtg ctgatttgct ccgccagcgg gatcagttct ttatcccacg
    40621 ggcgtagctg ttcgtaacga gtcacctggc gcagcgccgc attgcgctgc ttgagcaatc
    40681 gcttgagatt gctccaggcg gtgaaaaatc caggttcgtt gtgaaagcat ccccagtcga
    40741 ggaatgctct tctgtatttg gggccgccgt tgagtaaagt aaacccttct ggcgttatca
    40801 actgcatcgg catcaggtgc gccagttccg cgactttatg cccgtccgta ccgtcaatac
    40861 ggactttgct gtcgccctgt ttgtctttgg ttaagccaat cgccgtctcg cgctcttcgc
    40921 cctgtaatcg cccgtggaga acaaatgcct cctgctcatg gcgaatgacg cgaccaatct
    40981 gcaaactgcg aaacgcccga ccatggccga gcgtatagat ggcttccagc acgctggttt
    41041 tgccactgcc gttggcacct accagaaagt taaagccggg agataaagcg agatccgcgg
    41101 tttcaatatt gcggaaatcg cggatcaaca agcgggtgag ggacattaca gtctcattgg
    41161 catgacaaca taggcagccg actgtgatgc ggcatcttca atctgcacgc tggaaaccga
    41221 atcggtcagc atcatgcgga cgttttcgca tttcagcgcg ttcagaacat ccagcacata
    41281 gctgacgttg aagccgattt ccatctccgc accgctatag gtaacgtcga ggatctcttc
    41341 cgcttcttcc tgttccgggt tgttggcagt aattttcagc tggttttcgc tgacatacag
    41401 gcgcacaccg cggaatttct cgttagagag aatcgccgcg cgagcaaacg cctgcttgag
    41461 cagatcgcag cctgcttcca gatgtttgtc cgggttcttc ggcagaacgc gacggtaatc
    41521 cgggaagcga ccatccacca gtttcgaggt gaaaataaag tcgccaacgt gggcacgaat
    41581 gttattgctg ccaatctgca cacgcagcag attgtcgccg ccgtcgagca tacgcatcag
    41641 ttcaatcacg cctttacgcg gtacgatcac cgaatggctt ggcaaagatt gaccaattgg
    41701 cattgaacag accgccagac ggtggccgtc ggttgccacg gtgcgcagtt cttcaccttc
    41761 ggtttcaaac agcataccgt ttaagtaata gcgaacatcc tgatgcgcca tcgaaaactg
    41821 ggttgcttca atcagacgct tcatcgtcgc ctgcggcagg gtaaattcga cttcgctctg
    41881 ccagtcatcg aggttcggga aatccgccgc tggcagggta gacagcgaaa aacggctacg
    41941 cccggagcgc accagcatcc gttcaccttc cagctgcacg gcaatttccg cgccttcagg
    42001 cagaccacgg cagatatcaa agaatttgcg cgccggaacg gtcgtcgctc ctggctcgtg
    42061 tggctgaacc agcgcaacgc gtgccaccat ttccatctcg agatcagtac cggtcagcga
    42121 caacgtaccg tcagcaacct gtaacagcag attaccgaga atcggtagcg taggacgacc
    42181 acctaacgga ccgctcacct gttgtagcgg ttttaataaa tgctcacgtt ctacggtaaa
    42241 tttcataggt ttacgatgac aatgttctga ttaaatttga aaaatcttct ttgatatcgt
    42301 ggctctcttc acgcaactgc tcgatcttac ggcaggcatg aagcaccgtc gtgtggtcac
    42361 gcccaccaaa cgcatcgcca atctctggca gactgtggtt agtcagctct ttcgctagcg
    42421 ccatcgccat ctggcgcgga cgcgccaccg agcgggatcg acgcttggaa aggagatccg
    42481 cgactttgat cttgtagtac tccgccaccg tcttctgaat attgtcgatg gtgaccagtt
    42541 tttcctgcaa tgccagcaag tcgcgcagcg cctcacgcac gaagtcgatg gtgatcgccc
    42601 gtccggtaaa gttggcgtta gcaataacgc ggttcagcgc cccttccagc tcacgtacgt
    42661 tagatcgtag acgcttggcg ataaagaacg ccacttcacc cggcaaacga atgtcatttt
    42721 cgtcggcctt tttcatcagg atcgccacac gggtttccag ctctggcggt tcgatcgcca
    42781 cagtcagtcc ccaaccgaag cgggatttca aacgatcctc aacgccgttg atctctttcg
    42841 gatagcgatc cgaggtgaga atgatctgtt gattaccttc cagcagggcg ttgaaggtgt
    42901 ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa ctgaatatcg tcgatcagca
    42961 gtgcatctac ggaacggtag tagcgtttaa actcttcgat cgcgttgttt tgcagggctt
    43021 taaccatgtc ctgaacaaag cgctcggagt gcatataaac cactttggca ttcggcttgc
    43081 gcgccataat gccgttaccc accgcatgca gcaggtgagt tttacccaga cccgtgccgc
    43141 cataaaggaa caacgggtta taggcaccgc cagggttatc cgccacctgg caagccgccg
    43201 cgcgcgccag ttggttagat ttaccttcaa cgaagttatc aaacgtgtgt ttgacgttta
    43261 cgttagaacg ataggtcggt tctgccgggg ccgggacgtt atcccaacct gagcgcgtag
    43321 aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc aggggccgcg acgttgctcg
    43381 tcactgccgc ttgtggcgtt tgcgtcaccg gtttggtgcc gacttcaaaa cgcagctgtg
    43441 gggcatccgc tccgcagaaa ctggttagca gtccattgat attattaagg tacttgtccc
    43501 gtacccaatc gaggacaaaa cggtttggcg cgtacagggc cagcgtgtta tcgctcagtt
    43561 ccgcctgcaa tgggcgtatc cacatactga attctgtggc tggtaactca tcctgcaatc
    43621 gggcaagaca ctgctgccaa agcgaaagtg acacggcgga ctccactcga acaaaagtcg
    43681 ataatgacta aggctgaaac attcatgatt gttgacgtac gtcgaaaaga ccctgcttga
    43741 gggtgacgca cgaaccgctg tctgcggtta tatgcccgat caagatcctg caaaacgatc
    43801 gggaccgcgg atcatagcct aaactgcgca agagatcttc tgtttctcac agattttccc
    43861 tatttatcca caggactttc cagaactcgc taagtgtaaa cgatcctgcc gcgaggcggg
    43921 cacgatttac gccgcatatt ggaaaattta atgaccatag acaaaaattg gcttaatcga
    43981 tctaataaag atccaggacg atccttgcgc tttacccatc agcccgtata atcctccacc
    44041 cggcgcgcca tgctggtttc cactggtgtg aggtcgtaca ttttccctgc gaaaaggtgc
    44101 ggaaaagcgc ggtaaataag gaaagagaat tgactccggt gtgtacaatt attacaatcc
    44161 ggcctcttta atcacccatg gcttcggtgt ccatcgtttc atttttcggc ggatatccaa
    44221 taaagccatt gaatttattc aagtttaggt agaaatcgcc atgaaacgca cttttcaacc
    44281 gtctgtactg aagcgcaacc gttctcacgg cttccgtgct cgtatggcta ctaaaaatgg
    44341 tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct cgtctgaccg tttctaagta
    44401 ataaagctaa cccctgagtg gttaagctcg catttcccag ggagttacgc ttgttaactc
    44461 ccagtcaatt cacattcgtc ttccagcagc cacaacgggc tggcacgccg caaattacca
    44521 ttctcggccg cctgaattcg ctggggcatc cccgtatcgg tcttacagtc gccaagaaaa
    44581 acgttcgacg cgcccatgaa cgcaatcgga ttaaacgcct gacgcgtgaa agcttccgtc
    44641 tgcgccaaca tgaactcccg gctatggatt tcgtggtggt ggcgaaaaaa ggggttgccg
    44701 acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt atggcgccgc cactgtcgcc
    44761 tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa cgcctgatta gtccgctact
    44821 cgggccgcat tgtcgtttca ctccaacctg ttcaagctac ggaattgagg cattgcgcag
    44881 gtttggagtg ataaaaggca gttggttgac ggtgaaacgc gtattaaaat gccacccttt
    44941 acaccctggt ggtgacgatc ccgtcccgcc cggaccattt gataccagag aacactaacg
    45001 atggattcgc aacgcaatct tttagtcatc gctttgctgt tcgtgtcttt catgatctgg
    45061 caagcctggg agcaggataa aaacccgcaa cctcaggccc aacagaccac gcagacaacg
    45121 accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg ccagtggcca ggggaaactg
    45181 atctcggtta agaccgacgt gcttgatctg accatcaaca cccgtggtgg tgatgttgag
    45241 caagctctgc tgcctgctta cccgaaagag ctgaactcta cccagccgtt ccagctgttg
    45301 gaaacttcac cgcagtttat ttatcaggca cagagcggtc tgaccggtcg tgatggcccg
    45361 gataacccgg ctaacggccc gcgtccgctg tataacgttg aaaaagacgc ttatgtgctg
    45421 gctgaaggtc aaaacgaact gcaggtgccg atgacgtata ccgacgcggc aggcaacacg
    45481 tttaccaaaa cgtttgtcct gaaacgtggt gattacgctg tcaacgtcaa ctacaacgtg
    45541 cagaacgctg gcgagaaacc gctggaaatc tcctcgtttg gtcagttgaa gcaatccatc
    45601 actctgccac cgcatctcga taccggaagc agcaacttcg cactgcacac cttccgtggc
    45661 gcggcgtact ccacgcctga cgagaagtat gagaaataca agttcgatac cattgccgat
    45721 aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg cgatgctgca acagtatttc
    45781 gcgacggcgt ggatcccgca taacgacggt accaacaact tctataccgc taatctgggt
    45841 aacggcatcg tcgctatcgg ctataaatct cagccggtac tggttcagcc tggtcagact
    45901 ggcgcgatga acagcaccct gtgggttggc ccggaaatcc aggacaaaat ggcagctgtt
    45961 gctccgcacc tggatctgac cgttgattac ggttggttgt ggttcatctc tcagccgctg
    46021 ttcaaactgc tgaaatggat ccatagcttt gtgggtaact ggggcttctc cattatcatc
    46081 atcaccttta tcgttcgtgg catcatgtac ccgctgacca aagcgcagta cacctccatg
    46141 gcgaagatgc gtatgctgca gccgaagatt caggcaatgc gtgagcgtct gggcgatgac
    46201 aaacagcgta tcagtcagga aatgatggcg ctgtacaaag ctgagaaggt taacccgctg
    46261 ggcggctgct tcccgctgct gatccagatg ccaatcttcc tggcgttgta ctacatgctg
    46321 atgggttccg ttgaactgcg tcaggcaccg tttgcactgt ggatccacga cctgtcggca
    46381 caggacccgt actacatcct gccgatcctg atgggcgtaa cgatgttctt cattcagaag
    46441 atgtcgccga ccactgtgac cgacccgatg cagcagaaga tcatgacctt tatgccggtc
    46501 atcttcaccg tgttcttcct gtggttcccg tcaggtctgg tactgtacta tatcgtcagc
    46561 aacctggtaa ccattattca gcagcagctg atttaccgtg gtctggaaaa acgtggcctg
    46621 catagccgcg agaagaaaaa atcctgattc ggtgagtttt cgctaaaata agggcggtca
    46681 gttgaccgcc ttttttcttt tcgtagggcg gataagcacc gcgcatccgc cacacaaagc
    46741 aacaggaaca tcatgagcga taatgacact atcgtagccc aggccacgcc tccgggacgt
    46801 ggcggcgttg gcatcctgcg catctccggc ttcaaagccc gtgaagttgc cgaaaccgtg
    46861 ctgggtaaac tgcctaagcc gcgctacgcc gattatcttc cgtttaaaga cgccgacggc
    46921 agcgtgctcg atcaggggat tgcgctatgg ttccctggcc cgaactcgtt caccggcgaa
    46981 gatgtgctgg aactgcaagg tcatggcggt ccggtgatcc tcgacctgct gttaaaacgc
    47041 attctgacca ttcccggcct gcggattgct cgccctggtg agttttccga acgcgcgttt
    47101 cttaacgata aacttgactt agcccaggcc gaggcgattg ccgatcttat cgacgccagt
    47161 tcggaacagg cggcccgttc ggcacttaac tcgctgcaag gcgcattctc cgcacgggtt
    47221 aatcatctgg tagaagccct cacccacttg cgcatttacg tcgaagcggc aattgatttc
    47281 cctgatgaag agatcgattt cctctccgac ggcaaaattg aagcccagct taatgacgtg
    47341 atggccgatc tcgatgcagt gcgtgctgaa gcacgtcagg gtagtttgtt gcgcgaaggg
    47401 atgaaagtag tgattgccgg acgtcctaat gccggtaaat cgagcctgtt aaacgcgctg
    47461 gcgggccgtg aagcggcaat cgtaaccgat atcgccggaa ccacgcgtga cgtgctgcgt
    47521 gagcatatcc acattgacgg aatgccgctg catatcatcg acactgccgg gctacgtgag
    47581 gccagtgacg aagtggaacg tattggtatc gagcgcgcgt ggcaggaaat tgaacaggcc
    47641 gaccgcgtgc tgtttatggt cgatggcacc acgacagacg ccgttgatcc ggcagagatc
    47701 tggccggaat ttatcgcccg tctgcccgcg aaactgccga tcaccgtggt acgcaataaa
    47761 gccgatatca ccggcgaaac gctgggaatg agtgaagtga acggtcacgc gttaattcgt
    47821 ctctcggcaa ggactggtga aggcgtggac gtgctgcgta accatctcaa acagagcatg
    47881 ggctttgaca ccaatatgga aggcggcttc ctggcgcgcc gtcgccactt acaggcgctg
    47941 gaacaggcag cggagcattt gcagcaaggt aaagcgcaac tgttgggagc ctgggcaggt
    48001 gaactgctgg cggaagagtt gcgcctggca cagcagaact taagcgaaat caccggggaa
    48061 tttacttcag acgacctgct ggggcggatt ttctccagct tctgtattgg taagtaaccg
    48121 cgcttacgaa gccgtattct gactgtcaga tgcggcttcg cttcattgtt agcactcctg
    48181 ttatgcctca accctttttt aaacattaaa attcttacgt aatttataat ctttaaaaaa
    48241 agcatttaat actgctcccc gaacgattgt gattcgattc acatttaaac aatttcagaa
    48301 tagacaaaaa t
//
LOCUS       DAJTUI010000022        47956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTUI010000022 DAJTUI010000000
VERSION     DAJTUI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.022"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO464_001349"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO464_001349"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6226556.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="OO464_001350"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="OO464_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6226557.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO464_001351"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO464_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6226558.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO464_001352"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO464_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6226559.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO464_001353"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO464_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6226560.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="OO464_001354"
     CDS             5377..5835
                     /locus_tag="OO464_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6226561.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="OO464_001355"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="OO464_001355"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6226562.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="OO464_001356"
     tRNA            6887..6971
                     /locus_tag="OO464_001356"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="OO464_001357"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="OO464_001357"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO464_001358"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO464_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6226563.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO464_001359"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO464_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6226564.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO464_001360"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO464_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6226565.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="OO464_001361"
     CDS             complement(10252..13995)
                     /locus_tag="OO464_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6226566.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO464_001362"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO464_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6226567.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="OO464_001363"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="OO464_001363"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6226568.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="OO464_001364"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="OO464_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6226569.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO464_001365"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO464_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR6226570.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="OO464_001366"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="OO464_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6226571.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO464_001367"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO464_001367"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6226572.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO464_001368"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO464_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6226573.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="OO464_001369"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="OO464_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6226574.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="OO464_001370"
     CDS             23392..23499
                     /locus_tag="OO464_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6226575.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO464_001371"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO464_001371"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6226576.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="OO464_001372"
     CDS             complement(24538..25347)
                     /locus_tag="OO464_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR6226577.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="OO464_001373"
     CDS             complement(25351..25743)
                     /locus_tag="OO464_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6226578.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO464_001374"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO464_001374"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6226579.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO464_001375"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO464_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6226580.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO464_001376"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO464_001376"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6226581.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="OO464_001377"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="OO464_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6226582.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="OO464_001378"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="OO464_001378"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6226583.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="OO464_001379"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="OO464_001379"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6226584.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="OO464_001380"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="OO464_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6226585.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO464_001381"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO464_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6226586.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="OO464_001382"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="OO464_001382"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6226587.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="OO464_001383"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="OO464_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6226588.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="OO464_001384"
     CDS             36790..39657
                     /locus_tag="OO464_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6226589.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO464_001385"
     CDS             complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO464_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR6226590.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="OO464_001386"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="OO464_001386"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR6226591.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="OO464_001387"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="OO464_001387"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR6226592.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="OO464_001388"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="OO464_001388"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR6226593.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /gene="treA"
                     /locus_tag="OO464_001389"
     CDS             45366..47063
                     /gene="treA"
                     /locus_tag="OO464_001389"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6226594.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47581)
                     /locus_tag="OO464_001390"
                     /pseudo
     CDS             complement(47142..47581)
                     /locus_tag="OO464_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12041 a        12343 c        11743 g        11829 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccacgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgtttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggaag
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgaatgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtccg cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgccagttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggacgcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtagg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgaaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcgcgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga atgagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgtcaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccta acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacaatta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaaatcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tagtaccctt ctcaaccgct actgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattctaaat gttcaataag cgcagccaaa accgcctcac
    47521 tatccgcccc atttttaacg cctgactggc tactgtctaa aatatttatt ttctgaatca
    47581 caccaaaaac aacgaaacaa taattaaaaa aggattctgt tgttgttacc gggaaccgac
    47641 ttatttcgct cagaaagtcg ttcaaccagc tccgcagtga taagattcgc tgatgataca
    47701 gtgtatcctc actgcgtgct ggaggtgcca gtataatatg ttgatcaata cagtatttat
    47761 tgtgttcagt cgtttcctga tgcgtttggt tctctgcgga ttgtcgctgg cgaaataaat
    47821 ggcttgcggt aaatgccgtt acagagcagc gcaacgcgtc aattccttcg gagtgcccga
    47881 cttttggcga gagttccggt tttatgcgtc caggtagcgg aaatttcacc cgaatgagaa
    47941 atgcattaat aaaatg
//
LOCUS       DAJTUI010000023        47333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTUI010000023 DAJTUI010000000
VERSION     DAJTUI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.023"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..480
                     /gene="sseA"
                     /locus_tag="OO464_001391"
     CDS             <1..480
                     /gene="sseA"
                     /locus_tag="OO464_001391"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR6226595.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1299..2075)
                     /gene="sseB"
                     /locus_tag="OO464_001392"
     CDS             complement(1299..2075)
                     /gene="sseB"
                     /locus_tag="OO464_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6226596.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2217..3500)
                     /gene="pepB"
                     /locus_tag="OO464_001393"
     CDS             complement(2217..3500)
                     /gene="pepB"
                     /locus_tag="OO464_001393"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6226597.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3559..3759)
                     /gene="iscX"
                     /locus_tag="OO464_001394"
     CDS             complement(3559..3759)
                     /gene="iscX"
                     /locus_tag="OO464_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6226598.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3771..4106)
                     /gene="fdx"
                     /locus_tag="OO464_001395"
     CDS             complement(3771..4106)
                     /gene="fdx"
                     /locus_tag="OO464_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6226599.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4108..5958)
                     /gene="hscA"
                     /locus_tag="OO464_001396"
     CDS             complement(4108..5958)
                     /gene="hscA"
                     /locus_tag="OO464_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6226600.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5975..6490)
                     /gene="hscB"
                     /locus_tag="OO464_001397"
     CDS             complement(5975..6490)
                     /gene="hscB"
                     /locus_tag="OO464_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6226601.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6586..6909)
                     /gene="iscA"
                     /locus_tag="OO464_001398"
     CDS             complement(6586..6909)
                     /gene="iscA"
                     /locus_tag="OO464_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6226602.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6926..7312)
                     /gene="iscU"
                     /locus_tag="OO464_001399"
     CDS             complement(6926..7312)
                     /gene="iscU"
                     /locus_tag="OO464_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6226603.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7340..8554)
                     /gene="iscS"
                     /locus_tag="OO464_001400"
     CDS             complement(7340..8554)
                     /gene="iscS"
                     /locus_tag="OO464_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6226604.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8666..9154)
                     /gene="iscR"
                     /locus_tag="OO464_001401"
     CDS             complement(8666..9154)
                     /gene="iscR"
                     /locus_tag="OO464_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6226605.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9424..10164)
                     /gene="trmJ"
                     /locus_tag="OO464_001402"
     CDS             complement(9424..10164)
                     /gene="trmJ"
                     /locus_tag="OO464_001402"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6226606.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10283..11086
                     /gene="suhB"
                     /locus_tag="OO464_001403"
     CDS             10283..11086
                     /gene="suhB"
                     /locus_tag="OO464_001403"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6226607.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11231..12085
                     /locus_tag="OO464_001404"
     CDS             11231..12085
                     /locus_tag="OO464_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6226608.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12276..13556
                     /gene="csiE"
                     /locus_tag="OO464_001405"
     CDS             12276..13556
                     /gene="csiE"
                     /locus_tag="OO464_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6226609.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13548..14687)
                     /locus_tag="OO464_001406"
     CDS             complement(13548..14687)
                     /locus_tag="OO464_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6226610.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14847..15737)
                     /gene="hcaR"
                     /locus_tag="OO464_001407"
     CDS             complement(14847..15737)
                     /gene="hcaR"
                     /locus_tag="OO464_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR6226611.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15873..17234
                     /locus_tag="OO464_001408"
     CDS             15873..17234
                     /locus_tag="OO464_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6226612.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17231..17749
                     /locus_tag="OO464_001409"
     CDS             17231..17749
                     /locus_tag="OO464_001409"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR6226613.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17749..18069
                     /locus_tag="OO464_001410"
     CDS             17749..18069
                     /locus_tag="OO464_001410"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6226614.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18066..18878
                     /gene="hcaB"
                     /locus_tag="OO464_001411"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18066..18878
                     /gene="hcaB"
                     /locus_tag="OO464_001411"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6226615.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18888..20105
                     /locus_tag="OO464_001412"
     CDS             18888..20105
                     /locus_tag="OO464_001412"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR6226616.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            20202..20624
                     /locus_tag="OO464_001413"
     CDS             20202..20624
                     /locus_tag="OO464_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6226617.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20672..21544)
                     /locus_tag="OO464_001414"
     CDS             complement(20672..21544)
                     /locus_tag="OO464_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6226618.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21556..22650)
                     /locus_tag="OO464_001415"
     CDS             complement(21556..22650)
                     /locus_tag="OO464_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6226619.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22683..23681)
                     /locus_tag="OO464_001416"
     CDS             complement(22683..23681)
                     /locus_tag="OO464_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6226620.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23706..25217)
                     /locus_tag="OO464_001417"
     CDS             complement(23706..25217)
                     /locus_tag="OO464_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6226621.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25240..26222)
                     /locus_tag="OO464_001418"
                     /pseudo
     CDS             complement(25240..26222)
                     /locus_tag="OO464_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26319..29420)
                     /locus_tag="OO464_001419"
     CDS             complement(26319..29420)
                     /locus_tag="OO464_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR6226622.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29427..29600)
                     /locus_tag="OO464_001420"
     CDS             complement(29427..29600)
                     /locus_tag="OO464_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226623.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29718..30911
                     /locus_tag="OO464_001421"
     CDS             29718..30911
                     /locus_tag="OO464_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR6226624.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31109..32362)
                     /gene="glyA"
                     /locus_tag="OO464_001422"
     CDS             complement(31109..32362)
                     /gene="glyA"
                     /locus_tag="OO464_001422"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6226625.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32689..33879
                     /gene="hmpA"
                     /locus_tag="OO464_001423"
     CDS             32689..33879
                     /gene="hmpA"
                     /locus_tag="OO464_001423"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6226626.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33924..34262)
                     /gene="glnB"
                     /locus_tag="OO464_001424"
     CDS             complement(33924..34262)
                     /gene="glnB"
                     /locus_tag="OO464_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6226627.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34323..35657)
                     /gene="glrR"
                     /locus_tag="OO464_001425"
     CDS             complement(34323..35657)
                     /gene="glrR"
                     /locus_tag="OO464_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6226628.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35647..36360)
                     /gene="qseG"
                     /locus_tag="OO464_001426"
     CDS             complement(35647..36360)
                     /gene="qseG"
                     /locus_tag="OO464_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6226629.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36525..37952)
                     /gene="qseE"
                     /locus_tag="OO464_001427"
     CDS             complement(36525..37952)
                     /gene="qseE"
                     /locus_tag="OO464_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6226630.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38510..42397)
                     /gene="purL"
                     /locus_tag="OO464_001428"
                     /gene_synonym="purI"
     CDS             complement(38510..42397)
                     /gene="purL"
                     /locus_tag="OO464_001428"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6226631.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42656..44212
                     /gene="mltF"
                     /locus_tag="OO464_001429"
     CDS             42656..44212
                     /gene="mltF"
                     /locus_tag="OO464_001429"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6226632.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44209..44745)
                     /gene="tadA"
                     /locus_tag="OO464_001430"
     CDS             complement(44209..44745)
                     /gene="tadA"
                     /locus_tag="OO464_001430"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6226633.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44770..45405)
                     /gene="yfhb"
                     /locus_tag="OO464_001431"
     CDS             complement(44770..45405)
                     /gene="yfhb"
                     /locus_tag="OO464_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6226634.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45614..46462
                     /locus_tag="OO464_001432"
     CDS             45614..46462
                     /locus_tag="OO464_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6226635.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46801..47333)
                     /locus_tag="OO464_001433"
                     /pseudo
     CDS             complement(46801..47333)
                     /locus_tag="OO464_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11282 a        12965 c        11905 g        11181 t
ORIGIN      
        1 cttgcaggct ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa
       61 ggagagttta acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg
      121 gcaagccatg aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca
      181 gaagttgatg aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt
      241 ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata
      301 ttttttggtc gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta
      361 acggcagccg tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac
      421 gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa
      481 gtatattaca ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc
      541 gcggcctttc cagcaggttg acttgtgtta catgagcaac gcaggtgctt cacagcaaaa
      601 caatactcac cagtaactct ctttttgtca agcaaaagag agtaattatt gtttatttag
      661 cgtattatcg acaccggccc tttccgccgt gttcggtaat aaaataacct ggcttattag
      721 tccgaattca gacaaatata aacaaatcct gctcaaaatt aaaaattcta accggtaaaa
      781 gatattactt aaacatgtaa atttactttc ctttaaaaaa caaaaaaccg ccaaaatcag
      841 gcggttattt gttgctggtc cggttcgcgg cctttccagc aggttgtatt accgtagtaa
      901 tgcaagcgcg tctcagcgga gacaatactc gccagtaact ctctttttgt caagcaaaag
      961 agagttatta ttgttctgtt agtgtattat ccactgcggc cctttccgcc gtctcgcaag
     1021 cgggcgctgg cttaaggaaa ggatgttccg cgaccgttag agcaagcgcg tctcagcgct
     1081 tgctatcgcg gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac
     1141 aatgaagata cggtgcttct gtatcgtact tattgtttct ggtgcgctgt tttcccaagt
     1201 aaataataac cggagtctct ccggcgacaa tttactggtg gttaacaacc ttcagagcag
     1261 caagtaagcc cgaatgccgc cctttagggc ggcatatttt agattatccg gttttgttta
     1321 aagtcacgca aaaaaccacc ccagcgacgt tcatagaatg gcgcaatatg ttcggtaata
     1381 aagtggctaa ttcctttttc cccttttttc acctgacaaa tatcgattgg ttcatcgcca
     1441 ggtaatgtat cggtcgctac acttcccgtc gcctgaataa tttcttcgat atcaccatcg
     1501 gcttcaatgc caataagtaa attaggctgt gcctcttcgt tctctttaat tgaacaaata
     1561 aaagcacgct tcaccggctt aatggtttta aataaggtgg tgagtgaatc aatcatttgt
     1621 gctggcggct ctgcgacttc cgataatatc agcgattcac cgccttccag gatttcctgg
     1681 ctgctcagcg gatttccctc ttcaccaatc aacaaactga tttcacgcgg cataaattct
     1741 ttaccggtcg gcagtttggc attaaggaag agcgtttcgc caagggtcat ctcaaacaat
     1801 gtgcgaacgg gcattacgac aaatgcctgt tcgtcttcaa ccgcctgttg aagcgcttct
     1861 aacgaggtga aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca
     1921 agcgcactat cttcaaccac agcctcgccc tgcgccgccg taccaggcac ccagacggtg
     1981 gattccagta gagtacggaa aaaggccggg cggtgcgccg gttcggttgc tgctttttcc
     2041 agcaggtctt caagttcgtt ttttgtttcg gacataagaa ccacaattca ttcaacgttc
     2101 ggggcgcaaa tgcctgatgc gctacgctta tcaggcctac aagggattcg caatttgttg
     2161 aatttgcaga atttgtaggc cggataaggc gtttacgccg catccggcat attagtttac
     2221 gccgttaaca gattagctat cgtgcgcaca ccaagtcccg tagcgcccgc agaccactgt
     2281 tcaaccggcg ctttacggta agtcgccgag cagtcgatat gcagccagcc ttgctgatag
     2341 ttctcaacaa agtgcgacag gaagcccgcc gccgtgctcg cgcctgccgg atacgccgcg
     2401 cttccggtat tgttcagttc ggcaaagtta gacggcagct ggctgcggtg gaattccgcc
     2461 agcggcagac gccagaatgg ttcgttctct tgtgaggcac tcgccagtaa acgaccggca
     2521 agcgcatcgt caaaactgaa cagcgcgtga taatcattac ccagcgcagt tttcgccgcc
     2581 ccggtgaggg tcgccgcatc aatgatcatt tccggtttct gcgcactggc atcaatcaga
     2641 ccatcggcaa gcaccagacg cccttcggca tcagtgttca tcacttcaac ttttttaccg
     2701 ttgcgatagg tgatgatatc gcccagcttg aacgcattgc cgctaatcag gttatccgca
     2761 cagcagagga acagcttcac gcgcttgttc agtccgcgca taatggcaaa tgccagcgcc
     2821 ccggtaaccg ttgccgcgcc gcccatgtcc gacttcatcg agtccataaa cgcagtctgt
     2881 ttgatgctgt agccgccgga gtcaaaagtg atacctttac ctaccaggca cgcgtacacc
     2941 ggtgcttctt tatcgccagt tgggttgtaa tccagcgcca gcaataccgg agaacgttct
     3001 gaaccgcgcc cgacagtgtg cagccccata taaccttgct cacgcagatc ttcgcctttg
     3061 gtgatccgat aagtcacacg atcgcccgcg acgttgctga tcagatcaac agcacgctgt
     3121 gccagttgcg atggtcccaa ttcttctgcc ggtgcgttga tggtgtcacg cacccagtcg
     3181 atgatcatca ggcggttatc cagttcctgg cgctgggcat cgtccagatc cggccactcc
     3241 actttacgcg tgcctttcgg gcctttataa ccttgccaga atgcccagca gcgatccgca
     3301 tcccagcctt caccgcttaa ctgaacatgc ttgatgccca gaccgtcaat cttgcgggcc
     3361 gcacgctgga tcagccccag atcgtctgcc ccgttcaaat gcagggtaat gccgtcatta
     3421 ttaatgctgt aagttgcttt ttctccccag cgcgcgtcgg caggttgggt agagagggta
     3481 atcttcatcg cttctgtcat tttagttatc cttattcgtt taacagcaaa acgggctacc
     3541 cccaggcagc ccgttcattc actcggcttc gtccagccag actaacaaaa tcgcttcgag
     3601 gattttctcg ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg
     3661 atgcatatcg gtgaatcgaa ccgttttcgg atcaagatcg ggatacgcat cgtacagtgc
     3721 ttcgccaatt tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca
     3781 cgcgcatggt tgatagtgta acgcgggatt tcgaccacta aatcttcgtc ggtgacgcgc
     3841 gcctggcagc ttaaacggct ttccggctcc agtccccagg ctttgtccag catgtcgtct
     3901 tcctgctctg agctttccgg cagtgagtca aaaccttcac gaacgatgca gtggcaggtg
     3961 gtgcaagcac aggatttttc acaggcgtgt tcaatctcaa taccgttacg cagcgctgcg
     4021 tcgagaatgg tttcaccgct attagcttcc agaacagcgc catcagggca gaggtcctga
     4081 tgaggcaaaa taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc
     4141 acgacgaacc gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt
     4201 tttaatcgct tgttcgatgg cgtcaacatc atcgccctgc gccacttcac tcaggtgagc
     4261 ggcagcattg tcaatgacct gacgctctgc ggcgcttaac agcgcggcat cagcagccag
     4321 cgcgccgtgc agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcatacg
     4381 ggcttttacg tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct
     4441 atcggtcaga ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc
     4501 catcgccgtc acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc
     4561 accgccagcc ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc
     4621 ctgcaccagt tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt
     4681 aaaggtggtg aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt
     4741 ctccaccagg ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag
     4801 catttcgctg tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc
     4861 gacgacttta tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg
     4921 ttcacgcacc agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc
     4981 agcttctaca cccgcgtctt tcagcgcgcg acgacaagcc agtaaggttc gttttaccag
     5041 tggcgcgatc agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt
     5101 aacggtcacg gagtccgcat cgctcagcgc gattttggct gcaatggtgg catccagcag
     5161 ttcacgctga acgcggttat cgctacgatc aggaatgtcc gcctgctcgc gaatgtaatc
     5221 cgccagcaga tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac
     5281 ttcaaacacg ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc
     5341 ataaacagcg atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcagt
     5401 cggttcgtta agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc
     5461 ctgacgctgg gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc
     5521 tgccagggct tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac
     5581 cgggttcagt agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa
     5641 ttgataaggc agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg
     5701 tttaacagaa ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc
     5761 caccgaatgc ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg
     5821 gtcggctaac gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc
     5881 caggtcaata ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact
     5941 aatttgtaat aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga
     6001 gttgttcggc actgctccgc agtttatcga gaaaacgcag cttacgcacc gtatccgccg
     6061 ccgcgtccca cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca
     6121 tctttttcac acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct
     6181 cgtccagctc ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat
     6241 gctgctcgct ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg
     6301 gatgacgcag cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt
     6361 gttccgccag gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa
     6421 aacgcaggct cagtgcctgg gtatcgagtt gataacgggc aggcaagcca aagagggtga
     6481 agtaatccat aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac
     6541 aaaccccacg cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct
     6601 ttcgccgcaa ccacactcat ctttaacgtt cgggttggtg aatttaaacc cttcattcag
     6661 gccttctttt acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac
     6721 cacgactttc acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa
     6781 ttccagtaca taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa
     6841 ccctttgccg cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt
     6901 aatcgacata accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa
     6961 tggcggcttt gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt
     7021 caagttcttc agcaatatcg gtgtttttga tcgcctgcgc ttcgtcgaga gacttccctt
     7081 tcacccattc ggtgaccagg gagctggaag cgatagcgga accgcagccg taagttttaa
     7141 aacgcgcgtc ttcaatgata ccttcatcgt tgactttaat ctgcaacttc attacgtcgc
     7201 cacaggccgg tgcacccacc atgccgctgc cgacgttctc gtcgttgtta tcaaaggaac
     7261 ccacgttacg cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt
     7321 ctcctgattc cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct
     7381 gcttgtacat ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta
     7441 actcgatggt gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag
     7501 agctatgtgc cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc
     7561 ttgctgacgt acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg
     7621 actcaccttc aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt
     7681 caccgttcag gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc
     7741 cgcgcagacg ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcttcgccca
     7801 ttccgacgat ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc
     7861 cgtgcatttg cgcttcgatg cgtacacgcg gtttacgacg cacatacagc gcaccgatac
     7921 ctttcgggcc atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca
     7981 ggtcgattgg cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag
     8041 cacggcacat ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt
     8101 gcatgatgga aacaaggatg gtgtcgtcac gcatcgccgc ttcgagttct ttcaggtcga
     8161 taataccgtt acgctgcggt gccaggtagg tgacttcgaa accttcgcgc tccagctgac
     8221 ggcaagtatc cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt
     8281 tctgataaaa gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg
     8341 taaagacgat ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat
     8401 ctaccgcttc ttcagcctgc cagccgaaac ggtgagaacg agaggccggg ttaccaaagg
     8461 ttccgtccat cgtcatgaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg
     8521 ttgcggagta gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc
     8581 aatgcaagga atcaggctac cggctggatg tacgaccgtg tttacggagt atttagcact
     8641 ccggcctgat tctgaattct ttttattaag cgcgtaactt aacgtcgatc gcgtcttgtg
     8701 tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt
     8761 tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat
     8821 cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcagccgcct ttaccctgac
     8881 aacgggtggc atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc
     8941 tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat
     9001 ttttacgcag acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg
     9061 aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg
     9121 tcacggcata gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc
     9181 tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt
     9241 cccgagtaaa ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg
     9301 ccattttttt atcgtggcgt ttgtacctgt tgtcggatgc ggtgtgaaca ccttatccga
     9361 cctacgattc tgtacattgt aggcctgata agacgcgtta agcgtcgcat caggcaacca
     9421 ctgttattcg gccttgttac ctttattctg ctgctcaata gaagccagaa tcccgcgcag
     9481 gatattcaat tcctggcttt ccgggcgcgc acgggtaaac agacggcgca atttattcat
     9541 cacctgcccc ggatggtttt cacggataaa accggttgcc agcagcgttt gttccagatg
     9601 accataaaaa cgctccagat catcgaccag cggatacggc gtctcttcat gctcgacctg
     9661 ctcgccattt tcctgagttg ccagccaggc catacgcact tcataggcaa taacctgaac
     9721 cgccattgcc aggttcagcg aactgtattc cgggtttgcc gcaatcgcga catgataatg
     9781 gcatttctgc aactcttcat tggtcaagcc gacgcgctca cgaccaaaca ccagcgccac
     9841 cggggtattt gccgcttcag cgacgctttt cagaccgcat tcgcgcgggt cgagcatcgg
     9901 ccacggcagc gtgcgggagc gtgcgctggt gcccaccacc aggctacaac cggctaacgc
     9961 ttcgtcgagc gtatcgacga tgtgagcatt accaatcaca tcgctggccc ctgcagccag
    10021 ggcaatcgcc tgggagtcgg gtttcaccag tggattaacc agccacagat tggttaatcc
    10081 cattgttttc atggcacggg caacagaacc catattgccg gtgtgtgacg tctccaccag
    10141 cacaattcga atattttgca gcattgtctt tcttcatcta aagattattc acgcatctta
    10201 tcataaaacg aagacagatg ccgatctcgc tgctatactc tgcgccgttt tcccgttctt
    10261 taacatccag tgagagagac cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg
    10321 caaggcgggt aatttaattg ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca
    10381 gaaaggcagt aacgatttcg tgaccaacgt agataaagct gccgaagcgg tgattatcga
    10441 cacgattcgt aaatcttacc cacagcacac catcatcacc gaagaaagcg gtgaacttga
    10501 aggtactgat caggatgttc aatgggttat cgatccactg gatggtacta ccaactttat
    10561 caaacgtctg ccgcacttcg cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt
    10621 tgctgtggta tacgatccta tgcgtaacga actgttcacc gccactcgcg gtcaaggcgc
    10681 acagctgaac ggctaccgtc tgcgcggtag caccgctcgc gatctcgacg gtaccattct
    10741 ggcgaccggc ttcccgttca aagcaaaaca gtacgccact acctacatca acatcgtcgg
    10801 caagctgttc aacgaatgtg cagacttccg tcgtaccggt tctgcggcgc tggatctggc
    10861 ttacgtcgct gcgggtcgtg ttgacggttt ctttgaaatc ggtctgcgtc cgtgggattt
    10921 cgcggcaggc gagctgctgg ttcgtgaagc gggcggcatc gtcagcgact tcaccggtgg
    10981 tcataactat atgctgaccg gtaacatcgt tgctggtaac ccgcgtgttg tgaaagccat
    11041 actggcgaac atgcgtgacg agttaagcga cgctctgaag cattaatgac tcaggcgggt
    11101 gatatcactc acccgccctc gcctttcagg cgctattccg aaatacttcc tcaccgcttt
    11161 actttctttc gtcactctcc caccatcttt ccccgattaa tggatgataa agaagtcgca
    11221 aggaaaggtt atggcactgc cagtgaacaa acgcgttccc aaaattctgt ttattctctt
    11281 tgttgttgcc ttctgcgttt atttagtgcc gcgcgttgcc atcaacttct tctattatcc
    11341 tgacgataaa atttacggtc ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa
    11401 ggacggtact cgtctgcaag gctggtttat tccttcttcg acgggccctg ctgacaacgc
    11461 catcgcaacc atcattcatg ctcacggcaa tgccggaaat atgtccgccc actggccgct
    11521 ggtcagttgg ttacctgagc gtaatttcaa cgtttttatg tttgattatc gcgggtttgg
    11581 taaatcaaaa ggcactccgt cccaggccgg attgctggac gatacgcaaa gcgccatcaa
    11641 tgtggtgcgc catcgcagtg atgtaaaccc acaacagctg gtgctgttcg ggcagagtat
    11701 tggcggggcg aatattcttg ctgttattgg tcagggtgat cgtgaaggca tacgtgcggt
    11761 gatcctcgac tccacatttg cctcttatgc aaccatcgcc aaccagatga tccccggcag
    11821 tggctactta cttgatgaga gttacagcgg cgaaaattac atcgccagcg tcagcccgat
    11881 cccgctttta ctcattcacg gtaaagccga tcacgttatc ccatggcagc acagcgaaaa
    11941 gttgtatagc ctggcaaaag agccaaaacg gttgatccta atcccggatg gcgaacacat
    12001 tgatgctttt tccgatcgtc acggcaatgt ttatcgcgaa cagatggtgg actttatcct
    12061 tagcgcgttg aatccgcaga actaacccat gatcgctagc acgataatca ttcacaaaac
    12121 caccttaaga catgctaatc cactggtcag aacagtttaa aatgagaaaa attctgtgac
    12181 gcttgccaac atttctgatg attagcattc ccttcgccat ttccctgagc aaactttagc
    12241 tattcttatc aattatgctt atgggagatc tacagatgat gcctacgctt gctccaccat
    12301 ctgtcctttc ggctccccag cgccgctgcc agatcttgct gacgctcttt cagccggggt
    12361 tgaccgccac catggcaacc ttcagcgagc ttaatggtgt ggatgatgat attgccagtc
    12421 ttgatatcag cgaaacagga cgggagatcc tgcgctatca tcaactcaca ctgacgactg
    12481 gttatgacgg tagctaccgg gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc
    12541 actggctacg acgtggtttc cgtctgtgtc cgtcatttat taccagccaa ttcacccccg
    12601 ccctgaagag tgaactgaag cggcgcggaa ttgcgcgtaa cttttacgac gataccaatc
    12661 tacaagcgtt agtgaatctc tgctcccgac ggctgcaaaa acgctttgaa tcgcgcgata
    12721 ttcatttcct gtgtctgtat ctgcaatatt gtttgctgca gcaccacgct ggaattacgc
    12781 ctcagtttaa tccgctccaa cgtcgctggg cggaatcctg ccttgaattt caggtagcgc
    12841 aggaaattgg acgccactgg cagcgtcggg cgctccagca tgtaccgcct gatgagccac
    12901 tgtttatggc attacttttt tccatgttgc gggttcccga tccactgcgg gatgcgcatc
    12961 agcgggacag acaattgcgt cagtctatca aacgtctggt aaaccatttt cgtgagctgg
    13021 gaaatgttcg tttttatgat gaacaggggt tatgtgatca gctttatatc cacctcgccc
    13081 aggcgttaaa tcgcagtttg tttgccatcg gtattgataa taccctgccg gaagagttcg
    13141 ccagactgta tccacgcctg gtacgcacca cccgcgcggc gctggccgga tttgaaagtg
    13201 aatacggcgt ccatctttct gatgaggaaa gtggtctggt cgcggtgatt ttcggtgcct
    13261 ggctaatgca ggaaaacgat ctgcatgaaa aacagattat tctactgacc gggaatgata
    13321 gcgagcgaga agcgcagatt gagcagcagt tacgcgaact aacgttactg ccgctcaaca
    13381 ttaagcatat gtcggtaaag gcatttttgc agacaggcgc tccgcgtggc gcggcactga
    13441 ttattgcgcc ttataccatg ccgttaccgc tcttttcacc accgctgatc tatacggacc
    13501 tgacgctgac aacacatcaa caggagcaga tccgcaaaat gctcgaatca gcatgaggga
    13561 acaactttcg ggcgcaaaaa cattgccgga agcgccacca gcgccattac ccagaacacg
    13621 ccgtggccca gatattgata caggaaaccg gcgaaaacgg tcatgatagc gatactgccg
    13681 cccatcgcga cggcagagta aaccgcctgt aaacggatga cttcgctaac ctggcgagca
    13741 gcaatataac gcatggcggc caggtggcag accgtgaagg tgccgcaatg cagaatttgc
    13801 accactatca accacggcaa cgccgtagtt gctcccataa tgccccagcg cactacgccg
    13861 caaatcgccg agatcaacag catatcgcga gcactacaac ggcggaaaag tttattactc
    13921 agcgcaaaga taatgacttc cgccaccacg cccagcgacc acaaataccc caccgccgag
    13981 gccgagtagc cagctgcctg ccagtaaatg gcgctaaaac cgtaataggc cgcatgtgcc
    14041 ccctgcaata aacaaacgca ggccagaaag cgccagttct ggcgaaccag cgccaaccac
    14101 gcagaccaac cggtgctctc ctgctggcgg cttgcccctt gtggctgaat cgtcggacgg
    14161 atgagaaagc cgagcagcat ggatgccacg cccaacgtca acagcacgag gatcacccga
    14221 taatcaaaca tagtgaccag tttgcccgtc agcgccgagc caatgacaaa cgccaccgag
    14281 ccccacagtc gcactttgcc gtaatcaagc gggaactgct tttgccacgt attcgccagt
    14341 gcatcggtca acggtaccag cggtgagaaa aagaggttaa agccaatcat caccagcatc
    14401 agccacgcta cgtgcgcccc cgcccagaag gcgacagcaa agagaagtgg cagcagtgcc
    14461 agcacgcgca aggcggaaat caggcgggaa ggatcgctga cgcggggcgc gatgagcaaa
    14521 ctcccgagaa aacgggcaac cagacctgcc cccaataaca ggccgatggt ttctggcgtt
    14581 aaaccaatcc ctttaagcca gacgctccag aaaggtaaaa aaatgccgta actaaaaaag
    14641 tatgtgaaat agccgagcgc caaccagcgc gtggattgca aaaccatgag tccctcccgt
    14701 caggaggcgt tagtctgggg ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc
    14761 gagcctgctt cccgatgtct gtaacgccag ttcacaagca ctaaaaaact gcgatccgcg
    14821 acgcatgttg caaaaataat gcgtaattat gccgttacgc ttgccaaacg ttcctgcaca
    14881 atggcgatga aatcgcgtaa tgccagcttc atctcccctt tcttccacgc catcagtaaa
    14941 gcaatggaag gcacatttcc ggcaatggga cgaaaaacaa cctgcccggt attaaaatta
    15001 ttcatataac cgggtatcaa agtgacgccc agccccatgc ccaccagatt catggtcacc
    15061 agaatattcg ttgccacctg gacgatattt ggctggctgt tttcttgcgc aaaccacgct
    15121 ttaacgatcg gcgcaagcga accggaatac gccggatcgg tactgacgaa attcacgcca
    15181 tccagttgcg cggcggtgat ctctttttca tgtgctaaag ggtgatcaac cggtaacacc
    15241 accactaatg gttcgtcaaa aagctccaga taatcaatct ccgggctata aacaggatgg
    15301 cgcattaagc cgacgtcgag ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt
    15361 aaactcacca gctcaatcaa ggtgtctggc tgtctgagac gaaacatcgg taatactttt
    15421 ggcaataaat tcacttccgc cgatggcaca aagccaatgg ttaattgtct gtcttcctga
    15481 acaattttcc gcgcccgtaa tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc
    15541 tggagaaaac attctcccgc cgccgtcaac gcgactttgc gcttatccct caccagtaac
    15601 ggaacaccga cacagttttc aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta
    15661 tgcagttttt ccgccgcacg ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt
    15721 aaatgccgta gttccatcac cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa
    15781 ttaactattt cacatattag caaccaacca gcaacatcct tatggcacaa aaatagaagg
    15841 tcaatacatc ttatctttca ggattaaaaa atatgaccac accctcagat ttgaacattt
    15901 accaactgat tgacacccaa aacggtcggg tcactccgcg tatttatacc gacccggaca
    15961 tttaccaact ggagcttgag cgtattttcg gtcgttgctg gctatttctc gcccacgaaa
    16021 gccagatccc aaaacccggt gatttcttta acacctacat gggagaagat gcagttgtcg
    16081 tggtgcgcca gaaagacggc agcatcaagg cgtttctcaa ccaatgccgc caccgggcca
    16141 tgcgtgtgag ttatgcagat tgtggcaaca gtcgcgcctt tacctgcccg tatcacggct
    16201 ggtcttatgg cattaacggc gagttgatcg atgtaccgct ggaacctcgc gcctacccac
    16261 aagggttgtg taaatcccac tgggggctaa acgaagttcc ttgtgtggag agttataaag
    16321 gactgatttt tggcaactgg gataccagcg caccgggcct gcatgattac ctgggtgaca
    16381 ttgcctggta tctggatggc atgctggatc gtcgcgaagg cggcaccgaa attgtcggcg
    16441 gcgtacaaaa gtgggtgatc aactgtaact ggaaattccc ggcagagcag ttcgccagtg
    16501 accagtatca tgctctgttc agccatgctt ctgccgttca ggtattaggg gcgaaagatg
    16561 atggcagcga taagcgcctc ggtgatggac aaaccgcccg cccggtgtgg gaaaccgcca
    16621 aagatgcgct gcaatttggt caggacggtc acggtagcgg tttcttcttt actgaaaaac
    16681 cggatgctaa tgtctgggtc gatggcgcag tttcaagcta ttaccgcgaa acctatgccg
    16741 aagcagaaca acgtttaggt gaagttcgcg ccctgcgcct ggcgggtcat aacaatatat
    16801 tccccacgct ttcatggcta aacggcactg ccacactccg cgtctggcat ccgcgcggcc
    16861 ctgatcaagt tgaagtgtgg gcgttctgta ttactgacaa agccgcctcc gatgaagtta
    16921 aagccgcttt tgaaaacagc gccactcgtg cttttggtcc tgctggtttt ctcgagcagg
    16981 atgactcgga gaactggtgt gaaatccaga aattgcttaa aggccaccgc gcccgcaaca
    17041 gcaaactgtg tctggaaatg gggcttggtc aggaaaagcg ccgcgacgac ggcattcctg
    17101 gcattactaa ctatatcttt tcagaaactg ccgctcgtgg aatgtaccaa cgctgggccg
    17161 atcttctgag tagcgaaagc tggcaggaag tgctcgataa aaccgccgct taccagcagg
    17221 aggtgatgaa atgagtgcgc aagtttcact ggagttacat caccgcatta gccagtttct
    17281 ctttcacgaa gccagtctgc tggatgactg gaaatttcgt gactggttgg cacagctcga
    17341 cgaagagatt cgttacacca tgcgcaccac ggttaacgcg caaacacgcg accgtcgcaa
    17401 aggcgtccag ccgccgacga cctggatttt caatgacacc aaagaccagc tggagcggcg
    17461 aatcgcccgt ctggaaacgg gcatggcctg ggcagaagag ccgccgtcac gcactcgtca
    17521 cttaatcagc aactgccagg taagcgaaac cgacatccca aacgtatttg ccgtgagggt
    17581 gaattatcta ctttatcggg cacaaaaaga gcgcgatgaa acattctatg ttggaacgcg
    17641 tttcgacaaa gttcgccgtc tggaagatga caactggcgc ttgctggaac gggatatcgt
    17701 cctggatcaa gcggtgatca cttcccataa cctgagtgta ctgttctgat gaatcgaatt
    17761 tatgcgtgtc ccgttgcgga tgtgccggag ggtgaggctc tccggataga tacctcgccc
    17821 gtcatcgcct tgttcaacgt gggcggcgag ttttatgcca ttaacgatcg ttgcagccat
    17881 ggtaatgcgt cgatgtcaga agggtatctg gaagatgacg ccacggtgga gtgcccgcta
    17941 cacgccgcca gtttttgcct gaaaacgggg aaagcgttat gcctgcccgc caccgatccg
    18001 ctcaccactt atccagtaca cgttgaaggt ggtgacattt tcatcgactt accggaggcg
    18061 cagccatgag cgatctgcat aacgagtcca tttttattac cggcggcgga tcgggattag
    18121 ggctggcgct ggtcgagcga tttatcgaag aaggcgcgca agttgccacg ctggaactgt
    18181 cggcggcaaa agtcgccagt ctgcgtcagc gatttggcga acatattctg gcggtggaag
    18241 gtaacgtgac ctgttatgcc gattatcaac gcgcggtcga tcagatcctg actcgttccg
    18301 gcaagctgga ttgttttatc ggcaatgcag gcatctggga tcacaatgcc tcactggtta
    18361 atactcccgc agagacgctc gaaaccggct tccacgagct gtttaacgtc aacgtactcg
    18421 gttacctgct gggcgcaaaa gcctgcgctc cggcgttaat cgccagtgaa ggcagcatga
    18481 ttttcacact gtcaaatgcc gcctggtatc ctggcggcgg tggcccgctg tacaccacca
    18541 gtaaacatgc cgcaaccgga cttattcgcc aactggctta tgaactggca ccgaaagtgc
    18601 gggtgaatgg cgtcggcccg tgtggtatgg ccagcgacct gcgcggccca caggcgctcg
    18661 ggcaaagtga aacctcgata atgcagtctc tgacgccgga gaaaattgcc gccattttac
    18721 cgctgcaatt tttcccgcaa ccggcggatt ttacggggcc gtatgtgatg ttggcatcgc
    18781 ggcgcaataa tcgcgcatta agcggtgtga tgatcaacgc tgatgcgggt ttagcgattc
    18841 gcggcattcg ccacgtagcg gctgggctgg atctttaagg aagcacgatg aaagaaaaaa
    18901 cgatcattat tgtcggtggc gggcaagcgg cggcaatggc tgcggcctcg ctacgccagc
    18961 aagggttcac cggtgagctg catctgtttt ccgatgagcg acatcttcct tatgaacgac
    19021 ctccactctc gaaatccatg ttgctggaag attccccgca gttacagcag gtgttacccg
    19081 ctaactggtg gcaggaaaac aatgttcatc tgcattccgg tgtaaccatc aaaacgctgg
    19141 gtcgcgacac acgagagtta gtgttaacca acggcgaaag ctggcactgg gatcagcttt
    19201 ttatagcaac cggcgcggca gctcgaccgc tgccgttgct tgatgcactg ggagaacgct
    19261 gctttaccct acaccatgcc ggtgatgccg ccagactgcg agaagttctg cagcccgaac
    19321 ggtcagtcgt gattgtcggt gccggaacta ttggtctgga actggctgcc agcgccacgc
    19381 agcgcagcgc agcgcagcgc agatgtaagg tgacagtgat tgaactggcg gcaaccgtca
    19441 tgggccgtaa tgcaccaccg cccgtgcaac gctatctttt acagcgccat cagcaggctg
    19501 gtgtgcgcat tctgctcaat aatgccattg aacatgtggt cgatggtgaa aaagtagaac
    19561 tgacgctgca aagtggggaa acgcttcagg ctgatgtggt gatttacggt attggtatca
    19621 gcgccaacga gcaactggct cgcgaggcca accttgatac tgccaatggc attgtcattg
    19681 atgaggcttg ccgcacctgc gatcccgcga tctttgccgg tggcgatgtg gcaatcactc
    19741 gtcttgataa tggtgcacta caccgctgcg aaagctggga aaacgccaat aaccaggcgc
    19801 aaattgccgc tgccgcaatg ttagggctac cgctaccgct actgccgccg ccgtggttct
    19861 ggagcgatca gtacagtgat aacttacagt ttattggcga tatgcgtggc gatgactggc
    19921 tttgtcgtgg caacccggaa actcagaagg cgatttggtt taatctgcaa aacggcgtgc
    19981 ttatcggtgc ggtcacgctg aatcagggcc gtgagattcg cccaattcgc aaatggatcc
    20041 agagcggcaa aacgtttgat gcgaaactgc tgatagatga gaacatcgcg cttaaatcac
    20101 tgtaatcagg ataattagcg aatatctcaa tgcctggggc gtggcgaggt gcaagagtgt
    20161 gtattacgtt taaatcacat tatcttgcaa agggattggt tatgaacaca ctacgttatt
    20221 ttgattttgg agctgcccgc cccgttttgt tattaattgc ccgtatcgcc gtggtcttaa
    20281 ttttcattat ttttggtttt cccaaaatga tgggctttga cggtacggtc caatatatgg
    20341 cctcgttggg cgcgccaatg ccgatgctgg cagcgattat tgcggtagtt atggaagtgc
    20401 ccgccgcgat attaatcgtg cttggctttt tcacccgtcc gctggcggtg ctgtttattt
    20461 tctacacgct gggtacggcg gtgattggtc accattactg ggatatgacc ggcgatgcgg
    20521 ttgggccaaa tatgattaat ttctggaaga atgtcagtat cgctggcgcg ttcttgctat
    20581 tggcaattac cggaccgggg gcaatttctc tcgatcggcg ttaggaaaga tgccggatgc
    20641 ggcgtgaacg ccttatccgg cattttataa attacagcca ctctacccgc aacgacatct
    20701 ctgaggttgt tgattcccct ggtgctagcg caatgagatc acccccttcc gggcgatgat
    20761 gctcatccgg cgcatggctc atcggttcca gacagaagaa atcaaacgca tatcctttat
    20821 caaacgcagg gtcggaaaca aagatgaaat aacacggtgc aggtggcgtc gtttccatga
    20881 tgatggcata accttcctgc ggctgctcga tgcgggcctg accattccat ccggcaaaac
    20941 cattgttcac ccactgacgc ggcaacggcg cgagctggtt aaaatccagt tcctgcggta
    21001 gctgctcgca aaactcaccc gccagccact gctcccgttc cagccagtaa ccgctcgcct
    21061 gcgcctgaat ccgcgtttgt ggtgacaacg gaaaataagg atgccagccg gtaccaaatg
    21121 gcagcgtctc tgccccttga ttggtgacag agagcgtcac cgtcagcgta tccgccgtta
    21181 aatgaaacgc ctgacttacc cgatagtgat agacaccgct gcgatgttca tacaccagac
    21241 acaaactatc atcgctgtgc gagacacatt gccactcgcc cagccagcca tcgccgtgta
    21301 gatagtgcgc atcccactca acattcggtt gcagttgata ctcacgcccc tgccagacaa
    21361 aacggttgcc gctcacccgg tttgcaaatg gcaccagcgg aaaacatgag gcatcggttg
    21421 ccacaccgct ttttttacca ggacgtaaga gcggcgtcgt atcgcgccag aagccttcga
    21481 ttacgccgcc ctggtcagaa acgtccagct ttagcgaccc gtgggataag gtatagatgg
    21541 tcatccgcta actccttaat ccgggaagtt aatcacaact ttcccgcatt tgccgctcgc
    21601 catcagcaca taggcatcac ctgcctgttc tagcgagaag cgatgggtaa tggcgttacg
    21661 cggccacagc ttccagtcag tcaaatcgtg ggcgcatttt tccatatgga acagactggt
    21721 cacccaggag ccgataatcc gccgttgatg gtgcatcaga tcagcgctga cctcgaattc
    21781 cacttttccg gtttcaccaa tgtaaaccac ccgcccccag tcagcagtgg attgcagtgc
    21841 cagcaagcga cctgcggcat taccggaaca atcgagcgca acatccgcgc caccgtgggt
    21901 aagttcagca attatctgcg gcagaccttc ggtggttgct aaatagccgt gatccatccc
    21961 ccctaactgt tttgccatcg ccagacgttc cggcagcata tcaacgccga tgatccgttt
    22021 tgcaccgcga cctttcgcca gcatcatcgc catcatgccg accggcccca gaccgaccac
    22081 cagcacgtta tcgctgccgg aaacttcgcc gcgcaaaatc ccttcatagg ccgtgccaac
    22141 gccgcaactg ataaacgcac catcttcgta gctcagcgca tccggcagga ggatcagatc
    22201 tttttcttcc gccagcagat attcggcatg accgccgtca cgctgccagc cgtaagccgc
    22261 ttttcctttg ccagtacaag agataggaaa gccgcggcgg cagttcgggc aaaaaccaca
    22321 gccagaaatg tgatacacca gcacgcggtc gccctcttta aaatggcggc agccttgccc
    22381 catcgccaca atctgcccgc acggttcatg accgttgata aatccctggt ataacggttt
    22441 atcgggtgcc gccgccgtgg cacggtgttg atgatagata tagtggacat cgcttccgca
    22501 aatcccggag gatttcattt tgatcagtac ctggttaatc cccggcgtcg gcaccgcaac
    22561 ttcccgcaga tcgacggttg aatttcctag taaataagca gccagcatcg ttttcataaa
    22621 tccctcatta acaatacgat taattttcat ccctgcagca cccgcgccag gggcgttaag
    22681 gtttagcgtt tcgctttact gcttcgctgg gtcagcaaga tattcgccag caccgccacc
    22741 acgatgatga cgccgcgtac cacctgctgg aaaaaggagt taataccgag cagcaccaga
    22801 ccgttaccga ttagcgtaat caccagcaca ccaagcaatg taccgaacag ggaaccgcga
    22861 ccgccggaaa gtgccgtacc gccgaccacg accgcggcga tgacgtcaaa ctccagaccg
    22921 tttgcggcac ctgcgttacc ggaaccgagg cgtgccgcca acaaaatgcc ggtcaccgcc
    22981 gctaataatc ccgaaagggt aaagataaga atgcgcactc gacgaacgtt aataccgcac
    23041 aactgcgccg ccgtggcatt accgccaacg gcaaaaaccg agcgcccgaa ggcggttttt
    23101 cggctaatga acacaaacag cgcaaacaac accatcatga tcagcgcgga tactggcaca
    23161 ccgagaaatt gtccgcccag ccagtccagc acctcgtttt cgtcaatcgg cactggcagt
    23221 gcgttggtca taaacagccc cattccgcgc agagcgctcc acagccccag tgtggcaacg
    23281 aaacttggta cgttaaacac gccgcgcagc accccggcga gcgttcccat cagcgcgcct
    23341 aacagcaaca ccagcagaca agcaatcgcc aacggaactt caaattgcag caaaaatgcc
    23401 aggcacaccg agacaaaagc caccatcggc ccaacgctga catcaatttc accggagata
    23461 ataatcagcg tcatcgccca ggcggcaatc ccaatggtgg cggcatcgcg cagcacgttc
    23521 atctggttat tcaatgagat aaagccaggc gcgttcaggg agaagaccag ataaagaatg
    23581 gcaatcacca ccagcaaacc gatctcatta atatggcgac tgacaaattg tttgagcgag
    23641 acgctcttgc cctgcggcaa tggtaatgac gaagcataca tcttcaggtt cctcggtaaa
    23701 attcatcagt gcacagacag aatggcggac atcagctcat ccacattgac cggcgagtga
    23761 aactcctgcg agaacgtgcc gtgctgtaat aacaggatgc ggtcacacac cagcggcaat
    23821 tcctccactt cactggagat aaacaccacg ctttttcctt cggcagccag ctcacggaca
    23881 atacggtaaa tctgctgttt ggcttcgata tcgacgccgc gcgttggctc gtcgagcaac
    23941 aaaatctggc tggcggcata gacccaacga ccgatcacca ctttttgctg attgccacca
    24001 gaaagcgtgc cgatgggtgt ttcgctactg gcagccttga ccgtcatccg ctgcatcacc
    24061 tcttcggtca ggcggcggat ggtggaccat tgcagcacac cgttggtgct gattttttgc
    24121 cgattggtca gcactgtatt ttcgtcaacg cctagccagg gaatgatccc cgcttctttg
    24181 cggttttctg gcgtatagcc aatgccgcgt ttcagcatgt cgccgtaatc ggggcgcatg
    24241 attttctcgc cgttgataac aatttcgccc tgttcatacg tctccagccc aacaatcgcc
    24301 ttcagcaatt cactgcgccc tgcccccagc aggccagcaa tgccaagcac ttcgccacga
    24361 cgtagcgtaa agctgatatc ctccagcttg ggcttatggc gtaacgcacg gacttccagt
    24421 acggcctgat ccacaatttc ctgaggggca accggcgcaa tatcaacgtg atcacgccct
    24481 agcattagcg acacaatatg atgcgtggag gtgttttcga gcatcacatc gcccgccacc
    24541 tgaccatcgc gcataacggt ggcataggag gcaatgcggc ggatttcttc catccggtgg
    24601 ctgacataaa tcaccgccac gcccagtgcc gacatctttt tcaccgcact gatcaccagt
    24661 tcaacttctg cactggcaag cgagctggtg ggttcatcaa gaatgaccac gcgcggctcg
    24721 cccttcatca cccgtgcaat ttccaccagt tgcttttgcg ccgggcttag cgttgaaaca
    24781 agttgttcag gactaacgtc aacgcccagc gcctgtaagc aacgttgggc atcctgcgcc
    24841 atttgcaggt aatcaatcat gccgttgcgg cgaggccatt gaccaaggca gaggttttct
    24901 gccaccgtca gcccttccac cagacttaat tcctgataaa ccgcacgaac ccccagttca
    24961 gcggcacggc gagtcaacgt agcttcgtca ccttccagtc gcgtctcgcc aatccagata
    25021 tcaccgctat ccgggcgttc gctgccggta agcattcgaa tgagagtcga tttgcccgcg
    25081 ccgtttttac ctaacagcgc acgaacttcg cctttattga gcgtgaagtt aacgttatcc
    25141 agcgcaacga cgccggggta acgcttattt cctgccacca cttttgctac cgggactgcc
    25201 tctgttgccg tgaacatagg gacctctgcg aatcagcgat tagggcagac catcaacgtg
    25261 cgttgccagc cactgtttac catcttccgt tttggtatag agatcgattg gcacctgaat
    25321 cactttttca ccgtcggctt gtttattgat aaccttcaat gtttgcgcga aaacagcatt
    25381 gcccattttc ttaccggaaa tatccactac cgctttcagc acctgattgt tttccagctc
    25441 ctgagcaatt tcggttgtca tatccgaacc gaaaacagca atttttccgg cctgattttg
    25501 attacgtacc gcttttaccg cgccgagtgt cgcaccgccc gactccccca taatggcgtt
    25561 gagatccggc gtggagataa tcagtttttc accaacggaa atcgctttat ctaaaacagt
    25621 cccttcctgg ttagcgacaa tttgcgcgcc gggaacgcgg gattttaata cttcttcaaa
    25681 tcctttacgt cgctgcacac aaacttcaaa ggcttcgcaa ttgatgacgg caattttcgg
    25741 ctggtcaatt ttattggcaa taaaataatc ggcggcagcg ttacccagtt tttaccaaat
    25801 tccagtggat cgccgaccag atacgccgag acatatttat cgacaccctt ttgattaata
    25861 caggtgttgt agcaaatcac cggaataccc gcttcactgg cgcgacgaac ggtacggcta
    25921 ctgccatttt cagacactgc cgataaaata atggcatcga cattacgcgc cacgagggta
    25981 tcaacaaagg tactttcttt cgaaatatcc ccctgggcgt tagtttcaat taactgtacc
    26041 tgtactgaag aatcttttgc cgcatcctga acgccctgac gcactccagc gtaatatccc
    26101 tgggtatcaa ggtatattgc accaatggtc atttctttat ccgcagccct ggcaaatagt
    26161 gcgctaccta atagcgtagc cattaataat agattacggg ttgttctcat ttttttaggc
    26221 atagtgcctc ctgtagggtt tttattaaca acggcttatt ctaattattt tgtgatgagc
    26281 ggcagcgcgt gccgctcaaa tattacagtt aatagggttt agtgaacgta gttaaaaata
    26341 aaaggcatca cagtggtgaa taatgccgct ttcggccagg ccggattaat cgccgtcaat
    26401 tcagcgcgag cagactcata ttgcgcaacc tccccgagtc ccgcggacgc cagcatcgcc
    26461 accatcaggc atttttcttt atgctgctgt tgtaaatcgc catacagcga taacaggtca
    26521 ggttgtgaaa cagcaaagaa atccgcctca atactggttt tcgccatctc ttgcgcccac
    26581 tgtttcattt cactaaacag ttgctgtgcg gtttgctgtt cacccagcag tcgcagcgcc
    26641 attccttgcc agaagagata atcaaccggc tgatcgttgt aataactgtg gatgttaatg
    26701 gtgcgatcgc cggtcgccgc cagacgtaaa caacgcatcg cttcagtttc atcgccctgc
    26761 gcgttggcgc atatcgcctg ccagaaccag atgtcgttat cagtttgccc cggtaaacgg
    26821 ccttcgctta aattctccgg ataatacagc gcggcatgaa gcagttcgct ggcctgctgc
    26881 ggctctctgg catcaagatg ctgccaggcg cgtaataact ggttgaggat aaactgactg
    26941 gtgaccttcc cttccccgcc ttcccacggg tggaatttac gcgtggcgag aatgtccgcc
    27001 gctttgtctg cctgacccgt gagatgccac aaattgagca gttctgcggt catgtcgtcg
    27061 cgtttcagcg caatttccag attattttcc agacgcgcca gtcgtttctc cggtgtggct
    27121 ccacttaact tatccagcaa atcccgttcg aaaagcagac gtgcatcctg cggcgcaagc
    27181 tgataagcat tatcaagata acgcgcggcc agctcataat cgtgtttctt attccacgca
    27241 tggatcgcta acccgcgcca gccgtcggca aactccggcg acatctctac gcaacgttgc
    27301 cataaggcaa tggctttgtt gtagctacgt ttgttgtagt agaagcaggc cagtaaatgg
    27361 cgagcaaacc aacactcttc aatactctcc agcgccgcca cttcttccag cgtattcggg
    27421 aaacggacaa actgcgggaa gacatcaatg gcttttgcga ccagttcgcc acgttcggct
    27481 ttcggcagca agctggcttg caggtaaagc ggcagcgtgc gctggcagtc cagtgcgttc
    27541 agcatctctg ccgccagggt gggcattccc cagttaatca gttgcccggc ggtcatcaga
    27601 gcgttaacgt cgcgtccctg acacagaccg cgccactgcg cgagggctga ttcgctacga
    27661 ccatcgaacc agttcagcca ccacagagtg gcgttcagcg gataatcacg cagcagtttc
    27721 tcgcgctgca aacgcgcgtt gtcctgacga ccacttaaca ccagcagcag attatgcaag
    27781 caaagcactt cctgattggt tgggcaggca cgaagacttt gttggcaaaa atccagaccc
    27841 gcgtcgaagt tatcattacg cgccgccagt cgtgccagac cataatagcc gcctgcttta
    27901 ctattgccgc tccagaccgc acgccagaaa tcctcttcgg cttgttgata ttgtccctga
    27961 cgttcgtaag cactggcgcg aatcaaactt gcttgtccac actgcggttt tttgttcagc
    28021 gcatgtgcac gtttcagcgc ctgactggca tacgccaccg cttgcgggaa atctgcgcgg
    28081 ttatattcca gcatcgccag cgccaggtta cagcgataat ccagcgggtc cagcgccacg
    28141 ccgcgcaggt agtaatcgaa cggtgaacgg ctggcgtgat gatattgctc cagatgctga
    28201 ccgataaacc aggcttcatc tgtactggta atgtcttgtg ctgccagtgg cgctttggcg
    28261 acgtccggca acggcaactc ctgcggctga tgttcctgat aactcagtac aatattgccg
    28321 tcggcatcgg agagttcaat ggtcagtctg tcaggattga taccgtgcaa cacgccctgg
    28381 atggcggtcg ctggtgtcag ggccacggca tcatcgagca acgcgttgca tttgccgatt
    28441 tcgcggatcg ccagacggta tccgttcaac ggagagatgg catacagccc ccactcaatc
    28501 ccccgctcac tacgctggag ttttatcacc gcatcgcggg aggcattttg caccatgccc
    28561 agagaatgat aaggcaggaa atactgctcg aaacgcttct cttcgtaagc atcaagccag
    28621 gtaaaatcag gctggttatc ggcaaaaata ccggtcatca gttcgatata cgggccgtta
    28681 ttgtcggtca gactcttatc ccacgcctgg ccaaattcac tgtgtcccca actccactgt
    28741 tttttacctg gcgcaatatg gtggttggca acgtgcagca aaccgccatc ttcatcgtga
    28801 caccacgcgc cgacaaaatc gtactgcgat ttttcggcca tatatgaggt tggaacaggc
    28861 acatttttat agcgagaaat gtccactccg gcggagtagt ccactttgta gtaagtgccg
    28921 gtggcgatgg ggaaagcgga gacggcccgt ttgccgtgat caaacaccgc agtcacatcc
    28981 ggtgggaaaa cgctctgatg cccttcaccc cctttcactg ccgggttgac ccaccacaag
    29041 aaatgacgcg gcgtggcgtt gccgttatag acgcggctgg cgatttccag tgccgcccgg
    29101 tcagggcgca gggtgaaacc tgtcatcacc tgtaaaccat gcatcggctc cgtttcgccg
    29161 acccacaccg tctgtgcacc gtcttcatgg gcttcgaggg tgaaatcaac gggcataaag
    29221 gtggtcgggc gatggtgttg cggccagtta aactcaatcc cgccggagat ccacggtccc
    29281 agcatcccca ccagcgcagg tttaatgact tcattgtgat agacaaaatc gcgctgtttc
    29341 actttatccc atgcgcgatg cacccgaccg cccagttccg gcaggatcat cactttgatg
    29401 tagtcgtttt ccagccacac cgcctgctag gatttcaggg ttttctgctc gctcagcgta
    29461 tcggtcacgc cgtagggata aaccgcgccg gacgatccct gataaacgcg attttccagg
    29521 aacatgggat gtatatcctg cggcccggtt tcataggtcg ggatctcaac gcgctcttgc
    29581 cacactttta ctggagtcat ggtgccctca atattaacaa gacatactga attaaaagat
    29641 ttgtggcagt gtattgaaca atctggcaat gttttcgcgg aataatcacg caattaacta
    29701 aacaaggttt agtgaagatg agagcctgca ttaacaatca acagattcgc caccataaca
    29761 aatgcgtgat tctggaactg ctgtaccggc aaaagcgcgc caataaatca acgctggccc
    29821 ggctggcgca aatttcgatt ccggcagtca gtaatatttt gcaggaactg gaaagcgaaa
    29881 aacgggtggt gaatattgac gatgaaagcc agacgcgcgg gcatagtagc ggtacatggc
    29941 tgattgcgcc ggaaggtgac tggacgctgt gcctgaacgt gacgcccacc agtattgagt
    30001 gtcaggttgc taatgcttgt ttaagtccga aaggcgaatt tgagtattta cagattgatg
    30061 taccgacgcc gcaggcgctg ctgtccgaaa tcgaaaaatg ctggcatcgc caccgtaaat
    30121 tgtggccgga ccgcaccatc aatctggcgc tggcaatcca cgggcaggtt gatccggtga
    30181 ccggcgtatc gcaaaccatg ccgcaagcgc cgtggacaac gccagttgag gtgaagtatc
    30241 tgctggaaga gaagctgggc attcgggtgc tggtcgataa tgactgcgtg atgctggcgc
    30301 tggcggagaa atggcaaaat aattcgcagg aacgggattt ctgcgtgatc aacgttgatt
    30361 acggcattgg ctcgtcgttc gtgattaacg agcaaattta tcgcggcagt ttgtatggta
    30421 gcggacagat tggtcacacc atcgttaatc cggatggagt cgtctgcgac tgtggacgtt
    30481 acggcagcct ggaaaccgtc gcctcgttaa gtgcattaaa aaaacaggcg cgggtatggc
    30541 taaaatcaca accggttaat actcagcttg agcctgaaaa actgacaacc gcgcagttaa
    30601 tcgctgcctg gcagagtgga gaaccgtgga tcaccagctg ggttgatcgc tctgccaatg
    30661 ccattggttt gagtctgtat aacttcctca acatcctcaa tattaatcag atttggttgt
    30721 acggtcgcag ttgtgccttt ggtgagaact ggcttaatac tattattcgc cagacaggat
    30781 ttaacccgtt cgaccgcgac gaaggaccga gcgtgaaagc gacgcaaatt ggcttcgggc
    30841 aattaagccg cgcacaacag gtgctgggaa ttggctattt gtatgttgag gcgcagttac
    30901 gacagatttg atggcgcgat aacgtagaaa ggcttcccga aggaagcctt gatgatcata
    30961 aacgaaaaat tgcctgatgc gctacgctta tcaggcctac acggagattg caatatattg
    31021 aatttgcaag attttgtagg cctgagaagg tgttcatgcc gcatccaaca tgaacaacga
    31081 gcacattgtc agcaaatcac cgtttcgctt atgcgtaaac cgggtaacgt gcgcagatgt
    31141 cgagaacttt acctttgatg cgctcgataa cggcttcatc attgatgctg tccagcacgt
    31201 cacacatcca gccagccagt tctttcgctt cggcttcttt aaagccgcga cgggtaatcg
    31261 ccggagtacc tacacgaata ccggaggtca caaacgggct cttcggatcg ttcggtacgc
    31321 tgtttttgtt gacggtgatg ttagcacggc ccagagcggc gtctgcttct ttaccggtca
    31381 ggtttttatc aaccagatca accaggaaca ggtggttatc agtgccgccg gaaaccactt
    31441 tgtagccgcg ctcgaggaac acttctacca tcgctttagc gtttttcgcg acctgctgct
    31501 ggtaagtttt gaactcaggc tccatcgctt ctttcagagc aaccgcttta ccggcgatta
    31561 cgtgcatcaa cggaccgccc tgaccaccag ggaaaacggc agagttcagt tttttgtaca
    31621 gctcttcgct accacctttc gccaagatca ggccgccgcg cggacccgcc agggttttgt
    31681 gagtggtggt agtaacaacg tgagcatgag gaaccgggtt cgggtagacg ccagcagcaa
    31741 ccaggcccgc aacgtgcgcc atatcaacga acagataagc accgatgctg tcagcgattt
    31801 cacgcatttt cgcccagtca accacgccgg aatatgcaga gaaaccaccg ataatcattt
    31861 tcggcttgtg ttctttggct tgtttttcca gatcggcgta atcgatatga ccggtagcat
    31921 cgataccgta aggaacgatg ttgtacagtt taccggagaa gttaaccgga gaaccgtgag
    31981 tcaggtgacc gccatgcgcc aggttcatac ccagaacggt atcacctggt tccagcagcg
    32041 cggtgtagac cgcaaagtta gcctgggagc cggagtgcgg ctggacgtta gcgtagtcag
    32101 cgccgaacag ttctttcgca cgatcgatcg ccagttgttc aacgatatca acatactcgc
    32161 aaccgccgta gtagcgtttg cccggataac cttcagcata tttgttggtc agctgagaac
    32221 cctgcgcctg cattacgcgc gggctggtgt agttttcgga ggcgatcagt tcgatgtgct
    32281 cttcctgacg tactttttcc tgctccatag cctgccacag ttcggcatca taatcggcaa
    32341 tgttcatttc acgctttaac atccgcatct cctgactcag ctaacaataa aatttttggc
    32401 ctttataggc ggtcctgttg gacaacggcg aacagtataa ccgaatcatt gtgcgataac
    32461 aggtcttgac aaaggaattt acgcaaacga ttaccttcag gctacgcaag gctttggaga
    32521 ataaagagct tgcaaccgga aacggatttc ttttcaggtt tgtgatgcaa atttttcact
    32581 tcatcacatt ctttctgaaa aacaccaaag aaccatttac attgcagggc tattttttat
    32641 aagatgcatt tgagatacat caattaagat gcaaaaaagg aagaccatat gcttgacgct
    32701 caaaccatcg ctacagtaaa agccaccatc cctttactgg tggaaacggg gccaaagtta
    32761 accgcccatt tctacgaccg tatgtttact cataacccag aactcaaaga aatttttaac
    32821 atgagtaacc agcgtaatgg cgatcaacgt gaagccctgt ttaacgctat tgccgcctac
    32881 gccagtaata ttgaaaacct gcctgcgctg ctgccagcgg tagaaaaaat cgcgcagaag
    32941 cacaccagct tccagatcaa accggaacag tacaacatcg tcggtgaaca cctgttggca
    33001 acgctggacg aaatgttcag cccggggcag gaagtgctgg acgcgtgggg taaagcctat
    33061 ggtgtactgg ctaatgtatt tatcaatcgc gaggcggaaa tctataacga aaacgccagc
    33121 aaagccggtg gttgggaagg tactcgcgat ttccgcattg tggctaaaac accgcgcagc
    33181 gcgcttatca ccagcttcga actggagccg gtcgacggtg gcgcagtggc agaataccgt
    33241 ccggggcaat atctcggcgt ctggctgaag ccggaaggtt tcccgcatca ggaaattcgt
    33301 cagtactctt tgactcgcaa accggatggc aaaggctatc gtatcgcggt gaaacgcgaa
    33361 gagggtgggc aggtatccaa ctggttgcac aatcacgcca atgttggcga tgtcgtgaaa
    33421 ctggtcgctc cggcaggtga tttctttatg gctgtcgcag atgacacacc agtgacgtta
    33481 atctctaccg gtgttggtca aacgccaatg ctggcaatgc tcgacacgct ggcaaaagca
    33541 ggccacacag cacaagtgaa ctggttccat gcggcagaaa atggcgaagt tcacgccttt
    33601 gctgatgaag ttaaggaact ggggcagtca ctgccgcgct ttaccgcgca cacctggtat
    33661 cgtcagccga gcgaagccga tcgcgctaaa ggtcagtttg atagcgaagg tctgatggat
    33721 ttgagcaaac tggaaggtgc gttcagcgat ccgacaatgc agttctatct ctgcggcccg
    33781 gttggcttca tgcagtttac cgcgaaacag ttagtggatc tgggcgtgaa gcaggaaaac
    33841 attcattacg aatgctttgg cccgcataag gtgctgtaat ttgatgttgc cggatggaaa
    33901 catccggcaa cccttgacgc ggtttaaatt gccgcgtcgt cctcttcacc ggtacggatg
    33961 cgaatgaccc gagccacgtc aaagacgaag attttaccgt caccgatttt gccggtttgc
    34021 gccgtgcgaa taatggtatc gacacaggta tcgacaatgt cgtccggtac gacaatctca
    34081 attttcactt tcggcagaaa atccaccata tactccgcgc cgcggtacag ctcggtatgg
    34141 cctttctggc gaccaaagcc tttcacttcg gtcaccgtca tgccggtaat accgacttcg
    34201 gccagtgctt cgcggacatc gtccagcttg aagggtttta taatcgcatc aatctttttc
    34261 atgctattcc ttgaaaaggt tgcctgtctt ttgatctgct aaacgtaaca cataacgcca
    34321 attcattcct tgaaatcgtt tgcatccagc tcgtgtcggg aaagcagttt ataaaattct
    34381 gtccggttgc gccccgccat tctcgccgcg tgggtgacgt tgcctttggt gatttgcagc
    34441 agcttacgca agtagttgag ttcaaaatga ttacgtgcct caacaaaggt tggcagcgcc
    34501 gtattttcac cctccagcgc ctgctccacc agcgcatcac taatcaccgg agatgaggtc
    34561 agcgccacgc actgttcaat cacgttgacc aactggcgca cattacccgg ccagctcgcg
    34621 gtcatcaggc gtttcatcgc atcggtagag aacgcgcgga caaacggttt atgtcgctct
    34681 gccgcctggc gcaacaggtg atttgccagt agcggaatgt cttctgtgcg ctccgccagt
    34741 gccggaattt tcaggctgac aacgttgagg cggtaataga gatcttcgcg gaattccccg
    34801 cgcgccatcg cttttgacag atcacggtga gtggcagaaa taatccgcac attgatatca
    34861 atatcgcggt tactgcccag cgggcgcact ttacgctcct gcaacacgcg cagcagtttg
    34921 acctgtaacg gcgcaggcat atcgccaatt tcatcgagaa atagcgttcc gccttccgcc
    34981 gcctggaata aaccttcgcg attgctgaca gcgccagtaa acgcgccacg cgcatgacca
    35041 aacagctccg actccagcaa ttgctcgggc aatgcgccac agttaatagc aataaatggt
    35101 ttgctgttgc gcgggctggc gttgtggata gcctgggcga aaatctcttt cccggtgccg
    35161 ctctgaccgt taatcaaaac gctgacgtct gattgcgcca ccagccgcgc ctgttccagc
    35221 aaacgcagca tcagcgggct gcgggtgaca attgcctcgc gccagcgttc atcggtggct
    35281 ggcgcggatt gctccagcgc atcgtcaatt gcctgatata gcgcatcttt atcgacaggc
    35341 ttggtgagga aactaaaaac gccctgctgt gttgcagcaa cggcatcggg gatagaacca
    35401 tgcgcagtaa gaataattac tggcattccc ggctgcactt tctggatttc agcaaatagc
    35461 tgcataccgt ccatttcatc catccgcagg tcgctgataa ctaaatctac tttttcgcga
    35521 ttcagtaccc gtaatccttc agcgccactt tccgccgtga ccacactgta gccttcgctg
    35581 gtcaggcgca ggccaagcag tttcagcaat cccggatcgt catcgaccaa taataaatgc
    35641 gcaggtttat ggctcatcag gagtgacctc atgggtggat ggcgcgggct tttcactctc
    35701 gtgcggcgta tccggcgaga aatttccagc cggtttgcgg gtcgagagct ggcgttcaat
    35761 atcggtcagg ttttccagct tgcgggtggt gagttccagt tgctgctgta gaacgtgatg
    35821 ttgctggcgc aatgtatcca gctcgctgtc gctggactgc tggagtttac tgtaacgttg
    35881 gcgctcttcc gccagttgca gttgcagcgc ctgaccgtcg cgccagagtt gatacagtgg
    35941 gcgaacctgt gccgggatct cggtacttaa cgcttcaata cgcgcgacca gctggcggcg
    36001 ctcatacggc gtaattttgg cgtcggcgag caaaatccct tgtttaaagg tattttgcca
    36061 gctgccgtcg tcatattggc gagcttgctg acgcgactgc gcaggcatta aacgatcagc
    36121 acaatccatc gcccgcagcc agtaaagcgg attggtttcg gttgatttac cttgcagcgc
    36181 ccagatgtcg ctacattcag tagaaagata gtcagccagt tgataaaccg gaattttttc
    36241 ttctgctggc gtatcaatgg ctggcttatt gtgattctgc acgcaaccca gcaatgccag
    36301 acatggcagc cctgccagcc acagccgtcg gggcaataat cgttgaaaaa tgtgtcgcat
    36361 attcaccaga cttaaagcct atcccagtgg gcgtaattgt tgcagacagt ctggacatgg
    36421 actgcgcgga gaaaccgggg cgtacatatc gtacgtgaag atttcgagca ctacccgggg
    36481 ccgaactgac aaataaaata gcctgatagg ataggctgtt agcattattt cgtgtttttc
    36541 gacgacggta attcaatgcg gaaacaaacg tcttgcccgc tctcgtcgac cagatacagt
    36601 tccccttgca tacggcgaat acaatccctg gcaatgctta atcccagacc gctgcccttc
    36661 accgcccctt ttcgctggtg gcttccctga aaaaagggtt cgaagatcat ggcgcgttcc
    36721 tcttgcggaa tgggcgtgcc tgtattgatg acatcaatat aaacccgcgc accatgtaaa
    36781 ctgctgcgaa ggcaaatgtt accggattca gccccgtagt gcaccgcatt ggagtaaaga
    36841 ttatccagta cgctcatcag cagcattggc tccgccaggc aagctgttgc tttgagatcg
    36901 acgtcggtat gcatcatttt agcccgtgcg ggcaggctat gagcagaaac cactgtctcc
    36961 accagcggtg ctaattcaac attctccagt tccaccgcac tgtccgcctg tttacggttg
    37021 taatcaagca gttgttcgat cagtttttgc aaattgcagc tgctgctatc aagaatgctc
    37081 accacctctt tttgctctgg cgtaagcggc ccgacaacct gatcagccag taattcagtg
    37141 ccctcgcgca tactcgccag tggcgttttt aattcatgag ataaatgtct taaaaattga
    37201 tggcgttggg attccagcca tgacaggcgc tcacttaacc aaagaatacg ttgcccaacc
    37261 gagcgtaact cgctcggtcc acagaacgaa acgctattgc ccagagaacg cccttccccc
    37321 agacggttga tcatgcgctc gatatttttc accggcccga taatcatccg cgtgaaaagt
    37381 agcaccatca ccagactcac cagaaatagc accagcgatt gccaaccaaa atattgccca
    37441 cgttcggcga tttcacgctg aagttgctgc ccacgagaga acaccactgt gcgcgtggcc
    37501 tgcaccattt cggtattggc actggcaaag gcttcaagac gcgcggcggc ggcagcatcg
    37561 ggaccactgt tgttacactg aagttgggcc agattgttca aatcctgacg caatgcctgg
    37621 taaagtttat cgtctggcag tacgcctgcg tgggcatcga gcatttcgct gtaacgcttg
    37681 cgctggcttt gataaacctt cgcaagcgta gggtcgtcca gcacgcaata ctgacggtag
    37741 ctacgctcca tctccagcgc cgcgttggtc atcgcttcac tacgccgggc atcaataagc
    37801 gtagtgcggt taaccagcgc cgcctgatcg ctaagcgcat tcaggctttg ccaggcttgc
    37861 catgccagca ccaacagggg tagtagaatt agcaaaaatg ccagcattac cagttgtcgt
    37921 aatgagcggg gaaaaacggg ccagcgtttc aaggtgttac tctcgtcaga cgcgaatagc
    37981 ctgatgctaa ccgaggggaa gttcagatac aacaaagccg ggaattaccc ggctttgtta
    38041 tggaataagg cggtgcctaa ctcgacgttt cgcccgatgg ttgatatagc tacgctgata
    38101 tcagaagttg gacggcaggc accttgttgt gcgtcattcg aattttatgt agcacgtccc
    38161 gaaggggctg acataagtcg gtgaatgagc cactgcttac tattatgcag taactgtgcc
    38221 aataaagaaa atagtttggt aacgtactga ttatacgttg ctttgaggga tttatggctt
    38281 cttcgctatt tgaatgtaaa tcagccactg tgtcgctaaa aagagacaac ttaagataaa
    38341 cttattagat tgcaataaaa tcaatcattt aagtgtcgcc agaaagcgac acggtaaacc
    38401 acccattgtc gtgatttaca gacacaaaaa agctcccgga gttgggagct tatgatagtg
    38461 gttggtgctt aatgccgcat ccgggctgca aaaccaatga actgatgact taccccaact
    38521 gcttacgcgc attgcggaaa atgcgcatcc atgggccatc ctcgccccag ttttccggat
    38581 gccaggagtt gctgacagta cggaaaacac gttccgggtg cggcatcata atggtgactc
    38641 gaccactttc agtcgtaacc gccgtaatac cgttcggtga accgttcggg ttagccgggt
    38701 aggtttcagt gactttgccg aagttatcga catagcgcag tgccaccagc cctttgcttt
    38761 ccagtgccgc cagatgcgcc gcatcacgca cttccacgcg cccttcacca tgagagacag
    38821 caatcggcat ttgcgagccc accatcccct gcaacagcag agacgggctt tgggttactt
    38881 caaccaggct gaaacgcgct tcaaagcgat cggaggtgtt acgcacaaaa cgtggccaca
    38941 actcactccc cgggatcagt tcacgcaggt tagacatcat ctggcaaccg ttacataccc
    39001 ccagggccag cgtttgcgga cgatggaaga aggttgcaaa ctcatcgcgt acacggtcat
    39061 tgaacaggat agacttcgcc caaccttcac cggcacccag cacatcaccg taggagaaac
    39121 ctccgcacgc gaccagggcg tgaaaatcct ccagtcccgt acgtccagtc agcaggtcgc
    39181 tcatatgcac gtcgatagca tcaaagcccc cacgttggaa agctgccgcc atttcaacat
    39241 gcgagttcac gccctgctca cgcagtacgg caactttcgg acgtgcgcca gtggcaatat
    39301 acggtgctgc cacatcttcg ttgatatcga acgacagttt tacattcagg cccggatcgg
    39361 cgtcgttaga tttcgcctga tgctcctgat cggcacactc cgggttgtca cgcaggcgct
    39421 gcatctgcca ggtagtttct gcccaccaga cacgcaacgt agtgcggctt tcgctgaata
    39481 cagtctgccc gttggcggta atcacaaaac ggtcaccgga aaccgcctgc ccaacataat
    39541 ggacgcaatc cgcaagccca tgctgtgcca gtacggactc gaccgcttca cggtcagcgg
    39601 cacgaacctg aatcaccgca cccagttctt cgttaaacaa cgccgccagg cgatcgtcac
    39661 ccagagaggc gatatccgcg tcaatgccac aatgacctgc aaaggccatt tccgccagcg
    39721 ttaccagcag gcctccatca gagcggtcgt gatacgccag cagcttacgc tgtgcaacca
    39781 gcgcctgaat cgcgtcatag aagcctttca gttggacaac atcgcgtaca tctgccggtt
    39841 tgtcgccaag ctgacgataa acctgcgcca gcgccgttgc gcccagcgcg ttattgcctt
    39901 tgcccaaatc aatcagcagc agtgcgttat cttcggtaca aagctgcggc gtgatggtgt
    39961 gacgtacatc ttccacgcgg gcaaatgcag aaatcaccag cgacagcggc gacgtcattt
    40021 cgcgctcttc gttaccttcc tgccagcggg ttttcatcga catagagtct ttacccaccg
    40081 ggatcgtcag gcccagcgcc ggacaaagtt cttcacccac ggctttaacg gcttcataca
    40141 ggcccgcatc ttcaccaggg tggcctgccg ccgccatcca gttggcggaa agtttaatgc
    40201 gtttgatatc gccaatctgt gttgcggcaa tattggttaa cgcttcaccg accgccagac
    40261 gggcagaggc ggcgaaatcc agcagcgcaa ccggcgcacg ctcgccaatc gccatcgctt
    40321 caccgtagta gctgtcgagg ctggcggtag tgaccgcgca gttagcgacc ggcacctgcc
    40381 acggccccac catctgatcg cgcgccacca tgccggttac gctgcggtca ccgatggtca
    40441 ccaggaaagt tttctccgcc acggtcggca gatgcagtac gcgtttcacc gcgtctgcaa
    40501 tggtgatccc ttcacggacc agcgcatcgc ccttcgcttt cagcgtttgt acatcgcggg
    40561 tcatcttcgg cgttttacca agcaggacgt ccagcggcag atcgatcggc tgattatcaa
    40621 aatgacgatc gtgcagagaa agatgcagtt cttcggtcgc ttcaccaatc accgcgtagg
    40681 gtgcgcgctc acgcttacac agttcgtcaa acagcggtaa ttgatcggca gcaaccgcca
    40741 gcacgtagcg ttcctgggat tcgttacacc agatttccag cgggctcatg cccggttcgt
    40801 cgcttagaat atcgcgcagt tcaaatttac cgccgcgtcc gccgtcgctc accagctccg
    40861 gcatcgcgtt agaaagaccg cccgcaccga cgtcgtggat aaacagaatt gggttagcat
    40921 caccaagctg ccagcaacgg tcgatcactt cctggcaacg acgctccatc tccgggttgt
    40981 cgcgctgtac ggaagcaaag tcgaggtcgg catcagactg accagacgcc atggaagacg
    41041 ccgcgccgcc gccaagaccg atgttcattg ccgggccgcc gagaacgacc agcttcgcac
    41101 cgacgttgat ctcgcctttt tgtacgtgat cggcgcgaat gttgccgatc ccgcccgcca
    41161 gcatgatcgg tttgtgataa ccgcgcagct cttcgccatt gtggctgttc actttttctt
    41221 cataagtacg gaagtagccg ttcagtgccg gacgaccaaa ttcgttgtta aacgccgcac
    41281 cgcccagcgg gccttcggtc atgatgtcca gcgcggtaac aatgcgctca ggcttaccga
    41341 aatcttcttc ccacggctgt tcgaagccag gaattcgcag gttggatacg gagaaaccaa
    41401 ccaaaccagc tttcggcttc gcgccgcgcc cggtggcacc ttcatcgcgg atttcaccgc
    41461 cggaaccggt cgccgcgccc ggccacggag aaatcgccgt cgggtggttg tgagtttcga
    41521 ctttcatcag gatgtgcgcc ggttcctgat ggaaattgta gcggcccgtt tcgtggtcag
    41581 caaagtagcg gcccacttca gaaccttcca ttacggcggc gttatcttta taagcagaga
    41641 gaacgtgatc tggcgtggtt tcgaaagtat ttttgatcat cctgaacagc gatttcggct
    41701 gttgttcacc gtcgataatc cagtcggcgt taaaaatttt gtggcggcag tgctcagagt
    41761 tcgcctgagc gaacatatac agctcgatgt cgttcgggtt acgaccaagc tttgtgaaag
    41821 catcctgcag atagtcaatt tcatcttccg ccagagccaa gccaagacgc aggttagcgt
    41881 cgatcagcgc ctgacggccc tgccccagca aatcaacgct tgtgaccgga gtcggttgat
    41941 ggtgggcaaa caactgctct gcatcatcta aatcaaaaaa gaccgtttcc atcatgcggt
    42001 cgtgcagttc agcggtaacc tgctgccatt gttcattggt cagcgtaccg gcttctatat
    42061 agtaagcaac gccgcgctca aggcggttta cctgttgtag cccgcagtta tgggcaatat
    42121 cggtcgcttt cgaagaccag ggagagatgg tgccaggacg cggggtcacc agcaggagtt
    42181 tgccttgcgg ggcgtggctg gcgagtgccg gaccatattt cagcaggcgt tcaagttgtg
    42241 cgtgctcatc atcgtttaac ggcgcattga ggtcagcaaa atggacatac tcggcgtaaa
    42301 tattgtgaac cgggagcctg gcagcctgaa aacgtgccag cagtttgttg attcggaatg
    42361 ccgacagtgc aggcgaacca cgcagaattt ccatcataag tctctcgtct tctaagcttt
    42421 cggtgtaccc aagggggggg aaacgggcgt cattataaag aatctgatgc gctgacgaaa
    42481 ccgtttgcgt ggaaataaaa tcaccatcgt gaattagcaa cgcgtgccgc caatggctgt
    42541 aataagttgc catctggcgc aggtttacgc aaaatgccgc tcatttatga gtaaaccttt
    42601 cactattatt acgttttttc aagctgggac gcgcacgaca cagagaatta actaattgaa
    42661 aaaattaaag attaattatc tgttcatcgg cattctggca ctgctgctcg cggtcgctct
    42721 ctggccatcc attccctggt ttggtaaagc cgacaaccgt atcgccgcca ttcaagcgcg
    42781 gggagagttg cgtgtgagca ccattcatac tcccctgact tataacgaaa tcaacgggaa
    42841 accttttggc ctggattacg aactggcgaa acagtttgcc gattacctcg gcgtaaaact
    42901 gaaagtgacc gtgcggcaga atatcagcca gctgtttgac gaccttgata atggtaacgc
    42961 cgacctgctg gcggcaggac tggtctataa cagtgagcgg gtaaaaaatt atcagcctgg
    43021 ccctacctat tattccgtgt cacaacaact ggtttataaa gtgggtcagt atcgcccacg
    43081 tacgctgggc aacctgacgg cggagcaact caccgttgca ccgggtcatg tggtagttaa
    43141 cgatctccag accctgaaag aaacaaaatt cccggaatta agctggaagg tagacgacaa
    43201 aaaaggctct gcggaattaa tggaagatgt catcgaagga aaactcgatt acaccattgc
    43261 tgattctgtc gccatcagcc tgtttcagcg cgttcacccg gagctcgccg tagcgctcga
    43321 tatcaccgat gaacaaccgg tgacttggtt tagcccgtta gatggcgata ataccctttc
    43381 cgccgccctg ctcgacttct tcaacgaaat gaatgaagac ggtacgctgg cacgcattga
    43441 agagaaatac ctggggcatg gcgatgattt tgattacgtc gatacgcgca catttttacg
    43501 cgccgtcgat gcggtactgc cgcagttaaa gcccctgttt gagaaatacg ccgaagaaat
    43561 tgactggcgt ttgctggccg ctattgctta tcaggaatcg cactgggatg cacaggccac
    43621 ttcaccgacg ggtgtgcgcg gcatgatgat gttaaccaaa aataccgcgc aaagcctcgg
    43681 cattacggat cgtaccgatg ccgaacagag catcagcggt ggcgtgcgtt atttgcagga
    43741 tatgatgagt aaagtgccgg aaagtgtgcc ggagaacgaa cggatctggt ttgccctcgc
    43801 tgcgtacaat atgggctatg cgcatatgct ggatgcccgc gccctgacgg caaaaaccaa
    43861 agggaatcct gacagttggg ctgacgtaaa acagcgtctg cctttactta gccagaaacc
    43921 ctattacagc aagctgactt acggctacgc tcgtgggcat gaagcctacg cttatgtcga
    43981 aaatattcgt aagtatcaga ttagcctggt gggttatctg caagagaaag agaagcaggc
    44041 tacagaagcg gcgatgcaac tggcgcagga ttatccggcg gtatcgccta cggagttggg
    44101 caaagagaaa tttccttttc tctcgtttct ttcccagtcg tcatcaaact atttgaccca
    44161 ttctccctct ctgctgtttt ccaggaaagg gagtgaagag aaacaaaatt aatccgtcga
    44221 ggattgcgct tttttctgcg ctttaatttc ctggcggcgc atgcgaaaga agtcactgag
    44281 caacgccgcg cactcatccg ccagtattcc ttccgtaatt tccactcggt gattcatacc
    44341 cggatgatgc agcacatcca ttaaagatcc cgcagcgcca gttttcgcgt cacgcgcacc
    44401 aaagaccacg cgaccaatgc gactgtggat catcgctccg gcacacatta cacatggttc
    44461 aagcgtgaca tacaacgtgg cgtcgatcag acgataattt tgcatcacca gaccaccctg
    44521 ccgcagggcc atgatttctg catgtgcggt gggatcatgg cgaccaatcg ggcggttcca
    44581 gccttcgccg attacccgat tgttatgcac taataccgcg ccgaccggca cttcccgctc
    44641 atcccaggca cgtttcgcca gcgtcagcgc gtgacgcatc caatattcgt ggctaaattc
    44701 gacttcagac aaaaagaaaa ctccggttat aaaagcgcgg cgcattatac acggacgcta
    44761 tgctttactc tattccagtt gctggagttc accgcgcggg gttactcgcc aacgatgctg
    44821 acagaaataa agcagcggat tgtcctgatt actgtcgcta tagccactgt acagccgcag
    44881 cggcgtgccg attttgcgct ccagttgtgc gaccttttca tgtcccagac aacgcatcgt
    44941 caatacccaa ccaccatagc cacgctgaat ttggctggcg ataagattaa cccgcggcag
    45001 ccagggcgta tcgaaataaa ccgcttcaac cagcggctgc ggagagccgg taatcaacca
    45061 gatatcagca tcggaactta acaggtaggt ggttaatcgc tcctgaacca gcggaaaggc
    45121 ggtaacattg tcgcgaaacc agcgcacgaa atcggcctgc aacgtctgta aacgtgcttc
    45181 gctgtgacca aaagtgcacc cccacagaag cagactcatc ggccagcgtg ccgcacgacc
    45241 ttttatcaat aacgcaatgg ctataatcgg taacaacggc aggacaagta acgcattcag
    45301 cggttggcga cgcagtaaat agcgcagaaa actgccgaac atatcctgct gatgcaatgt
    45361 tccatctaag tcaaaaaaca ccacacgacg ctcgtgagtt gccaaaccat actcctctgg
    45421 actgaaacat cctgattaat tttcgctact acatagccta acagatagat catcactttt
    45481 ccggcaacaa tcgaccgcat catggctaac tggaattttt aattcatggc aattagcggc
    45541 aatggaatat aaaattcact cgcgtgtgtc tcatattgcc tgttgtcgcc actatttcat
    45601 cgcgaggaaa gatatgaacg gcttacttcg tatccgtcag cgttaccaag ggcttgccca
    45661 gagtgataaa aaactggcgg attatctgct gctacaacct gatacggcgc gccatttaag
    45721 ctctcagcaa ctggccaacg aagccggagt cagtcagtcc agcgtcgtga agttcgcgca
    45781 aaaactcggc tataaaggtt ttccggcgct taagttggcg ttgagtgaag cgctggcaag
    45841 ccagccggaa tcgccctccg tgcccattca taaccaaatc cgcggtgatg atccgttacg
    45901 gctggtcggc gaaaaactga ttaaagaaaa taccgccgct atgtacgcaa cgctaaacgt
    45961 taatagtgaa gagaaactgc atgaatgcgt aacaatgttg cgctctgcgc ggcggataat
    46021 tctgaccggt attggcgctt cgggtctggt ggcgcaaaac tttgcctgga agctgatgaa
    46081 gattggcttc aatgctgccg cactgcgcga tatgcatgcg ctactcgcaa cagtacaggc
    46141 gtcgtcccct gacgatctgt tattagccat ttcctacacc ggtgtacgac gcgagttaaa
    46201 cctggcggca gatgagatgc tgcgagtggg cggaaaagtg ctggcgatta ccggctttac
    46261 tccgaatgcc ctgcaacagc gtgcttctca ttgcctgtat accattgccg aagaacaagc
    46321 gacaaacagt gcttcaatct ctgcttgtca cgctcaggga atgttaacgg atttgctgtt
    46381 cattgcgctg attcagcagg atctggaact ggcaccagaa cgtattcgtc atagtgaagc
    46441 gctggtgaaa aaactggtct gagtaaagaa tgcgcgtata atgcccgccc ggtttgtgtt
    46501 gttttgagag tttccttatg gcgctgttaa tcactaaaaa atgcatcaat tgtgatatgt
    46561 gtgaaatcaa ataaaaacac atttattttc atagtgatac agcaatgtgt tattgaatgt
    46621 gttgttggat ttatggtgca aaaaaatttt gcataagttc agcaacacaa agaacaccac
    46681 tttctttaac gtcctccgcg ccctaaagga cggggcttta cggcgcaaaa ggcctgtgac
    46741 atacaacatt aacttttcag tcaaccgtag cacgttcttg tatacgccgt gtctgcggtt
    46801 ttatataacc ctggctggaa acctcttata caaagtcgat tcgccaacat catagatgat
    46861 cgccaccttc tggcgagaaa ctcctgatgc aattagtcgc ccggcctgcg cccattattc
    46921 tggtgaaagt ttgggacggc gaccgccaat tcgtccctgt gcgcgagcag cttccagtcc
    46981 tgcttttgtt cgttcaacaa tcagttcacg ttccatttca gccagggcac ccatcacatg
    47041 gaagaaaaag cgtcccattg gtgtgctggt atcaattgaa tcagtcagac tacgaaagtt
    47101 gatgcctcgt tcgcgcaacg cctccaccag cacgacaaga tgccgcatac tgcgccccag
    47161 ccgatccagc ttccagacaa ccagtgtgtc gcctgccgat aatgtcttga gcagtttttt
    47221 cagccccggt ctttcggact ttgtaccact tatcttgtct tcaaaaatca gctcgcatcc
    47281 tgcacagttc aacgcattac gttgtagatc ggtattctgg tcatttgttg ata
//
LOCUS       DAJTUI010000024        45629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTUI010000024 DAJTUI010000000
VERSION     DAJTUI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.024"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..205
                     /locus_tag="OO464_001434"
                     /pseudo
     CDS             1..205
                     /locus_tag="OO464_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094514.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            404..2092
                     /locus_tag="OO464_001435"
     CDS             404..2092
                     /locus_tag="OO464_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6226636.1"
                     /translation="MQSPLPYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNL
                     SDSKFISSFKSWFESGKIKNIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGE
                     FITYYENGAIESKRHFNNDKIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGK
                     LMQENFYQQSVLIGNDTSYYQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKD
                     GKQISVQTWYENGQKEEEKHFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEW
                     YENGIPESLYPYKNGKTDGIAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKE
                     TLYVNGIRQGVEKEFNDRTGKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLS
                     SLDNPTQIKDNATTGDSSAQFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATA
                     YKGNGIPANNEKYLAYLQQAATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAA
                     QGNFAAHFYLGRMYQNGDGVERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2145..3875
                     /locus_tag="OO464_001436"
     CDS             2145..3875
                     /locus_tag="OO464_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226637.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(3935..4426)
                     /gene="yajQ"
                     /locus_tag="OO464_001437"
     CDS             complement(3935..4426)
                     /gene="yajQ"
                     /locus_tag="OO464_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6226638.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4594..5505
                     /gene="panE"
                     /locus_tag="OO464_001438"
     CDS             4594..5505
                     /gene="panE"
                     /locus_tag="OO464_001438"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6226639.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5468..6058
                     /gene="yajL"
                     /locus_tag="OO464_001439"
     CDS             5468..6058
                     /gene="yajL"
                     /locus_tag="OO464_001439"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6226640.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6112..7560)
                     /gene="thiI"
                     /locus_tag="OO464_001440"
     CDS             complement(6112..7560)
                     /gene="thiI"
                     /locus_tag="OO464_001440"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6226641.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7766..8008
                     /gene="xseB"
                     /locus_tag="OO464_001441"
     CDS             7766..8008
                     /gene="xseB"
                     /locus_tag="OO464_001441"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6226642.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8008..8907
                     /gene="ispA"
                     /locus_tag="OO464_001442"
     CDS             8008..8907
                     /gene="ispA"
                     /locus_tag="OO464_001442"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6226643.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            8932..10794
                     /gene="dxs"
                     /locus_tag="OO464_001443"
     CDS             8932..10794
                     /gene="dxs"
                     /locus_tag="OO464_001443"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6226644.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            10849..11766
                     /gene="yajO"
                     /locus_tag="OO464_001444"
     CDS             10849..11766
                     /gene="yajO"
                     /locus_tag="OO464_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6226645.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(11877..12395)
                     /gene="pgpA"
                     /locus_tag="OO464_001445"
     CDS             complement(11877..12395)
                     /gene="pgpA"
                     /locus_tag="OO464_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6226646.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12373..13350)
                     /gene="thiL"
                     /locus_tag="OO464_001446"
     CDS             complement(12373..13350)
                     /gene="thiL"
                     /locus_tag="OO464_001446"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6226647.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13428..13847)
                     /gene="nusB"
                     /locus_tag="OO464_001447"
     CDS             complement(13428..13847)
                     /gene="nusB"
                     /locus_tag="OO464_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6226648.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(13867..14337)
                     /gene="ribE"
                     /locus_tag="OO464_001448"
     CDS             complement(13867..14337)
                     /gene="ribE"
                     /locus_tag="OO464_001448"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6226649.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14426..15529)
                     /gene="ribD"
                     /locus_tag="OO464_001449"
     CDS             complement(14426..15529)
                     /gene="ribD"
                     /locus_tag="OO464_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR6226650.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15533..15982)
                     /gene="nrdR"
                     /locus_tag="OO464_001450"
     CDS             complement(15533..15982)
                     /gene="nrdR"
                     /locus_tag="OO464_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6226651.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16133..16672
                     /locus_tag="OO464_001451"
     CDS             16133..16672
                     /locus_tag="OO464_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6226652.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            16970..17854
                     /gene="tsx"
                     /locus_tag="OO464_001452"
     CDS             16970..17854
                     /gene="tsx"
                     /locus_tag="OO464_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6226653.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18031..18378)
                     /locus_tag="OO464_001453"
     CDS             complement(18031..18378)
                     /locus_tag="OO464_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6226654.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18507..19478)
                     /gene="secF"
                     /locus_tag="OO464_001454"
     CDS             complement(18507..19478)
                     /gene="secF"
                     /locus_tag="OO464_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6226655.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19489..21336)
                     /gene="secD"
                     /locus_tag="OO464_001455"
     CDS             complement(19489..21336)
                     /gene="secD"
                     /locus_tag="OO464_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6226656.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21364..21696)
                     /gene="yajC"
                     /locus_tag="OO464_001456"
     CDS             complement(21364..21696)
                     /gene="yajC"
                     /locus_tag="OO464_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6226657.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21719..22846)
                     /gene="tgt"
                     /locus_tag="OO464_001457"
     CDS             complement(21719..22846)
                     /gene="tgt"
                     /locus_tag="OO464_001457"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6226658.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(22901..23971)
                     /gene="queA"
                     /locus_tag="OO464_001458"
     CDS             complement(22901..23971)
                     /gene="queA"
                     /locus_tag="OO464_001458"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6226659.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24064..24637
                     /gene="acpH"
                     /locus_tag="OO464_001459"
                     /pseudo
     CDS             24064..24637
                     /gene="acpH"
                     /locus_tag="OO464_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24642..26459)
                     /gene="malZ"
                     /locus_tag="OO464_001460"
     CDS             complement(24642..26459)
                     /gene="malZ"
                     /locus_tag="OO464_001460"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR6226660.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26615..27988)
                     /gene="proY"
                     /locus_tag="OO464_001461"
     CDS             complement(26615..27988)
                     /gene="proY"
                     /locus_tag="OO464_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6226661.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28064..29383)
                     /gene="brnQ"
                     /locus_tag="OO464_001462"
     CDS             complement(28064..29383)
                     /gene="brnQ"
                     /locus_tag="OO464_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6226662.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29790..31085)
                     /gene="phoR"
                     /locus_tag="OO464_001463"
     CDS             complement(29790..31085)
                     /gene="phoR"
                     /locus_tag="OO464_001463"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6226663.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31143..31832)
                     /gene="phoB"
                     /locus_tag="OO464_001464"
     CDS             complement(31143..31832)
                     /gene="phoB"
                     /locus_tag="OO464_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR6226664.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32022..33224
                     /gene="sbcD"
                     /locus_tag="OO464_001465"
     CDS             32022..33224
                     /gene="sbcD"
                     /locus_tag="OO464_001465"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6226665.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33221..36364
                     /gene="sbcC"
                     /locus_tag="OO464_001466"
     CDS             33221..36364
                     /gene="sbcC"
                     /locus_tag="OO464_001466"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6226666.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36490..37671
                     /gene="araJ"
                     /locus_tag="OO464_001467"
     CDS             36490..37671
                     /gene="araJ"
                     /locus_tag="OO464_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6226667.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37814..38722)
                     /gene="mak"
                     /locus_tag="OO464_001468"
     CDS             complement(37814..38722)
                     /gene="mak"
                     /locus_tag="OO464_001468"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR6226668.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            38847..39758
                     /gene="rdgC"
                     /locus_tag="OO464_001469"
     CDS             38847..39758
                     /gene="rdgC"
                     /locus_tag="OO464_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6226669.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39836..40291)
                     /locus_tag="OO464_001470"
     CDS             complement(39836..40291)
                     /locus_tag="OO464_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226670.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40288..40623)
                     /locus_tag="OO464_001471"
                     /pseudo
     CDS             complement(40288..40623)
                     /locus_tag="OO464_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40778..41062)
                     /gene="ppnP"
                     /locus_tag="OO464_001472"
     CDS             complement(40778..41062)
                     /gene="ppnP"
                     /locus_tag="OO464_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR6226671.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41134..41811)
                     /locus_tag="OO464_001473"
     CDS             complement(41134..41811)
                     /locus_tag="OO464_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6226672.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42069..42260)
                     /gene="yaiA"
                     /locus_tag="OO464_001474"
     CDS             complement(42069..42260)
                     /gene="yaiA"
                     /locus_tag="OO464_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6226673.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42310..42834)
                     /gene="aroL"
                     /locus_tag="OO464_001475"
     CDS             complement(42310..42834)
                     /gene="aroL"
                     /locus_tag="OO464_001475"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6226674.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43017..43475)
                     /locus_tag="OO464_001476"
     CDS             complement(43017..43475)
                     /locus_tag="OO464_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6226675.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43595..44404
                     /gene="proC"
                     /locus_tag="OO464_001477"
     CDS             43595..44404
                     /gene="proC"
                     /locus_tag="OO464_001477"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6226676.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44421..45536)
                     /gene="adrA"
                     /locus_tag="OO464_001478"
                     /gene_synonym="dgcC"
     CDS             complement(44421..45536)
                     /gene="adrA"
                     /locus_tag="OO464_001478"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR6226677.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11841 a        11919 c        11183 g        10686 t
ORIGIN      
        1 cgaatcccct cccgcaagca ctataacttt cattgcacaa atcgatcatg ttgagctaaa
       61 taaaacagca ttatctcatg atatgaaact cgttcttgcc gatcgttttt atttcaaaac
      121 cagtaatcct tgcgaaacaa attcaatttc tgctcgacca gaatcaatgg atattactga
      181 caaagcctat gccgacaaat taaaaaaatt agaaactcct tttcttgatg atcgctattt
      241 ctttatgacc atcaatcagt gtgacggtaa tggcactccc atgctgacag acataaaagt
      301 atgtacaaca gaattatgtg gtgctgaata tacaaagaaa atgtctactg tatggctcga
      361 tgaaacttta aaaccaactg ctaaacgtct ggcaatttat tttattcaat ctcctttacc
      421 ctatgacgag gagaaaaaac aatggaaagt caccggatgg tatatctcat caactgataa
      481 tcagtcttca actaaacaga ttgcatttga aggatataca gacacagata acctctccga
      541 ttctaaattt atttcttcat ttaaatcctg gtttgaaagt ggaaagataa aaaacattgc
      601 taactataat ccaaaaggag aaagagatgg aaaatacgaa agttattaca atagcaatgg
      661 taacgtctat gaatctctaa gtttttctaa tggtcttatc aacggtgagt ttattacata
      721 ttatgaaaat ggggccattg aaagtaaacg tcattttaat aacgacaaaa ttaaagatgg
      781 tgaatgccct catttttatg aagatgggaa aatcaaacag caacatagct atctgaatag
      841 taaactcgat ggtcctgcat atgaatatta tcctgatggc aaactcatgc aggaaaattt
      901 ctatcagcaa agtgtgttga ttggaaacga taccagttac taccaatccg gaaaaataag
      961 ctctatacac aatagaaatt cccgtggtca gtatgatgga gctaacgaac gttatagtga
     1021 agagggtaag ttactgtcta aatccgtcta taaagacgga aaacaaattt ctgttcaaac
     1081 ctggtatgaa aatgggcaga aagaagagga gaaacatttt gatgagcaag gccaattaaa
     1141 tggtctggtt aagcaatggt ataaaaatgg taatcttgca aaatcacaaa actataaaca
     1201 tgacatactc gatggtaaca gtgaagagtg gtacgaaaat ggcatccctg aaagtttgta
     1261 cccttataag aatggaaaaa ccgacggcat tgccaaatca tggaataagt atggtaaatt
     1321 aacctattca atcgaataca aaaatggcgt agagaatggg gcatatcgta actggagcaa
     1381 aaacacaggt aaactaacca aagaaaccct gtacgtcaat ggtatcagac agggagtgga
     1441 aaaagagttt aacgatcgca caggtaaact attaacctcc acgcaatatg ttaataataa
     1501 aagacatggt acggaggaaa cttatgatca aaacggaata aaatatatta catgctatca
     1561 aaatgatcag aaattatcgt ctttagacaa tccaacacaa ataaaagata atgcaacaac
     1621 aggcgattcc agtgctcaat ttgcattagg gaaatacgaa ttcatctgtg caaatataga
     1681 tgaagggatt aaatggttaa ccaaatctgc agagcaaaaa aacactgatg cgatatactt
     1741 tttagctaca gcctacaaag gcaatggtat acctgcaaac aatgaaaaat acctagcata
     1801 tctgcaacaa gctgcaacac tgggcagtag taatgcccag gcggaaattg gctatttata
     1861 tctcatcgga aaagaactac cacagaatct tcctgatgct ggtgtctggt tcaaaaaagc
     1921 agctgcacaa ggtaattttg ccgcacattt ctatctggga aggatgtatc agaatggtga
     1981 cggcgttgag agaaatatgg aaaaagccag gtttcatcta tctaatgcgg cagaaggtgg
     2041 aatagaacct gcgcttaaag cattaaatga acttgaacat caaacaaaat aaagcgctca
     2101 gggaccacgt aaggttcatc aaacagaaaa ggatatagtt tgcgatgaaa gcgaaatcca
     2161 ctttgttatt aatgatttta tctgtttttt attctcatca ggttttatcc agcgaaccca
     2221 ctcagccgga tgtttattct gtcgtcgaga aaaaactaaa ctctgcttta cctctggaag
     2281 aaaataccac cttcagatcg caggctgaat ggtttaattt gcaatatgaa ttattagtat
     2341 ctggctaccc tgaacgtgct tacaatttac tgagcaaact tgagacagaa tcgaaaaacg
     2401 ctaaatatta tctggattta actgcctcca gagtcgctga agaagaagca ccagagacag
     2461 ccttaacttt tttatcaaaa attgggttaa ataaaccggc tactcttaca tcctttgaga
     2521 gctatatcaa tagctggata aaaagcaaac aacccgaaaa ggctatgcag cttctcagcc
     2581 tcgatgagaa tgccctgaat tattatttac cgactgtgtt agtcgcatat cagatcgttc
     2641 ctgataaggc aataagtatt tataattctg tatatggaga taatgttgtt ataccctatc
     2701 agcaagtcac catgttactt acagttgcgg agggttatca aacaaaaggc gatataaaaa
     2761 acgccattca ttatgccgat aaggcgctga atatgtttga taaagctatt gctgtagata
     2821 gcagtcgtga atcccattat agtgatgagt atttgaaact catgaatatc tacgccgcca
     2881 atggcaacaa agaaaaagcg gtggttttat ctcaacgtct acacaaagcc attaccgaat
     2941 ctcaatcgaa ttacttaagt actctggaag gaattttatt gttttataga aataatgata
     3001 tgcagcaaga ctatcaaaac agcctttcaa attacatagt atttatcgat aaaatttttg
     3061 ccttttcccc atcaacaaga agtgaattaa gcctcattaa tttattaaat tcattaaatg
     3121 aggtggaatt gatgagaaaa cgtctggcgt ttctgatgac agcaccagaa tacgcttgtt
     3181 ataatagtca gtattgttat gaagacaaag tcagcgcatt aaaaatcctg tatcgtcatc
     3241 atgacaatga gctaatggac aaatatttca cgactcttct taaagagatc agtaatcaac
     3301 cattagaaga ctgggatata ataataagta atactggtga aaaatttagt gaagtgggtc
     3361 taaccaaata tgctcaacat ctcgcatctt tcgctgaaga attttatata aaagagcaaa
     3421 aatcacattc tgaaagtgaa atgagaggac tattttcatc acttgccgaa ttatatagtt
     3481 ttggtaatga tactgccagc gcaaaccgag tgttccatca gcatgtccct gccttcaata
     3541 cagatcatat gattcaatat tttataaatg ccaaagaatg gaacaatgcc agggaattat
     3601 tgataaaaga ggaaatgttg ggtatgcata atctgatatt gttggagaat atttgtatgc
     3661 aaaaaaatgc agaatgcatg gcacacatta cctttacgct taataaatta acaacacaac
     3721 cagcaataac aaaaatagat agcgtgggta atgagcaact ttatcagatt ggcaaaatct
     3781 accataagtt ggaaattaaa cctgagccag agcaacaggt tttaattcag tcgctttatg
     3841 accgggcatc aggaagcgcc tctgcaactc aataaatgca tggtcaaaag ggccaccacg
     3901 gtggccctga caatcagtag ctatatccag aaagttaatc gcggaagttt ttgaactgga
     3961 acggctgacc gagatcgcca ccgcgcacca tcgccatgac agcctgcaaa tcatcacgag
     4021 atttgccagt cacgcggatc tcatcgccct gaatttgcgc ctgcactttc agtttgctgt
     4081 ctttgatcat cttgacgatt tttttctgag tggcgctctc aatgccctgt ttcagtttcg
     4141 cttccacaaa ccaggtttta ccgctatgaa cgatattttc cggtacatcc agtgaactgc
     4201 cttcaatgcc gcgcttcagc agcttggcac gcagaatatc cagcaactga ttgacctgga
     4261 agtcggactc gctcaacact ttgatggttt tgctggcgtc gttcagctca aatgaggctt
     4321 caacgttacg gaagtcaaaa cgggactcca cttcgcgact cgcgttatcg accgcgttac
     4381 gtgcttcctg aagatcaact tcagagacaa tatcgaaaga tggcatcttt tcctctccct
     4441 tcatttttga tgcgaagcat aatacctgca aagttaaata accaacagtc ctgcaccagg
     4501 acgtatccct taattgtgca tagtcgcaac agcatgcaca attaagggat agccttattg
     4561 aacacccgga gtggttgcgg gtgaggagga acaatgaaaa ttaccgtatt gggatgcggt
     4621 gccttagggc aattatggct tacagcactt tgtaaacagg gtcatgaagt tcagggctgg
     4681 ctgcgtgtac cgcaacctta ttgtagtgtg aatctggttg agacagatgg ttcgatattt
     4741 aacgaatcgc tgaccgccaa cgatcccgat tttctcgcca ccagcgatct gctcctggtg
     4801 acgctgaaag catggcaggt ttccgatgcc gtcaaaagcc tcgcatccac actgcctgta
     4861 actacgccaa tactgttaat tcacaacggc atgggcacca tcgaagaatt gcaaaacatt
     4921 cagcagccat tactgatggg caccaccacc catgccgccc gccgcgacgg caatgtcatt
     4981 attcatgtgg caaacggaat cacgcatatc ggtccggcac ggcaacagga tggcgattac
     5041 agttatctgg cggatatttt gcaaaccgtg ttgcctgacg ttgcctggca taacaatatt
     5101 cgcgccgagc tgtggcgcaa gctggccgtc aactgcgtga ttaatccact gactgccatc
     5161 tggaattgcc cgaacggtga attacgtcat catccgcaag aaattatgca aatatgcgaa
     5221 gaagtcgcgg cagtgatcga acgcgaaggg catcatactt cagcggaaga tttgcgtgat
     5281 tacgtgatgc aggtgattga tgccacagca gaaaatatct cgtcgatgtt gcaggatatc
     5341 cgcgcgctgc gccacaccga aatcgactat atcaatggtt ttctcttacg ccgcgcccgc
     5401 gcgcatggga ttgccgtacc ggaaaacacc cgcctgtttg aaatggtaaa aagaaaggag
     5461 agtgaatatg agcgcatcgg cactggtttg cctcgcccct ggtagtgaag agactgaagc
     5521 cgtcaccacg atcaacctct tggttcgcgg cggtatcaaa gtcaccactg ccagcgtcga
     5581 cagcgatggc aacctggcaa ttacctgctc gcgcggcgtg aagctgctgg cggatgcgcc
     5641 gctggtcgaa gtggctgatg gcgaatatga cgtgatcgtg ctgcctggtg gcattaaagg
     5701 cacggagtgt tttcgcgata gcactctgct ggttgaaacc gttaaacagt tccaccgttc
     5761 cgggcgtatc gtcgcggcta tttgcgccgc gccagccacc gtgctggtgc cgcacgatat
     5821 ctttccgatt ggcaatatga ccggcttccc gacgctgaaa gacaaaattc ccgctgaaca
     5881 atggcaggac aagcgcgtcg tctgggatgc acgggtaaaa ttgctgacca gccaggggcc
     5941 gggtacagct atcgactttg gtctgaaaat tatcgacctg ctggttgggc gcgagaaagc
     6001 ccatgaagtg gcatcacaac tggtgatggc ggcagggatt tataattatt acgagtagtg
     6061 tcggatgcgg caaacgtcgc atctgaccag atgcgacgta aaaactacaa attacgggcg
     6121 atataccttc acattgttaa agccttgctc gcgcagatag agcgcctgca gacggctcat
     6181 caccccgcgc tcacaccaca gcagccaggt tttgttctgg tcgagatcgc caaatttggt
     6241 gctcagttta tagaacggca gagaaaccac atcaatccct tcgactttca gtggcttatc
     6301 ttcctgttca tcgacagaac ggatatcgag aatcacgtcg ttcgggccga agccgttgac
     6361 ggtttccact tccaccactt cctgctcggt ctgctgggcg atttcgcgga tatcaacgtt
     6421 attcgcttcc tcaaccactt tatcgagaat actgaagtcg aacttctctt cttccgcttc
     6481 aatcttcgat ttaaccgctt tcaccgtcgg gcttttggag atcacgccac aatattccgg
     6541 catcgtgcga gcaaagtctt cggtgccaat ctggcgagcc aggttgatga tgtgctcttt
     6601 gtcgtaagaa atcagcggac gcaggatcag cgtgtcagag acgttatcaa tcaggcgcag
     6661 gttggtcagc gtctgactgg acacctggcc gagcgcttcg ccggtgacca gcgcctgtac
     6721 gccgtaacgt tcagccactt tagatgcggc acgcaccatc atacgtttga ggataacgcc
     6781 catctgaccg tcgtcgattt tctcgagaat ttccccgaca accggttcga aattaatggc
     6841 gacaaaacgc acgcggtggg agctgccaaa gcggttccac agataatgcg ccacctgacg
     6901 cacgccaatt tcatgcgctg cgccgccgag gttaaagaag cagtaatgca cgcggcagcc
     6961 gcgacgcatc aacatataac tggaaacacc ggagtcgaaa ccaccggaaa tgagcgacag
     7021 cacatcttcc tgggtgccaa tcgggaaacc accaatacct tcgtagcgac ctttaatcag
     7081 caggaggcga tcgtcttcca cttccagatg aacagttaca tccgggttag tcagcttcac
     7141 gcgcgcggat tcaatatgct gatttaaacc gccgccgacg taacgttcca catcaatcga
     7201 gctaaaatca tgtttgccac ggcgcttcac gcgtacgcag aaggttttgc cttccagctg
     7261 atcgcgatac tgaaccaacg ctttctcaaa aatatcgtgc atgtcggtaa acggcacgtc
     7321 ttcgacttcg agaatatggt ggatacccgg aatacgggtc agggcgtcgc gaatagtcag
     7381 acgctggttt tcatctttcg cgcgaacttc gatgttatcc cagtggcgga cgacagcgag
     7441 cgtctcatca tagtgcttta aaacgttacg aatgttcccg gtaaggattt ttataaagcg
     7501 caagcgcaca gattggcttt tgatggtgat ttccgggaac aatttaatga taaacttcat
     7561 ggcggcaatg gttcgttggc aagccttaag cgacttgtat agggaaaaat acagcagccc
     7621 acacctgcgg ctgcatccag gcgcggaagt ataccactaa catcgctttg ctgcgcacat
     7681 caccttacca ttgcgcgtta tttgctattt gccctgagtc cgttaccatg acggggcgaa
     7741 aaatattgag agtcagacat tcattatgcc gaagaaaaat gaggcgcccg ccagctttga
     7801 aaaggcgctg agcgagctgg aacagattgt aacccgtctg gaaagtggcg acctgccgct
     7861 ggaagaggcg ctgaacgagt tcgaacgcgg cgtgcagctg gcacgtcagg ggcaggccaa
     7921 attacaacaa gccgaacagc gcgtacaaat tctgctgtct gacaatgaag acgcctctct
     7981 aacccctttt acaccggaca atgagtaatg gactttccgc agcaactcga agcctgcgtt
     8041 aagcaggcca accaggcgct gagccgtttt atcgccccac tgccctttca gaacactccc
     8101 gtggtcgaaa ccatgcagta tggcgcatta ttaggtggta agcgcctgcg acctttcctg
     8161 gtttatgcca ccggtcatat gtttggcgtt agcacaaaca cgctggacgc acccgctgct
     8221 gccgtagagt gtatccacgc ttactcatta attcatgatg atttaccggc gatggatgat
     8281 gacgatctgc gtcgcggttt gccgacctgc catgtgaagt ttggcgaagc aaacgcgatt
     8341 ctcgctggcg acgctttaca aacgctggcg ttctcgattc taagcgatgc caatatgccg
     8401 gaagtgtcgg atcgcgacag aatttcgatg atttctgaac tggcgagcgc cagcggtatt
     8461 gccggaatgt gcggtggtca ggcattagat ttagacgcgg aaggcaaaca cgtacctctg
     8521 gacgcgcttg agcgtattca tcgtcataaa accggcgcat tgattcgcgc cgccgttcgc
     8581 cttggtgcat taagcgccgg agataaaggg cgtcgtgctc tgccagtact cgacaagtac
     8641 gcagagagca tcggccttgc cttccaggtt caggatgaca tcctggatgt ggtaggagat
     8701 actgcaacgt tgggaaaacg ccagggtgcc gaccagcaac ttggtaaaag tacctaccct
     8761 gcacttctgg gtcttgagca agcccggaag aaagcccggg atctgatcga cgatgcccgt
     8821 caggcgctga aacaactggc tgaacagtca ctcgatacct cggcactgga agcgctagcg
     8881 gactacatca tccagcgtaa taaataaaca ataagtatta ataggcccct gatgagtttt
     8941 gatattgcca aatacccgac cctggcactg gtcgactcca cccaggagtt acgactgttg
     9001 ccgaaagaga gtttaccgaa actctgcgac gaactgcgcc gctatttact cgacagcgtg
     9061 agccgttcca gcgggcactt cgcctccggg ctgggcacgg tcgaactgac cgtggcgctg
     9121 cactatgtct acaacacccc atttgaccaa ttgatttggg atgtgggaca tcaggcttat
     9181 ccgcataaaa ttttgaccgg acgccgcgac aaaatcggca ccatccgtca gaaaggcggt
     9241 ctgcacccgt tcccgtggcg cggcgaaagc gaatatgacg tattaagcgt cgggcattca
     9301 tcaacctcca tcagtgccgg aattggtatt gcggttgctg ccgagaaaga aggcaaaaat
     9361 cgccgcaccg tctgtgtgat tggcgacggc gcgattaccg ctggcatggc gtttgaagcg
     9421 atgaatcacg cgggcgatat tcgtcctgat atgctggtgg tcctcaacga caatgaaatg
     9481 tcgatttccg aaaatgttgg cgcgcttaac aaccatctgg ctcagctgct ttccggtaag
     9541 ctttactctt cactgcgcga aggcgggaaa aaagttttct ctggcgtgcc gcccattaaa
     9601 gagctgctca aacgtaccga agaacatatt aaaggcatgg tggtgcctgg cacgttgttt
     9661 gaagagctgg gatttaacta catcggcccg gtggacggtc acgatgtgct ggggcttatc
     9721 accacgctaa agaacatgcg cgacctgaaa ggcccgcagt tcctgcatat catgaccaaa
     9781 aaaggtcgtg gttatgaacc ggcagaaaaa gacccgatca ctttccacgc cgtgcctaaa
     9841 tttgatccct ccagcggttg tttgccgaaa agtagcggcg gtttgccgag ctattcaaaa
     9901 atctttggcg actggttgtg cgaaacggca gcgaaagaca acaagctgat ggcgattact
     9961 ccggcgatgc gtgaaggttc cggcatggtc gagttttcac gtaaattccc ggatcgttac
    10021 ttcgacgtgg ctattgccga gcaacacgca gtgacctttg ccgccggtct ggcgattggt
    10081 ggatacaaac ccattgtcgc gatctactcc accttcctgc aacgcgccta tgatcaggtg
    10141 ctgcatgacg tggcaattca aaagctaccg gtcctgttcg ccattgaccg cgcgggcatt
    10201 gttggtgctg acggtcaaac tcaccagggc gcttttgatc tctcttacct gcgctgcata
    10261 ccagaaatgg tcattatgac cccgagcgat gaaaacgaat gtcgccagat gctctatacc
    10321 ggctatcact ataacgatgg cccgtccgcg gtgcgctacc cgcgcggcaa cgcggttggc
    10381 gtggaactga cgccgctgga aaaactacca attggcaaag gcattgtgaa gcgtcgtggc
    10441 gagaaactgg tgatccttaa ctttggtacg ctgatgccag aaacggccaa agtcgccgaa
    10501 tcgctgaacg ctacgctggt cgatatgcgt tttgtgaaac cacttgatga agcgttaatt
    10561 ctggaaatgg ccgccagcca tgaagcgctg gtcaccgtag aagaaaacgc cattatgggc
    10621 ggcgcaggta gcggagtgaa cgaagtgctg atggcccatc gtaaaccagt acccgtgctg
    10681 aacattggcc tgccggactt ctttattccg caaggaactc aggaagaaat gcgcgccgaa
    10741 ctcggcctcg atgccgccgg tatggaagcc aaaatcaagg cctggctggc ataatcccta
    10801 ctccactcct gctatgctta agaaattatt catggcagga gtggcagcat gcaatacaac
    10861 cccttaggaa aaaccgacct tcgcgtttcc cgactttgcc tcggctgtat gacctttggc
    10921 gagccagatc gcggtaatca cgcatggaca ctgccggaag agagcagccg tcccataatt
    10981 aaacgcgcgc tggaaggtgg cataaatttc tttgataccg ccaatagcta ttccgatggt
    11041 agcagcgaag agatcgttgg tcgcgcactg cgggatttcg cccgtcgtga agacgtagtc
    11101 gttgccacca aagtgttcca tcgcgttggt gatttaccgg aaggattatc ccgtgcacaa
    11161 attttgcgct ctatcaacga cagcctgcga cgtctcggca tggattatgt cgatatcctg
    11221 caaattcatc gctgggatta caacacgccg atcgaagaga cgctggaagc cctgaacgac
    11281 gtggtaaaag ccgggaaagc gcgttatatc ggcgcgtcat ccatgcacgc ttcgcagttt
    11341 gctcaggcgc tggaactaca aaaacagcac tgctgggcgc agtttgtcag tatgcaggat
    11401 cactataatc tgatttatcg cgaagaagag cgcgagatgc tgcctctgtg ttatcaggag
    11461 ggcgtggcgg tgattccgtg gagcccgctg gcgcgggggc gactgacgcg tccatgggga
    11521 gaaactaccg cacgactggt gtctgatgag gtggggaaaa atctctataa agaaagcgat
    11581 gaaaatgacg cgcagatcgc agagcggtta acgggcgtca gtgaagaact cggtgcaaca
    11641 cgagcacaag ttgcgctggc ctggttgtta agtaaaccgg gcattgccgc accgattatc
    11701 ggaacttcgc gggaagaaca gcttgatgag ctgctgaacg cggtggatat cactttgaag
    11761 ccataacaga ttgccgagct ggaaacgccg tataaaccgc atgcggtcgt aggatttaaa
    11821 taagagtcca ggcctgatga gacgtgacaa gcgtcacatc aggcatcggt gcacaactac
    11881 gacagaatac ccagcggcca gtgatgaccg ataaagtaca ggatgcctgc ggaaatcacc
    11941 ccggcgacaa tatcgtcgat catgatcccc atgccgccat gtacattgcg atcaaaccag
    12001 cggatcggcc acggcttcca catatccaga atacggaaaa tcacaaaccc ggcggcaacc
    12061 cactgccagt cattggtcgg cagcgccatg agcgtgatcc acataccaat aaattcgtcc
    12121 cagacaatgc tgccgtgatc gtgcacgccc atatctttcg ccgtttgatg acaaagatag
    12181 acgccgatac agatccccaa catcaccacc agcgagtaga gctgccaggg taaaaaggtc
    12241 atcagatacc agaaagggat cgccgccagc gatcccatcg agccaggaac gattgggctt
    12301 aatccacttc cgaatccgac agcaagcaga tgccacggat tactcatctt caggcgactt
    12361 ttcgcgacat ctttatggcg tgacaaaatg gtcatatcct ttccagtcaa acgtgacagg
    12421 ttcgccgtca cgaataaaac aaagcccttc gatatcggcg gtcatttgcc cgatacaggt
    12481 aaacggtacg ccaaggtgtc cgagagcaac atccagcgcg ccacggttca gttccggcac
    12541 cgtgaaacac aactcgtaat cttcaccgcc agagagcgcc cagcgcagcg cctgttccgg
    12601 ttcaacatgg cgagaaagcg catcagaaaa cggcagcaat gccaggtcaa tacgtgcgcc
    12661 gcagtcgctg gctttcacga tatgcccgag atcggaaatc agaccgtcag agagatcgat
    12721 agctgaattt gccagatcgc gcagtgcctg cccctgtaaa atacgcggcg atggacggag
    12781 atgacgtttg atcaagtagt cagcatcttt agcatcggca acctgcaaac ggttttgcaa
    12841 aatcgccagc ccggcggcgc tatcgcccgg tgtaccggtc acatagatcc agtcacccgg
    12901 tttcgcccca gagcgcgtta aggctcgtcc catcggaaca aagccgtgga tacccaacgt
    12961 cattgataat ggcccacgcg tggtatcgcc gccaatgagt tgcatatcgt aataattgag
    13021 aagatcaaac aaactgtcgc tgaaggactc aagccacgct tcgtctacgt ccggtaaggt
    13081 taatgccagc gtcagccagg ccggatcggc ccccatcgct gccagatcgc ttaggttcac
    13141 cgccagtgct ttataagcca gatcagcagg atcgatatca gggaggaaat ggttgcccgc
    13201 caccagcgta tcagtgctga tcgccagggt ctgtttctcg gggatattga gaagagcgca
    13261 atcgtcgccg atgcccagtt cgacatcaag acgagaactt cttacacggt caaaataacg
    13321 ggcaatcagg gagaactcgc cacatgccat acgttatgcc tcagcaggta aaaaagaaaa
    13381 ggccggcgat catggaacag tcttccgtga atctaccggc ctggatatta ctttttgtta
    13441 gggcgaatca caggtgctgc tttatcgagt acgccgttga cgaacttatg gctgtcttct
    13501 gcgccgaacg atttcgccag ttcgatcgct tcgttaatgg ccactttgta tggcacatca
    13561 ctacgtttag acagttcgta cagcgcaatg cgcagtactg ctttttctac ctggcccagt
    13621 tcttccagca ggcgggacag gtagggcttc atcagtccgt cgaggtatgc ggtgttagtc
    13681 gccaccccgg ccagcagctc acggaagtac aggacgtcaa cgtcttttac atcctgttca
    13741 gccaggaact ggtattcaac atcagcgatg tcgttctggg acaactgcca ggagtagagc
    13801 gcctggacgg cacactcacg agcgcggcga cgagcagcag gtttcacgga tttcccctta
    13861 ctaatttcag gccttgatgg ctttcaatac attaatcatt tcaagcgcgg tcagtgcagc
    13921 ttctgcacct ttgttgccag ctttggtgcc agcacgttcg atcgcttgtt caatgctttc
    13981 agtggtcaga accccaaaag caaccggaat ttcgctgtcc tgggcaacat gcgccaggcc
    14041 gttgcttgca ccaccagcga catattcaaa gtgggcagtg ccaccacgaa taaccgtacc
    14101 cagcgcaatc accgcgtcgt atttaccggt tttagccagt gcacccgccg ccagcggcag
    14161 ctcataggca ccaggcaccc aaacaacggt aatgttttca tcttttacct gaccgatacg
    14221 tttcagtgcg tcaattgcac cttccagcag gctgtcattg ataaagttgt tgaaacgcgc
    14281 gatggtgatg gcgacgcgag cgtccggggt agcaacgtta gcttcaataa tgttcatttt
    14341 cttccttcgg gttcgagtat ggccccgcag gggggcggat tttagcataa tatttcgtgc
    14401 gctgcttccc tttcgagccg ggagatcatg cacccactaa atgcaggcaa acatccgggc
    14461 ctacatgacg tatctctttg aatttaaatt ggggggcgtc ggctaatttc tcaagccctg
    14521 gcagcgagca taatccacgg gcgtcgctgc ctaatagttt aggtgcgata tagacaatca
    14581 gctcatcgac taaacccgcc tgtagcaata cgccagcgag cgttggcccc gcttccaccc
    14641 agatgctgtt aatctgctgt ttacccagtt gcatcatcag tacaaccaga tccagatgac
    14701 ctttatgctc tggaatcagc aaggtacgca ccgtttccgg ccactcacga gaatcttcct
    14761 gcgtacgcgc gaaccaggtt tcgccgggct gctgcacaat gcgatgttcc ggcgtcacgc
    14821 gattttggct atcaatcaca atacgtactg gctggcggag attttgttgc gggtagagcg
    14881 cctgagtttg ttcatccagt tcagaccaac gcactgttaa ggctggatca tccgccagca
    14941 ccgtggcgct gctggttaaa atggcatgac tttgtgcgcg ctgtcgttgt acgtcgcgcc
    15001 gcgcctgagg cgaagtgatc cactggcttt cgccgctcgc catcgccgtg cggccatcaa
    15061 gcgatgcgcc aagttttaac tgaatataag gaaagccggt gcgcatccgc ttgagaaagc
    15121 ctttattcaa ttgctcggct tcactcatca tcagcccgtg gctgacgtca atgccagcct
    15181 gttgcagacg gtaaagtcca cgcccagcga cctgcgggtt aggatcttgc atcgctgcga
    15241 ccacgcgcgc tacgccagcg gcgatgagtg cgtcacagca cggtggcgta cggccatgat
    15301 ggctacaggg ttcgagtgtc acatacgccg ttgcgccttt ggctttttca cccgccatac
    15361 gcaacgcgtg tacttcggca tgtggttcac ccgctcgttg gtggtaacct tcaccgacaa
    15421 tttcgccatc tttgacaatg acgcacccga cattcgggtt ggggtgggtg gtaaaacgtc
    15481 ctcgttgcgc cagctttagc gcccgcgcca tgtaatactc gtcctgcacg gcttagtcct
    15541 ccaggcgcgc gatctcttcg ccaaattctt tgatatcttc gaaactgcgg tagacagagg
    15601 caaaacggat ataggcgact ttatcgagct ttttcaattg ctccatcacc agattgccaa
    15661 tcatcttgct cggtacttcg cgctcaccgg tggcgcgcag ctgcgattta atatgattga
    15721 ttgccatttc aacgtaatcg gaactcaccg gacgtttttc cagcgcccgc agcattccgc
    15781 tacgcaattt ctcttcatta aacggttcac gcacgtcgtt gctttttaca acacgcggca
    15841 taaccagctc cgccacttca aaggtggtga aacgttcatt acacaccaga cactgccgac
    15901 ggcggcgtac ggatgaaccc tcgcccacga gacgagagtc aattacctta gtgtccacgg
    15961 cgaaacagaa tgggcaatgc atacggcgtc ctgaccaggt ggttaacgga aatctatttt
    16021 accctgaact gacgatacaa caaaggcgca acgcttttga gataaaggat cgatgctttc
    16081 gctgctttta ccctctacca ttaggctaat tgcgacgaga aagggacgac ctatgaatac
    16141 taacgttttt cgactgctcc tgctgggaag cctgttcagc cttagcgcct gtgtgcagca
    16201 aagtgaagtg cgacagatga aacacagcgt cagcacgctg aaccaggaga tgacgcaact
    16261 caatcaagaa acagtcaaaa tcactcagca aaacaggctg aatgcaaaat ccagcagtgg
    16321 ggtttacctt ctgcctggag cgaaaacacc ggcaagactg gaaagccaga tcggtacttt
    16381 acgtatgtcg ctggtgaata ttacgcctga tacagatggc accaccctta cactacgtat
    16441 tcagggtgag tcgaatgatc ccttgcccgc attcagcgga actgttgaat atgggcaaat
    16501 tcagggaaca atagacaact ttcaggaaat caatgtgcag aatcaattga ttaatgcccc
    16561 tgccagcgtc ctcgccccca gcgatgttga tattccgtta caattaaagg ggatttctgt
    16621 ggatcagtta ggctttgtac gtatccacga cattcaacct gttatgcagt aaacgtattt
    16681 cgggacgatt ttgtgcgtcc cgcaacatct tccccgtcat tttgttactc tgcttacatc
    16741 acctggattg atagtaaaag tttgcaacaa gggcgaaagt cagtacaatc cccgcccgaa
    16801 tgtgtgtaaa cgtgaacgca atcgattacg taaatgatag aactgtgaaa cgaaacatat
    16861 ttttgtgagc aatgattttt ataataggct cctctgtata cgaaatattt agaaacgcaa
    16921 tttgcgcctt tttcactccc gcaagggatt ttcaaacagt ggcatacata tgaaaaaaac
    16981 attactggca gccggtgcgg tactggcgct ctcttcgtct tttactgtca acgcagctga
    17041 aaacgacaaa ccgcagtatc tttccgactg gtggcaccag agcgttaacg ttgtcggaag
    17101 ctatcacacc cgtttcggac cgcagatccg caacgatacc taccttgagt acgaagcatt
    17161 cgctaaaaaa gactggttcg acttctatgg ttatgcggat gcgccggtat tcttcggcgg
    17221 taactccgat gctaaaggta tctggaacca cggttctccg ctgtttatgg aaatcgaacc
    17281 acgtttctcc atcgacaagc tgaccaatac tgaccttagc ttcggtccgt tcaaagagtg
    17341 gtacttcgcg aacaactaca tttacgacat gggtcgtaat aaagatggtc gccagagcac
    17401 ctggtacatg ggtctgggta ccgatatcga cactggcctg ccgatgagcc tgtccatgaa
    17461 cgtctatgcg aaataccagt ggcagaacta tggcgcagcg aacgaaaacg agtgggacgg
    17521 ttaccgtttc aaaattaaat actttgtgcc gattaccgat ctgtggggcg gtcagctgag
    17581 ctacatcggc ttcaccaact tcgactgggg ttccgattta ggggatgaca gcggtaacgc
    17641 aatcaacggt attaagaccc gtactaataa ctctatcgct tccagccata ttctggctct
    17701 gaactacgat cactggcact actctgtcgt agctcgttac tggcacgacg gtggtcagtg
    17761 gaacgacgat gcagaactga acttcggcaa cggcaacttc aacgttcgct ctaccggctg
    17821 gggtggttac ctggtagtag gttacaactt ctgattatga aaatgccggg atttattccc
    17881 ggcatttctg attgttaaca acgtgtgctt tgttcatgcc ggatgcggcg taaacgcttt
    17941 gtccggccca caaaagcacg taaattcaat atattgctga aataacgtag gcctgataag
    18001 cgtagcgcat caggcaattt tacgttttaa tcacttcttc ttgttcatca tcgctttcag
    18061 gtcagcgaat gggttgtact tcgcttcacc gacatctttc tgcgcatctt cccctgcgat
    18121 aacggtcgta ccatactgat ccgcttcggt atatttcgaa tgctcatgat cgtggcaata
    18181 gagacacaac aattcccagt tactgccatc ttccgggtta ttggtatggt cgtgatcaat
    18241 gtggtgaacg gtaagttcac gcaggttaga ataaacaaac tcgcgggaac agcgaccgca
    18301 gacccacgga tagattttta atgctttttc gcgatagccg ctttctaacc gtgcatagtt
    18361 ttttgggatg atagccatga aaagttacct ggatgaaatg tagggttacg cgcagtttcc
    18421 caagatgcgg ggattttcgc aatctccata catcaaaaaa aatcccgatc ttctgaccgg
    18481 gattttcaac atcaacggga acttgattaa ggcagaattg acggctgatc cgccccttct
    18541 ttttccactt tctgctgcaa catgtgttcg cgcttcatac ccagtttcag agccaacgca
    18601 gatgctacgt agatggaaga tgcagtgccg atggaaacac cgataagcat ggtcagcgag
    18661 aagccttcca gtaccggacc accgaagaga tacagcatca ggataaccat caaggtagta
    18721 ccggatgtga tcaaggtacg gtgcagcgtc tgggtcaagg acacgttaaa gatttcgtaa
    18781 ggcgtaccgc gacggatctt gcggaagttt tcacgaattc ggtccgatac cacgatactg
    18841 tcgttaagcg agtaaccgat aaccgacatc aacgatgcca caatggtcag gtcaatctcg
    18901 atatggaata acgacaaaat acccagcgta atgataacgt cgtgcgtcag cgcaataacc
    18961 acccctgccg ccagtcgcca ctcaaagcgg aaacctacgt acacgaggat agacagcagc
    19021 gctgccatca acgccatcgc accggtttgc gcaaggtcag cccccacgct cggaccgacg
    19081 aactcaatac gcttcactgc tgcattctga ttggtggatt cgttaatcac cttcagaact
    19141 tggctgccca gcacctgacc gccggtttcg ccttcagcag gcggcatacg gaccatgatg
    19201 tcgtggctgc taccaaagtt ttgcagcatt ggctcttcaa aaccggcttt ttgcaatgca
    19261 tcacgcatta cgtcaatttc agccggtttt tcgagcgtaa tttcgataac cgtaccaccg
    19321 gtgaaatcca gcccccagtt aaagccgcgc acgcccataa taacgatagc agcgattaac
    19381 agcagaccag agatgccgaa agcccagtag tcccagcgca taaagtcata gactttacgg
    19441 ccgtggttta gttgttcaac agtatattcc tgtgccacat cgcactcctc agattgacag
    19501 cttcttgacg cgcttgccgc catatagcag gtttacgatg gcacgcgtac cgacaatcgc
    19561 ggtaaacatc gacgtcgcca caccgatacc ggtagtaatc gcgaaccctt taattgcccc
    19621 ggtacccact gcgtacagga tgatgacttt aatcagcgtg gtgatgttcg catcgaagat
    19681 agaactgaat gcgccacgat aaccttcatc aattgcctgt tgaacagtac gtccgttgct
    19741 caactcttct ttaatacgtt cgttgatcag tacgttcgca tcgaccgcta ccgcaagggt
    19801 taagacgata cccgcgatac ctggcatact cagtgttgcg cctggcaaca gcgacataat
    19861 accgacgatc aagatcaagt tggcaatcag agcactggtc gcaatcagac caaacttctt
    19921 atagaagatg atcatgaaca gaatagaaac cagcagaccg gcaaggcaag cctccagccc
    19981 ctgctcaatg ttctgcatac ccagggtcgg gccaattgta cgttcttcaa caatctgaat
    20041 cggcgcgatc aacgcaccgg cacgcagcag cagtgacagc tgacgggctt cgttcgggtt
    20101 gttgatgccg gtgatacgga agctgttacc cagacgagac tggatgttgg cgatgttaat
    20161 cacctcttcc tgtttcacca gaaccgcacg accatttgca tctttcttac cgctgtcttt
    20221 gtactccaca aacagggttg ccatcggttt gccgatgttg tccttggtga agttagacat
    20281 aatgttgcca ccagcgctat cgagcgagat gttaacctgc ggctggttgt attcgtcctg
    20341 gctggaagtg gagtcggtga tatggtcacc agtcagaatt acgcgtttgt acagcacaac
    20401 cggctgacct tcgcgggtct gtttcacttc agagtcgccc ggtacgcgac cggatgccgc
    20461 agcggcctga tcaacattgg tgtttaccag acggaattcc agcgttgccg tcgcgcccag
    20521 aatctctttc gcacgcgcag tgtcctgaat accgggcagt tcaacaacga tacggtcagc
    20581 accctgacgc tgaaccaccg gctccgccac gccaagttgg tttacacggt tacgcaggat
    20641 attaatgttc tgctgcaccg catattcacg cgcttcactc agacgagcat cgctcattac
    20701 cgcacgcagc tggttgctgc cctggctgct aatcaccagg tccggatggc gcttgctcag
    20761 atacgcaatg gcttcatcac gagctttagc atcgcggaaa gtgatgctca ggccgtagtt
    20821 gttttcttta cgaacagtgg tatacgggat gcccttttcg cgcaggtcac tgcgtaggct
    20881 atcgatattt tgttcctgga gtttgccaag cgcggtgtcc atatccactt ccatcaggaa
    20941 gtgaacgccg ccacgcaggt caaggccgag cttcatcggc tcagcatgaa tagctgccag
    21001 ccagcgcggc gttgccgggg caaggttaag cgccacgacg tatttgtcac ccataacgcc
    21061 cattaatgct tcacgagcgc gcaactgggt gtcagtggag tcgaagcgcg caagaatagc
    21121 gccctcttcc agtgccacag acttagcagt tattttttct tcttgtaacg ttttctggac
    21181 ctggatcagc gtttgctcac tggcggcgac tccgcgcgca ccagtgatct gaacagccgg
    21241 atcctcacca aacaggttgg gaagcgcata cagcagaccg atgacaatca ccacgatcag
    21301 catgacgtac ttccacaaag gataacggtt taacacggca attcccttag ggaaaaattt
    21361 taattacagc gccttcatgg tgcctttcgg caggacggca gctacgaagt cacgtttaat
    21421 aactacttca gtggtgtcat tcagcgcgat agcaatgtag ccgttttccg ctactttggt
    21481 tacgcgacca accaggccac cgttcgtcag aacttcatca cctttggcaa tggagtccat
    21541 cagctttttg tgttctttgg tgcgcttctg ctgtggacgc aggatcatga aatagaaaat
    21601 cagaccgaat accaccagca tcaaaatcaa agacatcggg ctaccttgcg ccggtgcacc
    21661 cgttgccgct accgcatcag aaataaaaaa gctcattaaa tttccctcat tattaatatt
    21721 aatcaacgtt caaaggtggt acttctcgcc cctgacgctg gtaaaaatca gttacgaagc
    21781 tctctaattt accctcttca atagccttgc gtaaacccgc catcaaacgc tggtagtaac
    21841 gaaggttatg aatggtgttg agtcgcgcgc ctaatatttc gttgcaacgg tcgagatgat
    21901 gcaagtaagc gcgtgaataa ttgcgacagg tgtagcaatc acactcagga tcgagtgggc
    21961 cagtatcgct cttatatttc gcattgcgga ttttcaccac gccatcggtc acgaacaaat
    22021 gaccatttcg ggcgttgcgg gttggcatta cgcagtcaaa catatcgata ccgcgacgta
    22081 cgccttcaac caggtcttcc ggtttaccaa cgcccatcag gtaacgcggt ttgtctgccg
    22141 gaatttgcgg acacacatgc tccagaatac ggtgcatatc tgctttcggc tcacccacag
    22201 ccagaccgcc gacagcgtag ccatcaaagc cgatatctac cagaccttta acagatatat
    22261 cacgtaaatc ttcgtaaata ctgccctgaa ttataccaaa taacgcattt ttgttaccaa
    22321 gactgtcaaa acgctcacgg ctacgcttcg cccaacgcag agacatctcc atagagcgtt
    22381 ttgcgtaatc ccagtcagca ggatacggcg tacactcatc aaagatcatg acgatatccg
    22441 aaccaagatc gtactgaatc tccatcgact tttccgggtc gaggaaaatc gggtcgccgt
    22501 tgatcgggtt acggaagtga acgccctgtt cggtgatttt acgaatatca ccaaggctga
    22561 agacctggaa gccgccggag tcggtaagaa tcggtccttt ccactgcata aaatcgtgca
    22621 gatcgccgtg cagtttcatg atttcctggc ccgggcgcag ccacaggtgg aaggtgttgc
    22681 cgaggataat ttgcgcgcca gtggcttcaa cttcttccgg cgtcatccct tttacggtgc
    22741 cgtaggtgcc aacaggcata aaacaaggcg tttccactac gccacgatca aagaccaggc
    22801 ggccacggcg tgcgcgaccg tcggtggtgt ccagttcaaa tttcattttt tctccactac
    22861 gtcagaaaaa cagtccaacg tttaaaccag cgccgcggaa ttactccccg acgcgctcat
    22921 taattgcctg cggattgtac gtgataaaca tcgcatcacc gtaactaaaa aagcgatatt
    22981 tctcttctac cgccgcttta taggcattca tggtgtgttg ataaccggca aaggccgaaa
    23041 ccagcataat cagcgtcgac tctggcaagt ggaagttcgt caccagcgca tcgaccactt
    23101 tgtactggaa gcccggatag ataaagattt gggtatcgtc aaagaacggt tcgataagat
    23161 cgttttttgc cgcctgtgcc gcgctttcca gcgaacgtac tgaagtggtg ccaaccgcaa
    23221 tcacccggtt accgcgcgct ttcgccgcca gtaccgcgtc taccacatcc tgcggtactt
    23281 cagcgtattc cgagtgcatg atgtgatctt caatggtgtc gacgcgcacc ggctggaagg
    23341 tgcccgcacc aacgtgcaac gtcacaaacg ccatctccac gcctttggcg cgtaattttt
    23401 ccagcaaagg ctcgtcaaaa tgcagaccag cggtcggggc tgcaaccgcg cccggttttt
    23461 cgctataaac ggtttgataa agttcgcggt cagcgtcttc gtccggacga tcgatatacg
    23521 gcggcagcgg catatggccg atgctgttga ggatatccag taccgagcgt tcatcattaa
    23581 attcgacttc aaacagtgcg ccgtggcgcg cggtcattgt tgcgttgata ctttcgtcat
    23641 cgcccagcaa cagttctgcg ccaggttttg gcgctttcga ggcgcgaata tgcgcaagaa
    23701 tgcgtttgtc gtcgagcatc cgctcaacca gcacttcaat cttgccgccg ctggctttac
    23761 gcccaaacag acgcgccggg atcacgcggg tattattaaa aaccagaaga tcgccggggt
    23821 tgagcttatc gagtaaatcg gtgaaagtac cgtgcgtcag cgcgcccgtc ggcccgtcca
    23881 gcgacagtaa acgacagcta ctgcgttcag gcatgggata gtgggcaatc agggattcgg
    23941 gcaattcaaa ggagaaatcg gtaacgcgca tgacactgac tcagactaaa ataagagtcg
    24001 ggtagtctag tgccggggcg ctctccctgc aacctttacc cccctttgga tagaatataa
    24061 tcgatgaatt ttttagctca cctgcattta gcccatctcg cggaaagctc gctttctggc
    24121 aatttactgg ctgatttcgt acgcggaaat cctgaagaaa gttttccgcc cgacgtcgtg
    24181 gctggcattc atatgcatcg acgtattgac tgacaatctg ccggaagtcc gcgaagcaca
    24241 ggagtggttt cgtagtgaaa cgcgccgcgt tgcgcctatt acgctggatg tcatgtggga
    24301 tcactttctt tcccgccact ggtcgcagct gtcgccggac tttccgctac aggaatttgt
    24361 ctgttatgcc cgcgagcaag tgatgacgat tttgccggac tcaccgccac gttttatcaa
    24421 tctgaacaat tacttgtggt cagagcagtg gctggtgcgc tatcgcgata tggatttcat
    24481 ccagaacgtg ttaaacggca tggcaagccg ccgcccacgt ctggatgccc tgcgtgactc
    24541 ctggtacgat ttagacgctc attatgacgc cctcgaaacc cgcttctggc agttttatcc
    24601 gcggatgatg gcgcaggcgt cacacaaggc gttataaaac gttagttcat ccataccgta
    24661 gccgaaatgg caggcaaagc cagaatcccg tcagtcagtt gcccatgccc ttctttgcat
    24721 tgccattgca cggcattgag aaacggtgac gcgggtagca ccacttcact ggcctcgcca
    24781 cggttgattg ccaccagtac acgttgctga ttcagcacgc ggacaaatac cactacgtta
    24841 tcttccgcat acagcacctg acagccgcca cgacgcagcg cctgactttt cttacgcagc
    24901 acaatcattc gccggtacag cgcgaataac gccgtatcct gcttttccac ctgccagggg
    24961 aacggtttac ggcaaaacgg atcgttttta ccatccagtc ccacttcatc accgtaataa
    25021 atgcacggta caccaggcca ggtgaacagc cagaccaccg ccagcggcag gcgcgcaata
    25081 tcccgaccga gcagcgtttt aaatcgcgca gtatcgtggc tatcgagctg attaaacata
    25141 cgtaattgtt gttgatgaga aagccctgcg cggtaattat ccatccaggc catacaggtt
    25201 tgggcgtcaa tttgctgcgg atcataagag atgtcggtat tggcaagaaa tccccacaac
    25261 gggaatgtga agccacgata gttcatggca gcatcttcca catcggcctg taaccattgc
    25321 cgtgcatcgc caaaatgttc gccgacaata tacgcttccg gctgggtttc tttcgccgct
    25381 tcggtgatcc cggcaacgtg ctgcatatta ttgcgcgccc cacccgcctc ccccagcata
    25441 tgcaccacat ccagccgcca gccgtccata ttccacggcg ctttcagcca gtggcggaca
    25501 atactgtctt ccccgcgata aatttcattc accagacttt ccgactgata atccagcttc
    25561 gtcaagctgg catagccaag ccagtcgagc gccgtgccat catcactaaa cgagtaccag
    25621 tcgcgccagg gcgattcaga gttgtgacaa gctccccccg tgccaagatt gtgcctgtca
    25681 aaccaggcat gggaatcgcc actgtggtta aacacgccgt ccagcaccag ccgcattccc
    25741 agctgctgcg tattgtgtcg caaacgcagc aacgccccat caccgccaaa ctgcggatcg
    25801 acatggcgat aatcctcggt atcgtattta tgtacgctgg gagctttaaa caccggattg
    25861 agatacagcg ctgtcacgcc aagctttttc agatacggca gtttttcgct tatcccgtcc
    25921 agatcgccgc catagaacgt tgatccgccc gcctgcgccg tgaccggttc atcccagtca
    25981 cgcaagatga tctcttgtcc ggctgcatga tggtaataga catgatcctg ttcagcttca
    26041 cgaggaagac tacgcgcaaa acgatcaggg aagatctgat aaaaaatctg atccgcagcc
    26101 cattgtgggc cgatatcagg tacatcgacg gcaaactgct ccagtcgtgc cggcggcatt
    26161 cggctgaagc cctgcggtgt aaaccaacgc tggcgatcgt gccacagcag tttgaaactg
    26221 taacgccgcc ggggttgtcc gctggagaga tcaatcgccg cacgccatgc ggtgacgcct
    26281 ggctgcggct gactgcgctg cttatgcatt ggtactgaca tttcttcgtt atcgtgttct
    26341 gtacgcagca taatgcgctg cggtgggtct tcgcccgtca gccacagtgt aatgagcagt
    26401 tgatctttgc tttgtttaac aaatgggggc accggcaggt gccatgcatt taacatcata
    26461 attcccctgt gatgaaaatc tgatcacctt gccatagcct aatgcaggat cactcatcat
    26521 ctgaaggata tttgggggag gaggacgggg gcggataact ttcggaatag ccggatcagg
    26581 atttcctcct gaaccggctt acagaacgca agtattattg gttttcagcc agctgacgat
    26641 cgtggcggcg tttaaacatc cagccaatca acagcacaac aatccacgcg aaaccgacat
    26701 acagcgagat acgcgtatcc gggtgataac caatcaaccc gataataaag agcaggaaaa
    26761 tcagcccgcc gatggtcgtt gctaccccac ccggcacttt aaatttcagc gccttaactt
    26821 cttctggcgg caaacggcga cggaaggcga tttgcgacag caggatcata atccacaccc
    26881 ataccgtggc gaaggttgcc agcgaagcga tcaccaggaa gacgttttcc ggcatgatgt
    26941 agttcagata caccgcaaac agcagcgcgg tagtcatcac cagcaccgta acccacggaa
    27001 taccgcgacg cgacgttttg ctgaaaattt tcggtgcgct gccctgctct gccataccgt
    27061 ggagcatacg gcctacgcca aatacgtcac tgttaattgc cgacagcgaa gcggtcagca
    27121 caacaaagtt aagaatgctg gcggcaaagg taatgcccat atgctggaac gtcagtacga
    27181 acgggctacc ggcagtgcca acctgattcc acgggtagat agacataatg acgaacagcg
    27241 taccgacgta gaacaccaga atacgcatcg gcacggagtt aatcgcacgc ggaatcgatt
    27301 tctcaggatc tttcgcttca ccggcggtaa tcccgataat ttcgatccca ccgtaagcaa
    27361 acatcaccat ttgcaacgac attaccatgc caagccagcc gttactgaag aagccgccgt
    27421 tgctccacag gttatgaata ccggtcggtt gcccgccgtt gccaatcccc cagatgatga
    27481 tgccgaaacc ggcgacaatc atgatgatga tggtggcgac tttaaagaac gagaaccaga
    27541 attccagctc accgaagacc ttcacgctca tcaggtttac ggcgcaaatg atcagcacca
    27601 cgctcagtac ccaaatccag tgcggcaccg tcgggaacaa gacacccata tagataccaa
    27661 aagcggtcac atcggcgatg gcgacaataa ggatttcaaa acagtaggtc catccggtaa
    27721 tgtaacctgc cagcgggcca aggttttcct gcgcataacg cgagaaagag ctggcggccg
    27781 ggttatgtac cgacatttcc cccagcgcac gcataatgat atacgccgcg ataccaccga
    27841 taatataggc caacaacacg ctcggaccgg ccattttgat ggcgtctgcc gaaccgtaaa
    27901 acagcccggt gccaattgct gaacccagtg ccataaagcg tatgtggcgg gtacttagcc
    27961 cacgctttag cttattctta ctttccatca attcctaacc catcaacaca ataaaaaacc
    28021 acggggcatc gcagccccgt ggttaaaaca aatgttcagt gatttagtga gcgctggagg
    28081 tcacctgacg acctgccgcg cgatcccaga taatggccag aaccaccatc accactgttg
    28141 gcattaacca cgccagacct tgttcggcca gcggtaaacg ctgcgcccag gacggtaaga
    28201 tatcgctgaa tgcagatgct ttgatcccgt cgagaatacc aaaaagcagg ctgataaaca
    28261 tcggcggagc aatcacgcgg gacgaattat gccaccatga gcgtgtaaaa cttaatacaa
    28321 ccagtgcgat acacggcgga taaatagcgg tcagcaccgg tacggagatc tggatcagct
    28381 ggcttaagcc gaggttagac accaccatcg agaagccgcc gaggataaac accagcgtac
    28441 gataagagag cggtacgtac tgggcaaaga attctgcaca agcacaggtc aggccaaccg
    28501 ccgtgaccag acaggcgatg aagattaacg ccgccagcag gaagctaccg ccgccgccaa
    28561 aggtgtgctg aacgtaagca tgcagaatag cagcgccgtt tgcagactga tcgaccagcg
    28621 acgcgctgtc tgaacccaga cagaacagcg ccaggtacag caaagtcaga ccaacacccg
    28681 ccatcaggcc agcccagacg gtataacggg tcagcagacg cgcttcggta acgccacgag
    28741 aacgcgccgc gttaacaata acgataccaa acaccattgc gcccagcgta tccatggtca
    28801 gatagccgtt aacgaagccg ttagaaaatg cagcgttttg ataagcctca gtcgccgtgc
    28861 tgatagaacc cgccggccag acaatcgcga caacagacag gatgaccagc gcgataattt
    28921 tcagcggcgc aaggaagttg cccacggtat ccagcagctt gcccggatag agcgaaacca
    28981 gaataacgat agcgaaatag accaggctgt agataaacag cggcagcgcg gaatcacccg
    29041 tcagcggcgc aatccccact tcaaaggaaa cggtagctgt acgcggcgta gcgaaaagcg
    29101 gccccaccgc caggtaacaa accgttgcca gcagtacgcc agcgacttta ccgattggcg
    29161 tgctgaggct gtcaacaccg ccgccaactt ttgccagcgc cactaccgtt aataccggca
    29221 ggccaacggc agtaatgagg aagccgaatg ccgcagtcca gacgtgttcg cctgcctgta
    29281 agccgaccat tggagggaaa ataatgttac ctgcgccgac gaacaacgca aatgtcataa
    29341 agcccagagc gatgatatcg cgcgatctta attgatgggt cataaatcat actgcctgtg
    29401 gatgtggtgt tgtaacgtta aatttttgtg ccaatccctg gggatgatac agcggtatat
    29461 ttaaacaatt ttagcgggaa atactcccaa ccaggcgcgg cgaagaatag tttttcgatt
    29521 taccgcgtta atgcagtcag tatgacagca cctgaagcgc aatttaaacg cttataacgt
    29581 tttaaagcaa ggagggatct aaaaaccaga ggtatatagc gataagaaat caccaaccag
    29641 tataaaacac tatttccgtg aaaaagacat ggctaatcat gcgaacaaaa aatcaccaac
    29701 cgttgtaaaa tctaacgccc gtattttgac tgcaaagaaa ataagccagc atcggctggc
    29761 ttatggcaat aaaagatgac aaaggcggat taatcgctgt ttttggcaat taaacgttcc
    29821 gggataacaa aactgaaacg tgttcctttt cctactgtac tctcaatatt caggcgactt
    29881 tcgtgatgat tcaccgcatg tttcacgatc gctaacccta atccgctacc gccggtttgc
    29941 cgggaacgcg ctttatcaac gcgataaaaa cgctcggtca ggcgcggaat atgctccggt
    30001 gcaatgcccg gtccgttatc ttcaacgcta aattcggcac cgtgcggcac tcgctgccag
    30061 cgtacggtga tatgcgtgcc ttccggcgta tgattcacgg cgttatagac caggttcgaa
    30121 atcgcactgc gtagctgatc ttcgttgcca gacaccttga ggccgttatc tatctcaaag
    30181 gtaaatgtct gttttttctg actcagagtc tgagcctcgc gctcaacaac gcgcagcatc
    30241 atcggcacat caaccttttc attgagcaat tgcgtcggcg cggcttcaat tttcgacagc
    30301 gttagcaact gcttcaccag cccttccatc cgctgggtct gctcgcgcat ggtgtgcaac
    30361 gctttttcgc gtaccgcgcc ttccagcggc tgctcatcca tcatctccag gtaaccctgt
    30421 aacacggtca atggcgtacg taactcatgg ctcacgttgg caaaaaagtt acgccgcgcc
    30481 ccttccagtt gatgcatttg cgtgacatca cgcgccacca tcagcaactg tttgtgggta
    30541 taaggcatga cgcgaatttc cagatgccgc ccggtgttga gcaccagatt gagcgggcga
    30601 gaaaaatcac gcgttttcag atattgcgta aactccgggt aacgcagtag gttaaggatg
    30661 ttctgcccgt tatcttccgg ccagcgcaaa ccaagaattt gttgcgccag accgttacac
    30721 cagaaaatac cgccctcttc cgtggtcagc accaccgcgt cgggcagcga ctccgcgccg
    30781 ctacgaaagc gtttaatcag attgcccagt tcacggcggc gttttttatt tcgcagctgc
    30841 atctggtgta agccgtatag tagcggttcc cagctaccac gccccggtgg cggggtcata
    30901 ctgcgatcca cccacagcca ccatgaaagg cgcaataaat tccagaaatg ccagataagc
    30961 agtcctgtta ccgatgccag caaaaaccag ggcaggtaac caaaaaatgc acccaggatg
    31021 aaagccggga ggcagcaaag tagcagctcc agcaccagcc ttttccacga cagccgttcc
    31081 agcacgtaag atactccagt taagaaatca taagccctgc tctgcgtccg atgagcaagg
    31141 cgttaaaagc gggttgaaaa acgatatcct gtaccgcgca cggtctgcac catgcggtca
    31201 tgcccgccgg gctccagtgc tttacgcagg cgacgaatgt ggacatcgac cgtgcggtct
    31261 tccacataca cgttagttcc ccagacgtgg tttaacagct gctcgcggct gtacacgcgc
    31321 tcaggatgcg tcataaaaaa gtgcagcagt ttaaattctg tcggccccat ctccagcggc
    31381 tcttcgcccg ccatcactcg gtgagatgtc gggttgagac ttaatccctg catcttaatc
    31441 acctcttcca ccgccattgg cgaaatacgg cgcattaccg ctttgattcg cgccaccagc
    31501 tccttcggcg aaaatggctt ggtgatatag tcatccgcgc cggtttcaag gccgcgcacg
    31561 cgatcttctt cttcccctct ggcggtcaac atcaccactg gaatatcccg ggtcatcgac
    31621 tcgcgcttga ggtgtttgat gaactggata ccggagccgc caggtaacat ccagtcaagg
    31681 agaattaaat ccggccaggg ttcattcagt tgattcacag cactgtcata atcttccgct
    31741 tcgaccggct gaaagccatt ttgttcgagc acgaagcaga ccatttcgcg aattggagct
    31801 tcatcttcta cgaccagaat acgtctcgcc atgatttgcc ctgttgtaat aaataggttg
    31861 cgatcattaa tgcgacgtca ttatgcgtca gatttatgac agatttatga aaagctcgtc
    31921 gcacatatct tcaggttatt gatttccgtg gcgcagaaaa aagcaaatgg cacatctgtt
    31981 tgggtataat cgcgaccatg ctttttcgcc agggaaccgt tatgcgcatc cttcacacct
    32041 cagactggca tctcggccag aacttctaca gtaaaagccg cgaagctgaa catcaggctt
    32101 ttcttgactg gctgctggag acagcacaaa cccatcaggt ggatgcgatt attgttgctg
    32161 gtgatgtttt cgataccggc tcgccgccca gttacgcccg cacgttatac aaccgttttg
    32221 ttgtcaattt acagcaaact ggctgtcatc tggtggtact ggcaggaaac catgactcgg
    32281 tcgccacgct gaatgaatcg cgcgatatca tggcgttcct caatacaacc gtggtcgcca
    32341 gcgccggaca tgcgccgcaa atcttgcctc gtcgcgacgg gacgccaggc gcagtgctgt
    32401 gccccattcc gtttttacgc ccgcgtgaca ttatttccag ccaggcgggg cttaacggta
    32461 ttgaaaaaca gcagcattta ctggcagcga ttaccgatta ttaccaacaa cactatgccg
    32521 atgcctgcaa actgcgcggc gatcagcctc tgcccatcat cgccacggga catttaacga
    32581 ccgtgggttc cagtaaaagt gacgccgtgc gtgacattta tattggcacg ctggacgcgt
    32641 ttccggcaca aaactttcca ccagccgact acatcgcgct cgggcatatt caccgcgcac
    32701 agattattgg cggcatggaa catgttcgct attgcggttc ccccattcca ctgagttttg
    32761 atgaatgcgg taagagtaaa tatgtccatc tggtgacatt ttcaaacggc aaattagaga
    32821 gcgtggaaaa cctgaacgta ccggtaactc aacccatggc ggtgctgaaa ggcgatctgg
    32881 cgtcgattac cgcacagctg gaacagtggc gcgatgtatc gcaggagcca cctgtctggc
    32941 tggatatcga aatcactact gatgagtatc tgcatgatat tcagcgcaaa atccaggcat
    33001 taaccgaatc attgcctgtc gaagtattgc tggtacgtcg gagtcgtgaa cagcgcgagc
    33061 gtgtgttagc cagccaacag cgtgaaaccc tcagcgaact cagcgtcgaa gaggtgttca
    33121 atcgccgtct ggcactggaa gacctggatg aatcgcagca gcaacgtctg cagcatcttt
    33181 tcaccacgac gttgcgtacc ctcgccggag aacacgaagc atgaaaattc tcagcctgcg
    33241 cctgaaaaac ctgaactcat taaaaggcga atggaagatt gatttcaccc gcgagccgtt
    33301 cgccagcaac gggctgtttg ccattaccgg cccaaccggt gcggggaaaa ccaccctgct
    33361 ggacgccatt tgtctggcgc tgtatcacga aactccgcgt ctctctaacg tttcacaatc
    33421 gcaaaatgat ctcatgaccc gcgataccgc tgaatgtctg gcggaggtgg agtttgaagt
    33481 gaaaggtgaa gcgtatcgcg cgttctggag ccagaatcgg gcgcgtaacc agccggacgg
    33541 taatttgcag gtgcctcgcg tagagctggc gcgctgcgcc gacggcaaaa ttctcgccga
    33601 caaagtgaaa gataagctgg aactgacagc gacgttaacc gggctggatt acgggcgctt
    33661 cacccgttcg atgctgcttt cgcaggggca atttgctgcc ttcctgaatg ccaaacccaa
    33721 agaacgcgcg gaattgctcg aggagttaac cggcactgaa atctacgggc aaatctcggc
    33781 gatggttttt gagcagcaca aatcggcccg cactgagctg gagaagctgc aagcgcaggc
    33841 cagcggcgtc acgttgctca cgccggaaca agtgcaatcg ctgacagcga gtttgcaggt
    33901 acttactgac gaagaaaaac agttaattac cgcgcagcag caagaacaac aatcgctaaa
    33961 ctggttaacg cgtcaggacg aattgcagca agaagccagc cgccgtcaac aggccttgca
    34021 acaggcgtta gccgaagaag aaaaagcgca acctcaactg gcggcgctta gtctggcaca
    34081 accggcacga aatcttcgtc cacactggga acgcatcgca gaacacagcg cggcgctggc
    34141 gcatattcgc cagcagattg aagaagtaaa tactcgctta cagagcacaa tggcgcttcg
    34201 cgcgagcatt cgccaccacg cggcgaagca gtcagcagaa ttacagcagc agcaacaaag
    34261 cctgaatacc tggttacagg aacacgaccg cttccgtcag tggaacaacg aactggcggg
    34321 ttggcgtgcg cagttctccc aacaaaccag cgatcgcgag catctgcggc aatggcagca
    34381 acagttaacc catgctgagc aaaaacttaa tgcgcttgcg gcgatcacgt tgacgttaac
    34441 cgccgatgaa gttgctaccg ccctggcgca acatgccgag caacgcccac tgcgtcagcg
    34501 cctggtcgcg ctgcatgggc agattgttcc ccaacaaaaa cgtctggcgc agttaatggt
    34561 cactatccag aatgtcactc tggaacagac gcaacgtaat gtcgcactta acgaaatgcg
    34621 ccagcgttat aaagaaaaga cgcagcaact tgccgatgtg aaaaccattt gcgagcagga
    34681 agcgcgcatc aaaacgctgg aagcccagcg tgcacagtta caggcgggtc agccttgtcc
    34741 actttgtggt tccaccagcc acccggcggt cgaggcgtat caggcgctgg agcctggtgt
    34801 taatcagtct cgattactgg cgctggaaaa cgaagttaaa aagctcggcg aagaaggtgc
    34861 ggcgctgcgc gggcaactgg atgcattaac gaagcagctt cagcgcgatg aaaacgaagc
    34921 gcaaagcctc cgacaagatg agcaagcact tactcaacaa tggcaagccg tcacggccag
    34981 cctcaatatc accttgcagc cacaggacga tattcaaccg tggctggatg cacaagatga
    35041 gcacgaacgc cagctgcggt tactcagcca acgacatgaa ttacaagggc agattgccgc
    35101 gcataatcag caaattatcc agtatcaaca gcaaattgaa caacgtcagc agcagctctt
    35161 aacggcattg gcgggttatg cactgacatt gccacaggaa gatgaagaag agagctggtt
    35221 ggcgacacgt cagcaagaag cgcagagctg gcagcaacgc cagaacgaat taaccgcgct
    35281 gcaaaaccgt attcagcagc tgacaccgat tctggaaacg ttgccgcaaa gtgatgatct
    35341 cccgcacagc gaagaaactg tggcgctgga taactggcgg caggttcatg aacaatgtct
    35401 cgcattacac agccagcagc agacgttaca gcaacaggat gttctggcgg cgcaaagtct
    35461 gcaaaaagcc caagcgcagt ttgacaccgc gctacaggcc agcgtctttg acgatcagca
    35521 ggcgttcctt gcggcgctaa tggatgaaca aacactaacg cagctggaac agctcaagca
    35581 gaatctggaa aaccagcgcc gtcaggcgca aacgctggtc actcagacag cagaaacgct
    35641 ggcacagcat caacaacacc gacctgacgg gttggctctc actgtgacgg tggagcagat
    35701 tcagcaagag ttagcgcaaa ctcaccaaaa gttgcgtgaa aacaccacga gtcaaggcga
    35761 gattcgccag cagctgaagc aggatgcaga taaccgtcag caacaacaaa ccttaatgca
    35821 gcaaattgct caaatgacgc agcaggttga ggactgggga tatctgaatt cgctaatagg
    35881 ttccaaagag ggcgataaat tccgcaagtt tgcccagggg ctgacgctgg ataatttagt
    35941 ccatctcgct aatcagcaac ttacccggct gcacgtgcgc tatctgttac agcgcaaagc
    36001 cagcgaggcg ctggaagtcg aggttgttga tacctggcag gcagatgcgg tacgcgatac
    36061 ccgtaccctt tccggcggcg aaagtttcct cgttagtctg gcgctggcgc tggcgctttc
    36121 ggatctggtc agccataaaa cacgtattga ctcgctgttc cttgatgaag gttttggcac
    36181 gctggatagc gaaacgctgg ataccgccct tgatgcgctg gatgccctga acgccagtgg
    36241 caaaaccatc ggtgtgatta gccacgtaga agcgatgaaa gagcgtattc cggtgcagat
    36301 caaagtgaaa aagatcaacg gcctgggcta cagcaaactg gaaagtacgt ttgcagtgaa
    36361 ataactattc agcaggataa tgaatacaga ggggtgaatt atctcttggc cttgctggtc
    36421 gttatcctgc aagctatcac tttattggct acggtgattg gtagccgttc tggtggttgt
    36481 gatggtggta tgaaaaaagt cattttatct ttggctctgg gcacgtttgg tttggggatg
    36541 gccgaatttg gcattatgag cgtgctcacg gagctggcgc ataacgtagg aatttcgatt
    36601 cctgctgccg ggcatatgat ctcgtattat gcactggtgg tggtggtcgg tgcgccaatc
    36661 atcgcactct tttccagccg ctactcactc aaacatatct tgttgtttct ggtggcgttg
    36721 tgcgtcattg gcaacgccat gttcacgctc tcttcgtctt acctgatgct cgccattggt
    36781 cggctggtat ccggctttcc gcatggcgca ttttttggcg tcggagcgat cgtgttatca
    36841 aaaattatca aacccggaaa agtcaccgcc gccgtggcgg ggatggtttc cgggatgaca
    36901 gtcgccaatt tgctgggcat tccgctggga acgtatttaa gtcaggaatg ctggcgttac
    36961 acctttttat tgatcgctgt ttttaatatt gcggtgatgg catcggtcta tttttgggtg
    37021 ccagatattc gcgacgaggc gaaaggaaat ctgcgcgaac aatttcactt tttgcgcagc
    37081 ccggccccgt ggttaatttt cgccgccacg atgtttggca acgcaggtgt gtttgcctgg
    37141 ttcagctacg taaagccata catgatgttt atttccggtt tttcggaaac ggcgatgacc
    37201 tttattatga tgttagttgg gctagggatg gtgctgggaa atatgctaag tggcaggatt
    37261 tcaggacgtt attcaccact gcgcattgca gcagtgactg actttatcat tgtactggca
    37321 ctgctgatgc tctttttctg cggcggcatg aaaacaacgt cgcttatttt tgcttttatt
    37381 tgttgcgcgg gattatttgc cctttcagca cctctgcaaa tattgttact gcaaaatgcc
    37441 aaaggaggag agttattagg tgccgcaggt gggcaaatag cgtttaacct cggtagcgcc
    37501 gtcggcgcat attgcggagg tatgatgctg acgctggggc tggcatataa ttacgtggcg
    37561 ctgcctgccg ccctgctttc gtttgctgcg atgtcatcgt tgctgctgta tggtcgctat
    37621 aagcgccagc aagcggcgga tagtccggtg ctggcgaaac cactggggta gattactgtc
    37681 acggtgcgcg ttgcttattg ccggatgcgg cgtgaacgcc ttatccgccc tacgcggttc
    37741 tggcacattt tgtaggcctg ataagacgcg gcaagcgtcg catcaggcat gatgcgccaa
    37801 ttgcctacgt tttttactct tgtggccata accacgcagc gccgcgtacg ccgctggaat
    37861 caccgtgctt cgccttactc accggcgttt cacattcgcc gccgaagaca aattgtttaa
    37921 tcaactgccc aaccgtttga tataaacggt ctacattgct catcccgccc cccaggacaa
    37981 tcacatccgg atcgagaata ttcacgacat gtgccagcga ttttgccagc cgcagctcgt
    38041 agcgacgcaa tgccagttcc gctaccggat cgctttcttc aaccaggcgg ataatttcac
    38101 tgcctttcag cgcatgtccg ctcaaacgac gataatccgt cgcgaatccc gtgcccgaaa
    38161 taaaggtttc aatacaacct tgtttaccgc aataacaagg gacttcctcg cgataacgca
    38221 gttcgtcttc gtccatccac ggtagcggat tgtgtcccca ctcacctgcc gtgccattgc
    38281 cgccgatatg cgtccgccca ttgaatgcca cgcccgcgcc gcatcccgtg ccgataatca
    38341 cggcaaatac cgtctgcgct cccgctgccg cgccatctac tgcttctgaa accgccagac
    38401 agttggcatc atttgccagc cgcacttccc gctgcaacct cgcgcttaag tctttatcga
    38461 atggctgacc gttgagccag gttgaattga cattcttcac cacaccggtg tagggcgaaa
    38521 tcgaacctgg aatgcccata cctaccgttc cgcgctgccc tgtcgcctgc tccgccatat
    38581 caaccaacgt ggcgatcgtt tcaatagtct gccggtaatc atcacgcggc gtgggcagac
    38641 gatggcggta caactgctcc cctgcatcgc ccagtgcaat cacttcagtt ttggtgccgc
    38701 ctaaatcgat acctatacgc actgtactct ccttattttt ttcaatatca atagcgtaga
    38761 gacggacaac cggattggca atgcaaggcc gccgacaatt cgttatcatg cccgctaaat
    38821 ttaacgacaa ggccgtggaa attatcatgc tgtggttcaa aaatttaatg gtttaccgtc
    38881 ttagccgcga gatttcgctg cgtgcagaag agatggaaaa acagctagcc tcgatggcat
    38941 ttaccccatg cggcagccag gacatggcga agatgggctg ggttcctccg atgggatcgc
    39001 acagcgatgc gttaacgcac gttgccaatg gtcaaattgt tatctgcgcg cgcaaagaag
    39061 aaaaaatcct cccgtctccg gtgattaaac aggcgctgga agcgaaaatc gccaaactgg
    39121 aagcggaaca ggcgcgtaag ctgaagaaaa ccgaaaaaga ttcgctgaaa gacgaagtgc
    39181 tgcactctct gctgccgcgt gctttcagcc gttttagcca gacaatgatg tggctcgaca
    39241 cggttaacgg tttgattatg gtggactgcg ccagtgccaa aaaagcggaa gatacgctgg
    39301 cattactgcg taaaagcctg gggtcgttac cggttgtacc attgagcatg gaaaacccga
    39361 ttgaactgac gctgaccgaa tgggttcgct ccggtagtgc ggcacagggc ttccagctgc
    39421 ttgatgaagc cgagctgaaa tcgttgctgg aagatggcgg cgtgatccgc gcgaagaaac
    39481 aagatctgac cagcgaagag atcaccaatc acattgaagc cggaaaagtg gtgactaaac
    39541 tggcgctcga ctggcagcag cgcattcagt ttgtgatgtg cgacgatggt tcgctcaagc
    39601 gtcttaagtt ctgcgacgag ctgcgcgatc aaaacgaaga tatcgaccgt gaagatttcg
    39661 cccagcgttt tgatgccgat ttcatcctga tgactggtga actggcagcg ttaattcaaa
    39721 acctgattga aggattaggt ggcgaagcac aacgttaatt gctgattttc ctttaatgcc
    39781 ggatgcgacg cctgccgcgt cttatccggc gtacgaagcc acaccaggca tataattatt
    39841 cgctacggcg agcaataatt tttagcgcag caatattatg cgttttacgc cgtaactgat
    39901 ctaatcgcct catccctatt cgcctgtcag cagcacgcag aatattcact aacccccatt
    39961 tatcgctggt aaattgtttt gttaacagct ccgcttttgg ggtatcaata tattcacgca
    40021 ataaatcaga aattgaacaa atatccgtca ggtaaggata ccagcaagtt tcaccgtgaa
    40081 aattacgcac tccagcgtta cctgcaataa agtctttcgg gtaatcccag atcacgtttt
    40141 tatccaacgt gatccaataa cgcggtaaat cggtacttcc accattttgg ctgcgcatgg
    40201 gataacgggt acagtgaatt tgcaggttga tcgttggcgt cagtaagtca tagatctccc
    40261 gctgtaactt gctccatgga cgttgtgtca ttgtttttcc tcaagccggt gacgcgcttc
    40321 actgcgaatt ttccgcgttg ccccggtttt cactaaatca cgtaactgcg aaagatcggg
    40381 ttgttcgaca aataagagta atgatggatc ctcttttaac cacgccgttt tcacatcggc
    40441 agccagctgt ggattattaa tagccagcga ccgatcctga ggctcagtca atgtcgttaa
    40501 caacgacgcc ggggtatgtg cgttgcgtaa tgccgtttgg cgcaaatgat cattgcgact
    40561 ttgcagcaat gcacgccacc attcatcatc tgatttatct tttatcgtag ccagttccgc
    40621 aaaaaatacc tggagatctt cactgatatt tatatcaacc ttgatgaatt atgaatatat
    40681 cggaaattga aaaaatcgca gcaaaggaaa tattattatg ggttaaagag aggatatttc
    40741 agaaaaatgc cctcccgttc aaggggaagg cgaggaatta cagatagcgg cacagataag
    40801 aggtaggttc ggcaacttga agatgaaact cactgtgacc gggaacatta aacaccgaac
    40861 cggcttcata cacttgccag tcggtcgcgt caggcagtaa cacattcagc gcgccactga
    40921 ttaccgtcat ctcttccggc tcagcggtgc tgaaggtgta ttcgccttca accataacac
    40981 ccacgctggc gcgaccagtg ctgctgctgg aaaagccgat cgatttcact ttgccggaaa
    41041 agtactcatt actttgaagc ataaactggc cctgtctggt agttgaacat ctacttcact
    41101 ataggggcca gaggcgctgg ctgtcacgca aaattacacc agtaattccg cagccagccg
    41161 tgcaatcaat acgttagaga gcaagacagg aacatcgagc tgtttttgca gtaaatcgcg
    41221 atgacgttgg tgaaatccta aacaatccag catgatgaca tcagcccctt ttgccagtaa
    41281 ttctttccca gcatcaatga ttttttgttc tgaatcatga atggggttac ccaatgaaaa
    41341 taccggcgat ttctgcaaaa tttgccattt ttgcgcctga acgggcagca tctcctcaac
    41401 cggaacgata acccccacct gatgatcttc aacaatagag gaaaccagtg gaggcaatat
    41461 tcgcgacggc tcaagaaaga tcgtattacg cgcagtcata ctactaatgt ttgctgtact
    41521 cattaataaa atgacgtcat aaccctgatt atcgagcact tcaaccacac cttgcagatc
    41581 tcgctccact ttacgacgcg aaacatgggc cagctggttg tcatttaata atgtgagaat
    41641 ggtgtcttcg cctgcttctg gcgcgtactc cgccatcact tcttcacgac ttaacttccc
    41701 cagcaggcta tgatgggaaa tattatcttc gtcaatgtat tccgtcagga gcggcaaaac
    41761 ctcctgcata ggtacaatgc cgatggtcag gatcgccaac gacgcactca ttgttcatcc
    41821 accttacttt tctttcgtcg ttaattaccg ggcaagtgtg aagcaccact gctgacactt
    41881 acttgtgcta caaatgacaa aaagcgtagc agcagacgtg cacggcataa tgtaaagatt
    41941 ccaaatgatt ccagtaatgg aatgttattg tttaatattc taattattag aaaaacataa
    42001 attatgaaaa atgtgacgca gatcccttgt agtggcactc agaatccctt ccgagcagtc
    42061 tgatgcggtc actctttgtc agggtcctca tagcgttttt tcgcatccat cgcttcctga
    42121 gccgtcggat gttcactgat caacgagtct ggtttaggat gatcggctct gagttggtac
    42181 caggttaccg tctgtcctgg ctttcctttt tcaatcgtca ctatatatgc ttcacgagga
    42241 taaggcggtt tcgttggcat cgttcttcct tatttcacgg gatgaacgtt aagtataggc
    42301 gctctaaaat caacaattga tcgtctgtgc cagggcgcta cgaatttcag aaatcacctg
    42361 gctgggttcg tttgttgcgt cgatgataat atgcgcaact tcgcgatata gcgcatcgcg
    42421 ttcttccagc acttcctgaa cttcttcgct cagcggtttt cccgttaagg ttggccgtaa
    42481 atcttcttcc ggtgcagctt gcagtcggtt aaccaggact gatactggcg cacacaaata
    42541 aaccacgatc ccgttatttt gcatgaagtg acgattaaat tccgtcagaa taatgccgcc
    42601 gcctgtagcg ataacggtgg atgccgcagt taccgcttcc agcgccgccg tttctctggc
    42661 gcgaaatccc gcccactctt ccctttcgac gatctccgcg accgtcatat tgagctgtga
    42721 ttgcaaccac tgatcggtat cgacaaaccg acggttaagc gaatcggcaa gggccattcc
    42781 gaccgttgtt ttaccacagc cccgaggccc gatcagaaaa agaggttgtg tcatcgtggg
    42841 ttttccccaa taggtcgcaa tgcggcgaaa gccggtgtca tgagaatagc gatcatacca
    42901 tcaaactagt acaatttcga ttgtaaagaa aaaattccac ttaaagtaaa aatctcaata
    42961 catcccttac tataccaata aatattcaag aatgaagtgt aaataataaa ttacatttag
    43021 ccacgactac gttgcacttc cagccaccac ttctccagct ccgcggcaaa ggcctggcgg
    43081 tcacgttgtg aaaggctatc tggtccgccg gtctggatcc cactggcacg taaggtatcc
    43141 ataaaatcgc gcatcgtcag gcgctcacga atggtcgctg gcgtgtaacg ttcgccgcgc
    43201 gaattaagcg ccgctgcgcc tttctcgatg gcttcagcag ccaaaggtat atctgcggtg
    43261 atcaccaaat cgcccgcttc acactgccgg acaatttcgt tatcggcaac gtcgaaacct
    43321 gccgcgacgc gcagcgtacg aataaatcgc gatggcggca cgcgtaaact ctggtttgct
    43381 accagtacca gcggcatctg catacgttcc gccgcgcgat acaaaatctc tttaattaca
    43441 ttgggacacg cgtcggcatc cacccaaatt gtcataaagt catcctttgt taggtaatcc
    43501 tctattgtgt cgcgcttttg ccttccggca tagttctgtt tatgcttctg ccagcgatta
    43561 tcaaaacaat gaatttcacg gcaggagtga ggcaatggaa aagaaaatcg gttttattgg
    43621 ctgcggcaat atgggaaaag ccattctcgg cggtctgatt gccagcggtc aggtgcttcc
    43681 agggcaaatc tgggtataca ccccctcccc ggataaagtc gccgccctgc atgaccagtt
    43741 cggcatcaac gccgcagaat cggcgcaaga agtggcgcaa atcgccgaca tcatttttgc
    43801 tgccgttaaa cctggcatca tgattaaagt gcttagcgaa atcacctcca gcctgaataa
    43861 agactctctg gtcgtttcta ttgctgcagg tgtcacgctc gaccagcttg cccgcgcgct
    43921 gggccatgac cggaaaatta tccgcgccat gccgaacact cccgcgctgg ttaatgccgg
    43981 gatgacctcc gtaacgccaa acgcgctggt aaccccagaa gataccgctg atgtgctgaa
    44041 tattttccgc tgctttggcg aagcggaagt aattgctgag ccgatgatcc acccggtggt
    44101 cggtgtgagc ggttcttcgc cagcctacgt atttatgttt atcgaagcga tggccgacgc
    44161 cgccgtgctg ggcgggatgc cacgcgccca ggcgtataaa tttgccgctc aggcggtaat
    44221 gggttccgca aaaatggtgc tggaaacggg agaacatccg ggggcactga aagatatggt
    44281 ctgctcaccg ggaggcacca ccattgaagc ggtacgcgta ctggaagaga aaggcttccg
    44341 tgccgcagtg atcgaagcga tgacgaagtg tatggaaaaa tcagaaaaac tcagcaaatc
    44401 ctgatgactt tcgccggacg tcaggccgcc acttcggtgc ggttacgtcc ggctttcttt
    44461 gctttgtaaa gcgccaaatc tgccgatttc aaccactcac gatagtgact catttgtggg
    44521 ttcagcggcg caacccccac actaatccgt aaagttacct gtggcgtatt cggcaagcgt
    44581 aacgtgttta gcccttcacg cacccgtaac atggcggtaa tggcgctctc agctggcgta
    44641 ccggacatga ttactgcaaa ctcatcgccg ccaaaccgac caatcacatc gctaccgcgc
    44701 agggtaattt gtaactgtcg ggtaagcgcc acaatcgctt catcgcccac atcatgaccc
    44761 caggtatcgt tgatgctctt gaaatggtcg atatcgataa tcagtaacgt tgcatcgcga
    44821 ttatgccgcc gacagttatc aaattcattg cgtaacatag tttcccaatg acgtcggtta
    44881 tacacgcccg tcatgccgtc gcgagtactc atgacctgca acctgcgttt atgttccgcc
    44941 agtttggttg ccgtctggta gctgacccag ccaaacatca gaggataaac gacaataatg
    45001 ggaagggaga gccaccattc cagcggagca ctattgaacg acacggtaat gcccgtaagc
    45061 tcgagggtga caaggcagga aaccaccatc aacaccagac ccgcgacaaa cagacggggg
    45121 ccgcctgccc ccatcaaatt cagacacaga atcatcaaca tagcggtgga aggcagcacg
    45181 tttacgccca ttacgcctac ccacattccc gctaatactg catcggtttt taagttgtaa
    45241 atttcccggc taagcggatc gacggccctg ctcgctatct gccaggctaa atgcggccag
    45301 acgaacgccc agccgaccaa caccagccac caccagcccg gcggcgggtg cgaaaccagc
    45361 gttgaagcaa tcggtaagaa catgccagcc aggccaaccg cacggggtag tctgacgcga
    45421 cgggcgaagc gcagcccgga ccgctgatgt tcgttttgag gagttaaagg aggttcctcc
    45481 ccatgcgccg ccgctttttt gaaaaagttt tcatcattca ttatttttgg gaacattcag
    45541 aaacaatttt cccaaattat agagacgggg ctaaaggcag aagtatgaaa tttcgggtga
    45601 gccactttcg tagctcaccc gttagcact
//
LOCUS       DAJTUI010000025        42790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTUI010000025 DAJTUI010000000
VERSION     DAJTUI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.025"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO464_001479"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO464_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6226678.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="OO464_001480"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="OO464_001480"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6226679.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="OO464_001481"
     CDS             complement(3736..4092)
                     /locus_tag="OO464_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6226680.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="OO464_001482"
     CDS             complement(4096..4512)
                     /locus_tag="OO464_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6226681.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO464_001483"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO464_001483"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6226682.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="OO464_001484"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="OO464_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO464_001485"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO464_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6226683.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO464_001486"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO464_001486"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6226684.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO464_001487"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO464_001487"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6226685.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="OO464_001488"
     CDS             10521..11504
                     /locus_tag="OO464_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6226686.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO464_001489"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO464_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6226687.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO464_001490"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO464_001490"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6226688.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="OO464_001491"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="OO464_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="OO464_001492"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="OO464_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6226689.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="OO464_001493"
     CDS             complement(15306..15515)
                     /locus_tag="OO464_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6226690.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO464_001494"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO464_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6226691.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO464_001495"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO464_001495"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6226692.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO464_001496"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO464_001496"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6226693.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="OO464_001497"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="OO464_001497"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6226694.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO464_001498"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO464_001498"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6226695.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO464_001499"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO464_001499"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6226696.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="OO464_001500"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="OO464_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO464_001501"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO464_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR6226697.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO464_001502"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO464_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR6226698.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO464_001503"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO464_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR6226699.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="OO464_001504"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="OO464_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR6226700.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="OO464_001505"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="OO464_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR6226701.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="OO464_001506"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="OO464_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR6226702.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32406..33881
                     /gene="xylE"
                     /locus_tag="OO464_001507"
     CDS             32406..33881
                     /gene="xylE"
                     /locus_tag="OO464_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR6226703.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="OO464_001508"
     CDS             complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="OO464_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR6226704.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34461..34604)
                     /locus_tag="OO464_001509"
     CDS             complement(34461..34604)
                     /locus_tag="OO464_001509"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226705.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34605..34883
                     /locus_tag="OO464_001510"
     CDS             34605..34883
                     /locus_tag="OO464_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226706.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34930..37026)
                     /locus_tag="OO464_001511"
     CDS             complement(34930..37026)
                     /locus_tag="OO464_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6226707.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAISWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37026..37763)
                     /locus_tag="OO464_001512"
     CDS             complement(37026..37763)
                     /locus_tag="OO464_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6226708.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37760..38398)
                     /locus_tag="OO464_001513"
     CDS             complement(37760..38398)
                     /locus_tag="OO464_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6226709.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38512..38754)
                     /locus_tag="OO464_001514"
     CDS             complement(38512..38754)
                     /locus_tag="OO464_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226710.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="OO464_001515"
     CDS             complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="OO464_001515"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6226711.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41391..42740
                     /gene="lysC"
                     /locus_tag="OO464_001516"
     CDS             41391..42740
                     /gene="lysC"
                     /locus_tag="OO464_001516"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6226712.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT        10643 a        11014 c        10503 g        10630 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcactg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa ttatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gataaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaatagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca gggactggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 caataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagcggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgat
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg gtcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctccctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgcctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gccttttttt ctttcgtctg caatctgaat cgttcgccgg
    32161 ttaatattcc catcatagaa cttattattt ttacgttatt tgttttccca cttacgataa
    32221 ttctctttcg tgctctgagt cacggcaata gtattgtttc atcaattttt gataattatc
    32281 acaattaaga tcacagaaaa gacattacgt aaacgcattg taaaaaatga taattgcctt
    32341 aactgcctga caattccaac atcaatgcac tgataaaaga tcagaatggt ctaaggcagg
    32401 tctgaatgaa tacccagtat aattccagtt atatattttc gattacctta gtcgctacat
    32461 taggtggttt attatttggc tacgacaccg ccgttatttc cggtactgtt gagtcactca
    32521 ataccgtctt tgttgctcca caaaacttaa gtgaatccgc tgccaactcc ctgttaggat
    32581 tttgcgtagc cagcgctctg attggttgca tcatcggcgg tgccctcggt ggttattgca
    32641 gtaaccgctt cggccgtcgt gattcactta agatttctgc tgtcctgttt tttatttctg
    32701 gtgtaggttc tgcctggcca gaacttggtt ttacctctat aaacccggac aacacagtac
    32761 ctatttatct ggcaggttat gtcccggaat ttgttattta tcgtattatt ggcggtattg
    32821 gcgttggttt agcctcaatg ctttcaccaa tgtatattgc ggaactggct ccagctcata
    32881 ttcgcggaaa actggtttca tttaaccagt ttgcgattat tttcgggcaa cttttagttt
    32941 actgcgtaaa ctattttatt gcccgttccg gtgatgccag ctggctgaat actgacggca
    33001 ggcgttatat gtttgcctcg gaatgtatcc ctgcactgct gttcttaatg ctgctgtata
    33061 ccgtgccaga aagtcctcgc tggctgatgt cacgcggcaa gcaagaacag acggaaagta
    33121 tcctgcgcaa aattatgggc aacacgcttg caactcaggc agtacaggaa attaaacact
    33181 ccctggatca tggccgcaaa accggtggtc gtctgctgat gtttggcgtg ggcgtgattg
    33241 taatcggcgt tatgctctcc atcttccagc aatttgtcgg catcaatgtg gtgctgtact
    33301 acgcgccgga agtgttcaaa acgctggggg ccagcacgga tatcgcgctg ttgcagacca
    33361 ttattgtcgg agttatcaac ctcaccttta ccgtactggc aattatgact gtggataaat
    33421 ttggtcgtaa gccactgcaa attatcggcg cactcggaat ggcaatcggt atgtttagcc
    33481 tcggtaccgc gttttacact caggcatcgg gtattgtggc gctactgtcg atgctgttct
    33541 atgttgccgc ctttgccatg tcctggggtc cggtatgctg ggtactgctg tcggaaatct
    33601 tcccgaatgc tattcgtggt aaagcgctgg caatcgcggt ggcggcccag tggctggcga
    33661 actacttcgt ctcctggacc ttcccgatga tggacaaaaa ctcctggctg gtggcccatt
    33721 tccacaacgg tttctcctac tggatttacg gttgtatggg cgttctggca gcactgttta
    33781 tgtggaaatt tgtcccggaa accaaaggta aaacccttga ggagctggaa gcgctctggg
    33841 aaccggaaac gaagaaaaca caacaaactg ctacgctgta atcttcctgt ccagcacgcc
    33901 gcgccatttc ggcgtgctgt ctttttactc ccgcttcagc cgtttcgaat tacacagcca
    33961 cagggtgatc accagtaaca ggatcgcagc cgagtagatc aacacatcca gtggcgattt
    34021 atgatcgacg atgatcaagc gcacaatggc ggtgatccca atatagacaa agtaacgtag
    34081 cgggaagtga aaaccggact gaaagtactt cacaatcagc gcgataaatt cgaaatagag
    34141 aaagtaaacc accagtcctt ctaccagctc atatttgctg gtttgttctg gcgcgaacag
    34201 cacatcagcc agatgcaccg tttctttgcc gaggaagaca accaaaatca ggccgaggca
    34261 cagcaggcca agattgagta cggtctgcaa aatggtggag ataaactcca cgcgcggacg
    34321 agagagtgac gtcatagcag tacctccttc acagtggcga gcttcctgta taacgcaaaa
    34381 atgtgacgga gatctatatt ttttgtgtat gttttgttca acctggattt ggagcgcgcc
    34441 tccaccaaag ccggatatac ctacccgtag gccggataag ccgcgcctgg cggcgcatcc
    34501 ggcaagacaa accgcaccaa agccggtaat tcaaaaatca cccattcagg tgattcccag
    34561 ccaccagcaa tgcgcgaaaa atcgacaatg ttcctctgga acaaatgaaa cgtaacctga
    34621 taaaggtggt aaaaatgaag ccttattttg ctgctttgat gttatcagtc tctgttcttc
    34681 ctgcttatgc tggtccgctc ggtactgctg ataaagccga cctaccgcaa agcaatgtct
    34741 ccagcccaat gatggcccag tcgctcaggc aaccagacct gcaaccgata tcgacggaca
    34801 ggaagacaga atgttttcgc ctttacacac cagatcgtaa accgggggtg aactgcgttc
    34861 ctgatggctc aacaggtcat taagaatgag ggtgccagtg agtgcgaaaa ctggcacctt
    34921 tcccatgact tagcggaaat ttacgctacg gtcgctggtc atgtcataca actggaactt
    34981 acgcccaagt tgctgaccac cgtcacgcgt cagcggcgtc cagctaatcg ccgcacggct
    35041 gcgtgtcggg ccagacgaga agagatccaa cggtaccgag acatacacgc ctttggtgaa
    35101 gtccccttcg ccgtactcct ctttcgaaac gttggtgatc gtggcatagc cacccaccac
    35161 cacgccgctg tcaaagcgtt tagcgatctc cagcgtgccg cctttatccc ctgccagata
    35221 ctgcccgacg ctggctttca ccaacacgtc ctgggcgaaa gatggcgtcc agtaggcggt
    35281 cagatgtccg gttttcacgc tgtagtcggt gaatttcatc atatcttttg cactacgcca
    35341 gtcgcgctgt ttaacgtagt tggcatccag accaaacgcc cagttactgt ccagcggacg
    35401 atacaacact tcggcccccg caccgccaaa catggtttcg agataacctc catagacctg
    35461 accgtagaag ccgttgccca gatgctggaa gtagttggct tgcaggttat tcacatagac
    35521 atcgttctgc acatactcgc gcacatgggt acgaacgcgc ggtaagtgcg agtcctgcgg
    35581 tggattggtg tagttaaact tgtcgtagtt gttggcaaga tttgcaaaca ggctgccggt
    35641 ggtcagtaga tggtccgtca gccacaaatc tgccgttccc atcacgccca gctgatacat
    35701 gtaaaagttt tccgggccac cgaccgactg gttcagcacc ggatcgatat ggaaatcaaa
    35761 gcgtgatttg tcgatatacc agccctgctc ggtggacttc ggaaccactg gctcgacgcg
    35821 tttgtgcgcc agcgtcgttt cgtggcccaa cggctctccg gcgagatgat ttttcaggct
    35881 ggcgacatcg gtttccgtcg tcacttgcgg catgttaagg cgattttccg taatgcggat
    35941 cgttttgatc ccatccggca gatcgttcat cacgatccga ttagcacgga tgatcccttc
    36001 gcgcgaatca cgatatttca cctgctcgcc ggtaacatac agcgtatcgc ctttcgcctg
    36061 gatctgcgga tcggcaagtc cggcgttgta tttcagcaac gttaactgat tcgccaccac
    36121 cgaatgctgc aaaatggcat cctgcggctg cggttgatat tgcgggcggg cgttatcgat
    36181 gtaggacggg cgcagatcgt taaagttggt gcgcagcgtg acgccaaaca taaaggtgtt
    36241 gccacgttca tagctaaggt taacgtcggc ccaatcggta acgcgataaa tcgccccgac
    36301 gttaaactta cttttttgct ccagcttccc ggcaaaatcc tgctgataat tattaccttc
    36361 atactccagt ttcagacgca gcggttgcca gggcgtctgg tattccacgc cgccaaacag
    36421 tgaggcagga ccgtggaaca tctggctacc gtcgatagat cccgcctgtt tgtagctgtt
    36481 atcgcgatag caatatttat cactggctga acagagcgga ttttttacat taccgctggt
    36541 gcccagatat ccccagccca ggccgagcgt aaaatcgaac ggcccccagg ctttgctggc
    36601 aacaagatat tccgcatcaa acagccccgt accgccgata tcccgcgcac caaccgccac
    36661 ttgcggcagc cagtaactct cttcccacaa acgcagtttg agatcgaagg ctttatcttt
    36721 atacgtttga tcgccagaga acgcttcgac gctgctgtac tgccgggtgc gcacgtcggt
    36781 gtagcgcagc gttgtttcca gccacgggaa gagttgcact gaagctgagt aataacggta
    36841 ctgatcgtta tcgcgatagt tcagactcaa ctccccttcc cgtgccatac gcgcggtggg
    36901 agtttgtaat aatcctacgc caccgaaatc cgactgcgac ggaccaatgg gcgcaggata
    36961 tgtttcgccg tagcaagctg tgctaatgcc cagcgccagt aagctaagca gatgtctttt
    37021 tttcattatt ggggtatccg ctgcgtcaga gtctgaagaa tgtcggcgtt aagggcatca
    37081 ggcgtctcac tccagacgga atctgcgagg ccaacataaa taatgctgcc gggcatcggc
    37141 tctacgtgac gcttgttcca gtaagccacc ggcgcttttt gcgtgcgtcc gtccgggtaa
    37201 acgacccacg cgtagctgcg atccgcaccg ctgagcaggc tctggtcaga gagatagctc
    37261 gccacgtcgc gacctggagt gaatggctga ttgccaggat ggctgataag cccgaacaac
    37321 gtgaccgtgg acggcggtgg tccgacccac agcgtgtagt tgccctgcaa cggcgggtta
    37381 ccgcgttcgg caacgcggac gatatcggga tcaagattga ttttttgcct gcctgtcact
    37441 ttcaatgcct ggatttgctg gcgtaaggcg ttaatggcgg cggcatcgtc ggtgctggaa
    37501 tctgcgccct gttctgccag ccgtgtcagc aacgcctgct gctgacgcaa tgctgccgcc
    37561 gttgccagct cttcgctaat caccgcacca ggccaccagc tgtttgccag tcgaggttgc
    37621 cctaccagat cgattaaatg ctctgcgccc gttagcgttt tggcctcact gctgccattg
    37681 ctgaacacct tgacggttcc tgccgcgaag accgatgacg ctcccacact caaaagcaac
    37741 gcgacaatag tttgtttaat cattgttttg ccgccttgat gagagtggtt ttcaccggga
    37801 aataatccgc gccgagatac tgttccgact ggcgaatttg cccttcgctg tcgatccagt
    37861 agcggttatg ccagcgcgcc tggtcggtgg agacttcttc gtccagcacg cgaaccaggg
    37921 tttcatcgct gccgaccttg acggtatcgg tgccatccca tttgaagact gagcgtgcgg
    37981 tggcgtagcg tacctgctgg tactcggtcc agcccatcgt gcgcgtccat gttgcaccat
    38041 caacaatttg tgcgggttta atcagcgggt cggcggcaag gttattcact tcaatcaggt
    38101 tgtcgccgcc aagcaaagtc ttcaccagac gaccatgttg tgtgacaaga gtggcttgat
    38161 cctgagtgac ccatttttgt tgtccgtctt cagcgaaggc cagcaccaca aacaattgcg
    38221 gcccgccatt aagctgcatg tactggctgg cgtagggcat attttgaata tcatcgtccg
    38281 tcagctgtac gcctggcgtg ccgaacagac tgtcccacag tgaattgccc agctctttag
    38341 tcgtggctga acaggcctgt aatagcaggc agataagaat gagtgcaggt cgcttcacga
    38401 ctcttctcca gaggggcaga ataaccacac cggagtgtgg ttatggtaaa tcccttcata
    38461 cttcaagcca cagatgcaat gactcaaatt attttgggga tacatacttt attactgggt
    38521 actggtggtc gtggttgtgg tggtcccggt attagaacca tcaccgccac cggttgccgc
    38581 gagcgcgaca cccaccgccg agcttacggt gctgacgctg gtcgcggagg aactccccgc
    38641 cgaaaccgac gttgctgccg actctgccgc ttcgcccacc tgtacaggtg cagcccacgc
    38701 agaagtcgcc gcaagcgcag atatggcaaa aatgccatac agaacttttt tcataacaat
    38761 ttcccttcat tgaatgaatg gagatttacc caaaaacatt tcgggcggga tcgagtatac
    38821 acaactaaag catgcgaatt acggcgcggg gaattagcga tggagtttta ggataattgg
    38881 aatgaatatc aaatataagt ttcaacttat atttaaataa caataaaaat cataagctta
    38941 aaataaaaaa caaaagtaat taatctgcgt attctccgaa ataaacaacg attaaaaatc
    39001 tattttagga ttaacctcac ggtatgattt ccgttaaatc acagacaagg ggatttatct
    39061 gataaaacaa tgccgactta ttgtcggcat tgtttttaag gttatttaac tcctcatact
    39121 atgcattaat gtcgatattg gcatcaggct ccggttgccg gatgcggcgt aaacgcctta
    39181 tccggcctac atatcggcat caggcacgca tgatgattaa ccgcgccacg ctttatagcg
    39241 gttaatcaga ccattggtcg agctatcgtg gctgctgatt tctttatcat ctttcagctc
    39301 tggcagaata cggtttgcca gttgtttacc cagctccacg ccccactggt cgaaggtgaa
    39361 gatgttcagg atcacgccct gagtaaagat tttgtgctca tacagcgcaa tcaacgcacc
    39421 caggctgaac ggagtgattt cgcgcagcag gatggagttg gtcgggcggt taccttcgaa
    39481 cactttgaac ggcaccacgt agtcaagcgt tgccggatct ttaccctgat cgcgatattc
    39541 ctgctcaacc acttcgcggg atttaccaaa cgccagcgct tcggtctggg cgaagaagtt
    39601 agacagcagt ttctggtggt gatcagagag cgggttatgg gtgatagccg gggcgatgaa
    39661 atcgcacggt accattttgg ttccctggtg gatcagctgg tagaacgcgt gctgaccgtt
    39721 agtgcctggt tcaccccaga taatcgggcc agtctggtaa tccacaacgt taccgttacg
    39781 gtcaacatac ttaccgttgg actccatatt gccctgctgg aagtacgccg cgaaacggtg
    39841 catatactgg tcatacggca gaatcgcttc agtttccgca ccaaagaaat tgttgtacca
    39901 gatgccaatc agcgccagca ggacaggcag gtttttctcg gcaggcgtgg tggagaaatg
    39961 cttgtccatt gcgtgtgcgc cagaaagcag ttcaacgaag ttatcaaagc cgatggagag
    40021 aacaatcgac aggccaatcg ctgaccacaa agagtaacgg ccgccgaccc agtcccagaa
    40081 ctcgaacatg ttggcagtat caataccaaa ctcgccaacg gctttggcat tggttgagag
    40141 cgccgcaaaa tgtttcgcaa cgtgtttttc atcacctgcc gctttcagga accagtcacg
    40201 cgcgctatgg gcgttggtca tggtttcctg agtggtgaag gttttagatg ctaccaggaa
    40261 cagcgtggtt tccgggttta cttttttcag cacttccgag atgtgagtcc catcgacgtt
    40321 agaaacaaag tgcatgttca ggtggttttt gtacggacgc agtgcttcgg tcaccatgta
    40381 cgggccgagg tcagaaccgc cgatcccgat gttcactacg tcagtgattg ctttgccggt
    40441 ataacccttc cactcaccgg agataatcgc ttccgagaag gttttcatct tctccagcac
    40501 cgcgttgact tctggcataa catctttgcc atcaaccaga atcggggtat tgctacgatt
    40561 acgcagcgct acgtgcagca cggcgcggtt ttcagtgcgg ttgatcttct cgccagagaa
    40621 catcgactta atcgcgcccg ccagatcgca ctctttcgcc agatcctgta atttcgccag
    40681 cgtctcttca gtgatgcggt ttttggagta atccaccagc atctgatcgt cgaaggttgc
    40741 ggagaactta gaaaaacgat cgccgtcttt agcaaaaaga tcggcgatcg taacgtcttt
    40801 catttcatcg aagtgtttct gtagtgcctg ccaggcagcg gtctgcgttg gattgatgtt
    40861 tttcattagc aatactcttc tgattttgag aattgtgact ttggaagatt gtagcgccag
    40921 tcacagaaaa atgtgatggt tttagtgccg ttagtgtaat gttgagtgta aacccttagc
    40981 gcagtgaagc atttattagc tgaactactg accgccagga gtggatgaaa aatccgcatg
    41041 accccgtcgt tgacaaccgc cccgctcacc ctttatttat aaatgtacta cctgcgctag
    41101 cgcaggccag aagaggcgcg ttgcccaagt aacggtgttg gaggagccag tcctgtgata
    41161 acacctgagg gggtgcatcg ccgaggtgat tgaacggctg gccacgttca tcatcggcta
    41221 caggggctga atcccctggg ttgtcaccag aagcgttcgc agtcgggcgt ttcgcaagtg
    41281 gtggagcact tctgggtgaa aatagtagcg aagtatcgct ctgcgcccac ccgtcttccg
    41341 ctcttccctt gtgccaaggc tgaaaatgga tcccctgaca cgaggtagtt atgtctgaaa
    41401 ttgttgtctc caaatttggc ggtaccagcg tagctgattt tgacgccatg aaccgcagcg
    41461 ctgatattgt gctttctgat gccaacgtgc gtttagtagt cctctcggct tctgctggta
    41521 tcactaatct gctggtcgct ttagcggaag ggctggaacc tggcgagcga ttcgaaaaac
    41581 tcgacgctat tcgcaacatc cagtttgcca ttctggaacg tctgcgttac ccgaacgtta
    41641 tccgtgaaga gattgaacgt ctgctggaga acattactgt tctggcagaa gcggcggcgc
    41701 tggcaacgtc tccggcgctg acagatgagc tggtcagcca cggcgagctg atgtcgaccc
    41761 tgctgtttgt cgaaatcctg cgcgaacgcg atgttcaggc acagtggttt gatgtacgta
    41821 aagtgatgcg taccaacgac cgatttggtc gtgcagagcc agatgtagcc gcgctggcgg
    41881 aactggccgc gctgcagctg ctcccacgcc tcaatgaagg cttagtgatc acccagggat
    41941 ttatcggtag cgaaaataaa ggtcgtacaa cgacgcttgg ccgtggaggc agcgattata
    42001 cggcagcctt gctggcggag gctttacacg catctcgtgt tgatatctgg accgacgtcc
    42061 cgggcatcta caccaccgat ccacgcgtag tttccgcagc aaaacgcatt gatgaaatcg
    42121 cgtttgccga agcggcagag atggcaactt ttggtgcaaa agtactgcat ccggcaacgt
    42181 tgctacccgc agtacgcagc gatatcccgg tctttgtcgg ctccagcaaa gacccacgcg
    42241 caggtggtac gctggtgtgc aataaaactg aaaatccgcc gctgttccgc gcgctggcgc
    42301 ttcgtcgcaa tcagactctg ctcactttgc acagcctgaa tatgctgcat tctcgcggct
    42361 tcctcgcgga agttttcggc atcctcgcgc ggcataatat ttcggtagac ttaatcacca
    42421 cgtcagaagt gagcgtggca ttaacccttg ataccaccgg ttcaacctcc actggcgata
    42481 cgttgctgac gcaatctctg ctgatggagc tttccgcact gtgccgggtg gaggtggaag
    42541 aaggtctggc gctggtcgcg ttgattggca atgacctgtc aaaagcctgc ggcgttggca
    42601 aagaggtatt cggcgtactg gaaccgttca acattcgcat gatttgttac ggcgcatcca
    42661 gccataacct gtgcttcctg gtgcccggcg aagatgccga gcaggtggtg caaaaactgc
    42721 atagtaattt gtttgagtaa atactgtatg gcctggaagc tatatttcgg gccgtattga
    42781 ttttcttgtc
//
LOCUS       DAJTUI010000026        40152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTUI010000026 DAJTUI010000000
VERSION     DAJTUI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.026"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..587)
                     /locus_tag="OO464_001517"
     CDS             complement(<1..587)
                     /locus_tag="OO464_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR6226713.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALI"
     gene            833..1459
                     /gene="gstB"
                     /locus_tag="OO464_001518"
     CDS             833..1459
                     /gene="gstB"
                     /locus_tag="OO464_001518"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR6226714.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1456..2570)
                     /gene="yliI"
                     /locus_tag="OO464_001519"
                     /pseudo
     CDS             complement(1456..2570)
                     /gene="yliI"
                     /locus_tag="OO464_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2681..3064)
                     /gene="bssR"
                     /locus_tag="OO464_001520"
     CDS             complement(2681..3064)
                     /gene="bssR"
                     /locus_tag="OO464_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6226715.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3277..4602
                     /gene="rimO"
                     /locus_tag="OO464_001521"
     CDS             3277..4602
                     /gene="rimO"
                     /locus_tag="OO464_001521"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6226716.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4649..5977)
                     /locus_tag="OO464_001522"
     CDS             complement(4649..5977)
                     /locus_tag="OO464_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6226717.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(5985..8327)
                     /locus_tag="OO464_001523"
                     /pseudo
     CDS             complement(5985..8327)
                     /locus_tag="OO464_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8505..9416)
                     /gene="gsiD"
                     /locus_tag="OO464_001524"
     CDS             complement(8505..9416)
                     /gene="gsiD"
                     /locus_tag="OO464_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6226718.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9419..10339)
                     /gene="gsiC"
                     /locus_tag="OO464_001525"
     CDS             complement(9419..10339)
                     /gene="gsiC"
                     /locus_tag="OO464_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6226719.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10357..11882)
                     /gene="gsiB"
                     /locus_tag="OO464_001526"
                     /pseudo
     CDS             complement(10357..11882)
                     /gene="gsiB"
                     /locus_tag="OO464_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
     gene            complement(11902..13773)
                     /gene="gsiA"
                     /locus_tag="OO464_001527"
     CDS             complement(11902..13773)
                     /gene="gsiA"
                     /locus_tag="OO464_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6226720.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(13760..14725)
                     /gene="iaaA"
                     /locus_tag="OO464_001528"
     CDS             complement(13760..14725)
                     /gene="iaaA"
                     /locus_tag="OO464_001528"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6226721.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            14929..16164
                     /gene="moeA"
                     /locus_tag="OO464_001529"
     CDS             14929..16164
                     /gene="moeA"
                     /locus_tag="OO464_001529"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6226722.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16164..16913
                     /gene="moeB"
                     /locus_tag="OO464_001530"
     CDS             16164..16913
                     /gene="moeB"
                     /locus_tag="OO464_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6226723.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17092..17754)
                     /gene="fsa"
                     /locus_tag="OO464_001531"
     CDS             complement(17092..17754)
                     /gene="fsa"
                     /locus_tag="OO464_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6226724.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            17885..18784
                     /locus_tag="OO464_001532"
     CDS             17885..18784
                     /locus_tag="OO464_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6226725.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            18790..21222
                     /locus_tag="OO464_001533"
     CDS             18790..21222
                     /locus_tag="OO464_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6226726.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21368..22183
                     /gene="ybiV"
                     /locus_tag="OO464_001534"
     CDS             21368..22183
                     /gene="ybiV"
                     /locus_tag="OO464_001534"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6226727.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22335..23600
                     /locus_tag="OO464_001535"
     CDS             22335..23600
                     /locus_tag="OO464_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6226728.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(23841..25433)
                     /locus_tag="OO464_001536"
     CDS             complement(23841..25433)
                     /locus_tag="OO464_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6226729.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            25652..26572
                     /gene="ldtB"
                     /locus_tag="OO464_001537"
     CDS             25652..26572
                     /gene="ldtB"
                     /locus_tag="OO464_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6226730.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(26630..27748)
                     /locus_tag="OO464_001538"
     CDS             complement(26630..27748)
                     /locus_tag="OO464_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6226731.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(27745..28212)
                     /gene="mntR"
                     /locus_tag="OO464_001539"
     CDS             complement(27745..28212)
                     /gene="mntR"
                     /locus_tag="OO464_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6226732.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28398..28526
                     /gene="mntS"
                     /locus_tag="OO464_001540"
     CDS             28398..28526
                     /gene="mntS"
                     /locus_tag="OO464_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6226733.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            28798..30381
                     /gene="opgE"
                     /locus_tag="OO464_001541"
     CDS             28798..30381
                     /gene="opgE"
                     /locus_tag="OO464_001541"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6226734.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30429..30944)
                     /gene="ompX"
                     /locus_tag="OO464_001542"
     CDS             complement(30429..30944)
                     /gene="ompX"
                     /locus_tag="OO464_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6226735.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(30997..31062)
                     /locus_tag="OO464_001543"
     CDS             complement(30997..31062)
                     /locus_tag="OO464_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226736.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31297..32184
                     /gene="rhtA"
                     /locus_tag="OO464_001544"
     CDS             31297..32184
                     /gene="rhtA"
                     /locus_tag="OO464_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6226737.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32483..32986
                     /gene="dps"
                     /locus_tag="OO464_001545"
                     /gene_synonym="pexB"
     CDS             32483..32986
                     /gene="dps"
                     /locus_tag="OO464_001545"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6226738.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33390..34136
                     /gene="glnH"
                     /locus_tag="OO464_001546"
     CDS             33390..34136
                     /gene="glnH"
                     /locus_tag="OO464_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR6226739.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34275..34934
                     /gene="glnP"
                     /locus_tag="OO464_001547"
     CDS             34275..34934
                     /gene="glnP"
                     /locus_tag="OO464_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6226740.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            34931..35653
                     /gene="glnQ"
                     /locus_tag="OO464_001548"
     CDS             34931..35653
                     /gene="glnQ"
                     /locus_tag="OO464_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR6226741.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            35914..38138
                     /gene="ybiO"
                     /locus_tag="OO464_001549"
                     /pseudo
     CDS             35914..38138
                     /gene="ybiO"
                     /locus_tag="OO464_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38135..39061)
                     /gene="rlmF"
                     /locus_tag="OO464_001550"
     CDS             complement(38135..39061)
                     /gene="rlmF"
                     /locus_tag="OO464_001550"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6226742.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39337..39597
                     /locus_tag="OO464_001551"
     CDS             39337..39597
                     /locus_tag="OO464_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6226743.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            39862..>40152
                     /locus_tag="OO464_001552"
     CDS             39862..>40152
                     /locus_tag="OO464_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226744.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKN"
BASE COUNT        10004 a        10605 c         9778 g         9765 t
ORIGIN      
        1 tggatcaatg ctttacccag caatgccata tcgcgcgcgg tactgaactg ccccggcgca
       61 tccaggccgt gcaccgtctg gaaggtagtg ttggtcagcc ccagtttttt ggcataacca
      121 ttcatcagac caataaagga ctcctggctc ccggcaacgt aatctgccag cgcaatacag
      181 gcgtcattac cggactggat aatcacacct ttgttcaagt ctgccaccga aacctgatcg
      241 cccggtttga ggaacattac cgatgaacca cgcagtgccg gatttcccgt cgcccaggca
      301 tctttaccga ccgtcaccat atcggtgagt ttaatcttat cggccttaag cgcctgccca
      361 accacatagc tggtcatgat tttagtcagg ctcgcaggat ccagtttctc atccgcattg
      421 ccttctgcca gcactttacc gctggcgtaa tccattaaaa tccatgcacg cgcatccacg
      481 ctcggcgctt caacggtttg ttcggccgcg aatgccgttg gggcaaaaag gaataaaaat
      541 gcagaacccg ctgcaagacc acgaaggaga gaggagtatt gcgtcataaa tgccacccga
      601 gtatccattc aaaaaaatac gttatatcgc cgtatgataa gttcggtgat taataacgca
      661 cacacaaact taaagaaacc gtaagttggt aaagttttta aagtttatgc aatatcctgc
      721 cgcttcgctg cttctggtgt catttttttg ccagctgttg cttaaaagtt aactttactt
      781 caccatcgga gtcctgttca gtctgtggct gcctgtcgga aaaggggtga gtatgattac
      841 gctgtggggt cggaataatt caactaacgt aaaaaaagta ttgctgacgc tcgaagaact
      901 ggaactacct tatgagcaaa ttctcgcggg ccgtgagttt gggataaatc acgatgctga
      961 ttttctggcg atgaacccta acgggctggt gccgttgttg cgtgacgacg aaagtgatct
     1021 cattctttgg gaatcaaacg ccattgtccg ctatctggcg gcacagtacg ggcaaaaacg
     1081 cctgtggatc gactcaccgg cacgtcgtgc ggaagcggaa aaatggatgg actgggcaaa
     1141 ccagacgctc agtaatgctc atcgcgggat cctgatggga ttagtcagaa caccaccgga
     1201 agagcgcgat caggccgcca ttgatgccag ttgcaaagag tgcgacgccc tgtttgccct
     1261 gctcgatgcg gaactggcaa aagtaaaatg gttctctggc gacgagtttg gtgtgggcga
     1321 tatcgctatc gcaccgttca tctacaattt gtttaacgtt ggcctgacct ggacaccgcg
     1381 tccaaatctg caacgctggt atcagcaact cactgaacgc cccgcagtgc gcaaagtggt
     1441 gatgattccc gttagctaat tgcgtgggct aactttaagt aattccccac tggactcgtc
     1501 ggtgagaacg tataaataac cgtcgggtcc agtgcgaaca tcacgaattc gctgccctct
     1561 gtccgttaaa atacggccat cttctgtcac tttgtcgccg ttgacgctca tcacaatgac
     1621 atctttatct ttcagctccc aataaataat ttttgctgcc actgggggaa tttatcgctg
     1681 ttatagaagg ccatgccgct cacagcgggc gaatctttcc agtaaaaaac aggttgctcg
     1741 gtcccggcga cgatctcccc tttcgcttcc ggtatcttaa agcctgaata gttgattccc
     1801 caggttgcca gcggccagcc gtagtttttg cctttttgcg ggatattaat ttcatcacca
     1861 ccgcgcgggc catgttcatt cagccacagt gcattactcc acggattcat cgccattcct
     1921 tgcggattac gaatgccata agaccagatc tcggcgcgcg caccggattc ctttataaaa
     1981 ggattatcat ccgggatttc gccctggtcg gtcagacgca ccagtttgcc ctgtaattta
     2041 tccagatcct gcgccgtcgg gcgctgattg ttttcgccca gagcaataaa aagataacct
     2101 ttaccgtcga ataccagccg cccgccaaaa tggttgccgg tagacagttt tggcatctgg
     2161 cggaagacgg tgcggaagtc ggtcactttt gagagatcat cacttaagcg gccataaccc
     2221 acagcagttc cggctttgcc atcatcgcca acttcggaat aacttaacca gatgcggcga
     2281 gactgagcaa aatcaggcgc taaaaccacg tccagcaggc cgccctgccc gtgcgcccaa
     2341 acgtccggaa ctccggaaag cggcgcagat aatccttttc ctgcttgcca gtgacgcaac
     2401 tcgccgccgc gcagagtgat taacataccg tgattatcgg gtaaaaaggc cagtgcccag
     2461 ggatggtcga gtttgtcttg cagtacttcg acatttaccg ttgcaggagc cgcccagaga
     2521 gcggaagaaa gacaaataag gggcacaagg aaaaaggatt gtcgatgcat aacgcctccg
     2581 gattgactct ggcttaaagc gtagtcagtg gaggagataa caaattcatt tttacaaaaa
     2641 cttaaacatg aagggggaga cgctttctcc cccttagttt tcaggccttc tcaagcatgg
     2701 cgtgcttctg caggctctgg atactcagcg ttaagctcat cagacaattt tcaagcttat
     2761 cggcgttgac ggtaataaca gtcgggcaat catggtgccc actcatcaaa catactgcgg
     2821 ctgtcgctaa tgcttcttca gcatgatgaa gagcactcca ctcttcctga tccagatgaa
     2881 gattcaaccg cagcgattta tcgtgcagtt cgcgattcag tttaaaaaag ttatctcgta
     2941 gatgattgct ttcgctgacg gacatgtatc cttttgcctt cctcagttgc acgtatgcag
     3001 cgaggtcaac gcgtgcgtcg atcaaccggt tcagcagatc gattcgctgt ctgtcaacga
     3061 acatacttcg atcctcctct ttccagcctt gttgcaatta aatagtatgg ctattttttg
     3121 agcaaaatgc tgtccaggat caattcttca cgccgaatta acaaaattta catttccttc
     3181 gcctccgcca gcgaatgaga ttgcgataca aattttatcc agaactctgt agaatccctg
     3241 ccctgacctt tcctgtaact gaatattttt taagctatga gcaaagtaac tccccagccg
     3301 aaaatcggct ttgtttccct tggctgtccg aaaaaccttg tcgattcaga gcgtattctc
     3361 accgaactcc gcactgaagg ttatgacgtg gtaccgagct atgacgatgc gaacatggtg
     3421 atcgtcaaca cctgcggctt tattgacagc gcggtacaag aatcactgga agccattggt
     3481 gaagcgttga atgaaaacgg caaggtaatt gtgaccggtt gtctgggggc aaaagaagat
     3541 cagatccgcg aagtccaccc gaaagtgctg gaaatcaccg ggcctcatag ctatgagcag
     3601 gttctggagc acgttcatca ctacgtgcca aaaccgaaac acaacccatt cctgagcctg
     3661 gtgccagaac aaggtgtgaa actgacgccg cgtcattatg cctatctgaa aatttcggaa
     3721 ggctgtaatc accgctgcac cttctgcatt attccgtcta tgcgcggcga cctgatgagc
     3781 cgtccgattg gcgaagtgtt aagtgaagcg aaacgtctgg tagatgcggg cgttaaagag
     3841 attctggtga tctcgcagga tacttccgcc tatggcgttg atgttaaaca tcgtactggc
     3901 ttccacaacg gcgagccggt aaaaaccagc atggtcagcc tgtgcgaaca gttatcgaaa
     3961 ctggggatct ggacacgtct gcactacgtt tacccttatc cgcatgtgga cgacgtcatc
     4021 ccactgatgg cagaaggcaa aatcctgccg tatctggaca ttccgttgca gcacgccagc
     4081 ccgcgcattc tcaaactgat gaagcgtccg ggttctgtag atcgccaact ggcgcgcatc
     4141 aaacagtggc gcgaaatctg cccggaactg accctacgct caacctttat tgtcggcttc
     4201 cctggcgaga cggaagaaga tttccagatg ctactcgact tcctgaaaga agcacgtctg
     4261 gatcgcgttg gctgctttaa atacagcccg gttgaaggtg cagacgccaa tgccctgcct
     4321 gaccaggttc cggaagaagt gaaagaagaa cgctggaacc gtttcatgca gctgcagcag
     4381 cagatttccg ccgagcgcct gcaagagaaa gtgggccgtg aaattctggt gattatcgac
     4441 gaagtggacg aagaaggcgc gattggtcgc agcatggcag atgcaccgga aatcgacggc
     4501 gcggtttatc tcaatggtga aaccaacgtt aagccgggtg atatcctgcg tgtgaaagtc
     4561 gagcacgccg atgagtacga tttgtggggt agccgggttt aattaattca ggccgggcaa
     4621 aatcatttgt ccggcctgcg aataatgatt actgccagct aatctgtgcc ctgccgtttt
     4681 tcttactctt atacagccgc tcatccgcca gcattttcaa gcgttcgacg gtagtacatt
     4741 catttgaatg cgctacaccg atactggtgc ttaaattaat gcttcccacc tggaaatgaa
     4801 cgatttcttt tagtatcgat agcaactctt ccagcgtgcc gccagtataa atcaccgcaa
     4861 attcgtcacc tccgaagcgg tagactttac ctttatctac cgggatctgt gatttaagga
     4921 ttttcaccac tttcatcaac acttcatcgc ccagcaaatg accgaaggtg tcgttaatgt
     4981 ttttgaattt atcgacgtca aacagacaca aataaccgcc tttttccggc agcgctttta
     5041 aatcttcatc aaaggatttt ctgcccattg ctttggtcag gtcgtccagg aacgccgagt
     5101 tatacatact gtagagtttt ttgacatcgg cagccgaaaa gaaaagcccg ccatccagta
     5161 cgcgttcaaa atggttatta atgatggctt ctttctccac aataccacga ttaatcactc
     5221 tgcgtaccag atagaggcaa ctggcaccca atatcactgc caccaatgcg gcaaagaaaa
     5281 taatcaatgt acaaacccgc aaataaacat cttttttata cgcaaatacg tacaatccgg
     5341 tcggcaaata ggcgaagtgc tccatcgctt ccgaaacggt gaagatggcg tttttagcat
     5401 tatattcatc accggtcacc ggtttaccgt tcttaaatag agagaacatc aggttctcgc
     5461 tgtccatcac cagcgatttt gacagtaaag aaaaacgctc cagaccaagc aaaaatatca
     5521 gactacgatt gtgagttacc ggatagaggt aatagacgaa ggttttaccg tcaaaagaga
     5581 taaaccctgt atttttgaca tgtcgatact tcacaacctg cataatatct gcacaataat
     5641 ttttctcgaa ctcctttacc tgaatacatt cgtcactgga aatcagataa ggaccgacat
     5701 cgtacaaata agagggggaa accgagtcgg cgtaagctgt gcgcaaacca accaacagct
     5761 gttcctccgt gggatttagc gtgttgataa gatcaattgc cacgctacgc cgatattcag
     5821 caatatttgc caccgcaaaa gcagtatcaa taacggactg tttcaggcta tacacccgtt
     5881 cctttaccat ttgagtgtag atcccgcagg caactgctga aatcatgata aaaatcagca
     5941 gactgactgt aagtacgaac ttattgattc tggacatacg taaactactc ttttactaat
     6001 tttccacttt tatcccaagc ggagaatggc atgggtttct ggtacagata cccctgagcc
     6061 tgaacacatc ctaatttaat taattcgcgc gcaacgtctt cgctctctac gccttccgca
     6121 atcaccggga tccggttata gcgtgccaga tcgaaaataa tgcgcacaat ttttcccgca
     6181 gtaccgaaaa tatcgccggt aaattgcgag tccaccttta tgctgtctgg attaatttca
     6241 taaaaacgtt tcaggtttga caaaccggtg ccaaagtcat caatcgatat ccgtacccct
     6301 tgtttacgca gttgttcaat attgagataa aaatgttcat taattcgctc gatcacggtt
     6361 tcagtgatct caacacagag gctgttcgcc ttcaggccat tttctttcat gatggtggta
     6421 aaacgctcat gaaagtgcgg ttcgtttaac tgccagggcg aaacattaat atgcagtaag
     6481 aaatcatcct tcagcgacgc acgttgacta aaatgtctga attaacccat tgccgtttta
     6541 ataatctgat aacccagctc attgataaac ccgatatctt cagcaatggt aatgaacttc
     6601 agcggtgaaa tgatcccccg ctccgcagaa acccaacggc acaacgcctc cgcgctcaga
     6661 atggcgcggg tgttaatatc aacgatcggt tgatagacga ggaatagttc gccaatttcc
     6721 attgcatggc gaatattacg cgcaatccag atattctctt ctatttcgtt gagtttcgcg
     6781 tcatcacaga aaataaactc ggagaacata tggtcacgga tactggaaat cgccagtttc
     6841 agtttttctg cgtaactcca ggcgttttcc ccctgcaaag gttcacaaat ggcagactta
     6901 tggatgcgca gattgcggta gccaaagctg ctgtcaaaat cgtttaccat cgctatttta
     6961 cgggtaaatg tctgtacatt ttcccgacac agaagtaaat agagatcggc attgaatgtc
     7021 acgatatcgc agcattgcgc atatttctcg gcaattcgct gacacaattt cctgacgatt
     7081 ttattgcgtt catcaacacc aaacaccgcc tccacggctt ccagaccaca cagtttgatg
     7141 atccccacag ccagatactg attattgttg gactcaataa tcgccatatt tttaataaaa
     7201 ccgctttcct gatcgtaaga gatcagtgag agcaatttat ttgaccgttc aatttcggca
     7261 tctttcattt ccgcgatcga atttttgatg ctgataatct ctttcgcatc gccttcgcga
     7321 attttcagag cctcgatctg atccgttgag tcgggcaaaa acgccagcgt attaaccagc
     7381 tcggtcattt cagaaaattg ccgacggaac acacgagtgg tcatgaaata taccaccagc
     7441 aacacgataa taatgaacgc aaacggtaga taaaaaagtt tatctgatgc gttcaataaa
     7501 ttaccgccag gataataaaa atagatttcg aacaatgcat tatttggcac tgctgatgtc
     7561 tttgcaatat actccgtatc gacagaatca ataatattat aattaccttc gctattataa
     7621 ataatcagtt tttcacgagc gagttgattc ttggcaatga tctcatgacc ttgtttcaaa
     7681 aatagaactt catcattagc gctgatttga tcaagatact cgttcagata ccgccagttt
     7741 aaatcgacat gaattttatc taccgctaac aaatcgtgat ctttgccgtt gatacgtagc
     7801 gtaaaagaga tacatttttt acccggacga tcaaagcaat tgtatgccgg attccagctg
     7861 ttgttagagt catctgaagc caccgaccac ggtcgtttcg atgggttaaa atcggcaaaa
     7921 ataacccgcc ctgactcgtc gacaagcggt ccatttactt gttcctgatg ataaacaacg
     7981 attttatcat tcgccctccg cgaaaagaga taatatttat tatcatcaag taccagtccc
     8041 accgagtcta aaaatggcat catttccatt ttcagaatca cggcgcgttg aatctcctct
     8101 gtatttttta ataaatacag tgagtccttt aatgtcaggg ccgaacgttc aatatcttta
     8161 aagaaatagc caacgttttt ctgaataagt tcaaagcggc tatcggcacg ttttaccgcg
     8221 cgttccgata ccagttgata gttaatcatg aaaaaaagac caataaatga cagtgctgcc
     8281 aataaaaata aaattatcag ccttatcttt tcgtataaac tcagcatgtt aatacattta
     8341 acaaaataac tatctgaacg ctgtgccgct gcaaaaatag tcccttgctg cggcttttta
     8401 accaaattac ccgattctac aaaatcagca gaaagttaca acacttgctt agggcaataa
     8461 tttcctgtga ggtgattacc ctttcaagca atattcaaac gtaactatcc tttaattttc
     8521 ggatccagcg catcgcgtaa accatcgccc aacaaattga acgccagtac ggtcagaaaa
     8581 atagccaggg ccggaaaaac agcgacatgc ggcgcgataa ccatatccgc tcgtgcctca
     8641 ttgagcattg ctccccactc tggtgtcggc ggctgcgcac cgaggccgag aaatgacagg
     8701 ctggcggcag agataatcga ggtgccaatg cgcatggtga aaaacaccac gatagaagag
     8761 acggtcccag gcaggatatg acgcaataaa atggtcatat cgctggcacc aatactgcgt
     8821 gctgactcaa taaaggtttg ctgtttcaac accagcgtgt tgccgcgcac caggcgggca
     8881 aacgcgggga tggaaaaaat ggcgactgcg ataatcacgt tagcaatgcc gcttcccaac
     8941 accgcaacaa cagcgatcgc cagtaaaata cccgggaagg caaacagcac atcgcaaatg
     9001 cgcatgatca gccgatccca ccagccttca taatatccag ccaacaagcc cagcaacgtc
     9061 cctatcgcca caccgataaa cacggcaaac acgcccgccg ccagcgagat ttgcgcacca
     9121 accaggacac ggctgaaaat gtcgcgcccc agtgaatcga cgccaaacca gtgctgcaaa
     9181 gaaggtccgt tattcagatt gtcataatca aaataatttt cggcgtcata gggagcgatc
     9241 cagcgtgcaa aaatggccac cacaatcaat aaaataacga ataatgcggc ggtcatcgcc
     9301 atatgctgac ggcgaaatcg tcgccagaat tcatgccacg gtgtacgtac ctggtcaggt
     9361 ttgaccagtg gcatggcgtt taacaccgcc tgacgtcgcc agttaaatag tcgcatcctt
     9421 acttgtacct gatagccggg ttaatggcgg cgtaaagcac atccaccact aagttgataa
     9481 gaataaattc cagcgagaaa agcagaattt ccgcctgaat caccgggtaa tcgcgcattt
     9541 ctacagagtc aacgagtaag cgtccaagtc cgggccagtt gaaaactttc tccaccacga
     9601 tagaaccacc gagcaaaaag ccaaactgta agcccatcat ggtcactacc gggatcatcg
     9661 cgttgcgtag cccgtgtttg aggacaaccc aggtttcgct cacccctttc gccctcgcgg
     9721 tacgcatata atcttcgctt aaaacgtcga caaatgacgc acgggtaaag cgcgccatca
     9781 cggcggccac tgccgcgccg agcgtcaggg agggtaaaat gtagtgctgc caactgtctg
     9841 ctcccacagt aggcagccag cccagttcaa cggagaatac ctgaattaaa aacatcccca
     9901 gcgcaaatgc cggaaacgag atccccgaca ccgcaatggt catactcaat cgatccggcc
     9961 aacgattacg ccagacggcg gcgataattc ccgccgccat accaaatatc accgcccaga
    10021 ccatactggt tatggtcagc catagcgttg gcataaagcg gctggcaatc tcatcggcaa
    10081 ccggacgacg cgacaccatc gacaggccaa aatccccctg cacagcattg ctgatatagt
    10141 gccagaactg gtgatacagc ggctgatcca accccagctg ctgacgcacc agttctataa
    10201 cctgcgcatc agcttcgggc ccggcaatca atcgcgccgg atcgccgggc agcatgtgga
    10261 caaataaaaa caccagcacc gagacgataa acagcgtcgg aatcaacccc agtaatcgtt
    10321 tgataacgta ataaagcatt ccactccctg cgttgcttat tgcaaatccg cgtcttcaaa
    10381 gctgaagccg gtatctggca tgatccaaaa accggtcagg tttttactgt gtgccgacac
    10441 cagtttttct accaccagcg ggatccacgg cgattcttgc cagatgatat cctgcgccgc
    10501 cttatataag cgggtctttt ccgccggatc attcgttttc agtgcctgag ccaggaagtc
    10561 atccacctgt ttattgctgt aaaacgcggt attaaacagc gtcggtggcc agttttgcga
    10621 ggcaaacagc ggcgatagtg cccagtcggc ttcgccggtt gaagccgacc agccagtgta
    10681 gaacatccgc acgccactct ctttttgccc tttaccttca acttctgccg cccgctgtcc
    10741 ggcatccatc gcggtcacct gggctttaat cccgacctgc gctaactgct gctgggtaaa
    10801 ttgcagcact ttctgcgcgg tgctgtggtt atgtgacgac cacagcgtgg tactgaaacc
    10861 gttgggatat cccgcctctt tcagtaattc gcgcgctttc actggatcgt aaggccacgg
    10921 tttataactt tgcgcgtagg cgatacttgg cggtaccaca ccagtagctg gagttgcata
    10981 gcccgcaaag gcaactttca ccagcgccgg acggttaatg gcgtaattca gcgcctcacg
    11041 gaccttcggg ttatcgaacg gcttttgcgt cacgttcata ctgatataac gctgcataat
    11101 tgacggactg gccatcaact cgatattttt gtttttctcc agcagtgtgg cctgctcgta
    11161 gggaatgggg aaagcaaact gcgcttcacc ggtttgcagc attgccgcgc gggtgttgtt
    11221 atccgccacc ggacgccagg ttatgctgtc cagtttgggc aatcctggct gccagtaacc
    11281 cgcgaatttt ttcaccttca caaaatcggt ctgattccag gtatccagtt catacggtcc
    11341 ggttcccacc ggatgaaaac caatctcctt gccatatttt tccagcgctg tcggtgaaat
    11401 catcgcggtc gccggatggg caagaatatt aataaacgct gagaacggct gtttgagggt
    11461 aatctttacc gttgtcgcgt tttggcaata ttcttataca ggttatagcg tttaagatga
    11521 ttcgccggat cgctggcccg gtccagattc gcttttaccg ccactgcgtt gaaatcggtg
    11581 ccatcctgga atttaattcc ttcccgcaat ttcacggtgt aagtaatgcc gtcatcggaa
    11641 acggtataac tctccgccag cacgtttttc agtttcatct ctttatccag accgaacagc
    11701 ccctggtaaa acgatttcgc tacggcctga gataacgtgt cattcgcgtc atacggatcg
    11761 agcgtggtga aattcgatcc taccgccacc accacatctt tggcagcgaa tgcacaagat
    11821 gccatcaacg ctgtcgcaat gcccagcgcc actaacccac tacggtgtac agctcttgcc
    11881 attttattct ccgcctgaat gttatctacg catgaatgcg tattctgatt gtggttgtgc
    11941 gacgtaatgc cccggcccga cgcattgcaa cgagacggct gccacctctt cgccacgcag
    12001 atgaatattg ctgggaagat cgtccgacag cagtacacgc tgcggtcgtt gtcgggacgg
    12061 ttcagcgacc ggaactgccg ccagtaattt acgcgtataa ggatgctgcg ggttttcgaa
    12121 gaccgcgcac cgtgaaccaa tttcaacaat ttgcccgaga tacatcaccg ccacacgatg
    12181 actaatccgc tctaccacag ccatatcgtg ggagataaac agatacgcaa tgccgaaatc
    12241 acgctggaga tcgagcaaca agttgataat ctgcccgcga atagaaacat ccagcgccga
    12301 aacggcttcg tcggcaatga tcacttttgg attcaatgcc aacgcgcgag caatgcagat
    12361 gcgctggcgc tgaccgccgg aaaactcatg cgggtaacgc caggcatgtt caggtaacag
    12421 gcccacgcgc tccagcaacc acgcaacgcg tgcagccgcg tctttacctg gcaataaacc
    12481 gtgtacacgc agcggttcga taatcgaatc accgatggtc tgacgtgggt ccagcgaagc
    12541 gtaagggtcc tgaaaaataa actgaatatc ccggcgcaat gcctgaagtt tgccaggtga
    12601 caaggtatcg attcgctgac cgttaaagat aatttcgccg ccctgcgatt cgaccaggcg
    12661 cagcaacgcc cgcccggtag tggatttacc gctgccagac tcgcccacca gcgatagcgt
    12721 ttcgccaggc cagagatcaa aactgacttt ctcaacggca tgcacttccc gcgttacgcg
    12781 attcaacaaa ccgctgcgca aagggaaacg ggtgacaaga ttacgcaccc gtaaaacagg
    12841 ttcgccatcc accaccgttt tctgctcgat ggggggttcc tgtttcgctg gatgttcaag
    12901 cgatatcaat gggaaacgtc ggggataatc taaccctttc atcgcaccaa gttgcggaac
    12961 agcagctaac agcgcacggg tgtaaggatg ttgcggtgca tgaaaaatct gttcgacggt
    13021 acccgtttcc accgcctcgc cctgatacat caccagtacc cgatcggcaa tctctgccac
    13081 cacgcccata tcgtgagtga taaagataac gcccatcgac atctcttttt gcaatacttt
    13141 gattaattgc aggatctgcg cctgaatagt gacatccagc gcggtggttg gctcatcggc
    13201 aatcagcacc gccgggcggc atgacagcgc catcgcaatc atcactcgct ggcgcatccc
    13261 gccagagagt tgatgcgaat aacgtgaaag aatggtttgt gcctccggaa tgcgtacctg
    13321 atccagcatc cgcttcgcct cgaccatcgc ttcttcacga ctggcgttct gatgcagacg
    13381 aattgattcg gcaatctgtt cacccacagt aaatactggg ttcagcgatg tcatcggctc
    13441 ctgaaatatc atcgccatat ccgcaccgcg cacatggcgc atttgtgcag cgctctgctc
    13501 gctaagttca atcacatcac gactgcgccg ccgcaacagc attttatcgc actgtactaa
    13561 accgcccgcc tgttccaaca gacgcatcaa tgccagcgca gtcactgact taccggagcc
    13621 ggattcgcct acaattgcca gcgtctcacc gcgttgcaga ctaaaagaga gattgcggac
    13681 cgcagctatt ttctgctggt cctgcataaa ggcaatatta agattttcaa ccgccagcac
    13741 attaccggca tcaagttcat cactgtgtgg caacggtgtc ccctttttca cggtagatac
    13801 cggtggttgg cgtatcgcct gcgtagcccc aggcgcgata cattccttcg gtgttaaacg
    13861 gtagcgcgac attcccttca tggtcgatag cgattaagcc accgctaccg ccaagcgcag
    13921 ggagtttttc cattactacc cgctcgcagg cttccgcgag acttaatccg ccgtaatcca
    13981 ttaacgcggc gatgtcatat gccgccagcg cgcggatgaa gacttcgcct gtgccggtac
    14041 aagaaaccgc cacactggcg ttattggcgt agcatccggc acccactaag ggactatcgc
    14101 caactcgtcc gggtaattta ttggtcattc cgcctgtgga cgtggctgcc gccagattgc
    14161 cgtctaaatc caacgccacg gcccccacgg tgcccatttt ctgtttttca tccagtggcg
    14221 caccgctatg gtcgaggact gttgcccctt cctcgcgcgc tgccagtagt tgttcataac
    14281 gcaaaggcgt ggagaaaatc tccggtgaga cgcgctccat gccatgagca aacgcaaaat
    14341 tttctgcccc ttcgccaatc atcatcacat gcgggctttg ctccatcacc agccgggcgg
    14401 caagaaccgg attacgcaga tgactaacgc ccgccaccgc accggctttc agggtgttac
    14461 catccatcac acaggcgtcc agttcatggg tttcatcacg cgtaaagaca gcgccaattc
    14521 cggcgttaaa cagtggacac tcttccagca gacgcaccgc ttccgtcacc acatccagcg
    14581 cactttcgcc ggcttccagc attttctgcc cggtttcaac aatggcagac aacgcctcga
    14641 tgtagcgtaa ttcctgttgc agactcatct gcgcgcggct aattgcacct gcgccaccat
    14701 gaattgcaat gactgctttg cccatagtat tcgtccatta agggtggctc gccacttttg
    14761 ctataaatat cagaattttt atgaattact cattcaattt ttgaatatag aaagatggat
    14821 atggcatgta aaggcaggag ccaacgctac gcccgaggtg gcatacagtc tatggcgttt
    14881 ttctgacata ataggcaaat tcgattttgc ctccgcagga gtgttttcat ggaatttacc
    14941 accggattga tgtcgctcga caccgcgctt aatgagatgc tttctcgcgt taccccactg
    15001 accacccagg aaacgctgcc actggttcag tgttttggtc gtattctggc gagcgatgtc
    15061 gtttcgccac tggatgtccc ggggtttgat aactccgcta tggacggcta cgcggtgcgt
    15121 ttagccgata ttgcctccgg gcaaccgctg cccgttgccg gtaaatcctt tgccggtcag
    15181 ccataccatg gtgaatggcc tgcgggtacc tgcattcgta ttatgaccgg tgcgccggtg
    15241 ccggaaggct gcgaagcggt ggtgatgcag gagcagactg aacaaacgga caatggcgtg
    15301 cgttttactg ctgaagtgcg tagcgggcaa aatattcgcc gtcgcggtga agatatctct
    15361 gcaggtgcgg ttgttttccc ggcgggaact cgcctgacta ccgcagagct gccagtaatt
    15421 gcttcactgg ggattgccga agttccggtg attcgtaaag tgcgtgtagc gcttttttct
    15481 accggtgatg aactccagtt gcccggtcag ccgctgggcg acggccaaat ctacgacacc
    15541 aaccgtctcg ccgtacacct gatgttagaa cagctgggtt gcgaggtaat caacttaggg
    15601 attatccgcg acgatccaca tgccctgcgc gccgcattta ttgaagccga cagccaggcg
    15661 gatgtagtga tcagttccgg cggtgtttca gtgggtgaag cggattacac caaaatgatt
    15721 cttgaagagc tgggggagat cgccttctgg aagctggcga ttaaaccagg taaaccgttc
    15781 gcgttcggta aactcagcaa tagctggttc tgcggcctgc cgggcaaccc ggtttcagcg
    15841 acgctgacct tctatcaact ggtacagcct ttgctggcaa aactaagcgg caacaccgcc
    15901 agcggcctgc ccgcgcgcca gcgcgtacgc acagcgtccc gcctgaaaaa aacgccagga
    15961 cgtcttgatt tccagcgcgg cgtgctgcaa cgcaacgccg acggtgaact tgaagtgacg
    16021 accaccggac atcagggttc acatatattt agctccttta gtctcggcaa ctgctttatc
    16081 gtgctggaac gcgatcgcgg caatgtggac gtgcgcgaat gggtggaagt agaaccgttt
    16141 aacgcgttgt tcggaggcct gtaatggcgg aactcagcga tcaggagatg ctgcgctaca
    16201 accggcaaat cattctgcgc ggttttgatt ttgacggtca ggaggcgcta aaagattctc
    16261 gtgtgctggt agtcggcctc ggtggactcg gctgcgcagc ttcgcagtat ctggcaagcg
    16321 ccggtgtcgg caacctgacg ctgctcgact tcgacacggt ttcgctctcg aatctgcaac
    16381 gccagacact gcacagtgat gccacggtcg ggcaaccgaa ggtggaatcc gcccgtgacg
    16441 ccctgacgcg gatcaaccca catatcgcga ttacgccagt caatgcactg ctagatgacg
    16501 cagaacttgc agcattgatt gctgaacacg atctggtgct cgactgtacg gataacgttg
    16561 cggtacgtaa tcaactgaac gcaggctgtt ttgccgcgaa ggtgccgctg gtttccggcg
    16621 cggcaattcg tatggaaggg caaatcacgg tcttcactta tcaggacggc gaaccgtgct
    16681 atcgctgcct tagccgtttg tttggcgaga acgcgttaac ctgcgtagaa gcaggcgtga
    16741 tggcaccgtt gataggcgta attggttcgt tgcaggcaat ggaagcgatc aaactgctgg
    16801 caggttatgg caaaccggcc agcgggaaaa tcgtcatgta cgatgcgatg acctgtcagt
    16861 ttcgcgaaat gaaactgatg cgcaatccgg ggtgtgaggt gtgcgggcag taattgccac
    16921 ctccaagcct gataagacgc gacaagcgtc gcatcaggca tttgctccag acgccagatg
    16981 cggcgtgaac gccttatctg gcctacggtt cgggcacaga tttccaagcg cggcaggcat
    17041 cggtgcactc cgccacgtca tcagcaattt atgaggatgc agaacgtccg gttaaatcga
    17101 cgttctgcca aacgctccct gccagtcctg ctcaaacttc gtcacagcgg cttccaccgc
    17161 cggatagcta atcatctgtt gtgccacatc cagtggcaga gtaattgatt cacatccggc
    17221 cagtaagcag tccagcgcct gacgcggggt tttgaaactc gctgccagca ctttcgcctg
    17281 cggcgcatgc attttcaata actggtgtaa gtcggtcaca gtctgaatgc cgctaccgcc
    17341 ctgagcatca atacgattaa cgtaaggcgc aacatattcc gcacctgcca gcgccgacag
    17401 cagcccttgt gccgcgccat ataccgcggt tcccagcgtc ggaatccctt ccgcttttaa
    17461 catcttaatc gctgccagcc cctcggctgt caccggaact ttcaccacga tatccgcaat
    17521 aatagaacgc agcttaagcg cgtcattcac catcccttca gcagtggtag ccattacctg
    17581 ggcaaacaga cgcccctgac cgcccatcgc ttcatgaagt tgcggaagca caacatccag
    17641 cggtttttta cccgcggcga taatgcttgg gttagtggtc acacccgcca gcggaaaaat
    17701 acgtgacagc gccttcaccg caacaacgtc tgaagtatcc agatacagtt ccataaccat
    17761 cctcaaaatg tactttttgt ctgaatgtag actatcacgg gaaaactctc tgaacagttg
    17821 acgcacatca atctaacttt cattcgaaag tattttaatg tttgaacgaa acagagaggc
    17881 aactatgatt ttcaatattc agcgctactc gacccatgat ggccccggta tccgcacggt
    17941 cgtatttctt aaaggctgtt cgctgggctg ccgctggtgt cagaacccgg aaagccgcgc
    18001 ccgtacgcag gatctgctgt atgacgcacg actgtgtctg gaaggctgcg agctgtgcgc
    18061 taaggccgcg ccggaagtga ttgagcgcgc gctgaatggt ttgcttattc atcgggaaaa
    18121 gttaaccccg gagcatctga cggcgttaac cgactgctgt ccgacacagg cgttaaccgt
    18181 gtgtggtgaa gtgaaaagcg ttgaggagat catggcgacc gttctgcgcg ataaaccgtt
    18241 ttacgatcgc agcggcggcg gcttaacgct ttcgggtggt gaacccttca tgcagccgga
    18301 aatggcgacg gcgctgctgc aagcctgcca cgaggcaggc attcatactg cggtagaaac
    18361 ctgtctgcat gtgccgtgga aatatatcgc cccttctctg ccctatatcg atctgtttct
    18421 tgccgattta aaacacgttg ccgacgcgcc gtttaaacag tggaccgacg gtaacgccgc
    18481 cagggtgctg gataacctga aaaaactcgc cgcagcgggc aaaaaaatca ttatccgcgt
    18541 gccgctgatt cagggcttta atgccgacga aacctctgta aaagccatta ccgattttgc
    18601 cgccgacgag ctgcacgttg gcgaaattca ttttctgccc taccacacgc tgggcatcaa
    18661 caaatatcac ttacttaatc tgccctatga cgccccggaa aaaccgcttg atgcgccaga
    18721 gctgctcgat tttgcccagc agtatgcctg ccagaaaggg ttaaccgcga ccttacgagg
    18781 ataacaacca tgaccacact gaaactggac acgctcagcg accgcattaa agcgcacaaa
    18841 aatgcgctgg tgcatattgt gaagccgcca gtctgtaccg agcgcgcgca gcactatacc
    18901 gagatgtatc aacaacatct cgataagccg atcccggtac gtcgcgcgct ggcactggcg
    18961 catcacctgg cgaatcgcac catctggatc aaacacgatg agttgatcat tggcaaccag
    19021 gcaagcgaag ttcgcgccgc gccgatcttc ccggaatata ctgtctcgtg gatcgaaaaa
    19081 gagattgatg atctggcaga tcgtcccggt gctggctttg cggtgagcga agagaacaaa
    19141 cgtgttctgc atgaagtgtg cccgtggtgg cgcggtcaga cggtacagga tcgctgctat
    19201 ggcatgttta ccgatgagca aaaaggtctg ctggcgaccg gcatcattaa agcggaaggc
    19261 aatatgacct ccggcgatgc gcacctggcg gtcaatttcc cgctgctgct ggaaaaaggg
    19321 cttgatggtc tgcgtgagaa agtagcggaa cgtcgctcgc gcatcaatct gacggtgctg
    19381 gaagatttgc acggcgaaca gttcctgaaa gcgattgata tcatgctggt ggcagtcagt
    19441 gaacacattg aacgtttcgc tgccctggcg cgtgcaatgg ccgcgaccga aacccgcgaa
    19501 agccgtcgcg atgaactgct ggcgatggca gaaaactgcg atcttatcgc ccaccagccg
    19561 ccgcagactt tctggcaggc gctgcaactg tgttacttca tccagttgat tttgcagatt
    19621 gaatctaacg gtcactcggt gtcgtttggt cgtatggatc agtatctcta cccgtactat
    19681 cgccgcgacg ttgaactgaa ccagacgctg gatcgcgaac acgccatcga attgctgcat
    19741 agctgctggt tgaaattgct ggaagtgaac aaaatccgct ccggctccca ctcaaaagcc
    19801 tctgcgggaa gtccgctgta tcagaacgtc accattggcg gacaaaatct ggttgatggt
    19861 caaccaatgg acgcggtgaa tccactctct tacgcgatcc tcgaatcctg cggtcgcctg
    19921 cgttccactc agcctaacct cagcgtgcgt taccatgcag gaatgagtaa cgatttcctc
    19981 gacgcctgcg tacatgtgat ccgctgcggc ttcgggatgc cggcattcaa caacgacgaa
    20041 atcgtgatcc cggaatttat taaactcggt attgaaccgc aggacgctta tgactacgca
    20101 gcgattggtt gtatagaaac cgccgtcggt ggcaaatggg gctatcgctg taccggcatg
    20161 agctttatca acttcgcccg cgtgatgctg gcggcgctgg aaggcgggcg tgatgccacc
    20221 agcggcaaag tgttcctgcc acaagaaaaa gcgttgtcgg caggtaactt caacaacttc
    20281 gatgaagtga tggacgcgtg ggatacgcaa atccgttact acacgcgtaa atcaatcgaa
    20341 atcgaatatg tcgtcgacac catgctggaa gagaacgtgc acgatattct ctgctcggcg
    20401 ctggtggatg actgtattga gcgagcgaaa agtatcaagc aaggcggcgc gaaatatgac
    20461 tgggtttctg gcctgcaggt cggtattgcc aacctcggca acagcctggc ggcagtgaag
    20521 aaactggtgt ttgaacaggg cgcgattggt cagcaacagc tggctgccgc actggcggat
    20581 gacttcgatg gcctgactca tgagcaactg cgtcagcggc tgattaacgg tgcgccgaag
    20641 tacggcaacg acgatgatac cgtcgatacg ctgctggctc gcgcttatca gacctatatc
    20701 gacgaactga aacagtacca taacccacgc tacggtcgtg gtccggttgg cggcaactat
    20761 tacgcgggta cgtcgtctat ctccgctaac gtaccgtttg gcgcgcaaac tatggcaacg
    20821 ccggacgggc gtaaagccca caccccgctg gcagaaggcg ctagtccggc ctctggtact
    20881 gaccatctcg gccctactgc ggtcattggc tcagtgggta aactgcctac ggcagcgatt
    20941 ctcggcggcg tgttgctcaa ccagaaactg aatccggcaa cgctggagaa cgaatctgac
    21001 aagcagaaac tgatgatcct gctgcgtacc ttctttgaag tgcataaagg ctggcatatt
    21061 cagtacaaca tcgtttcccg cgaaacgctg ctggaggcga aaaaacatcc ggatcagtac
    21121 cgcgatctgg tagtgcgtgt cgcgggttat tccgccttct tcaccgcgct ctctccagac
    21181 gctcaggacg atatcatcgc ccgtactgaa catatgctgt aatctcttcg gcccggctgc
    21241 ggcgtcgccg ggctaaatca ttcacatcat caatttcatc cttactttca ttcgaaatat
    21301 aatttgtgct ctgcgtcaca ttgttattac actgttacgg tttcattttc gagccaggag
    21361 cacacctatg agcgtaaaag ttatcgtcac agatatggac ggtacttttc ttaacgacgc
    21421 caaaacgtac aaccaaccac gttttatggc gcaatatcag gaactgaaaa agcgcggcat
    21481 taagttcgtt gtcgccagcg gtaatcagta ttaccagctt atttcgttct ttcctgagct
    21541 aaaggatgag atctcttttg tcgcggaaaa cggcgcactg gtttacgaac atggcaagca
    21601 actgttccac ggcgaactga cccgacatga atcgcggatt gttattggcg agttgctaaa
    21661 agataagcaa ctcaattttg tcgcctgcgg tctgcaaagt gcatatgtca gcgaaaatgc
    21721 ccccgaagca tttgtcgcac tgatggcaaa acactaccat cgcctgaaac ctgtaaaaga
    21781 ttatcaggag attgacgacg tactgttcaa gttttcgctc aacctgccgg atgaacaaat
    21841 cccgttagtg atcgacaaac tacacatagc gctggatggc attatgaaac ccgtcaccag
    21901 tggttttggc tttatcgacc tgattattcc cggtctacat aaagcaaacg gtatttcgcg
    21961 gttactgaaa cgctgggatc tgtcaccgca aaatgtggta gcgattggcg acagcggtaa
    22021 cgatgcggag atgctgaaaa tggcgcgtta ttcctttgcg atgggcaatg ctgcggaaaa
    22081 cattaaacaa atcgcccgtt acgctaccga tgataataat catgaaggcg cgctgaatgt
    22141 gattcaggct gtgctggata acacatcccc ttttaacagc tgacctctct accggagcca
    22201 tgcgctccgg ttttcccttc tttttacatc ctgtacatca catcaaatca ttaaacttgc
    22261 attaacttat ttataacttg tattgtcaca tgcaaattag catagtttcg aatgaaagtt
    22321 aatgcaaggt ggttatggct tctactttta ccagcgacac attgcctgcc gatcacaaag
    22381 cagctatccg tcagatgaag cacgcgctgc gggcgcagct tggcgatgtc cagcagatct
    22441 ttaatcagct aagcgatgac attgccacgc gagtggctga aatcaacgca ctcaaagcac
    22501 agggcgatgc cgtctggccg gtgctgtctt atgccgatat caaagcaggt catgttactg
    22561 cagagcagcg cgaacagatt aaacgtcgcg gttgtgcggt gataaaaggc catttccccc
    22621 gcgaacaagc gctaggctgg gatcagtcga tgctggacta tctgaaccgc aaccgctttg
    22681 acgaggtcta caaaggcccc ggcgataatt tcttcgggac gctcagcgct tcacgtcccg
    22741 agatttaccc catctactgg tcgcaggcgc aaatgcaggc ccgccagagt gaagaaatgg
    22801 cgaatgcgca gtcgtttctc aatcgtctgt ggacatttga aagtgatgga aagcaatggt
    22861 ttaacccgga tgtgagcgtc atctaccctg accgtatccg ccgccttccg cccggaacga
    22921 cctccaaagg tcttggagcg cataccgact ccggggcgct ggaacgctgg ctgcttccag
    22981 cgtatcagca cgttttcgcc aacgtcttta atggcaatct ggcgaaatac gatccctggc
    23041 atgcggcaca tcgtacggaa gttgaagagt acacggtgga caacaccacc aaatgttccg
    23101 tgtttcggac attccagggc tggactgcgc tctcggatat gctgcctggt caggggttgc
    23161 tgcacgtcgt gcccattcct gaagccatgg cgtacgtact gttacgtccg ctgcttgatg
    23221 atgtgccgga ggatgaactg tgcggcgtag cgcccggaag agtgttgccg gtatcagagc
    23281 aatggcatcc actgttaatt gaggcgttaa ccagcattcc aaaactcgag gccggagact
    23341 ccgtctggtg gcactgcgac gtcatccatt ccgttgcccc cgttgaaaat caacagggtt
    23401 ggggcaacgt gatgtacatt cctgcggcac cgatgtgcga gaaaaatctt gcctacgcgc
    23461 acaaggtgaa ggccgcactg gaaaaaggcg catcgccggg cgacttcccg cgcgaggact
    23521 atgaaacaaa ctgggaagga cgctttacgc ttgccgacct caacattcac ggtaagcgag
    23581 cgttgggcat ggatgtttga gagttaatgg aagtgaattg atgtgggatt aagaaaatcg
    23641 attcgacgtt actcagattg ctgacaagtg cgctttctta atgcctgatg cggcgttaac
    23701 gccttatccg gcctacaaag tcctgcagat tcaatatatt acaggtactg cgtaggcctg
    23761 ataagcgtag cgcatcaggc tattttgcat ttgtcatcag ccgtcgtcac cccgacagta
    23821 tcggggtgac ggataatcac ttactcgatc cctttactac gcaggtaatc ttcgtaatta
    23881 ccgctaaagt cgatcacgcg ttccggggtg atttccagaa tgcgggtcgc cagggagctt
    23941 acgaactcac ggtcgtgtga aacaaagatc agcgtgccct gatacagttc cagcgccatg
    24001 ttcagtgact cgatggattc catatccagg tggttggtcg gttcgtccat gatcagaatg
    24061 ttcggcttct gcatcattaa cttaccaaac agcatccgcc ctttttcccc accggaaagc
    24121 actttagctg gctttttgat gtcgtcctgg ctgaacagca aacgaccgag aatgctgcgt
    24181 accgcctgct cgtcatcgcc ttcctgcttc cactgactca tccattcgaa cacggtcaga
    24241 tcattttcaa actcatattc gtggtcctgg gcgtagtaac caatgcgcgc gttttcagac
    24301 cattttacgg tgccgctgtc cggttgcaga tcgcccacca gcgttttcag cagcgttgat
    24361 ttaccgacgc cgttggtacc cagtaccgca agtttttcac cgacctccag cagcaggttg
    24421 agatttttaa acagtggacc gttatcaaac cctttggtca gaccttccac ttccagcgcg
    24481 ttacggaaca gtttcttatc ctgttcaaaa cggataaacg ggttctgacg gctggaggct
    24541 ttcacctctt ccagtttgat tttatcaatc tggcgcgcgc gcgaagttgc ctggcgagat
    24601 ttcgaggcgt tggcgctaaa gcggctaacg aaagattgca actcagcaat ctgcgctttc
    24661 ttcttggcgt tatcggccag cagacgttca cgcgcttggg tcgccgccgt catgtactca
    24721 tcgtagttac ccggataaac gcgcagctcg ccgtaatcca gatccgccat gtgggtacag
    24781 accatgttaa ggaagtgacg gtcgtgcgag atgatgatca tggtgctgtc acgctcgttc
    24841 agcacctgtt ccagccagcg aatggtgtcg atgtcgaggt tgttggtcgg ttcgtcgagc
    24901 aggagaatat ccggctcagc aaacagcgcc tgcgccagaa gcacacgcag cttccagcca
    24961 ggagcaactt cactcatcgg gccgtagtgc tgttccactg gaattcccac gccaagcaac
    25021 agttcaccgg cgcgagcttc cgcagagtaa ccgtccattt cgccgtattt cacttccaga
    25081 tcggccactt tatagccgtc ttcttcactc atttccggca aagcatagat gcggtcgcgc
    25141 tcctgcttca cttcccacaa ctctttatgc cccatgatca ccgtatccag cacagtgaac
    25201 tcttcaaagg caaactgatc ctgacgcagc ttaccaatgc gctcgttggg atcgagggaa
    25261 acgttaccca gcgtcggctc aaggtcgccg ccgagaatct tcataaaggt ggatttacca
    25321 ctaccgttcg cgccaatcag gccgtaacgg ttgccgccgc cgaatttgac ggaaatgttt
    25381 tcaaacaacg gcttactgcc gaactgcatg gtgacgttac tggaaactaa cacaggcgta
    25441 tcctgaaaag agatatgaca aaccgcgcat tatgccataa gtctgaaaga agatcctgct
    25501 cctcgcttaa ggtctacact caagccaaag gcaaaaaatg tgatttcgta cacatctgat
    25561 ttcactgtga gctggaatga acttataatg cgcttccaat actctaatat tctcaaccca
    25621 atggcctgcc aggcacaaaa tctcgcttaa catgaatatg aaattgaaaa cattattcgc
    25681 agcggccttc gctgttgtcg gcttttgcag taccgcctct gcggtaactt atcctctgcc
    25741 aaccgacggg agtcgcctgg ttggtcagaa tcaggtgatc accattcctg aaggtaacac
    25801 tcagccgctg gagtattttg ccgcggagta ccagatgggg ctttccaata tgatggaagc
    25861 gaacccgggt gtggatacct tcctgccgaa aggcggtact gtactgaaca ttccgcagca
    25921 gctgatcctg ccggataccg ttcatgaagg catcgtcatt aacagtgcag agatgcgtct
    25981 gtattactat ccgaaaggga ccaacaccgt tatcgtgctg ccgatcggca ttggtcagtt
    26041 aggcaaagat acgcctatca actggaccac caaagttgag cataagaaag caggcccgac
    26101 ctggacgccg accgccaaaa tgcacgcaga gtaccgcgct gcgggcgaac cgctaccggc
    26161 tgtcgttccg gcaggtccgg ataacccgat gggtctgtat gcactctaca tcggtcgcct
    26221 gtatgctatc catggcacca acgccaactt cggtatcggc ctgcgtgtaa gtcatggttg
    26281 tgtgcgtctg cgtaacgaag acatcaaatt cctgttcgag aaagtaccgg tcggtacccg
    26341 cgtacagttt attgatgagc cggtaaaagc gaccaccgag ccagacggca gccgttatat
    26401 tgaagtccat aacccgctgt ctaccaccga agcccagttt gaaggtcagg aaatcgtgcc
    26461 aattaccctg acgaagagcg tgcagacagt gaccggtcag ccagatgttg accaggttgt
    26521 tcttgatgaa gcgatcaaaa atcgttccgg gatgccggtt cgcctgaatt aatcgtcaaa
    26581 acgtaaaaca aaggcggact cataatccgc cttttttatt tgccagacct taccaggccg
    26641 ggatcataac taacaaaacg tatcccacca gcgccgccca caacagcatc ggtattgaat
    26701 agagatggaa acgccaccag atgcggcgat cgttcgccat acgtagcgca atcaaatttg
    26761 ccagcgatcc tggtaataaa ccaaagcccc ccacgtttac cgcccatgcc agtaataaag
    26821 acggaggtac ataattgagc agcaaaatgg tactcggcac gttactgatc acctgcgata
    26881 aaccgactgc cgttaaccat aacccgggtt cagataaatg actcacgtta cccaacacgc
    26941 cttgcaacgc tggaagctgg gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca
    27001 gcagcaacgt ccagtccaca ctcaacacca cgcgacgcgc cagcagcaca aagcccgccg
    27061 ccacaatcac cagtccccac agctcttgtt tgaactccag cgccgtcaga aagacgatat
    27121 acagccccag acaattccac accagccgcg gtttccactc cggtgtttgc acccccgtat
    27181 ggtattgcag cgcctttcca gggaaacaac accagcacaa cagcaggagt gtcagcatca
    27241 ttgcgccagc catcggtgcc atttgggcaa taaatccggc aaacgaaaga ccagaacgtc
    27301 cccagataag aatattttgc gggttgccaa ttggcgtcag tagcgaacca gcgttgactg
    27361 ccagcgcctc aaaaataatc agccgattaa ccgggatctc acacagtctt tttagcgtga
    27421 tagtcagcgg aacaacaata aacagcgcga catcgttggt cagaaaggta gaaagcagcg
    27481 ccgccgccag caccataaac atcgccagcc gacgctccgt agcaaagcgg cgcaccattt
    27541 tgcgccccag cacatcaaaa taaccgctta actccacacc tttggtcagc agcatcaggc
    27601 cgcttaaggt gatgatggtg tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg
    27661 gcacgaaaaa gcttaatccg ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt
    27721 gcagcgtgcg taaaaaaggc aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa
    27781 agcgtcgagc gtctcttcac taacatggtg ctccatgcct tccgcgtcgc gacgggcgat
    27841 ttccggactg acgcccaaca ccaacaagaa attttcgact atctgatgtc gctcgcggct
    27901 ttcctgcgcc agcttctctc cttctgccgt taaaaacacg ccacgccagg ggatcatttc
    27961 aatcagcccc atggttgcca gccgcttaag cattttagcc accgtcggtt gcgaaactcc
    28021 cagacgagca gccatgtcca cctgacgagc ttcccccact tccctgatca agtcagaaat
    28081 cagctcaacg taatcatcaa taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc
    28141 ttcaacgtgc tcttccacgt tcactaactg cgtcactttt tttgctattg gcgtacctgc
    28201 gcgacgactc attgtgcttc ctcattatgg tgacgcatac atagcgttct catttcgtgc
    28261 gcacattgta aaccagagtt gcgaaggtac aaaaaattaa cgttttagca atagctatat
    28321 aatatagcct gtactatatc tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta
    28381 gcaagtcagg aggtcttatg aatgagttca agaggtgtat gcgcgtgttt agtcattctc
    28441 cctttaaagt acggttaatg ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc
    28501 agcaagataa accttcagat aaatagcggc gtcgcggtac gccgcttcac tcctgctttt
    28561 atgcaggcat aacgcgtttt ggtctgaaaa aaaccacttt ttgtcggatt tgcaatcccc
    28621 ttcgcaaaag atttgttcgt cagtagttga cctgaacggc ggctcgctct atcttcttgc
    28681 agccctgcgt atattgcggc tcgcggatgc ggaccccttt cccctcttca cgcactcttg
    28741 caggtattga cccttgacgc cagggtaagc acatggcgtt tgttacgata gtggcatatg
    28801 aatttaaccc tcaaagaatc gcttgttacc cgtagccggg tatttagccc gtggactgcg
    28861 ttctactttt tacagtcgct attaattaac ctcggcttag gttacccctt cagtttgctc
    28921 tacaccgctg cgtttacggc tattttgctt ttgctatggc gaacattgcc tcgcgtacaa
    28981 aaagttctgg tcggtgtcag ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc
    29041 tacggcgcgc ctaatttcaa tacattgctg gcattgcact ccaccaatat ggaagagtcg
    29101 accgaaatcc tgacgatttt tccgtggtac agctacctgg tcggcttatt tatttttgcg
    29161 ctcggcgtaa tagcaatcag gcgaaaaaaa gagaatgaaa aagcgcgctg gaataccttc
    29221 gacagcctgt gtctggtatt cagtgtggcg acattttttg ttgctcccgt gcaaaacctg
    29281 gcctggggtg gcgtatttaa actgaaagat actggctatc cggtatttcg ttttgctaag
    29341 gatgtcatcg tcaataataa cgaggtgatt gaagagcaag aacggatggc aaaactttcc
    29401 ggaatgaaag atacctggac ggtcactgcc gttaagccga agtatcagac ctatgtggta
    29461 gtgatcggtg aaagcgcgcg tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat
    29521 accccgtttg ccagcagcgt taacggtttg atatttgctg actacattgc tgccagtggc
    29581 tccacgcaga aatcgcttgg cttaacgctc aatcgcgtag tcgatggcaa accacagttt
    29641 caggataact ttgtcaccct ggcaaatcgc gcgggcttcc agacctggtg gttttccaac
    29701 cagggccaaa tcggcgaata cgatactgct atcgccagca tcgccaaacg tgcagatgaa
    29761 gtgtacttcc tgaaagaagg taattttgaa gcagataaaa acacgaaaga cgaagcgtta
    29821 ctggatatga ccgctcaagt gctggcgcaa gagcactcgc aaccgcagct gattgttctg
    29881 catctgatgg gctcgcatcc gcaggcctgc gacaggacac aaggcaaata cgaaaccttt
    29941 gtgcaatcga aagaaacgtc gtgctatctc tataccatga cgcaaacgga cgatttactg
    30001 cgcaagctgt acgatcagtt acgcaacagc ggcagcagct tctctctggt ttacttttct
    30061 gaccacggtc tggcctttaa agagcgcggc aaagacgtgc aataccttgc ccatgatgat
    30121 aaatatcagc aaaatttcca ggtgcctttt atggtcattt ccagcggcga taaagcgcat
    30181 cgggtgatta aagcccgccg ctcagccaat gacttcttag gctttttctc gcagtggacg
    30241 ggaattaaag cgaaggaaat aaatatcaaa tacccgttta tatctgagaa gaaagccggg
    30301 ccgatataca tcaccaactt ccagttacag aaggtggatt acaaccatct cggaaccgat
    30361 attttcgacc cgaaacctta aaaacaaaaa tccgccccga gaggcggatt tttatatcac
    30421 caaagtgatt agaagcggta accaacaccg gcaatccagg tgcctacgtc aacgctacga
    30481 atacggctct gctcgtaaga gaagtccaga gcaacgtttt ccatcgggtt gaactgcaga
    30541 ccagcaccgt aggagaaacc gtagtcgctg gtgtcgtgtt tgtaggtcgg gtattcagtg
    30601 gtctggaatt taccataacc cacacccact acaccgtaga tgcttgccca gtcgttaatg
    30661 cggtaagccg gaccagcagt gatgccgtag tactggtttt tgttgtagtc accagagctt
    30721 gcagtacggc ttttctcggt gtaagtgaaa gaaccgatca cacccagcgg gctgttgtct
    30781 tcttcatagc ggtatttcag gttgaaaccg cccattttgt tcatttggcc ctgagcgtcg
    30841 ctctgtgcgt aaccgccagt tacggtagaa gtcgcagcta cggaagtacc tgcggtgaaa
    30901 gccagaactg cggccagtgc tgaaagacat gcaatttttt tcataaccac ctcaaatgtg
    30961 attcaaataa gtcctaagtt ttaaatatat caaaaattaa tgggaaactc ttcgcgattt
    31021 gtgatgtcta acgggccatt tcatgtaaca gaacgtttcc atacaccgct atccatctaa
    31081 atttaaatca ctttttcaga gaactgcgta agtattacgc atgttttccc tgtcattcat
    31141 ccagattatt cctaatcacc agactaatga ttccatcaat cctggcgcat tttagtcaaa
    31201 acgggggaaa attttttcaa caaatgctca accagcattg ggtatatcca gtacactcca
    31261 cgctttactt aagtctagat atttgtggga gaaaggatgc ctggttcatt acgtaaaatg
    31321 ccggtctggt taccaatagt catattgctc gttgccatgg cgtctattca gggtggagcc
    31381 tcgttagcta agtcactttt tcctctggtg agcgcaccgg gtgtcactgc gctgcgtctg
    31441 gcattaggaa cgctgatcct catcgcgttc tttaagccat ggcgactgcg ctttgccaaa
    31501 gagcaacggt taccgctgtt gttttacggc gtttcgctgg gtgggatgaa ttatcttttt
    31561 tatctttcta ttcagacagt accgctgggt attgcggtgg cgctggagtt caccggacca
    31621 ctggcggtgg cgctgttctc ttctcgtcgc ccggtagatt tcgtctgggt tgtgctggcg
    31681 gttcttggtc tgtggttcct gctaccgctg gggcaagacg tttcccatgt cgatttaacc
    31741 ggctgtgcgc tggcactggg ggccggggct tgttgggcta tttacatttt aagtgggcaa
    31801 cgcgcaggag cggaacatgg ccctgcgacg gtagcaattg gttcgttgat tgcagcgtta
    31861 attttcgtgc caattggagc gcttcaggca ggtgaagcac tctggcactg gtcggttatt
    31921 ccattgggtc tggctgtcgt tattctctcg accgctctgc cttattcgct ggaaatgatt
    31981 gccctcaccc gtttgccaac acggacattt ggtacgctga tgagcatgga accggcgctg
    32041 gctgccgttt ccgggatgat tttcctcgga gaaacactga cacccataca gctgctggcg
    32101 ctcggcgcta tcatcgccgc ttcaatgggg tctacgctga cagtacgcaa agagagcaaa
    32161 ataaaagaat tagacattaa ttaaatttac atttctgtat ggttatgcat aaccatgcag
    32221 aatttctcgc tacttttcct ctacaccgta tttatatatc gaattatgca aaagcatatt
    32281 tattccgaaa attcctggcg agcagataaa taagaattgt tcttatcaat atatctaact
    32341 cattgaatct ttattagttt tgtttttcat gcttgttacc actattagtg tgataggaac
    32401 agccagaata gcggaacaca tagccggtgc tatacttagt ctcgttaatt actgggacat
    32461 aacatcaaga ggatatgaaa ttatgagtac cgctaaatta gttaaatcaa aagcgaccaa
    32521 tctgctttat acccgcaacg atgtctccga cagcgagaaa aaagcaacag tagagttgct
    32581 gaatcgccag gttatccagt ttattgatct ttctttgatt accaaacaag cgcactggaa
    32641 catgcgcggc gctaacttca ttgccgtaca tgaaatgctg gatggcttcc gcaccgcact
    32701 gatcgatcat ctggatacca tggcagaacg tgccgtgcag ctgggcggtg tagctctggg
    32761 gaccactcaa gttatcaaca gcaaaacccc gctgaaaagt tacccgctgg acatccacaa
    32821 cgttcaggat cacctgaaag aactggctga ccgttacgca atcgtcgcta atgacgtacg
    32881 caaagcgatt ggcgaagcga aagatgacga caccgcagat atcctgaccg ccgcgtctcg
    32941 cgacctggat aaattcctgt ggtttatcga gtctaacatc gaataaatcc atcgctgatg
    33001 gtgcagaact ttagtacccg ataaaagcgg cttcctgaca ggaggccgtt ttgttttgca
    33061 gcccacctca acgcacttat ttagtgcatc catctgctat ccccagctga ttaagtaaat
    33121 tttttgtatc cacatcatca cacaatcgtt acataaagat tgttttttca tcaggtttta
    33181 cgctaaataa tcactgtgtt gagtgcacaa ttttagcgca ccagattggt gccccagaat
    33241 ggtgcatctt cagggtattg ccctataaat cgtgcatcac gtttttgccg catctcgaaa
    33301 aatcaatgag ttgcaaaatt ggcacgattt tttcatatat gtgaatgtca cgcaggggat
    33361 cgtcccgtgg atagaaaaaa ggaaatgcta tgaagtctgt attaaaagtt tcactggctg
    33421 cactgaccct ggcttttgcg gtttcttctc atgccgcgga taaaaaatta gttgtcgcga
    33481 cggataccgc cttcgttccg tttgaattta aacagggcga taaatatgtg ggctttgacg
    33541 ttgatctgtg ggctgccatc gctaaagagc tgaagctgga ttacgaactg aagccgatgg
    33601 atttcagtgg gatcattccg gcactgcaaa ccaaaaacgt cgatctggcg ctggcgggca
    33661 ttaccatcac cgacgagcgt aaaaaagcga tcgatttctc tgacggctac tacaaaagcg
    33721 gcctgttagt gatggtgaaa gctaacaata acgatgtgaa aagcgtgaaa gatctcgacg
    33781 ggaaagtggt tgctgtgaag agcggtactg gctccgttga ttacgcgaaa gcaaacatca
    33841 aaactaaaga tctgcgtcag ttcccgaaca tcgataacgc ctatatggaa ctgggcacca
    33901 accgcgcaga cgccgttctg cacgatacgc caaacattct gtacttcatc aaaaccgccg
    33961 gtaacggtca gttcaaagcg gtaggtgact ctctggaagc gcagcaatac ggtattgcgt
    34021 tcccgaaagg tagcgacgag ctgcgtgaca aagtcaacgg cgcgttgaaa accctgcgcg
    34081 agaacggaac ttacaacgaa atctacaaaa aatggttcag tactgaaccg aaataataac
    34141 gctacacctg taaagcgcac tggcagttcc ctctcccccc tggggagagg attagggtga
    34201 ggggcgcaaa cccgctccgg ggccattaat taccctgaat ttgattattt acaccacggt
    34261 aacaggaaca acatatgcag tttgactgga gtgccatctg gcctgccatt ccgcttctga
    34321 ttgaaggtgc caaaatgacc ctgtggattt cggtcctcgg tctggctggc ggtctggtaa
    34381 tcggattgct ggcaggtttt gcacgcacct tcggtggttg gatagccaac cacgtcgcgc
    34441 tggtctttat tgaagtgatc cgcggcacac ctatcgtcgt ccaggtgatg tttatctatt
    34501 tcgccctgcc gatggcgttt aacgacttac gcatcgaccc atttactgcg gcggtggtca
    34561 ccatcatgat caactccggc gcgtacattg cggaaatcac gcgtggtgcg gtgctgtcta
    34621 tccacaaagg ttttcgtgaa gcaggactgg cgctcggtct ttcacgttgg gaaaccattc
    34681 gctacgtcat tttaccgctg gcactgcgtc gtatgctgcc gccgctgggt aaccagtgga
    34741 tcatcagcat taaagacacc tcgctgttta ttgttatcgg cgtggcggaa ctgacccgtc
    34801 aggggcaaga aattattgcc ggtaacttcc gcgcccttga gatctggagc gccgtggcgg
    34861 tgttctatct gattattacc ctggtgctga gctttattct gcgtcgtctg gaaagaagga
    34921 tgaaaatcct gtgattgaat ttaaaaacgt ctccaagcac tttggcccaa cccaggtgct
    34981 gcacaatatc gatttgaaca ttgcccaggg cgaagtcgtg gtgattatcg ggccgtccgg
    35041 ttccggtaaa tcgaccctgc tgcgctgcat caacaaactg gaagaaatca cctccggcga
    35101 tctgattgtc gatggcctga aggtcaacga tccgaaagtt gacgagcgcc tgattcgcca
    35161 ggaagcaggt atggtgttcc agcagtttta cctcttcccg catctgacgg cgctgggaaa
    35221 cgtcatgttt ggtccgctac gcgtgcgtgg cgcgaacaaa gaagaggcgg aaaaactggc
    35281 gcgtgagctg ctggcgaaag tcggtctggc agaacgtgca catcactacc cttccgaact
    35341 ttctggtggt caacagcagc gtgtggcgat tgcccgcgcg ctggcggtga agccgaaaat
    35401 gatgctgttt gatgaaccga cttccgctct tgacccggaa ctgcgccatg aagtgctgaa
    35461 ggttatgcag gatctggctg aagaagggat gacgatggtg atcgtgaccc acgaaatcgg
    35521 ttttgccgag aaagtagctt cgcggctgat ctttatcgac aaaggccgga ttgcggaaga
    35581 tggcaatccg caggtgttga tcaagaaccc gccgagccag cgcttgcagg aatttttgca
    35641 gcacgtctct taataagaca cattgcctga tgcgctacgc ttatcaggcc tacaggatat
    35701 ctggcaactt attaaaattg catgaacttg taggacggat aaggcgttca cgccgcatcc
    35761 ggcaaaaaag cccgcacgtt gtcagcaacc tgcttaatat cccttcctcc ctttcacccg
    35821 aaagggaggc acaccagatt cctctcattt aaaatcgccc ctcccccagc atctatactt
    35881 atctttttgc tctgttttct cactggagga gtcatgcggt ggatcctgtt catcctcttc
    35941 tgcctgctgg gcgcacctgc ccacgcggta tccatacccg gcgttacaac cacaacgaca
    36001 acggactcaa cgactgaacc ggccccggaa ccggatatcg aacaaaaaaa gcggcctatg
    36061 gcgcactggc ggatgtgctg gataacgaca cctcgcgtaa agagttgatc gaccagttgc
    36121 gtactgtcgc cgcgacgccc cctgcggaac cggtcccgaa gatcgtgccg ccgacgctgg
    36181 ttgaagagca aaccgtgctg caaaaggtca ccgaagtcag ccgccattat ggtgaagccc
    36241 tttccgcccg cttcgggcaa ctttatcgca atatcaccgg ctccccgcat aagccattta
    36301 atccacaaac cttcagcaat gcgctgagcc atttttcaat gttagcggta ttagtgtttg
    36361 gtttttactg gctgattcgc ctgtgcgcac tgccgctata tcgcaaaatg ggtcagtggg
    36421 cgaggcaaaa aaatcgtgag cgcagcaact ggttgcagct tccggcgatg attattgggg
    36481 cgtttattat cgacctgctg ttactggccc tgacattatt tgtcggccag gtattaagcg
    36541 acaacctgaa tgcaggcagt cgcaccatcg ctttccaaca aagtttgttt ctcaacgcct
    36601 ttgccctgat tgaatttttc aaagccgtac tacgcctgat tttttgccca aacgtggcgg
    36661 agctgcgccc gttcacgatt caggacgaga gcgcccgtta ctggggtcgt cgcctgagct
    36721 ggttaagcag cctgattggc tatggcctga ttgtggccgt gccgattatc tctaatcagg
    36781 tgaatgtaca gataggtgcg ctggcgaacg tcatcattat gctgtgcatg accgtctggg
    36841 cgttgtacct gatcttccga aataaaaaag agattaccca gcatatgctc aactttgcgg
    36901 agcattcgct ggcttttttc agcctgttta tccgcgcctt tgcgctggtg tggcactggc
    36961 tggcaagcgc ctattttatc atgctgtttt tcttttcgtt gttcgatccg ggcaacagtc
    37021 tgaaatttat gatgggtgca acggtgcgca gcctggcgat tattggtatc gcagcgtttg
    37081 tttccggtat gttttcccgc tggctggcga aaaccatcac cctctcgcca catactcagc
    37141 gtaactatcc ggagctacaa aaacggctga atggctggct ttcggcggcg ctgaaaacgg
    37201 cgcgtattct gaccgtctgc gtggcagtaa tgttgctgtt gagcgcatgg ggattgttcg
    37261 atttctggaa ctggctgcaa aacggcgcgg ggcagaaaac cgtagatatc ctgatccgca
    37321 tcgcactcat tcttttcttc tcggcggttg gctggacggt gctcgccagt ttgatcgaaa
    37381 accggctggc ttcggatatt catggccgcc cgctacccag cgcccgtacg cgtaccctgc
    37441 tgacgctgtt ccgtaacgcg ctggcggtga ttatcagtac catcaccatc atgattgtgt
    37501 tgtcggaaat cggcgtcaat atcgcgccat tgctggcagg tgccggggca ttaggtctgg
    37561 ctatctcgtt tggttcgcaa acgctggtaa aagatattat caccggggta tttattcagt
    37621 ttgaaaacgg catgaacact ggagatttgg tgactatcgg gccgttgacc ggcacagtgg
    37681 aacggatgtc gattcgctcc gtgggcgtgc gccaggatac cggggcgtat cacatcattc
    37741 cgtggtcttc gataaccacc tttgctaact tcgtccgcgg tattggttcg gtagtggcaa
    37801 actacgatgt tgatcgccat gaagatgctg ataaagccaa tcaggcactg aaagatgcgg
    37861 taacggaatt gatggaaaac gaagaaattc gcgggctgat tattggtgaa ccgaactttg
    37921 ccgggattgt cggcttaagc aataccgcgt ttacactgcg tgtttcgttc accacgctgc
    37981 cactcaaaca gtggacggta cgctttgccc tcgacagcca ggtgaaaaaa catttcgacc
    38041 tggcgggcgt tcgcgcgcca gtgcagactt atcaggtgct gcctgctccg ggcgcgaccc
    38101 cggctgaacc gctgccgccg ggggagccaa cgctttaacg ttggcgatta acaaaacggc
    38161 ggcgctgctc gtcgttcata aaggtccagg caataaagcg actctgcttt tgcccctggg
    38221 ccatctcttt ttttaccacc ttcaccgcgc ccacgtcggt cagagcacga tacaacggcg
    38281 gtaagttttc accacgagat accagcgagg taaaccacat cacctgcttc gcgaagcctt
    38341 tgctctcttc aatcatcttt ttgataaagt tgacttcacc gccttcacac cacaactcct
    38401 gttgctgacc accaaagttc agtgcatcgt ctttgttcag ccccaggtta cggcgtttac
    38461 gctcactacc tgcccgcgcc gcagcggcgg aatcgtggaa tggcgggtta cacaaggtcg
    38521 cgtcgtattg ctcgtttttg tggatgatgc cgttaaaaat cgccccactc tctttttgcc
    38581 gacgcagacg aatggcgcgg ttaagccccg gattagcact gataatcgcc tgcgcactgc
    38641 ttaacgcctg gctgctggtt tcactaccgg taaaacgcca gccatattca tgtacgccaa
    38701 tcagcggata aatacagttc gcaccaacgc cgatatccag aatgctggca ttcgctggaa
    38761 ttgttccgct tgcttcagcc agtaaatcgg caaggtgatg aatataatcc gcccggcccg
    38821 gtactggtgg gcagagaaaa ccgtcgggga tatcccagtt cgctacggcg taaaaatggg
    38881 ccagcaacgc cttattgagc gccttcaccg ccagcggatt ggcaaagtct acgctttgct
    38941 cccctgcggg tgtaagcgtg aggaattgcc tgagttcagg attgacctga caaagcgtgg
    39001 cgagatcgta gcggctgtga tgacggttgc gcggatgcaa ccccggtttc tgggcggaca
    39061 tagcattctc ctttaagcag cggcgtaaga tacccgcata acgcgacagg gtaaatatct
    39121 ggctgcgttt atcattcccc aaaaccttaa catggctatg gtttatggca attcatcata
    39181 aaaatcagca ttatgcatgt cattgatttg tcttattttg taaaaatata tcgttcaaaa
    39241 aataagcatt tctctgtcaa ttttttgcgt catttgcatc gcgcaggcga cctacactta
    39301 tcaggcacta cccgttgtat ttatgaggat gtcattatga aaaagtgcct cacactactg
    39361 attgccaccg tcctgagcgg aatctctctc acggcttatg ccgcgcaacc gatgagtaac
    39421 ctggacagcg gtcagctacg gcccgccggt accgtttcgg caactggcgc atcaaaccta
    39481 agcgatctgg aggataaact ggcagaaaaa gcgcgcgaac aaggcgcgaa aggttatgtc
    39541 attaattccg caggcggaaa tgaccagatg ttaggtactg caaccatcta caaataaccc
    39601 caccaacgta actgcattcg cctctggatg cagttttcgc ctgttttgta atcaaaaaac
    39661 atcaaatatg aacgcaatgt aaataaatgt atttcttttt cgcgcaatgg gtgatagaaa
    39721 atcgcatcaa gtgataatgc ttatcaaaat tattatcact tgcacgaaca ctatcacggg
    39781 attaacagtg gcatcgcatc cgcagagatg ctttctcgtg gcagtgaaaa tttcaacata
    39841 taagaaaaag tcacctgcaa aatggaaaac aatcgcaatt tccctgccag acaatttcat
    39901 tcgctcacgt tctttgccgg tctttgtatt ggtattacgc ctgtggcaca ggcactcgcc
    39961 gccgaagggc aagctaacgc ggatgacacg ctggttgtcg aagcatcaac gccttcgctt
    40021 tatgcgccac aacaatctgc cgatccgaaa ttctcgcgtc cggtagcgga tactacccgc
    40081 acgatgacgg tgatttctga acaagtgatt aaagatcagg gcgcaaccaa ccttaccgac
    40141 gcgctcaaaa ac
//
LOCUS       DAJTUI010000027        39999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTUI010000027 DAJTUI010000000
VERSION     DAJTUI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.027"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..155
                     /locus_tag="OO464_001553"
     CDS             <1..155
                     /locus_tag="OO464_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6226745.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="OO464_001554"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="OO464_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6226746.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="OO464_001555"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="OO464_001555"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6226747.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="OO464_001556"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="OO464_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="OO464_001557"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="OO464_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6226748.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="OO464_001558"
     CDS             complement(5626..7134)
                     /locus_tag="OO464_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6226749.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OO464_001559"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OO464_001559"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6226750.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="OO464_001560"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="OO464_001560"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6226751.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="OO464_001561"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="OO464_001561"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6226752.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OO464_001562"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OO464_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6226753.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OO464_001563"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OO464_001563"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR6226754.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="OO464_001564"
     CDS             complement(14283..15602)
                     /locus_tag="OO464_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR6226755.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OO464_001565"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OO464_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6226756.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="OO464_001566"
     CDS             16699..17034
                     /locus_tag="OO464_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR6226757.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OO464_001567"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OO464_001567"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6226758.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="OO464_001568"
     CDS             complement(17790..19172)
                     /locus_tag="OO464_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6226759.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /locus_tag="OO464_001569"
     CDS             complement(19358..20302)
                     /locus_tag="OO464_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6226760.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="OO464_001570"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="OO464_001570"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6226761.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="OO464_001571"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="OO464_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6226762.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OO464_001572"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OO464_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6226763.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="OO464_001573"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="OO464_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6226764.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="OO464_001574"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="OO464_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6226765.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="OO464_001575"
     CDS             complement(25947..26768)
                     /locus_tag="OO464_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6226766.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="OO464_001576"
     CDS             complement(26868..26951)
                     /locus_tag="OO464_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226767.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27379)
                     /locus_tag="OO464_001577"
     CDS             complement(27044..27379)
                     /locus_tag="OO464_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6226768.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27776..29029)
                     /locus_tag="OO464_001578"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="OO464_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30056
                     /locus_tag="OO464_001579"
     CDS             29136..30056
                     /locus_tag="OO464_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6226769.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAELAKEPFVFFDPHVGTGLYDDI
                     LGLMRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDA
                     VSEMWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30191..31411
                     /gene="mlc"
                     /locus_tag="OO464_001580"
                     /gene_synonym="dgsA"
     CDS             30191..31411
                     /gene="mlc"
                     /locus_tag="OO464_001580"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6226770.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31536..32231
                     /gene="bioD"
                     /locus_tag="OO464_001581"
     CDS             31536..32231
                     /gene="bioD"
                     /locus_tag="OO464_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6226771.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32184..33476)
                     /gene="clcB"
                     /locus_tag="OO464_001582"
     CDS             complement(32184..33476)
                     /gene="clcB"
                     /locus_tag="OO464_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR6226772.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33635..34249)
                     /gene="dmsD"
                     /locus_tag="OO464_001583"
     CDS             complement(33635..34249)
                     /gene="dmsD"
                     /locus_tag="OO464_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6226773.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34292..35146)
                     /locus_tag="OO464_001584"
     CDS             complement(34292..35146)
                     /locus_tag="OO464_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6226774.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35148..35765)
                     /gene="dmsB"
                     /locus_tag="OO464_001585"
     CDS             complement(35148..35765)
                     /gene="dmsB"
                     /locus_tag="OO464_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6226775.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35776..37814)
                     /locus_tag="OO464_001586"
                     /pseudo
     CDS             complement(35776..37814)
                     /locus_tag="OO464_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37875..38984)
                     /locus_tag="OO464_001587"
     CDS             complement(37875..38984)
                     /locus_tag="OO464_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6226776.1"
                     /translation="MINVSHIRTLSSIESTTSSPSIRLAMESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38967..>39999
                     /locus_tag="OO464_001588"
     CDS             38967..>39999
                     /locus_tag="OO464_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6226777.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVES"
BASE COUNT        10165 a         9702 c        10246 g         9886 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa gcaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggctgaactg gctgaactgg cgaaagaacc ctttgtcttt tttgatccgc
    29761 acgtcgggac tgggctgtat gacgatattc tcggactgat gcgacgttac catttgacgc
    29821 ccgtcatcac tcaggaggtg ggcgaggcaa tgaccatcat cggtctggtt tccgccggtc
    29881 tgggtgtttc aattttgcct gcgtcattta aacgtgttca gctcaacgaa atgcgctggg
    29941 tgccgattgc tgaagaggat gcggtttctg aaatgtggtt ggtctggccg aaacatcatg
    30001 aacaaagtcc agctgcgcgt aactttcgta ttcatctgct gaatgctctc aggtgaggga
    30061 aatttcagcg aaaaagcccg aaaaatgtgc tgttaatcac atgcctaagt aaaaatttga
    30121 cgacacgtat tgaagtgctt caccatagcc tacagattat ttcggagcgc gaaaatatag
    30181 ggagtatgcg gtggttgctg aaaaccagcc tgggcacatt gatcaaataa agcagaccaa
    30241 cgcgggcgcg gtttatcgcc tgattgatca gcttggtcca gtctcgcgta tcgatctttc
    30301 ccgtctggcg cagctggctc ctgccagtat cactaaaatt gtccgtgaga tgctcgaagc
    30361 acacctggtg cgagagctgg aaatcaaaga agcggggaac cgtggccgtc cggcggtggg
    30421 gctggtggtt gaaactgaag cctggcacta tctttctctg cgcattagtc gcggggagat
    30481 tttccttgct ctgcgcgatc tgagcagcaa actggtggtg gaagagtcgc aggaactggc
    30541 gttaaaagat gacttgccat tgctggatcg tatcatttcc catatcgatc agttttttat
    30601 ccgccaccag aaaaaacttg agcgtctaac ttcgattgcc ataaccctgc cgggaattat
    30661 tgatacggaa aatggtattg tacatcgcat gccgttctac gaggatgtaa aagagatgcc
    30721 gctcggcgag gcgctggagc agcataccgg cgtaccggtt tatattcagc atgatatcag
    30781 cgcatggacg atggcagagg ccttgtttgg tgcctcacgc ggggcgcgcg atgtgattca
    30841 ggtggttatc gatcacaacg tgggggtggg cgtcattacc gatggtcatc tgctacacgc
    30901 aggcagcagt agtctcgtgg aaataggcca cacacaggtc gacccatatg gtaaacgctg
    30961 ttattgcggg aaccacggct gcctcgaaac catcgccagt gtggacagta ttcttgagct
    31021 ggcacagctg cgtctcaatc aatccatgag ctcgatgtta catgggcaac cgttaaccgt
    31081 ggactcattg tgtcaggcgg cattgcgcgg cgatctactg gcaaaagaca tcattatcgg
    31141 ggtgggcgcg catgtagggc gcattcttgc catcatggtg aatttattta acccacaaaa
    31201 aatactgatt ggctcaccgt taagtaaagc ggcagatatc ctcttcccgg tcatctcaga
    31261 cagcatccgt cagcaggccc ttcctgcgta tagtcagcac attagcgttg agagtactca
    31321 gttttctaac cagggcacga tggcaggcgc tgcactggta aaagacgcga tgtataacgg
    31381 ttctttgttg attcgtctgt tgcagggtta acatttttta actgttctac caaaatttgc
    31441 gctatctcaa tttgggccag gaaagcataa cttagacttt caaggttaat tattttccta
    31501 gtttatattt gtgaagcata acggtggagt tagtgatgct gaagcgtttc tttattaccg
    31561 gtacagacac ttctgtaggg aaaacggtgg tttcccgcgc attgctacaa gcgttagcct
    31621 cccagggaaa aagggttgcg ggatataaac ccgtagcgaa ggggagcaaa gagacacccg
    31681 aagggctgcg taataaagat gccttggtgt tgcagagtgt ttcaaccatc gaactgcctt
    31741 atgaagcagt taatcctatc gcgttaagcg aagaagaaag tagcgtggcg cacagttgcc
    31801 caatcaatta caccctcatt tcaaacggcc tggcaaacct gaccgataaa gtcgatcatg
    31861 tcgtggtaga agggactggc ggctggcgca gtctgatgaa tgatttgcgt ccactctctg
    31921 aatgggtagt gcaggaacaa ctgccggtgt tgatggttgt cggtattcag gaaggttgca
    31981 ttaaccatgc actgctaaca gctcaggcga tcgccaacga cgggctgccg ctcattggct
    32041 gggtggctaa ccgaatcaac ccaggactgg cgcattatgc ggaaatcatt gatgtgctgg
    32101 gtaaaaaact tccggcaccg ctcattggtg aactgcctta tctgccgcgc gccgaacagc
    32161 gtgaactggg gcaatacatc cgcttagcta tgctgcgcag tgtgctggcg gtagatagag
    32221 tcacggtgta acgtccgcga aattaccgac gcaattacgc aggcaatcaa taaaccgggg
    32281 agtagctgat actccccggt catttcacat atcatcaacg tcgacataat cggcgcgtgc
    32341 gtggttgccg ccaacagtgt cgccatcccg gtcaatccga gtaaaagcgt aatttcttcg
    32401 ccatcaggga accataatcc caggctacga ccatacaaca tgccaatggc aagaccgata
    32461 aatagcgtcg gtgtaaagac accaccgggt gcgccggaac cgctactcgc cagcacggca
    32521 cacagtttac aaaggaagat cccggcaatg atcattaaca gtggtggggc ggttaaaaag
    32581 gattgtacgg tgctatagcc gttgccccac actgcaggtg tgaacaggga aagcagaccc
    32641 acgatcaacc cgcctagtgc cagttgccag ggcggcgcaa gtttgagact cacaaatcca
    32701 cgatgacagg cgttcattaa cgttaacaac agtggtccgc acagacctgc cagcacacct
    32761 gtactgataa tcagcgcata gtcacgagcc tgaaccgtca ctgagagttg tacgttgtag
    32821 agtaacgcgt cgctatgatt aatcagattg ctaaccagcc atgccacgat ggcggaaata
    32881 atcaccgggc cgagagaggc caacatcata gtgccaaaca gcacttcggc tataaataaa
    32941 ctgccagcaa gcggggcacg ataggccgca gccattcccg ccgcggcccc acaggcgatc
    33001 cataatttcc attcctggcg tggcgtaaaa cgttgggcaa aacaggaggc ggcaagggca
    33061 gctaaaagaa tcatcgcacc ttcgcgacca attgcactgc cgctggttac taccagcagc
    33121 gaggcaagcg atttaaccag gcttgctgcg taatcgaact gtccatcggt ttgcaacgct
    33181 tccatgtaat cggtcggcgc atgagggcgt tgttgggtaa atttctgcca gcccatcagc
    33241 aacaaacccg ccgccagtcc gccgagcgcc ggagttagca gccgtcgcca ggaggaaagg
    33301 tttgtcgctg cattgaccag actgccggag tcattattga ggaacaacca ctccagtagc
    33361 agcatcgcat gacgaaaccc ggcaacggca aagacagcga gaataccgac gactgttgcg
    33421 ataagcagac ggcggaacat agtgcgcaga tccggataga tgtgtagatg atgcatgagc
    33481 agaaatgggc aatgaaacac gggcgactat tttgcggcaa aagaggccaa ccgcaaaagc
    33541 agtgcgtgct ttaagagata aaaaaacgtg acattgtaac gtattattgc cgaatcgtgc
    33601 gtaatcgtca ccatccggca atattacggt gatcctatcg aaagagtggt ttaaccgcga
    33661 caggaatcaa cagttgtgat tgccattgcg ccagcgttaa gcgagccagt tcacccagtg
    33721 cacggtagaa agggtgttct gctttttcga taaaaacatc aagaaaacgt gttgaccacg
    33781 gaaaaaggtg ccatgccagc aattcttcgc attctgtctg gcgaccattc tccgccaacc
    33841 acgcagccat caacagcagt gaaccaaaat gatcttccgg ttcgttttgc ttcatttcaa
    33901 actgaatgcc tttctcgcgc atccactgac gaagtgccaa tgttgaatcg ccaaacagca
    33961 cagattcgcg atccagccag accgaccccc acggcggaga cggcagtgcc cacgggccga
    34021 caaacaaacg ctgccaggcc tgggcgtgag tctcttcaca ctgtgtctga aaagccgtca
    34081 ccagcggcgc taatgacgct tctggtagag gccactgagt ttcccagccg tcactggtga
    34141 gtaccgcaac cagaggtgcg gcttccgcac tctctggagc gtaataaaac agcgcgccca
    34201 acacgcgcgc cgcgacagaa aaattatctt gctgtgaaaa atgggtcatt cctaacatcc
    34261 tgaaatgtgc gggttgcccc gcaccaattt gttaacctgc aatagccatt cctactgtca
    34321 tgtgcaggcc ataaaagagc acacgcccga tcatctcacc aacaagtatg aggattagcc
    34381 ccagaacaag tccggcaacg tgaggttcac ggcgacgaat gagcggacaa agccagcatc
    34441 caagacctgc gcataacaat accacacgcc agacctgtaa ggaggcgtaa tctggtacca
    34501 gtgcgctggc ttgttgcacg gatgagtgaa tagacgccag agacaaacct tgcagcacaa
    34561 tcacctcggc acaagcgatc agcgccagaa cgctaatgat ggcaaatggt gtggtattaa
    34621 atgtaacgcg ggctgcccgc agaattgcgg cagcaagtat tggaccgctc aacaataccg
    34681 tcaggaagaa agccagcgta gtgtaaccgt tatgccaggt tggcacggtg tcgatttgat
    34741 acacgcaggt catcatccag acgaaaatga cgccgagcgc catgctgaca agtaaccaga
    34801 gtttccccaa cgcttgcggc attttaccga tgaccgccac cagccaccat agtccgccaa
    34861 cggcaaaaaa tatggaaccg gcagcaatct cgttactcag accagaagct ccgattcgat
    34921 tcagcgagtt aaacgctcgc agtggcgatc ccaggtgcat gacagaggca ataaacccga
    34981 tgcccatcaa cagccagagg aaaaacatgc cacgaacaat gtgctgacgg tctgcgtcat
    35041 ttttggcggc gaaccagcca attccgctaa caatcagcgc gcctactacg cattgaccca
    35101 atacggtaaa gataaccagt ggccattcat gccatccatt tcccatttta cacttcctcc
    35161 ggattagcca gataaccagt ggtatcgccg gtcgggcggc tgttggcgtt aggtttgata
    35221 acgatattgg gttttgtgaa atgcgcgcgc ggcagtggcg cgacggcagc cagagtgccg
    35281 tgcttctggc gcagctcttc aattggaccg aactcaagtg cacgcagcgg gcaggattcg
    35341 acgcatatgg gttgtttccc ctcggcgacg cgcgaataac aaccatcgca cttcgtcatg
    35401 tgcccttttt cagcattgta ctgtggtgcg ccgtacggac aagccatgtg gcagtagcgg
    35461 cagccgatac aaacatcttc atcaaccacc acaaagccat cttcacgctt atgcattgcg
    35521 ccgctggggc agacttttgt acatgcgggg tcatcgcaat ggttacagga gatggagaga
    35581 taataagcga acacgttctg gtgccagatg ccattatctt cctgccagtc gccgccagca
    35641 tattcataaa tacggcggaa actgacttcc gggccaagat ctttaaaatc tttgcacgcc
    35701 agttcgcagg ttttacagcc agtacagcgg ctggaatcga taaaaaatcc atattgtgtg
    35761 gtcatgggct actccttaaa ccttttcgat ctggacaaga ttgctgtgcg acgggtttcc
    35821 ctttgccagc ggtgaagggc ggtgagaggt cagaatattg atactgccgc cgtgatcgac
    35881 ccggtcacca aacatatccg ctttaagcca cgcaccttgc ccgatggcgg taacgccagg
    35941 cagaatacgc ggagtcactt ttgcggcaat cagcatttct ccattattgt taaatacccg
    36001 cacggtatcg ccatgacgga taccgcgtgc ctgagcatca atggggttga tccacacctc
    36061 ttgtgggcag gcctgctgta acacatcaat attgccgtag ctggagtggg tacgcgcttt
    36121 ggtttggcga cttcactcag catccagtag atgggtttgc gttcaaattt tgctgaggtt
    36181 gcgggttggg cgaggatcac atagcccata ttccctgcag attcatgaga gataaggtct
    36241 tcttgctctg ttggcatcag gtcaggcaac aggatatcgc aatacttagc cgaggccgtc
    36301 atgaagtggt caatgccaac aatcatctcg cacttgctgt catcctgaag cacctcatgg
    36361 gtgtgattga tgtcgccatg ttgattgatc aatgtgttac tggcgtagca ccataaaaac
    36421 ttgatgggga catccagttt ttcttttcca cgaacaccat cacgggtcgc ggtcattttc
    36481 gtaccatggt cgatggcatc tgtccatgta aaaacggaaa tctgcgtttt aacaggattc
    36541 tcgagcatcg ggaaccattc tacccccaga tcccagctac cttcgcgtac acctgagttg
    36601 ccgccgttta tgccgacgtt accggtaaga acggaaagca tggcaatagc gcgggacgtt
    36661 tgttcgccgt tggagtgtcg ttgtggcccc caaccctgac aaatataagc aggttttgct
    36721 gaaccgatct ctcgtgccaa ctggataatt ttttctgccg ggatgctggt gatttttgct
    36781 gcccattccg gcgttttagc tatgccgtca ggcccttcgc ccagaatata ggctttataa
    36841 tgcgcgttac gtggtgcgtt ggcgggcagc gttttttcat cgtaaccaac acaatatttg
    36901 tcgagaaatg gctgatcgac catgttttca gtaatcagta cccaggcaat cgcacaggcc
    36961 agtgcgccat cggtgccagg gcgaatgggc agccattcat cttcacgccc ggcagctgtg
    37021 tcgttatagc gtggatcgat gacgatcatg cgtgcgtttg aacgttcgcg ggcttgctcg
    37081 acgtagtaag tgacaccacc gccgctcatc cgcgtttctg ccgggttatt tccgaacata
    37141 acgaccagtt tcgtattggc gatatcatcc gggctgttgc catcattggc accgaacata
    37201 taactcattg cggcactgat ctgtgcggta ctgtagctgc catagcgact gagaaaaccc
    37261 ccgcaagagt tcatcagacg gtacgggacg ttcgagttgg tgatatttcc gccatctacg
    37321 cctgttccgt acaggacatg tacagcctca ttgccgtaat ctttcaggat ccgccgaaga
    37381 ttatcactga tggtatccag ggcttcgtcc caacttatcc gttcaaattt accttcaccg
    37441 cgctcgccga cgcgcttcat gggatatttc aacctgtcag gatgattcat ccgtcggcgg
    37501 atagagcgcc cgcgtaaaca tgcccgaacc tgatgattac cgtaaacgtc gtcacctgtc
    37561 gtatcagact ccacccagta cacggtgtca tctttcacat gcaaacgtag cagacagcgg
    37621 ctcccgcagt taacggtgca ggaactccag accgttttct cttctaccgg agcctctgcc
    37681 gcccggacca tttgggaaaa tggcagagtg aaagcactgc ttgccagcgc gagactgcca
    37741 agtgcggagg ttttcatcag acttctatgg ctgatttcag ccttcatgag cgcctctgtg
    37801 gtatggattt tcatcattac tcacttattg cttttcaaac aaaatgtcat gccagaattt
    37861 atggttgtag tgggttatat tttttcgatc tcgaccagat tagtgtgctg cgggtttccc
    37921 ttcgccagtg gtgaagggcg cagagtggtt agcgtattca cacatccgcc atggtcgatt
    37981 ttatcgccag acatattggg ctcgtgccag gctccctggc ccatagcgct aactccaggg
    38041 agaatgcgtg gtgttacttt ggctggtagc cgaacttcgc cacgatggtt aaacacccgc
    38101 accatatcgc cgttggcaat cccacgtttc tgtgcatcta tagggttgat ccacacctcc
    38161 tgacggcagg ctgccttcag gacatcaatg ttgccgtagg tcgagtgagt acgggatttg
    38221 taatggaaac caaacagttg cagtgggaag gttctacgtt caggggagtc ccagccttca
    38281 aaggttgagg cataaactgg cagtgggctt atcacttcat ctttttccag ttcccaggta
    38341 cgggcaattt ccgccagctt gctggaataa atttcaatct taccggaagg cgttttaagt
    38401 ggatttgcct cggggtcgtc acgaaatgct ttgtaggcga caaaatggcc attgggatct
    38461 ttacgcttat agatacccat ttttttcagt tcgtcgtaag acggtaacgc cggatctttg
    38521 gcaagcattt tggcgtacag atgttgtaac cattgttcct gcgtgcgacc ttctgtgaac
    38581 ttttgataga cgtcaggtcc aagacgtttc gcgacttcac tcaggatcca gtaaatcggt
    38641 ttacgttcga atttttcgct ggtgactggc tggaggaaaa tgagatatcc catgttaccg
    38701 gcgtagtcgt taggaataat atcttcctgc tcaacggtca tcaggtcttc cagcagaatg
    38761 tcggcatatt ttgccgatga ggtcataaag ttttcgatga ccacaatcat ttcgcatttc
    38821 gattcgtcct gcagaatttc atgcgttttg ttgatgtcag aatgctgatt aacgagggta
    38881 tttcccgcgt agttccagat ggactccatt gccagacgga tggatggact tgaagtagtt
    38941 gactcaatgc tacttaaggt cctgatatga ctgacattaa tcatgataac actgtatatc
    39001 catccagttc tttctctggg tctccctatc ggtatcagca agtctacacc tcaagccctg
    39061 acacaaggct gacacaaatc aatctactca gcgtagctaa gcctcagtta gtccgcagag
    39121 ccaatggtcg atatactatc cgcttcagac tcaagggaca gaccacaccg tttctttcag
    39181 tatcgacccg cagcacagac aggagagtag ccacaatgcg tcagagagag cttgcagcca
    39241 ccgctaaggc cttcatgttg gataggcctg aagtctcact acaggagctt acagagcatc
    39301 tccggtctat ggctgagcag ttcctgacag acgctagtga cgattactgg aatggacttg
    39361 aggtcgctac tctggtcgac gagaagtcca acctgaagga gctagccgct acgcaagcct
    39421 tgagtctgga ccagcagaag ggcataaggt tagcgctgga agtcctgacc gcagcacagc
    39481 agagagtcga cactggtgat acctctggtc tgattaagct gattgatgat aataacctta
    39541 ctgatgactc aactataggc gactctacgt caattctgaa taatgagcag ggggatagac
    39601 cggcagtttt cacacaggag cgccagtcat ctgtagtttt ttcaagccta gtgtcctcgc
    39661 tgctggctga gaaggtccag accctgaaga catccagcta caaggacctc tcgtcctcac
    39721 tcaatactgt ctcccggttc ctgcctgaag acatggacct gatgtcccgc tctgggtggc
    39781 tggctgtcag ggactctatg ctggcttctg aggtgagacc atcgacaatc aacaagctgc
    39841 tgaccaaagc gaagatgtgt ctcgattatg gcctgatgaa tgggcaactt gagggccgca
    39901 accctattga acggatgaag ctgaccaagg atatcgactc caagcgcaga gcgttcacag
    39961 acgaggagct ggagagactt ctggtccgtg ttgagtctg
//
LOCUS       DAJTUI010000028        38198 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTUI010000028 DAJTUI010000000
VERSION     DAJTUI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.028"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            93..134
                     /gene="hisL"
                     /locus_tag="OO464_001589"
     CDS             93..134
                     /gene="hisL"
                     /locus_tag="OO464_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR6226778.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    110..233
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            280..1179
                     /gene="hisG"
                     /locus_tag="OO464_001590"
     CDS             280..1179
                     /gene="hisG"
                     /locus_tag="OO464_001590"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6226779.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1185..2489
                     /gene="hisD"
                     /locus_tag="OO464_001591"
     CDS             1185..2489
                     /gene="hisD"
                     /locus_tag="OO464_001591"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6226780.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2486..3556
                     /gene="hisC"
                     /locus_tag="OO464_001592"
     CDS             2486..3556
                     /gene="hisC"
                     /locus_tag="OO464_001592"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6226781.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3556..4623
                     /gene="hisB"
                     /locus_tag="OO464_001593"
     CDS             3556..4623
                     /gene="hisB"
                     /locus_tag="OO464_001593"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6226782.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4623..5213
                     /gene="hisH"
                     /locus_tag="OO464_001594"
     CDS             4623..5213
                     /gene="hisH"
                     /locus_tag="OO464_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6226783.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5213..5950
                     /gene="hisA"
                     /locus_tag="OO464_001595"
     CDS             5213..5950
                     /gene="hisA"
                     /locus_tag="OO464_001595"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6226784.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            5932..6708
                     /gene="hisF"
                     /locus_tag="OO464_001596"
     CDS             5932..6708
                     /gene="hisF"
                     /locus_tag="OO464_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6226785.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            6702..7313
                     /locus_tag="OO464_001597"
     CDS             6702..7313
                     /locus_tag="OO464_001597"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6226786.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7499..8476)
                     /gene="wzzB"
                     /locus_tag="OO464_001598"
     CDS             complement(7499..8476)
                     /gene="wzzB"
                     /locus_tag="OO464_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6226787.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(8622..9742)
                     /gene="ugd"
                     /locus_tag="OO464_001599"
                     /pseudo
     CDS             complement(8622..9742)
                     /gene="ugd"
                     /locus_tag="OO464_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(9992..11398)
                     /gene="gndA"
                     /locus_tag="OO464_001600"
     CDS             complement(9992..11398)
                     /gene="gndA"
                     /locus_tag="OO464_001600"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6226788.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11507..11866)
                     /locus_tag="OO464_001601"
     CDS             complement(11507..11866)
                     /locus_tag="OO464_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226789.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(11863..12369)
                     /locus_tag="OO464_001602"
     CDS             complement(11863..12369)
                     /locus_tag="OO464_001602"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226790.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12515..13078)
                     /locus_tag="OO464_001603"
     CDS             complement(12515..13078)
                     /locus_tag="OO464_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226791.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13285..14223)
                     /locus_tag="OO464_001604"
                     /pseudo
     CDS             complement(13285..14223)
                     /locus_tag="OO464_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14286..14660)
                     /locus_tag="OO464_001605"
     CDS             complement(14286..14660)
                     /locus_tag="OO464_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR6226792.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(14664..15812)
                     /gene="wzy"
                     /locus_tag="OO464_001606"
     CDS             complement(14664..15812)
                     /gene="wzy"
                     /locus_tag="OO464_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR6226793.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(15832..16737)
                     /gene="rfbG"
                     /locus_tag="OO464_001607"
     CDS             complement(15832..16737)
                     /gene="rfbG"
                     /locus_tag="OO464_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR6226794.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(16730..17620)
                     /locus_tag="OO464_001608"
     CDS             complement(16730..17620)
                     /locus_tag="OO464_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR6226795.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(17650..18906)
                     /gene="wzx"
                     /locus_tag="OO464_001609"
     CDS             complement(17650..18906)
                     /gene="wzx"
                     /locus_tag="OO464_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR6226796.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(18922..19467)
                     /gene="rfbC"
                     /locus_tag="OO464_001610"
     CDS             complement(18922..19467)
                     /gene="rfbC"
                     /locus_tag="OO464_001610"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR6226797.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19472..20350)
                     /gene="rfbA"
                     /locus_tag="OO464_001611"
     CDS             complement(19472..20350)
                     /gene="rfbA"
                     /locus_tag="OO464_001611"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6226798.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20409..21308)
                     /gene="rfbD"
                     /locus_tag="OO464_001612"
     CDS             complement(20409..21308)
                     /gene="rfbD"
                     /locus_tag="OO464_001612"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR6226799.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21308..22393)
                     /gene="rfbB"
                     /locus_tag="OO464_001613"
     CDS             complement(21308..22393)
                     /gene="rfbB"
                     /locus_tag="OO464_001613"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6226800.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(22766..23659)
                     /gene="galF"
                     /locus_tag="OO464_001614"
     CDS             complement(22766..23659)
                     /gene="galF"
                     /locus_tag="OO464_001614"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR6226801.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(23834..25228)
                     /gene="wcaM"
                     /locus_tag="OO464_001615"
     CDS             complement(23834..25228)
                     /gene="wcaM"
                     /locus_tag="OO464_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR6226802.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25239..26459)
                     /gene="wcaL"
                     /locus_tag="OO464_001616"
     CDS             complement(25239..26459)
                     /gene="wcaL"
                     /locus_tag="OO464_001616"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR6226803.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26456..27736)
                     /gene="wcaK"
                     /locus_tag="OO464_001617"
     CDS             complement(26456..27736)
                     /gene="wcaK"
                     /locus_tag="OO464_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR6226804.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(27916..29394)
                     /gene="wzxC"
                     /locus_tag="OO464_001618"
     CDS             complement(27916..29394)
                     /gene="wzxC"
                     /locus_tag="OO464_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR6226805.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29396..30790)
                     /gene="wcaJ"
                     /locus_tag="OO464_001619"
     CDS             complement(29396..30790)
                     /gene="wcaJ"
                     /locus_tag="OO464_001619"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR6226806.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(30845..32215)
                     /gene="cpsG"
                     /locus_tag="OO464_001620"
                     /gene_synonym="manB"
     CDS             complement(30845..32215)
                     /gene="cpsG"
                     /locus_tag="OO464_001620"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6226807.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32408..33844)
                     /gene="cpsB"
                     /locus_tag="OO464_001621"
     CDS             complement(32408..33844)
                     /gene="cpsB"
                     /locus_tag="OO464_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6226808.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(33847..35070)
                     /gene="wcaI"
                     /locus_tag="OO464_001622"
     CDS             complement(33847..35070)
                     /gene="wcaI"
                     /locus_tag="OO464_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR6226809.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35067..35546)
                     /gene="gmm"
                     /locus_tag="OO464_001623"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35067..35546)
                     /gene="gmm"
                     /locus_tag="OO464_001623"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6226810.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35546..36514)
                     /gene="fcl"
                     /locus_tag="OO464_001624"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35546..36514)
                     /gene="fcl"
                     /locus_tag="OO464_001624"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6226811.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36517..37638)
                     /gene="gmd"
                     /locus_tag="OO464_001625"
     CDS             complement(36517..37638)
                     /gene="gmd"
                     /locus_tag="OO464_001625"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6226812.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(37664..>38198)
                     /gene="wcaF"
                     /locus_tag="OO464_001626"
     CDS             complement(37664..>38198)
                     /gene="wcaF"
                     /locus_tag="OO464_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6226813.1"
                     /translation="GFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRAFLLRL
                     FGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQKSYLC
                     TGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKSLPANV
                     VCRGNPAVVIRERVETE"
BASE COUNT        10211 a         9580 c         8612 g         9795 t
ORIGIN      
        1 gccataaaat atataaaaaa gcccttgctt tctaacgtga aagtggttta ggttaaaaga
       61 catcagttga ataaacattc acagagattt ttatgacacg cgttcaattt aaacaccacc
      121 atcatcctga ctagtctttc aggcgatgtg tgctggaaga cattcagatc ttccagtggt
      181 gcatgaacgc atgagaaagc ccccggaaga tcaccttccg ggggcttttt tattgcgcgg
      241 ttgttaacgg ttcagacagg tttaaagagg aataagaaaa tgacagacaa cactcgttta
      301 cgcatagcta tgcagaaatc cggccgttta agtgatgact cacgcgaatt actggcgcgc
      361 tgtggcatta aaatcaatct tcacacccag cgcctgatcg cgatggcaga aaacatgccg
      421 attgatattc tgcgcgtgcg tgacgatgac attccctgtc tggtaatgga cggcgtggta
      481 gaccttggga ttatcggcga aaacgtgctg gaagaagagc tgcttaaccg ccgcgcccag
      541 ggtgaagatc cgcgctactt taccctgcgt cgtctggatt tcggcggctg ccgcctttcg
      601 ctggcaacgc cggttgatga agcctgggac ggtccgctct ccttaaacgg taaacgtatc
      661 gccacctctt atcctcacct gctcaagcgt tatctcgacc agaaaggtat ctcttttaaa
      721 tcctgcttac tgaacggttc tgtcgaagtt gcgccgcgtg ccgggctggc ggatgcgatt
      781 tgcgatctgg tttccaccgg tgccacgctg gaagctaacg gcctgcgcga agtcgaagtt
      841 atctaccgct cgaaagcctg cctgatccag cgcgatggcg aaatggaaga atctaaacag
      901 caactgatcg acaagctact gacccgtatt cagggtgtga tccaggcgcg cgaatcaaaa
      961 tacatcatga tgcacgcacc gaccgaacgc ctggatgaag tcatcgccct gctgccaggt
     1021 gccgaacgcc caaccattct gccgctggcg ggtgatcaac agcgcgtagc gatgcacatg
     1081 gtgagtagcg aaaccctgtt ctgggaaacg atggaaaaac tgaaagcact gggtgccagt
     1141 tcaatcctgg tcctgccgat tgagaagatg atggagtgat cgccatgagc tttaacacaa
     1201 tcattgactg gaatagctgt actgcggagc aacaacgcca gctgttaatg cgcccggcga
     1261 tctccgcttc tgaaagcatt acccgcaccg ttaacgatat tctcgataac gtgaaaacgc
     1321 gtggcgatga ggccctgcgt gaatacagcg cgaagtttga taaaaccacg gttaccgcgc
     1381 tgaaggtgtc tgctgatgag atcgccgccg ccagcgaacg cctgagcgac gagctaaaac
     1441 aggcgatggc ggtggcagta aagagtattg aaaccttcca cactgcgcaa aaactgccgc
     1501 cggtagatgt agaaacgcag ctaggcgtgc gttgccagca ggtcacgcgc ccggtggctt
     1561 cagtgggttt gtatattccg ggcggctccg ccccgctctt ctcaacggta ttaatgctgg
     1621 caactccggc gcgtattgcg ggctgtaaaa aagtggtgct gtgctcaccg ccgccgattg
     1681 ccgatgaaat tctttatgcg gcgcagctgt gcgatgtgca ggacgtgttt aacgtcggcg
     1741 gcgcacaggc cattgccgcg ctggcgtttg gtacggaatc tgtgccgaaa gtggacaaaa
     1801 tcttcgggcc gggtaacgcc tttgtcaccg aagcaaaacg ccaggtaagc cagcgtctgg
     1861 acggtgcggc gatcgatatg cccgcaggcc cgtcggaagt gctggtgatt gctgacagcg
     1921 gcgctacgcc ggattttgtg gcttctgatt tgctttctca ggctgaacac ggccctgact
     1981 cacaggtaat tttactgacc cccgatgctg atatggcgcg tcgcgttgcc gaggccgtcg
     2041 aacgccaact ggcagaactg ccgcgtgccg aaaccacccg ccaggcactg aacgccagcc
     2101 gcctgatcgt gactaaagat ttagcgcagt gcgtggagat ctccaaccag tacggcccgg
     2161 agcacctgat cattcagacc cgcaacgccc gcgaactggt cgatggcatc accagcgccg
     2221 gttcggtatt tcttggtgac tggtcaccgg aatcggcagg cgactacgcc tccggcacca
     2281 accacgtgct gccgacttac ggttacaccg ccacctgttc cagcctcggg ctggcagatt
     2341 tccagaaacg catgaccgta caggaactgt cgaaagaagg tttctccgcg ctggcttcga
     2401 ccattgaaac actggccgcc gccgagcgcc tgaccgccca caaaaatgcc gttactttgc
     2461 gtgttaacgt ccttaaggag caagcatgag caccgtgact attactgatt tagcgcgtga
     2521 aaacgtccgc aacctgacac cgtatcagtc ggcgcgtcgt ctgggcggta acggcgacgt
     2581 ctggctgaac gccaacgaat atcccacagc cgtggagttt aagcttactc agcaaacgct
     2641 caaccgctac ccggaatgcc agccaaaagc ggtgattgaa aattacgcgc aatatgcagg
     2701 cgtaaaaccg gaacaggtgc tggtcagccg tggcgcggac gaaggtattg aactgctgat
     2761 ccgcgctttt tgcgaaccgg gtaaagacgc catcctctac tgcccgccaa cgtacggcat
     2821 gtacagcgtc agcgctgaaa ccattggtgt cgaatgccgc acagtgccga cgctggaaaa
     2881 ctggcaactg gacttacagg gcatttccga caagctggac ggcgtaaaag tggtctatgt
     2941 ttgcagcccc aacaacccca ccgggcaact gatcaatccg caggattttc gcaccctgct
     3001 ggagctaacc cgcggtaagg cgattgtggt tgccgatgaa gcttatatcg agttttgccc
     3061 gcaggcatcg ttagctggct ggctggcgga atatccgcac ctggcgattt tgcgtaccct
     3121 ttcgaaagcc ttcgcgttgg cggggcttcg ttgcggattt acgctggcaa acgaagaagt
     3181 catcaacctg ctgatgaaag tgatcccccc ctacccgctc tcgacgccgg ttgccgacat
     3241 tgccgcacag gcgttaagcc cgcagggaat cgtcgccatg agcgaacggg tggcgcaaat
     3301 tattacagaa cgcgaatacc tgattgccgc actgaaagag atcccctgcg tggagcaggt
     3361 tttcgactct gaaaccaact acattctggc gcgctttaaa gcctccagtg cagtgtttaa
     3421 atctttgtgg gatcagggca ttatcttacg tgatcagaat aaacaaccct ctttaagcgg
     3481 ctgcctgcga attaccgtcg gaacccgtga agaaagccag cgcgtcattg acgccttacg
     3541 tgcggagcaa gtttgatgag tcagaagtat ctttttatcg atcgcgatgg aaccctgatt
     3601 agcgaaccgc cgagtgattt tcaggtggac cgttttgaca aactcgcctt tgaaccgggc
     3661 gtgatcccgg aattgctgaa gctgcaaaaa gcgggctaca agctggtgat gatcactaat
     3721 caggatggtc tgggaacaca aagtttcccg caagcggatt ttgatggccc gcacaacctg
     3781 atgatgcaga tcttcacctc gcaaggcgtg cagtttgatg aagtgctgat ttgtccgcac
     3841 ctgcccgccg atgagtgcga ctgccgtaag ccgaaagtaa aactggtgga gggttatctg
     3901 gctgagcaag cgatggatcg tgccaacagt tatgtaattg gcgatcgcgc gaccgacatt
     3961 caactggcgg aaaacatggg cattaatggt ttacgctacg accgcgaaac cctgaactgg
     4021 ccaatgattg gcgagcaact cactaaacga gaccgttacg cccatgtagt gcgcaacacc
     4081 aaagagacgc agattgacgt ccaggtgtgg ctggatcgcg aaggtggcag caagattaac
     4141 accggcgttg gcttctttga tcacatgctg gatcagatcg ccacccacgg cggtttccgc
     4201 atggaaatca atgtcaaagg cgacctctat atcgacgatc accacaccgt cgaagatacc
     4261 ggcctggcgc tgggcgaagc gttaaaaatt gccctcggcg acaaacgcgg tatttgccgc
     4321 tttggttttg tgctgccgat ggacgaatgc cttgcccgct gcgcactgga tatctctggt
     4381 cgcccgcacc tggaatataa agccgagttt acctaccagc gtgtgggcga tctcagcact
     4441 gaaatgatcg aacacttctt ccgttcgctc tcttacacca tgggcgtgac actacacctg
     4501 aaaaccaaag gtaaaaacga tcatcaccgt gtagaaagcc tgttcaaagc ctttggtcgt
     4561 accctgcgcc aggccatccg cgtcgaaggc gacaccctgc cctcgtcgaa aggagtgctg
     4621 taatgaacgt ggtgatcctt gatactggct gcgccaacct gaactctgtg aagtctgcca
     4681 ttgcgcgcca cggttatgaa cccaaagtca gccgtgaccc ggacattgtg ttgctggccg
     4741 ataaactgtt tttacccggc gtcggcaccg cacaagcagc gatggatcag gtgcgtgagc
     4801 gcgagctgtt tgatctcatc aaagcctgta cccaaccggt gctgggcatc tgcttaggga
     4861 tgcaactcct gggacgacgc agcgaagaga gcaatggcgt cgacttgctg ggcatcatcg
     4921 acgaagacgt gccgaaaatg accgactttg gtctgccact gccacatatg ggctggaacc
     4981 gcgtttatcc gcaggcaggc aaccgcctgt ttcaggggat tgaagacggc gcgtactttt
     5041 actttgttca cagctacgca atgccagtca atccgtggac catcgcccag tgtaattacg
     5101 gcgaaccgtt caccgcggcg gtacaaaaag ataacttcta cggcgtgcag ttccacccgg
     5161 agcgttctgg tgccgctggc gctaagttgc tgaaaaactt cctggagatg tgatgattat
     5221 tccggcatta gatttaatcg acggcaccgt ggtgcgtctc catcagggcg attacggcaa
     5281 acaacgcgat tacggtaacg acccgctgcc gcgcttacag gattacgctg cgcaaggtgc
     5341 cgaagtgttg cacctggtgg atctgaccgg ggcaaaagat ccggctaagc gtcaaatccc
     5401 gctgattaaa accctggtcg cgggcgttaa cgtcccggtg caggttggcg gcggtgttcg
     5461 tagtgaagaa gacgtggcgg cgttactgga agctggcgtt gcgcgcgtgg tggtcggttc
     5521 caccgcagtg aaatcaccgg acgtagtgaa aggctggttt gaacgtttcg gtgccgatgc
     5581 gctggtgctg gcactggatg tccgtattga cgagcaaggc aacaagcaag tggcagtcag
     5641 cggctggcaa gagaactcgg gcgtttcact ggaacaactg gtggaaacct atctgcccgt
     5701 cggcctgaaa catgtgttgt gtaccgatat ctcgcgcgac ggcacgctgg cgggatctaa
     5761 cgtctcttta tatgaagaag tgtgtgccag atatccgcaa gtggcgtttc agtcctccgg
     5821 cggcattggc gacattaacg atgttgctgc cctacgtggc actggcgtgc gcggcgtaat
     5881 agttggtcgt gcattactgg aaggtaaatt caccgtgaag gaggccatcg catgctggca
     5941 aaacgcataa tcccgtgtct cgacgttcgt gatggtcagg tggtgaaagg cgtacagttt
     6001 cgcaaccatg aaatcattgg cgatatcgtg ccactggcaa aacgctacgc cgaagaaggt
     6061 gcagatgaac tggtgttcta cgatatcacc gcttccagcg atggccgtgt ggtagataaa
     6121 agctgggtat ctcgcgtggc ggaagtgatc gacattccgt tttgtgtggc gggtgggatt
     6181 aagtctctgg aagatgccgc gaaaattctt tcctttggcg cggataaaat ttccatcaac
     6241 tcccctgcgc tggcagaccc aacattaatt actcgcctgg ccgatcgctt tggcgtgcag
     6301 tgtattgtgg ttggtattga tacctggtac gacgccgaaa ccggtaaata tcatgtaaat
     6361 caatataccg gcgatgaaag ccgcacccgc gtcactcaat gggaaacgct cgactgggta
     6421 gaggaggtgc aaaaacgcgg cgccggagaa atcgtcctca acatgatgaa tcaggacggc
     6481 gtgtgtaacg gttacgacct caagcagctg aaaaaagtgc gtgaagtttg ccacgtcccg
     6541 ctgattgcct ccggtggcgc gggcaccatg gaacacttcc tggaagcctt ccgcgatgcc
     6601 gacgttgacg gtgcgctggc agcttccgta ttccacaaac aaataatcaa tattggcgaa
     6661 ttaaaagcgt acctggcaac acagggcgtg gagatcagga tatgttaaca gaacaacaac
     6721 gtcgcgaact ggactgggaa aaaaccgacg gtctgatgcc ggtgattgtg caacacgcgg
     6781 tatctggcga agtgttaatg ctgggctaca tgaacccgga agccttagac aaaaccatcg
     6841 aaagcggtaa agtcaccttc ttctcgcgca ctaaacagcg actgtggatc aaaggcgaaa
     6901 cctcgggcaa ttttctcaat gtggtaagta ttgccccgga ctgcgacaac gacacattac
     6961 tggtgctggc gaatcccatc gggccaacct gccacaaagg caccagtagc tgcttcggca
     7021 acaccgctca ccagtggctg ttcctgtatc aactggaaca actgctcgcc gaacgtaaat
     7081 atgccgaccc ggaaacctcc tacaccgcca aactgtatgc cagcggcact aaacgcattg
     7141 cgcagaaagt gggcgaagaa ggcgtggaaa ccgcactggc agcaacggta catgatcgct
     7201 ttgagctgac caacgaggca tctgacttga tgtatcacct gctggtgctg ttgcaggatc
     7261 aggatctgga tttaacgaca gtaattgaga acctgcataa acggcatcag tgagttacgg
     7321 gctgagcagg tgtgatgttg ttgtcacacc cggagccatt gcccgatgcg caaacttatc
     7381 gtgcctacac attatctcaa cacacgtagg ccggataaga tgcgccagca tcgcatccgg
     7441 caaaaaaaac gggcatggtg tcaccaccct gcccgtttct cttaaatgca caataatatt
     7501 acttcgcgtt gtaattacgc agagcgttac gccccaacac aatccccgcg ccaaccatgc
     7561 cgcccagaag cactgccaga atcaaggtga ttgccttttt cggactatcg cgacgaatag
     7621 gtaacgtcgg tttcatcaca tagcggtaag catgaatatc aagatcatca aactttaatt
     7681 tttcaatatc caacaggttt tgacgtgtct gatagtagtt tggtgagaac accaacggac
     7741 gagtcgcttc atgcttaatc atcgactcca gcgcttcgct ccctagaagg aacaacgtat
     7801 cttgcgtcac atcttcagtc tgctgaacct gtggctttgt cacctgcgcc tgattcgcat
     7861 actgcaacgc ttcctgaatc tgacggatac gcagatcttt ctgctcctgc gcgaccactt
     7921 cctgggttct taaagagtcc tgcaagtttt tccgtcccag agcaatgttg tccttgagat
     7981 ccttttctag ctcttgattc actttatcat caacttgctg aatgtattgg gccaacttca
     8041 tttgtgcgcc ctctgcagtt tgcccaacat aagaaacagt caatggtaat tgctggttct
     8101 taacagaagg ttcgatggta agtttttctg gctcttcctg attatccagc gtttctgcta
     8161 atgcagagaa ggcagaactg aagcgaccaa ttaacgtctc ctgcaaatcc gatactttcg
     8221 gtgcagcctg accatagata acattcatgg cattgttata gccagcaatt tgccccacgt
     8281 cgggctgagt gataattgct gttgacgtcc atttctcctt cgctactgcc aaataaccaa
     8341 tagccagggc aatagccaca atgacggaaa tgataattgt catcttgcca cgccacaact
     8401 gcactagtaa atcaatcaaa tcaatctgtt ccgggtcatg gttttgccca gaaacattat
     8461 tattttctac tctcatcatt accctaactg acggaaattt ccaagtccgc atagtttagc
     8521 gataatcccc gtgaatgcta taggataagt gataaaaata atgaattatt gcggaggtaa
     8581 caaaagacaa tcagggcgta aatagccctg ataacaagat gttagtcgct gccaaagaga
     8641 tcgcgggtgt agaccttatc tgccacatcc ttaagctctt ctgccatacg gttggagata
     8701 atgacgtcgg cttgttgttt gaaggtggcg agatcacgtt ccaggcgaga gttgaagaat
     8761 gagtcttctt tcatcaccgg ctcgtagatg atcacttcaa cgcctttcgc cttgatacgc
     8821 ttcataatcc cctgaataga agacgcacgg aagttatctg acccgctctt cataatcaga
     8881 cgataaatac ccaccacttg cggtttgcat cgacaattgc cgagatcagg ttattcggca
     8941 cagactggta gttcgccagt aactgcttgg tatctttcgg cagacaataa ccaccataac
     9001 caaacgacgg attgttgtaa tggttgccaa tacgcggatc gagacaaacg ccttcgataa
     9061 tctggcgagt attcagacct aaactttctg cataactatc cagttcatta aagtatgcta
     9121 cgcgcatcgc cagataggta ttagcgaaaa gtttaatcgc ttctgcttca gtggagtcgg
     9181 taaacagggt cgggatattt tgcttaatcg cgccttcctg taacaacgca gcaaaacgtt
     9241 ctgcgcgttc tgaacgctca ccgatgacaa tacgtgacgg atggagatta tcgtaaaggg
     9301 ctttaccttc gcggaggaat tccggagaga aaataatatt ttcagtacga tatttcttat
     9361 gcatcgctgc ggtaaaacca acgggaaccg ttgatttgat gaccataacc gcataaggat
     9421 ttatctcaac tacgtcttta attactgatt ctacactgga tgtattgaaa taattagttt
     9481 taggatcata gtcggttgga gtggcgatga tgacataatc agcatcccgg taggcttcat
     9541 ttttatctaa tgtggcatta aagtgtattt tatctgattg caaaaactgc tgaatttcct
     9601 tatcaacaat aggagatatc cgatcattca gcatagcaac gcgtgacggt aaaatatcta
     9661 atgccacaac ctcatgattt tgtgcgatta gaagcccgtt tgacaagcct acatagccag
     9721 taccggaaat ggtgattttc atttcatgct ctcagaatta acttaactgt gaatcatgat
     9781 gtttttagca tcgtgataag atgctaaaag ttttaacgct acgttgtcag atggtcaacg
     9841 caacgaaaaa caattcagat tttctttata agaatagctg atgtttatta agttaatatt
     9901 aagcagtgaa atttagtcta taagatattt ggcaaaaaaa agcccggtgc aatatacgcc
     9961 gggcctcaat tttattgttg gttaaatcag attaatccag ccattcagta tggaacacac
    10021 cttctttatc aatgcgctta taagtatgcg ccccgaaata gtcacgctgt gcctggatca
    10081 ggttcgcagg cagaaccgcg gcacggtagc tatcgtaata ggcaaccgca gcggcgaagg
    10141 tcggaaccgg gataccgttc tgtactgcat aagcgacgac atcgcgcagc gcctgctggt
    10201 agtcatcggc aatttgcttg aagtacgggg ccagcagcag gttagcgatc tgcggatttt
    10261 cggcataagc atcggtgatt ttctgcagga actgcgcacg gatgatgcag ccagcacgga
    10321 aaatcttcgc gatttcgccg tagttcagat cccaattgta ttcttcagac gcagcacgca
    10381 gctgagagaa gccctgagcg taagaaacga ttttgcccag atacagcgca ctgcgaactt
    10441 tttcgatgaa ctcagccttg tcgcctgctg actgagcttg cggaccagag agaactttag
    10501 atgcggcaac gcgctgatct ttcagagaag agatataacg tgcaaacaca gactcggtaa
    10561 tcagcgacag cggttcgccg agatccagcg cgctctggct ggtccattta ccggtgcctt
    10621 tgtttgctgc ttcatccaga atcacatcaa ccaggtagtt accgtcttca tcttttttgg
    10681 tgaagatatc tttagtgatg tcgatcaggt agctgctaag ttcaccgtta ttccactcgg
    10741 tgaaagtctg ggccagttct tcattagata gattcaggcc acctttgagc agagaatacg
    10801 cttcagcaat gagctgcata tctccgtatt caataccatt gtgaaccatc ttcacataat
    10861 ggcctgcgcc gtcggcacca atataagtaa cgcatggctc gccgtcttct gccacggcag
    10921 cgattttagt caggataggc gcaaccagct cataagcctc tttctgcccg ccaggcataa
    10981 tagaaggacc tttcagcgcg ccctcttcac cgccggaaac accggtaccg atgaagttaa
    11041 agccctctgc tgaaagctca cgattacgac gaatggtgtc ctggaagaag gtattaccac
    11101 catcaatgat gatgtcacct ttatcgagat atggtttcag ggaatcaatc gcagcatccg
    11161 tgcctgcacc tgctttcacc attaacagga tgcgacgagg cgtttccaga gattcaacaa
    11221 actctttcac cgtatagtaa ggagccagtt tcttgcctgg attttcagca atcacttctt
    11281 ccgtcttttc gcgggagcgg ttgaaaatag agacggtata accacgactt tcgatgttga
    11341 gcgcaaggtt gcgccccatc actgccatac cgactacgcc gatctgttgc tttgacattg
    11401 tttactcctg tcttctatat tattaaccgt ctattaaccg ttatattatt aaccgtcgtg
    11461 attttcatct tctaatcaag gaatacatat ggttacagca tgcttcttac tttatagcga
    11521 taagtatgcc attgggtgta tccataagaa ctttgaattt tggatacttt tctggattaa
    11581 aattagcata gcttcgttta gatataacaa taaacgaact attaaaagat ttcatcttct
    11641 ctattaaatc atcctgtggg attacctcac actcgggatt actaatagtc caatcactat
    11701 atcttgctaa tccaaagacg tcaccaattg ccatcccttt atagaagtaa ataccatcct
    11761 ccaagcctaa ttggataaga ttatctccat ataactcact atattcactt gctttcttca
    11821 tcagttcata gctatagcga ctttctaagc gtgaattaaa attcaagtat tcagaataaa
    11881 aatatttgaa ttcaaaatac aacgatcgcc ccgaaaaaaa ataatacaca caaggataaa
    11941 atatcgtccc aacttgtaat ttgtaatgtt gatatctttt attaaacttg caattgagat
    12001 cgcaacctgc aatactgaca atgggtaaat gggtgataaa tacctgtcac cttgcgtaac
    12061 agaaaaccaa aataagacat atgttaaaac aatcgagaaa aatacccaaa gtattttatt
    12121 attcctaagt gtaaagacag acagtatagc ataaaaaaca acatcactgc caactttatt
    12181 aattgcttgt tgcaaatcta atgtggcagg acttatacca tgtcgagctt gttctgctgt
    12241 ttgaacttgt agatcaattt cattccatag gtagtgacca aatatatttc ctccagcagg
    12301 actaaaagga tcaccagaga tccaaaaact acggacatac caataaatgc caccaagaat
    12361 tgttgccaag ctaaaaataa gaaatgtctg tatgtttctc tttttcgcta tcaatataac
    12421 aaatatgatg gctccatata tggcaccaaa tagttttacg ccacaagcaa ttccaataaa
    12481 aaaaccacat aaaaaaatta attcattttg tgttttattg ttttctgagc gaatatatat
    12541 aagaatactg gctgcagcaa aacaaagata gcgttaaatc aatatatgca taaccaagat
    12601 gatctttaag tggattgata gtaaatagta ggattgtagc aataatgcct ggataataag
    12661 ttttcgtaat ttcccatgaa cagccaacaa gtccacaggc tattacaaaa aaacagatat
    12721 ttgctaaaaa ctgagctaac cttacatctc ctaactgtaa acctaaagcc attaaaagat
    12781 ctgcattttg cgggaacaga ggaaaacgca aatattgttc aacaacaatt gcatggtgct
    12841 caactaatga acgagccaaa ggtaagtgat acatagtatc atcccaatca ccgggagctc
    12901 gcagggacga taacaatgta accacaaaaa tcaaaacaag aaataaaata acaaacagcg
    12961 tactgaactc aatattattt attgccagcc atctaaattg ctgacgttta ataatgctta
    13021 aaacccaaca caccgtaaaa attaaaaaga tagcaatctt attaaattgc ccgaccatgc
    13081 ccagaaagta aattatcaaa atatttaaag acatccctat gcataggcaa taaaaaataa
    13141 agaaaatttg ttaagtcctg ccaccccctc cttaaaaaaa gatgacaata caatattacc
    13201 aatgacaaaa aatgtcattc caaaagcaaa acaatacaca acatacaaat acaacggcaa
    13261 ttcaatcata gtaaaatact ctatttacat tatcagttta tagttttttt cagaaagata
    13321 aaataatgcc ttcatttcac gtctagttgt tgtatttgaa tgcaaaataa tgccacatac
    13381 aaatgccatt attgacgata acatgatgga ggcagttaaa aaggcagtcg gaaatcttct
    13441 caccaaacct gtatcaatat aatcaacaat gatgggcaat cctaaaatta tagacaaagc
    13501 agcaagtaaa acgccgataa tactaaaaaa gtatagtggc cgttcaagcg agtaaagttt
    13561 aattattgtt ttcagaatct tgaacccatc actccaagtt gatagtttgc tcacactccc
    13621 ttcagatcgc tcgccatatt ttgtattaac ctcaccatac ttcatacgta attctaaagc
    13681 atgaattgta agctcagttt cgatttcaaa tccccttgat agacatggaa aagatttaac
    13741 atagcgtctg gaaaatacac gataccccga caacatatca gtaaaattcc ctttaaagat
    13801 agtctgaaca gtaccagtca acaatttatt tcctcagcga tgcccttttc tgtatgtatt
    13861 ttgatctcca ttctcttgac gacatcctac aaccatatcc aattgatttt ttatgagatg
    13921 gttaatcatt tctggagcag aacttgcatc ataagtgtca tctccatcaa ccattagata
    13981 aatatccgca tctacatctg agaacatacg tcttacgaca ttccccttgc ctttcagagg
    14041 aacagagtga actgttgctc cagcatcctc tgccacttgt gctgtactgt cagtcgaatt
    14101 attatcaaat acatatatag aagcagttgg aatattcgtt ttgaaatcat ttatgacttt
    14161 tgctattgcc ttctgctcat tataacaagg aataattaca gcgatattaa aagagtgata
    14221 cataagcccc tcatataaac tatttagcta atgtgcaagt aattgtgttt ttaacaaatc
    14281 gtttactaac gaaaaatgaa atattttgat gcgcaatatc ctaaaaccaa actaaaaaga
    14341 gaaaaggtga acaatgtaaa tattggagat aaagctaaga gatcaaataa aacgccgaat
    14401 aaataactca tcgcccccat aaaaaatata aatataaaat atctagcctt ggttgcagat
    14461 actttaaatg agcatcttgc atttgcaaaa aaagaaaagg ttgctgcaca tataaaacca
    14521 acaatgttac ttcggccttg accaaaagct aaattattat aacacgcata aaataccccc
    14581 caatgtaaag cagtattgac aattccaatt aaaaaataac taaaaaaaga actggtcaat
    14641 aacttcccta tttttaacat cctttatttt gctccagaag tgaggttatt actaatttgg
    14701 atattttcta tagaaaatac ccataaaact acaacgccta taataaaaat tcccatgggt
    14761 gtaaaaagaa atggattagt tatagaaacg gtaatactga aaaaaagtat taacgacatc
    14821 attaatgatg tttttgtact atttaaaaaa tagcctttca aaaagataag caacatagga
    14881 actaatgata taaatacacc aataacgcct gttttctgaa gtatctcaag aggtactatt
    14941 tcaatcctta atcgatctaa tataaatgaa ccaaatcctc ttccaaacaa gaacgtcgct
    15001 aaatcaacat ttttataata aaaatataaa tcattaaatc taacagagtc agaatcttca
    15061 ctgcgtgatc cgaccatgta aagcataatt ataaatagaa cgctgcccaa tattattata
    15121 atagtattta aatatagctt atgtaattta aaactggcta ataacagtga taatgttgta
    15181 aaaagtaata cacctcttga ttctgtcaat aatatcgcaa gcactattat gagacatttt
    15241 aaatattttt tgtcgacaaa actgataatc gcaccgacac caaaaaagta gaaggatttg
    15301 gaaaaaaaag ccccatcggg acgaaaaaag aactcgctag tcaatgataa gtgttcataa
    15361 attaaattaa aatttaacaa accgaaattc aacaaaatta tgtatgaaat atataacagt
    15421 gatccatata atgcaaatgc attaactatt ttaataaaaa tctcacatga aaatactttt
    15481 aaagtatatc ttagtgtaaa tagatatata agtgacagca taaaaaggta tggttgcaca
    15541 tcaaacagta tatcatttat tttattacca gctaataagc cgacaaagaa gccataaaaa
    15601 ggaaataaaa gcaaccatat aaaaagactg acacattttt gggtgattga aaaattgaat
    15661 gtaactaaat taaaaattaa aagtataaaa ctaaatacaa ataataaata tcgcaaaggg
    15721 aatattccca atggctccag taatcttcca ccaccaccaa taatcagttc aatacataaa
    15781 aatgttataa aaattttatt tatattattc atattcgtaa ggtgatgttt tttaaaatat
    15841 gtttaaaatc aacattaata aaatataaat tttatcagcc tttttccgcc ttaacttcca
    15901 aaaacttaaa gccaagatca aattgcctat ataaccttta cccaattgat tattcacaaa
    15961 gctatcatca gctaaggcat tagctatttt agttacttct tttcgatacc acttgtttct
    16021 caccaaacca agccgtttgt attttgcttt aaaactaatg tttgctccca cttggttgtt
    16081 ttcatgctgt ctgtacagca ttgttggttt tctatcaata taccacgaat aatttctcgt
    16141 ccgcgcaaat gaatataaaa accaatcatg cagacagata tcttgtgcag catttttatt
    16201 atttatgatg aacttcttaa attcaataag cgtttctttt tttataacat atgtacaccc
    16261 cggacctgct gcttcaaaaa aataatcgaa ttgagtttgc gactgagcct tatcaactaa
    16321 attttttcgc cctgatggat aatatgcaat tacattgctg gaataacaat tcgcattatt
    16381 aaataccaag cagtcaattg cattttttat tttatcttta taccaaatat cgtcctgatc
    16441 actaaaacag atataatcat agttttcaag atcgacatct ttaattaatc tataaaaatt
    16501 tttacctgcc cccccaaact tattgacgct tggaagtata tttataattg agggatatga
    16561 taattgaaat tcttcacaaa tatttaatgt attatcagtt gataaatcat ctgatataaa
    16621 aattgagatg tctacatctt tttgttcaat aatacttttt aattgttcct caatccagca
    16681 ttcgccatta tacgtagcca taagtacagc tactcttttt tgatcagaac tatgcattat
    16741 aacgaccgcc cccagtaatt cctcttattc catccagcgt agccaaaata tgttttttca
    16801 tatattcaat tttatttttt tgagttgcga taattaaagt cgtatgaatt aaagaaaaaa
    16861 caacttccct aatagagagt aaaagaggca catgattttt tctaagcaaa agaaatgcat
    16921 ttcttactcg ataataacga cgtactggcg agtgaattgg tatgttaatc ccacagattt
    16981 ttactgactt atccccaata gcatgaacca tttctatatc aggaatgata tgaactaata
    17041 tgccatagtt taagcatcta agacaccatt ctgtatcgac ataatcaata aaaagttcct
    17101 catccatcat tccaacaatc tcaaaaactt cttttgaaac catggttccg gatgagattg
    17161 ttactgaact tttaaacggt gtctgtcctt cagtgacatg aattttctct cgaagaccat
    17221 tttttttgat attacaaata ggataataat gtgacttatc tctatcaata aaaactgggc
    17281 cagctatttt tgcattttca ttatccatat agcaggaaag ttttttgata aactcattgc
    17341 gaatacggct atcttgatca aaaaaaatta tcttctctgc ccctttttca agagaatatc
    17401 gaatcccaat attttgtgct tttgctatac cttcattcct accaagacaa attaaatgaa
    17461 ctttttcaat gtctgctaat ttatctttca aggtgaaatt agtcctatta tcgactacta
    17521 caacgtaaca attttgctcc tttagttcag tgatcagtgc attcagattt ttaagttcgg
    17581 gattatatgt cacaatgaca gcgtaaatat tactattcat tatccaagtg actcagtaat
    17641 tggttaattt tagttttcat atacagaaca cattctagta taaagccatt gtaataacag
    17701 agcatctaaa attacacgta tgctccagga tattgcggca cccagtaacc catatttttc
    17761 gaccataaaa tacaaaaggg ctaaataagg aaccaattca gcacaatgaa tcaaagcagt
    17821 aattttagat tttcctaatg cctggattgc agagaatggt atcattgcca gcgcattgaa
    17881 gaaaaaaccg atcaataaca catttaatat attaaaggaa ttaatttgat aattctcacc
    17941 aaaccatatt ttcaatacaa gccctgaata taacaagcct atgattatca ccggcaaaca
    18001 gattagaaac attaaaagca atgatttatt tacattacgt ttaaattctt taaagtcttt
    18061 gatgttactt aaccttggaa acactgccct cccgattgct gcaggtatta ttcctaattt
    18121 tagaataacc tccgctggtg cagaataaaa tgcaacttta tctgccccca taatatttga
    18181 cactataaat cgatcaaaat acaccatgac cggacttatg atattactta ctgtcatcca
    18241 accgccaaag aaaaagagtc gtttaaaggt tttataacaa aaatgaaccc ccgaaatttt
    18301 aatatcattc ctgacataat aggcagagac taaaatcgaa ataactcttg caaatattaa
    18361 accagcaacc gctgccgaca atgtagcact ataaaaaaca aatatggccg gaattcccgc
    18421 gatgcaagag cttgatatag atttttgaat atttacaatg ccaaattttt catccccctc
    18481 aagaatggct gaccataatt gatttagaat aaataatgga atgcaaatag ccaacagttt
    18541 aaatgctagt tgtatatcac tatgttcaac accagaaatt tttagataat taacaattcc
    18601 atcagagaaa atcaataata aaaaagcacc aaagcatgaa aatagcacca aaaaagatgt
    18661 acttgttgaa attaccttgg ttctttcatg atgattatca cgatgaattg caatttctct
    18721 aataacagag cgagtcaggc ctacatcaaa aattccagca tacccaacaa gtgcaattgc
    18781 aattgtataa acaccgaaac cttcaggccc caaacctcga gcgagaaatc ctagcgaggg
    18841 gatagcaatt aaagttggta ttgcatagcc aaaaaggttc cagacactat tttttattat
    18901 gctcattatt acatttcttt atcatataaa ataatcagca tctacaaatt tttgaccatt
    18961 aatatcttta tcagaaagca atggattttg aaccggccat tctatattta tacttttgtc
    19021 gctccataaa atcccacgct cttgcatttt tgaatagaaa ttatttgttt tgtagacaaa
    19081 ttctgcttca tcactaagaa cgcaaaaccc atgagcgaat ccttccggta tccataattg
    19141 acgcttattc tccgccgaca aatttacacc aacccacttg ccaaatgttg gtgatgattt
    19201 ccgaatatca acagctacgt caaaaacttc accgacaaca cagcgtacca gttttccttg
    19261 agcataaggt tccagctgat agtgcagtcc gcgtaacaca cccttagttg atttagaatg
    19321 gttatcctga acaaactcaa cctttcgtcc tacagcctct tcgaaaactt tctgattgaa
    19381 gctttccata aaaaagcccc gctcatcacc aaatactttt ggttcaaaaa ttagcacatc
    19441 aggaatttca gttttaatta cgttcatttt attaataacc tttaatcatt ttcagcagat
    19501 actgaccata agcatttttc ttcagcggct cggctaatac cttcacttgc tcagcatcaa
    19561 taaagccttt acggtgagca atttcttccg ggcaggaaac tttcagcccc tgacgctctt
    19621 caattgttgc aatgaagttg cttgcttcaa taagactttg atgtgtcccc gtgtccagcc
    19681 atgcataacc acgccccatc atagcgacag acaaacgtcc ttggtccata taaatacggt
    19741 taatatcggt aatttccagt tcgccacgtg cagatggttt aaggtttttc gccatttcta
    19801 caacgtcatt gtcatagaaa taaagcccag taaccgcata gttacttttt ggttcctgcg
    19861 gtttttcttc cagactaatt gccgtaccgt tatcatcaaa ttccaccaca ccgtagcgct
    19921 caggatcatt tacgtgatag gcaaaaaccg ttgcgccact ctctctgtta acagcggtat
    19981 ccattaattt cggcaggtcg tgaccataga agatattatc accaagtacc agcgcacaat
    20041 catcaccacc tataaattct tcaccaatga taaatgcttg cgccagacca tccggactcg
    20101 gttgtacttt atattgcaga ttaagccccc actggcttcc atcaccaagc aattgttgaa
    20161 aacgtggtgt atcctgcggc gtactgataa tcagaatatc tcgaataccc gccaacataa
    20221 gcgttgaaag cggataataa atcatcggtt tatcataaat cggtagcagt tgtttgctca
    20281 cagccattgt cacaggataa agacgagtgc cggaaccacc agccagaata atacctttac
    20341 gcgttttcat ttcaccattc cttttaattc atctcgctct agcatcatga gcgagatgca
    20401 ataatttgtt aaattgccgt ggtcgtaaat aattcgttga gcatacgttt cacgcccacc
    20461 tgccagtcag gcaagacaag cgcaaagttc tgctgaaatt tttcggtatt gaggcgagaa
    20521 ttatggggac gacgtgctgg tgtgggatag gccgttgtcg gcacggcatt aagtttgtta
    20581 agagcaaggt tgatccccgc tctgcgcgct tcttcaaata ccagagcggc ataatcgtgc
    20641 caggttgttg taccaccagc aaccagatgg tacaaaccag caacttctgg tttatttgcg
    20701 gccacgcgaa tggcatgagc ggtgcaatca gccagtaatt cagcccccgt tggtgcgccg
    20761 aattgatcgt ttattacagc caactcttcg cgttcttttg ccaggcgcaa catcgttttg
    20821 gcaaagttat ttcctttacc agcgtatacc cagctggtac ggaaaataag atgctttgcg
    20881 caatgttctt gtaacgcttt ttccccagct agcttggttt caccgtagac atttagcggt
    20941 gctgttgcat ccgtttccag ccatggcgtg tcaccgttgc ctgggaaaac ataatcagtg
    21001 gagtagtgta taacccaggc tccgacttca ttagctgctt ttgcaatcgc ttcgacgcat
    21061 gtcgcgttaa gtaattgcgc gaaattcggt tctgattccg ctttgtctac tgcagtgtga
    21121 gcagcagcat taacaataac atcggggcga atttttttga cggtttcagc cacaccttcg
    21181 gggttgctga aatctccaca ataatcagtg gagtgaacat cgagcgcaat caagttaccc
    21241 agaggagcca gagcacgctg cagttcccaa cccacctgcc ctgttttgcc gaaaagcagg
    21301 atattcatta ctgacggccc tcatagtttt gttcaatcca cgactgataa gtaccgcttt
    21361 tcacattctc aacccaattt gtattagcca gataccattc caccgtttta cgaatcccgc
    21421 tctcaaacgt ttcttgcggt ttccagccca attcgcggct aattttatcg gcatcaatgg
    21481 cgtaacggcg gtcatgcccc gggcgatcgg caacataagt aatttgctca cgataagatt
    21541 tctctttcgg tacaatctca tccaacaaat cacaaatagt gagcactacg tcgatgtttt
    21601 tcttttcgtt atgtccacca atgttataag tttcacccgc tttaccttcg gttacgacgg
    21661 tatataacgc acgagcatga tcctctacat acaaccagtc gcgaatttga tcccctttac
    21721 cataaatagg taatgcctta ccttccagag cattcagaat aaccaatgga ataagttttt
    21781 ccgggaagtg ataaggacca taattgttcg agcaattagt cacaatggtt ggtaaaccat
    21841 aagtccgctt ccaggcacgt accaaatgat cgctggaagc tttagacgca gaatacgggc
    21901 tacttggcgc gtaagctgtc gtttccgtaa atagcggtaa cgcttcgtta ttatttgctt
    21961 catccggatg gggtaagtca ccataaactt catcagtaga aatatgatga aagcggaaac
    22021 tttttttctt ttcatcattc agagcagacc agtaattgcg cgccgcttct aaaagaacat
    22081 aagtccccac aatattggtt tcaataaatg ccgcagggcc cgttattgaa cggtcaacgt
    22141 ggctctctgc cgccaggtgc atcaccgcgt ctggctggtg ctgtgcgaaa atacgcgaca
    22201 tggctacagc atcgcagata tctgcatgct caaaagcata acgttcagaa tcagaaacat
    22261 cagcgagtga ttccaggttc ccggcgtatg ttaatttatc gacattaaca acactatctt
    22321 gcgtattatt tattatgtga cgaacaacag cagaaccaat aaaccctgcg ccaccagtaa
    22381 caagtatctt cactttttta ttccatatag ctatagagca tgctgtgaaa tagactgctc
    22441 cagatttgat taatagaggt attaatacac gctaccgccc ctggcttaac agctaccagt
    22501 gcactgcatg catctcaacg atgtggcgag cgttagccac tcgcgccaaa tccgaaaaat
    22561 tcaaacgtta attgtcttac caaacagccg tagaaacaag gaaaatcctg gaaaaatttg
    22621 aataatatga gctatgtaac ttcttgtttt tataggcttt ataaaaacgg caacggcagg
    22681 aaaattcaat gagcaaaact tatgccacga atcttcactg ccgttataat tttcttatca
    22741 accgttacat ccggtcagat tttcattatt cgcttaacag cttctcaata cctttacgga
    22801 acttcgcccc ttctttcagg ttgcgtaggc catacttcac aaacgcctgc atatagccca
    22861 tttttttgcc gcagtcgtaa ctgtcgccgg tcatcagcat tgcatcaacg gattgttttt
    22921 tcgccagctc ggcaatagca tcagtcagct gaatacgtcc ccatgcacca ggctgagtac
    22981 gttccagttc cggccaaata tcggcagaaa gcacatagcg acctacggcc atgatgtctg
    23041 agtccagcgt ctgcggctga tccggttttt cgataaattc aacaatgcgg ctgactttac
    23101 cctcacggtc cagcggctct ttagtctgga tgacggagta ttcagagagg tcacccggca
    23161 tacgttttgc cagcacctgg ctgcggcccg tttcgttgaa acgtgcaatc atggcagcaa
    23221 ggttgtaacg tagcgggtcg gcgctggcat cgtcgatcac aacgtctggc agtaccacga
    23281 caaatgggtt gtcaccaatg gcaggtcgcg cacacaaaat ggagtggcct aaacctaaag
    23341 gttcgccctg acgcacgttc ataatgatca cgcccggcgg acagatggac tgtacttccg
    23401 ccagcagttg acgcttcacg cgctgctcaa ggagtgattc taactcataa gaggtgtcga
    23461 agtggttttc gaccgcgttc ttggacgcgt gagttaccag gaggatttct ttgatccctg
    23521 cagccacaat ctcgtcaaca atgtactgaa tcattggctt gtcgacgatt ggtagcatct
    23581 ctttgggtat cgccttagtg gcaggcaaca tatgcatccc gagacccgct acaggaataa
    23641 ctgcttttaa attcgtcatt atttcatcca cctgtaaaat ggttgctgaa ttatagcttg
    23701 ttcgattttt ttcgccagca tcaattaccc tgaattgatt actgaattac ttgtgatgtt
    23761 acgccgcttc gttgtggatt gcagtagcat tgttcctaag tatggctcca tttttccagg
    23821 aatggtcgca aatctactcc ctccgttccg gcaatctaaa gttaatcttt tccacattaa
    23881 caatatggtg attaatcctg tcgatatcga cggagctttg ccctctttca ttcaccgcat
    23941 gaacatttgc aagagacagc agtgtttctt ttttcgccat aaagacgccg cgaacgtctt
    24001 tgcgcatgtc gaagttcatg ctcaatgctg gtccaactga ggattcctgc atcacattga
    24061 tatttcgcat aaaaagatgt tgcggcttgt tgtgtaactc cagcgaggca cgcttcatct
    24121 caatgttagt tagcgacaca aaggagacgg cattcccggc ggagatttgg atgccgcgca
    24181 atttataagc aagatgggta ttatccagtt gaatattatt cactcggaaa ttttgcggta
    24241 tcgagagata tttgccttta attaccccat agccgattaa catcccggcg ctattaatca
    24301 tttcaatatt atcaatcacg aaattgtcac aaccgtaaat agcgactgtc gcgttatcaa
    24361 tgcccgcttt cttactgaaa tccggcgtga tattgcgggc attgatatta cgaataacaa
    24421 aatgtttacc attttcaaca tggatcaact gccgacaatc cgatcccgtg atattcgcca
    24481 cgacaaagtt tttcactgcc tggtcttccg ggtagttgtt atcataagtg cttcccgcaa
    24541 ggcctatgcc gatgccccag ttgattttgc cgttggtaca gttgatacgc tcgatgacat
    24601 ggtcagatat caaaatatca ctgtcgttaa ttgccacgtt ccattcaatg gcgtcgccct
    24661 gtaagtcgct gaacttacag ttggtaatgt tggcaccgat aatctggtta tgaaatccct
    24721 ggcgtaagat ggcgtaatta gcgtggctga cagtgaggtt atcgatggtc aggttgcgca
    24781 tgacccgttt gtttttgccg ccaatataaa tctgcattac cgggccaaag ccgctcatcg
    24841 ccagcccttt gatggtgcag tcagaaccac gcacatccag ggtaatgtta tgcataccgc
    24901 cgccctctcc ccctgtcacc tggctgccgt cctgtaagac aaatcgccct ctgccgttgc
    24961 cgcgcaggct tccaaggatg tgtaacgttt tgccgggagg gatgaagatg ccggtgttga
    25021 tattgtcaca aacgaatccg gcaggcacga cgaccgtttg cccttcgctg aaggcttgtt
    25081 taaatgaggc gatccagtca tgtgggttgt agtcgttaat gttaacgctt tgtcgggcgg
    25141 gaagtgcgcg agcgaaaggg gtatggagga aggcaagcgc cgagcttgcc gtcaggaagg
    25201 tgcgtcggga gagtgtttta aatggcatac gttctcctct ataaagcctg cagcaagctg
    25261 gcgagttctc gattaatcac ctgctggtta aaatcgtgtt cgactttttc gcacgcacgt
    25321 ttgacgaccg gagccagttc gtcggtatcc agttggctaa acgccgccag acgttgtgcc
    25381 agtgcgcgag catcgttctc aggcagcagc cagccggatt tatcggcctc caccagttca
    25441 ggaattccgc tatgcagagt agaaaccacc ggaataccga ccgccatcgc ttccattagc
    25501 gccaccggaa tgccttccat atcgccatcc gcgcccgtta ccgatggcaa caggaagaca
    25561 tccgcgtcgt cgagcatctc cttcacttca tggctcggtt taaagcccgg catctctacc
    25621 acatcttcca gttgatattg ttcgatgatg gtgcgcaggc gtcgttccca caggccaatg
    25681 ccgaggatgc gatagcgaaa tgccacgccc tgctctttca actgacggca ggcttcaatc
    25741 gccacatgca ggcctttttt ctcggttaag cgtgcgacag atatgatttc cagcggcgtt
    25801 gcgggcgctt tcaccggacg cgggctaaag cgcgtcatgt ccacgcccat acgcgatacg
    25861 gcgatttttt ctctggggca gcccattttt tgcagccttc cggcccacag atcgcttatc
    25921 ggtaacatca ggtcgccacg gcgaaacagt tgttgatatt cgggagtgta gtggttgagc
    25981 acttcccgac tggagatatc aataccgtgg aagatggtgg caattttgcc gcgaatgata
    26041 cccagttcgc gtagttttgc tgcggttacc cccgccgggc caaaatgagc gatgaacaca
    26101 tcggcgcgaa acggtgttgc gacctgacca caaatggcag acaaaatcag gttccgcgac
    26161 tcggcaccat agcgtttgag gttgagcgca tgccaggtat ttttacgatg aatgccacgt
    26221 aaggtctggc tggcgcggtg gcgcagtttc gctactttgc ccgcaggttc gtcctgtaac
    26281 cagcgggttc tggcgacaag gttgtatttc gtccatgccg cgtgggtgtt ctctgtatcg
    26341 cctttttgca aagcgacaat ctccacttca aagcccatat caataaacgc ggtaatttga
    26401 ttgaggacga aggtttccga cgacagcggg aatttcaata agaagaagcc gaccttcatt
    26461 tcacctcccc gatgcgctca agcacagact gcaccatctg cattcccgtc tgacgttcgc
    26521 gactgacggc ttcgttaaat cgagcgttca gcaccggaag ctggcctaag gtatccgcaa
    26581 ccatcgcttg caggctgccg tccagtaaat gacgaatatc aatcgccatc tctggcagtc
    26641 ccagctgctg cataatcccg gcggatttat gttcatagtt gatggcaatc gccggagtag
    26701 caaagttcat tgagataatg gcggagtgca ggcgcgtacc gacggtgagt tcacaggctc
    26761 cgagaatttt gcccatttcc agatcgttga gttcatccat cactacgtgg taacgggcag
    26821 gatcgctgat gtgctggcgc agattgagcg ccaccatgcg gtcgtctttg ttatagctgt
    26881 cgatacccgt acaggtggag agcgcaatca cctgatagcc ttcatcgaga atgcgattga
    26941 ccaccccggc aaaggctttt tcatacgctt gttgagtggt gccgagacgt ttgtcgaacg
    27001 gtgccagttc gcgcagggta attgccactg ttttctgttg tgcggcaacg tccagccagt
    27061 gttgaacggc atagctggcg gtgaagtctt cggtgtggtg atcgaccagc cacgcggtat
    27121 cgacgccatg ttccactttt gcggtggtga tatttctgcg tttcatcaaa tcaaggctga
    27181 ccgattcgcg caggatcagc gcgtcgcagt gaccaaagac atagttcgcc agttggttaa
    27241 attgctcatc ctggaagggg ccgacgctgt gaccaatcat aaacagcggc tttttcgcca
    27301 taaatgtgca aagcgcatgt tcaaactgcg gcacgccgta gagatcgaca aaaaacgatc
    27361 cgccgacctg aataatggcg tcgtaacctg acagcaggcg cacgaagtcg gtgaatccct
    27421 gggcgatggc gatattgcgc agcttgccag tgtcggtgac gcgtgagagc aatacctgat
    27481 gctggtagcg gcgacggagg acttttttaa tgcgcccgac aacgcccgcc gcgctattgt
    27541 gttgtgtcat ctgcaggaac agcggatcgc ccattaccgg gcggttgagc agccaggaag
    27601 aactgaccgg atagcggctc atcacgtcca cttcggtatg tggattgagg atgttgatgg
    27661 catcaagtaa gccgcgcaag atggcgctgt cgccacgatt gccgcaagtg tggttgccca
    27721 gaataagtaa tttcattttt tcctcataaa tttgatgcca ggtgaggctg tgtttgttgt
    27781 gttcgttgcc gttttttgcc tgatgcgacg ctggcgcgtc ttatcaggcc tacaggtccc
    27841 gagcacagaa ccgtaggacg gataaggcgt tttacgccgc atccggcaac cgttgtcgga
    27901 accgaaaaca gcaattcacc ccgcccgtaa aagcattttc attttttcgc tgcgacaaaa
    27961 ctgacgcttc acttccacca ccagcggatg gcgtgacagc acaatcatca ccacaaacgc
    28021 cagcacgccc gcggctattt gcaccgccag cagcatcccc agcgccagtt gccctttcag
    28081 cacaatgccc agcgcataac tgaccaccag cgtcggcaac gagagataaa acggcagcca
    28141 caaactcagg atgtactggc gataactgga accaagaacc ggtttaatca tcacgaagta
    28201 actcagaatg gtgttgataa tctgcaccag caggaaaccg agcgtgacgc cgattgcgcc
    28261 cgccatctgc ccaccaataa caatcgccgg aataaacaga aatgttttga atacgttgaa
    28321 tttaaagctg atatcgaccc gcgctttcgc catcagcagc gaaccaatcg ggttacccac
    28381 ggagcgcagc agacccacaa tacacagcaa ttgcagcacc ggaataatgc tgttccactt
    28441 ttcgccgaat accagcggta cgaagttatt cgacaccacc atcagcccca gcagcgccgg
    28501 aaagttgata atccccacta tcgacagcag cttgtagaag ttaacacgca gcttttcggt
    28561 gtcgtcctga attttggcaa acgccggaaa caacacgcgg gtgatgattg ggttcagctt
    28621 catcggcggt acgacggcca cgttgtacgc caggttgtat ccccctgcca cgcccgcgcc
    28681 aagaatacgc gccagcacca gcgttgaaag gttggtgttg agatagttga tgatgctgtc
    28741 cgccgtcagc caggcaccaa aacgtaagtt cggtgccaca gacgccagcg agaagtgcag
    28801 accggggcaa taaattttgc gtccaaagta gccaaacagc agtgttctca ccgcactatt
    28861 gaccagataa ccgaggatcg cggtcatcgc cagcggccag aaatgggcgc taactaccgt
    28921 aaaagtgaag cccgccagca ccgcgctggt ttcgatcatg ccgattttgt tgaactccag
    28981 ctccttctgc atcaacgcgc ggaactgttg cccgtggggg atcaccacaa acgccagcga
    29041 caatgtttta atcaacggtg ccaggtccgg gttattcagc acgtcgccga tgacatcact
    29101 caacaaaaac accgccacac ataccacaag ccccagcccg acgttcagcc agtacagcgt
    29161 ggtgagttca aggtgactga tttcttttcg ctgaataatc gagttagcga taccgaagtc
    29221 agaaagcgta tctgccagcg cgataatcac cagcgacaca gtcagcaggc cgaactggtg
    29281 gttgtcgata atccgcgcca gcacggtcat ctgcaccagc ccgaggccga tgatgatcac
    29341 cgtggcaata gccgaccact tcgcgccgct gatggttttt tcacgtaagc tcatatcaat
    29401 atgccgcttt gttaacgaag cccttgaata ccgtcaggaa aacgattttg atatcgaacc
    29461 agacgctcca ttcgcggatg tactcaaggt cgaactcgac gcgtttttcc attttctcca
    29521 gcgtgtcggt ttcgccgcgc cagccattaa tctgcgccca gccggtaatg cccggtttca
    29581 ctttatggcg cagcatgtag ccttcaatga gctggcgata ctgttcgtta tgcgctactg
    29641 cgtgcggacg tggaccgaca atcgacatcc ccccggtcag cacattgata aactgcggca
    29701 attcgtccag cgaggtgcgg cgcagaaagt tccccacttt agtgacgcgc ggatcgttct
    29761 gcgtcgcctg ggtcaccact ttgtcgttct ccatcacttt catggaacgg aacttccaca
    29821 ctttgatcgg cttgccatcc ataccatagc gagtctggcg gaaaataacc ggccctggtg
    29881 aactgagttt caccgccagc gcaatacagc acagcaccgg ggagatcagc agcagaataa
    29941 gcgtcgccag cacaatgtct tccgcacgtt ttagcaggcg gttaaccccc gaaagcggcg
    30001 tgtcatacag cggcaccacc ggaacgccgt tcatctcttc gagacgtgaa tggagaatgt
    30061 tgaaggtaaa gacgtcgggg atcagcagca ccgaacaggt ggtatccgcc agttgatgga
    30121 ccagtttttt cactcgtgcg ccgtcgcaca tttgcatcgc gatatagacg ttatgaatct
    30181 tgcccgcttt tgcatcctcg accagctgtt gcaggttacc cgcccagtcg ttagaaacgc
    30241 cgcccggttt cgggtcgtgg taaacgccca ccacttcaaa ccctaaccac ggctgattac
    30301 ggaaactctc catcagcatt tgtccggcgg ctaaatcccc cgctacggcg accatccgct
    30361 tgttgtagcc atgattacgc agccagcccg ccccaatgcg aatacatgaa cggcaaacca
    30421 ccagtccgat gctggtcagc ccataccacg ccagccagat tttcagttgc gtgtcgaaat
    30481 cattgttgaa cgccaccagt ccggcgctga aaatcacgct taaggtccag ttttgtagca
    30541 acagggcaaa ttctgtcgct gcccgaacac cgcgccatga gcgataaaaa tcggtgatgc
    30601 cgcccagcat ctggaacacc accagcgtaa tcagcgccac caacaggtgc atgtagagga
    30661 atgacagtcc gctgacttcg caaaccagcc atagcccggc aaacatgatg gtgatatctg
    30721 aaaagcgttg caccatagag attaacgatg cattggtttt cgctcgctcg cgctttttta
    30781 gatttgtcat cgttgttcct gttattagcc ccttacccgc agtggggtaa gggaagatcc
    30841 gacattactc gttcagcaac gtcagcagag ttcgcgttcg cgcttccatc agcggcacat
    30901 caccgcgcga ttccacattc aggcgcacca ccggttcggt attggaggtg cgcaggttaa
    30961 agcgccagtc ggcaaaggtc atgctgatgc catcgctgcg atccaccgcc agcgcctcac
    31021 ggctaaaatg ctgttccacg cggttaatcg cctcaacggg ttgcgccagt ttgctgttga
    31081 tctcaccgct tgccggaaac gccgccatcc ggtcgcgcac cagttcgccc agcgtttttc
    31141 ctttcaggca caccagttcg gcgaccagca gccacgggat catgccgctg tcgcagtaag
    31201 cgaaatcacg gaagtaatgg tgggcgctca tttcgccacc gtagatggcg tcttccttgc
    31261 gcatccgttc tttaataaag gcgtgtccgg ttttcgacat taccggcgtg ccgcctgcag
    31321 cagtcaccac atcaatagtg ttccaagaga gacgcggatc atggatgatc ttcgcgccgg
    31381 gatttttttc gaggaacgct tctgccagca ggccgacaat gtagtagccc tcgataaact
    31441 gccctttttc gtcaaacaga aaacagcggt caaaatcgcc atcaaaggca atgcccatat
    31501 ccgcgccgtg tttgatgacc gcattgcggg tgtcgtcgcg acattccggc agcagcgggt
    31561 taggaatacc gttggggaaa ttgccgtccg gtgtgttgtg tactttgatt aattccacgg
    31621 gcgcgccgag ggctttaaag cgggcttcaa tggcgtccac caccggaccc gctgcgccat
    31681 tcccggagtt gatcaccagc ttgagcggcg tgagattttt gacattgata taaccgaaca
    31741 ggtgatcaac gtaagcgtca cgcaggttga tttgctgata gcgaccgcgt ttggtttcat
    31801 cgacgggagg aaagtcgttg gcttcagcca gacgctggac gtcgcgcagt ccggtatcac
    31861 cgctgatcgt gcgagccccc tcgcgcacca gcttcatgcc gttgtaatcc atcggattat
    31921 ggctggcagt aacttcaatg ccgccatcca cgccgagatg gaacgtggcg aaatagatct
    31981 cttcggtgcc ggacataccg atatccagca catcgacgcc cgcatcctgt aaaccttttg
    32041 ccagcgccag ttttaaggtt tcgctggtga gacggacatc accgcctaac acaatggttt
    32101 tcggtttgag aaattcgcca taggcgcgac caatgcgcca ggcgatatct tcattcagtt
    32161 cttcgcctaa tttcccgcga atatcatagg ctttaaagca ggttaatttt ttcatgtcgt
    32221 tacccttttt caggcaatag ttggccctga ccgaaacggc caattttatt tttgtcgtta
    32281 tttacaccgc ggtttcgcat tcattgcctg atgcgacgct gccgcgtctt atcaggccta
    32341 caaaaccgaa ccgtaggtcg gataaggcgt tcacgccgca tccgacaaac agcgcctgat
    32401 gcgacgttta cacccgtccg tagcgatccg cgaagcgcac cacatcatcc tcttcgagat
    32461 aagagccgga gcgcacttca attaaatcga gcggaatttt ccccgggttt tccaggcagt
    32521 gcgtcgcccc cagcggaata taaatggact cgttttcacc aagcagtttg atatcaccat
    32581 caatggtgac ttttgccgtt cccgcgacca ctacccagtg ttccgcgcgg tgatggtgca
    32641 tctgtaccga caaaccctcg cccggtttca cggtgatgcg tttcacctgg tagcggtcgc
    32701 ccgcgtcgat agagtcatat ttgccccacg gacgatacac ttcgcgatgc acccgatgct
    32761 catggcgacc atcggctttg atctgctcga ccactttttt cacatcctgt accgcgttac
    32821 ggtcggcaat cagcaccgca tctttggtct gcaccactac cagatctttc accccgacgg
    32881 tggtgaccag gcctgattcg gcatacacat agctgttttc agttttgtga ttaatcacat
    32941 cgccgtggca aacgttgccc tcggcggtgt gggcgctgat ctcccataat gaagaccagg
    33001 aaccaacatc gctccagccc gcatccatcg gcaccacaac agcatctgcc gtaggttcca
    33061 tgaccgcgta atccaccgac tcttccgggc aggcgagaaa cgcttcttca tccacacgaa
    33121 taaaatcgag atccggatcg acggcgctca tcgctttttc gcaggcatcg aggatatccg
    33181 ggcgatattt tttcagttct tcgagatagc gtccggcgcg gaacaggaac atgccgctgt
    33241 tccagtaata ttcgccgctt gctacatagg cctgggcggt ttccagattc ggtttttcga
    33301 caaactgcgc cacttcaaag gccaccgtat cctgctcacc cacagacact tcaccgcgac
    33361 gaatatagcc ataaccggtt tctggtagat ccggcacaat gccaaaggtc accagcttgc
    33421 ccgcttcggc ataaggcatg gcattacgca cggcggcacg gaacgcgtct tcatcggcaa
    33481 tcacatgatc cgccgccaat accagcatta acgggtcgct ctccgggcta tgacgttttg
    33541 ccgccagcgc cgccagtgca atggcaggtg cagtgttacg ccctgccggt tcgagaataa
    33601 tgttctcggt gagtttgttc agttgacgca gctgttccgc gacaataaag cggtgctgct
    33661 cattgcaaat caccaccggg ctttcgcact ccacgccgtt caggcggcag atggtggttt
    33721 gcagcatggt gagatcgcct ttcaggcata aaaactgctt ggggtaaagt acgcgggaaa
    33781 gcggccataa acggctaccg gagccacctg ccatcacaac tggatagagt ttcgactgcg
    33841 ccataattat ccccgaatat catttataaa ttgacgtaac acgttctctt tatcgagcgt
    33901 gcgttcggca tattcacgtg ccaccgtgtt atgtttgggc agcaggagcg cctgacgaat
    33961 ccccgccacc agcgcctcga ccgattccgg ttctacgcaa accgcaatac ccggaaaggt
    34021 ttcgcaaagc tgccccagtt ctgtgtgggc ctctgcggta atcaccgcgt taccgcctac
    34081 cgccagaata ttggtcagtt tcgacggcaa tacggcatct gccgcgccgc gtttttgcac
    34141 caccagatgg caatcgccca tcttcagcag tgcaggtaaa gcgtcatacg attgcagcgg
    34201 gaaaaattgc atgttgcgca gtccacgctg ctgcgccatt ttttccagcc gcgctttgcc
    34261 gccgccctgc ccgacaatgg caaaaatcag cggttcatcg cgcaggagat cggcagcttc
    34321 aataacgttt tccagcccct gcttttcacc aatattgccg gagtaaagaa tgattttttt
    34381 gttatccggc aggccaagct ggttacgaag ggcatcaaca tcggcgtctg caacatgctg
    34441 aaaacgggca atttccgacc agttggggaa gaagatgacg ttatccgccg ccacgccttt
    34501 ttcgatggct ttattcatca tcgaacgcga aatcgtggaa acgttatcga cgttatgcag
    34561 tccgctacgt tcgaacgccg ttgccagctg tgccactttg ccgcctttgc cttttccggc
    34621 aaggcccagc cccagcatgg cgtccacttc gtaatcctga atatgcagca cggtacgcgc
    34681 accagagagc ttcgccagca ggcgcattcc cggcgtgcaa aacagcgttg gcactacgcc
    34741 aataatgcga tccggcttcc agcgacgttg cgccatcagt ggaaagaaac tgctgacggc
    34801 aaaactgccg agatgcaaca agcgtttcag ggtgctcggc tgttttggca cgtacagcgg
    34861 gcagcgccac accgtggcgg ccccttcttc tcgtttgtag cgccaggcgg aatagttctc
    34921 acccacctgc cattgcgggt agtaaggcgg tgcggtaatg acccgcacct catgaccttg
    34981 tgccgccaac cattccacca tctcgccggt gtatttgccg atgccggtta actccggcga
    35041 gtagttaatg ccgtagacca gtattttcat aatccgggta ctccggcacg cttctcagcg
    35101 agaaaatagg ctcggctgtt agcgtgaaca tcgttactgg cgagcagcgc gtccggcgtc
    35161 agccagcggt aatcgtcatg ctgctcatcc ggcagtaaca gctcttcttc cgctactctg
    35221 aagcgaaaac cgagcaccac atagtgagtg gtgaaatccg tgccggagaa gttatcgtca
    35281 taaaaatgct gccagacacc gtaaaactgg cctgctgtta tcggcagacg cagccccagt
    35341 tccgccatcg tcagccgctc aaatgcggct tccagcgttt cgtctttctg cacgcgccct
    35401 cccggcacaa accagtaacc ctgcgccggg cggttggttc ttttgccaag cagaaactcg
    35461 ccgcgactgt tctcgacaat aaagtcgaga gagacaagcg gtgtggagcg cactacagtg
    35521 gcaaagtctt cctgacgtaa aaacatcatt ccccccgaaa gcggtcttga ttctcaagga
    35581 accactggta agtgctggca agccccgctt ccagtgagat ttcgtgatac cagccaagct
    35641 gatgcaggcg cgtcacatcc agcagtttgc gcggcgtacc atccggtttg ctggcatcaa
    35701 aaaccacccg acctttgtaa cccaccactt tggcgatggt ttgcgccagc tcgcggatag
    35761 tgcagtcaac gcccgtgccg acgttaatgt gcgacagcat cggttgggta ttctccagcc
    35821 agacttcatg cgccagctcc atgacatgaa tgctcgccgc cgccatatca tcgacgtgca
    35881 ggaattcacg catcggtgta ccgctgcccc ataccaccac gtccggcgca ttctgtgccg
    35941 tcgcctcgtg gaagcgacgc agcaatgctg gtatcacatg ggagttactc gggtggaagt
    36001 tgtcatgcgg cccgtacagg ttggtcggca tgacagtgcg gtaatcgcgt ccatactggc
    36061 ggttgtaaga ttcgcacagt ttgatcccgg cgattttggc aatggcgtaa ggctcgttgg
    36121 tcggctccag cgtgccctgc aataactcgc tttctgccat cggctgtttt gccagtttcg
    36181 ggtagatgca ggacgatccg agaaacatca gtttgttcac gtcgttctga tgcgcggcgt
    36241 gaatgatgtt gctctcaatc atcatgttct ggtagatgaa atccgccgga taggtgttgt
    36301 tggcaacaat gccgcccact ttcgccgccg ccagatagac ctggtcaatg cgttcgctgg
    36361 caaagaaatc atgcacggcg cggctgtcca gcaggttcag ttcatcgcgg gtacgtaata
    36421 ccagctccac atcaccgcgc tgttcgagct gccgcttgat ggcagaaccg accatcccac
    36481 aatggccagc gataaaaatg cgttgtttac tcatgcttat gactccagcg cgatcgccac
    36541 gtcgtagccg tgagatttca gcagagagtg ttttttcgcc gcttcgaggt cattagccac
    36601 catttcagac accatctctc tgagggtgat ttccggtttc cagcccagtt tttcgtgcgc
    36661 tttggtcggg tcgccgagca gcgtttcaac ttctgccgga cggaagtaac gcggatcaac
    36721 ggcgataatc acatcacccg gtttaacgcc cggcgcgtca tgcccggtga cggaaaccac
    36781 aatgcccttc tcttcaacac ccgtgccttc aaagcgcagt ttgatgccca gctgtgctgc
    36841 cgccatttcc acaaactgac gtacggagta ctgaacgccg gtcgcgataa cgaaatcttc
    36901 cggctgttcc tgttgcagca tcatccactg catttttacg tagtctttgg cgtggcccca
    36961 gtcacgcagg gaatccatat tgccgaggta caggcacgac tccagcccct gggcgatgtt
    37021 ggcgattgcg cgggtgattt tgcgggtaac gaaggtttcg ccgcggcgcg gggattcatg
    37081 gttgaagaga attccgttac aagcgtacat gccgtaggat tcgcggtagt taacggtgat
    37141 ccagtaggcg tacagtttgg cgaccgcata cggagatcgc gggtagaacg gcgtggtttc
    37201 tttctgcgga atttcctgca ccagaccata cagttcagag gtggaagcct gatagaaacg
    37261 agttttcttt tccagaccga ggaagcggat cgcctccagc aggcgcagcg tacccatcgc
    37321 gtcgacgtca gcggtatatt ctggtgactc aaaagagacc gcaacgtggc tcattgcgcc
    37381 taggttgtac acttcatccg gctgtacttc acgcaaaatg cgcgtcaggt tagaggtatc
    37441 actcaggtcg ccataatgca gatggaattt cgggttgcag gtgtgcggat cctgataaat
    37501 gtgatccacg cgctcggtgt tgaatgacga tgcgcgacgc ttaataccat gcacctcgta
    37561 acctttttcc agcagaaact ctgccaggta agaaccgtct tgtccggtta caccggtgat
    37621 gagagcgact tttgacatgt cttattcctc tgtatttttg aatttattca gtttcaacgc
    37681 gttcgcgtat caccactgcg ggattccccc ggcaaaccac atttgccgga agcgatttaa
    37741 aaacactgct tcgtgcaccc acgacggtgc cgtcgccgat tgtgacgcct ggggcaacaa
    37801 agacatcggt tgccagccag catttctcgc caatcacaat aggcgtggcg ttaatggtga
    37861 aatgttgact tgcatggtcg tggctaccgg tgcataaata acttttttgc gatatcaccg
    37921 aatgtgcgcc aatggttatt tcaccgaggg tatataaatt gacgtcatcg ccaacccacg
    37981 cgtaatcacc taaggttaat ttccacggat aggtaatttt tactgacgga cgaataacta
    38041 cgttttttcc tatttttgct ccgaataaac gtaataaaaa agcccgccag cgatacaata
    38101 cttgtggcga ccaggcaaat attgttgcct gtactgccca ccataattgc actttaatag
    38161 cgttgccgcc ccggaaccct ttcggcaccg agaaaccg
//
LOCUS       DAJTUI010000029        37990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTUI010000029 DAJTUI010000000
VERSION     DAJTUI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.029"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..1043)
                     /gene="anmK"
                     /locus_tag="OO464_001627"
     CDS             complement(<1..1043)
                     /gene="anmK"
                     /locus_tag="OO464_001627"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6226814.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPG"
     gene            1317..1784
                     /gene="slyB"
                     /locus_tag="OO464_001628"
     CDS             1317..1784
                     /gene="slyB"
                     /locus_tag="OO464_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6226815.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(1831..2265)
                     /gene="slyA"
                     /locus_tag="OO464_001629"
     CDS             complement(1831..2265)
                     /gene="slyA"
                     /locus_tag="OO464_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6226816.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2466..2702
                     /locus_tag="OO464_001630"
     CDS             2466..2702
                     /locus_tag="OO464_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6226817.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2705..3562
                     /locus_tag="OO464_001631"
     CDS             2705..3562
                     /locus_tag="OO464_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6226818.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3562..5574
                     /locus_tag="OO464_001632"
                     /pseudo
     CDS             3562..5574
                     /locus_tag="OO464_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5575..6096)
                     /gene="sodC"
                     /locus_tag="OO464_001633"
     CDS             complement(5575..6096)
                     /gene="sodC"
                     /locus_tag="OO464_001633"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR6226819.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6177..7073)
                     /locus_tag="OO464_001634"
     CDS             complement(6177..7073)
                     /locus_tag="OO464_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6226820.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7122..7361)
                     /locus_tag="OO464_001635"
     CDS             complement(7122..7361)
                     /locus_tag="OO464_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6226821.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7464..8063
                     /gene="nemR"
                     /locus_tag="OO464_001636"
     CDS             7464..8063
                     /gene="nemR"
                     /locus_tag="OO464_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6226822.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8100..9197
                     /gene="nemA"
                     /locus_tag="OO464_001637"
     CDS             8100..9197
                     /gene="nemA"
                     /locus_tag="OO464_001637"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR6226823.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9278..9685
                     /gene="gloA"
                     /locus_tag="OO464_001638"
     CDS             9278..9685
                     /gene="gloA"
                     /locus_tag="OO464_001638"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6226824.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            9788..10435
                     /gene="rnt"
                     /locus_tag="OO464_001639"
     CDS             9788..10435
                     /gene="rnt"
                     /locus_tag="OO464_001639"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6226825.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10528..12642
                     /locus_tag="OO464_001640"
     CDS             10528..12642
                     /locus_tag="OO464_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6226826.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12652..13944
                     /locus_tag="OO464_001641"
     CDS             12652..13944
                     /locus_tag="OO464_001641"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226827.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            14894..14986
                     /locus_tag="OO464_001642"
     CDS             14894..14986
                     /locus_tag="OO464_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR6226828.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(15030..16238)
                     /locus_tag="OO464_001643"
     CDS             complement(15030..16238)
                     /locus_tag="OO464_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6226829.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16246..16746
                     /gene="tnpA"
                     /locus_tag="OO464_001644"
     CDS             16246..16746
                     /gene="tnpA"
                     /locus_tag="OO464_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR6226830.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(16785..17132)
                     /gene="grxD"
                     /locus_tag="OO464_001645"
     CDS             complement(16785..17132)
                     /gene="grxD"
                     /locus_tag="OO464_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6226831.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17467..18294
                     /gene="mepH"
                     /locus_tag="OO464_001646"
     CDS             17467..18294
                     /gene="mepH"
                     /locus_tag="OO464_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6226832.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18422..19003
                     /gene="sodB"
                     /locus_tag="OO464_001647"
     CDS             18422..19003
                     /gene="sodB"
                     /locus_tag="OO464_001647"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6226833.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19149..20318)
                     /locus_tag="OO464_001648"
     CDS             complement(19149..20318)
                     /locus_tag="OO464_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6226834.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20484..20573)
                     /gene="ynhF"
                     /locus_tag="OO464_001649"
     CDS             complement(20484..20573)
                     /gene="ynhF"
                     /locus_tag="OO464_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6226835.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            20872..21897
                     /gene="purR"
                     /locus_tag="OO464_001650"
     CDS             20872..21897
                     /gene="purR"
                     /locus_tag="OO464_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6226836.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(21894..22826)
                     /locus_tag="OO464_001651"
     CDS             complement(21894..22826)
                     /locus_tag="OO464_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6226837.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            22939..24150
                     /locus_tag="OO464_001652"
     CDS             22939..24150
                     /locus_tag="OO464_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6226838.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            24441..25589
                     /gene="cfa"
                     /locus_tag="OO464_001653"
     CDS             24441..25589
                     /gene="cfa"
                     /locus_tag="OO464_001653"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6226839.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(25629..26270)
                     /gene="ribE"
                     /locus_tag="OO464_001654"
     CDS             complement(25629..26270)
                     /gene="ribE"
                     /locus_tag="OO464_001654"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6226840.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            26485..27858
                     /gene="mdtK"
                     /locus_tag="OO464_001655"
     CDS             26485..27858
                     /gene="mdtK"
                     /locus_tag="OO464_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6226841.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(27899..29155)
                     /locus_tag="OO464_001656"
     CDS             complement(27899..29155)
                     /locus_tag="OO464_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6226842.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            29463..29539
                     /locus_tag="OO464_001657"
     tRNA            29463..29539
                     /locus_tag="OO464_001657"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29497..29499,aa:Val,seq:gac)
     gene            29544..29620
                     /locus_tag="OO464_001658"
     tRNA            29544..29620
                     /locus_tag="OO464_001658"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29578..29580,aa:Val,seq:gac)
     gene            29728..30033
                     /locus_tag="OO464_001659"
     CDS             29728..30033
                     /locus_tag="OO464_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR6226843.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            30159..31581
                     /locus_tag="OO464_001660"
                     /pseudo
     CDS             30159..31581
                     /locus_tag="OO464_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(31593..32405)
                     /locus_tag="OO464_001661"
     CDS             complement(31593..32405)
                     /locus_tag="OO464_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226844.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(32409..33194)
                     /locus_tag="OO464_001662"
     CDS             complement(32409..33194)
                     /locus_tag="OO464_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR6226845.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(33191..33859)
                     /locus_tag="OO464_001663"
     CDS             complement(33191..33859)
                     /locus_tag="OO464_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6226846.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(33923..34561)
                     /locus_tag="OO464_001664"
     CDS             complement(33923..34561)
                     /locus_tag="OO464_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR6226847.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(34574..36653)
                     /locus_tag="OO464_001665"
                     /pseudo
     CDS             complement(34574..36653)
                     /locus_tag="OO464_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(36674..37300)
                     /locus_tag="OO464_001666"
     CDS             complement(36674..37300)
                     /locus_tag="OO464_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR6226848.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(37756..37965)
                     /gene="fumD"
                     /locus_tag="OO464_001667"
     CDS             complement(37756..37965)
                     /gene="fumD"
                     /locus_tag="OO464_001667"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR6226849.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         9130 a         9931 c         9305 g         9624 t
ORIGIN      
        1 tttcctggta atcccgccag cgtccgccag gcaagccagg cgaaagccaa tgcttccatg
       61 tcatcgccac taatgccaac ggcatcggtg gtggtgactt ctgtgcctag cagtaatacc
      121 gccagacgcg ccatgagtag cggattacga ctacctccac cacataccat caatcgttcg
      181 cagccaccgc tcaacaaaac ttgttcagaa atggtcacgg cggtgagttc tgccagtgtg
      241 gcctgaacat cacggggatc aacacccgga aaatggcgta aatggcgctc cagccagccg
      301 tagttaaagt attcgcgtcc ggtgcttttc ggtgcaggtt gcgagaaata cgggtcgctg
      361 agcatatttt gcagcagtgg gagaataact ttacccgccc gtgcccactc ggcatcttta
      421 tcgtaaggtt tacctgcctg acgccagatc caggcatcca tcagcatgtt accaggaccg
      481 gtatcgtagc cgccaaccag ctgcccagga atgagcagtg acagattggc gatgccgcca
      541 atattgagca ccattcgtcg ctcggttggg tgagccagca aggcatgatg gaacgcaggt
      601 accagcggtg cgccttgtcc tcccaaggca atatcgcgac ggcgaaaatc accgacaacc
      661 gtaattccgg tgcgtgccac aatttgattg ttatcgccaa tctgtaaagt gtgtggtgcc
      721 acgcccgtcg gttcatgcca gacggtttga ccgtgacagc cgatcgcaac tatatctctc
      781 gcctgcaggt tttgttcctt aagcaaggca ttgaccgcat cagcaaaaag tcgcccgagt
      841 tgagtatcaa gctgtccaaa ctgcgaaagt gtaagctgct ggccctggca aatatccagt
      901 acagcctgtt tcagagacac cgggatcggc cagctcaagc ttgccagctg tgcgacccgg
      961 tgttcatcaa ttgtcgccaa cacaacatca acaccatcaa ggctggtgcc tgacataacg
     1021 ccaataaagc ggcccgattt catagttcat cctttttcaa tctgacgttt gcgcaccact
     1081 caaacataaa cttttcgtga ataccatgcg gaatgaccga tttttaccgt tggtagtaaa
     1141 acattatctt caaatcaata atcatcatga atgttttgtt tataattggt tgatcctact
     1201 ttcattatga tttgctcata tttggtagaa catgtaacca tggattcaca tatgccatat
     1261 actttgacca tgagggatgc ttgcgtggcg tttcatggtg aacaggagat ttttcaatga
     1321 ttaaacgcgt attggttgtt tcaatggtag gtctgtctct tgtcggttgt gttaataacg
     1381 acaccctgtc aggggatgtt tataccgctt ctgaagcgaa acaagtacag aatgtcagct
     1441 atggcaccat cgttaacgta cgtccggtac agattcaggg cggtgatgat tccaacgtta
     1501 tcggtgcaat tggcggtgct gttcttggtg gtttcctggg gaatactgtt ggtggcggaa
     1561 ccgggcgttc tctggctact gcagcaggcg ctgttgcagg tggcgtagct ggtcagggcg
     1621 tacagagtgc aatgaacaaa acgcagggtg tcgagctgga aattcgtaaa gacgatggta
     1681 ataccatcat ggtggtacag aaacaaggca acactcgttt ctctccgggc caacgtgtcg
     1741 tactggccag caatggcagt caggtgaccg tttctccgcg ctaaataagt ttgcgtgtgg
     1801 tcaggttact gaccacacgc ccccttcatt tcaccctttg gcctgtaact caatgatatt
     1861 atgctcaagt tttgcgatga gcgtaatcag ttgctccagt tcctctgcgg agatgccatg
     1921 taatatttcc gcgcgggttt tgttaataac agcttccatt tcgctgatca gtggctctgc
     1981 cttttccgtc agtttaatac gtttagcccg acgatcgctg gcacaagttt gacgcgaaat
     2041 taaccctttt tcttccagtt ggtccagagt acggaccagt gatggctgct cgatgccaat
     2101 cgcttttgcc agttgaattt gcgactggtc tggaggtaac tgatggatat tgtgtaacgt
     2161 aacccaatgg gtttgtgtta actccagcgg tttcaggcga tggtctatca gagcacgcca
     2221 tatgcgcacc aaccgtgcca gatcagaacc tagtggcgat tccaatttca tctccttata
     2281 attagcttgc taagatatta tgcggctttt agaatagtgt gcagcaattg tattgctaaa
     2341 acaaatgtat tgctgcattt ggttaccgtc agacatattt ttcagaaatt gcgcgtaaat
     2401 ttttcgcact taaagaatat ttattaatct aacgcaatat attcggtcgt aaaggaatct
     2461 actttgtgaa gtttatgctc aatgcaacag gattgccctt acaagatctg gtgttcggtg
     2521 cgtccgtcta ttttcctccg tttttcaaag cattcgcgtt tggattcgtc atctggcttg
     2581 tcgtacaccg cctgcttcgt ggctggatct acgccggcga tatctggcat cccttgttaa
     2641 tggatttatc gctgtttgcg atttgcgttt gccttgctct ggcaatactg attgcgtggt
     2701 aactatgtca attaaaacaa ttaagtattt ctcaacaatc attgtagcga tagttgcggt
     2761 tcttgccgga tggtggctgt ggaattatta catgcaatct ccgtggacac gcgacgggaa
     2821 aatacgcgca gaacaggttt ctatcacccc acaggtgtcg ggacgtattg ttgagctgaa
     2881 tataaaagac aaccaactgg ttaacgcggg ggaacttctg ctcacgatcg ataaaacgcc
     2941 ctttcagatc gccgagctga acgcacaggc tcagctggcg aaagcgcaat ctgacctcgc
     3001 aaaagccaat aacgaagcga atcgtcgtcg tcatctctca caaaatttta tttttgccga
     3061 agaactcgat accgctaacc tcaatgttaa agcgatgcag gccagtgtta atgccgcaca
     3121 ggcgacgctg aaacagacac aatggcaact ggcgcaaacg gaaattcgcg ccccggtgag
     3181 tggatgggtg actaacctca ctacccgcat cggcgattac gctgacaccg ggaaaccgct
     3241 gtttgccctt gtcgacagcc actcgtttta cgtcattggt tattttgaag aaaccaagtt
     3301 gcgccatatc cgcgaaggtg caccagcaca aattaccctc tatagtgata acaaaacgtt
     3361 acagggtcac gtttccagta tcggtcgggc gatttatgat cagagcgttg aaagtgactc
     3421 aagccttatt ccggatgtga aacctaacgt cccctgggtt cgtctcgccc aactcgttcc
     3481 cgtccgcttt gcgctggata aagttccagg cgatgtcacg ctggtgtcgg gcaccacctg
     3541 tagcattgcc gtaggtcaat aatgaacgca tcgtcatggt ccttgcgcaa tttgccctgg
     3601 ttcagggcca cgctggcgca atggcgttat gcgttacgca ataccattgc catgtgtctg
     3661 gcgctgacgg ttgcctatta tttgaatctg gatgaaccct attgggcgat gacctcggct
     3721 gcagtggtta gctttcccac cgttggcggt gtcatcagca aaagcctcgg acgcatggct
     3781 ggcagtttac tgggagccat tgcggcactg cttcttgccg gacatacgct caatgagccg
     3841 tggttttttc tattgagcat gtcggcgtgg cttggctttt gtacctaggc ctgtgcgcac
     3901 ttcacgaata acgtcgcgta tgcatttcaa ctggcgggct acacggctgc catcatcgcc
     3961 tttccgatgg ttaatattac tgaggccagc cagctgtggg atatcgctca ggcgcgcgtt
     4021 tgcgaggtga tagtcggtat tttgtgcggc ggcatgatga tgatgatcct gcctagcagt
     4081 tccgatgcta ctgccctttt aactgcattg aaaaacatgc acgcccgatt actggaacat
     4141 gccagtttac tctggcagcc tgaaacaaac gatgccattc gtgcagcaca tgagggggtg
     4201 attgggcaga tactgaccat gaatttgctg cgtatccagg ctttctggag ccactatcgt
     4261 tttcgccagc aaaacgcgcg ccttaatgcg ctactccacc agcaattacg tatgaccagt
     4321 gtcatctcca gcctgcgacg tatgttgctc aactggccct caccgccagg tgccacgcga
     4381 gaaattctcg aacaattgct gacggcgctc gccagttcgc aaacagatgc ttacaccgtc
     4441 gcacgtatta tcgccccgct acgcccgacc aacgtcgccg actaccggca cgtcgccttc
     4501 tggcagcgac tacgttattt ttgccgcctt tatctgcaaa gtagtcagga attacatcgt
     4561 ctgcaaagcg gtgtagatga tcatgccaga ctcgcacgga catccgctct ggctcgtcat
     4621 accgacaacg ccgaagctat gtggagcggg ctgcgtacat tttgtacgtt gatgatgatt
     4681 ggcgcatgga gtattgcatc gcaatgggat gccggtgcca atgcattaac gctggcagca
     4741 attagctgcg tactctactc cgccgtcgca gcaccgttta agtcgttgtc actgctgatg
     4801 cgcacgctgg tgttactttc gctattcagc tttgtggtca aatttggcct gatggtccag
     4861 attagcgatc tgtggcaatt tttactgttt ctctttccac tgctggcgac aatgcagctt
     4921 cttaaattgc agatgcccaa atttgccgca ctgtgggggc aactgattgt ttttatgggt
     4981 tcgtttatcg ctgtcactaa tcccccggtg tatgattttg ctgattttct taactataat
     5041 ctggcaaaaa tcgttggcgt tgcgctggct tggctagcgt tcgccattct gcgtccagga
     5101 tcggatgctc gtaaaagccg ccgccatatt cgcgcgctgc gccgggattt tgtcgatcag
     5161 ctaagccgcc atccaacact gagtgaaagc gaatttgaat cgctcactta tcatcacgtc
     5221 agtcagttga gtaacagcca ggatgcgctg gctcgccgtt ggttattacg ctggggtgta
     5281 gtgctgctga actgttctca tgttgtctgg caattgcgcg actgggaatc gcgttccgat
     5341 ccgttatcgc gagtacggga taactgtatt tcactgttgc ggggagtgat gagtgagcgt
     5401 ggcgttcagc aaaaatcact ggcggccaca cttgaagaat tacagcggat ttgcgacagc
     5461 cttgcccgtc atcatcaacc tgccgcccgt gagctggcgg caattgtctg gcggctgtac
     5521 tgctcgcttt cgcaacttga gcaagcacca ccgcaaggta cgctggcctc gtaattactt
     5581 aattacacca caggcatagc gttcaccgcc accgcccagc ggtttaggtt gatcggacat
     5641 attatcgccg ccaacgtgga ccatcagcgc cttgtctttg atttcatcca gtgatttcag
     5701 acgaggcgcg atgacggcat cggtagcttt gccgtcatta ttgacgacca gtgcaggcag
     5761 atcgcctaaa tgcccggtac cttctggccc ttcatgttta ccggtatttt gtggatcaag
     5821 atgcccgcct gcggattccg cggcgctggc tttgccatct ttggtggctg gctggcagct
     5881 tcctttggca tgaatatgga agccatgttc accggggggt aatgctttca gatcgggtga
     5941 aaactccaga cctttatcgg tttcagtaat ggtgacgcta ccaattgact gcccaacccc
     6001 ttgcgacgtg acgaggttca tctcgacttt ttcactggca gcttgtgcgc cggtcgcaac
     6061 aaccagcgcc agaatggcca gactaaaacg tttcatagga cctccgttca ttgtgcattc
     6121 ctgaataagt gtaaaccagt gacacatttt tgaacggcgg ctattcctaa aagtgcttac
     6181 ggtacgtcgt accccagtgc agctttacgg atacgaaacc attgttgacg ggtcattttc
     6241 agtgtttctg cttcgacagc tgcccgtacg cgctcaattt tacctgagcc gataattggc
     6301 agcggctgcg acggtaaacg taatacccag gcgtaaacca cctgttcaat cgagcccgcg
     6361 tttaactcct ccgccaccac agccagttca tcacgcagcg gctggaaata atcatcatta
     6421 aacagacgac caccaccaag gcaggaccac gccatcggac gaacacgcag ttgttgtagt
     6481 tggtcgagcg tgccatccag cagtaacggc tgatgcaccg gggatatttc cacctgatta
     6541 gtggcaaggg taaacggcag acgtgattgc aacagggcaa attgcgcagg cgtaaagttc
     6601 gatacgccaa aatgacgcac tttgccgctc tgatgcagat gtttgaacgc gtccgccact
     6661 tcatcggcat ccattaacgg gtctggtcgg tggattaaca gcagatccag atgatcggtc
     6721 gcgagattaa ttagcgactg ttcggcgctc ttaatgatgt gattgcggtc agtgatgtaa
     6781 tgaccaatga cgttttcttc acgcgcggtc gtcgcgatac cgcatttact gacgatttcc
     6841 atccgttcac gcaggtgagg tgccagtttc agtgcctcgc caaacgccgc ttcgcactga
     6901 tagccaccat aaatatcagc atggtccacg gtggtcacgc cgagatccag atgctcttca
     6961 ataaaactga ccagctggcg ggcggacata ttccagtcca tcaatcgcca gtagcccatc
     7021 acaaaacggg aaaactccgg gccttccggc gcaatagtaa tacgctgaac cataatcgct
     7081 tcctcttatc agatataaga ggagtatacg caagattagg ttcaaaagag tgatggttgc
     7141 tccggttcgt ctgatgacgc tggcttattt gcgcgtaatt tgcgcattaa tcgctgccga
     7201 caaaggcgca gcacctcttg tttttcgcca tcgctcattt tattccagtt aaaacgctca
     7261 tcccgactac gaaaacagcc gcgacaaaac ccgcgttcgt cagactggca aattccccgg
     7321 cacgggctct ggacgggaaa gaactctaat tgctccgcca cctcgccctc ctcagataag
     7381 attattacca ttattgaagc tgttaatgtc caaagtagca actttgcttg cactagacca
     7441 actggtctac tacactccaa cgcatgaaca aacacaccga acatgatact cgcgaacatc
     7501 tcttggcgac gggcgagcaa ctttgcctgc aacgtggatt caccgggatg gggctaagcg
     7561 aattactaaa aaccgctgaa gtgccgaaag ggtccttcta tcactacttt cgctctaaag
     7621 aagcgtttgg cgttgccatg cttgagcgtc attacgccgc atatcaccag cgactggctg
     7681 agttgctgca atccggcgaa ggtaactacc gcgaccgcat actggcttat taccagcaaa
     7741 cactgaacca gttttgccaa catggaacca tcagtggttg cctgacagta aaactctctg
     7801 ccgaagtgtg cgatctgtca gaagatatgc gcagcgcgat ggataaaggt gctcgcggcg
     7861 tgatcgccct gctctcgcag gctctggaaa atggccgtga gaaccattgt ttaacctttt
     7921 gtggcgaacc gctacaacag gcacaagtgc tttacgcact atggcttggc gcgaatctgc
     7981 aggccaaaat ttcgcgcagt ttcgagccac tggaaaacgc gctggcccat gtaaaaaaca
     8041 ttattgcgac gcctgccgtt tagcaggcat tttttatcac cagacgaccg ggagccttta
     8101 tgtcatctga aaaactgtat tccccactga aagtgggcgc gatcacggcg gcaaaccgta
     8161 tttttatggc accgctgacg cgtctgcgca gtattgaacc gggtgacatt cctaccccgt
     8221 tgatggcgga atactatcgc caacgtgcca gtgccggttt gattattagt gaagccacgc
     8281 aaatttctgc ccaggcaaaa ggatatgcag gtgcgcctgg catccatagt ccggagcaaa
     8341 ttgccgcatg gaaaaaaatc accgctggcg ttcatgctga aaatggtcat atggccttgc
     8401 agctgtggca caccggacgc atttctcacg ccagcctgca acctggcggt caggcaccgg
     8461 tagcgccttc agcacttagc gcgggaacac gtacttctct gcgcgatgaa aatggtcagg
     8521 cgatccgcgt tgaaacatcc atgccgcgtg cgcttgaact ggaagagatt ccgggtatcg
     8581 tcaatgattt ccgtcaggcc attgctaacg cgcgtgaagc cgcttttgat ctggtagagc
     8641 tccactctgc tcacggttat ttgctgcatc agttcctttc cccttcttca aaccatcgta
     8701 ccgatcagta cggcggcagc gtggaaaatc gcgcacgttt ggtactggaa gtggtcgatg
     8761 ccgggattga agaatggggt gccgatcgca ttggcattcg cgtttcgcca atcggtactt
     8821 tccagaacac agataacggc ccgaatgaag aagccgatgc actgtatctg attgaacaac
     8881 tgggtaaacg cggcattgct tatctgcata tgtcagaacc agattgggcg gggggtgaac
     8941 cgtatactga tgcgttccgc gaaaaagtac gcgcccgttt ccacggtccg attatcggcg
     9001 caggtgcata cacagtagaa aaagctgaaa cgctgatcgg caaagggtta attgatgcgg
     9061 tggcatttgg tcgtgactgg attgcgaacc cggatctggt cgcccgcttg cagctcaaag
     9121 ctgagcttaa cccacagcgt gccgaaagtt tctacggtgg cggcgcggaa ggctataccg
     9181 attacccgac gttgtaatcc aacattgcga gcggcgtaaa gccgccgcta tactaaaaca
     9241 acattttgaa tctgttagcc attttgagga taaaaagatg cgtcttcttc ataccatgct
     9301 gcgcgttggc gatttgcaac gctccatcga tttttatacc aaagtgctgg gcatgaaact
     9361 gctgcgtacc agcgaaaacc cggaatacaa atactcactg gcgtttgttg gctacggccc
     9421 ggaaaccgaa gaagcggtga ttgaactgac ctacaactgg ggcgtggata aatacgaact
     9481 cggcactgct tatggtcaca tcgcgcttag cgtagataac gccgctgaag cgtgcgaaaa
     9541 aatccgtcaa aacgggggta acgtgacccg tgaagcgggt ccggtaaaag gcggtactac
     9601 ggttatcgcg tttgtggaag atccggacgg ttacaaaatt gagttaatcg aagagaaaga
     9661 cgccggtcgc ggtctaggca actaatctcc tgccgggcgt gaactcatcg cgcccgcatc
     9721 tttactgcat cgacaagtaa tatttgtcat aatgcgcgct gcaatttatc cgtattaaga
     9781 gaatcagatg tccgataacg ctcaacttac cggtctgtgc gaccgttttc gtggttttta
     9841 tcctgttgtg atcgatgttg aaacagccgg atttaacgcc aaaaccgatg cgctgcttga
     9901 gattgccgcc atcaccctga aaatggatga acaaggctgg ctgatgccgg acaccacatt
     9961 acatttccac gtcgaaccat ttgtcggcgc aaatttgcaa ccagaagccc tcgccttcaa
    10021 cggcattgac ccgaacgatc ccgatcgcgg cgcggtcagc gaatacgagg cgctgcacga
    10081 aatttttaaa gttgtacgta aaggtattaa agcgagcggc tgtaaccgcg ccattatggt
    10141 ggcgcacaac gccaattttg atcacagctt tatgatggcc gccgcagaac gcgcctcgct
    10201 gaaacgtaac ccgttccacc ctttcgccac ttttgacact gctgcactgg ccgggctggc
    10261 actcggacaa accgtattgt caaaggcttg ccagaccgct ggcatggact tcgacagcac
    10321 ccaggcgcac tccgcgctgt acgacaccga acgcactgct gtgctgtttt gtgaaatcgt
    10381 caaccgctgg aaacgtctgg gaggctggcc gctacctgcc gccgaagagg tgtaatcgag
    10441 tcgatgcctg atgacatgca atgattcagg catctatagt gaggctattc cacgcatcaa
    10501 gcatgatatt cacggggaat agcgttaatg gcagataatc cagacccttc atcgctcctg
    10561 ccggacgtgt tttcaccggc gacccgcgac tggtttcttc gcgcctttaa acagccgacc
    10621 gctgtccagt cgcaaacctg gcatgtggcg gcgcgaggcg aacatgcgct ggtgattgca
    10681 ccgactggct ccgggaaaac gctggcagca tttctctacg ccctcgatcg gctcttccgc
    10741 gaaggcggcg aagatacccg cgaggcgcat aagcgtaaaa cctcacgcat cctctatatt
    10801 tcaccgataa aagccctggg caccgacgtt cagcgcaact tgcagatccc gttgaagggt
    10861 attgccgatg aacggcggcg gcgcggcgaa acggtaatta ttgatgaagt ccacgcggta
    10921 gcgggcagta aacgtggtgc gcatctggcg ttaagtctgg agcggctcga tgcgctgctc
    10981 cacacctcag cacagcgaat tggcctttct gccactgtgc gctcagccag cgatgtggca
    11041 gcatttcttg gtggcgatcg cccggttacg gtagtcaacc cgcccacaat gcgccatccg
    11101 cagatacgaa ttgtcgtacc ggtcgccaat atggatgatg tctcatcggt cgccagcggc
    11161 accggcgaag acagccatgc cggccgggaa ggctccatct ggccatatat tgaaacgggt
    11221 atccttgatg aagtgttgcg ccatcgctcg accattgtct ttactaattc acgcgggctg
    11281 gcggaaaaac tgacggcacg attaaatgag ctttacgccg cacgcttaca gcgttccccg
    11341 tctatcgccg ttgatgcggc ccatttcgag tcgacctccg gcgcaacctc taaccgtgta
    11401 caaagtagcg acgtttttat tgcccgctca caccacggct ccgtctctaa agaacaacga
    11461 gcaatcaccg aacaggcgct gaaatcgggt gaattacgct gcgtggtcgc aacctccagt
    11521 cttgaactgg ggattgatat gggcgcggtg gatctggtga ttcaggtggc aacgccgctt
    11581 tctgttgcca gtgggttaca acgcattggt cgcgccggac atcaggttgg cggtgtatct
    11641 aaagggctgt ttttcccccg tacccggcgt gatttagtcg attccgcagt cattgtagag
    11701 tgtatgttcg caggcaggct ggaaaacctg acaccaccgc ataatcctct cgacgtcctt
    11761 gcgcagcaaa ccgttgccgc cgcggcgatg gatgcattac aggtagacga atggtactcc
    11821 cgcgtacgcc gtaccgcacc gtggaaagat ctgccaagac gtgtttttga cgccacgctg
    11881 gatatgcttt ccgggcgcta tccctctggc gatttttctg cttttcgccc caaactggtc
    11941 tggaacaggg agaccgggat attgaccgcc cgacctggcg ctcaattgtt ggcggttacc
    12001 agcggcggca ccattccgga tcgtggcatg tatagcgtgt tattacccga aggtgaagaa
    12061 aaggccggtt cgcggcgggt gggtgaactg gatgaggaga tggtatatga gtcgcgggtg
    12121 aacgacatta tcactctcgg cgctacctca tggcggatcc agcaaatcac ccgcgatcag
    12181 gtgattgtga ctcctgctcc gggtcgttct gcccagctcc ccttctggcg tggtgaaggt
    12241 aacggacgtc cggctgaatt aggcgagatg atcggcgatt ttcttcattt actggcggat
    12301 ggcgcgttct tttccgggac tattcccccg tggctggcag aagaaaatac gaacgccaat
    12361 attcaggggt tgattgacga gcaacgcaac gcgacgggca tcgttccggg gagtcgccat
    12421 ctggtcctcg aacggtgccg tgatgaaatt ggcgactggc gtattatttt gcactctccc
    12481 tatggaagac gggtgcatga accctgggcg ctggcgattg ccgggcgaat acatgcccta
    12541 tggggcgctg acgcgtctgt ggtcgccagt gatgacagca ttgttgcgcg cattcctgac
    12601 actgatggta aattgcccga cgccgcgatt tttttgtttt aaccagaaaa gttgctgcaa
    12661 attgtccgcg aggcggtagg cagctcggca cttttcgccg cccgttttcg cgaatgcgcc
    12721 gcgcgggcat tattaatgcc gggccgcacg ccgggccatc gcaccccgct ttggcaacaa
    12781 cggctgcgcg ccagtcagtt gctggaaatc gctcagggat atccggattt tccggtcatt
    12841 ctcgaaaccc tacgcgaatg tctgcaagat gtttatgatc ttcccgcact ggaacgtttg
    12901 atgcgtcgcc tgaacggtgg cgaaattcaa atatccgatg taacgaccac tacgccctcg
    12961 cctttcgcca caagtttatt gttcggctat gtcgcggaat ttatgtacca gagcgacgcc
    13021 ccgctggcag agcgccgggc atccgtactg tcgctggaca gcgagttact gcgcaatcta
    13081 ctcggacagg tcgatccggg ggaattactc gacccgcagg tcattcgcca ggtggaagaa
    13141 gagttgcaac gactggctcc tggtagaaga gcgaaaggtg aagaaggatt gttcgacctg
    13201 ctgcgcgaac tggggccaat gaccgttgaa gacctggcgc aacggcatac aggcagcagt
    13261 gaagaggttg cgtcgtatct ggaaaatctt cttgcagtaa aacgaatctt cccagcgatg
    13321 attagcggac aggagcgtct tgcctgtatg gatgatgccg ccaggctgcg tgatgccctc
    13381 ggcgtacggc taccagagtc attgccagag atttatttac atagagtcag ttacccgctt
    13441 cgcgacctct ttctgcgcta tctccgggct catgctctgg tcacggctga acaactggct
    13501 catgagttta gtctcggtat tgccattgtc gaagagcagc ttcagcaact gcgtgaacag
    13561 ggtctggtga tgaatctgca acaagacatc tgggtgagcg atgaagtatt tcgtcgtctg
    13621 cgtttgcgct cgctgcaagc cgccagagaa gcgacgcgtc ccgttgcagc cacgacctat
    13681 gcgcgattgc tgctggaacg tcagggcgta ttacccgcca ccgatggtag cccggcgctc
    13741 tttgcctcaa catctccagg cgtttatgag ggcgtagatg gagtgatgcg ggtgatcgaa
    13801 cagcttgccg gagtcggttt acccgcctca ctctgggaaa gccagatcct gcctgcccgc
    13861 gtacgcgact attcgccaga aatgctcgat gaattactgg caaccggagc ggttatctgg
    13921 tcgggacaaa aaaaagctgg gtgaagatga cggcctggtg gcactgcatc tacagaaata
    13981 tgccgcagaa tcgttcactc ccgccgaagc ggatcaggcg aatcgttcgg cgctgcagca
    14041 agcgataatc gctgttctgg ctgacggagg agcctggttt gcacaacaaa tcagccaacg
    14101 gatacgcgat aaaatcgtcg aatcggttga ttcctcggca ctgcaagagg cgttatgggc
    14161 gctggtctgg caaggcgtca ttaccagcga tatttgggca ccgttacgcg ccctcacccg
    14221 cagcagttcc aacgcacgca cctcaactcg ccgcagtcac cgggctcgtc gtgggcgtcc
    14281 tgtctatgca caacccgtct cgccgcgggt atcttacaac acaccaaatc tggctggacg
    14341 ctggtcgtta ttgcaggtgg agccactaaa cgataccgaa aggatgctgg cgctggcgga
    14401 aaatatgctc gaccgctacg gcatcatcag tcgtcaggcg gtgatagccg aaaatatccc
    14461 tggcgggttt ccatcgatgc aaacactttg tcgaagtatg gaagactccg ggcgaattat
    14521 gcgaggtcgt tttgtagaag gtctgggtgg cgcgcaattc gctgaacgtc tgactattga
    14581 ccgattgcgc gatctggcga cacaagccac gcaaacacgc cactatacac cagtggcgct
    14641 ctccgccaac gatccggcta atgtgtgggg aaatcttctg ccctgggctg cacatccggc
    14701 aacgctggta ccaacgcgtc gggcgggtgc gctggtggtc gtttctggcg gcaaattgtt
    14761 actctatctg gcgcaaggcg gcaaaaaaat gctggtctgg caggaaaagg aggaattact
    14821 cgccccagag attttccagg cgctgaccac cgcactgcgt cgcgaaccaa ggctgcgctt
    14881 tacgctaaca gaagtgaatg atcttccggt ccggcaaacg ccgatgttta cgctgctgcg
    14941 tgaggcggga ttttcaagtt cgccacaagg gctggattgg ggatagttga catcctccac
    15001 gccctgaaag acgtggttcc ctgctacgtt caagctgtcg cctgaatcat ttcggtgggt
    15061 tcctgcttca acgagcggcc tgactgcacc atctctccac aggcaagaac ggcgtgcccc
    15121 gccgctaaaa tgttacgagc gccatttaca tcggcgttcg ctgtatatcc acatacctgg
    15181 catctgaatt tactttgtga caggcggttt tctttcgctg tatgaccaca gcacgcgcaa
    15241 cgctggcttg tgtacgctgg cggaacagca agcacctgac cgccactcca gagctgcttg
    15301 tactcaagct ggcggcgcat ttcataccag ccctgatcca gtatcgaacg gtttaaacct
    15361 gattttgccc ggacattgcg ccccggctgg ctgaccgtac ccgctgctga ctttgacatg
    15421 ttgctgacct tcaaatcctc aatgactatc attgcgtggt ttttgctgac ggtcgttgtg
    15481 actttgtgaa ggtagtccct gcggatattt gcgatacagg aatgcagtcg ctgtattttg
    15541 cgtttctgct tctgccagtt gttgctgaat ttgaccttcc ggcttaactg tcgctgaagt
    15601 ctcgccagcg tcttctggtt tttttgaaaa ctgtttacag gctcaaagac tgtgccatct
    15661 gacagcgtgg cgagtttagc cacgccagca tccagcccga tcattgatgc tgaagggtga
    15721 accggagttg atacttcact ttctgtctga atactgatgt accacgtacc gcaggactgg
    15781 ctgacagtga catttttcac aactcccgtg acctgacggc tattccggta gcgcatccag
    15841 ccaagtttcg gcagaaaaat acggctgttt tcctgatcga gcttaacgtc ctgcgggtag
    15901 cggaatgcag cattctgtcc ccgctttttg aatcggggaa aagcagcccg cttctggaag
    15961 aagtttttgt aagcccgctc aaggtctttc agtgactgtt gcaatggctg tgagggcgaa
    16021 tctttaagcc attgcgtttc agtggcgttt ttccactcaa ccagccagga agccattttc
    16081 ccgtaaggga tgtatttatt accggcctca tgattctcat tctgaagcgc cagagcacga
    16141 ttgaaaacga aacgacatgc gcctgcgaag cgcctcattt cacgctcctg ttgaccaccg
    16201 ggtcttaact ggaatttaaa tgcctgtaat cgtttcacag gtcaaatgat actttggtct
    16261 gtgaaaaaag aaaccgatat ccggcgtgga aggcattgtg ttttcctgat gcatgttcac
    16321 ctggtctttg tcaccaaata ccgacgccag atttttgacc atgacacgac agaaaaacta
    16381 cgcacttact tttcaaatgt atgtgctgat tttgcagctg aactggttga aatggatggc
    16441 gaaccagatc acgtccattt gttaattaac tatcctccca aactggcgat atccagtctg
    16501 gtaaacagcc tcaaaggcat atcgaatagg ttactgcgac gagatcgacc agatattgca
    16561 gtcaggtatt actacaaagg cgttttgtgg agtcctggct attttgccag tagctgcgaa
    16621 ggtgcgccaa tatccgtcat ccgccagtac attgaacaac agcaaacacc tggtcaggtg
    16681 gaaaaccgcg ccttatatcc ccgccctgaa ggacgaggtt ttacggcgca ccggataaag
    16741 gactgacgga tgcccgttcg catccgtcag tattgcagga cggattattc cgcgtccggc
    16801 tcttcagact tgtatttagc ggcagtttct ttgatcagct gctgcagttc gccacgctga
    16861 tacatttcga tcacgatatc acaaccgccg accagctcgc cgtcaaccca cagttgcggg
    16921 aaggtcggcc agttagcata tttcggcagt tccgcacgaa tgtccggatt ctgcagaata
    16981 tcaacatagg caaaacgttc gccacatgcg gcaagcgcct ggactgcctg ggcagagaaa
    17041 ccgcagctcg gcagtttcgg tgaacctttc atgtacagca ggatcgggtt ttcagcaatc
    17101 tggcgttgga ttttttcgat agtggtgctc attgtcttgc ttcctcaaac ttctttacgg
    17161 cagtaatctg acattgtagc gggtcagtgc ggcatcggaa aataacattt tcatcacgct
    17221 tttgctattt tatccctttg ctctatttgt tgcatttcaa atattcgttt cttctctgat
    17281 gcgcggcaaa ctggcgtatt tataaccatt tttagctttc actgctgcat ttttttgcgc
    17341 tcgccaacga aacgtatttt ttaacaataa aagctattaa ctttctcttc ttctatgcat
    17401 tagaatcatc aagttttgta aatcagacgc aggcatgata gacctgcctt aacagaggga
    17461 cgctcagtgg cgcggataaa ccgtatttcg atcacgctct gtgctttact ttttaccacc
    17521 ctgcctttaa cgcctatggc ccatgcttca aagcaagcca gggagagttc tgctaccact
    17581 catatcacca aaaaagcaga taaaaagaaa agcacggcaa ccaccaaaaa gacccagaaa
    17641 gccagccaga aaacagcgaa aaaagccgcc agtaaaagta tgaccaaaag caaaaccgct
    17701 tcttctgtta aaaaatcttc cattaccgct tctaaaaacg ccaaaactcg cagcaaacac
    17761 gccgtcaata aaacggcctc agccagcttt accgaaaagt gtaccaaacg taagggctat
    17821 aaatcgcatt gtgtgaaagt caaaaatgcc gcgtcaggaa cccttgccga cgcgcacaaa
    17881 gcgaaggtgc aaaaagccac gaaagtggca atgaataaac tgatgcagca aattggtaag
    17941 ccatatcgtt ggggcggcag ctcaccgcgt accggttttg attgcagcgg cctggtttat
    18001 tacgcttata aagatttggt gaaaattcgt attccgcgta cggcgaatga aatgtatcac
    18061 ctgcgtgatg caggacctat cgaacgtagt gagctgaaaa acggcgacct ggtctttttc
    18121 cgtactcagg gacgcggtac agccgatcat gtcggcgtgt atgtcggcaa cggcaaattt
    18181 attcagtcac cgcgcacagg tcaggaaatt caaatcactt ctctcagtga agactactgg
    18241 cagcgccact atgttggcgc tcgtcgggta atgaccccaa aaacacttcg ctaaaacttt
    18301 accctgttgt tacggcaaca gggtaagttc atcttttgtc tcacctttta atttgctacc
    18361 ctatccatac gcacaataag gctattgtac gtatgcaaat taataataaa ggagagtagc
    18421 aatgtcattc gaattacctg cactaccata tgctaaagat gctctggcac cgcacatttc
    18481 tgcggaaact atcgagtatc actacggcaa gcatcatcag acttatgtca ctaacctgaa
    18541 caacctgatt aaaggtaccg cgtttgaagg taaatcactg gaagagatta ttcgcagctc
    18601 tgaaggtggc gtattcaaca acgcagctca ggtctggaac catactttct actggaactg
    18661 cctggcaccg aacgccggtg gcgaaccgac tggaaaagtc gctgaagcta tcgccgcatc
    18721 ttttggcagc tttgccgatt tcaaagcgca gtttactgat gcagcgatca aaaactttgg
    18781 ttctggctgg acctggctgg tgaaaaacag cgatggcaaa ctggctatcg tttcaacctc
    18841 taacgcgggt actccgctga ccaccgatgc gactccgctg ctgaccgttg atgtctggga
    18901 acacgcttat tacatcgact atcgcaatgc acgtcctggc tatctggagc acttctgggc
    18961 gctggtgaac tgggaattcg tagcgaaaaa tctcgctgca taataactga tggcaaatgc
    19021 agcattgcct gatgcgcttc gcttaccatg ccaacgcgga tgaggcgtta acagcgcatt
    19081 cggcaagata aatcgcacgt tgtcagcaac tgtaacgcag aaggttatcc ttctgcgttt
    19141 ttgtttaatt agctgttagc aacgcaaact gtttcaggtt gttttctggc tgacataaac
    19201 accagcaata atgccagtcc cgcgacaatc gcccccgtca ccggcacaaa gctgtatccc
    19261 agcccagcgg aaattaccgc accaccagca gctgctccca gcgcatttcc aagattaaag
    19321 gcaccaatat tgactgatga agacagacct ggcgcttcac tggcgacatg catcacgcgc
    19381 atctgtaacg gaggtacaac cgcaaaggtt gctgcgcccc acaccaccat gctaatagct
    19441 gcgccgaact cattgcgggc caggaacggg attgccagca taatcaccat cagcagcaac
    19501 aaaaagcctt tcaacgtgcc gttaactgaa cgatctgcca gtttgccgcc gagatagtta
    19561 ccgatagaga atccgacacc aatcagcacc agcattgccg tgacgaacac cggtgttgcg
    19621 tgggtaatac tttgcagtac cggagagata taggtgtaga gggtaaacat tgcaccagct
    19681 cccagtaccg tcgtcagcaa tgcagacagc acctgcggac gcattaatac cgccagctct
    19741 tttttcactt caggtcgtgc ccctgtacca cctttaggta atgagaagaa cagacttacc
    19801 attgaaatca ctcccagccc cgccgttgcc agaaatgaca tccgccagcc gatggtttca
    19861 cccaaccagg tcgccgccgg cacgccaccg atatttgcca gggttaaccc cataaacata
    19921 gtggcaactg cgctggcctg tttatgtttt ggcaccacgc ttgcggccac gactgaaccc
    19981 aaaccaaaaa atgctccgtg attcaggctg gtcaaaatac gtgaaagcat cagggtcata
    20041 taatccggcg cgatggcgga aagaacgttg cctagcgtga aaattgccat caggaaaatc
    20101 aacgcactgc ggcgggcacg atgagaaagt agaagcgtca tcagcggcgc gcccaccatt
    20161 acgccaactg cataggcact gattaacatt ccggcagcgg gaatcgagac atccacaccg
    20221 cgcgcaatga cgggcaacaa gcccattggc gagaactccg ttgtcccgat accaaacgcg
    20281 ccaatcgcca gcgccagcaa cggatagtta attttcatgc cttatctcca cctcttcgcg
    20341 tcattacgcg atattcatta aagtggcgaa agcatgacag caatcacaaa aaaatgaaaa
    20401 taacaaaaag agaaaacact tttgccattt tgctaacaaa caggaaggag atgcgaggga
    20461 gaacgcgctc cctcgagagg aaatcagtgc agcgcggcag tcaaacccac ggctacgatc
    20521 aaaccgagga cgataaccgt tgttaccagt gaaaatttaa ggtcggtgct catcaagttt
    20581 tctccttttt tattaccaca caaaaagtga tattacgcat ttttacacac tgtgatgaaa
    20641 aaatctcccg tcatttataa tgataagtgt ttttaccact tccccttttc gtcaagatcg
    20701 gccaaaattc cacgcttaca ctatttgcgt actggccatt gaccccttcc tgacgctccg
    20761 tgtcgttttt ccggcgtacc gcaacacttt tgttgtgcgt aaggtgtgta aaggcaaacg
    20821 tttaccttgc gattttgcag gagctgaagt tagggtctgg agtgaaatgg aatggcaaca
    20881 ataaaagatg tagcgaaacg agcaaacgtt tccactacaa ctgtgtcaca cgtgatcaac
    20941 aaaacacgtt tcgtcgctga agaaacgcgc aacgccgtgt gggcagcgat taaagaatta
    21001 cactactccc ctagcgcggt ggcgcgtagc ctgaaggtta accacaccaa gtctatcggt
    21061 ttgctggcga ccagcagcga agcggcctat tttgccgaga tcattgaagc agttgaaaaa
    21121 aattgcttcc agaaaggtta caccctgatt ctgggtaatg cgtggaacaa tcttgagaaa
    21181 cagcgggctt atctgtcgat gatggcgcaa aaacgcgtcg atggtctgct ggtgatgtgt
    21241 tctgagtacc cagagccgtt gctggcgatg ctggaagagt atcgccatat cccaatggtg
    21301 gtgatggact ggggtgaagc aaaagctgac ttcaccgatg cggtcattga taacgcgttc
    21361 gaaggcggct acatggccgg gcgttatctg attgaacgcg gtcaccgcga aatcggcgtc
    21421 atccccggcc cgctggaacg taacaccggc gcaggccgcc ttgccggttt tatgaaggcg
    21481 atggaagaag cgatgatcaa ggtgccggaa agctggattg tgcagggtga ctttgaacct
    21541 gaatccggtt atcgcgccat gcagcaaatc ctgtcgcagc cgcatcgccc tactgccgtc
    21601 ttctgtggtg gcgatatcat ggcaatgggc gcactttgtg ctgctgatga aatgggcctg
    21661 cgcgtcccgc aggatgtttc gctgatcggt tatgataacg tgcgcaacgc gcgctatttt
    21721 acgccggcgc tgaccacgat ccatcagcca aaagattcgc tgggtgaaac agcgttcaac
    21781 atgctgttgg atcgtatcgt caacaaacgt gaagaaccgc agtccattga agtgcatccg
    21841 cgtttgattg aacgccgctc cgtggctgac ggcccgttcc gcgactatcg tcgttaatca
    21901 cccgttgcgg gagtctcttc cggctcccgc agccactcct tattcagcgt ctcactatcg
    21961 ctgagatact caagcagcca ggttaacgca ggcgacatat cattttgctg ccatgtcaga
    22021 caacatgccg aatccgggaa ggggttttcc agttcaagtg ctacccactt cccctcatta
    22081 agccacggtt tggcgaaatg tgttggcacc atccctatgc ataatcctgc cgagatacag
    22141 gttgccgatg attcccagtc aggtacgaca agtctttttt ggttatccag caaccaggta
    22201 atacgtttag gtagcgttcg cgaggtgtct tcgcgcacca acgacggcca gttgcgcaac
    22261 gtatcatcgc tgaacgggcc atccatcaac gccagcgggt ggtgactggc aacaacgcaa
    22321 ctccagctta gcatccccat atcccggaag gcataacgac cgcctaccgg aatcgcgcgt
    22381 gttgcgccaa ttgccagttc cacgcgcccg tcggaaagcg catcccagac accgttgaac
    22441 acttcctgaa agacaagaag ttcgacatca tcaaaatggc gataaaaatc aacgatcatc
    22501 tgccgtgtac gttctggcct gacaatatta tccactgcga tagctaactg accgcgccag
    22561 ccgttcgcta tctgctgaca ttgctggcgg gtgatctgca tttttttgac aacagagcgc
    22621 ccttctttga gaaaccacgc tccagcagcg gtcagctcca catcacggtg ccgtcgttca
    22681 aagagcggta ccgccagcca ctcttccagc tgacgcacgg tatagctgac cgcagaagga
    22741 acacgatgca gctcctgtgc cgcagcgcta aaactaccat tacgcgctac cgcatcaaca
    22801 acttcgagtg aatattctga ccacatagtc tgcctgcaaa atttttgaaa ccagtcatca
    22861 aatattaccg tttcacaaca ctaatttcac tccctacact ttgcggcggt gtttaattga
    22921 gagatttaga gaatatacat gcaacctggg aaaagatttt tagtctggct ggcgggtttg
    22981 agcgtactcg gttttctggc aaccgatatg tatctgcctg ctttcgccgc catacaggcc
    23041 gacctgcaaa cgcctgcgtc tgctgtcagt gccagcctta gtctgttcct tgccggtttt
    23101 gccgcagccc agcttctgtg ggggccactc tccgaccgtt atggtcgtaa accggtgtta
    23161 ttaatcggcc tgacaatttt tgcgttaggt agtctgggga tgctgtgggt agaaaacgcc
    23221 gccacgctgc tggtattgcg ttttgtacag gctgtggggg tctgcgccgc ggcggttatc
    23281 tggcaagcat tagtgacaga ttattatcct tcacagaaag tcaaccgtat ttttgcgacc
    23341 atcatgccgc tggtgggtct gtctccggca ctggctcccc tgttaggaag ctggctgctg
    23401 gtccattttt cctggcaggc gattttcgcc accctgtttg ccattaccgt ggtgctgatt
    23461 ctgcctattt tctggctcaa acccacgacg aaggcccgta acaatagtca ggatggtctg
    23521 acctttaccg acctgctacg ttctaaaacc tatcgcggca acgtgctgat atacgcagcc
    23581 tgttcagcca gtttttttgc atggctgacc ggttcaccgt tcatccttag tgaaatgggc
    23641 tacagcccgg cagttattgg tttaagttat gtcccgcaaa ctatcgcgtt tctgattggt
    23701 ggttatggct gtcgcgccgc actgcagaaa tggcaaggca agcagttatt accgtggttg
    23761 ctggtgctgt ttgctgtcag cgtcattgcg acctgggctg cgggcttcat tagccatgtg
    23821 tcgctggtcg aaatcctgat cccattctgt gtgatggcga ttgccaatgg cgcgatctac
    23881 cctattgttg tcgcccaggc gctgcgtccc ttcccacatg caaccggtcg cgccgcagcg
    23941 ttgcagaaca ctctgcaact gggtctgtgc ttcctcgcaa gtctggtagt ttcctggctt
    24001 attagtatca gcacgccatt gctcaccacc accagcgtga tgttatcaac agtagtgctg
    24061 gtcgcgctgg gttacatgat gcaacgttgt gaagaagttg actgcccgaa tcatggcaat
    24121 gccgaagtcg ctcatagcga atcacactga tctatatcga tatacttata cttaggctgc
    24181 taacaaaatt ttgttgtatg attgaaatta gcggcctata ctaatttcga gttgttaaag
    24241 ctacgataaa tattatgttt ttacggggac aggatcgttc ccgactcact atggatggtc
    24301 atttcggcaa gggttcctcc tttccctctg ttctacgtcg gattatagac tcgcggtttt
    24361 ttctgcgaga tttctcacaa agcccaaaaa gcgtctacgc tgttttaagg ttctgatcac
    24421 cgaccagtga tggagaaact atgagttcat cgtgtataga agaagtcagt gtaccggatg
    24481 acaactggta ccgtatcgcc aacgaattac ttagccgtgc cggtatagcc attaacggtt
    24541 ctgccccggc ggatattcgt gtgaaaaacc ccgatttttt taaacgcgtt ctgcaggaag
    24601 gctctttggg gttaggcgaa agttatatgg atggctggtg ggaatgtgac cgactggata
    24661 tgttttttag caaagtctta cgcgcaggtc tcgagaacca actcccccat catttcaaag
    24721 acacgctgcg tattgccggc gctcgtctct tcaatctgca gagtaaaaaa cgtgcctgga
    24781 tagtcggcaa ggagcattac gatttgggta atgacttgtt cagccgcatg cttgatccct
    24841 tcatgcaata ttcctgcgct tactggaaag atgccgataa tctggaatct gcccagcagg
    24901 cgaagctcaa aatgatttgt gaaaaattgc agttaaaacc agggatgcgc gtactggata
    24961 ttggctgcgg ctggggcgga ctagcgcact acatggcatc taactatgac gtaagcgtgg
    25021 tcggcgtcac catttctgcc gaacagcaaa aaatggctca ggaacgctgt aaaggcctgg
    25081 atgtcaccat tttgctgcaa gattatcgtg acctgaacga ccagtttgat cgtattgttt
    25141 ctgtggggat gttcgagcac gtcggaccga aaaattacga tacctatttt gcggtggtgg
    25201 atcgtaattt gaaaccggaa ggtatattcc tgctccatac tatcggttcg aaaaaaaccg
    25261 atctgaatgt tgatccctgg attaataaat atatttttcc gaacggttgc ctgccctctg
    25321 tacgccagat tgctcaatcc agcgaacccc actttgtgat ggaagactgg cataacttcg
    25381 gtgctgatta cgatactacg ttgatggcgt ggtatgaacg attcctcgcc gcatggccag
    25441 aaattgcgga taactacagt gaacgcttta aacggatgtt tacctattat ctgaatgcct
    25501 gcgcaggtgc tttccgcgcc cgtgatattc agctctggca ggtggtgttc tcacgcggtg
    25561 ttgaaaacgg ccttcgagtc gctcgctaaa ggctattcta tcgccccctc ttcgggggcg
    25621 agttcagatc aggcttctgt acctggttga ttcatggcat tttctcgtgc cgccagcaca
    25681 cgttctaccg tatctaccac tgcctgagtt tgtggatcga tttcaatgtt gacgcgtgcg
    25741 ccaagttttt tcttcccaag agtcgtgcgt tccagtgttt ccggaattaa atggacgcaa
    25801 aaacgcgttg gcgtgacttc gccgacggtc aggctaatac cgtcgatgcc aataaatcct
    25861 ttgtacagga tatatttcat caactgacta tcctggactt taaaccagat ctggcgatta
    25921 ttttccgagg ttaatatttt cgccacttca gcggtggtca taatatgacc tgacattaag
    25981 tgtccgccaa tttcatcact gaatttcgcc gcacgctcaa cgtttaccca atcccccact
    26041 tttaaatcgc caagattggt aatgcgtaac gtttctttca tcaggtcaaa actgacatgg
    26101 ttgccgttaa tttccgtcac ggtcaggcag caaccgttat gcgccacgga agcaccggtt
    26161 tccaggccgt ccagcatgtg gtcgggtaac tccaccacat gcgtacgaaa atttggtttc
    26221 tcgtcaatcg acaccagttt tgcggtgccc tgtacaatcc ccgtaaacat acttacaact
    26281 cctgaaatca gttaagacat tctgttcagc acaatagcag gtggaaaacg cccttaccag
    26341 tgaaggggta agaatggcta ttttttcact ggagaattaa taaatcctcg ctacaataga
    26401 ctgaatttcc cctgcttctt ctttttgctg cccattcagg cggcttttta gtctctcata
    26461 taactacaaa taaaaggtgt tcacgtgcag aagtatatca gtgaagcgcg tctgttatta
    26521 gcattagcaa tcccggtgat tctcgcgcaa atcgcccaaa ctgcgatggg ttttgtcgat
    26581 accgtgatgg cgggcggcta tagtgccacc gacatggcgg cggtcgctat cggtacttct
    26641 atctggcttc cggcgatcct ctttggtcac ggactgctgc tggcattaac gccggttatc
    26701 gcgcaattaa atggttccgg tcgacgtgag cgcattgcgc atcaggtgcg acaaggtttc
    26761 tggctggcag gttttgtttc cgttctcatt atgctggtgc tgtggaatgc aggttacatt
    26821 atccgctaca tggaaaacat cgatccggct ctggcggaca aagccgtggg ttatctgcgt
    26881 gcgttgttgt ggggcgcgcc gggatatctg ttcttccagg ttgcccgtaa ccaatgtgaa
    26941 ggtctggcaa aaaccaagcc gggtatggta atgggcttta tcggcctgct ggtgaacatc
    27001 ccggtgaact atatctttat ttatggtcat ttcggtatgc ctgagctcgg tggcgttggt
    27061 tgtggcgtgg ctactgcggc ggtgtattgg gtcatgttcc ttgccatggt ttcttacatt
    27121 aaacgcgccc gctccatgcg cgatattcgt aacgaaaaag gcaccgctaa gcccgagcct
    27181 gcggttatga aacgactgat tcaactcggt ttgccgattg cgctggcact gttctttgaa
    27241 gtgacactgt ttgccgtcgt ggctctgtta gtgtctccgc tcggtattgt tgatgtcgca
    27301 ggacaccaga ttgccctgaa ctttagttca ctgatgtttg tgcttccaat gtcgctggcg
    27361 gcagcggtaa ctatccgcgt aggttatcgt ctgggtcagg gctcaacgct ggatgcgcaa
    27421 accgctgcgc ggaccgggct tatggtgggt gtctgtatgg caaccctgac ggccattttc
    27481 acggtttcac tgcgggagca aatcgccctg ttgtacaacg acaatcccga ggttgtaacg
    27541 ctggctgcgc atttgatgtt gctggcggcg gtatatcaga tttctgactc aatccaggtg
    27601 attggcagtg ggattttgcg tggttataaa gatacgcgtt ccattttcta tatcaccttt
    27661 acagcttact gggtgctggg cttgccaagc ggctatattc tggcactgac cgatctggtc
    27721 gttgaaccta tggggccagc aggcttctgg ataggcttta ttatcggcct gacatcggca
    27781 gccattatga tgatgctgcg tatgcggttc ctgcaacgta tgccgtcagc catcattctg
    27841 caacgagcgt cccgctaata aagacaaggc gcaaccttca cgggttgcgc ctgtgttttt
    27901 acgcaggctg gagcgttgcg ccaatcccgt cttcgtctgg ctgtaatttc agagcgttac
    27961 acagagtttg ccgactggca tctgttatct caacgcgtat atccccgtta acttcccctt
    28021 tcacattgat gaagtcaggt gaaatttctt tcgcactggt ttgcaggcaa atcgctccct
    28081 ctccgcgcag gcgtttcact ttcagttgca catgcgttgc cgcaccagtg atcagccaca
    28141 gttttccgcc attcagattg atgttaccaa caatgaaagt gacgctgtca ccttttaccg
    28201 tactgccgtc gagaatcaac acaccgccct gctgtacagt aatcgttgat tgttgcagct
    28261 ctgcgccata accaataatc attgaagcgc cgtccagtaa tgcaacactc ccggtcagtt
    28321 caaccggtgc gcgatcttcc tctacgacaa aattttcgtc cgcgctggtg ggtgacaaca
    28381 ggatcactgt tccaccttgt attaccgcat tttccagcgt accggcatgc acttcagccc
    28441 gcccccccac attcaccgac aggttttgcg tcttaccgga actgtagagc tgaaggccat
    28501 ccgtccccag acgataaatg gatccattct caacaatagt gtcagagtct tcaccaccga
    28561 gattattcat ggtgccacca gcaagcaacg tatcactggc tttcccggcc agcataaaaa
    28621 cgccgccctg attaatctca caattgctgg cttctccact tggatacacc aacaattcac
    28681 caccttcatc taccacgacc gctgaggttg tcccattaac caacaggccg ccctcttgat
    28741 cgagaatgat tttttccgcg cgatgtcctt ccagtacacg caggttaccg ccattttcca
    28801 gcaacaaacc gcaggcataa ccctgatcaa cgctgaattc accttcggga tggcggccat
    28861 taaccgtagc gagcgtagag agactaattg cgcctccctc ggactgaaca atatctgtgg
    28921 caatggcacc gtcgctgacc gatagcgtgc cattgttacc gtactgtgtg catttggcga
    28981 tcccaccggt tgccactttc aaccagccag cccccgtgac cacggtattt tcggctcttc
    29041 cgccagcttc aacaatcaac tcgccttcag taagttgcgt cgcgccaagg acctcgccgg
    29101 tcttaacaat ttgcacattc ccgtcgctca tcaagttttt cgcatcagat cccatgattt
    29161 attcctttgc tgcatctgtg tgcctttatt gctacctaag tgtaaaggct acggaggatt
    29221 tatccacgac agatttgaga tggtggcaaa caactctgtt taaactctga tacacgaatt
    29281 attgggttgt atcagatgta aatgcgatcc tgaataaaaa tcgcccttgc aaatcaacaa
    29341 aatatagcca atttgcttaa aagctctgca aacggtcgaa atagcgcaga aaattacgtt
    29401 ttgcctcttg ccacctttcc actctgccgc taatattcgt ccccgttgtc acctacaacg
    29461 ttgcgttcat agctcagttg gttagagcac caccttgaca tggtgggggt cgttggttcg
    29521 agtccaattg aacgcaccat cctgcgtccg tagctcagtt ggttagagca ccaccttgac
    29581 atggtggggg tcggtggttc gagtccactc ggacgcacca gattttctta atctggtctt
    29641 ttcctttttc cctctgtttc ttctctgtat ccaatacgtt aaaagattta cactgtcttc
    29701 gtatgcgtta tcagaaggag aatcgctatg gcaactttgt tacaacttca ttttgctttt
    29761 aatggcccat ttggcgacgc aatggctgag cagcttaaac cacttgctga gtcgattaat
    29821 caggaacctg gttttctgtg gaaggtatgg acagaaagtg aaaagaacca cgaagccggt
    29881 gggatctacc ttttcactga tgaaaaaagc gcccttgcct atctggaaaa acatactgcc
    29941 cgactgaaaa atctcggcgt tgaggaagtt gtcgccaaag ttttcgatgt caatgagcca
    30001 cttagtcaaa tcaatcaggc aaaactcgcc tgacagaatt taatcaaggg cggttagcgc
    30061 ccttttcatc cctgtctgaa atttctcaaa ttctaaaaat ctcaaccaaa cttatctgat
    30121 aacactaaat tcgaaagaat gcgtacaggt aagtaacaat gaaaaaaatt gctattgtgg
    30181 gtgccgggcc tacggggatc tacaccttat tctcgcttct acagcaacaa actccacttt
    30241 ctatttctat cttcgagcag gctgacgagg ccggtgtcgg gatgccatac agtgatgagg
    30301 aaaactcaaa aatgatgctg gcaaatattg ccagtattga aataccgccg attaattgta
    30361 cgtatctcga atggctacaa aagcaagaag ccagccatct ccagcgttat ggcgttaaaa
    30421 aagaaacctt gcacgatcgt cagtttttac cgcgaattct gctgggcgaa tatttccgcg
    30481 atcaattttt acgattagta gaccaggcac gacagcaaaa atttgcagtg gctgtttatg
    30541 aatcatgcca ggttaccgat ctgcaaatta caaatgctgg cgtcatgctc gctacaaatc
    30601 aggatttacc cagcgagacg tttgatttag cggtgattgc cacgggtcac gtctggcctg
    30661 atgaagaaga agcaaccaga acgtattttc ccagcccgtg gtcaggcctg atggaagcaa
    30721 aggtcgatgc gtgtaacgtg ggtattatgg gaacatcctt gagcggactg gatgcggcaa
    30781 tggcagtggc tattcagcat ggttcgttca ttgaagatga taaacaacac gtcgtttttc
    30841 accgcgataa cgcaagtgaa aagctaaata ttacgttgat gtcgcgcacg ggtattttac
    30901 ccgaagccga tttctattgc cctattccct acgagccctt acacatcgtc accgatcagg
    30961 cattaaatgc tgagattcaa aaaggtgaag agggcctttt ggatcgggta tttagattga
    31021 tagtagagga aatcaagttt gctgatccag actggagcca acgcatagcc ttagagagcc
    31081 tgaatgtcga ttcctttgct caagcctggt ttgccgagcg caaacaacgc gacccatttg
    31141 actgggcaga aaaaaatctc caggaagtcg aacgcaataa acgagaaaaa catactgttc
    31201 cctggcgtta tgtcattctg cgcctgcatg aagccgtaca ggaaattgtt cctcatctga
    31261 atgaacacga ccataaacgg ttcagtaaag gccttgccag ggttttcatc gataattatg
    31321 cggcaatccc ttcagagtct attcgtcgac tactggcctt acgtgaagcg gggatctgaa
    31381 atccctgatg ttggcgaaga ttatacgtta cagcaacccg aagatattcg tggacgcgta
    31441 gcgttcggcg cgttgccctg gttgatgcac gaccagcctt tcgttcaggg acttacggca
    31501 tgtgcagaaa ttggtgaggc gatggctcgg gcggtcgtaa agcctgcatc ccgtgctcgt
    31561 cggcgtcttt cgtttgatta aaggtaaagc tatcagcact caacaaactg atgctgttca
    31621 ttaagacgat agcggactcc ggttcgaata ttgttttcac cttcaatggc gacggcgaag
    31681 cgaactcttt cgccgtcatg atatgccagt gcggcgcttc cccctagccc gagaataatt
    31741 gagtcgacca cgcccgtgct cactaccgtt gaattttcac cgctggcaat gatgtgtaca
    31801 ttgtcgccgc tgttggcaat tctggcgttt gcgccaaagc tggcgatttg caccaggtca
    31861 ccattactgg cgacatgaca ccgttcaccc aatgtacaaa cgcgtactcg cattccggta
    31921 ttcgcaatcc gactactatc tccagcgctg cttatccgcg aagaattacc ggttacagca
    31981 acccgtgcgc gttcaccgga gctgccaatg tgactgttaa agccgacact tccaatccgc
    32041 acgctataac ccatactggc aatttgcgcg gcatacccag cggttgcgat tcgggcgtta
    32101 tcctcacaac agccgatgcg agcatgatcg tcgctgcggg caaactgact gcaaaatggt
    32161 atctctccgg ggtctgtggc cagtttttgc atagaggaaa cttcctgatg cgcgaagttt
    32221 tcatccgcca gccatttttt ccacgcatac tcaaccagac tctccgccca gtccgtatat
    32281 ccttcctcaa tcagtgcatg acgaatatcg gcataactac cgccgcgtgg gaaatggcgc
    32341 aggaaccagc gatacatcac cgctccgata cgccattctc gcaggtctgc tcgtgtaatg
    32401 atcattcgtt agtgccggtg atagccatcg accatgcttt taaaggtttc gtgtggcgta
    32461 cgccccgtgg tgcaaagata aagatgaccg aagataaaaa agaggcttat aaatgccaga
    32521 gcaaaatgcg cctgcaataa ccagtatctt acgccaggaa acacatctcc cacggcttgc
    32581 ggatagagac acaacagccc cgtcaatagt aacaacggca gcaatccata catgacacca
    32641 acataggcga cctgctgtaa gggattaaat ttagactgtg ttgttgccgg gaaaggatgt
    32701 tcttccccct gcataatgcc aaacaaataa aatcgcgttt gttttgccgc tcgttccagc
    32761 cacccctgac gacgaatgcg atagtggtga ccattatccc caacggcatt gatcagcaca
    32821 aagccgagcc agcatgccag taacaaaaat ccgcaaactt catgcaccgc aaccagactt
    32881 ttaaccgcag ttgcgcccac catcgcaaag tgatttatca gcccacttgc cagcaacaat
    32941 acaaagagta acgcattcga ccagtgccat aatctgaccg ctttgctgta aagataaacc
    33001 ttctcaccat gatcggtggc tgatttcttc acgccacgag cacgaagcaa ggcgtgcagc
    33061 cccagcacca accacatccc aaccagcaat actccggcaa tgatcaacca caccggccaa
    33121 aattcgggag tgaactgtgg cacatagttc gctaactggc tctgaaactg ttcagcatgt
    33181 tgcgacgggt tcatacattt tcctttttaa tcaaatgttg accgaaccgg cgatacagat
    33241 gcggttttcc ggcaccaggt agctgatatt gatagtattt attatcctgc aaccacgcct
    33301 gaatttctgg gctatcttcg cgaccaaaga tcaatgcgtg ttccggacag gcgctgacgc
    33361 aaatgggcgg aaagcctttt gccagtcggg actcagcgca gaaatcacat ttatctgcca
    33421 ctttagtgac agggttaagg tagcgaacca ggtacggaca agcgccaatg caataactgc
    33481 agccgatgca ctgcgatttt tctacccgca cgatcccctg ctcatcacgc cacgatgccc
    33541 ccgtcgggca tacgtcgatg cacggagcat cttcgcagtg ctgacatgac tggcgaaaaa
    33601 agtgatactg cgtttcgttg tcgttatcgg tgactggaat gtgtgctatc gataaacggc
    33661 ttccctgagc ggggacgtgg ttagttttac ggcatgcgcg ggcgcaaata ttacagccat
    33721 tgcaccgtga ctcgtcatga atcatggcat accgaacttc cttttcttgc ctcgtgttcg
    33781 ctaacagaga agacgcagaa ccagtaaaaa agattactgt tcccatcccc agaacaaatt
    33841 tgcgtcgagt gaaggacatc tgattatccc ctaatgcagt ggctgcaact gtccacccta
    33901 tccatgacat ctccctgaaa tttcaggggt tctgaaactg ctctacctcg aaccattcgc
    33961 ataagccata agccttgcca taggtcataa atcgctcgct aaacaagtac aacaccgccg
    34021 tcgatgactc cacctgccgt atatcggcat attcaggtgc aacatccatc gcggcgatcg
    34081 cggcttcaat ctgcgcttcc tggaagtaat atggcgcttg catcagcatg gcgactttgt
    34141 aaggacgagg atacgtctgg cactcaaaac gaacggcgtg tgcaatggcg cgacaaatgt
    34201 cctgctccac aacttgcaaa gacatcgccg cgtagttttc actcatggcc tgcgttgagt
    34261 aatagtaatc atcctgactt cccgtcatac aagcgatatc cgcgtagtct ccgttctgct
    34321 taatgctttc cagcaaaact gacagttcct cttctgcaac cgaatagggt ggcgagagaa
    34381 acacggtgcg cgccaccagc tgcccagccg cagaatgctg gcgaatgaac tcagccagga
    34441 tttcagcagg ttctggttcg gttaccgtat caacagggtg acgcatcccc tgcaaccatt
    34501 gccgcttcgc ctcgtcaact tcgctgactt tcgctaacgg caattcatcc tgatggttca
    34561 tcttacccat tccttacgca ggcagtagat tgtgtgctgc cagatcggcg gcaatatctt
    34621 ccagccccag acgctgcaat gtttcgcggg ttggacaacc aagctgtggg tcccagccca
    34681 tttctttgta gaacatcgtg agcgaagcat gcatatcgtc acgatccatt ttgtcagtac
    34741 cttcagtaaa gaccgggatc tgcggatcct tgtcgaaaac ccaggaacag ataagatcgt
    34801 gttcattacg catatctttg gtttgcatcg gttttaccgt gtaggcacga tgcaacgtaa
    34861 agatacgctc tgcagctaaa tctaattttt cctgagtcat ctcttcgccg gtgatcgctt
    34921 tgaagaattt ggcttcaagc gccagatcgc cccggtaatt acggcttttc agcggcgaaa
    34981 cggtcattgg ccagacccag ttgcacagag tgacggcgtt atgcaaacag acccgcaaca
    35041 gcgaccactt tgcatattta atttttgcgt cattgattgg cgtgtagttt ttggtttcat
    35101 cgtaagcatc ttcagaacca aaaagttctt tcgccacttc acgttgcagt ttcaatggca
    35161 agccggaacc aataaagttg atatgggtat gcgtcatgca gtcacggttg aacatacact
    35221 taacaatgga accgacctgc gctgacgctt cattggcatg gtgaaccgga tagccaaacg
    35281 gcgaccagag tttatttttc gcgtagcccc agtactcttt acccaaattc cagcgttctg
    35341 cgatggcata tgaaccatca gccaggtgac tcagctcacc cacacgatgc gccagacggt
    35401 agtaaaaatc tttaatgaag ttaacgtcac ccgcttccag ttgatcccag cgaatttctg
    35461 catactcttc agctggcaga acacgcttga acacaccttt gctgtaacaa taagtaaagt
    35521 cgcgatgcaa ctgcccgtag ttacaccata ggccgtagtc atcgaacaaa ttcagaccaa
    35581 ccaggttacc aatcacacga ccatcgtctt tatcttcaaa atctttcggc ccgttcggga
    35641 agatggtggt atggacaaag tttgctacac aagtgttacc acctgtgctg ggcacgccaa
    35701 actctttcac ccgaggaata ttcatttggg tcatacaacg gatcgggcaa gagtggcagc
    35761 cgctcatttt tactgtgtat ttctctgccg ccggtcctaa gtcaaaaacg gatttatagg
    35821 tacgaaagcc gaccgtattc tgattgcctg gcggaatttc acccgtttca atcggaccac
    35881 cttcagccgc gccccaaaac agccctttac gtgctgtcca gcgtgacttg ggatctgcgt
    35941 acttggcacg acatggttgt tattcgcacc aataagttca gtcatcatgt aatcattgag
    36001 acgcttcatc tcctgacgat cggcaatgtt gacccctttc gtcccttcaa ccgcaatcgc
    36061 tttcaggttt ttcgaaccca ttattgcgcc agttcccgca ccgccgctgt ggttacggct
    36121 attcaacatg ccagagagag gaataaggtt ttccccagcc tgaccaatag ccgccacaca
    36181 ggtttccggg ctggtcaatc gacaaatttc ttccgtcgtt gcgcgcgtcc cttttcccca
    36241 taagaaatcg gctttttcca ggctaacttt gtcatcttta atcttcagcc ataccggtga
    36301 tttcgccttc ccttcgataa taatgacgtc gtatccagcg aatttcattt gcgctgcaaa
    36361 aaagccaccc atatgggcat cgcgactaaa tttcctttgg taaaagtaga aagtgaggtg
    36421 atatttacgc gagaactaca gggggcacca gatccaggta atgggccggt agcaaagact
    36481 aatttattcg cttcatcgaa aggtttcgtg cctagcggta cttcgtcata cataattttg
    36541 tagccgaagc ccatgccacc gacaaaactt ttaaacttac tggaatcttc gagggtaata
    36601 tttcctgtcg tgagattgac tcttaatata ttacctgtcc aaccgttagc catgattttt
    36661 tcctttgcaa gattcacaca gtaatatctt tccactcgat aatttttaac gcccctgttg
    36721 ggcaggcgtt tgcgcattca ccgcataaca cacatttcga ggatttttta ctttcggtat
    36781 ttacggtggc catcatccac gggcatgccg tagtacaggc gctacagcca atacaacgtt
    36841 tatgatcgac ggtaatacag ccttctttct gctgccaggt aatcgcacca atcgggcaga
    36901 cgttcatgca ttgcggctct ttgcattgac ggcaggtgtc cgcggtatag ttgagatcgc
    36961 catacaggcc gccgccagag ccaaccccgt tgtcgccaaa gaaataattg cgatggattt
    37021 tgatacggga gaagaatgtc cctactgagc catcgttgaa gttggtacaa gagatttcac
    37081 agcggtggca acccgtgcat cgcgctcgct gggtcaccag cacccctttg ggtgtattta
    37141 tcaaccctac tgtgccgcta tcaatatctt cctgtttgca acccaaaagt gacagtaacg
    37201 caggggcaat tgttagacca gcaaggcctt ttcctgatat acgcaagaat tctaatcgcg
    37261 ttaagccaat atctaatagt ggacgatcaa ccgggttcat ttatttcgca tcctcttcgc
    37321 aaacacagtg ctgacaattc agcagcctga tattaagcat tcaactaata cgctgctgac
    37381 tctgtcccca acaccagata ttttccctaa cccgatagtg tatgagaaaa gatttaacgc
    37441 cgataaacta atcctttatg gttaggagaa aaaataatgt gattattctg agcccttaac
    37501 attgatcgtt atcaattaaa attacaaagc aagaaattat aagcgaactg atatttatta
    37561 tcatttgaaa taaatttaac ttaagcgaca atcatttgtg acgttcattg cagaaaataa
    37621 aaataacatt atcgctataa attaaaatac ataccgaaat tcgtataatt tcagcagttg
    37681 ttatttagaa tgattaactg gaccgcaact gaagcaagaa tatcaggatg aagcttaccg
    37741 cctcatcctg aatggtcatt ttaccagtgc gttaatgacg gtttccatcg cctgcttttc
    37801 cagctcactg cgctgaatgc gtttattggc taatgctgta cgtaatacgc ctgcgataac
    37861 aatatgcttt ggttcctgcc ccagatcgcg catttccagc acaactttac cgacaactct
    37921 gcacatttct cggtacagtt catcctcttt tgtccgattt cccattcttt atacctattt
    37981 atcatgccaa
//
LOCUS       DAJTUI010000030        37628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTUI010000030 DAJTUI010000000
VERSION     DAJTUI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.030"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..176)
                     /locus_tag="OO464_001668"
     CDS             complement(<1..176)
                     /locus_tag="OO464_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR6226850.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="OO464_001669"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="OO464_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6226851.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="OO464_001670"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="OO464_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6226852.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="OO464_001671"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="OO464_001671"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6226853.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="OO464_001672"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="OO464_001672"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6226854.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="OO464_001673"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="OO464_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6226855.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="OO464_001674"
     CDS             complement(5180..5422)
                     /locus_tag="OO464_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6226856.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="OO464_001675"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="OO464_001675"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6226857.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="OO464_001676"
                     /gene_synonym="fabE"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="OO464_001676"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6226858.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="OO464_001677"
     CDS             complement(8339..9313)
                     /locus_tag="OO464_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6226859.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="OO464_001678"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="OO464_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6226860.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="OO464_001679"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="OO464_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6226861.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="OO464_001680"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="OO464_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6226862.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="OO464_001681"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="OO464_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6226863.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="OO464_001682"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="OO464_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6226864.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="OO464_001683"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="OO464_001683"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6226865.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="OO464_001684"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="OO464_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6226866.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="OO464_001685"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="OO464_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6226867.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="OO464_001686"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="OO464_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR6226868.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /locus_tag="OO464_001687"
     CDS             23180..23383
                     /locus_tag="OO464_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6226869.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="OO464_001688"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="OO464_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR6226870.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="OO464_001689"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="OO464_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6226871.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="OO464_001690"
     CDS             26388..26660
                     /locus_tag="OO464_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6226872.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="OO464_001691"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="OO464_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6226873.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27344..27814)
                     /gene="argR"
                     /locus_tag="OO464_001692"
     CDS             complement(27344..27814)
                     /gene="argR"
                     /locus_tag="OO464_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6226874.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28249..29187
                     /gene="mdh"
                     /locus_tag="OO464_001693"
     CDS             28249..29187
                     /gene="mdh"
                     /locus_tag="OO464_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6226875.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29249..30316)
                     /gene="degS"
                     /locus_tag="OO464_001694"
     CDS             complement(29249..30316)
                     /gene="degS"
                     /locus_tag="OO464_001694"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6226876.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="OO464_001695"
                     /pseudo
     CDS             complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="OO464_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31907..32305)
                     /locus_tag="OO464_001696"
     CDS             complement(31907..32305)
                     /locus_tag="OO464_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6226877.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32499..33626
                     /gene="zapE"
                     /locus_tag="OO464_001697"
     CDS             32499..33626
                     /gene="zapE"
                     /locus_tag="OO464_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6226878.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33845..34273
                     /gene="rplM"
                     /locus_tag="OO464_001698"
     CDS             33845..34273
                     /gene="rplM"
                     /locus_tag="OO464_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6226879.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34289..34681
                     /gene="rpsI"
                     /locus_tag="OO464_001699"
     CDS             34289..34681
                     /gene="rpsI"
                     /locus_tag="OO464_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6226880.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35076..35714
                     /gene="sspA"
                     /locus_tag="OO464_001700"
     CDS             35076..35714
                     /gene="sspA"
                     /locus_tag="OO464_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6226881.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35720..36217
                     /gene="sspB"
                     /locus_tag="OO464_001701"
     CDS             35720..36217
                     /gene="sspB"
                     /locus_tag="OO464_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6226882.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36260..37627)
                     /gene="dcuC"
                     /locus_tag="OO464_001702"
     CDS             complement(36260..37627)
                     /gene="dcuC"
                     /locus_tag="OO464_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6226883.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9116 a         9414 c         9900 g         9198 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatgcc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaactggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatctg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagagt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgggcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgttcg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctca ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gcctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 cagacaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacctggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ctggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgctttt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag gacaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca ctaattattg
    27061 aactaattaa taccacacta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt
    27121 aataacaaaa aagtataaaa aacacggtta tttattatta attatagtat tttcaattag
    27181 ataatgaatt ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg
    27241 tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg
    27301 ataagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
    27361 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa
    27421 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
    27481 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
    27541 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
    27601 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
    27661 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg
    27721 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
    27781 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
    27841 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
    27901 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
    27961 acggcatgca aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc
    28021 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
    28081 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
    28141 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
    28201 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
    28261 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
    28321 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc
    28381 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
    28441 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt
    28501 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
    28561 gttgcgaaaa actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
    28621 gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
    28681 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
    28741 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
    28801 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
    28861 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
    28921 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc
    28981 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc
    29041 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
    29101 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc
    29161 ctgggcaaag agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag
    29221 actccggttt ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga
    29281 cctgcagcgt taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc
    29341 gaatttccgc cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg
    29401 aaataatcag atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt
    29461 cattaaccac gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga
    29521 tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat
    29581 ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc
    29641 catcgttact cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca
    29701 ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc
    29761 gcccggtcgg gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct
    29821 gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac
    29881 gtgcattaat tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat
    29941 cggttagaga gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga
    30001 cgatgatctg atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat
    30061 ccatgattac accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca
    30121 aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc
    30181 tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca
    30241 gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta
    30301 agagcttcac aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg
    30361 agttaaccgg acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag
    30421 atagatgctt tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag
    30481 cactttacgc atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc
    30541 gtctttttgc aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat
    30601 acctttgccg ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggccg
    30661 acgaagaggt gctggtatca agcgtcactt ctacttccag tggtttgccg ttacgcagca
    30721 ggccaagctt cactttcgtg cccggctctg tagtcgcgat acgagagcgc aactcagcaa
    30781 agctattcag cggtttgccg ttgaggctgg taataatatc gcccgctttg acgcccgctt
    30841 tcgctgagcc agaacttggc aacacttcgc tgacaaacgc gccacgctgc acgtcaaggt
    30901 tgaaggcttt ggcgatatcg gcactcatct cggtgccttt gatgcctaac aaaccgcgtt
    30961 tgatttcacc aaagtcgata agctgctgcg ccagtgttcg cgccatatta ctggggatgg
    31021 caaatccaat cccgacgctc ccgccgccag gcgcaaggat tgcagtgttg atgccaatta
    31081 actcaccgtt aaggtttaat agtgcaccgc cggagttacc gcggttaatg gaagcatctg
    31141 tctggataaa gttttccaga ccttcaagat tcaacccgct gcggcctaat gcggaaacaa
    31201 tgccagaggt ggcggtttgc ccaaggccaa atgggttacc gaccgctacg gcaaaatcac
    31261 cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt taatttgctc gggttttgaa
    31321 tttgtaacag ggcgatatcg ctctggtcat cgctaccaat cagttttgca tcaaactcgc
    31381 gcccatcatt gagctgaata ctgattttct gtgcctgatt aatcacatgg ttgttggtca
    31441 gcacatagcc tttactggcg ttgatgatga caccggaacc taaaccttcg aagggttgtg
    31501 caggttgatc cggtaaatca tcaccaaaaa actttttgaa ttcttccggg attttctgtc
    31561 cctgactggc cgttccttcc acccgtacgc tcaccactgc cggaagcact ttttccagca
    31621 ttggagccag actggggaga ggggcctgat cggcaacctg gcctggaatc gacgcgacgg
    31681 cctgaaatga cgccgagaga gttaacccgg cacttaacgc taatgcactc aacagctggg
    31741 tttgtttttt cattattcct gctctcgtac ctgaatgata agaaaagaga ttcaaaacag
    31801 tttgatgtta ttgaattttc agacggctaa caatgagaca ccagattaaa tgatagtcgg
    31861 ggagggagag aagaggaggg cgcaatggct gcgcccgaaa aataaattag tcgcgcttcg
    31921 cgccagtacg cagcaggccg gatgcgcctt cagaatagtc gcgaggcacc tgcaccggtg
    31981 cctgatcgtt gctggcttca gactctgcca gacgattacg gaacgggttt gcttcagcag
    32041 acagttccgg cagcaggctg ctggagcttt ttgccatgtg ctgatacagc tggcgatagt
    32101 cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa gtggctaacc agctcttcgc
    32161 gatactcgtc cagttcagct ttattctttt ccagttcgta ctgcaacgcc tgttgctggc
    32221 gtagtttacg attaccaaaa cgcatggcca cagcaccaat aatgatgccg acgactaacc
    32281 caattagcgc atattcccag gtcatgaaca tctcccgttg tcttttgttt ccgtagggtg
    32341 ttggcttcag gctccatgcc tgcggctgat tatgccacta taaccgttaa ttccacagaa
    32401 gtggaatccc gactgcatat cgcgtagtgt agaacggcct ttttttcgtc aaccgtgaac
    32461 aacggcgcac cgattattca aggaataaca ataagatcat gcaaagcgtt accccaacat
    32521 cgcaatacct gaaggcgctc aatgaaggca gccatcaacc cgacgacgtt caaaaagagg
    32581 ccgtcagccg cctggaaatt atttatcaag aactcatcaa tagcaggcca ccagccccca
    32641 ggacgagtgg gctaatggcg cgggtcggta agctgtgggg taaacgcgaa gacacaaagc
    32701 atacgccagt gcgtggctta tatatgtggg gcggtgtagg acgcgggaaa acctggctga
    32761 tggacctttt ctatcaaagc ctgccgggag agcggaaaca gcgcctgcac tttcaccgtt
    32821 ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg gcagaccgat ccgctggaaa
    32881 ttattgccga tcgctttaaa gccgaaactg acgtgctctg ttttgacgaa ttttttgttt
    32941 ctgatattac cgacgccatg ctacttggcg gtctgatgaa agccctgttc acccgcggta
    33001 ttaccctggt agcgacgtca aatattccgc cggacgaact ttatcgaaat ggcctgcaac
    33061 gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca ttgtgatgta atgaacgtgg
    33121 acgctggtgt tgattatcga ctgcgtacac tcactcaggc gcatctgtgg ctttcgccac
    33181 tcaacgatga aacccgggcg cagatggata aactatggtt ggcgctggcg ggggtgaaac
    33241 gagaaaattc accgacgtta gaaatcaacc atcggccatt ggcgacaatg ggcgtcgaga
    33301 accagacgct ggcggtttct tttactacgc tgtgcgtcga cgcccgcagt cagcatgact
    33361 atattgcgct ctcacgcctg tttcacacag taatgttgtt tgatgtgcca gtcatgacgc
    33421 ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt ggatgagttt tacgagcgcc
    33481 atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga aatttatcag ggtgagcggc
    33541 tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga gatgcaaagc gaagagtatc
    33601 tgaagcgcga gcatttggcg ggttaaaacc tgccacaaat cacaaaaagg ggtcgatctt
    33661 tgaccccgac ttctctataa tcctgcgacc ccacgttaca agaaagtttt tttcccaaaa
    33721 ctttttgtgt gctggcatag gctattcgaa ggggtaggtt tgccggactt tgtcgtgtga
    33781 acctcaacaa ttgaagacgt ttgggtgttc accaacgtgt aactatttat tgggtaagct
    33841 tttaatgaaa acttttacag ctaaaccaga aaccgtaaaa cgcgactggt atgttgttga
    33901 cgcgaccggt aaaactctgg gccgtctggc tactgaactg gctcgtcgcc tgcgcggtaa
    33961 gcacaaagcg gaatatactc cgcacgtaga taccggtgat tacatcatcg ttctgaacgc
    34021 tgacaaagtt gctgtaaccg gcaacaagcg tactgacaaa gtgtactatc accacaccgg
    34081 ccacatcggt ggtatcaaac aagcgacctt tgaagagatg attgctcgcc gtcctgagcg
    34141 tgtgattgaa atcgcggtta aaggcatgtt gccaaaaggc ccgctgggtc gtgctatgtt
    34201 ccgtaaactg aaagtttacg cgggtaacga gcacaaccac gcggcacagc aaccgcaagt
    34261 tcttgacatc taatcgggat tataggcaat ggctgaaaat caatactacg gcactggtcg
    34321 ccgcaaaagt tccgcagctc gcgttttcat caaaccgggc aacggtaaaa tcgtaatcaa
    34381 ccaacgttct ctggaacagt acttcggtcg tgaaactgcc cgcatggtag ttcgtcagcc
    34441 gctggaactg gtcgacatgg ttgagaaact ggacctgtac atcaccgtta aaggtggtgg
    34501 tatctctggt caggctggtg cgatccgtca cggtatcacc cgcgctctga tggaatacga
    34561 cgagtccctg cgttctgaac tgcgtaaagc tggcttcgtt actcgtgacg ctcgtcaggt
    34621 tgaacgtaag aaagtcggtc tgcgtaaagc acgtcgtcgt ccgcagttct ccaaacgtta
    34681 attggcttct gctacggcag aaaacaattt gcgaaaaaac ccgcttcggc gggttttttt
    34741 atggctaaat tctgaatcag cgtaaaaaat gggaagttgc tttttgctgc cgtctgaaag
    34801 acaggtaaaa caaaaaccac atcgccaata agggtctaag tcaactattt cagactaaag
    34861 cgcatctctt tttccccatt tccggcatcc actcaccaca aaggtcgcaa aatctggtaa
    34921 actatcatcc aattttctgc ccaaatgtcg ggtattgctc attttttgtt tgattttcga
    34981 acaaagagag tcgttcctta ttgggtaaca caacttctga ctggccacct ggtggctggt
    35041 agcagtaaaa attctgacta tacctggagg ttttcatggc tgtcgctgcc aacaaacgtt
    35101 cggtaatgac gctgttttcc ggtcctactg acatctatag ccatcaggtc cgcattgtgc
    35161 tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga aaaggacaat ccgcctcagg
    35221 atctgattga cctcaacccg aatcagagcg ttccgaccct ggtggatcgt gagctgaccc
    35281 tgtgggaatc tcgcatcatt atggaatatc tggatgagcg tttcccgcat ccgccactga
    35341 tgcctgttta cccggtagct cgcggtgaaa gccgtctgta catgcatcgc atcgaaaaag
    35401 actggtacac gctgatgaac actatcatca acggttcagc ttctgaagca gatgccgcac
    35461 gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt cttcggtcag aagccgtact
    35521 tcctgagcga tgagttcagc ctggtcgatt gctaccttgc tccgctgctg tggcgtctgc
    35581 cgcaactggg catcgagttc agcggcccgg gtgcgaaaga gctgaaaggc tatatgaccc
    35641 gcgtctttga gcgtgactct ttccttgctt ctttaactga agcagaacgt gaaatgcgtc
    35701 tgggccggag ttaatctgta tggatttgtc acagctaaca ccacgtcgtc cctatctgct
    35761 gcgtgcattc tatgagtggt tgctggataa ccagctcacg ccgcacctgg tggtggatgt
    35821 gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt gacgggcaaa tcgtactcaa
    35881 cattgcgccg cgtgctgtcg gcaatctgga actggcgaat gatgaggtgc gctttaacgc
    35941 gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg gctgccgtgc tggctatcta
    36001 cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa gctgcctacg atgaagatac
    36061 cagcatcatg aatgatgaag aggcatcggc agacaacgaa accgttatgt cggttattga
    36121 tggcgacaag ccagatcacg atgatgacac tcatcctgac gatgaacctc cgcagccacc
    36181 acgcggtggt cgaccggcat tacgcgttgt gaagtaatac aaaacaggcc caggcggcct
    36241 gttttgtctt tttaatgact taataaaata gcgtgattgt ggcaatcata ttcaccacga
    36301 aaccgactgc catggggatc gctgtccgct tcaccaccgc aaaaggcgaa acgcctgcaa
    36361 ttcctgacgt aacgaccacc accgcagtaa tcggcgaaac cgcgcgcgct agcgtcgtgg
    36421 caaaatgcat cggcataatc attacaaccg ctggtacatg tagtccggct gcgatattcg
    36481 gaataagact ggcaaatgac ataaacggcg cattgccaga gcccatcaca atggcacaaa
    36541 tggcaatgac tagcgccata ataatcatca cgccaatacc gcccagacca gaatgctccg
    36601 cacccctgat aaccgcatca acagtgccaa tcgtcgttaa gcctttcgca aatatttcac
    36661 ccgcgaccac cagcgttacc acgttgacaa actgcgtacc catgccgtca aaaaacgcct
    36721 gcacatcgtc cattgtttcg cgcaagttat gcttgcggaa gaactcaaca aacatcgtca
    36781 ccgtcaaact tagtaacatc accaccacca gatgcagttc tgactgcatc agcccgacgt
    36841 gggcgaggaa cagcgagccg agcatcagga ttaacggcat cacaggtaaa atggcgtaat
    36901 agagcggcgg gacattatcg agagtttttt gctctgcctg ttcgtgattg atatttttat
    36961 cttttttgtc aaaagcgcgt tgcacgaaaa agtgggagat cgccaccgag ataatgacgc
    37021 aagaggcgac cggaagctgg tagtggaaga agtatgtggc aattttcatt cccgcgacct
    37081 gggctgcaaa aatggagttc gtttccagaa tcccccactc aatggacatc gtggttgcga
    37141 taaccgccac cgcagagagg cgacttactc ccagactcac cagcgtcgga aataaggtga
    37201 ccatcagcaa catacccaga ccggaggcgc tggtaataaa ctgcgccatg atttggccga
    37261 tgacgtaagt tgccgacaga ataatatacg gcgagcgaat gagttttaac gggcggctta
    37321 gcaagctcac catcgcgcaa ctggctccta tgcgctccat gtagcgggca taaccgccca
    37381 ccgccataat cgacagcccc aatccggcaa tgcggttgga cagcatgcgc aggatttcat
    37441 tataaatgtc gacgatcaag tagccgctgc ttttggcggg atcgagtaca ccaccgaacc
    37501 ctaaccagac tccgcacatc atcaggaaga cacctgcggc agccagcacc acctgaggtt
    37561 tgtagttttt caggatcaaa tagcccatcg taattaatac gatgacagat ataattatgc
    37621 cgaacatt
//
LOCUS       DAJTUI010000031        36588 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTUI010000031 DAJTUI010000000
VERSION     DAJTUI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.031"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(157..1392)
                     /locus_tag="OO464_001703"
     CDS             complement(157..1392)
                     /locus_tag="OO464_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6226884.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1539..1615)
                     /locus_tag="OO464_001704"
     tRNA            complement(1539..1615)
                     /locus_tag="OO464_001704"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Pro,seq:tgg)
     gene            complement(1658..1744)
                     /locus_tag="OO464_001705"
     tRNA            complement(1658..1744)
                     /locus_tag="OO464_001705"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1708..1710),aa:Leu,seq:cag)
     gene            complement(1765..1840)
                     /locus_tag="OO464_001706"
     tRNA            complement(1765..1840)
                     /locus_tag="OO464_001706"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1805..1807),aa:His,seq:gtg)
     gene            complement(1899..1975)
                     /locus_tag="OO464_001707"
     tRNA            complement(1899..1975)
                     /locus_tag="OO464_001707"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1939..1941),aa:Arg,seq:ccg)
     gene            complement(2078..3463)
                     /locus_tag="OO464_001708"
     CDS             complement(2078..3463)
                     /locus_tag="OO464_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6226885.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3654..4394)
                     /gene="rffM"
                     /locus_tag="OO464_001709"
     CDS             complement(3654..4394)
                     /gene="rffM"
                     /locus_tag="OO464_001709"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6226886.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4397..5749)
                     /gene="wzyE"
                     /locus_tag="OO464_001710"
     CDS             complement(4397..5749)
                     /gene="wzyE"
                     /locus_tag="OO464_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6226887.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5746..6825)
                     /gene="rffT"
                     /locus_tag="OO464_001711"
                     /gene_synonym="wecF"
     CDS             complement(5746..6825)
                     /gene="rffT"
                     /locus_tag="OO464_001711"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6226888.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6822..8072)
                     /gene="wzxE"
                     /locus_tag="OO464_001712"
     CDS             complement(6822..8072)
                     /gene="wzxE"
                     /locus_tag="OO464_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6226889.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8074..9204)
                     /gene="rffA"
                     /locus_tag="OO464_001713"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8074..9204)
                     /gene="rffA"
                     /locus_tag="OO464_001713"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6226890.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9209..9883)
                     /gene="rffC"
                     /locus_tag="OO464_001714"
                     /gene_synonym="wecD"
     CDS             complement(9209..9883)
                     /gene="rffC"
                     /locus_tag="OO464_001714"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6226891.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9861..10742)
                     /gene="rfbA"
                     /locus_tag="OO464_001715"
     CDS             complement(9861..10742)
                     /gene="rfbA"
                     /locus_tag="OO464_001715"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6226892.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10761..11828)
                     /gene="rffG"
                     /locus_tag="OO464_001716"
     CDS             complement(10761..11828)
                     /gene="rffG"
                     /locus_tag="OO464_001716"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6226893.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11828..13090)
                     /gene="wecC"
                     /locus_tag="OO464_001717"
     CDS             complement(11828..13090)
                     /gene="wecC"
                     /locus_tag="OO464_001717"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6226894.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13087..14217)
                     /gene="wecB"
                     /locus_tag="OO464_001718"
     CDS             complement(13087..14217)
                     /gene="wecB"
                     /locus_tag="OO464_001718"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6226895.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14273..15319)
                     /gene="wzzE"
                     /locus_tag="OO464_001719"
     CDS             complement(14273..15319)
                     /gene="wzzE"
                     /locus_tag="OO464_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6226896.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15331..16434)
                     /gene="wecA"
                     /locus_tag="OO464_001720"
     CDS             complement(15331..16434)
                     /gene="wecA"
                     /locus_tag="OO464_001720"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6226897.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16674..17933)
                     /gene="rho"
                     /locus_tag="OO464_001721"
     CDS             complement(16674..17933)
                     /gene="rho"
                     /locus_tag="OO464_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6226898.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18018..18119)
                     /gene="rhoL"
                     /locus_tag="OO464_001722"
     CDS             complement(18018..18119)
                     /gene="rhoL"
                     /locus_tag="OO464_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6226899.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18260..18589)
                     /gene="trxA"
                     /locus_tag="OO464_001723"
     CDS             complement(18260..18589)
                     /gene="trxA"
                     /locus_tag="OO464_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6226900.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18720..19985
                     /gene="rhlB"
                     /locus_tag="OO464_001724"
     CDS             18720..19985
                     /gene="rhlB"
                     /locus_tag="OO464_001724"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6226901.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20119..21603
                     /gene="gppA"
                     /locus_tag="OO464_001725"
     CDS             20119..21603
                     /gene="gppA"
                     /locus_tag="OO464_001725"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6226902.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21650..23671)
                     /gene="rep"
                     /locus_tag="OO464_001726"
     CDS             complement(21650..23671)
                     /gene="rep"
                     /locus_tag="OO464_001726"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6226903.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23758..24039
                     /gene="ppiC"
                     /locus_tag="OO464_001727"
     CDS             23758..24039
                     /gene="ppiC"
                     /locus_tag="OO464_001727"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6226904.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24085..25560)
                     /gene="ilvC"
                     /locus_tag="OO464_001728"
     CDS             complement(24085..25560)
                     /gene="ilvC"
                     /locus_tag="OO464_001728"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6226905.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25710..26600
                     /gene="ilvY"
                     /locus_tag="OO464_001729"
     CDS             25710..26600
                     /gene="ilvY"
                     /locus_tag="OO464_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6226906.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26597..28141)
                     /gene="ilvA"
                     /locus_tag="OO464_001730"
     CDS             complement(26597..28141)
                     /gene="ilvA"
                     /locus_tag="OO464_001730"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6226907.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28144..29994)
                     /gene="ilvD"
                     /locus_tag="OO464_001731"
     CDS             complement(28144..29994)
                     /gene="ilvD"
                     /locus_tag="OO464_001731"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6226908.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30059..30988)
                     /gene="ilvE"
                     /locus_tag="OO464_001732"
     CDS             complement(30059..30988)
                     /gene="ilvE"
                     /locus_tag="OO464_001732"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6226909.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31008..31271)
                     /gene="ilvM"
                     /locus_tag="OO464_001733"
     CDS             complement(31008..31271)
                     /gene="ilvM"
                     /locus_tag="OO464_001733"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6226910.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31268..32914)
                     /locus_tag="OO464_001734"
     CDS             complement(31268..32914)
                     /locus_tag="OO464_001734"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6226911.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32917..32967)
                     /locus_tag="OO464_001735"
     CDS             complement(32917..32967)
                     /locus_tag="OO464_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226912.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33053..33151)
                     /gene="ilvL"
                     /locus_tag="OO464_001736"
     CDS             complement(33053..33151)
                     /gene="ilvL"
                     /locus_tag="OO464_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6226913.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33503..34982
                     /locus_tag="OO464_001737"
                     /pseudo
     CDS             33503..34982
                     /locus_tag="OO464_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(35007..35345)
                     /gene="maoP"
                     /locus_tag="OO464_001738"
     CDS             complement(35007..35345)
                     /gene="maoP"
                     /locus_tag="OO464_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6226914.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35464..36303
                     /gene="hdfR"
                     /locus_tag="OO464_001739"
     CDS             35464..36303
                     /gene="hdfR"
                     /locus_tag="OO464_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6226915.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36399..36474)
                     /locus_tag="OO464_001740"
     tRNA            complement(36399..36474)
                     /locus_tag="OO464_001740"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36439..36441),aa:Trp,seq:cca)
     gene            complement(36483..36559)
                     /locus_tag="OO464_001741"
     tRNA            complement(36483..36559)
                     /locus_tag="OO464_001741"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36523..36525),aa:Asp,seq:gtc)
BASE COUNT         8940 a        10012 c         9217 g         8419 t
ORIGIN      
        1 tgccaccgca cgtaaaggct ttgccggata cgatcaccca aaccagtatc cacagaacgc
       61 tgcaatttat tgaatttgca cgatcatgta ggccggataa ggcgtttacg ccgcatccgg
      121 caatcaaccg caggcggctt ccaaatggcc gcttttttac ttattcacca ccatcaaatg
      181 tgcctgcata atgtcactgg ccgggcgacc gtgcgccagc aaatcagcca ttgctttaag
      241 atatggcggt agatggcgga aataacgctg atacccggca cacaaataat tcagccccgg
      301 tttgccgctg gcatcgagca tgaagcggtg tttcgggcag cctccccaac atgcttttaa
      361 cacgttacaa ctgcgacact gcgccggtaa ttgcttaaat ttatcttcac caaacacctg
      421 ctgttgcggg gaatcgatca tttctgcaat tgtttgctga tgcatattcc ccagccgata
      481 ttgcggataa acatagtgat cgcaggcgta aacgtcgccg ttgtgctcaa caatcaccga
      541 gcgcccacag gttggctgat gatggcaaac cgcacccggc gcaccgacaa aattggcaaa
      601 cgcccattcg atattcatca cgaaaatctt gccgacgtcg cgtttgatcc agtggtcaaa
      661 aatcgccacc agaaattcac caaattcatc ggggcgcaca gaccattccg tcagttcccc
      721 ctgaatatca ccaggcgcat gtagtttcag tccatcgcgg acagctgttt catcagccag
      781 gcgctcgacc accggaataa actggatgaa ttcgactccc gcatcgcaca aaaaatcata
      841 aacctgtaac ggttgctgcg cgctggtgcg gttgacgcag accagcacgt tatagtcgac
      901 atgatgtttt tgcaggagcg tcagggcacg catcaccagt ttatgcgtgg gtctgccgcc
      961 tttggtcacg cgatattgat tgtggatctc agccggacca tccagcgata acccaacaag
     1021 aaaattattt tctgccagaa atgcacacca ttcgtcatcg agcagcacgc cgttagtctg
     1081 gaaactgtta cttatcttcc tgccagcacc atatttcgcc tgtagcgcca cggcacggcg
     1141 gtaaaactcc agcccgagta gcgttggttc gccgccctgc caggtaaaag ccacttcgtt
     1201 ttgcagttcg ctggcggcga tatagtgacg gatatacgct tccagcgtgt catcgtccat
     1261 atgcgtgact ggcttttcgc ggtaaaggga ttgtttttcg agataaaaac agtagtcaca
     1321 gttcagatta caatcggaac cgctcggttt cgctatcaca tgaaaagcac gcgttggaac
     1381 ctgttgcagc atggtcgctc cttgcctgaa gtcatccttc aatacgttaa gggcgaaatc
     1441 ataccgtggg gcaatgagtt attacgggcg tttattgcga agggagggtg acgaaatgca
     1501 cagaaaacaa aaaaccccgc cgaagcgggg ttcaaaattg gtcggcgaga gaggattcga
     1561 acctccgacc cactggtccc aaaccagttg cgctaccaag ctgcgctact cgccgaaata
     1621 ctgctttttg aatttttagt tcaattcttt aaagtcgtgg tgcgaggggg gggacttgaa
     1681 cccccacgtc cgtaaggaca ctaacacctg aagctagcgc gtctaccaat tccgccacct
     1741 tcgcattgtc acaacttcta ataatggggt ggctaatggg attcgaaccc acgacaactg
     1801 gaatcacaat ccagggctct accaactgag ctatagccac cactacaaat cttgttacgc
     1861 ggtattacta ccaccgcagc tcaagcgctg ggactaaatg gcgcgcccga caggattcga
     1921 acctgagacc tctgcctccg gagggcagcg ctctatccag ctgagctacg ggcgcttagc
     1981 gccgttgcgg gggtggataa tacggacttc ccacccctct gtctagtccc tttttaaata
     2041 aattgctcgt ttggttacgg tttgtgcgtt ttgctgctta ttcctccagt ttatgcgctt
     2101 taccgtggcg attaagccca aaaactttat aaatcgccgt caccgccagc ataaagatga
     2161 tgccgacaaa cagcgacata cgcgtatctt cattaaagta catgccgatc aaaacgcaaa
     2221 tcaggaatgc cattgttacg tagttggccc acgggaacag gattgagcgg aacggatggc
     2281 tggcaatcgc cgctttatgt gcacgccgaa aacgcagctg gcttatcaat atcacaaacc
     2341 atggcaccat ccccggaagc acgctggcac tgtagacgta gacaaacaca cgctgcggat
     2401 tgggaatgat gtagttcagg catgagccaa ttagcagaat agcaatagat actgccacac
     2461 ccgcaaccgg tacgccgtga cgggaaactt ttgccattgc cgccggtaac tgacggtttt
     2521 tcgccagtgc gtagagcata cgtccgcaac tgtacatgcc gctgttacag ccagagagcg
     2581 cagccgtcag caccacaaag ttgataatgc ccgccgctgc ggtaataccg attttggcaa
     2641 aagtcagtac gaacgggctg ccgttgctgc caatttcatt ccacgggaag atggtgacga
     2701 taacgaaaat cgcgcctacg tagaaaatca ggatccgcca cagcactttg cctacggcac
     2761 tgcgcagcgt cacctgcgga ttcttcgctt caccggcagt aatgccaatc agctccacgc
     2821 cctggtagga cgccaccaca atacacagag cggtcaggaa ccctttccag ccacccgcaa
     2881 agaaaccgcc atgctctgtg agattgctaa aaccaatcga ctgcccgcca ttgccaaagc
     2941 caaagaaaat cacgcccagg ccaatgacaa tcatcacgat aatcgtggtg actttgatca
     3001 tcgcgaacca gaactcgatt tcgccgtaca accgtaccgc cgccagattc gccaacgcca
     3061 ccagcgccac cgcgatcaat gcgggtatcc actgcgccat ctccggggac caaaactgga
     3121 cataaacgcc aatggcggtg atttctgaga tccccaccgc catccacata aaccagtaag
     3181 accaggcggt gagatagcca aaaaacgggc tcatataacg atgcgcataa acggcgaacg
     3241 aaccggtaac cggttcgagg aacaacattt cgcccattga acgcatgatg aaaaaaacga
     3301 acagcccggc gatgatatag gccaacaata cggatggccc ggcccatttc agggtactgg
     3361 cggcccccat aaacaggccg acgccaatgg tgccccccag ggcgatgagt tcgatatgtc
     3421 gagcttccag cccacgctgt agctctggtt tgttatctgc cataaatcct cgtgttgtgt
     3481 ttgcatgctt tccggtgtta ccggttatcg ttatgggtac atcgagtgtt gcgaatgttt
     3541 tcgtaattca ggagaaatgg caaataaagc attaaaaatt tgaatgcttt gtgtaataaa
     3601 aaagcagaca ggcgacgaag tgaccactcc gtcgctttac aaagagagga aaatcagagg
     3661 ttgccggtgt agtgccagcg taaataacgc agcaaacgaa gctgacgctt aatgcggctc
     3721 ggctgcgaaa gcaggcggta gagccactcc agtcccagcg tttgccagat tttcggtgcg
     3781 cgttttacgt gaccggtgaa aacatcgtaa gtcccgccaa cgcccatata cagcgcatct
     3841 ggatgcacca gacggcagtc gcgcatgaag atctcctgct ttggcgatcc catcgcaacg
     3901 gtgacgattt gcgctccgct ggcatgaatg cgttcaaaca gcgcctgacg ctgctcgggt
     3961 ttaaaataac catcctgact gccaacgata ttcacattcc attggttgcg cagtttagct
     4021 tcagtttgcg ccagcacttc aggtttaccg cccacaagaa ataccggcgt cccttctttg
     4081 cctgcgcgcg ccatcagctc ttcccagaga tcggcaccgg caacgcggga aacctgcgcc
     4141 tgcgggtact ttttacgtac tgaacgtaca atgctgatgc catccgcata tttaaattcg
     4201 gcagcgttaa ttaactccct gacctcggcg ttatcttcaa tggtcagcat tttttcggca
     4261 ttaatggcaa ccagcgttcc ctgcttaagc tgcccgtcag caaacagata atcgagggca
     4321 tgctgcatat cacgccaacc aatcaactgt aagccacgca gcgtataggt cggtgccgtg
     4381 gtgttgttat tcattgttat ccttcaacct gcgtccggag cgatgatttt gtacgtttat
     4441 gaatgagtcc ggcgctttca aaaagccagt acaacagttt tgcgatcatc agacatgcgc
     4501 cgaagaccac gataaaaaag actacgcgtg agacaaacga atccagccct tcacgtgcca
     4561 gcacgatcat attgaagatc gccccaaagc agaaactgtg caatatcgca gccttatagc
     4621 gattagtctc gcggttgccc agctcataca gccagtcgaa ccatttgatg atcagtccaa
     4681 ccacaatcgc cccgagtggg atgaacaacg cgccgcccat caccaccagt gagcctataa
     4741 gcgtaggcga gatcgccagt ccggagtggt tattcagtac ttcccaggta aagtaattgg
     4801 ctgagttcag caccatactc gggcgccccg gccacagcca ggaagggata aagacataga
     4861 aatcgcggac aattggagcc aggccctgga agtcgatgtt gtcgtagttc tgcaacagca
     4921 acgccagatt ctcccacggt gaaaaggtgt cgcgagtgag atagagaaac gtatagaacg
     4981 cttcatcgcc gctcacattc attccatagc gttttaacgc cagccagaac atgccaacaa
     5041 tccccagcac gcccgccgcc gccagcatcc acaacgaaat ccagccgcga ataatgccaa
     5101 taaacaggaa gatagcgaat gcgatgatga tattggcgcg agtgccgccg acaatcatat
     5161 aagtcagcaa gccaaaggcg accgtgctga cgaggaaaaa cagccacgct ttgctgtcct
     5221 ggcgcagaaa gtagaccacc agcatcgccg ggatgaaaaa gtaaaagaag cgttttaacg
     5281 ccacgccgga gacttcactg gaaaagatct gactgtagga gttaagccgg aacagcaaaa
     5341 agccgttgtg catgaagaag atgccgacgc ttaccagcgc gatacccatc aggatcaccc
     5401 acgtaagatt ggtctccacg cggttcatgg taaacagcgg acggcgcggt acatcagcaa
     5461 cgcgtttgcg taggcgggtt ttgtaggtga catagtaaac cgcgtagaag cagcccgcag
     5521 aaagcaacac ctgcaacaag atttctggcg gcgcgacacc aacatcaaag cgaaatacca
     5581 gcacgctggt cagcgggaag ccgaagaaaa aggtgagcaa aaacagcaat gaaaagaaga
     5641 cattgaagtt aaagcgcaca cggcgaaact caaaccaggt cagcgtggca ataaacagcg
     5701 tgcagagcag ccagaccaca aacaggccac tgaattgcag cagactcatg cgacctcccc
     5761 ggcggcaatc gccaacgccc gctgccagcc ttgtagatag ttagggctaa agaaggcaat
     5821 ggtgttttta tccgccgacg ccaactggcg ctgcgcttca cgcacaatat cctcgttgag
     5881 atcgtcggta gtaaacagca ctggcaaatg ctgttccgtc atatcctgcc agaacggatt
     5941 ttccctgtta agcacacaag gaatgcccgc ctgaatcagt aagcaaagcg taccaatgcc
     6001 ctgctggcgg gcaaaaataa agtaaccaag atcgcactga cgaagtagcg ccagataggc
     6061 gtcaaattcc agtttttcgc tcaaaatttg cagattttct tcgctgaata actccagccc
     6121 cgcctgacga acttcctcaa tgtacgcttc gttattaggc ggatatccca tcggcaccac
     6181 cacttttacc gtatcgccaa attgctgatg aacggcacgt aaagcggcga catgctcatt
     6241 gctgcggtcg ccggagttcc ccaccagaat ggtcattttt ccttcacgtt gccgatcgtt
     6301 cgccatcgta ttgagagaag ggtccatccg cgtcgggaag tacagcagtt ctccccgcac
     6361 ctttgggtgt gttttggcaa aaaagctcaa atcgccgcgg gtggcaaata cacagccgac
     6421 tcgcttttgc gccaggcgac gtagtgggta aaaaagctta tatctcaagc cactggaaag
     6481 ctcatacagg tctgcccccc agatatgcca gaaaaactgg ctgggcttaa tgccaccact
     6541 cagcagagcc agccacagtg tgggattgaa ctgaccgtgg aaaaaaaatc gctgctgacg
     6601 gttagctttt gctttcgcga tgaccgcttc cgccagcgat tttttcccag ggaaaaattg
     6661 cacagaaagc gccggacagc tatcacttaa gccgtcgtcc ttgccaacaa ccataaactc
     6721 gcgcgcgtgc tcgctcgtcg cggccagcgc gtcattgaaa aaccgcaaaa cggttcggtt
     6781 atggtgaggg atatccgatc ccagtacgtg aatcagtaca gtcatgcccg cctacgccag
     6841 agtaaaaaca cgccacaaca aagagaaaaa tagacgatat aagttgccat atatgcctgc
     6901 gccgcgccca gtgcaccatg cgcagggatt agccagtggg caaataccat caataaagtg
     6961 aactggctga cttccgccag aatataaaac cgcagtgacg ctttggcgat caccagataa
     7021 ccaaagacat aagcgcccac ttttaacaca tcacccacta actgccaggc aaagagatcg
     7081 cgcatagcgg taaatttatt cgacaacagc aaccagatag caaaatcacg cagcaaccag
     7141 acggtaaaac tcgctgccgc cactgccggt aagacgaatt tcagcgattt aaccacttcc
     7201 cgggtgatat cgcgcttttc cgttagccgc gacaacgtgg gcagcaaata tacgctgaac
     7261 gatgccgtaa taaattgcag gtaggcatcg gaaatactgc tcaccccttg ccagatcccc
     7321 acttcatccc agctatactg cgccgccagc agtttacgca tcatgatgta agcaacaggc
     7381 aaggtcaccg acgtaatcaa cgccatgagc gtaaatttgc tcaactgccc tgccagaccg
     7441 ttatcccagc tgggtttcag atagcttaac gggatgacac cacgtttaat caacatgatg
     7501 gcggcaggaa ttaccaccag cgcgggaatc agcgccagac ccagcaacgc cccttcataa
     7561 ccgcccaaac ggtaactgac gtagtacgcg agaacgccaa tcaagctgcc gacaatcagc
     7621 gataacgcat tacctgcggc atcgcgaaag cctttcatca gcgccagtaa caggttgccc
     7681 caggcgatcc ccatttgcac cagcgccact aaacgcacca gcccctgata gtcggtatta
     7741 ccaaacaatc cctggctgat tggcgcagct gccagcacaa aaaccagcgc catcagcgta
     7801 gagaaaccaa gtaccatcgc tgatgaagtg ccgaccacgc ggcgcagctg ttgcggatta
     7861 tcatggtact gggcaacgta tttggttaca ccgttaaaga tgccagcccc ggcaagcacg
     7921 ccgagcacgg taatcaactg gcggaaattt gccgccagcc caagccccgc cggaccaaat
     7981 gacaccgcca gcaacttacc gaccagtaac ccggcaccaa tcttgaccag tgtactggcc
     8041 gccgtccaca aggacgcttt tgccaacgac atatcaggag aagtagttca acaaagtcgc
     8101 aattaccgta cgctgattga cgggcgacag gttgtagaac agcggcaggc gcagcaggcg
     8161 ctcgctctct ttggtggtgt agcgatcttc accgtggaac tcaccaaagt gttcccccgc
     8221 agggcaaccg tgcagcggaa tgtaatgaaa caccgccatg atttccgctt ctttcagaaa
     8281 gttaatcaac gcgctccggt catcaatatc ccgcagttta atgtagaaca tatgcgcgtt
     8341 ctgcacgcag ccatcgggaa tcgacggcag ctcgatacgc ccggctttcg ccagaggcgc
     8401 taacgtatcg tagtagtttt gccacagcgc cagacgttgc tggttgatac gatccgctgc
     8461 ttccagttgc gcccacaggt acgcagcttg cagatcggac atcaaatagc tggagccaat
     8521 atcgcgccag gtatatttat cgacctgacc acggaagaac tggctgcggt tagtgccctt
     8581 ttcacggatg atctcggctc gttcgattaa cgctttatcg ttaatcagcg tcgcgccgcc
     8641 ttcaccaccc gccgtgtagt ttttggtttc atggaagcta aagcagccaa tatgaccaat
     8701 ggttcccagt gcacgccctt tgtaagtgga catcacgccc tgagcggcat cttccaccac
     8761 aaacaaatta tgctttttcg ccaacgccat aatggtgtcc atttcgcagg ccacacccgc
     8821 gtaatggacc ggcacgataa cgcgcgtttt gtcggtgatc gctgcttcaa tcaacgtttc
     8881 gtcgatgttc atggtgtccg ggcgaacatc cacaaagacg attttcgcgc cacgcagcac
     8941 aaaggcattg gcggtggaga caaaggtgta gctcggcatg atcacttcat cgccaggctg
     9001 gatatcgagc agcagcgccg ccatctccag cgaagcggtg caggacggcg tcagtaacac
     9061 tttggcgctg ccaaaacgtt gctccagcca ctgctggcag cgacgggtaa aaccgccatc
     9121 gccacacagt ttgccgctac ccattgccga ctgcatatag tcgagttcgg ttcccaccac
     9181 cggcggtgcg ttaaatggaa tcatgtgatc acctgtataa ccagtacgcg gtgctttcta
     9241 cattcgcacc actttgtatg tatcgtttaa gcgcggcggt gttgcccatt tgggtcgcca
     9301 cccgcaaagt tgttttaccg cgagcatacg cccagtttag cgccgtttgc atcagctcag
     9361 cacctgcacc gcgtccagcc agcaggccaa ttcgcgcatc tgtcgcattg agttcccgta
     9421 aagagacata gccgcgaata tcgccggacg ccgcacgtag aatcagacat tgatgatcaa
     9481 aggtgccgcg cacggcattt tcaatccact gtgcataaaa gcgaccgctg gcgtcaggcg
     9541 cataccacgg cgcacgaaaa cggctttgcg caaatgcggc gctagctaac tgacgtaatg
     9601 cgggaatatc ggtctcttgt gccacgacag caccgctatc actgacattg ttcacgggta
     9661 gcgccaaatc aacttcacct tctaccaggg agaatcccag ctgttgcagg gcatccagtt
     9721 cccccgtatt tgatgccgca attttggcct gcacccgtga ccatggcgct aaggcgtctg
     9781 acgtcaggag cggtgcttca gacgtaatgc gcacgatggc gctgttaaca ccaaagaagg
     9841 cgttttccca ggttagtggc tcaatactgg cgcggacggg cacgaagtaa ctccagcaga
     9901 tattggccgt agccagtttt cgctaatgaa ctggcagcac gcttcacacc ctcgtcatcg
     9961 agccagccgt tacgccaggc gatctcttcc aggcaggcaa tcttaaagcc ctggcgtttt
    10021 tctactgtct gcacaaaagt gctggcctca atcaggctgt cgtgagtgcc ggtatccagc
    10081 caggcaaatc cgcgcccgag cagttcaacg gtcaggttgc ccgcctcgag gtacatctgg
    10141 ttgatggagg taatctccag ttcaccgcgc tccgacggct tcacctgctt tgcgtactcc
    10201 acgactttac tgtcgtagaa ataaagcccg gtcaccgccc agtttgactt cggctgtttt
    10261 ggcttttctt caagcgagat agcgcggaaa ttgtcgtcaa attccaccac gccaaagcgt
    10321 tccgggtcca tcacctgata gccaaaaacc gtcgcccctt ccgtgcgcgc cgcaacatga
    10381 cgcagcttcg gactgaagcc ctgaccgaag aagatgttat cgcccagcac cagacaagaa
    10441 ggttcaccat taaggaaggt ttcaccgatg ataaaggcct gcgccagacc gtccgggctg
    10501 ggctgttcgg catattccag ctggataccg aactcactac catcgcccag caggcgctgg
    10561 aaataacctt tatcttccgg cgtagtgatg atgagaattt cgcggatacc ggccagcatc
    10621 agcaccgaca gcggatagta aatcattggc ttatcgtaaa tcggcaacag ttgcttcgat
    10681 acgccgcgcg taatcggatg caatcgggta ccggaaccgc ccgccaggat aatacccttc
    10741 atgtttgcct ccgctgaaaa ttagcctttc agacctaaac gctcaccctg atagctgccg
    10801 tcctgcacct gcttccacca gctttcatta gccagatacc actgaaccgt tttacgcatt
    10861 ccactttcaa aggtttcctg cggcagccag ccaagttcac gggcaatttt cgaagcatca
    10921 atggcataac gcagatcatg ccccggacgg tcagcgacaa aggtgatcag gtcacgatag
    10981 tacgccacgc cgtgcggctt gttcgaagcc agttcttcca gcagctcgca aatggtttcc
    11041 acaacatcga gattcttacg ctcgttgtga ccaccaatat tataggtttc accgactttc
    11101 ccggtggtcg ccacgcaata cagcgcgcgg gcgtgatctt ccacatacag ccagtcacgg
    11161 atttgctgcc cgttgccata taccggcagc ggtttacccg ccagcgcgtt gaggatcatc
    11221 agcgggatca gtttttccgg aaagtggtaa ggaccgtagt tattcgagca gttggtgatc
    11281 agcgtcggca gaccgtaggt ccgcagccag gcgcgcacca gatggtcgct gctggctttt
    11341 gacgcggaat aagggctgct cggcgcatac ggcgtggttt cggtgaagaa atcatccgtc
    11401 gagtgcaggt caccatatac ttcgtcggtg gagatatgat gaaaacggaa cgctgatttt
    11461 ttatcttccg tcagcgtatt ccagtaagcc cgcgccgctt caagcaatgt ataagtcccg
    11521 acaatgttgg tttcaataaa cgctgccggg ccgtcaatag aacggtcaac atggctttcg
    11581 gctgccagat gcatgacaca gtctggctga tgctcagtga atacgcgtgc cagttctgcc
    11641 cgatcgcaga tatcaacttt ctcaaaggca aagcgctcgc tttgcgcgac cggtgccagc
    11701 gacatcaggt ttccggcgta ggtcagctta tcgaccacta ccaccgcatc gctcgtttcg
    11761 ttgatgatat aacgcaccag cgccgagcca ataaacccgg caccacctgt tatcagaatt
    11821 tttctcatca gcgccagact cctttggcat cgacgacata ctgctgatgg acattgtcgc
    11881 cattgataac tttgaactga ctatgatcga ccagcatcac cagcacgtct gccgttgcca
    11941 gcgcctcgtc aagctgcgcc agagtacaaa gcccggtcag ttttttcggc aactggtgga
    12001 tgttaggctc aacaaccagc gtttcgccgc tgtgccactg ggcgatcagt tcagcgattt
    12061 ccatcgccgg gctttcgcgc aggtcatcaa tattcggttt aaacgccaga ccaaagcagg
    12121 cgattttcag ttcactggct cgtttatcgg tagcagcaag gcagtcggca accgccgctt
    12181 tcacctgatc gataacccag aacggtttgt gatcgttaac ttcgcgcgcg gtacggataa
    12241 gccgcgcctg ctgggggttc tgtgccacga taaaccacgg atcaacagca atgcagtgac
    12301 cgcccacgcc agggccaggc tgaagaatat taacgcgagg gtgacgattc gccaggcgaa
    12361 tcagttccca gacgttaatc ccctgatcgg cacaaatcag cgacaattca ttagcaaaag
    12421 cgatattcac atcgcggaag ctgttttcgg tgagcttaca catttccgcc gtccgcgagt
    12481 tagtgacgac acactcacct tcgaggaaaa ttttgtacaa ttcgctggcg cgggccgaac
    12541 aaaccggcgt cataccacca atcacgcgat cgtttttaat cagctcgacc ataacctggc
    12601 ccggtaatac acgttccggg cagtaagcaa tgttgacgtc cgcctgctcg cccacctgct
    12661 gtgggaaagt cagatccggg cgcatctctg ctaaccattc tgccatcttc tcggttgacc
    12721 ccaccggcga ggtggattca aggatcacca gcgcgccttt tttcagcact ggcgcaatgg
    12781 agcgagcagc cgattcaacg taggtcatat ctggctcatg atcgccttta aacggcgtag
    12841 gtacagcaat cagccaggca tccgcttcaa ctggcgtcgt gctcgctcgt aaaaaaccgc
    12901 cttctacggc agtttttact acgctcgcca aatcaggctc gacgatatgg atttcgccac
    12961 gattgatggt atcaaccgcg tgttggttga tatcgacacc aatcacctgt ttttgccgtg
    13021 aggcaaacgc tgctgctgta ggcagcccga tgtaacccag tccgataaca gaaatggtcg
    13081 caaaactcat agtgatatcc gattattttt taacgcttcc agaatgcgag agcatgcctg
    13141 accatcacca tacgggttat gggcgcggct catagcttga tattcgtttt cgtcttttaa
    13201 aagacgcgtc acttcctcga caattcgctg cttatccgtg cctaccagac gcaccgtacc
    13261 cgccgtcacc gcttccggac gctcagtggt atcgcgcatc accagcaccg gtttccccag
    13321 cgaaggcgct tcttcctgaa tgccgcctga gtcagtcaaa atcagccagg cgtggttcat
    13381 cagccagaca aacggtaaat actcctgggg atcgatcaga atgacatttt tcacatgccc
    13441 cagaatgcga ttgaccggct ctctgacgtt cgggttgaga tgcaccggat agacaatctg
    13501 gatgtcctgg tgcgtggtgg cgatgtctgc cagcgcgtgg cagatttctt caaagccacg
    13561 accgaaactc tcacgcctat gaccggtcac cagaatcatc tttttatcgg gggcgataaa
    13621 cgggtaattt gccgccagtt ctgaacgcag cgtgtcgctg ctcatcacct ggtcacgcac
    13681 ccataacagt gcatcaatga ctgtattacc ggtaatgaag attcggctat ccgcaacgtt
    13741 ttcacgcagc aagttttgcc gggaagtttc ggttggagag aagtgataca tcgccagatg
    13801 cccggtcaat gtacggttag cctcttccgg ccacggcgaa tagagagcgc ccgtgcgcag
    13861 accagcctca acgtgaccaa caggaatacg ctgataaaac gccgccaggc tggttgccag
    13921 cgtcgtcgtc gtatcgccgt gaaccagcac gacgtctggt ttgaactcgg caagaatagg
    13981 ttttagccct tccagaatcc gacaggttat ctctgtcagg ccctgtcctg gctgcattat
    14041 gttgagatcg tagtcaggta caatggaaaa gagtttcagt acctgatcaa gcatctcccg
    14101 atgctgcgca gtgacgcaaa ctttagcctc aaaaaaagga tcttttgcca acgcatgcac
    14161 caacggagcc atcttgatgg cttccgggcg cgtaccaaat acagtcagta ctttcacatc
    14221 gattctcttc gaataagcgg cgagcgcctt tgcgctcacc gcagcagtgt tgctatttcg
    14281 agcaacggcg ggttaatgcg acaccagccc cgatcagccc cccgacaatg ccccacataa
    14341 tcatcaggaa ggcacgacgt gggctatcgc gttttaccgg ttcttccggc gtacgcaaat
    14401 agcgataggt ctgaaaacgc ggatccaggg ttggaccaac attcagggtg tttaacatgg
    14461 ctcgattctg atcatagtcg agatcaaagg ccggaccgac ggcctgtaaa ttttccagtc
    14521 gagcctggag cattggacgc ccaagcagga acatttctga atcaggtaat tcctcggcag
    14581 gcacatctgt cgcactgcgc gaaatattat gctgctcagc aattttcagc gcctgctcaa
    14641 tactgttcat ccggcggtcg tagatggctt tcgccacctc ttcctgacgc ttcacctgag
    14701 ctttcatctg gatggtgcgt gccgcccatg cgcctttcag ctcatcattc agatggctgg
    14761 ctgcacgctg gctggcaaaa gcaacatact gacgtaacag gttattagcg tcaggcgcgg
    14821 tttcggcaat aagcttcacg ctgtcattga ccgcgcgagt aaagtctccg gggataaact
    14881 ggatgttgtt aatcatttca tccagcaacg ccgcatcggc tttgctgttg cccaccatcc
    14941 gctgtttgta atagtcggtt tgcagccaga actctctgcg ggtatcccac gaggccagtt
    15001 gcataacaaa ctctttatag gcttcgtcca tgaccgatgg ttgatcggca gaagccatgt
    15061 ttgaacggac atccaggtta cgcaaaaatt gctgctgcga gtaatatccc cccagcatat
    15121 tcaccgttgg acgatcggta atcgccgtcg agctccactc ctgacgagca aaaaaagtat
    15181 acgccagcgc gattaacgca aacgccagcc ccatgccaat aatccatagc ttcccagccc
    15241 acaaggtacg aaacaaccca cgaatatcca gttcattttc agcgtcttcg gccggtttcc
    15301 caggcattgg ttgtgtcatc acatcctcat ttatttggtt aaattggggc tgccaccacg
    15361 atttctacgc agtctgcgtt ttacgcgctt aataaagcga gcaactttcc aggcacgctt
    15421 aatgcaatat ccatagagga agaatgctag caaaaagagc accagcatga cccactccgg
    15481 gacaaaatga gaatattctg ccagcacgcc aatggaagcg agcagtgctg cggcaagggt
    15541 aatcagcaca aacgcctgac gggaagtaaa cccggcacgc atgatcaaat ggtgaatatg
    15601 ctgacggtca ggagagaatg ggctcatgcc tttacgcagg cgacggtaca taatcgccac
    15661 catatccatt agcggaatgg cgattatcca caaagcggta accgggctga tgggatgggt
    15721 tttgccctgg gtcgtttcga gcaggatcca gataacggta aaaccaatca gcgtactgcc
    15781 cgcatcaccc ataaagactt tgtagcggcg acccaggata ccaaggttaa gcatgatgta
    15841 tggcaggatg gcggcgatca tcgcaaagca ccagattgcg aggctggttt gcccgtcgaa
    15901 ccacaaaatc ataccgattg ctgcaaacga gacgcaggac aacccgccca gcaagccatc
    15961 aatgccatca accatgttga acgcattaat ggccgcccag acggcaaata gcgtcaggaa
    16021 gtaaccaaac ggtccgagca ccatctccca ggagccaaag atataaccca ggctactgag
    16081 ataaagtttg ccgaacacca tcataacaat gccaacagcg gcctgtatgg tggcacggat
    16141 ttttacgctg atatcaaaac gatcatccag cgcgccaatg aaaacaagca caccggcaca
    16201 agcgagatag agagatgcat gcggaatata gtaatcgaca attccgaacg tgaagcaaat
    16261 ccctgcgtaa accgaaatcc ccccaacgag aggtatcaat ccctggtgac gtttgcggaa
    16321 gtttggttta tccactaaac cgactttttt tgccacctta cgggcaaaaa acagaaacag
    16381 tgtcgtgaat aaaaaaatac tgatgagatc agtactcact gtcagtaaat tcacaatgca
    16441 tgctctcaga gaaaattatt agcagaagta taaccacgaa gacctttatt cagaagggaa
    16501 agtccgttcc gaacccacca gccctgtgca aaaatcaacc atttgatatg caaattgcat
    16561 aacatgccag caaattccag gaattttcct aacatcgcca gcgcgacatt aagattacag
    16621 atataaaagc aaaacgccac gtaaacacgt ggcgtttttg gcataagaca aatttatgag
    16681 cgtttcatca tttcgaagaa atcgtcattg gtcttggtca tggccagttt attaatgagg
    16741 aattccattg catcgatttc gcccatcggg tgaatgattt tgcgcaggat ccacattttc
    16801 tgcagttctt cctgagtcgt gagcagctct tctttacggg taccggaacg gttgtagtcg
    16861 atagccggga agacgcgttt ttcagcgatc ttacgagaga ggtgcagttc catgttgcct
    16921 gtacctttaa actcttcgta aataacttcg tccattttag aaccggtatc gataagcgcc
    16981 gtcgcgataa tggtcaggct gccgccctct tccacgttac gcgccgcacc aaagaagcgt
    17041 ttcggacgat gaagggcatt ggcgtccaca ccaccggtca acactttacc tgacgccgga
    17101 acaacagtgt tgtaagcgcg cgccagacga gtgatggagt cgagcagaat gataacgtct
    17161 ttcttgtgct caaccaggcg tttggccttc tcgatcacca tttccgcaac ctgaacgtgg
    17221 cgagatgcgg gttcgtcaaa ggtagaagca acaacttcac cttttaccag acgctgcatc
    17281 tcggttactt cttccggacg ttcgtcgatc agcagaacca tcagcacaca atccgggtgg
    17341 ttgtaagcaa tgctctgagc aatgttctgc agcagcatag ttttaccggc tttcggcggt
    17401 gccacaatca gaccacgctg accacgaccg ataggtgatg ccagatccag tacgcgagca
    17461 gttaaatctt cagtagaacc gttaccacgt tccatacgca gacgagagtt tgcgtgcagc
    17521 ggggttaagt tctcaaagag gattttgttg cgggcgtttt caggtttgtc gaagttaact
    17581 tcgttaactt tcagcagcgc aaaatagcgt tcaccttctt tcggcgggcg aatcttacca
    17641 gagatggtat caccagtgcg gaggttgaaa cggcggattt ggctagggga aacgtagatg
    17701 tcatcaggac cggcgaggta ggagctgtct gcggaacgga ggaaaccaaa tccatcctgc
    17761 aatatctcca gtacgccatc accaaagata tcttcgccac tctttgcgtg ctgcttcagg
    17821 atggcaaaaa taatgtcctg cttacgcatg cgagccaggt tttccagccc catattttcg
    17881 ccgagagtga tcagctcaga aaccggcgta ttctttaatt cggtaagatt catagtggtg
    17941 tgagttctta aacttggggt aaatctcgaa cttaatgttg tgaatggtat ggcaggatca
    18001 tccatgcctg tttacggtca tcgactcatg tcttttcgct gcctggtcac aggagagtac
    18061 gcagaactga aacgacaaga cggattgagt gacgagccag aaatctgttc acttcgcatt
    18121 taagataaaa cgggaagcgt tggaaattac aagattcaaa cttaataagg tatgtttaat
    18181 acgaagtcaa cactaagtta gcatgactca cgccgggcgt ccagttttta gcgacggggc
    18241 acccgaacat gaaattccct tacgccaggt tagcgtcgag gaactctttc aactgacctt
    18301 tagacagtgc gcccactttg gttgccgcca cttcaccgtt tttgaacagc agcagagtcg
    18361 ggataccacg gatgccatat ttcggcgcag tgccagggtt ttgatcgatg ttcagttttg
    18421 caacggtcag tttgccctga tattcgtcag cgatttcatc cagaatcggg gcgatcattt
    18481 tgcacggacc gcaccactct gcccagaaat cgacgaggat cgccccgtcc gctttgagta
    18541 catccgtgtc aaaactgtcg tcagtcaggt gaataatttt atcgctcata tataactcca
    18601 caggaataag cctggcgtgt tggtttcgtt gttggtgtaa cattaaccaa ctaaaggttg
    18661 actttatttc accggatacg ctttcgtaaa gcaatagtaa gctgatattc taccacacta
    18721 tgagcaaaac acatttaaca gaacagaagt tttccgactt cgccctgcat ccgaaggttg
    18781 tagaagccct tgaaaaaaaa gggtttcata actgtacgcc cattcaggca ctggcccttc
    18841 cgctgacgct ggcgggtcgt gacgtagccg ggcaggcgca aaccggtacc gggaaaacga
    18901 tggcgtttct tacgtcaacg tttcattatc ttctctctca tcctgcgatt gccgatcgca
    18961 aggtgaatca accgcgtgcc ttaattatgg caccgacgcg tgaacttgcc gtgcagatcc
    19021 acgccgacgc ggaaccgctg gcggaagcta ccggcctgaa gctgggcctg gcttacggtg
    19081 gtgatggcta tgacaaacag ctgaaagtgc tggaaagcgg cgttgacatt ctgattggca
    19141 ccacgggtcg tttaattgac tacgccaagc agaaccacat taacctcggt gccattcagg
    19201 tagtggtact ggacgaagcc gatcgcatgt acgatctagg ctttattaaa gatatccgct
    19261 ggctgttccg ccgtatgccg cctgcaaatc agcgcctcaa catgctgttc tccgccacgc
    19321 tttcgtaccg ggtacgtgaa ctggcgttcg agcagatgaa caatgccgaa tatattgaag
    19381 tggaaccgga acagaaaacg ggccaccgta ttaaagaaga gcttttctac ccttctaacg
    19441 aagaaaaaat gcgtttgctg caaacgctga tcgaagaaga gtggccagac agagcgatta
    19501 ttttcgccaa taccaaacac cgttgtgaag agatctgggg ccacctggcg gcagatggtc
    19561 atcgtgtcgg tttgttgaca ggcgatgtcg cgcagaaaaa acgtctgcgt attcttgatg
    19621 aatttacccg tggcgatctg gatattctgg ttgccaccga cgttgccgcg cgtggtttgc
    19681 atattccggc agtgacgcac gtctttaact acgatttacc cgatgactgt gaagattacg
    19741 ttcaccgtat tggtcgtaca ggtcgcgcag gcgcaagcgg tcactctatc agcctggcgt
    19801 gtgaagagta tgcattgaat ttgcctgcta ttgagaccta tattggtcac tcaattccgg
    19861 taagcaaata caatccggac gcattgatga ccgatctgcc aaaaccgctg cgcctcacgc
    19921 gcccgcgcac aggcaatggt ccgcgtcgta ctggcgctcc gcgtaatcgt cgtcgttcag
    19981 gttaaataaa atgtcatact caaaatcatt caaactgtat caagatggca agagaatgaa
    20041 tcccgatgag cttactgtag taagtgattc gggtgaaaga atgcagccaa cacagagaca
    20101 gattgaagga tgaagagtat gggttccacc tcgtcgctgt atgcagccat tgatctcggt
    20161 tcgaatagtt ttcatatgct ggttgtgcgc gaggtggctg gaagcatcca gacgctgacg
    20221 cgaattaaac gcaaagtgcg tctggctgct ggcctgaata gcgaaaatga cctctctaat
    20281 gaagcaatgg agcgcggttg gcaatgtctg cgcctgtttg ctgaacgtct gcaagatatc
    20341 cctccctcgc aaattcgcgt tgtcgctacg gcgacattac gacttgccgt caatgcgggt
    20401 gattttattg ccaaagcgca ggaaatcctc ggttgtccgg tacaggtgat cagcggtgaa
    20461 gaggaagcgc gtctgattta tcagggcgtt gctcacacca ctggtggtgc cgatcagcgc
    20521 ctggtggtgg atataggcgg tgccagtact gaactggtaa ccggcacggg tgcacaaacc
    20581 acctcgttgt tcagcctgtc gatgggctgc gtcacctggc tggaacgcta ttttgccgat
    20641 cgtaatctgg ggcaagaaaa ttttgatgct gcagaaaaag cggcacgcga agtgttacgt
    20701 ccggttgccg atgaattacg gtatcacggc tggaaagtgt gcgttggtgc ttccggcacc
    20761 gtgcaggcgt tacaggaaat catgatggca caggggatgg atgaacgcat taccctggaa
    20821 aagttgcagc aattgaaaca gcgagccatt cattgcggtc ggctggagga actggagatt
    20881 gacgggctga cgctggaacg tgcattagtg ttcccgagtg gtctggcgat cctgatcgcc
    20941 atttttaccg aactgaatat tcagtgtatg accctggcgg gcggtgcgct gcgtgaaggc
    21001 ctggtctacg gcatgttgca tctggccgtc gagcaggata ttcgcagccg tacactgcgt
    21061 aatattcaac gccgctttat gatcgatatt gatcaggcac agcgcgtagc caaagttgcg
    21121 gctaacttct tcgatcaggt ggaaaacgaa tggcatcttg aagcaataag ccgcgatttg
    21181 ctcatcagcg cctgtcagct tcatgaaatc ggcctgagcg ttgacttcaa acaagctccg
    21241 caacacgctg cttatctggt gcgtaacctg gatcttcccg gttttacccc cgcacagaag
    21301 aaactgctgg cgacgctact gctcaaccag actaacccgg tcgatctctc atcgctgcat
    21361 cagcaaaatg ccgtaccacc gcgcgtcgca gaacaactct gccgtttact ccgcctggct
    21421 atcatttttg ccagccgtcg ccgtgacgat ctcgtgccag agatgacatt acaggctaac
    21481 catgaactgt tgaccttgac gcttccgcaa ggttggctaa cccaacatcc gctgggtaaa
    21541 gagattattg ctcaggaaaa ccagtggcag agctatgtcc actggccgct ggaagtgcat
    21601 taatgagtaa gtgccggatg cgatgctgac gcatcttctc cggccttgat tatttccctc
    21661 gttttgccgc catcatcgct ttcagattcg ccagatggct ttgccctttc tgcatccgtt
    21721 cttcggcact gaccactttg cgctcctgtt cccaaatcag atcatcctgc ggcagctcca
    21781 gcaaaaagcg gctcggctcc gggcgcacca gttcgccgta ctgacggcgt tctttacaca
    21841 gcgtaaaggt caattccttc tgggcgcggg taatgccgac ataggccagc cgccgctcct
    21901 catcgatatt atcttcatcg atactgctct ggtgcggcaa aaacccttct tccataccga
    21961 ccatgtagac ataaggaaac tccagccctt ttgacgcgtg gagagtcatc agttgcacct
    22021 gatccagctc ttcttcactc tcaccacgct ccatcatgtc gcgtaaagta aagcgcgtca
    22081 ccacctgggt gagcgtcatc ggctcatcca gttcgctgcc ttccagcatc tccgtcatcc
    22141 agctaaacag ttggttgacg ttcttcatgc gcatttcggc agctttcgtg ctgggcgatg
    22201 tttcgtacag ccaggattca taatccatgc cgtggatcag atcgcgcacc gcggcaatcg
    22261 gctcccgctc cgccagacgc tggatttctg ccaaccagtg agtgaagcgg gtcaatgctt
    22321 cataaccacg tccgctaagc gtctgactca ggcccatatc aaagctggcg gtaaacatgc
    22381 ttttattgcg cgtcatcgcc cactcaccca gctttttcag cgtcgccggg ccaatctctc
    22441 gcttcggcgt gttaacgata cgcagaaatg cgctgtcatc gtccgggtta gtcagcacgc
    22501 gcagataagc cagcaagtcc ttgatttcag gacgagagaa aaacgacgta ccaccagaaa
    22561 ttttgtacgg gatgcggttt tgcatcagga atttttcaaa cacccgcgac tgatagttac
    22621 cgcgataaag aatagcgtaa tctttgtact gcgttttatt gacgaagtga tgcgcaatca
    22681 gttcaccagt gacgcgctcg gcttcatgtt cttcgttatt cgcgcttaaa acttttagct
    22741 ctgtgccgta acccagttcg gagaacagac gcttttcaaa gacgtgcggg ttattggcga
    22801 tcaggatgtt cgccgctttc aaaatacgtc cggaagagcg gtagttctgc tcaagcttaa
    22861 tcaccttcag cgccggaaaa tcctggctca gcagcaccag gttttgcgga cgtgcgccgc
    22921 gccaggagta gatcgactgg tcatcgtcac ccaccacggt aaagcgcgcg cggctgccca
    22981 ccagcagttt caccagttca tactggctgg tgttggtgtc ctgatattcg tccaccagca
    23041 ggtagcgaat tttgttctgc cagcgctcgc ggacttcttc attgcgttgc agaagtagcg
    23101 tcggcaataa aatcagatca tcgaagtcga gcacattaca ggctttcagg tgtgcatcat
    23161 acagcccata acaatgggca aaaatacggt cccgctcgcc aatcgcactg gctgccgcct
    23221 gggacggtgt tttgagatcg ttcttccagt tagagatggt cgaaatcaac tgttgcagga
    23281 gaactttgtc atcttcaatc agcccttcgg tcaactcttt gagcaacgcc agctgatcgg
    23341 tatcgtcaaa cagcgaaaag ttcgccttca tccccagcgc cgcatactca cgcttgatga
    23401 tatccagccc cagcgtgtgg aaggttgaga tcatcagccc atgcgcctct ttgcgaccca
    23461 gcgtctgtcc gacacgctct ttcatctcgc gcgccgcctt attggtaaag gtcaccgccg
    23521 cgatatgtcg cgcctgataa ccgcacccgc ggatcagatg ggcgatttta ttggtgataa
    23581 cacgagtttt accggaaccc gcacccgcca gcaccaggca ggggccggta acgaattcga
    23641 cagcttgttg ttggccgggg tttagacgca taggaaaatc gctcaatgaa agtcgggagg
    23701 ggaaaataac agcgtggtag tatagccagc ctcatcctta ccactcaagg cacgatcatg
    23761 gcaaaaacag cagcagcact gcatatcctt gtaaaagaag agaaactggc tctggatctt
    23821 ctcgtgcaga ttaagaacgg tgccgatttc ggcaagctgg cgaagaaaca ctccatttgc
    23881 ccatcaggca aacgcggcgg tgatttaggt gaattccgcc agggtcagat ggttccggcg
    23941 ttcgataaag ttgtgttctc ttgtccggta ctggagccaa ccggcccgct gcacacccag
    24001 ttcggttacc acatcattaa ggtgctgtac cgtaactaat agcaaggcct tctccgggag
    24061 aaggccttga actctgaatc gctattaacc cgcaacagca atacgtttca tatccgtcat
    24121 atagccgcgc agtttcttac ctacttgctc aatcgcatgg ctgcgaatcg cttcatttac
    24181 atcacgcagc tgcgcgttat ctaccgcacc ttccggaata gctttaccca aatcgcccgg
    24241 ttgcagttct gccataaacg gtttcagcag cggcacacaa gcgtaagaga acagatagtt
    24301 accgtactcg gcagtatcag agataaccac gttcatttca tacagacgct tacggacgat
    24361 ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt cttcgatgat
    24421 gccggaatca accatggttt cgaacgccag ttcaacgccc gctttcacca tcgcgatcat
    24481 cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact gcggcgcggt
    24541 ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat catcgttggc
    24601 ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca tatgtttctg
    24661 gaacagcggt gccatgatct ctttcagctg ttcagacaac gcgtaagcac gcagtttcgc
    24721 cgggttagag agacggtcca tcatcagtgt gataccgccc tgcttcagcg cttcggtgat
    24781 ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc cctcttccac
    24841 cagcttgtcg aagcacagca gcgagccagc ctgcaacata ccgcacagga tggtttgctc
    24901 acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc ccgcacggtg
    24961 accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt tcggatcgtt
    25021 ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact cttcacgcac
    25081 ttcggtgcca gggcatttcg gcgcaaccat gactacggtg atgtctttac ggatctgctc
    25141 gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt ctttcatcag
    25201 tggctgtacg gtgcgcacta catcagagtg ctgcttgtcc ggcgtcaggt taaccaccag
    25261 atccgcctgt gggatcagtt cttcgtaagt acccacttta aaaccatttt cggtcgcttt
    25321 acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg agatatcgag
    25381 accagaatca cgcatgttca gaccctggtt cagaccttgt gcgccacagc cgacgatgac
    25441 tactttttta ccctgaaggt agctcgcgcc atcggcgaat tcatcgcgcc ccataaagcg
    25501 acatttgccc agctgtgcca gctgctgacg cagattcagt gtattgaagt agttagccat
    25561 ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga gattgaattc
    25621 actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac attgcaattt
    25681 ctgcaacgtc aacattgagg gctgcgccat tggatttacg cgatctgaaa accttcctgc
    25741 atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt agcccgtcca
    25801 cgctctcacg gcagattcag cgcctggaag aagatctcgg ccagccgctg tttgtgcgcg
    25861 ataaccgcac ggtgacgctg actgaagcgg gcgaagaact gcgcgttttc gcccagcaaa
    25921 cgctgttgca gtatcagcag ctgcgccaca ccatcgacca gcaagggccg ttgctctatg
    25981 gcgaattaca tatcttctgc tcggtgaccg ccgcttacag ccatctgccg ccgattctgg
    26041 atcgcttccg cgcggaacac ccgtcggtgg agatcaaact cactaccggt gatgcggcag
    26101 atgcaatgga aaaagtcgtc acaggtgaag ctgatctggc gattgccggg aagccagaga
    26161 ctctgcccgg cgcggtggcg ttttcgatgt tggagaatct ggcagtggtg ttgattgcac
    26221 ccgcattgcc ctgcccggtg cgtaatcagg tttctgtcga aaagccggac tggtcaacgg
    26281 taccgtttat catggccaat caggggccgg tgcgccgccg cattgagttg tggttccgac
    26341 gcaataaaat cagtaacccg atgatttacg ccacggttgg cgggcatgaa gcgatggtat
    26401 cgatggtggc gctcggctgt ggcgtggcat tgttgccgga agtggtgctg gaaaacagcc
    26461 ccgaaccggt gcgtaaccgc gtgatgattt tagagcgcag cgatgagaaa acgccgtttg
    26521 agctgggcgt gtgtgcgcag aaaaagcggc tacatgagcc gctaattgag gcattctgga
    26581 agattttgcc gaaccattaa cccgccaaaa agaacctgaa cgccgggtta ttggtttcgt
    26641 cgtggcaatc gtagcccagc tcattcagcc gggtttcgaa atccggttca tggtcgccaa
    26701 gttcgaacgc cgccagtacg cgcccgtagt cggtgccatg gctgcgatag tggaacaaag
    26761 aaatgttcca gtgcgtaccc agcgtgttga ggaagcgcag cagcgcgccc ggtgattccg
    26821 ggaattcgaa gctgtagagg cgttcctgca acggatgtga cggacgcccg ccgaccatat
    26881 agcgcacatg caacttcgcc atttcgtcgt cggagagatc caccacgctg tagccgccgt
    26941 cgttgagcat ctgcaaaatt tctttgcgct cttcgaggcc gcggctcagg cgcacgccga
    27001 caaagatgca ggcgtttttg gcatcggcaa aacggtagtt gaactcggtg accgatcgcc
    27061 cgccaagcag ttggcagaat ttgaggaagc tgcctttttc ttccggaatg gtcaccgcca
    27121 acaacgcttc acgctgttcg cccagttcgc agcgttccga gacgtagcgc aggccgtgga
    27181 agttcacgtt ggcaccggaa agaatatgcg ccagccgttc gccgcgaatg ttgtgctggg
    27241 cgatatattt tttcattccc gccagcgcca gcgcgccaga gggttccgcc accgcacgca
    27301 catcttcgaa taaatccttc atcgccgcac agatcgcatc gctatcgacg gtgatgatgt
    27361 cgtcgagata ctcctggcat aaacggaagg tttcgtcacc gatgcgtttt accgcgacac
    27421 cttcggcgaa cagtcccacg cgtggcagat caaccggatg acccgcatcc agcgctgctt
    27481 tcaggcaggc ggagtcttcc gcttctacgg cgatcacttt gatttgcggc atcagttgtt
    27541 tgatcagcac cgctacgccc gccgccagac cgccgccgcc gactggcaca aatacgcggt
    27601 cgagatgggc gtcctgctgg agcagttcca gcgccagcgt gccttgcccg gcaatcacca
    27661 tcggatggtc gaacggcggc acccaggtga acccctgctg ctgtgacagt tcgatcgctt
    27721 tagctttcgc ttcgtcaaag ttcgcgccat gaagcagcac ttcgccgccg aatccacgta
    27781 ccgcatcgac tttgatgtcg gcggtggcgg ttggcataac gatcagggcc ttcacgccta
    27841 accgcgcaga agaaaacgcg acgccctgcg cgtggttacc cgcagaagca gtgatcacgc
    27901 cgtgcgcttt ttgttcttcc gtcaggcccg ccatcatggc gtatgcgccg cgcagcttaa
    27961 agctgtgcac tggctggcga tcttcgcgct tcaccagaat gacgttatca agacgcgacg
    28021 acagtttttc cattttttgt agcggcgtaa cctgcgccgc ctcgtaaacc ggcgcgcgca
    28081 gcactgctct taaatattcg gcaccttccg gagcaccgga caggggttgc gagtcagcca
    28141 ttattaaccc cccagtttcg atttatcgcg caccgcacct ttgtcggcgc tggttgccag
    28201 gctggcgtag gcgcacaggg caaaggaaac ctgacgttca cggtttttcg gcgtccaggc
    28261 tttgtcaccc cgagcttcct gcgcttcacg acgcgccgcc agttcggcat cgcttacctg
    28321 taactgaatg ccacggttcg ggatgtcgat agcgatcagg tcaccatctt caatcagacc
    28381 aatgctgccg ccgcttgccg cttccggtga gacgtggccg atggaaagac cagaggtgcc
    28441 gccagagaaa cgaccgtcgg tgatcagcgc acaggcttta ccgagaccca ttgatttcag
    28501 gaagctggtt gggtagagca tttcctgcat gccagggccg cctttcgggc cttcatagcg
    28561 gatcaccacc acatcgcccg cgacaacttt gccaccgagg atcgcttcta ccgcgtcgtc
    28621 ctggctttcg tacactttcg ccgggccggt gaatttgagg atgctgtcat cgacgcccgc
    28681 ggttttcacg atgcagccgt tttcagcaaa attaccgtag agcaccgcca ggccgccgtc
    28741 tttgctgtag gcgtgttcca gcgagcggat acagccgttt gcgcgatcgt catcgagaga
    28801 atcccaacgg caatcctgcg agaatgcctg tgtagtacga atgcccgcag ggcctgcgcg
    28861 gaacatattt tttaccgcgt catcctgggt cagcataacg tcgtattgtt ccagagtttg
    28921 cggcaacgtc aggccaagta cgtttttcac atcacggttc agtaaccccg cgcgatccag
    28981 ttcaccgaga ataccgataa caccaccagc acggtgaaca tcttccatat ggtatttttg
    29041 ggtgctcggc gcaactttgc ataactgcgg gactttacgc gaaagtttgt cgatatcact
    29101 catggtgaag tcgatttccg cttcctgcgc cgccgccagc aggtgaagta ccgtgttggt
    29161 agagccgccc atcgcgatat ccagcgtcat ggcattttca aacgccgctt tgctagcaat
    29221 attgcgcggc agtgcacttt cgtcgttttg ctcgtagtaa cgtttggtca attcaacaat
    29281 gcgtttaccg gcattgagga acaactgctt acggtccgcg tgggttgcca gcagcgagcc
    29341 gttgcccggc tgcgacaggc ccagcgcttc ggtcaggcag ttcattgagt tagcggtaaa
    29401 catcccggag caggagccgc aggtcggaca cgcggaacgt tcaacctgat cgctctggga
    29461 gtcagaaact ttcgggtctg cgccctggat catcgcatca accagatcga gtttgattat
    29521 ctgatcggac agtttggttt tcccggcctc catcgggccg ccggaaacaa agatcaccgg
    29581 aatattcagg cgcagggaag ccatcagcat ccccggggtg attttgtcgc agttagagat
    29641 gcagaccatg gcgtcggcgc agtgggcgtt gaccatatac tcaacggaat cagcgatcag
    29701 ttcgcgagat ggaagtgaat aaagcatccc cccgtggccc atggcaatcc catcatccac
    29761 cgcaatggtg ttgaactctt tggcaacgcc gccagccgct tcaatttgtt cggcgaccag
    29821 tttaccgaga tcgcgcagat ggacgtgacc cggtacaaat tgggtgaacg agttcacaac
    29881 cgcgataatc ggcttgccaa aatcggcgtc ggtcattccg gtggcgcgcc acagcgcacg
    29941 agcacccgcc atattacgac catgagtggt ggtggcggaa cggtacttag gcatacttta
    30001 tttactccca gtgtctgtct cgtaaatggg acggtgcgtg ccgtcccatt ttttgtattt
    30061 attgattaac ttgatctaac cagccccatt tatcttcggt ttcgccagtg aagaggccga
    30121 agaaggcttg ctgaatgcgt ttggtaaccg ggccacaacg gccttcgcca atctgaatac
    30181 cgtctacgct gcgcactggc gtgatttctg ccgccgtacc ggacataaag acttcatccg
    30241 ccaagtacag ggattcgcgc gacagcacct gctcacgcac ttcaattccc agctctttcg
    30301 ccagtttgat gatggcatca cgggtaatac ccggcagcgc ggaggaggtg aacggtgggg
    30361 tgaacagcac accatctttc acttcaaaca ggttttcgcc tgcgccttca gagatataac
    30421 cgttcacatc cagcgcgata ccttcctgat aaccgtggcg gcgcgcttcg ctacccacca
    30481 gcagggaaga gagatagtta ccaccggctt ttgccgcagt tgggatggtg tttggtgctg
    30541 cgcggttcca ggaggaaacc atcgcatcga tcccctgctc cagcgcttct gcgcccagat
    30601 acgctcccca cgggaaagcg gcgataatca catcggttga gtatcccgct ggcgggttta
    30661 ctcccatgcc aacatcaccg acgaagatca gcggacggat ataggcgctg gtgagattgt
    30721 ttttgcggat cacgtcacga caagcttcca tcagctcatc aatgctctgt gaaaccggga
    30781 agcgatagat tttggcggag tcatgcagac gctgcatatg ctcacgatgg cggaatacaa
    30841 ccggtccttt gtgtgagtcg tagcaacgga tgccttcaaa aaccgaggtg ccatagtgca
    30901 gcgcgtgcga catcacatgc accttcgcgt cttcccagcg aaccatctcc ccattgaacc
    30961 aaatgtaatc agctttcttc gtggtcattt ttatattcct tttgcgctca ggcgcggatt
    31021 tgttgtgatg tggttgtgct ctggcagatg gcaacgtgtg cgacgtccac cagtttattt
    31081 aactgactaa acagtaagtc gaccgaccgt gggctggcaa cggtcaattc gatatttata
    31141 ttttgtgcat cgctggcggc agccatattc attgagcaga cgtggaaacc acgatgacgc
    31201 accacgcgta aaacacgttc taaggtttcc ggattgaagc gagccgatac attgacctga
    31261 tgttgcatca tgataatttc tccaacattt ctgaattact ggcacctggc ggcaccagcg
    31321 gccagacgtt ctcaagttcg tcgattgaga catgaagcag gtatggccca tcactgttca
    31381 gcatggtgtc gagtgccgct tcaacctggt ctttacgggt gatgtgttgg ccagggatgc
    31441 cgaaggcgct ggctaacatg aggaaatcgg ggttatcggt aagggtggtt tcgctgtatc
    31501 gttcctgaaa aaacagttgc tgccattgtc gaaccatccc taaccgttgg ttatcgagta
    31561 agacgatttt caacggtaac tgcttgcgtt ttacggtgcc cagctcttgc acattcatca
    31621 tgaaagagcc gtcaccggag atacagacaa cggtatcgtt cggtcgcgcg acttgtgcgc
    31681 caactgccgc cggtaaacca aaacccatgg tacctaagcc gctggaggtg atgaaatttt
    31741 ccgggcgagt gtgggcgatg tgctgcgcag cccacatctg gtgctgcccc acatctgtgg
    31801 tcacgacgca atccgcaggt ttacgatccg acagttgttt taacaacaac ggagcgtaga
    31861 tagcgtcacc gggatggtcg taacgccagg aatgttcatc acgcagctgc gcgcagtgtt
    31921 gctgccagtc attgatattt aacggctgct gtaatgctgg taacagagca tttaaatcac
    31981 cttgtaatgc cacatgtgcc tgacgcagct tgttcatttc tgccgggtcg atatccatat
    32041 ggataacact ggcgtgtggt gcgaaggtgt tcagtttgcc ggtcacccgg tcatcaaaac
    32101 gtgcacccac ggcgatcagc aggtcacact cctgcaccgc gaagtttgcc gctttggttc
    32161 cgtgcatccc cagcatgccc agatagtacg gataatctgc ttctactgcg cccagccctt
    32221 tcagcgtaca ggtggcaggc atttttgtgg cagcgagaaa ttcacgcaaa gccggaactg
    32281 cctgcgccat ccccacgcca ccgccaacgt acagcatcgg tttttgcgct tttgccagca
    32341 tctggcgcgc ttgctcgact tcggcatgtg ggaaagtcac ttcgttttca acggtggtga
    32401 accacggttc cagatcgccg ctggctaact ggatatcttt tgggatatcg accagaaccg
    32461 gaccaggacg acctgagctg gcaacgtcga atgcttcagc catgatgcgc ggcaactctt
    32521 ccagagactg caccagaaag ctgtgcttgg tacaggctaa cgacaatccc aggacatcca
    32581 cttcctgaaa tgcgtcggtg ccgataaacg gtgcggacac ctgaccagtg atggcaacga
    32641 cagggatgga atctaacagt gcgtccgcaa gcccggttat caggttggtt gcgcccggac
    32701 cagacgtggc gatacatacg ccagttttac cggtagcacg ggcataaccg atagccgcca
    32761 ttgccgcacc ctgctcatgt cggcacagca agtgctccac gccgccgtca tacaatgcat
    32821 cgtaaaccgg cataattgcg ccacccggat aaccgaaaac ggtgttcaca ccctgtgccc
    32881 gcaacgcatg taccacccac tgtgcgccat tcatagttag ttccccgtcc tgaatcttga
    32941 gaaacagaat tttgtgctgt tattcattgt ctgctcctcg gttatgtttt taaggtcaaa
    33001 aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat ctctaagcct
    33061 ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg ctaatcacga
    33121 ccaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct tgttcgaagg
    33181 aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttg ggcctatttt
    33241 tatgatggct ttaataattt caatagaaat tgttgttttt taaaaagaaa atagtgagtg
    33301 aaaaaattca atttcagtat aaatgttgtt cccttcctcg tagttcatcc tttgtccgct
    33361 gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc acaaataaac
    33421 aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc ttatgcacat
    33481 aatggtgact caaggagggt ttatgtcact gtcaattgtt catacccgcg cagccctgga
    33541 agtaaatgcg cccccaatca ctgttgaggt acatatcagt aaaggtctac ccggcttaac
    33601 gatggtgggc ttaacagaaa caacggtgaa agaagctcgc gatcgcgtgc gcagcgccat
    33661 tatcaatagc ggatatgaat atccggcgaa aaaaatcacc atcaaccttg ctccagccga
    33721 tctgccaaaa gaagggggac gatatgattt acctatcgcc attgcgttgc tggcggcctc
    33781 agaacagctt acagccaata agttagatga atatgaatta gtcggagaac tagcgcttac
    33841 aggcgctctg cgtggcgttc ccggtgcaat ctccagtgca actgaagcta ttaagtcggg
    33901 cagaaaaatt atcgtcgcga aagataacga agatgaagtg gggctaatta acggtgaagg
    33961 atgcctgata gccgatcatc tgcaggctgt ctgtgcgttt ctggaaggta agaacactct
    34021 cgaacgcccg aaaccaactg atgcagtatc ccgggcgcta caacatgatc tcagtgatgt
    34081 tgtcggtcag gagcaaggaa aacgaggact ggaaattacc gccgctggtg ggcacaacct
    34141 tttactgatt gggccgccgg gaacaggtaa aacaatgctc gccagccgta ttaatggcct
    34201 gttgccagat ttaagcaatg aagaggcact ggagagcgct gcgatattaa gtctggtaaa
    34261 tgctgaatca gtacaaaaac aatggcggca gcgcccgttc cgctcacctc atcacagtgc
    34321 gtcgttaact gcgatggtgg gcggtggtgc aattccaggg ccaggtgaaa tttcgttggc
    34381 gcataacggc gtgctttttc ttgatgagct acctgaattt gaacggcgta cactggatgc
    34441 cttgcgagag ccgattgaat ccgggcagat ccatctttca cgcacacgag caaaaataac
    34501 ctatccagcc cgtttccagc ttgttgcggc gatgaatccc agccctaccg gacattatca
    34561 gggaaaccat aaccgctgca cgccagaaca gacattacgt tatctcactg gagatcccat
    34621 taccaccccc cggcattttg agtaaaacgg tagtgccggg agaaagcagc gccaccgtta
    34681 aacaacgcgt aatggccgcc agagagcgcc aatttaagcg gcagaataaa ctgaatgcct
    34741 ggctggatag tccggaaata cgccaattct gcaaacttga gagcgaagat gcgatgtggt
    34801 tggaagaaac gctgatccat ctggggttat cgattcgtgc ctggcagcgg ttactgaaag
    34861 ttgcacgaac cattgctgat attgatcagt ctgacattat cacacgtcag catttgcagg
    34921 aggcagttag ctatcgtgcg attgaccgtt tgctcatcca tctgcaaaaa ctactgacat
    34981 aaaaaaaggg catttcgccc tttttattaa tcgtcagaat cggtgtagtc ttcagcacct
    35041 tcaacctgcg gtttaccgcc ggaaagggtg tgaaaacgtt ttggacgctt aatacgcgtc
    35101 atatacttgg accacacgcg ttctgcttct gtcactggct cacgttcgcc acggcatact
    35161 gctacgaaga gtttctcttc ctcggtaacc ggctcgcgtt tgccaagatc caactcattg
    35221 aaggcataac catgacgctc aagcagttgt gcctctttga tggtgaaatc accatgacga
    35281 gagaatccac gtggataatg tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc
    35341 gccatcctgc acgctcctaa ttctttgacc gagctagtta tggcgcggag tattagttac
    35401 gcttgacaga gtgtaaaaca aaacatttaa atcataacga caaataattt tgcggagagc
    35461 actgtggata cggaattgtt aaaaactttc ctggaagtta gccgaacgcg tcactttggt
    35521 cgagcggctg aatcgctcta tctgacccag tcagcagtga gctttcgaat cagacaactg
    35581 gaaaatcaac tgggtgtgaa ccttttcacc cgccacagaa acaatatccg tttaaccgct
    35641 gccggtgaaa aactactgcc ttatgcagaa acgctcatga gcacctggca ggccgcccgc
    35701 aaggaggtgg cgcatacctc acgacataac gagttttcta tcggtgccag cgcctcgttg
    35761 tgggaatgta tgcttaatca gtggttggga cgcttgtatc aaaatcagga tgcccataca
    35821 ggcttacagt tcgaagcgcg aattgcccaa cggcagtctc tggtaaaaca gctgcatgaa
    35881 cgccagcttg atcttcttat taccactgaa gcgcccaaaa tggacgaatt tagtagtcag
    35941 ttgctggggt atttcacttt agcgctttat accagtgccc cttctaaact aaagggagat
    36001 cttaactatc tgcgactgga gtgggggcca gattttcaac agcatgaggc aggtttgatc
    36061 ggtgctgacg aagttcccat tctgacaacc agttctgctg aactggcaca gcaacagatt
    36121 gcgatgctta atggttgcac ctggctaccc gtcagctggg cgcgtaaaaa aggcggcctg
    36181 cataccgttg tcgatagcac aacactttca cggccgcttt atgccatatg gctgcaaaat
    36241 agcgataaaa atgcgttgat tcgcgatctt ttgaaaatta acgtgctgga tgaagtgtat
    36301 taatctgaat ggctggcaag gatgccggta gaaggattta cttcggagag gattatttca
    36361 gataaaaaaa atccttagct ttcgctaagg atgatttctg gcaggggcgg agagactcga
    36421 actcccaaca cccggttttg gagaccggtg ctctaccaat tgaactacgc ccctaattag
    36481 ggtggcggaa cggacgggac tcgaacccgc gaccccctgc gtgacaggca ggtattctaa
    36541 ccgactgaac taccgctcca ccgaattctt ttacaaccac cggtttta
//
LOCUS       DAJTUI010000032        35789 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTUI010000032 DAJTUI010000000
VERSION     DAJTUI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.032"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..245
                     /locus_tag="OO464_001742"
     CDS             <1..245
                     /locus_tag="OO464_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6226916.1"
                     /translation="TIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSIE
                     QRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(292..1050)
                     /gene="deoR"
                     /locus_tag="OO464_001743"
     CDS             complement(292..1050)
                     /gene="deoR"
                     /locus_tag="OO464_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6226917.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1108..1704)
                     /gene="ybjG"
                     /locus_tag="OO464_001744"
     CDS             complement(1108..1704)
                     /gene="ybjG"
                     /locus_tag="OO464_001744"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6226918.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1989..3215
                     /gene="mdfA"
                     /locus_tag="OO464_001745"
     CDS             1989..3215
                     /gene="mdfA"
                     /locus_tag="OO464_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR6226919.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3256..3540)
                     /locus_tag="OO464_001746"
     CDS             complement(3256..3540)
                     /locus_tag="OO464_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226920.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3626..4441)
                     /gene="ybjI"
                     /locus_tag="OO464_001747"
     CDS             complement(3626..4441)
                     /gene="ybjI"
                     /locus_tag="OO464_001747"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6226921.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4441..5649)
                     /locus_tag="OO464_001748"
     CDS             complement(4441..5649)
                     /locus_tag="OO464_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6226922.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5733..6269
                     /gene="rcdA"
                     /locus_tag="OO464_001749"
     CDS             5733..6269
                     /gene="rcdA"
                     /locus_tag="OO464_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6226923.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6444..8129)
                     /locus_tag="OO464_001750"
     CDS             complement(6444..8129)
                     /locus_tag="OO464_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6226924.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8399..8776
                     /locus_tag="OO464_001751"
     CDS             8399..8776
                     /locus_tag="OO464_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6226925.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8806..9063)
                     /gene="grxA"
                     /locus_tag="OO464_001752"
     CDS             complement(8806..9063)
                     /gene="grxA"
                     /locus_tag="OO464_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6226926.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9223..9510
                     /locus_tag="OO464_001753"
     CDS             9223..9510
                     /locus_tag="OO464_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6226927.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9494..10216
                     /gene="nfsA"
                     /locus_tag="OO464_001754"
     CDS             9494..10216
                     /gene="nfsA"
                     /locus_tag="OO464_001754"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR6226928.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10277..11179
                     /gene="rimK"
                     /locus_tag="OO464_001755"
     CDS             10277..11179
                     /gene="rimK"
                     /locus_tag="OO464_001755"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6226929.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11267..11743
                     /locus_tag="OO464_001756"
     CDS             11267..11743
                     /locus_tag="OO464_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6226930.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12095..13207
                     /gene="potF"
                     /locus_tag="OO464_001757"
     CDS             12095..13207
                     /gene="potF"
                     /locus_tag="OO464_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6226931.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVHENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13302..14435
                     /gene="potG"
                     /locus_tag="OO464_001758"
     CDS             13302..14435
                     /gene="potG"
                     /locus_tag="OO464_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6226932.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14445..15398
                     /gene="potH"
                     /locus_tag="OO464_001759"
     CDS             14445..15398
                     /gene="potH"
                     /locus_tag="OO464_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR6226933.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15395..16241
                     /gene="potI"
                     /locus_tag="OO464_001760"
                     /pseudo
     CDS             15395..16241
                     /gene="potI"
                     /locus_tag="OO464_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
     gene            16301..16789
                     /locus_tag="OO464_001761"
     CDS             16301..16789
                     /locus_tag="OO464_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6226934.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16830..17957
                     /gene="rlmC"
                     /locus_tag="OO464_001762"
     CDS             16830..17957
                     /gene="rlmC"
                     /locus_tag="OO464_001762"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6226935.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="OO464_001763"
     CDS             complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="OO464_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6226936.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19155..19823)
                     /gene="artM"
                     /locus_tag="OO464_001764"
     CDS             complement(19155..19823)
                     /gene="artM"
                     /locus_tag="OO464_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6226937.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="OO464_001765"
     CDS             complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="OO464_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6226938.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20546..21277)
                     /gene="artJ"
                     /locus_tag="OO464_001766"
     CDS             complement(20546..21277)
                     /gene="artJ"
                     /locus_tag="OO464_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6226939.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21295..22023)
                     /gene="artP"
                     /locus_tag="OO464_001767"
     CDS             complement(21295..22023)
                     /gene="artP"
                     /locus_tag="OO464_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6226940.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22241..22756)
                     /locus_tag="OO464_001768"
     CDS             complement(22241..22756)
                     /locus_tag="OO464_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6226941.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23405..25174
                     /locus_tag="OO464_001769"
     CDS             23405..25174
                     /locus_tag="OO464_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6226942.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25385..25708
                     /locus_tag="OO464_001770"
     CDS             25385..25708
                     /locus_tag="OO464_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6226943.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25705..26535
                     /gene="amiD"
                     /locus_tag="OO464_001771"
     CDS             25705..26535
                     /gene="amiD"
                     /locus_tag="OO464_001771"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6226944.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26532..27545)
                     /locus_tag="OO464_001772"
     CDS             complement(26532..27545)
                     /locus_tag="OO464_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6226945.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27644..29074)
                     /locus_tag="OO464_001773"
     CDS             complement(27644..29074)
                     /locus_tag="OO464_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6226946.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29085..30086)
                     /gene="ltaE"
                     /locus_tag="OO464_001774"
     CDS             complement(29085..30086)
                     /gene="ltaE"
                     /locus_tag="OO464_001774"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6226947.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30123..31871)
                     /gene="poxB"
                     /locus_tag="OO464_001775"
     CDS             complement(30123..31871)
                     /gene="poxB"
                     /locus_tag="OO464_001775"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6226948.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGT
                     PTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLM
                     GDFLSVVQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITG
                     IRVEKASEVDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIIS
                     GRGDEVIELAKTNWLR"
     gene            complement(32004..32972)
                     /gene="hcr"
                     /locus_tag="OO464_001776"
     CDS             complement(32004..32972)
                     /gene="hcr"
                     /locus_tag="OO464_001776"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6226949.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32984..34636)
                     /gene="hcp"
                     /locus_tag="OO464_001777"
                     /gene_synonym="priS"
     CDS             complement(32984..34636)
                     /gene="hcp"
                     /locus_tag="OO464_001777"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6226950.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34780..35679)
                     /gene="lysO"
                     /locus_tag="OO464_001778"
     CDS             complement(34780..35679)
                     /gene="lysO"
                     /locus_tag="OO464_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6226951.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8918 a         8848 c         9198 g         8825 t
ORIGIN      
        1 tgaccatacc gcgtgggcag ctgaaaaacc tgaaggcgag ctacacgtta acggaaccgc
       61 agcttaccgc accgctgaaa aaaggtcagg ttgtcgggac cattgatttc cagcttaacg
      121 gtaaatccat tgagcagcgt ccgctgatcg tgatggaaaa tgtggaagag ggcggattct
      181 ttggtaggat gtgggatttc gtgatgatga aattccatca gtggttcggc agctggttct
      241 cttaatcttc tgataaccgg atggcgcgaa acgtcatccg gttatacgtc attaatacat
      301 caacttaata cggtgcgtct gcgcgtactt cacatactca tcttccgggc aacaatcact
      361 caccacaata tcaaagcgtt tcaggtcacc catgcgcgcc ggacgcacct tgccaaattt
      421 actgtggtcg acaaccagca catgcttttg cgccatcgac atggcccagt gttttaccgg
      481 caactcttca agattaaaac aggtagcgcc tttactgaca tgcacgcccg ccgcggaata
      541 aaaagcgata tccgggcaaa aattattcaa cgtttgctga aaatcgatgg gtttgaaaat
      601 ggcgttgctg gcgtgaaatt caccaccgca cagaaatgcg cggcaatggg gtttctcttt
      661 cagcgccaga aaggtattta gcgaataaca aacggcggta aaagggattt cattatcaat
      721 cgcttcaata atccacggcg tggtggtgcc acagtcaaaa aagagggtct gatcgggttc
      781 taccagtgtc gcagccagtt ttgccgcccg gcgtttttct tccaccaggc gggatttttg
      841 atcgcttaac aggtaatggc tggcactgcg cggttccaga acaatatagc cgccgagcaa
      901 aacgacgggc gcactgtggt tgttcagatc gcgacgaatc gtcatctccg aaaccccaag
      961 cagggcggcg gcgtctttaa gatgtaactt atcgctgcgt tttaattctt gcagcagctg
     1021 cccgatacgc tcttcgcgac gtgtttccat aatccctctg aatagttatt gaagcgagcc
     1081 gctcaatact acacttttta gcagagatca gtcacgcacc cagcctttgc ggatcggtaa
     1141 tgcaaaacag acgcgatacc acgattgcag acgttgatag agtttatgcc ccatcgcttg
     1201 ccagataatc tgggcactaa ggcagccaat catacctgcc agcaatccac cgagcatatc
     1261 cagcggccag tggacgccaa gataaacgcg cgaccaggca atgacgacgg ccagcaccat
     1321 taaaagtgag ccggaccaca ggcgatgcca gcataaaaat gccagtgcaa aggtgaaaat
     1381 caccgtaccg tgatcgctcg ggaatgagtc atccgccgca tgatgcagga agttatagcc
     1441 gatattttcg acaaagggtc ggtcgtgcgg aaaaagatgt cccatcgtcc aggacacaaa
     1501 caggctgacc gccagcgcga tagcgatttt tatcaccagt tgccgttgtg ctgtaagccc
     1561 ccacaaccaa agtaccgcgg ccagcaacgg caccacggta atcaaatctt tagcaataaa
     1621 aatcgccaac gagatcatcc acggagccga gtctggcgtc gcgttaataa gagagaatag
     1681 agagagattc aaattttcca gcataacttc ccgacgcaaa gtgattaaaa gggagccaat
     1741 acaggcaagt cgttgagaat aaagtgcagg ttaaactggg taaagcggca tcgtcttatt
     1801 tccctcaagc ggcctgttta cggtgggtga ttgtaacggg cataggttaa ataaaactta
     1861 aagaaagcgt agctatactc gcaataatgt aagaatgtgc ttaaccgtgg tttcagctac
     1921 aaaattcgct ttctcgttag ctgcgctttt attaaactct gcgcgattat tattggcgaa
     1981 gaaattgcat gcaaaataaa ttagcttccg gtgccaggct tggacgtcag gcgttacttt
     2041 tccctctctg tctggtgctt tacgaatttt caacctatat cggcaacgat atgatccaac
     2101 ccggtatgtt ggccgtggtg gaacaatatc aggcgggcat tgattgggtt cctacttcga
     2161 tgaccgcgta tctggcgggc gggatgtttt tacaatggct cctggggccg ctgtcggatc
     2221 gtattggtcg ccgtccggtg atgctggcgg gtgtggtgtg gtttatcgtc acctgtctgg
     2281 caatattact ggcgcaaaac attgaacaat tcaccctgtt gcgcttcttg cagggcataa
     2341 gcttctgttt cattggcgct gtgggatacg ccgcaattca ggaatccttc gaagaggcgg
     2401 tttgtatcaa gatcaccgcg ctgatggcga acgtggcgct gattgctccg ctacttggtc
     2461 cgctggtggg cgcggcgtgg atccatgtgc tgccttggga ggggatgttt gttttgtttg
     2521 ccgcattggc agcgatctcc tttttcggtc tgcaacgagc tatgcctgaa accgccatgc
     2581 gtataggcga gaaactgtca ctgaaagaac tcggtcgtga ctataagctg gtgctgaaga
     2641 acggccgctt tgtggcgggg gcgctgggat tcgttagcct gccattgctg gcgtggatcg
     2701 cccagtcgcc gattatcatc attaccgggg agcagttgag cagctatgaa tatggcttgc
     2761 tgcaagtgcc tattttcggg gcgttaattg cgggtaactt gctgttagcg cgtctgacct
     2821 cgcgccgcac cgtacgttcg ctgattatta tgggcggctg gccgattatg attggtctgt
     2881 tggtcgctgc tgcggcaacg gttatctcat cgcacgcgta tttatggatg accgccgggt
     2941 taagtattta tgctttcggt attggtctgg cgaatgcggg actggtgcga ttaaccctgt
     3001 ttgccagcga tatgagtaaa ggtacggttt ctgcggcgat gggaatgctg caaatgctga
     3061 tctttaccgt cggtattgaa atcagcaaac atgcctggct gaacgggggc aacggactgt
     3121 ttaatctctt caaccttgtc aacggaattt tgtggctgtc gctgatggtt atctttttaa
     3181 aagataaaca gatgggaaat tctcacgaag ggtaaaaaaa tgcctgattg atttgtgcga
     3241 tcaggcattc tcgaattaat ggtgatggtc gtcaatctgg tgttcgataa ccatcccttc
     3301 acctacgctg gcaagatggc gaacataagg atgcgggcgg taagccggag ctggcgcagg
     3361 agccacatag acggtttgcg gcacagtcgc gacgctgacc gcttgtggaa cgctgactga
     3421 atcaggaacc acaaccactt tgtatccact cggcacatcg acggtgacac tttgcgccat
     3481 cgccacacca gtaaagccca ttaaaagcgc gcccaacagt agacaatttt tcataatcat
     3541 ttcgcctgaa ataatgcagc gggttaattt ccgcgaatta tgcagccttc attcaggcat
     3601 tgtgttgccg ggcgtgctgc cgtttttatt ggtcaaatgg cgcttcgtgt ttaagaactt
     3661 tatcgatcac atccagtacg ccttcacggt tattgtagcc tgcccggtat tttgccgctg
     3721 cgacgactgc gctgccggca ttttccattg caaaactaaa gcctgcctga cgcagcatct
     3781 caatatcgtt accgccatcg ccaaagacca ccacttcgct gtcgtctatt ccccataatt
     3841 tctgcagttg gcgaaggcca ttggctttat gtacgccggg gataatcaga tcgatgctgc
     3901 cgttgccggt gtggactggc accataatat cgccgatggc ctcatgtaat gctttttgta
     3961 cttgtggaat cagttcatcg gaaagattca ggccaaactt aaagaaaata tcttctaagt
     4021 tgtcaaagtt atcgacgtat tccagacggt gataatacat ttccgccacc gttttcatgg
     4081 catcgtcata ttttttgagt gtataggcgc tattttttcc gcaggcaata atttccactt
     4141 ccgggcgcgt cagcaaatgt tccacgacag tagcaaacgc atccttcgac agctcgccat
     4201 taaaaacatc tttgccttca ctcattaccc agccgccgtt ttcagccaca aaggcaattt
     4261 cattcgcaat ttcagggaag aaagagatca actgataata ttgattcccg ctggcgacca
     4321 caaagcgaat gccttgtgct ttcatttgct gatactgagc cataaaccgc tcacggttat
     4381 aggttttttg atcgcttaag aaagtaccat ccatgtctac cgcaattaat ttaatgctca
     4441 tcaactattc tccatcgccg tctgcgtttt ggtatcgggt ttggcgacgg ctttcgcgac
     4501 aatagccgcg agaataacca gcgccagtac aaccagcatt gcactacgta atccataatg
     4561 ttcgccgaga tagcccagca gcggcggccc gacgaggaaa gccagataac cggtcgttgc
     4621 taccacactg acgcgggttg gtgcatcggg gccggtatcg ctggcggcag aaatggtcag
     4681 cgggaagccc agcgaggcac ccagtcccca aagtacaaca gacactcctg cgacccaggc
     4741 gctatcgaca aaaataatca gcccaatacc caacgccccc attagtgcac tggcccgaac
     4801 cacggcaacg cgactgtaac tgtcgatgaa ccaaccgccg gtgaagcgtc caacggtcat
     4861 ccccagggta aaaccggcat aaatcagtga gccggaagtg gggctaaaac cgtgaccatc
     4921 aaccattaat aagggtaacc agtcgttggc agaaccttcg gcaaaggcca tcgccagcac
     4981 tacaacacca atcaacagca actggatatc gcgataaaaa ggaatgcctt tttcgccatg
     5041 ctgggtgcca tcggcagcat ttttgcccgt accgtcaggg attgcctgaa tggcgatata
     5101 aataggcgcg atacctacca gcgccgccaa taaaatgtgc accgttgccg gaacgccaaa
     5161 ggccgtcagt gccatcccga caccagcgcc tgccagcgtg cccaggctat aaaaaccgtg
     5221 catcatcggc aaaaccgttt tattcatttc tcgctcaacg gcggcacctt caacgtttat
     5281 cgccacttcc gcagaaccaa aacttgcccc aaagacgccg agaccaacgg caaagagcag
     5341 gggcgatgtc agccagagtg ccagacttaa tatcatcatc ccgatcaatg cgcaggacat
     5401 cgtgaccagg atgacattac gtgtcccaaa gcgtttcact aaccacgccg agcagagaat
     5461 accgctcatc gaaccgatcg acagaccaaa gagaacaccg cccatttcag cgatcgagac
     5521 agagagaata tcgcggatag caggcgtacg ggttgcccag gacgccatta acaggcctgg
     5581 caaaaagaag aacataaaca gcgcccaggt tcggcgtttc aatgcattac gtgaagaatt
     5641 tacggtcata gatcacgtca aaataagaag agggaagaca acattagcaa ggttgtgtac
     5701 atttgtacac aattgcagac agaggaaatg acatgcgtcg cgctaacgat ccgcaacggc
     5761 gagaaaaaat tatccaggcc acgctggagg cggtgaaact ttacggaata catgctgtta
     5821 cgcaccgcaa aattgctacc cttgccgggg taccgttggg atcgatgacc tactattttt
     5881 caggaattga tgagttgttg ctggaggcgt tcagcagttt tactgagatt atgtcccggc
     5941 aatatcaggc attttttagc gatgttagtg atgctcaagg cgcatgccag gctatcaccg
     6001 atatgatcta cagctcacag gttgcaacgc cggataatat ggagctgatg taccagctct
     6061 acgcgctggc tagccgaaaa ccgctattaa aaacggtaat gcaaaactgg atgcagcgca
     6121 gtcagcaaac gctcgaacaa tggtttgaac ccggaaccgc ccgcgcgctt gatgcgttta
     6181 ttgaggggat gacgctgcat tttgtcaccg accgtaagcc gctatcgcgc gaggagattt
     6241 tgaggatggt tgagagggtt gcagggtagt agataaattt tagataacaa aaaacccatc
     6301 aaccttgaac cgaaatggcg gggttgatgg gctccacaaa atggggacat caaagaaaag
     6361 cagtggcact aattaagact gatgccctgc ggaaaagttc tgcggttgtg caaaaaaatt
     6421 tcattttcag ggcaacttca gttttatcct aatcctggcc ataccatgac gatgattgtc
     6481 cctgccagcg tcagcaggac gttggcgatt gcgtaggtgc ccgcatagcc cagcgcaggg
     6541 atgttactgc gagctgtatc actgatgatc tccattgccg gcgcgcaggt gcgtgcgccc
     6601 atcattgcgc cgaacaacag tgcgcggttc attcgcaata cataagcacc gaacaagaaa
     6661 cagataacca cgggcacaag actgacaatt aatccggcaa tcaacatctg accgccaatc
     6721 gcgcccaggc cgttattaat accgctaccg gcgctcagac caacgcctgc cataaacacc
     6781 atcaagccga actctttcac catgcttaat gcaccttgcg gaatgtaacc gaaggtcggg
     6841 tggttagcac gcataaagcc cagcataatt ccggcgaata acaacccggc agcgttcccc
     6901 atgccgaaac tgaatgtgct gaactggaag gtgatcatcc cgatcatcag cccaataaca
     6961 aagaaggcgc aaaatgccag caggtcagtg acctggctgt gaatcgagat aaagccgatg
     7021 cgatcggcga tggtttttac gcgacgggca tcaccgctga cttgtaaaac gtcacctttg
     7081 ttaagcacga cgttgtcatc tatcggcatc tcaatctggc tacgaatgac gcggttaagg
     7141 aagcaaccgt gatcggtcaa cttcagttgt gcgagacgtt tacctacagc gttatggttt
     7201 ttaacgacca cttcttcagt gacgatacgc atgtcgagaa ggtcacgatc gaaaacttct
     7261 ttaccgttac ggaagctggg atcgagtcgg gcatgggcgt cgggatagcc taccaacgct
     7321 atttcatcgc ccatttgtag cacggcatca ccgtctggat ttgccagaat cccgttacgt
     7381 cgaatacgtt caatgtagca gccggtttgt cgataaatac ccagttcacg cagatttttg
     7441 ccgtcggtcc aggccaccag ctccgggccg acgcgatagg cgcggatcac cggtaaataa
     7501 accttacggt tggcatcagt gtccaggcca cgttcgcggg cgatttgctg ggcgctggtc
     7561 tgtaagtcct gatgctgcaa tttcggcaag taacgcgcac caacaatcaa actcaccaga
     7621 ccgattaaat aggttaaggc atacccgagg ctcagattat ccagtgccag tgagagctgc
     7681 ctgctttcca tgccggaatg atgcagtgta tcgccagcac cgaccagaac cggtgtcgac
     7741 gtcatagagc ctgctaacat accggccgtc aggccaatat cccagccaaa cagcttacct
     7801 aaccctaagg cgatcaccag cgcactgcca accatcacca gtgctaacat taggtaattt
     7861 ttcccatcgc gaaaaaaaat ggaaaaaaag ttcggtccgg cttcgacccc gacgcagaaa
     7921 ataaacagca taaagccaag attaagcgca tcggtgttaa tgctgaaatg ttgttggcct
     7981 aataacagcg atacgactaa aacgccaatg gaattaccca gttggatcga accaagtcgt
     8041 aactttccga gacatagccc aagcgcgagg accacaaata ataacagaat gtaattccca
     8101 tttaacaatt cggcgacgtt tatattcacg gaggctaact tcttgtttac tagtaagctg
     8161 ttgaaagaaa tgctaattta cgataatgtg ttttaccaga attcagggcg cagattcatt
     8221 cagcgcacct aaacgatagt aaagtaacaa tatattttac tagtgtaatc acattaggta
     8281 tcaacggcta tatgaattgc gttggcctat attagcatgg aatgcgaagc ggctttatct
     8341 tactgaacgc cacactggcg aaaaatgtgt tcgatagacg cagtgtcagg aggaacgagt
     8401 gaaacataaa caacgttggg cgggggcaat ctgctgtttt gtcctcttca ttgtggtgtg
     8461 cctttttctg gcgacgcaca tgaaaggcgc ttttcgggct gccgggcatc ctgaaatcgg
     8521 cttgctgttt ttcattcttc ctggagcagt tgccagtttc ttttcacagc gtagagaagt
     8581 cctgaaacct ctatttggcg caatgctggc ggcaccctgt tcaatgctca ttatgcggct
     8641 gtttttttca ccgacgcgct cattctggca agagctggca tggttactaa gcgcggtgtt
     8701 ctggtgtgcg ctgggggcac tgtgtttctt atttatcagt agtttgttta aaccacagca
     8761 cagaaaaaat cagtaaagcc ctcaacgcga gggcttgtca gacgatcagg cgtccagatt
     8821 ttctttcacc catgcagcaa aatcggtata gccgccgata tgttgctgat cgacaaaaat
     8881 ctgcggcacg gtttctacgg gtttacctgc cttttgttgt agatcttctt tagtgatccc
     8941 ttccgcacga atatctacat actgatactg aaaatcatcg cgttcattgc tcaatttctc
     9001 agccagatct tttgcacgca cacagtaagg gcaacccgaa cgaccaaaaa taacggtttg
     9061 cattatttct ctcctcatag atttatgcct gtaatgatca cgctaaaatg tattcgctga
     9121 aagtaggttt aacctgttgc attaattgct aaaagctata actgttaaac acaatacagt
     9181 gaaaagtttt agactgaagg ctcactttgc agagggaagc gtatgcgcgc gatcggtaaa
     9241 ttgcctaaag gcgtgttgat actggaattt atcggaatga tgctactggc ggtggcgctg
     9301 ctgtcggtaa gcgactccct gtcgctgcct gagccatttt ctcggccaga agtgcagatt
     9361 ctgatgattt ttctcggtgt tttgctcatg cttcccgctg cggtggtggt tattcttcag
     9421 gtggcaaaac gtcttgcccc acagctgatg aactgtccac cgcaatattc acgttcagaa
     9481 agagaaaaag ataatgacgc caaccattga acttatttgt ggccatcgcg ccattcgcca
     9541 tttcactgat gaacccattt ccgaagcgca gtgtgaggcg attattaaca gcgcccgtgc
     9601 gacgtccagt tccagttttt tgcagtgtag tagcattatt cgcattaccg acaaagcatt
     9661 acgtgaagaa ctggtgacgc tgaccggcgg gcaaaaacac gtagcgcaag cggcggagtt
     9721 ctgggtgttc tgtgccgact ttaaccgcca tttacagatc tgtccggatg ctcagctcgg
     9781 cctggcggaa caactgttgc tcggtgtcgt tgatacggca atgatggcgc agaatgcatt
     9841 aaccgcagcg gaatcgctgg gattgggcgg ggtatatatc ggcggcctgc gcaataatat
     9901 tgaagcggtg acgaaactgc tgaaattacc gcagcatgtt ctgccgctgt ttgggctgtg
     9961 ccttggctgg cctgcggata atccggatct caagccgcgt ttaccggcct ccattttggt
    10021 gcatgaaaac agctatcaac cgctggataa agacgcactg gcgcagtatg acgagcaact
    10081 ggcggaatat tacctcaccc gtggcagcaa taatcgctgg gatacctgga gcgatcatat
    10141 ccgccgaaca atcattaaag aaagccgccc atttattctg gattatttgc acaaacaggg
    10201 ttgggcgacg cgctaaaacc gccacgtcga tgtatgatac gcgggctttt gaccaggtct
    10261 gacagagagg tgcagggtga aaattgccat attgtcccgg gatggaacgc tctattcgtg
    10321 taagcggctg cgtgaagccg ctatacagcg cggtcacctg gttgaaattc ttgatccgct
    10381 ttcttgctac atgaacataa atcctgcggc gtcttctatt tactacaaag gccgcaagtt
    10441 accccatttt gacgcagtga tcccgcgtat tggcaccgcc attacctttt atgggacggc
    10501 ggcactgcgc cagttcgaga tgctggggag ctatccgctc aatgagtcgg tcgccattgc
    10561 ccgggcgcgt gacaaattgc gttccatgca actgctggcg cgtcagggca tcgacctgcc
    10621 tgtcacgggc attgcgcatt cgccggatga taccagcgat ttaatcgaca tggtcggtgg
    10681 tgcgccgctg gtggtcaagt tggttgaagg cacgcaggga attggcgtcg tgctggcgga
    10741 gactcgtcag gcggcggaaa gcgtgattga cgctttccgc ggtctgaacg cgcatattct
    10801 ggtgcaggaa tatatcaaag aggcgcaagg gtgcgatatc cgctgtctgg ttgttggtga
    10861 tgaagtggtc gctgcgattg aacggcgggc gaaagagggc tattttcgtt ccaatttgca
    10921 tcgtggcggc gcggcaagcg tcgccagtat cacaccacag gagcgtgaaa tcgcgataaa
    10981 agccgcgcga acgatggcgc tggacgttgc tggtgtggat attctgcgtg ctaatcgcgg
    11041 gccgttggtg atggaagtga atgcgtcgcc ggggctggaa ggaatagaaa aaaccaccgg
    11101 tatcgatatc gcgggtaaaa tgatccgctg gatcgaacgc cacgctacga cagaatattg
    11161 cctgaaaacg ggtggttagt cgcaatcaca ttactgatca tggttttgcc tgcgcttttt
    11221 gcgtaagctg tgccggtctt tttatcgaaa gaggttgtac aaaattatga catcgctggt
    11281 cgttcctggt ctggatacgc tgcgtcaatg gctcgatgac ctggggatga gtttttttga
    11341 atgtgataac tgtcaggctc tgcatctgcc ccatatgcag aatttcgacg gtgtctttga
    11401 tgccaaaatc gatctgatcg ataacacgat cctgttttct gccatggcgg aagtccgacc
    11461 ttcagccgta ttgccgctgg cggcggattt atctgccatc aatgccagtt cgctgaccgt
    11521 gaaagcattt cttgatatgc aggatgataa tctgccaaag ctggtggttt gccagtcttt
    11581 atccgttatg cagggcgtaa cctatgagca gtttgcatgg ttcgtgcgtc agagcgaaga
    11641 gcagatttcg atggtcattc ttgaagctaa tgcccatcaa ctgctgttac cgactgacga
    11701 tgaagggcaa aacaacgtta ccgaaaacta ttttctccac tgataattcc tttcgagcac
    11761 gcagtcgctg ccgcagtgac tgcgcgctgc aaaattatct gctgttttta accttttctt
    11821 aaagattatt tcacttctct tgtgtcgatt tggctttatc acatagagca aatacgcata
    11881 aaaatttgtt aaataccgtt ttttaatccg agctatagtc tcaaaccctg gctaaagtta
    11941 ttcttgcgat gcttctatat agcgagcagt gctggccggg agagagttct cttttcttac
    12001 accgcgccga taaaaaaata tgcacgttta ttgcatatct ttcagtgtga caacttttgt
    12061 tcgtttgtta acgaactttc agaaggaaag agatgtgacc gccttaaata aaaaatggct
    12121 atcgggtctg gttgcgggtg ctctgatggc cgtctctgtc ggcacgctcg cggctgaaca
    12181 aaaaacactc cacatttata actggtctga ttatatcgcc cctgacacgg tggccaattt
    12241 tgaaaaagaa accggtatta aagtcgtcta cgatgttttc gactctaacg aagtactgga
    12301 aggcaaatta atggcgggta gcaccggctt tgacttggtg gttccatctg ccagctttct
    12361 agaacgccag ttgactgcgg gagttttcca gccgctggac aaaagcaaat tgccggagtg
    12421 gaagaatctc gacccggagc tgctgaagct ggtcgccaaa cacgatcccg acaataaatt
    12481 tgctatgccc tatatgtggg cgacgaccgg gattggctat aacgttgata aagttaaagc
    12541 tgtgctgggc gaaaacgcgc ccgtcgatag ctgggacttg atcctcaaac ctgaaaatct
    12601 ggaaaaactg aaaagctgcg gtgtctcttt cctggatgcg ccagaagaag tttttgctac
    12661 cgtgttgaat tatctcggca aagatcccaa cagcactaaa gcggatgatt acaccggacc
    12721 ggcaaccgat ctgctgttaa agctgcgccc gaacattcgt tatttccatt catctcaata
    12781 cattaacgac ctggcaaacg gcgatatttg cgtcgctatc ggctgggcag gtgatgtctg
    12841 gcaggcgtca aaccgcgcga aggaagcgaa gaatggagtg aatgtctcgt tctcgattcc
    12901 aaaagaaggg gcgatggcgt tctttgatgt attcgccatg ccagcggatg ccaaaaacaa
    12961 agacgaagcc tatcagttcc tgaattacct gctgcgcccg gatgtagtag cgcatatttc
    13021 cgaccatgtg ttctatgtca acgccaataa agcagccacg ccgctggtga gtgcggaagt
    13081 ccatgagaac ccgggtattt atccgcctgc ggatgttcgt gcgaagctgt tcactctgaa
    13141 agtgcaggac ccgaaaatcg accgtgtgcg cacccgcgcg tggaccaaag tgaagagcgg
    13201 aaaataatcc gcagccttag atgccgggcg ggcgcaccac acccgccggc aattcgcacc
    13261 atcatggtgc gcttgcacac attcaatgcc ggagagcagc cgtgaatgac gctatccctc
    13321 gcccgcaggc gaaaacccgt aaggcgctga cgccgctatt agaaatccgc aacctcacca
    13381 aatcctacga tggtcaacat gcggtggatg atgtcagcct gaccatctac aaaggtgaaa
    13441 tcttcgcgct gctgggcgca tccggctgtg gcaagtccac gctgctgcgt atgctggcag
    13501 gtttcgaaca accttctgcc ggacagataa tgcttgatgg cgtcgatttg tcacaggttc
    13561 cgccttacct gcgccccatc aatatgatgt ttcagtctta cgcgctgttt ccgcatatga
    13621 ccgtggaaca gaacatcgct tttggcctga aacaggacaa actaccgaaa gcggaaattt
    13681 ccagtcgggt caatgagatg ctcggcctgg tgcacatgca ggagttcgcc aaacgtaaac
    13741 cgcatcagct ttctggtggt cagcgacagc gtgtggccct ggcccgaagc cttgccaagc
    13801 gcccgaaact attactgctc gacgagccga tgggcgcgct ggataaaaag ctgcgtgacc
    13861 ggatgcagct tgaagtggtg gatattctgg agcgcgtcgg tgtgacttgt gtgatggtca
    13921 cccacgatca ggaagaggcg atgaccatgg cggggcgcat cgccattatg aatcgtggga
    13981 aatttgtcca gattggcgaa ccggaagaga tctacgagca tccgactacc cgctatagcg
    14041 ccgaatttat tggctcggta aacgtctttg aaggcgtact caaagagcgt caggaagatg
    14101 gcttggtgct tgattcgccg gggctggtgc atccactgaa agtcgacgcg gatgcctcgg
    14161 tggtcgataa cgtgccggta catgtggcgc tgcgcccgga aaaaatcatg ctttgcgaag
    14221 agccgcccgc caatggttgt aacttcgcgg tgggggaggt gatacacatt gcctatctcg
    14281 gcgatctttc ggtgtatcac gttcgtctga aaagtgggca gatgattagt gcccagctac
    14341 aaaacgccca tcgtcatcgt aaagggttac cgacctgggg cgacgaagtg cgtttgtgct
    14401 gggaagtgga cagctgtgtg gtgctgacgg tttaaggagc aaagatgaat acacttgaac
    14461 ctgctgccca gtcgaaaccg ccgggcggat ttaagctgtg gttgtcgcag ctgcaaatga
    14521 agcatgggcg caaactggtc attgcgttgc catatatctg gttgatcttg ctgtttctgc
    14581 tgccatttct gattgtcttt aaaataagcc tggcggagat ggcgcgcgct attccacctt
    14641 ataccgaact gatggagtgg gctgacgggc aactgtccat cactcttaat ctcggtaatt
    14701 ttctgcaact gaccgacgat ccgctctatt tcgatgctta tctccagtcg ctacaggtgg
    14761 ctgcgatttc gacattttgc tgtttactga tcggctatcc gctggcgtgg gcggtggcgc
    14821 acagtaagcc ttcgacccgt aatattttat tactactggt gatcctgccg tcgtggacct
    14881 cgtttctgat ccgcgtttat gcttggatgg gaatattaaa aaacaacggt gtgctgaata
    14941 attttctgct gtggctgggg gttatcgatc aaccgctgac cattcttcat accaatctgg
    15001 cagtttatat tggcattgtt tacgcctacg tgccgtttat ggtgctgccg atttataccg
    15061 cgttgattcg tattgattat tcgctggtgg aagcagcgct ggatctcggt gcacgaccgc
    15121 tgaaaacgtt ctttactgtg atcgtgccgc tgactaaagg tgggattatt gccggatcga
    15181 tgctggtgtt tatcccggct gtgggcgagt ttgtgatccc ggaactgctc ggtggcccgg
    15241 acagcatcat gatcgggcgc gtgctatggc aagagttttt caataaccgc gactggccgg
    15301 tggcctcggc ggtagcgatc atcatgttgc tgctgctaat tgtgccgata atgtggttcc
    15361 acaaacacca gcaaaaaagc gtgggagaac acggatgaat aatttaccgg tagttcgttc
    15421 gccctggcgg attgtgattt tgctgctggg ctttaccttt ctctacgcgc caatgctgat
    15481 gctggttatc tattcgttta acagctcgaa gctggtgacg gtgtggggcg gctggtcaac
    15541 gcgctggtat ggtgagttat tgcgcgatga cgcgatgatg agtgcggttg gttcaagcct
    15601 gacaattgct gcctgtgcgg caacggcggc ggcgatcctt cgggactatt gcggcggtgg
    15661 tgctggtgcg ctttggcagg tttcgcggat caaatggctt tgcctttatg atcaccgcgc
    15721 cgctagtaat gccagatgtc atcacgggct tgtcgctgtt gttattattc gtcgcgcttg
    15781 ctcatgccat tggctggcct gcggaccgcg gtatgcttac catctggctg gcgcatgtca
    15841 ctttctgtac ggcttatgtg gcggtcgtta tttcgtcgcg tctgcgggaa ctggatcgct
    15901 cgatagaaga agccgcgatg gatctcggtg cgacgccgct gaaagtgttt ttcgtcatta
    15961 cgctaccgat gatcatgccc gcgatcattt ctggctggtt actggctttt actttgtcgc
    16021 ttgatgatct ggtgatcgcc agctttgttt ccgggccggg agccaccacg ttaccgatgc
    16081 tggtcttttc cagcgtgcgg atgggggtga atccggaaat caacgccctg gcaacattga
    16141 tcctcggcgc ggtcggaatt gtcggattta tcgcctggta tctgatggcg cgcgcagaaa
    16201 aacagcggat acgcgacatc caacgtgcaa gacgtggctg aagacactaa aatttgccaa
    16261 cctggctaca taatgccgcg catgtcgcgg cattgttttc atggaagacg aaacgttggg
    16321 attttttaag aaaacatctt catctcatgc tcgcctgaat gtgcctgcgc tggtgcaggt
    16381 ggcggcgctc gccattatta tgatccgtgg cctcgacgtg ctgatgattt tcaatacgct
    16441 gggcgtgcgc ggtattggcg agttcattca tcgcagcgta caaacctgga gtttaacgct
    16501 ggtcttttta agcagtctgg tgctggtttt cattgagatc tggtgtgcgt tttcactggt
    16561 gaaagggcgt cgctgggcgc gctggctata tctgctgaca caaatcaccg ccgcaagtta
    16621 cttgtgggcg gcttcgctgg ggtacggtta tccggagctg ttcagcattc ccggtgaatc
    16681 aaaacgtgaa atcttccata gcctgatgct gcagaagctg ccggatatgc tcatcctgat
    16741 gctgctgttc gttccctcga ccagtcggcg gttcttccag ttgcaataat gtgtataatc
    16801 gtcgcccctg atgatgtgaa ggtcaatgta tgcagtgcgc actttacgac gcgggtcgct
    16861 gtcgttcctg tcagtggata acgcagccga ttccagagca actctccgct aaaaccgccg
    16921 atcttaaaaa tctgctcgcc gattttccgg ttgaggaatg gtgcgcgccg gtgtcaggcc
    16981 cggaacaagg gtttcgtaat aaagccaaaa tggtggtgag tggtagcgtt gaaaaaacac
    17041 tgctcggtat gctgcatcgc gatggcacgc cggaagacct ttgtgactgc ccgctttatc
    17101 cagcctcatt tgcgcccgtt tttgcggcgc taaaaccgtt tatcgcccga gcggggttaa
    17161 cgccctacaa cgtggcgcgt aagcgtggcg aactgaaata cattctgctg actgaaagcc
    17221 agagtgatgg cggcatgatg ctgcgctttg tactgcgttc tgataccaag ctggcgcaac
    17281 tgcgtaaggc gctgccgtgg ttacacgaac aactaccgca gctgaaagtt attaccgtca
    17341 atattcagcc ggtacatatg gcgattatgg aaggggagac ggagatctac ctgaccgaac
    17401 aacaggcact ggcggagcgt tttaatgacg taccgctgtg gatccgtccg caaagtttct
    17461 tccagactaa tccggcggtc gccagccagt tgtacgccac cgcgcgcgac tgggtacgac
    17521 agctgccggt taaacatatg tgggatctgt tctgcggtgt ggggggcttt ggtttacact
    17581 gcgcgacgcc tgacatgcag ttaaccggga tcgaaattgc gccagaggcc attgcctgtg
    17641 caaagcagtc agccgctgaa ctgggcttaa ctcgtttaca atttcaggcg ctggactcca
    17701 ctcagtttgc cactgctcag ggggaggtgc cggagctggt gctggttaac ccgccgcgcc
    17761 gcggcattgg taaaccgctg tgtgattatc tttcaacgat ggcaccgcgt tttattatct
    17821 actccagctg taacgcccaa accatggcga aagatatccg cgaactgcca ggttaccgca
    17881 tcgaacgggt acagcttttt gatatgttcc cgcacaccgc gcactatgaa gtgttgacgc
    17941 tgctggtgaa gcaataaaaa agccacaggt gcggcttcag attgctgatc aggtgcgctt
    18001 tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaatcatg ctaaatcaat
    18061 acattgcaga gttgatgtgg gccggataag cgcagcgcat caggcaatct tacttttgtc
    18121 atcagtctca attactgcgg aaaccactgg tcattgattt tctgatatgt accgtcagct
    18181 ttaattgctg ccagcgcgtt attcagtttt tccagcagag ctttgttatc cggacgtaca
    18241 gcgataccca ggccggtgcc gaagtattgc ggatcggtca ctttttcagt cgcaacgccc
    18301 agttgcggat tggttttcag ccattcgttt accaccgcgg tgtcaccaaa taccccatca
    18361 atacgaccat ttttcagatc gataaaggca ttctgataac tgtcatagga gacagttttc
    18421 acttccgggt gctgatcctg aatatatttc tggtgcgtcg taccgttttc catcccaatg
    18481 cgtttgcctt tcaggtcggc aaacgttttg taggtatctt ttttggaaat cacgacggct
    18541 gagttttcat agtagggtgt ggtaaacgaa acctgcttgc tacgctccgg ggtgatatcc
    18601 ataccggaga ttacggcgtc atattttctg aatttcagtg acgggatcag gctgtcgaac
    18661 gcgtgattag taaaagtaca ttctgcctgc atttgtttgc acaaggcttt tgccagatcg
    18721 atatcaaagc cgacaatctc attattagca cctatagatt caaagggtgg ataggtggct
    18781 gaaacgccaa aattgatttt ctctgcggca gaagcaccga aagtaaagga agcaagtaaa
    18841 gcggcaagaa ctaacttttt catgatggaa ctcccgtctg tcaatcttat gatttttggc
    18901 cgtgtctgcg gcatgggata acaatgccat caagtgaatt tatatgcaat aaacatgatt
    18961 aaataattta aatgaaataa aaaaagacgg ataacttagt gagttgtccg tcttcattat
    19021 aagaatttat gcactatgta ggccggataa ggcgtccacg ccgcatccgg cacaggcacc
    19081 gtgctgatgt ctgatgcgac gctggcgcgt cttatcagac ctacaaaccc ccccggcgaa
    19141 tgtacgcagc tacattaatt ccgccgttcg aatgccagtg ctttgcgctc gatcagacgc
    19201 atcatcagcg tcagcaggcc gttgacgacc aagtaaataa tccctgccgc accgaacacc
    19261 attacatcgt aggtgcgtcc gtacaacaac tggctgtatc ccatcacttc catcagcgta
    19321 atggtgtaag ccagagaggt acttttgaac accagcacca cttcgttgga ataagaagag
    19381 agcgagcgtt taaaggcata cggcagcagg atcgccagcg tatctttttt gctcattccc
    19441 agggcgctac aggactgcca ctgaccttct gggatcgcac gaattgcacc gtaaaacagc
    19501 tgcgtggtat acgccgcact gttcagcgat aacgcaatca gcgcacataa ccacggttct
    19561 gacaacaaat gccacagtgc cggatactcc tgcaacgtcg gaaactggcc cggcccgtaa
    19621 taaatcagga agatctgcac cagcagcggc gtaccggtaa acagcgtgat ataaccccgc
    19681 accagccaca ccagcaccgg cgttttcagc gtcaggatga tggtaaaaat caatgccaga
    19741 atcagtgcca caatcagcga ggcaacggtt agcgtcaggc tggtgtgcag ccctttcatc
    19801 agctcgggta aatactcaaa cattagctgg gcctccgctc aaaacgtgtc gcgcgcaggt
    19861 caatgcgttt gagaatgtac tgactgagca gggtgatcac caggtaaatc gccgccgcca
    19921 caatgtacca ggtaaatggt tcctgggtac gagtagcaat gcttttggtt tgtaacatta
    19981 aatcattcac actaatcaaa ctgaccagcg cggtatcttt cagcagcacc agccactggt
    20041 taccgaggcc aggcagcgca tgacgccaca tctgcggcat caccagacgg aaaaagatag
    20101 ccgatttcga cagccccagc gcctgaccag attcccactg gcccaccggt acagctttca
    20161 acgcgccgcg cagcgtttgc gaggcatagg cggcatacag cagtgacaga gcgatgacac
    20221 cacaaaggaa cggactcacg tcgaagttct caatgtccat ctgcaccggg atctgcacga
    20281 acccaagatt gatagtgaag ccatccgaaa gcgtcagcag taactgcgag gagccaaaat
    20341 agataaacag cactaccaga atttctggca ggccacgcag aatggttacc agcgctgaac
    20401 ctgcccacgc gacaggacgc catttcgctg actcccatac cgcaaagaac atcgccagcg
    20461 ccagcccgac aatcaatgca caaacggcaa ggccgacggt catcccggcg gcgcttgcta
    20521 aaggaaaaaa ttcattcatc aggaattact tctggaacca tttgttgtag atggtttcgt
    20581 aagtgccatc tttcttcact ttttccagcg cagtgttgag tttctgctgc agttcagtgt
    20641 ttccctgacg taccgcgatg ccgaggccag taccgaaata atctttatcg gtcactttat
    20701 cgcccactgc cgccagtttt gggttatctt tcagccactc agtgaccact gcggtgtcac
    20761 caaatacgct gtcgatacgc ccgttttgca gatccagttt tgcgttctgg tagctgtcat
    20821 acggaacggt ggtgatttcc gggtgcttat ccataatgaa cttctggtgt gtcgtcccgt
    20881 tctgaacgcc gacttttttg cctttcagct gatcaacact ggtgtatttg ccttgctgac
    20941 ccacaaacag ggcagagttg tcatagtacg gggtagtaaa cagtacctgc ttttcacgct
    21001 ccggagtgat atccatgccc gccatcactg cttctacgcg acggaatttc aggcttggga
    21061 tcaggctgtc aaacgcctgg ttagagaaag tacaggttgc atcaatctct ttacacagtg
    21121 cctgcgccag gtcgacgtca aaaccaacga tctggttgtt tgcatcaatc gattcaaacg
    21181 gaggataggg ggcttcggta gcaaaacgaa tggtttcggc agctgtggcg gaaagactaa
    21241 aacctgcaat taacgcggca atcagaactt ttttcattgt tgttatcccg aatcttagtg
    21301 agagagatag tttttaaatg cttcggtttg cggctcggta aagcagctcg cgtcgccttg
    21361 ttctacgata tgaccatttt ccatatacac cactcggctg gcggttttac gcgccacttc
    21421 aacttcgtgg gtgacgatca cctgggtaat attcgtttct gccagctcac gaatgatgct
    21481 gacgatttgt gccgtaattt ccgggtccag tgcggcggtc ggttcatcga acagcagtac
    21541 ctgcggttcc atcatcaacg cacgggcaat agcaacacgc tgctgctgac caccagaaag
    21601 atgcagcggg taacgatcgc tataaggttt gagacgcaga cgttccagca gtttttctgc
    21661 acgggccagc gcctgatctt tactcaaccc cagtacacgg cagggcgctt caatcaggtt
    21721 ttgctgcacg gtcagatgcg gccacaggtt gtattgctga aacaccatgc caacgttacg
    21781 acgcaaatcg cgaatcgctt tgtcagaggg tgttttggtg aaatcgaaat ggttgcctgc
    21841 aatgttgagc gtaccggagc gcggcatctc aagcagattg agtacacgca gcagcgagct
    21901 tttacccgcg ccgctggggc caagtaacac cagcgtttcg ccctgtgggc aatccagcgt
    21961 gatatcgaac agcgcctgat gcgcgccgta gaagcaatta atgccgttta attgaatact
    22021 cattgacact cgtatactgg cagtctgata gctattgagg tcgaagatag tacctttaac
    22081 agaataatta tgcaatattt atgctttaaa agttaaaagc aaagcgcatt attcaataaa
    22141 catagcacaa aataacgggg gcggtggtcg gtgagcataa atgtcggcat tcctcacgaa
    22201 atgccggata atttacgggg tttattggtt gatcaaggcg ttagcgattc tcgatggact
    22261 gacggagcgt acccgccgtg gcatgaacgc taccgcctaa gtagcgcaca tcgtcgatga
    22321 cccaacactg gccttcctga atcattaaca cttcatcctg ccagccctgg tcaccctgtt
    22381 tgagatccac ccgcaatgga atgttacggg catcacgatt agggatagtc gatgcactgg
    22441 caacgtgggc gctgtctggc aaggtggttc gactggagaa cggatcgctg cttaacaatt
    22501 cacgatgact attatcgcgg ctggcatcgc taagttgggt tgccagtttg tcactcaaat
    22561 aggggcgcag ggcggtaatg tcattgctgc gatgttgaat gcggtagtcg tagaattgtt
    22621 gagccacact gtcaggccct ccctggacac aggaaccggt gcgggggccg ttatctttat
    22681 aagctggcgt gacggtggta caggcactca gcagcagtgc acatggaata agtagagata
    22741 gtgaacggta gcgcataatc atttccttat tagcttgctg tctgcgtcac actacgacca
    22801 aacgctatca gattatttaa tacattgttt ttaatacaat tctgaaacaa tctgaacacc
    22861 tgattgggtt tgagtccatt gctggcgacc cactcaaggt tgctcgtagc tgacttactt
    22921 aaattaccca aacaatagac agtgtaaacc atttgtgagg ttgagcgtgt tatatggctg
    22981 aaatgatcat ttaaggtaaa tatgatagtt ctgaaatggt ggtataaatc ctcctgtaga
    23041 ggttaagagc atctgcaaga agccagtttc acatttataa cgcgtgataa atgaatgcgg
    23101 ttattgtcta atcattacaa aatttttcac atgcgataag agatcctgcg attaagatga
    23161 ttgccaaaat tacgttgtat ttttgaaaat gagcgaaatt tgatagtccc agctttgact
    23221 ctcgataata ttatttatgc tacaattgtg ggtaaagaat ttaagctaat gaatttgagt
    23281 gaagaataaa ttaaattgct tcagtatttc gttaattaat ataaatacaa aaggcgttgt
    23341 gcttttataa ttataaagcg cgaggagttc ggtggattat cgtttttctt gggtgatttt
    23401 taatatgata aacaatatat ctgatcaagc atcatcattt ccaggcacac aactaaatca
    23461 aagtgataat ttcctggatt cactgaggga atttttcgcg atattaaatc ccagtcgtaa
    23521 gggtgagttg tctacatggg ataccatcta cttgcatctg atattagcaa taaatgctga
    23581 cagtgatctc ataaaaaatg atgtgttgct cgcggaaaat attccatcag cgaattacca
    23641 atttaataca ttttttagca atactttcga gattgatgtt aaaaaatatc tgggtaaatc
    23701 agaagataac gaagttgaaa taaaagcggg taatgaaaga atctctattg gaatacgtaa
    23761 tgtatcgaat ggaaaacttg aaagacaaca gtttcttttc ccactggact atgagaataa
    23821 attacaagaa caattagata agtattttac gattgaatcg cacccattat tatacaggta
    23881 taccattggt agtaagattg cgaatgtgat ttttgaaaag ttatacagtc gtattgactt
    23941 taacaaagaa caatatatat cctttataaa ggacgcattt attcattttt atgattatag
    24001 caggcgatat gctattagtg agaatatcga taaagacgct gtaacaaaca atatcgcatt
    24061 gatgagcact ttttatgaca gtgataatac gtcaggtgag gttttaaata atgattttac
    24121 agaagaagag tcatttgaaa ctgcgttaga tgtagagcat gcaatagtgc ttggatttgc
    24181 tgatgataat tttgaaacaa aacctgttca ttatcaggat ttattaacac gatttagtgc
    24241 ctttcaagat accgtgttta atctgtttcc tgaaatgcac tcttcgcatt accacgatat
    24301 ttgtagcgta agtgtggata tgacaaaggg aacgcaatgc atgattcact tgatggtgaa
    24361 tgaagaagtt ttcatgtctc tgcctgttcc agttgccacg atggtacgtg aagatgcaag
    24421 taatttagtc aatcttaaaa cactactcaa tgatggttgt ttcattaaat atagtcattt
    24481 taatgacgtg gcattaataa agcaaaacat aagtaatctc tatttatcac acactgttgt
    24541 gaatgaaagc atattgaaaa aatgttgttt tgaaaacggc agtttaggtg atgttaaaat
    24601 aacaaatagc aatgtaataa acagtgcatt caaaaatatt tcgtttagat ctgttaaaat
    24661 taataacgta aatactcatt ctttaaaatt tattaactgc aattttttca atgttgatat
    24721 gataagagtt aatctcagca aatgtctttt tcatgaatgt agtatgcatg gtgtcaagat
    24781 aaaaccctgg ttgcctgtaa aatggacaaa agaattaata agtgattatt tgtatggctg
    24841 tttactatct ctgtactcta tctgtgcaag ggatatatat aatatgaacg ccgggaataa
    24901 cgtgaaggtt gctgcggatg ctttccttga aattattttc tctcttaaaa ataaatattg
    24961 tattaaacta ttaagcgctc aggatcgggc gtttatatac gagtttgcaa gaatgatctt
    25021 cgcttatatt aatgacaaaa gcattgagat cttattactt tcttgttttg cggcggctga
    25081 ccaaaaagca atccagcggt atagacctca gtcacaagat ggggaagact ttaggagtca
    25141 tttacaatat aagttgcctc tttccgctca ttaatgttgt tggtgcaaaa taataagaca
    25201 ggtaagcggt accctataat gaaaccaata tcgggttatg caacggataa tcgtaaagac
    25261 ttaagtgggt gtttttgaca caatgagatg atttcctaaa ctgatcaaaa ccttcttccc
    25321 aacatggcat atcagtccga taatgcttta gagatcgaag gcttaacaaa caaggagatc
    25381 gatcatgcaa ttttctacaa ccccaactct ggaaggcctg accatcgttg aatattgcgg
    25441 tgtggtgacc ggcgaagcga ttttaggtgc caatattttc cgtgatttct ttgccggtat
    25501 ccgcgatatc gttggcggac gttccggtgc gtatgaaaaa gaactgcgta aagcacggga
    25561 gatcgccttt gaggaattag gctcccaggc gcgggcgctg ggggccgatg ccgtagttgg
    25621 tattgatatc gactacgaaa cggtcgggca aaacggcagt atgctgatgg ttagcgtcag
    25681 cggtacggcg gtgaaaacgc gtcgatgaga agagtcttct ggctggtcgc tgccgctctg
    25741 ttactggcag ggtgtgcagg cgaaaaaggc attgtcgaga aagagggata tcagcttgat
    25801 acccgacacc aggcgcaggc ggcgtatccg cgcattaaag tgctggtgat ccactacacc
    25861 gcagatgatt ttgatagctc gctggcgaca ctgactgata agcaggtcag ctcgcattat
    25921 ctgatccctg cggtgccacc gcgatacaac ggtaaaccgc gcatctggca actggtgccg
    25981 gaacaagaac tggcctggca tgcggggatt agcgcctggc gcggggcaac gcgccttaac
    26041 gacacctcta ttggcattga gctggaaaac cgtggctggc aaaaatcatc cggagtgaaa
    26101 tattttgccc cgtttgaaca ggcacagatt caggcgctta ttccgctggc gaaagatatt
    26161 attgcccgtt atcacatcaa gccggaaaac gtagtggcac atgcggatat cgcaccgcag
    26221 cgcaaagacg atccggggcc attatttccc tggcagcaac tggcgcagca ggggattggt
    26281 gcctggccgg atgcgcagcg ggtgaacttt taccttgccg ggcgcgcgcc gcacacaccg
    26341 gtagataccg cgtcattgct ggagcttttg gcgcgctacg gttatgacgt aaaacctgat
    26401 atgacaccgc gcgagcagcg gcgcgtgatt atggcattcc agatgcattt ccgcccgacg
    26461 ttatataacg gcgaagcgga tgcagaaact caggcgattg ccgaagcatt gctggagaaa
    26521 tacgggcagg attagcgcgg cagttttccg tggtcgcgta gccaggcggc agttttctcg
    26581 atgccttcat ccagggtgat gaccggctga taacctaact cttcctgcgc acgcgtaata
    26641 tccagcgtaa agtcaaaatt caacttggag gcgccgtagt gggtcagcgg cggctctttt
    26701 gctgacttgc ggcctaaacg ctccatgctg cgggcgatca tatccagcat cgggtaaggg
    26761 acggaacgaa tacgacagtc aatattcaac tcgtcgatca gcttctgcac gatgctgcgc
    26821 agtgtgcgat gctcgccgtt ggtgatgttg tacacacgcc cggaaggtag cttatcgcag
    26881 gcttcctggc ttgccagcca cattgcgtgc acggcatttt catagtaggt catatcgacc
    26941 agcgcactgc cgccatgcgg taacagaata ctgccgtagt ggtgcatcat atgcgccaga
    27001 cggggaataa agactttatc gtgcggtccg aacagacttt gtgggcgcag aatagtaaag
    27061 cgcgtttgtg gattcgcctg cgaaagcata ttgatcactt cttcgctggc tgctttgctg
    27121 cgggcaaact cgttggcgaa gcggtgaggg cgaaaatctt ctttaatatt gcgatggtgg
    27181 tgataatcga agtacaggga gggggaagag atatgaataa agttacgcac accccaggcg
    27241 acagcccatt cacccaggcg gcgagtggcg cgaacgttag ccagatcgaa agcctgttgt
    27301 gtcccccagg gtgaggtaaa gctggagcag tgccacagcg tatcaatgcc cgcgagcatc
    27361 actttagctt gtgatgaaac cagctcggtc agatccgtcg gaacaaactc tgcgcccatt
    27421 ttttccagca atttacccat tgcttcgttg cgaccgctcg cacgcacgct gatgcctttc
    27481 tggcataaaa actctaccgc gtttcgacct aagccgctgg tggcgccggt aaccagtacc
    27541 ttcatatcaa tccactgttg ttgagaaaat gacgtgcgca ttcttccgtg atttccccca
    27601 tgatgcaatg ggaaacatga aagaataacg caggttttgt cgattaatct gtgctttgtt
    27661 ctgccagtct ggcgatttgt tttgccattc cgcgaaaaat aaacagatgc gcggggatca
    27721 tcaataacca gtaaaacagc cccggcatac cgtgcggatg ccagaaagcg cggacatcga
    27781 tagtacgata gtcaccttta tcttccaggg taaaacacag tcgtcccagc cccggcgctt
    27841 tcatgccaaa taacaacgta agttgctttt tcggttcaac gacaatcact ttccagctat
    27901 ccaccgcatc gccggtctgt aaatattcgc gctccgggcg gcctttcgcc agattatgac
    27961 cgatcgcgcg gtccatcaac gcccgtgtct gccacaaaat attgccaaag aaataacgct
    28021 ctttaccgcc gatttggttc accaccagcc ataaagccgc aaggctggcg gacgttttaa
    28081 cggtaaaccc cgcctgtttg gcgaaataac cgtactccgg tcgccagcgg gcaaaggcct
    28141 gagcgtcgta gccccagtcg ctggagttga ccagtttttc ttcctctttc aacgtgctac
    28201 gtaccgcgtc atcgaaagcg atcagccgtt gtgggatgag tgcacgtagc gcggtatcat
    28261 ctgccagcag atcgtgtttc agcccctgaa tcaacgccct ggcggtggtg ggcggtacgg
    28321 aagtaatcac attgagaaac cacaccgaaa tccagcgggt ggggaggggg atggggatca
    28381 accagcggcg cttaccgctc accgccataa aatgttcaaa ctgttgctga taactgagca
    28441 cctctggtcc ggcggcttcg aagatgcggt gttcgttggc tggatggtct aacagcgcca
    28501 ccagatagtg cagcaagttt tccagcgcga tgggcgtggt gcgtgaacgt acccagcgtg
    28561 gcggcgttaa cactggcagg ttgtagacca tatcgcgcat gacttcgaac gccgctgaac
    28621 ctgcgccaac gataattccg gcccgaagtt cggtcactgg tacattcgct tcacgaagaa
    28681 tgtccgccgt agcctgacga gctcgcagat gatccgactg ctcatgtggc ggggcctgca
    28741 acgaactgag aaagattaat tgcttaactg gtacttcacg tagcgcatcg cggacgttga
    28801 gagccagctg gcgctcctga gcgataaaat cgccgccttc gcccatgctg tgcaccagaa
    28861 aatagacggt atcgatatcc tgcaacaggg ccggaaggtt atccggccag ctgagatcga
    28921 ctttatggca actgacgttt gccagttgca gctttgcaag cctgtcgaca tgacgtgccg
    28981 ccgctaggat ctgatgccct tgctggctga gtgtgcgcac cagatgctga ccaatgtagc
    29041 cactggcacc gagaactaaa atgcgttgcg gcacgtctct ctccttaacg cgccaggaat
    29101 gcgcgccagt gggcggcgac ttccgccagt tgttcgcgcg agacgtcaag atgcgtcacc
    29161 aggcggacaa tcggcgaggc gttaatcagc acgtttctcg ctttcatgta ttcgcctaac
    29221 gcggcagcat tttcttcccc gacgcgaaca aacagcatat tagtgtcctg acgcatcaca
    29281 tccgcgcctg cttcacgcag ttgctccgcc atccaggcgg cgttgtcgtg atcttcctgc
    29341 aaccgtgcga cgttattttc cagggcatac atcccggcgg cagccagaat cccggactga
    29401 cgcatcccgc caccggccat tttccgccag cggatggcac gtttaatgta atcacgatta
    29461 ccgacgagta atgaaccgac tggcgtccca agacctttcg acaggcaaat ggtgaacgaa
    29521 tcacaatatt gcgtgatctc tttcagttcg cagccgtaag ccaccacggc attaaagatg
    29581 cgcgcaccgt caacatgcag cgccagattg cgctcgcggg taaattccca tgcttctttc
    29641 aggtattcac gcggcagcac tttgccgttg tgggtgtttt ccagactgag taatttggtg
    29701 cgggcgaaat ggatatcgtc gggtttgatt ttcatcgcca ctttatccag cggtagcgtg
    29761 ccgtcggcag ccgcgtcgat gggttgcggc tgaatactgc ccagcaccgc tgcgccaccg
    29821 gcttcaaaca gatagttatg cgcggcctga ccgacaatat actcttcgcc acgttcgcag
    29881 tgactgagca gagcgaccag gttggcctga gtgccggtag gcagaaaaat ggcggcttct
    29941 ttaccggaaa gctctgctgc atagtcctgc agagcattaa cggtagggtc gtctccgtaa
    30001 acgtcgtccc caaccggggc ggccatcatc gcttcgagca tggcgcggct cggtcgggta
    30061 acggtatcac tgcgtaaatc aatcatggca tgtccttatt atgacgggaa atgccaccct
    30121 ttttacctta gccagttcgt tttcgccagt tcgatcactt catcgccgcg tccgctgata
    30181 attgcgcgca acatatacag gctaaaacct ttggcctgtt cgagtttgat ctgcggtgga
    30241 atggctaatt cttctttggc gaccaccaca tccaccaaca ccggaccgtc gatagagaag
    30301 gcgcgttgca gggcttcatc aacttcagag gctttttcta cacggatacc cgtaatgccg
    30361 cacgcttcgg caatgcgggc aaagttggtg tcgtgcagct cagtaccgtc tgtcaggtaa
    30421 cctccggcct tcatctccat cgccacaaag cccagcacgc tgttattaaa gacgataatt
    30481 ttcaccggca gtttcatctg cactactgag aggaaatcgc ccatcaacat gctaaaaccg
    30541 ccatcgccgc acatggcgac cacctgacgt tccggctcgg ttgcctgcgc acccaacgcc
    30601 tgcggcatgg cgttagccat cgaaccgtgg ttaaacgaac ctaacaggcg acgcttgccg
    30661 ttcattttca gataacgtgc cgcccatacc gttggcgtgc cgacgtcaca ggtaaagatg
    30721 gcgtcatcgg cggcaaaatg actaatttgc tgcgccagat attgcgggtg aatggctttc
    30781 tcgctcggtt tagctaaatc gtccagccct ttgcgggcat cgcggtaatc ttccagccct
    30841 ttgcgggcat cgcggtaatc ttccagcgct ttatccagaa acttgcgatc ggttttttct
    30901 tccaccagtg gcagcaatgc gcgcagggtt gatttgatat cgccgaccag cgccatatct
    30961 accttgctat gcgcgccgat gctggctgga ttaatatcaa tctgaataat tttggcatcg
    31021 gtcgggtaga acgcacggta ggggaactga gtaccgagta gcactaacgt gtcggcgttc
    31081 atcatggtat ggaaacctga cgagaatccg attaacccgg tcattccaac atcatacgga
    31141 ttatcgtatt cgacatgctc tttaccgcgt agggcatgaa caataggtgc tttaattttc
    31201 ccggcaaact caactaactc tttatgcgcc cccgcgcagc cgctgccaca catcagggcg
    31261 atattgctgg aataacgcag cagttgcgcc agtttgcgta actcttcttc ttccggcatc
    31321 acgactggct gtggcgcatg ataccagtgc gtggttgccc cttctggcgc aggttttaac
    31381 gccacgtcgc ccggtaatac aacaacggaa acgccacggt taagcaccgc tttgcgcata
    31441 gcaattgcca gcacttgtgg gatctgctcc gggctggaaa ccagctcgca atagtgacta
    31501 cattcgcgga atagctcttg tgggtgggtt tcctggaaat agccgctgcc aatttcgctg
    31561 gagggaatat gagcggcaat cgccagtacc ggaacgttat tgcggtggca atcgaatagg
    31621 ccgttgatca agtgcaggtt gcctgggccg cacgatccgg cacagaccgc cagttctcct
    31681 gaaagttgtg cttcagcgcc agcggcaaac gccgccactt cttcatggcg ggtggacatc
    31741 cactcgatgg tgcccatgcg attaagactg tcgctaagac cgttcagaga gtcgcccgtg
    31801 actccccaga tgcgtttcac gcctgccgat tcgagtgttt tggcgatata agctgcaacc
    31861 gtttgtttca tggttctcca tctcctgaat gtgataacgg taacaagttt agttcatctg
    31921 acggaggggg aagggatggg agagaaagga ggcactaacg gttaaatagc ccgatgaaag
    31981 gaatatcatc gggcataagg cgattatgcg agaaccaaat ccccctgcgg atggcaggag
    32041 caggccagta cgtaaccttc agcgatttcg gcgtcggtca gcgtcattgt actgctcacc
    32101 gtatattcac cggaaaccac ttttgtctta cagcagccgc aaacacccgc acggcaggca
    32161 gcgacaaccg gaacgttatt gctttccagc gcctccagta gcgtggtgcc aaccggggcg
    32221 taaaattctc gtgccggttg cagtttggtg aatttcagac cgctggtcgc cgcttctgct
    32281 actggggtga agaatttctc tttaaagaaa cgcgtcacgc cgagcgcttt cacttcctgc
    32341 tctacccaat ccatatacgg agccgggccg caggtcatca cggtacgtga agctaagtca
    32401 ggtacatctg ccagcagttc gcgagtgagg cgaccagcga taaagccttc ggtaacgtta
    32461 ttttctgcca ccagcgttac cggatagtta cgccactcat cggcgaaaat aacatcctgc
    32521 ggcgtacgca cgttgtagat cacccgcaca tcggcctgtg gacggttctt cgcaagccag
    32581 cgacgcatcg acataatcgg cgtgacgccg cagcctgccg ccagcaacag gaatttatct
    32641 tctgctttat cgtcgcaggt aaattccccc atcgcgtccg aaagccagag ataatcaccg
    32701 cgttttacat cgcgcgtcag ccactgggag ccgacaccgt catcaatccg ccgcacggtc
    32761 agggtgatat attcactcac gcctggcgtg gaggaaatgg tgtaagcacg cagcgtttcc
    32821 gctgagtttc gcacgctgac cagtgcatat tgcccggcgc gatatgggta gtgatcgtgg
    32881 caaatcaggg aaatcgtcca cacatccggc gtttcttgcg taatgtgatg aacctgtatc
    32941 cgccacgggc attgattcgt tggcatcgtc atcgacaaac tccttacgcg ttcaacagtt
    33001 gcttcatgtc ttcttcaaca gtggtgatag aacgcaggcc gaatttctcg ttcagcaccg
    33061 ccagcaggtc tggtgtcagg aaaccaggtg cagtcgggcc ggtgacgata tttttcacgc
    33121 ccagagaaag cagcgtcagc agaatgacaa tcgctttctg ttcaaaccag gagagcacca
    33181 gcgacagcgg cagatcgttc acaccacagc ccagtttctc tgccagagtg acagccagaa
    33241 taattgctga gtaagcatcg ttacattgac ctgcatctac cagacgcggc agaccttcga
    33301 tatcgccaaa ctcaagtttg ttaaagcgat atttaccaca ggcgagggtc aggatcaggc
    33361 agtcatccgg cacgctggtg gcgaaatcgg tgaagtagtg gcgctcgccg cgtgcgccgt
    33421 cacagccacc aagcaggaag atatgacgca gtttttcacg gctaaccaga tcaatcagcg
    33481 tatcggctgc accaagcagc gtctggcgac caaagcccac ggtgatcagg tgaggaattt
    33541 cgctgtacgg gaagcccgcc atttgttgcg cctgggcgat gaccgcagag aaatcttcgc
    33601 catccagatg acgcacgcca ggccagccaa cgatgctgcg ggtccagata cgatcgtcat
    33661 aagcgcctac ggttgggtcg atgatgcagt tcgaggtcat cacgatgggg ccagggaaac
    33721 gagcgaactc cacttgctga ttctgccagc cgctgccgta gttaccgacc agatgcttga
    33781 atttacgcag ctccgggtag ccatgcgcag gcagcatttc gccgtgggtg tagacattaa
    33841 cgcccgtgcc ttcggtctgt tccagcaggt tgtagagatc tttgagatcg tgaccggaaa
    33901 tcagaatgca tttacccgcc gtcgctttga cgttgacctg ggttggcgtc gggtgaccgt
    33961 atttaccggt ttcgcctgca tccagaatgc tcatcacttt gaagttcatc tggccgattt
    34021 ccattgaaca ctcaagaagc gcgttcatat cggcaggcca ggtccccagc cacgccatga
    34081 ttttatggta ctgggcataa atatcgttgt cgtattgacc gagaacatgc gcgtgttcca
    34141 tataggccgc cgcacctttc aggccataca ggcacagcag acgcaggccg agaatgtttt
    34201 cgccaatcgc cgctttatct ttgttagggg taaattctgc tgcctgacgt tgcagctcgc
    34261 cgagatcatc gctcaccagt tgcaggtcag ccatcgggtt atcgacgcgc gcgttggcat
    34321 ctacagccag gcattgtgct ttcagcgcct cgcgcaaggc aatcgcttca cgagcgtagc
    34381 cgacaatacg cggagaatcg aagttaacgt tggtcagggt tgagaaaaag gcacgtggcg
    34441 cgaagctgtc aacatcgtgg ttgatgatgc cgtattcacg cgcttttacc gcccaggcag
    34501 aaagcccttg cagcgccgcg atgagtaaat cctgaaggtc agaagtttcc gccgttttac
    34561 cacacatccc ctgcgcgtat gagcagccgt tttctgccgg agtacggata gtttgttcac
    34621 attgcacaca aaacatgatc acacctttta aagttatatt taatatacat gtttaagggt
    34681 aagacgctta acgcggggat aaaagggatt tttcatgcaa ctttaaggga gattgattta
    34741 gcgcaatttt ggcggcaggg aactaccgcc agagaggtat tacgcagaga aaaaggcgat
    34801 gaggatcggc actaacaggc taagaataaa accgtgaaca attgccgccg ggaccatatc
    34861 cagcccgcca gtacgttgaa gaacgggcag ggtgaaatcc attgatgtgg caccgcataa
    34921 gcccagtgca gtagagcggc tgcggcgaat cagcccaggg atcaacataa tagcaatcag
    34981 ttcacgggcc agatcattaa aaaacgccgc gctcccgatt accggaccaa aagattcggt
    35041 caataaaata ccggaaagag aataccagcc gaaaccggag gccattgcca gcgcggtatt
    35101 gatggggaga tcaagaataa aggcgttaat taaaccacca attaatgaac tgacaaccac
    35161 caccacggcg acaatcattc cccggcgatt aaggacaatc tgctttaagg tcatgccatt
    35221 attgcgcaac tgaataccaa cgaggaaaag tagcaaaatt aacgtgtatt cactggcttc
    35281 ggtcgcgtgt tgtaagaaag ccagtccact cagaccaatg gcaaaaccaa tcactactac
    35341 gccgcacagt tttagcgact ccagcgccat cgcaatacgc gacgggagtt tttcttgctg
    35401 atggtggttg cgccacggca ggcctcgctc cagccacatc agggcggcaa tattacacag
    35461 taaaataacg gtaatactga cggcagaata atgcagaatc gccaacaggt tactggcgag
    35521 gttatcgaga aacgccagac tgatacccat aaaaaagaga ataaggtaaa ccatccagct
    35581 taatagctga ttaatagctt ttaacgcagc ttgttggcga agcggaatga ggtaacccac
    35641 aatcagggga accagaatga ttaacagccc agaaaacatg aaaatccagt ccttgcaaag
    35701 aagaagtcga aatgcgcgat gacacactac tgaaagcgga agggcgagta aagttgcaat
    35761 taaaaggaaa tgttatgcat cagaggggg
//
LOCUS       DAJTUI010000033        34967 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTUI010000033 DAJTUI010000000
VERSION     DAJTUI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.033"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..783)
                     /locus_tag="OO464_001779"
     CDS             complement(<1..783)
                     /locus_tag="OO464_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6226952.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTR"
     gene            875..961
                     /locus_tag="OO464_001780"
                     /pseudo
     CDS             875..961
                     /locus_tag="OO464_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1366..1842)
                     /locus_tag="OO464_001781"
     CDS             complement(1366..1842)
                     /locus_tag="OO464_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6226953.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1901..3190)
                     /gene="bioA"
                     /locus_tag="OO464_001782"
     CDS             complement(1901..3190)
                     /gene="bioA"
                     /locus_tag="OO464_001782"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6226954.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3277..4317
                     /gene="bioB"
                     /locus_tag="OO464_001783"
     CDS             3277..4317
                     /gene="bioB"
                     /locus_tag="OO464_001783"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6226955.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4314..5468
                     /gene="bioF"
                     /locus_tag="OO464_001784"
     CDS             4314..5468
                     /gene="bioF"
                     /locus_tag="OO464_001784"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6226956.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5455..6210
                     /gene="bioC"
                     /locus_tag="OO464_001785"
     CDS             5455..6210
                     /gene="bioC"
                     /locus_tag="OO464_001785"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6226957.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6203..6880
                     /gene="bioD"
                     /locus_tag="OO464_001786"
     CDS             6203..6880
                     /gene="bioD"
                     /locus_tag="OO464_001786"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6226958.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7459..9480
                     /gene="uvrB"
                     /locus_tag="OO464_001787"
     CDS             7459..9480
                     /gene="uvrB"
                     /locus_tag="OO464_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6226959.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9672..10580)
                     /gene="yvcK"
                     /locus_tag="OO464_001788"
     CDS             complement(9672..10580)
                     /gene="yvcK"
                     /locus_tag="OO464_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6226960.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10845..10989
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10977..11966
                     /gene="moaA"
                     /locus_tag="OO464_001789"
     CDS             10977..11966
                     /gene="moaA"
                     /locus_tag="OO464_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6226961.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            11988..12500
                     /gene="moaB"
                     /locus_tag="OO464_001790"
     CDS             11988..12500
                     /gene="moaB"
                     /locus_tag="OO464_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6226962.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12503..12988
                     /gene="moaC"
                     /locus_tag="OO464_001791"
     CDS             12503..12988
                     /gene="moaC"
                     /locus_tag="OO464_001791"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6226963.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            12981..13226
                     /gene="moaD"
                     /locus_tag="OO464_001792"
     CDS             12981..13226
                     /gene="moaD"
                     /locus_tag="OO464_001792"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6226964.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13228..13680
                     /gene="moaE"
                     /locus_tag="OO464_001793"
     CDS             13228..13680
                     /gene="moaE"
                     /locus_tag="OO464_001793"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6226965.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13817..14521
                     /locus_tag="OO464_001794"
     CDS             13817..14521
                     /locus_tag="OO464_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6226966.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14726..15439
                     /locus_tag="OO464_001795"
     CDS             14726..15439
                     /locus_tag="OO464_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6226967.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15475..16431)
                     /locus_tag="OO464_001796"
     CDS             complement(15475..16431)
                     /locus_tag="OO464_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR6226968.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16431..17672)
                     /gene="clsB"
                     /locus_tag="OO464_001797"
     CDS             complement(16431..17672)
                     /gene="clsB"
                     /locus_tag="OO464_001797"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6226969.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17669..18430)
                     /locus_tag="OO464_001798"
     CDS             complement(17669..18430)
                     /locus_tag="OO464_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6226970.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18563..18973
                     /locus_tag="OO464_001799"
     CDS             18563..18973
                     /locus_tag="OO464_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6226971.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18935..20041)
                     /locus_tag="OO464_001800"
     CDS             complement(18935..20041)
                     /locus_tag="OO464_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6226972.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20052..21185)
                     /locus_tag="OO464_001801"
     CDS             complement(20052..21185)
                     /locus_tag="OO464_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6226973.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21178..22914)
                     /locus_tag="OO464_001802"
     CDS             complement(21178..22914)
                     /locus_tag="OO464_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6226974.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22907..23902)
                     /gene="hlyD"
                     /locus_tag="OO464_001803"
     CDS             complement(22907..23902)
                     /gene="hlyD"
                     /locus_tag="OO464_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6226975.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23905..24576)
                     /gene="cecR"
                     /locus_tag="OO464_001804"
     CDS             complement(23905..24576)
                     /gene="cecR"
                     /locus_tag="OO464_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6226976.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24805..25992
                     /gene="rhlE"
                     /locus_tag="OO464_001805"
                     /pseudo
     CDS             24805..25992
                     /gene="rhlE"
                     /locus_tag="OO464_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26199..26681)
                     /gene="ybiA"
                     /locus_tag="OO464_001806"
     CDS             complement(26199..26681)
                     /gene="ybiA"
                     /locus_tag="OO464_001806"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR6226977.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26801..28951
                     /gene="dinG"
                     /locus_tag="OO464_001807"
     CDS             26801..28951
                     /gene="dinG"
                     /locus_tag="OO464_001807"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6226978.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28979..29941
                     /gene="ybiB"
                     /locus_tag="OO464_001808"
     CDS             28979..29941
                     /gene="ybiB"
                     /locus_tag="OO464_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6226979.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30082..31167
                     /gene="hcxB"
                     /locus_tag="OO464_001809"
     CDS             30082..31167
                     /gene="hcxB"
                     /locus_tag="OO464_001809"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6226980.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31396..31656)
                     /locus_tag="OO464_001810"
     CDS             complement(31396..31656)
                     /locus_tag="OO464_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6226981.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31921..32187)
                     /gene="ybiI"
                     /locus_tag="OO464_001811"
     CDS             complement(31921..32187)
                     /gene="ybiI"
                     /locus_tag="OO464_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR6226982.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32261..32938)
                     /gene="ybiX"
                     /locus_tag="OO464_001812"
     CDS             complement(32261..32938)
                     /gene="ybiX"
                     /locus_tag="OO464_001812"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6226983.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32980..34967)
                     /locus_tag="OO464_001813"
                     /pseudo
     CDS             complement(32980..34967)
                     /locus_tag="OO464_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8287 a         8969 c         9445 g         8266 t
ORIGIN      
        1 gcgagtcgac aatggattgt tataaatatt aacatctgca taaatgtgca gattcatgat
       61 actgtctggc aatgtgttca gctgattgtt agggatatat agtgattgca gacgttgtgg
      121 aagttcgggc agtctgtgta gttggttttc cccaacgttt aaaagcctca gattttgagg
      181 aaggggcggt agctcttgta atctgttatc aaaagcactt agttcctgta gttcacatgg
      241 taattctggt aaggcagtaa gctggttgtt gccaacagaa agtttttgta gagccggggg
      301 aagatcaggt agcgtctcca ggctattgag aagggctgag agtgactgca aggaagatgg
      361 aagagaaggc aaacatgtta aaactctgtt aagtgaaaca tcaagagcga ccaggtgagg
      421 cggaagagcc gggagcctgc tcagtctgtt atcgctggct ttaagtacag ttaaggacgg
      481 agggagttct ggcagagagc gtagctcatt gctagagatg ttaagttctt gtatgtgcgg
      541 gggcaggtat gggagagagc gtaatctgag taaacttaaa ttgagggctg gctcttgaaa
      601 agccagacat attttcagta atcgaactgc ctgtgttcgg tcttctgttg cagcaccttc
      661 cttggcccag ttatcccaga tgcggtgata atccgtgata ttttgctctt ctacggataa
      721 acgggatata catcctaaac cacaaaagga gcggaaaaaa gaaggattgt gggcaggttt
      781 catttattat tgatcctcat tgtttatata ttaatatatt cgtgcaaatg gagtgaggat
      841 tctttaaaaa aacgcgcctg taaatagtta agaacaggaa gaactgttgg ggatgtggct
      901 ggccgaaaat gaaggggtga agttctggct caatgtgctg actgaactga aaaaacgcgg
      961 ttggtcgccc taccttttca tcaagccagt agtctgccca ccacagcatc atttctctgc
     1021 gcttatcgag atactgagca tggttgtaaa ttcagctaac ccagtgcgtt gacaaaaaat
     1081 tagcgcaaga ggacaaaaaa tcaccttgcg ctaatgctct gtatcaggtc actaatacca
     1141 tctaagtggt tgattcatag tgactggata tgttgtgttt tgcagtatta tgcagtctat
     1201 tttttaagct aaatgtatta taatgcattg atattaatga tttttactgt ttctcggtca
     1261 gcttttttat actaacttga gcgaaacggg aaggtaaaaa gacaaaaagt tgtttttaat
     1321 acctttaagt gataccagat ggcattgcgc catctggcag agtgattaac taaacatcgc
     1381 ggtaatcgat gcgcttgcca gagagtggaa atgaacgtta aacccgacca tcgcgccgct
     1441 ggctccttca tcgacatcaa tacgctctac atccagcgcg tgaacggtaa aaatgtagcg
     1501 atgggtttcg cctttcggcg gcgctgcgcc atcgtatccg gttttaccaa aatcggtacg
     1561 cgtctgcaaa acgccgtctg gcattgctac cagaccagag ccaaaccctt gcggtaatac
     1621 gcgggtatca gcgggtaaat taacaactac ccagtgccac cagccggagc cggttggcgc
     1681 atccgggtcg tagcaggtga caacaaaact tttcgttccc gcaggaacat catcccacgc
     1741 cagatgcggt gaaatattat cgccatcgta acccatgccg ttaaagacat gacgatgcgg
     1801 caacttatcg ccatcgcgca gatcgttact gatgagtttc atgaaccctc ctttcttgtt
     1861 tgcagaaagt gtagccagaa accctcacgc tgacttcccg ttattggcaa aaaaatgttt
     1921 catcctgtac cgcgcggtta accgctgcgg tcagacgctg caactgttgc gggagaataa
     1981 tatagggcgg catcaggtaa atcagtttgc caaaaggccg gatccatacc ccctgttcga
     2041 caaagaattt ttgcagcgcc gccatattca ccggacgagt ggtttcgacc acaccgattg
     2101 cccccagtac gcgcacatcg gcaaccattt cggcatcacg ggctggtgcc agttgctcgc
     2161 gcagctgcac ttcaatagcc gccacctgct gctgccattc gccggattcg ataatcgcta
     2221 ggctggcgtt tgctgccgcg caggccagcg gattgcccat aaaagttggc ccatgcataa
     2281 agcagccggc ttcgccgtta ctgatggttt ctgcaacctc gcgcgtggta agtgtggcgg
     2341 aaagggtcat tgtgccgccg gttaaggctt taccgaggca caaaatgtcc ggcgcgattt
     2401 ctgcatattc acaggcaaac agtttgccgg tacgaccaaa tccggtggcg atctcgtcgg
     2461 caatcagcaa gataccttcg cgatcgcaca ttttgcggat tcgttttaac cattccggat
     2521 ggtacatgcg catcccgcct gcgccctgga caatcggctc aatgatcacc gctgcgattt
     2581 catgacgatg cgccgccatc aggcgggcaa aaccaaccat atcgcgttca tcccattcac
     2641 catccatgcg gctttgcggg gcgggagcaa acaagttttc tggcagatag cctttccaca
     2701 ggctgtgcat tgagttgtcc ggatcgcaca ccgacatcgc gccaaaggta tcgccatgat
     2761 aaccattgcg gaaggtcaga aaacgctggc gcgcttcgcc tttggcttgc cagtactgca
     2821 acgccatttt catcgccact tccaccgcta cggaaccgga gtccgcgaga aaaacgcact
     2881 ccagcggttg cggcgtcatt gccaccagtt tgcggcatag ctcgatggcg ggcgcatggg
     2941 tgataccgcc aaacatcaca tgcgacatgg catcaatttg cgtcttcatc gccgcattaa
     3001 gctgcgggtg attgtagccg tggatcgccg cccaccagga cgacataccg tcaaccaggc
     3061 gtctgccgtc agacaaaatt agctcgcaac cttcggcgct cgccaccgga taaaccggca
     3121 gaggggaggt catggatgtg tatgggtgcc agatatggcg ttggtcaaag gcaagatcgt
     3181 ccgttgtcat aatcgacttg taaaccaaat tgaaaagatt taggtttaca agcctacacc
     3241 gaattaacaa caaaaaacac gttttggaga agccccatgg ctcaccgccc acgctggaca
     3301 ttgtcgcaag tcacagaatt atttgaaaaa ccgttgctgg atctgctgtt tgaagcgcag
     3361 caggtgcatc gccagcattt cgaacctcgt caggtgcagg tcagcacgtt gctgtcgatt
     3421 aagaccggag cttgtccgga agattgcaaa tactgcccgc aaagctcgcg ctacaaaacc
     3481 gggctggaag ccgagcggtt gatggaagtt gaacaggtgc tggagtcggc gcgcaaagcg
     3541 aaagcggcag gatcgacgcg cttctgtatg ggcgcggcat ggaagaatcc ccacgaacgc
     3601 gatatgccgt acctggaaca aatggtgcag ggggtaaaag cgatggggct ggaggcgtgt
     3661 atgacgctgg gcacgttgag tgaatctcag gcgcagcgcc tcgcgaacgc cgggctggat
     3721 tactacaacc acaacctgga cacctcgccg gagttttacg gcaatatcat caccacacgc
     3781 acttatcagg aacgcctcga tacgctggaa aaagtgcgcg atgccgggat caaagtctgc
     3841 tcaggtggca ttgtgggctt aggcgaaacg gtaaaagatc gcgccggatt attgctgcaa
     3901 ctggcaaacc tgccgacgcc gccggaaagc gtaccaatca acatgctggt gaaggtgaaa
     3961 ggcacgccgc ttgccgataa cgatgacgtt gatgcctttg attttattcg caccattgcc
     4021 gtagcgcgga tcatgatgcc gacctcttac gtgcgccttt ctgccggacg cgagcagatg
     4081 aacgaacaga ctcaggcgat gtgctttatg gcaggcgcaa actcgatttt ctacggttgc
     4141 aaactgctga ccacgccgaa tccggaagaa gataaagacc tgcaactgtt ccgcaaactg
     4201 gggctaaatc cgcagcaaac tgccgtgctg gcaggggata acgaacaaca gcaacgtctt
     4261 ggacaggcgc tgatgacccc ggacaccgac gaatattaca acgcggcagc attatgagct
     4321 ggcaggagaa aatcaacgcg gcgctcgatg cgcggcgtgc tgccgatgcc ctgcgtcgcc
     4381 gttatccggt ggcgcaagga gccggacgct ggctggtggc ggatgatcgc cagtatctga
     4441 acttttccag taacgattat ttaggtttaa gccatcatcc gcaaattatc cgtgcctgga
     4501 agcagggggc ggagcaattt ggcatcggta gcggcggctc cggtcacgtc agcggttata
     4561 gcgtggtgca tcaggcgctg gaagaagaac tggccgagtg gcttggctat tcgcgggcgc
     4621 tgctgtttat ctctggtttt gccgcgaatc aggcagtcat tgccgcgatg atggcgaaag
     4681 aggaccgtat tgttgccgac cggcttagcc atgcctcatt gctggaggct gccagtttaa
     4741 gcccgtcgca gcttcgccgt tttactcata acgatgtcgc tcatctggcg cgactgcttg
     4801 cttccccctg tccggggcag caactggtgg tgacagaagg cgtgttcagc atggacggcg
     4861 atagcgcgcc actggcggaa atccagcagg taacgcaaca gcacaatggc tggttgatgg
     4921 tcgatgatgc ccacggcacg ggcgttatcg gggagcaggg gcgcggcagc tgctggctgc
     4981 aaaaggtaaa accagaattg ctggtagtga cttttggcaa aggatttggc gtcagtgggg
     5041 ctgcggtgct ttgctcaagt acggtggcgg attatctgct gcaattcgcc cgccacctta
     5101 tctacagcac cagtatgccg cccgctcagg cgcaggcatt acgtgcgtcg ctggcggtca
     5161 ttcgccgtga tgagggggat gcacggcgcg aaaaactggt gtcactcatt gcgcgttttc
     5221 gtgccggagt acaggatttg ccgtttacgc tggctgattc atgcagcgcc atccagccat
     5281 tgatcgtcgg tgataacagc cgtgcgttac aactggcaga aaaactgcgc cagcaaggct
     5341 gctgggtcac ggcgattcgc ccgccaaccg tacccgctgg tactgcgcga ctgcgcttaa
     5401 cgctaaccgc tgcgcatgaa atgcaggata tcgaccgtct gctggaggtg ctgcatggca
     5461 acggttaata aacaagccat tgcagcggca tttggtcggg cagccgcaca ctatgagcaa
     5521 catgcagatc tacagcgcca gagtgctgac gccttactgg caatgcttcc acagcgtaaa
     5581 tacacccacg tactggacgc gggttgtgga cctggctgga tgagccgcca ctggcgggaa
     5641 cgtcacgcgc aggtgacggc gttagatctc tcgccgccaa tgcttgcgca agcccgccat
     5701 aaagatgccg cagatcatta tctggcagga gatatcgaat ccctgccgtt agcgactgcg
     5761 acgttcgatc ttgcatggag caatctcgcg gtgcagtggt gcggtaattt atccacggca
     5821 ctccgcgagc tgtatcgggt ggtgcgcccc ggaggcgtgg tcgcgtttac cacgctggtg
     5881 cagggatcgt tacccgaact gcatcaggcg cggcaggcgg tggacgagcg tccgcatgct
     5941 aatcgctttt taccgccaga tgaaatcgaa gagtcgctga acggcgtgcg ttatcaacat
     6001 catattcagc tcatcacact atggtttgat gatgcgctca gtgccatgcg ttcgctgaaa
     6061 ggcatcggtg ctacgcatct tcatgaaggg cgcgacccgc gaatattaac gcgttcgcag
     6121 ttgcagcgat tgcaactggc ctggccgcaa caacaggggc gatatcctct gacgtatcat
     6181 ctttttttgg gagtgattgc tcgtgagtaa acgttatttt gtcaccggaa cggataccga
     6241 agtggggaaa accgtcgcca gttgtgcact tttacaagcc gcgaaggcag taggttatcg
     6301 gacggcaggt tataaaccgg tcgcctctgg cagcgaaaag accccggaag gcttacgtaa
     6361 tagtgacgcg ctggcgttac agcacaacag cagcctgcag ctggattacg caacggtaaa
     6421 tccttacacc ttcgcagaat ccacctcgcc gcacatcatc agcgcccaag agggcaggcc
     6481 gatagaatca ttggtaatga gcgccggatt acgcgcgctt gaacaacagg ctgactgggt
     6541 gttagtggaa ggtgctggcg gctggtttac gccgctttct gacactttca cttttgcaga
     6601 ttgggtaaca caggaacaac tgccggtgat actggtagtt ggtgtgaaac tcggctgtat
     6661 taatcacgcg atgttgactg cgcaggcaat acaacacgcc ggactgactc tggcgggttg
     6721 ggtggcgaac gatgttacgc ctccgggaaa acgtcacgct gaatatatga ccacgctcac
     6781 ccgcatgatt cccgcgccgc tgctgggaga gatcccctgg cttgcagaaa atccagaaaa
     6841 tgcggcaacc ggaaagtaca taaaccttgc cttgttgtag ccattctgta tttggttaaa
     6901 ttgcgagcga gatcgcgtct tcgattgact gcaatttaac caattaaatt ctaaaataat
     6961 cacgaaaaaa attttacttc cgcctcatgc ggcgaatgtg ggaattgccc aggcggcggg
     7021 ggataggggc tggagacagt tatccactat tcctgtggat aaccatgtgt attagagtta
     7081 gaaaacacga ggcaagcgag agaatacgcg gcttgcacgc gaattggcgt taaagacggc
     7141 tcaaagaaat atcttttatt ttttaactgg ttagataaat gcaatggcag tcactgaaca
     7201 ggcatctctt gccataaaac tgtcatcact catcttgaca aatgttaaaa aagccgttgc
     7261 tttggggata acccggtaag gctggagttt tatctcgcca cagagtaaat tttgctcatg
     7321 attgacagcg gagtttacgc tgtatcagaa atattatggt gatgaactgt ttttttatcc
     7381 agtataattt gttggcataa ttaagtacga cgagtaaaat tacatacctg cccgcccaac
     7441 tccttcaggt agcgactcat gagtaaaccg ttcaaactga attccgcttt taaaccttct
     7501 ggcgatcagc cagaggcgat tcgacgtctc gaagaggggc tggaagatgg cctggcgcac
     7561 cagacgttac ttggcgtgac tggctccggg aaaaccttca ccattgccaa tgtcattgct
     7621 gaccttcagc gcccaaccat ggtacttgcg cccaacaaaa cgctggcggc ccagctgtat
     7681 ggcgaaatga aagagttctt cccggaaaac gcggtggagt atttcgtctc ctactacgac
     7741 tactatcagc cggaagccta tgtaccgagt tccgacacct tcattgagaa agatgcctcg
     7801 gttaacgaac atattgagca gatgcgtttg tccgccacca aagcgatgct ggagcggcgt
     7861 gatgtgattg tagtggcgtc tgtttccgcg atttatggtc tgggcgatcc tgatttatat
     7921 ctcaagatga tgctccatct cacggtcggt atgattatcg atcagcgcgc gattctgcgc
     7981 cgactggcgg agctgcaata cgctcgtaat gatcaagcat tccagcgtgg tactttccgc
     8041 gttcgtggcg aggtgataga tatcttcccg gcagaatcgg atgacattgc acttcgcgtg
     8101 gaactgtttg acgaggaagt ggaacgattg tcgttatttg acccgctgac cgggcagatt
     8161 gtttccacta ttccacgttt taccatctac ccgaaaacgc actacgtcac accgcgcgag
     8221 cgcatcgtac aggcgatgga ggagatcaaa gaagaactgg ccgccagacg caaagtgctg
     8281 ttggaaaaca acaaactgct ggaagagcag cggctgaccc agcgtaccca gtttgatctg
     8341 gagatgatgg acgagctggg ctactgttcg gggattgaaa actactcgcg cttcctctcc
     8401 ggtcgtggac cgggtgagcc accgccgacg ctgtttgatt acctgcctgc cgatgggctg
     8461 ctggtcgtcg atgaatctca cgtcaccatt ccacaaattg gcggcatgta tcgcggtgac
     8521 cgggcgcgta aagagacact ggtggagtac ggcttccgcc tgccatcagc gctggataac
     8581 cgtccgctta agtttgaaga gttcgaagca ttagcgccgc aaaccatcta tgtttcggcg
     8641 acgccgggta attacgagct ggaaaaatcc ggcggcgatg tggtggatca ggtggtgcgt
     8701 ccaaccggat tgcttgaccc gattatcgaa gtgcggccgg tggcgacaca ggttgatgat
     8761 cttctttcgg agattcgtca gcgagcggca attaacgaac gcgtgctggt caccacactg
     8821 accaagcgaa tggcggaaga tcttaccgaa tatctcgaag aacacggtga gcgcgtgcgt
     8881 tatcttcact cagatatcga caccgtcgag cgtatggaga ttatccgcga cttgcgtctg
     8941 ggtgagttcg acgtattggt agggatcaac ttactgcgtg aaggtctgga tatgccggaa
     9001 gtgtcgctgg tggcgatcct cgacgctgac aaagaaggct tcctgcgttc cgaacgttcg
     9061 ttgatccaga ccattggtcg tgcggcacgt aacgttaacg gtaaagcgat tctctacggc
     9121 gataggagca ctccatcaat ggcgaaagcg attggcgaaa ccgaacgtcg ccgtgagaaa
     9181 cagcagaagt acaacgagga acacggcatt acgccgcaag gcttgaacaa gaaagtggtc
     9241 gatatcctgg cgctggggca gaacattgcc aaaaccaaag cgaagggcag aggaaaatcg
     9301 cgcccgattg ttgagccgga taatgtgccg atggatatgt tgccgaaagc gttgcagcag
     9361 aaaatccatg aactggaagg gctgatgatg caacacgcgc agaatctgga gttcgaagaa
     9421 gcggcacaaa ttcgtgacca gttgcatcag ttgcgtgagt tgtttattgc cgcatcgtaa
     9481 caggatagcg aagaagactg atgacaaacg gaaaacagtc tgatgcgcta cgcttatcag
     9541 gcctacattt tctccgcaat atattgaatt tgcgcggttt gtaggccgga taaggcgttc
     9601 acgccgcatc cggcataaac aacgcgcact ttgtcttcaa taggaagccg gaattttcct
     9661 tcggatttcc gttaacctaa agcctgtaac gccttttcca gcgcgttatg taacaactgg
     9721 cggtcatgac gatacggaat atcgctggcc tccagtacct cctggatcac aatccgctct
     9781 ttcaccgccg agacatccac ttttggcccg acgatgaccg catcaatgac ctttttacca
     9841 acatactgct ccataattgc cagcttgctt tccagcttca aattagccgc aggtaaactc
     9901 aactcacgcc ccagattgcc gatataaacc atcggcgctg gcgtgcggcg taatgcctgg
     9961 gcgatttcct tcagcagcag aattggcatc aggctggtat aaaaactgcc aggcccaata
    10021 atgatgagat ccgcttcatt gatagcgtga accgcctcac gcgttgcggg tacattaggc
    10081 gttaacaata actcttgaat cggcgtagtt aactggtcga tattgacctc gccgtaaact
    10141 tcatgcccct gatcgtcaat cgccatcaga tcaacaggat gctctgacat tggaatcaaa
    10201 tgcgtatcca ctttcagcag attacgaatt aaattgatgg cttccagagg ccgcacgcta
    10261 aggtgatcca gcgcctttaa catcaagttt ccgagattat gaccggaaag ttcgccattg
    10321 ccgccaaaac ggtattcaaa catcgcggag gcgacgttcg gttccgttat cagctggttg
    10381 aggcagttgc gcatatcgcc ccaagcaatg ccgccttctg aacggcgaat acgccccgtc
    10441 gagccaccat tatccgtggt ggtgacgata cccgttaaac gagaacccaa agacgaaagt
    10501 gatgagagaa cgcgtcccag tccatgccct ccgccgagag caacgacacg atcaagatca
    10561 gccagcgtac gattgcgcat atagtttcct ggagtcagat tatccgcgct acagtagcgc
    10621 aaattgcggg gaaacagcaa ttaacctgcc aatattgagg atacaaacta ttttcttcta
    10681 tctctaaagg acgatgcacg ctatgcctcc ctgatgatgt atatcaaagt aaaaccgcca
    10741 ttttccctta ttctgtagcg aaatagcacg atcatgacgc tatatacatg attacatagc
    10801 gaaagtgtgg atggtaaaaa tctcatttac acgctagtat cggcttaacc actaaacact
    10861 ctagcctctg cacctgggtc aactgatacg gtgctttggc cgtgacaatg ctcgtaaaga
    10921 ttgccaccag ggcgaaggaa gaaatgactt cgcctcccgt atctggaaag gtgtacatgg
    10981 cttcacaact gactgatgta tttgcgcgta agttttacta cttgcgcctg tcgattaccg
    11041 atgtgtgtaa ctttcgttgc acctactgcc tgccggatgg ctacaaaccg agcggcgtca
    11101 ccaataaagg ctttcttacc gtcgatgaaa ttcgccgggt tacgcgcgcc ttcgccagtc
    11161 tgggcaccga aaaagtgcgc ctgacaggag gagagccgtc tttacgccgc gactttaccg
    11221 atatcatcgc cgctgtgcgg gaaaacgacg ctatccgcca gattgcggtc accaccaatg
    11281 gttaccgtct ggaacgcgat gtggcgaact ggcgcgatgc gggacttact ggcattaacg
    11341 tcagtgtcga cagtttggac gcccgccagt ttcacgctat taccgggcag gataaattca
    11401 accaggtcat ggcagggatt gatgctgcat ttgaggccgg ttttgagaag gtcaaagtca
    11461 ataccgtgct gatgcgtgat gttaatcatc accagctcga cacctttctg aactggatcc
    11521 agcatcgccc tatccagctg cgtttcatcg aactgatgga aacgggcgag ggcagcgagc
    11581 tcttccgtaa gcatcacatc tctggtcagg ttctgcgtga cgagctactg cgtcgcggct
    11641 ggatccacca attacgtcaa cgcagcgacg gtcccgcgca agtcttttgc catccagatt
    11701 acgccggaga gattggcctt atcatgccgt atgaaaaaga cttctgcgcc acttgcaacc
    11761 gcctgcgcgt ttcctccatt ggtaaactcc atctctgcct gtttggtgaa ggcggcgtta
    11821 acctgcgcga tctgctggaa ggcgataccc agcaacaggc gctggaagcg cgtatttcag
    11881 cggcgctgcg ggagaagaaa cagacccatt tcctgcatca aaacaacacc ggtattacgc
    11941 aaaacttatc gtacattggc ggctaaaacg tcaaaaggag agatcagatg agtcaggtaa
    12001 gcactgaatt tatcccgacc cgtattgcta ttcttacggt ttctaatcgt cgcggtgaag
    12061 aagacgatac ctccggtcac tatctgcgcg attcggcgca agaagcgggc catcacgttg
    12121 tcgatgaagc catagtgaaa gaaaaccgct acgctattcg cgctcaggta tctgcgtgga
    12181 tcgccagcga cgatgtacaa gtggtattga ttacgggtgg tactggcctg acggaaggtg
    12241 atcaggctcc cgaagcgctg ctgccgttgt tcgaccgtga ggttgaaggt tttggtgaag
    12301 tgttccgtat gttgtcgttt gaagagattg gcacttccac gttgcaatct cgtgcggtag
    12361 cgggcgtcgc taacaaaacg ctgattttcg ccatgccggg ttcgaccaaa gcgtgccgta
    12421 ccgcatggga aaatatcatc gcgccgcagc tggatgcccg tacgcgtccg tgtaatttcc
    12481 atccacattt gaagaaataa gtatgtcgca actgacccat atcaacgccg ctggcgaagc
    12541 gcacatggtg gatgtctccg ccaaagcgga aaccgtgcgt gaagcgcggg cggaagcctt
    12601 tgtcaccatg cgcagcgaga cgctggcgat gattattgat ggtcgccacc acaaaggcga
    12661 cgtatttgcc actgcgcgta ttgccggtat tcaggcggca aaacgcactt gggatctgat
    12721 cccgctgtgt cacccgctga tgctcagcaa agttgaagtc aatttacagg ccgagccgga
    12781 gcacaatcgg gtgcgtatag aaaccttatg ccgcctgacc gggaaaaccg gtgtcgaaat
    12841 ggaagcgtta accgcggcct ccgtggcggc gctgaccatt tatgacatgt gcaaagcggt
    12901 gcaaaaagat atggtgattg gtccggtacg tttgctggcg aagagcggcg gcaagtcggg
    12961 tgactttaag gtggaagcgg atgattaaag ttcttttttt cgcccaggtg cgcgagttgg
    13021 tgggaacaga tgcaaccgaa gtggctgcgg attacccaac cgttgaagcg ttacgccagc
    13081 acctggctgc gcagagcgat cgctgggcgc tggcgctgga agatggcaaa ttgctggctg
    13141 ccgtcaacca gacgctggtg agttttgacc atccgctgac tgacggcgac gaagtagctt
    13201 tcttcccgcc ggtaaccgga ggttaagatg gcagaaacca aaattgttgt cggcccgcag
    13261 ccgttcagcg taggagaaga gtacccgtgg ctggcggagc gtgacgaaga cggtgcggta
    13321 gtcaccttca ccggaaaggt gcgcaaccat aacctgggcg acagcgtcaa agcattaacc
    13381 ctcgaacact atccggggat gactgaaaaa gcactggcag aaattgttga tgaagcgcgt
    13441 aaccgctggc cgctggggcg cgtcacggtg attcaccgca tcggggaatt atggccgggc
    13501 gatgaaatcg tttttgtcgg tgtcaccagt gcgcatcgca gcagtgcgtt tgaagccggg
    13561 cagtttatta tggattatct caaaacccgc gcaccgttct ggaagcgcga agccacgccg
    13621 gaaggcgacc gctgggttga agcccgggag agcgatctgc aggcggcaaa acgctggtag
    13681 ttttttgtta gccggataag gcaccgctgc gtccggcaat ttcaccgctc agtacatgcg
    13741 tttacgctgc gtcgatgcac agcctcatca ttttgcagta tccttaagat attccttata
    13801 tcttcaggag atcgtcatgg acagattccc acgttctgat tcaatcgtac aaccctgggc
    13861 tggcttgcaa acttatatgg cgcaagtcta tggctggatg accgttggct tgttgctgac
    13921 cgcatttgtt gcctggtatg cggctaattc cgcggccgtg atggagctgt tgttcactaa
    13981 ccgtgtcttt ttaatcggtc tgatcatcgc gcaattagca ttggttattg tgttatcagc
    14041 gatgattcaa aagctgagcg caggtgtaac gacgatgctc tttatgcttt attcggcgct
    14101 gacgggtctt acgctttcca gtatattcat tgtctatacc gctgcttcta tcgccagtac
    14161 tttcgtcgtt actgccggga tgttcggcgc aatgagcctg tacggttaca ccacgaagcg
    14221 cgatttaagt ggctttggca atatgctgtt tatggcgtta atcggcattg tgctggcatc
    14281 gctggtcaac ttctggctga aaagcgaagc attgatgtgg gcagttacct acatcggcgt
    14341 gattgtcttt gtcggattga cggcgtatga cactcagaaa ttgaaaaata tgggtgagca
    14401 gattgatacc cgcgacacgt cgaacctgcg caaatattcc attcttggtg cgttaacctt
    14461 gtatctggac ttcatcaacc tgttcctgat gttgttacgg atcttcggca accgccgtta
    14521 attctttttc accatcacta tcccggccat gtgtcgggat ttttttctta ctaaaaacga
    14581 cacttaacaa gtaccaggta taaaatatgc cttatgagcg agcggaatcg ttttccgtat
    14641 ccataaatac aaaagtattt tgtatgcgtg gttttaaatt tattgatggt gaatattaat
    14701 gttttttcta aggacaacta aaagaatgga atcatactcg caaaacagca ataaattaga
    14761 ttttcagcac gaggccagga tattaaacgg tatatggctc attaccgctt taggtttggt
    14821 ggcaaccgca ggactagcct ggggagctaa gtatctcgaa attacggcaa ccaaatatga
    14881 ttcaccacca atgtatgtcg ccatagggtt attattactt tgtatgtatg gcttaagtaa
    14941 ggatatcaac aagataaatg ccgccatcgc gggcgtaata tatctgtttt tactctcttt
    15001 ggtggcgatt gtcgttgcaa gtttagttcc tgtatatgcc attatcatcg tgttcagcac
    15061 tgcgggcgcg atgtttttaa tcagtatgct ggccggttta ttatttaatg ttgatcctgg
    15121 ttctcaccgt tttatcatta tgatgacgtt gacagggttg gccctggtaa tcatcgtgaa
    15181 tgcggcatta atgagtgaac ggcccatttg ggtaataagt tgcttaatga ttgtgttatg
    15241 gccaggcatt atctcgcatg gacgaaataa gctccttgaa ctggcaggaa aatgccatag
    15301 tgaagagttg tggagtccgg ttcgttgcgc ttttacaggt gcattaacac tctattacta
    15361 ttttatcggc ttctttggga tacttgccgc gatagctata acgcttgtct ggcaaaggca
    15421 tacgcgtttt tttcattagt cgtaaatgca gatactaaag aacttaacta aacttcacat
    15481 cgccgcttca tttttcgccc gtagcttctt cgcttggctt tccagtagca ggtaacagat
    15541 cagcgccagc agcagcggga taaagtaata caggacgcga taagcgagca gggcggcgat
    15601 aatgctccct ttcgaggtat gctccccagc cagtagtgcg ataaacaccg cttccagcac
    15661 accgatcccc gccggaatat gcacgatgac gccagcaata ctactaacca gtaacacgcc
    15721 cagtacaaag aaatagttca cgctttgacc aagtaacagc cagataatcg cccccattac
    15781 catccagtta acgctggaaa tcagcatctg agcgagggcg aatttccatg aaggcagcac
    15841 cagcttttgc cctttgatgg tcatatggcg gtgcttcgcg aaagcgcaaa accacaaata
    15901 aaccgcgata atcatcagta agccaatgcc gagaatacgc agcgtagttt gatcgacata
    15961 ccagtaatcc ggcaactcca ccacgcctgc ggtaaagata atccctgcca gtaaaatata
    16021 gcccagccag ttggtggtaa tactgagcga ggaaatccgc gtaatagtgc tgcccggtaa
    16081 ccctagtcgg gagtacaaac gatagcgcat accaatgccg ccgacccagg tactgagcgt
    16141 caggttgaag gcgtagcaga taaacgacac cagcatcacc tggcgcttcg ccagtttgtg
    16201 accgcagtaa aagcgggcga gcaggtcata gcagccgtaa atcagatagc tgacgaccac
    16261 cagcccgacc gcactaagca gcgcaacgcg attgtagtcg cggatgacct tccagacctc
    16321 ttcccagtcc acttttttgg cgtagaccac cagcaacaca atcaccgcga taaaaaacag
    16381 ccaggtgagg atcttctttg ctaagcgcca gcgcgggtgt gatttactca tcaggggttt
    16441 acccccgtgt tttcagtttc tacccgatcc tgcgtttcca ttgtcggttg tgcgggggga
    16501 tcaacctgcg tcagacgtgg tgtgtgtgcc ggaagccagc caaccagcgc cgggaagtgg
    16561 cgtaaaaagt ggaacgccag cacgcttttg gtcaggttcc accaggtgcg tttgggcagc
    16621 atggtttcat ccacctgctg acaatctgcg gcaataatgc cgttcagatt atcgcgcagc
    16681 gtctggttaa aatgacgatc gtggatgatg acatttgctt cgagattcag tgacaaactg
    16741 agcggatcga gattactgga ccctactgtc gcccagtgat cgtccatcaa tgccactttg
    16801 ccgtggagcg ggcggcggcg gtactcaaaa acctgaacgc cgcctttaac cagatagtta
    16861 tacagcaagc gcgcaccgac tctgacaatc ggcatatccg gttcgccctg aatgatcagt
    16921 ttgatccgca ccccgcgccg tgccgcttta cgcaaggcgt gtaaaaatcg atagccgggg
    16981 aagaagtagg cgttggcgat aatcacttcc cgctgcgcct gagtgagcat tttcagataa
    17041 tggcgttcaa tatcatcgcg atgttcttcg ttatcgcgcc agaccagcaa tacctgcgct
    17101 tctcccggct ggcggttctc ttccgctttg tgatgacgtc gccaccagcg tcgtgccgcg
    17161 ctctgtccag gcaggttttc cagctcaaac tggagaatat cttcgacaat cggcccttca
    17221 aggcgtaccg cgtaatcctg tttagcctct ggaccgtagc tggacatatg ctcggcggag
    17281 taattcagcc cgccaataaa ggcgatacgc gcgtcgatca ccacaatttt gcgatgcatc
    17341 cggcgaaaca cattggtgcg cataccaaaa aggcgagggc ggggatcgta gtagcggaac
    17401 actacgccag ctgccgtcag ttcattgaca aactcatcgc tgagatccgg cgaaccgtag
    17461 ccatccagca agacttccgc tttaaccccg cgttgcgctg ctgccagtag tgccgcatgc
    17521 agctgtttgc cgacgttatc ctcaaaccag ataaacgttt caagaatgat gcgttcttgt
    17581 gcctcgccaa tcgccttaaa caccgcggga taatattgct cgccgttttc cagcaactgg
    17641 atcttattgc cttcgcgcca gctacatttc ataaatgaat ctccgcactt aaaggggcat
    17701 gatcagaaag gtgtcgccat gtccgcagcg gcaacgcggt tggcgcgctg gcgctggcat
    17761 ttttgacgta gatcctgtcc agtcgtagta gaggaaattg caccggaaac gtgcgcgccg
    17821 ggcgtccgtg ggcgcgggta aaaatctcat ccagtccggc ctgcactttt aacggatgat
    17881 tagctttttg ccgccagtca ttgaaatcac ccgccaccaa taccggttcg ccgtccggta
    17941 gctcattcac ccattcggcg agcatcgcaa gctgcgcctg acggtgcgcc tcacgcaggc
    18001 ccagatgtac gcacatcaca tgaatcgctt ttccggtcat cggcggcaca atgcggcagt
    18061 agagcacgcc gcgcttttcc gcaccatcga ccgaaacatc gcgattctca taatgttcaa
    18121 tgggataacg cgacagtacg gcgttgccgt gatgcccttc cgggtatacg gcattgcgac
    18181 cgtaggcaaa atcgctccac atagtgtcgg cgagaaactc gtagtgcgag gtatcgggcc
    18241 agttttccac atgcagcgga tgaacttcgt gcgcgcccat cacttcctgc aggcaaacaa
    18301 tatcggcgct gacggtacgc acggcatcgc gaagttccgg caaaatgaag cgtcggttaa
    18361 acgcggtaaa gcctttgtga atgttgatgg tgagcacctt gaacgaaaat tgttgtgttt
    18421 gatcgggcat aattttcctg tctttgcctc ttatctcatt gaaatagtgt agtcggcgtc
    18481 acaaaaaggt gcggtcttac ggaattttcc gtaaagttcg gtactctgag taagtagaga
    18541 taaattcttc aggagagaag ccatgaagtg gcaacaacgt gttcgtgtcg caacgggtct
    18601 aagttgctgg cagattatgt tgcatttact ggtagtggcg ctgctggtgg tgggctggat
    18661 gagtaagact ctggttcacg tcggcgtggg attatgcgca ctgtattgtg tcacggtagt
    18721 gatgatgctg gtgtttcagc gccaccccga gcaacgctgg cgtgaggtgg cagacgtgct
    18781 ggaagagctg accacgacct ggtattttgg cgcagcgctg attgtgctgt ggctgttgtc
    18841 ccgcgtactg gaaaacaact ttttgctggc aattgcaggg ctggcgatcc ttgctggccc
    18901 ggcggtagtg tctttgctgg cgaaagataa gaagttacat caccttacgt ctaaacatcg
    18961 cgtacgccgc tgaccctgtc gtggccgtta tcaccagtag cggccacaaa ctattccaca
    19021 caatatccag actcgcatcc ttcaaataaa tctgcttggt aatgtccgta aagtggcgaa
    19081 tagggttaat ccacgtcaga ttttgcagcc acactggcat gttttccacc ggcgaaacgt
    19141 aaccggaaag gagaatggcg ggcatcataa aaacaaacac gccgataaac gcctgctgtt
    19201 gtgtcgagca gagtgatgaa atcaacagac cgaatcccac cagcgataaa ccgtaaatca
    19261 ccatcgtaaa gtagaacagc gctagcgatc cggcgaaggg gatttgatac gcccagatac
    19321 caatcgccag cacaatggtg gcctggaagg tggcgacaat taacgccggt acggctttgc
    19381 cgatgaagat ctgccaggtg gtgagcggcg aaaccagtag ctgatcgagc gtaccttgtt
    19441 cacgttcgcg ggcgacggaa agtgaagtga cgatcattac gccgatagtg gtgatcatgg
    19501 cgatcagcga cggcaccaca aaccatttgt agtcgagatt cgggttatac cagttgcgta
    19561 ccaccagctc gctgttgttg ggtttcggtt ttccttccag cagctcctgc tgataatttt
    19621 tgacgatctg ttgcaggtag ttggcggcaa tttgcgcact gttggagtta cgcccgtcga
    19681 ggatcaactg caacggcgcg gtctggaagg tatccagttt gcgcgagaag tcagccggga
    19741 aacgcaccag tagtaacgcc ttttgtgtgt cgatggtcgg gcggatctcc tgtgggcttt
    19801 tcagcagcag cacatgtgta aaggcgctgg cgcgggcaaa acgttgagta agttccaccg
    19861 aatgctcgcc gttatcttca tcgtagatgg cgatggtggc gttagtcact tccagcgtgg
    19921 cggcgaacgg gaacaggatc acctgaatta gcacgggtaa aatcagaatc gcgcgggttt
    19981 gtggttcgcg cagcaacgac tgcaactctt tgcggattaa cgtccataag cgatgaaaca
    20041 tgctcttctc cctaatccag ccgacgtttg gttttcagcc acgtcaggcc gataaacatc
    20101 accgccgaag cgatcaaaaa cagcacgttt accaccagca ccactggaat attcccggcg
    20161 aggaacaggc tttgcagggt gctgacgaaa taacgagcgg gaataatgta cgtcaccgcg
    20221 cggatcaccg cgggcatact gtcgatctga aaaataaagc cggaaagcat aatcgacggc
    20281 agaaaagcgg cgttcagggc gacctgagcg gcattgaact ggttgcgggt aatcgtggaa
    20341 atcagcagcc ccattcccag ggtactgagt aaaaacaggc tggagataaa aaacagaatc
    20401 agcagcgaac cgcgatacgg cacgccgaga ataaacactg acaccagcat acacagcaac
    20461 atcgccagca tcccgagaaa gtaataaggg atcagcttac acagcagcag ttccgtgcgg
    20521 gtaatctccg tagagagcag agcctccatg gtgccgcgtt cccattctcg cgccaccacc
    20581 agcgaggtga gaatcgcgcc gatgaccgtc atgataatgg tcaccgcacc cgggataata
    20641 aagtgctggc ttatcgccgc cgggttaaac cagtagcggg tttgtacatc aataagcggt
    20701 tcaaaagtct gcccgttgtc ctccgctcgc tgcatttgcc agatctgcca gatcccttcg
    20761 acatacccct gcacaaagtt agcggtattc ggctcactgc cgtcggtgat cacctgaatc
    20821 ggtgcggtgg cgttggcgcg ctccatctgt tcggcgaaat ccacgggaat aaccaccaga
    20881 ccgcgaattt tccccgactg cattttggcg atcagttcct gacggttatc gctgatggtg
    20941 gcgtcgatgt agggcgaacc ggtcatggtg tgggtgaaat ccagcgccgc ttcgctacgc
    21001 tgttccagca aaatcccgac ccgcagcttg ctggagtcga ggttaatgcc gtaaccaaaa
    21061 ataaacagca gtagcagtgg gatcactacc gcaatcagcc agctactcgg atcgcgaacg
    21121 atctgccgcg tctctttgac gcacagcgcc cgcacgcgat gccaggaaag gatcgggtta
    21181 ctcattgcta tgctccttat cccagtcgtg gatcaactga ataaaggctt gctccatggt
    21241 gggatcgggt tgctcatcgt tagctgactg tgctttcaaa tcgtccggcg taccgctggc
    21301 gattaatttc ccacggtaca ccaggccgat gcggtcgcaa tattccgctt catccataaa
    21361 gtgggtggtg accatcaccg tgacgccttt ctctaccatg ctgttgatgt gcagccaaaa
    21421 ttcacggcgg gtgagggggt caacgccgga agtcggttcg tcgagaaaga gaatatccgg
    21481 ttcatgcatc agcgaacagg ccagcgccag ccgctgttta aaacctaatg gcagttcatc
    21541 ggtggcgtgt gaggcaatac ttttcaggcc aaatgcctcg ctcatacggg agattttttc
    21601 gttctgcgcc cgaccgcgta agccatacac accagagaaa aagcgtaaat tctgttcgac
    21661 cgtcaggttg ccgtagagcg aaaatttttg cgccatatag ccgagatgct ggcgcgcttt
    21721 accggaactc tctttcagat ccatccccag caccagcgcc tggccggaag tcggcaccag
    21781 caaaccgcac atcatcttaa aggtggtcga tttacccgcg ccgtttggcc ccagcaaacc
    21841 aaaaatctcc ccacgtttaa cggcaaagtt gacgtgatcg gtggcggcaa aatcaccaaa
    21901 tttcttggtc agttctttcg cttcgatcac cgtctcgccg ggtgtgcctt ctaccgtatg
    21961 caatattgca cccagcggcg attctgaggt tccggcaccg cccagcaaat caataaacgc
    22021 atcttcaaaa cgcggcgtag tttcgttgat gttgatttcc ggcatcccgt cggcatggcg
    22081 aatatcgtct ggtgtggcct cttttttgag gatcagacgt accgatttcc cctgaatcat
    22141 gccatcgctg acctgcggca gtttcaaggc gcgttgcaac agtttgcggt tgccctcgtg
    22201 tggactggtc atcagaaagc tgcgtccggc catggtttgt gtcagggctt ttggttctcc
    22261 ctgatacagc aactcgcctt cgttcatcag taacacgtca cggcactgct cggcttcgtc
    22321 gagatacgag gtactccaga ggattaacat cccttcgccc gccagctcat gcaccatctg
    22381 ccacagttcg cgccgtgaga tagggtcaac gccgacgccg ggttcatcga gcagcaacac
    22441 tttcggttcg cccaccaggg tacaggccag gccgagtttt tgtttcatcc caccggagag
    22501 cttgcccgcc aggcgtccgg taaacggccc aagagacgta aactccagca ggcgagcaaa
    22561 agtttgctta cgtgcctcgc cggtgacgct gcgcaaatcc gcgtacagat tgaggttctc
    22621 catcaccgtg agatcttcat acagaccaaa tttctgcggc atatagccga gcacggcgtg
    22681 cagcgcgccg tcgtttttga tcggatcaaa gccaatcacc gtggcactgc cgctgtcggg
    22741 tttcagtaat cccgccaaca tccgcatcag tgtggtttta cctgcaccgt ccggccccac
    22801 caaccccgtc acataaccgg cgtgaatggt acaatcgagc ggcgcgacgg cgggcttgtc
    22861 catgcccgga aagcgttttt ccaggccgtt cagcgtgata acggcatcat tcatgtcctg
    22921 cctcgtcacc gaattgtacc gtcactggca ttccctggcg taacgcatca tcggcgtcgg
    22981 tcaccacaat acgcaggcga tagacgaggt cggtacgcag atccggtgtt tcgacggttt
    23041 tcggggtaaa ttcagcagtc ggcgaaacga aaccaatctg cccgtgatac ggcttgtccg
    23101 ggcgaccatc ggtataaagc agcactttgc gccccggctg ggcctggtca agattccgtt
    23161 catcaacata agcgcgcacc cacaccggac gcgttagtga aacggtaaac accgtgccac
    23221 cttcattgag aaccgtgcct ggctctaccg cgcgcgttaa cagcgtgcca tcagacggtg
    23281 ctatcaacgt tgagtcctgt aaattcagct ccgcctgcgc cagttgcgcc tgcgcctgtt
    23341 cgaggctggc tttcgcctga gcgatgtcct gttcacggtt accggaacgg tactgacgca
    23401 atttatcctg tgctgatttc agcgttgcct gtgcctggtc gcgcgaggag cgggcatttt
    23461 ccaggtcatt tgccgaaatg gtgcggcttt tccacagtcc ttgctggcgg ttatagaagt
    23521 tctgcgcata gtcataggcg gcttgcgcct gtttcaccgc tgcggcggcc tgagcgattt
    23581 cttcatcgcg atacccggca agcatcagat catactgcgc ctgtgccacc gagacgcccg
    23641 ctttcgcctg catcagggca atctcatacg gcttgtgatc cagttcgccc agcacctggc
    23701 ccgctttgat agcatcacct tcgtccaccg ccagcgattc aacgcgcccc ccaacacgga
    23761 aactaagatt caccgtacga atatccacgt tgccatacag cgtcaggccg ttatcctggc
    23821 ggctttgata ccaccagtag cctccggcaa ccacggcggc aagtaccact accgccagtc
    23881 cgatcacgac aggttttttc atcactacaa actcctttgc gataatcctt gcaaaatcag
    23941 gtcgatatga caggtcaccg tctggttgat cagttcggtc ttttcttcat cgaacgcggt
    24001 ccagccggta cgtaacagaa ttgtttcttt gccaagacgg aacgccagaa tctcaccaat
    24061 caacgcatgg gtatgaagga tcatgcgggt gtcattagcg tcgcagccgg tccaggcggc
    24121 aatcaggcgt gtcaggtggc tgtgtagcgg actaatcacc tgttcgtgca ccaggtggta
    24181 ggctgccgtg ggagagagct gctcacggga gataaacttg ctgaggttga cggtgtcatc
    24241 ctgggtgagc agcttaatca tgttcctgca ggcgcgaagg atcagttcac ggatggcagc
    24301 ccgatcaggc tgtggttggg cgaacaagcg ttcggcttcc tcggcatgcg gacggaactg
    24361 ctcgccaata aaatcggcaa tccactgggc gcaggcgagg tacaaatctt ctttcgaacc
    24421 gaagtagtag gtgatggcag cgatattctg cccggcctgg gcggctatct cgcgggtggt
    24481 ggcgttcatt ccatattcac caaactgcgc cagtgcggca gcaatcagct gttttttcgc
    24541 ctgttcaccc ttgattgtca tggcaggatt attcatcgca cagtccattc ttaatcaaat
    24601 gattgattaa gattatgact ccatatggga gttgtccagt atggctaaga attttagcta
    24661 cgccagtcac agggataatt tatgcgctgc gtcacaaaaa ctgctacact ccgctccctc
    24721 atgacattgt ggtttttgtc attttccttt tcagtatctc cctgaaaact acaccggtaa
    24781 cggtcggggc ggttcggagt agttatgtct ttcgattctt tgggtttaag ccctgatatc
    24841 ctgcgcgccg ttgccgagca gggttaccgt gaacccaccc ctattcagca gcaggcgatc
    24901 cctgctgtgc tggaaggccg cgacctgatg gctagcgccc agaccggcac cggcaaaaca
    24961 gcgggcttta cgctgccgct gttgcaacac ctgatcactc gccagccgca cgccaaaggg
    25021 cgtcgtccgg tacgtgcgct cattcttacc ccgacccgtg aactggcggc acagattggc
    25081 gaaaacgtcc gtgattacag caaatacctg aacattcgtt cgctggtggt gtttggcggt
    25141 gtcagcatta acccacaaat gatgaagttg cgcggcggcg ttgatgtgct ggtggcaacg
    25201 ccgggacgtt tgctggacct ggaacatcaa aatgcagtga agctggatca ggttgaaatc
    25261 ctcgtcctcg atgaagctga ccgcatgctc gacatgggct ttatccacga tatccgtcgc
    25321 gtgttaacaa aactgccagc gaaacgccag aacctgctgt tctctgcgac cttctctgac
    25381 gatattaaag ccctggcgga aaaactgttg cacaacccgc tggaaatcga agtggcacgc
    25441 cgcaataccg cgtctgatca ggtgacccag cacgttcact ttgtcgataa gaaacgcaaa
    25501 cgcgaattgc tgtcgcacat gattggtaaa gggaactggc agcaggtgct ggtgtttacc
    25561 cgtaccaaac acggcgctaa ccatctggct gagcagctca ataaagatgg catccgtagt
    25621 gtggcgatcc acgggaataa atcgcaaggt gcgcgtaccc gtgcgctggc tgattttaaa
    25681 tcgggtgaca ttcgcgtact ggtggcgacc gatatcgctg cgcgcggtct ggatatcgaa
    25741 gagttgccgc acgtcgtcaa ctatgaactg ccaaacgtac cggaagatta tgtccaccgt
    25801 atcgggcgta ccggtcgtgc ggctgctacc ggtgaagcgt tgtcgctggt gtgtgttgat
    25861 gaacacaaac tgctgcgtga tatcgaaaaa ctgctgaaaa aagagatccc gcgcattgcg
    25921 attccgggct atgagccgga cccgtcaatc aaagccgaac cgatccagaa cggtcgccag
    25981 caacgtggcg gcatcaggct gatgaacaaa cgcaaaactg cctgatgcgc tacgcttatc
    26041 aggcctacgt gaactctgca atatattgaa tttgcatgct tttgtaggcc ggataaggcg
    26101 tttacgccgc atccggcatt tcacaacaag cacttgtcag caatttgaga acacgggaaa
    26161 aaatttatct gttttaccgc ccataagcca ccgagacgtt acttctctat agccaattgt
    26221 tcgcgcaact ccattaaaag gtagcccagt cgattcttgc ccttaccatt accaccgtct
    26281 ccccagtaag catcgttttc cgtatgctca accagttttg cgggcgcggt tgccagcaag
    26341 agcacacgca gttctgcatg ctgttcgaat ttagcgcgaa gtgctttacg catcacctgt
    26401 tctttaaccg actcccagtt tttacgaagt ggcttggaac gattacgccc catgcgagcg
    26461 gcgaccatgg gggaagaaac ccgacggatc tcttcgcggt atttttcatc aaggaatttt
    26521 tgtgcctgaa aatagtgttc ggaggtgggc caggtattcc cgtcaacctt gatgggcctg
    26581 gcggcaaagt tagaaaaatc accgtagtca tcgctggtgc tgtagaaatt tatgatggtg
    26641 tcttgcatga cgtgttggat cctttgtgct cgaacgggca ttaaaccgca ttatgttggt
    26701 ggttattgcg agccgctttc cagaaacaga aaaaccatta cccctgaaaa ccgaaaaatg
    26761 ccacaatatt ggctgtttat acagtatttc aggttttctc atggcattaa ccgccgcgct
    26821 taaagcgcaa attgccgcct ggtataaggc gcttcaggaa cagatccccg actttattcc
    26881 ccgtgcgccg cagcggcaga tgattgcgga cgtcgccaaa acgctggccg gagaagaagg
    26941 gcggcatctg gcgattgaag cccccaccgg cgttgggaaa acgctctcct atttgatccc
    27001 cggcatcgcc attgcccgcg aagagcaaaa aacgctggtg gtgagtaccg ccaacgtggc
    27061 gttgcaggat cagatttaca gcaaagattt accgctgctg aaaaagatca ttcccgatct
    27121 taaattcact gccgctttcg ggcgtgggcg ctacgtttgt ccacgtaatc tgacggcgct
    27181 cgccagcact gaacccacgc aacaggatct gctggcgttt cttgacgacg aactgacgcc
    27241 aaataatcag gaagagcaaa aacgttgtgc gaagctgaag ggcgatctcg acacttataa
    27301 atgggatggt ctgcgcgatc atacggatat cgctatagat gatgatctct ggcgtcgttt
    27361 gagtaccgac aaagccagct gcctcaaccg taactgttac tactatcgtg aatgcccgtt
    27421 ttttgttact cgtcgggaga ttcaggaagc ggaagtggtg gtggcaaacc atgcgctggt
    27481 gatggcggcg atggaaagcg aagccgtact gcctgacccg aaaaatctgc tgctggtgct
    27541 ggacgaaggt catcacctgc cagatgtggc ccgggatgcg ctggagatga gcgctgaaat
    27601 caccgtgccc tggtatcgac tacagctgga cttgttcacg aaactggtcg ctacctgcat
    27661 ggagcagttt cgcccgaaga ccatcccacc gctggcgatc cctgaacgtt tgaatgcgca
    27721 ttgtgaagag ttgtatgagc ttatcgcctc gttaaacaac attctcaatc tctacatgcc
    27781 tgccgggcag gaggcagagc accgttttgc gatgggcgaa ctgcctgatg aattgctgga
    27841 gatctgccag cggctggcaa aactcaccga gatgctgcgt ggtctggcgg agttatttct
    27901 taacgattta agtgagaaaa ccggcagcca cgatattgtg cgtctgcatc ggttgatttt
    27961 acagatgaac cgcgcgctgg ggatgtttga ggtgcaaagc aaactctggc ggctggcgtc
    28021 tctggcgcaa tcttccggtg cgccggtgac caaatgggcg acgcgggaag agcgcgaagg
    28081 gcagctacac ctctggtttc actgcgtggg aatacgtgtc agcgatcagc tggaaaggct
    28141 gctgtggcgc agtattccgc acattattgt cacctccgca accttgcgtt cgctgaatag
    28201 tttttcgcgt ttgcaggaga tgagtggtct gaaagagaaa gcgggcgacc gttttgtggc
    28261 gctggattcc ccctttaacc actgcgaaca gggcaaaatt gttattcccc ggatgcgctt
    28321 tgagccttcc atcgacaacg aagagcaaca cattgccgaa atggcggcct ttttccgtga
    28381 acaggtggag agcaaaaaat atctcggtat gttggtactg tttgccagcg gacgggcgat
    28441 gcagcgcttt ctcgactatg tgacggattt acgcctgatg ttgctggtgc agggcgatca
    28501 gccgcgttat cgcttagttg aactgcaccg caaacgcgtt gccaacggtg agcgtagcgt
    28561 gctggtgggc ttacagtcat ttgccgaagg gcttgatttg aaaggtgatc tgctcagcca
    28621 ggtgcatatc cacaaaatcg cttttccgcc catcgacagc ccggtggtga tcaccgaagg
    28681 ggaatggctg aaaagcctca accgctatcc gtttgaggtg caaagcctgc cgagcgcctc
    28741 gtttaacctg attcagcagg ttgggcgact gattcgaagc cacggttgct ggggcgaagt
    28801 ggttatctac gacaaacgct tgctgacaaa aaattatggc aagcgactac tggatgcatt
    28861 accggtattt ccgatagagc aaccggaagt ccctgaaggt atagttaaaa agaaagaaaa
    28921 aacgaaatcc ccacgccgtc ggcggcgtta atgatgtgag tcaggtaagg agtcgtaaat
    28981 ggactatcgc aaaatcatta aagagatcgg gcgcgggaaa aaccacgcgc gcgatttaga
    29041 ccgggatact gcgcgcggtc tgtatgctca tatgctcaat ggtgaagtcc ctgaccttga
    29101 gttgggcggc gtattgattg cgctgcgtat caaaggggaa ggggaagcag agatgctcgg
    29161 cttttacgaa gccatgcaaa atcacaccat caagctgacg ccgcctgcag gcaagccgat
    29221 gccgattgtc atccccagtt acaacggcgc gcgtaaacag gctaacctga cgccattatt
    29281 ggcgattctg ctgcataaac tcggttttcc tgtggtggtt cacggagtta gcgaagatcc
    29341 aactcgcgtg ctgacagaaa ccattttcga actgatgggt attacaccaa cgttgcacgg
    29401 cgggcaggcg caggcgaagc tcgacgaaca tcaaccggtg tttatgcctg tcggcgcgtt
    29461 ttgcccgccg ctggaaaaac aactggcgat gcgctggcgg atgggcgtgc gtaacagtgc
    29521 acataccctg gcgaaactgg cgacgccatt tgccgaaggt gaggcactgt gtctttccag
    29581 cgtttcgcat ccggaataca ttggacgcgt cgcgaagttc tttagcgata tcggcgggtg
    29641 ggcgttacta atgcatggta ctgaaggcga agtgtatgct aatccgcagc gctgcccgca
    29701 gatcaatctc attgaccgtg aagggatgcg ggtgctgtat gaaaaacagg accctgctgg
    29761 tagcgagtta ctgccacaag caaaagatcc ggaaaccacg gcgcagtgga ctgagcgttg
    29821 cctggctggc agcgaagcga ttcccgaatc gctgaaaatc cagatggctt gctgcctggt
    29881 ggctacgggt gaagcggcaa ctatcagcga tggcctggcg cgcgttaatc aggcatttta
    29941 atttttatct cagccggatg agccacgtca tccggctttt accccaccgc aatatgaaat
    30001 tcctgcatct ttattgacct tcccgcgccc ggcgtgcagc ataaaaaaac aacaaacaca
    30061 taacataaac aggagttaac catggaaagt ggtcatcgct ttgatgctca gacgctgcac
    30121 agttttattc aggctgtatt tcgtcagatg ggtagcgagg aacaagaagc gaaattagtt
    30181 gccgatcatt taatcgcggc aaacctggca gggcatgatt ctcatggtat tggcatgatc
    30241 ccaagctatg tgcgttcctg gagtcagggg catctgcaaa ttaaccatca tgccaaaatc
    30301 gttaaagagg cgggggcggc agtcacgctc gatggcgatc gcgcattcgg tcaggtcgcg
    30361 gcacacgaag cgatggcgct ggggattgag aaagcgcatc agcacggtat tgctgccgtg
    30421 gcgctacata actcgcatca tatcggccgt atcggttact gggcggagca gtgttcagcg
    30481 gcggggtttg tctctatcca ctttgttagc gtggtcggta ttccaatggt cgcgccgttc
    30541 cacggtcgcg acagccgctt tggcaccaat cccttctgtg tggttttccc tcgtaaagat
    30601 aatttcccgc tgttgcttga ttacgccacc agcgccattg catttggcaa aacccgcgtc
    30661 gcctggcata aaggcgtccc cgtgccgcca ggttgcctga ttgacgttaa cggcgtgccg
    30721 acgaccaatc cggcggtaat gcaggagtcg ccgttggggt cgctgttgac ctttgccgaa
    30781 cataaaggct acgcccttgc agcgatgtgt gaaattcttg gcggggcgct ttctggcggt
    30841 aaaacgacgc atcaggaaac gttgcaaacc agccccgatg ccattcttaa ctgcatgacc
    30901 actatcatca tcaacccgga actgttcggc gcgccggatt gtaacgcgca gaccgaagcc
    30961 tttgccgagt gggtgaaagc ctcgccgcat gatgacgata agccgatttt gctaccgggc
    31021 gagtgggaag tgaacacgcg tcgcgaacgg caggagcagg ggattccatt ggatgcggga
    31081 agctggcagg ccatttgtga tgcggcgcgg cagattggta tgtcggaaga gacgttgcag
    31141 gctttctgtc agcagttagc cagctaaaaa aaagcccgtc cagtggcgga cgggcaaaca
    31201 agggtaacat aggatcaatg agggttagag catatgcgtc tgtcggcaaa cagacaggga
    31261 aatacttgtg ctggacgtag cgtaaacgcc tgatccgtcc aacggttcgg attttgtagg
    31321 cctgataaga cgcggtagcg tcgcatcagg catctgttgc cggatgcggc ataaacgcct
    31381 tatccgtcct acgaattact tataaattac cgcagtaccg cttaatttat tgttgttggt
    31441 ggcggaagtg atgctgtatc cactggcacc ggctgccgcc gctttctcag ccagtttcgc
    31501 ttccagggca tcgagggtgg atgcgccatc ggcagaaacc acgccgattt tattcatgtt
    31561 ctgtgcctgg gatgccgtta ccggttccgc cgcgaacacg ccaaatgaca gagttgaaag
    31621 agccatagca gcaacaacag tattgatagt tttcataatt aatctctcgc aggtgatttt
    31681 tgtataagtg acgttgtttc gtcgatgtga tgagtatcac gtttttttac gagagataaa
    31741 atcgaagaga attgaccgcc ttgttcaaat aaattgattg atatctaaca tactgataaa
    31801 ccatttattt gttgtactta tggacttgtt ttatgccgcg tggcagtcac agcagcgtta
    31861 aaggcacgga atgccacgca aaatacaaat tatactcgct atgcatctcg ccagttgtag
    31921 ttaacgtaac tgtctgtctt tcgaacctct gcgattatat cctgtatacg ctggtttttg
    31981 taaatctttc tcctgctgac aatgaataca taagcgcacg ccaggaatgg cttcccgacg
    32041 ggcttgcggg atgggggcac cgcactcttc acattcatac aggctttcgc cacgcggaat
    32101 ttcaccccga gcgcgggcaa tcgcatcttc aattgtactg ttgatctgtt cgttgacggc
    32161 gtcatcgtta gcccaagcgg atgccatatc gtcctcccca tatcaatgct tgtacagtta
    32221 agtgtagcta atatagggac gaacctgggc agttcaagcg tcagatctcc gaccattccc
    32281 gcagcagatt atgataaaga ttgagcagcg acaggatctc ttcattttca ccatagcggc
    32341 ttttcagcga ctgaatattt ttgtccagtt caaacagcat ggcgcgcttt ttatcatcgc
    32401 ggatcatcga ctggatccac ataaatgatg ccactcgtac gccgcgggtt acgggtgtca
    32461 cgcaatgcag gctgctggag ggatacaaca ccagatcacc tgccgggagt tttacccgat
    32521 gttgtccaaa ggtgtcatta acgaccagtt cgccgccgtc gtagctttgt ggatcgctta
    32581 aaaacagcgt ggcagaaagg tcagtgcgca tccagccgtt ttgtggatgg ctgcgtactg
    32641 cgccatccac atggaaacca taggtttcat tgttctgata gcgattaaac agcggcgtgg
    32701 aaagggtacg cggcaaggca gcggcaaaga acaatgcatg ttggttaacc gcgttcagca
    32761 cctcattttg cagtgcggcg tataacgcgc tgcgggtgtc tacttgctga ttgttcttaa
    32821 cttgtgcacc ttgtgctccg gtggtgacgc gtccatccac ccattcggct tgttccagtt
    32881 gttcgcgaaa atgggcgacg tcctgtggcg ataacacgcc gggaatgtgg tacatcatac
    32941 aagttctcca aaaagtgggg cctgcgcccc acatctgaat cagaaatgca tattggctgt
    33001 gagcaagaag gttcttggct cgcccgggtg ataacggtag ccgctcttgt tgattgaggc
    33061 gacgtaatcg gtatcaaaca ggttgtaaac gtttagctgg aagtcgagat tgcgattaac
    33121 ccgatacccc agtttggcat cggcgaccca gtaaccttct gtaaatgctg gcgttcccac
    33181 cgcgccgtct gaacctttat gtatactgcc gatatagcgt gcgcccgcgc caacagagat
    33241 atcatcggtt gcctgatatt ggctccataa ggtgaaggcg tgctccgggg tatacggcag
    33301 cgatgaggaa ccatcctggg caacatcttt gccgtttttg atggttgctt tttgctgggt
    33361 atagccgcca atcacctgcc acgcgggagt gatattccct gccacggata tctcatagcc
    33421 ttcgacgcgt ttcttaccgt attgcgagta agtcccgtca tcattttgct caacttcatt
    33481 ttcgatatca gtacggaaca gcgcggcggt gagcaacagg cgtttatcca gaacctgcca
    33541 tttggtgcca atctcgctgg tgttggcttt ttgcggttta aaatcggtgc ggttggcact
    33601 gttaccactg ccagactgcg caagggcgaa gttgttgccg cccggaggct gctgggaaac
    33661 ggcatagtta atatagacat tgccgttttc cgtcaggtgg tacagcgccc cggctttcca
    33721 gttcaccaga ttgcccgact tggcggtgtc gacggtggtg actggagaac cttttgccac
    33781 accagctggg caggtgatgg caccgcgtcc gctgccgccg caggcggtgg cactgtcata
    33841 ttcagtatga taattatcca gacggatccc gccgttcagc tcaaaatcac gggtgatttg
    33901 cagcgtatcg aaggcgtaaa ttgcgaaggt atccgtctga ccattggcgt ttgcgccgtt
    33961 gcgcgtcagg ccgccgggat gaattctgct gtcaggatga taaatattta ccgccggtaa
    34021 cgtcaccgga ttaacgccat agttagtctg cgtttcacgg gtaaattcca cgccggtact
    34081 gacatcatga ccgatagaac cggtatagaa cgtcgaggtc aggttggtct ggttggtgag
    34141 aattttatta ctcacatctt tggtattcgc cgtgcgtgac taggtccagc tattcacatc
    34201 gctggtgggc tgagtaatat tcgacgcccc gcccataatc gccgtcatca ggtaatcctg
    34261 ctttacgcgc gaccaacggg tagtattgcg aatggtggtg ttatcgttga tgtcgtgctc
    34321 aaaacgcatg gtggcggtgt cggtggtcga atcgtcgtaa tcggaatccg tgccgtaaaa
    34381 gttatgagta tcaacttttc cggaatgatt cagggccgcc gctcccgcag atggggcaga
    34441 atagcccggc aaaccgatgg tcggaatgcc gccgtctggc gtgttgtgct gagtgacatg
    34501 cagataatta agatacaaac gatttgctgt accaaggcca aaagcgacag aaggggcgac
    34561 gccgtaacgc tcatttttga ctttgtcgcg tccggcatcg tgggtttttt cgcccatcac
    34621 attcaggcgc accgcggtag tatcaccaat gacctgattg acgtccagcg tgccgcggcg
    34681 gaaccaggcg ctgccaatac tggcggaggc gtcaatgccg gaatcattgc gcggctgctt
    34741 gctgatcata ttgatcgagc ctgtcggtgc gctgcgcccg tagtcggtgc cggacggccc
    34801 tttaatcact tcgacctgct cggtattgaa ggtgtcgcgc gagacgctgc cgatatcgcg
    34861 aataccatca atataaatac tgttagaggt atcggcacca cgcatataaa tggcgtcgcc
    34921 agtggtggag ttaccgttct cacccgcaaa aaacgcaccc acgccgg
//
LOCUS       DAJTUI010000034        34731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTUI010000034 DAJTUI010000000
VERSION     DAJTUI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.034"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            859..3144
                     /gene="nrdA"
                     /locus_tag="OO464_001814"
     CDS             859..3144
                     /gene="nrdA"
                     /locus_tag="OO464_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6226984.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3378..4508
                     /gene="nrdB"
                     /locus_tag="OO464_001815"
     CDS             3378..4508
                     /gene="nrdB"
                     /locus_tag="OO464_001815"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6226985.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4508..4762
                     /gene="yfaE"
                     /locus_tag="OO464_001816"
     CDS             4508..4762
                     /gene="yfaE"
                     /locus_tag="OO464_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6226986.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4816..5466)
                     /gene="inaA"
                     /locus_tag="OO464_001817"
     CDS             complement(4816..5466)
                     /gene="inaA"
                     /locus_tag="OO464_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR6226987.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5645..5887
                     /locus_tag="OO464_001818"
                     /pseudo
     CDS             5645..5887
                     /locus_tag="OO464_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5929..7005)
                     /gene="glpQ"
                     /locus_tag="OO464_001819"
     CDS             complement(5929..7005)
                     /gene="glpQ"
                     /locus_tag="OO464_001819"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6226988.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7010..8368)
                     /gene="glpT"
                     /locus_tag="OO464_001820"
     CDS             complement(7010..8368)
                     /gene="glpT"
                     /locus_tag="OO464_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6226989.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8641..10269
                     /gene="glpA"
                     /locus_tag="OO464_001821"
     CDS             8641..10269
                     /gene="glpA"
                     /locus_tag="OO464_001821"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6226990.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10259..11518
                     /gene="glpB"
                     /locus_tag="OO464_001822"
     CDS             10259..11518
                     /gene="glpB"
                     /locus_tag="OO464_001822"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6226991.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11515..12705
                     /gene="glpC"
                     /locus_tag="OO464_001823"
     CDS             11515..12705
                     /gene="glpC"
                     /locus_tag="OO464_001823"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6226992.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12898..13848
                     /locus_tag="OO464_001824"
     CDS             12898..13848
                     /locus_tag="OO464_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6226993.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13889..14359)
                     /locus_tag="OO464_001825"
     CDS             complement(13889..14359)
                     /locus_tag="OO464_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6226994.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14414..15616)
                     /locus_tag="OO464_001826"
     CDS             complement(14414..15616)
                     /locus_tag="OO464_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR6226995.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15703..16245)
                     /locus_tag="OO464_001827"
     CDS             complement(15703..16245)
                     /locus_tag="OO464_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6226996.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16524..16949
                     /gene="nudI"
                     /locus_tag="OO464_001828"
     CDS             16524..16949
                     /gene="nudI"
                     /locus_tag="OO464_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6226997.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16988..17590)
                     /locus_tag="OO464_001829"
     CDS             complement(16988..17590)
                     /locus_tag="OO464_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6226998.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17898..19037
                     /gene="arnB"
                     /locus_tag="OO464_001830"
     CDS             17898..19037
                     /gene="arnB"
                     /locus_tag="OO464_001830"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR6226999.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19041..20009
                     /gene="arnC"
                     /locus_tag="OO464_001831"
     CDS             19041..20009
                     /gene="arnC"
                     /locus_tag="OO464_001831"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6227000.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20009..21990
                     /gene="arnA"
                     /locus_tag="OO464_001832"
                     /pseudo
     CDS             20009..21990
                     /gene="arnA"
                     /locus_tag="OO464_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21987..22877
                     /locus_tag="OO464_001833"
     CDS             21987..22877
                     /locus_tag="OO464_001833"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6227001.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22877..24529
                     /gene="arnT"
                     /locus_tag="OO464_001834"
     CDS             22877..24529
                     /gene="arnT"
                     /locus_tag="OO464_001834"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR6227002.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVTTQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24526..24861
                     /gene="arnE"
                     /locus_tag="OO464_001835"
     CDS             24526..24861
                     /gene="arnE"
                     /locus_tag="OO464_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6227003.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24861..25247
                     /gene="arnF"
                     /locus_tag="OO464_001836"
     CDS             24861..25247
                     /gene="arnF"
                     /locus_tag="OO464_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6227004.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25241..25513)
                     /gene="pmrD"
                     /locus_tag="OO464_001837"
     CDS             complement(25241..25513)
                     /gene="pmrD"
                     /locus_tag="OO464_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6227005.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25618..26973)
                     /gene="menE"
                     /locus_tag="OO464_001838"
     CDS             complement(25618..26973)
                     /gene="menE"
                     /locus_tag="OO464_001838"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6227006.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26970..27932)
                     /gene="menC"
                     /locus_tag="OO464_001839"
     CDS             complement(26970..27932)
                     /gene="menC"
                     /locus_tag="OO464_001839"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6227007.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27932..28789)
                     /gene="menB"
                     /locus_tag="OO464_001840"
     CDS             complement(27932..28789)
                     /gene="menB"
                     /locus_tag="OO464_001840"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6227008.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28804..29562)
                     /gene="menH"
                     /locus_tag="OO464_001841"
     CDS             complement(28804..29562)
                     /gene="menH"
                     /locus_tag="OO464_001841"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6227009.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29559..31229)
                     /gene="menD"
                     /locus_tag="OO464_001842"
     CDS             complement(29559..31229)
                     /gene="menD"
                     /locus_tag="OO464_001842"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6227010.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31318..32613)
                     /gene="menF"
                     /locus_tag="OO464_001843"
     CDS             complement(31318..32613)
                     /gene="menF"
                     /locus_tag="OO464_001843"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6227011.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32692..32997)
                     /gene="elaB"
                     /locus_tag="OO464_001844"
     CDS             complement(32692..32997)
                     /gene="elaB"
                     /locus_tag="OO464_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6227012.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33052..33513)
                     /locus_tag="OO464_001845"
     CDS             complement(33052..33513)
                     /locus_tag="OO464_001845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6227013.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33578..34495
                     /gene="rbn"
                     /locus_tag="OO464_001846"
     CDS             33578..34495
                     /gene="rbn"
                     /locus_tag="OO464_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR6227014.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         8479 a         9317 c         8609 g         8326 t
ORIGIN      
        1 gcgtaatgcc tgaaaggctt tgcctgctgg cgtgacaact cgctttgata tcatatccct
       61 ggcataaatc aatggccgcc gcgacaccgt tagcagagaa aagagatgca atcattgacg
      121 ggagtagaga taaatactcc ttgcgtagaa agataatccg catattaatc tgaccatcat
      181 tttatataag acggcatatt taaacgcatt atacaatatg ggttatcttt cttttgtaaa
      241 ttgtccaacc cgatgcaatg gctattttgt tattatttga tgaataatat cagtgcgtca
      301 taattcaagt taataacctt aagggatacc agttatattt aaactaaatt aaagtaatga
      361 ataattttct tataatataa ggtaaattaa caaaatggct tcgcatttaa caataaccga
      421 atagaaaaca accatttcga catcaacaat cgcttacatt cgcttatata ttgaccacaa
      481 ctgatacatc agattatgtg atgactcgtg cttagatcaa tttttgcaat cattagcaaa
      541 aagattaata aaccatctat atcaatttat ccaacctatt ctgccgttca agaaatcgcc
      601 gaacagttat ttttaacaaa tttttctctt cccattgact ttcccggaca ccttgtctga
      661 cctaaggtgc gcgaaagcca ctttttcctt cctgagttat ccacaaagtt atgcacttgc
      721 aagagggtca ttttcacact atcttgcagt gaatcccaaa cataccccct atatatagtg
      781 ttctaagcag cttcccttac tacaggtagt ctgcatgaaa ctattgcgga aagaattcca
      841 aaaacaggta cgacatacat gaatcagaat ctgctggtga caaagcgcga cggtagcaca
      901 gagcgcatca atctcgacaa aatccatcgc gttctggatt gggcggcaga aggactgcat
      961 aacgtttcga tttcccaggt cgagctgcgc tcccacattc agttttatga cggtatcaag
     1021 acctctgaca tccacgaaac tattatcaag gctgccgcag acctgatctc ccgtgatgcg
     1081 ccggattatc agtatctcgc cgcgcgcctg gcgatcttcc acctgcgtaa aaaagcctac
     1141 ggccagtttg agccgcctgc gctgtacgac cacgtggtga aaatggtcga gatgggcaaa
     1201 tacgataatc atctgctgga agactacacg gaagaagagt tcaagcagat ggacacgttt
     1261 atcgatcacg accgtgatat gaccttctct tatgctgccg ttaagcagct ggaaggcaaa
     1321 tatctagtac agaaccgcgt gaccggcgaa atctacgaaa gcgcccagtt cctttatatt
     1381 cttgttgccg cgtgcctgtt ctcgaactac ccgcgtgaaa cgcgcctgca atacgtgaag
     1441 cgtttttatg acgcggtttc cacatttaaa atttcgctgc cgacgccaat catgtccggc
     1501 gtgcgtaccc cgactcgtca gttcagctcc tgcgtactga tcgagtgcgg tgacagcctg
     1561 gattccatca acgccacctc cagcgcgatt gtgaaatatg tttcccagcg tgccgggatc
     1621 ggcatcaacg ccggacgtat tcgtgcgctg ggcagcccga ttcgcggtgg cgaagcgttc
     1681 cataccggct gtatcccgtt ctacaaacat ttccagacag cggtgaaatc ctgctctcag
     1741 ggcggtgtgc gcggcggtgc ggcaacgctg ttctacccga tgtggcatct ggaagtggaa
     1801 agcctgctgg tgttgaaaaa caaccgtggg gttgaaggca accgcgtgcg tcatatggac
     1861 tacggggtac aaatcaacaa actgatgtat acccgcctgc tgaaaggtga agatatcacc
     1921 ctgttcagcc cgtccgacgt accggggctg tacgacgcgt tcttcgccga tcaggaagag
     1981 tttgaacgcc tgtataccaa atatgagaaa gacgacagca tccgcaagca gcgtgtgaaa
     2041 gccgttgagc tgttctcgct gatgatgcag gaacgtgcgt ctaccggtcg tatctatatt
     2101 cagaacgttg accactgtaa tacccatagt ccgtttgatc cgaccatcgc gccagttcgc
     2161 cagtctaacc tgtgcctaga aattgccctg ccgaccaaac cgctgaacga cgtcaacgac
     2221 gagaacggtg aaatcgcgct gtgtacgctg tctgctttca acctgggcgc aattaatagc
     2281 ctggatgaac tggaagagct ggcgattctg gcggttcgtg cacttgacgc gctactggat
     2341 tatcaggatt acccgatccc ggccgccaaa cgtggagcga tggggcgtcg tacgctgggt
     2401 attggtgtga ttaacttcgc ttactatctg gcgaagcacg gtaaacgcta ctccgacggc
     2461 agcgccaaca acctgacgca taaaaccttc gaagcgattc agtattacct gctgaaagcg
     2521 tctaatgagc tggctaaaga gcaaggcgcg tgcccgtggt ttaacgaaac cacttacgcg
     2581 aaagggatcc tgccgatcga tacctataag aaagatctgg atgccatcgc taatgagccg
     2641 ctgcattacg actgggaagc attgcgtgaa tcaatcaaaa cgcacggcct gcgtaactcc
     2701 acgctttctg ccctgatgcc gtccgagacc tcttcgcaga tctctaacgc cactaacggt
     2761 attgaaccgc cgcgcggtta cgtcagcatc aaagcgtcga aagacggtat tttgcgccag
     2821 gtggtgccgg actacgagca cctgcacgac gcctatgagc tgctgtggga aatgccgggt
     2881 aacgatggct acctgcaact ggtaggtatc atgcagaaat ttatcgatca gtcgatctct
     2941 gctaacacca actacgatcc gtcacgtttc ccgtcaggaa aagtgccgat gcagcagttg
     3001 ctgaaagacc tgctcaccgc ctacaaattc ggggtcaaaa cgctgtatta tcagaacacc
     3061 cgtgacggcg ctgaagacgc gcaggacgat ctggtaccgt caatccagga cgatggctgc
     3121 gaaagcggcg catgtaagat ctgatattga gatgccggat gcggcgtaaa cgccttatcc
     3181 ggcctacggc tcggtttgta ggcctgataa gacgcgccag cgtcgcatca ggctccgggt
     3241 gccggatgca gcgtgaacgc cttatccggc ctacggctcg gatttgtagg cctgataaga
     3301 cgcgccagcg tcgcatcagg cacaggatgc ggcgtaaaat gccttatccg gcattaaact
     3361 cccaacagga cacactcatg gcatatacca ccttttcaca gacgaaaaat gatcagctca
     3421 aagaaccgat gttctttggt cagccggtca acgtggctcg ctacgatcag caaaaatatg
     3481 acatcttcga aaagctgatc gaaaagcagc tctctttctt ctggcgtccg gaagaagttg
     3541 acgtctcccg cgaccgtata gattaccagg cgctgccgga gcacgaaaaa cacatcttta
     3601 tcagcaacct gaaatatcag acgctgctgg attccattca gggtcgtagc ccgaacgtgg
     3661 cgctattgcc gcttatttct attccggaac tggaaacctg ggtcgaaacc tgggcgttct
     3721 cagaaacgat tcattcccgt tcctatactc atatcattcg taatatcgtt aacgatccgt
     3781 ctgttgtgtt tgacgatatc gtcaccaacg agcagatcca gaaacgtgcg gaagggatct
     3841 ccagctatta cgatgaactg atcgaaatga ccagctactg gcaccttctg ggcgaaggca
     3901 cccacaccgt taacggtaaa accgtaaccg ttagcctgcg cgagctgaag aaaaaactgt
     3961 atctctgcct gatgagcgtt aacgcgctgg aagcgatccg cttctacgtc agctttgctt
     4021 gttcctttgc ctttgcagaa cgcgaactga tggaaggtaa cgccaagatt attcgcctga
     4081 ttgcccgcga tgaagccctg cacctgaccg gtactcagca tatgctgaat ctgctgcgca
     4141 gcggcgcgga tgatccggag atggcggaaa ttgccgaaga atgtaagcag gagtgctatg
     4201 acctgtttgt ccaggcggca cagcaagaga aagactgggc ggattatctg ttccgtgacg
     4261 gttcgatgat tggtctgaat aaagatattc tctgccagta cgttgaatac atcaccaata
     4321 tccgcatgca ggcagtcggt ttggatctgc cgttccagac gcgctccaac ccgatcccgt
     4381 ggatcaacac ctggctggtg tctgataacg tgcaggttgc tccgcaggaa gtggaagtca
     4441 gttcttatct ggtcgggcag attgacgcgg aagtggacac cgacgatttg agtaacttcc
     4501 agctctgatg gcccgcgtta ccctgcgcat cactggcaca caactgctgt gccaggatga
     4561 acacccttcc cttctggcgg cgctggaatc ccacaatgtg gcggttgagt accagtgccg
     4621 cgaaggttac tgcggctcct gtcgcacccg actggtcgca ggtcaggtgg actggattgc
     4681 cgaaccgtta gcctttattc agccggggga aattttgccc tgttgttgcc gggcaaaagg
     4741 cgatattgaa atcgagttgt gatcgggtac tagcgccaga tacgacgctt acgcgtatta
     4801 tctggcctac aacgattaca tggcgtagta atacgctttc acctgctccc agtccgcttt
     4861 agggatcggc tccagatatt tttccagctg gcggaagtca tgattaatcg ctttatcgcg
     4921 acgcaagcgg cgacgacttt tctccagatc aagaaaacca gcttctgcct taccttctgt
     4981 tttcacatag atatggcgaa catagcaaca gccatgctga cgattaacgc tatgcatttt
     5041 cttaaacgcc agtgccactg ctttcaacat ggcttgccgt acttcgtcag aataaggcga
     5101 tactgcatgc cgggcatacc agtcagcaat gctgatgaac cccgccatat cttcagtcac
     5161 cagcaacgcg cgccattcac cctcaatttt caccgcttca ccaaaaacga tcttcggcac
     5221 gatgacaccg gcccgttcca gttctttaat taccgcaacc tcacggacaa tcgttggtcg
     5281 gccgaacgga taacgtactg aatgaaacag atgatgcgtc atgcgcttta catacagctt
     5341 tttgccgttg cgctcgacgc attgcacccc gctcatacca ttacggcgat agttaggctc
     5401 ttcaacccag tcgccctctg ttgcccacca gtgattaaat tcgtcgtact ttgctgaaac
     5461 tgccataccc tatcgcctgt catttttatt aacgacaatg actataggtg gttacctgag
     5521 gaaaatctta atgaaacgtg tcgtattaat gaatttcttt tgccgctaca ggtgtgacac
     5581 cgcttttgcc cttcatttgc agcgcataga ttccaccagc cactaccact aatagtgcgc
     5641 ccagcagata aaatgtgaat ttaatcagtc ggatacagcg atgaatttta ttcgacaggg
     5701 attaggcatc gccttacaac cagagttaac gctgaaaagc attgcaggtg aattgtgttc
     5761 cgttcctcac gaaccaacct tctatcgaca gatttcgttg ctgactaaag aaaagccggt
     5821 agaaggcagt ccactgtttt tactacaaat gtgcatggaa caattagtgg cgattggaaa
     5881 aatttgatat gagacaggat ggcgcatgaa cgccatcctg cagtaacatt actctttatt
     5941 aagaaatttt actgccttat cagggaaatc agtaaacagc ccatttacac ccgctttgtt
     6001 atacagagca tcatataact gattcacatc aggagtgtat tcaggcagtt tatctgaccg
     6061 cacggtataa ggatgcacta ccagtttatt ctgctgggca tcttgcacca tgccagtgag
     6121 tttgatatta cccggctgcg atgtctcctc aatcaacata tggtaatccg gaccaatacc
     6181 atctgcatat tccgccacct gtttcatggc tcccggctta aacatccagt cgtagttgta
     6241 attaacccag cttccatccg gctgtttctg ctgcgtttca ttccagtcgg tataggcaat
     6301 cagctgtacc agattgagct ccatgcccat tttgggttcc agctcattct taatacgctt
     6361 cagctcatca gcatcaaaac attgcaaata aactttatcg tctttaccgg tgtaaccata
     6421 tttcttcagc acttccagcg tttttgccgc aatatccttc ccttcctgat gatggaacca
     6481 cggcgctttg atttctggat agataccgat atttttcccg gtagagtgat ttaacccctg
     6541 aacaaattca atctcttctt caaaggtgtg cacccggaag tcggacttac ccattgggaa
     6601 acgccccgga taagtctgta cttttttacc gttttcaata tcgaaacctt cggtaaattt
     6661 cagcgactta atttcatcca gcgtgaaatc tatcgcgtag taacgaccgt ctttgcgcgc
     6721 ccgatccggg aaacgatcgg caacatcagt aacacgatcg agataatggt catgcagaac
     6781 aaccagatgg tcgtctttgg tcatcaccaa atcctgttcc agataatccg ctccctgcgc
     6841 atacgccatc gcttttgctg gcagcgtatg ctccggcaaa tatccactgg caccgcgatg
     6901 ggcgatgact attttttcgt tgctgtccgc cgccattgca ctgcttccca tgactatagt
     6961 gctcatcatg atcgccatgc taaggttttt cagcttcaat ttcatgccat tagcctccgt
     7021 tgcgtttttg cagtaattgt tcatggcgac gtttttcgcc aatcatcaca acaatcaaca
     7081 agataaccgc cagaatgctg ccgccaatca ttaccataaa gccgccatcc cagccgaaga
     7141 agtccacggt gtagccaaca atcgcgctcg ccgccaccga accgcccagg taaccaaaca
     7201 gcccggtaaa gcccgctgcc gtacctgccg cttttttcgg tgccagttcc agcgcatgca
     7261 gaccgatcag catcacagga ccgtagatca ggaagccgat aacaatcata caaatcatat
     7321 cgacggttgg gttacctgcc gggttcatcc agtaaacgat agtcgcgatg gtcaccagtg
     7381 tcataaagaa gacgccggtt gccccacggt taccacggaa gactttgtcc gacatccagc
     7441 cgcacagcag ggtgcccgga atacccgcat actcatagaa gaagtaggcc caggaggatt
     7501 tatccagcgc gaaatgctta acctctttca gataagtcgg tgaccagtcg aggatgccgt
     7561 aacgcagcag ataaacgaac acgttggcga tggcgatgta ccacagcagt ttgttcggca
     7621 gcacgtactg catgaagatt tgcttcgcgg tcagctcctg ttccgctttt tcgttatagt
     7681 cgtccggata atcatttttg tactcttcga tcggcggcaa gccacaggat tgcggggtat
     7741 cgcgcatcat cgcaaaggcg aataatgcca ccagaatggc gcagaaagca ggcatataga
     7801 gcgccgcatg ccagtcattg aaccaggcca tccccagcag gaacagcagc ggcggaatac
     7861 caccaccgac gttgtgcgca cagttccaca ctgacacaat gccgccacgt tctttctgcg
     7921 accaccagtg caccatagta cgaccacacg gcggccaccc catcccctgg aaccaaccgc
     7981 agaggaacaa cagtacaaac atcaccgcaa tgctcgacgt cgcccatggc acaaagccca
     8041 taaacaacat cactgccgcc gccagaatca aacctgcggg caggaaaacg cgcggattcg
     8101 agcgatccga taccgaaccc atgatgaatt tcgaaaatcc ataagcaatc gagatccccg
     8161 aaagggcaaa acctaaatca ccgcgtgaga atccctgctc aaccagataa ggcatagcaa
     8221 gcgcaaagtt cttacgaacc aaatagtaag ccgcatagcc aaagaatatc cccaggaaaa
     8281 tttgccagcg caatcgacga taagtcggat cgatctccgc ggcaggtaag cgcgctttgt
     8341 gtggcgctgg tttaaaaata ctcaacattg atagcctccg tggcccgtgg tcttatttat
     8401 gattaacagc ctgattcagt gagagaacct gccgtttctt gagttgccgc gatgttaaga
     8461 aaacattcat aaattaaatg tgaattgccg cacacattat taaataagat ttacaaaatg
     8521 ttcaaaatga cgcatgaaat cacgttttac tttcgaatca tgagcgaata tgcgcgaaat
     8581 caaacaattc atgtttttac tatggctaaa tggtaaaaaa cgaacttcag agggataaca
     8641 atgaaaactc gcgactcgca atcaagtgac gtgattatca ttggcggcgg cgcaacggga
     8701 gccgggattg cccgcgactg tgccctgcgc gggctgcgcg tgattttggt tgagcgccac
     8761 gacatcgcaa ccggtgccac cgggcgtaac cacggcctgc tgcacagcgg tgcgcgctat
     8821 gcagtaaccg atgcggaatc ggcccgcgaa tgcattagtg aaaaccagat cctgaaacgc
     8881 attgcacgtc actgcgttga accaaccaac ggcctgttta tcaccctgcc ggaagatgac
     8941 ctctcattcc aggccacttt tattcgcgcc tgcgaagaag cagggatcag cgcagaagct
     9001 atagacccgc agcaagcgcg cattatcgaa cctgccgtta acccggcact gattggcgcg
     9061 gtgaaagttc cggatggcac cgttgatcca tttcgtctga ccgcagcaaa catgctggat
     9121 gccaaagaac acggtgccgt tatccttacc gctcatgaag tcacggggct gattcgtgaa
     9181 ggcgcgacgg tgtgcggtgt tcgtgtacgt aaccatctca ccggcgaaac tcaggccctt
     9241 catgcacctg tcgtggttaa tgccgctggg atctgggggc aacacattgc cgaatatgcc
     9301 gatctgcgca ttcgcatgtt cccggcgaaa ggatcgctgc tgatcatgga tcaccgcatt
     9361 aaccagcatg tgatcaaccg ctgccgtaaa ccttccgacg ccgatattct ggtgcctggc
     9421 gataccattt cgctgattgg taccacctct ttacgtattg attacaacga gattgacgat
     9481 aatcgagtga cggcagaaga ggttgatatt ctgctgcgtg aaggggaaaa actggccccc
     9541 gtgatggcga aaacgcgcat tttgcgggcc tattctggcg tgcgcccgct ggttgccagc
     9601 gatgacgacc cgagcggacg taacgtcagc cgtggcatcg tgctgctcga ccatgctgaa
     9661 cgcgatggtc tggacggatt tatcaccatc accggtggca aactgatgac ctatcggctg
     9721 atggctgaat gggctaccga cgcggtatgc cgcaaactgg gcaacacgcg cccctgtacg
     9781 actgccgatc tggcactgcc tggttcacaa gaacccgctg aagttacctt gcgtaaagtc
     9841 atctccctgc ctgccccgct gcgcggttct gcggtttatc gtcatggcga tcgcacgcct
     9901 gcctggctga gcgaaggccg tctacaccgt agcctggtat gtgagtgcga agcggtaact
     9961 gcgggtgaag tgcagtacgc ggtagaaaat ttaaacgtta atagcctgct ggatttacgc
    10021 cgtcgtaccc gtgtggggat gggcacctgc cagggcgaac tctgcgcctg ccgcgctgcc
    10081 ggactgctgc aacggtttaa cgtcacgacg tccgcgcaat ctatcgagca actttccacc
    10141 ttccttaacg aacgctggaa aggcgtgcaa cccatcgcct ggggagacgc actgcgcgaa
    10201 agcgaattta cccgctgggt ttatcaggga ctgtgtggtc tggagaagga gcagaaagat
    10261 gcgctttgat actgtcatta tgggcggcgg cctcgccgga ttactctgtg gcctgcaact
    10321 gcaaaaacac ggcctgcgct gtgccattgt cacgcgtggt caaagcgcgt tgcatttctc
    10381 atccggttcg ctggatttgc tgagccatct gccagatggt caacccgtgg cagacattca
    10441 cagtggactg gaatctttgc gtcagcaggc acctgcacat ccttactctc ttctcgggcc
    10501 acaacgcgtg ctcgatctcg cttgccaggc gcaggcatta atcgctgaaa gcggtgcgca
    10561 attgcagggc agcgtagaac tggctcacca gcggattacg ccgctcggca ctttgcgtgc
    10621 aacctggtta agttcgccag aagttcccgt ctggccgcta cccgcgaaga agatatgtgt
    10681 agtggggata agcgggctga tggatttcca ggcgcatctt gcggcggctt cgttgcgcga
    10741 actggatctt tcggttgaaa ccgcagaaat agagctgccg gaactggatg tcctgcgcaa
    10801 taacgccacc gaatttcgcg cggtgaatat cgcccgtttc cttgataatg aagaaaactg
    10861 gccgctgtta cttgatgcgc ttattcctgt cgccaatacc tgcgaaatga tcctgatgcc
    10921 cgcctgcttc ggcctggccg atgacaaact gtggcgttgg ttgaatgaaa aactaccttg
    10981 ttcactgatg cttttgccaa cgctgccgcc ttccgtgctg ggcattcgtc tgcaaaacca
    11041 gttacagcgc cagtttgtgc gccaaggtgg agtgtggatg ccgggcgatg aagtgaaaaa
    11101 agtgacctgt aaaaatggcg tagtgaatga aatctggacc cgcaatcacg ccgatattcc
    11161 gctacgtcca cgtttcgcgg ttctcgccag cggcagtttc tttagtggcg gactagtagc
    11221 ggaacgtaac ggcattcgcg agccgattct cggccttgat gtgctacaaa ccgccacgcg
    11281 gggtgaatgg tataagggcg atttttttgc gccgcaaccg tggcagcagt tcggtgtaac
    11341 cactgatgag acgctacgcc cgtcgcaggc agggcaaacc attgagaacc tgtttgccat
    11401 cggttcggtg ctgggcggat ttgatcccat cgcccaggga tgcggcggcg gtgtttgtgc
    11461 cgtcagtgct ttacatgccg ctcaacagat tgcccaacgc gcaggaggcc aacaatgaac
    11521 gacaccagct tcgaaaactg cattaagtgc accgtctgca ccaccgcctg cccggtgagc
    11581 cgggtgaatc ccggttatcc agggccaaaa caagccgggc cggatggcga gcgtctgcgt
    11641 ttgaaagatg gcgcactgta tgacgaggcg ctgaaatatt gcatcaactg caaacgttgt
    11701 gaagtcgcct gcccgtccga tgtgaagatt ggcgatatta tccagcgcgc gcgggcgaaa
    11761 tatgacacca cgcgcccgtc gctgcgtaat tttgtgttga gtcataccga cctgatgggt
    11821 agcgtttcca cgccgttcgc accaatcgtc aacaccgcta cctcgctgaa accggtgcgg
    11881 cagctgcttg atgcggcgtt aaaaatcgat catcgtcgca cgctaccgaa atactccttc
    11941 ggcacgttcc gtcgctggta tcgcagcata gcggctcagc aagcacaata taaagaccag
    12001 gtcgctttct ttcacggctg cttcgttaac tacaaccatc cgcagttagg taaagattta
    12061 attaaagtgc tcaacgcaat gggtaccggt gtacaactgc tcagcaaaga aaaatgctgc
    12121 ggcgtaccgc taatcgccaa cggctttacc gataaagcac gcaaacaggc aattacgaat
    12181 gtagagtcga tccgcgaagc tgtgggagta aaaggcattc cggtgattgc cacctcctca
    12241 acctgtacat ttgccctgcg cgacgaatac ccggaagtgc tgaatgtcga caacaaaggc
    12301 ttgcgcgatc atatcgaact ggcaacccgc tggctgtggc gcaagctgga cgaaggcaaa
    12361 acgttaccgc tgaaaccgct gccgctgaaa gtggtttatc acactccgtg ccatatggaa
    12421 aaaatgggct ggacgctcta caccctggag ctgttgcgta acatcccggg gcttgagtta
    12481 acggtgctgg attcccagtg ctgcggtatt gcgggtactt acggtttcaa aaaagagaac
    12541 taccccacct cacaagccat cggcgcacca ctgttccgcc agatagaaga aagcggcgca
    12601 gatctggtgg tcaccgactg cgaaacctgt aaatggcaga tagagatgtc cacaagtctt
    12661 cgctgcgaac atccgattac gctactggcc caggcgctgg cttaaactcc cttctgatgc
    12721 ccggtaagca tgtggttacc gggcattttt gcgtacacga ttccgtgccc aatgtatgcg
    12781 ttgcaacgca gtgaaaattc ctctgaaaac gtctcgcaaa ggctgaaact ggcagatgtc
    12841 aaaggcctgg gataaccgta atgtcgcgtc atcataaata tcaggtgacg gacaaccatg
    12901 accgaatcaa caacctcctc cccgcatgat gcggtattta aaacctttat attcacaccc
    12961 gaaaccgcac gggattttct cgaaatacat ttaccagaac cactgcgcaa gctttgcaac
    13021 ctgcaaacct tacgcctgga acccactagt tttattgaaa aaagtttacg cgcttactac
    13081 tcggatgttt tgtggtccgt ggaaaccagc gacggtgacg gctatatcta ctgcgtgatt
    13141 gaacatcaaa gctctgcaga aaagaatatg gcttttcggc caatgcgcta tgccactgcc
    13201 gccatgcaga gtcacctaga taaaggctat gacagagttc cgctggtggt gccattgctg
    13261 ttttatcatg gcgaaacatc gccctacccg tactcactta actggctgga tgagtttgac
    13321 gatccgcaac ttgcccggca gttgtacacc gaagcttttc cgttggtgga tatcaccatc
    13381 gtacctgacg atgagatcat gcaacatcgg cgtatagctc tgctggaact gattcaaaag
    13441 catattcgcg accacgattt aatcggcatg gtcgacagga tcaccacgct tttggttaga
    13501 ggcttcacta atgacagcca gctacaaaca ctgtttaatt atctgctgca atgcggcgat
    13561 acctcccgtt tcacccgttt tattcaggag attgccgaac gttcaccact acaaaaggag
    13621 agattaatga ctattgctga acggctacgg caggaaggac atcaaattgg ctggcaagaa
    13681 ggtaaattag taggtttgca gcaaggtaaa ttagaaggtt tgcaggaagg tatgcatgaa
    13741 caagccatta aaattgcttt gcgcatgctg gaacagggca ttgatcgtga ccaagtgctc
    13801 gcggccaccc agctaagcga agccgatctg gcagcgaata accactaatt aacacaggcc
    13861 cacagccgat ccccatgggc ctttgatatc aataactacc ttttatgcgt ggcccatttt
    13921 tgcctgattt aaacatcgcc agtcgttgat ccagggcatc gctgtagagc atcgtgtcaa
    13981 cgccaacagc gacaaagttc gctccccacg ccaggcattg ctgcgccata tcaggagcca
    14041 cagccagaaa accagccgct ttacccgcag cacggatccg ccgaatgctg gtttcaataa
    14101 ttcgctgcac ttccgggtgc ccggcgttat ccgggtagcc caacgacgca gaaagatccg
    14161 ctggtccaat aaacacgcca tcaatccctt cgacgtcgag gatctcgtcc aggttatcca
    14221 gtgccgtttt actttccacc tgcactaaca gacaaagcga atcgttgact tgcgccatgt
    14281 aattctcaat gcgtccccag cgcgcagccc gtgccacact ggccccaaca ccacgctcac
    14341 cgtagggagg gttggacaaa ttttgccgga gggggaatgt gccggtgcag taaaccggca
    14401 cagagtggcg aaatcagacg gataaagtca tggactcaac aacctcaatc cagccatgct
    14461 cactagcgat gtcctggccg tttaaccaac ggcgtagcat attcagtgcc atcattgcgc
    14521 acacttcctg acggatagcc aggctgtagc gtgtagtgct gaaacgcaca cgcagagcga
    14581 aagtgccgtc tggcgtggct agcgcaaagt tgagatgctc gttctcgaaa cccgaaacag
    14641 ccagtgccag cccggcaaaa tggttggccc gccgttctgt aatccagtgc gcagtttgcg
    14701 ccagggtttc ttcctgtgaa ggaatcactt cacacgccag caatggagca cctgcgcgag
    14761 aaagttgcaa agccaataaa ccaccggtga attgctcgct caacgtcagg ctgaactggc
    14821 ggttttgcaa ttcgcgactg atctgcgcgg gcagtccttc ggtgccttcg aaaatcacgc
    14881 tctgtccggc aacacgttta acgtccagcc acagtttttc catcgcctgt tgctcgcttg
    14941 ccggtccggt gagtttcagt tcgatgatcg gcattgagga gcgatagccc attgttacgc
    15001 ccggcggcag ttgtagagtg tccaggcttt gtgccagatc gctttccgaa cgaccaaagg
    15061 tagttaaacg cagacaaacc ggcggctgtg gtaaagaaaa acgctcgcgc aggcgcggca
    15121 ggatttcgtg ctcgaccatc accttaaatt ctgacggtac gccgggagta aagaacatca
    15181 ggcaacgatt aagctgcacg gcaaaaccac aggcggtgcc taccgggtta ttgataaatt
    15241 cagcactggc aggcagctcc gcttgtttac ggttgctcgg tgccattact cgtccacgtt
    15301 cgtggaaata gcgttccatc tctttgagcc aggcttcatg cagcaccagg ccttcacctt
    15361 ttgctgtcgc agcggcgagt gcgcttaaat catcgctggt cggtcccagc ccgccgttaa
    15421 cgatcagcac atcggcgtgc tgactacgtt cgcgaagaat ggtgactaag tcatcaaggt
    15481 tatcccccac cgtattgcgg cgagataatg gcagcccctg atgaaagaaa aaatcggcca
    15541 gccaggcagc gttagtgtca acgatttgcc cgtataacac ttcatccccg gtggatagca
    15601 tttccacttt taacatggtt atctcctgct tttgttatcg aatcactata acgcaaaggg
    15661 agataaggcg tagtaagaaa ctgaatgccg cgccggaagg gatcagaaac tggcgttaac
    15721 cccaacatac aggccgtcag ccacagcgtt gtcgcggtta ccgtctttac ccgacagatt
    15781 caggtagcga taacccgcct caatactgac tggacgcata atggtgtaac gcgcgccagc
    15841 attcgcttcc tcataacttt gaataccgct ggagagcgaa tccggagagt agtaatactc
    15901 gccaaacaaa cggaagctgt tgccaatttt ccactgcaaa ccacctccta ccgctgcggc
    15961 ataaccttca tcgccgtaat tcgggttggt gtacacgcct tttccgccaa cggtcgccat
    16021 taacggcccg agaggcagat tcaaccccag ccccacgccc gcgacgtcac cgtcgtcgtc
    16081 gttatgtgtc cagttaccgc ttaaagccag gcccgtcgat tcagtcccaa aaccgacacc
    16141 aatattggtg tattctttac ccgcctggcc gctgatgctc attgcattga ccgatgcaga
    16201 aaccaacagc attccggcca gacctgctag cgcaattttt ttcattatcc cctttcccac
    16261 aagcaattaa tcaaaattcc caaagctgcg ggattgtact gcctggcaca gaggattcaa
    16321 tgagcgaaaa aagggatcta gtcacttatt gtaactaaat acttcttaaa taattaaacg
    16381 ccgaaaaaat aaacacactt taccgataca cacaaaaata ataataagtg cagttatcat
    16441 tgcaaaagag attactgtca ctttcacata tttctgagtt accgtattct catggtttct
    16501 ttttatttat aaggaatacc cctgtgcgac aacggactat tgtatgccct ttgattcaaa
    16561 atgatggtgc ttatttgctg tgcaaaatgg ccgacgatcg cggcgttttc cccggtcaat
    16621 gggcgctttc gggtggcggc gtggagtctg gcgaacgaat tgaagaggca ctacgccgcg
    16681 aaattcgcga agaactggga gaacagctgc ttttgacaga aatcacgccg tggaccttca
    16741 gcgatgatat tcgcaccaag acatatgcag atggtcgcaa ggaagagatt tatatgattt
    16801 acctgatttt tgactgcgtt tctgccaacc gagaagtgaa aataaacgaa gagtttcagg
    16861 actacgcgtg ggtaaaacct gaagatctgg tgcattatga tttaaatgtt gccacccgaa
    16921 aaacgttacg tttgaaaggt cttctttaac aatgatgaca gccatcactg gctgtcagat
    16981 tgaaaattta gtggttaacg aattccccat ccagataaac tttgcctttt tccacatgca
    17041 tgactaaacc atccagataa tcaggtttaa atatcgcatc acgcttattt ttagcaatat
    17101 atgtcaggca atgattatgg gtgaaaataa cgatattttt atcaggcgct ttgctttgta
    17161 agtccttaat tgcactataa atctcattac cgcactgaag aagtcgttta tctaccgtca
    17221 gctttttacc cgcggaaaac caggtcgccg actgaatggt ccggacggta ttactggaat
    17281 aaagatcgaa atcagagatg tcagcgctaa aagcgttgcc cagttcacgg gcatcctggg
    17341 tacctttaac cgtaatacct gttttatctg acaagcattg attggttgaa cgatcgcaac
    17401 gttcagcatg gcgaaacaaa acgacaaccg ggtgctgctg tgccagtctg gccaatgttt
    17461 tattgtcgat acgtggcaaa ccattgctac tccaggcggc atgagtaccc agtccggcaa
    17521 ttgcagcgag cgccagtaaa atgatgaaat atttttttga cttcaatgat gagcggcaaa
    17581 aagctaacac gtaaactcca cctatagaca agcgcaacca gacaattacc gtgaaattga
    17641 gcgacatttc tggcgataat tagcagttgg tgtaatatta aaaatcctac gatgtcggca
    17701 aaatgcctca aatttttgcc aaatgcaaag cctaaataag aaaaaatata aaaatttcaa
    17761 tatttacgtc taatattagt ttcttaaggt taagttaata ttctatcctt aaaatttcgc
    17821 tccaaatggc aaaatataca caacactctt tatagcaaat ataagtggac aggtattcaa
    17881 tggcggaagg aaaagcaatg tcagaatttt tgcctttttc gcgaccagca atgggcgtgg
    17941 aggaactcgc tgcagttaaa gaggttctcg aatccggttg gatcacaacc ggtccgaaga
    18001 atcaggcgct tgagcaagcc ttttgccagt tgacgggaaa tcagcatgcc atcgcggtca
    18061 gttcagccac cgccggaatg catatcacgc taatggcgtt ggaaattggc aagggcgatg
    18121 aagtgattac gccttccctg acctgggttt caaccctcaa tatgatttcc ttgctgggtg
    18181 caacgccggt aatggtggat gtcgaccgcg atacgctgat ggtcacgcct gaagcgatcg
    18241 aatcagccat tacgccacgc actaaagcca tcattccggt gcattatgcc ggtgcgccag
    18301 cagatattga cgccattcgc gccattggcg aacgttacgg catcgcagtt atcgaagatg
    18361 ctgcccatgc cgtcggtacg tattacaaag ggcgacatat tggcgcaaaa ggtaccgcta
    18421 ttttttcatt tcatgccatt aaaaatatta cctgtgctga aggtggcctg attgtaactg
    18481 ataatgaaaa ccttgcccgc cagctacgga tgctgaaatt tcacggtctg ggtgtcgatg
    18541 cctatgacag acaaacctgg ggccgtgcac cgcaggctga agtcttaaca ccgggctata
    18601 agtacaatct gaccgatatt aacgccgcga ttgccctgac acagttagtc aaattagagc
    18661 acctcaacac ccgtcggcgc gaaattgccc agcaatatca gcaagcactg gcagctctcc
    18721 cctttcagcc attaagcctt cccgcctggc cgcacgttca cgcctggcat ctgtttatta
    18781 ttcgtgtcga tgaacaacgt tgtggtatca gtcgcgatgc gttgatggaa gcgttaaaag
    18841 aaagaggcat tggtaccggg ttacatttcc gcgccgctca cacacaaaaa tattatcgcg
    18901 agcgttttcc cacgctgtcg ttaccgaata ccgaatggaa tagcgaacgc atctgttcgt
    18961 tgccgctgtt cccggatatg actaccgccg atgccgaccg cgtcatcaca gcccttcagc
    19021 aactcgcagg acaataagcc atgtttgaaa tccaccctgt taagaaagtc tcggtggtta
    19081 ttcccgttta taacgagcag gaaagcttac cggaattaat caggcgcacc accacagcct
    19141 gtgaatcgtt ggggaaagag tatgagatcc tgctgattga tgacggcagt agcgataatt
    19201 ccgcgcatat gctggtcgaa gcctcacaag cggagaacag ccatattgtg tctattttga
    19261 ttaaccgcaa ttacgggcaa cattcagcga ttatggcggg attcagtcac gttactggcg
    19321 acttaattat tacccttgat gccgatctcc agaatccgcc agaagaaatc ccccgcctgg
    19381 tggcaaaagc cgatgaaggt tacgacgtgg tagggactgt acgccagaac cgccaggaca
    19441 gctggtttcg taaaacagct tcgaagatga ttaaccggct tattcagcgc accactggca
    19501 aagcgatggg tgactacggt tgtatgctgc gcgcctatcg ccgtcatatt gtcgatgcga
    19561 tgttgcactg ccatgaacgc agcaccttta tcccgattct ggcgaatatc ttcgcccgcc
    19621 gtgcaattga aattccagta catcatgccg agcgtgagtt tggtgaatcc aaatacagtt
    19681 ttatgcgcct gattaatttg atgtacgacc tggtgacctg ccttaccaca acgccgctac
    19741 gtatgctgag tctgctcggc agcattattg cgattggagg ttttagcatt gcggtgctgc
    19801 tggtgatttt acgcctgacc ttcggaccac aatgggcggc agaaggcgtc tttatgctat
    19861 ttgccgtgct gtttactttt attggcgctc agtttatcgg catgggatta ctcggtgaat
    19921 atatcggcag gatctacacc gatgtccgcg cccgcccccg ctattttgtt cagcaagtta
    19981 tccgtccatc cagcaaggaa aatgaataat gaaaaccgtc gtttttgcct accacgatat
    20041 gggatgcctc ggtattgaag ctctgctggc tgccggttac gaaattagcg ccatttttac
    20101 ccataccgat aatcccggtg aaaaagcctt ttatggttcg gtggctcatc tggcggcgga
    20161 aagagacatt ccggtttatg cgccggataa cgttaatcat ccgctgtggg tggaacgcat
    20221 tgcccaactg tcaccagagg tgattttctc tttttattat cgccatctta tttgcgacga
    20281 gatttttcag ctcgctcccg caggtgcatt taatctgcac ggttcgctgt taccaaaata
    20341 tcgtggtcgc gcgccgctga actgggtgct ggtgaacggt gaaacggaaa cgggcgttac
    20401 attgcaccga atggtaaacg tgccgatgct ggggccattg tggcgcaact gcgcgttgcc
    20461 attgcgccag acgatatcgc tattacgctg catcataaat tgtgccatgc cgcgcgccag
    20521 ctactggaac agacattacc cgccattaaa cacggtaata ttctggaaat cgcccagcgc
    20581 gaaaacgaag ccacctgttt tggtcgcaga acgccggatg acagtttcct tgaatggcat
    20641 aaaccggcat ccgtactgca caacatggta cgtgccgttg ccgatccgtg gccgggtgcc
    20701 ttcagctatg ttggcaatca gaaattcacc gtctggtcgt cgcgtgttca tcctcatgcc
    20761 agcaaagcac agccggggag cgtgatttct gttgcgccac tgctgattgc ctgtggcgat
    20821 ggcgcgctgg aaatcgtcac cggacaggcg ggcgacggca ttactatgca gggctcgcaa
    20881 ttagcgcaga tgctgggcct ggtgcaaggt tcacgcttga atagccagcc tgcctgcacc
    20941 gcccgacgcc gtacccgggt actcatcctc ggggtgaatg gctttattgg caaccatctg
    21001 acagaacgcc tgctgcgcga agatcattat gaagtttacg gtctggatat tggcagcgat
    21061 gcgataagcc gttttctgaa tcatccacat tttcactttg tcgaaggcga tatcagtatt
    21121 cattccgaat ggattgagta tcacgtcaaa aaatgtgatg tcgtcttgcc gctggtggcg
    21181 atagccacgc cgattgaata tacccgcaac ccgctgcgcg tatttgaact cgattttgaa
    21241 gagaatctgc gcattatccg ctactgcgtt aagtaccgta agcgaatcat cttcccgtca
    21301 acttcagaag tttatgggat gtgtagcgat aaatacttcg atgaggacca ttctaattta
    21361 atcgtcggcc cggtgaataa accacgctgg atttattcgg tgtcaaaaca attacttgat
    21421 cgggtgatct gggcctatgg cgaaaaagag ggtttacagt tcaccctctt cctcccgttt
    21481 aactggatgg gaccacgact ggataacctt aatgcagcgc gaattggcag ctcccgcgct
    21541 attacgcaac tcattctcaa tctggtagaa ggttcaccaa ttaagctaat tgatggcgga
    21601 aaacaaaaac gctgctttac tgatatccgg gacggtatcg aggcgttgta ccacattatc
    21661 gaaaatgcgg gaaatcgctg cgacggcgaa attatcaaca ttggcaatcc tgagaacgaa
    21721 gcgagcattg aggaactggg cgagatgctg ctggcgagct tcgaaaaaca tccgctgcgc
    21781 catcatttcc caccgtttgc gggctttcgc gttgtcgaaa gtagctgcta ctacggcaaa
    21841 ggatatcagg acgtagagca tcgtaaaccg agcatccgca atgcccaccg ctgcctggac
    21901 tgggagccga aaattgatat gcaggaaacc atcgacgaaa cgctggattt cttcctgcgc
    21961 accgttgatc ttacggataa accatcatga ccaaagtagg cttacgcatt gatgtcgatg
    22021 cctttcgtgg cacccgtgaa ggcgtgccgc gtctgctgga aatcttgagt aagcataata
    22081 ttcaggccag catttttttc agcgtcggcc cggacaatat gggccgccat ctctggcgac
    22141 tggtgaagcc acagtttttg tggaagatgc tgcgctcaaa cgcggcatcg ctttatggct
    22201 gggatatttt actggcaggt acggcctggc caggtaaaga gattggtcat gccaatgccg
    22261 atatcattcg tgaagcggct aaacatcacg aagtcggtct gcacgcctgg gatcaccatg
    22321 cctggcaagc ccgtagcggt aactgggatc ggcaaacaat gatcgacgat attgcacgcg
    22381 gtcttcgcac tctggaagag attatcggtc aaccggtaac ctgttctgcc gctgcgggct
    22441 ggcgtgccga ccagaaggtg atcgaagcaa aagaagcgtt ccatttgcgc tacaacagcg
    22501 attgtcgtgg ggccataccg ttccgtccac tgcttgaatc aggaaaccct ggcactgcgc
    22561 aaattccggt gaccttaccc acctgggatg aagtgattgg tcgggatgtg aaagcagaag
    22621 attttaacgg ttggttactc aaccgcatcc tgcgagataa aggcacgccg gtttatacca
    22681 ttcatgcaga agttgaaggc tgcgcttatc agcataattt tgtggatctc ctcaaacgcg
    22741 cagctcagga aggcgtgaca ttttgccctt taagcgaact gttatcagag acgttgccgc
    22801 tcggacaagt tgttcgcgga aatattgccg gacgtgaagg ctggctgggt tgccaacaaa
    22861 ttgcgggtag tcgctgatga aatcggtacg ttaccttatc ggcatcttcg cgtttattgc
    22921 ctgctattac ctgttaccga tcagcacgcg tctgctctgg caacccgatg aaacgcgtta
    22981 tgcggaaatc agtcgagaaa tgctggcatc cggcgactgg attgtgcccc atctgttagg
    23041 gctacgttat ttcgaaaaac ccattgccgg atactggatt aacagcattg ggcaatggct
    23101 atttggcgcg aataactttg gtgtgcgggc aggcgttatc tttgcgaccc tgttaactgc
    23161 cgcgctggtg acctggttta ctctgcgctt atggcgcgat aaacgtctgg ctctactcgc
    23221 cacagtaatt tatctctcat tgtttattgt ctatgccatc ggcacttatg ccgtgctcga
    23281 tccgtttatt gcattctggc tggtggcggg aatgtgcagc ttctggctgg caatgcaggc
    23341 acagacgtgg aaaggcaaaa gcgcaggatt tttactgctg ggaatcacct gcggcatggg
    23401 ggtgatgacc aaaggttttc tcgcccttgc cgtgccggta ttaagcgtgc tgccatgggt
    23461 aacgacgcaa aaacgctgga aagatctctt tatttacggc tggctggcgg ttatcagttg
    23521 cgtactgacg gttctcccct ggggactggc gatagcgcag cgggagcctg acttctggca
    23581 ctattttttc tgggttgagc atattcaacg ctttgcacta gatgatgccc aacatagagc
    23641 tccgttctgg tactacgtgc cggtcatcat tgccggtagc ctgccgtggc tgggattact
    23701 ccccggtgca ctgtacacag gctggaaaaa ccgcaagcat tccgcaaccg tctatttgtt
    23761 gagctggacg ataatgccgc tgctgttttt ctccgtcgct aaaggcaaat tgcccaccta
    23821 tattctttct tgctttgcac ctctggcaat gctgttggcg cattacgcgt tgctggcagc
    23881 aaaaaataat cctctggcgc tgcggattaa tggctggatt aacatcgctt ttggcgtcac
    23941 tggcattatt gccacatttg tgatctcccc ctggggacca atgaacacgc cggtgtggca
    24001 aaccttcgag agctataaag tcttttgtgc ctggtcgatt ttttcgctat gggcattttt
    24061 cggctggtac accttaacaa acgtcgaaaa gacctggtct ttcgccgcgc tttgcccgct
    24121 ggggctggcg ttgctggtag gattttcaat tcctgacaga gtcatggaag gaaaacatcc
    24181 gcaatttttt gtcgagatga cacaagaatc acttcagcca agccgctata ttcttactga
    24241 tagcgtcggt gttgccgcag gtctggcatg gagcctgcaa cgcgatgaca tcatcatgta
    24301 tcgccagaca ggtgagttga aatacggcct taattatccg gatgcgaaag ggagatttgt
    24361 cagcggtgat gagttcgcaa actggcttaa tcaacatcgt caggagggga ttattacact
    24421 cgtgctttcg gttgaccgcg atgaagatat caacagtctc gccattccgc ccgcagatgc
    24481 catcgttcgt caggagcgtc tggtgctgat tcagtatcgt cccaaatgat ctggctaaca
    24541 ttagtctttg ccagcttgct tagcgttgcc gggcagttgt gtcagaaaca ggcaacctgc
    24601 tttgtggcga taaacaaacg gcgcaaacat atcgtgctgt ggctgggact ggcgctggct
    24661 tgtcttggtc ttgccatgat gctctggctg ctggtcttgc agaacgtacc ggtaggcatt
    24721 gcctacccga tgttaagtct gaattttgtc tgggtgacgc tggctgcagt aaaactgtgg
    24781 cacgaaccgg tatcgccgcg tcactggtgt ggggtggcgt tcattattgg tggcattgtg
    24841 atcctcggga gtacggtgta atgtgcctga tatggggatt attcagcgtc attattgcct
    24901 cagttgcgca attaagcctg ggttttgcgg caagtcatct gccgccgatg acgcacttat
    24961 gggattttat cgcggctctg ttagcttttg gcctggatgc ccggatactg ttactcgggc
    25021 tgctgggtta tctgctttcc gttttttgtt ggtataaaac gctacataaa cttgcgctaa
    25081 gcaaagccta tgccttgctc agtatgagtt atgtgctggt atggattgct tcaatggtac
    25141 ttcccggctg ggaagggact ttttcgctga aagcactact gggagtagcc tgtattatga
    25201 gcgggttgat gctgattttc ctgcccacga caaaacaacg ttactgagtt ttccctgccg
    25261 ctgtacactg ccgttcccac tcatcggggc tataactgct tgccgagagg actttcaccg
    25321 tatgcagctt ttcacgatta atacaataaa gcgcattctg caaaggcgag agtaaatccc
    25381 ccactttcac ggcgaagtca ctcttcactt cggcgatcat ttttatcgcg ccgccagcat
    25441 cgcagagcat aagtacatgt ctgttatctt gtttattgca gcacgatttt ttgtgccagc
    25501 cattccattg cattatcctg tttgctaaga gttttcacat caatttacag attaacagat
    25561 aaactcttta tcaacggcaa tggaatggca gaaatgagaa gtgaaacgga gggttaatta
    25621 ttgctgacgt tgcacccact cttttagcgc ctgacgtgaa attttaatac cgccgttttt
    25681 cagctccggc ggtaaagtca gccagcgcac cggttgttga aaacgagcca gcttatcttt
    25741 cacccattcg ccaagatcaa cggtttgctg gtcatactcc accaccgcta ccggccgatg
    25801 accaaactcc ttgtcggcaa cggaaacgat aaacacctgc aaaaccgcag gatgtgcagc
    25861 aataacacgc tcgacttctt ccggctgaat accctcaccg ccactgaaga acagattgtc
    25921 taaacgtccg acaatggtca gcttgccatt atgcatctca ccgcgatcgc gcgtagcgta
    25981 ccagccttcg tcattaacca gtgaaaccag ttgcccgtta cgccagtaac cttctgccat
    26041 actggcagcc cgcagccaca cttcattatt aacgattttc acttcccgac ccggcagcgg
    26101 caaaccaacg tctgccaggc cgtcggcttc tttcgcacat accgtggagg caaactcggt
    26161 cagaccatag ccgcaaaagc aacgaatccc ctgctcgcgc gcctgttccg tcaactcgac
    26221 cgggatagcc gcgccgccaa gtaacaccgc tttcagggaa acggaactgc ggttaaccag
    26281 caaacgccag agttgtgttg gcaccagtga agcgtgagta cagcctgcca gcatttgctc
    26341 caatggctgt ttatcacgca ccgtcatccg cgcaccagcg tataaccagc gccacataat
    26401 tccctgaccg gagacgtgaa acagcggtaa agagagcaac cagtcatcgt gatcgccaaa
    26461 cggaatcagc gataacacac cttgcgcact ggcaagatgg gcctgataag tatggacagc
    26521 ggctttcggc aaaccggtag aacccgaggt caacgtcatt gagcacagac gcgtcggctg
    26581 ccacgcagcg gcatgtacgc cttcaaccag ctgaatgtgc agcgacgtta atgccggaaa
    26641 cgtgttttcc ccatccggca ccagcgcaaa ttgcagcgtc agattgggca gcaattcttc
    26701 aagcaacggt tgcggcagct gagggttcac gggcaacacc cgcgccccgc attgcagtaa
    26761 cgccagccag gcgagcagcg tttgcggcgt attccacgcc cgcaacatca cgccgctgcc
    26821 ctcaaccacc ccctgcgccg caaagcccgt agctaactca tcgacgcgag cacaaagctc
    26881 gcgccagttg agttgctcgt cattaagacg taaggcgatg gcttctcccc gcacttgccg
    26941 ccagtgacgc cacggccagt cagagaagat cataacaact gctcctgtac atcagcgtcc
    27001 actaacggca gtgggctccc cggccagcga cgaacctgct gcgcctgcat cagatccagc
    27061 gtgtccagtc ccggaatggt gtctggcgtt aaccaggcag caatccgcgc cagttgcgtt
    27121 agacctaagc tcgactcaat ggaagaactg atcaccgccg tcagccccag cgcatgcgcc
    27181 gcctgtacct gctcgcgtac tttttccaga ctgcccgtga gcgtgggttt gataactacc
    27241 gcgcgcacgc cctcttcagc cacaaaggca aaatccggct cgcgcaggct ttcatcccag
    27301 gcaatggcaa tgccggtttc acgggcaaac gctcgcgaat catcgcgggt tttgcacggc
    27361 tcttcgagaa acgcgatgcg gtggcgataa tccgggttaa catatttggc aaactgctga
    27421 cctttcagcg gtgtccaggc gcgatttgcg tcaagacgca aatgcagatc cggaattgcc
    27481 tccagcaaca gattcaccac catgccgtcg cgcaccgctt cgtacaatcc cactttgacc
    27541 ttcgccactt tctcgcccgg catatcggca agtttgagga tcagatcgtc cggatcgcca
    27601 ttacacagcg gtgccgcacg gtagttggct gcttgcggca acgtatctgt cagttctgcc
    27661 aatgcacagc ttacgccaaa ggccacggaa ggcatctgcg gtagctcgca atcgcctgcc
    27721 agccagttat ttacccaggc aagcagcacg ctttgcgcct cttcccagtt ttcctggctg
    27781 aagcccggca gtggggagat ctccccccac ccttcgcgct cgccctcacg caggcaaacg
    27841 tacagcccgt cgcgggtttt taaccgcctg tcgcgcagaa ccacccccgc gtccatgggg
    27901 atctgccagc ggtatacctg cgcgctacgc attacggatt ccgtttaaat ttgctgaaat
    27961 caggctgacg tttctggttg aaagcgttgc gaccttcctg accttcttcc gtcatgtaga
    28021 acagcatggt ggcgttgccc gccagctcct gcagccccgc ctgcccgtca cagtcggcgt
    28081 tcagcgcagc tttcaggcag cgcagcgcca tcgggctgtt ttgcagcatt tcgcggcacc
    28141 aacggacggt ttctttttcc agatccgcca gcggtaccac ggtgttgaca aggcccatat
    28201 ccagcgcctg ttttgcgtcg tactgacggc acaggaacca gatttctcgc gcttttttct
    28261 gcccgacgat acgagccatg taggaagcgc cccagccgcc gtcgaaggaa ccgactttcg
    28321 ggccagtctg accgaagatg gcattatctg ctgcgatagt caggtcgcac atcatgtgca
    28381 gaacgtgacc accgccgatg gagtagccag ccaccatcgc gacaaccggt ttcggacagg
    28441 tacggatctg acgctggaag tccagcacgt tcaggtgatg tacgccggaa tcatctttat
    28501 agccgccgta atcaccacgc actttctggt caccaccgga gcagaacgct ttatcgcctg
    28561 cgccagtcag aatgatcacg ccgatattgt cgtcataacg cgcatctgcc agtgcctgga
    28621 tcatctcttt taccgtcaga ggacggaagg cattgcgcac ctgcggacga ttaatggtga
    28681 tttttgcgat accgtcggtg gatttttcat aacgaatgtc ctcgaaacct tcggagcagt
    28741 cgtgccattc aaccggtgcg taaggcattg cttcatcagg ataaatcata ttgtgtcctt
    28801 tagtcagaaa cgcaagatct gcgccagact tgcgattacg ccagcgggat tttcccgatg
    28861 cgcgttatgt ccggcgcgag gaatgacatg gcagtcggca gccagttccg ccgccagggc
    28921 gcggaatttg ctgtcacgtt caccacataa ataataaaac gcaaatgtgc gggcgctaag
    28981 gttagcacgt aaatcaggct ggacggcgag agaagtcgcc tccagcatgg cggcaagggt
    29041 tgcgccatta ttgttgctgc gcagcgccac cagctcccgg cgttgatcgt cattgagcga
    29101 ggcaaaaaca ggctgttgat accagtcggc aaataccgcc gttaacggtt ctgtgcgaaa
    29161 acgctgcgcc cattggcgat cggaacgctg acgttccgca cgttgttcag cattttgcag
    29221 ccccggatgc ccgccttcga caataacccc acaaagcccc gccagcccct ggcaagccgc
    29281 catcatcgcc acccgtccgc caagcgagta ccccaccagc cagaagtcaa ggatgttgta
    29341 actaaccaag gttttacgca gtaagtcggt gacatcatca aatccatcga cgctaatcgc
    29401 cgccgaacca ccatgaccta ggagatcaac atacaaccgt gagtagtcgg caaacgcctc
    29461 gcccacttct tgccattcgt ggcaatcgcc ggaaaaaccg tgcaaaaaca ccagccaggg
    29521 taaacctagt tttccgtgtt ttgcctgcgc gtgcaggatc ataaatggct tacctgcgcc
    29581 agaagttgct ggagcgtttg cgcaccatcg gtgtcgttaa ccaccatttc aatcaccgtg
    29641 gtggttggcg tgcgccaggc gtcggcaaat gccgtttcaa gttcctgcca gttttgcgga
    29701 tgatgatatt tcagctcgaa catcgcggcg gcgtgctcaa aatggacgtt ttgcggcatc
    29761 agatagaaac gctcacgctc gctttgcggc gttggcaaca gcgagaaaat ttgcccgccg
    29821 ttgttgttca ccacaattaa taccagcggc gcagaaacct gacgcaataa cgccagcgcg
    29881 ttgagatcgt aaagcgcgga gagatcgccc acaatcgcca gcgtcggttt gccgcttgcc
    29941 cgctgaacgc ctgcggcggt cgaaagcagt ccgtcgatac cgctggcccc tcggttgctg
    30001 tacaccgggt aacctgccgg aagttgcgaa agcgcatcaa tcagacgtac caccaggctg
    30061 ttaccaacaa acaattgccc ctgttcaggc agatagtcgc tgatgcgatg cgccagttgc
    30121 gcttcgccaa acgcatcacg gcgggcaata accgcctgca ttgcctgttc cgccaggcgc
    30181 gggatttcaa cgcaccaggg ctggcgtttt tctgccggat gcagctccag ccagtcggca
    30241 atattggcaa ttaagcgacg tccgcgatgg tgtgccggat caagtcgccc ttcaatgtca
    30301 tcaacaatcc agtactcttc tggttcacag cttgcctgcc attgcaggag ccgtttcccc
    30361 gtcaggctac ttcccagttg caccacaatt tgcgcctgct gcagctcgct ggtcgctttg
    30421 gcattgccta accaaagatc ggcacacggc agcggctgcc cggtttgtga cagcacatcg
    30481 ccaatcagcg gccagccaag agtttgcgcc cacagggcaa cttttttgcc ctcttccgca
    30541 ctcatgcgcc cggcaaccac cacgccgcgc ttttgtcgcc agaagaacca gtcgcgctgt
    30601 ttttcacttt ccagacgagg cgcttcacgc agccacggtt tgtcgtcctg ccaccagtca
    30661 cccagacgct gttgccagct aagcccggta tcgtccattt cgccatacag cggttcagca
    30721 aacgggcagt tgatatggac tccccccgca tgaagcgtac cgagagcgtg gtcgatggta
    30781 gaaaccagcc aacgtgcggg gatatcctgg gtcgggcgcg gcaatgaaat actgtgcgtg
    30841 gggtgagagg cgaacattcc cggctggcga attgcctgat tcgcgccgca gtcaattagc
    30901 tccggcgggc gatcggcggt taagagaatc agtttttctc cggttaaccc ggcttcaatc
    30961 agtgccggat agagatttgc caccgccgtg ccggaggtca caatcaccgc caccggctgc
    31021 ttgctgactt tcgccagccc cagcgccaga tgccccaacc cacgctcatc gaaatgggtg
    31081 tggtgaatga atgcggaatt ctccgccgcc gctaacgtta acggtgtaga acgcgagcct
    31141 ggggcgatac agatgtgtct gacgccgtga cgcgttaatg cttccagaat gaccgccgcc
    31201 cagcgtcggt taaatgcgct tactgacatg agtttgtccg gtatcaatat tgcggctaag
    31261 tataaggagc tacaaaaata gattattgat atgaatcggt aatgatgcga ctcattacta
    31321 ttccatttgt aataaagtac gcagccctgc cgctttgttg tcgatttcct gccactcttg
    31381 ctcggggtcg gaaccacgga caatgcccgc gccagcatat aatcgcacga cattgccgct
    31441 aatttttgct gagcgcaggg aaacgcagaa ttcgctttgt tgtaatgaga gatagcccgc
    31501 agaaccggcg taccattctc gggtgaacgg ttcgtgacgg gcgataaact gtcgcgccag
    31561 atcgcgcggt aagccagcaa ctgctgccgt tggcagcaac tgatgtaaac agatcacatc
    31621 atccgctttg ttgagtgaag tccagataca gcggcgaaga tgctgcactt tacgcagacg
    31681 cagtacctgc ggcggtaaaa catccagcgt ctgggtatcg gcctgtaatc gttggcagat
    31741 atcttccacc accagcatgt tctcgcgctg gtttttatca tccgccatca gccactctcc
    31801 taactgctgc gcctgcttat catcaggatg atttgctact gttcccgcca gcgcttcagt
    31861 acgcagcgct ttgtcacgcc gccgccataa ccgttccggt gaagagccaa gaaaagcatt
    31921 ttcgccatca aaggccatgt aaaaatggta gcaattcaga ttcagtcgac gactggcagc
    31981 catcatcgcc gccgcgttga ccggacttgc gaaatgcagg tcagttgccc gagcgagcac
    32041 cactttgtcg agctcacctt cggcgatggt tttcgttgcc agttcgatta attgcgtcca
    32101 gcccgttttg tccggccagt gttgttctcg cgtggtggtt aaatgtaacc caggcaaggg
    32161 cttgatactc accagtgtgg cgataaattc ttttgcctga atcgcatcgt gctgaaggga
    32221 gctttcgctg aatagcgtca gccgcagcgt ggctttaccg ccacagcgtc gccattccag
    32281 gcggggtaaa agtaaattgc cctgcgacgg gtcaaaagca ttcagccccc aaatgcgtaa
    32341 gtcagcgtgt tccgggtgct ggcgaagaaa acgttgtgcc tggtccaacg acgtaaaacg
    32401 ggtaatcgcg cccaggacgg cagcttcttc atcaccatta cgttgttgcc agtagaattg
    32461 cgggtacgtt tgctgactgg ccagccagct caaggcatca aaagcgtctt tgagagggaa
    32521 aggaatatcg ataacccgaa tgccgggtgt cgccggaatc tcttgcgaca aatggcgcag
    32581 tagattttcc agcgccgtag taagtgattg cacgcggacc tctccctgtt aaaagcctca
    32641 cattatacgg ggtactacaa aaaaaatgca gtaccccggt gtagggaggt tttaacggcg
    32701 tgccagcaac agtcctagta ccagcccgac ggccgcgccc acaccaattc cttgccaggg
    32761 tttttcgtgg acgtagtcat cagcacgata aacagcctgc ttcgcccgat agtaataact
    32821 gtctgaagcc tggctaaccc gttttttcac atcatccagc gctttttctg catgcgcttt
    32881 cagctcaaca tatttctgat cggcgggatc gccagaggag cggagcacct cttccagtgt
    32941 ttcactaagc agcgtcaggt cgtcatcgat acgtgtatca ccaaactgat tagacattct
    33001 cgttctccat ttgcgtaaaa cctgtcacta actatagaca agagttgttg attacgcctg
    33061 aattacctcg cgtgccatcc caatgtgtgg gataccatct tcttcataga cctccgtcac
    33121 agggataaaa ccgaaactct ggtagaagtt ttgcagatgc gcctgcgccc cgaggtaaac
    33181 aggcttatca ggccagtgat gcgtacatgt ttcgagtgtt ttactcatca gctgctgccc
    33241 cactttttca ccacgcaacg cttcactgac aatcacccga cctataacga ccggctcaag
    33301 atcatcatca cttttcagaa tcctcgcata cgccaccagt tcatcatttt tccaaccgag
    33361 aatatggcga ttatcccccg tcaaatcatc accatcgata tcctgataag ggcaattctg
    33421 ctcgacaaca aacaccgcgc aacgcagttg taataaggca taaagctggg aaaccgataa
    33481 ttcagagtga tgcagatctt gccattcaat cataatgctc tccttttaga tgtatgcctt
    33541 atactagccg gaatattttt tgaaacgtga tgaactcatg gaattaattt ttttaggtac
    33601 ttcagccggt gtgccaaccc gcacgcgcaa tgtcacggca atattgctta acctgcaaca
    33661 tccgacccag agcggacttt ggttgtttga ctgcggtgaa ggcacccagc atcagctact
    33721 gcataccgcc tttaaccctg gaaaactgga caagattttt atcagtcacc ttcatggcga
    33781 tcatcttttt ggtttacccg gcttgctgtg cagtcgttct atgtcaggca ttatccaacc
    33841 cttaacgatt tatggtccgc aaggtatccg tgaatttgtg gaaaccgcgc tgcggattag
    33901 cggctcatgg accgattatc cgctggaaat tgtcgaaatt ggcgctggcg aaattctcga
    33961 tgatggcctg cgcaaagtaa ccgcttatcc gctggaacac ccactggaat gttatggcta
    34021 tcgtattgaa gaacatgata aaccgggtgc attaaatgcc caggcattaa aagctgctgg
    34081 cgtgccgcct ggcccactgt ttcaggaatt aaaagcgggc aaaacaatca cgctggaaga
    34141 tggaaggcag attaacggcg cagattactt agctgctcca gtgccaggta aagcgctcgc
    34201 tattttcggc gataccggcc cctgcgatgc cgcacttgac ctggctaaag gtgtcgatgt
    34261 catggtgcac gaagcgacgc tggatataac catggaagcc aaagccaata gtcgcggcca
    34321 tagctctaca cgccaggctg cgacactagc ccgtgaggct ggagtcggca agctaatcat
    34381 tacccacgtc agctcgcgct atgatgacaa aggttgtcaa cacctgttac gtgaatgcag
    34441 gtcaattttc ccggcgactg aactggcgaa tgatttcacc gtgtttaacg tttaactctg
    34501 cttattatta aaaacagggt gaaacttgcc ctgttatagc aacccgcgct acatatatcc
    34561 acccacacag gcatattgag cgatttttga tatttattca tctccttacg cactatattc
    34621 agagcaataa gttgcctctt ttcatcttga ttcagcacaa cctttgcaac taaaaggagc
    34681 ccatgatggt tacagttgtc agcaattatt gtcaattatc tcaaacccaa c
//
LOCUS       DAJTUI010000035        34028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTUI010000035 DAJTUI010000000
VERSION     DAJTUI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.035"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..2099
                     /locus_tag="OO464_001847"
     CDS             <1..2099
                     /locus_tag="OO464_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR6227015.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2096..3184
                     /locus_tag="OO464_001848"
     CDS             2096..3184
                     /locus_tag="OO464_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6227016.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3272..4570)
                     /gene="exuT"
                     /locus_tag="OO464_001849"
     CDS             complement(3272..4570)
                     /gene="exuT"
                     /locus_tag="OO464_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR6227017.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5055..6467
                     /gene="uxaC"
                     /locus_tag="OO464_001850"
     CDS             5055..6467
                     /gene="uxaC"
                     /locus_tag="OO464_001850"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6227018.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6482..7969
                     /gene="uxaA"
                     /locus_tag="OO464_001851"
     CDS             6482..7969
                     /gene="uxaA"
                     /locus_tag="OO464_001851"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR6227019.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8052..8603
                     /locus_tag="OO464_001852"
     CDS             8052..8603
                     /locus_tag="OO464_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6227020.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8608..9852)
                     /gene="sstT"
                     /locus_tag="OO464_001853"
     CDS             complement(8608..9852)
                     /gene="sstT"
                     /locus_tag="OO464_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6227021.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10448..11413)
                     /gene="alx"
                     /locus_tag="OO464_001854"
     CDS             complement(10448..11413)
                     /gene="alx"
                     /locus_tag="OO464_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6227022.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11684..12682)
                     /locus_tag="OO464_001855"
     CDS             complement(11684..12682)
                     /locus_tag="OO464_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6227023.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12761..13453)
                     /locus_tag="OO464_001856"
     CDS             complement(12761..13453)
                     /locus_tag="OO464_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6227024.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13530..14033)
                     /locus_tag="OO464_001857"
     CDS             complement(13530..14033)
                     /locus_tag="OO464_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6227025.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14118..15254
                     /gene="rlmG"
                     /locus_tag="OO464_001858"
     CDS             14118..15254
                     /gene="rlmG"
                     /locus_tag="OO464_001858"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6227026.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15539..15853
                     /gene="higB"
                     /locus_tag="OO464_001859"
     CDS             15539..15853
                     /gene="higB"
                     /locus_tag="OO464_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6227027.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15850..16266
                     /gene="higA"
                     /locus_tag="OO464_001860"
     CDS             15850..16266
                     /gene="higA"
                     /locus_tag="OO464_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6227028.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16311..18329)
                     /gene="fadH"
                     /locus_tag="OO464_001861"
     CDS             complement(16311..18329)
                     /gene="fadH"
                     /locus_tag="OO464_001861"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6227029.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18555..20906)
                     /gene="ygjK"
                     /locus_tag="OO464_001862"
     CDS             complement(18555..20906)
                     /gene="ygjK"
                     /locus_tag="OO464_001862"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR6227030.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20923..21342)
                     /locus_tag="OO464_001863"
     CDS             complement(20923..21342)
                     /locus_tag="OO464_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227031.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21320..21673)
                     /locus_tag="OO464_001864"
     CDS             complement(21320..21673)
                     /locus_tag="OO464_001864"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227032.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21674..21994)
                     /locus_tag="OO464_001865"
     CDS             complement(21674..21994)
                     /locus_tag="OO464_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227033.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22128..23561)
                     /locus_tag="OO464_001866"
     CDS             complement(22128..23561)
                     /locus_tag="OO464_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6227034.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23624..24073)
                     /locus_tag="OO464_001867"
     CDS             complement(23624..24073)
                     /locus_tag="OO464_001867"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6227035.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24070..27156)
                     /gene="ebgA"
                     /locus_tag="OO464_001868"
     CDS             complement(24070..27156)
                     /gene="ebgA"
                     /locus_tag="OO464_001868"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR6227036.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27340..28323)
                     /gene="ebgR"
                     /locus_tag="OO464_001869"
     CDS             complement(27340..28323)
                     /gene="ebgR"
                     /locus_tag="OO464_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6227037.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28541..28872
                     /locus_tag="OO464_001870"
                     /pseudo
     CDS             28541..28872
                     /locus_tag="OO464_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28914..30293)
                     /gene="ygjG"
                     /locus_tag="OO464_001871"
     CDS             complement(28914..30293)
                     /gene="ygjG"
                     /locus_tag="OO464_001871"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6227038.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30711..32231
                     /gene="aer"
                     /locus_tag="OO464_001872"
                     /pseudo
     CDS             30711..32231
                     /gene="aer"
                     /locus_tag="OO464_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32385..33008)
                     /gene="nfeR"
                     /locus_tag="OO464_001873"
                     /gene_synonym="yqjI"
     CDS             complement(32385..33008)
                     /gene="nfeR"
                     /locus_tag="OO464_001873"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6227039.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33285..34028
                     /gene="nfeF"
                     /locus_tag="OO464_001874"
                     /pseudo
     CDS             33285..34028
                     /gene="nfeF"
                     /locus_tag="OO464_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
BASE COUNT         8297 a         8773 c         8930 g         8028 t
ORIGIN      
        1 atttcaacgg ttatgatgca gatgatgtca gtgacagtaa tactgacgtc agcgatctct
       61 attatcgtta tgatttttta cgtcgttatt atgtgcaggc gggtcgcatg gacaaccgca
      121 cactatttaa tgcacaaggc gggaacttta cctttaactt tctgccactc ggtgcaatcg
      181 atgggatgcg tatcgggtcg acactgagct atttaaacca ggcgcaaagc cagcaaggaa
      241 ccccagtaat ggttctgctt tcgcgcaatt ctcgtgttga cgcttatcgt aatgagcaac
      301 ttttgggatc gttttatctc aatagtggtt cgcaatttat tgataccagt tcctttccgc
      361 caggcagcta cagcgtggca ttaaaagtct acgaaaataa ccaactcacc cgcaccgagc
      421 ttgtgccgtt taccaaaacc ggcggtctga ctgacggaaa tgcgcaatgg ttcttacagg
      481 caggtaaaac tacatcacag gtttctgatg atgaaagttc agcttatcag ctaggagtac
      541 gcctgccatt acatccgcaa tatgagctct acgcagggct ggcgaatgct gatgatgtga
      601 gtgctttcga gttaggtaat aactggacgg cagatttagg cagggtgggg aatcttgcaa
      661 tcagcgccag cgtgttccgt aacgatgacg gcggcaaagg tgatatgcaa caggccaact
      721 ggagtaatcc gggatggccg acgttgggct tttatcggac caactctgac ggcgatgctt
      781 gtacaaccga cagcagagag agctataacg ccttaagctg ttatgaaagt atttccgcga
      841 cagtttcact gaattttgtc ggctggaata tgatgctggg ttatacctgc acacaaaata
      901 acactgatga tagtttgcgt tgggataaac agcagagctt tgaaaataac tatcttcgcc
      961 agacaacagc gcaaagtatc tctgaaactg tacaacttag cgcttcccgc gctattgtga
     1021 tgcgtgactg gattttgagt acgtcagtcg gtgttttcca tcgtaatgac aacggtggcg
     1081 ataacgacga caacggcttg tacttatcgt tttcgttatc tgacacgcca acgatggata
     1141 gcaataacaa cagccattca accaatgtgc ctacggatta tcgttatagc gaacaggatg
     1201 gcgatcaaac gtcatggcag ttatcgcata ccttttataa cgattcattc agccataaag
     1261 aacttggcgt aaccgtcgga ggcctgaaca ccgataccat aaacagcgcg gttaacgggc
     1321 gttgggatgg gcaatacgga aatgtctacg ctaccgtatc tgatagttat gaccgtaaga
     1381 atcatgatca tctttcggcc tttacgggca cttatagctc tacgctggct gtcagtcgct
     1441 atggcgttaa tttgggagcc agtggtacag acgatttgct gggtgcggta ctggtggatg
     1501 tgaaaggctt ctctgaacag gatgaagaga gtcaggatct gcaactcgaa gcgcgggtgg
     1561 caggcagccg aactttgcag cttggtcaaa gtgacagtgt gttgttccct tatcctggat
     1621 ttcagtctgg ttttgttgag gttaacgaca gtagccaggg caatcagcag gggacaacaa
     1681 acatcattaa cggtgcaggg aatcgtgaat taatgttgtt gcctggcaag ctgcgctatc
     1741 gcgaagtgtc tgccagcttt aactacaact atatcggtcg tttattactg ccggcagcgg
     1801 tgaaaaaatt cccgattgtg gggctgaaca gcgccatgtt actggtagct gaagatggcg
     1861 gatttacact tgaaattaac ggcagcgaaa aagagctcta tctgctttcc gggcagcaat
     1921 tccttaagtg tccactgagt gttgtaaaga aacgcgccag cattcgttac agcggagatg
     1981 ttacttgtag tgtggtgact tattcacaat taccggagtc gattcaggtt caggcacagt
     2041 tgaaacagcc taaattacgt ggaaacgttc agacggcgca aagggaggtt gcaccatgag
     2101 aaatcgattg attgcggcga tattgggttt gtttggcacg ctcactggcg ttcaggcagc
     2161 tcctgacgtg accagtgaaa ttacgtatga tttggcatct ggtagagcgg attattactt
     2221 ctggaaggat gaagcgtcgg caggaaataa tggatatatg tggtatgaat gttcgtatcc
     2281 tgacctccaa caaacctgta cagctaatgg aaatatatcg acagtacaaa tctatttaac
     2341 tgaacaacgc agtgggatgc gttggccggt aaaactcaaa ggatttaaaa cagccattgt
     2401 aagtagcgat gaagcgccgc taggatgcaa ggggggcaaa gggcttcaga cgaatcttaa
     2461 ggattctaat agatcttcat gtacagaaga tggtcaacat tattatatat acgatacaaa
     2521 gtttcttacg ctctaccttg agcagacaga gatgaagaat ttgccgattg gtggcgtctg
     2581 gaaggggaaa gttaaattac attcgaacag cccggcccag gactatttcg caaatattac
     2641 cctgaatacg ctcgacccca accatattga cgtgttcttc ccggagttcg cccacgccac
     2701 gccaagggtg cagttagact tgcatccaac aggaagcgtt aacggcagca actacgcgca
     2761 agatctgacc atgttggaca tgtgcctgta cgatggtttt aacggtaatg ccatcagtta
     2821 tgaaatcatg ctcaaagatg aagggcgacc agctgcaggg cgcagagacg gttacttctc
     2881 tatctatcgt cagggaggga ccaccaccga cgagggagaa cgcattgatt accgggtcaa
     2941 aatgtacaac ccggaaaccg gtgggcaaat tgatgtgcgc aataatgaaa atatggtctg
     3001 gaacagcatt aacctgaaac gtgtgcgtcc ggtggtactg cccggtattc gctatgccgt
     3061 gatgtgtgtg ccgacaccct tgactctggc agtcgataaa ttcagcgtga tggataaaca
     3121 ggccgggtac tacatgggca aattgtcagt aatctttacg ccttccttgc caaccatcaa
     3181 ttaaccctgc aaaccggaca aacgcttttt tctatataaa cgacgaagcc gcctgataag
     3241 gcggcttcgt cgttataact tacgttaaac gttaatgttg cggtgcggga tcgttatgtg
     3301 tttcctgcgc cacctcaatc gccggtttgt tctgcaatac ggtccagata accagcgcac
     3361 ccagcaggtc aaatactgcc agaactgcga acagcgggct gaagccgatg gtgtcagcca
     3421 gtgcaccgac aaccagcgca aacagcgtac ttgccagcca tgcggacatc ccggttaaac
     3481 cgttcgccgt cgccacttcg ttacgaccga acacgtcaga agagagcgta atcagcgcgc
     3541 cagacagtgc ctggtgggca aaaccaccga tacacagcag cataattgcg acatacgggt
     3601 tggtgaacag accgatcata cccgggccaa tcatcagcac agcacccagc gttacgacca
     3661 tcttacggga aacgatcagg ttcacaccaa accaacgctg gaacagcggc ggcaggtaac
     3721 caccgaggat acaaccgagg tcagcaaaca gcatcggcat ccaggcgaac atcgcgatct
     3781 ctttcaggtt aaagccgtaa actttaaaca tgaacagcgg gatccacgcg ttaaaagtac
     3841 cccaggccgg ttctgcaagg aaacgcggca gtgcgatacc ccagaactga cggttacgca
     3901 ggatctgacc aacggacatt ttcttcgccg tgctcacctg gtgctgggct tcctgaccat
     3961 taataatata gtcgcgttct tcatcggtca gatgcttctg gtcgcgcgga tgtttataga
     4021 aaatcagcca tgccatcgcc cagataaagc tcaatgcacc ggagatgata aatgccatct
     4081 gccagctgtg catcacgatt gcccatacca ccagcggcgg cgcaatcatc gcaccaatcg
     4141 aagaacctac gttaaagtag cctactgcga tggaacgctc tttcgccggg aaccattcgg
     4201 agctggcttt cagacccgcc gggatcatcg ctgcttccgc ggcaccgacc gcaccacgag
     4261 caacagctag gccaccccag ctacctgcca gcgcggttgc accacagaac acggcccaca
     4321 gcacagcaaa cattgcataa ccgattttcg tacccagcac atccagtaca taacctgcta
     4381 ccggttgcat gaccgtataa gcagcagaat aggctgcgat gatataggaa tactgttggg
     4441 tggagatgtt taactcttcc atcagagttg gcgcagctgc cgccacagtg ttacgcgtca
     4501 ggtaaccaag cacggtgccg agcgtcacca gtgcgatcat ataccaacgt aaccctttaa
     4561 ttttacgcat gtaaaacctc atcgttttgt tatatccgca ggaagcgcgg ccatcatccc
     4621 gccagaggct gaagatgtta gttacgaccg ggtaaccgaa aaaaaaaacc ggcacgcccc
     4681 gaacgttgcc atgaaagttt cgtgctaatt cggcttccgt accggttaac ccgattcatc
     4741 taacaaatga atgaatcggc aatttgcatt ccgtcgactt atgatttgcg acggcagaaa
     4801 gataacttgt catacaacct taaaaggtga gagccatcac aaatgtgcga atatttgtag
     4861 ggacattacc tgacgacagc gaggccagta ctggcgcggc ctgcagcgag atttaccact
     4921 ttgagagtaa tttttttaac tacgtttatt gatctaactc acgaaaatat cttcggactc
     4981 tggaaattgg tgtgataact ttgtcagcat cgcaccataa gcaagctagc tcactcgttg
     5041 agaggaagac gaaaatgact ccgtttatga ctgaagattt cctgttagat accgaatttg
     5101 cccgccgtct gtatcacgac tacgcaaaag accagccgat tttcgattac cattgccatt
     5161 tgccgccgca gcagattgcg gaagactatc gttttaaaaa cctgtatgac atctggttga
     5221 agggcgatca ctacaaatgg cgcgctatgc gtaccaacgg tgtggctgag cgtctgtgta
     5281 ctggtgatgc gtctgaccgt gaaaaatttg acgcctgggc ggcgactgtt ccgcacacta
     5341 tcggcaaccc gttataccac tggacgcacc tcgaactgcg tcgtccgttc ggtatcactg
     5401 gcaaattgct ttctccgtca actgccgatg aaatctggaa cgaatgtaac gaactgctgg
     5461 cgcaggataa cttctccgcg cgcggcatca tgcagcagat gaacgtgaaa atggtcggca
     5521 ccaccgatga cccgatcgat tctctggagc atcacgcaga gatcgccaaa gacggctctt
     5581 tcaccatcaa agtgctgccg agctggcgtc cggacaaagc cttcaatatc gaacaggcga
     5641 cctttaacga ctacatggcg aagctgggcg aagtttccga taccgacatt cgccgctttg
     5701 ctgacctgca aactgccttg accaaacgtc tggatcactt cgccgctcac ggctgtaaag
     5761 tgtctgacca tgcgctggac gtggtgatgt ttgctgaagc gaacgaagcg gaactggaca
     5821 gcattctggc gcgccgtctg gctggcgaaa ccctgagcga gcacgaagtg gcacagttca
     5881 aaactgcggt actggtgttc cttggtgccg aatatgcacg tcgcggctgg gtacagcagt
     5941 accacattgg cgcgctgcgt aataacaacc tgcgtcagtt caaactgctg gggccggatg
     6001 taggctttga ctccatcaac gaccgtccga tggcggaaga gttgtctaag ctgctgagca
     6061 agcagaacga agaaaacctg ctgccgaaaa ccattctcta ctgcctgaac ccgcgcgata
     6121 acgaagtgct gggcaccatg atcggtaact tccagggcga aggtatgccg ggcaaaatgc
     6181 agttcggttc cggctggtgg tttaacgatc agaaagacgg tatggaacgt cagatgaccc
     6241 aactggcgca gctcggtctg ctgagccgct ttgtcggtat gctgactgac agccgtagct
     6301 tcctgtcata cacccgtcac gaatacttcc gccgcattct gtgccagatg atcggtcgct
     6361 gggtggaagc gggcgaagca ccggcggaca tcaacctgct gggcgagatg gtgaaaaata
     6421 tttgctttaa caatgcgcgt gactacttcg ccattgaact gaactaaggt ctggggttga
     6481 tatgcaatac atcaagatcc atgcgctgga taacgtcgcg gtcgctttag cagatttggc
     6541 tgaaggcaca gaagtcagtg tcgataacca gactgttacg ctgcgccagg atgttgctcg
     6601 tggacataaa tttgcgttaa cggatatcgc aaaaggggcc aacgtcatca aatatggcct
     6661 gccgattggt tatgcattgg cggatattgc ggcgggggaa cacgtccacg cccacaatac
     6721 gcgcacgaat ctgagcgatc tggatcagta tcgctatcaa cttgattttc aggatctgcc
     6781 tgcgcaagcg gcagatcctg aagtgcagat ctatcgtcgc gctaacggcg atgtcggggt
     6841 gcgtaacgag ctgtggatcc tgccaaccgt gggctgtgtc aacggcatcg cgcggcaaat
     6901 ccagaaccgt ttcctgaaag agaccaacaa cgccgaaggt accgacggcg tgttcctctt
     6961 cagccacacc tatggctgct cacaactggg cgacgatcac atcaacaccc gcaccatgct
     7021 gcaaaacatg gtgcgccacc cgaacgcggg cgcagtgctg gtgattggtc tgggctgtga
     7081 aaacaaccag gttgccgcat tccgtgaaac gttgggcgat atcgatcctg aacgcgttca
     7141 tttcatgatc tgccaacagc aggatgatga gatcgaagcc gggatcgagc atttgcatca
     7201 gctgtataac gtgatgcgca acgataaacg cgagccaggc aaactcagcg aactgaagtt
     7261 tggtctggag tgcggtggtt ctgacggtct ttctggtatt actgctaacc cgatgctggg
     7321 gcgtttctct gactacgtga ttgctaacgg cggtactacc gtactgaccg aagtgccgga
     7381 gatgtttggc gcagagcagt tgctgatgga ccattgccgc gacgaagcaa cgtttgaaaa
     7441 actggtcacc atggtcaacg acttcaaaca gtactttatt gcccatgatc agccgatcta
     7501 cgaaaaccca tcaccgggga acaaagcggg cggtatcacc acgctggaag acaaatcact
     7561 tggctgtacc cagaaagcgg gttccagtgt cgtggttgac gtgctgcgtt acggcgagcg
     7621 tctgaaaacg ccagggctga acttgttaag tgcgccgggt aacgatgccg tagcgaccag
     7681 cgccctggcg ggtgcgggct gccatatggt gctgttcagt actggtcgcg gcacgccata
     7741 cggtggattt gtgccgacgg tgaaaatcgc caccaacagt gaactggcgg cgaagaaaaa
     7801 acactggatc gactttgacg cgggtcagct gatccacggt aaagcgatgc cgcagttgct
     7861 ggaagaattt atcgacacca tcgttgagtt tgccaacggt aagcaaacct gcaacgagcg
     7921 taacgacttc cgcgaactgg cgatcttcaa aagcggcgta acgctataaa gtcgaaatga
     7981 gttgttaata aaaaacggcg tttcataatg tgaagcgccg tttgcttttc catacctgct
     8041 aaggatttat catgaccgcg tattggctgg cccagggcgt gggtgtcatc gcctttctga
     8101 ttggtatcac aacatttttc aatcgtgacg aacgtcgctt caaaaagcag ctttcggtct
     8161 atagcgccgt tattggcgta catttttttc ttctgggcac ctatcccgct ggtgccagtg
     8221 ccatccttaa tgccattcgt acattgatta ccttacgcac gcgcagctta tgggtaatgg
     8281 cgatttttat tgtgctgact ggcggaattg gcctcgcgaa gttccatcat cctgtcgaac
     8341 tattgccggt tatcggtacg attgtcagta cctgggcgct gttccgctgt aaagggctca
     8401 ccatgcgctg cgtgatgtgg ttttcaacgt gttgctgggt gattcacaac ttctgggcgg
     8461 ggtcgatagg cggcacgatg attgagggta gttttctgct tatgaatggc ctgaatatca
     8521 ttcgtttctg gcggatgcag aaaaggggaa ttgatccgtt taaagtagag aaaacccctc
     8581 ccgccataga cgaaaggggt taaacaatta attacgcagg gcgctatttg ccagacgatc
     8641 gtcttccgcc tggcaagctg ccgcagtgaa cagcacgtca gttgaagagt tcagcgcggt
     8701 ttcgcaagag tcctgcaata cgccgataat aaagccgacg gcaaccacct gcatggcgat
     8761 atcgttcgaa ataccgaaca tattacaggc cagtgggatc agcagcaaag aaccccccgc
     8821 cacgccggat gcgccacagg cacacagaga agccactacg ctcaacagca gcgctgtggg
     8881 cagatcgacc ggaataccca gcgtattaac cgcagccagc gtcaacacgg taatggtgat
     8941 tgctgcgccc gccatattga tggtggctcc cagcggaata gaaactgaat aggtatcgcg
     9001 atccagattc agcttttcac acagcgccat attcaccgga atgttcgctg cagagctgcg
     9061 ggtgaagaag gcatacacac cgctttcgcg caggcacagc agcaccagcg ggaacgggtt
     9121 acgacgaatt ttccaccaca ccagcaatgg gttaaccacc agcgccacca gtaacataca
     9181 gccaaccagc acgaccagca gttgcgcgta gccccacagt gtggagaaac cggtggttgc
     9241 cagggtagaa gaaaccagcc caaaaatacc gaacggtgcg aagcgaatga ccagtttcac
     9301 cataaaggta acggcattcg acatatcatt gaccaggttt ttggtggtct cgttaccgtg
     9361 acgcagtgcg aagccgaggc cgatcgccca caccagaatc ccgatgtagt tacctttcag
     9421 cagggcgtcg atggggttgg aaaccatgct cattaccagt ccgcgcatca cttcgacaat
     9481 gcctgacggc ggcgaaatat cacccgcgct actggacaag tgcagggtag aagggaaggc
     9541 aaagctgaag actactgcgg ccagagctgc agagaaggtg cccagcagat agaggaacaa
     9601 aataggacgg atattggttt tctgcccgtg ctggtggtta gcaatagatg ccattaccag
     9661 catcaacacc aggatggggg caacagcttt cagtgcgccg acgaacaaag tacctaacag
     9721 accaacagct tccgccgcgg gttttgagat ccatgccaga agaatcccca gaacaaggcc
     9781 gaccaggatt tgttttacca ggctgccatg agccagacgc cggaatagcc ccggtgaacg
     9841 ttgcgtagtc atttttcgat cctttcattg tgttgtcgca catccctggt gcgttctgtc
     9901 gacgcatctt tcaggatgta acaaagtgtt tgcaaggtcg agtataagga aagttacagc
     9961 ttcgggaagc gaaaaatgct ggattttaaa gagaggtcat gttttgcaac ttaatggttg
    10021 catttattta acataaacag agggaaggac tggctactaa ggctgattag ataacatatg
    10081 ggcaaaacta tttggaatat gagaacggtt gcgctccttt aacgtgttat gccatataaa
    10141 gtcactggcg cagggagcgc gcagggggcg gccaatcgcc gccgccccct gcacccccag
    10201 gctctggcga acaaaatcgc cgctgcgcgg ttccctcagc ctatcccttg cggctaccgg
    10261 gtcgggcgca aggtaacgtc cctgtaagat gcgccctcgc cccggcctcc agggaacgct
    10321 tcggcgattt tgacgccacc aacacccgtg cggtcttttt gtaaagagct ataagccgat
    10381 tacataatta atagctcacc tttgtctgga atgagttatc tctaggaatg gcttaatata
    10441 cagcgaatca ttctccccgc tgcttatcat gccgataatt cacccaggcg ttgataataa
    10501 acgtcatcac cagaatgcca aacaccacgc ccagcgagac ggcgattgga atatggtaga
    10561 agtcgacaat cagcatcttg ataccgataa acaccagaat caccgccagg ccatatttga
    10621 gcatcgagaa acgctctgct acgcccgcca gcaggaaata catcgcacgc aggccgagga
    10681 ttgcaaacag gtttgaggtc agcacaatga acgggtcagt ggtcacggcg aagatagccg
    10741 gaatgctatc cacggcaaaa atcacgtcgc tcaattccac cagaatcagt accagcatca
    10801 gcggtgtggc gtacagcaat ccattcttac gcacaaagaa atgctcgttg tcgatggtgt
    10861 cggtcatgcg caaatgaccg cgtagccagc gcaccagcgg cttgtcgcca atgcctgatt
    10921 catcttcatg ggcgagcgcc atcttcacgc cggtaaacag caggaaggca ccgaagatat
    10981 acaggatcca gtcgaactgc gaaatcagcc agctgcctgt gaagatcatg atggtacgca
    11041 gaacaatcgc cccgagcacg ccatacacca gcacgcgacg ttgtaatgcc gccggaacgg
    11101 agaaatagct gaacaacatc agccagacaa agacgttatc gaccgccagc gatttctcaa
    11161 tcagataacc cgtgagaaag gccagggcct gtggatcggc aacggcgcga ccttcggttt
    11221 gcaccagata ccaccagaaa gcggcgttaa acagtaacga cagcgtcacc cagaccagcg
    11281 accaggccgc agcctgtttc atggtcatgg catgtgcccc acgacgcccc tgcaacaaca
    11341 ggtcgatagc cagcataatg gcgacaacaa cagcgaatcc gccccatagc aacggcgtgc
    11401 cgacagtatt catagaagtt ccttacacat aaaaaaacgg ccaacgtcgg aagacgtcag
    11461 ccgctgcttt ttatgcatag acctcgcctt ccggcaaggt ctcacttaca acaaaaaagg
    11521 aatacgtctg cgtattcctt tcgggttgcc cggtgaccgg atgtggtttt tcactcatcg
    11581 taatgacgat cgaccggcaa tgaagttact cccctttgca ggtaacaaag tatgacagac
    11641 cattcagtca gtcaatggtc tgttacatac cttttacact gttttacttt ggcaagtata
    11701 gttttacgct atctgccgga aaaatcaccc cagtttggcg gcggatctcg gtcagcagtt
    11761 tggctgtgat gcgactgacc gccagccctg gatgatccac cagatgctca tccaccaggg
    11821 tagcgaacag ctctgcttcg tagagcatgg tattaatatg ctgcggctgg gtgagatcct
    11881 gcatctggct gccacgcggc acaaagcaga ctttctggca ttcagacagt ttttcaatca
    11941 ccagcgatcc cgcttcgccc tgaatctcgc ttcccaggac agaatcactg acctttgagt
    12001 gctgcaaggt gacgctgaaa tcaccgtaat ccatcaccac cacgccgtgg gcgtcaacgc
    12061 cgcttgccag caaactcgcc gttgcctgca cgcttttcgg ctcaccaaat aacgccaccg
    12121 ccgacgccag gcagtaaaag ccgatatcca taattgaacc gttagagaat gccggattaa
    12181 aggtgttggg attctcaccg tccaggtaac gttgataccg cgaggaatac tggcaatagt
    12241 tgaaaaagac tttacgcagt ttgcctgctt tcggcagcgc ctggcgcaac aaatgaaagt
    12301 tcggcaggca ggcggtttta aatgcctcaa acagcaccac ctgattttcg cgcgcacagg
    12361 caatggcggc atccacttcc gccagattcg acgccagcgg tttttcgcaa atcacatgaa
    12421 ttttatggct aaggaaaagt tgtgtctggg aaaagtgcag ggaattcggg ctggcaatat
    12481 acaccgcgtc aatggcatcg ctttccgcca tcgcttccag cgacgtaaac agatgctcga
    12541 cagaaaaatc attggcgaag tgctgagcct gctcaaggct gcgggaatat acggcggtta
    12601 acttgtattt accgctctca tgggcggcct cgacgaactg gcgagtgatc cagttcgtac
    12661 caatcacagc gaaacgtatc ataacgcgtg gatactccat aaagggattc tgtcgtcaat
    12721 gtagcacgcc tttttcatct tcgacgtgcg cagatacgca ttattctttc gccggacagc
    12781 catgctcact aaacggccca atcatgaccg atggcccgag atatttcggc tggcgatgga
    12841 ggatttttgc gtcaatcacc gcgctgacac gagcgagttt ctcccgcagt tgagtataaa
    12901 aaccgtgctt catattgagg tctggcgcgt taaacccgat agcgtcgatg ccgtactgtt
    12961 ttgccagcca gatggtgcgt tggttatgaa attcttgcga gataatggtg atatggtttt
    13021 ccccaaacac ctttttggca cgcactaccg aatccagcgt tgagaatccg gcatagtcgc
    13081 aaaagatcac tttcgccggt acgcctttag cgatcaaagc ctgctgcata cctgatgctt
    13141 catcgtaatt tttacgtccg ttatcgccac tcaccagtag ccatttcact tttccggcgt
    13201 ggtacaacgc tgctgcggta tcaatacgtc gggtaaagta gcgattaccc ggcctcgccc
    13261 ccagcaataa acccacgtta cgcgccggga cggcgttgac atcgccccag gtcagctgct
    13321 tgctggcgtt gaccataacg cgatcggcga taaaaatggt cgctcctgcc acaagtaaca
    13381 gtaagcaggc aatctttaga gtccggcgag aaaaacaaat acggagaaga aggcgggcaa
    13441 atgcgcgcag catggtcgtt atccttaacg aaagggggga ggtcgatttt aggcgctaaa
    13501 gaaagaaaag ataacgcctg acatcatttt cagattttgt cctgcccaag cgacaactgc
    13561 gttagccaca gccgggtgtc gaactcaagc tggtggtact gcggctccat atggcagcac
    13621 aattgataga acgctttgtt atgctctttc tccttcaggt gcgccagctc gtgcaccacg
    13681 atcatgcgca aaaacggttc cggcgcgttg cgaaacacgg tagcgacgcg gatctctgct
    13741 tttgctttca gcttgctgcc ctgcacacga gaaacagcgg tatgtaaccc gagcgcgttt
    13801 tttagcacat ggatcttatt gtcatacatc actttattga tcggcggggc attacgcaga
    13861 aactgatttt tcagatcctg agtatattgc cagagagctt tatcggtggc gtagtcgtgt
    13921 gttcccggat agcgttttgc cagcacatcg cccagacgct gttcgttaat cagtgttcgc
    13981 acctgcgaaa gcagctgctc gggataaccc tgaagataag taagattgct cataaccgcc
    14041 cacgaaaaag agaaaaaggg tatactcacg cacccttttc aggggcaacg tcgaaatttt
    14101 atcattcagg agggccgatg agccacttag acaacggttt ccgttcactg acactacaac
    14161 gttttccggc gacggatgac gttaacccgc tacaggcgtg ggaagcggca gatgaatatt
    14221 tgctgcaaca gttggacgac acagaaatcc tcggcccggt gttgatcctg aatgatgcct
    14281 ttggtgcgtt aagctgcgcg ctggcggaac ataagccgta cagcattggc gactcataca
    14341 tcagtgaact ggcgacgcgc gagaatttac gcctaaacgg gattgatgag tcgagcgtga
    14401 agtttctcga cagcaccgcc gactacccgc aacagccggg tgtggtgctg atcaaagtgc
    14461 cgaaaacact ggcattgctg gaacagcaac tgcgtgcgct gcgcaaagtg gtcacgtcgg
    14521 atacacgtat tattgccggt gccaaagccc gtgacattca cacttccacg ctggaactgt
    14581 tcgaaaaagt gctcggcccg accaccacca cgctggcatg gaaaaaagcg cgcctgatta
    14641 attgcacgtt caatgaaccg ccgctggccg atgcgccgca gaccgttagc tggaagctgg
    14701 aaggtactga ctggactatc cataaccatg cgaatgtctt ctcccgcacc gggcttgata
    14761 tcggcgcgcg cttctttatg caacatctgc cagagaatct cgaaggggag attgtcgatc
    14821 tcggttgcgg taatggcgtt attggtctga cgctgcttga taaaaacccg caggcgaaag
    14881 tggtgtttgt cgatgaatcg ccgatggcgg ttgcttccag ccgtttgaac gttgaaacca
    14941 acatgccaga ggcgttggat cgcagcgagt ttatgatcaa caacgcgctc tccggcgtgg
    15001 agcctttccg ctttaatgct gtgctctgca accctccgtt tcaccaacaa catgcgctga
    15061 ccgataacgt cgcctgggag atgttccact acgcccgccg ctgcctgaaa atcaacggcg
    15121 agctgtatat cgttgccaac cgccatctgg attacttcca taaactgaag aaaattttcg
    15181 gcaactgcac cactattgcg acgaataata aatttgtggt gctgaaagcg gtgaagctgg
    15241 gacgtcgtcg gtaagattgg gagttacctg ccggatgcgg cgtaaacgcc ttatccggcc
    15301 tacgttcagc acgttacccc caggccggat aagacgcggc aagcgtcgca tccggcaatg
    15361 tctgctcagt tctggaatcg cgcttccata aaacaaaaaa tccctgtcac catcctctac
    15421 attctcttgt ttagcgtttt tctacgttta ttcttccgtc acacagataa attcatccgt
    15481 tgccaatctg tcaacggatg ttatcatatt gccaatttgt caacggacgt gatgaagaat
    15541 gcacctgata actcaaaaag cattgaaaga tgctgcggaa aaatacccgc aacataaaac
    15601 ggagttggtg gctctgggga acacgattgc taagggatat ttcaaaaaac ctgagtcatt
    15661 aaaagcagta ttcccatctc tggataactt caaatatctg gataagcatt atgttttcaa
    15721 tgttgggggc aatgaattac gtgttgtagc aatggtcttt tttgaatcgc aaaagtgcta
    15781 catacgtgaa gttatgacgc ataaagaata cgatttcttt accgctgttc atcgtactaa
    15841 ggggaaaaaa tgattgcgat tgccgacatc ttgcaagcag gagaaaagct aactgctgtg
    15901 gcaccttttc tggcgggtat tcagaacgag gaacaataca cccaggcgct ggaactggta
    15961 gatcatctgc tgctcaacga tcctgaaaac cccttgctgg atctggtgtg tgccaaaata
    16021 accgcgtggg aagaatcagc gcccgaattt gcggaattta atgccatggc tcaagccatg
    16081 cctggcggta tagccgtgat tcgtaccctt atggatcaat atggtttaac cctttccgat
    16141 ctgccggaaa ttggcagtaa atctatggtg tcacgcgttt tgagcgggaa gaggaaatta
    16201 acgctggaac acgctaaaaa attggcaacg cgattcggca tttctcccgc cttgtttatt
    16261 gattaacgcg acgtgccgaa tgcgttgcat ccggcgcttc aaataacgat ttaaatctcc
    16321 agcgccagcc gtgttccctg ggcaattgcc cgtcgtgcgt ccagctccat agccacatcg
    16381 cagccgccaa ttaaatgcac agttttcccg ctatcaatca gcggttgcgc cagcgcgcgg
    16441 tttggctctt gccctgcgca gatcaccaca ttgtccactg ctaatacctg cgtttcgccg
    16501 ttgatcacca catgcagccc gtcatcgtca atcttctgat aacttacgcc tgggatcatt
    16561 ttcacacccc gcgagagcag ggtggtgcga tggatccagc cggtggtttt gcctaacccc
    16621 tgtcccggtt tgctggcttt gcgctggagc atcacaattt gccgtgggct acgcgggatc
    16681 tgcattccct gcgggcttaa gccaccagcc tgttgtaggc tgctgtcgat cccccactca
    16741 ttacagaacc tggcgatatt ctggctggtg gattcgcccg gctgacttaa atacatcgcc
    16801 gtatcaaaac caatcccgcc acaaccgatg atggcaacct tgttgcctac cggcgctttg
    16861 tcgcgcagta catcgagata actcaatacc ttcggatgat cgatcccgtc gataggcgga
    16921 acacgcggca cgatcccact ggcgaggatc gtttcatcga acgcctgtaa ctgatccgcc
    16981 gtcacggtgt gattgagttt cagcgtcacg cccgtcactt cgatcatccg gcggtagtag
    17041 cgcagcgttt cgtaaaactc ctctttgccg gggatctgtt tggcgatatt aaactgcccg
    17101 ccgatctcgc tgtgcgcatc aaacaatgtt acctgatgcc cacgcgccgc cgcgttaatg
    17161 gcaaacgcca gcccagcagg tccagcgccg accaccgcca gatttttttt ctgcacggcg
    17221 ggaaggattg gcattttggt ttcgtggcag gcgcgaggat tcaccaggca cgaggtgact
    17281 ttgccaacga agatctgatc gaggcagccc tggttgcagc caatacaagt gttgatctca
    17341 tcggctcgcc ctgattgcgc ttttgacagc agttccgcat ccgcgagaaa cggtcgcgcc
    17401 atcgatacta tatcggcatc gccgcgcgag agaatatcgt cggcaacctg cggatcgtta
    17461 atccggttgg tggttaccag cggcagcgag acgtggcctt tcagtttgcg cgtgacccag
    17521 ctaaatgcgc cgcgcggcac gggcgtggcg atggtcggaa tccgcgcttc gtgccagcct
    17581 attccggtgt tgataatggt cgcgcccgcc gcttcaatgg cctgcgccag ttctaccgtt
    17641 tcggcaaaag tgccgccgcc ttcgactagg tcgagcatcg acaaccggta aataataatg
    17701 aagtcgttgc cgacgcgttc gcgcaccgta cgcactactt ctacggcaaa tcgcatccgg
    17761 ttgcggtaat cgccgcccca ctggtcacta cgctgattgg tgcgcagcgt cagaaattcg
    17821 ttgatcaaat acccttcaga gcccatcact tcgacgccat cgtatccggc ctcccgcgcc
    17881 agttgcgcgc agcgggcgaa atcgtcgatc agttgcagga tctcttcatg ggtgagttcg
    17941 tgaggaacga aacggttgat gggggcctgc aatgcggacg gtgcgaccag atgcggttgg
    18001 tagctgtagc gcccggtatg caaaatttgc agggcgattt tgccgccttc ctgatgtacg
    18061 gcttcggtaa tggtgcgatg gtgtgggatc tggctggcgt cgttgagcat tgcgccacct
    18121 tccatgccaa cgcctgttaa atctggtgca atgccgccgc tgacaatcag cgccacgccg
    18181 tgacgggcgc gttcggcata aaacgcagcc agccgttcag caccgtccgg gtattcctcc
    18241 agcccggtgt gcattgaacc catcaacacg cggtttttta acgtggtaaa acctaaatcc
    18301 agcggggcga acagcgacgg gtagctcata aaattgtcca gtatgtaaaa taattgttat
    18361 gtagtcggat gagttctaat ttagccgtcg atggagaaaa gggaaaaggg gagtgcgggg
    18421 tttgtgatgg gattcaaaaa gaatacgccc ggtggtaacg ccggatgcga cgcttgccgt
    18481 gttttatccg gcttggggga ttgcacatgt agggcggata aggcgttcac gccgcatccg
    18541 ccagccgttg caaattactg cttacggaaa aaatcgttat acaacatata caaatgagcg
    18601 gcactccagg agaaatttgg tgcgccttgc tgcgcgcctg tcagcgggtt gtaattctcc
    18661 tgaattgggc catcggcggt taatcctttg gcgtgcctga agaacgtatc cgccagcttc
    18721 agggcatcat cacgataacc gtaacgctcc atccctttca gaccaaacca gaactgatcc
    18781 acccatacgc gcccgcgcca gtagatatca gcgccaaaag ccggattggt taacgccgcc
    18841 gttcccagcg gaacaaatgt gttgaactct ttaggatcga gcatcacctt caccaccgcg
    18901 tcggcattgg cctgcgttgc cgcaccgtta aacagcggcg accagccttc cggcccttta
    18961 ccgcgctcaa caatcggttt gcccgcgcag ccgttcgcca gtggtttatc ttcaatacgt
    19021 acgtcatagt agaactgggt agtcgggtcg aacatacagg tgttgatgta gtccgcgagc
    19081 tgctgtgcca actggcgata gcgtttggcc tcttctggct taccgaggat ggttgccatc
    19141 tccgccagat aatggttatc gctgtacata tagctggcct gatccaccga ctcctgcaat
    19201 agcgagtagc ccagcaacgt tccgtcctga ctgcggtttt cggcgaattt caccgtccag
    19261 tcgctacgtt tgccgccatt agcgacatat ttatccaact gttctttgtc gataaaacca
    19321 aagacggcgg catcatcacg ccccgattcc cacgacgcag cgacctgtgc cggaatttcc
    19381 agactgtcgt actggccttt ctccaccacg cgggcgtagt tgttcagccc agactgcgtc
    19441 tcttctttat cgcctttttt caccgtaaac agcatctcgc cgctctcggt gttgtgggct
    19501 ttgtcgcggg tcgcgccata ttccggcacc ccgttgccgt tatgatcgcg gttacgtaac
    19561 caccagtcgt gataggccac cagtttcggg tacatctctg ccaaccaggc tttatcctgg
    19621 gtgacgttgt atacttccat caccgaccag gcggcaaggc tgggtttggt attacgttcg
    19681 ttccagttgc cgccatcgcc accacgctcg gggctaaggt tccacgctat caggtcgggg
    19741 acaaagccca catcctgcgg acgcacgctg tcgccaggct ggatctgcca ggagaagacc
    19801 gcgcggatat tctctttggc gatatccgga ttgaaatgcg ccatcgcaaa cgcctgcttc
    19861 caggtatccc acggccaggt ctgattgccg gagaaccagc gtccggtcac cgacggcgtg
    19921 acggtgttat aattcaccgc accgcccggt gagcgccagt taccgttgag cgtttcgatc
    19981 gctttcaccg cgacgcgcgt ctgttccggc gtcgcatccg gattggttaa ccctttcttc
    20041 aggtattctt cccagcgttg ctgcgaggcg gtgagataaa acgccggacg cgccagaata
    20101 tcgcggatct gcatttgctc tttgctaact tcctgagcgg tcagcagatg ggaataggtg
    20161 gtgtagagcg tggtcgaacc gttgatatgc gccttactgg taaagcgatt accgttgatt
    20221 tcagtctgca ccggcaggga tttatgcacc tgatattccg attcgccgga ggtcagcaga
    20281 tcccaggtgg cgcgcacttt gccaaaggtg actttcaggc catcacgggt ggcgctgatt
    20341 ttgcgctgat agtcagggta ttcgccagca atggttttat cggaaagcgg tttcccttct
    20401 ttcgcttcca gtttttccag cagttcgcca tcccacacca gatccagcgg tttattgctg
    20461 atgattttag tttccagtag tgacgtgcgc ggcgtggcga agcgcagagt catttcgacc
    20521 tgcacatctt ttgctgtcag tttttgcacc agcgcgccgg gaatactgta tgcctccagc
    20581 gtgaagttga ctttcttgcc gtcttgccag acagtcaggc ggtcgaaatt gctggccata
    20641 aagttgatgt attcttccgt cagcagcgca acgcccggaa agccgcccat ggtgttcggg
    20701 ccgtctggca acagatgacc atgccaggct ccgagatcga aaaacggatt aaaacgctgg
    20761 tgatcgtcgt aatcgtaatc cttcatgtac tgcggcgcac cagtacggtt aatcacgttt
    20821 ttataattgt cggcgttagc ggcatgagcg gaaaaactta tcagcagagc gcaggttact
    20881 ggcgttaaaa tagttttgat tttcatgaga ttacagtcct tattaccagt agaaatattg
    20941 catcaggaag atttggtcgc tgggatcgtt agaggcattt ttcatgtaga agcggctatc
    21001 aaaagccgtg gcgaaacgtc cgccgccata ttcgtaccag ataccaaact gcatgaaaga
    21061 ggtggtttca tcgtcagcgt ggtcagggcg atgtttggcg taggtgtaag cggtggagag
    21121 ataaacgcct tttaacgctt catacatggc gctcaccatc atcccgcttt cacttcccgg
    21181 tacgtccagc ccgtcggcac gtccggtatg gtgccaggcg cgagcggcaa agttaggggt
    21241 aaacaggccc gtgagataga gttgtcctgt gccgtcggta atttccagac cattcatcca
    21301 ggtgatgcct tcggcaaggt catactggat gtaaccgttc atccattgcc ggataggtgt
    21361 atttgtcgtc gtagcgatcg tacttgccaa aatgtagcca ggctttgctt tcgtcatgat
    21421 gtccggcagg tgtggcggtg acgctataac gaaagttgcc gcttaagttc tgtaccttca
    21481 gcgcggtcat aatgtcgcgg gtattgggaa tgacgtagcc caaatcgggt gtgaagtcgc
    21541 cccaccattg caggtcatcc agcgaggaat cctgacgcag actgagcatg atttcggtgc
    21601 catcattggt acgataaccg ccatagaaac ggttaatccc accttcgaaa ccaccccagc
    21661 tatggtcagg cacctaggca ttaccgtcgt ggtcggcctg cacggtccag ccttcgccgt
    21721 aaatcaggcc gaaccaggag ccgtacttca tctccaggcc accttcaata taggtgccgt
    21781 cgctgtattt atgtttattc tcaccctcta aatccatata tccaccaagc cctaattcgc
    21841 cgtaaaaacg aaatgaagga gaagatgtcg aggtaattgc gggtgttttt gtgtcgtcat
    21901 gttctgccgg acgtgcttct tctcctgccg cagaaaaggc gtagcaaaac ggcagagcaa
    21961 gtaatgctct gcatggtgca gtgattaatt tcattgtgtt aatccatgag ctaaagaaaa
    22021 agtacagcct tttatcgttg taattataaa aatataaagg cagaaactat tttccctctc
    22081 ctcatccagg aagagggaaa atgcctgctc attttttatt agtctgctta atcaacattg
    22141 ctggctggcg tcgcttcaat atggcgctct tcagccgtta atccctttat atatttgctg
    22201 tatttccacc acgcaaagcc gaggaagata acaataccgc cgacgttata aaaaatgatg
    22261 gtcagaatat tcgcgccagt cgggaatgtc gaagcgacaa accctacggc aaatatcgca
    22321 atcagcattg aaacaacgat aataccggtg cggcgggagc ccatacggaa atcgcgtggc
    22381 aggtgatcta atttggcgcg taaattcagg taagccagca tgatgaataa cggcggaagc
    22441 atggacgctg cggcggtcat attaataatg gtattcatca gatcctgcac ggtattggaa
    22501 ccgagcatcg ggataatcat cagcgggatg acgatcagaa actggatcca tgctgcgcgc
    22561 gccggaacgc cgttttcgtt aagttcgacg gttttcttac caaagatgcc ttccgggatt
    22621 tcggagaaga aaattttcac cggcgttgcg gtccacatca gcagggaacc gaacattgcg
    22681 gtaaaggaca ccagaccgac aaagcgattc atcaacgctt ccggcagacc aaaatacgcc
    22741 gccatgccgt ggaatacctg caccgatccg ccggtaaatt tcaactcttt gctgctgacg
    22801 aagacgttaa tcagcaccga ggagacggaa tacagtacgc cgataaaaat cccggcgagg
    22861 atgatcactt taacgaacga tttcgaaccg cctttgacgt cgttaacgta caccgcgacg
    22921 gactccgcgc cacctgcggc cataaagatc caggtggtaa cgccgaggaa cgcccagttg
    22981 aagttcggga tcatcgcgtc aacggtgatc ggatcggcag gctgtacgcc gccaaccagt
    23041 gccgtacctg ccagtaaaat gtaggagagc gttaacagca gcatcagcgt tgaagtgacg
    23101 gaggtaattg gccccagcat tttcgccccg ttggtggaaa cccaggtgga gaaggcgaac
    23161 agcaccatac tgataacggt ggtggcaacc ggcgtcataa tatattcgta gccgaggaag
    23221 gcatacgaag cataagcaat aacgcgcggc aacagcgagg tgaaaaagaa cagatttacg
    23281 aaccagtaag tgtaggcagt aataaatgcc caacgtccgc ccagcgaact ttttacccac
    23341 gcgtagacac cggcttctga gtttttattt aacgaaacaa attctgcgat gatcagacag
    23401 aagggaataa aataaaaaat cgtcgcgagg aaaaacatcg gtgccgaggc cagtccaagc
    23461 tcaatattat tattgataac gttgttaaag ctgtaaacgg cggcaaaagt cagcgagagc
    23521 aagccgaatt tgccgattgt attacgtttg gtatcagaca taacacatct ccgaaaatgt
    23581 gtagggtagc ggtggcggca aactcctttt gccgccgtag ttgttatttg ttatggaaat
    23641 aaccatcttc gatggtgact ttgagaacca cttttttgac cgcgttattg cagataaacc
    23701 gatacgcttc atggatatcg caaataacga tttgcccttc gtggacctct acggtttctc
    23761 cgcagccttt taaatattca cggtcagttt catcacgata atattccact acctgtaatg
    23821 tctctttcgg cgcatattca attttttgct gcccttgcag gtagtaatgc acttcaaaat
    23881 agcgtcgatg cccggtaaat agcgcatcgg tcgcggagtc attttccaca cggtaggtca
    23941 atgaatcacc gatggagtgg gcgacgccag gctgaatgtt gtcgatattt tcaatcgctt
    24001 caacgcagcg ttgccacttc ttgccagagg cgtaaatctg gcggaactgt tctaagttat
    24061 cgatgatcct cattgcttat tctcgctgtg caaattcgtg gaaaagaacc ctgcgccgaa
    24121 ctcatacgac gccaggcttt gcgcggtagc ttctccgcca gaaaccggca gcaacgtaaa
    24181 gccgtagctg aagtcacgga accagacgcg ccaggagtcc agcacctcgc tgccccagga
    24241 gttggagccg aggccaagta gctggtggtc gaggttcagg gtgatgtcat cactgcgctg
    24301 cagctcgtta cagtgctggg cagcgtggat gttttcctgg gtatagtgcc aggcgctgaa
    24361 gttaattggg cgctgcggaa ccaccagcag accgttgccg tggcggttag tcagtgccgt
    24421 ccagcggaca tgctgacggt tgccgttgtt ctgcgggaag ggatagttct cgaacatggc
    24481 atcgacggtg ctgcgccaga tatcgatgat gttagcctgc tggctgtcgg cgtagttttc
    24541 gcccggtcca cgaccgtaat acgccacctg atcgtattcg ccgttaatcc ccatggtgaa
    24601 accgatgcac ggaatgatgt gcggatagtc gccgtaacgc tcgccggaaa gcgccacgtt
    24661 aacctggcca tcggcagcga tgcgccagat gtaggtacag cgcatcccga agtcaaacac
    24721 cggcggtgca ataactgtgc ggctgatgat caacacttca ccatcgctct gttctacggc
    24781 aaagtcgcgc agatgttcct gcatgatctg caaatgattc ggctgccaca acccttcgta
    24841 ctcctgcttg tggttgtcga tcatcggctt gaagaagttg atctttggct cgcgggtcag
    24901 cagcgactcg ccatttacct gccaggatgt cggtttgcca ctcatttttg agaaggtgat
    24961 cgcgaagttg tagccgcgaa cggtgcagct caaacgatcg tcttccagcg tcagcggacg
    25021 cgcattattt ggtgcgaaag gcactggctg cgcggtgttt tccttcagcg ggaactgata
    25081 agtggcgata gaatgcccgg cttcgctgta gcgggtgcgg gaatctttgg tcaccgtaat
    25141 gttgaggaac gcttcgcggg cgtccagctg cggcagcgtg atctgcaagg gggcttcgct
    25201 gttcggcgca acgtcgcgca gtttaatctg ctgcgtcgcg agcgtttcac cttcggcgcg
    25261 cacctctgcg tgcagggtgt agtcatcaag cgtggtaaac cacagtttat tttcgacttt
    25321 caactcgccg cgagtcagcg cgtggatttt taccggcgcg ataacctgtt tgtactcttt
    25381 caggcccgat cccggcgtct gatcggaata gatcaaacca tcaagacaga agttatagtt
    25441 gttgggatag tcgccgtagt cgccgccgaa tttataccag acattgccgt ggtcgtcctg
    25501 tgcctggatc ccgtggtcgc accactccca gacataatga ccctgaatgc aatcgtgctt
    25561 atagaagacg ttctggtact ccgtcagccc gcccggtccg ttccccatcg catgagcata
    25621 ttcacagatg atgcgcggct tcggatgcgg gtattcacca aactcattca tcagcggcac
    25681 gcgggtgtac atggtggaaa taatatcgac cacttcagca tcgcgatctt cttcgtaatg
    25741 aaccagtcgc gtgtcatcca gcgctttcgc cgcatggtac atcgcgcgga tgttacagcc
    25801 atagccggat tcattgccca gcgaccagat gatgatcgac ggatggtttt tctgtgcgtg
    25861 gatatggcga acaatgcgct cgacgtagac tttttcccac tgcggatcgt cggtaatacg
    25921 gctgatatcg ccgacattag caaagccgtg cgattcgacg tcggtttccg ccatcacaaa
    25981 caggccgtag atatcacaca gttcgtaaaa acgcggatcg ttcgggtagt gagcggtacg
    26041 cacggagttg atattgtgct gcttcatcaa ctggagatct ttctcgacgc gatccattcc
    26101 aacggcgcgg cctttgcgat gatcgttgtc gtgacggttg acgccgtgca gcatcacata
    26161 acggttattg atccagaaca gaccgtcgcg cactttgata tcacggaagc caacgcgttg
    26221 tggcaccact tccagaacgt tgccgttggc gtctttcagc gtcatgacca gatggtaaag
    26281 ataaggggat tctgctgacc attgctgcgg ctgttcgaca gtaaaagcaa agctggcgct
    26341 ggtcagtttt tcaattgcca aatgatcaat ggcgctgctg tgcaccacgc gttcgccatc
    26401 aaacagggta tattccagcg tcgtgacgac aggggaggcg gcgagatttt ccagcaccac
    26461 ttcgcaggaa agcgtggcat cgcaataggc ttcgtcaaag tcggtacgca cagtgaaatc
    26521 gttaatatgc gttaggtgtt ttccgaccag ataaacatcg cggaagatcc ccgccgacca
    26581 ccacatatcc tggtcttcca cgtaggtaga gtccgcccac tgcatcacgc gcacacacaa
    26641 caggttgtcg ccggttttaa ccatcgcgct gatgtcaaac tctgcggtca ggcgactgcc
    26701 cttgctgaaa cccacatact gaccgttaac gtagacttca aaataggttt cgacgccgtc
    26761 aaatttaatc agcgtctgtt taccctgcca gccgtcgctg agggtgaaaa tacgttgata
    26821 ggcaccggtt gggttatcgc tggggacaaa cggcacatcg atggggaacg gaaaaccttc
    26881 gtcggtatat tgcagtttgc cgtgaccttc catttgccac atggcgggga cggtaatatg
    26941 cccccagtca gccattaact cagaggtgaa ggcttctggt acttgcagcg gatggtcaaa
    27001 aaagtggaaa ttccactgac tgcttaaggg cagaaacagg ctgctggttt cgcgggcaaa
    27061 ggtacgcgct tgcgcaacag aatcatatga aaaaaagtac gcacgcggcg caagtcggtt
    27121 ttcgtgggtg agctgaatgt tttcccagcg attcataggg cctcccggta gagagaaatc
    27181 ggaaccattc atggtgatgg tagtttgtgt aattgctggg ggtttagtaa aattttttac
    27241 taaaaagcag caggtaaatg catttacttt aactggatca cgaaaataaa cggcagcact
    27301 gtccggcaaa aatgattgcc gaacagtgtg taaggggatt taacgggtcg tgccgcgcag
    27361 ttttaatttg ctgggaacga agactagcag cggcagcgcg cgaccatcgc gggctttttc
    27421 atacaccagg ttaacgccct gacttcccat catctcggaa tggatgcgca cggtggagag
    27481 cggcggaaag gtaaatcgcg cggtggggat atcgttaacg ctgataagcg aaatatcttg
    27541 tgggatgttc aggccacgtt catgaattgc ccgcagtacg ccgatagcaa tggaatcgga
    27601 agcaacaaac agtgccttcg gatagtcttc ccgcgccagc atttgttttg ccagttcata
    27661 acccgacgaa ctggaaaaac cgccgcgcca gatatcctct tcgcgtacca cttgtttcag
    27721 tcggccatat tccgcaaagg cgacctcacg aatatccgcc ttgccaggct catcttcacc
    27781 gccaataaaa ccaatacgat taacgccctg gttgatatag aagtcgatga tttctttact
    27841 gatgcgtgcc agatcgatat ccaccgcatc gtaaccgctg ccgggttcgt gaaagtcgat
    27901 aaaacagata ttgtcggtca acgcgctggc agcggcgcgc agggcgggcg tgggtttgcc
    27961 gacaattaaa ataccggtga cgtttttaat gtctggtaat ccgttgtgtt cataacagtt
    28021 ggtcagttcg atcgccagct tttcgcactg agtttcaatg ccgtggcgga tcgccagata
    28081 gtaaggatcg ttgatctcca gctcctgctg gtagctgtag atagccagaa tatggtgttg
    28141 gttgactgcg cctgtctgta gtttacgggc gctactggtc ttgtactcca gcttttcggc
    28201 gatctcgaga atgcgatgtt tcgtctcttc tttcacattc aatgtcggat cgtcatttaa
    28261 gaccctggat actgtcgcca gggatacgcc agcttcgatt gcgatgtctt ttaatgttgc
    28321 catgtttacc ttttttactt tccctgatgt gcgcccccat tgtagaggaa gtgcttatcg
    28381 ggtcacctgt tttcagtaaa taaagcgcgt ttctttgacg ctgtaacggt aacacagcgg
    28441 cttaggaatg tgatcacgcc tgcattctcc atttttagcc tacaggattg agaaaaactt
    28501 ttctatagtt tagtaaaatt ttactcgaag ggaggaagca atggaaaccg tggcttacgc
    28561 tgattttgca cgtctggaaa tgcgcgtcgg aaagattgtg gaagtgaaac gccatgaaaa
    28621 cgccgacaag ctgtacatcg tacaggttga tgtggggaaa aaacactgca aaccgtgacc
    28681 agcctggtgc cgtactacag cgaagaggag ctgatgggga aaacggtggt ggtcttatgc
    28741 aatctgcaaa aagcgaaaat gcgtggtgaa acgtcggaat gcatgctgtt gtgtgcggaa
    28801 accgatgacg gcagtgaaag cgtgttgtta acgccggagc ggatgatgcc tgcgggcgta
    28861 cgcgtcgtat aaaaagatcg gatggcgacg tcgtatcgcc atccgatttg atattacgct
    28921 tcttcgacac ttactcgcat ggccgccagc gccttacgcg ccgctttgat caccagttca
    28981 cactgttcaa tggtcagtgt cagtggcggt tcaatgcgga tcgttttggc gttattgagc
    29041 gttccggcca ccagtacgcg ctggcggaac atctcgctgg caaagttata gccgatttcg
    29101 ttatcaacaa actcaatcgc catcaacatc cctttaccac gcgcttcctg taccagatcg
    29161 ggatattccc gcgccagttg acggaaaccg tccagcaaca tatcgccttt ttgctcagcc
    29221 tgagccggta agttctgctc cagcaacaca ttgatggtcg ccagcgccgc cgcacaggcc
    29281 agcgggttgc cgccaaaggt ggtggtatgc aggaatgggt tgtcgaacag aactgaaaac
    29341 acctcttcag tggcgatggt cgcgccaatc ggcatcacgc cgccgccgag ggcttttgcc
    29401 agacacagaa tgtccggctg aacgttctca tgttcacagg caaacatctt gcccgtgcgc
    29461 cccatgcccg tttgtacttc atcgaggatc atcagtgcgc cgaactcatc gcacagctta
    29521 cgcacggcgg tgagatagcc cggcggcggc agaattacgc caccttcgcc ctgaatcggt
    29581 tcgaggatca ccgcagccac atcatcaccg gtttttttgc actcattaag agccgtgcgc
    29641 atggcttcga tattgccaaa cggcacatga cggaaccctg gcagcagtgg cataaacggt
    29701 ttgcggaagg tcgatttcgc cgtggccgac agcgcgccaa gtgatttacc gtggaacacg
    29761 ccgctggtgg caataaaagt aaatttgccg cgcggcgact gataagcctt cgccagcttc
    29821 agcgccgctt caacggactc ggtgccgcta ttacagaaga agctgtattt cagtttaccg
    29881 ggcgttagcg cagcaagggt tttcgccagc atagcccgta acggatcgag tagctcctgg
    29941 ctgtgcagtg gttgtttcgc aagttgattc tgtacggcgg aaaccacaac tggattacgg
    30001 tgccccacgt tgaaaattcc aaaacctccc aggcagtcga taaactcctg tccctgggtg
    30061 tcgacaagcg tatttaaacc tcccgcttgc cactctacgg ctccgtaatc cccgccggcg
    30121 gtaacagatt tgcgatactc taaaaacccc ggattgacat gctctttgaa gtattcaatc
    30181 acctctcggt taagtgcttt catctcctca tgatccagcg ttcgcttctc aatgagattc
    30241 agggcgtggg cgctgcacgc taaagccgat gcgctcgaag gtaacctgtt caaaatatgc
    30301 tccggaggct cgcgtatcac atgataccga tttaagtatt gcagggattg cgccactccg
    30361 gctgcgatcg cgaaaatcgg gataaacaca aatgcggtta tcatttgtgc aatatttaat
    30421 gctatccgtt aacatttgtt gttattttgc acggtttttg tcgggtatgg atttgatgat
    30481 tgctgcgcaa atgcacgttt tcagggcaat aattgcctga tttttgggca agataattaa
    30541 cgttttcgta tagtaacaga gtgaaagtat tttaaatcaa agaattagat ttgtgcttta
    30601 atcgcaaatt gcgatctaaa tcaaattaat cggttaaaga taaccgcagc ggggccgaca
    30661 taaactctga caagaagtta acaaccatat aacctgcaca ggacgcgaac atgtcttctc
    30721 atccgtatgt cacccagcaa aataccccgc tggcggacga taccactctg atgtccacta
    30781 ccgatctgca aagctatatc actcatgcta atgacacttt tgtgcaggtg agcggcttta
    30841 ccttgcaaga gttacaaggg cagccgcaca acatggtgcg tcacccggat atgccaaaag
    30901 cggcgtttgc ggatatgtgg ttcaccctga aaaaagggga gccctggagc ggcatcgtga
    30961 aaaatcgccg caaaaatggc gaccattatt gggtgcgggc caatgcggta ccgatggtgc
    31021 gcgagggaaa aatcagtggc tatatgtcga ttcgtacccg ggcgacggat gaagagattg
    31081 cggcggtgga gccgctgtac aaagcgttga actccggacg taccagtaag cgtattcata
    31141 aaggcctggt ggtgcgtaaa ggctggctgg gtaaactgcc ttcattaccg cttcgctggc
    31201 ggacgcgtgg agtgatgacc ctgatgttta tcttgctggc ggccatgctt tggtttgttg
    31261 ctgccccggt ggtgacgtat ttcctctgtg tgttagtggt attgttggca agcgcttgtt
    31321 ttgaatggca gattgtgcgc ccgatagaaa atgtcgcccg tcaggcactg aaggtggcga
    31381 ccggagagcg taatagtgtt gagcacctga atcgcagcga tgagctgggg ctgacattac
    31441 gcgcggtagg gcagcttggc ctgatgtgcc gttggctaat taacgatgtc tcaagccagg
    31501 tgtccagtgt cagaaatggc agtgagacgc tggcgaaagg caccgatgaa ctgaacgaac
    31561 atacccagca gacagttgat aacgttcagc aaacggtggc gaccatgaac caaatggcgg
    31621 cgtcggtgaa acagaactct gccacggcgt cggctgccga taaactttct atcaccgcca
    31681 gtaatgcggc agtgcagggt ggggaggcga tgaccacggt gatcaagaca atggacgata
    31741 tcgccgacag tacctagcgc attggcacca ttacttcgct gattaacgat attgcgtttc
    31801 agaccaatat tctggccctg aatgcggcgg tggaagcggc gcgtgccggc gaacagggca
    31861 aaggttttgc agtggtggca ggggaagtgc gtcatttagc cagccgcagc gctaatgctg
    31921 ccaacgatat tcgcaagctg attgatgcca gtgctgataa ggtgcaatcc ggttcgcagc
    31981 aggtacacgc cgccggacgg acgatggaag atattgtggc acaggtgaaa aacgtcaccc
    32041 agttgatcgc ccagattagc cattcaacgc tggaacaggc cgatgggctt tccagcctga
    32101 cccgtgcagt ggatgagctt aacctgatca cccagaaaaa tgccgagctg gtggaagaga
    32161 gtgcgcaggt gtcggcgatg gtgaaacacc gcgccagccg actggaagac gcggtgacgg
    32221 tgctgcatta atcggtatgc cggatccggc ggttggagca caatgcctga tgcgatgctg
    32281 gcgcatctta tcaggcctgc ggggtgtgta gcgggtgtag gccggataag gcgcgtcaag
    32341 cgtcgcatcc ggcaattgca ccgcgccact ggcggatgcg gcgattaatc cagttgcgta
    32401 atatcaaaag cggcgcggtc gatcaccgca atgatctttt ttatttgtgc atcactgata
    32461 tcgctctggt tgacgcgtaa atccagcacc gctttaaaat tatccagcgc ccgcttcatt
    32521 tgcgggttct ggcgcagcgc cgcgccaacg caacgcgctt tgatgcgttc ttcaatcatc
    32581 tccacttgtt cgcggttttc ttccagccac tgcgcgcctt gttcggtcag cgcaatctgc
    32641 ttcttaccac cttcctcttc gcggatggta atcagcgact gctcctgcag aaaatccagc
    32701 gtcgggtaga tgacgcccgg gcttggggtg taattcccct gggttagatt ctcaatcgct
    32761 ttaatcaatt cgtaaccgtg gctgtcatcg cgcgagagaa tatccagaat caccagacgc
    32821 aattcaccgt gaccaaagaa acgttgccga cgaccgccgc cgcgaccgtg gcgaccaccg
    32881 caacattgac cgtgttcatg ctggtgaccg tgtccgcagt gctcgtgttc tgacttctca
    32941 cctttgcagc agccctcatg gcgtggctgg ccttcatgtt tacaacaccc ttcgtgatga
    33001 tggctcatat cagcctcctt ttgcttgatt agatatatct aatttatatc tgatgtgagc
    33061 attactgcaa gtacaaaaat gatttttaaa cgtggaagca cctcacatct aggccggata
    33121 agacgttcac gccgcatccg gcagcattgc tatggcactg aatgccttca catacttcag
    33181 acatatcagt tctacaaatc gcttgcattt atcatgatta acaatcatta tcatttgcga
    33241 attttattta gatatatctg attaccatca cgaaggcgat aacaatgaat aacacccccc
    33301 gctacccgca gcgcgttcgc aatgatctgc gcttccgtga actgactgtg ctacgcgttg
    33361 agcgcatcag cgccggtttt cagcgcattg tccttggcgg cgaggcgttg gacggtttta
    33421 catcgcgcgg ctttgacgat cacagcaaac tcttctttcc tcaacctgac gctcactttg
    33481 tgccgccaac ggtaacggaa gagagcatcg tctggccgga aggaccacgc ccaccgtcgc
    33541 gtgactatac gccactgtat gacgaactac gccatgaact ggcgatcgat ttctttattc
    33601 acgacggtgg ggtcgccagc ggctgggcga taaacttacg gtggcaggtc cgcgcggttc
    33661 gctggtggtg ccggaagatt acgcgtatca gctgtatgtc tgcgatgaat ccggaatgcc
    33721 cgcactgcgc cgccgcctgg aaacgttgag caaacttgcc gttaaaccgc aagttagtgc
    33781 gctggttagc gtgcgggata acgcctgtca ggattatctc gcgcaccttg atggttttaa
    33841 tatcgaatgg ctggcacatg atgagcaggc ggtagatgcg cgtctggcgc agatgcaaat
    33901 ccctgccgat gattacttca tctggataac cggcgaaggt aaagtcgtta agaatttaag
    33961 ccgccgcttt gaagcggaac agtatgaccc acagcgggta cgtgcagcgg cttactggca
    34021 cgcaaagt
//
LOCUS       DAJTUI010000036        33649 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTUI010000036 DAJTUI010000000
VERSION     DAJTUI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.036"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..856
                     /gene="corA"
                     /locus_tag="OO464_001875"
     CDS             <1..856
                     /gene="corA"
                     /locus_tag="OO464_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR6227040.1"
                     /translation="EPDDDERLRVQSELGQSLATRPELEDIEASARFFEDDDGLHIHS
                     FFFFEDAEDHAGNSTVAFTIRDGRLFTLRERELPAFRLYRMRARSQSMVDGNAYELLL
                     DLFETKIEQLADEIENIYSDLEQLSRVIMEGHQGDEYDEALSTLAELEDIGWKVRLCL
                     MDTQRALNFLVRKARLPGGQLEQAREILRDIESLLPHNESLFQKVNFLMQAAMGFINI
                     EQNRIIKIFSVVSVVFLPPTLVASSYGMNFEFMPELKWSFGYPGAIIFMILAGLAPYL
                     YFKRKNWL"
     gene            complement(897..1277)
                     /locus_tag="OO464_001876"
     CDS             complement(897..1277)
                     /locus_tag="OO464_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR6227041.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(1291..1707)
                     /locus_tag="OO464_001877"
     CDS             complement(1291..1707)
                     /locus_tag="OO464_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227042.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(1766..2656)
                     /gene="rarD"
                     /locus_tag="OO464_001878"
     CDS             complement(1766..2656)
                     /gene="rarD"
                     /locus_tag="OO464_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6227043.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(2708..3175)
                     /locus_tag="OO464_001879"
     CDS             complement(2708..3175)
                     /locus_tag="OO464_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6227044.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3340..4209
                     /gene="pldA"
                     /locus_tag="OO464_001880"
     CDS             3340..4209
                     /gene="pldA"
                     /locus_tag="OO464_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6227045.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            4342..6171
                     /gene="recQ"
                     /locus_tag="OO464_001881"
     CDS             4342..6171
                     /gene="recQ"
                     /locus_tag="OO464_001881"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6227046.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            6235..6855
                     /gene="rhtC"
                     /locus_tag="OO464_001882"
     CDS             6235..6855
                     /gene="rhtC"
                     /locus_tag="OO464_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR6227047.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6917..7537)
                     /gene="rhtB"
                     /locus_tag="OO464_001883"
     CDS             complement(6917..7537)
                     /gene="rhtB"
                     /locus_tag="OO464_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6227048.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7648..8670
                     /gene="pldB"
                     /locus_tag="OO464_001884"
     CDS             7648..8670
                     /gene="pldB"
                     /locus_tag="OO464_001884"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6227049.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8678..9478
                     /gene="yigL"
                     /locus_tag="OO464_001885"
     CDS             8678..9478
                     /gene="yigL"
                     /locus_tag="OO464_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6227050.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9554..10453
                     /gene="bioP"
                     /locus_tag="OO464_001886"
     CDS             9554..10453
                     /gene="bioP"
                     /locus_tag="OO464_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR6227051.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(10341..11294)
                     /gene="metR"
                     /locus_tag="OO464_001887"
     CDS             complement(10341..11294)
                     /gene="metR"
                     /locus_tag="OO464_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6227052.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11530..13791
                     /gene="metE"
                     /locus_tag="OO464_001888"
     CDS             11530..13791
                     /gene="metE"
                     /locus_tag="OO464_001888"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6227053.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(13830..14645)
                     /locus_tag="OO464_001889"
     CDS             complement(13830..14645)
                     /locus_tag="OO464_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6227054.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            14907..15668
                     /gene="udp"
                     /locus_tag="OO464_001890"
     CDS             14907..15668
                     /gene="udp"
                     /locus_tag="OO464_001890"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6227055.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            15809..17236
                     /gene="rmuC"
                     /locus_tag="OO464_001891"
     CDS             15809..17236
                     /gene="rmuC"
                     /locus_tag="OO464_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6227056.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            17331..18086
                     /gene="ubiE"
                     /locus_tag="OO464_001892"
     CDS             17331..18086
                     /gene="ubiE"
                     /locus_tag="OO464_001892"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6227057.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18100..18705
                     /gene="ubiJ"
                     /locus_tag="OO464_001893"
     CDS             18100..18705
                     /gene="ubiJ"
                     /locus_tag="OO464_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6227058.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            18702..20342
                     /gene="ubiB"
                     /locus_tag="OO464_001894"
     CDS             18702..20342
                     /gene="ubiB"
                     /locus_tag="OO464_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6227059.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            20421..20690
                     /gene="tatA"
                     /locus_tag="OO464_001895"
     CDS             20421..20690
                     /gene="tatA"
                     /locus_tag="OO464_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6227060.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            20694..21209
                     /gene="tatB"
                     /locus_tag="OO464_001896"
     CDS             20694..21209
                     /gene="tatB"
                     /locus_tag="OO464_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6227061.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            21212..21988
                     /gene="tatC"
                     /locus_tag="OO464_001897"
     CDS             21212..21988
                     /gene="tatC"
                     /locus_tag="OO464_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6227062.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22030..22812
                     /gene="tatD"
                     /locus_tag="OO464_001898"
     CDS             22030..22812
                     /gene="tatD"
                     /locus_tag="OO464_001898"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6227063.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(22809..23297)
                     /gene="rfaH"
                     /locus_tag="OO464_001899"
     CDS             complement(22809..23297)
                     /gene="rfaH"
                     /locus_tag="OO464_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6227064.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            23464..24957
                     /gene="ubiD"
                     /locus_tag="OO464_001900"
     CDS             23464..24957
                     /gene="ubiD"
                     /locus_tag="OO464_001900"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6227065.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25003..25704
                     /gene="fre"
                     /locus_tag="OO464_001901"
     CDS             25003..25704
                     /gene="fre"
                     /locus_tag="OO464_001901"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6227066.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(25896..27059)
                     /gene="fadA"
                     /locus_tag="OO464_001902"
     CDS             complement(25896..27059)
                     /gene="fadA"
                     /locus_tag="OO464_001902"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6227067.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27069..29258)
                     /gene="fadB"
                     /locus_tag="OO464_001903"
     CDS             complement(27069..29258)
                     /gene="fadB"
                     /locus_tag="OO464_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6227068.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            29448..30779
                     /gene="pepQ"
                     /locus_tag="OO464_001904"
     CDS             29448..30779
                     /gene="pepQ"
                     /locus_tag="OO464_001904"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6227069.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            30779..31393
                     /locus_tag="OO464_001905"
     CDS             30779..31393
                     /locus_tag="OO464_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6227070.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            31432..32883
                     /gene="trkH"
                     /locus_tag="OO464_001906"
     CDS             31432..32883
                     /gene="trkH"
                     /locus_tag="OO464_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6227071.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            32895..33440
                     /gene="hemG"
                     /locus_tag="OO464_001907"
     CDS             32895..33440
                     /gene="hemG"
                     /locus_tag="OO464_001907"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6227072.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7920 a         8499 c         9114 g         8116 t
ORIGIN      
        1 cgaaccggac gacgacgagc gactgcgcgt acaatctgaa cttggccaga gcctggcaac
       61 ccgcccggaa ctggaagaca tcgaagcatc ggcacgtttc tttgaagacg acgacggcct
      121 gcatattcac tccttcttct tctttgaaga tgcggaagat cacgccggta actccactgt
      181 ggcatttacc atccgtgatg gtcgtctgtt tactctgcgt gagcgtgaac tgcccgcttt
      241 tcgtctgtat cgtatgcgtg cccgtagcca gtcgatggta gacggtaacg cctacgagtt
      301 gctgctggat ctgttcgaaa ccaaaatcga acagttggca gatgaaattg aaaatatcta
      361 tagcgacctg gagcagttga gccgggtgat tatggaaggg catcagggcg atgagtacga
      421 cgaggcgctc tccacgctgg cggaactgga agatatcggc tggaaagttc gcctgtgtct
      481 gatggatacc cagcgcgctc tcaacttcct ggtgcgtaaa gcgcgtttac cgggtgggca
      541 actggagcag gcgcgtgaaa tcctgcgcga tatcgaatcc ctgctgccgc ataacgaatc
      601 cctgttccag aaggtgaact tcctgatgca ggcggcaatg ggttttatca acatcgagca
      661 gaaccgcatc atcaaaattt tctcggtggt atccgtcgtg ttcctgccgc caacgctggt
      721 tgcttccagc tacgggatga actttgagtt tatgccagaa ctgaagtgga gtttcggcta
      781 ccctggcgcg attatcttta tgatcctcgc gggcctggca ccgtatctgt actttaagcg
      841 gaagaactgg ttgtaaaaag aaggcggtgg cttagcctgg ctaagccacc tgttattcaa
      901 aggctccagg tatttaaccc ttttacctct ttctcataaa accatttgtt cgtgttaaca
      961 gcaacatagg ctgctacggc aattcccaga atgttaacgc caattaatgc accaacaaat
     1021 agagatagaa tgccaagtaa tagaactata attgcttttt tccataagcc cagaacaaat
     1081 aaatagatcc aggaacagaa gaaagcaatg aaattcattt gaatagttaa tcgttgtctt
     1141 acttttaatg ctttaaatgc tgctttatat tctggtgttg ctccccagaa tccaggaaag
     1201 ccatgttgat cataaaaatt aaatcggtat ttccattttt cacttaatga atcatcgttc
     1261 atatattcct tactcataaa tactccataa ctattgtttt gatgaatcag taggtgcaag
     1321 cattagcata ctgaaagtgg aaaaataaca aatcaaaaaa atcatcgaac cattgcctga
     1381 acaggcaaaa tcttcggcta tcattgtgat gatagagatg atatatactg ctaatgtacc
     1441 aaaaacataa atttttatat atatgaaacc actatcacgg agtcgttggc aattcatgtt
     1501 gatgacgaga taatggagtg cgatggtaga gactataaca agaaaacctg cttccccatc
     1561 gttaaaaaag ataataagaa aggcaaaaat gaaatttatt aaaataaata aaaatatata
     1621 tcggtgtctg gaaatcttac cgttagatgt tgggataaac atacgtaaca taaattttac
     1681 atccttgtgt gagtctcctg tcagcatggc aatatgccca ctctcataca agagtcggca
     1741 tattttttca gaatatattt attttttatt tggacgttct acgctgcgta taaatcgcgt
     1801 ccatcacaaa aattgccagc gccacccaaa taaaggtgaa agtcaccatc ttatcggcac
     1861 ccggtttttc accataaaac gtcacagcca gcaggaacat cagcgtcggg ccaatgtact
     1921 gaaaaaagcc taacgttgag agacgcaggc gcgtggcagc ggcagtaaaa cacaacagcg
     1981 gtacggtagt gacaataccg gcggcgatca gcagtaaatt cagcgacatc gggttttgcc
     2041 ccatatggct ggttgaactg tcggcaatag caaacaggta aatcgccgcc acgggcagca
     2101 gccacatggt ttcaattaac atgccggttt gtgcttctac cgcaatcttc ttgcgcacca
     2161 ggccgtagaa ggcaaaacta aatgccagcc ccagcgcgat aataggtagc gaaccaaaag
     2221 tccacagctg gaccaacacg ccacatatcg ccagaatcac cgccagccat tgcatccggc
     2281 ggaatcgctc gccgaggaaa atcatcccca gcacaatgtt caccagcggg ttaataaagt
     2341 aaccaaggct cgcttccagc atatggtgat tgttcaccgc ccagataaac agtagccagt
     2401 tgccaccaat cagtacggca gagactgcca gcataaaaat tttctgtggc gtctgaatca
     2461 gcgtttttaa ataggaccac tggcggcaaa tgctcatcag caccaccata aagaaaaacg
     2521 accagatcac gcgatgcgtc aggatctcat cggcgggcac gtagtaaatc agcttgaagt
     2581 atgctggcgc aataccccaa ataaaataag cggcaagagc gagtaatacg ccctgccgcg
     2641 tttgttttgc atccatcggg aatactcatt tttaattggt aacagcagtt tacctgcttt
     2701 tatgtcttca acctaccata taggtggcgg tggcactggc aatataaagc tgttcttcat
     2761 tgtgtaattc aacgcgggcg acggcgactt tattgcctgc acgcaacagg ctactggtag
     2821 cagtaaaacg ctcgcccctg cctgggcgca gataatcaac gcgaagatca atggtcccca
     2881 tccgcgatag ccgctggcgt agttcatctt cactgatggt ttcgtggcgg gttaaggtac
     2941 ttcccacgca caccagaccg gcggcgacat ccagcgccga cgcaatgact ccgccgtgca
     3001 aaatgctttg cgcccagtta cccaccatca tcggctgatt tttaaaggcc agctgtgcga
     3061 actctttttc gtaacgctcc agttccatcc ccaatgcgcg gttaaatggc atgtgataaa
     3121 caaacatctc acccactaat ttcagggctt gttcagcggt cagtacggca gacatatcat
     3181 ccttacactt cattggttaa tgaaatgttg attttatgct tctttgttgt tggtttctac
     3241 tttaggaagg gataactaac ggcaatggag ttaagtatgt aaaatagccc gcagaaaaac
     3301 attcacctta tcaataattc gttacggaga acacgaccga tgcggactct gcagggctgg
     3361 ttgttgccgg tgtttatgtt gcctatggca gtatatgcac aagaggcaac ggtgaaagag
     3421 gtgcatgacg cgccagcggt gcgtggcagt attatcgcca atatgctgca ggagcatgac
     3481 aatccgttca cgctctatcc ttatgacacc aactacctca tctacaccca aaccagcgat
     3541 ctgaataaag aagcgattgc cagttacgac tgggcggaaa atgcgcgtaa ggatgaagta
     3601 aagtttcagt tgagcctggc atttccgctg tggcgtggga ttttaggccc aaactcggtg
     3661 ttgggtgcgt cttatacgca aaaatcctgg tggcaactgt ccaatagcga agagtcttca
     3721 ccgtttcgtg aaaccaacta cgaaccgcaa ttgttcctcg gttttgccac cgattaccgt
     3781 tttgcaggtt ggacgctgcg cgatgtggag atggggtata accacgactc taacgggcgt
     3841 tcagacccga cctcccgcag ctggaaccgc ctttatactc gcctgatggc agaaaacggt
     3901 aactggctgg tagaagtgaa gccgtggtat gtggtgggta atactgacga taacccggat
     3961 atcaccaaat atatgggtta ctaccagctt aaaatcggct atcacctcgg tgatgcggtg
     4021 ctcagtgcga aaggacagta caactggaac accggctacg gcggcgcgga gttaggctta
     4081 agttacccga tcaccaaaca tgtgcgcctt tatactcagg tttacagcgg ctatggcgaa
     4141 tcgctcatcg actataactt caaccagacc cgtgtcggtg taggggttat gctaaacgac
     4201 ttgttttgat gaacggttga gtggttggca aatctggaat ccagcatcca ggattaccct
     4261 ctcagagact aaaagcattg cagtttctcg cgcaggcgct gaaaatagcg cctgttttta
     4321 tttcaggcaa tcggggtgaa tgtggcgcag gcggaagtgt tgaatctgga gtccggagct
     4381 aaacaggttt tacaagaaac ctttggctac caacagtttc gccccggcca ggaagaaatt
     4441 atcgacactg tgctttccgg tcgcgattgc ctggttgtta tgcccactgg tggcggaaaa
     4501 tccctttgct atcaaattcc tgccttattg ctaaacggcc ttaccgtggt tgtttcaccg
     4561 ctgatttcgt tgatgaaaga tcaggtggat caactacagg ccaacggcgt ggcggcggcg
     4621 tgccttaact cgacgcaaac ccgcgaacag caacttgaag tgatgacagg ctgccgcacc
     4681 gggcaaattc gcttactgta tatcgccccg gaacgcctga tgctggataa ctttcttgag
     4741 catctggcgc actggaatcc ggtgttatta gccgtcgatg aagcgcactg tatctcccaa
     4801 tggggtcacg atttccgccc ggaatatgcc gcgctcggtc agttgcgcca gcggttcccg
     4861 acgctgccgt ttatggcgct gaccgccaca gccgacgaca ccacgcgcca ggatatcgtg
     4921 cgcctgctgg ggctgaacga tccgctgatt caaatcagca gttttgaccg tccgaatatt
     4981 cgctacatgc tgatggagaa attcaaaccg ctcgatcagt tgatgcgcta cgtgcaggaa
     5041 cagcgcggta agtctggcat tatctactgc aacagccggg cgaaagtaga agacaccgct
     5101 gcgcgcctgc aaagcagggg tattagcgcg gcggcctatc atgccgggct ggaaaataat
     5161 gtccgcgccg acgtgcagga gaaattccag cgcgatgatc tgcaaattgt ggtggcgacg
     5221 gtggcgttcg gcatgggcat caataaacca aacgttcgct tcgtggtcca ctttgatatt
     5281 ccgcgcaata tcgaatccta ttatcaggaa accggtcgcg ccgggcgtga tggcctgcct
     5341 gcggaagcga tgctgtttta cgatccggct gatatggcgt ggctgcgccg ttgtctggaa
     5401 gagaagccgc aggggcagtt gcaggatatc gagcgccaca aactcaatgc gatgggcgcg
     5461 tttgccgaag cgcaaacttg ccgtcgtctg gtattgctga actattttgg cgaagggcgt
     5521 caggagccgt gcgggaactg cgatatctgc ctcgatccgc cgaaacagta cgacggttca
     5581 accgatgctc agattgccct ttccaccatt ggtcgtgtga atcagcggtt tgggatgggt
     5641 tatgtggtgg aagtgattcg tggtgctaat aaccagcgta tccgcgacta tggtcatgac
     5701 aaactgaaag tctatggtat gggccgtgat aaaagccatg aacattgggt gagcgtgatc
     5761 cgccagctga ttcatctcgg cctggtgacg caaaatattg cccagcattc tgccctacaa
     5821 ctgacagagg ccgcgcgccc ggtgctgcgc ggcgaatcct ctttgcaact tgccgtgccg
     5881 cgtatcgtgg cgctcaaacc gaaagcgatg cagaaatcgt tcggcggcaa ctatgatcgc
     5941 aaactgttcg ccaaattacg caaactgcgt aaatcgattg ccgatgaaag taatgtcccg
     6001 ccgtacgtgg tgtttaacga cgcaaccttg attgagatgg ctgaacagat gccgatcacc
     6061 gccagcgaaa tgctcagcgt taacggcgtt gggatgcgca agctggaacg ctttggtaaa
     6121 ccgtttatgg cgctgattcg tgcgcatgtc gatggcgacg acgaagagta gtcagcagca
     6181 taaaaaagtg ccagtatgaa gactccgtaa acgtttcccc cgcgagtcaa atgtatgttg
     6241 atgttatttc tcaccgtcgc catggtgcac attgtggcgc ttatgagccc cggtcccgat
     6301 ttcttttttg tctctcagac cgctgtcagt cgttcccgta aagaagcgat gatgggcgtg
     6361 ctgggcatta cctgcggcgt aatggtttgg gctgggattg cgttgcttgg cctgcatttg
     6421 attatcgaaa aaatggcctg gctgcatacg ctgattatgg taggcggtgg cctgtatctc
     6481 tgctggatgg gttaccagat gctacgcggt gcactgaaaa aagaggctgt atctgcgcct
     6541 gcaccacagg ttgagctggc gaaaagcggg cgcagtttcc tgaaaggctt actgaccaat
     6601 ctcgccaatc cgaaagcgat tatctatttt ggttcggtat tctcattgtt tgtcggcgat
     6661 aacgttggca ctaccgagcg ctggggcatt ttcgcgttga ttattgtcga aacactggcg
     6721 tggtttaccg tcgttgccag tctgtttgcc ctgccgcaaa tgcgccgtgg ttatcaacgt
     6781 ctggcgaagt ggattgatgg ttttgccggg gcgttatttg ccggatttgg cattcatttg
     6841 attatttcgc ggtgatgcca gacgcgtctt cagagtaagt cggataaggc gtttacgccg
     6901 catccgacat tatttttcac gcatgcctcg ccgatgctaa cagcgctccc accagcataa
     6961 acaacgagcc gaaaatctta ttcagcgcct tcatctgctt tggtccttta atccatagag
     7021 caatcctttg agcaagggtg gcgtaaccga tcatcacaat aatatcgacc acaatagtgg
     7081 tgacgccgag cacgatatac tgcatcagtt gcggctgttg cggcatgatg aattgcggaa
     7141 atagcgccgc cagaaacaca atacttttgg gattggtgag attcacaaaa actgcgcgct
     7201 ggaacaaatg tcgacgcgat tgagtagagg ccagcgattt aagatcaatt gcgccagcgg
     7261 cgcgccactg ctggattccc agccaaatca agtaagccgc gcctgcccac tttaacactt
     7321 caaacgcaat cactgagcgg gaaaatagcg tccccaaccc cacgccaacc agcacaatat
     7381 gaatcgccag tccggtctga agcccagcaa tagacgccac cgcgccgcga taaccgtggt
     7441 tgagcgaggt ggtcatagtg ttgattgcac cagagcctgg cgacaggctt aaaatgatcg
     7501 atgtcagcag gtaggcaaac caccattcta aggtcatgat gaactcccgg tgtgtctatt
     7561 tttgtgccac aatacgctac tgtcgcagcg ttgtgtcagg cacgcttaaa aaaacgattt
     7621 tacgtggttt aagaggcaga ttacccgatg tttcagcagc aaaaagactg ggaaacaaga
     7681 gaaaacgcgt ttgctgcttt taccatggga ccgctgactg atttctggcg tcagcgtgat
     7741 gaagcagagt ttactggtgt ggatgacatt ccggtgcgct ttgtccgttt tcgcgcacag
     7801 caccatgacc gggtggtagt catctgcccg gggcgtattg agagctacgt aaaatatgcg
     7861 gaactggcct acgacctgtt ccatttgggg tttgatgtct taatcatcga ccatcgcggg
     7921 cagggacgtt ccggtcgcct gttagccgat ccgcatctcg ggcatgttaa tcgctttaat
     7981 gattatgttg atgatctggc ggcattctgg cagcaggagg ttcagcccgg tccgtggcgt
     8041 aaacgctata tactggcaca ttcgatgggc ggtgcgatct ccacattatt tctgcaacgc
     8101 catccaggtg tatgtgacgc cattgcgcta actgcgccaa tgtttgggat cgtgattcgt
     8161 atgccgtcat ttatggcacg gcagatcctc aactgggccg aagcgcatcc acgtttccgt
     8221 gatggctatg caataggcac cgggcgctgg cgcgcgttgc cgtttgctat caacgtactg
     8281 acccacagca gacagcgata tcgacgtaac ttacgcttct atgctgatga cccaacgatt
     8341 cgcgtcggtg ggccgaccta ccattgggta cgcgaaagta ttctggctgg cgaacaggtg
     8401 ttagccggtg cgggtgatga cgccacgcaa acgcttctct tacaagctga agaggaacgc
     8461 gtggtggata accgcatgca cgaccgtttt tgtgaactcc gcaccgccgc gggccatcct
     8521 gtcgaaggag gacggccgtt ggtaataaaa ggtgcttacc atgagatcct ttttgaaaag
     8581 gacgcaatgc gctcagtcgc gctccacgcc atcgttgatt ttttcaacag gcataactca
     8641 cccagcggaa accgctctac agaggtttaa atttcttatg taccaggttg ttgcgtctga
     8701 tttagatggc acgttacttt ctcccgacca tacgttatcc ccttacgcca aagaaactct
     8761 gaagctgctc accgcgcgcg gcatcaactt tgtgtttgcg accggtcgtc accacgttga
     8821 tgtggggcaa attcgcgata atctggagat taagtcttac atgattacct ccaatggtgc
     8881 gcgcgttcac gatctggatg gcaatctgat ttttgctcat aacctggatc gcgacattgc
     8941 cagcgatctg tttggcgtag tcaacgacaa tccggacatc attactaacg tttatcgcga
     9001 cgacgaatgg tttatgaatc gccatcgccc ggaagagatg cgctttttta aagaagcggt
     9061 gttcaaatat gcgctgtatg agccgggatt actggagccg gaaggcatca gcaaagtgtt
     9121 tttcacctgc gattcccatg aacaactgct gccgctggag caggcgatta acgctcgctg
     9181 gggcgatcgc gtcaacgtca gtttctctac cttaacctgt ctggaagtga tggcgggcgg
     9241 cgtgtcgaaa ggccatgcgc tggaagcagt ggcgaagaaa ctgggctaca gcctgaagga
     9301 ttgtattgcg tttggtgacg ggatgaacga cgccgaaatg ctgtcgatgg cggggaaagg
     9361 ctgcattatg ggcagtgcgc accagcgtct gaaagatctg catccggagc tggaagttat
     9421 tggtactaat gccgacgacg cggtgccgca ttatctgcgt aaactctatt tatcgtaatc
     9481 gttctttatt tggtcagttg tcaacctgat acttcgctac aatggatact cgttaatcaa
     9541 agagttttcc attgtggcgt tacttatcat caccacgatt ctgtgggcct tctcctttag
     9601 cttttatggc gagtaccttg cggggcacgt cgatagctat tttgcggtgc tggtgcgcgt
     9661 tggcttggcg gcactcgttt ttctgccgtt tctgcgtacc cgtagcaata gcctgaaaac
     9721 ggtcggcctg tatatgctgg tgggcgcgat gcagctcggc gtgatgtata tgctgagttt
     9781 ccgcgcttat ctctacctga cggtctccga attgttgctg ttcaccgtac tgacgccgct
     9841 ctacatcacg ctgatttatg acatcatgag taagcgccgt ctgcgctggg gctatgcttt
     9901 tagcgccttg ctggcggtga ttggtgccgg gattattcgc tatgatcagg tcaccgacca
     9961 tttctggact ggcttgttgc tggtgcaact ctccaatatc acttttgcca tcggcatggt
    10021 gggttacaaa cgcctgatgg aaactcgccc gatgccgcag tataacgcct ttgcgtggtt
    10081 ctatcttggc gcgttcctgg tggcggtaat tgcatggttc ttgctgggaa atgcgcagaa
    10141 aatgccgcaa accacgttgc aatggggcat tctggtgttt cttggcgtgg tggcttccgg
    10201 gattggctac tttatgtgga actacggcgc gacgcaggtg gacgccggaa cgctgggcat
    10261 tatgaataat atgcacgttc cggcagggct gctggtaaac ctggctatct ggcaccaaca
    10321 gccgcactgg ccaacgttta ttacaggcgc gctggtgatc ctggcctcac tgtgggtgca
    10381 tcgtaagtgg gtcgctccgc gctcttcaca aacggcagat gatcgcaggc gtgattgcgc
    10441 gctgagcgaa taaacgcttc cgtaactggc tgacgctgct cgccatcgcg cacggcggcg
    10501 tacagtcggc tccacaagcc ttcgcccagg gttttggtca ccaccagacc ctggcgctca
    10561 aaactctcta ctacccaatg cggtagcgcg gcaataccca tccgcgcggc aaccatttga
    10621 atcaacaata aggtgttatc gacgcttttc agtgacgggc tgacgcctgc cggctgaaga
    10681 aaatgccgcc agacatccag tcgactacgc tgcaccggat aaattaatag cgtctcgctg
    10741 gcgagatctt ccggtgtaat acgcgttttt gccgccagtg gatggtcagg tgctaacacc
    10801 aggcgtactt cataatcgaa catcggcgaa taatgcaggc cactgcgcgg caggatatcg
    10861 gaggtcatca ccagatccag ctcgccctgt tgcaacgcgg gctgcgggtc aaacgtcacg
    10921 cccgatttaa aatccatctc tacctgcggc cagttcttat ggaaatttcc taaagcgggt
    10981 gtcagccact gaatacagct atggcactca atggcaatgc gcagacgcgt ctgctgcggt
    11041 tcattgcagg cttgcagtgc ctggctgatt tgcggcagca cctggtttgc cagttgcaac
    11101 aggatttctc cctgcggtgt aaagcgtagc ggctggctct tacgcacaaa tagccggaag
    11161 ccaaggcgtt gttccagatc gctaaactgg tgagacaggg cggattgcgt ctgatgcaac
    11221 gttgccgcag cggctgcgag cgagccgcag ttccgcaacg cttgtagcgt tttcaggtgt
    11281 tttacttcga tcatgaaagt ccttcacttc ggcatgaata atttgcgctt gaggaatata
    11341 cagtaaccgc caattatgga tgtgtaaaca tctggacggc taaaatcatt cgtcttttaa
    11401 atttatggtg cgttggctgc gtttctccac cccggtcact tacttcagta agctcccggc
    11461 gatgaataaa cttgccgcct tccctaaatt caaaatccat aggatttaca tataattaga
    11521 ggaagaaaaa tgacaatatt gaatcacacc ctcggtttcc ctcgcgttgg cctgcgtcgc
    11581 gagctgaaaa aagcgcaaga aagttattgg gcggggaact ccacgcgtga agaactgctg
    11641 acagtagggc gtgaactgcg tgctcgtcac tgggatcaac aaaagcaagc gggtatcgac
    11701 ctgctgccgg tgggcgattt tgcctggtac gatcatgtac tgaccaccag tttgctgctg
    11761 ggtaatgttc cgccacgtca tcagaacaaa gacggttcgg tagatatcga caccctgttc
    11821 cgtattggtc gtggacgtgc gccgactggc gaacctgcgg cggcagcgga aatgaccaaa
    11881 tggtttaaca ccaactatca ctacatggtg ccggagttcg ttaaaggcca acagttcaaa
    11941 ctgacttgga cgcagctgct ggaggaagtg gacgaggcgc tggcgctgag ccacaacgtt
    12001 aagcctgtgc tgctggggcc ggttacttac ctgtggctgg ggaaagtgaa aggtgaacag
    12061 tttgaccgcc ttagcctgct gaacgacatt ctgccggttt atcagcaagt gctggcagaa
    12121 ctggcgaaac gcggcatcga gtgggtacag attgatgaac ccgcgctggt actggaacta
    12181 ccacaggcgt ggctggacgc atacaaaccc gcttacgacg cgctccaggg acaggtgaaa
    12241 ctgctgctga ccacctattt tgaaggcgta acgccaaatc tcgacacgat tactgcgctg
    12301 cctgttcagg ggctgcatgt ggaccttgta catggtaaag ataacgttgt tgaactgcac
    12361 aagcgcctgc cttctgactg gctgctgtct gcgggtctga tcaatggtcg taacgtctgg
    12421 cgcgccgatc ttactgagaa atatgcgcaa attaaggaca ttgtcggcaa acgtgatttg
    12481 tgggtggcat cttcctgctc gttgctgcac agccccatcg acctgagcgt ggaaacgcgt
    12541 cttgatgcag aagtgaaaag ctggtttgcc ttcgccctac aaaaatgcca tgaactggca
    12601 ctgctgcgcg atgcgctgaa cagtggtgac acggcagctc tggcagagtg gagcgcccca
    12661 attcaggcgc gtcgtcactc tactcgtgta cataatccgg cggtagaaaa gcgtctggcg
    12721 gcgatcaccg cccaggacag ccagcgtgcg aatgtctatg aagtgcgtgc tgaagcccag
    12781 cgtgcgcgtt ttaaactgcc agcgtggccg accaccacga ttggttcctt cccgcaaacc
    12841 acggaaattc gtaccctgcg tctggatttc aaaaagggca atctcgacgc caacaactac
    12901 cgcacgggca ttgcggaaca tatcaagcag gccattgttg agcaggaacg tttgggactg
    12961 gatgtgctgg tacatggcga ggccgagcgt aatgacatgg tggaatactt tggcgagcac
    13021 ctcgacggat ttgtctttac gcaaaacggt tgggtacaga gctacggttc ccgctgcgtg
    13081 aagccaccga ttgtcattgg tgacattagc cgcccggcac cgattaccgt ggagtgggcg
    13141 aagtatgcgc aatcgctgac cgacaaaccg gtgaaaggga tgctgacggg gccggtgacc
    13201 atactctgct ggtcgttccc gcgtgaagat gtcagccgtg aaaccatcgc caaacagatt
    13261 gcgctggcgc tgcgtgatga agtggccgat ctggaagccg ctggaattgg catcatccag
    13321 attgacgaac cggcgctgcg cgaaggttta ccgctgcgtc gtagcgactg ggatgcgtat
    13381 ctccagtggg gcgtagaggc cttccgtatc aacgccgccg tggcgaaaga tgacacacaa
    13441 atccacactc acatgtgtta ttgcgagttc aacgacatca tggattcgat tgcggcgctg
    13501 gacgcagacg tcatcaccat cgaaacctcg cgttccgaca tggagttgct ggagtcgttt
    13561 gaagagtttg attatccaaa tgaaatcggt cctggcgtct atgacattca ctcgccaaac
    13621 gtaccgagcg tggaatggat tgaagccttg ctgaagaaag cggcaaaacg cattccggca
    13681 gagcgcctgt gggtcaaccc ggactgtggc ctgaaaacgc gcggctggcc agaaacccgc
    13741 gcggcactgg cgaacatggt gcaggcggcg cagaatttgc gtcgggggta aatccaaacc
    13801 gggtggtaat accacccggt cttttctcat tacagcgact tcttcccacc atactgctta
    13861 aaccattcca gcatacgctg ccagccatct ttcgcagatt cggcatgata gctcgggcga
    13921 taatcagcgt tgaatgcatg cccggcatcc gggtacacga taatctctgc tttcgcatta
    13981 gcagcccgca gcgcctggcg catcgtttca acgctctcct gcggaatgct gttatcctga
    14041 ccaccatata agccgagaac cggcgcgtta agatcggttg cgatatcaac aggttgtttc
    14101 ggtgaattca gcgacttgtc gcccgtcagt ttgccgtacc acgccactgc ggcttttagc
    14161 tgtggattat gcgcggcata cagccaggtg atacgtccac cccagcagaa tccggtgatc
    14221 attaaacgat gaacatcgcc gccgttgcgg gaagcccaac tggcgacatg atcgagatcg
    14281 gccagcacct gcgagtcagg cacttttgct accagaccgc taagcaacgt ggggatatcg
    14341 gcaaaatcat tcggatcgcc ttcgcggaag taaagttcag gtgcgatagc cagatacccc
    14401 tccagcgcca gacggcgaca aatgtcgcgg atatgttcat gcacgccaaa aatttcctgc
    14461 actacaatga ccactggcag tgggccatcg ctttgctttg gtctggcatg gtaagcaggc
    14521 atgttatccc cttgtgaagg gatagaggtg aagcccgcca caatcgcgtc gtccggggtc
    14581 tgaacgatgg tcgaagcgag aggcgatgca gcaggtgcaa atccagattg ttgtgttgtt
    14641 gccatggtat tctccgtacc cttataaaaa tgttgcgcaa tgttaactat agtcagcatg
    14701 caacaaatca cattgcctga atcggctcat cttttatgca gtcctgcaga atgaagagtg
    14761 atttatgtga tatgaatcac ttttggtggg taaatttatg caacgcattt gcgtcatggt
    14821 gatgagtatc acgaaaaaat gttaaaccct tcggtaaagt gtctttttgc ttcttctgac
    14881 taaaccgatt cacagaggag ttgtatatgt ccaagtctga tgtttttcat ctcggcctca
    14941 ctaaaaacga tttacaaggg gctacgcttg ccatcgtccc tggcgacccg gatcgtgtgg
    15001 aaaagatcgc cgcgctgatg gataagccgg ttaagctggc atctcaccgc gaattcacta
    15061 cctggcgtgc agagctggat ggtaaacctg ttatcgtctg ctctaccggt atcggcggcc
    15121 cgtctacctc tattgctgtt gaagagctgg cacagctggg cattcgcacc ttcctgcgta
    15181 tcggtacaac gggcgctatt cagccgcata ttaatgtggg tgatgtcctg gttaccacgg
    15241 cgtctgtccg tctggatggc gcgagcctgc acttcgcacc gctggaattc ccggctgtcg
    15301 ctgatttcga atgtacgact gcgctggttg aagctgcgaa atctattggc gcgacaactc
    15361 acgttggcgt gacagtttct tctgatacct tctacccagg tcaggaacgt tacgatactt
    15421 actctggtcg cgtagttcgt cactttaaag gttctatgga agagtggcag gcgatgggcg
    15481 taatgaacta tgaaatggaa tctgcaactc tgctgaccat gtgtgcaagt cagggcctgc
    15541 gtgccggtat ggtagcgggt gttatcgtta accgcaccca gcaagagatc ccgaatgctg
    15601 agacgatgaa acaaaccgaa agccatgcgg tgaaaatcgt ggtggaagcg gcgcgtcgtc
    15661 tgctgtaatt ctcttctcct gtctgaaggc cgacgcgttc ggccttttgt atttttgcgt
    15721 agcgcctcgc aggaaatgcc tttccaactg gacgtttgta cagcacaatt ctattttgtg
    15781 cgggtaagtt gttgcgtcag gaggcgttgt ggatttctca atcatggttt acgcagttat
    15841 tgcgttggtg ggtgtggcaa ttggctggct gtttgccagt tatcaacatg cgcagcaaaa
    15901 agccgagcaa ttagctgaac gtgaagagat ggtcgcggag ttaagcgcgg caaaacaaca
    15961 aattacccaa agcgagcact ggcgtgcaga gtgtgaattg ctcaataacg aagtgcgcag
    16021 cctgcaaagt attaacacct ctctggaggc cgatctgcgt gaagtaacca cgcggatgga
    16081 agccgcacag caacatgctg acgataaaat tcgccagatg attaacagcg agcagcgcct
    16141 cagtgagcag tttgaaaacc tcgccaaccg tatttttgag cacagcaatc gccgggttga
    16201 tgagcaaaac cgtcagagtc tgaacagcct gttgtcgccg ctacgtgaac aactggacgg
    16261 tttccgccgt caggttcagg acagcttcgg taaagaagca caagaacgcc ataccctgac
    16321 ccacgaaatt cgcaatctcc agcaactcaa cgcgcaaatg gcccaggaag cgatcaacct
    16381 gacgcgcgcg ctgaaaggcg acaataaaac ccagggcaac tggggcgagg tagtattgac
    16441 gcgggtgctg gaggcttccg gtctgcgtga agggtatgaa tatgaaaccc aggtcagcat
    16501 cgaaaatgac gcccgctcgc ggatgcagcc ggatgtcatc gtgcggctgc cgcagggaaa
    16561 agatgtggtg atcgacgcca aaatgacgct ggtcgcctat gaacgctatt ttaacgccga
    16621 agacgactac acccgcgaaa gcgcgctaca ggaacatatc gcgtcggtgc gtaaccatat
    16681 ccgtttgctg ggacgcaaag attatcaaca gctgccgggg ctgcgaactc tggattacgt
    16741 gctgatgttt attcccgttg aacccgcttt tttactggcg cttgaccgcc agccggagct
    16801 gatcaccgaa gcgttgaaaa acaatatcat gctggttagc ccgactacgc tgctggtggc
    16861 gttgcgtact attgccaacc tgtggcgtta tgagcatcaa agccgtaacg cccagcaaat
    16921 cgccgatcgt gccagcaagc tgtacgacaa gatgcgtttg ttcatcgatg acatgtccgc
    16981 gattggtcaa agtctcgaca aagcgcagga taactaccgc caggcaatga aaaaactctc
    17041 ttcaggtcgc ggaaatgtgc tggcgcaggc agaagcgttt cgcggtttag gagtagaaat
    17101 taaacgcgag attaatccgg atttggctga acaggcggtg agccaggatg aagagtatcg
    17161 acttcggtcg gttccggagc agccgaatga tgaagcttat caacgcgatg atgaatataa
    17221 tcagcagtcg cgctagccca ttaggggtag ttaagccggg tagaaatcta gggcatcgac
    17281 gcccaatctg ttacacttct ggaacaattt tttgatgagc aggcattgag atggtggata
    17341 agtcacaaga aacgacgcac tttggttttc agaccgtcgc gaaggaacaa aaagcggata
    17401 tggtcgccca cgttttccat tccgtggcat caaaatacga tgtcatgaat gatttgatgt
    17461 catttggtat tcatcgtttg tggaagcgat tcacgattga ttgcagcggc gtacgccgtg
    17521 ggcagaccgt gctggatctg gctggtggca ccggcgacct gacagcgaaa ttctcccgcc
    17581 tggtcggaga aactggcaaa gtggtccttg ctgatatcaa tgaatccatg ctcaaaatgg
    17641 gacgcgagaa gctgcgtaat atcggtgtga ttggcaacgt tgagtatgtt caggcgaacg
    17701 ctgaggcgct gccgttcccg gataacacct ttgattgcat caccatttcg tttggtctgc
    17761 gtaacgtcac cgacaaagat aaagcactgc gttcaatgta tcgcgtgctg aaacccggcg
    17821 gccgcctgct ggtgcttgag ttctcgaagc caattatcga gccgctgagc aaagcctatg
    17881 atgcatactc cttccatgtg ctgccgcgta ttggctcact ggtcgcgaac gacgccgaca
    17941 gctaccgtta tctggcagaa tccatccgta tgcatcccga tcaggatacc ctgaaagcca
    18001 tgatgcagga tgccggattc gaaagtgtcg actactacaa tctgacggca ggggttgtgg
    18061 cgctgcatcg tggttataag ttctgacagg agaccggaaa tgccttttaa acctttagtg
    18121 acggcaggaa ttgaaagtct gctcaacacc ttcctgtatc gctcacccgc gctgaaaaca
    18181 gcccgctcgc gtctgctggg taaagtattg cgcgtggagg taaaaggctt ttcgacgtca
    18241 ttgattctgg tgttcagcga acgccaggtt gatgtactgg gcgaatgggc aggcgatgct
    18301 gactgcaccg ttatcgccta cgccagtgtg ttgccgaaac ttcgcgatcg ccagcagctt
    18361 accgcactga ttcgcagtgg tgagctggaa gtgcagggcg atattcaggt ggtgcaaaac
    18421 ttcgttgcgc tggcagatct ggcagagttc gaccctgcgg aactgctggc cccttatacc
    18481 ggtgatatcg ccgctgaagg aatcagcaaa gccatgcgcg gaggcgcaaa gttcctgcat
    18541 cacggcatta agcgccagca acgttatgtg gcggaagcca ttactgaaga gtggcgtatg
    18601 gcacccggtc cgcttgaagt ggcctggttt gcggaagaga cggctgccgt cgagcgtgct
    18661 gttgatgccc tgaccaaacg gctggaaaaa ctggaggcta aatgacgcca ggtgaagtac
    18721 ggcgcctata tttcatcatt cgcacttttt taagctacgg acttgatgaa ctgatcccca
    18781 aaatgcgtat caccctgccg ctacggctat ggcgatactc attattctgg atgccaaatc
    18841 ggcataaaga caaactttta ggtgagcgac tacgactggc cctgcaagaa ctggggccgg
    18901 tttggatcaa gttcgggcaa atgttatcaa cccgccgcga tctttttcca ccgcatattg
    18961 ccgatcagct ggcgttattg caggacaaag ttgctccgtt tgatggcaag ctggcgaagc
    19021 agcagattga agctgcaatg ggcggcttgc cggtagaagc gtggtttgac gattttgaaa
    19081 tcaagccgct ggcttctgct tctatcgccc aggttcatac cgcgcgattg aaatcgaatg
    19141 gtaaagaggt ggtgattaaa gtcatccgcc cggatatttt gccggtcatt aaagcggatc
    19201 tgaaacttat ctaccgtctg gctcgctggg tgccgcgttt gctgccggat ggtcgccgtc
    19261 tgcgcccaac cgaagtggtg cgcgagtacg aaaagacctt gattgatgaa ctgaatttgc
    19321 tgcgggaatc tgccaacgcc attcagcttc ggcgcaattt tgaagacagc ccgatgctct
    19381 acatcccgga agtttaccct gactattgta gtgaagggat gatggtgatg gagcgcattt
    19441 acggcattcc ggtgtctgat gttgcggcgc tggagaaaaa cggcactaac atgaaattgc
    19501 tggcggaacg cggcgtgcag gtgttcttca ctcaggtctt tcgcgacagc tttttccatg
    19561 ccgatatgca ccctggcaac atcttcgtaa gctatgaaca cccggaaaac ccgaaatata
    19621 tcggcattga ttgcgggatt gttggctcgc taaacaaaga agataaacgc tatctggcag
    19681 aaaactttat cgccttcttt aatcgcgact atcgcaaagt ggcagagcta cacgtcgatt
    19741 ctggctgggt gccaccagat accaacgttg aagagttcga atttgccatt cgtacggtct
    19801 gtgaacctat ctttgagaaa ccgctggccg aaatttcgtt tggacatgta ctgttaaatc
    19861 tgtttaatac ggcgcgtcgc ttcaatatgg aagtgcagcc gcaactggtg ttactccaga
    19921 aaaccctgct ctatgtcgaa ggggtaggac gccagcttta tccgcagctc gatttatgga
    19981 aaacagcgaa gcctttcctg gagtcgtgga ttaaagatca ggtcggtatt cctgctctgg
    20041 tgagagcatt taaagaaaaa gcgccgttct gggtcgaaaa aatgccagaa ctgcctgaac
    20101 tggtttacga cagtttgcgc cagggcaagt atttacagca cagtgttgat aagattgccc
    20161 gcgagcttca gtcaaatcat gtacgtcagg gacaatcgcg ttattttctc ggaattggcg
    20221 ctacgttagt attaagtggc acattcttgt tggtcagccg acctgagtgg gggctgatgc
    20281 ccggctggtt aatggcaggt ggtctgatcg cctggtttgt cggttggcgc aaaacacgct
    20341 gattttttca tcgctcaagg cgggccgtgt aacgtataat gcggctttgt ttaatcatca
    20401 tctaccacag aggaacatgt atgggtggta tcagtatttg gcagttattg attattgccg
    20461 tcatcgttgt actgcttttt ggcaccaaaa agctcggctc catcggttcc gatcttggtg
    20521 cgtcgatcaa aggctttaaa aaagcaatga gcgatgatga accaaagcag gataaaacca
    20581 gtcaggatgc tgattttact gcgaaaacta tcgccgataa gcaggcggat acgaatcagg
    20641 aacaggctaa aacagaagac gcgaagcgcc acgataaaga gcaggtgtaa tccgtgtttg
    20701 atatcggttt tagcgaactg ctattggtgt tcatcatcgg cctcgtcgtt ctggggccgc
    20761 aacgactgcc tgtggcggta aaaacggtag cgggctggat tcgcgcgttg cgttcactgg
    20821 cgacaacggt gcagaacgaa ctgacccagg agttaaaact ccaggagttt caggacagtc
    20881 tgaaaaaggt tgaaaaggcg agcctcacta acctgacgcc cgaactgaaa gcgtcgatgg
    20941 atgaactacg ccaggccgcg gagtcgatga agcgttccta cgttgcaaac gatcctgaaa
    21001 aggcgagcga tgaagcgcac accatccata acccggtggt gaaagataat gaagctgcgc
    21061 atgagggcgt aacgcctgcc gctgcacaaa cgcaggccag ttcgccggaa cagaagccag
    21121 aaaccacgcc agagccggtg gtaaaacctg ctgcggacgc tgaaccgaaa accgctgcac
    21181 cttccccttc gtcgagtgat aaaccgtaaa catgtctgta gaagatactc aaccgcttat
    21241 cacgcatctg attgagctgc gtaagcgtct gctgaactgc attatcgcgg tgatcgtgat
    21301 attcctgtgt ctggtctatt tcgccaatga catctatcac ttggtatccg cgccattgat
    21361 caagcagttg ccgcaaggtt caacgatgat cgccaccgac gtggcctcgc cgttctttac
    21421 gccgatcaag ctgaccttta tggtgtcgct gattctgtca gcgccggtga ttctctatca
    21481 ggtgtgggca tttatcgccc cagcgctgta taagcatgaa cgtcgcctgg tggtgccgct
    21541 gctggtttcc agctctctgc tgttttatat cggcatggca ttcgcctact ttgtggtctt
    21601 tccgctggca tttggcttcc ttgccaatac cgcgccggaa ggggtgcagg tatccaccga
    21661 catcgccagc tatttaagct tcgttatggc gctgtttatg gcgtttggtg tctcctttga
    21721 agtgccggtg gcaattgtgc tgctgtgctg gatggggatt acctcgtcag aagacttacg
    21781 caaaaaacgc ccgtatgtgc tggttggtgc attcgttgtc gggatgttgc tgacgccgcc
    21841 ggatgtcttc tcgcaaacgc tattggcgat cccgatgtac tgtctgtttg aaatcggtgt
    21901 cttcttctca cgcttttacg ttggtaaagg gcgaaatcgg gaagaggaaa acgacgctga
    21961 agcagaaagc gaaaaaactg aagaataaat tcaaccgccc gtcagggcgg ttgtcatatg
    22021 gagtacagga tgtttgatat cggcgttaat ttgaccagtt cgcaatttgc gaaagaccgt
    22081 gatgatgttg tagcgcgcgc ttttgacgcg ggagttaatg ggctactcat caccggcact
    22141 aacctgcgtg aaagccagca ggcgcaaaag ctggcgcgtc agtattcgtc ctgttggtca
    22201 acggcgggcg tacatcctca cgacagcagc cagtggcaag ctgcgactga agaagcgatt
    22261 attgagctgg ccgcgcagcc agaagtggtg gcgattggtg aatgtggtct cgactttaac
    22321 cgtaactttt cgacgccgga agagcaggaa cgcgcttttg ttgctcagct acgcattgcc
    22381 gcagaattaa acatgccggt atttatgcac tgtcgcgatg cccacgagcg gtttatgaca
    22441 ttgctggagc cgtggctgga taaactgcct ggtgcggttc ttcattgctt taccggcaca
    22501 cgcgaagaga tgcaggcgtg cgtggcgcgt ggaatttata tcggcattac cggttgggtt
    22561 tgcgatgaac gacgcgggct ggagctgcgg gaattgttgc cgttgattcc ggcggagaaa
    22621 ttgctgatcg aaacagatgc gccgtatctg ctcccgcgcg atctcacgcc aaagccatca
    22681 tcccggcgca acgagccagc ccatctgccc catattttgc aacgtattgc gcactggcgt
    22741 ggagaagatg ccgcatggct ggctgccacc acggatgcca atgtcaaaac actgtttggg
    22801 attgcgtttt agagtttgcg gaactcggtg ttcttcacac tgtgcttaat ctctttatta
    22861 ataagattaa gcaatagcat ggagcgagcc tcaccatcgg gttcggtgaa aatggcctga
    22921 aagccttcga acgcgccttc ggtaataatc accttatcac ccggataagg gggggccgga
    22981 tcgacaatgt ctttcggttt atataccgat agctgatgaa ttaccgccga tgggactatc
    23041 gctggcgacg cgccaaagcg cacaaagtgg ctgacgccac gcgtagcgct gatggtcgtg
    23101 gtatgaatca cttctggatc aaattccaca aacaggtagt tagggaataa cggttcactg
    23161 actgcggtac gtttaccccg tacgattttt tccagggtga tcatcggagc gaggcaatta
    23221 accgcctgtc tctcaaggtg ttcctgggca cgttgaagtt gcccgcgctt gcagtacagt
    23281 aaataccagg attgcataat gactcttatc cgtttaatcg gggcgcaagg atagcaaaag
    23341 ctttacgcta agttaattat attccccggt ttgcgttata ccgtcagagt tcacgctaat
    23401 ttaacaaatt tacagcattg caaagatgaa cgccgtataa tgggcgcaga ttaagaggct
    23461 acaatggacg ccatgaaata taacgattta cgcgacttcc tgacgctgct tgaacagcag
    23521 ggtgagctaa aacgtatcac gctcccggtg gatccgcatc tggaaatcac tgaaattgct
    23581 gaccgcactc tgcgtgctgg tgggcctgcg ctgttgttcg aaaaccctaa aggctactca
    23641 atgccggtgc tgtgcaacct gttcggtacg ccaaagcgcg tggcgatggg catggggcag
    23701 gaagatgttt cggcgctgcg tgaagttggt aaattattgg cgtttctgaa agagccggag
    23761 ccgccaaaag gtttccgcga cctgtttgat aaactgccgc agtttaagca ggtgttaaac
    23821 atgccgacaa agcgactgcg tggtgcgccc tgccaacaaa aaatcgtctc tggcgatgac
    23881 gtcgatctca atcgcattcc cattatgacc tgctggccgg aagatgccgc gccgctgatt
    23941 acctgggggc tgaccgtaac gcgcggcccg cataaagagc ggcagaatct gggcatttat
    24001 cgccagcagc tgattggtaa aaacaaactg attatgcgct ggctgtcgca tcgcggcggc
    24061 gcgctggatt atcaggagtg gtgtgcggcg catccgggcg aacgtttccc ggtttctgtg
    24121 gcgctgggtg ccgatcctgc cacgattctc ggtgcagtca cccccgttcc ggatacgctt
    24181 tcagagtatg cgtttgccgg attgctacgc ggcaccaaaa ccgaagtagt aaagtgtatt
    24241 tccaatgacc tcgaagtgcc agccagtgcc gaaatcgtcc tggaagggta tatcgatcct
    24301 ggtgagatgg cgccggaagg gccgtatggt gaccacacag gttactataa tgaagtcgat
    24361 aatttcccgg tgtttaccgt gacgcatatt acccagcgtg aagatgcgat ttaccattcc
    24421 acctataccg ggcgtccgcc agatgagccc gcggtactgg gcgtggcgtt gaacgaagtg
    24481 tttgtaccga ttctgcaaaa acagttcccg gaaattgtcg atttttacct gccgccggaa
    24541 ggctgttctt atcgtctggc ggtagtgacg atcaaaaaac agtacgccgg acacgcgaag
    24601 cgcgtcatga tgggcgtctg gtcgttctta cgccagttta tgtacactaa atttgtgatc
    24661 gtttgcgatg atgacgtcaa cgcacgcgac tggaacgatg tgatttgggc gattaccacc
    24721 cgtatggacc cggcgcggga tactgttctg gtagaaaata cgcctattga ttatctggat
    24781 tttgcctcgc ctgtctctgg gctgggttca aaaatggggc tggatgccac gaataaatgg
    24841 ccgggggaaa cccagcgtga atggggacgt cccatcaaaa aagatccaga tgttgtcgcg
    24901 catattgacg ccatctggga tgaactggct atttttaaca acggtaaaag cgcctgatgc
    24961 gcgtttgttt tgccctattt atcgatccga cagagaaagc gcatgacaac cttaagctgt
    25021 aaagtgacct cggtagcagc tatcacggat accgtatatc gtgtccgcat cgtgccagac
    25081 gcggcctttt cttttcgtgc tggtcagtat ttgatggtag tgatggatga gcgcgacaaa
    25141 cgtccgttct caatggcctc gacgccggat gaaaaagggt ttatcgagct gcatattggc
    25201 gcttctgaaa tcaaccttta cgcgaaagca gtcatggacc gcatcctcaa agatcatcaa
    25261 atcgtggtcg accttcccca cggagaagcg tggctgcgcg atgatgaaga gcgtccgatg
    25321 attttgattg cgggcggcac cgggttctct tatgcccgct cgattttgct gacagcgttg
    25381 gcgcgtaacc caaaccgtga tatcaccatt tactggggcg ggcgtgaaga gcagcatctg
    25441 tatgatctct gcgagcttga ggcgctttcg ttaaagcacc ttggtctgca agtggtgccg
    25501 gtggttgaac aaccggaagc aggctggcgt gggcgtactg gtaccgtgtt aacggcggta
    25561 ttgcaggatc atggtacgct ggcagagcat gatatctata ttgccggacg ttttgagatg
    25621 gcgaaaattg cccgtgacct gttttgcagt gagcgtaatg cgcgggaaga tcgcctgttt
    25681 ggcgatgcgt ttgcatttat ctgagatata aaaaaacccg cccctgacag gcgggaagaa
    25741 cggcaactaa aactttattc agtggcattt agatctatgt cgcatccggc aattggtgca
    25801 cgatgcctga tgcgacgctg gcgcgtctta tcaggcctac attggtgccg gatcggtaga
    25861 ccggataagg cgttcacgcc gcatccggca agtggttaaa cccgctcaaa caccgtcgca
    25921 ataccctgac ccagaccgat acacatcgtc gccagaccaa actgaacgtc tttgcgttcc
    25981 atcagattca gcagcgtggt gctgatacgc gcaccggaac aacccagcgg atgacccagc
    26041 gcgatcgcac caccattgag gttgatcttc tcgtcaatct gctccattag cccgagatct
    26101 ttaatgcacg gcaggatctg cgcggcaaag gcttcgttca tctcaaacac gccgatatcg
    26161 ctggcagaaa gccccgcttt tttcagcgcc agcttcgagg ccggaaccgg gccgtaaccc
    26221 ataatcgatg ggtcacaacc aacgaccgcc atcgaacgca cacgagcgcg cggcttaaga
    26281 cctaattcat gggcgcggct ttcactcatc accagcatgg cagctgcgcc atcggaaagt
    26341 gcagaagatg tgcccgccgt taccgtaccg tttactggat caaacgccgg acgcagcgtg
    26401 gcgagggctt ccacggtggt ttccgggcga atcacttcgt cgtaattaaa ctgcttcagg
    26461 acgccgtcgg catcgtgacc accagttggg atgatttcat ttttaaatgc ggccgactgc
    26521 gtggcggccc aggcgcgggc gtgtgaccgc gcggcaaagg catcctgcat ttcacggctg
    26581 ataccgtgca tacgcgccag catttctgcc gttaagccca tcatgcccgc cgctttggcg
    26641 acattgcggc tcaggccggg gtgaaaatcg acgccgtgac tcatcggcac atggcctata
    26701 tgctccacgc cgccaaccag acatgcctgc gtatcgccag tcatgatcat tcgtgctgcg
    26761 tcatgcagtg cctgcatgga tgaaccacac aagcgattga cggtaaccgc cgggacagag
    26821 tgcggcactt ctgccagcag cgccgcgtta cgggcgatgt taaaaccctg ctccagcgtc
    26881 tgttgcacac aaccccagta aatatcgtcg agggccgccg cttccagcgc cgggttacgc
    26941 gccagcaggc tacgcattaa atgagcggag agatcttccg cacgcacgtt acgaaaagca
    27001 ccgcccttcg aacggcccat cggggtgcga attgcatcga caatgacaac ctgttccatt
    27061 gtgactcctt aagccgtttt caggtcgcca accggacggg ctggctcaac cggaggatag
    27121 tacggttcgt tatgacgcgc tttattacgc agaccttccg gcacttcata cagcgggccg
    27181 aggtgctgat attgctgtgc catatcgagg tattttgcgc taccgagggt gtccagccag
    27241 cggaacgcgc cgccgtggaa cggagggaag cccaggccgt agaccagcgc catatccgct
    27301 tccgccggag tggcgataat gccctcttcc agacagcgca ccacttcgtt gaccatcggg
    27361 atcatcatgc gggcgataat ctcttcttcg ctgaaatcgc gcttcggttg gctcacttct
    27421 gccagcaggt cttcaacggc ggcgtcttct tctttcttcg gcttaccttt gctgtcttct
    27481 ttataacgcc agaaaccgag gccgttcttc tgaccaaagc ggttggcatc aaacagcgcg
    27541 tcgatggcat cgcggtaatc tttctgcatc cgctgcggga agcctgctgc catgacagcc
    27601 tgagcgtgat gcgcggtatc aatgcccaca acgtccagca gatatgccgg gcccatcggc
    27661 cagccaaact gtttttccat cactttgtcg atcttgcgga aatccgcgcc gtcgcgcagc
    27721 agctggctga aaccggcgaa atacgggaac agcacgcggt taacaaagaa gccggggcag
    27781 tcgttaacca caatcggcgt cttgcccatc ttgctcgccc aggcgacaac tttcgcgatg
    27841 gtttcgtcgg agcttttctc gccgcgaata atttctacca acggcattcg gtggaccggg
    27901 ttaaagaagt gcatcccgca gaagttttcc gggcgttcca gcgcgttggc cagttcgctg
    27961 ataggaatgg ttgaagtgtt agacgccagc acggtatcct ggcgtacttt ttgttcggtt
    28021 tctgccagta cggctttttt cactttcggg ttttcaacaa ccgcttctac cacaatatcc
    28081 acgcggtcaa atccggcgta gtcgagcgtt gggtggatcg tggagatcac gccagccagt
    28141 ttcagaccat cgattttgcc gcgctcaagc tgcttgttca gcagtttcgc ggcttcggtc
    28201 atgccgaggg ttaacgactt gtcgttgata tctttcatga caaccggcac gcctttccac
    28261 gcggactggt aagcgatacc gccacccatg atgcctgcgc ccagcacagc agcctgtttc
    28321 ggggtttcaa cgtctttggt gagtttcttc gctttgcctt ttacatattg atcgttaagg
    28381 aaaatgccga ccagtgcgcg agcttcgttg gtatgcgcca gcgggacaaa acttttgttt
    28441 tccagattta aggcttcttc acgaccaaaa cgggccgcag cttcaatggt ttttactgcg
    28501 gtgatggggg ccggataatg tttccccgcc gtttgcgcga ccattccttt agcgatggtg
    28561 aagctcatgg tggcttcaat cttgctcagt tttagcggtt cctgcttcgg ctgacgtttc
    28621 gctttccagt cgaggtcgcc gttaatggcc tggcgtaaaa tcgccatcgc cccttcaatc
    28681 agtttttctg ctttgactac gccatccacc agaccgattt tcagcgcctg atccgcgccg
    28741 acatctttac cggcggcaat gatttccagt gcactgtcag cgcccagcat acgtggcata
    28801 cgtacagaac cgccaaagcc cggcatgatg cccagtttgg tttccggcag accgatgcgc
    28861 agatccggcg tcgccagacg ataatcggtc gccagcacgc attcgcagcc accacccagc
    28921 gcatagccgt tgacggcagc aatggtcggc accggcagat cttccaggcg attaaacacg
    28981 ctattggcaa agtgcagcca ctgacttaac tgttcttcag gaacgaggaa cagggacaaa
    29041 aattcggtga tatcagcacc gacgataaag gctgctttgt tcgaacgcag cagcagccct
    29101 tttagatctg attgctgttc cagcacgccg atggcctcgc cgaggctggc gacggtcgca
    29161 gtgtcgagtt tattaactga acctggggca tcaaatacca gttcggcaat gccatcttcc
    29221 agccagtcaa ggtacagggt gtcgcctttg taaagcatgt cagtctcctg aatccgcaag
    29281 gtaatctggt cgtaccagat gcatcggagt gtgtgttttg tgttaaaaat atgcaaataa
    29341 aagattaaag aaatgccgat ctgatcacgc tcggcagaaa tcacgctctg gatgaacgat
    29401 gtgctaagat gcggagactt aaggtcaaaa aaacagaagg gtaaaaaatg gaatcactgg
    29461 cctcgctcta taaaaatcat atagctacct tacaagaacg gactcgcgat gcgctggcgc
    29521 gcttcaagct ggatgcatta cttattcact ccggcgagct gttcaacgtt tttctcgacg
    29581 atcatcccta tccgtttaaa gtgaacccgc aattcaaagc gtgggtgccg gtaactcagg
    29641 tgccaaactg ctggttgctg gtggatggcg tgaacaagcc gaaactgtgg ttctatctgc
    29701 cggttgatta ctggcacaac gtcgaaccgc tgccgacctc cttctggact gaagatgtgg
    29761 aagtgatcgc gctgccgaaa gccgatggca ttggtagcct gctgcctgct gcgcgcggca
    29821 atatcggtta tatcggtccg gtgccggaac gtgcgctgca actgggtatt gaggccagca
    29881 acatcaaccc gaaaggggtg atcgactacc tgcattatta ccgctccttc aaaaccgagt
    29941 acgaactggc ctgtatgcgt gaagcgcaga aaatggcggt caatggtcat cgtgcggcag
    30001 aagaagcgtt ccgttctggc atgagcgagt tcgatatcaa tattgcctat ctgaccgcga
    30061 ccggtcatcg tgataccgac gtaccttaca gcaacattgt ggcgcttaac gaacacgctg
    30121 cggtgttgca ttacaccaaa ctggaccacc aggcaccgga agagatgcgc agcttcctgc
    30181 tggatgccgg ggccgaatat aacggctatg cggctgacct gacccgtacc tggtcggcaa
    30241 aaagcgacaa cgactacgca cagctggtga aagacgtaaa tgatgaacaa ctggcgctga
    30301 tcgccaccat gaaagcaggc gtcagctatg tggattacca catccagttc catcagcgca
    30361 tcgccaaact gctgcgtaaa catcaaatca tcaccgatat gagtgaagaa gcgatggtcg
    30421 aaaacgatct caccggaccg tttatgccgc acggtattgg ccatccgctg ggcctgcagg
    30481 tgcatgacgt cgccggtttt atgcaggatg atagcggtac acacctcgcg gcaccggcaa
    30541 aatatccgta cctgcgctac acccgtattc tccagccggg catggtgtta accatcgaac
    30601 cgggtatcta cttcattgaa tcgcttctgg caccgtggcg tgaagggcag ttcagcaagc
    30661 acttcaactg gcagaaaatt gaagcactga aaccgttcgg cggcattcgt atcgaagaca
    30721 acgtggtgat ccacgaaaac aacgtggaaa acatgacccg ggatctgaaa ctggcgtgat
    30781 ggaaagctgg ttaattcctg cggcaccggt cacggtcgtt gaagagatca aaaagagccg
    30841 cttcataacg atgttggcgc ataccgatgg cgttgaggcg gcgaaagcgt ttgttgaatc
    30901 ggtgcgggca gaacaccccg atgcccgcca ccattgcgtg gcgtgggtgg cgggtgcgcc
    30961 ggatgattct caacagctgg gtttctctga cgacggggag ccggcgggaa cggcaggtaa
    31021 accgatgctc gcccagctaa tgggcagcgg cgtcggggaa attaccgctg tggtagtgcg
    31081 ctactacggc ggcatattgc ttggcaccgg tgggttagtg aaagcgtatg gcggcggcgt
    31141 gaatcaggcg ctgcgccagc taacgaccca acgcaagacg ccattaaccg aatatacttt
    31201 gcaatgtgaa tatagtcagt taaccggcat tgaagcgttg ctggggcagt gtgacggcaa
    31261 aattatcaac agtgattatc aggcattcgt tctgctgcgg gtggcgcttc cggcggcgaa
    31321 agtggctgaa ttttccgcaa agctggcgga ttttagccgt ggttcattgc aattgttagc
    31381 gattgaagaa taatccccac ttcgttttgc agaactaagg aagcggcaga gatgcatttt
    31441 cgcgccatta cccgaatcgt tggactactg gtcatcttat tttcagggac catgattatc
    31501 cctgggctgg tagcactcat ctaccgggat ggagcgggcc gcgcttttac ccagaccttt
    31561 tttgtcgccc tcgccattgg ctctatgctg tggtggccga accgcaaaga gaagggcgaa
    31621 ctgaaatccc gtgaggggtt tctgatagtg gtgctgtttt ggaccgtgct gggtagtgtc
    31681 ggtgcgctcc cttttatctt ctcggaaagc ccgaacctca cgattaccga tgcgtttttt
    31741 gaatctttct ctggcctgac caccaccggg gccactacgc tggtggggct ggattcgctc
    31801 cctcatgcca tcctctttta tcgccagatg ctgcaatggt ttggcgggat ggggatcatc
    31861 gtgttagcgg ttgcgatact gcctatcctc ggcgtgggtg ggatgcagct ctatcgcgca
    31921 gaaatgcccg gcccgctgaa agataacaaa atgcgcccgc gaattgcgga aacggcgaaa
    31981 actctgtggt tgatttatgt cttgctgacc gtcgcctgtg cgctggcgtt gtggtttgcc
    32041 ggaatggatg cctttgatgc catcggccat agctttgcga ctatcgctat tggcggcttc
    32101 tcgacacatg atgccagtat cggttatttc gacagtccga ctattaacac tatcattgct
    32161 atcttcctgc tgatctccgg ctgtaactac ggtctgcact tttcactgtt aagtgggcgt
    32221 agtctgaagg tttattggcg cgatccggaa tttcgcatgt ttatcggcgt acagtttacg
    32281 ctggtggtta tttgtacact cgtactgtgg tttcataatg tctacagttc ggcgctgatg
    32341 acaattaacc aggcgttttt ccaggtggta tcgatggcga caaccgccgg gtttacgact
    32401 gacagcattg cccgctggcc gctctttttg ccggtactgc ttttatgttc agcttttatc
    32461 ggcggttgcg ccgggtcaac gggcggtggc ctgaaagtga tccgcatcct gctgctgttt
    32521 aagcagggga accgtgagct gaaacgactg gtgcatccga acgccgtcta tagcattaag
    32581 ctggggaatc gcgcactgcc ggaacgtatc ctcgaagccg tttggggatt tttctccgcc
    32641 tatgcattgg tgtttattgt cagtatgctg gcgattatcg ccacgggcgt ggatgatttt
    32701 tctgcctttg cctcagttgt tgcgacattg aataacctgg ggccagggct tggcgtggtt
    32761 gctgataact ttaccagtat gaacccggtg gctaaatgga tcctgattgc caacatgctg
    32821 tttggtcgtc tcgaggtctt tacattactg gtgctcttta ccccgacttt ctggcgtgaa
    32881 tgatggagta atacgtgaaa acattaattc ttttctcaac aagggacgga caaacgcgcg
    32941 agattgcctc ctacctggct tcggaactga aagaactggg gatccagacg gatgtcgcca
    33001 atgtgcaccg cattgaagaa ccacaatggg aaaactatga ccgtgtggtc attggtgctt
    33061 ctattcgcta tggtcactac cactctgcgt tccaggaatt tgtcaaaaaa catgcgacgc
    33121 gactgaattc gatgccgagc gccttttatt ccgtaaacct ggtggcgcgc aaaccggaga
    33181 agcgtactcc acagaccaac agctacgcgc ggaagtttct gatgaactcg caatggcgtc
    33241 ccgatcgctg cgcggtcatt gccggggcgc tgcgttaccc acgttatcgc tggtacgacc
    33301 gttttatgct caagctgatt atgaagatgt caggcggtga aacggatacg cgcaaagaag
    33361 ttgtctatac cgattgggag caggtggcga atttcgcccg agaaatcgcc catttaaccg
    33421 acaaaccgac gctgaaataa gcataaagaa taaaaaatgc gcggtcagaa aattatttta
    33481 aatttcctct tgtcaggccg gaataactcc ctataatgcg ccaccactga cacggaacaa
    33541 cggcaaacac gcagccgggt cagcggggtt ctcctgagaa ttccgacaga gaaagcaaaa
    33601 ataaatgctt gactttgtag cgggaaagcg tattatgaac accctgcgc
//
LOCUS       DAJTUI010000037        33511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTUI010000037 DAJTUI010000000
VERSION     DAJTUI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.037"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="OO464_001908"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="OO464_001908"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR6227073.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEECVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="OO464_001909"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="OO464_001909"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6227074.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO464_001910"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO464_001910"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6227075.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO464_001911"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO464_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6227076.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO464_001912"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO464_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6227077.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO464_001913"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO464_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR6227078.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="OO464_001914"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="OO464_001914"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6227079.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="OO464_001915"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="OO464_001915"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6227080.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="OO464_001916"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="OO464_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6227081.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO464_001917"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO464_001917"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6227082.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO464_001918"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO464_001918"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6227083.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="OO464_001919"
     CDS             8212..9075
                     /locus_tag="OO464_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6227084.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="OO464_001920"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="OO464_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6227085.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="OO464_001921"
     CDS             10411..11028
                     /locus_tag="OO464_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6227086.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO464_001922"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO464_001922"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6227087.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="OO464_001923"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="OO464_001923"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6227088.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO464_001924"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO464_001924"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6227089.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="OO464_001925"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="OO464_001925"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6227090.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="OO464_001926"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="OO464_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6227091.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="OO464_001927"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="OO464_001927"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6227092.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO464_001928"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO464_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6227093.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="OO464_001929"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="OO464_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6227094.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="OO464_001930"
     CDS             21859..23568
                     /locus_tag="OO464_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6227095.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO464_001931"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO464_001931"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6227096.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="OO464_001932"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="OO464_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="OO464_001933"
     tRNA            27625..27719
                     /locus_tag="OO464_001933"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="OO464_001934"
     CDS             27991..29202
                     /locus_tag="OO464_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6227097.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29614..30657
                     /locus_tag="OO464_001935"
     CDS             29614..30657
                     /locus_tag="OO464_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6227098.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31172..31600
                     /locus_tag="OO464_001936"
                     /pseudo
     CDS             31172..31600
                     /locus_tag="OO464_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31630..32781)
                     /locus_tag="OO464_001937"
                     /pseudo
     CDS             complement(31630..32781)
                     /locus_tag="OO464_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32846..33494
                     /locus_tag="OO464_001938"
                     /pseudo
     CDS             32846..33494
                     /locus_tag="OO464_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8396 a         8808 c         8258 g         8049 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagagt gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaatacag ccttgcagtt atttcgatac ctttagcaaa
    29401 ggaatagttg attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg
    29461 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
    29521 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
    29581 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
    29641 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
    29701 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
    29761 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
    29821 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
    29881 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
    29941 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
    30001 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
    30061 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
    30121 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
    30181 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
    30241 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
    30301 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
    30361 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
    30421 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
    30481 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
    30541 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
    30601 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
    30661 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
    30721 cttccccagg tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
    30781 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
    30841 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
    30901 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
    30961 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
    31021 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
    31081 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
    31141 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
    31201 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
    31261 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
    31321 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
    31381 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
    31441 tgttacaccg atagtgacgc cagccttgct aagccgtgag ggttttttat atccgacgcc
    31501 agcgacctcc attcccagag acctgcttga acctttgcca aaaggagcat tttcgattgg
    31561 tgagattggg cagtatgaca aatacaagac gacccatact gatcctaccc acgtaatgtg
    31621 gacacggccc taagcgagat tatggttttc aaattgttcc gggctgagac cgccacaggc
    31681 actgtgacga cgccagcgat tgtaatcaca ctcgatataa ttaaacaccg ttgcccgcat
    31741 tatttcccgg ctgctaaagc gttccccgtg gatacattcc accttcagcg aatgaaagaa
    31801 gctttccaca caggcattgt cataacagta gcctttcgca ctcatactgc cacgcaggtt
    31861 gtgtcgcttc agcattgcct gataatcccc tgaacagtat tgaccaccac ggtccgtatg
    31921 aacaatgacg ctttccgggc gtcttctccg ccacaacgcc atttgcagtg catcacaggc
    31981 cagttgtgct gtcattcgcg gtgacatcga ccagccaatg acggcgcgtg accacaggtc
    32041 gatgactact gcgagataca accagccctc atcggtacgc aagtacgtga tgtcacccgc
    32101 ccacttctgg tttcgggcca ctggcgctga agtcctgctc cagcagattc tccaatacgg
    32161 gcaggccatg tgcacggtag ctgaccgggc tgaacttccg gctggctttc gcccgcagcc
    32221 cctgacgacg caggctggcg gcaatggttt taatattgaa ctccggcagt tcgtcagcaa
    32281 ggcggggagc accgtatcgc tgctttgcct caaagaatgc cttatggaca gcggcatcgc
    32341 aggtgagccg aaactgttgg cgcaggctca tctggtgacg acgcctgagc cagacatacc
    32401 agccgctgcg ggcaacccga agtacacgac acatcgcttt gatgctgaac tctgcccgat
    32461 gattttcgat aaagacatac ttcatttcag gcgcttcgcg aagtatgtcg cggccttttg
    32521 gaggatggac agttcctcag cctgctccgc cagttgtcgt ttaaggcgga cattttcagc
    32581 ggccagttcg ctttcgcgct ctgacgaact catttgttgc tgctgtttac tgcgccaggc
    32641 ataaagctga gattcataca ggctgagttc acgggctgcg gcggccacac cgatgcgttc
    32701 agcgagtttc agggcttcgt tacgaaattc aggcgtatgt tgtttacggg gcttcttgct
    32761 gattgatact ggttttgtca tgagtcacct ctggttgaga gtttactcac ttagtcctgt
    32821 gtccactatt ggtgggtaag atcaccctcc attaaaacta aaacgggcgc tttctgatat
    32881 atcatctgta agcgcagtaa taccattagt agcacctcgc gcattgatta ttgaatatta
    32941 taactggaat gttatatttc ttgttatgat gctgttcagt attataataa tgttattatc
    33001 accaaagata actcatgcaa atgagaaagt aatatgtcaa aacagtgcat cctttgtgac
    33061 caaagagttt atctgcggca ctatacttgt ctcattaata ctcagttctc tattttgcta
    33121 catatcaata tcaccatcaa ttttcatgca tacttatggc ctgagtactt ttagttactc
    33181 aattattttc tccttctcag ttttattttt cataatcgga aatcaattat caaagattga
    33241 aaaattatcc aacctatggt ttttattacc caatagccaa ttttatcgca ttacaaataa
    33301 atggttcaaa aacaggattt gccgcatcta ttacaggttt tactcaaaca ataagcgctg
    33361 gtacaatcag tttcctgtca gtaaccgtaa gcgacaacgg tgttggtccc ggaactactt
    33421 taggtcttgg ttcactaata acttcattac tgtcaatagc cgtaatcgcc attgcaggaa
    33481 ataataaaaa gtaagtgtac tctgactgcg t
//
LOCUS       DAJTUI010000038        33448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTUI010000038 DAJTUI010000000
VERSION     DAJTUI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.038"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            90..1586
                     /gene="ravA"
                     /locus_tag="OO464_001939"
     CDS             90..1586
                     /gene="ravA"
                     /locus_tag="OO464_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6227099.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1580..3031
                     /gene="viaA"
                     /locus_tag="OO464_001940"
     CDS             1580..3031
                     /gene="viaA"
                     /locus_tag="OO464_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR6227100.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3036..4028)
                     /gene="asnA"
                     /locus_tag="OO464_001941"
     CDS             complement(3036..4028)
                     /gene="asnA"
                     /locus_tag="OO464_001941"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6227101.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4180..4638
                     /gene="asnC"
                     /locus_tag="OO464_001942"
     CDS             4180..4638
                     /gene="asnC"
                     /locus_tag="OO464_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6227102.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4728..5171
                     /gene="mioC"
                     /locus_tag="OO464_001943"
     CDS             4728..5171
                     /gene="mioC"
                     /locus_tag="OO464_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6227103.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5550..7439
                     /gene="mnmG"
                     /locus_tag="OO464_001944"
     CDS             5550..7439
                     /gene="mnmG"
                     /locus_tag="OO464_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6227104.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7503..8126
                     /gene="rsmG"
                     /locus_tag="OO464_001945"
     CDS             7503..8126
                     /gene="rsmG"
                     /locus_tag="OO464_001945"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6227105.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8743..9123
                     /gene="atpI"
                     /locus_tag="OO464_001946"
     CDS             8743..9123
                     /gene="atpI"
                     /locus_tag="OO464_001946"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6227106.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9132..9947
                     /gene="atpB"
                     /locus_tag="OO464_001947"
     CDS             9132..9947
                     /gene="atpB"
                     /locus_tag="OO464_001947"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6227107.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9994..10233
                     /gene="atpE"
                     /locus_tag="OO464_001948"
     CDS             9994..10233
                     /gene="atpE"
                     /locus_tag="OO464_001948"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6227108.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10295..10765
                     /gene="atpF"
                     /locus_tag="OO464_001949"
     CDS             10295..10765
                     /gene="atpF"
                     /locus_tag="OO464_001949"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6227109.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10780..11313
                     /gene="atpH"
                     /locus_tag="OO464_001950"
     CDS             10780..11313
                     /gene="atpH"
                     /locus_tag="OO464_001950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6227110.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11326..12867
                     /gene="atpA"
                     /locus_tag="OO464_001951"
     CDS             11326..12867
                     /gene="atpA"
                     /locus_tag="OO464_001951"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6227111.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12918..13781
                     /gene="atpG"
                     /locus_tag="OO464_001952"
     CDS             12918..13781
                     /gene="atpG"
                     /locus_tag="OO464_001952"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6227112.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13808..15190
                     /gene="atpD"
                     /locus_tag="OO464_001953"
     CDS             13808..15190
                     /gene="atpD"
                     /locus_tag="OO464_001953"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6227113.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15211..15630
                     /gene="atpC"
                     /locus_tag="OO464_001954"
     CDS             15211..15630
                     /gene="atpC"
                     /locus_tag="OO464_001954"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6227114.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15981..17351
                     /gene="glmU"
                     /locus_tag="OO464_001955"
     CDS             15981..17351
                     /gene="glmU"
                     /locus_tag="OO464_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6227115.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17513..19342
                     /gene="glmS"
                     /locus_tag="OO464_001956"
     CDS             17513..19342
                     /gene="glmS"
                     /locus_tag="OO464_001956"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6227116.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19504..20325
                     /locus_tag="OO464_001957"
     CDS             19504..20325
                     /locus_tag="OO464_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR6227117.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20312..22420
                     /locus_tag="OO464_001958"
     CDS             20312..22420
                     /locus_tag="OO464_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6227118.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22417..24084
                     /locus_tag="OO464_001959"
     CDS             22417..24084
                     /locus_tag="OO464_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6227119.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            24087..25613
                     /locus_tag="OO464_001960"
     CDS             24087..25613
                     /locus_tag="OO464_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR6227120.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25614..27230
                     /locus_tag="OO464_001961"
     CDS             25614..27230
                     /locus_tag="OO464_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6227121.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27286..27438)
                     /locus_tag="OO464_001962"
                     /pseudo
     CDS             complement(27286..27438)
                     /locus_tag="OO464_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27461..27838)
                     /locus_tag="OO464_001963"
     CDS             complement(27461..27838)
                     /locus_tag="OO464_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227122.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(28248..28619)
                     /locus_tag="OO464_001964"
     CDS             complement(28248..28619)
                     /locus_tag="OO464_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227123.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(28680..29177)
                     /locus_tag="OO464_001965"
     CDS             complement(28680..29177)
                     /locus_tag="OO464_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227124.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(29253..30041)
                     /gene="aadA1"
                     /locus_tag="OO464_001966"
     CDS             complement(29253..30041)
                     /gene="aadA1"
                     /locus_tag="OO464_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6227125.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(30099..30623)
                     /gene="sat2"
                     /locus_tag="OO464_001967"
     CDS             complement(30099..30623)
                     /gene="sat2"
                     /locus_tag="OO464_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6227126.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(30718..31191)
                     /locus_tag="OO464_001968"
     CDS             complement(30718..31191)
                     /locus_tag="OO464_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6227127.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            31523..32059
                     /locus_tag="OO464_001969"
     CDS             31523..32059
                     /locus_tag="OO464_001969"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR6227128.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            32174..32500
                     /locus_tag="OO464_001970"
     CDS             32174..32500
                     /locus_tag="OO464_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6227129.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(32688..32927)
                     /locus_tag="OO464_001971"
     CDS             complement(32688..32927)
                     /locus_tag="OO464_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR6227130.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         8673 a         7807 c         8449 g         8519 t
ORIGIN      
        1 cccccctttg agactatttt agtcaccagc aagaaacgtc tatactcgca atttacgcag
       61 aacttttgac gaaaggacgc cacttcatta tggctcaccc tcatttatta gcggaaagaa
      121 tttcccgcct gagcagttcg ttggaaaagg ggctttatga acgtagccac gccatccgct
      181 tgtgtttatt agcggcatta agtggtgaaa gtgtgttcct ccttggcccg ccaggtattg
      241 ccaaaagttt gatcgcccgg cgcttaaaat tcgcctttca gaatgcccgc gcgtttgaat
      301 atctgatgac ccgcttctcc acgccggaag aagtttttgg tcccctttct attcaggcgc
      361 taaaagatga agggcgctat gaacgtttaa ccagcggtta cctgccggaa gcagaaatcg
      421 tctttctgga tgagatctgg aaagcgggcc cggcaattct taataccttg ctcaccgcca
      481 ttaacgagcg ccagttccgc aacggtgcac acgtagaaaa aatcccgatg cgtctactgg
      541 tggcggcctc caacgagctg ccggaagcag acagcagtct ggaagcgtta tatgaccgca
      601 tgctgattcg tctatggtta gataaagtgc aagataaagc gaatttccgc tccatgctga
      661 ccagtcaaca ggatgaaaac gacaatccgg ttcctgatgc cttgcaggtc acagatgaag
      721 aatatgaacg ctggcagaaa gagattggtg aaattacgct gcccgatcat gtatttgagc
      781 tgatttttat tctgcgccag caactggata aattaccgga tgcgccttat gtctcggatc
      841 gtcgctggaa aaaagcgatc cgcttattgc aggccagcgc cttttttagc ggtcgcagtg
      901 ctgttgcccc ggttgatctc attttgctga aagattgcct gtggtatgac gcgcaaagcc
      961 tgaatttgat acaacaacaa attgatgtat tgatgaccgg tcatgcctgg caacagcaag
     1021 ggatgttgac ccgcctgggc gcgattgtgc aacgtcacct gcaactccag cagcaacaaa
     1081 gcgataaaac agccttaacg ttaattcgtc tgggcggcat tttcagccgt cgtcagcagt
     1141 atcaacttcc tgttaacgtt actgcttcca ctctgactct gctgctgcaa aaaccgttaa
     1201 aactgcatga tatggaagtg gttcatatct cctttgagcg tagcgcgctg gaacagtggc
     1261 tgagtaaagg tggtgaaatt cgcggcaaac tgaacggtat cggctttgcc cagaaactga
     1321 atctggaagt tgatagcacc caacatcttg ttgtacgcga tgtaagttta caaggcagta
     1381 cgctggcact tcccggttca tcggctgaag gtctgccagg tgaaataaaa caacaactgg
     1441 aagagcttga aagcgactgg cgtaagcaac acgctttatt cagcgaacag caaaaatgtc
     1501 tgtttatccc tggcgactgg ttaggtcgca ttgaagacag cctacaggat gtcggtgcac
     1561 agattcgtca ggcacaacaa tgctaacgct ggatacgctt aatgtgatgc tggccgtcag
     1621 cgaagaggga ttgatcgaag agatgatcat cgcactgctg gcctcaccgc agctggcggt
     1681 cttctttgaa aaattcccac gactgaaggc ggcaatcact gatgatgttc cccgctggcg
     1741 tgaggcgctg cgcagtcggc tgaaagatgc ccgagtcccg ccggaactca ccgaagaggt
     1801 gatgtgctat cagcaaagcc agctcctctc cacgccgcag tttattgtgc agctaccaca
     1861 gatcctggac ttactgcatc gtctgaattc cccatgggca gaacaagccc ggcagttggt
     1921 tgatgctaac agcacaataa cttcagcgtt acacacgctt tttctccagc gttggcgttt
     1981 aagtctgatc gtgcaagcaa cgacgttaaa tcaacagcta ttagaagaag aacgcgaaca
     2041 actgttaagt gaagttcagg aacgcatgac gctgagcgga caacttgaac cgattctcgc
     2101 agataacaat accgcagctg gtcgtctgtg ggatatgagc gccggtcaac ttaaacgtgg
     2161 cgactatcag tttattgtga aatacggtga atttcttaac gaacagccgg aactgaaacg
     2221 cctggcagag cagctggggc gttctcggga agccaaatca ataccgcgca acgatgcgca
     2281 gatggaaacc ttccgcacca tggtgcgcga accggcgacg gttcctgagc aggttgatgg
     2341 tctgcaacaa agcgatgata ttttacgtct cctgccgcca gaactggcga cactagggat
     2401 aacggaactg gagtatgagt tttaccgtcg gctggtggaa aaacagttgc tcacctatcg
     2461 cctgcacggt gagtcgtggc gtgaaaaagt gttcgaacgt ccggtggtac ataaagatta
     2521 cgatgaacag ccgcgcgggc cgtttattgt ctgtgtggat acttccggct caatgggcgg
     2581 ctttaatgaa cagtgtgcga aagcgttctg cctggctttg atgcgcattg ctctcgctga
     2641 aaaccggcgc tgttatatta tgctattttc caccgagatc gtccgttatg agctttcagg
     2701 cccacaaggc atcgaacaag caatccgttt tttaagccag cagtttcgtg gcggcaccga
     2761 tcttgccagt tgttttcgcg ccattatgga acgcttgcaa agcagggaat ggtttgatgc
     2821 cgatgcggtg gtgatttctg attttatcgc tcagcggttg cctgacgacg tgacgagtaa
     2881 agtgaaagag ctgcagcggg tacatcagca tcgctttcat gccgtggcga tgtcggcaca
     2941 cggcaaaccc ggcatcatgc gcattttcga tcatatctgg cgctttgata ccgggatgcg
     3001 aagccgcctg ctcagacgct ggcggcgata aattattaca gcagagaagg gacgctctcg
     3061 cgaacagcag ctggccatac tccacactga acctggccga tatgcggcag ttgcagcagc
     3121 agcatagtca aacgagactg gccgataccg ccgccgatgg tctgcggcat ttcaccgcgc
     3181 agcagcgcct gatgccattc cagctgcaga cgatcttcat caccggtcag cgccagctga
     3241 tgcttcagcg tgtcggcatc cacgcggatc cccatggaag aaagctcaaa cgcatcttcc
     3301 agtaccgggt tccacaccag aatatcgccg ttcaggcccg catggcccag ctctgacggg
     3361 gtgctccagt catcataatc cggtgcgcgc acgtcgtgac gatgaccatc actcaacttg
     3421 ccgccaatcc cgacgaggaa taccgcgcca agatctttcg caatcgcccg ctcacgccct
     3481 ttggcatcga gatccggata acgagacagt aactcctggc tgtgtacgaa gtgaatctga
     3541 tccggcagga acggtgccag gccaaactct tcgttaaccg cagcttcggt tgctttaatt
     3601 cccgcccaga tcgcctctac cgtgcttttc agagtcgaga gttgacgctc accgtcgccc
     3661 attacgcgtt cccagtccca ctggtcaaca tagaccgagt gcaacggaga aagacggtct
     3721 tcatcggggc gaagggcttt catgtgcgtg tacagccctt cgcccgcgct gaagtcgtgt
     3781 tgccctaagg tctgacgttt ccacttcgcc agtgaatgaa ccacttcgaa ctgggcatca
     3841 ggcagagctt tcacttttac ctgcaccgct ttttcacagc ccgacaagtt atcctgcgtg
     3901 ccatccccca cacggctaag aatcggcgcc tggacttcga tcagccccag acgttcttcc
     3961 agttggcgag aaaagtgaga tttcacgaag ctaatttggc gttgtttggc aatgtaagcg
     4021 gttttcattt tttatactcc tgcgtcctgt tgcttatgat taagcaacaa aaaccgacac
     4081 acatgcaata atcattcaat aaaaagccta acacactttt gattcattaa aaaagacggc
     4141 taaaatagaa tgaatcatca atctgcataa gaaaatccta tggaaaatta tctgatcgac
     4201 aatctggacc gtggcatcct ggaagcatta atgggcaatg cgcgcaccgc ttacgccgaa
     4261 ctggcgaaac aatttggcgt cagtccgggg acgattcacg ttcgagtaga gaaaatgaag
     4321 caggcgggga tcattaccgg ggcgcgtatt gatgtcagcc cgaagcagct cggttatgac
     4381 gtaggctgct ttatcggcat tatattaaag agcgccaaag actacccttc cgcgctggca
     4441 aagctggaaa gccttgatga agtcactgaa gcctattaca caaccggcca ctacagcatc
     4501 tttataaaag ttatgtgccg ttcgatcgac gctctccagc atgtacttat caacaagatc
     4561 caaacaattg atgaaattca gtccaccgag acattgatcg tcctgcaaaa cccgatcatg
     4621 cgtaccatca agccctgatc ggctttttta atcccatact tttccacagg tagatcccaa
     4681 cgcgttcaca gcgtacaata cgccactctt aataaaggtg gcggtttatg gcagatatca
     4741 ctcttatcag cggcagcacc ctcggcggtg ccgaatatgt agcagaacac ctggctgaaa
     4801 agctggaaga ggtgggtttt accaccgaaa cgctgcacgg tccgctgttg gaagatttat
     4861 ctgcctcggg gatctggctg gttatcagct ccacccacgg tgccggagat attccggaca
     4921 acctttctcc tttctatgaa gcattgcagg aacagaagcc cgatctttct gcggtccgct
     4981 ttggcacaat cggtattggc agtcgtgaat atgacacctt ttgtggggct atcgataaac
     5041 tcgaggcaga actcaaaaat tccggtgcaa aacagacagg cgaaacactg aagatcaaca
     5101 ttcttgatca cgacattccg gaagatccgg cagaagaatg gctgggatcg tggattaatt
     5161 tactcaaata agtatacaga tcgtgcgatc tactgtggat aactctgtca ggaagcttgg
     5221 atcaaccggt agctatccaa agaacaaccg ttgttcagtt tttgagttgt gtataacccc
     5281 tcattctgat cccagcttat acggtccagg atcaccgatc attcacagtt aatgatcctt
     5341 tccaggttgt tgatcttaaa aatcggatcc ttgttatcca cagggcagtg cgatcctaat
     5401 aagagatcac aatagaacag atctctaaat aaatagatct tctttttaat acccaggatc
     5461 ccaggtcttt ctcaagccga caaagttgag tagaatccac ggcccgggct tcaatccatt
     5521 ttcataccgc gttatgcgag gcaatcacca tgttttatcc ggatcctttt gacgtcatca
     5581 tcattggcgg gggtcatgca ggcaccgagg ccgcgatggc tgcggcgcgt atgggtcaac
     5641 agactctgct tttgacacac aatatcgaca ctctggggca gatgagctgc aacccggcga
     5701 tcggcggtat tgggaaggga catctggtaa aagaagtgga tgcactcggc ggtctgatgg
     5761 cgaaagcgat cgatcaagcg ggtatccagt ttaggatact aaacgcaagc aaaggaccgg
     5821 cggttcgcgc tacccgagct caggcggatc gtgtgcgcta ccgtcaggcg gtacgtacgg
     5881 cgctggagaa ccaaccgaac ctgatgatct tccagcaggc ggttgaagat cttattgtcg
     5941 aaaacgatcg cgtggtcggt gctgttaccc aaatgggact gaagttccgt gccaaagctg
     6001 tggtgctcac cgttgggacg ttcctcgacg gcaaaattca tatcggtctg gataattaca
     6061 gcggtggccg tgctggcgat ccgccatcca ttccgctctc tcgccgtttg cgtgaactgc
     6121 cgctgcgcgt tggtcgtctg aaaaccggta ctccgccgcg tattgatgct cgaaccatcg
     6181 actttagcgt gctggcgcaa cagcatggcg ataacccaat gccggtattc tcgtttatgg
     6241 gcaatgcgtc cctgcatccc cagcaggtgc cgtgttatat cactcatacc aacgagaaaa
     6301 cccatgatgt gatccgcagt aacctcgatc gtagcccaat gtatgcaggg gtgatcgaag
     6361 gtgtcggccc acgctactgc ccgtcgatcg aagacaaagt catgcgcttc gcagacagaa
     6421 atcagcatca gatcttcctt gaaccggaag gactgacctc taatgaaatt tatccgaacg
     6481 gtatctccac cagcctgccg ttcgatgtgc agatgcaaat cgtccgctct atgcagggga
     6541 tggaaaacgc gaagatcgtg cgtccgggtt atgccattga gtatgacttc ttcgatcctc
     6601 gcgacctgaa accgacgctg gagagcaagt ttatccaggg gctgttcttt gctggtcaga
     6661 ttaacggcac taccggttac gaagaagccg ctgcgcaagg tttgctggcc ggtcttaacg
     6721 ctgcccgtct gtctgctgac aaagaaggtt gggctccggc acgttctcag gcgtatctcg
     6781 gcgtactggt tgatgacctg tgcactttag gaaccaaaga accgtatcgt atgtttactt
     6841 cgcgcgcaga atatcgtctg atgctgcgcg aagataatgc ggatctgcgt ttgactgaaa
     6901 tcggtcgtga actgggcctg gtggatgacg aacgttgggc gcgctttaac gagaaacttg
     6961 agaatatcga gcgtgagcgt cagcgtctga aatcgacctg ggtaaccccg tcggcggaag
     7021 ctgcagccga agtgaatgct cacctgactg cgccgctttc ccgtgaagcc agtggtgaag
     7081 atctgctgcg tcgtccggaa atgacttatg aaaaattaac cacgctgacg ccgtttgccc
     7141 ctgcgttgac agacgaacag gcggcggaac aggttgagat tcaggttaaa tacgaaggtt
     7201 atatcgcgcg ccagcaagat gagatcgaaa agcagctgcg taacgagaac accctgctac
     7261 ccgcgacact ggattaccgc cagatatccg gtctttctaa cgaagtgatc gccaaactta
     7321 acgatcacaa accggcctct atcggccagg cttcgcgtat ttctggcgtc acgcctgcgg
     7381 ccatctccat tctgctggtg tggctgaaaa aacagggtat gctgcgtcgt agcgcataac
     7441 gcattaaaaa tgcctggtaa gcacccgctt accaggcaac gcatcaagaa caggtaatca
     7501 ccgtgctcaa caaactctcc ttactgctga aagacgcagg tatttcgctt actgatcacc
     7561 agaaaaacca gcttattgcc tacgtgaata tgctgcataa atggaacaaa gcgtacaacc
     7621 tgacttcggt ccgcgatcct aatgagatgc tggtacgcca tattctcgat agcattgtgg
     7681 tggcaccgta tctgcaaggt gaacggttta tcgatgtcgg caccggacca ggactgccag
     7741 gcattccact ctctatcgtg cgtcctgaag cccatttcac tctgttggat agccttggta
     7801 aacgcgtgcg tttccttcgt caggtgcaac atgagcttaa actggagaat attgaaccag
     7861 tacagagcag ggtagaagag tttccttcag agccgccatt tgatggcgta attagccgcg
     7921 cttttgcctc tctgaacgat atggtgagct ggtgccacca tcttcctggt gagcaaggcc
     7981 gtttctacgc gctgaaaggg caaatgccgg aagatgaaat cgctttgttg cccgaagaat
     8041 atcaggtcga atcagtggtt aaacttcagg ttccagccct ggatggcgaa cgtcatctgg
     8101 tggtgattaa agcaaataaa atttaatttt tatcaaaaaa atcataaaaa attgaccggt
     8161 tagactgtta acaacaacca ggttttctac tgatataact ggttacattt aacgccacgt
     8221 tcactctttt gcatcaacaa gataacgtgg ctttttttgg taagcagaaa ataagtcatt
     8281 agtgaaaata tcagtctgct aaaaatcggc gctaagaacc atcattggct gttaaaacat
     8341 tattaaaaat gtcaatgagt ggtttttgtt gtgtaaatgt tatttattaa aacagtatct
     8401 gtttttagac tgaaatatca taaacttgta aaggcatcat ttgccaagta aataaatatg
     8461 ctgtgcgcga acatgcgcaa tatgtgatct gaagcacgct ttatcaccag tgtttacgcg
     8521 ttatttacag tttttcatga tcgaacaggg gtagcggaaa agtcgcaatt gtatgcactg
     8581 gaaaaatatt taaacattta ttcacctttt ggctacttat tgtttgaaat cacgggggcg
     8641 caccgtataa tttgaccgct ttttgatgct tgactctaag ccttaaagaa agttttatac
     8701 gacacgcggc atacctcgaa gggagcagga gtgaaaaacg tgatgtctgt gtcgctcgtg
     8761 agtcgaaacg ttgctcggaa gcttctgctc gttcagttac tggtggtgat agcaagtgga
     8821 ttgctgttca gcctcaaaga ccccttctgg ggcgtctctg caataagcgg gggcctggca
     8881 gtctttctgc ctaacgtttt gtttatgata tttgcctggc gtcaccaggc gcatacacca
     8941 gcgaaaggcc gggtggcttg gacattcgca tttggcgaag ctttcaaagt tctggcgatg
     9001 ttggtgttac tggtggtggc gttggcggtt ttaaaggcgg tattcttgcc gctgatcgtt
     9061 acgtgggttt tggtgctggt ggttcagata ctggcaccgg ctgtaattaa caacaaaggg
     9121 taaaaggcat catggcttca gaaaatatga cgccgcagga ttacatagga caccacctga
     9181 ataaccttca gctggacctg cgtacattct cgctggtgga tccacaaaac cccccagcca
     9241 ccttctggac aatcaatatt gactccatgt tcttctcggt ggtgctgggt ctgttgttcc
     9301 tggttttatt ccgtagcgta gccaaaaagg cgaccagcgg tgtgccaggt aagtttcaga
     9361 ccgcgattga gctggtgatc ggctttgtta atggtagcgt gaaagacatg taccatggca
     9421 aaagcaagct gattgctccg ctggccctga cgatcttcgt ctgggtattc ctgatgaatc
     9481 tgatggattt actgcctatc gacttgctgc cgtacattgc tgaacatgta ctgggtctgc
     9541 ctgcactgcg tgtggttccg tctgcggacg tgaacgtaac gctgtctatg gcactgggcg
     9601 tatttatcct gattctgttc tacaacatca aaatgaaagg catcggcggc ttcacgaaag
     9661 agttgacgct gcagccgttc aatcactggg cgttcattcc tgtcaactta atccttgaag
     9721 gggtaagcct gctgtccaaa ccagtttcac tcggtttgcg actgttcggt aacatgtatg
     9781 ccggtgagct gattttcatt ctgattgctg gtctgttgcc gtggtggtca cagtggatcc
     9841 tgaatgtgcc gtgggccatt ttccacatcc tgatcattac gctgcaagcc ttcatcttca
     9901 tggttctgac gatcgtctat ctgtcgatgg cgtctgaaga acattaattt accaacacta
     9961 ctacgtttta actgaaacaa actggagact gtcatggaaa acctgaatat ggatctgctg
    10021 tacatggctg ccgctgtgat gatgggtctg gcggcaatcg gtgctgcgat cggtatcggc
    10081 atcctcgggg gtaaattcct ggaaggcgca gcgcgtcaac ctgatctgat tcctctgctg
    10141 cgtactcagt tctttatcgt tatgggtctg gtggatgcta tcccgatgat cgctgtaggt
    10201 ctgggtctgt acgtgatgtt cgctgtcgcg tagtaagcgt tgcttttatt taaagagcaa
    10261 tatcagaacg ttaactaaat agaggcattg tgctgtgaat cttaacgcaa caatcctcgg
    10321 ccaggccatc gcgtttgtcc tgttcgttct gttctgcatg aagtacgtat ggccgccatt
    10381 aatggcagcc atcgaaaaac gtcaaaaaga aattgctgac ggccttgctt ccgcagaacg
    10441 agcacataag gaccttgacc ttgcaaaggc cagcgcgacc gaccagctga aaaaagcgaa
    10501 agcggaagcc caggtaatca tcgagcaggc gaacaaacgc cgctcgcaga ttctggacga
    10561 agcgaaagct gaggcagaac aggaacgtac taaaatcgtg gcccaggcgc aggcagaaat
    10621 tgaagccgag cgtaaacgtg cccgtgaaga gctgcgtaag caagttgcta tcctggctgt
    10681 tgctggcgcc gagaagatca tcgaacgttc cgtggatgaa gctgctaaca gcgacatcgt
    10741 ggataaactt gtcgctgaac tgtaaggagg gaggggctga tgtctgaatt tattacggta
    10801 gctcgcccct acgccaaagc agcttttgac tttgccgtcg aacaccaaag tgtagaacgc
    10861 tggcaggaca tgctgacgtt tgccgccgag gtaaccaaaa acgaacaaat ggcagagctt
    10921 ctctctggcg cgcttgcgcc agaaacgctc gccgagtcgt ttatcgcagt ttgtggtgag
    10981 caactggacg aaaacggtca gaacctgatt cgggtaatgg ctgaaaatgg tcgtcttaac
    11041 gcgctcccgg atgttctgga gcagtttatt caccttcgtg ccgtgagtga ggctaccgct
    11101 gaggtagacg tcatttccgc tgccgcactg agtgaacaac agctcgcgaa aatttctgct
    11161 gcgatggaaa aacgtctgtc acgcaaagtt aagctgaatt gcaaaatcga taagtctgta
    11221 atggctggcg ttatcatccg atcgggtgat atggtcattg atggcagcgt acgcggtcgt
    11281 cttgagcgcc ttgcagacgt cttgcagtct taaggggact ggagcatgca actgaattcc
    11341 accgaaatca gcgaactgat caagcagcgc attgctcagt tcaatgttgt gagtgaagct
    11401 cacaacgaag gtactattgt ttctgtaagt gacggtgtta tccgcattca cggcctggcc
    11461 gattgtatgc agggtgaaat gatctccctg ccgggtaacc gttacgctat cgcactgaac
    11521 ctcgagcgcg actctgtagg tgcggttgtt atgggtccgt acgctgacct tgccgaaggc
    11581 atgaaagtta agtgtactgg ccgtatcctg gaagttccgg ttggccgtgg cctgctgggc
    11641 cgtgtggtta acactctggg tgcaccaatc gacggtaaag gtccgctgga tcacgacggc
    11701 ttctctgctg tagaagcaat cgctccgggc gttatcgaac gtcagtccgt agatcagccg
    11761 gtacagaccg gttataaagc cgttgactcc atgatcccaa tcggtcgtgg tcagcgtgaa
    11821 ttgatcatcg gtgaccgtca gacaggtaaa accgcactgg ctatcgatgc catcatcaac
    11881 cagcgcgatt ccggtatcaa atgtatctat gtcgctatcg gccagaaagc gtccaccatt
    11941 tctaacgtgg tacgtaaact ggaagagcac ggcgcactgg ctaacaccat cgttgtggta
    12001 gcaaccgcgt ctgaatccgc tgcactgcaa tacctggcac cgtatgccgg ttgcgcaatg
    12061 ggcgaatact tccgtgaccg cggtgaagat gcgctgatca tttacgatga cctgtctaaa
    12121 caggctgttg cttaccgtca gatctccctg ctgctccgtc gtccgccagg acgtgaagca
    12181 ttcccgggcg acgttttcta cctccactct cgtctgctgg agcgtgctgc acgtgttaac
    12241 gccgaatacg ttgaagcctt caccaaaggt gaagtgaaag ggaaaaccgg ttctctgacc
    12301 gcactgccga ttatcgaaac tcaggcgggt gacgtttctg cgttcgttcc gaccaacgta
    12361 atctccatta ccgatggtca gatcttcctg gaaaccaacc tgttcaacgc cggtattcgt
    12421 cctgcggtta acccgggtat ttccgtatcc cgtgttggtg gtgcagcaca gaccaagatc
    12481 atgaaaaaac tgtccggtgg tatccgtacc gctctggcac agtatcgtga actggcagcg
    12541 ttctctcagt ttgcatccga ccttgacgat gcaacacgta agcagcttga ccacggtcag
    12601 aaagtgaccg aactgctgaa acagaaacag tatgcgccga tgtccgttgc gcagcagtct
    12661 ctggttctgt tcgcagcaga acgtggttac ctggcggatg ttgaactgtc gaaaattggc
    12721 agcttcgaag ccgctctgct ggcttacgtc gaccgtgatc acgctccgtt gatgcaagag
    12781 atcaaccaga ccggtggcta caacgacgaa atcgaaggca agctgaaagg catcctcgat
    12841 tccttcaaag caacccaatc ctggtaacgt ctggaggctt gccttagggc aggccgcaag
    12901 gcattgagga gaagctcatg gccggcgcaa aagagatacg tagtaagatc gcaagcgtcc
    12961 agaacacgca aaagatcact aaagcgatgg agatggtcgc cgcttccaaa atgcgtaaat
    13021 cgcaggatcg catggcggcc agccgtcctt atgcagaaac catgcgcaaa gtgattggtc
    13081 accttgcaca cggtaatctg gaatataagc acccttacct ggaagaccgc gacgttaaac
    13141 gcgtgggcta cctggtggtg tcgaccgacc gtggtttgtg cggtggtttg aacattaacc
    13201 tgttcaaaaa actgctggcg gaaatgaaga cctggaccga caaaggcgtt caatgcgacc
    13261 tcgcaatgat cggctcgaaa ggcgtgtcgt tcttcaactc cgtgggcggc aatgttgttg
    13321 cccaggtcac cggcatgggg gataaccctt ccctgtccga actgatcggt ccggtaaaag
    13381 tgatgttgca ggcctacgac gaaggccgtc tggacaagct ttacattgtc agcaacaaat
    13441 ttattaacac catgtctcag gttccgacca tcagccagct gctgccgtta ccggcatcag
    13501 atgatgatga tctgaaacat aaatcctggg attacctgta cgaacccgat ccgaaggcgt
    13561 tgctggatac cctgctgcgt cgttatgtcg aatctcaggt ttatcagggc gtggttgaaa
    13621 acctggccag cgagcaggcc gcccgtatgg tggcgatgaa agccgcgacc gacaatggcg
    13681 gcagcctgat taaagagctg cagttggtat acaacaaagc tcgtcaggcc agcattactc
    13741 aggaactcac cgagatcgtc tcgggggccg ccgcggttta aacaggttat ttcgtagagg
    13801 atttaagatg gctactggaa agattgtcca ggtaatcggc gccgtagttg acgtcgaatt
    13861 ccctcaggat gccgtaccgc gcgtgtacga tgctcttgag gtgcaaaatg gtaatgagcg
    13921 tctggtgctg gaagttcagc agcagctcgg cggcggtatc gtgcgtacca tcgcaatggg
    13981 ttcctccgac ggtctgcgtc gcggtctgga tgtaaaagac ctcgaacacc cgatcgaagt
    14041 cccggtaggt aaagcgactc tgggccgtat catgaacgta ctgggtgaac cggtcgacat
    14101 gaaaggcgag atcggtgaag aagagcgttg ggcgattcac cgcgcagcac cttcctacga
    14161 agagctgtca aactctcagg aactgctgga aaccggtatc aaagttatcg acctgatgtg
    14221 tccgttcgct aagggcggta aagttggtct gttcggtggt gcgggtgtag gtaaaaccgt
    14281 aaacatgatg gagctcattc gtaacatcgc gatcgagcac tccggttact ctgtgtttgc
    14341 gggcgtaggt gaacgtactc gtgagggtaa cgacttctac cacgaaatga ccgactccaa
    14401 cgttatcgac aaagtatccc tggtgtatgg ccagatgaac gagccgccgg gaaaccgtct
    14461 gcgcgttgct ctgaccggtc tgaccatggc tgagaaattc cgtgacgaag gtcgtgacgt
    14521 tctgctgttc gttgacaaca tctatcgtta caccctggcc ggtacggaag tatccgcact
    14581 gctgggccgt atgccttcag cggtaggtta tcagccgacc ctggcggaag agatgggcgt
    14641 tctgcaggaa cgtatcacct ccaccaaaac tggttctatc acctccgtac aggcggtata
    14701 cgtacctgcg gatgacttga ctgacccgtc tccggcaacc acctttgcgc accttgacgc
    14761 aaccgtggta ctgagccgtc agatcgcgtc tctgggtatc tacccggccg ttgacccgct
    14821 ggactccacc agccgtcagc tggacccgct ggtggttggt caggaacact acgacaccgc
    14881 gcgtggcgtt cagtccatcc tgcaacgtta tcaggaactg aaagacatca tcgccatcct
    14941 gggtatggat gaactgtctg aagaagacaa actggtggta gcgcgtgctc gtaagatcca
    15001 gcgcttcctg tcccagccgt tcttcgtggc agaagtattc accggttctc cgggtaaata
    15061 cgtctccctg aaagacacca tccgtggctt taaaggcatc atggaaggcg aatacgatca
    15121 cctgccggag caggcgttct acatggtcgg ttccatcgaa gaagctgtgg aaaaagccaa
    15181 aaaactttaa cgccttaatc ggagggtgat atggcaatga cttaccacct ggacgtcgtc
    15241 agcgcagagc aacaaatgtt ctctggtctg gtcgagaaaa tccaggtaac gggtagcgaa
    15301 ggtgaactgg ggatctaccc tggccacgca ccgctgctca ccgccattaa gcctggtatg
    15361 attcgcatcg tgaaacagca cggtcacgaa gagtttatct atctgtctgg cggcattctt
    15421 gaagtgcagc ctggcaacgt gaccgttctg gccgacaccg caattcgcgg ccaggatctc
    15481 gacgaagcgc gagccatgga agcgaaacgt aaggctgaag agcacattag cagctctcac
    15541 ggcgacgtag attacgctca ggcgtctgcg gaactggcca aagcgatcgc gcagctgcgc
    15601 gttatcgagt tgaccaaaaa agcgatgtaa caccggcttg aaaagcacaa aagccagtct
    15661 ggaaacaggc tggctttttt tacgcgttga cccgtcctga agagcgttca catagatcct
    15721 gctgatataa aacacccctg ttttcccgtt tattcattga tcgaaataag agcaaaaaca
    15781 tccacctgac gcttaaatga aggtactgcc ttaattttct gcagataaaa ggcgtgacga
    15841 tggtcgaaaa tggcactttc gtcagcgggg ataatccgtt attgaacaat ttatcctctg
    15901 tccatttcac gatgaaaaaa atgtagtttt ttcaaggtga agcggtttaa attcgttctc
    15961 aaattacagt caggacgcgt atgttgaata atgctatgag cgtagtgatc cttgccgcag
    16021 gcaaaggcac gcgcatgtat tccgatcttc cgaaagtgct gcataccctt gccgggaaag
    16081 cgatggttca gcatgtcatt gatgctgcga atgaattagg cgcagcgcac gttcacctgg
    16141 tgtacggtca cggcggcgat ctgcttaaac aggcgctgaa agacgacaac cttaactggg
    16201 tgcttcaggc agagcagctg ggtacgggtc atgcaatgca gcaggccgca cctttctttg
    16261 ccgatgatga agacatttta atgctctacg gcgacgtgcc gctgatctct gtcgaaacac
    16321 tccagcgcct gcgtgatgct aaaccccagg gtggcattgg tctgctgacg gtgaaactgg
    16381 atgatccgac cggttatgga cgtatcaccc gtgaaaatgg caaagttacc ggcattgttg
    16441 agcacaaaga tgccaccgac gagcagcgtc agattcagga gatcaacacc ggcattctga
    16501 ttgccaacgg cgcagatatg aaacgctggc tggcgaagct gaccaacaat aatgctcagg
    16561 gcgaatacta catcaccgac attattgcgc tggcgtatca ggaagggcgt gaaatcgtcg
    16621 ccgttcatcc gcaacgttta agcgaagtag aaggtgtgaa taaccgcctg caactctccc
    16681 gtctggagcg cgtttaccag tccgaacagg ctgaaaaact gctgttagca ggcgttatgc
    16741 tgcgcgatcc ggcgcgtttt gatctgcgcg gtacgcttac tcacgggcgc gatgttgaaa
    16801 ttgatactaa cgttatcatc gagggcaacg tgactctcgg tcatcgcgtg aaaatcggca
    16861 ccggttgcgt gattaaaaac agcgtgattg gcgatgattg cgaaatcagt ccgtataccg
    16921 ttgtggaaga cgcgaatctg gcagcggcct gtactattgg cccgtttgcc cgtctgcgtc
    16981 ctggtgctga gttgctggaa ggtgcacacg ttggtaactt cgttgagatg aaaaaagcgc
    17041 gtctgggtaa aggcacgaaa gccgggcatc tgacttacct gggcgatgcg gaaattggcg
    17101 ataacgttaa catcggcgcg ggaaccatta cctgcaacta cgatggtgcg aataaattta
    17161 agaccattat cggcgacgat gtgtttgtcg gttccgacac tcagctggtg gccccggtaa
    17221 cagtaggcaa aggcgcgacc attgctgcgg gtacaactgt gacgcgtaat gtcggcgaaa
    17281 atgcattagc tatcagccgt gtgccgcaga ctcagaaaga aggctggcgt cgtccggtaa
    17341 agaaaaagta attctggccg ggtaatccgg tcatctggga tgaggagata acataatctc
    17401 cctcccacaa gcagtaacta taaaaataac cccactctct acaaggctcg gggcgcccga
    17461 aaaaacgggc atacaggttg accgacaacg atataaatcg gaatcaaaaa ctatgtgtgg
    17521 aattgttggc gcgatcgcgc aacgtgatgt agcagaaatc cttcttgaag gtttacgtcg
    17581 tctggaatac cgcggatatg actctgccgg tctggccgtt gttgatgcgg aaggtcatat
    17641 gactcgcctg cgtcgcctcg gtaaagtcca gatgctggct caggcagcgg aagaacatcc
    17701 tctgcatggc ggcaccggta ttgctcacac tcgctgggcg acccacggtg aaccttcaga
    17761 agctaacgcg catccgcatg tttctgaaca cattgtggtg gtgcataatg gcatcatcga
    17821 aaaccatgaa ccgctgcgtg aagagttaaa agcgcgtggc tataccttcg tttctgaaac
    17881 cgacaccgaa gtgattgccc atctggtgaa ctgggagctg aaacaaggcg ggactctgcg
    17941 tgaggctgtt ctgcgtgcta tcccgcagct gcgcggtgcg tacggtacag tgatcatgga
    18001 ctcccgtcac ccggataccc tgctagcggc acgttctggt agtccgctgg tgattggcct
    18061 gggtatgggc gaaaacttta tcgcttctga ccagctggcg ctgttgccgg tgacccgtcg
    18121 ctttatcttc cttgaagagg gcgatattgc ggaaatcact cgccgttcgg taaacatctt
    18181 cgataaaact ggcgcggaag taaaacgtca ggatatcgaa tccaatctgc aatatgacgc
    18241 gggcgataaa ggtatttacc gtcactacat gcagaaagag atctacgaac agccgaacgc
    18301 gataaaaaac acccttaccg ggcgcatcag ccacggtcag gttgatttaa gcgagctggg
    18361 accgaacgcc gacgaactgc tgtcgaaggt tgagcatatt cagatcctcg cctgtggtac
    18421 ttcttataac tccggtatgg tttcccgcta ctggtttgaa tcgctggcag gtattccgtg
    18481 cgacgtcgaa atcgcctctg aattccgcta tcgtaaatct gccgtgcgtc gtaacagcct
    18541 gatgatcacc ttgtcacagt ctggcgaaac cgcggatacc ctggcgggcc tgcgtctgtc
    18601 gaaagagctg ggttaccttg gttcgctggc aatctgtaac gttccgggtt cttctctggt
    18661 gcgcgaatcc gatctggcgc tgatgaccaa cgcgggtacg gaaatcggcg tggcgtccac
    18721 taaagcattc accactcagt taactgtgct gttgatgctg gtggcgaagc tgtctcgcct
    18781 gaaaggtctg gatgcctcca ttgaacatga cattgtgcat ggtctgcagg cgctgccgag
    18841 ccgtattgag cagatgctgt ctcaggataa acgcattgaa gctctggcag aagatttctc
    18901 tgacaaacat cacgcgctgt tcctgggccg tggcgatcag tacccaatcg cgctggaagg
    18961 cgcattgaag ctgaaagaga tctcttacat tcacgctgaa gcctacgctg caggcgagct
    19021 gaaacacggc ccgctggcgc tgattgacgc ggatatgccg gttatcgtcg ttgcaccgaa
    19081 caacgaactg ttggaaaaac tgaaatccaa cattgaagaa gttcgcgcgc gtggcggtca
    19141 attgtatgtc ttcgccgatc aggatgcggg ttttgtaagt agcgataaca tgcacatcat
    19201 cgagatgccg catgtggaag aggtgattgc accgatcttc tacaccgttc cgctgcagct
    19261 gctggcttac catgtcgcgc tgatcaaagg caccgacgtt gaccagccgc gtaacctggc
    19321 aaaatcggtt acggttgagt aatcactatc atagataaaa aagggctgct gtgggcggac
    19381 aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc ttaaactaga
    19441 cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata ctggactttt
    19501 gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag
    19561 gggcgtggcc aagggcatgg taaagactat attccatggc taacagtaca agaagttcct
    19621 tcttcaggtc gttcccaccg tatttattct cataagacgg gacgagtcca tcatttgcta
    19681 tctgacttag agcttgctgt ttttctcagt cttgagtggg agagcagcgt gctagatata
    19741 cgcgagcagt tccccttatt acctagtgat accaggcaga ttgcaataga tagtggtatt
    19801 aagcatcctg ttattcgtgg tgtagatcag gttatgtcta ctgatttttt agtggactgc
    19861 aaagatggtc cttttgagca gtttgctatt caagtcaaac ctgcagcagc cttacaagac
    19921 gagcgtacct tagaaaaact agaactagag cgtcgctatt ggcagcaaaa gcaaattcct
    19981 tggttcattt ttactgataa agaaataaat cccgtagtaa aagaaaatat tgaatggctt
    20041 tattcagtga aaacagaaga agtttctgcg gagcttttag cacaactatc cccattggcc
    20101 catatcctgc aagaaaaagg agatgaaaac attatcaatg tctgtaagca ggttgatatt
    20161 gcttatgatt tggagttagg caaaacattg agtgagatac gagccttaac cgcaaatggt
    20221 tttattaagt tcaatattta taagtctttc agggcaaata agtgtgcaga tctctgtatt
    20281 agccaagtag tgaatatgga ggagttgcgc tatgtggcaa attaatgagg ttgtgctatt
    20341 tgataatgat ccgtatcgca ttttggctat agaggatggc caagttgtct ggatgcaaat
    20401 aagcgctgat aaaggagttc cacaagctag ggctgagttg ttgctaatgc agtatttaga
    20461 tgaaggccgc ttagttagaa ctgatgaccc ttatgtacat cttgatttag aagagccgtc
    20521 tgtagattct gtcagcttcc agaagcgcga ggaggattat cgaaaaattc ttcctattat
    20581 taatagtaag gatcgtttcg accctaaagt cagaagcgaa ctcgttgagc atgtggtcca
    20641 agaacataag gttactaagg ctacagttta taagttgtta cgccgttact ggcagcgtgg
    20701 tcaaacgcct aatgcattaa ttcctgacta caaaaacagc ggtgcaccag gggaaagacg
    20761 ttcagcgaca ggaacagcaa agattggccg agccagagaa tatggtaagg gtgaaggaac
    20821 caaggtaacg cccgagattg aacgcctttt taggttgacc atagaaaagc acctgttaaa
    20881 tcaaaaaggt acaaagacca ccgttgccta tagacgattt gtggacttgt ttgctcagta
    20941 ttttcctcgc attccccaag aggattaccc aacactacgt cagtttcgtt atttttatga
    21001 tcgagaatac cctaaagctc agcgcttaaa gtctagagtt aaagcagggg tatataaaaa
    21061 agacgtacga cccttaagta gtacagccac ttctcaggcg ttaggccctg ggagtcgtta
    21121 tgagattgat gccacgattg ctgatattta tttagtggat catcatgatc gccaaaaaat
    21181 cataggaaga ccaacgcttt acattgtgat tgatgtgttt agtcggatga tcacgggctt
    21241 ttatatcggc tttgaaaatc cgtcttatgt ggtggcgatg caggcttttg taaatgcttg
    21301 ctctgacaaa acggccattt gtgcccagca tgatattgag attagtagct cagactggcc
    21361 gtgtgtaggt ttgccagatg tgttgctagc ggaccgtggc gaattaatga gtcatcaggt
    21421 cgaagcctta gtttctagtt ttaatgtgcg agtggaaagt gctccaccta gacgtggcga
    21481 tgctaaaggc atagtggaaa gcacttttag aacactacaa gccgagttta agtcctttgc
    21541 acctggcatt gtagagggca gtcggatcaa aagccatggt gaaacagact ataggttaga
    21601 tgcatctctg tcggtatttg agttcacaca aattattttg cgtacgatct tattcagaaa
    21661 taaccatctg gtgatggata aatacgatcg agatgctgat tttcctacag atttaccgtc
    21721 tattcctgtc cagctatggc aatggggtat gcagcatcgt acaggtagtt taagggctgt
    21781 ggagcaagag cagttgcgag tagcgttact gcctcgccga aaggtctcta tttcttcatt
    21841 tggcgttaat ttgtggggtt tgtattactc ggggtcagag attctgcgtg agggttggtt
    21901 gcagcggagc actgatatag ctagacctca acatttagaa gcggcttatg acccagtgct
    21961 ggttgatacg atttatttgt ttccgcaagt tggcagccgt gtattttggc gctgtaatct
    22021 gacggaacgt agtcggcagt ttaaaggtct ctcattttgg gaggtttggg atatacaagc
    22081 acaagaaaaa cacaataaag ccaatgcgaa gcaggatgag ttaactaaac gcagggagct
    22141 tgaggcgttt attcagcaaa ccattcagaa agcgaataag ttaacgccca gtactactga
    22201 gcccaaatca acacgcatta agcagattaa aactaataaa aaagaagccg tgacctcgga
    22261 gcgtaaaaaa cgtgcggagc atttgaagcc aagctcttca ggtgatgagg ctaaagttat
    22321 tcctttcaac gcagtggaag cggatgatca agaagattac agcctaccca catacgtgcc
    22381 tgaattattt caggatccac cagaaaagga tgagtcatga gtgctacccg gattcaagca
    22441 gtttatcgtg atacgggggt agaggcttat cgtgataatc cttttatcga ggccttacca
    22501 ccattacaag agtcagtgaa tagtgctgca tcactgaaat cctctttaca gcttacttcc
    22561 tctgacttgc aaaagtcccg tgttatcaga gctcatacca tttgtcgtat tccagatgac
    22621 tattttcagc cattaggtac gcatttgcta ctaagtgagc gtatttcggt catgattcga
    22681 ggtggctacg taggcagaaa tcctaaaaca ggagatttac aaaagcattt acaaaatggt
    22741 tatgagcgtg ttcaaacggg agagttggag acatttcgct ttgaggaggc acgatctacg
    22801 gcacaaagct tattgttaat tggttgttct ggtagtggga agacgacctc tcttcatcgt
    22861 attctagcca cgtatcctca ggtgatttac catcgtgaac tcaatgtaga gcaggtggtg
    22921 tatttgaaaa tagactgctc gcataatggt tcgctaaaag aaatctgctt gaattttttc
    22981 agagcgttgg atcgagcctt gggctcgaac tatgagcgtc gttatggctt aaaacgtcat
    23041 ggtatagaaa ccatgttggc tttgatgtcg caaatagcca atgcacatgc tttagggttg
    23101 ttggttattg atgaaattca gcatttaagc cgctctcgtt cgggtggatc tcaagagatg
    23161 ctgaactttt ttgtgacgat ggtgaatatt attggcgtac cagtgatgtt gattggtacc
    23221 cctaaagcac gagagatttt tgaggctgat ttgcggtctg cacgtagagg ggcagggttt
    23281 ggagctatat tctgggatcc tatacaacaa acgcaacgtg gaaagcccaa tcaagagtgg
    23341 atcgctttta cggataatct ttggcaatta cagcttttac aacgcaaaga tgcgctgtta
    23401 tcggatgagg tccgtgatgt gtggtatgag ctaagccaag gagtgatgga cattgtagta
    23461 aaactttttg tactcgctca gctccgtgcg ctagctttag gcaatgagcg tattaccgct
    23521 ggtttattgc ggcaagtgta tcaagatgag ttaaagcctg tgcaccccat gctagaggca
    23581 ttacgctcgg gtatcccaga acgcattgct cgttattctg atctagtcgt tcccgagatt
    23641 gataaacggt taatccaact tcagctagat atcgcagcga tacaagaaca aacaccagaa
    23701 gaaaaagccc ttcaagagtt agataccgaa gatcagcgtc atttatatct gatgctgaaa
    23761 gaggattacg attcaagcct gttaattccc actattaaaa aagcgtttag ccagaatcca
    23821 acgatgacaa gacaaaagtt actgcctctt gttttgcagt ggttgatgga aggcgaaacg
    23881 gtagtgtcag aactagaaaa gccctccaag agtaaaaagg tttcggctat aaaggtagtc
    23941 aagcccagcg actgggatag cttgcctgat acggatttac gttatatcta ttcacaacgc
    24001 caacctgaaa aaaccatgca tgaacggtta aaagggaaag gggtaatagt ggatatggcg
    24061 agcttattta aacaagcagg ttagccatga gaaactttcc tgttccgtac tcgaatgagc
    24121 tgatttatag cactattgca cgggcaggcg tttatcaagg gattgttagt cctaagcagc
    24181 tgttggatga ggtgtatggc aaccgcaagg tggtcgctac cttaggtctg ccctcgcatt
    24241 taggtgtgat agcaagacat ctacatcaaa caggacgtta cgctgttcag cagcttattt
    24301 atgagcatac cttattccct ttatatgctc cgtttgtagg caaggagcgc cgagacgaag
    24361 ctattcggtt aatggagtac caagcgcaag gtgcggtgca tttaatgcta ggagtcgctg
    24421 cttctagagt taagagcgat aaccgcttta gatactgccc tgattgcgtt gctcttcagc
    24481 taaataggta tggggaagcc ttttggcaac gagattggta tttgcccgct ttgccatatt
    24541 gtccaaaaca cggtgcttta gtcttctttg atagagctgt agatgatcac cgacatcaat
    24601 tttgggcttt gggtcatact gagctgcttt cagactaccc caaagactcc ctatctcaat
    24661 taacagcact agctgcttat atagcccctc tgttagatgc tccacgagcg caagagcttt
    24721 ccccaagcct tgagcagtgg acgctgtttt atcagcgctt agcgcaggat ctagggctaa
    24781 ccaaaagcaa gcacattcgt catgacttgg tggcggagag agtgaggcag acttttagtg
    24841 atgaggcact agagaaactg gatttaaagt tggcagagaa caaggacacg tgttggctga
    24901 aaagtatatt ccgtaagcat agaaaagcct ttagttattt acagcatagt attgtgtggc
    24961 aagccttatt gccaaaacta acggttatag aagcgctaca gcaggcaagt gctcttactg
    25021 agcactctat aacgacaaga cctgttagcc agtctgtgca acctaactct gaagatttat
    25081 ctgttaagca taaagactgg cagcaactag tgcataaata ccaaggaatt aaggcggcaa
    25141 gacagtcttt agagggtggg gtgctatacg cttggcttta ccgacatgac agggattggc
    25201 tagttcactg gaatcaacag catcaacaag agcgtctggc acccgcccct agagttgatt
    25261 ggaaccaaag agatcgaatt gctgtacgac aactattaag aatcataaag cgtctagata
    25321 gtagccttga tcacccaaga gcgacatcga gctggctgtt aaagcaaact cctaacggaa
    25381 cctctcttgc aaaaaatcta cagaaactgc ctttggtagc gctttgctta aagcgttact
    25441 cagagagtgt ggaagattat caaattagac ggattagcca agcttttatt aagcttaaac
    25501 aggaagatgt tgagcttagg cgctggcgat tattaagaag tgcaacgtta tctaaagagc
    25561 ggataactga ggaagcacaa agattcttgg aaatggttta tggggaagag tgagtggtta
    25621 ggctagctac atttaatgac aatgtgcagg ttgtacatat tggtcattta ttccgtaact
    25681 cgggtcataa ggagtggcgt atttttgttt ggtttaatcc aatgcaagaa cggaaatgga
    25741 ctcgatttac tcatttgcct ttattaagtc gagctaaggt ggttaacagt acaacaaagc
    25801 aaataaataa ggcggatcgt gtgattgagt ttgaagcatc ggatcttcaa cgagccaaaa
    25861 taatcgattt tcctaatctc tcgtcctttg cttccgtacg caacaaggat ggagcgcaga
    25921 gttcatttat ttacgaagct gaaacaccat atagcaagac tcgttatcac atcccacagt
    25981 tagagctagc tcggtcatta tttttaatta actcctattt ctgtcgaagc tgtttgagca
    26041 gtaccgcttt acagcaagag ttcgacgttc agtatgaggt tgagcgagat catttagaga
    26101 taaggatctt acccagttca tcgtttccta aaggggcgtt agagctgtcg gccgtagtgc
    26161 agcttttggt ttggttgttt tcggatcaag atgttatgga ttcgtatgaa agtattttta
    26221 ggcactatca acaaaataga gaaattaaga acggcgttga aagctggtgc tttagctttg
    26281 accctccgcc catgcagggt tggaaattac atgtaaaagg acgttcttct aacgaggata
    26341 aggattattt agttgaggaa atagtaggtt tagaaatcaa cgctatgctt cctagcacaa
    26401 cagctattag ccatgcctct tttcaggaaa aggaggcagg tgatggtagt acgcagcaca
    26461 tagcggtttc aacagagtca gttgttgatg atgagcatct acagttggac gatgaggaaa
    26521 cagccaatat agacacagac acacgagtca tagaggctga gccgacatgg ataagtttta
    26581 gtagacctag tcgaattgaa aaatctcgca gggcaagaaa aagtagccaa actattttag
    26641 aaaaagaaga agcaacaaca agtgaaaata gtaatttggt tagtactgat gagccacact
    26701 taggtggtgt cctagcagcg gcagatgtgg gtgggaagca ggatgcaacc aattacaact
    26761 ctatttttgc taatcgattt gctgcttttg atgagctact ttcaattcta aaaactaaat
    26821 ttgcatgtcg ggtgcttttt gaagaaacct tggttttgcc aaaagttggg cgtagccgat
    26881 tacatctgtg taaagatggc tcaccaagag tgattaaagc cgttggggtg caacgtaatg
    26941 gcagtgaatt tgtattgcta gaggtggatg catcggatgg ggtgaaaatg ctttctacca
    27001 aagtgttgag tggcgttgat agcgaaacat ggcggaatga ttttgaaaag atacggcgtg
    27061 gagtggtgaa gagctcattg aattggccaa atagtttgtt tgatcaatta tatggacaag
    27121 acgggcatag aggggtgaat catccaaagg ggttggggga gctgcaagta tcgagagagg
    27181 atatggaagg gtgggctgag agagtggtta gagagcaatt tacgcattaa aggaatgact
    27241 gaaagagcct gtaaaccctt ttgtgtaagt gcttttgccg gtcagttaaa ggtggccatt
    27301 taaacggtca ccaaattcga tcataaaacg gttcatggcc ggcttccagt tgcggatcgg
    27361 catcgtccat ttcttggtcg ccgcctggat agccaggtac accaccttca tcgctgattc
    27421 gtccgtaggg aacaccttga gttcatatta atggaatttt ctacaaatag cctccgtggt
    27481 tttgaggggg gattacagac gatccatagt agtaatccaa tgagttcttg agcgcggcga
    27541 catgtttgga cgccttggca aaaattagag cctgcttgaa gtgcaggcga gcacgtgctt
    27601 ggacgattga tccattcgcg gtcaaaaact caatcttgga tgacagcgtg tcaggaaatc
    27661 caatatcgaa gtcccgcccc gtgtcaaagt agggcatttc atgatcaaag gacgaagctt
    27721 cccaggccac ttccctaata ttccagccgc tagaaacaag atttcgtacg acttcatcaa
    27781 cttttttgct ttccaacttg tcacaaagtg cataggcaag agctttggcg gcagacacgt
    27841 gcgtttcctc ttaaacatag gtcgaacgga tacccgaggc aaagcggagc attagtccca
    27901 aagcatctcc ggtagatccg gaggctccat actgaaggca tggcatcttg ggcattgata
    27961 tttgaaactt ctctctagat agcagggatt tcttggcctt gcctcatgct gatagcttgt
    28021 aaatatcaca cgatccttag ccccacaaag tgagcatact ccgtagcttt catttataga
    28081 aatcgtcgtt agattgtcgc agcatgtgca cgttccgact atttgctcag gaagcatcgc
    28141 cataccttgg cctaggtaaa gaagatcaga ctcgtagcca catcggtggc aactgagctt
    28201 gtacgcaatg cccatctggt tgtctcacct gaatcgtaac gtcagagcta ttcatccatc
    28261 cattcggcat caatgtatgc cactaagccg accagctgac cgtttggact gaagtatgtt
    28321 gcctgcaccc cacttgacat ctcatcaata ccaccgcact gcgctaggcg ccggtcccac
    28381 ttgcccacag aagcgtcagg atcggctgag ttccccaaga cccttgtgag ccatgcctcc
    28441 tttaactcaa cctctcgaat gattgcgtcg gccagctcct ccgcgttatc tagttcgaca
    28501 tagtctggaa ttcggacgta cgttctgaac acagctggat acttacttgg gcgattgtca
    28561 tacatgacat caacaatgta cccgtttgtg taaccgtctc ttggaggttc gtatgacact
    28621 agtggttccc ctcagcttgc gactagatgt tgaggcctaa cattttatta gagagcaggc
    28681 tagttgctta gatacatgat cttcaggccg ttatctgtca gggcaagcga aaattggcca
    28741 tttatgacga ccaatgcccc gcagaagctc ccatctttgc cgccatagac gccgcgcccc
    28801 ccttttgggg tgtagaacat ccttttgcca gatgtggaaa agaagttcgt tgtcccattg
    28861 ttggcaatga cgtagtagcc ggcgaaagtg cgagacccat ttgcgctata tataagccta
    28921 cgatttccgt tgcgactatt gtcgtaattg gatgaactat tatcgtagtt gctctcagag
    28981 ttgtcgtaat ttgatggact attgtcgtaa ttgcttatgg agttgtcgta gttgcttgga
    29041 gaaatgtcgt agttggatgg ggagtagtca tagggaagac gagcttcatc cactaaaaca
    29101 attggcaggt cagcaagtgc ctgccccgat gccatcgcaa gtacgaggct tagaaccacc
    29161 ttcaacagat cgcgcatagt cttccccagc tctctaacgc ttgagttaag ccgcgccgcg
    29221 aagcggcgtc ggcttgaacg aattgttaga cattatttgc cgactacctt ggtgatctcg
    29281 cctttcacgt agtgaacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct
    29341 tgtccaagat aagcctgcct agcttcaagt atgacgggct gatactgggc cggcaggcgc
    29401 tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac
    29461 caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag
    29521 ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca
    29581 aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc
    29641 aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg
    29701 cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg
    29761 tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc
    29821 gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc
    29881 accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg
    29941 tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct
    30001 gatagttgag tcgatacttc ggcgatcacc gcttccctca tgatgtttaa ctcctgaatt
    30061 aagccgcgcc gcgaagcggt gtcggcttga atgaattgtt aggcgtcatc ctgtgctccc
    30121 gagaaccagt accagtacat cgctgtttcg ttcgagactt gaggtctagt tttatacgtg
    30181 aacaggtcaa tgccgccgag agtaaagcca cattttgcgt acaaattgca ggcaggtaca
    30241 ttgttcgttt gtgtctctaa tcgtatgcca aggagctgtc tgcttagtgc ccactttttc
    30301 gcaaattcga tgagactgtg cgcgactcct ttgcctcggt gcgtgtgcga cacaacaatg
    30361 tgttcgatag aggctagatc gttccatgtt gagttgagtt caatcttccc gacaagctct
    30421 tggtcgatga atgcgccata gcaagcagag tcttcatcag agtcatcatc cgagatgtaa
    30481 tccttccggt aggggctcac acttctggta gatagttcaa agccttggtc ggataggtgc
    30541 acatcgaaca cttcacgaac aatgaaatgg ttctcagcat ccaatgtttc cgccacctgc
    30601 tcagggatca ccgaaatctt catatgacgc ctaacgcctg gcacagcgga tcgcaaacct
    30661 ggcgcggctt ttggtacaaa aggcgtgaca ggtttgcgaa tccgttgctg ccacttgtta
    30721 acccttttgc cagatttggt aactataatt tatgttagag gcgaagtctt gggtaaaaac
    30781 tggcctaaaa ttgctgggga tttcaggaaa gtaaacatca ccttccggct cgatgtctat
    30841 tgtagatata tgtagtgtat ctacttgatc gatcaggctt ttgtatatct ccccaccacc
    30901 tgaaacaatg acatgatccg ttattttctt taggttggtt aaagcatctt taattgatgg
    30961 aaagatcaat acgttctcat tgtcagatgt aaaacttgaa cgtgttacga ccgcatactt
    31021 tcggttgggt aatgctccca ttgattcaaa agtcttgcgt ccaaccaaca gccattggtt
    31081 ataggtaata gctttaaaca ggagctgttc acctttggca ctccatggaa tatcagggcc
    31141 attcccgata actccattct tcgatatagc taccattagt gatagtttca caattcttcc
    31201 tcagaggtta acattttatt agtaagcatg ctcgttttgt cacccgctga tgcttaccgt
    31261 taattaatat aatcacttgt attaaataga ctttctttaa aaatacaaga cactctgtta
    31321 ttacaaatcg tgcatgccgt ctatcctgct tgcacgatgc acattatctc acccaaagaa
    31381 ctcataacat tttaatttat ttagcattaa attaaaactc taccggtaaa caagcatctc
    31441 taggcgtaaa aaacaggcac ttttttaaat cccagctaga ataggctgta taggcagaca
    31501 gttgcaagac aaggagcgtt ttatgtctaa cagtccattt ttaaattcta tacgcacgga
    31561 tatgcgacaa aaaggttatg cgctgaaaac tgaaaaaact tacctgcact ggattaagcg
    31621 ttttattctg tttcacaaaa aacgtcatcc tcagaccatg ggcagtgaag aggtcaggct
    31681 gtttttatcc agcttagcaa acagcagaca tgtagccata aacacgcaga aaatcgcttt
    31741 aaatgcccta gcttttttgt acaacaggtt tttacaacag ccgttgggcg atattgatta
    31801 tatccctgca agcaagccta gacggctacc ctctgttatc tctgcaaatg aagtgcaacg
    31861 cattttgcag gttatggata ctcgcaacca agttattttt acgctgctgt atggtgcagg
    31921 tttgcgcatt aatgaatgct tgcgtttgcg ggttaaagat tttgattttg ataatggctg
    31981 catcactgtg catgacggta agggtgggaa aagcagaaac agcctactgc ccacgcgcct
    32041 aatcccagca ataaaataac tcattgagca agcgcggctt attcagcaag acgacaactt
    32101 acaaggcgta gggccatcgc tgccttttgc tttagatcac aaataccctt ctgcttatcg
    32161 acaagcggcg tggatgtttg tctttccctc cagcacgctc tgcaaccacc cgtataacgg
    32221 caaattatgc cgccatcatc tgcatgactc cgttgcgcga aaggcattga aggcagccgt
    32281 acaaaaagca ggcatcgtta gcaagcgtgt cacttgtcat acatttcgtc actcgtttgc
    32341 tacgcatcta ttacaagcgg ggcgtgatat tcgcactgtg caagaactct tagggcataa
    32401 cgatgttaag accacgcaaa tctatacgca tgtgttgggt cagcattttg ccggcaccac
    32461 cagtcctgcg gatggactga tgctacttat caatcagtaa aagtagataa atatctttat
    32521 gtagccctga gtagcccctg atacttttta tggtgaaaaa gataaacaat catagagggg
    32581 ataaatatga gccagttcta ccattgttca gataagttta agcaagatac tgtcaatcac
    32641 agtgaccatt catggtcatt acacttcctc cagttgccag acaatgccta acataaaaac
    32701 gcaaaagccc aaaagtcaga gcttaggtgt tctaggcgtt ttctaagcgc tgaagaaata
    32761 cgtcttaccg tagattgcgt gcgtttctga agcacatttt ccttatacat ggctttatcc
    32821 caggcagctt catcaagctc ttggagtaac aagccagtaa taatgcgaca ctctcgggtt
    32881 tgtagcgagc cgctggtgag atcggagtta tagcggaaag gggtcatttg taattatttg
    32941 tgggtggagg atagttaact tattattgta gttggaagag gctgctttcc aaacattagc
    33001 acctcgtaat gcttgttact gtatattaat ttatgctctt agcgcatgtt aatgggctat
    33061 tacaatgcat aatgtccaca tcaagataac tgtggcttaa cacctgtaca agcgaaaaaa
    33121 atcaatcacc tgtccggaaa tacgggatca ctgtaaagtg ttggtgaggc agtctaggag
    33181 ggggatttaa tttgggatac tgatgaaagg attatgatca gttggatata ggatcgtact
    33241 aagctgagcg gcaggttagc tagattagtt aaccagataa gtgaaatcta gttccaaact
    33301 attttgtcat ttttaatttt cgtattagct tacgacgcta cacccagttc ccatctattt
    33361 tgtcactctt ccctaaataa tccttaaaaa ctccatttcc acccctccca gttcccaact
    33421 attttgtccg cccacagctg ccgaccgg
//
LOCUS       DAJTUI010000039        32310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTUI010000039 DAJTUI010000000
VERSION     DAJTUI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.039"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(2..94)
                     /locus_tag="OO464_001972"
     tRNA            complement(2..94)
                     /locus_tag="OO464_001972"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO464_001973"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO464_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6227131.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO464_001974"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO464_001974"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6227132.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO464_001975"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO464_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6227133.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO464_001976"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO464_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6227134.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO464_001977"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO464_001977"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6227135.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO464_001978"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO464_001978"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6227136.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OO464_001979"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OO464_001979"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OO464_001980"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OO464_001980"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6227137.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OO464_001981"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OO464_001981"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6227138.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OO464_001982"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OO464_001982"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR6227139.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OO464_001983"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OO464_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR6227140.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OO464_001984"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OO464_001984"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6227141.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OO464_001985"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OO464_001985"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6227142.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO464_001986"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO464_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6227143.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OO464_001987"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OO464_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR6227144.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OO464_001988"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OO464_001988"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR6227145.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO464_001989"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO464_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6227146.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO464_001990"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO464_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6227147.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO464_001991"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO464_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6227148.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OO464_001992"
     CDS             complement(20900..21019)
                     /locus_tag="OO464_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR6227149.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO464_001993"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO464_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR6227150.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO464_001994"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO464_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6227151.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25499)
                     /gene="hycC"
                     /locus_tag="OO464_001995"
     CDS             complement(23682..25499)
                     /gene="hycC"
                     /locus_tag="OO464_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6227152.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKS
                     VLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIY
                     QSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATC
                     APLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACP
                     LLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKW
                     LNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25536..26147)
                     /gene="hycB"
                     /locus_tag="OO464_001996"
     CDS             complement(25536..26147)
                     /gene="hycB"
                     /locus_tag="OO464_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6227153.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26272..26733)
                     /gene="hycA"
                     /locus_tag="OO464_001997"
     CDS             complement(26272..26733)
                     /gene="hycA"
                     /locus_tag="OO464_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6227154.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26945..27295
                     /gene="hypA"
                     /locus_tag="OO464_001998"
     CDS             26945..27295
                     /gene="hypA"
                     /locus_tag="OO464_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6227155.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27299..28171
                     /gene="hypB"
                     /locus_tag="OO464_001999"
     CDS             27299..28171
                     /gene="hypB"
                     /locus_tag="OO464_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6227156.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28162..28434
                     /gene="hypC"
                     /locus_tag="OO464_002000"
     CDS             28162..28434
                     /gene="hypC"
                     /locus_tag="OO464_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6227157.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28434..29555
                     /gene="hypD"
                     /locus_tag="OO464_002001"
     CDS             28434..29555
                     /gene="hypD"
                     /locus_tag="OO464_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6227158.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29552..30562
                     /gene="hypE"
                     /locus_tag="OO464_002002"
     CDS             29552..30562
                     /gene="hypE"
                     /locus_tag="OO464_002002"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6227159.1"
                     /translation="MNNIQLAHGSGSQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30636..>32310
                     /gene="flhA"
                     /locus_tag="OO464_002003"
     CDS             30636..>32310
                     /gene="flhA"
                     /locus_tag="OO464_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR6227160.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPED"
BASE COUNT         7620 a         9014 c         8412 g         7264 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag ccccagcccc agccccagcc ccagcaggat aatgccgata
    24661 ttttccaggg tgtggtaagc cagcaggcgc tggatgttgt gttccatcaa cgcatacaga
    24721 ccaccgacga acgcggtgat catgccgagc accagcagcg cgatccccca ccacagcggt
    24781 gcattaccgc ccagcagtga cagggttaac atgcccagca ggccaatttt catgactacc
    24841 gtagaaaaca acgcggcagc tggcgcagag gcgttagcat gtgcctgcgg cacccagccg
    24901 tgcagcggaa taatcccagc cagcaggcca aagccaatca caccgagcag ccagatatcg
    24961 gaaccgagcg gcagctgttg catacgcata tccagcaggc gcagatccag cgtgccataa
    25021 cgctgccaca gcagccagca agcaatcacc agcagcagag tgccaagacg cctcagcgca
    25081 aaccacagtt tgccctcttt gctgttgctg gtgaggaaca ccgcacacaa ggccatgact
    25141 tcggccatta ccacgaacat gccgaggttg ctggcgatga cggcgcagac ggcggcagcc
    25201 atcaacatat tgatctgcaa gccgttgcac ttcacctgcg cgtggcgatg ccagtcaatg
    25261 ttatagaggc tgacaaacag accgcaaagg ccgagggtaa tcagccaaat cgcgttaagc
    25321 ggggagattt gcacatcgta gcttaccagc gacagcgcac cgctcacgcc aaccgcgcca
    25381 gtcagtacag tgaagcccgc ggctgccgta tacagactac caaccgcgcc gccaattccg
    25441 gctatccagc cacttagcgc tttttgaaaa gagaaatgcc agaacagcgg cggcgacaaa
    25501 ccatgccacg ccgctattga tcagggaaat tgcgctcatt tagcctctcc actttgagcc
    25561 tgctgaaaca aggtgagatc gccaaagtcc gtgttaaagg tcagctcacg cttacgtttg
    25621 ctgacgcggg cgatatcggt gttatccacc agatgcaggg ctttagtcgg gcacatccgc
    25681 acacaggccg gaccttgttc atcaaagcta caaaggtcac atttgacggc gatcgcgcga
    25741 atacctggca cccagtcaag caatgtgctg acacgcgccg gagcaggcgg tgccggtggc
    25801 gctttcgggg tattggcgtt tgccggaata tccagcggac ggctgccgga aaattcaatt
    25861 gcgccaaacg ggcaggcgat gccgcacagt ttgcagctta cgcacagact ttcattcaac
    25921 tgcacggccc catcaacgcg ggtgatggcg ttaaccgggc agaccaccgc gcagggtgca
    25981 tcttcacagt ggtgacagag ctgcggcgca gattcttttt cattgagcat cactctcagg
    26041 cgcggcattg attgcaggcc gtgctggcga tgcgtctctg aacaggcggc ctcacaggtg
    26101 tggcagccga tacagagcgt ggagtcggca attacaaaac gattcaccag gcattcctca
    26161 ggtgattgtc atttttgacg aaaacatgcc gatgaaatgt cattttcgac actcatcgac
    26221 acgcccatcc ccaaacaggc gtaacgcctg caaaacgggc aaagcctcag ctcatgctgc
    26281 cgggctttgt ccctttacca gttggcttaa attcaccggc acattgtttt caatggcggt
    26341 gtataagctg tcgtaaacgc cagcgatttt ttcgagatag tgttccagaa cctgttcgcg
    26401 atcgcggatg ttgaccttgc catcaatcat gccgtcaatg ctcagttgcg cctgcgcaat
    26461 ccgctgtttg tcttcgccat cttttgtttc gacgtaatac tcgatggtgt ggctgttaaa
    26521 gccatccgcc agggtgacgt aaatacgaaa atgttcaaaa aggacgaaac agagttcttt
    26581 gtccggcgcg cagctcatgg cgtgatgcag gcgcgctttc gataacgtga tcccctgaac
    26641 cagcgaattg cagtagatgt gccactggtc ctgtaggcga cgatgccgct gtgcgatgta
    26701 atcggctttc tcgcttattt cccaaatagt cattgtcagg ttactcgttt aacagagatg
    26761 ccagctttaa gcattttttg tgccaacttt taatttattg ttattaaaga gatttttaag
    26821 ctaaagatga atttcgtcgc cgtgtcgacg tgtcatttcg acatcatcga cattattcac
    26881 cgcagggata atcaacactg gcgcaattat tgcttgtagc tggcaatagt taatgggagg
    26941 cgatatgcac gaaataaccc tctgccaacg ggcactggaa ttgatcgaac agcaggccgc
    27001 aaaacacggc gcaaaacgcg taactggggt ctggctcaaa attggcgcat tttcttgtgt
    27061 cgaaaccagc tctcttgcct tttgttttga tctggtttgc cgcggcagcg tggcggaagg
    27121 ttgtaaactg cacctcgaag aacaagaggc cgaatgctgg tgtgaaacat gccaacagta
    27181 tgtgacgcta ctgacccagc gcgtccgccg ctgtccacag tgtcatggtg acatgctaca
    27241 gattgtggca gacgacggtt tacagattcg gcggatagaa atagaccagg agtgagcgat
    27301 gtgtacaaca tgcggttgcg gtgaaggcaa cctgtatatc gagggtgatg aacataaccc
    27361 tcattccgcg tttcgtagcg cgccatttgc cccggcggca cgcccgcaga tgaaaatcac
    27421 cggcattaaa gcgcctgaat ttacccccag ccagactgaa gaaggcgacc tgcattacgg
    27481 tcatggcgaa gcgggcactc acacaccggg catgagccag cgtcggatgc tggaagtcga
    27541 aattgacgtg ctggacaaaa acaaccgtct ggctgaacgc aaccgcgcgc gctttgctgc
    27601 ccgcaagcaa ctggtgctca acctggtttc cagccctggt tccggtaaaa ccaccctgct
    27661 gacggaaacc ttaatgcgcc tgaaagacag cgttccgtgc gcggttattg aaggcgacca
    27721 gcaaaccgtg aacgatgccg cacgcattcg cgctaccggt acaccagcga ttcaggtgaa
    27781 caccggtaaa ggctgccatc ttgacgcaca gatgattgcc gacgccgcac cgcgtctgcc
    27841 actggacgat aacggtattc tgtttatcga aaacgttggc aacctcgtat gcccggccag
    27901 cttcgatctc ggtgaaaaac acaaagtagc ggtgctttcc gttaccgaag gtgaagacaa
    27961 accgctgaaa tatccgcata tgtttgccgc cgcctcgctg atgctgctca acaaagttga
    28021 cctgttgccg tatctcaact ttgacgttga gaagtgcatc gcctgcgccc gcgaagtcaa
    28081 tccagaaatt gaaatcatcc ttatttccgc caccagcggc gaagggatgg accagtggct
    28141 gaactggctg gagacacagc gatgtgcata ggcgttcccg gccagatccg caccattgac
    28201 ggcaaccagg cgaaagtcga cgtctgcggc attcagcgcg atgtcgattt aacgttagtc
    28261 ggcagctgcg atgaaaacgg tcagccgcgc gtgggccagt gggtactggt tcacgttggc
    28321 tttgccatga gcgtaattaa tgaagccgaa gcacgcgaca ctctcgacgc cttacaaaac
    28381 atgtttgacg ttgagccgga tgtcggcgcg ctgttgtatg gcgaggaaaa ataatgcgtt
    28441 ttgttgatga atatcgcgcg ccggaacagg tgatgcagtt aattgagcat ctgcgcgaac
    28501 gtgcttcaca tctctcttac accgccgaac gccctctgcg gattatggaa gtgtgtggcg
    28561 gtcataccca cgccattttt aaattcggcc tcgaccagtt actgcctgaa aacgttgagt
    28621 ttatccacgg tccgggttgc ccggtgtgcg tactgccgat gggtagaatc gacacctgcg
    28681 tggagattgc cagccatccg gaagtcatct tctgtacctt tggcgacgcc atgcgcgtac
    28741 cagggaaaca gggatcgctg ttgcaggcaa aagcacgcgg tgccgatgtg cgcatcgttt
    28801 actcgccgat ggatgcgttg aaactggcgc aggagaatcc aacccgcaaa gtggtgttct
    28861 tcggcttagg ttttgaaacc accatgccga ccaccgccat cactctgcaa caggcgaaag
    28921 cgcgtgatgt gcagaatttt tacttcttct gccagcacat tacgcttatc ccgacgctgc
    28981 gcagtttgct ggaacagccg gataacggta tcgacgcgtt cctcgcgccg ggccacgtta
    29041 gtatggttat cggcactgat gcctataatt ttatcgccag cgattttcat cgtccgctgg
    29101 tggtcgctgg tttcgaaccc cttgatctac tgcaaggcgt ggtcatgctg gtggagcaga
    29161 aaatagcggc ccacagcaag gtagagaatc agtatcgtcg ggtggtaccg gatgccggta
    29221 acctactggc gcaacaggcg attgccgatg tgttctgtgt caacggcgac agcgaatggc
    29281 gcggcttagg cgtgattgaa tcttctggcg tgcacctgac gccggattat caacgattcg
    29341 atgccgaagc acatttccgc ccggcaccgc agcaggtctg cgatgacccg cgcgcgcgtt
    29401 gtggtgaggt attaacgggc aaatgtaagc cgcatcaatg cccgctgttt ggtaacacct
    29461 gtaatcctca aaccgcattt ggtgcactga tggtctcctc cgaaggagcg tgcgccgcgt
    29521 ggtatcagta tcgtcagcag gagagtgaag cgtgaataat atccaactcg cccacggcag
    29581 cggcagccag gcgatgcagc aattaatcaa cagcctgttt atggaagcct ttgccaaccc
    29641 gtggctggca gagcaggaag atcaggcacg tcttgatctg gcgcagctgg tagcggaagg
    29701 cgaccgtctg gcgttctcca ccgacagtta cgtcattgac ccgctgttct ttcctggcgg
    29761 taatatcggc aagctggcga tttgcggcac cgcgaatgac gttgcgggca gtggcgctat
    29821 tccgcgctat ctctcctgtg gctttatcct cgaagaagga ttgccgatgg agacactgaa
    29881 agccgtagtg accagcatgg cagaaaccgc ccgcacggca ggcattgcca tcgttactgg
    29941 cgatactaaa gtggtgcagc gcggcgcggc agataaactg tttatcaaca ccgctggcat
    30001 gggcgcaatt ccggcgaata ttcactgggg cgcacagacg ctaaccgcag gcgatgtatt
    30061 gctggttagc ggtacactcg gcgaccacgg ggcgactatc cttaacctgc gtgagcagct
    30121 ggggctggat ggcgaactgg tcagcgactg cgcggtgctg acgccgctta ttcagacgct
    30181 gcgtgacatt cccggcgtga aagcgctgcg tgatgccacc cgtggtggtg taaacgcggt
    30241 ggttcatgag ttcgcggcag cctgcggttg tggtattgaa atttctgaat cagcgctgcc
    30301 ggttaaacct gccgtgcgcg gcgtttgcga attgctggga ctggacgccc tgaactttgc
    30361 caacgaaggc aaactggtga tcgccgttga acgcaacgcg gcagagcaag tgctggcagc
    30421 gttacattcc catccactgg ggaaagacgc ggcgctgatt ggtgaagtgg tggaacgtaa
    30481 aggtgttcgt cttgccggtc tgtatggcgt gaaacgaacc ctcgatttac cacacgccga
    30541 accgcttccg cgtatatgct aataaaattc taaatctcct atagttagtc aatgaccttt
    30601 tgcaccgctt tgcggtgctt tcctggaaga acaaaatgtc atatacaccg atgagtgatc
    30661 tcggacagca agggttgttc gacatcactc ggacactatt gcagcagccc gatctggcct
    30721 cgctgtgtga ggctctttcg caactggtaa agcgttctgc gctcgccgac aacgcggcta
    30781 ttgtgttgtg gcaagcgcag actcaacgtg cgtcttatta cgcgtcgcgt gaaaaagaca
    30841 cccccattaa atatgaagac gaaactgttc tggcacacgg tccggtacgc agcattttgt
    30901 cgcgccctga tacgctgcat tgcagttacg aagaattttg tgaaacctgg ccgcagctgg
    30961 ccgcaggtgg gctatacccc aaatttggtc actattgcct gatgccactg gcggcgggag
    31021 ggcatatttt tggtggctgt gaatttattc gttatgacga gcgtccctgg agcgaaaaag
    31081 agttcaatcg tctgcaaaca tttacgcaga tcgtttctgt cgtcaccgaa caaatccaga
    31141 gccgcgtcgt caacaatgtc gactatgagt tgttatgccg ggagcgcgat aacttccgca
    31201 tcctggttgc cattaccaac gcggtgcttt cccgcctgga tatggacgaa ctggtcagcg
    31261 aagtcgccaa agaaatccat tactatttcg acattgacga tatcagcatt gtcttacgca
    31321 gccaccgtaa aaacaaactc aacatctact ccactcacta tcttgataaa caacatcccg
    31381 cccacgaaca gagcgaagtc gatgaagccg gaaccctcac cgaacgcgtg ttcaaaagta
    31441 aagagatgct gctgatcaat ctctacgagc gggacgattt agccccctat gaacgcatgt
    31501 tgttcgacac ctggggcaac cagattcaaa ccttgtgcct gttaccgctg atgtctggcg
    31561 acaccatgct gggcgtgctg aaactggcgc aatgcgaaga gaaagtgttt accactacca
    31621 atctgaattt gctgcgccag attgccgaac gtgtggcaat cgctgtcgat aacgccctcg
    31681 cctatcagga aatccatcgt ctgaaagaac ggctggttga tgaaaacctc gccctgaccg
    31741 agcagctcaa caatgttgat agtgaatttg gcgagattat tggccgcagc gaagccatgt
    31801 acagcgtgct taaacaagtt gaaatggtgg cgcaaagtga cagtaccgtg ctgatcctcg
    31861 gtgaaactgg ctcgggtaaa gagctgattg cccgtgcgat ccataatctc agtgggcgta
    31921 ataatcgccg catggtcaaa atgaactgcg cggcgatgcc tgccggattg ctggaaagcg
    31981 atctgtttgg tcatgagcgt ggggctttta ccggtgccaa cgcccagcgc attggtcgtt
    32041 ttgaactggc ggataaaagc tctctgttcc tcgacgaagt gggcgatatg ccactggagt
    32101 tacagccgaa gttgctgcgt gtattgcagg aacaggagtt tgaacgtctc ggcagcaaca
    32161 aaatcattca gacggacgta cgtttaattg ccgcgactaa ccgcgatctg aaaaaaatgg
    32221 tcgccgaccg agagttccgt agcgatctct attaccgcct gaacgtattc ccgattcacc
    32281 tgccgccact acgcgagcgt ccggaagata
//
LOCUS       DAJTUI010000040        30997 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTUI010000040 DAJTUI010000000
VERSION     DAJTUI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.040"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            211..1869
                     /locus_tag="OO464_002004"
     CDS             211..1869
                     /locus_tag="OO464_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6227161.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1866..2822)
                     /locus_tag="OO464_002005"
     CDS             complement(1866..2822)
                     /locus_tag="OO464_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR6227162.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2973..4088
                     /gene="macA"
                     /locus_tag="OO464_002006"
     CDS             2973..4088
                     /gene="macA"
                     /locus_tag="OO464_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6227163.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4085..6031
                     /gene="macB"
                     /locus_tag="OO464_002007"
     CDS             4085..6031
                     /gene="macB"
                     /locus_tag="OO464_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6227164.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6104..6328)
                     /gene="cspD"
                     /locus_tag="OO464_002008"
     CDS             complement(6104..6328)
                     /gene="cspD"
                     /locus_tag="OO464_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6227165.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6651..6971
                     /gene="clpS"
                     /locus_tag="OO464_002009"
     CDS             6651..6971
                     /gene="clpS"
                     /locus_tag="OO464_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6227166.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7002..9278
                     /gene="clpA"
                     /locus_tag="OO464_002010"
     CDS             7002..9278
                     /gene="clpA"
                     /locus_tag="OO464_002010"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6227167.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9681..9768)
                     /locus_tag="OO464_002011"
     tRNA            complement(9681..9768)
                     /locus_tag="OO464_002011"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9732..9734),aa:Ser,seq:gga)
     gene            complement(10022..10240)
                     /gene="infA"
                     /locus_tag="OO464_002012"
     CDS             complement(10022..10240)
                     /gene="infA"
                     /locus_tag="OO464_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6227168.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10525..11229)
                     /gene="aat"
                     /locus_tag="OO464_002013"
     CDS             complement(10525..11229)
                     /gene="aat"
                     /locus_tag="OO464_002013"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6227169.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11271..12992)
                     /gene="cydC"
                     /locus_tag="OO464_002014"
     CDS             complement(11271..12992)
                     /gene="cydC"
                     /locus_tag="OO464_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6227170.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12993..14759)
                     /gene="cydD"
                     /locus_tag="OO464_002015"
     CDS             complement(12993..14759)
                     /gene="cydD"
                     /locus_tag="OO464_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6227171.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14882..15847)
                     /gene="trxB"
                     /locus_tag="OO464_002016"
     CDS             complement(14882..15847)
                     /gene="trxB"
                     /locus_tag="OO464_002016"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6227172.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16391..16885
                     /gene="lrp"
                     /locus_tag="OO464_002017"
     CDS             16391..16885
                     /gene="lrp"
                     /locus_tag="OO464_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6227173.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17020..21048
                     /gene="ftsK"
                     /locus_tag="OO464_002018"
     CDS             17020..21048
                     /gene="ftsK"
                     /locus_tag="OO464_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6227174.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21203..21814
                     /gene="lolA"
                     /locus_tag="OO464_002019"
     CDS             21203..21814
                     /gene="lolA"
                     /locus_tag="OO464_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6227175.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21825..23168
                     /gene="rarA"
                     /locus_tag="OO464_002020"
                     /gene_synonym="mgsA"
     CDS             21825..23168
                     /gene="rarA"
                     /locus_tag="OO464_002020"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6227176.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23259..24551
                     /gene="serS"
                     /locus_tag="OO464_002021"
     CDS             23259..24551
                     /gene="serS"
                     /locus_tag="OO464_002021"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6227177.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24791..27234
                     /gene="dmsA"
                     /locus_tag="OO464_002022"
                     /pseudo
     CDS             24791..27234
                     /gene="dmsA"
                     /locus_tag="OO464_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27245..27862
                     /gene="dmsB"
                     /locus_tag="OO464_002023"
     CDS             27245..27862
                     /gene="dmsB"
                     /locus_tag="OO464_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6227178.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27864..28655
                     /locus_tag="OO464_002024"
     CDS             27864..28655
                     /locus_tag="OO464_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6227179.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28691..29317)
                     /locus_tag="OO464_002025"
     CDS             complement(28691..29317)
                     /locus_tag="OO464_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6227180.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29632..30780
                     /locus_tag="OO464_002026"
     CDS             29632..30780
                     /locus_tag="OO464_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6227181.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7627 a         7616 c         8328 g         7426 t
ORIGIN      
        1 atcctttagg cgtaataaaa aataatttat catgctaatt atttgatttt gttgtttttg
       61 cgggcttatc agcaagaggg agtataacgc gattattcgc tcatttttca gacaattgcc
      121 atgcttaaat gtgatgtcat cacgtattag caaggccttt cccgttatac tgcgagcgta
      181 aaggataagt cacatatttc tggaggggat atgattcttg agcgcgttga aattgtgggt
      241 tttcgcggta tcaaccgttt gtcgttgatg ctcgaacaaa acaacgtcct gattggggag
      301 aacgcgtggg gtaaatccag cttgctggac gccttaaccc tgctgctatc gccagaatca
      361 gatctctacc attttgagcg cgacgatttc tggttcccgc cgggagatat caacgggcga
      421 gaacatcatc tgcatattat tttgaccttc cgcgaatcgc tgccaggccg acatcgggtt
      481 cgccgttatc ggccgctgga agcgtgctgg acgccatgca ccgatggcta tcaccgtatt
      541 ttttatcgtc tggaagggga gagtgcggaa gacggcagcg tgatgacact gcgcagtttt
      601 ctcgataaag acggacatcc gattgatgtt gaggatatta acgatcaggc acgccatctg
      661 gtgcgtttaa tgccggtgct gcgcttgcgt gatgcccgtt ttatgcgccg tattcgtaac
      721 ggcacggtgc caaatgtccc caatgtggaa gtcaccgcgc gccagctcga tttcctcgcc
      781 cgtgagttgt cctcacatcc gcaaaatctc tctgatggac agattcgtca gggactttcc
      841 gcaatggtgc agttgcttga gcattatttc tctgagcagg gggctggaca ggcgcgatat
      901 cgtttaatgc ggcggcgggc cagcaatgag caacgaagct ggcgctatct ggacatcatc
      961 aaccggatga ttgaccgacc tggagggcgc tcgtatcggg ttattttgct cggcctgttt
     1021 gctactttat tgcaggcaaa aggcacattg cgactggata aagacgcgcg tccgttattg
     1081 ctaattgaag atccagaaac ccgcctgcat ccgattatgc tttccgttgc ctggcatctg
     1141 ttgaatcttt tgcctttaca gcgtattgct accaccaact ctggcgagct gctttcgtta
     1201 actccagtgg aacatgtttg ccgcctggtg cgtgaatctt ctcgtgttgc cgcctggcgt
     1261 ctggggccaa gtggcttgag caccgaagac agccgccgca tctcttttca cattcgtttt
     1321 aatcgtccgt catcgctgtt tgctcgctgc tggttgctgg tggaagggga aacggaaacc
     1381 tgggttatca atgaactggc gcgtcagtgc ggacatcatt ttgatgccga agggatcaag
     1441 gtcattgagt ttgcccagtc cgggctaaag ccactggtta aatttgcccg ccgaatgggg
     1501 attgaatggc atgtactggt cgatggcgat gaagcaggga agaaatatgc cgctacggta
     1561 cgcagcctgt tgaataacga tcgggaagcc gaacgagaac atttaacggc gttaccggcg
     1621 ctggatatgg aacattttat gtatcgccag ggattttccg atgtgttcca ccgcgtggcg
     1681 caaatcccgg aaaatgtacc gatgaatcta cgcaaaatta tctcgaaagc gatccatcgc
     1741 tcttccaaac ccgatcttgc cattgaagtg gcaatggagg caggacgtcg tggtgtggac
     1801 tccgtaccaa cgctgctgaa aaaaatgttc tcacgcgtgc tgtggctggc gcgcggtcgc
     1861 gcggattaac cgcgaaacat cgtggccatt tgtggctgaa tagcgtcgag catctcatag
     1921 cgccgacggt attcagcccg ttttttactg gcgatttcgg caatctcttt tcgtgctatc
     1981 tgtgctggaa ggcggtaatg gcgttcagca tcacatacgc cgccaaccga ctcccagaac
     2041 gcgttgtaat cagcatggat cttgccttct ttatcgcgat aacgcaggct gcggtaaata
     2101 tgcgtttcat tgctgacggc aataatctgc tctacctgca aacgttgggc aaacagacag
     2161 gccgcttcca tcacgaggcg tttgggaaat agcccgtggc aggctttcgt cgcattctgg
     2221 atttcctgat gtggaatttc ccattttgcg ccttgcagtc cgccaataaa catcgttctt
     2281 ttcccctgat attcacacag ggtaaacgtg atctctgcca gaggaatacc ttcgctgttg
     2341 cggaacagga ttgtgctgtc accttcttta tccattgaga tcatcatggt cagctcaagc
     2401 gtgaactgct caccgttttt gccttccagt ttagccagtt gcagcccggg ggtattcaaa
     2461 tataagctga attcttccgc cgacatacac ccgcggagta acgcataatg gtaacgtaac
     2521 gcctccagca attgcttacg gctaagattc gcggcaaggt aagggcgatg cagacgcaca
     2581 ggcagtcgcg gctggcgcgt taacaacaca ttgagattag gccagtggga aagttcgttc
     2641 atccactcaa cgcttaaacg cggcataatc aacgagcgca gcaaaaattt ctggcgaaaa
     2701 ctacagcgat gccagaattt acccggccga cactgtccac gtgccagact aagaaaaagt
     2761 gacaggctgc tgagagattc agatggcgta aaggtccgtt cagttagctg cgacatattc
     2821 atgaaatcaa tggttataca tgacgtcgat ttcaccattg cgtatcttaa ccaaacatca
     2881 atagtgtgat tactaacgta aattttaggg ttttgttgat atttcgttga agttaatgac
     2941 ccggattggc atatggagta ttcagaatat ttatgaaaaa gcggaaaacc gtgaagaagc
     3001 gttacgttat tgcgctggtg atagtcatcg ccggactgat tacgttatgg agaattctta
     3061 acgcacccgt gccgacttat cagacactga ttgtgcgccc cggtgattta cagcaaagcg
     3121 tgctggcgac cggaaagctg gacgcgctgc gtaaggttga cgtgggcgcg caggtcagcg
     3181 gtcagttgaa aacgctgtcg gtggcgattg gcgataaagt aaaaaaagac cagcttttag
     3241 gggtcattga tcctgaacag gctgaaaacc agatcaagga ggtcgaagca acgctgatgg
     3301 agctacgcgc gcagcggcag caggcggaag cggagctgaa actggcgcgg gtgacgtatt
     3361 cccgtcagca acgtctggca caaacgcaag cggtttcact gcaggatctc gataccgccg
     3421 cgacggagat ggctgtgaaa caggcgcaaa ttggcaccat tgacgcgcaa atcaagcgca
     3481 atcaggcctc tctcgatacg gctaaaacca atctcgatta cacccgtatc gttgccccga
     3541 tggctgggga agtgacgcaa atcaccactc tgcaaggcca gacggtgatt gccgcacagc
     3601 aagcaccgaa cattctgacg ctggcagata tgagcactat gctggtaaaa gcgcaggttt
     3661 ctgaagcgga tgtaatccac ctgaagccgg ggcaaaaagc ctggtttacg gtgcttggcg
     3721 atccactgac gcgctacgag gggcaaatca aggatgtgct accgacgccg gaaaaggtaa
     3781 acgacgctat tttctattac gcccgttttg aagtacccaa ccccaatggt ttgctgcggc
     3841 tggatatgac cgcgcaagtg catattcagc tcaccgatgt gaaaaatgtg ttgacgatcc
     3901 ctctgtcggc gttaggcgat ccggttggcg ataatcgtta taaagtcaaa ttgttgcgta
     3961 atggtgaaac acgcgagcgt gaagtgacga ttggcgcacg taacgatacc gatgttgaga
     4021 ttgtcaaagg gctggaagcg ggcgatgaag tggtgattgg tgaggccaaa ccaggagctg
     4081 cacaatgacg cctttgctcg aattaaagga tattcgtcgc agctatcctg ccggtgatga
     4141 gcaggttgag gtgctgaagg gcatcagcct caatatttat gcgggtgaga tggtcgcgat
     4201 tgttggcgct tcgggttccg gtaaatcgac cctgatgaat attctcggct gcctggataa
     4261 ggccaccagc ggcacctatc gcgtagccgg tcaggatatt gccacgctgg acgccgatgc
     4321 gctggcgcaa ctgcgccgcg agcatttcgg ctttattttc cagcgttacc atttgcttcc
     4381 gcatttaacc gtcgagcaga acgttgaagt acccgccgtc tatgccggtc ttgagcggaa
     4441 acagcgactg cttcgtgccc aggagttgct gcaacggctg gggctggaag accgtacaga
     4501 gtattatccg gcacagcttt cgggtggtca gcaacagcgc gtcagcatcg cgcgggcatt
     4561 gatgaacggc ggtcaggtaa ttcttgccga tgaaccgacc ggcgcactgg acagccattc
     4621 tggcgaagag gtgatggcga tcctgcatca gctgcgcgat cgcgggcata cggtgattat
     4681 cgtcacccac gatccgcagg tcgctgctca ggccgagcgg gtgatcgaaa ttcgcgacgg
     4741 cgaaattgtg cgcaatcctc ccgccattga aaaagtgaat gttgctggcg ggacggagcc
     4801 tgttgtcaat acagtgtctg gctggcggca gtttgtcagc ggttttaacg aggcgctgac
     4861 gatggcatgg cgggcgctgg cagcgaataa aatgcgtact ttactgacca tgctggggat
     4921 tattatcggt attgcgtcgg tggtttccat tgtcgtggtg ggtgacgccg ccaaacaaat
     4981 ggtgctggcg gatattcgtt ctattggtac gaatactatt gatgtctatc ccgggaaaga
     5041 ttttggcgat gacgatccgc aatatcagca ggcgctgaag tacgacgact taatcgccat
     5101 ccaaaaacaa ccgtgggtcg cctcagccac acctgccgtc tcgcaaaacc tgcgcctgcg
     5161 ttataacaat gttgatgttg ctgccagtgc caatggcgtg agcggcgatt attttaatgt
     5221 ctatggcatg accttcagtg aaggaaacac ctttaatcag gagcagctga acggtcgtgc
     5281 gcaggtcgtg gttctcgaca gtaatactcg ccgccagctt ttcccccata aagcagatgt
     5341 ggttggcgag gtgattctgg tcggcaatat gcccgccaga gtcattggtg tggcggaaga
     5401 aaaacagtcg atgtttggta gcagtaaagt gctgcgtgtc tggctacctt acagcacgat
     5461 gtccgggcga gttatgggcc agtcgtggct taactccatt actgtcaggg tgaaagaagg
     5521 atttgacagc gccgaggcgg aacagcaact cacgcgttta ctttcactgc gccacggtaa
     5581 gaaggatttc tttacctgga acatggacgg cgtcttgaaa actgttgaaa agaccacacg
     5641 tactttacaa ctgtttctga cgctggtggc ggtgatttcg ctggtggtgg gcggtattgg
     5701 tgtaatgaat attatgctgg tgtcagtgac cgagcggacg cgggaaattg gcattcgcat
     5761 ggctgtaggt gcgcgagcaa gcgatgtttt gcaacagttc ctgatcgaag ccgtactggt
     5821 ttgcctggtc ggtggcgcgt tgggaataac actgtcactg ttaattgctt tcaccttgca
     5881 gcttttctta cccggctggg agattggttt ttcaccgttg gcgctgctgc tggcgtttct
     5941 ctgctcgacg gccaccggga ttttatttgg ctggttaccc gcacgaaatg cggcacgact
     6001 ggatccagta gatgctctgg cacgggagta atttttgaga taaaaatgcc agccgatcgg
     6061 gctggcattt tgcctttagg atgtacacaa tgagacagaa gagctatgcg actgccgctt
     6121 ctacttcgac gggcacaata acactggcgt gattgccttt tggcccctgg tggacatcaa
     6181 actgaacgga ttgtccagct tttagcgttc tgtaaccatc catctgaatg gtggaataat
     6241 gagcgaaaat atcttcgccg ccgccttcag ggcagatgaa accaaaccct ttggcattgt
     6301 tgaaccactt aacagtaccc ttttccatgc ttcgacatcc ttcgcaaatc ttatacaagt
     6361 aagatggaat aaaccggggt cagagagggg gctgttcaaa acctcgccaa ctctagaaat
     6421 acaatttaga gaattagggc gagccgtcaa gcatttgaca ggggccaggg ggcaggtatg
     6481 aatcaaaaat ttgaagcagt taacgctatt gacaggaatg tgacagatgt cgctgatgcc
     6541 aacgatagat gatagttatc tatcatgtgg agtagattgg tcaggcaaat aagctcttgt
     6601 cagcggcagg gcgttctgcc gataaccgta accgaagatg ataactgaca atgggtaaaa
     6661 cgaacgactg gctggacttt gatcaactgg cggaagaaaa agttcgcgac gcgctaaaac
     6721 cgccatctat gtataaagtg atattagtca atgatgatta cactccgatg gagtttgtta
     6781 ttgacgtgtt acaaaaattc ttttcttatg atgtagaacg tgcaacgcaa ttgatgctcg
     6841 ctgttcacta ccaggggaag gccatttgcg gagtctttac cgccgaggtt gcagaaacca
     6901 aagtggcgat ggtgaacaag tacgcgaggg agaatgagca tccattgctg tgtacgctag
     6961 aaaaagcctg aatgcaggca taaaaattgg gggaggtgcc tatgctcaat caagaactgg
     7021 aactcagttt aaatatggct ttcgccagag cgcgcgagca ccgtcatgag tttatgaccg
     7081 tcgagcactt gttactggcg ctgctcagta acccatctgc ccgggaggcg ctggaagcgt
     7141 gttctgtgga tttggttgcg ctccgtcagg aactggaagc ctttattgaa caaaccacac
     7201 ccgttctgcc tgccagtgaa gaggagcgcg acacacagcc gacgctgagt tttcagcgtg
     7261 tactgcaacg tgcggtcttc catgtccagt cctccggtcg caatgaggtt accggtgcaa
     7321 acgttctggt cgctatcttc agcgaacagg agtcgcaggc ggcatatctg ttgcgtaaac
     7381 atgaagtcag ccgtctcgat gtggtgaact ttatctctca tggcacgcgt aaagacgagc
     7441 cgacacagtc ttctgatcct ggcagccagc caaacagcga agaacaagct ggtggggagg
     7501 aacgtatgga gaatttcacg acgaacctga accagcttgc gcgtgtgggc ggaatcgacc
     7561 cactaattgg tcgtgagaaa gagctggagc gtgctattca ggttctctgc cgtcgccgta
     7621 aaaacaaccc gctgctggtg ggggaatctg gtgtcggtaa aaccgcgatt gcagaaggtc
     7681 ttgcctggcg aattgttcag ggtgatgtgc cggaagtgat ggctgactgt acgatttact
     7741 ctctcgatat cggttctctg ttagcgggca caaaatatcg cggcgacttt gaaaaacgtt
     7801 ttaaagcgtt gctcaagcag ctggagcagg acactaacag catcctgttt attgatgaga
     7861 tccacaccat tatcggtgcg ggtgcagcgt ctggtggtca ggtcgatgcg gctaacctaa
     7921 tcaaaccgtt gctctccagc ggtaaaattc gcgtaattgg ttcgacaacc tatcaggagt
     7981 tcagcaacat tttcgagaaa gaccgtgctc tggcgcgtcg cttccagaaa attgatatta
     8041 ctgaaccgtc gatcgaagaa actgttcaaa tcatcaatgg cctgaaaccg aagtatgaag
     8101 cgcaccacga cgtgcgttat accgcaaaag cggtgcgtgc ggcggtagag ctggcggtga
     8161 aatacattaa cgatcgtcat ctgccggata aagccattga tgttatcgac gaagcaggcg
     8221 ctcgcgcacg tctgatgccg gtaagcaaac gcaagaaaac cgttaatgtg gcggatattg
     8281 agtccgtggt ggcccgtatt gcacgcattc cagagaagag tgtttctcag agtgaccgcg
     8341 ataccctgaa aaaactcagc gatcgcctga aaatgctggt cttcggccag gataaagcca
     8401 ttgaggcgct gactgaagcc attaagatgg cgcgtgcagg tttaggtcac gaacataaac
     8461 cggtcggttc gttcctgttt gccggtccta ccggggtcgg gaaaacagag gtgacggtac
     8521 agctttcgaa agcgttgggc attgagcttc tgcgctttga tatgtccgag tatatggaac
     8581 gccataccgt cagccgtctg attggtgcgc ctccgggata cgttggtttt gatcagggcg
     8641 gtttgctgac cgatgcggta atcaagcatc cacatgcggt gctgctgctg gacgaaatcg
     8701 agaaagcgca tccggacgtg ttcaatattc tgttgcaggt gatggacaac ggtacgctga
     8761 ccgataacaa cggacgcaaa gcggacttcc gtaacgtggt gctggtgatg accactaacg
     8821 ccggggtacg ggaaactgag cgcaaatcca ttggtcttat ccaccaggat aacagcaccg
     8881 atgcgatgga agagatcaag aagatcttta caccggaatt ccgtaaccgt ctcgacaaca
     8941 ttatctggtt cgatcatctg tcaactgacg tgatccatca ggtggtggat aaattcatcg
     9001 tcgagttgca ggttcagctg gatcagaaag gtgtttctct ggaagtgagc caggaagcgc
     9061 gtaactggct ggccgagaaa ggttacgacc gggcaatggg cgcacgtccg atggcgcgtg
     9121 tcatccagga caacctgaaa aaaccgctcg ccaacgaact gctgtttggt tcgctggtgg
     9181 acggcggtca ggtgacggtt gcgctggata aagagaaaaa tgagctgact tacggattcc
     9241 agagtgcaca aaagcacaag gcggaagcag cgcattaatc tgattgtcag gtaggttggt
     9301 gaagtccgta atctcgtcag gggttacgga ctttttattt atggggtgga acggtgaaaa
     9361 ccctattttt ggaggtgaag gtaagttgtt gataattagt gctgctggaa ggtaaggata
     9421 aaaaagggtg ctgcaggaga atgggatggt tttgctttat taacaacggg ctaaacgtgt
     9481 agtatttgag ttcactgccg tacaggcagc ttagaaatcg acggggtgcg gtaaaacctt
     9541 tgcgaacgcg ttcactgccg tacaggcagc ttagaaattc acaggtaaca tactccaccc
     9601 gcccaccatg ttcactgccg tacagacaga taaaatgcga aaaaaaagct cgcactttcg
     9661 tacgagctct tctttaaata tggcggtgag ggggggattc gaacccccga tacgttgccg
     9721 tatacacact ttccaggcgt gctccttcag ccactcggac acctcaccaa attgttttgc
     9781 taccaaacct catgggtggc aacggggcgc tactataggg agttggagta aaacggtcaa
     9841 gaagaatttt aatgataatt attgtttgct catactgtaa acaacttgtg cagtatatct
     9901 acatcgagac aagttatgga cttatacttc caaagtactt catacatatc acaaaataaa
     9961 aaggccggtt aaaccgacct tttactcgtt ctttctcttc gcccatcagg cggtaaaaca
    10021 atcagcgact acggaagaca atgcggcctt tgctcaggtc gtacggggtc agttcaacag
    10081 tcactttgtc gcccgtcagg atgcggatgt agtttttgcg cattttaccg gagatgtgtg
    10141 cagtaaccac gtgaccgttt tctaactcta cgcggaacat ggtattaggc aacgtttcaa
    10201 gaacggtacc ttgcatttca atattgtctt ctttggccat ctaatcctct ggggtatcac
    10261 taccgtaatt tgaaccggca agataatgcc gaagttctgt aaataagtaa agatttgcgc
    10321 gctaaatcgc aacaaacagg ttcggcacat tactccgaaa acacacggct aagccgcacc
    10381 aaaagcgcaa cgtataaggg agcggtgaga taaacgatgg gcgttacctg acgcgaaaaa
    10441 ttccttatcg gcagcggggt aatgagcgta accaactctg cgaccgcaat tataacactc
    10501 tggggagaaa tgtgccgaaa acattcattc ttgtggtgaa aacaagcacc gtggtaccca
    10561 gaaattattc ggcaatcgtc cgaggcgcat ttgattgaga taattaaggt aatcccggcg
    10621 gggaatttcg caggcaccaa gcgatgctgt gtgatcgtta aggacctggc agtcgataag
    10681 cttaccgcca tgaccgataa attcctcaca gaataccaga agcgccgttt tagacgcatt
    10741 ttccatccgg ctgaacatgg actcgccaca aaatagcgtt ccctgggcca cgccgtacat
    10801 accgccgaca agctcatctt cacgccagac ttcaatggag tgggcatgac cgagttcgtg
    10861 aaggcgatgg taggcttcga ccacgccacg cgtgatccag gttccttctt cgcgatcgct
    10921 ggcacagcct tcaatgacct gaccaaaagc gtaattcatc gtgacacgat agggcgagcg
    10981 tttatgaaat cgcttcatac tacggctgat atgcagtgat tctggccata gcaccgcgcg
    11041 gggatcgggc gaccaccaga ggatggggtc gcctggagaa aaccacggaa aaataccacg
    11101 ctggtaagcc attaacaggc gcgcagggct aagatcgccc ccaagtgcca gcaggccgtt
    11161 aggctcacgt aatgcgcctt ccggggaagg gaaggctatt gaatggcgag aaagctgaac
    11221 caagcgcatg accgcaaaac tccacgcaag tcggatcgtt caataatagc ttacaaaccc
    11281 tgcttgaact ggtaataacg cccctgtctg gcaagcagtt ctgcgtgagt accttgctca
    11341 ataatttgcc cgttgtccat cactattatt tgttggaaac gagagagtcc gcgaagtcga
    11401 tgggtgacca ttaacaccgt tttctcacgc atcatttttg caagcaattc aaggatctgg
    11461 ctttcggttg tggcatctaa gccttcggta ggttcatcca gcaacaccag tggcgcatca
    11521 tgtaacagcg tacgggcgat agccagacgg cgcagttcac caccggagag ctggcgtccg
    11581 ccttcaccta accaactgtt gagacctgca tcctcgagca gcttctccag accaacgcga
    11641 cgcaagatct ccgacagagc ctcatcacta ctgccaggcg aggcgagtaa cagattatca
    11701 cgcagtgtgg cgctaaacag atgcactcgc tgaggaacaa cgctgatggt ctgtcgtaga
    11761 gccgcttcat tcaggctggc tatggggcta tcgttaagca aaatctcgcc ctgttgcggg
    11821 tcccatgcgc gggtcagcag ttgtaacagt gttgatttgc cgcatccggt tcgcccgaga
    11881 atcgctatat gttccccggc gtttacctga agagaaatcc ctttaagtgc ctgttgggat
    11941 tgctccggat aagtgaactg aacatcccgt aatgtcagcg aaacgcgatc ggcaacacga
    12001 gtttgggtat cagggaaggt gacctccggt ttttgatccg ttaagtcaga gatacgtacg
    12061 gcagaggcaa tgacttgccc cagatgctga aatgcacccg ttactggtgc cagtgcttca
    12121 aacgcggcta acgcacaaaa gacaaacagg gcaattaatg cgccgggttg agcattgccg
    12181 ccaacgccgc cagacgccat ccacagcatc aggatcaccg ctaacgcgcc aatgagcagc
    12241 attatcgctt gcgacaatgc ggtcagttca gattgacggc gttgcgcttc cagccattga
    12301 atttctgtat tctctagttg cgtgcgataa cgatcgctgg caccaaaaat ggtcagctca
    12361 gcttgccctt gcagccaggc cgtcagttgt tggcgatact gtccgcgaag atgagtcaga
    12421 ttttgcccgg tgctttttcc cgcacgataa aacagcggtg gcatcaggaa aagcgtcagt
    12481 aacataacgc tgcccagcgt aaaggcgagg gtgaaatcaa ggaaacttaa cccgattgtc
    12541 accaccataa tcaccacaaa agcgcccacc agcggcgaga taacgcgcag gtaaagatga
    12601 tcgagcgtat caacatccgc caccacgcgg ttgagcaatt cgccctgacg atagcgcgcc
    12661 agtccggcag gggagagggg caacaatttg ctgaaggtgt aaatgcgcag atgctgcaac
    12721 acgcggaaag tcgcgtcgtg acttaccaga cgttcaaaat aacgcccggc agtacgggtg
    12781 attgcggcac cacgcacgcc cgcagcgggt agcatatagt tgaagctgta cagtccggca
    12841 acccccgcaa ccgctgaggc cgagaggaac cagccggaaa gtgtcaacag accgatactg
    12901 gcgagcagcg tcacaattgc cagcacaata ccaagactta acatccattt atgacgttta
    12961 tacagtgcca gatagggtag caaagcgcgc atttaaatct cctcctgacg atgggccagt
    13021 aatgtggcga atgggccacc agccacactt aattccgcgt aacgtccttg ctcaataatc
    13081 cggccatcct gcataaccca aatgacatcc cagtcagcaa gatcttctaa ctggtgggtg
    13141 accattaacg ttgtctggcg cagagaggcg gcattcagcg cctccattac gcgctgttca
    13201 ctgtgagcat caaggctggc agcgggttca tccaacagta atagcgaaca gggatttagt
    13261 aacgcacggg ccaccgccac gcgctgcgcc tgccccacgg aaaggcgggc agcctggtcg
    13321 ccaacaggtg tatcaacgcc ttgtggcagg agcggtagaa actcgctgac ccaggcgtta
    13381 tccagcgctg cttgtaattc ttgttcgctg gcatcaggtc gcgccagtag tacgttatcc
    13441 cgcaattttg ctgccggtaa ttgtgggttt tgcccaaccc aggagagatg tttacgccat
    13501 gattctggtg ataaatcacg taattctatc ccgttgattc gtaatgatcc ctgatatgag
    13561 agaaaaccag aaagcgcgtt cagcagtgag cttttacctg aaccgctgcg gccaaccaac
    13621 actgcacgtt ggcctgctgg caaagtaaag ttcagcggtc cggccagcgt tttaccttcc
    13681 ggcgacgtga taaacagatc ctcggcctca atggtcagcg gatcggtcaa tgctaattcc
    13741 gcctcaccgc gctgcggatg ggcgagcggg gtttccataa acgttttcag actgtcagct
    13801 gcaccaacag cctgggcttt agcatgataa aacgtaccga gatcgcgtaa tggctggaaa
    13861 aactctggcg caaggatcag ggccagaaaa cctgcagcca gcgtcacccc agtatcgtag
    13921 tgaccaaaat ccagctcgcc gagataggaa aaaccaaagt agaccgccac cagagcaatt
    13981 gacagcgagg taaaaaattc gagaatgccg gaggataaaa acgccagtcg tagcacttcc
    14041 attgtccgtt ggcggaaatc ttccgaagca gaacgaatac tttcaatttc agcttcacca
    14101 cgaccaaaaa tacgcaatgt ttccatgccg cgcaggcgat cgaggaaatg cccacttaag
    14161 cgagcaagag cgagaaagtt acgccggtta gcatcggcag cccccattcc aaccagcgcc
    14221 ataaacaacg gaattagcgg tgctgtgccc agcagaatga gcgccgcagc ccagttagag
    14281 gggaagatcg ccaccacaat cagcaacggc accgacactg ccagcgccat ttgcggcaga
    14341 tagcgtgcat agtaatcatg catatcgtca atttgctcga gtaccagcgt cgcccagctc
    14401 cccgcaggtt taccctgaat ccacgctggc cctgcttgtt gcagacggtc gagaacctga
    14461 cggcggatgg caaagcggat atgctgcccg gcgtgataac ccacccgttc gcgtaaccag
    14521 accacccatg cgcgcaatac aaaggtcaga accagtaacg taaagggaag cagcagggct
    14581 tcacggggaa tattctccat aatcatatgt tgcagaattc gcgccatgaa ccaggcctgg
    14641 gcaatgatca atatgccgct tacaaagccc agcagacgag aaatattcag ccaacgttgg
    14701 gagatgacgc tttgctgttt taaccagcgg gttaactctt tttgacgaga tttattcatt
    14761 gcacgcttag caggtgagtt atcagaatta tttgcagagc aatgttacaa cggggaaaaa
    14821 ataaaggcga cccatagtcg caaggtgtcg ccttctttac ttttgttact gatttgtaaa
    14881 attattttgc gtcagctaaa ccatcgaggt agcgttccgc atcaagtgct gccatgcagc
    14941 cagtaccggc cgaagtaatg gcctggcgat aaatgtgatc catcacgtcg cctgcggcaa
    15001 agacgccagg aatgctggtc tgggtggcat taccatgaat acccgactgt actttgatgt
    15061 agccgttttc cagttccagc tgcccttcga aaatcgcagt attcgggctg tgaccgatag
    15121 caacaaacag accggcaacg tcgagtgact cgatgttatc gctgttttgc gtatcgcgca
    15181 gacgaacgcc agtgacaccc atttgatcgc cggtcacttc ttccagcgta cggttggtgt
    15241 gcagaatgat gttgccgttc tccactttat ccatcagacg cttaataagg attttttccg
    15301 cgcggaaacc gtcacggcgg tgaatcagat gcacttccga agcgatgtta gacagataca
    15361 gcgcctcttc aaccgcggta ttgccgccgc cgatgaccgc aactttctgg ttgcgataga
    15421 agaaaccgtc gcaggttgca caagcagaaa ccccacggcc tttaaaggct tcttcagagg
    15481 gcaggccgag atagcgtgca gaagctccgg tggcaataat cagcgcgtcg caagtgtatt
    15541 cgccgttatc gccattcaga cggaacggac ggttttgcag atccaccttg ttgatatgat
    15601 caaaaatgat ctcagtttca aacttggtgg catgttcgtg catgcgcgcc attaataacg
    15661 gaccggtcag atcgtttgga tcaccaggcc agttttccac ttccgtggtg gtggtcagtt
    15721 ggccgccttt ttccatgccg gtaatcagca caggttgcag gttggcgcgc gccgcgtaga
    15781 cagcagcggt gtatcccgcc gggcctgaac ccaggataag cagtttactg tgtttggtcg
    15841 tgcccatgag atccccatag ttgttggcag acaatgggca ggattgtagg gaatttacag
    15901 acgtaaaaaa agagtatgac gattttgtta acaatttgtg caatcggcag catcgataag
    15961 caggtcaaat tctcccgtca ttatcacctc tgctacttaa atttcccgct ttataagccg
    16021 attaaatgat gaataaacgc ccctgttaat gaatatctgg catgttgtac taaaaatcga
    16081 tgttttgctt tgacaatccc ctggtgtttt gcgaaaacat tcgaggaaga aaaaaacagt
    16141 attcttatat gcgcataacc atgtatgtaa ataccatgtt taccgtgcta gtgaaatcta
    16201 cgtatggcgt ggacagacgc cattcgtgat gtcgatagct gccacaaggc aacggtcttc
    16261 tcaccgtaga cccaggcatt gcgcgccgtg aatcttcatg atttcggtct atcgtgacgg
    16321 gtagcgactc tgaacagtga tgtttcaggg tcagacagga gtagggaagg aatacagaga
    16381 gacaataata atggtagata gcaagaagcg ccctggcaaa gatctcgacc gtatcgatcg
    16441 taacattctt aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc tttctaaacg
    16501 tgtgggactt tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa gacaagggtt
    16561 tattcagggc tatacggcgc tgcttaaccc ccattatctg gatgcatcac ttctggtatt
    16621 cgttgagatt actctgaatc gtggcgcacc ggatgtgttt gaacaattca ataccgctgt
    16681 acaaaaactt gaagaaattc aggagtgtca tttagtatct ggtgatttcg actacctgtt
    16741 gaaaacacgc gtgccggata tgtcagctta ccgtaagttg ctgggggaaa ccctgctgcg
    16801 tctgcctggc gtcaatgaca cacggacata cgttgttatg gaagaagtca agcagagtaa
    16861 tcgtctggtt attaagacgc gctaacacgg aacaggtgca aaatcggcgt attttgatta
    16921 cactcctgtt aatccataca gcaacagtac tggggtaacc tggtactgtt gtccgtttta
    16981 gcatcgggca ggaaaagcct gtaacctgga gagcctttct tgagccagga atacactgaa
    17041 gacaaagaag tcacattgac aaagttaagt agcggccgcc gccttctgga ggcattgctg
    17101 atccttattg tcctgtttgc cgtctggttg atggctgcct tactaagctt taacccttcg
    17161 gaccccagct ggtcgcaaac ggcctggcat gaacctatcc ataatttagg tgggatgccc
    17221 ggtgcgtggt tggcagatac gctgttcttt atttttggcg tgatggctta caccattccc
    17281 gtcattattg tcggcggttg ttggtttgcc tggcgtcatc agtccagcga cgaatacatt
    17341 gattattttg ccgtttcgct acgcatcatt ggcgttttgg cgctcatcct tacctcctgt
    17401 ggtctggcgg caatcaacgc tgacgatatc tggtattttg cctccggtgg cgtcattggc
    17461 agcttactaa gcactacgct acaaccactg ctacacagta gcgggggaac tattgcgctg
    17521 ctctgcgttt gggcagcggg cctgacgttg ttcaccggtt ggtcatgggt gaccattgct
    17581 gaaaaactcg gcggctggat tttaaacatt ctcaccttcg ccagtaatcg tacccgtcgc
    17641 gatgatacct gggtcgatga agatgagtat gaagacgacg aagagtatga agatgaaaat
    17701 cacggcaaac agcatgaatc acgccgtgcc cgtattcttc gcggcgcgct agcgcgtcgt
    17761 aaacggttgg cggaaaaatt cattaatccg atggggcggc aaacagacgc tgcgttgttc
    17821 tccggtaagc ggatggatga tgacgaagag attacctaca ctgcacgcgg tgtggctgcc
    17881 gacccggacg acgtcctatt ttcgggcaat cgtgcaacgc agccagaata tgacgaatac
    17941 gatccattat taaacagtgc gccaattacc gaacctgtcg ctgtagcagc tgctgctacc
    18001 acggcgacac aaagctgggc tgcgccggtt gaacctgtga ctcagacgcc gcctgttgcc
    18061 tctgttgatg ttccaccttc gcaacctaca gtagcctggc agcctgtacc gggtccacaa
    18121 acgggagagc cggttattgc tcctgcaccg gaaggttacc cacagcagtc acaatatgcg
    18181 cagcctgcag tgcaatataa tgagccgctg caacaaccag tacagccgca gcagccgtat
    18241 tatgcacctg cagctgaaca acctgcgcaa cagccgtatt atgcccctgc gccagaacaa
    18301 ccggtggcag gtaacgcctg gcaagccgaa gagcagcaat ccacttttgc tccacagtct
    18361 acataccaga ctgagcaaac ttatcagcag ccagccgctc aggagccgtt gtaccaacag
    18421 ccgcaacccg ttgaacagca gcctgttgtg gagcctgaac ccgttgtaga agagacaaaa
    18481 cccgcgcgtc cgccgcttta ctattttgaa gaagtggaag agaagcgagc ccgtgaacgt
    18541 gaacaacttg cggcctggta tcaaccgatt ccagaaccgg ttaaagaacc agaaccgatc
    18601 aaatcttcgc tgaaagcacc ttctgttgca gcagtacctc cagtagaagc cgctgccgct
    18661 gtttctccgc tggcatctgg cgtgaaaaaa gcgacactgg cgacgggggc tgccgcaacc
    18721 gttgccgcgc cagtcttcag tctggcaaat agcggtggac cgcgtccgca ggtcaaagag
    18781 gggattggcc cgcagttgcc acgaccgaaa cgtatccgcg tgccaactcg tcgtgaattg
    18841 gcgtcttacg gtattaagct gccctcacag cgtgcggcgg aagaaaaagc ccgtgaagcc
    18901 cagcgcaatc agtatgattc tggcgatcag tataacgatg atgaaatcga tgcgatgcag
    18961 caggatgaac tggcacgtca gttcgcccag acacagcagc aacgctatgg cgaacagtat
    19021 caacatgatg tgcccgtaaa cgcagaagat gcagatgctg cggcagaggc tgaactggct
    19081 cgtcagtttg cgcaaactca acaacaacgt tattccggcg aacaaccggc tggggcgaat
    19141 ccgttctcgc tggatgattt tgaattttcg ccaatgaaag cgttgctgga tgatggtcca
    19201 cacgaaccgc tgtttacgcc aattgttgaa cctgtacagc agccgcaaca accggttgca
    19261 ccacagcagc agtatcagca gccacaacag ccggttgcgc cacagcagca gtatcagcag
    19321 ccacaacagc aggttgcgcc gcagccacaa tatcagcagc cgcaacaacc ggttgcgcca
    19381 cagcagcaat atcagcagcc gcaacaacca gttgcgccgc agccgcagta tcaacagccg
    19441 caacaaccgg ttgcaccgca gccgcagtat cagcagccac aacagccagt tgcgccacag
    19501 ccgcaggata ccctgcttca tccgctgttg atgcgtaatg gcgacagccg tccgttgcac
    19561 aaaccgacga cgccactgcc ttctctggat ttgctgacac cgccgccgag cgaagtggag
    19621 ccggtagata cctttgcgct tgaacaaatg gcgcgtctgg tggaagcgcg tctggctgat
    19681 ttccgtatta aagccgatgt cgttaattac tctccggggc cagttatcac tcgctttgaa
    19741 cttaacctgg ctcctggcgt aaaagcggcg cgcatttcta acttgtcacg ggaccttgcc
    19801 cgttcacttt cgacggtggc ggtgcgtgtc gttgaagtta ttcctggcaa accctatgta
    19861 ggtctggagt taccaaataa aaaacgacaa accgtttatc tgcgcgaagt tttggataac
    19921 gccaaattcc gcgataatcc gtcgccatta accgtggtgc tgggtaaaga tatcgccggt
    19981 gagccggtgg ttgccgatct ggcgaaaatg ccgcacttgt tggttgcggg gactaccggt
    20041 tccggtaaat ctgtcggtgt gaacgcgatg atcctgagca tgctttataa agcacagcca
    20101 gaagatgtgc gtttcatcat gatcgacccg aaaatgctgg agctttcggt ttatgaaggc
    20161 attccgcacc tgttaacgga agtcgttact gatatgaaag atgccgccaa cgcgctgcgc
    20221 tggtgtgtta acgagatgga gcgtcggtat aaactgatgt ctgcgctggg tgtgcgtaat
    20281 ctggcgggtt ataacgaaaa aattgctgaa gccgatcgca tgatgcgtcc gattccagac
    20341 ccgtactgga agccgggtga cagtatggat gcccagcatc cggtgctgaa aaaagaacca
    20401 tacattgtcg tgttggttga cgaatttgcc gacctgatga tgacggtagg taaaaaagtg
    20461 gaagagctga tagctcgtct ggcgcaaaaa gcccgtgccg cgggtatcca cctcgtactg
    20521 gcaactcagc gtccatcggt tgatgttatt actggtctga ttaaagcgaa tattccgacc
    20581 cgtatcgcct ttaccgtatc cagtaagatt gactcacgta ccattcttga tcaggctggc
    20641 gcggaatcac tgctgggtat gggggatatg ctctactctg ggccgaactc cacgttgccg
    20701 gtacgtgtcc atggtgcttt tgttcgcgat caggaagttc atgccgtggt gcaggactgg
    20761 aaagcgcgtg gtcgcccaca gtatgttgat ggcatcacct ccgacagcga aagcgaaggt
    20821 ggtgcgggtg gtttcgatgg cgctgaagaa ctggatccgt tgttcgatca ggcggtgcag
    20881 tttgtcactg aaaaacgcaa agcgtcaatt tctggcgtac agcgtcagtt ccgcattggt
    20941 tataaccgtg cagcgcgtat tatcgaacag atggaagcgc aggggattgt cagcgaacag
    21001 gggcacaacg gtaatcgtga agtgctggcc ccaccgccgt ttgactaatt aatgcattgc
    21061 cggataaggc gcggtagcgt cgcatccggc actctatcaa ctgaaaattc agtattttct
    21121 tctttcctca agctgattat tagcctggaa tagagagtag agggaactcc cgatcgggag
    21181 tgacgtaatt tgaggaataa tgatgaaaaa aattgctatc acctgtgcat tactctcaag
    21241 cttagtagca agcagcgttt gggctgatgc cgcaagcgat ctgaaaagcc gcctggataa
    21301 agtcagcagc ttccacgcca gcttcacaca aaaagtgact gacggtagcg gcgcggcggt
    21361 gcaggaaggt cagggcgatc tgtgggtgaa acgtccaaac ttattcaact ggcatatgac
    21421 acaacctgat gaaagcattc tggtttctga cggtaaaaca ctgtggttct ataacccgtt
    21481 cgttgagcaa gctacggcaa cctggctgaa agatgccacc ggtaatacgc cgtttatgct
    21541 gattgcccgc aaccagtcca gcgactggca gcagtacaat atcaaacaga atggcgatga
    21601 ctttgtcctg acgccgaaag ccagcaatgg caatctgaag cagttcacca ttaacgtggg
    21661 acgtgatggc acaatccatc agtttagcgc ggtggagcag gacgatcagc gcagcagtta
    21721 tcagctgaaa tcccagcaaa atggggctgt ggatgcagcg aaatttacct tcaccccgcc
    21781 gcaaggcgtc acggtagatg atcaacgtaa gtagaggcac ctgagtgagc aatctgtcgc
    21841 tcgatttttc ggataatact tttcaacctc tggccgcgcg tatgcggcca gaaaatttag
    21901 cacagtatat cggtcagcaa catttgctgg ctgcggggaa gccgttgccg cgcgctatcg
    21961 aagccgggca tttgcattct atgatcctct gggggccacc gggtaccggc aaaacaaccc
    22021 tcgctgaagt gattgcccgc tatgcgaacg ctgatgtgga acgtatttct gccgtcacct
    22081 ctggcgtgaa agagattcgc gaggcgatcg agcgcgcccg gcaaaaccgc aatgcaggtc
    22141 gccgcactat tctttttgtt gacgaagttc accgtttcaa caaaagccag caggatgcat
    22201 ttctgccaca tattgaagac ggcaccatca cttttattgg cgcaaccact gaaaacccgt
    22261 cgtttgagct taattcggca ctgctttccc gtgcccgtgt ttatctgttg aaatccctga
    22321 gtacagagga tattgagcaa gtactaactc aggcgatgga agacaaaacc cgtggctatg
    22381 gtggtcagga tattgttctg ccagatgata cacgacgtgc cattgcagaa ctggtgaatg
    22441 gcgacgcgcg ccgggcgtta aatacgctgg aaatgatggc ggatatggcc gaagtctatg
    22501 atagcggtaa gcgggtcctg aagcctgaat tactgaccga aatcgccggt gaacgtagcg
    22561 cccgctttga taacaaaggc gatcgctttt acgatctaat ttccgcactg cataagtcgg
    22621 tacgtggtag cgcacccgat gcggcgctgt actggtatgc gcgaattatt accgctggtg
    22681 gcgatccgtt atatgtcgcg cgtcgctgtc tggcgattgc gtctgaagac gtcggtaatg
    22741 ccgatccacg ggcgatgcag gtggcaattg cggcctggga ttgctttact cgcgttggcc
    22801 cggcggaagg tgaacgcgcc attgctcagg cgattgttta cctggcctgc gcgccaaaaa
    22861 gcaacgctgt ctacactgcg tttaaagccg cgctggccga tgctcgcgaa cgcccggatt
    22921 atgacgtgcc ggttcatttg cgtaatgcgc cgacgaaatt aatgaaggaa atgggctacg
    22981 ggcaggaata tcgttatgct catgatgaag caaacgctta tgctgccggt gaggtttact
    23041 tcccgccgga aatagcacaa acacgctatt atttcccgac aaacaggggc cttgaaggca
    23101 agattggtga aaagctcgcc tggctggctg aacaggatca aaatagcccc ataaaacgct
    23161 accgttaatg ttatcgttgc ggtaatgttg ttactgtatc cctgtggtcg caggctgtgg
    23221 ccacatctcc catttaattc gataagcaca ggataagcat gctcgatccc aatctgctgc
    23281 gtaatgagcc agacgcagtc gctgaaaaac tggcacgccg gggctttaag ctggatgtag
    23341 ataagctggg cgctcttgaa gagcgtcgta aagtattgca ggtcaaaacg gaaaacctgc
    23401 aagcggagcg taactcccga tcgaaatcca ttggccaggc gaaagcgcgc ggggaagata
    23461 tcgagccttt acgtctggaa gtgaacaaac tgggcgaaga gctggatgca gcaaaagccg
    23521 agctggatgc tttacaggct gaaattcgcg atatcgcgct gaccatccct aacctgcctg
    23581 cagatgaagt gccggtaggt aaagacgaaa atgacaacgt tgaagtcagc cgctggggta
    23641 ccccgcgtga gtttgacttt gaagttcgtg accacgtgac gctgggtgaa atgcactctg
    23701 gcctcgactt tgcagctgca gttaagctga ctggttcccg ctttgtggta atgaaagggc
    23761 agattgctcg catgcaccgc gcactgtcgc agtttatgct ggatctgcat accgaacagc
    23821 atggctacag tgagaactat gttccgtacc tggttaacca ggacacgctg tacggtacgg
    23881 gtcagctgcc gaaatttgct ggcgatctgt tccatactcg tccgctggaa gaagaagcag
    23941 acaccagtaa ctatgcgctg atcccaacgg cagaagttcc gctgactaac ctggtgcgcg
    24001 gtgaaatcat cgatgaagat gatctgccaa ttaagatgac cgcccacacc ccatgcttcc
    24061 gttctgaagc cggttcatat ggtcgtgaca cccgtggtct gatccgtatg caccagttcg
    24121 acaaagttga aatggtgcag atcgtgcgcc cagaagactc aatggcggcg ctggaagaga
    24181 tgactggtca tgcagaaaaa gtcctgcagt tgctgggcct gccgtaccgt aaaatcatcc
    24241 tttgcactgg cgacatgggc tttggcgctt gcaaaactta cgacctggaa gtatggatcc
    24301 cggcacagaa cacctaccgt gagatctctt cctgctccaa cgtttgggat ttccaggcac
    24361 gtcgtatgca ggcacgttgc cgcagcaagt cggacaagaa aacccgtctg gttcataccc
    24421 tgaacggttc tggtctggct gttggtcgta cgctggttgc agtaatggaa aactatcagc
    24481 aggctgatgg tcgtattgaa gtaccagaag ttctgcgtcc gtatatgaac ggactggaat
    24541 atattggcta atacccaatt tttctgaatc taaaaagcgc ctttagggcg ctttttttgt
    24601 ctccctttga taccgaacaa taattactcc tcacttacac gtaatactac tttcgagtga
    24661 aaatctacct atctctttga ttttcaaatt attctatgta tacaagccta tatagcgagc
    24721 tgctatagaa ataattacac aatacggttt gttactggaa tcaatcgtga tcaagcttga
    24781 gtgagccatt atgaaaacga aaatccctga tgcggtattg gctgctgagg agagtcgccg
    24841 tggtttggta aaaacgacag cgatcggcgg cctggcaatg gccagcagcg cattaacatt
    24901 accttttagt cggattgcgc acgctgtcga tagcgccatt ccaacaaaat cagacgaaaa
    24961 ggttatctgg agcgcctgta cagttaactg tggtagtcgc tgcccgctac gtatgcacgt
    25021 cgtggacggt gaaatcaaat atgtcgaaac gacaataccg gcgatgacaa ttacgacggc
    25081 ctacactagg ttcgcgcctg cctgcgtggg cgttccatgc gtcaccgtgt ctacaatccg
    25141 gaccgcctga aatatccgat gaaacgagtc ggggcgcgcg gtgaaggcaa attcgagcgc
    25201 attagctggg aagaagccta cgacatcatc gcgaccaata tgcagcgcct gatcaaagag
    25261 tacggcaacg agtctatcta tctgaactat ggcaccggta cgctgggcgg caccatgacc
    25321 cgctcctggc cgccgggaaa taccctggtc gcgcgcctga tgaactgctg cggcggttat
    25381 cttaaccatt acggtgacta ctcttccgcg caaattgcgg aaggtttgaa ccatacctac
    25441 ggcggctggg cagatggtaa cagcccgtcg gatatcgaaa acagtaaatt ggtagtgctg
    25501 tttggtaata acccaggcga aacgcgaatg agtggcggtg gggtgactta ctatcttgaa
    25561 caggcacgcc agaaatctaa tgctcgcatg atcatcatcg atccgcgcta taccgacacc
    25621 ggtgccgggc gcgaagatga gtggatccct attcgtccag gaacagatgc cgcactggtt
    25681 aacggtctgg cgtacgtcat gatcactaaa aacctggtgg atcaggcatt cctcgataaa
    25741 tattgcgttg gctatgacga gaaaaccctg cccgccagtg cgccgaaaaa tggtcattat
    25801 aaagcttata ttctgggtga agggccagat ggcgtggcta aaacaccgga atgggcctcg
    25861 caaatcaccg gtgttccggc agacaaaatc atcaaactgg ctcgtgaaat cggcagtacc
    25921 aaaccggcgt ttatcagcca gggatggggc ccgcagcgtc acgctaacgg tgaaatcgca
    25981 acccgtgcta tctcgatgct ggcgattctg accggtaacg ttggtattaa tggagggaac
    26041 agcggcgcgc gtgaaggttc atacagctta ccgtttgtcc gtatgccgac cttggaaaac
    26101 ccgatccaga ccagcatttc gatgtttatg tggaccgatg ccattgaacg tggcccggaa
    26161 atgacggcgc tgcgtgatgg tgtgcgcggg aaagataagc tggatgtgcc gatcaaaatg
    26221 atctggaact atgccggtaa ctgcctgatt aaccagcatt ctgaaatcaa ccgtacccat
    26281 gaaatcctcc aggatgataa gaagtgcgag ctgattgtgg ttatcgactg ccacatgacc
    26341 tcatcggcga aatatgctga catcctgctg cctgactgca ccgcttccga acagatggac
    26401 tttgcactgg atgcatcctg cgggaatatg tcttacgtga ttttcaacga tcaggtgatt
    26461 aaaccgcgct ttgaatgtaa gaccatctat gaaatgacca gcgaactggc aaaacgtctt
    26521 ggcgttgagc aacagtttac tgaaggccgt acccaggaag agtggatgcg gcatctgtat
    26581 gcccagtcgc gggaagcgat tcctgaactg ccaacgtttg aagagttccg caagcagggg
    26641 atctttaaaa agcgcgaccc acaagggcat cacgttgctt ataaagcctt ccgtgaagat
    26701 ccgcaggcaa acccactgac tacgccatcg ggcaaaattg agatttattc gcaggcgctg
    26761 gctgacattg ccgctacctg ggaattgcca gaaggcgatg tgatcgatcc actgccgatc
    26821 tacacgccgg gctttgaaag ttatcaggat ccgctgaaca aacagtatcc gctgcagctt
    26881 acaggtttcc actataaatc tcgcgttcac tcaacttacg gcaacgttga tgtgctgaaa
    26941 gcggcttgcc gtcaggaaat gtggatcaac ccgcttgatg cccaaaaacg cggtatccac
    27001 aacggcgata aagtcaggat ctttaacgat cgtggtgagg ttcatattga ggcgaaagtg
    27061 acgccacgaa tgatgccggg cgtggtcgca ctgggtgaag gcgcctggta tgacccggat
    27121 gcaaaacgtg tcgataaggg cggttgtatc aacgtactga ccactcaacg tccgtctcct
    27181 ctcgctaagg ggaatccgtc acatacaaac cttgttcagg ttgaaaaggt gtaaggagta
    27241 accgatgaca acccagtatg gattttttat tgattccagc cgttgcaccg gttgcaaaac
    27301 ctgcgagctg gcttgtaaag actacaaaga tttgacgcca gaagtcagtt tccgccgcat
    27361 ttatgaatat gctggcggcg actggcagga agataacggt gtctggcacc agaacgtgtt
    27421 tgcctactat ctgtcgattt cgtgcaacca ctgcgaagat ccggcttgta ctaaagtctg
    27481 cccgagcggt gcgatgcata aacgcgaaga tggttttgtt gtggtcgatg aagatgtatg
    27541 cattggctgc cgttactgtc atatggcttg cccgtatggc gcaccgcaat ataacgaaac
    27601 gaaaggccat atgaccaaat gcgatggttg ttatgaccgt gttgcggagg gtaaaaagcc
    27661 gatctgtgtt gaatcttgcc cgctgcgggc gctggatttc ggccctatcg acgagctgcg
    27721 taaaaaacat ggcgatctgg cggccgttgc gccgttgccg cgagctcact ttaccaaacc
    27781 gaatattgtg atcaaaccca atgccaatag ccgcccgacc ggggatacca ctggctatct
    27841 ggcaaacccg aaggaggtgt gagatgggaa gtggatggca tgaatggccg ctgatgatct
    27901 tcacggtctt cgggcaatgt gtggcgggtg gttttatcgt tctggctttg gcgctgctca
    27961 aaggcgacct gcgagcagaa gcccagcagc gtgttatcgc ctgcatgttt ggcttatggg
    28021 tactgatggg cattggcttt atcgcctcta tgctccatct tggttcaccg atgcgcgcct
    28081 ttaactcgct caaccgggta ggggcttcag cactcagtaa cgaaatcgcc agcggttcga
    28141 tcttttttgc tgtaggcggc atcggctggt tgctggcaat gctgaaaaag ctgtcaccgg
    28201 cattgcgtac gctatggctg gtggtgacga tggttcttgg cgtcgtcttt gtctggatga
    28261 tggtgcgtgt gtataacagc attgataccg ttccgacctg gtacagcatc tggacgccga
    28321 tgggctgggc gatgcgtctg ctgccagcca tttctgtact ggcactggtg gtgagtggcg
    28381 tgatgtcagt gatgcagggc acagagctgg caactattca tagttctgtg cagcaggccg
    28441 cagcgctggt gccggactat ggtgcgctga tgtcctggcg gatcgtgctt ttggccgttg
    28501 ccctgtgctt gtggattgca ccacagctaa aaggttatca gcctgcggtt ccgctactat
    28561 ccgtctcatt cattctgctt ctggcagggg aattaatcgg tcgcggcgta ttctatggtt
    28621 tgcatatgac cgtggggatg gccgtcgcaa gctaatcagt aacaaccggg gtttcggccc
    28681 cggttctctt ttatttctgc ttcgttaacg tgtcataact ggtcatcaaa ttacgataat
    28741 caggaatatg gttagagaac aacgtcgcca gtccttcaat atcattacgc cagtcacggt
    28801 gcagctcgca agccacgccg aaccatgtca tcaattgcgc accagcttgc gacatgcgat
    28861 cccacgccga atgccgggta atttcattaa aggtccctga tgcatcagtc acgacaaaga
    28921 catcaaaccc ttcttcaatg gctgaaagcg ccgggaatgc cacgcaaact tcggttacca
    28981 caccggcaat aattaactgt tttttacctg tcgctttgac tgcttttaca aaatcttcgt
    29041 tatcccaggc gttaatattg cccgggcgag caatgtaagg tgcatcgggg aattgtgctt
    29101 tcagttctag aactaatggg ccgttaggac cggtttcaaa actggtggta agaatagttg
    29161 gtaagttgaa atacttagcc aggtcaccca gcgccagcac gttattttta aacttatcgg
    29221 gttcgatatc ccgtacaagg gaaagtaaac cagcctggtg atcaacaagc aaaacggcag
    29281 catcattttt atcaagacga acatacggtt tggtcatcct cttctccttt cgagagtcag
    29341 tggtacgtct gaaatcggtc agacgcgatg cattgctctg aaagcataga cgggaaatat
    29401 gagtttgctg tgaccatgaa atttttcgac tgaacgaagc gtatgaaatt tgtgttagat
    29461 caataaaaac aatcagatga gattaatcat ctcttcagca ttagcgttat gaatgagtaa
    29521 tataaatcga taataccgcc gctgcgatat gcgcgtattt tcacccattg acaatgtttt
    29581 tggcggtggc atgatgcgca tgaaatttga acttcctcac ggttttaatt catgtccacg
    29641 tatacccggc ctgtcatgct tttgctgtct ggcctgcttt tgttgactct ggcgatcgcg
    29701 gtgttaaata cactcgtgcc gctttggctc gcccaggaac acatgtccac atggcaggta
    29761 ggcgttgtca gctcatccta ttttaccggc aaccttgtcg gtacattgct gacagggtat
    29821 gtcattaaac gcattggctt taaccgcagc tattatctgt cctccttcat ttttgctgct
    29881 ggctgtgccg gccttggcct gatgattggt ttctggagct ggttggcgtg gcgttttgtc
    29941 gcgggcgtcg gctgtgccat gatttgggtg gttgttgaga gcgcgctgat gtgcagtggg
    30001 acgtcacgta accgtgggcg tttgcttgct gcgtatatga tggtttatta cgtgggaacg
    30061 tttttaggcc agttactggt cagcaaagtc tcaaccgagc tgatgagcgt attgccgtgg
    30121 gttacaggtt tgacattggc agggatctta ccgctgttgt ttacgcgtgt cctgaatcag
    30181 caggctgaaa accatgattc gacgtcaatt acggcaatgc taaaactccg tcaggcgcgg
    30241 cttggcgtga atggctgcat tatctcagga atcgttctgg gatctctata tggcctgatg
    30301 cctctgtacc tcaatcacaa aggggtgagc aatgccagta ttggtttctg gatggcggta
    30361 ctggtcagtg cgggtatcct cggacaatgg ccgattggac gtctggcgga taagtttggt
    30421 cgactgctgg ttttgcgtgt tcaggtcttt gtcgtcattc tcggcagtat tgcgatgctt
    30481 agccaggcgg cgatggcccc agcgttattc atcctcggtg ccgctggctt tacgctatat
    30541 ccggtggcga tggcctgggc ttgtgagaaa gttgaacatc atcaactggt ggcgatgaac
    30601 caagccttac tgttgagcta taccgtggga agtctgcttg gcccgtcatt tagcgctatg
    30661 ctaatgcaga atttctccga taatttattg tttatcatga tcgccagcgt atcgtttatc
    30721 tatttgctga tgctgcttcg caacgccggt catacgccga aacccgttgc tcacgtgtaa
    30781 atgaattcaa gcaaagtgtg aacttactgt ttcacactct gcttttttgt ttcttctatc
    30841 tgacttgctt ttttccaaat tttattcgtt taaaaataaa atgtgcagca ggttataatt
    30901 ttgcatttcg ctatttccgc acttcttatt tgccgcgcat aatccctcgt tttacagatg
    30961 cccctttaat tttggcgaag gatttgtcta tggctgg
//
LOCUS       DAJTUI010000041        30117 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTUI010000041 DAJTUI010000000
VERSION     DAJTUI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30117
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.041"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            257..640
                     /gene="secE"
                     /locus_tag="OO464_002027"
     CDS             257..640
                     /gene="secE"
                     /locus_tag="OO464_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6227182.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            642..1187
                     /gene="nusG"
                     /locus_tag="OO464_002028"
     CDS             642..1187
                     /gene="nusG"
                     /locus_tag="OO464_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6227183.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1346..1774
                     /gene="rplK"
                     /locus_tag="OO464_002029"
     CDS             1346..1774
                     /gene="rplK"
                     /locus_tag="OO464_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6227184.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1778..2482
                     /gene="rplA"
                     /locus_tag="OO464_002030"
     CDS             1778..2482
                     /gene="rplA"
                     /locus_tag="OO464_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6227185.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2895..3392
                     /gene="rplJ"
                     /locus_tag="OO464_002031"
     CDS             2895..3392
                     /gene="rplJ"
                     /locus_tag="OO464_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6227186.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3459..3824
                     /gene="rplL"
                     /locus_tag="OO464_002032"
     CDS             3459..3824
                     /gene="rplL"
                     /locus_tag="OO464_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6227187.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4144..8172
                     /gene="rpoB"
                     /locus_tag="OO464_002033"
     CDS             4144..8172
                     /gene="rpoB"
                     /locus_tag="OO464_002033"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6227188.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8249..12472
                     /gene="rpoC"
                     /locus_tag="OO464_002034"
     CDS             8249..12472
                     /gene="rpoC"
                     /locus_tag="OO464_002034"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6227189.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12714..13847)
                     /gene="thiH"
                     /locus_tag="OO464_002035"
     CDS             complement(12714..13847)
                     /gene="thiH"
                     /locus_tag="OO464_002035"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6227190.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13844..14614)
                     /gene="thiG"
                     /locus_tag="OO464_002036"
     CDS             complement(13844..14614)
                     /gene="thiG"
                     /locus_tag="OO464_002036"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6227191.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14616..14816)
                     /gene="thiS"
                     /locus_tag="OO464_002037"
     CDS             complement(14616..14816)
                     /gene="thiS"
                     /locus_tag="OO464_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6227192.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14800..15555)
                     /gene="thiF"
                     /locus_tag="OO464_002038"
     CDS             complement(14800..15555)
                     /gene="thiF"
                     /locus_tag="OO464_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR6227193.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15548..16183)
                     /gene="thiE"
                     /locus_tag="OO464_002039"
     CDS             complement(15548..16183)
                     /gene="thiE"
                     /locus_tag="OO464_002039"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6227194.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16183..18078)
                     /gene="thiC"
                     /locus_tag="OO464_002040"
     CDS             complement(16183..18078)
                     /gene="thiC"
                     /locus_tag="OO464_002040"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6227195.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18159..18259)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18312..18788)
                     /gene="rsd"
                     /locus_tag="OO464_002041"
     CDS             complement(18312..18788)
                     /gene="rsd"
                     /locus_tag="OO464_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6227196.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18883..19656
                     /gene="nudC"
                     /locus_tag="OO464_002042"
     CDS             18883..19656
                     /gene="nudC"
                     /locus_tag="OO464_002042"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6227197.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19696..20760
                     /gene="hemE"
                     /locus_tag="OO464_002043"
     CDS             19696..20760
                     /gene="hemE"
                     /locus_tag="OO464_002043"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6227198.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20917..21588
                     /gene="nfi"
                     /locus_tag="OO464_002044"
     CDS             20917..21588
                     /gene="nfi"
                     /locus_tag="OO464_002044"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6227199.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21631..22221
                     /locus_tag="OO464_002045"
     CDS             21631..22221
                     /locus_tag="OO464_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6227200.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22408..22680
                     /gene="hupA"
                     /locus_tag="OO464_002046"
     CDS             22408..22680
                     /gene="hupA"
                     /locus_tag="OO464_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR6227201.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22693..23388
                     /locus_tag="OO464_002047"
     CDS             22693..23388
                     /locus_tag="OO464_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6227202.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23390..23815)
                     /gene="zraP"
                     /locus_tag="OO464_002048"
     CDS             complement(23390..23815)
                     /gene="zraP"
                     /locus_tag="OO464_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6227203.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24053..25429
                     /gene="zraS"
                     /locus_tag="OO464_002049"
     CDS             24053..25429
                     /gene="zraS"
                     /locus_tag="OO464_002049"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6227204.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25426..26751
                     /gene="zraR"
                     /locus_tag="OO464_002050"
     CDS             25426..26751
                     /gene="zraR"
                     /locus_tag="OO464_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR6227205.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26748..28037)
                     /gene="purD"
                     /locus_tag="OO464_002051"
     CDS             complement(26748..28037)
                     /gene="purD"
                     /locus_tag="OO464_002051"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6227206.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28049..29638)
                     /gene="purH"
                     /locus_tag="OO464_002052"
     CDS             complement(28049..29638)
                     /gene="purH"
                     /locus_tag="OO464_002052"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6227207.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7058 a         7848 c         8282 g         6929 t
ORIGIN      
        1 ggcgttgtag caaaagttct gagctaattg ccgataacat ttgacgcaat gcgcactaaa
       61 agggcatcat ttgatgccct ttttgcacgc tttcgtacca gaacctggct catcagtgat
      121 tttctttgtc ataatcattg ctgagacagg ctctgttgag ggcgtataat ccgaaaagct
      181 aatacgcgtt tcgatttggt ttgcctcgcg atcgcggggt gaaaatgttt gtagaaaact
      241 tctgacaggt tggtttatga gtgcgaatac cgaagctcaa ggaagcgggc gcggcctgga
      301 agcgatgaag tgggtcgttg tggtggcatt gctcctggcg gcgattgtcg gcaactacct
      361 ttatcgcgac attatgctgc cgctgcgtgc gctggccgta gtaattctga ttgctgcagc
      421 gggtggtgtc gcgctgttaa cgacaaaagg taaagctacc attgcttttg cccgtgaagc
      481 gcgtaccgaa gtccgtaagg tcatttggcc gactcgccag gaaacattgc acaccacgct
      541 gattgtggct gcggttaccg cagtaatgtc actgatcctg tggggactgg atggtattct
      601 ggttcgcctg gtatccttta tcactggcct gaggttctga gatgtctgaa gctcctaaaa
      661 agcgctggta cgtcgttcag gcgttttccg gttttgaagg ccgcgtagca acgtcgctgc
      721 gtgagcatat caaattacac aacatggaag atttgtttgg tgaagtcatg gtaccaaccg
      781 aagaagtggt tgaaatccgt ggcggtcagc gtcgcaaaag cgaacgtaaa ttcttccctg
      841 gctacgtcct cgttcagatg gtgatgaacg acgcgagctg gcacttggtg cgcagcgtac
      901 cgcgtgtgat gggcttcatc ggcggtactt ccgatcgtcc tgcgccaatc agcgataaag
      961 aagtcgatgc gattatgaac cgcctgcagc aggttggtga taagccgcgt ccgaaaacgc
     1021 tgtttgaacc gggtgaaatg gtccgtgtta atgatggtcc gttcgctgac ttcaacggtg
     1081 ttgttgaaga agtggattac gagaaatctc gtctgaaagt gtctgtttct atcttcggtc
     1141 gtgcgacccc ggtagagctg gacttcagcc aggttgaaaa agcctaaccc agcgatcaaa
     1201 aaagcggcga tttaatcgtt gcacaaggcg tgagattgga atacaatttc gcgccttttg
     1261 tttttatggg ccttgcccgt aaaacgattt tttatatcac ggggagcctc tcagaggcgt
     1321 tattacccaa cttgaggaat ttataatggc taagaaagta caagcctatg tcaagctgca
     1381 ggttgcagct ggtatggcta acccgagtcc gccagtaggt ccggctctgg gtcagcaggg
     1441 cgtaaacatc atggaattct gcaaagcgtt caacgcaaaa actgattcca tcgaaaaagg
     1501 tctgccgatt ccggtagtaa tcaccgttta cgctgaccgt tctttcactt tcgttaccaa
     1561 gaccccgccg gcagcagttc tgctgaaaaa agcggcaggt atcaagtctg gttccggtaa
     1621 gccgaacaaa gacaaagtgg gtaaaatttc ccgcgctcag ctgcaggaaa tcgcgcagac
     1681 caaagctgcc gacatgactg gtgccgacat tgaagcgatg actcgctcca tcgaaggtac
     1741 tgcacgttcc atgggcctgg tagtggagga ctaagaaatg gctaaactga ccaagcgcat
     1801 gcgtgttatc cgtgagaaag ttgatgcaac caaacagtac gacatcaacg aagctatcgc
     1861 actgctgaaa gagctggcga ctgctaaatt cgtagaaagc gtggacgtag ctgttaacct
     1921 cggcatcgac gctcgtaaat ctgaccagaa cgtacgtggt gcaactgtac tgccgcacgg
     1981 tactggccgt tccgttcgcg tagccgtatt tacccaaggt gcaaacgctg aagctgctaa
     2041 agctgcaggc gcagaactgg taggtatgga agatctggct gaccagatca agaaaggcga
     2101 aatgaacttt gacgttgtta ttgcttctcc ggatgcaatg cgcgttgttg gccagctggg
     2161 ccaggttctg ggtccgcgtg gcctgatgcc aaacccgaaa gtgggtactg taacaccgaa
     2221 cgttgctgaa gcggttaaaa acgctaaagc tggccaggtt cgttaccgta acgacaaaaa
     2281 cggcatcatc cacaccacca tcggtaaagt ggactttgac gctgacaaac tgaaagaaaa
     2341 cctggaagct ctgctggttg cgctgaaaaa agcaaaaccg actcaggcga aaggcgtgta
     2401 catcaagaaa gttagcatct ccaccaccat gggtgcaggt gttgcagttg accaggctgg
     2461 cctgagcgct tctgtaaact aatgccttta cgtgggcggt gattttgtct acaatcttac
     2521 ccccacgtat aatgcttaat gcagacgtat atccgagata ttcgggttgt ggcaaggcgg
     2581 caactgagtg agtcgccaag agcatagcta actatgtgac tggtgcgaat gaaggaagcc
     2641 aacgccgcca caagttgaat agcgacggat agaaaagatt tgttcgttgg agcctggcct
     2701 atccaggcct ccgtcgaaga ccgcaggagt ttcgcaagaa acttaatccc ctgcgtagac
     2761 ggtgacagaa cgctaagatt attcttttat attctggctt gtttctgctc accgtaatta
     2821 agacgctctc tccgtttgga ggagtgaagt gagttccaga gattttctct ggcaaacatc
     2881 caggagcaaa gctaatggct ttaaatcttc aagacaaaca agcgattgtt gctgaagtca
     2941 gcgaagtagc caaaggcgcg ctgtctgcag tagttgcgga ttcccgtggc gtaactgtag
     3001 ataaaatgac tgaactgcgt aaagcaggtc gcgaagctgg cgtatacatg cgtgttgttc
     3061 gtaacaccct gctgcgccgt gctgttgaag gtactccgtt cgagtgcctg aaagacgcgt
     3121 ttgttggtcc gaccctgatt gcatactcta tggaacaccc gggcgctgct gctcgtctgt
     3181 tcaaagagtt cgcgaaagcg aatgcaaaat ttgaggtcaa agccgctgcc tttgaaggtg
     3241 agctgatccc ggcgtctcag atcgaccgcc tggcaactct gccgacctac gaagaagcaa
     3301 ttgcacgcct gatggcaacc atgaaagaag cttcggctgg caaactggtt cgtactctgg
     3361 ctgctgtacg cgatgcgaaa gaagctgctt aatcgcagtt atctttttaa cgcattcgct
     3421 tacgtataaa cttattctga tattcaggaa caatttaaat gtctatcact aaagatcaaa
     3481 tcattgaagc agttgcagct atgtctgtaa tggacgttgt agaactgatc tctgcaatgg
     3541 aagaaaaatt cggtgtttcc gctgctgctg ctgtagctgt agctgctggc ccggttgaag
     3601 ctgctgaaga aaaaactgaa ttcgacgtaa ttctgaaagc tgctggcgct aacaaagttg
     3661 ctgttatcaa agcagtacgt ggcgcaactg gcctgggtct gaaagaagct aaagacctgg
     3721 tagaatctgc accggctgct ctgaaagaag gcgtgagcaa agacgacgca gaagcactga
     3781 aaaaagctct ggaagaagct ggcgctgaag ttgaagttaa ataagccaac ccttccggtt
     3841 gcagcctgag aaatcaggct gatggctggt gactttttag tcaccagcct ttttgcgctg
     3901 taaggcgcca gtagcgtttc acactgtttg actactgctg tgcctttcaa tgcttgtttc
     3961 tatcgacgac ttaatatact gcgacaggac gtccgttctg tgtaaatcgc aatgaaatgg
     4021 tttaagcgtg atagcaacag gcattgcgga aagtgttcca ttttccggtc aacaaaatag
     4081 tgttgcacaa actgtccgct caatggacag atgggtcgac ttgtcagcga gctgaggaac
     4141 cctatggttt actcctatac cgagaaaaaa cgtattcgta aggattttgg taaacgtcca
     4201 caagttctgg atgtacctta tctcctttct atccagcttg actcgtttca gaaatttatc
     4261 gagcaagatc ctgaagggca gtatggtctg gaagctgctt tccgttccgt attcccgatt
     4321 cagagctaca gcggtaattc cgagctgcaa tacgtcagct accgccttgg cgaaccggtg
     4381 tttgacgtcc aggaatgtca aatccgtggc gtgacctatt ccgcaccgct gcgcgttaaa
     4441 ctgcgtctgg tgatctatga gcgcgaagcg ccggaaggca ccgtaaaaga cattaaagaa
     4501 caagaagtct acatgggcga aattccgctc atgacagaca acggtacctt tgttatcaac
     4561 ggtactgagc gtgttatcgt ttcccagctg caccgtagtc cgggcgtctt ctttgactcc
     4621 gacaaaggta aaacccactc ttcgggtaaa gtgctgtata acgcgcgcat catcccttac
     4681 cgtggttcct ggctggactt cgaattcgat ccgaaggaca acctgttcgt acgtatcgac
     4741 cgtcgccgta aactgcctgc gaccatcatt ctgcgcgccc tgaactacac cacagagcag
     4801 atcctcgacc tgttctttga aaaagttatc tttgaaatcc gtgataacaa gctgcagatg
     4861 gaactggtgc cggaacgcct gcgtggtgaa accgcatctt ttgacatcga agctaacggt
     4921 aaagtgtacg tagaaaaagg ccgccgtatc actgcgcgcc acattcgcca gctggaaaaa
     4981 gacgacgtca aactgattga agtcccggtt gagtacatcg caggtaaagt ggttgctaaa
     5041 gactatattg atgagtctac cggcgagctg atctgcgcag cgaacatgga gctgagcctg
     5101 gatctgctgg ctaagctgag ccagtctggt cacaagcgta tcgaaacgct gttcaccaac
     5161 gatctggatc acggcccata tatctctgaa accttacgtg tcgacccaac taacgaccgt
     5221 ctgagcgcac tggtagaaat ctaccgcatg atgcgccctg gcgagccgcc gactcgtgaa
     5281 gcagctgaaa gcctgttcga gaacctgttc ttctccgaag accgttatga cttgtctgcg
     5341 gttggtcgta tgaagttcaa ccgttctctg ctgcgcgaag aaatcgaagg ttccggtatt
     5401 ctgagcaaag acgacatcat tgatgttatg aaaaagctca tcgatatccg taacggtaaa
     5461 ggcgaagtcg atgatatcga ccacctcggc aaccgtcgta tccgttccgt tggcgaaatg
     5521 gcggaaaacc agttccgcgt tggcctggta cgtgtagagc gtgcggtgaa agagcgtctg
     5581 tctctgggcg atctggatac cctgatgcct caggatatga tcaacgccaa gccgatttcc
     5641 gcagcagtga aagagttctt cggttccagc cagctgtctc agtttatgga ccagaacaac
     5701 ccgctgtctg agattacgca caaacgtcgt atctccgcac tcggcccagg cggtctgacc
     5761 cgtgaacgtg caggcttcga agttcgagac gtacacccga ctcactacgg tcgcgtatgt
     5821 ccaatcgaaa cccctgaagg tccgaacatc ggtctgatca actctctgtc cgtgtacgca
     5881 cagactaacg aatacggctt ccttgagact ccgtatcgta aagtgaccga cggtgttgta
     5941 actgacgaaa ttcactacct gtctgctatc gaagaaggca actacgttat cgcccaggcg
     6001 aactccaacc tggatgaaga aggccacttc gtagaagacc tggtaacttg ccgtagcaaa
     6061 ggcgaatcca gcttgttcag ccgcgaccag gttgactaca tggacgtatc cacccagcag
     6121 gtggtatccg tcggtgcgtc cctgatcccg ttcctggaac acgatgacgc caaccgtgca
     6181 ttgatgggtg cgaatatgca acgtcaggcc gttccgactc tgcgcgctga taagccgctg
     6241 gttggtactg gtatggaacg tgctgttgcc gttgactccg gtgtaactgc ggtagctaaa
     6301 cgtggtggtg tcgttcagta cgtggatgct tcccgtatcg ttatcaaagt taacgaagac
     6361 gagatgtatc cgggtgaagc aggtatcgac atctacaacc tgaccaaata cacccgttct
     6421 aaccagaaca cctgtatcaa ccagatgccg tgtgtgtctc tgggtgaacc ggttgaacgt
     6481 ggcgacgtgc tggcagacgg tccgtccacc gacctcggtg aactggcgct tggtcagaac
     6541 atgcgcgtag cgttcatgcc gtggaatggt tacaacttcg aagactccat cctcgtatcc
     6601 gagcgtgttg ttcaggaaga ccgtttcacc accatccaca ttcaggaact ggcgtgtgtg
     6661 tcccgtgaca ccaagctggg tccggaagag atcaccgctg acatcccgaa cgtgggtgaa
     6721 gctgcgctct ccaaactgga tgaatccggt atcgtttaca ttggtgcgga agtgaccggt
     6781 ggcgacattc tggttggtaa ggtaacgccg aaaggtgaaa ctcagctgac cccagaagaa
     6841 aaactgctgc gtgcgatctt cggtgagaaa gcctctgacg ttaaagactc ttctctgcgc
     6901 gtaccaaacg gtgtatccgg tacggttatc gacgttcagg tctttactcg cgatggcgta
     6961 gaaaaagaca aacgtgcgct ggaaatcgaa gaaatgcagc tcaaacaggc gaagaaagac
     7021 ctgtctgaag aactgcagat cctcgaagcg ggtctgttca gccgtatccg tgctgtgctg
     7081 gtagccggtg gcgttgaagc tgagaagctc gacaaactgc cgcgcgatcg ctggctggag
     7141 ctgggcctga cagacgaaga gaaacaaaat cagctggaac agctggctga gcagtatgac
     7201 gaactgaaac acgagttcga gaagaaactc gaagcgaaac gccgcaaaat cacccagggc
     7261 gacgatctgg caccgggcgt gctgaagatt gttaaggtat atctggcggt taaacgccgt
     7321 atccagcctg gtgacaagat ggcaggtcgt cacggtaaca agggtgtaat ttctaagatc
     7381 aacccgatcg aagatatgcc ttacgatgaa aacggtacgc cggtagacat cgtactgaac
     7441 ccgctgggcg taccgtctcg tatgaacatc ggtcagatcc tcgaaaccca cctgggtatg
     7501 gctgcgaaag gtatcggcga caagatcaac gccatgctga aacagcagca agaagtcgcg
     7561 aaactgcgcg aattcatcca gcgtgcgtac gatctgggcg ctgacgttcg tcagaaagtt
     7621 gacctgagta ccttcagcga tgaagaagtt atgcgtctgg ctgaaaacct gcgcaaaggt
     7681 atgccaatcg caacgccggt gttcgacggt gcgaaagaag cagaaattaa agagctgctg
     7741 aaacttggcg acctgccgac ttccggtcag atccgcctgt acgatggtcg cactggtgaa
     7801 cagttcgagc gtccggtaac cgttggttac atgtacatgc tgaaactgaa ccacctggtc
     7861 gacgacaaga tgcacgcgcg ttccaccggt tcttacagcc tggttactca gcagccgctg
     7921 ggtggtaagg cacagttcgg tggtcagcgt ttcggggaga tggaagtgtg ggcgctggaa
     7981 gcatacggcg cagcatacac cctgcaggaa atgctcactg ttaagtctga tgacgtgaac
     8041 ggtcgtacca agatgtataa aaacattgtg gacggcaacc atcagatgga gccgggcatg
     8101 ccagaatcct tcaacgtatt gttgaaagag attcgttcgc tgggtatcaa catcgaactg
     8161 gaagacgagt aattctcgct caaacaggtc actgctgtcg gggtaaaacc cggcagcgga
     8221 ttgtgctaac tccgacggga gcaaatccgt gaaagattta ttaaagtttc tgaaagcgca
     8281 gactaaaacc gaagagtttg atgcgatcaa aattgctctg gcttcgccag acatgatccg
     8341 ttcatggtct ttcggtgaag ttaaaaagcc ggaaaccatc aactaccgta cgttcaaacc
     8401 agaacgtgac ggccttttct gcgcccgtat ctttgggccg gtaaaagatt acgagtgcct
     8461 gtgcggtaag tacaagcgcc tgaaacaccg tggcgtcatc tgtgagaagt gcggcgttga
     8521 agtgacccag actaaagtac gccgtgagcg tatgggccac atcgaactgg cttccccgac
     8581 tgcgcacatc tggttcctga aatcgctgcc gtcccgtatc ggtctgctgc tcgatatgcc
     8641 gctgcgcgat atcgaacgcg tactgtactt tgaatcctat gtggttatcg aaggcggtat
     8701 gaccaacctg gaacgtcagc agatcctgac tgaagagcag tatctggacg cgctggaaga
     8761 gttcggtgac gaattcgacg cgaagatggg tgcggaagca atccaggctc tgctgaagag
     8821 catggatctg gagcaagagt gcgaacagct gcgtgaagag ctgaacgaaa ccaactccga
     8881 aaccaagcgt aaaaagctga ccaagcgtat caaactgctg gaagcgttcg ttcagtctgg
     8941 taacaaacca gagtggatga tcctgaccgt tctgccggta ctgccgccag atctgcgtcc
     9001 gctggttccg ctggatggtg gtcgtttcgc gacttctgac ctgaacgatc tgtatcgtcg
     9061 cgtcattaac cgtaacaacc gtctgaaacg tctgctggat ctggctgcgc cggacatcat
     9121 cgtacgtaac gaaaaacgta tgctgcagga agcggtagac gccctgctgg ataacggtcg
     9181 tcgcggtcgt gcgatcaccg gttctaacaa gcgtcctctg aaatctttgg ccgacatgat
     9241 caaaggtaaa cagggtcgtt tccgtcagaa cctgctcggt aagcgtgttg actactccgg
     9301 tcgttctgta atcaccgtag gtccatacct gcgtctgcat cagtgcggtc tgccgaagaa
     9361 aatggcgctg gagctgttca aaccgttcat ctacggcaag ctggaactgc gtggtcttgc
     9421 taccaccatt aaagctgcga agaaaatggt tgagcgcgaa gaagctgtcg tttgggatat
     9481 cctggacgaa gttatccgcg aacacccggt actgctgaac cgtgcaccga ctctgcaccg
     9541 tctgggtatc caggcatttg aaccggtact gatcgaaggt aaagctatcc agctgcaccc
     9601 gctggtttgt gcggcatata acgccgactt cgatggtgac cagatggctg ttcacgtacc
     9661 gctgacgctg gaagcccagc tggaagcgcg tgcgctgatg atgtctacca acaacatcct
     9721 gtccccggcg aacggcgaac caatcatcgt tccgtctcag gacgttgtac tgggtctgta
     9781 ctacatgacc cgtgactgtg ttaacgccaa aggcgaaggc atggtgctga ctggcccgaa
     9841 agaagcagaa cgtctgtatc gctctggtct ggcttctctg catgcgcgcg ttaaagtgcg
     9901 tatcaccgag tatgaaaaag atgctaacgg tgaattagta gcgaaaacca gcctgaaaga
     9961 cacgactgtt ggccgtgcca ttctgtggat gattgtaccg aaaggtctgc cttactccat
    10021 cgtcaaccag gcgctgggta aaaaagcaat ctccaaaatg ctgaacacct gctaccgcat
    10081 tctcggtctg aaaccgaccg ttatttttgc ggaccagatc atgtacaccg gcttcgccta
    10141 tgccgcgcgt tctggtgcat ctgttggcat cgatgacatg gtcatcccgg agaagaaaca
    10201 cgaaatcatc tccgaggcag aagcagaagt tgctgaaatt caggagcagt tccagtctgg
    10261 tctggtaact gcgggcgaac gctacaacaa agttatcgat atctgggctg cggcgaacga
    10321 tcgtgtatcc aaagcgatga tggataacct gcaaactgaa accgttatta accgtgacgg
    10381 tcaggaagag aagcaggttt ccttcaacag catctacatg atggccgact ccggtgcgcg
    10441 tggttctgcg gcacagattc gtcagcttgc tggtatgcgt ggtctgatgg caaagccgga
    10501 tggctccatc atcgaaacgc caatcaccgc gaacttccgt gaaggtctga acgtactcca
    10561 gtacttcatc tccacccacg gtgctcgtaa aggtctggcg gataccgcac tgaaaactgc
    10621 gaactccggt tacctgactc gtcgtctggt tgacgtggcg caggacctgg tggttaccga
    10681 agacgattgt ggtacccatg aaggtatcat gatgactccg gttatcgagg ggggtgacgt
    10741 taaagagccg ctgcgcgatc gcgtactggg tcgtgtaact gctgaagacg ttctgaagcc
    10801 gggtactgct gatattctcg ttccgcgcaa cacgctgctg cacgaacagt ggtgtgacct
    10861 gctggaagag aactctgtcg acgcggtaaa agtacgttct gttgtatctt gtgacaccga
    10921 ctttggtgta tgtgcgcact gctacggtcg tgacctggcg cgtggccaca tcatcaacaa
    10981 aggtgaagca atcggtgtta tcgcggcaca gtccatcggt gaaccgggta cacagctgac
    11041 catgcgtacg ttccacatcg gtggtgcggc atctcgtgcg gctgctgaat ccagcatcca
    11101 ggtgaaaaac aaaggtagca tcaagctcag caacgtgaag tcggttgtga actccagcgg
    11161 taaactggtt atcacttccc gtaacaccga actgaaactg atcgacgaat tcggtcgtac
    11221 caaagaaagc tacaaagtgc cttacggtgc ggtactggcg aaaggcgatg gcgaacaggt
    11281 tgctggcggc gaaaccgttg caaactggga cccgcacacc atgccggtta tcaccgaagt
    11341 aagcggtttt gtacgcttta ctgacatgat cgacggccag accattactc gtcagaccga
    11401 cgaactgacc ggtctgtctt cgctggtggt tctggattcc gcagaacgta ccgcaggtgg
    11461 taaagatctg cgtccggcac tgaaaatcgt tgatgctcag ggtaacgacg ttctgatccc
    11521 aggtaccgat atgcctgcgc agtacttcct gccgggtaaa gcgattgttc agctggaaga
    11581 tggcgtacag atcagctctg gtgacacctt ggcgcgtatt ccgcaggaat ccggcggtac
    11641 caaggacatc accggtggtc tgccgcgcgt tgcggacctg ttcgaagctc gtcgtccgaa
    11701 agagccggca atcctggctg aaatcagcgg tatcgtttcc ttcggtaaag aaaccaaagg
    11761 taaacgtcgt ctggttatca ccccggtaga cggtagcgat ccgtacgaag agatgattcc
    11821 gaaatggcgt cagctcaacg tgttcgaagg tgaacgtgta gaacgtggtg acgtaatttc
    11881 cgacggtccg gaagcgccgc acgacattct gcgtctgcgt ggtgttcatg ctgtgactcg
    11941 ttacatcgtt aacgaagtac aggacgtata ccgtctgcag ggcgtaaaga ttaacgataa
    12001 acacatcgaa gttatcgttc gtcagatgct gcgtaaagct accatcgtta acgcgggcag
    12061 ctccgacttc ctggaaggcg aacaggttga atactctcgc gtcaagatcg caaaccgcga
    12121 actggaagcg aacggcaaag tgggtgcaac ttactcccgc gatctgctgg gtatcaccaa
    12181 agcgtctctg gcaaccgagt ccttcatctc cgcggcatcg ttccaggaga ccactcgcgt
    12241 gctgaccgaa gcagccgttg cgggcaaacg cgacgaactg cgcggcctga aagagaacgt
    12301 tatcgtgggt cgtctgatcc cggcaggtac cggttacgcg taccaccagg atcgtatgcg
    12361 tcgccgtgct gcgggtgaag ctccggctgc accgcaggtg actgcagaag acgcatctgc
    12421 cagcctggca gaactgctga acgcaggtct gggcggttct gataacgagt aatcgttaat
    12481 ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta tgggtgttat tcagagggca
    12541 aattcttcac ggaaaatatt aagaaaggca gggcgaaacg aggaagaagc ttttcgccct
    12601 ggacaacatt cctgctgacg gcactaccat aaaaaaacac aacgctttca acaaccttgc
    12661 gtttttcatc ttccgtagcg gctcggaata aatttacgtt cttacattcc gtctcatagt
    12721 ctttgcgagg cgcgtcccag atagctgtcc cagtctttcc ataccggctg caaaccctga
    12781 gcggttaaca cggcagcaac cgcttccggt ctgcgatcgt cgtgcggtga gaactgttcc
    12841 aactcggggt gattatcggc atagccacct ggctgcgttt tcgagaaggc gctgacgtta
    12901 ttgatcgcca gcggaataac gcgatcgcga aaccacggtg attcccgcgt ggagagtgac
    12961 agttcaatct ccggtgcaag cagtcggaag gcgcagatgg tttgcactaa ctggcgttca
    13021 tccataatcg acgcaggctc aatgccgcca gtacacgggc gcaggcgcgg aaaggagaca
    13081 gagtaacggc tttgccagta atgctgttgc agccatagca aatgttcggc aaccatatag
    13141 cagtcaacgc gccagttgtc ggaaaggcca attagcgcgc cgaggcctat cttatcaatc
    13201 cccgcacgcc ccagccgatc cggcgtttcc agccgccaga agaagtcctg ttttttgcct
    13261 ttcaaatgat ggcgggtata agtcgcctcg tgatatgtct cctgataaac catcacccca
    13321 tccagaccaa gttgctttaa ctcggcgtat tccgtctccg ccagcggttg cacttccatt
    13381 tgtagtgaag agaactgttc acgcagggcg gggagatgac gacgaaagta atccatcccc
    13441 actttcgcct gatgttcacc agtgactaat agcagatgtt caaagcccat ctcccgtata
    13501 gcggcgcttt ccctggcaat atccgcttca tccagcgttt tgcgcttgat gcgattactc
    13561 atggaaaatc cgcagtacgt gcagtcgtta gcgcaaagat tggaaagata aagcgggacg
    13621 tagaaactaa ctgtgttgcc aaatcgctga cgggtcagac gctgcgcctg ttgggccagt
    13681 tgttccagat agccactggc ggcaggcgat aacagcgcca tcatgtcgtc gcgggtgagt
    13741 tgcgaggcat ttagcgcccg ctctacgtca gcagccgttt tgccgttgat acgcaggcgg
    13801 atgtcgtccc agtccagttg tcgccagcga ttgctgaagg ttttcatgcc gatgcctcca
    13861 gaaatccggt cagcgggctg gtggcatgag caaaataact gcggctgccc ggtccggact
    13921 gacgtgccag caggcctact tctaccgcca gacgaaatgc cttcgccatg ttgacgggat
    13981 cgtccgcgac ggcaatcgcc gtattcacta acaccgcgtc ggcccccatt tccagcgcct
    14041 gcgtggcatg gctgggaacg ccgatgccag catcgacaac caccggaact gtggcctgct
    14101 ggataataat ctccagcatg gcacgggttt ccagtccctg attcgagcca atcggcgcgc
    14161 cgagcggcat caccgctgca cagccgactt cttccagacg tttacacaat accggatcgg
    14221 ccccgcagta aggcagcacg acaaatccct gttgtaccag cgtttcggcg gctttcaggg
    14281 tttcgatggg atcgggcaac agccagcggg cgtcagggtg aatctccaat tttagccagt
    14341 ttgtgcctaa cgcttcacgt gccagatggg cggcgaaaat ggcttcttcc gctgttttcg
    14401 ccccggaagt attcggcagc agggccacac ccgccgcgat aagcggttcg aggatagcgt
    14461 cgttgtgctg gcgcaagtcg acacgtttca tcgccagtgt caccagctgg ctgccggaag
    14521 cgcggatcgc ctccaccatc agttgtgaag aagcgaattt ccctgtgccg gtaaacagat
    14581 gtgaatcaaa cgttttgtcc gcaatacgta acatctcaac cccctgcaat aacctgaaaa
    14641 agcaggatct ggtcgccatc ctgcacgata tgttgcgtcc actgctcacg cgggacgatt
    14701 tgctgattaa tcgccagagc cgcgcccgct tgtcgttggt cgagttgctc cagtagttcg
    14761 tgaacagttt gcccggcggc gcactgcatc ggttgatcgt taaacaggat ctgcattgct
    14821 tcctccgcat accgggcaac cactggcgcg gcgcaacgcc aggctgcgcc actggctcga
    14881 cttaccgtcg aacagtcgga gttctcccgc aggcgtctct ataccgctta ataacttaat
    14941 ggcttccagt gcctgcaaag tgcccatcac cccgaccacc gggccaacca cgcccgccgt
    15001 gcggcagttg cgttctggct gctggttatc tggccacagg cagcggtaac acccctgctc
    15061 ccagggcggt gtcagtacca tcaactgacc gccaaatccg accgcgctgg cggtgataag
    15121 cggcgtgttg agtgccacgc aggcggcatt aatctcctgg cgagtcgcca tattgtcggt
    15181 acagtcgagc accacatcgg cttgtgcaac cgcatctttc aacgcctcac ccgttaaccg
    15241 ttgttgtaat gctgtcagtt gaatgtcggg attcaactgt gtcagtcgct gttggctgac
    15301 ctgcgatttc gggctgttga tatcttcagt ggtaaagagg atttgtcgtt gcagattgct
    15361 taaatgcaca tcgtcgtcat ctgccagtac cagcgtcccg acgccagccc ctgccaggta
    15421 tagcgcagca ggtgtaccca gcccgccaag accaataatc agcacctggc tgtcgagcag
    15481 tttttgctgc ccgtcaagag cgatatcgtc gagcagaatt tggcggctat aacgcataaa
    15541 gtcacggtca ttcatcgcca actcctgcaa tttccagcaa ctgtgccgtt gccaaacgcc
    15601 agtctgcggc ttgagtaatg gcgctgacga cggcgatact gccgacaccc gttgctatca
    15661 ccgcaggcgc gcgtgccaga ctgataccgc caatcgccac ggtgggataa tccgccaggc
    15721 gctcaacatg ccgtgccaac tgttccagcc cctgcggtgc agaaggcatc tgtttggttt
    15781 gcgtcgggaa cacatgtccc agcgcgatat aagaggggcg tgctgccagc gcgacgtcga
    15841 tttccatatc gtcatgtgtc gaaacgccca gccgcaggcc tgccgcgcgg atggcattga
    15901 gatcggtggc ttgcaaatct tcctgcccca aatggacgcc atacgcctga tgcttgatcg
    15961 cgagccgcca gtaatcgttg ataaacaatc gcgcgttata gcggcggccc agcgcaattg
    16021 ccgccacgac atcggcttcc acctcttcat cgcgccgatc tttgatgcgt agctggagag
    16081 tacgtacgcc tgcatccaac agacgttcga tccactgtac gctgtccacc accgggtaca
    16141 gtcctaaacg aaaaggtaca ggaggaaaat caggctgata catcacgctt cctccttatg
    16201 caggtagatt tctccgcctc tggcacggaa gttctccgac atatccgcca ttcccacttc
    16261 aatggtttgc gcggcggcgt aatcgcgtac ttcctggctg attttcatcg agcagaattt
    16321 tggcccacac atggagcaaa aatgggcgac tttgccggac tcttggggca gggtttcatc
    16381 gtgataagcg cgggcggtaa acgggtcgag ggccagatta aactggtctt cccagcgaaa
    16441 ttcgaagcgg gctttcgaca tggcgttatc gcgaatttgc gcgcccggat gcccttttgc
    16501 caggtcagca gcgtgggcgg caatcttata agtgataagc ccctgcttaa catcttcttt
    16561 attgggcaga cccagatgct cttttggcgt tacgtaacag agcatcgcgc agccaaacca
    16621 gccaatcatc gccgcaccaa tccccgacgt gaagtggtca tagcccggtg caatatcggt
    16681 agttagcggc cccagagtgt aaaacggcgc ttcgtggcag tgctctaact cctcggtcat
    16741 attgcggcgg atcatctgca tcggcacatg gcctgggcct tcaatcatca cctgtacgtc
    16801 atattcccag gcgattttgg tcagttcgcc cagcgtatgc aattcggcaa actgcgcttc
    16861 atcgttggcg tcctgaatag aaccggggcg cagaccgtcg cccagcgaca gcgaaacgtc
    16921 ataagcggca cagatttcac aaatttcgcg gaagtgttga tagaggaaat tttcctgatg
    16981 atgggagagg caccatttcg ccataatcga accgccgcga gagacgatac cggtcaggcg
    17041 tttcgcggtc atcggcacat agcgcagcag tacgcccgca tggatagtga agtaatccac
    17101 accttgctcg gcctgttcca gcagcgtgtc gcggaacgct tcccaggtaa gatcttcggc
    17161 gatcccgtta accttctcca gcgcctggta gatcggcact gtaccgatcg gcaccggact
    17221 gttacgcaaa atccactcgc gggtttcgtg aatatagcga ccggtggaga gatccatcac
    17281 cgtatccgct ccccagcgcg tggaccatac cagcttttcc acttcttctt cgatggaaga
    17341 ggtgaccgcc gagttgccga tattggcgtt aacttttacc aggaagttac ggccaataat
    17401 catcggctcc gattccggat gattaatgtt ggccgggata atcgcacgtc cggcagcaac
    17461 ttcatcacgg acaaattccg cagtgatatt ttccggcaga tgtgcgccaa agctcattcc
    17521 cggatgctgg tggcgtaaaa cctcgctacg gatgcgctcg cggcccatat tctcgcggat
    17581 ggcgatgaat tccatttccg gcgtgatgat gccctggcgg gcgtagtgca gttgggtgac
    17641 acggcgtcct gttttggcgc gttttggcgt taataagccg ctaaaacgta gctcgtcgag
    17701 accatcatct gccagccgcg ttctggtgta atcggaactg cgcacggtaa gttcttcggt
    17761 atcggcgcgc gcatcgatcc acggctggcg tagttttgcc agcccttgct gcacgttaat
    17821 ggcgatctgc ggatcaccat acgggccgga ggtgtcgtag accggaatcg cttcgttttc
    17881 ttcgtactgc ggctgttctt tgctaccgcc aattagcgtc gggctaagct ggatctcacg
    17941 catcggcacg cgcacgccgg ggtgtgtgcc agtgatataa atgcgttttg agttgggaaa
    18001 ggcggtgcct tccagggtgt cgataaaatg ttgggcccgg gcgcgttgtt cgcggcgggt
    18061 cagttttgtt gcagacatag ctcattccaa aaagttaagg acgtggcttg tcagacgacg
    18121 gatgaagcaa gagacgatcg ccgcaaaggc gatgcgatag cagattaact cttgttccct
    18181 tcgcaggtat tagcctgatc aggttccgcg gatcccgaat aaacggtctc agccagttaa
    18241 ggcactccga caagaaatta gcccccacaa acggggcgtt gaatgtaaat tacgcgttaa
    18301 cagcgcagaa ctcaagcagg atgtttgacg cgggcggcgt caagcaccag cagaatcagc
    18361 ttatcttcca gcacaaagcg cgcttccagc gcctcgccga tgtcagataa aacctgttga
    18421 aactcaaggt aattatcatg atcgatggcg gtttccaggc tggaatcgta gtaatccata
    18481 atctgttggg tattggcttc gagttgcggc caaatcttcg cggcgcgcgc gagttgcccg
    18541 ttgccttcca gcttatgaag aatgcgctca taaatactga aatgtccggc agacaagtaa
    18601 tcgaccaggc tctgacaaaa atcatcaagg gctttttcgt ttagcctcat gtacgattct
    18661 ttgccaggct taatgccaac cagattgtag taagccacga gcagatgctt acgtacatgt
    18721 agccagcgat caaccagttt gttacttcct ctgacgcgct ccgtcaggtt atcgagctgg
    18781 ttaagcatga ttgactccgc aagtttgtat tcaaaaactg ctcagtgaga aatgtaaaaa
    18841 ccatgttaaa catgccagtg atgcaaaggt agtgcaagag ctatggatcg tataattgaa
    18901 aaattagatc acggctggtg ggtcgtcagc catgaacaaa aattatggtt gccgaaggga
    18961 gaattgccat atggcgaagc ggcaaatttc gatcttgtgg gtcagcgcgc actacaaatc
    19021 ggcgaatggc agggggaacc tgtttggtta atacaacagc agcggcgtca cgatatgggg
    19081 tcggtacgtc aggtcattga tctcgatgtt gggctgtttc aactggccgg acgtggcgtg
    19141 caactggcgg agttttaccg atcgcataaa tactgtggtt actgcgggca tgaaatgtat
    19201 ccgagcaaaa ccgaatgggc gatgctgtgt agccattgcc gtgagcgtta ctacccgcaa
    19261 atcgccccct gcattattgt tgccatccgt cgcgatgatt cgctcctcct cgcccagcat
    19321 acccgccatc gtaacggtgt ccatacagta cttgccggat tcgtcgaagt gggcgaaacc
    19381 ctcgagcagg cagtcgcgcg ggaagtgatg gaagagagcg gaattaaagt taaaaacttg
    19441 cgttacgtga cttctcagcc gtggccgttt cctcagtctt taatgaccgc gtttatggcg
    19501 gaatatgaca gcggcgacat cgtgatcgac ccgaaagaat tgctcgaggc gaactggtat
    19561 cgctatgacg atttgccgtt actcccgccg cccggcaccg tagcgcgccg tctgatagaa
    19621 gatactgtgg cgatgtgtcg ggcagagtat gagtgatgat acactgaccg cctgacgcac
    19681 taaggaacag ccaaaatgac cgaacttaaa aacgatcgtt atctgcgggc gctgctgcgc
    19741 cagcccgttg atgtcactcc agtatggatg atgcgccagg cgggtcgcta tctaccggaa
    19801 tataaagcca cgcgcgccca ggcgggcgat tttatgtcgc tgtgcaaaaa cgccgagctg
    19861 gcgtgcgaag tgactttgca accgctgcgt cgctacccgc tggatgcggc gatcctcttt
    19921 tccgatatcc tcaccgtgcc ggacgcgatg gggttagggc tctattttga agccggagaa
    19981 ggtccgcgtt ttacctcgcc agtcacctgc aaagccgacg tcgataaact gccaattccg
    20041 gacccggaag atgagctggg ttacgtgatg aacgcggtgc gtaccattcg tcacgaactg
    20101 aaaggcgaag tgccgctgat tggtttttcc ggcagcccgt ggacgctggc aacctacatg
    20161 gtggaaggcg gcagcagcaa agccttcacc gtgatcaaaa aaatgatgta tgccgatccg
    20221 caggcgctgc acgctctgct cgataaactg gcgaaaagcg tcactttgta tctgaatgcg
    20281 cagattaaag ccggtgctca ggcagtgatg attttcgaca cctggggcgg cgtgcttacc
    20341 gggcgcgatt atcaacagtt ctcgctctat tacatgcata aaattgttga tggtttactg
    20401 cgtgaaaacg acggtcgccg cgtgccagtc acgctgttta ccaaaggcgg cggacagtgg
    20461 ctggaagcca tggctgaaac cggttgcgat gcgctgggcc tcgactggac aacggacatc
    20521 gccgatgcgc gccgccgtgt gggcaataaa gtcgcgttgc agggtaatat ggatccgtcg
    20581 atgctgtacg cgccgcctgc ccgcattgaa gaagaagtag cgactatact tgcaggtttc
    20641 ggtcacggcg aaggtcatgt ctttaacctt ggtcacggca ttcatcagga tgtgccgcca
    20701 gaacatgctg gcgtgttcgt ggaggcagtg catcgactgt ctgaacagta tcaccgctaa
    20761 gagcctgatc catcaggcta ttttatttgc cattttgagg ccgggcagtg ctcaaaatcc
    20821 tcacgtacta cgtgtacgct ccggtttttc cgcgctgtcc ggcctcaaac tgccttcaac
    20881 aattacgcct gttgaaccag gctctaagga gcgattatgg atctcgcgtc attacgcgct
    20941 caacaaatcg aactggcttc ttctgtgatc tgcgaggatc gactcgataa agatccaccg
    21001 gatctgatcg ccggagccga tgtcgggttt gagcagggcg gagaagtgac gcgagcggcg
    21061 atggtgctgc tgaaatatcc ctcgcttgag ctggtggagt ataaagttgc ccgcatcgcc
    21121 accaccatgc cttacattcc aggttttctt tccttccgcg aatatcctgc gctgctggca
    21181 gcgtgggaga tgctgtcgca aaagccggat ttagtgtttg tcgatggtca tgggatctcg
    21241 catcctcgcc gtcttggcgt cgccagccat tttggcttat tggtggatgt gccgaccatt
    21301 ggcgtggcga aaaaacggct ctgcggtaaa ttcgaaccgc tctccagcaa accgggcgcg
    21361 ctggccccac tgatggataa aggcgagcag ctggcctggg tctggcgcag caaagcgcgc
    21421 tgtaacccgt tgtttatcgc taccggccat cgggtcagcg tggacagcgc gctggcgtgg
    21481 gtacaacgct gcatgaaagg ctatcgtctg ccggagccaa cgcgctgggc ggacgcggtg
    21541 gcctcggaac gtccggcgtt cgtgcgctat acagcaaatc agccctaatt caggtaaact
    21601 gcggataatt tccgtatttg agaactcaac atgttacaaa acccaattca tctgcgtctg
    21661 gagcgcctgg aaagctggca gcatgtcact ttcatggctt gcttatgcga acgcatgtac
    21721 cccaattacg ccatgttctg ccagcaaacc ggttttggtg atgggcaaat ttaccgtcgt
    21781 attctcgatc tcatctggga aacgctgacc gttaaagacg caaaagtaaa tttcgacagc
    21841 caactggaga aatttgaaga agcgattcct tcagccgacg atttcgatct gtacggcgtt
    21901 tatccggcaa tcgatgcctg cgtggcgtta agtgaactgg tccattcgcg tttgagtggt
    21961 gaaacgctcg aacacgcggt ggaagtgagt aagacctcca tcacgaccgt tgcgatgctg
    22021 gaaatgactc aggctggtcg cgaaatgggc gatgaagagc ttaaagaaaa cccagctgta
    22081 gagcaagaat gggacattca gtgggaaata ttccgacttt tagctgagtg cgaagaacgc
    22141 gatatcgagc tgataaaagg ccttagggca gacctgcgtg agtcgggtga gagcaatatt
    22201 ggtataattt ttcagcaata agaccagaaa acgtgattta acgcctgatt tgtcgtacct
    22261 ggagtcttcc ctttcgcccc ccgtctggtc tacatttggg gggcgaaaaa aagtggctat
    22321 cggtgcgtgt atgcaggaga gtgctattct ggcatttccg tcgcactcga tgcttagcaa
    22381 gcgataaaca cattgtaagg ataacttatg aacaagactc aactgattga tgtaattgca
    22441 gagaaagcag aactgtccaa aacccaggct aaagctgctc tggagtccac tctggctgca
    22501 attactgagt ctctgaaaga aggcgatgct gtacaactgg ttggtttcgg taccttcaaa
    22561 gtgaaccatc gcgctgagcg tactggccgc aacccgcaga ccggtaaaga aatcaaaatt
    22621 gccgcagcta acgtaccggc atttgtttct ggcaaggcac tgaaagacgc agttaagtaa
    22681 gattgcgtgg cagtgaacag ttttaacgaa ggggtggttt cacccctttt gtctttctgg
    22741 cgtcgatcat tgatgctggc aggtgcgctg tttctcactg cctgtagcca caactcttca
    22801 cttcctccct ttaccgccag tggatttgct gaagaccagg gcgcggtacg catctggcga
    22861 aaagacagcg gcgataatgt gcatctgctt gccgtgttta gcccgtggcg cagtggcgat
    22921 accacgacgc gagagtatcg ctggcagggc gataacctca cgctcatcaa tatcaatgtt
    22981 tacagcaaac cgccggtgaa cattcgcgcg cgttttgacg atcgcggcga tctgagcttt
    23041 atgcaacgtg aatccgatgg ggaaaagcag cagctttcta acgaccaaat cgatttatac
    23101 cgttatcgtg ctgatcagat ccgccagatt agcgatgcct tacgtcaggg gagagtcgtg
    23161 ctgcgccagg ggcgctggca tgcgatggaa cagaccgtga ccacctgcga agggcaaacc
    23221 attaaacctg atttagattc gcaggcgata gcgcatatcg agcgccgcca gagccgctct
    23281 tctgttgatg tcagcgtggc atggctggaa gcgcccgaag gttcgcaatt actgttagtg
    23341 gcaaactctg atttctgtcg ctggcaaccc aacgagaaaa cgttctgatt taccagtggc
    23401 ccatacccat atgaccgcca ccaccgcagc cgccgtagcc catgcccatt ccggctccgc
    23461 gcggaatacc cgcttcagcc atcgcgatat ctcgtttcac ccgtaactca tctaacgact
    23521 gacgcaaatt ctccatctct ttggcgaccg cgttaatttt gctgctatcc ggtgggttcg
    23581 cggctaacag ggcattgtat tcataacgct tcgtcaccag ttgctgttgc agtgcgctgc
    23641 tttgagcgta aaagtcatta tggattttct gccacgctgt ctgttgttcg ctggtcaaag
    23701 gcgcggcatt ttgctgccac ataccgtgtc cgccgtgagc aaatgcagat gtcgatccca
    23761 tcgccattgc tgaaagcgcc atcattacca gggcaatttt cgtgttccgt ttcatggtta
    23821 atcctccagt ggttgtctta cttcgggtat tgcatcttcc gtgccaacga tgaaacgctg
    23881 atatgacggg taatctggca tgataaacga gtaaaaatga ctcgcctgct gcgggtagcg
    23941 agtcattttt actcattgaa acttaagcct ttgtgttaca gcgcagggta agcgctgata
    24001 aaagatggca tgatttctgc tgtcagaaag ggatgagcag gcaaagaaga agatgcgttt
    24061 tatgcaacgt tctaaagact ccttagctaa atggttaagc gcgatcctcc ccgtggtcat
    24121 tgttgggctg gtagggttgt ttgcggtgac ggtgattcgc gattatgggc gcgagactgc
    24181 cgccgccaga caaacgctgc tggaaaaagg cagtgtactt attcgcgctc ttgaatccgg
    24241 ctcgcgcgtc ggcatgggga tgcgcatgca tcatgcgcag cagcaggcct tactggaaga
    24301 aatggccggg cagcctggag tacgttggtt tgcggtcacg gatgaacaag gaacaatcgt
    24361 gatgcatagc aactccggca tggtgggaaa acagctttat tcccctcagg aaatgcagca
    24421 gttacatccg ggagatgaag aagcgtggcg gcggatcgat agcgcagacg gcgagcctgt
    24481 tctggaaatt tatcgccagt ttcaaccgat gtttgctgct ggaatgtacc ggatgcgcca
    24541 tatgcagcag tatgccgcga caccacaagc aattttcatc gctttcgacg ccagtaatat
    24601 tgtgagtgcc gaagatcgtg agcagagaaa caccctgatt attctcttcg ccctggcgac
    24661 ggtcttgctg gcaagcgtat tgtcattctt ctggtatcgc cgctatctgc gctcgcgcca
    24721 gcttctacaa gatgaaatga agcgcaaaga gaagctggtg gcgctggggc atcttgcggc
    24781 aggcgttgcc cacgaaatcc gtaacccact ttcctcgatt aaaggactgg cgaaatactt
    24841 tgccgagcgc gcgcctgcag ggggagaagc gcatcaactg gcgcaggtga tggcgaaaga
    24901 ggccgaccgt ttaaaccgcg tggtaagcga gttgctggaa ctggttaagc caacgcatct
    24961 ggctttgcag gcggtggatc tcaacacgct gattaaccac tcattacagc tggtaagcca
    25021 ggatgcaaac agccgggaga tccagttacg ctttaccgcc aacgacacat taccggaaat
    25081 tcaggccgat ccggacaggc tgactcaggt cctgttgaat ctctatctca atgctattca
    25141 ggcgattggt cagcatggcg tgattagcgt gacggccagc gaaagcggcg cgggcgtgaa
    25201 aatcagcgtt accgacagcg gtaagggaat tgcggcagat cagcttgaag ccatcttcac
    25261 tccgtacttc accaccaaag ccgaaggcac cggattgggg ctggcggtcg tgcataatat
    25321 tgttgaacaa cacggtggta caattcaggt cgcaagccag gagggaaaag gctcaacgtt
    25381 caccctctgg cttccggtca atattacgcg taaggaccca caaggatgac gcacgataat
    25441 atcgatattc tggtggtgga tgatgacatt agccactgca ctattttgca ggctttactg
    25501 cgcggctggg gctataacgt cgcgctggcg aacagcgggc gacaggcgct ggagcaggtg
    25561 cgggaacagg tttttgatct tgtgctttgc gatgtgcgaa tggcggagat ggacggcatc
    25621 gccacgctga aagagatcaa agcgttaaac ccggcaattc cggtgctgat tatgactgcg
    25681 tactccagcg tcgagacggc ggtagaggca ctgaaaactg gggcgcagga ttatctcatc
    25741 aagccgctgg atttcgataa cctacaggcg acgctggaaa aggcgctcgc gcatacgcac
    25801 agtattgatg ctgaaacacc tgcggtgact gccagccagt tcggtatggt cggtaaaagc
    25861 ccggcgatgc aacacctgct cagtgaaatc gccctcgtcg cgccatcgga agccacggtg
    25921 ctgatccacg gcgattccgg caccggtaaa gagctggtcg ccagggcgat tcacgccagt
    25981 agcgcacgta gcgaaaaacc gctggtaacg ctcaactgtg cggcactcaa cgaatccttg
    26041 ctggaatctg aattgttcgg tcacgaaaaa ggggcgttta ctggagccga taaacgccgg
    26101 gaagggcgct ttgttgaggc ggacggcggc acgctgtttc tcgatgaaat tggcgatatc
    26161 tcgccgatga tgcaggtgcg tctgctgcgt gcgattcagg agcgcgaagt tcagcgtgtc
    26221 ggtagcaacc agactatctc ggttgatgtc cggctgattg cggcgaccca tcgcgatctt
    26281 gccgcagagg tgaatgccgg gcgttttcgc caggatctct actatcgcct gaatgtggtg
    26341 gcgattgaag taccatcgct gcggcaacgg cgggaagata tccctctgct ggctggccat
    26401 tttcttcagc gctttgccga gcgtaatcgc aaggcggtaa aaggctttac gccacaggcg
    26461 atggatttgc tgatccacta cgactggccg ggaaatattc gtgaactgga aaacgcggtg
    26521 gaacgggcgg tggtgctgct gaccggggaa tatatttccg aacgcgagct gccgctggcg
    26581 attgccagta cgccgatccc gctggggcaa agtcaggata ttcagccgct ggtggaagtg
    26641 gaaaaagagg tgattctggc ggcgctggag aaaacgggcg gcaacaaaac cgaagccgcc
    26701 cgtcagttag ggatcacgcg caaaacgcta ttggcaaaac tgtcgcgtta gttctgctcg
    26761 cgttcgatgg cgcgccaacc gatatctttc cggcagaagc aatcatccca gtggatatcg
    26821 gtcattaagg cgtaggcgcg tttctgcgct tctgccacgg tatgacccag cgcggtgacg
    26881 cacagtacgc gcccgccgct ggttaccacc tgctcgtcat ccgccagttt tgtgcccgcg
    26941 tggaacactt tgccgtcagc cacttcttcc agcggcaggc cgtggatcac atcgccggtg
    27001 cggtaatcgc ccggatatcc acccgcagcc atcaccacgc tgagagaagc acgttcatcc
    27061 cactctgacg ttttctcgtc cagtttgcct tcacaggccg ccaggcagag ctcaaccaga
    27121 tcggacttca tgcgcagcat aatcggctgg gtttccggat cgccaaagcg gcagttaaat
    27181 tcaataacct tcggattgcc ctgtttgtcg atcatcaggc ccgcatagag aaaaccggtg
    27241 taggtgttgc cttccgccgc catgcctttc acggttggcc agatgatacg ttccatggtg
    27301 cgctgatgaa cttcatcggt tactaccggc gcgggggagt aagcgcccat cccgccggtg
    27361 tttggtccgt tatctttatc gcctacgcgt ttgtgatcct ggctggtagc catcggcagc
    27421 acatgctcgc cgtccaccat cacgataaag ctcgcttctt cgccatcgag gaactcttcg
    27481 ataacgatgc gatgacccgc gtcgccaaaa gcgttgcccg ccagcatatc gtgaacagcc
    27541 gcttccgctt cttccagcgt catcgccacg ataacgcctt tcccggcagc cagaccgtcc
    27601 gctttaatga cgattggcgc gcctttctca cgcagataca ccagcgcagg ttctacctcg
    27661 gtgaagttct ggtattccgc cgtagggatc ttatggcggg ccaggaaatc tttggtaaac
    27721 gcttttgagc cttccagttg ggccgcacct gcggttgggc cgaagatttt cagcccggcg
    27781 gcgcggaagg tatcgaccac gcctttcacc agcggcgctt ccgggccgac gatggtcaga
    27841 tcaatctttt cgttttgtgc gaaatccaac agcgccggga tatcggtcac gccgatagca
    27901 acgttttgca gcgcgggttc cagcgcagtg cctgcattac ccggagcagc aaaaacagtc
    27961 tcaaccagcg gcgactgggc cgctttccag gccagcgcgt gctcgcgccc gccgttacca
    28021 atcactaata ctttcatcta ttgctccatt aatggcggaa gtggcgcatg tcggtgaaga
    28081 gcatcgcaat accgtgctcg tcggcggcgg caatcacttc gtcatcacgg atggaaccgc
    28141 caggctggat tacgcaggtc acgcccgcag cggcggcggc atcaataccg tcgcggaacg
    28201 ggaagaacgc gtcagaagcc atcgaggaac ctttcacttc caggccttca tcggcagctt
    28261 taataccggc gattttcgcg gagtacacgc ggctcatctg gcccgcgcca ataccgatgg
    28321 tcatattgtt tttggcatag acgatagcat tggatttcac gaatttcgcc actttccagc
    28381 agaacagcgc atcacgcagt tcctgttcgg tcggctgacg ttgggtgacg acgcgcagtt
    28441 cttccgcacc gaccatcccc aggtcacgat cctgaaccag cagaccgccg ttcacgcgtt
    28501 tgaaatcaag acccggaaca cgctcgcccc actgaccgca ggtcagaacg cgtacgttct
    28561 gcttggcggc ggtgattttc agggcttctt cgctggcgga aggcgcaata atcacttcga
    28621 caaactgacg agaaatgatg gcctgtgcgg tttccgcatc cagctcgcgg ttaaaggcaa
    28681 tgatgccgcc gaatgcggag gttgggtcgg ttttgtacgc gcgatcgtaa gcatcaagaa
    28741 tggaattgcc gatagctaca ccgcaagggt tggcgtgctt cacaatcaca catgccggct
    28801 cggcgaactc tttcacgcac tccagcgccg catcggtatc ggcgatgttg ttataagaga
    28861 gggctttacc ctgaacctgg gttgcggtag caacggaggc ttctttcaca ttctcttcta
    28921 tatagaaggc agcctgctgg tggctgttct cgccgtaacg catatcctgc ttcttaatga
    28981 agttcaggtt cagcgtgcgt gggaagcgac cggcggcttc tttgctttca ccgtggtacg
    29041 ccggaaccat gctgccgaag tagttggcaa tcatgctgtc gtaggcggca gtgtgttcga
    29101 aggctttgat ggcgaggtcg aaacgggttg caagcgtcag cgatccttcg ttgtcatcca
    29161 tctctttaat aatggcgtcg tagtcgctgc tcttcaccac gattgcgaca tctttatggt
    29221 tcttagcggc ggagcgcacc atggtcgggc cgccgatatc gatgttctca accgcatctt
    29281 ccagcgagca gccttcacga gcaacagtct gggcgaacgg atacaggtta acaaccacca
    29341 tatcgatagg ctggatctga tgttcttcca taatggcatc gtcctggccg cgacggccca
    29401 gaatgccacc atgtactttc ggatgcaggg tcttcacgcg tccatccatc atctccggga
    29461 aaccggtgta atcggaaact tcggttaccg gcagaccttt ttctgctaac agacgggcag
    29521 tgccccctgt agacagcagc tccacaccgc gtgcggaaag tgcctgggcg aattcgacga
    29581 taccggcttt gtcagaaaca ctgagcagag cgcggcggac tggacgacgt tgttgcatgg
    29641 taaatcccct gggtttgact attacagaga gcgttagctg aatttttcgc gaaaaactca
    29701 gctaacgccc ctaacggggc atccttattt ttcgcccgca ttgtaacgaa aacgtttgcg
    29761 caacgctcgc gaatttttct ctttcaatgg tgatcacaat tttgacagtg gttaccgtgg
    29821 gaaaaataca gaaattacat tgatgattgt ggataactct gtgcgtaaaa aggtataaag
    29881 cggggttttg ctggggaatg cagcagtcag tcatttttct gcaatttttc tattgcggcc
    29941 tgcggagaac tccctataat gcgccttcat cgacacggcg gatgtgaatc acttcacaca
    30001 aacagccggt tcggttgaag agaaaaatcc tgaaattcag ggttgactct gaaagaggaa
    30061 agcgtaatat acgccacctc gcgacagtgc gctaaagcgc gtcgcaactg ctcttta
//
LOCUS       DAJTUI010000042        29933 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTUI010000042 DAJTUI010000000
VERSION     DAJTUI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.042"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..452)
                     /gene="wcaD"
                     /locus_tag="OO464_002053"
     CDS             complement(<1..452)
                     /gene="wcaD"
                     /locus_tag="OO464_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR6227208.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISK"
     gene            complement(427..1644)
                     /gene="wcaC"
                     /locus_tag="OO464_002054"
     CDS             complement(427..1644)
                     /gene="wcaC"
                     /locus_tag="OO464_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR6227209.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(1641..2129)
                     /gene="wcaB"
                     /locus_tag="OO464_002055"
     CDS             complement(1641..2129)
                     /gene="wcaB"
                     /locus_tag="OO464_002055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR6227210.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2132..2971)
                     /gene="wcaA"
                     /locus_tag="OO464_002056"
                     /pseudo
     CDS             complement(2132..2971)
                     /gene="wcaA"
                     /locus_tag="OO464_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3064..5226)
                     /gene="wzc"
                     /locus_tag="OO464_002057"
     CDS             complement(3064..5226)
                     /gene="wzc"
                     /locus_tag="OO464_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR6227211.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5229..5672)
                     /gene="wzb"
                     /locus_tag="OO464_002058"
     CDS             complement(5229..5672)
                     /gene="wzb"
                     /locus_tag="OO464_002058"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR6227212.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(5678..6817)
                     /gene="wza"
                     /locus_tag="OO464_002059"
     CDS             complement(5678..6817)
                     /gene="wza"
                     /locus_tag="OO464_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR6227213.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7126..7275
                     /locus_tag="OO464_002060"
     CDS             7126..7275
                     /locus_tag="OO464_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227214.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7476..9059
                     /locus_tag="OO464_002061"
     CDS             7476..9059
                     /locus_tag="OO464_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6227215.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9511..11364)
                     /gene="asmA"
                     /locus_tag="OO464_002062"
     CDS             complement(9511..11364)
                     /gene="asmA"
                     /locus_tag="OO464_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6227216.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11386..11967)
                     /gene="dcd"
                     /locus_tag="OO464_002063"
     CDS             complement(11386..11967)
                     /gene="dcd"
                     /locus_tag="OO464_002063"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6227217.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12059..12700)
                     /gene="udk"
                     /locus_tag="OO464_002064"
     CDS             complement(12059..12700)
                     /gene="udk"
                     /locus_tag="OO464_002064"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6227218.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13018..13260
                     /locus_tag="OO464_002065"
                     /pseudo
     CDS             13018..13260
                     /locus_tag="OO464_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13259..14200
                     /gene="mdtA"
                     /locus_tag="OO464_002066"
                     /pseudo
     CDS             13259..14200
                     /gene="mdtA"
                     /locus_tag="OO464_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14200..17322
                     /gene="mdtB"
                     /locus_tag="OO464_002067"
                     /pseudo
     CDS             14200..17322
                     /gene="mdtB"
                     /locus_tag="OO464_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17323..20388
                     /gene="mdtC"
                     /locus_tag="OO464_002068"
     CDS             17323..20388
                     /gene="mdtC"
                     /locus_tag="OO464_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6227219.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20389..21804
                     /locus_tag="OO464_002069"
     CDS             20389..21804
                     /locus_tag="OO464_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6227220.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            21801..23204
                     /gene="baeS"
                     /locus_tag="OO464_002070"
     CDS             21801..23204
                     /gene="baeS"
                     /locus_tag="OO464_002070"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6227221.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNLEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23201..23923
                     /gene="baeR"
                     /locus_tag="OO464_002071"
     CDS             23201..23923
                     /gene="baeR"
                     /locus_tag="OO464_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6227222.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24114..24446
                     /locus_tag="OO464_002072"
     CDS             24114..24446
                     /locus_tag="OO464_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR6227223.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24593..25954
                     /gene="yegQ"
                     /locus_tag="OO464_002073"
     CDS             24593..25954
                     /gene="yegQ"
                     /locus_tag="OO464_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6227224.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26296..26613)
                     /locus_tag="OO464_002074"
     CDS             complement(26296..26613)
                     /locus_tag="OO464_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227225.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27019..27918
                     /gene="yegS"
                     /locus_tag="OO464_002075"
     CDS             27019..27918
                     /gene="yegS"
                     /locus_tag="OO464_002075"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR6227226.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28000..28773)
                     /locus_tag="OO464_002076"
     CDS             complement(28000..28773)
                     /locus_tag="OO464_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6227227.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(28879..29919)
                     /gene="gatD"
                     /locus_tag="OO464_002077"
     CDS             complement(28879..29919)
                     /gene="gatD"
                     /locus_tag="OO464_002077"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6227228.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         6889 a         7969 c         7755 g         7320 t
ORIGIN      
        1 tatttcgaaa tcgactccat aatactgctg ccaccggtga ggataatttg cgccatctcc
       61 accgccgcca gcgccaccac cagtccgacc accagataaa agaaacgtaa tatcttacga
      121 tggttgtgtg gcgaaattgt tttaaaacga atactccaca ccatgccaat aatgatcaca
      181 atatagacaa acagcatagt tgaagtgacg tatttaccgg catccaacga ctgaccaaac
      241 aggtagttaa acgccgtgag tcccgcgcca atccctaagg caatcatcaa ttttttaatg
      301 ctgatgcgtt ctaaaaacag cagtaacaag acaggtaaaa aagtgacgat ggtgatgggg
      361 aaactttcgc caagctgggc gattttgacg ttaaccagca ggtagatcag tggcagcagc
      421 aggtagctac agattctgat agaagttgac atactcctcc agcatctgtt gtccactgta
      481 ggcggcgcga ctgcgttggc tgaactcagc cagcgtggta ccaaatatcg cctgcgcgat
      541 ttccggtttg cttaactgta ccagttgcag cacctcttct tcactgacgg ttttaccacc
      601 ggatttttgc agcacttccc gcgccgcatc gctatgggtg gcaatcaccg gcacgccaat
      661 cgatagcgcc tcacacaaaa tcagcgggta gttatcgaca cgagaactga acaccagcgc
      721 atccatctga ttgagcgcgc tcatcaactt gcgcttgtca gtttcaaagc cgtgattaac
      781 cacgttgcca gcggtgaacg gcgagaactt accaaaggta tgcagttcga ttttgtcgcc
      841 cagcgccatc atctcacgca ccagttgctg gttagttttg ccgtcgtaac gcaggtcatg
      901 cgccaccact gcgattttcg gcttgccctg agtttcgcgc accggaggca gatctgccag
      961 aatcgcttcg gttgccatat caatgccatt attgataatc cggcaacgcc ctggcccgta
     1021 cagactattg aaagcgtcag ccacatgctg gctgggggaa ataaactgac agcccagcgc
     1081 cagcatctcg cggaataact ggcgtttgcc cgccaccagc tggtgcgcgc gatcaatctt
     1141 caccggcgga tagttattta aggtcgggca tttctggcag cccgttttcc agccttcgca
     1201 accgtcggta aaggcgcagc gtccggttac actccagtgg tcgtgcagcg tccagaccag
     1261 ggtgacatcc ggtttatggt ttttcacttt ttcgcaaaag cgcaccacgc tgttaagatt
     1321 caaccagtag ctgtgcagca catgaaaatg caggaccacc ggacccggtg tgcgagtaat
     1381 ggtgcgatat aactcattga aattgccgaa cagatcgcga ttaaacagac gaaacaacgc
     1441 aatgttcgcc atcgcggtca tccgcggcgt atgtttgatg acctgcggat agttctgatg
     1501 gctgacgctc tctttgccgc ctttgccgta gccgtagaca aaatgtgacg ccagcccctg
     1561 ttgcagcgca cgctggtgga gatctaacgc cacacctgct gccccgcctt ccgccagtcg
     1621 cacattaaat tgcagaatat tcatttaatc accttcactc gcgctttttc tcccaccacc
     1681 agcgcgttgt ccgggacaga gtcgagcacc acgctgcccg cacccacggt gacgttgtta
     1741 ccaagcgtga tatcaccaag aataatgacg ttggcaccga gttcgacgcc gttgccaatg
     1801 tgtggacatg ccatgttatc ggcaccccga ttgccgatag tgacgccgtg gcgaatggtg
     1861 aaatcatccc ccgccaccac gtttttattg atcactacgg cgtaaccgtg atggatagta
     1921 aagcggcggc caatggtcgc ggcggcctgg atttcataac cgaaaaagca ttcggtgata
     1981 atgcgataca gcaccagcag cggggccgcc cacagattgt tgaggacgtt ctttttgcgc
     2041 cacaccgaac aaaaatgagc gacgcgatag gcaagtacca tgcagcacgg gcgtaaactc
     2101 cagctgttgg cgcgcagatc ttccagcatc tttagcgccc ccgaatacca tcagccagac
     2161 gtttgccgtt acgcaccgac agcagcgtca acaaggtgcg ccaggtcata cgtttattgc
     2221 ggatctggta gagggtaaac agctgatatt ttttactggc gcggtcgaat ttgtctttgt
     2281 gcttgcggta aaagtggaag tatccggaga attttttcgg tgacgaggtg atctgcattt
     2341 cgccgtgatt gatatgcagg atctgcgtcg cctcttccac tttccacggt tcgccgtact
     2401 ccaccaccat ccgcaggaat atgtcgtaat cctgtgctgc tttcagttcg gtatcgaaca
     2461 aacactcttt gaaacgccat gcccaggtaa agacctgatt gccaatgata ttacgtttat
     2521 agaacaggcg gcgtgagtac ggcgatttgg gatacagtgg taagctcgcc ggttgggaat
     2581 aaacttctcc ctagcaaacg tagtcgttag cgtacaaaaa ggcgtgtgtg accagttgct
     2641 gtttatgggc gaggaagacg ctcaggcggt tgggcgtcca ttcatcatca tcatcgatcc
     2701 cggtgatgta ttccccttgc gccagcataa tcgcctggtt acgcaccgcg cacgccccgc
     2761 tgttaatgtc gttgtggatg taagtgatac gcggatcgtt gagggcagtg acgtactgtt
     2821 gcagttgttc ccaggaggtg gagcaatcat ccacgatgat catctcccag ttgctgtagt
     2881 cctggcgcag gaccgatttt atcgcccgaa tcgccagttg ttggcggttc caggttggca
     2941 tatagattga gatcagcgga ttgtctttca taggttgttc tcccggctta tgagctggtt
     3001 tgcttgccgg atgcggcgtg agcgccttat ccggcctacg gggcggtgcg aatgcaggcg
     3061 tcgttatttc gcatccgact tatattcgta ttcgtaatag ccataatcct gatacgcgct
     3121 ggcgcggcgg aagatggagt tcagaatcac ccctttcacc ggaataccgt tttgctcaaa
     3181 gcggctcaga ctggtttcca cttctttcaa tgtgttgacc gcataacgcg ccaccattaa
     3241 cgtggttccg acatgacgac caacaattgc cgcatcggtc actgccagaa tcggcggcgt
     3301 atcaatcaac accaggtcat agtttttact cgcccagttc accagttcgg caaagcgttc
     3361 gctcattaac agttcagaag gatttggcgg cacctgaccg cgcgggatca ggtcaaattt
     3421 ggcaatagag gtcggtttag cagcggtagt aatatcgccc tgaccaatca gaatctccga
     3481 cagcccattg acattattgg tgcccaacag ctcgtgggtg tagcctttgc gcatatcgca
     3541 gtcgatcaac aacacgcgtt tattggtctg gctgatcacc gccgccaggt tggcgcagac
     3601 aaaggtttta ccgattgacg ggctaacccc ggtcatcatc aacacattgt tctgcgcctg
     3661 catcatcgcg aagtgcagac tggtacgcag actacggatg gcttcaatcg ccagatcggt
     3721 tggattcccc actgccagta gctggctctg tttatagcgt ttaacccctt tgatggtttt
     3781 gacgctatcg cgcgctttct gccactccga cagcgggatg ctggcataga cgctgattcc
     3841 gtgttcttcc agcacttgcg ggctttcgat gccgcgatta aacaacgagc gcagcagcac
     3901 acccacgata gagagcatca ggccaaggat aatcgcgccg aggataatca gccctttctt
     3961 cggttttagc acgccaggct gggtgattgc cgggtcaata atgcgcacat cgccgacggt
     4021 gctggcttcg gtgattttca gctcctgctc tttattcagc agttgcatat agacctgctg
     4081 accagactcg acatcgcggg tcagacggac aatttcctgc tggtttttcg gcatcgccgt
     4141 tacgcgaccg ttaagtttgg ctttctcttc ttccagcgcc tgacgtttct ccagcagcgt
     4201 gcggtacgcc gggtgaactt tggtgtacag cttggagatt tccgcctctt taaaggtcag
     4261 ttcgttcaac tgcgcgtcga tgttcaccat cgaatcgagc accgctttcg cttcaagcgg
     4321 cagatcaaca gaatctttat cctgacggaa ggcattcagt ttgttttcgg caacatcgag
     4381 gcggctacgt acttccggta actgttgcgc gaggaaagcg aggcttttcg acgcttccgc
     4441 cgatttgcgc tcaatatttt gttcctgata gttacgagcg atgctgttaa gaatgtcgcg
     4501 gatctgttcg cgatcttcac cggtataagt caggctcagt acgcctgcgt ctttgccgtt
     4561 ctccgttacc gtcaggctgt tttgcagttg gttgatcatc cccaacgtgg agtatttggt
     4621 gacggtaaac tcactgcccg ggcgggcgtg aatggcttca accatcagcg tgacgccttc
     4681 ttttttcagc atctggcccg cttgcccacg ggcgctaaag ccgccatcac tgctcagggt
     4741 gtagtttttg tcatccagca cattaagcgt aaacacctga tccgccatct ctttcgggcg
     4801 gttaaaggta gtcactttca ccgtctcgtt ctgacgcccc atcaggcgat cccagcccgc
     4861 cccgaaaatc gggaacgtgt ttttgctcac cgcaatatcg aggtcgagat cgtccaccgt
     4921 tttaccaagc accagacgcg aacgaatcaa ctggatctcg gcgtccgatg caggcggttt
     4981 gttggctaat gccgagccga tatcctgcac cagcgaattg ccgctgtttt gctcgatttg
     5041 taccagtgcg tcggcactat aaatcggcgt ggcgaagaaa gtgtaaacca cggcacagag
     5101 ggcaaatacg acggtgatgc caatcaccca ccagcgcgct tcaatgacgg tgccgaccag
     5161 gcgaccaata tcgatttcat cactgcccgt taccggagcg gcatgttgtt ttactttttc
     5221 tgtcattctt atacctgctc tgcgttcaat gcctgcgccc actggcgggc agaccgttca
     5281 agtaatgtgt acaccgctgc aaacgtttcc cggcttttgt gatacggatc ggggatttca
     5341 cattcgttat cccagtgacc aaacagcatc actttgccgc gcatctccgg tgccatctcg
     5401 cataagcgtt cgatatggcg cttatccatg gtcaaaatca ggtcatagtt gcgacacagg
     5461 cggcggctga tttgtcgtgc acagtggcct tccagagaca gttgatgttc tgcggcgacg
     5521 ctgatagcgg taggatcagc gcccttaccg accagcgcgc cgagtccagc ggactccact
     5581 ttcagttccg ggtgataacg ttgcagtaag cgttccgcca tcggggaacg gcaaatattg
     5641 ccgacacaga caactaagat gttgttaaac atgacgatta ccagttatga atgtcgctgg
     5701 ctgtatccgt catgtaacgg acaccgctaa tagttggcag caactgattg atcagacggt
     5761 tccagcggga aaccggggcg gtggtgacat acaccacgtc ataaggttgc aggcggaatt
     5821 ctgtcgccat caccagcgac gtcgcatcgg acatatccag ctggtagata ttggcaatct
     5881 tgccgttacg cccgccctcg cctttcagcg gacgaatgac aaagatgccg ctggcgttgg
     5941 aggtggtcat gtcgatacct tcggcattgg ccagagcttc agtcagggtc atgccgctaa
     6001 agtccatttt cagggtgctc tgtttcttca cttcacccat cacaaatact ttcagatcat
     6061 cattacgcgg cacgtagaga atatcgccgg gataaagcag tcggttctga ttgaggtcgc
     6121 cgttttgcat cagcgcctgt aaagaaatgc gttcttcacg accattgtgt gttagcacca
     6181 cgttgcgcca gtcagcggtg tcggtcaggc cacctgcggc gttgatggcg tcgagaatgg
     6241 tcagtggtac gttggtgatc gcctgttgac cggatttatt cacctgacct gagatatagg
     6301 ccttttgtga gcggaaggcg gcgatattaa cgtccacctg cgggtcagcg atgtacgtcg
     6361 ctaagcgccc ggtaatatca ctgcggattt cagcgagcgt tttcccgact acgtgaacct
     6421 tgccgatata cgggtaaaac atagtgccgt caggctgtac ccagttgccg gtgtcgctgg
     6481 agctgcggta ctgaccagct ggcgtggtga gttccgggtg atcccagacg gtgacattaa
     6541 gaacgtcccc cggcccgacg cgatactggt aattcgcgat ctcgctttcc agcgtcatat
     6601 tggggcgcgc tacattcggg cgtgggcgta attggtcaat caggcgcggg gtcagcggat
     6661 aaacattcac cattttgtcg agatcgaaat cagcgtcctg ctgtttgatg acgtctttgc
     6721 ccatcgtcga catattgctg cccggaagta ctgtgcaacc gcttatcaag gttactgaca
     6781 ccaataatgg catcaatttc attttggatt tcatcattgt ttatttatca ctttggcaga
     6841 gtaattatcc tgtgcactat taatagcaat gtcgccatgc acatttacct tgcagttaat
     6901 tgaataaaaa tttaactggc atcagtccta aaaaaattga tttcatccgc aggctattga
     6961 cagaataatt cagactggtc tttcaggcat ccagacacgc taccgcccct ggctttttag
     7021 ctaccaatac actgattgag cttaattttt caaaccctct ctgcatacaa tcgttgatga
     7081 gaaagggtta ttgcggaaat taacttccga atataaggtg acattatggt aattgaatat
     7141 tggctcgcca ataatgcagg aggaagtgtt acagctaacg gaatagcagg caaggtaaca
     7201 attcggcaat tggctatttt taagaattat attaagtatc attcaatatg gtttttagga
     7261 gtttctttag gttgacaata tttaatattg cgtctccaca tgcgatattt cttaaataat
     7321 gttttattat taccgctttt aattcaggga taatgtgagg ttattaccct caaataatat
     7381 gagataaata gtgctgcaac attgcatttt tgccccgatt tatccatgat cgaattgtga
     7441 catttgtcat acaacgaata ggttttgtac ttactatgga atggattgcc gatccgtcta
     7501 tctgggccgg gttaatcacg ctgattgtga tcgaactggt cctcggcatt gataacctgg
     7561 tctttattgc catcctcgcc gaaaaactac cgccgaagca gcgcgaccgc gcacgggtta
     7621 ccgggctgct gctggcgatg ttaatgcgcc tgttactgct agcgtcaatc tcctggctgg
     7681 tcaccctgac tcaaccgctg ttcagcttcc gctcgtttac ctttagcgcc cgcgacttaa
     7741 tcatgctgtt tggtggtttc ttcctgttgt tcaaagccac gatggagctg aacgaacggc
     7801 tggaagggaa agacagcaat aatcccacac aacgcaaagg tgcgaagttc tggggcgtgg
     7861 tgacgcaaat tgtggtactg gacgccattt tctcactcga ctcagtgatt accgccgtcg
     7921 ggatggtcga ccatttactg gtcatgatgg ccgccgtggt tatcgctatc agcctgatgt
     7981 tgatggccag caagccgtta acgcaatttg ttaacagtca cccgaccatc gttattctct
     8041 gcttaagctt cctgttgatg attggcttta gcctggtggc agaaggtttc ggcttcgtca
     8101 ttccgaaagg ctacctgtac gctgccattg gtttctcggt gatgatcgag gcactcaatc
     8161 agttggctat ctttaaccgg cgacgttttc tttccgctaa tcagacgcta cgccagcgga
     8221 ctaccgaagc ggtaatgcgc ctgcttagcg ggcaaaaaga agatgcggaa ctggacgcca
     8281 aaaccgcgtc catgctggtg gatcatggaa accagcagat ctttaatccg caggaacggc
     8341 ggatgattga gcgggtactt aatcttaacc agcgtaccgt aagcagcatt atgacgtcgc
     8401 gccacgatat tgagcatatc gatctcaacg cgccggaaga agaaatccgc cagttgctgg
     8461 agcgaaatca gcatacgcgg ctggtggtta ccgacggtga tgacgcagaa gatttgctcg
     8521 gtgttgttca cgttatcgac ctgctacaac agtcactgcg cggcgaaccg ctcaacctgc
     8581 gggtgttgat tcgccagccg ctggtgttcc cggaaacctt gccgttgtta ccagccctgg
     8641 agcagttccg taatgcccgc acgcactttg cttttgtggt ggatgagttt ggctcggtgg
     8701 aagggattgt gacattaagt gacgtcactg aaaccattgc cggtaactta ccgaacgaag
     8761 tggaagagat cgacgcccgc catgatattc agaagaatgc ggacggttcc tggacggcga
     8821 atggtcatat gccgctggag gatctggtgc aatatgtgcc gctgccgctg gatgaaaaac
     8881 gtgaatatca caccattgcc gggctgttga tggagtattt gcagcgtatt ccaaaacctg
     8941 gcgaagaagt tcaggtgggg gattatttgc ttaaaacgtt gcaggtggaa agccatcgcg
     9001 tgcagaaggt gcagattata ccgctgcgta aggatggcga gatggagtac gaggtgtgat
     9061 ggtatcggcc attttaccca atgctgcgta acgccttata acttcagcaa tgaagcgttg
     9121 tctgtggtaa acggacatca cagcgggggt gtgggtccgg ctgtaaatcg ccgaatcgtt
     9181 tcctgtaggc tggggcagcc cacatggatg tgggctgagg gcgcgtttta cagggatgtt
     9241 acctcgcgcc cgtacccgat agccgaaagg aataagatga gggaaccgcg ccagcggcga
     9301 tttaccgccg ggagcccggg ttgccagggt ggtggcgatt gagccaccct ggcacgttca
     9361 ccggcttgag cgttccatag tagcgaggaa catcaagtga acggaacagc cttcgatctt
     9421 acatattcag aatataatag tgccgccgcg aacgcctgat gcgacgcttt acgcgtctta
     9481 tcaggcctac aggacaggtc acctggtaac ctacatcttc tccagcaact tcttcacatc
     9541 tttgccattg cgggaatctt tattccgctc ggcccagtca ttcaggcgac gtttcgcttc
     9601 gtcctgtaga tgtttgcgca gtaactgatc cacttgcagg ctgtaattga gttgctgcca
     9661 gttgccataa acccgcagcg gtactggcgt ttctttgagg aaatcaatca gtttgctttc
     9721 cccgttccag ccacccacga cccgaatatc aaactgggtg tcgcaggttt gatctgccag
     9781 attcaacatg ccttcccctg tcagcgccag cacaggcgat tgaccttgca tgtcgtttaa
     9841 cgtcacgacg ccatccttca acgtcaaatc ggtggtaaag cggtcaagcc gcgtcacgtt
     9901 atcgaagttt tcagcggcct tcacatcacc gccattacgc tctaccgctt gctgaatcat
     9961 ctgctggaag ttcatccctt ccatacgtgt gtcggtcatc tcaacatgcg cctgtccttg
    10021 ccagttgtgg cggaatgcgt cggcatctat gtcagcaccg gagaaatcac cagccagtga
    10081 catttttccg gtcaacgaaa tcggatagtt aaacgccttc agaatggtac caatctcaac
    10141 gttctccagc cgtggctgga agtttatccg cggatttatt gatgtcgcgt ccagcgtgcc
    10201 cggcagtgaa acctttccac cgttaagctt gccctgcagt tgagtaattt ccagcaaacc
    10261 cgacttgttg gtcatttgcg tggcaacatc tgtaaaattc attccgcgcc agcgcacgtt
    10321 actggcctgc aacaaaatat cagctgtaaa gccttgcagt ccctgatagg ccggttcatc
    10381 aatacgcgaa gaaatgaccg ggcgcggcaa cgttgattgg ctctgcccct gctgcgcggc
    10441 accgttttca ccattcgcgg tttcattaag cgggatgagg ttgtcaagat tcagttgcgg
    10501 gaattgcagt ctcagctgcc attccggttt ctctgtcagc gtgacctgtg cttgtccgct
    10561 cagcgtactg tcattggcgg tcaaactaat ttggttaaat gaaaggcgtt tatgtgactc
    10621 ctgccactgg gactggaaac tcccctgccc ctgaatacct tgttttggta aatcggcacc
    10681 ctgcaactgc cagttaattt gttcaatagc cgctgttaaa tcatgcggat aatcagaagc
    10741 atctaccgta ccgttaaggg atatcgtgag atcgcgctga tcgcgattaa cccgcccgga
    10801 gaactcaaat gagccacgat gttggggatc ttgttccatt tgcaggcgga tattgcggat
    10861 tgtcacctgc tcgtcatctt catgctggaa caccagaacg ctgtccgcca ccttaagact
    10921 ggatatatca aacgaccatc cgcgatcgtc tgacagatcc ggcaaggtat tgtcgcgcgg
    10981 tgcaaccgga gcgtcttcac tgcgcaccgc ttccgtctgc ggcgtcagtt ggatcactgc
    11041 cccttttagc atcacctgct taacgctcag ttgatgactc agtagtggta aaagcgccac
    11101 gtccagacgc atgttgtcgg cgcgaaccag tggctggctt gcgccctggg cggtgagaga
    11161 cattcgcccg gagaggatac taagctgcgg ccagacgtgc caacgcagtg gcccgtcgag
    11221 ctgcaattga taaccgctac gtgcagcaac ttgcttgacc atatagtcgc ggaaatcatt
    11281 cggattcacc agcaacacta acgcagataa cccggccacc agcacgacca agagtatcat
    11341 cagcgtcgtc agaaatcgtc tcatggtatc ctcaatgggc ctgaattagt ctttatcgat
    11401 tcggctggct accgcgccct gctggttgcg atatttcgca tcttcacggc ggttgtaagg
    11461 tcgcgccgcc gggccggaaa gcggctcaaa gctcagcgca ccaattaaca tgcccggacg
    11521 cagcgccagc ggcagcttac cggagttgta gaactccagc acaatgcaac cagaccagcc
    11581 cggatcgata cggtgcgcgg tgacgtgcac catcagcccc agacgcgcca aagaggaacg
    11641 cccgtccagc cagcccacca gatcggctgg cagcgtcacc gactccagtg tcaccgccag
    11701 cgccagctct cctgggtgaa gatagaacgc ctcgccctcg tcgagaacga tctcatcgct
    11761 catcacgcgg tcaagcgcgg cgctcacttc atctttggga ccgctcagat cgataaacgc
    11821 tgccgtgtga ccacggaagg tacgaaattt attgcccagg cgtacatcca ccgtcgcgcc
    11881 gttaatacgc tccactggcg gacgtgggtt gatcgacaaa cggccttcat caagccaggc
    11941 ttcaatatct cggtcacaca gacgcatggc attttctcct ttcgcacatc actcccttaa
    12001 cgccaattac gtcaagggca taccaactta tcactgaacg gtacacaatt tatcaagctt
    12061 attcaaagaa ctgacttatt ttcgctttca atatatcgat cgcgatgcgg tttttcccgc
    12121 cgcgcggcac gataatgtcc gcatattgtt tagaaggctc aatgaattgc aggaacatcg
    12181 ggcgcacggt tttttgatat tgcgccatca cggaatccat tgaacgccca cgctcgttaa
    12241 cgtcacgctt gatgcggcgc atcaggcaga tatccagcgg ggtatcaacg aaaatggaga
    12301 agttaagttc gtcacgcaaa cgcgcatccg tcagcaacaa aatgccttcg agaatgatga
    12361 ccttcttcgg ctcaaccgtc accgtttctt tcatacgcgt atgttcaaca tagctgtaaa
    12421 ccggcaggtc aattgccgag ccgcgtttca acgcttgtaa atgctcaagc aacaggctgt
    12481 gatccatcgc gctgggatgg tcgtagttgg tcttaacgcg ttcttccatc gacagatggc
    12541 tttgatcttt gtaatagcag tcttcgggaa ttacgccgat gtgttcatca ccgacttgct
    12601 cacgtaattc acgataaagg gtactggcaa taagactctt gccggaagcc gatgcgccag
    12661 cgataccgat aatgacgcac tgatgagact gatcagtcat atatttagcg acctgattaa
    12721 cctggatgtt aggaaggggg cgacgaagcg ccaaacgcgg caattatagg gatttcatcc
    12781 gtctgatacc agtcgaatag cgttgccgcg cgctcagagt taattgttga caaagaattc
    12841 ccggtggcaa attacgttga tcagttttat gtaaggtaaa aaatgttata cgcagttgcg
    12901 caaattatcc gcctttacgt cactttatga gcaattcgca tataaaatgt aaaacttttg
    12961 tactagcata aacacagaaa cgaatactgg cgacctggcc ttgcggataa agcggtaata
    13021 agcaaacaat cacagcatgt attaattgcc ctgccccacc cgctgcttca cctggtcagt
    13081 ttaggtttag tctcgtttat ctttaccctt gtctcgcttg agctttcgca gtttggcacc
    13141 caactcgccc cactgtggtt cccgacgtcc atcatgatgg tggcgtttta tcgccatgcc
    13201 gggcgcatgt ggccgggaat tgcgctgagc tgttcgctgg gaaatatcgc cgcatccatt
    13261 ccaggaaggc cagcaggtca aagcaggcga tttactggca gaaattgacc ccagccagtt
    13321 caaagttgca ttagcacaaa cccagggcca actggcaaaa gataaagcca cgcttgctaa
    13381 cgcccgccgt gatctggcgc gttatcagca actggcaaaa accaatctcg tctcccgtca
    13441 ggagctggat gcccaacagg cgctggtcag tgaaaccgaa ggcaccatta aggctgatga
    13501 agcaagcgtc gccagcgcgc agttgcaact cgactggagc cgcatcaccg caccagtcga
    13561 tggtcgcgtt ggtctcaagc aagttgatgt tggtaaccaa atctccagtg gtgataccac
    13621 cggaattgtg gtgatcaccc agacgcatcc tatcgattta gtctttaccc tgccggaaag
    13681 cgatatcgct actgtagtgc aggcgcaaaa agccggaaaa ccgctgatgg tagaagcctg
    13741 ggatcgcacc aattcgaaga aattaagtga aggcacgctg ttaagcctcg ataaccaaat
    13801 cgatgccaca accggtacga ttaaagtgaa agcacgcttt aataatcagg atgatgcgct
    13861 gtttcccaat cagtttgtta acgcgcgcat gttagtcgac accgaacaaa acgccgtagt
    13921 gatcccaaca gccgccctgc aaatgggcaa tgaaggccat tttgtctggg tgctgaatag
    13981 cgaaaacaag gtcagcaaac atctggtgac gccgggcatt caggacagtc agaaagtggt
    14041 gatccgtgca ggtatttctg cgggcgatcg cgtggtgaca gacggcattg atcgcctgac
    14101 cgaaggggcg aaagtggaag tggtggaagc ccagagcgcc accactccgg aagagaaagc
    14161 caccagccgc gaatacgcga aaaaaggagc acgctcctga tgcaggtgtt acccccgagc
    14221 agcacaggcg gcccgtcgcg cctgtttatt atgcgtcctg tcgccaccac gctgctgatg
    14281 gtggcgatct tactcgccgg gattatcggt tatcgcgccc tgcccgtttc ggcgctgccg
    14341 gaagtggact atccgaccat tcaggtggtc acgctctacc caggtgccag cccggatgtc
    14401 atgacctctg ccgttaccgc gccgctcgaa cgccagttcg ggcagatgtc tggcctgaaa
    14461 cagatgtcgt cgcaaagttc cggcggtgcg tcagttatca ctttgcagtt ccagctaaca
    14521 ttaccgctca atgtcgccga gcaggaagtg caggccgcga ttaacgctgc gaccaacttg
    14581 ttgccgagcg atctgcctaa cccgccggtt tacagcaaag tgaacccggc agatccgccg
    14641 atcatgacgc tcgccgtcac ctcaaccgcc atgccgatga cgcaagtgga agatatggtg
    14701 gaaacccgcg tcgcgcagaa aatctcgcag atttccggcg tcggcctggt gacgctttcc
    14761 ggcggtcagc gtccggctgt tcgcgtcaaa cttaacgctc aggcgattgc cgccctcggc
    14821 ctgaccagcg aaaccgtgcg caccgccatt accggcgcta acgttaactc ggcaaaaggt
    14881 agcctcgacg gcccttcccg tgcggtcacg ctttccgcga acgaccagat gcaatccgcc
    14941 gaagagtatc gccagctaat catcgcctac cagaacggcg cgccaattcg tctgggcgat
    15001 gtcgcaactg tagagcaagg tgcagaaaac agctggctcg gcgcgtgggc gaacaaagaa
    15061 caggccattg tgatgaatgt tcagcgccag cccggtgcta acattatctc caccgccgac
    15121 agcattcggc agatgctgcc acagctcact gagagtctgc cgaaatcggt gaaggtgaca
    15181 gtgctttccg atcgcaccac caatatccgc gcatccgtcg atgatactca gtttgaattg
    15241 atgatggcta tcgcgctggt agtcatgatt atctacctgt ttttgcgcaa tattccggcg
    15301 accatcattc ccggtgttgc tgtaccgctg tcgttaatcg gcactttcgc ggttatggtg
    15361 tttctcgatt tttcaatcaa taacctgaca ctgatggctt taactatcgc caccggattc
    15421 gtggtcgatg acgccatcgt ggtgatcgaa aacatttccc gctatatcga aaaaggcgaa
    15481 aaaccgttgg cggcggcgct caagggcgca ggtgaaatcg gctttaccat tatctcgctg
    15541 accttctcac tgattgcggt gttgatccca ctgctgttta tgggcgatat cgtcgggcga
    15601 ctgttccgcg aatttgctat taccctggcg gtagcgattt tgatctcagc ggtggtgtcg
    15661 ctgaccctga caccgatgat gtgcgcgcgg atgctcagcc aggagtcgtt gcgtaaacag
    15721 aacagcttct cccgtgcctc ggaaaaaatg ttcgacagga taatcgccgc ctatggtcgt
    15781 ggactggcga aagtgctgaa tcatccgtgg ctgaccttaa gcgtggcact cagcacgctg
    15841 ctgcttagcg tgctgctgtg ggtgttcatt ccgaaaggtt tcttcccggt acaggacaac
    15901 ggcattattc agggcacttt gcaggcaccg caatccagct cctttgccaa tatggcccag
    15961 cgacaacgcc aggtcgcgga cgtgattttg caggatccgg cagtgcaaag cctgacctca
    16021 tttgttggcg ttgatggcac taacccgtcg ctgaatagtg cacgtttaca aatcaacctc
    16081 aaaccgttgg atgaacgtga tgatcgggtg caaaaagtca tcgcccgtct gcaaacggcg
    16141 gtagataaag tgccgggcgt cgatctcttc ctgcaaccaa cgcaggacct gaccatcgat
    16201 actcaggtca gccgcaccca gtaccagttt accttgcagg ccacgtcact ggatgcgctc
    16261 agtacctggg tgccacagtt gatggaaaaa ctccagcaac tgccacagct ttctgatgtc
    16321 tccagcgact ggcaggacaa agggctggtg gcgtatgtca atgttgatcg cgacagcgcc
    16381 agccgtctgg ggatcagcat ggcggacgtc gataacgccc tgtacaacgc gtttggtcag
    16441 cgactgattt ccactattta tactcaggct aaccagtatc gcgtggtgct ggagcacaat
    16501 accgaaaata ccccaggcct cgcggcgctg gataccattc gcctgaccag cagcgacggt
    16561 ggcgtggtgc cgttaagctc aattgccaaa attgagcagc gttttgcgcc gctttccatc
    16621 aaccatctgg atcagttccc ggtaacaacc atctccttta acgtgccgga taactattcg
    16681 ctgggcgatg cggtgcaggc gattatggac accgaaaaga cgctgaatct gccggtggat
    16741 atcaccacgc aattctaggg cagcaccctc gccttccagt cggcgctggg cagcactgtc
    16801 tggctgattg tcgcggcggt ggtggcgatg tatatcgtgc tcggcattct gtacgagagc
    16861 tttattcacc cgatcaccat tctctcgacg ctacccaccg caggggtcgg cgcgctgctg
    16921 gcattaatga ttgccggtag cgaactggat gtgattgcga ttatcggcat tattttgctg
    16981 atcggtattg tgaagaagaa cgccatcatg atgatcgact tcgcgctggc tgctgagcgc
    17041 gagcaaggca tgtcgccgcg cgatgcaatc taccaggctt gtctgttgcg ttttcgtccg
    17101 atcctgatga ccaccctggc ggctctgctt ggcgcgctgc cgctgatgtt gagtaccggg
    17161 gtcggcgcgg aactgcgtcg tccgttaggt atcggcatgg tcggcggtct gattgtcagc
    17221 caggtgctga cgctgttcac cacgccggtg atttatctgc tgttcgaccg cctggcattg
    17281 tggaccaaaa gtcgctttgc tcgccatgaa gaggaggcgt aagtgaagtt ttttgccctc
    17341 ttcatttacc gcccggtggc gacgatttta ctgtcggtcg ccattaccct gtgcggcata
    17401 ctgggtttcc gtatgctgcc ggtcgccccg ctgccgcagg tcgattttcc ggtgattatg
    17461 gtcagcgcct cgctgcccgg tgcgtcgccg gagacaatgg cgtcttccgt tgccacgccg
    17521 ctggagcgct cacttgggcg cattgccggg gttagtgaaa tgacctccag cagttcgctc
    17581 ggcagcacgc gcattatttt gcagtttgat tttgaccggg atatcaacgg cgcagcgcgt
    17641 gatgtgcagg cggcgatcaa cgctgcacaa agtttgctgc ccagcgggat gcccagccgc
    17701 ccgacctatc gcaaagcgaa cccgtcggat gcgccaatta tgatcctcac gctgacatcc
    17761 gatacttatt cgcagggtga actgtacgat ttcgcctcga cgcagctggc tccgacgatt
    17821 tcgcaaatcg acggtgtcgg tgatgtcgat gtcggtggca gctcgctgcc cgccgtgcgc
    17881 gtcgggctga ccccgcaggc gctgtttaat cagggcgtgt cgctggacga cgtacgcact
    17941 gccatcagca atgccaacgt gcgtaaaccg cagggggcgc tggaagatgg cactcaccgc
    18001 tggcagatcc agaccaacga tgaactgaaa accgccgctg aatatcagcc gctgattatt
    18061 cactacaaca acggcggcgc ggttcgtctg ggcgatgtgg cgacggtgac tgactcagtg
    18121 caggatgtgc gcaacgccgg gatgaccaac gccaaaccgg ctattttact gatgatccgc
    18181 aaactgccgg aagccaatat tatccagacg gtagacagca tccgggcaaa attaccagag
    18241 ttgcaggaga ccattccggc ggcgattgat ctgcaaattg cccaggatcg ctcccccacc
    18301 attcgcgcct cgctggaaga agtcgagcaa acgctgatta tctcggtggc gctggtgatt
    18361 ctggtggtct ttttattcct gcgctcgggt cgcgccacta ttattcccgc cgttgcggtg
    18421 ccggtttcgc tgattggtac gtttgcggcg atgtacctgt gcggattcag tctcaataat
    18481 ctttcgctga tggcgctcac cattgctact ggtttcgtgg tggatgacgc cattgtggtg
    18541 ctggaaaaca ttgcccgtca tctggaagcg gggatgaaac cactacaggc cgcgctgcaa
    18601 ggtactcgcg aagtcggctt tacggtgctg tcgatgagtc tgtcactggt ggcggtgttc
    18661 ctgccgctgc tgttgatggg cggattgccg ggccgactgt tacgcgagtt tgccgtgacg
    18721 ctttctgtcg ccattggtat ttcgttgctg gtttccctga cgctaacgcc aatgatgtgc
    18781 ggctggatgc taaaagccag caagccgcgt gagcaaaagc gactgcgtgg ttttggccgc
    18841 atgttggtag ccctgcaaca aggctacggc aagtcgctga aatgggtgct caatcatacc
    18901 cgtctggtgg gcgtggtgct gcttggcacc attgcgctga atatctcgat cccgaaaacc
    18961 ttcttcccgg agcaggacac cggcgtactg atgggtggga tacaggcgga tcagagtatt
    19021 tcgtttcagg cgatgcgcgg caaattgcag gatttcatga aaattatccg tgacgatccg
    19081 gcagtggata atgtcaccgg ctttaccggc ggttcacgag ttaacagtgg gatgatgttt
    19141 atcaccctca agccacgcga cgaacgcagc gaaacggcgc agcaaattat cgaccgtctg
    19201 cgcgtgaagc tggcgaaaga accgggggcg aatctgttcc tgatggcggt acaagatatt
    19261 cgcgttggtg ggcgtcagtc gaacgccagc taccagtaca cgttgttatc cgacgacctg
    19321 gcggcactgc gcgaatggga gccgaaaatc cgcaaaaaac tggcgacgtt gccggaactg
    19381 gcggacgtga actccgatca gcaggataac ggcgcggaga tgaatctggt ttacgaccgc
    19441 gacaccatgg cacggctggg aatcgacgta caggccgcca acagtctgtt aaataacgcc
    19501 ttcggtcagc ggcaaatctc gaccatttac cagccgatga accagtataa agtggtgatg
    19561 gaagtggatc cgcgctatac ccaggacatc agtgcgctgg aaaaaatgtt cgttatcaat
    19621 aacgaaggca aagcgatccc gctgtcgtat ttcgctaaat ggcaaccggc gaatgcccca
    19681 ctatcggtga atcatcaggg attatcggcg gcctcgacca tctcgttcaa cctgccgacc
    19741 ggaaaatcgc tctcggacgc cagtgcggcg atcgatcgcg caatgaccca gcttggtgtg
    19801 ccttcgacgg tgcgcggcag ttttgccggc acggcgcagg ttttccagga gacgatgaac
    19861 tcgcaggtga tcctgattat cgccgccatc gccacggtgt atatcgtgct gggtatcctt
    19921 tacgagagtt acgtacatcc gctgacgatt ctctccaccc tgccctcggc gggcgttgga
    19981 gcgctgttgg cgctggagct gttcaatgcc ccgttcagcc taatcgccct gatagggatc
    20041 atgctattaa tcggcatcgt gaagaaaaac gccattatga tggtcgattt tgcgcttgaa
    20101 gcccaacggc acggtaacct gacgccgcag gaagctattt tccaggcctg tctgctgcgt
    20161 tttcgcccga ttatgatgac caccctggcg gcgctgtttg gcgcgctgcc gctggtattg
    20221 tcgggcggtg acggctcgga gctgcggcaa cccctggaga tcaccattgt cggcggtctg
    20281 gtgatgagcc agctcctgac gctgtatacc acgccggtgg tgtatctctt tttcgaccgt
    20341 ctgcggctgc gtttttcgcg taaacctaaa caaacggtaa ccgagtaaat gacagatctt
    20401 cccgacagca cccgctggca attatggatt gtggctttcg gcttctttat gcagtcgctg
    20461 gacaccacca tcgtaaacac cgcccttccc tcaatggcgc aaagcctcgg ggaaagtccg
    20521 ttgcatatgc acatggtcat tgtctcttat gtgctgaccg tggcggtgat gctgcccgcc
    20581 agcggctggc tggcggacaa agtcggcgtg cgcaatattt tctttaccgc catcgtgctg
    20641 tttactctcg gttcactgtt ttgcgcgctt tccggcacgc tgaacgaact gttgctggca
    20701 cgcgcgttac agggcgttgg cggtgcgatg atggtgccgg tcggcaggtt gacggtgatg
    20761 aaaatcgtac cgcgcgagca atatatggcg gcgatgacct ttgtcacgtt acccggtcag
    20821 gtcggtccgc tgctcggtcc ggcgctcggc ggtctgctgg tagaatacgc gtcgtggcac
    20881 tggatctttt tgatcaacat tccggtgggg attatcggtg cgatcgccac attgatgtta
    20941 atgccgaact acaccatgca gacgcggcgc tttgatcttt ccggcttttt attgctggcg
    21001 gttggcatgg cggtattaac cctggcgctg gacggcagta aaggtacagg tttatcgccg
    21061 ctggcgattg ctggcctggt cgcagttggc gtggtggcac tggtgcttta tctgctgcac
    21121 gccagaaata acaaccgcgc cctgttcagt ctgaaactgt tccgcactcg taccttttcg
    21181 ctgggcctgg cggggagctt tgccggacgt attggcagtg gcatgttgcc ctttatgaca
    21241 ccggttttcc tgcaaattgg cctcggtttc tcgccgtttc atgccggact gatgatgatc
    21301 ccgatggtgc ttggcagcat gggaatgaag cgaattgtgg tacaggtggt gaatcgcttt
    21361 ggttatcgtc gggtactggt agcgaccacg ctgggtctgt cgctggtcac cctgttgttt
    21421 atgactaccg ctctgttggg ctggtactac gttttgccgt tcgtcctgtt tttacaaggg
    21481 atggtcaact cgacccgttt ctcctccatg aacaccctga cgctgaaaga tctcccggac
    21541 aatctggcga gcagcggaaa cagcctgctg tcgatgatta tgcaattgtc gatgagtatc
    21601 ggcgtcacca tcgccgggct gttgctgggg ctttttggtt cgcagcatat cagcgtcgac
    21661 agcggcacca cacaaaccgt ctttatgtac acctggctta gcatggcgtt tatcatcgcc
    21721 cttccggcgt tcatctttgc cagagtgccg aacgatacgc atcaaaatgt agctatttcg
    21781 cggcgaaaaa ggagcgcgca atgaagttct ggcgacccgg tattaccggc aaactgtttc
    21841 tggcgatttt cgccacctgc attgtcttgc tgatcagtat gcactgggcg gtgcgtatca
    21901 gttttgagcg tggctttatt gattacatca aacacggcaa tgaacagcga ttacagctgt
    21961 taagtgatgc gcttggcgag cagtatgcgc agcatggtaa ctggcgcttc ctgcgcaaca
    22021 acgatcgctt tgtctttcag atcctgcgtt catttgaaca cgataatttg gaagataaac
    22081 ccggcccggg tatgccgccg cacggctggc gtacccagtt ctgggtggtc gatcaaaaca
    22141 acaaagtact ggttggtccg cgagcgccga ttccacctga cggtacacgg cgacccattc
    22201 tggtcaacgg tgcggaagtg ggcgcggtga ttgcctcccc tgttgagcgg ctgacccgca
    22261 atactgatat caatttcgat aaacaacagc ggcaaaccag ctggctgatc gtcgccctgg
    22321 caacgttact cgcggcgctc gccacctttc tgctggcgcg cggtttgctg gcaccggtaa
    22381 aacgacttgt cgatggcacg cacaaactgg cggcgggcga tttcactacc cgcgtaacgc
    22441 ccaccagtga agatgaactg ggcaaactgg cgcaagactt caaccagctc gccagcacgc
    22501 tggagaaaaa ccagcaaatg cggcgcgatt ttatggccga tatttctcac gaactgcgta
    22561 cgccattagc ggtgctgcgc ggtgaactgg aagccattca ggatggcgtg cgcaaattca
    22621 cgccggagac ggtggcgtct ttacaggcgg aggtcggtac actgaccaaa ctggttgacg
    22681 atcttcatca gttgtcgatg tctgatgaag gcgctctcgc ctatcaaaaa gcaccggtag
    22741 atttgatccc actgctggaa gtggcgggcg gcgcatttcg cgaacgattc gccagccgtg
    22801 gcctgaaact gcaattttcc ctgccagaca gtatcaccgt atttggcgat cgcgaccgtt
    22861 taatgcagtt attcaataac ttactggaaa acagcctgcg ctacactgac agcggcggca
    22921 gcctgaaaat ctctgccgag cagcacgaca aaacggtgcg cctgaccttt gccgacagtg
    22981 cgccaggtgt cagtgacgat cagctacaaa aattgtttga acgtttttat cgcaccgaag
    23041 gttcccgcaa ccgcgccagc ggcggttccg ggctggggct ggcgatttgc ctgaacattg
    23101 ttgaagccca taatggccgc attattgccg cccattcgcc ttttggcggg gtaagcatta
    23161 cagtagagtt accgctggaa cgggatttac agagagaagt atgaccgagt taccaatcga
    23221 cgaaaacaca ccgcgtattt tgatcgtgga agatgaaccg aagctggggc agttgctcat
    23281 tgattatctg cgtgctgcga gctatgcgcc gacgcttatc agccacggcg atcaggtact
    23341 ggcgtatgtg cgccagacac caccggatct gatcctgttg gatctgatgc tccctggcac
    23401 cgatggcctg acattatgcc gggaaattcg tcgtttttcc gatgttccga tcgtgatggt
    23461 gacggcaaaa atcgaagaga tcgaccgcct gctggggctg gagattggcg ccgatgacta
    23521 catctgcaag ccgtacagcc cacgggaagt ggtagcgcgt gtcaaaacca ttttgcgccg
    23581 ttgtaaaccg caacgcgaac ttcagcaaca ggatgccgaa agcccgttga ttatcgacga
    23641 aggtcgtttt caggcttcat ggcgcggtaa aatgcttgac ctgacgcctg cggaatttcg
    23701 tctgcttaaa acgctctctc acgaaccagg aaaagtgttc tcccgcgagc aattgctcaa
    23761 tcatctttat gacgactacc gcgtagtaac cgaccgcacc attgacagcc acattaaaaa
    23821 tctgcgccgc aagctggaat ctctcgacgc cgaacagtca tttatccgcg ccgtttacgg
    23881 cgttggttac cgctgggaag ccgacgcctg ccgcatcgtt tagttttagc gacattactt
    23941 tgttagccgg agatggcgtt cagacccgat ccggcaacat tattccacgc atggtcagca
    24001 aactgacatt atctctccgc cctggcaggc atcagaaaat cgctcatact ttaatcagta
    24061 aacagcacct ttagatgctg ttttcgataa gcaatttcaa tcaaggagtc attatggctg
    24121 gttggtttga actcagtaag agcagtgata atcagttccg gtttgtgcta aaagcgggca
    24181 atggtgagac tatcctcacc agcgagctgt atacctcaaa agcctctgcg gaaaagggca
    24241 tcgcgtcggt gcgtagcaac agcccgcaag aagagcgcta tgagaaaaaa acggcaagca
    24301 acggcaaatt ctatttcaat ctgaaagccg ctaatcatca aattatcggc tccagccaga
    24361 tgtacgccac cgcgcaatct cgtgaaaccg gaattgcctc cgttaaagcc aatggcacaa
    24421 gccagacggt gaaagacaat acgtaattac gatgccgggc gtgggttgtt ggcgtccggc
    24481 gctttaccag atccccccta cagcgctaca atgcccgccc ttaaagtggg ggcctcccct
    24541 aaccgcttca tcaggtgaag cggatctgac ctgtcatcag aacgagagaa ttatgtttaa
    24601 accggaactc ctttccccgg cgggaacgct gaaaaatatg cgttacgctt tcgcttatgg
    24661 cgcagatgct gtttatgcgg gccagccgcg ttactcccta cgtgtgcgca acaacgaatt
    24721 caaccacgaa aatcttcagc tcggcatcaa tgaagcccac gcgctgggga aaaagtttta
    24781 tgtcgtggtc aacattgcac cgcacaacgc caagctgaaa acctttatcc gtgacctgaa
    24841 accggtggtg gaaatggggc cggatgcgct gattatgtcc gatccagggc tgattatgct
    24901 ggtgcgtgag cacttccctg aaatgccgat ccacctctcg gtgcaggcta acgccgtgaa
    24961 ctgggcgacg gtgaaattct ggcagcaaat gggactgacc cgcgtgatcc tctctcgcga
    25021 gctgtcgctg gaagagattg aagagatcca taatcaggtg ccggatatgg agatcgagat
    25081 cttcgttcac ggcgcactgt gcatggccta ctccggtcgc tgcctgctct ctggctatat
    25141 caacaagcgc gacccgaacc agggcacctg caccaacgcc tgccgctggg agtacaacgt
    25201 ccaggaaggg aaagaagatg acgttggcaa catcgtacac aagtacgagc cgattccggt
    25261 gcaaaatgtt gagccgacgc tgggtatcgg cgcgccaacc gacaaagtgt ttatgatcga
    25321 agaagcccag cgtccgggcg agtatatgac cgcgtttgaa gatgagcacg gcacttacat
    25381 catgaactcg aaagatctgc gcgccatcgc ccatgtagaa cgcctgacca aaatgggcgt
    25441 gcattcgctg aaaatcgaag gtcgtaccaa atctttctac tattgcgcac gcaccgcaca
    25501 ggtttaccgc aaagctatcg atgacgccgc tgcgggaaaa ccgttcgata ccagcctgct
    25561 ggaaactctg gaaggtctgg cgcatcgtgg ctataccgaa ggtttcctgc gtcgtcatac
    25621 tcacgacgat tatcagaact acgaatacgg ttattcagtt tctgaccgcc agcagtttgt
    25681 tggtgagttt accggtgagc gcaaggggga cctcgcagcg gtagcggtga aaaataaatt
    25741 ctccgttggc gacagtcttg agctgatgac gccgcaaggc aacattaact ttacccttga
    25801 gcacatggaa aacgccaaag gcgaagctat gccggtcgca ccaggcgatg gttatactgt
    25861 gtggctcccg gtcccgcagg atcttgagct aaattacgcg ctgctaatgc gtaatttctc
    25921 cggggaaacc acgcgtaacc cccacggtaa gtgattaatt tcgattattt ttcccggatg
    25981 gaaaattctt agaaaccgat cacatacagc tgcatttatt aaggttatca tccgtttcgc
    26041 tgaaaaacat aatccataaa atgctagctg taccaggaac cacctcctta gcctgtgtaa
    26101 tctcccttac acgggcttat tttttgtaca taagctgtaa agaaggattg atccctgata
    26161 accgtcttcc attgaccaca tcgatagaat cccccttcat agcacaatgc ctttcacgta
    26221 acggtatcgt gctcgcccag gttccggcta cgcacagcca gaacgcgcat gtttgacgct
    26281 tacaaaaaaa tattctcact ctccacattt gaatgtcaga cgagcgacgc catgtaatcc
    26341 tgcaccttct gtcttcaggt caactatctg catttttttg ccctgagtaa cacagaaatg
    26401 ggctgcatca ttttttacta tattttctgc accagatatt ctgcccctgg ctaaagaagc
    26461 ttcggcttcg gtgtagtatt ggttatcgag tttacgctga atattacttt tatatgcaag
    26521 accaaattta ccgatacttg tttcatcatt atgcacagca caaccagata taagaaaaat
    26581 actaattaat gatatagcag ctatcttttt catctcacct tcccccatta aataccaacg
    26641 acactctcta gtgtttaaat ataataatgg catgattatt ataattgaat aggattataa
    26701 taaatgttct gtacaacatt tcctacataa gtaggaatta cggacattga ggcccttcag
    26761 ggtaactcca tgggggcttt aatatattat attgaagatg ccactgttta gttgaatatt
    26821 aggtatatgc tcttttttga aatttatcgg tggcagccgt tagtattcgc tgtccccatt
    26881 gcaagctcct ggtggtaacc actgaatcct ccatacttga actgactttt tatcctccga
    26941 ctttcatcct gttctgactc caccttttgt tttctgctct acactatcta cagaccaatc
    27001 ataaaggcac atacgatcat ggcagaattt cccgccagct tactgattct taatggtaaa
    27061 agtactgaca atctaccctt gcgcgaagca attatgctgt tgcgtgagga aggaatgacg
    27121 atccatgtgc gggtcacctg ggagaaaggt gatgccgcac gatttgtaga ggaggcccgg
    27181 aagttgggcg tcgcaacggt gattgccggt ggtggcgatg gcaccattaa tgaagtttct
    27241 acggcgttga ttcagtgtga gggggatgac atacccgcgc tgggaatttt gccattagga
    27301 accgccaatg attttgccac cagtgtaggg attcctgagg cactggataa ggcgctgaaa
    27361 ctggcaattg ccggtaatgc cattgcgata gatatggcgc aggtcaacaa acaaacctgt
    27421 tttattaata tggcgacagg cggatttggg acgcgtatta ccacagaaac gccggaaaaa
    27481 ttaaaagccg cgctgggtgg cgtctcttac atcattcatg gcttaatgcg catggatact
    27541 ctgcaaccgg accgttgtga aatccgcggt gaaaactttc actggcaagg tgacgccctg
    27601 gtcattggta ttggtaacgg gcgtcaggcc ggtggcggtc aacaattgtg cccgaacgcg
    27661 ttaattaacg atggcttgct gcaactgcgc atttttaccg gcgatgaaat tcttccggct
    27721 ctcgtatcaa ccttaaaatc tgacgaagat aacccgaata ttatcgaagg cgcttcgtcg
    27781 tggtttgata tacaagcccc acacgaaatc acttttaatc ttgatggcga accgttgagt
    27841 gggcaaaact tccatattga aatacttccg gcggcgttgc gttgtcgatt accaccagat
    27901 tgtccattat tgcgttaatc aaactattct ctcttgaacc agggagaatc cgtaatgccg
    27961 gatgcggtgt aaacacctta tccggcatac agaacaatac tacaccgtaa ttaattttac
    28021 ccgtgtcttc tgcaatgcca atttatccac ctctgacaca gcatcatcgg taacaataat
    28081 gtcaattttt tcgattggca gtacctgatt aaaaccgcga cgattaaatt tcgatgaatc
    28141 gagaacggca atcactttat ttgcggcggt gaccatcgcc ccgctaatcg aataaccttc
    28201 attaaaggtc gtaataccat taaccgcatc aataccatca gcaccgacaa acattaaatc
    28261 ggcattaata tcctgcaatg aacgctcggc aatggaacca tgcatcgagc gcgttttatg
    28321 gcggaccgtg ccaccacaga cgaccagagt aatgtcttta ttttcggaaa gggcaaacgc
    28381 tgccgggaga ctgttggtaa tcaccgtgat atttttagcg gtcattaatc cttcggcgat
    28441 aagcattgtg gtacttccgc tgtcgagaat aacggtcatc ccttcatgga tcattttgac
    28501 tgccgcctgc gctattctgt tttttggcgc gctggcgagc tgaaatcgct ctttaaaccc
    28561 gacttcctgg gtctcggttt cactattacc ggacattatt ttcgccgcac cgccatgaaa
    28621 gcgcgtaacc acgccttttt gttcgagaaa gcgcaaatcg gcacggattg tcgcttccga
    28681 ggcagcaaat actcccgcca gatcctgaac aagcacggtt ccctgttcat tcactaattg
    28741 gatgatctta ttccttcgct cgaatgaatt catcgtcatt tacccatatt caattgtggc
    28801 tagtgtaaac gaagggggac cattggtgaa tgatctgttt tgcacaatac cgggccagca
    28861 tcacgctggc ccgcggtttc atggaatgag caacactttg ccctgcatgg cattacgagc
    28921 gatgtcacgc accgcctggg tgaagctttc aaagcttcca cggtgagcga ttaatggctc
    28981 caggcttaac ttacgttctg tcagcaaccg gctcgccgtt tcccactcct gccccggcca
    29041 agggctggaa tagttcatcc aactgccgat aaccgtcagc tctttacgca atattttgcc
    29101 aaacgttgcc gatgttaaat gcagatcctg atgcaacgtg cccaccagcg ccagttgggc
    29161 atgaggaccg gcaatctcta ccgccagttc gacagtttgc ggtacgccag ccgtctcgag
    29221 gataagctga ttaaagcgca gttcgcgtaa aacgctctgc atttgcggcg cgctcatttc
    29281 gctactgtta aatgtttgca tcgcaccgaa agattttgcc agtgccagtt tttctgagct
    29341 aatgtcgatc gccgtcacac tctttgctcc cagcgcgaca gcgcactgaa tagccagcag
    29401 gccaatggtt ccggcaccaa taataataac gtttttattc tcacaacctt gcgctaaatg
    29461 aaaagcatgc aggccaacgg taatcggctc aataaaagcc ccatcctcaa taggcatatc
    29521 cgtgggtaga gcaaagacat tttttcgctt aacgacaata tattcagcaa atccaccatc
    29581 acgccgcgag ccaataaaat catattttgc gcactgggaa taaaaccctt tcaaacactc
    29641 tggacaagta aaacagggta ataacggcac acaggcaacc gcatcgccag ggtgtaaatc
    29701 atcaacaccg gatcccaccg catcaatata gccgctaaat tcatggccta acgttattgg
    29761 ataataatgt gcaccatttt taaatatcct gggtaaatcg gaaccacata agcccgagct
    29821 ggcaattttt acccgcacct catcctgatg tttaatttca ggaatgacgc tttttgcaac
    29881 gcgcacgata ccatcagtat cattcactac tgatttcata aaaactcctg att
//
LOCUS       DAJTUI010000043        29466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTUI010000043 DAJTUI010000000
VERSION     DAJTUI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.043"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..213)
                     /locus_tag="OO464_002078"
     CDS             complement(<1..213)
                     /locus_tag="OO464_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR6227229.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="OO464_002079"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="OO464_002079"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6227230.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="OO464_002080"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="OO464_002080"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6227231.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="OO464_002081"
     CDS             2894..3634
                     /locus_tag="OO464_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6227232.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="OO464_002082"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="OO464_002082"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6227233.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4192..4794)
                     /locus_tag="OO464_002083"
     CDS             complement(4192..4794)
                     /locus_tag="OO464_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR6227234.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4864..4929
                     /gene="yobI"
                     /locus_tag="OO464_002084"
     CDS             4864..4929
                     /gene="yobI"
                     /locus_tag="OO464_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR6227235.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5068..5679
                     /gene="ruvA"
                     /locus_tag="OO464_002085"
     CDS             5068..5679
                     /gene="ruvA"
                     /locus_tag="OO464_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6227236.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5688..6698
                     /gene="ruvB"
                     /locus_tag="OO464_002086"
     CDS             5688..6698
                     /gene="ruvB"
                     /locus_tag="OO464_002086"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6227237.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="OO464_002087"
     CDS             complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="OO464_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6227238.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="OO464_002088"
     CDS             complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="OO464_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6227239.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8461..9393
                     /gene="znuA"
                     /locus_tag="OO464_002089"
     CDS             8461..9393
                     /gene="znuA"
                     /locus_tag="OO464_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6227240.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9409..10731
                     /gene="mepM"
                     /locus_tag="OO464_002090"
     CDS             9409..10731
                     /gene="mepM"
                     /locus_tag="OO464_002090"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6227241.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10851..11822
                     /gene="lpxM"
                     /locus_tag="OO464_002091"
                     /gene_synonym="msbB"
     CDS             10851..11822
                     /gene="lpxM"
                     /locus_tag="OO464_002091"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6227242.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="OO464_002092"
     CDS             complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="OO464_002092"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6227243.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="OO464_002093"
                     /gene_synonym="yebK"
     CDS             complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="OO464_002093"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6227244.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14729..16204
                     /gene="zwf"
                     /locus_tag="OO464_002094"
     CDS             14729..16204
                     /gene="zwf"
                     /locus_tag="OO464_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6227245.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16439..18250
                     /gene="edd"
                     /locus_tag="OO464_002095"
     CDS             16439..18250
                     /gene="edd"
                     /locus_tag="OO464_002095"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6227246.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18287..18928
                     /gene="kdgA"
                     /locus_tag="OO464_002096"
                     /gene_synonym="eda"
     CDS             18287..18928
                     /gene="kdgA"
                     /locus_tag="OO464_002096"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6227247.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18984..20162)
                     /gene="purT"
                     /locus_tag="OO464_002097"
     CDS             complement(18984..20162)
                     /gene="purT"
                     /locus_tag="OO464_002097"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6227248.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20296..20586
                     /gene="yebG"
                     /locus_tag="OO464_002098"
                     /pseudo
     CDS             20296..20586
                     /gene="yebG"
                     /locus_tag="OO464_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20653..21009
                     /locus_tag="OO464_002099"
     CDS             20653..21009
                     /locus_tag="OO464_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6227249.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21336..21995
                     /locus_tag="OO464_002100"
     CDS             21336..21995
                     /locus_tag="OO464_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6227250.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22205..24265
                     /gene="ptrB"
                     /locus_tag="OO464_002101"
     CDS             22205..24265
                     /gene="ptrB"
                     /locus_tag="OO464_002101"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR6227251.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="OO464_002102"
     CDS             complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="OO464_002102"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6227252.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24948..25604)
                     /locus_tag="OO464_002103"
                     /pseudo
     CDS             complement(24948..25604)
                     /locus_tag="OO464_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(25706..25936)
                     /gene="holE"
                     /locus_tag="OO464_002104"
     CDS             complement(25706..25936)
                     /gene="holE"
                     /locus_tag="OO464_002104"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6227253.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26075..26449
                     /gene="yobA"
                     /locus_tag="OO464_002105"
     CDS             26075..26449
                     /gene="yobA"
                     /locus_tag="OO464_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6227254.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26453..27325
                     /gene="copD"
                     /locus_tag="OO464_002106"
                     /pseudo
     CDS             26453..27325
                     /gene="copD"
                     /locus_tag="OO464_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            27338..27679
                     /locus_tag="OO464_002107"
     CDS             27338..27679
                     /locus_tag="OO464_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR6227255.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28072..29149)
                     /locus_tag="OO464_002108"
                     /pseudo
     CDS             complement(28072..29149)
                     /locus_tag="OO464_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(29115..29396)
                     /locus_tag="OO464_002109"
     CDS             complement(29115..29396)
                     /locus_tag="OO464_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR6227256.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         7437 a         7235 c         7568 g         7226 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcaa cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cagcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
     4441 acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga
     4501 gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt
     4561 tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga
     4621 aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc
     4681 gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat
     4741 acatgtaaag acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc
     4801 attccattga aatagataca aatatacgta atagcataaa aaaattattt ttcaggagtc
     4861 gcaatgtata tttttattac gcatttcttc actgaatatg taatattcaa atatttgctt
     4921 ccaatataac ctgttgaata aattatactg tgccattttt cagatcatcg ggacacctcg
     4981 caagttttct tcatccttcg ctggatatct atccagcatt tttttatcat acagcattat
     5041 ctttgattca ttacgcagga gcgtcatgtg ataggcagac tcagaggcat catcattgaa
     5101 aaacaacccc cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt gcatatgccg
     5161 atgacctgtt tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt
     5221 gtggtgcgtg aagacgcgca actgctgtac ggttttaaca ataaacaaga acgcacgttg
     5281 ttcaaagagt tgatcaaaac caacggcgtc ggcccgaagt tggccctggc gatcctctcc
     5341 ggaatgtcag cgcagcagtt cgttaatgcc gttgagcgtg aagaagtggg ggcactggtg
     5401 aaactgccgg gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat gaaagaccga
     5461 tttaaaggtt tgcatggcga tctctttacg ccagccgccg acctggtact cacgtctcct
     5521 gctagcccgg cgaccgacga tgctgaacaa gaagcagttg ccgcgctggt ggcgctaggc
     5581 tataaaccac aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga caccagcagt
     5641 gaaacattaa ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg attgaagcag
     5701 accgtctgat ttctgccggt accactttgc cggaagatgt agcagatcgc gccattcgcc
     5761 ctaaattact ggaagagtat gttggtcagc cgcaggttcg ttcacagatg gagattttca
     5821 tcaaagcagc gaaactgcgc ggcgatgccc tcgatcattt gttgattttt ggtcctccgg
     5881 ggttgggtaa aactacgctt gccaacattg tcgccaatga aatgggcgtt aatttacgca
     5941 cgacttctgg tccggtgctg gaaaaggcgg gcgatttggc tgcgatgctc actaaccttg
     6001 aaccgcatga cgtgctgttt attgatgaga tccaccgttt atcgccagtt gttgaagaag
     6061 tgctgtaccc ggcaatggaa gactaccaac tggatatcat gattggtgaa ggtccggcgg
     6121 cacgctccat taaaattgat ttgccgccgt ttaccctgat tggcgcaacc acgcgcgcag
     6181 gttcgctgac atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc
     6241 aggtgccgga tctgcaatat atcgtcagtc gcagtgcacg ctttatgggg cttgagatga
     6301 gtgatgacgg tgcgcaggaa gtagctcgtc gcgctcgcgg tacgccacgt attgccaacc
     6361 gtctgctgcg tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc atctcggcag
     6421 atatcgctgc tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc gattatatgg
     6481 accgcaaatt gttgctggcg gtaatcgata agttctttgg tgggccggta ggtctggata
     6541 acctggcggc agccattggc gaagaacgtg aaaccattga agatgtactg gaaccttatt
     6601 tgattcagca aggctttttg cagcgtacac cgcgcgggcg tatggcgacg acgcgggcgt
     6661 ggaatcactt tggcataacg ccgccagaaa tgccgtaagt cggattggtc gctcgcgccg
     6721 catccgacac tggcagtgtg tctgatgcga cactgttcgc gtcttatcag gccagccaga
     6781 gatagcgaac cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga
     6841 tagattagct ggcctgcttt ttcatcatac tgataataaa taacagtgcc gcacatagca
     6901 ccaccgacgg acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg
     6961 ccaccatccc caccaaaaca gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac
     7021 gcgcagtagc tgcaggaata atcagcaacg aagtaataat caacgcaccg acaaatttca
     7081 tcgctacacc aatcgtcaat gccgtcacca gcatcaacaa caatttcact tgctgtaatt
     7141 tcacaccatc aacaaacgcc agatccgggc taatcgtcat cgacagcaaa ttgcgccatt
     7201 gccagaacaa aatagccacc acgatgacca cgccaatcgc aatagagatg agatcttctg
     7261 gcgtcactgc cagtaaatca ccgaacaggt aagccatcaa atcaacacga atgttagaca
     7321 tcagactaac gaccactagg cccagcgaca gggcactgtg cgccataatc cctaataacg
     7381 tgtcgatcgc cagctgtgga cgcttctcca gccataccag accgcccgcc agcagcagcg
     7441 taacggcaat caccgcatag aatggattca cgtccagcaa caaaccaaac gcgacaccaa
     7501 gtaatgaggc atgagccagc gtatcaccga aataagacat acgacgccag actacaaacg
     7561 aacccagcgg acccgcggca caggcgagca tgatcccggc taaccaaccg ggaaataata
     7621 attcaatcat gagcgatcat ttccccgacg caaaacaatt cgtccctgta aatcgtgacg
     7681 atgattatga tgatggcgat agatacccag ttgttcagca ccacgaggac caaacattga
     7741 aataaactcc ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt
     7801 caggcacagc acttcatcgg tttttgccat caccagatgc agatcgtgag aaaccattaa
     7861 aacgccacaa tccagttcac gacgcaactg gtcaataagg tcatataacg ccacttggcc
     7921 atttacatcc acgccttgag tgggttcatc cagcactaat aattgcgggc aatttaacaa
     7981 tgctcgcgct aatagtacac gctgcgtttc gccaccagag agcttttgca tcggtgcgtt
     8041 aatcaggtgc ccggcctgga cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc
     8101 agggcgtaag cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga gatacagctt
     8161 ctgcggtaca tagccgatgc gcagttttcc gttgcgcttg ataacccctt catcgggtgt
     8221 taccagcccg agcactaccc gtaccagtgt cgacttacct gcgccatttg gcccaagtaa
     8281 agtcaaaatt tttccaggtt taagttccag cgacacatca gagaggacgc ggcgttggcc
     8341 aaaagaaacc gagacatttt ccagggaaac cagacttgtc atgttaattt tagtcttgca
     8401 gtagtcatga aatgttataa tatcacactt ctcatattca ttacgatgat tggtcgcatt
     8461 atgttacata aaaaaacgct tcttttcgca gcattatccg ccgctctctg gggaggtgca
     8521 acacaggccg cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct
     8581 gccattgctg atggtgtaac agaaacggag gttttactgc ctgacggcgc ttcagaacat
     8641 gattattcac tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg
     8701 gttggcccgg agatggaagc gtttatgcaa aaaccggtaa gcaaattacc agaagcgaag
     8761 caggtaacga ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag tattcacggc
     8821 gatgatgatg atcacgacca cgcggaaaaa agtgacgaag atcaccatca cggcgatttc
     8881 aacatgcatc tttggctttc cccagagata gcgcgggcta cagcggttgc aatccatgga
     8941 aaattagtgg aacttatgcc gcaaagtcga gccaaacttg acgccaacct gaaggatttt
     9001 gaggcacaat tggcctcaac cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg
     9061 aaaggttatt tcgtttttca cgatgcttac ggctatttcg aaaaacagtt cggactgaca
     9121 ccgcttggtc attttaccgt taaccctgag attcaacctg gcgcgcagcg tttacatgaa
     9181 ataagaacac agttggttga gcaaaaagca acctgcgttt ttgctgagcc acagttcagg
     9241 ccagcggtcg ttgagagcgt cgcacgaggg acatccgttc gtatgggaac gttggatccc
     9301 ctggggacga atatcaaact gggtaaaaca agctattcag aattcctgag tcaattagcc
     9361 aaccagtatg cgagctgcct gaaaggagat taatgaggaa gtgattacgt gcaacagata
     9421 gcccgctctg tcgccctggc gtttaataat ttaccgcgac cacaccgcgt tatgttgggg
     9481 tcgctcaccg ttcttactct ggccgtcgct gtctggcggc cctatgttta tcaccgtgat
     9541 gccacgccaa ttgtcaaaac cattgagctg gaacagaacg aaattcgttc gctcttacct
     9601 gaagccagtg agccgattga tcaagctgca caagaagatg aagccattcc ccaggacgaa
     9661 ctggatgaca aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc cactggcgat
     9721 acgctaagca gcattctcaa tcagtatggt attgatatgg gtgatatcac ccaacttgct
     9781 gctgccgaca aagaattgcg taacctgaaa atcggtcaac aactctcctg gacattaacc
     9841 gcggacggcg aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat
     9901 gaccgtactg ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt
     9961 aacaatctgc tgaaaggtac cgtcggaggg agctttgttg ccagcgccag aaacgccggt
    10021 ttaaccagcg ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat ggatttccgc
    10081 aaactgaaaa aaggcgatga atttgcggta ttaatgtctc gagaaatgct tgatggtaaa
    10141 cgtgagcaaa gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga ttattacgca
    10201 atccgcgctg aggatggcaa attctacgat cgtaacggta ctggtctggc gaaaggattc
    10261 ttgcgattcc cgacggcgaa acagttccgt atctcgtcta actttaaccc gcgtcgaact
    10321 aatccggtga caggtcgcgt tgcaccacac agaggtgttg atttcgccat gccacaaggt
    10381 acgccagtgc tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca
    10441 ggttattatg tggctattcg tcatggtcgc agctacacca cgcgttatat gcacttgcgc
    10501 aagattctgg tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc gctttccggt
    10561 aataccggac gttcaactgg gccgcatctg cactatgaag tatggataaa ccagcaggcc
    10621 gtaaacccgc tgacggcaaa actgccgcgt accgaagggc tgaccggctc cgatcgtcgc
    10681 gaattcctgg cgcaggccaa agagattgtg ccgcagctac ggtttgatta attaacatcc
    10741 attcgcagcc ggtacgcagt cagtaccggc tttttttatt tggtgcgggg caagttgtgc
    10801 cgctacacta tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc atggaaacga
    10861 aaaaaaataa tagcgaatac attcctgagt ttgataaatc ctttcgccac ccgcgctact
    10921 ggggagcatg gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc
    10981 gtgatcccat tctggcacgg ctgggacgtt ttgccggacg actgggaaaa agctcacgcc
    11041 gtcgtgcgtt aatcaatctg tcgctctgct ttccagaacg tagtgaagct gaacgcgaag
    11101 cgattgttga tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg
    11161 caatacgcgg gccggagaaa attcagccgc gcgttggctg gcaagggctg gagatcatcg
    11221 aagagatgcg gcgtaataac gagaaagtta tctttctggt gccgcacggt tgggccgtcg
    11281 atattcctgc catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc
    11341 agggcaaccc ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc
    11401 tgcatgcgag aaatgacggt attaaaccat tcatccagtc ggtacgtcag gggtactggg
    11461 gatattattt acccgatcag gatcatggcc cagagcacag cgaatttgtg gatttctttg
    11521 ccacctataa agcgacgttg cccgcgattg gtcgtttgat gaaagtatgc cgtgcgcgcg
    11581 ttgtaccgct gtttccgatt tatgatggca agacgcatcg tctgacgatt caggtgcgcc
    11641 caccgatgga tgatctgtta gaggcggatg atcatacgat tgagcggcgg atgaatgaag
    11701 aagtcgagat ttttgttggt ccgcgaccag aacaatacac ctggatacta aaattgctga
    11761 aaactcgcaa accgggcgaa atccagccgt ataagcgcaa agatctttat cccatcaaat
    11821 aaaaaagcct ctcgcgagga gaggccttcg cctgatgata agttcaagtt tgcttcagaa
    11881 tattcgaaat ctgttgaact atcattgaac tataggccgg atgtggcgtt ttcgccgcat
    11941 ccggcaacgt acttactcta ccgttaaaat acgcgtggta ttagtagaac ccacggtact
    12001 catcacgtcg ccttgggtga caatcaccag gtcaccagac atcaagtaac ctttatcgcg
    12061 cagcagatta accgcttcgc tggcagctgc tacgccgtca ttagcgctat caaagggcac
    12121 cggcgtaacg ccacgataga gagcagtcag gttcagcgta cgttcatggc gcgacatggc
    12181 gaaaattggt agaccagagc tgatacggga ggtcatcagc gcggtacgac ccgattcggt
    12241 catggtaatg atcgccgtaa cgcccttcag gtggttagct gcgtacattg ccgacatggc
    12301 aatagcttct tccacattgt cgaactgaac gtccagacgg tgtttagaaa cgttgatgct
    12361 cgggattttt tccgcaccca ggcaaacgcg cgccatggct gcaacagttt ctgacggata
    12421 ctgcccagcg gcagtttctg cagacagcat cacagcgtca gtaccatcca gaacggcgtt
    12481 tgctacgtcc atgacttctg cacgcgtcgg catcgggtta gtaatcattg actccatcat
    12541 ctgggtcgcc gtgattaccg ctcggtttag ctgacgcgca cgacggatca acgctttctg
    12601 aatgccgacc agttccgggt cgccaatttc cacaccgagg tcgccacgtg caaccattac
    12661 cacgtcagag gcgaggatga tgtcatccat tgcatcctgg ctgcaaacgg cttccgcacg
    12721 ttcaaccttg gcaacaattt tcgcatcaca tcctgcatcg cgtgccaggc gacgggcata
    12781 gttcagatct tcgccacagc gtgggaagga gacagccagg taatctacgc caatcaacgc
    12841 cgcagtctta atgtctgctt tgtctttttc ggtcagcgct tcagccgaca aaccgccgcc
    12901 aagtttgttg atacctttat tgttggagag gggaccaccg acggtgactt cggtgaacac
    12961 tttcatgccc tgaacttcca gtacttttaa ctggacgcga ccatcgtcca gcagcaggat
    13021 gtcaccaggc acgacgtcag taggcaggcc tttgtagtcg ataccgactt tttctttgtc
    13081 gccttcacct ttacccaggt tggcgtcgag caggaattta tccccaatat tgaggaaaac
    13141 tttgccttct ttaaaggtgg atacacggat tttgggcccc tggaggtcac ccagaatagc
    13201 cacatgacgc cccagttttg cggcaatctc acgaacttta tccgcgcgca ttttgtgatc
    13261 ttcaggcgag ccgtgagaaa agttcatacg tacaacgttg gcacccgccg cgataacttt
    13321 ttcaagatta ttatcgcgat ctgttgctgg gcctaacgtg gtaacgattt ttgttctgcg
    13381 aagccttttg gacatgtaat actccgttga ctgaaacaac caggtgttgc ttgaacatga
    13441 aatccgaatg aaataacgcc gcgatggcga taaattaatc tcattcggat gtcgtataag
    13501 attaggacag tgacagtcgc ttttagcgat cgtcacttaa attaagtaac tgcttatcaa
    13561 aacgcgattc cttcagcgct tccttgaccc gcttcaagtt atctctgaat tttgcaccgc
    13621 gtcgcaaagt aaatcctgtc gccagcacat ctatcacggt cagctgtgca agtcgagaaa
    13681 ccatgggcat ataaatgtca gtatcttccg gtacgtcgag ggtaattgcc agcgttgctt
    13741 cccgggcgag cggggtaccc gcagaggtga gggcaatcac catggcgtcg ttttcgcgtg
    13801 ccagctgcgc cagctcgacc agattttttg ttcttcctgt gtgagaaatc agcaccacca
    13861 cgtctccgtc gctacaattc atacaactca tgcgttgcag cacgatatca tcggagtaca
    13921 ccaccggaac attaaaacga aagaacttat tcatcgcatc gtgggcaacg gcggcagaag
    13981 agcctaatcc gaaaaaggcg attttttttg cctgagtgag caagtcgacg gcgcggttga
    14041 tggcagattt atccagtgaa tgacggacat gatcgagcgt tgccattgcg gactcaaata
    14101 ttttccccgt gtatgattca acgctgtcat cttcattgac gttgcgatta acatagggag
    14161 tgccattcgc cagactttgt gccagatgaa gtttaaaatc aggaaaaccg cgcgtgtcca
    14221 tgctgcgaca gaaacgattc accgtcggtt cgctaacatt ggcttccagc gccagagcag
    14281 caatactcga atggatcgcg ttatcgggcg aagccagaat gacctcggca actttgcgct
    14341 ctgatttgct caaatgttcc agctgagact ggattttttc cagcatattc atgatgtaaa
    14401 gagactcacg ggtaatgacg atttccgcac tgaaagaaat cgaaatgcag ttttgtcaga
    14461 tattacgcct gtgtgccgtg ttaatgacaa aagcagataa aaaagttgtt atttttttca
    14521 taacatgatc agtgtcagat ttttaccgaa tggaaaacga tgattttttt atcagttttg
    14581 ccgcgctttg cgcgcttttc ccgtaatcgc acgggtggat aagcgtttac agttttcgca
    14641 agctcgtaaa agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta
    14701 ccgggttagt taacttaagg agaatgacat ggcggtaacg caaacagccc aggcctgtga
    14761 cctggtcatt ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta
    14821 tcaactggaa aaagtcggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc
    14881 tgactgggat aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa
    14941 agaaaccatt gatgaaggtt tatgggacac cctgagtgca cgtctggatt tttgtaatct
    15001 cgatgtcaat gacactgctg cattcaaccg tctcggcgcg atgctggatc aaaaaaatcg
    15061 tatcactatt aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct
    15121 tggcgaggca aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac
    15181 gtcgctggcg acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca
    15241 ggtttaccgt atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg
    15301 ttttgctaac tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat
    15361 taccgtggca gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca
    15421 gatgcgtgac atgattcaga accacctgct acaaattctc tgcatgattg cgatgtctcc
    15481 gccatctgac ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtccct
    15541 gcgccgcatc gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg
    15601 cttcgcccag ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaaaagcag
    15661 caatacagaa accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt
    15721 gccattctac ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta
    15781 tttcaaaaca cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa
    15841 actgactatc cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc
    15901 tggccttgac cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac
    15961 ctttaatcag acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg
    16021 tattcaggca ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat gggtagactc
    16081 cattactgag gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg
    16141 gggacccgtt gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga
    16201 gtaatatcag cgcttatcct ttatggttat tttaccggta acatgatctt gcacagattg
    16261 tagaacaatt tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta
    16321 cactgactga aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc
    16381 tctgaatgaa acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat
    16441 gaatccacaa ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc
    16501 tgcttatctc gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc
    16561 atgcggtaac ctggcacacg gtttcgctgc ctgccagcca gaaaacaaag cctctttgaa
    16621 aagcatgttg cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgcgca
    16681 ccagccttat gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt
    16741 tggtcaggtt gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg
    16801 aatggaattg tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg tggggctgtc
    16861 ccataacatg tttgatggtg ctctgttcct cggtgtgtgc gacaagattg tcccgggtct
    16921 gacgatggca gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat
    16981 ggcaagcggt ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa
    17041 agtggaccgc atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg
    17101 tactttctac ggtactgcca acaccaacca gatggtggtg gaatttatgg ggatgcagtt
    17161 gccaggctct tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc
    17221 tgcgcgtcag gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat
    17281 gatcgatgag aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac
    17341 taaccacacc atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga
    17401 tgacttctct gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc
    17461 ggccgatatt aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct
    17521 caaagcaggc ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta
    17581 tacccttgaa ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc
    17641 actcgacagc aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa
    17701 agtgttaagc ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa
    17761 ccaggtgatt gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt
    17821 tgaagcgggt ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc
    17881 gaacggaatg ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg
    17941 tttcaaaatt gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc
    18001 agctatccac gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg
    18061 ggacatcatt cgtgtgaatg gacagacagg cgaactgaca ctgctggtag acgaagcgga
    18121 actggctgct cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg
    18181 tgaattattc agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat
    18241 cactttttaa gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa
    18301 aacaagtgca gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa
    18361 actggaacac gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga
    18421 agtgactctg cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc
    18481 tgaagcgatt gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga
    18541 agcgggtgca cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac
    18601 cgaagggact attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat
    18661 ggactacggt ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc
    18721 cctgcaggcg atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc
    18781 tccggctaac taccgtgact acctggcact gaaaagcgtg ctgtgcatcg gtggttcctg
    18841 gctggttccg gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg
    18901 tgaagctgta gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg atcgggcatt
    18961 tttgtagcgt ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc
    19021 gcgcgttcaa tggcgtcaac aacactctct gaagtagcca gtaccacccc cagatgacgg
    19081 ctgccatcaa tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc
    19141 tgcacattat caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct
    19201 gcaggaccat actgacggat cccgccaaca ggaagtccga ggaaggcacg tacatgcagg
    19261 gcaaactctg agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg
    19321 gagacctcac tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca
    19381 taaccgccca gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc
    19441 gggctcattt gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct
    19501 actggtgcac agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag
    19561 tcaaacttaa cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga
    19621 gcgtattccc atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg
    19681 gaagagctca tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg
    19741 aaaaggcttt tgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc
    19801 gccaggcgac ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca
    19861 acattcagtc cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc
    19921 ggcacgatat aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc
    19981 atattaatga catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg
    20041 acggcaatca cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca
    20101 ccggagccta ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc
    20161 ataacgaata tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt tgcgtgtgtc
    20221 tttattgctg atgttgattt ctcaaccgaa aagaaatata ctgtataaaa tctcagttat
    20281 tatgagaggc tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag
    20341 aaaatgtcgt ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg
    20401 gatctgcttg acacctagct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa
    20461 gccctttcgc tatggctggc agaacaaaaa gaggtgctga gcaccattct gaaaaccggc
    20521 aaattaccgt ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg
    20581 gcctgaagca acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct
    20641 atggagaaaa acatgaaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc
    20701 gcatcagttt tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa
    20761 ggtctggatg ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg
    20821 gcgcgctggg cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt
    20881 ttgcaggaca ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa
    20941 agtgccgata ttcattacca ggtctgcgtg gactgcaaag cgggaatggc ggaatatcag
    21001 cggcgttaat ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg
    21061 tccgtcccag aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc
    21121 aatcagcatc aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat
    21181 cacctgatat acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc
    21241 cccgcttatt tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg
    21301 ctacaggcac taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc
    21361 aatccctgct tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca
    21421 aactgttagt gtcaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag
    21481 cacgtaaact tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag
    21541 cgggtacggt gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac
    21601 cgcagtttgg cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc
    21661 tggtctttgc cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc
    21721 agcaattgcg tgaagccggc gtggaagagc aggggcgtgt actcattgag caggcaatcg
    21781 aacaaccgct ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg
    21841 aaatctattt cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc
    21901 tgaatgcact gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg
    21961 atctcgaaca gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt
    22021 tgactatttc gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct
    22081 gcataacatt cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt
    22141 cgcttgcatc cccggtgagt tttgccaccc ttataagatg tttccaacca gaaagaacaa
    22201 taacatgcta ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg
    22261 catcgataat tactactggc tgcgggacga tacgcgttct cagccaaaag tcctggacta
    22321 cctgcaacaa gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga
    22381 tcgcatctta aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat
    22441 aaaaaatggc taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca
    22501 gcgtcaatcg gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa
    22561 gcgcgcagct catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac
    22621 cattatggcg ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa
    22681 tctggaaaca ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg
    22741 ggcaaatgac tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta
    22801 tcaggtctgg cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga
    22861 aaaagacgat acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat
    22921 tcatctggcc agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc
    22981 cgagccgttt gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca
    23041 gcatcgtttt tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg
    23101 tatgcgtgat gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga
    23161 agggtttacg ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag
    23221 tttgcgccaa attaaccgca agacccggga agtcattggc attgcctttg atgatccggc
    23281 ctatgtgacc tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta
    23341 ttcttccatg accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg
    23401 tgtattaaaa caaacagaag tgcctggttt tgatgcggcg aattaccgca gtgaacacct
    23461 gtggatagtc gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca
    23521 ttttcgcaaa ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat
    23581 tgatgccgat ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat
    23641 tgtccatgtt cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct
    23701 gaagaagaaa aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg
    23761 ctatggctct ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg
    23821 cgttgcaatt aatcaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt
    23881 tgatgttgta acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga
    23941 gtggggaaat ccgcaggatc cgcaatatta cgagtacatg aaaagctaca gcccgtatga
    24001 caacgtcacc gcacaggctt atccgcattt actggtaacg accggtttac acgattctca
    24061 ggtgcaatat tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga
    24121 ccatctttta ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt
    24181 taaatcgtac gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac
    24241 attacccgct acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag
    24301 ctccgggctc atgctgtcca ggttattaaa taaccagcgc agatagcccg gatagcgttc
    24361 ggcaacgtcg gaaaccgctt tgccacggta tttgccaaag gtgaaggttg tcatcaacga
    24421 cggacgtccg gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc
    24481 gataagcaac gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccag
    24541 cggtgtctgt acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc
    24601 tggccacaaa cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag
    24661 tacgcggcgg tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg
    24721 gatcacatct tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc
    24781 catcgcttgt ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat
    24841 ttttccgtca atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt
    24901 ttctgtatcg ataatgcgca acatggcatg atccaggccg aaaaagccta gcgtaatgga
    24961 atgatatcgc cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga
    25021 tcggcgcgaa cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt
    25081 gccatcagca cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc
    25141 agcaggtcgg gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata
    25201 tccatacctt gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat
    25261 gcaccatgac tctgatggta gttcccaggc gtttttcgcc acggtgcgaa caccgcaatt
    25321 ccacgaataa agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgctag
    25381 gtcgttgcgg cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca
    25441 cgtcgcgaat aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt
    25501 tgcctggcgg cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt
    25561 ttacgaggct catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca
    25621 aaatgactgc aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt
    25681 aagaaagtgt aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca
    25741 gattgaccga agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt
    25801 ctggctgttc acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag
    25861 ccaccccggc cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag
    25921 ccagattctt cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga
    25981 gcctggatgg caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc
    26041 tgtaaaaaag ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg
    26101 ctacgcgctg gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca
    26161 tttaacgcat cagtatcctg cggcaaacgc gcaagtgaca gctgccccgc aggcaatcac
    26221 tttaaacttc tcggaaggcg ttgaaacagg attcagtggt gcaaaaatca cggggccaaa
    26281 aaacgaaaat attaaaacat taccagcgaa gcggaatgag caggaccaaa agcaactgat
    26341 tgttccactg gccgattcgc tgaaacccgg aacctacacc gttgactggc atgttgtttc
    26401 ggtggacggg cataaaacga aaggacacta cacctttagc gtgaaataaa tcatgctggc
    26461 gtttacctgg atcgcgctgc gatttatcca tttcacctcg ttgatgctgg tctttggctt
    26521 tgcaatgtat ggtgcctggc tggccccctt aatgattcgt cgcttgctga cgaagcgttc
    26581 tctgcgttta caacagcacg ctgccgtctg gagtttgatc agtgccactg caatgctcgc
    26641 cgttcagggc gggttgatgg gaacgggatg gacagatgta ttttcgccaa acatttggca
    26701 ggctgttttg caaacgcagt ttggtggcgt ctggttatgg caaattgttc tcgccctcgt
    26761 aacgttgatc gtcgccctta tgcaaccgcg gaatatgcca cgtttgctgt ttatgctcac
    26821 taccgcgcaa tttattctgc tggcgggagt ggggtatgcg acgctgaatg aaggggtaac
    26881 agcgaaaatc catcagacta atcacgcgat tcacctgatt tgtgcggcgg cgtggtttgg
    26941 gggattactg ccggtgctct ggtgtatgca actcatcaaa ggccgttggc gacactaggc
    27001 tattcaggcg ctgatgcgtt tttcctggtg cgggcatttt gcggtgatcg gcgtactggc
    27061 aagcggcgtg cttaatgcat tgttgattac cggatttccc cccacgctta cgacttactg
    27121 gggccaactc ttattgctca aagccatcct ggtgatgatt atggtggtca ttgcgctggc
    27181 taatcgttat gttcttgtac cgcgcatgag gcaggatgaa gatcgtgcgg caccctggtt
    27241 tgtgtggatg actaaactcg aatgggcgat aggtgcggta gtgctggtga tcatcagcct
    27301 gcttgcgact ctcgaacctt tttgatggat tacgaaaatg atgaaaaaaa gtattctggc
    27361 gtttctgtta ctcaccagtt ctgcagcggc gctggcggct ccacaggtga taaccgtgag
    27421 tcgttttgaa gtgggtaaag acaaatgggc gtttaatcgc gaagaggtaa tgctgacttg
    27481 ccgaccgggt aatgctttgt atgtcatcaa cccaagtacc ctcgtgcagt atcctttaaa
    27541 cgatatcgca caaaaggaag ttgccagtgg gaagaccaat gcccaaccca tttcggtgat
    27601 tcagattgat gatcctaaca atcccggcga aaaaatgagt ctggcaccgt ttatagaacg
    27661 agctgaaaaa ctctgttaat tacctaaaat agccttttga tttccaataa aaaaaccgcc
    27721 tcagttcttt caccagaacg ggcggttttt aacatttaag ctgatgagca ccgcgctttt
    27781 tattgaccat tttgcacgca aactggaaaa cctggcgtcg tcatctattc ttaaagggca
    27841 aggcaactaa gcctgcatta atgccaactt ttagcgcacg gctctctccc aagagccatt
    27901 tccctggacc gaatacagga atcgtattcg gtcttttttt aattgtattt aaaatcaatt
    27961 agttgcaaac gtctccccga aattccccga aatttactcg aatttctgta ttccggtctt
    28021 ttttggttat atcacaacca aaatacattt aacaatccat ttacgttaaa atcagagcag
    28081 taagtacgtt ttttctctct catcaagata catttttgtt gtcttctccg atgtgtggcc
    28141 aagtagacgc tgagcaaatt cttctccaca tatttctttg tacaatcgtc cagccaggct
    28201 tctgatttcg tgaaaagttg gtgggttttc actgaacagt gatacctgtt aattttctgg
    28261 ctgcgacaaa tttctttgtc aggccgtccg ggtgaatgct gccgtcaggg ctgttttttc
    28321 taatcccggc actgattaga taatcccccc tacttaccat gcggcactgt tcaactactg
    28381 caccaagtcg taaaccagcg acaggaaggc tgagtgacag ggggatagca atcatcattc
    28441 ctgtcttaat ttgcctgatg tggagacgat catcataaat atcactgaac cgcatattcg
    28501 ttatgtcttc gcgacgttgt cctgttacaa gggctaagtc catagctaat gggaaccatg
    28561 ccggaagttg atctgctgcc tccctgatgc agttgtatgt ctttagtttc agccgttctc
    28621 ttgtaactac tattttcggt gctcttgttg gcgttactgg attttgagat atacgtcctt
    28681 caacaatggc ttcgcggaac atatcagata acacagaacg cattgatcct gccatcgtgt
    28741 ttttccctcc ttcaatctac aaatcaagaa actcggcgat atggcgagtg gttatttctg
    28801 tcagtaaaac ccctcctaat ttttctttta ttgtctccag ttgatttacc cgagttttat
    28861 aagtatttct ggacgctttt ctccttataa gaatcgtttt gtaacgttca atccagcctg
    28921 ccatagtaaa tgagtcgacc cctttaagct tttcaattag ggcagcagga gagtagtttt
    28981 tgtatatata atgatttgct tcaattgcct gcgctactgc atctcttctt gaaattttac
    29041 ctagtgtaaa ttcttctttc gtcagagggt tgcgccagta atatgctttg tctctccttc
    29101 gatatgttaa gtttctaggc aaattgggat cgtatttttt ccgctgcatg ttttaacttc
    29161 tccattaacg gactgtctct cccttgtcgc ccattaggct gatggtgtgt tatatcggta
    29221 tcaaccttat ttgggttgat atagaaagcc tccggaacca ccctgtaact cctcccgtgt
    29281 agttcaggtg caggataaat gtttccattc cttgcccatc gtctcagcgt tgatattgat
    29341 ggtgggttat ccggatatct gagttttccc cacgttttga gtgtcacaag attcattgcc
    29401 atacctctca cgatatgacc gccagtaaat atacagaata ctggcgggtg tggttgattt
    29461 ttaata
//
LOCUS       DAJTUI010000044        29153 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTUI010000044 DAJTUI010000000
VERSION     DAJTUI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.044"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..206
                     /locus_tag="OO464_002110"
     CDS             <1..206
                     /locus_tag="OO464_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6227257.1"
                     /translation="HNPLNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALL
                     EGLKKQFDPNGIMNTGTIYPIEK"
     gene            228..1637
                     /locus_tag="OO464_002111"
     CDS             228..1637
                     /locus_tag="OO464_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6227258.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            1615..2394
                     /locus_tag="OO464_002112"
     CDS             1615..2394
                     /locus_tag="OO464_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6227259.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            2391..3251
                     /locus_tag="OO464_002113"
     CDS             2391..3251
                     /locus_tag="OO464_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6227260.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(3399..3974)
                     /locus_tag="OO464_002114"
                     /pseudo
     CDS             complement(3399..3974)
                     /locus_tag="OO464_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(3991..4251)
                     /locus_tag="OO464_002115"
     CDS             complement(3991..4251)
                     /locus_tag="OO464_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6227261.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4242..5513)
                     /locus_tag="OO464_002116"
     CDS             complement(4242..5513)
                     /locus_tag="OO464_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6227262.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(5591..5953)
                     /gene="queD"
                     /locus_tag="OO464_002117"
     CDS             complement(5591..5953)
                     /gene="queD"
                     /locus_tag="OO464_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6227263.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6272..8071
                     /gene="cysJ"
                     /locus_tag="OO464_002118"
     CDS             6272..8071
                     /gene="cysJ"
                     /locus_tag="OO464_002118"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6227264.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8071..9783
                     /gene="cysI"
                     /locus_tag="OO464_002119"
     CDS             8071..9783
                     /gene="cysI"
                     /locus_tag="OO464_002119"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6227265.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            9857..10591
                     /gene="cysH"
                     /locus_tag="OO464_002120"
     CDS             9857..10591
                     /gene="cysH"
                     /locus_tag="OO464_002120"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6227266.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            10855..11007
                     /locus_tag="OO464_002121"
     CDS             10855..11007
                     /locus_tag="OO464_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6227267.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11201..12292
                     /locus_tag="OO464_002122"
                     /pseudo
     CDS             11201..12292
                     /locus_tag="OO464_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(12438..13475)
                     /gene="iap"
                     /locus_tag="OO464_002123"
     CDS             complement(12438..13475)
                     /gene="iap"
                     /locus_tag="OO464_002123"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6227268.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            13727..14635
                     /gene="cysD"
                     /locus_tag="OO464_002124"
     CDS             13727..14635
                     /gene="cysD"
                     /locus_tag="OO464_002124"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6227269.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            14637..16064
                     /gene="cysN"
                     /locus_tag="OO464_002125"
     CDS             14637..16064
                     /gene="cysN"
                     /locus_tag="OO464_002125"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6227270.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16064..16669
                     /gene="cysC"
                     /locus_tag="OO464_002126"
     CDS             16064..16669
                     /gene="cysC"
                     /locus_tag="OO464_002126"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6227271.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            16719..17042
                     /locus_tag="OO464_002127"
     CDS             16719..17042
                     /locus_tag="OO464_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6227272.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17236..17547
                     /gene="ftsB"
                     /locus_tag="OO464_002128"
     CDS             17236..17547
                     /gene="ftsB"
                     /locus_tag="OO464_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6227273.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            17566..18276
                     /gene="ispD"
                     /locus_tag="OO464_002129"
     CDS             17566..18276
                     /gene="ispD"
                     /locus_tag="OO464_002129"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6227274.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18276..18755
                     /gene="ispF"
                     /locus_tag="OO464_002130"
     CDS             18276..18755
                     /gene="ispF"
                     /locus_tag="OO464_002130"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6227275.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            18752..19801
                     /gene="truD"
                     /locus_tag="OO464_002131"
     CDS             18752..19801
                     /gene="truD"
                     /locus_tag="OO464_002131"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6227276.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            19782..20543
                     /gene="surE"
                     /locus_tag="OO464_002132"
     CDS             19782..20543
                     /gene="surE"
                     /locus_tag="OO464_002132"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6227277.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            20537..21163
                     /gene="pcm"
                     /locus_tag="OO464_002133"
     CDS             20537..21163
                     /gene="pcm"
                     /locus_tag="OO464_002133"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6227278.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21303..22442
                     /gene="nlpD"
                     /locus_tag="OO464_002134"
     CDS             21303..22442
                     /gene="nlpD"
                     /locus_tag="OO464_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6227279.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            22505..23497
                     /gene="rpoS"
                     /locus_tag="OO464_002135"
     CDS             22505..23497
                     /gene="rpoS"
                     /locus_tag="OO464_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6227280.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(23617..24024)
                     /locus_tag="OO464_002136"
     CDS             complement(23617..24024)
                     /locus_tag="OO464_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR6227281.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24171..24764
                     /locus_tag="OO464_002137"
     CDS             24171..24764
                     /locus_tag="OO464_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR6227282.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            24764..25711
                     /locus_tag="OO464_002138"
                     /pseudo
     CDS             24764..25711
                     /locus_tag="OO464_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(25984..26547)
                     /locus_tag="OO464_002139"
     CDS             complement(25984..26547)
                     /locus_tag="OO464_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227283.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            26712..26963
                     /locus_tag="OO464_002140"
     CDS             26712..26963
                     /locus_tag="OO464_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6227284.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            26965..29061
                     /locus_tag="OO464_002141"
     CDS             26965..29061
                     /locus_tag="OO464_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6227285.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7120 a         7215 c         7904 g         6914 t
ORIGIN      
        1 accacaatcc gctcaacaaa atcatctgcg aagaaaccat tcgcctcggc ggttcgatgg
       61 tgcaccacca tggcattggt aaacatcgcg ttcactggag caagctggaa cacggcagcg
      121 cgtgggcgtt gctggaaggg ctgaaaaagc agttcgatcc taacggcatt atgaatacgg
      181 gtactatcta tccgattgaa aaataatgta tcaggcagcg ttccgcgatg acggggcgtt
      241 gccttttcgg cttctcaggc gagaagccgt tcttattacc ggacaatgaa ggggtaaaga
      301 tgaacacttc accggtgcga atggatgatt taccgcttaa ccgttttcac tgtcgaattg
      361 ctacgctcac tttcggcgca cacctgaccg acggttatgt tctaggcgtc attggttacg
      421 ccattattca acttacgccc gccatgcaac tgacgccgtt tatggcggga atgatcggcg
      481 gctcggcgct ccttggtttg tttcttggca gcctggttct tgggtggatc tccgaccata
      541 ttggtcggca aaaaatcttc accttcagct ttttgctgat tacgctcgct tcgttcttgc
      601 aattttttgc caccacgcca gagcatctta ttgggctgcg cattttgatc ggcattggtc
      661 tgggaggcga ttactcagta ggtcacacct tgctggctga attttccccg cgccgccatc
      721 gcggtatttt gctgggcgca ttcagcgtgg tgtggaccgt aggctatgtg ttggcaagta
      781 ttgccggaca tcactttatt tccgaaaacc cggaggcctg gcgctggttg ctggcatcgg
      841 cagctctgcc cgcgttgttg attacgttat tacgctgggg aacgccagaa tcgccacgtt
      901 ggctactgtg ccaggggcgt tttgcagaag ctcacgctat cgtgcatcgc tattttggtc
      961 cccatgtttt actgggcgat gaagtggtaa cggcgaccca taaacacatc aaaaccttgt
     1021 tctcttcgcg ttactggcgg cgcacggcgt ttaacagcgt cttctttgtc tgcctcgtaa
     1081 tcccatggtt tgtgatttat acctggctgc caactatcgc ccagactatt ggtctggaag
     1141 atgcgctgac tgccagcctg atgcttaatg cgttgttaat tgtgggcgcg ctgctgggat
     1201 tagttctgac gcacctgctg gcacatcgca aatttttgct gggaagtttt ttgctgctgg
     1261 cggcaacgct ggtagtaatg gcctgtttgc cttccggcag ttcattaacg ctgctgcttt
     1321 ttgttctctt cagcaccacc atttcggcag tcagtaatct ggtgggcatt ttgcctgcgg
     1381 aaagttttcc tactgacatt cgctcgctgg gagtcggttt tgccaccgcc atgagtcgac
     1441 ttggcgcggc ggtaagtact ggcctgctgc cgtgggtgct ggcgcagtgg ggaatgcaag
     1501 tcaccttatt gctcctagcg acagtgttgt tggttggttt tgttgtgacc tggctatggg
     1561 caccagaaac taaagccctc ccgctggtgg cggcgggaaa tgtaggaggt gcgaatgaac
     1621 attctgttag cgtttaaagc cgaaccggat gccggaatgc tggcggaaaa agagtggcag
     1681 gcggcggctc agggtaatag cggaccggat gtttcgctac tgcgaagttt actcggtgct
     1741 gatgaacagg ccgctgccgc gctgctgtta gcgcagagaa aaaacggtac gccgatgtct
     1801 ttaaccgcgt tgagcatggg ggatgaacgg gcgttgcact ggctgcgcta tctcatggct
     1861 cttgggtttg aggaagctgt attgctggag acggcggcag atctgcgctt tgcgccggaa
     1921 tttgtcgctc gccatattgc cgaatggcag cgtcagaatc cgctggatct gataattacc
     1981 ggcagccaaa gcagcgaagg gcagaatggg caaacgccat ttttgctggc ggaaatgctg
     2041 ggctggccct gcttcaccca ggtggagcgt ttcactctcg acgcgctgtt tatcaccctc
     2101 gaacagcgta ctgaacatgg gttgcgctgt tgccgggttc gcttgcctgc ggtgattgcc
     2161 gtgcgtcagt gcggtgaagt ggccttgcca gtaccgggaa tgcgccagcg aatggctgcg
     2221 gggaaagcag aaattatccg taaaactgtt gctgccgaaa tgccagcgat gcagtgtctg
     2281 caactggcaa gagcagagca acgacgcgga gcgacgctga ttgatgggca aacggtagca
     2341 gaaaaagctc aaaagctatg gcaggactat ttacgccaaa ggatgcagcc atgaatatcg
     2401 caattgtgac aataaatcag gagaatgccg caatagcgag ctggctggcg gcgcaggatt
     2461 tcagcggttg tacgctggcg cactggcaaa tagaacctca gcccgtagtg gcggaacagg
     2521 tgctggatgc gatggtggaa cagtggcagc gaacgcctgc ggacgttgtt ctctttccgc
     2581 cgggaacgtt tggcgatgaa ctgtcgaccc ggctggcctg gcgtttacat ggtgcaagca
     2641 tctgtcaggt gacttcgtta gatattccaa ccgtaagcat gcgcaaatcc cactggggaa
     2701 atgcgttaac ggcgacgctg caaacagaaa aacgcccgtt atgcctttcc ctggcgcgtc
     2761 aggcgggagc agcgaaaaac gccacgttgc cctctggaat gcagcaactg aacattgtcc
     2821 caggtgcgct tcccgactgg ctggttagca cagaggatct gaaaaatgtt agccgcgacc
     2881 cattagctga ggccagacgg gtgctggtgg tgggacaggg cggagaggca gataaccagg
     2941 aaatcgttat gctggcagaa aagctgggag ccgaagtggg ctacagtcgg gcgcgagtga
     3001 tgaacggtgg cgttgatgct gaaaaagtga ttggtatttc cggacatctg ctggcacctg
     3061 aggtgtgcat tgttgttggc gcttccggcg cagcagcgtt aatggcgggc gtacgcaata
     3121 gcaagtttat ggtggcgatt aatcatgacg ccagcgcagc ggtattttca caggcagata
     3181 ttggcgtggt tgatgactgg aaagtggtgc tggaggcact ggtgacaaac attcacgcag
     3241 aataccaata acgtgctgcc ggatgatcct gcctacggtt ctggactggg gacctgtagg
     3301 cctgataaga cgcgttaagc gtcgcatcag gcgctgaatg tcggatgtgg cgtgcggcca
     3361 acaaatcaaa acaaattcaa ttggttacga ttatcccgtc aaagcaattt tttcgctaac
     3421 gtccagactc cggtattcgt ggtggaaagc gccacgacac ccgcgttaat cgcattacgc
     3481 gcatcttctt catcgcatac cagcccgcca gcaatcagcg gttggcggat tttttccgtc
     3541 acccagccca gcactttggg catgcagcct ggcaggatct caatacaatc cggattcgat
     3601 tgcgcaactt acttatcaat gttgtgaaac gaaatcgaat caacaataaa caggtgatga
     3661 atacagaaaa aaacttccgc tcttgccgct tttagcatcg atgctttggt gctgataatt
     3721 ccatccgcct cggtcaccag tttcaaaaac tgaatcacca cttctttatt tgacgcgcct
     3781 tccagcaaat caacatgaat aaaagcatat ttcccggcgt tcttaatctt tttaacgata
     3841 ttactgatgg tgcagatatt gccgtacaac acggaaataa actggcattc agaatcgatg
     3901 gccagttgca ggctggcatt atctttaacg gcagcaatca ccggattctg gcggagcagg
     3961 tgtaagaggg gcataaatta tccttattat ttatccaaag cggtaggtga tgccgaatcc
     4021 ggaaggcgga tagcgccact gttgtagtgt ggattcgtcg cacagcaaac ggcaggtgcc
     4081 acactccagg cagccgcgat agtcaatacg taagtcacct tcctgtgtga gagaaaaaag
     4141 acctaccgga caggcgttaa tcaaccgttg taccatctga cgctcaacgg agtcagccgg
     4201 aacaatgtgc ggcgcatcag caacgcgcca gagattacgg gctacagaca tcgcagactc
     4261 ctgataacat cgcccgccag atgccacagg ccatgacgac gtaaatgatg ccagaataac
     4321 tggcgcagcg gtggaacagg tttatcaccc tgatcccata aatcgcggga aatatcctgc
     4381 attaacgcag gccacgcccg atacctgcca gggcgttgca aaagcgccgg aacatgctga
     4441 taacgttgta gaacatccca tagcaggctg cgctctacgt tgtgatgata aagcgcaaac
     4501 agattttgcg gctcgcggtg ctggcaggcg cttattagcg tttgtgccgc cgcctgtgcg
     4561 ccagtcagcg ccatatccat gccgcgcacg gaaattccgg tattgacgca actgcgcaac
     4621 gcatcgccca ccagcagcca gccgttaccg gcgtattgca ccggcatact gtgcaagcca
     4681 ccttctggca ccagatgcgc accatactcc agtgattccg tgtttttgat aagcgtgcgc
     4741 actgccggat gcgctttaaa gcgagtcagc agctcgcttg ccggaacacg actttgcgta
     4801 agggaagaga gcgggcaaac aatccctaac gagagcgttt gttgattagt ataaagaaat
     4861 gcgccgccgg gtaagtcatc acagattccg ccgctgaaca gcaacgctgc gccttcgtta
     4921 ttctccagat gaaaacgttc ttcaatagcg gatgtttcca gcgacagcac ttctttgatc
     4981 cccaacgcca tcgcttcgcc agcaggacga gtcactaacc cgtgacgttc agccaggacg
     5041 ctgttggcac cttctgccag caccacataa cgggcgcgga gaatatcgtc accacaaata
     5101 acgccacaga ctctgccgtt ttcttcatac agtgcatcca ccgtcgctcc ggggatgcat
     5161 tcgacacctt ctttttcggc ttcggcaacc agccacggat cgaaccgtgc acgtaataca
     5221 ttccaggatt caccgccggg ctgtaagctg gaaaacgtcg ttgcgccatc cggcgttaac
     5281 agggaaaggc tttcgtgagt gatgcgtcgt tcaagaggcg cggtcagatg aaattgcggg
     5341 aggagttccg cgagtgcatg ggtatataac cgcctgccgg aaagattttt gctgccgggg
     5401 atttcagcgc gttcgagtaa caaaacggat aaatccgctc gcgcgcagcg taacgcgcaa
     5461 gcggtgcctg caataccggc accaataata ataatgtcgc agtcgtcttc catgtcatgc
     5521 cccagatatc cagaacacaa caccctaaca tagcgttact aaagggaaat tgaccgccga
     5581 cacactctta tcattcgccg cgatagatac accctgcggt gcaggtttct tttaccatca
     5641 ccgcacttaa cagcggcaca acgggtttaa cctgatccca aatccatttt gctaaaactt
     5701 cgctggttgg attttccaga cctggaatat cattgagata atggtgatcg aggcgctcat
     5761 aagttggctt aaacgccgct ttcagttcag cgaaatcgat aatccagccc gtatgcggat
     5821 cgacttcccc ggtaatttcc agtcgcacca taaaggaatg cccgtgcagg cgaccacatt
     5881 tatgcccttc cgggacatgt ggtaagcggt gagcggcttc gaaggtgaaa tctttaaata
     5941 acgtggtgga catcataatt tctctacagg acgataaaac cgccgtagag taccggaaag
     6001 gctgtttttt cgcattatct aaaacgactt tgttgcgcaa aatcgctgat ttatcttaat
     6061 gattggctaa attcatttgt ttttcattag gttggttaat ctattttgtt gttaaagact
     6121 attgctaaaa caggttagtc gatttggtta ttagttatcg ctatcccgtc tttaatccac
     6181 accgttcacc ccgttaacct taccttctct tctgttttat gggcgctgac agggcgcaga
     6241 aacagctttg cttactggaa cataacgacg catgacgaca caggtcccac cttccgcgtt
     6301 gcttccgttg aacccggagc aactggcacg ccttcaggcg gccacgaccg atttaacgcc
     6361 tacccagctt gcctgggttt ctggctattt ctggggcgtg ctcaaccagc agcctgttgc
     6421 gcttgcagtg acgccagcgc ccgccgcaga aatgccgggt ataacgatta tctctgcttc
     6481 gcaaaccggc aatgcgcgcc gggttgctga agcattacgc gatgatttat tagcagcaaa
     6541 actgaacgtt aagctggtga acgcgggcga ctataaattc aaacaaatcg ccagcgaaaa
     6601 actgctcatc gtagtgacgt caacgcaagg ggaaggggaa ccgccggaag aagccgtcgc
     6661 gctgcataag ttcctgttct ccaaaaaagc gccaaagctg gaaaacaccg cgtttgccgt
     6721 gtttagcctc ggcgatagct cttatgaatt tttctgccag tccgggaaag attttgacag
     6781 caagctggcg gaactggggg gtgaacgcct gctcgaccgt gtcgattctg atgttgaata
     6841 ccaggctgct gccagcgagt ggcgcgcccg cgtggttgat gcgcttaaat cgcgtgcgcc
     6901 tgtcgcggca ccttcgcaat ccgtcgctac tggcgcggta aatgaaatcc acaccagccc
     6961 gtacagcaaa gacgcgccgc tggtggctag cctctctgtt aaccagaaaa ttactgggcg
     7021 taactctgaa aaagacgttc gccatatcga aattgactta ggcgactcgg gcctgcgtta
     7081 ccagccgggt gacgcgctgg gcgtctggta tcagaacgat ccggcactgg tgaaagaact
     7141 tgtcgaactg ctgtggctga caggcgatga acctgtcact gtcgagggca aaacgttgcc
     7201 tctgaacgaa gcgctacagt ggcacttcga actgaccgtc aacaccgcca acattgttga
     7261 gaattacgcc acgcttaccc gcagtgaaac actgctgccg ctggtgggcg ataaagcgaa
     7321 gttacagcat tacgccgcga cgacgctgat tgttgacatg gtgcgtttct ccccggcaca
     7381 gcttgatgcc gaagcgctaa ttaatctgct gcgcccgctg accccgcgtc tctattccat
     7441 tgcctcctcg caggcggaag tcgagaacga agtacacgtc accgttggtg tggtgcgtta
     7501 cgacgtggaa ggccgcgccc gtgccggtgg tgcctccagc ttcctcgctg accgcgtgga
     7561 agaagagggc gaagtccgcg tatttatcga acataacgat aacttccgcc tgcccgctaa
     7621 cctggaaacc ccggtgatta tgattggccc aggcaccggt attgcgccgt tccgcgcctt
     7681 tatgcagcaa cgcgccgctg acgaagcgcc aggtaaaaac tggctgttct ttggcaatcc
     7741 gcattttacg gaagacttcc tgtatcaggt ggagtggcag cgctacgtca aagagggtgt
     7801 gctgacgcgg atcgatctcg cctggtcgcg cgaccaaaaa gaaaaagttt acgtacaaga
     7861 taaactgcgc gaacagggcg cggagctgtg gcgctggatc aatgacggtg cccacattta
     7921 tgtctgcggc gacgctaatc gcatggcgaa agacgttgag caggcacttc tggaagtgat
     7981 tgccgaattt ggtggcatgg acaccgaagc ggcggatgaa tttttaagtg agctgcgcgt
     8041 agagcgccgt tatcagcgag atgtctacta atgagcgaaa aacatccagg gcctttagtg
     8101 gtcgaaggaa aactgacaga cgccgagcgc atgaagcttg aaagcaacta cctgcgcggc
     8161 accattgcgg aagatttaaa cgacggtctg accggcggct ttaagggcga taacttcctg
     8221 ctgatccgct tccacggcat gtatcagcag gatgaccgcg acatccgcgc cgaacgtgct
     8281 gaacagaagc tggagccgcg ccacgcgatg ctgttgcgct gccgtctgcc gggtggagtt
     8341 atcaccacca aacagtggca ggcgatcgat aaatttgccg gtgaaaacac catctatggc
     8401 agcattcgcc tgaccaaccg ccagacgttt cagttccacg gcattctgaa aaagaacgtc
     8461 aaaccggtgc accagatgct gcactcggtc ggtcttgatg cgctggcgac tgctaacgac
     8521 atgaaccgta acgtactctg cacctcgaac ccttacgagt cgcagctgca cgcagaagcg
     8581 tacgagtggg cgaagaagat ttctgaacat ctgctgccgc gtacccgcgc gtatgcggag
     8641 atctggctcg atcaggaaaa agtcgccacc accgatgaag aaccgatcct tggtcagact
     8701 tacctgccgc gtaagttcaa aaccacggta gtgatcccgc cgcagaacga tattgatctg
     8761 cacgccaacg acatgaactt cgtggcgatc gcggaaaacg gcaagctggt gggcttcaac
     8821 ctgctggtgg gcggtgggct ttctatcgaa cacggtaaca agaaaaccta cgcccgcacg
     8881 gcgagtgagt ttggctatct gccgctagag catacgctag cggtggcgga agccgtcgtg
     8941 acaactcagc gtgactgggg taaccgaacc gatcgtaaaa atgccaaaac caaatacaca
     9001 ctggagcgcg tgggggttga gacgtttaaa gcggaagtgg agcgtcgcgc ggggatcaaa
     9061 tttgaaccga tccgcccgta tgaatttaca ggacgcggcg atcgtattgg ctgggttaag
     9121 ggcattgatg ataactggca cctgacgctg tttatcgaaa atggtcgcat ccttgattat
     9181 ccggggcgtc cgctgaaaac tggcctgctg gagatcgcga agatccacaa aggtgatttc
     9241 cgcattacgg cgaaccagaa tctgatcatc gccggtgtgc cggaaagcga gaaagcgaag
     9301 atcgagaaaa ttgccaaaga gagcgggtta atgaatgccg tcacgccgca gcgtgaaaac
     9361 tcgatggcct gcgtgtcatt cccgacttgc ccgctggcga tggcggaagc ggagcgtttc
     9421 ctgccgtctt ttatcgacaa catcgataat ttaatggtga aacatggtgt cagcgatgag
     9481 catatcgtga tgcgtgtaac aggctgcccg aacggttgtg gtcgcgcgat gctggcggaa
     9541 gtgggcctgg tgggtaaagc accgggtcgc tacaacctgc atcttggcgg caaccgcatt
     9601 gggacacgta tcccacggat gtataaagaa aacatcaccg agccggaaat cctggcgtcg
     9661 cttgatgaac tgatagggcg ctgggcgaaa gagcgcgaag cgggtgaagg cttcggcgac
     9721 tttacggtgc gtgcgggcat cattcgcccg gtgctcgatc cggcgcgcga tttgtgggat
     9781 taaccatcag cccggtcttg taggcctgat aagacgcgtg agcgtcgcat caggcaaggc
     9841 aaacagtgag gaatctatgt ccaaactcga tctaaacgcc ctgaacgaac tgccgaaggt
     9901 agatcgcatt ctggcgctgg cagaaactaa cgccgaactg gaaaaactgg acgctgaagg
     9961 ccgcgttgcc tgggcgctgg ataatctgcc cggtgaatat gtactttctt ccagctttgg
    10021 cattcaggcg gcggtgagcc tgcatctggt gaatcagatt cacccggata ttccggtgat
    10081 cctcaccgac accggctatc tgttcccgga aacctaccgc tttattgacg agttaacgga
    10141 caaactcaag ctcaacctga aagtgtaccg cgctaccgaa agcgccgcct ggcaggaagc
    10201 acgctacggc aaactgtggg aacagggcgt tgaaggcatt gaaaagtaca atgacatcaa
    10261 caaagtcgaa ccgatgaacc gggcgctgaa agaactgaat gcgcaaacgt ggtttgctgg
    10321 cctgcggcgt gaacaatccg gcagccgtgc caatttaccg gtgctggcaa ttcagcgtgg
    10381 cgtatttaaa gtgctgccga ttatcgactg ggataaccga actatttatc agtacctgca
    10441 aaaacatggc ctgaaatatc acccattatg ggacgaagga tatttatcgg tgggcgatac
    10501 ccatacaacc cgtaaatggg aacccggcat ggcggaagaa gaaacgcgtt tctttggctt
    10561 aaaaagggaa tgtggattac acgaagggta aatattgccg gataaattaa acgcctctgt
    10621 cagaaatgat gggggcgttt ttattttgcg agcagcattc cggcatttaa aggtgaacat
    10681 cgcagggaaa attaacaggt atagaatggc gttgggtgct tgaggctgtc tggcgttaag
    10741 cattcgcgag gttccagatg gacaaaagcc ccaggcgata tttctatcaa cctgaggctc
    10801 gcgttcgaac gtagcaattc ggatgttagt ctcttctttg cagggaggca agacatgctg
    10861 acaaaatatg cccttgtggc aatcatcgta ctgtgttgta cagtactggg attcacgttg
    10921 atggtaggtg actcgttatg tgagttgagt atcagagaac gtggtatgga gtttaaggca
    10981 gttctcgctt acgaatcgaa gaagtagcca ccacgcgggg gtaaaacccc gcgtactgaa
    11041 ttgtgatggt ttggtctcct gcaccctttt tttaaattta tattttttat gtgaatatat
    11101 agtctctatt tattattatg aatttcaaat gaaaatcatt aatttattca taatgtaaat
    11161 gttttattgg ctgatatata tcatagatag tttttaaaat atgcgtaaat atcctttaag
    11221 tttactgaag gataaaaata ttgtgacttt ctttgatttc tggggaaaaa accgacgtgg
    11281 cgagaaagag ggtatcgacg actatcacct tctttgctgg catttgctgg atgtggccgc
    11341 aatgggctat ttaatggtta aaagaaattg cttcgggctg gctgattatt ttcgtcaatt
    11401 agggatttct gacaaggaac aggcagctcg atttttcgcc tggttgctgt gctggcacga
    11461 tattggaaaa tttgcccgct cttttcaaca actttacctg ccccctgaat tcaagattca
    11521 ggaaggtgcc agaaaaaatt acgaaaaaat ctctcattca acgctgggct actggttgtg
    11581 gaatcattat ttaagtgaat gtcaggagtt acttccttca tcttcgctct ctccgcgcaa
    11641 acttaggcga gtgatagaaa tgtggatgcc cgtgacgaca gggcatcatg gtcggccccc
    11701 tgatcgtatg gatgaattgg ataattttct gcctgaagac aaagctgcag cacgagattt
    11761 tctccttgaa atcaagccac tgtttccgcg aatagagatc cccacattct gggatgatga
    11821 cgagggcgtt gaacttataa aacaactttc ctggtatatc tctgcaaccg tcgtacttgc
    11881 agactggaca gggtcgtcaa cgcgattttt tccacgcgtc gcacaggcaa tggatattga
    11941 agattactgg caaaaggctt tagttcaggc tcaaaacgca ttaaccgtct ttccgccaca
    12001 agcagaaacc gcgcctttca ccggaattaa tacgctgttt ccttttattg agaatacgac
    12061 accgttacag caaaaggtgc tggatctgga tatcaatcag ccaggaccac agttgtttat
    12121 tctggaagat gtgaccggcg caggtaaaac agaagcggcg cttatcctgg cgcacaggtt
    12181 gatggctgcg ggtaacgctc aggggctgtt ttttggcctg ccaacaatgg caacggccaa
    12241 tgccatgtac gatcgactgg tcaaaacctg gcttgctttc tattcgccag aggtgttccc
    12301 cgctccagcg gagataaacc ggcgcaatac ggtgcggttt tcccggaagt aaaccggacg
    12361 cactggatgc gatgatgggt atcacttaga attccccgcc cctgcggtag aactcccagc
    12421 tcccattttc aaacccatca agacgccttc gccagctcct tcaccaacgg tagcattatc
    12481 cgcataacgt cacggcagcg acgttctatt cttccaggaa gtgccttatc aatatgttgt
    12541 tgattatcca gtcttacgtc atgccagcta tttcccgccg ggaaggcagg tgtttttgcg
    12601 cgttgctgat aaccatcctt atttccaaga ttccagttag tcgcttccac cgaaagtaca
    12661 gcaatgcccg ctttgtcgaa tatttctgcg tcattacaac ccccagtgcc tttcggataa
    12721 tttttattca aacccggatt ggtcgttgcg gctattccgt gactgcgtgc aattgccagc
    12781 gccctgtcgc gcgttaattt ccttactgct tcaggggttt ttacaccgct gttgaaatac
    12841 aatttatcgc caacaattaa gttatcgaga ttaatcacca gcagcgtatt tttcttttcg
    12901 gtgtcactca tccgcttgag taaattctca gcgcctaatt ttccttcctc ttcgccgctg
    12961 gtcgccacaa atcgaatacc atactcggta ggcgtatttt tcaggcgttc tgccagttcc
    13021 agcatgacac ctaaacctgc ggcgttatca tctattcctt gtaacgtcag tccgccgaga
    13081 ttggcatctg cgtcagcatc gctcagcggg gcgtaagtat ccagatgcgc cataatgatg
    13141 atctgctgcg gcgctttgcc ttcatgagcg gcaatcaccg tacttcctgt cacgttatgc
    13201 cagttcttgc gattatcgcg ggcggtataa atataccgac tattaaatgt ccgaatatca
    13261 ctgcgataac ccatttgctg aaactgttgg cgaatataat cggcagataa catttctgct
    13321 ggagttccgg tcatgcgtcc cggaaagaaa gtagcaatat gtcgtgcctg ggtattagca
    13381 aaatcgccag gtttaggtga cgaggcgtgt acggggagaa taaagcatac gccgagcgcc
    13441 agggcagcgg tacggtggcg caatgcggaa aacatagtga gtccttaaat accatgcaaa
    13501 tttttttacc gccatagtat gaaactgccg ctgcgctaaa acaatttcaa atcttcctaa
    13561 acgcccgaaa tccggtgcct taagcacttt ttgatattag ctttgccaaa tcgttattcc
    13621 gttaaggaac tactcattct aattggtaat ttcattcgtt ctcttacgct ccctatagtc
    13681 gaaacatctg atggcaagaa aatagcggta ttgcaaagga acggttatgg ctcaaaaacg
    13741 acttactcac ctgcggcaac tggaggcgga aagcatccac attattcgcg aggtggcggc
    13801 agaattctca aatccggtga tgctctactc tatcggtaaa gattccagcg tcatgctgca
    13861 tctggcgcgc aaggcgtttt atccaggtac gctgcctttc ccgttgctgc atgtcgatac
    13921 cggctggaaa ttccgcgaga tgtatgagtt ccgcgatcgt acggcgaaag cctacggctg
    13981 cgagctgctg gtgcataaaa acccggaagg cgtggcgatg gggattaatc cattcgtgca
    14041 cggcagcgcg aaacataccg atattatgaa aactgaaggc ctgaaacagg cgctgaacaa
    14101 atacggtttt gatgccgcct tcggtggtgc gcgccgtgac gaagagaaat cccgcgctaa
    14161 agagcgaatc tactctttcc gtgaccgctt ccatcgctgg gatccgaaaa atcagcgccc
    14221 ggagctgtgg cacaactaca acgggcaaat taacaaaggc gaaagcatcc gcgtcttccc
    14281 gctctctaac tggaccgagc aggatatctg gcaatacatc tggctggaaa atatcgacat
    14341 tgtgccgctg tatctcgctg cggaacgtcc ggttctggaa cgcgacggta tgttgatgat
    14401 gattgatgac aaccgtatcg acctgcaacc gggcgaagtg atcaaaaaac ggatggtgcg
    14461 tttccgtacg ctgggctgct ggccgctgac cggtgcggtg gagtcaaatg cacaaacact
    14521 gccggaaatc atcgaagaga tgctggtttc caccaccagt gaacgtcagg gtcgcgtgat
    14581 tgaccgcgac caggcgggat ccatggagct gaaaaaacgt caggggtatt tttaagatga
    14641 acaccgtact tgcacaacaa atcgccaatg aaggcggcgt cgaagcctgg atgattgcgc
    14701 aacaacataa aagcctgctg cgttttctga cctgtggtag cgtcgatgac ggcaaaagta
    14761 ccctgattgg tcgtctgctg cacgataccc gccaaatcta cgaagatcag ctctcatcgc
    14821 tgcataacga cagtaagcgt cacggaaccc agggcgaaaa gctggatctg gctctgctgg
    14881 tggacggcct gcaagctgag cgcgaacagg gcatcactat tgatgtggcc taccgctatt
    14941 tctctaccga gaagcgtaaa tttattatcg ccgacacccc agggcacgag cagtacactc
    15001 gcaatatggc gactggcgca tcgacatgtg aactggcgat cttactgatt gatgcccgta
    15061 aaggcgtgct cgatcaaacc cgtcgtcaca gttttatctc cacactgttg gggatcaaac
    15121 atctggtcgt ggcgatcaac aaaatggatc tggtggatta cagtgaagag acgttcaccc
    15181 gtattcgtga agattatctg acctttgccg ggcagctgcc gggtaatctg gatatccgct
    15241 ttgtgccgct ctccgcactg gaaggtgaca acgtggcttc gcaaagtgaa agtatgccgt
    15301 ggtacagcgg tctgacactg ctcgaagtgc tggaaaccgt ggagatccag cgagtggtgg
    15361 atgcccagcc aatgcgcttc ccggtgcagt acgttaaccg cccaaatctc gattttcgtg
    15421 gctacgccgg aatgctggca tccggtcgcg tggaagtcgg gcaacgtgta aaagtgctgc
    15481 cctctggtgt ggaatcaaac gtcgcgcgga tcgtgacttt tgatggtgat cgcgaagaag
    15541 tctttgccgg agaagcgatc accctggtgc tgacggatga gatcgatatc agccgtggtg
    15601 atctgctgct ggcggcagac gaagcgttac cggcggtgca gagtgcgtcg gtggatgtgg
    15661 tatggatggc ggaacagccg ctttccccgg gccagagtta tgacatcaaa attgccggta
    15721 agaagacgcg tgctcgtgtt gatggcattc gttatcaggt tgatattaat aaccttaccc
    15781 aacgcgaagt tgaaaacctg ccgctgaacg gcatcggcct ggtggatctc acttttgacg
    15841 agccactggt gttagatcgc tatcagcaaa atccggttac cggtgggctg atttttatcg
    15901 atcgcctgag caatgtgacc gtgggtgccg gtatggtgca cgagccagtt agccaggcaa
    15961 cagctgcgtc atctgaattc agtgcattcg aactggaatt gaatgccctg gttcgtcgcc
    16021 actttccgca ctggggcgcg cgcgatttgc tgggggataa ataatggcgc tgcatgacga
    16081 aaacgtcgtc tggcatagtc atccggtcac tgtgcaacaa cgcgaactac accacggtca
    16141 tcgtggtgta gtgctgtggt ttaccggcct ctccgggtcc ggtaaatcaa cggtcgccgg
    16201 ggcgctggag gaggcgttac ataaactcag cgtcagtacg tatctgctgg atggcgacaa
    16261 tgttcgccac ggattatgca gcgatctcgg ttttagcgat gccgatcgta aagagaatat
    16321 ccgtcgcgtc ggtgaagtgg cgaatttgat ggttgaagcc ggactggtgg tgctgaccgc
    16381 atttatctcg ccacaccgcg ccgaacgcca gatggttcgc gaacgcgtag gagaagggcg
    16441 ctttattgaa gtgtttgtcg atacgccgct ggcgatttgc gaagcccgcg atccaaaagg
    16501 cttatataag aaagcgcgtg ccggtgaact gcgcaacttt acgggaatag attccgttta
    16561 cgaagcgcct gaatcggcag aaattcatct caatggtgaa caattagtaa caaatttggt
    16621 acagcaatta ttagatctgt tgagacagaa cgatattatc agatcctgag acaccaccgg
    16681 gtttccatgc ccggttcgtc aaagtaacag gatcaaatat gcgtaatagc cataacatta
    16741 cactaacaaa taacgacagc cttactgagg atgaagaaac tacatggtca ctgcctggtg
    16801 ccgtggtcgg ttttatctcc tggttatttg cgctggcgat gccgatgttg atttatggct
    16861 ctaacacgct gttcttcttt ctctacacct ggcctttctt tctggcactg atgcctgtcg
    16921 cggtagtggt ggggattgcg ctgcattcat tgatggacgg aaagctacgc tacagtattg
    16981 ttttcaccct ggtgacagta ggcattatgt ttggcgcact gtttatgtgg ctactgggct
    17041 aatttgtact ttcttgtctc ctctgttcat aattcaaacc gtaactaata atgagattat
    17101 gttctgcacg ccctgggtat acgtaacaat gggcaaatgt ggtacatttg cccgcgttgt
    17161 cgcggtatcc ccaacagagg atgtagagtc gtcttcggat gcatgggatg atgatgcagt
    17221 ttttcagggg gcaggatggg taaactaacg ctgctgttgc tggctattct ggtctggcta
    17281 cagtattcgc tgtggttcgg taagaacggt atacatgact atacccgcgt caatgatgat
    17341 gtggcggcac tgcaagctac aaacgcgaaa cttaaagcgc gaaacgatca actttttgcc
    17401 gaaattgacg atctcaatgg cggccaggag gcgctcgaag agcgtgcgcg taatgaactc
    17461 agcatgacca ggccgggcga aactttttat cgtctggtgc ctgacgcgtc gaagcgcgca
    17521 cagtctgcgg ggcaaaacaa tcgataaatc agcccgggaa ttaacatggc aaccactcat
    17581 ttggatgttt gcgccgtggt tccggcggcc ggatttggcc gtcgaatgca aacggaatgt
    17641 cctaagcaat atctctcaat cggtaatcaa accattcttg aacactcggt gcatgcgctg
    17701 ctggcgcatc cccgggtgac acgtgtcgtc attgccataa gtcctggcga tagccgtttt
    17761 gcacaacttc ctctggcgaa tcatccgcaa atcaccgttg tagatggcgg tgatgagcgt
    17821 gccgattccg tgctggcagg tctgaaagcc gctggcgatg tgcagtgggt attggtgcat
    17881 gacgccgctc gtccttgttt gcatcaggat gaccttgcgc gattgttggc gttgagcgaa
    17941 accagccgca cgggagggat cctcgccgca ccagtgcgcg atactatgaa acgtgccgaa
    18001 ccgggcaaaa atgccattgc tcataccgtt gatcgcaacg gcttatggca cgcgctgacg
    18061 ccgcaatttt tccctcgtga gctgttacat gactgtctga cgcgcgctct aaatgaaggc
    18121 gctaccatta ccgacgaagc ctcggcgctg gaatattgcg gatttcatcc gcagttggtc
    18181 gaaggccgcg cggataacat taaagtcacg cgcccggaag atttggcact ggccgagttt
    18241 tacctcaccc gaaccatcca tcaggagaat acataatgcg aattggacac ggttttgacg
    18301 tacacgcctt tggcggtgaa ggcccaatta tcattggtgg cgtacgcatt ccttacgaaa
    18361 aaggattgct ggcgcattct gatggcgacg tggcgcttca tgcgttgacc gatgcattgc
    18421 ttggcgcggc ggcgctgggg gatatcggca aactgttccc ggataccgat ccggcattta
    18481 aaggtgccga cagccgcgag ctgctacgcg aagcctggcg tcgtattcag gcgaagggtt
    18541 atacccttgg caacgtcgat gtcactatca tcgctcaggc accgaagatg ttgccgcaca
    18601 ttccacaaat gcgcgtgttt attgccgaag atctcggctg ccatatggat gatgttaacg
    18661 tgaaagccac tactacggaa aaacttggat ttaccggacg tggggaaggg attgcctgtg
    18721 aagcggtggc gctactcatt aaggcaacaa aatgattgag tttgataatc tcacttacct
    18781 ccacggtaaa ccgcaaggca ccgggctgct gaaagccaat ccggaagact ttgtggtggt
    18841 ggaagatttg ggctttgagc ctgatggtga aggtgagcat attctggtta gaatcctcaa
    18901 aaacggctgc aatacccgtt ttgtggcgga tgcactggcg aaattcctga aaattcattc
    18961 ccgtgaagtc agcttcgctg ggcaaaaaga caaacatgct gtaacggaac agtggttatg
    19021 tgctcgcgtg ccgggcaagg aaatgcccga tctgagcgcc tttcaactgg aaggctgcca
    19081 ggtgctggag tatgcgcggc acaagcgcaa gctgcgttta ggggcgctga aaggtaacgc
    19141 ctttaccctg gttctgcgcg aagtgagcaa tcgcgatgac gttgaacaac gtctgatcga
    19201 tatttgcgta aaaggtgtac cgaactactt cggtgcccaa cgttttggga ttggcggtag
    19261 caacttgcag ggcgcgctgc gctgggcgca aaccaatact ccggtgcgcg atcgcaataa
    19321 acggagtttt tggttgtcgg cagcccgcag tgcgttgttt aatcagattg ttgctgagcg
    19381 cctcaaaaaa gcagacgtta atcaagttgt tgacggcgat gcgctacaat tagccggacg
    19441 tggtagctgg tttgtagcaa ccaccgaaga actggcggaa ttacagcgtc gcgtcaacga
    19501 taaagagctg atgataaccg ccgcattgcc gggcagtggc gaatggggaa ctcagcgtga
    19561 agcgctggca ttcgaacaag cagctgtcgc cgcagaaact gaattacaag ctttactggt
    19621 gcgcgaaaaa gttgaagccg cgcgcagagc gatgctgctg tatccgcaac aattaagctg
    19681 gaactggtgg gatgacgtca ccgtagagat ccgtttctgg cttccggcgg gtagttttgc
    19741 aaccagcgtt gtcagggaac ttatcaacac aacaggtgat tatgcgcata ttgctgagta
    19801 attatgacgg ggtacatgca cccggtatac aaacgctggc gaaagccttg cgtgagtttg
    19861 ctgacgttca ggtggtcgcc cccgatcgta accgcagcgg cgcttcaaat tctctgacac
    19921 tggaatcctc cctgcgcacg tttacctttg aaaatggtga tattgctgtg caaatgggaa
    19981 ccccgaccga ttgtgtctat cttggcgtga atgctctgat gcgtccgcgc ccggacattg
    20041 ttgtgtccgg aattaacgcc gggccgaatc tgggggatga tgttatttat tccggtacgg
    20101 tagccgccgc gatggaaggc cgtcatttag gttttccggc gctggccgtc tcgcttgacg
    20161 ggcataaaca ttacgacact gccgcggcgg taacctgttc aattttgcgc gcactgtgta
    20221 aagagccgct gcgcaccgga cgtattctta atattaacgt tcaggattta cccttggatc
    20281 aaatcaaagg tattcgcgtg acgcgctgcg gtacacgaca tccggcagat caggtgatcc
    20341 cgcagcaaga tccgcgcggc aatacgctgt actggattgg cccgccgggc ggtaaatgtg
    20401 atgccggtcc ggggaccgat tttgctgcgg tagatgaggg ctatgtctcc atcacgccgc
    20461 tgcatgtgga tttaactgcg catagcgcgc aagatgtggt ttcagactgg ttaaacagcg
    20521 tgggagttgg cacgcaatgg taagcagacg cgtacaagca cttctggatc aattacgtgc
    20581 gcaaggtatt caggatgagc aggtgctgaa tgcacttgcc gccgtgccgc gtgaaaaatt
    20641 cgttgatgaa gcgtttgaac aaaaagcctg ggacaatatc gccttgccga taggtcaggg
    20701 gcagacaatt tcgcagccgt atatggtggc gcgaatgacc gaattactcg agctgacgcc
    20761 gcagtcgcgg gtgctggaaa ttggcactgg ttcgggatat caaacggcaa tcctggcgca
    20821 tcttgtccag catgtttgct cggttgaacg gattaaaggc ttgcagtggc aggcacgtcg
    20881 ccgcctgaaa aatcttgatt tacataatgt ttcaacccgt catggtgatg gatggcaagg
    20941 ttggcaggca cgtgcgccgt ttgacgctat cattgttacg gcggcaccgc cggaaattcc
    21001 aactgcgcta atgacgcagc tggacgaagg cgggattctc gtcttacccg taggggagga
    21061 gcaccagtat ttgaaacggg tgcgtcgtcg gggaggcgaa tttattatcg ataccgtgga
    21121 ggccgtgcgc tttgtccctt tagtgaaggg tgagctggct taaaacgtga ggaaatacct
    21181 ggatttttcc tggttatttt gccgtaggtc agcgtatcgt gaacatcttt tccagtgttc
    21241 agtagggtgc cttgcacggt aattatgtca ctggttatta accaattttt cctgggggat
    21301 aaatgagcgc gggaagccca aaattcaccg ttcgccgcat tgcggctttg tcactggttt
    21361 cgctatggct ggcaggctgt tctgacactt caaatccacc ggcaccggtc agctccgtta
    21421 atggcaatgc gcctgcaaat actaattctg gtatgttgat tacgccgccg ccgaaaatgg
    21481 ggacgacgtc tacagcgcag caaccgcaaa ttcagccggt acagcagcca caaattcagg
    21541 ctactcaaca accgcaaatc cagccagtgc agccagtagc tcagcagccg gtacagatgg
    21601 aaaacggacg catcgtctat aaccgtcagt atgggaacat tccgaaaggc agttatagcg
    21661 gcagtaccta taccgtgaaa aaaggcgaca cacttttcta tatcgcctgg attactggca
    21721 acgatttccg tgaccttgct cagcgcaaca atattcaggc accatacgcg ctgaacgttg
    21781 gtcagacctt gcaggtgggt aatgcttccg gtacgccaat cactggcgga aatgccatta
    21841 cccaggccga cgcagcagag caaggagttg tgatcaagcc tgcacaaaat tccaccgttg
    21901 ctgttgcgtc gcaaccgaca attacgtatt ctgagtcttc gggtgaacag agtgctaaca
    21961 aaatgttgcc gaacaacaag ccaactgcga ccacggtcac agcgcctgta acggtaccaa
    22021 cagcaagcac aaccgagccg actgtcagca gtacatcaac cagtacgcct atctccacct
    22081 ggcgctggcc gactgagggc aaagtgatcg aaacctttgg cgcttctgag gggggcaaca
    22141 aggggattga tatcgcaggc agcaaaggac aggcaattat cgcgaccgca gatggccgcg
    22201 ttgtttatgc tggtaacgcg ctgcgcggct acggtaatct gattatcatc aaacataatg
    22261 atgattacct gagtgcctac gcccataacg acacaatgct ggtccgggaa caacaagaag
    22321 tgaaggcggg gcaaaaaata gcaaccatgg gtagcaccgg aaccagttca acacgcttgc
    22381 attttgaaat tcgttacaag gggaaatccg taaacccgct gcgttatttg ccgcagcgat
    22441 aaatcggcgg aaccaggctt ttgcttgaat gttccgtcaa gggatcacgg gtaggagcca
    22501 ccttatgagt cagaatacgc tgaaagttca tgatttaaat gaagatgcgg aatttgatga
    22561 gaacggagtt gaggtttttg acgaaaaggc cttagtagaa gaggaaccca gtgataacga
    22621 tttggccgaa gaggaactgt tatcgcaggg agccacacag cgtgtgttgg acgcgactca
    22681 gctttacctt ggtgagattg gttattcacc actgttaacg gccgaagaag aagtttattt
    22741 tgcgcgtcgc gcactgcgtg gagatgtcgc ctctcgccgc cggatgatcg agagtaactt
    22801 gcgtctggtg gtaaaaattg cccgccgtta tggcaatcgt ggtctggcgt tgctggacct
    22861 tatcgaagag ggcaacttgg ggctgatccg cgcggtagag aagtttgacc cggaacgtgg
    22921 tttccgcttc tcaacatacg caacctggtg gattcgccag acgattgaac gggcgattat
    22981 gaaccaaacc cgtactattc gtttgccgat tcacatcgta aaggagctga acgtttacct
    23041 gcgaaccgca cgtgagttgt cccataagct ggaccatgaa ccaagtgcgg aagagatcgc
    23101 agagcaactg gataagccag ttgatgacgt cagccgtatg cttcgtctta acgagcgcat
    23161 tacctcggta gacaccccgc tgggtggtga ttccgaaaaa gcgttgctgg acatcctggc
    23221 cgatgaaaaa gagaacggtc cggaagatac cacgcaagat gacgatatga agcagagcat
    23281 cgtcaaatgg ctgttcgagc tgaacgccaa acagcgtgaa gtactggcac gtcgattcgg
    23341 tttgctgggg tatgaagcgg caacactgga agatgtaggt cgtgaaattg gcctcacccg
    23401 tgaacgtgtt cgccagattc aggttgaagg cctgcgccgt ttgcgcgaaa tcctgcaaac
    23461 gcaggggctg aatatcgaag cgctgttccg caagtaagta agcatctgtc agaaaggcca
    23521 gtctcaagcg aggctggcct ttttcttttg tttgggaagc ttgtgttttt gtcgcctgga
    23581 taagacacgt cagcgtccta cccggcaatc agcattctat gtgctcatca tcttgcgcac
    23641 caactgcata aacagctcct gctcttctcc actcaaacgc cccaaaaatt cctcatccac
    23701 gctgtcacca ataggtatcg ccgcagcaag tagcttttct ccttcggcag tcagccagac
    23761 aaagcgtcgc cgcttgtcag cgacgtcatg ttcacgtctg actaagccac gattttccat
    23821 tcttgccaac atttctgcta gcgttgcttt ggtgctgact gctgcttcta tcagtgccac
    23881 ctgttcaata ccaggtttat cagcaatggc gcgcatcacc gcgtactgtg gtttggtcag
    23941 gtcaggtaac tcgtgctgcc agcgagccgt gtgctgctga aaaagctgtc gtaactgatg
    24001 gaacgcttta tttcgtaacg ccatgtaaac tccgggctaa ttctctgtcg ctatcatagc
    24061 ggttttttag tgacgaagaa gatgttgtta cttttcaatc cttgccgctg tggaaatgaa
    24121 ggagtatgtt aataataacg ttcgtatacg aataattaag agaataagca atgaaactga
    24181 tcgtcgggat gacaggggct accggtgcgc ctcttggtgt ggcattactg caagcgctgc
    24241 gtgagatgcc gaatgtcgag actcatctgg tgatgtcgaa gtgggcgaaa accaccattg
    24301 aactggaaac gccttacagc gctcgcgatg ttgctgccct cgcagacttc agccataacc
    24361 cggcggatca ggcggcgatc atctcatccg gttcttttcg taccgacggc atgatcgtta
    24421 ttccgtgcag tatgaaaacg ctcgccggta tccgcgctgg ttacgccgat ggcctggtag
    24481 ggcgcgcggc ggacgtcgtg ctcaaagaag gccgcaaact ggtgctggtg ccgcgtgaaa
    24541 tgccgcttag caccatccat ctcgaaaata tgctcgcact ttcacgcatg ggcgtggcga
    24601 tggtgccgcc gatgcctgcc ttttataacc atcccgaaac ggtagatgac attgtccacc
    24661 atgtggtagc ccgcgtgctg gatcaatttg gcctcgaaca tccccacgcc aggcgctggc
    24721 aaggattgcc gcaggcccgg aatttttctc aggagaatga ataatggcat ttgatgattt
    24781 acgcagcttt ttacaggcgc ttgatgacca cggccagtta ctgaaaatca gcgaagaagt
    24841 gaacgctgag ccggatctgg cagcagcagc taacgccacc gggcgtatcg gcgacggcgc
    24901 gcccgcgctg tggtttgata atattcgcgg ctttaccgat gcccgcgtgg cgatgaatac
    24961 catcggttcc tggcagaacc acgcgatttc ccttggcctg ccaccaaaca ccccggtcaa
    25021 aaagcagatt gatgagttta tccgccgctg ggataacttc ccgattgccc cggagcgccg
    25081 cgccaatcca gcctgggcgc agaacaccgt cgatggcgaa gagatcaacc tgttcgatat
    25141 tctgccgctg tttcgcctga atgatggcga tggcggtttc tatctcgaca aagcgtgcgt
    25201 ggtttcccgc gatccgctcg acccggataa cttcggcaag cagaacgtcg gcatctaccg
    25261 catggaagtg aagggcaagc gtaagctcgg cctgcaaccg gtgccgatgc acgatatcgc
    25321 cctgcatctg cataaagcag aagagcgcgg tgaagatctg ccgattgcga tcacgctcgg
    25381 taacgatccg atcatcacgc tgatgggggc cacgccgctg aaatacgatc agtctgagta
    25441 tgaaatggca ggcgcgctgc gtgaaagccc gtacccgatc gccaccgctc cgctgaccgg
    25501 ttttgatgtg ccgtggggtt cagaagtgat cctcgaaggg gttatcgaaa gccgtaaacg
    25561 cgaaatcgaa gggccgttcg gtgagtttac cgggcactac tccggtgggc gcaacatgac
    25621 cgtggtgcgc atcgataaag tctcttatcg cagcaaaccg attttcgaat cactctatct
    25681 cggtatgccg tggaccgaaa tcgactacct atgactaggt aatttattca agataaccgg
    25741 gaagagatta aaaaagacgg gaatagattg gaatcaacaa gttaggaact caaggttttc
    25801 aagataaagc gagtccaaaa atgttaaacg gttcgatatc aataaaacat tcaagataac
    25861 tcacaggttc ttacaatagc ctttaaagac cgttcaaaaa cgattcaggt gtttattata
    25921 gcgctgatgg gtgcaagcgc caaaaccaat gtaattttag cacagctagg cacttgcact
    25981 gggttatgcg ctggctgctt tctgaggtag atttaagtga tcgcccttag cttcggacaa
    26041 cgcgtaaatc tctctaacct tttcgtctgg caaattacca aggcttaacg ctatggctct
    26101 acgaactcca actagagcgt gcaactcttg gatgtgctca tccagaagaa tgagtaattg
    26161 ctccgctcct tttctcccaa gaacatcagc tacttgttgt ttttcttgtg gtttaagaaa
    26221 tgacaagaac attagaattt gagcatcaac attatccgca ttctcttttt tgggttcttt
    26281 ggaatctttt agttcaacat caccctctcc cgtcatgagc cattccaagg taacattttc
    26341 gctttctgct atctttttta actgatacgg gcgtggtatc ggtgcgtaat cccgcctgtt
    26401 gtaataattt tttaaagtgc cttcattgat cccccatgcc cgagcagccg catttcttga
    26461 agggtatcgt tctactaacc taaagattct ttcgataatc ggttcttttc catcggcagg
    26521 aaaagaacct tggggtgctt ttctcattct tggttccttt ctatctaatt cattgataaa
    26581 taataaaatt aaaaaataaa ccataaaaac accaaagaac cctttgcagg gaatctttgg
    26641 tatcttaatc tttatccata cggatatccg accggatatc cgcgcgggta attttgtagg
    26701 ttaaaggaaa gatgagacaa agagaagata gtgaccgcga ttggccttcg ctgaagattc
    26761 agctggcgct caaagataac gggtggtctt ccttccggga gctttcactc catgctggtt
    26821 atgctcagga ttcactgaaa gcggtagttc gcacaccaaa caaaaaatac gaacgcgtaa
    26881 tttctgacgc tataggtgta ccgccagaaa tcatctggcc cagccggtat cgtaacgatg
    26941 gatttatcag gaaggcgtcg tgatatgcag tcgtacgtta ccgtaaatga cctgctcggc
    27001 gtaccaggaa tgccagccac cacaaaaggc atccgacagg cgttacagcg tttttctcgt
    27061 gatcttggtg acgttagtcg ccgtcgggaa ggcacaaaag ccattgagta ccacattgat
    27121 tgtctgcctg aaataacacg aaaggcactg cgtgaacgct atgtggaaca gctggtggct
    27181 acagagaaca atgtttctga agtgaaagct gttaccagaa aaacacgcaa tcctgacgct
    27241 gtccaggcaa tcgaagcata tcgcggttct ccacaactga tggaagaacg cctgaatgcg
    27301 ctgaccgaaa accagcgctg ggtatctgaa gcaagagctg cgctggtggt ggaagtgctg
    27361 aagctggaaa gcgccggtaa ccccgggcga ctgaaagcca ttaactttct tgttgaaaaa
    27421 gcccgtaaag gtgagctgcc ggagcgcctg caacaggccg cagttaacgc caatgcaaaa
    27481 cgtggcgcta atcgtactat cagccgcgat ccactttacc agtgggttct gaaatacaac
    27541 cagtcgcaga acgccgccga acggctgctg ttactggcac cgggaaaacg cgacgaaata
    27601 aaaccggaag aaatcagctg gctgcctgaa tttctggcgc agtatcgcca ggtgaacggc
    27661 agacccatgt cagaagccta cgaggatttt gtcgcagaat ggcagcgtcg ccatgcggac
    27721 gaaccgtaca tgctggaggt gatgccttcc tatgatgttg tccggtatgc catgaaaaaa
    27781 ctgccggaag tggtgaaaca aaaagggcgc gttaccggca gtgaataccg tcagcttgaa
    27841 ggattcaccc gccgcgactg gacagctatg ccggtgaatt atgtctggat tggtgacggt
    27901 cacggtatga agctgaaatg tgcgcatccg atccacgggc gtccattctc accggaagtc
    27961 acgtttgtga tcgatggtgg aacacggttt gttgttggct ggagtcttga ccttgccgaa
    28021 aacgtgttcg ccgttgccgg tgcgatacag cacggcattc gcaaccacgg aaaaccgttc
    28081 ctgtattact cggataacgg ttccggtgaa acggctgaca tgctggacaa agaggtcgtg
    28141 ggtattctgc cacgcctggg gattaaacac cctaccggta ttgccggtaa tccacaggga
    28201 cgcggcatta ttgaacgcct gaaccggaca ctgccgatgc gtatagccag gaggtatcgc
    28261 acctattttg gcaagggtgc tgaccgggaa tcgttgcgtg tacttaaccg tgatctgcgt
    28321 tcagcgttta acgccctgca acaggacaag cctctgaacg accgccagaa agcggcaatg
    28381 cgcgagctgc catcgtgggc ggagcttatt gaggctattc gtgaaggcgt ggaatggtac
    28441 aacaaccgcc cgcattctga gctgccgatg aaaccgaacg gacggcatta cagcccgaca
    28501 gagttcagaa aaaaacgcca ggcagaagaa gacaccgaaa ttgagtggct gagtgatctt
    28561 gagctgcgcg acatgttccg cccaatggtg gaacgaccgg taagacgctg cgaaattcag
    28621 tggctgaaca atatttacta cgcgccggag ttgcgtgacg aacacggtcg caaagtgctt
    28681 atcagttatg acattcatga cgccgagcga attacggtac gtcgcaagga cggcagcttt
    28741 atctgtgagg cgatatggaa cggcaataaa cgcgcagcct ttgctgtcag tgctgaatat
    28801 cacaaacaac agcagcgtat taaaggaatg cgcaaacgcg ctgaggaaaa aattcgtgat
    28861 gctgaagatg aaggtattca gattctggaa cacaaacagg ctgaaccctg gctgagtaat
    28921 gtttatcgcc cggttggtaa tgtggtggca gttcagcaac cggaatatga agaagagcat
    28981 gacgaagaat ttgaacgcga tttccggctg gggatgcaaa aactttttgc catgcaggaa
    29041 gaagatgatc cgctggcctg aataaaaaaa cagtccgcgg gaacggactg ttaaataaag
    29101 gaaacaaagc tgatttgaaa taaccgagga aatactatga ctgatattga tga
//
LOCUS       DAJTUI010000045        29021 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTUI010000045 DAJTUI010000000
VERSION     DAJTUI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.045"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            87..1118
                     /gene="mdtN"
                     /locus_tag="OO464_002142"
     CDS             87..1118
                     /gene="mdtN"
                     /locus_tag="OO464_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6227286.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1118..3160
                     /gene="mdtO"
                     /locus_tag="OO464_002143"
     CDS             1118..3160
                     /gene="mdtO"
                     /locus_tag="OO464_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR6227287.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3157..4623
                     /gene="mdtP"
                     /locus_tag="OO464_002144"
     CDS             3157..4623
                     /gene="mdtP"
                     /locus_tag="OO464_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6227288.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4821..5060
                     /locus_tag="OO464_002145"
     CDS             4821..5060
                     /locus_tag="OO464_002145"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227289.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5070..5240
                     /locus_tag="OO464_002146"
     CDS             5070..5240
                     /locus_tag="OO464_002146"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6227290.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5289..6701
                     /gene="fdhF"
                     /locus_tag="OO464_002147"
     CDS             5289..6701
                     /gene="fdhF"
                     /locus_tag="OO464_002147"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR6227291.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6702..6968
                     /locus_tag="OO464_002148"
     CDS             6702..6968
                     /locus_tag="OO464_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR6227292.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7062..7751
                     /locus_tag="OO464_002149"
     CDS             7062..7751
                     /locus_tag="OO464_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6227293.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7829..9142)
                     /gene="gltP"
                     /locus_tag="OO464_002150"
     CDS             complement(7829..9142)
                     /gene="gltP"
                     /locus_tag="OO464_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6227294.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9484..10080)
                     /gene="nrfG"
                     /locus_tag="OO464_002151"
     CDS             complement(9484..10080)
                     /gene="nrfG"
                     /locus_tag="OO464_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6227295.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10077..10460)
                     /gene="nrfF"
                     /locus_tag="OO464_002152"
     CDS             complement(10077..10460)
                     /gene="nrfF"
                     /locus_tag="OO464_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6227296.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10453..12171)
                     /locus_tag="OO464_002153"
     CDS             complement(10453..12171)
                     /locus_tag="OO464_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6227297.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12191..13147)
                     /gene="nrfD"
                     /locus_tag="OO464_002154"
     CDS             complement(12191..13147)
                     /gene="nrfD"
                     /locus_tag="OO464_002154"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6227298.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13144..13815)
                     /gene="nrfC"
                     /locus_tag="OO464_002155"
     CDS             complement(13144..13815)
                     /gene="nrfC"
                     /locus_tag="OO464_002155"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6227299.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13812..14378)
                     /gene="nrfB"
                     /locus_tag="OO464_002156"
     CDS             complement(13812..14378)
                     /gene="nrfB"
                     /locus_tag="OO464_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6227300.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14423..15859)
                     /gene="nrfA"
                     /locus_tag="OO464_002157"
     CDS             complement(14423..15859)
                     /gene="nrfA"
                     /locus_tag="OO464_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6227301.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16252..18210
                     /gene="acs"
                     /locus_tag="OO464_002158"
                     /pseudo
     CDS             16252..18210
                     /gene="acs"
                     /locus_tag="OO464_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18410..18724
                     /locus_tag="OO464_002159"
     CDS             18410..18724
                     /locus_tag="OO464_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR6227302.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18721..20370
                     /gene="actP"
                     /locus_tag="OO464_002160"
     CDS             18721..20370
                     /gene="actP"
                     /locus_tag="OO464_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR6227303.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20548..21840
                     /locus_tag="OO464_002161"
     CDS             20548..21840
                     /locus_tag="OO464_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6227304.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(21994..23643)
                     /locus_tag="OO464_002162"
     CDS             complement(21994..23643)
                     /locus_tag="OO464_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6227305.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23794..25143)
                     /gene="ghxP"
                     /locus_tag="OO464_002163"
                     /gene_synonym="yjcD"
     CDS             complement(23794..25143)
                     /gene="ghxP"
                     /locus_tag="OO464_002163"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6227306.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25849..26313)
                     /gene="soxR"
                     /locus_tag="OO464_002164"
     CDS             complement(25849..26313)
                     /gene="soxR"
                     /locus_tag="OO464_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6227307.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26399..26722
                     /gene="soxS"
                     /locus_tag="OO464_002165"
     CDS             26399..26722
                     /gene="soxS"
                     /locus_tag="OO464_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6227308.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26725..28302)
                     /gene="pdeC"
                     /locus_tag="OO464_002166"
                     /pseudo
     CDS             complement(26725..28302)
                     /gene="pdeC"
                     /locus_tag="OO464_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28731..29012
                     /locus_tag="OO464_002167"
     CDS             28731..29012
                     /locus_tag="OO464_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR6227309.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7277 a         7771 c         7347 g         6626 t
ORIGIN      
        1 ttgtcggaat tatttatacc gccctttttg ctctctacgc catgctgttc tggctggcat
       61 ttttctaatc tatttgagac gcgctcatgg aaagtacgcc gaaaaaagca cctcgcagta
      121 aattccctgc tctgttagtg gttgcgttgg cgctggttgc ccttgttttc gttatctggc
      181 gcgtagacag tgcgccatca actaatgacg cttacgcgtc agcagatacc attgatgtgg
      241 tgccggaagt cagcggtcgc attgtagaac tggcggtcac cgacaaccag gcggtgaaac
      301 agggcgattt gctgttccgc atcgacccgc gcccgtacga agccaacctg gcgaaagccg
      361 aagcctccct cgcggcgctg gataagcaaa ttatgctcac ccagcgtagc gttgacgccc
      421 aacagtttgg tgccgactcg gttaatgcca cggtagaaaa agcccgtgcc gccgcgaaac
      481 aggccacaga tacattacgc cgcaccgagc cattactgaa agagggtttt gtctcagcag
      541 aagacgttga ccgtgcaaga acggcgcagc gcgccgcaga agcggatctt aatgccgtat
      601 tgttacaggc gcagtcagcc gccagcgccg tcagcggcgt cgatgcgctg gtggcgcaac
      661 gtgcggcggt cgaagcggat attgccctga ccaaactaca tctggaaatg acgaccgttc
      721 gcgcgccatt tgatggccgg atcatttccc tcaaaacctc cgtcgggcaa tttgcttctg
      781 ccatgcgccc tatttttacc ctcatcgaca ctcgtcactg gtacgtgatc gccaacttcc
      841 gcgaaaccga tctgaaaaat attcgctcag gtacacccgc aacgattcgc ctgatgagtg
      901 acagcggcaa aaccttcgag ggtaaagtgg attcgattgg ctacggcgtg ctaccggatg
      961 acggcggcct ggtgctgggc ggcctgccaa aagtgtctcg ttctattaac tgggtccgcg
     1021 ttgcccagcg ttttccggtc aaaatcatgg tcgataaacc tgacccggaa atgttccgca
     1081 tcggcgcttc ggcagtcgct aatcttgagc cgcaataatg agcgcgctca actccctgcc
     1141 attaccggtg gtcaggctgc tggcgttctt tcatgaagag ttaagcgagc ggcgacccgg
     1201 ccgcgtgccg cagatcgtgc aactctgggt aggctgcctg ctggtgattc tgatctcgat
     1261 gacttttgag atcccttttg tggcgttatc gctggcagtg ctgttttacg gtattcagtc
     1321 gaacgcgttt tacaccaaat ttgtggcgat cttgtttgtg gttgccacgg tgctggagat
     1381 cggcagcctg tttttgatct acaaatggtc atacggcgaa ccgttgatcc gattgatcat
     1441 cgccgggccg atcctgatgg gctgcatgtt tttgatgcgc acccatcggc tgggactggt
     1501 ctttttcgct gtcgccattt acgggcaaac cttccccgcc atgctcgact atccggaagt
     1561 agtcgtgcgc ttaacgctgt ggtgtatcgt tgttggcctc tatccaacct tgctgatgac
     1621 gttaatcggc gtgctgtggt ttcccaatcg tgccattacg caaatgcatc aagcgcttaa
     1681 tgatcggctt gatgatgcca ttagccacct gacggacagc ctcgcaccgc tacccgaaac
     1741 gcggattgaa agagaggcgc tggcgctgca aaaactgaat gtcttttgtc tcgcggacga
     1801 tgccaactgg cgaactcaaa gcgcatggtg gcaaagctgc gtggcaacgg taacctacat
     1861 ttactcgacg ctgaatcgct acgatcccac ctcttttgct gattctcagg caattattga
     1921 atttcggcaa aaattagcct cagaaatcaa caagctgcag catgccgttg ctgaaggtca
     1981 gtgctggcaa agcgactggc ggctcagtga aagtgaagcg gtggcggcac gggaatgtaa
     2041 cctggagaat atctgccaga cgttgttaca actgggccag atgaacccga atacgccgcc
     2101 aacgcccgcc gccaaaccgc catcaatggt cgccgatgct tttaccaatc cagactatat
     2161 acgctacgcg gtaaaaacat tgctcgcctg tttgatctgt tacacctttt acagcggcgt
     2221 ggactgggaa ggcattcaca cctgtatgct gacctgcgtg atcgtcgcta atccaaatgt
     2281 cggttcgtcg taccagaaga tggtgctgcg ttttggcggg gccttttgcg gcgcgattct
     2341 ggcgctgtta ttcacgctac tggtcatgcc ctggctggac aatattgtcg aattgctgtt
     2401 tgtgctggca ccgattttcc tgttgggcgc atggattgcc accagctctg aacgctcttc
     2461 ttatatcggc acacagatgg tggtcacctt cgcgctcgcc acgctcgaaa acgtttttgg
     2521 cccagtgtac gacctggtgg aaattcgcga tcgcgccctg ggtatcatca ttggtaccgt
     2581 ggtgtccgcg atgatttaca cctttgtctg gcctgaaagt gaagcgcgca cgctgccgca
     2641 aaaactggct ggcgcgctgg gtatgttaag taaagtaatg cggatcccac gccagcagga
     2701 agtcacggct ctgcgcactt atctgcaaat tcgtatcggt ctgcatgcgg cgtttaatgc
     2761 ctgtgaagag atgtgccaac gcgtggcgct ggagcgtcaa ctggacagcg aagaacgcgc
     2821 cttactgatt gaacgttcgc aaacggttat tcgtcagggc cgcgatatcc ttcacgcctg
     2881 ggatgccacc tggaattcgg cgcaggcgct ggataacgca ctacagccgg acagagcagg
     2941 tcagtttgcc gacgccctgg agaaatacgc tgccggtctg gcaaccgcac tcagccgttc
     3001 tcctcaaata acgcttgaag agacacccac ctcgcaggcc atcctgccca ccttattaaa
     3061 acaggagcaa cacgtctgcc agcttttcgc ccgcttgcca gactggacag ccccggcatt
     3121 aacgcccgcc acggaacagg cacaaggagc cacgcaatga tcaatcgtca actttcacgt
     3181 ctgctgttgt gcagcattct cggcagcacg acgctgattt ccggctgtgc cctggtacgt
     3241 aaggattctg cgcctcatca acagctcaaa ccggaacaaa tcaaactggc cgatgatatt
     3301 catcttgcca gctccggctg gccgcaggcg cagtggtgga aacaactcaa tgacccgcag
     3361 ttggatgccc tgatccaacg gacgctaagt ggttcacaca ccctcgccga agcgaaactg
     3421 cgggaagaaa aagcgcaatc gcaggccgat ttgttagatg ccggttcaca gttgcaggtg
     3481 gcagcgttag ggatgcttaa ccgccaacgt gtctcggcga acggcttttt aagcccttat
     3541 gcgatggatg cgcccgcact ggggatggac gggccgtact atacggaagc cacagtaggt
     3601 ttgtttgccg gactggatct tgatttgtgg ggtgtgcatc gctcagcggt tgccgccgcc
     3661 attggcgcgc ataatgccgc gctggcagaa accgcagcag tagagctatc gctgaccacg
     3721 ggcgtagcgc agctttatta cagtatgcag gccagctatc agatgctcga tctgttagaa
     3781 caaactcgcg atgtgattga ttacgcggtg aaagcgcacc aaagtaaagt ggcgcacggt
     3841 ctggaagcac aagtgccttt ccacggcgcg cgggcacaga ttctggcggt cgataaacaa
     3901 attgctgccg tcaaagggca aatcaccgaa acgagagaat ctctgcgcgc attgattggc
     3961 gcgggagcca gcgatatgcc ggagatcaaa cctgtggcat taccacgagt ccagaccggc
     4021 attccggcga cactctctta tgagttgctc gccagacgcc cggatctgca agccatgcgc
     4081 tggtatgttc aggcgtcatt agatcaggtg gattccgcgc gggcgttgtt ctatccgagc
     4141 tttgatatca aagcgttttt cggccttgat gccatccacc tggatacctt attcaaaaaa
     4201 accagtcgcc agttcaactt catcccgggt ctgaaattgc cgctgtttga cggtggacgg
     4261 ttgaatgcca atctcgaagg cacgcgcgcc gccagcaaca tgatgattga acgttacaac
     4321 cagtcagtac tgaacgcggt gcgtgacgtt gccgtcaacg gcacgcgtct gcaaacgctc
     4381 aacgacgagc gagaaatgca ggctgaacac gtggaagcaa cgcgcttcac ccagcgcgct
     4441 gccgaggccg cctatcagcg cggcttaacc agccgcttac aggccactga agcccggttg
     4501 ccagtacttg ccgaagagat gtcattactg atgctggaca gccgccgggt gatccaaagc
     4561 attcagttga tgaaatcgct gggcggcggg tatcaggcgg ctcccgtcgt cgagaaaaaa
     4621 taaaatgtct gccgcgtgat ggctgtcacg cggtatttcg tttcgtcacg tcaaaactga
     4681 cgacagcctg tttttcgtca gagttttgaa taaatagtgc ccgtaatatc agggaatgac
     4741 cccaaataaa atgtggcata aaagatgcat actgtagtcg agagcgcgta tgcgtgattt
     4801 gattaactgg agcgagaccg atgaaaaaag tcgtcacggt ttgcccctat tgcgcatcag
     4861 gttgcaaaat caacctggtc gtcgataacg gcaaaatcgt ccgggcggag gcagcgcagg
     4921 ggaaaaccaa ccagggtacc ctgtgtctga agggttatta tggctgggat ttcattaacg
     4981 atacccagat cctgaccccg cgcctgaaaa cccccatgat ccgtcgccag cgtggcggca
     5041 aactcgaacc tgtttcctga gatgaggcac tgaattacgt tgccgagcgc ctgagcgcca
     5101 tcaaagagaa gtacggtccg gatgccatcc agacgaccgg ctcctcgcgt ggtacgggta
     5161 acgaaaccaa ctatgtaatg caaaaatttg cgcgcgccgt tattggtacc aataacgttg
     5221 actgctgcgc tcgtgtctga cacggcccat cggttgcagg tctgcaccaa tcggtcggta
     5281 atggcgcaat gagcaatgct attaacgaaa ttgataatac cgatttagtg ttcgttttcg
     5341 ggtacaaccc ggcggattcc cacccaatcg tggcgaatca cgtaattaac gctaaacgta
     5401 acggggcgaa aattatcgtc tgcgatccgc gcaaaattga aaccgcgcgc attgctgaca
     5461 tgcacattgc actgaaaaac ggctcgaaca tcgcgctgtt gaatgcgatg ggccatgtca
     5521 ttattgaaga aaatctgtac gacaaagcgt tcgtcgcttc ccgtacagaa ggctttgaag
     5581 agtatcgtaa aatcgttgaa ggctacacgc cggagtcggt tgaagatatc accggtgtca
     5641 gcgccagtga gattcgtcag gcggcacgga tgtatgccca ggcgaaaagc gccgccatcc
     5701 tgtggggcat gggtgtaacc cagttctacc agggcgtgga aaccgtgcgt tctctgacca
     5761 gcctcgcgat gctgaccagt aacctcggta agccgcatgc gggtgtaaac ccggttcgtg
     5821 gtcagaacaa cgtgcagggt gcctgcgata tgggcgcgct gccggatacg tatccgggat
     5881 accagtacgt gaaagatccg gctaaccgcg aaaaattcgc caaagcctgg ggcgtggaaa
     5941 gcctgcctgc tcataccggc tatcgcatca gcgagctgcc gcaccgcgca gcgcatggcg
     6001 aagtgcgtgc cgcgtacatt atgggcgaag atccgctgca aactgatgcg gagctgtcgg
     6061 cagtacgtaa agcctttgaa gatctggaac tggtcatcgt tcaggacatc tttataacca
     6121 aaaccgcgtc ggcggcggat gtcattttgc cgtcaacgtc gtggggcgag catgaaggcg
     6181 tgtttactgc ggctgaccgt ggcttccagc gtttcttcaa agcagttgaa ccgaaatggg
     6241 atctgaaaac ggactggcaa atcatcagtg aaatcgccac ccgtatgggt tatccgatgc
     6301 actacaacaa cacccaggag atctgggatg agttgcgtca tctgtgcccg gatttctacg
     6361 gtgcgactta cgagaaaatg ggcgaactgg gcttcattca gtggccttgc cgcgatactt
     6421 cagatgccga tcaggggact tcttatctgt ttaaagagaa gtttgatacc ccgaacggtc
     6481 tggcgcagtt cttcacctgc gactgggtag cgccaatcga caaactcacc gacgagtacc
     6541 cgatggtact gtcaacggtg cgtgaagttg gtcactactc ttgccgttcg atgaccggta
     6601 actgtgcggc actggcggcg ctggctgatg aacctggcta cgcacaaatc aataccgaag
     6661 acgccaaacg tctgggtatt gaagatgagg cattggtttg agtgcactcg cgtaaaggca
     6721 aaattatcac ccgtgcgcag gtcagcgatc gtccgaacaa aggggcgatt tacatgacct
     6781 accagtggtg gattggtgcc tgtaacgagc tggttaccga aagcttaagc ccgattacga
     6841 aaacgccgga atacaaatac tgcgccgtgc gcgtcgagcc gatctccgat cagcgcgccg
     6901 ccgagcagta cgtgattgac gagtacaaca agttgaaaac tcgcctgcgc gaagcggcac
     6961 tggcgtaata cagtcctttc tacagcctcc tttcggaggc tgttttttta tccattcgaa
     7021 ctctttatac tggttactcc ctacccaatc gtattatcaa aatgaaaaaa attatcgcat
     7081 tgatgttgtt tttgacattc tttgcccacg cgaacgactc cgagcctggc agccagtatt
     7141 taaaggcagc agaggccggg gaccgacgtg cacaatattt tcttgccgac agctggttta
     7201 gctccggcga tttgagcaaa gccgaatatt gggcacagaa agccgccgac agcggtgatg
     7261 ctgatgcctg cgcgctgttg gcgcagatca aaatcaccaa tccggtcagt ctggactatc
     7321 cacaagcaaa agttcttgca gagaaagcgg cgcaagcggg cagtaaagaa ggtgaagtaa
     7381 cgctggcgca tattctggta aatactcaag cgggtaaacc ggattatcca aaggcgattt
     7441 cgctgttaga aaacgcctcg gaagatctgg agaatgactc cgccgtcgat gcccaaatgc
     7501 tgcttggttt gatttacgcc aacggcgtgg gcattaaggc cgacgatgac aaggcaacct
     7561 ggtatttcaa acgcagctct gcaatttccc gaaccggtta ttccgagtac tgggcgggaa
     7621 tgatgttctt aaacggtgag gaaggtttta tcgagaagaa caaacaaaag gcgctgcact
     7681 ggttgaacct gagctgtatg gaagggtttg ataccgggtg tgaagagttt gaaaaattaa
     7741 ccaacggtta aggtaggcct gataagacgc ggcaagcgtc gcatcaggca tccacacatt
     7801 gccgggggat atccccggca atcttcaatt attgatccgc agttttatca aatttgccca
     7861 gcacttcacg ctcataagcc agcgcctttt tgcggtcaaa tttgtgttcc cacttggcaa
     7921 tcaccagcac cgccagcgca ttacccacca cgttcagcgc agtacgcgcc atgtcgagga
     7981 tacggtcaac accagcaata aacgccagac cttccagcgg aatacctacg ctacccagcg
     8041 ttgccagcaa caccacaaac gacacgccag ccacgccagc aatccctttc gaggtcacca
     8101 tcagcgtcag caccagaatg atttcctgcc agatggacag gtcaatgcca tacagctgag
     8161 cgatgaaaat agcggcaata ctttgataca gcgtcgaacc atcaaggtta aaagagtaac
     8221 cggtcggcac cacgaagctg gtgatcgacg ccggtgctcc gtaggcttcc atcttctcaa
     8281 taattcgcgg cagtacgctt tcagagctgg cagtggagta cgccagaatc agctcatctt
     8341 tcagaatacg aatcaggatc cagacgctta acccgcacag acgtgccaca attcccagca
     8401 ctaccagcgc gaagaacaga atggcgaaat gcaccagcag caccagtttc gccagtggcc
     8461 acagagacga gaaaccaaag ttagccaccg tcaccgcaat cagcgcaaac acacccaccg
     8521 gtgcataacg catcaccatg tgagtcactt taaacatggt ttcagagatg gagcggaaca
     8581 cggtcaccag cggttcacga tgcgtcgcgg gcagggaaga aagccccaga ccaaacagca
     8641 ccgagaaaaa gatgatcggc agcatttcgc ctttcgccat cgacgccaca atgttcgtcg
     8701 gcaccagcga caaaatcgtg cccataatgc cgtgggaact gctttgtacc gcttccgtag
     8761 tgctctgata tttcgagata tcgacggtcg ccagctgcga catatccacc ccggcaccgg
     8821 gctggaagac gttcgccaga gtgatcccca aaatgatggc gacggtggtg atcacctcga
     8881 agtaaataat ggttttcgcg ccaatacgcc cgagctgttt ggcatcacca acacccgcga
     8941 tacccaccac cagcgtggag atcacaatcg gcacaacaat cattttgatc agatggatga
     9001 agatatcacc cgccggagag agcaaattga cgaccagcca gtcgcggctg tcgctatggt
     9061 agtgcaggta gcttcccagg agaatgccca gcaccatagc aaacagaatc tgccaggcca
     9121 ggctgaattt tatatttttc atcagaataa cttcctcaat ggagcgccgt tccccgttca
     9181 gaaacgcgag aacgtgacat actgccaggg catgaattgt gttgcgttgg tttgtagatt
     9241 tttttaacgc ttatgatcag tatcatttgc gcggtatgtt gcgcaaccct ttaacaacag
     9301 agcttttcgc tgcattaatg cataataacg cgagtttatg gcaggattaa tgaataacca
     9361 tttgttatgg aaagattttt atttgtttaa tctgcaataa tactgattgg tgattatttt
     9421 tggcatatgt tcattcgatt attttttatg cagctcaatg acataatctt catcaccaga
     9481 tgattaatcc gatctccgct gcaacagttt tgccatatta atcgactcga ccagctgtgt
     9541 ccggttgatc cggggtgagt tgagatccat cactttttgc catagttcga tggcttgcgc
     9601 gtaattcgcc tgcataaacg catcggaagc cagcagcatc agagcggtga tttcgttaga
     9661 gtccagcgcg agggctttgt cgatcattgc gcgagtctgg gcggtcatat gctggctggc
     9721 ctggtaataa agcaccgtcg ccagcgccgc atacagttca gcgttctcac cacgtagttg
     9781 caacgcctga cgatacgcca gcagcgaatt gctgtaatcg ttttgccaca gatagtactc
     9841 gcccagtaac gcccattgtt cgctgttttg tggattagcg cggattttat cctgcaatgc
     9901 ctgaagctgc gcttccgggg tttgctggct ggcaaactga tgtagcggat cgcgctgacg
     9961 ctgatactcc gcacgtaccg cctgccattt tgggcttaac agataactgc cgacacagag
    10021 gaataccatc aatatcgtga gtgttgtcag cattttcacc ggtatttgag gctgtttcat
    10081 cgcttagccc tcactcgcca gaggatcagt gccatcagca ataacaacac cactggcagc
    10141 gcccacaaca ccagcgtctg acccgttaac ggtgggttat agcgaacaaa atctccgtag
    10201 cgttcggtca tccagccaat gatttcgact tcgttcttcc cctccgacac catgctataa
    10261 acctgatggc gcatactgac cgccaccggc gcattggatt cgagtaagtt ttgattctgg
    10321 cactgcggac aacgtaactg gctggcaata tttaacgcct gttgctgttg ttgcggattg
    10381 gcgaattgcc aggtgtctac aacctgagcg cgcgcaaaac aggtaaataa cagcagtagc
    10441 gtgagaagcc ctttattcat cgcgcttctt cccccgccat ccgcttaaca atgcgcccgc
    10501 aatcatcaac aatcctcccc cccagatcca gcgcacaccg ctttgtacat acaaacgaaa
    10561 agcgtaacga tccgctctgg ttttttcacc catgaccgca taccagtcat ggatgccgtt
    10621 ccagcgaatt gacggttcca tcatttgctg acggcgtgct tcataaaaac gccgctccgg
    10681 cgttaattca ccaatgcgtt gctgatggtc aaacagcgcc actatcgctt tttcgctggt
    10741 gtaattgcct ctggcttgca gatcgagtcg ctcaaaacgg aaggtgtatc ctgccagcgt
    10801 cacctgctga cccggctgta aattcaggct gatctcctga cgactgacac tggaaaccac
    10861 gatccccgcg gcaaataaca gcacgccagc atgagctacc agcgccggaa gctgcacgcg
    10921 tttgccagag acaaacgtcg ccagcacaat caccaccagc atcaacagac caaacggtaa
    10981 cgtcgcgcgg ttaaaatacg gcgcgccaac ggagaggcgt ccccagccca gcaggccgta
    11041 aatcatcgga taaagcgttc ccaccagcac gatcagtagc actgcgcaaa acagcaacag
    11101 cgtagcgagg attaacattt cccgcgataa ccccgaaaaa cgcaccgccg ggccaccgtc
    11161 ccgcgctcgc cagccataca gagccaaaga cgcaaggcta atcagtgcaa acaggctgaa
    11221 caacggcacg gcgcggacgt tatccagtgc gaacgcatga accgaaacca gaatgccaga
    11281 acggacaatt aaggtgccca gcagcgacag catcagagta actatcgcca gcaacagcga
    11341 ccagtggcgg aaaatccccc gctggcgtgt cagggacaaa ctgtgcagca gcgcagtggc
    11401 agaaagccag ggtaataaag aggcgttttc caccggatcc cagaaccacc agccgcccca
    11461 gccgagttcg cagtaggccc accaggaacc gaggatgatc cccgccgtta atgcactcca
    11521 gccaggtaac gcccagcgcc agcaaattcg ggcgctggcc gcatcaaact cgccgcgcag
    11581 tagactcgcc agcgccacgc tcgccgctac catcaaaccg ccatagccaa ggtaaagcag
    11641 cggtggatga aagataagac cgggatgttg cagcatcgga ttgagatcgc ggccttcgat
    11701 tgctggtgga aatatccgca cgaagggatc ggaccacagc accacaaaca gtagcagtgc
    11761 ggcgagcatg agagataaaa cggctagcgt cagcggaaat agcggatcgc tttgctgccg
    11821 ataatgccag gcaaacagcg cgctccaggc ggaaagcagc agcacccaaa gcagcagcga
    11881 accttcatga ccgccccaca ccgccgccag tttgagttcc cacgacagca ggctgtagct
    11941 atgttgggcg acataaatga ccgagaaatc gctgatgaga aaacaatacg tcagtacgcc
    12001 aaaggcgagc agcaggagcg cgaactgcgc cagaatgccg atgcaagtga gtcgcatcat
    12061 ggcaggccag cgcaaccgca ctcccgcaaa ggccgtcaac ggggtcaaca cgttgacccc
    12121 gagacttaac aacaacgcca gaaaaccgac ttcaggaagg aatacgtcca gaaaccacct
    12181 cttttctggc ttacgccacc gtcaactgtc cggcgtagag aatgaaaaag cgcagcatca
    12241 acacgccgac cagactggcc ccacacgccg ccagcacggc aggaatgccg gaactgcgat
    12301 tgacccacgg tttgagcaac attggcacaa tcagccccag cccggcgaca ccaagccaga
    12361 accaccacgt ccagaaaccg ccacctaatg ccgccaccag cgcacgaact ttaccgtcat
    12421 cgcccagcgc cagaccgaca aaaaacgcca ccagcaggaa gatttcaccc cataccacgg
    12481 ggatttccat ccggtgaaca aagtgcgctt ccgtggaatg cgggttactg cgttggcgta
    12541 tcgccatggc gatcagcgcc accgccgcac cggacgagat gccggagaag aggagcagca
    12601 ccggcaggct cgggttattg aggaacggat acgatttcag cgccgacagc agaaagccgg
    12661 tataagcccc cagcagcacc gccaacacca gcatcagcgt ttccagtccg cgatgaacgg
    12721 gcgtcagcag gctcagaacc ttttgcacga tccccagctt cggcaaccag cgttgttgca
    12781 gggcaagcaa atcatgttca aagattttcg ccagccacag caccagcacc accatgtaga
    12841 gctgaaacag catcaccccc atcgacatca ccgaggtaaa actgtagtgg aacatcagct
    12901 tccagaaggt ccacggtctt gtcaggtgga agacgaggat caacagaccg aggatcaccg
    12961 cgcccggccc gacaatcagc gtggtgcgca gcaacgtact gtctgcaccg cccgcctgcg
    13021 ggtagaagcg acgtaacagc acggccagcg tcaccagacc ggcagaaata ccaatcaaaa
    13081 acaggtagat ggcaatcggc cagtcccaca ccagcgattc aaaatgaaat gcggaagtct
    13141 gcgtcattgg ctcacctccc cgtatttaaa cggtacgcgg tacagcttcg gtttggttcc
    13201 cagcgccagc ttgtagcggt aagtaggctt ctggcgcagc agttgcgaaa tctcactgtt
    13261 aggatcgtcc agattgccaa acgtcagcgc cttcgtcggg caagcttcaa cgcacgcggg
    13321 aagcttaccg gcctgcaaat tggtcttacg acagaaatcg catttgtccg ccgtcttcgt
    13381 gaccggatgg ataaagcgca cgcggtacgg acaggcggcg atgcagtact gacaaccgac
    13441 gcagagatca ggattaacat cgacaatccc gctagcggca tcacgaaaag acgcaccggt
    13501 cgggcagacg tcaacgcacg gcgcatgatc gcagtgctgg caagacttac ggaagaagcg
    13561 atatttcaca tcaggaaatt cgccctgcgg ctcgctacga ataatcgtca ggcgcgagac
    13621 gccttccggc accttgttca cttcccgaca agcatccata caggcggtac agccgatgca
    13681 taacgactcg tcatgcacca tgccgtaacg caccccattg atattcaacg ttttcgccac
    13741 cacgcgccct gcggtgccgc tgacggctgc cagcacgccg acgccggtga gaaactggcg
    13801 acgagaccag gtcatggctg ctccttaagc aacggaacag acgccgggtt aaagttcgga
    13861 ttggtgcgct gatcgctgtg gcaatcgacg caaatcttaa tccgtccttt gtcacttaac
    13921 gtctgcatcg tatcttgctg tggatgcagg gaatggcagc tggcgcacgc cactttggtt
    13981 acgtggacat cgtgcggcca gaacgctttt tgcaactgtt ccggcagatg acaggacata
    14041 cagacgctgt tctgctctcc gaccttatac atcggctcgt taaagcgcat cacatctttc
    14101 accccttcgc ggtgttgcgg tgatgcttgg ccgtgacagt tggtgcaggt gaccggcaat
    14161 ttgttattcg ggttgataac ggaagcatgt ttgccatgca taccttcggt atctggttta
    14221 tgacagtcca gacaggcggc atccgggtta cgctgctggg taacttcgta gcgatcacca
    14281 gatgcctgca ccgcaggggt agcggtaatt ccgttcaggc tccacaacag gcccgacgcc
    14341 agcaccccag cagttaataa cgaacgtaat acgctcatat tcactccatt gtgttttccg
    14401 ccccttgcga ggcggaacgg ggttattggc ttaacagacc gtttttacgt gcctgctctt
    14461 cccactgcgg gatcaccgtt ttgatgaagt cctgcttctc ggccttgatt tgttccatgt
    14521 tcaggccaat ggcctgctgg gctttctctt tggtcgagat atccgggatc tggatttcat
    14581 gggtgatacc tttggtcgcc agcaggcgcg ccagtttggt gcgcgcatcc gccgctttat
    14641 ccatcgccgt accgagcatc cgtagacctt cttccggtgc gtgcatatga ataccgtggg
    14701 aagcaatcgc cagatcccaa cgccactggg catgacggat atcgtcctga atcggcttca
    14761 tttcggcttc cgtcgcgcct gcatccagcg ccgctttcgc ttcgaagtga gcgtgaacca
    14821 gttgatcttc aaccttgatt ttcaggtcgt taatcgattg cttacgttcc gcgaccactt
    14881 tttgcaaggc agctttatcc tgggtatggc agttcgcaca agtctgggcg aagttatcaa
    14941 acggattacc aattttatgg tcggtgtaga gtttgccttc ggcgttctgc acttttggca
    15001 tatggcagtc gatacaggtc acgttgtttt taccgtgaat gcccgctgtc caggtttcat
    15061 attccgggtg ctgcgctttc agcattggcg ttttcgacag ggagttagtc cagtcagaga
    15121 aggcaatttt gtcgtaatac tgctccatat tttcgacttt catgccgtca tcccacggga
    15181 atttaaccgc tttgtttttg ccgtcgaagt aatactccac atggcactga ccgcaaacca
    15241 tcgattgctg gtcgaaacgt ccggctttct caaaaggttt accaatggct tccatcgcgc
    15301 gagccgcata cggacgggaa agggttaact ccggtttgcc tttggcgaac tctggtgagg
    15361 cggtgttatg gcagtcggca caacctaagt tgttgacgat ttccgggccg ccgcgcgccc
    15421 atttaccgtg gaagtagcca tcttcgccgt ctttctggat cagacgcgcc acatccgggc
    15481 ttttacaact ccagcacgcc atcggtagcg gaccatcttc agcgtttttc ggcgcaccgg
    15541 tacgcagggt ttcacgcaca tcggtcacag caaaggcatg tccacgaggc ttgttgtaat
    15601 cgcgcgagaa gggatacccc gcccacagga tcaccagccg tggatcttcc gccagggcgt
    15661 caacacgctc tgactgttcc gaggtggctt tccaggagag atattgatcg tgatgctgcg
    15721 gggcaaaggt ttcattcttc gcttccacag ttacaggttt tgcgggagct gccgtttgtt
    15781 cagcgtgaac agaagtgaaa aagaaaaaag gaatcaataa gctgaagata cggcgtgcgt
    15841 ttatttttat ccttgtcata ggggcttcat ccgaattgcg ccattgttgc aatggcggtt
    15901 tttattgttt ttcacgacag taaccgcacc tacactgtca tgacattgct cacccctatg
    15961 tgtaacaaat aaccacactg tgaatgttgt ctttaatcaa ttgtaagtgc atgtgaaata
    16021 ccactttaga gttagtcagt atcttcctct ttttcaacag catgcataac tgcatgttcc
    16081 tcaaagaatt aatcaacttt tgttggtgac cttcaaaaat taccctgccg tttatttgca
    16141 ccattctact tttgcgtgat ctgtcgccca aatactaaac aaaactgcca atacccctac
    16201 atttaacgct tatgccacat attattaaca tcctacaagg agaacaaaag catgagccaa
    16261 attcacaaac acaccattcc tgccaacatc gcagaccgtt gcctgataaa ccctcagcag
    16321 tacgaggcga tgtatcaaca atctattaac gtacctgata ccttctgggg cgaacaggga
    16381 aaaattctcg actggatcaa accgtaccag aaggtgaaaa atacctcctt tgcccccggt
    16441 aatgtgtcca ttaaatggta cgaggacggc acgctgaatc tggcggcaaa ctgccttgac
    16501 cgccatctgc aagaaaacgg cgatcgcacc gccatcatct gggaaggcga cgacgccagc
    16561 cagagcaaac atatcagcta taaagagctg caccgcgacg tctgccgctt cgccaatacc
    16621 ctgctcgagc tgggcattaa aaaaggtgat gtggtggcga tttatatgcc gatggtgccg
    16681 gaagccgcgg ttgcgatgct ggcctgcgcc cgcattggcg cgatgcattc ggtgattttc
    16741 ggcggcttct cgccggaagc cgttgccggg cgcattattg attccaactc acgactggtg
    16801 atcacttccg acgaaggcgt gcgcgccggg cgtagtattc cgctgaagaa aaacgttgat
    16861 gacgcactaa aaaacccgaa cgtcaccagc gtagagcatg tggtggtact gaagcgtact
    16921 ggcggaaaca tcgactggca ggaagggcgc gacctgtggt ggcacgacct ggttgagcaa
    16981 gccagcgatc agcactaggc ggaagagatg aacgccgaag atccgctgtt tattctctat
    17041 acctccggtt ctaccggaaa accaaaaggc gtactgcaca ctaccggcgg ttatctggtg
    17101 tacgcggcgc tgacctttaa atatgtcttt gattatcatc cgggtgatat ctactggtgc
    17161 accgccgatg tgggctgggt taccggacac agttatttgc tgtacggccc gctggcctgc
    17221 ggcgcaacca cgctgatgtt tgaaggcgta ccgaactggc cgacgcctgc ccgtatggcg
    17281 caggtggtgg acaagcatca ggtcaatatt ctctataccg cacccacggc gatccgcgcg
    17341 ctgatggcgg aaggcgataa agcgattgaa ggcaccgacc gttcgtcgct acgcattctc
    17401 ggttccgtgg gcgagccaat taacccggaa gcgtgggagt ggtactggaa aaaaatcggc
    17461 aacgagaaat gtccggtggt cgatacctgg tggcagaccg aaaccggcgg tttcatgatc
    17521 accccgctgc ctggcgctac cgagctgaaa gccggttcgg caacacgtcc gttcttcggc
    17581 gtgcaaccgg cgctggtcga taacgaaggt aacccgctgg aaggggctac cgaaggtagc
    17641 ctggtgatca ccgactcctg gcctggtcag gcgcgtacgc tgtttggcga tcacgaacgt
    17701 tttgagcaga cctatttttc caccttcaaa aatatgtatt tcagcggcga cggcgcgcgt
    17761 cgcgatgaag atggctatta ctggatcacc gggcgtgtgg acgacgtgct gaacgtctcc
    17821 ggtcaccgtc tggggacggc agagatagag tcggcgctgg tggcgcatcc gaagattgcc
    17881 gaagccgccg tagtaggtat tccgcacaat attaaaggtc aggcgatcta cgcctatgtc
    17941 acgcttaatc acggggagga accgtcacca gaactgtacg cagaagtccg caactgggtg
    18001 cgtaaagaga ttggcccgct ggcgacgcca gacgtgctgc actggaccga ctccctgcct
    18061 aaaacccgct ccggcaaaat tatgcgccgt attctgcgca aaattgcggc gggcgatacc
    18121 agcaacctgg gcgatacctc gacgcttgcc gatcctggcg tggtcgagaa gctgcttgaa
    18181 gagaagcagg ctatcgcgat gccatcgtaa cccacaattg ccggatgcga atcggtaacg
    18241 gtttgtaggc ctgataagac gcgacagcgt cgcatcaggc attgattgcc ggatgcggcg
    18301 taaacgcctt atccggccta cattcggcaa gggttacccg agcgttaacc ttctcccata
    18361 agggagcggg aattaaaaca atccctacat tacctctgga gaatctgtga tgaatggtac
    18421 gatttatcag cggatagaag acaatgcgca tttcagggag ttagtcgaaa aacggcaacg
    18481 gtttgccacc atcctgtcga ttattatgct ggcagtttat atcggcttta ttttactgat
    18541 cgccttcgcg cccggctggc tgggcacgcc gctgaatccg aacaccagcg tcacacgcgg
    18601 tattccaatt ggtgttggag tgattgtgat ctcctttgtt ctcaccggta tctacatctg
    18661 gcgggcgaac ggcgaattcg accgtcttaa taatgaagtc ctgcatgagg tacaagcatc
    18721 atgaaaagag ttctgacggc gcttgccgcc acactccctt tcgcagctaa cgccgcggat
    18781 gctattagcg gggccgtaga gcgccagcca acgaactggc aggcgattat tatgttcctg
    18841 attttcgtcg tgtttacgct cggcattacc tactgggcat caaaacgcgt acgttctcgt
    18901 agcgactact acaccgcagg cggcaatatc actggcttcc agaacgggct ggcgattgcc
    18961 ggggactata tgtccgccgc ctcattcttg gggatctccg cgctggtgtt tacctccggc
    19021 tatgacgggc tgatctactc gttgggcttc ctggtgggct ggccaatcat tctgtttctg
    19081 attgccgaac gtctgcgtaa cctgggacgc tacacctctg ccgatgtggc ctcttatcgt
    19141 ctgaaacaag ggccgattcg tattctttcg gcctgtggtt ctctggtagt ggtggcgctt
    19201 taccttatcg ctcagatggt aggcgcaggt aaactgatcg agctgctgtt tggccttaac
    19261 tatcacattg cggtggtgct ggtcggcgtg ctgatgatga tgtacgtcct gttcggcggc
    19321 atgctggcga ccacctgggt acaaattatc aaagctgtgc tgttgctgtt cggtgccagc
    19381 tttatggcct ttatggtgat gaaacacgtc ggctttagct tcaacaatct gttcagcgaa
    19441 gcgatggcgg tacacccgaa aggtgtcgac atcatgaaac cgggcggact ggtgaaagat
    19501 ccgatctccg cgctctctct ggggctggga ctgatgtttg gtacggcggg cttgccgcac
    19561 attctgatgc gcttctttac agtcagcgat gcccgcgaag cacgtaagag cgtgttctac
    19621 gccaccggat ttatgggcta cttctatatt ctgaccttta ttatcggctt cggcgcgatc
    19681 atgcttgttg gtgcgaatcc ggaatataaa gacgcggcgg gccatctgat tggtggtaac
    19741 aacatggcgg ccgttcacct ggcgaatgca gtgggcggca acctattcct cggttttatt
    19801 tcagcggttg ctttcgccac tatcctcgcg gtggttgcgg atctgacgct ggcgggcgca
    19861 tcggcggttt cgcatgactt gtacgctaac gtcttcaaaa aaggcgcgac cgaacgtgaa
    19921 gagctgcggg tatcaaaaat caccgtactg atcctcggcg tgattgcgat tatcctcggc
    19981 gtgctgtttg agaatcagaa catcgccttt atggtaggcc ttgcgtttgc catcgcggcg
    20041 agctgtaact tcccgatcat tctgctttcc atgtactggt cgaaactgac tacccgtggc
    20101 gcgatgctgg gcggctggct ggggctgatt accgcagtgg tgctgatgat cctcggcccg
    20161 actatttggg tacagatcct cggtcacgaa aaagccatct tcccgtatga atacccggcg
    20221 ctgttctcta tcagcgtggc attcctcggc atctggttct tctcggcaac cgataactca
    20281 gcggaagggg cgcgcgagcg tgaactgttc cgcgcgcagt ttatccgctc ccagaccggc
    20341 tttggcgttg agcaaggccg cgcacattaa tctctcccct tccccggtcg cctgaccggg
    20401 gaatacgctt cctctccaac atgcatcacc ttttcccata atattaaaca aataaactca
    20461 ttaaaaaatg agcgattttt gacagtcgta gaaaatgata atgcagagaa tatgcctttt
    20521 ctttcttgtt aattataagg atattttatg cgctataatg gtttaaataa tatgtttttc
    20581 cctctttgcc agattaacga taaccactct atcacaagtt catcacatac aaagaaaaca
    20641 aaatcagata attacagcaa acatcataaa aacacgttaa ttgacaataa agccctctct
    20701 cttttcaaaa tagatgatca tgaaaaagtg ataggcttga ttcagaaaat gaaaagaatt
    20761 tatgatagtt taccatcagg aaaaatcacg aaagaaacgg acaggaaaat acataaatat
    20821 tttatagata tagcgttata tgcaaataat aaatgtgacg atagaattac gagaagagtt
    20881 taccttagta aagaaaagga tgtatccatt aaggtggtat attttataaa taatgtcgcc
    20941 gtccataata atactatcga aattccacag atagtaaatg gtggttacga tttttcacac
    21001 cttagcctga agggtatcgt gattaaagat gaagatttat ccaattcgaa ttttgcaggt
    21061 tgcagactac aaaacgctat tttccaggac tgtaatatgt ataaaacgaa tttttattac
    21121 gccataatgg aaaaaatact ttttgataat tgtattctcg atgactcaaa tttcgctcag
    21181 ataaaaatgg ccgacggaac tctaaatgca tgctccgcta tgcatgttca attctacaat
    21241 gcagcaatga atagagccaa tattaaaaat accttccttg actattcaaa tttttatatg
    21301 gcgtacatgg ctgaggtaaa tctttataaa gtaatagcgc catatgttaa tttatttaaa
    21361 gccgacctta gtttctctaa actcgattta attaactttg aacatgctga tctgtctcgc
    21421 gtcaatctga acaaagcaat cctccagaat ataaacttaa ttgatagcaa actcttttgt
    21481 acgtggctga caaatacatt cctcgaaatg gttatatgta ccggctctaa tatggctaat
    21541 gttaatttta ataatgccaa tttaagcaac tgccatttca actgttctat tttaacaaaa
    21601 gcctgtatgt ttaatacccg tctctatcgg gttaatttcg atgaggctag cgtccaggga
    21661 atgggcattt ccattctccg tggggaggaa aatatcccca ttgatagtga taccctggta
    21721 acactacaga aattctttga agaagattgt acctctcata ctggcatgtc acaaactgag
    21781 gataatatta atgcagtcgc tatgaagatt actgcagata ttatgcaaca cgcagattga
    21841 ggttaagtcg aagaacagcc aatgaattga ctttacaaga gagataactt atttgtttta
    21901 gaatcatgaa tgttcgcccg agctaacctg ttaaaggtgc aaaagattgc acctttttca
    21961 gattacctcg ggcctggtta cgtcgctttc ggcttactga ttttcctcaa ttagcaacgc
    22021 ttcaagcaaa tcgagatcgt gcagtaattt ttgcagcgtt tcgttgctga tctcccgcgt
    22081 ggcgcgcagg tggtacagtt cagcacgttc agaacgcaat gccgccagac ggaagcgacg
    22141 ctcaaggttc tcttcctgca cggaactttc aacgtcgtta cgtccatcgg cgcgacgacg
    22201 caggttacca atgacgcgag aactgacctc cgtaagcaac tggttatcga tattctcttc
    22261 ggtatccgcc gccagacgct cctccatttt ctggatcgcc acaatcgcca cttctgccgt
    22321 tgccgctcgc gcaatacgtt cttctttcaa ttgttgcgaa tggtccgcga cttcaatgtg
    22381 ttgtagcaga ataggcaaca tcaccacgcc gacaaacagc gagaagagaa tgacgccagc
    22441 cgccaggaac accagctcat agcgcgccgg gaagacgtta ccatccggca agagcagcgg
    22501 gattgagagc acaccggcca gagtgatcgc cccacgcacc ccagcgaaag acgcgatcag
    22561 gatttctcgt gtggtccacg aaccaaactc catcggcttc ttcttcagga agcggttgct
    22621 gaactttttc atcgtccaca gccagccgaa acggaccagc atcagcgccg catatatcag
    22681 aataatatcg gtaaacagca tccagatttc gacgttaggg tcgatttctg ccgccatcag
    22741 cgacgtctcc agaatacccg gcagttgcag acctaacagc aggaacacca tgccgttaaa
    22801 gacaaattcc agcatcgccc aggtgctgtt tgcgcgcagg cgcattgcca gtggcgcacg
    22861 gcgcatcaca ccggagcggg tgatggtcat cccggcggca accgcagcga ggatgcccga
    22921 aacgccaata tgttcggcaa tcagataaga agcgaatggc agcaagaaca gcagaacgat
    22981 ctgcgtcgcg ggttcatcac cgccccagcg gctgaggaat cgcagcgagc gaccatacag
    23041 ccagctcacc acaaaaccgg caagaatacc gccaatggct actttcataa attcgaccgt
    23101 cgcaccgccg acagtgaaga tcatcgtccc catcgccact gccacggcaa acttcaacga
    23161 caccaggccg gaagcgtcat tcatcaacgc ttcgccctgc aaaatgccca tgattttttt
    23221 cgggatgcgc ccttcgccaa caatcccgga gagcgccaca gcatcggtcg gagaaagtac
    23281 cgccgccagc gcaaaggcgg ggatcagcgg aatgcccggc accacccagt aaataaggaa
    23341 gccaatgccg accacggtga ccaccaccag cgccagcgcg aggccgaaaa tctctcgacc
    23401 atgttcgaga aattcacggg tcggcgtttt ccagccatca gcgaacagca acggcgggat
    23461 aaacaagact aaaaagagtt caggatcaaa ctccacatgc aaaccaaacg tcggccacgc
    23521 cagtagcgca ccgatggcga tttgcataag cggaagcggg atctgaaagg gcatgacacg
    23581 agtgaccacc ccggacagcg agaccacgag ggtcattatc agtatggtga agaagatttc
    23641 catgcgttcc ctgtttgcga tgttttttat gatctgagaa agacgttatg cctgaattct
    23701 cattttttca gaatatcgca aaagctcaca caatgtgcgg agaaaataaa aacgggcagc
    23761 cattgccacc cgttttcaga atccgatgaa agattagata gcccagccac ccgcatagaa
    23821 ggtcaccagc gccacggcga taacgaccgt accgatgttc aacttgcgcc attcaccgga
    23881 aaccagacga ccaatcacca gagtcgcgaa gccgatcatg atgcctgtta cgatgttaca
    23941 ggtcagcacg atgaataccg ccgtaaccag acccgccatc gcatcaacaa aatcagcaaa
    24001 gtcgattttc gccacgttgc tcagcatcag caggccaacg tacatcagcg ccggagccgt
    24061 tgcatacccc ggaacgaggt aagaaagcgg agagaggaac agaatcagca ggaacagcac
    24121 gccaacggtg atagccgtca acccggtttt accgcccgcc gccgtacccg ccgcagactc
    24181 gatgtatacc gctgccggag ctgcacccac caggccagag aaaacgctgc tcatggagtc
    24241 agtggtcagt gctttcccgc cgtcgatgat ctgcccatct ttatccagca ggttcgcctg
    24301 gccggcgaca gcgcggatag ttccggtggc atcaaatact gccgtcatca ccagcgccag
    24361 aacgcttggc aggactacag gattcagcgc gcccataatg tccaggctgc caatcagtga
    24421 attgccgttt tcatcgctca atgaaggcat ggcgaaaacg ccggagaaat ggacgttagg
    24481 atcgaagatc aaaccgacaa ttgagatacc gataatggtc agcagaatgc caccagggac
    24541 tttcagtttt tccaagccga tgatcaccgc cagacctgcc agtgacataa tcaccgggaa
    24601 ggtcgcgaaa tcacccagcg caacgggcag accatcaagc gggtttttaa tcaccagacc
    24661 gacaccatta gcggcaatga gcagcaggaa cagaccgata ccgatccccg tgccgtgcgc
    24721 cacaccgtgc ggcaagttgc gcaaaatcca gctacggata cccgtggcag aaattaccgt
    24781 aaacagcaca cccatcagga acacggcacc cagcgcgaca ggtacgctaa tatgttgccc
    24841 cagcaccagg ctgaatgcgg taaacgccgt cagggagatg gcgcaaccaa tcgccaacgg
    24901 cagattagcc cacagaccca tcacgataga accgagtccg gcaaccagac aggttgcaac
    24961 gaaaactgcc gcaggcggga agcctgcttt acccaacata cctggaacga cgatgaccga
    25021 gtagaccatc gccagaaacg ttgttaaccc ggcaaccact tcctgacgga cagtgcttcc
    25081 acgttgtgaa attttaaacc aggcgtcgag tgaaccgccg gtacgcgctg atggcgtaga
    25141 cataagaaaa catcccctga gagtttaatt ttcgtcaaga tgcgtggcgg acaatcccct
    25201 gccagcgaaa cgttatcttc ctgctccagg tttgcggcaa tttggtgcgt cgcaaaaaag
    25261 caaacgttta gctccgcgca tacaaaacgg gtggtaaaaa aaagcaaacg attatccggc
    25321 gatcatagcg gctttttcaa ccgaagtttg ctgctttttt ctcttttatt gtcttgacga
    25381 tgaaaaacca ggcaattgat gcgcaaacgt tatcgtcgat gtgcaagatt gcagcgttat
    25441 cgagccgtat accgttctgg attgcgataa aaaatcgtct gagatgacaa aaatattgcg
    25501 gctaaaaagt atacttttac actggcgaag aaacgtcggt gacatacaat taaagcatca
    25561 acaccaaccg gaacctccac cacgtgctcg aatgaggtgt gttgacgtcg ggggaaaccc
    25621 tcctgtgttc cagcgggata gagagaaaga caaagaccgg aaaacaaact aaagcgccct
    25681 tgtggcgctt tagttttgtg tttgcggata attccaaaag agctcgatca gtcccctcgc
    25741 cccttcgggg agagggttag ggtgagggga acaggcagca ccagtgcaaa tatttaccct
    25801 caccccggcc ccatagttaa cgatgagtca gcttgtcctt gtggcgcttt agttttgttc
    25861 atcttccagc aagcgtgcgc cggtaccttc ttctcctaag cggtcgcccg ggttacgcaa
    25921 cgggcaatca ctgcgcgaaa ggcagccaca accaatacat ccgtccagtt cgtcacgcag
    25981 cgccactaag gtatgaatgc gccgatccaa ctcttctcgc cattgggacg aaagctgttt
    26041 ccactctttc gcacttaacg tatgcccttc tggcaacacg ccaaacgctt caccaatggt
    26101 cgccagcgga atgccaatac gctgagcgat tttgataatt gcgacatatc gcaacacatc
    26161 acgtttatat cgccgctgat tgccgctgtt acggatacta gtaatcaacc ctttgctttc
    26221 atagaaatgc agcgccgata ccgccacacc gctgcgtttc gccacttcgc caggggttag
    26281 cagcgcttta atgcggggta atttcttttc cataaatcgc tttacctcaa gttaacttga
    26341 ggaattatac tccccaacag atgaattaac gaactgaaca ctgaaaagag gcagatttat
    26401 gtcccatcag aaaattattc aggatcttat cgcatggatt gacgtgcata ttgaccagcc
    26461 gcttaacatt gatgtcgtcg caaaaaaatc aggctattca aagtggtact tgcaacggat
    26521 gttccgcacg gtgacgcatc agacgcttgg cgattacatt cgccaacgcc gcctgttact
    26581 ggccgccgtt gagttgcgca ccaccgagcg tccgattttt gatatcgcaa tggacctggg
    26641 ttatgtctcg cagcagacct tctcccgcgt tttccgtcgg cagtttgatc gcactcccag
    26701 cgattatcgc caccgcctgt agttttattg cccgcgcgtt aactcccgcg cgggtaattg
    26761 ctccatccat tgcataaaca cctgcggcgg catcgccttc gcaaaaaacc atccctggca
    26821 atagcgcacg ccgcgtttgc gcagccagtt aacctgctcc tcagtttcga cgccttcagc
    26881 gatcgttttt aaccccaggc tgtgcgccag ctcgatgatg tgttccgcaa tcaaatgact
    26941 ggttttgtgg gtggtcagcg tttcaacaaa cgatttatcg attttcaaaa tatcgacatt
    27001 caatgattta aggttatgca agttagagta gccaatacca aaatcatcaa ttgccacttc
    27061 gtaaccagcc tggcggaaag ccagaattaa tcggcgtcat tttttcgaca tcaagaaatg
    27121 catgctcagt cacttcaaat ttaatttgct gcggacgcac cgcgtattgc tctgttttct
    27181 gattgattcg cgctatcaac cgtgacgtat gaaaatcgga ggccgacagg ttaatagaaa
    27241 catagcgatc ggcatgtgtt gccaggtacg cgcccagatc gcggaagaca ttatcaataa
    27301 cataatcagt tatctgttct atcatcccct ccttttctgc cagcggaata aactctgccg
    27361 gattcattat ttgcccctgc tcaccaggcc aacgtaacaa cgcttcagcg ccgatacatt
    27421 tttctgtttt gatatcgatt attggctggt aataaagaca aagttgatgt ttttcgaggg
    27481 cgcgttgcaa tttacgtttg ggagataaat agttttgtcg aatacgtagc cagagtaata
    27541 gcagaacaag actccccaaa ataccggcgg gcaacgcaaa aataagatga ttataaaaat
    27601 gggttataag acgttgatat gaagtcgcaa caatggctgc aattgggcgt tttgtcgaat
    27661 aaattgtcgc atataaatag ccatttcttt gtacggttaa atccttcaaa tgaatcagcg
    27721 gagaaaacgt tgctgccgag gcctctttgc ttaacgagaa aaaggttttc atcaccgtat
    27781 catacacacc ccattgcaat gtcgggtcat cagacatcac ttcactccag aagagagggt
    27841 tgataaccgc cacataattt ccccgctgca tataggtcat tttatagcca gagaaaaaag
    27901 gcgtatcgcg gtaataatag atagaaacgt taggttcacg cttataatcg gccggtgcaa
    27961 tcgtatagcc gtttacaggc gctatcagcg atgagcataa aaaatggtta tcacgaattc
    28021 attaatataa agatagccac gaataatatt caacattcgc ttttgatggg ctggagtgca
    28081 aacttgcccc tgatagcgtt cagctgcatc gctcactaaa tctgcctggc gaatgaccag
    28141 ctcggattta tccagtgcca gttgagcaaa ggttcggagc tgattattca cttctgattt
    28201 tgcccaaagg aaggcaatcc atagcgaaag aatgatggga aagagaacta aaaagataat
    28261 gcccggcaac gccagtaatt ggtgtcgtgc acgatgactc attgctatca atttcctgcc
    28321 tgtttatacc cggccattgc aaggtgaaac cctggctacc gacgtcttta gataaatctt
    28381 acggtataaa tagcactttt gcctgatttg aaattgatac ctttttaatc gtcacctgga
    28441 tgaaaataat tcattgatat tcaaaattaa ttttctacag gaatacagag gcggcgggaa
    28501 gatatgccct taatgccact gaaaaaccac ataatttgta cagcaataaa agtcacggcc
    28561 taatatggct aacaaacaat caattgtgat atagttcaca aaattaatga aacaaacaga
    28621 gtgtttcatt tttgtgttct gcaagccccg cgatagccag agtgtaccac gcctcccgtg
    28681 aacaacgccg cgctgtccag ggcatcggct cttttcagag gaattgtttg atggctaccc
    28741 tcaccactgg cgtggttctt cttcgctggc aacttcttag tgccgtaatg atgtttctgg
    28801 ccagcacact caacatccgt tttcgtcggt ctgattatgt cgggcttgcg gtgatcagca
    28861 gcggtctggg cgttgtttcc gcctgttggt tcgcaatggg gttgcttggc atcacaatgg
    28921 cggacatcac cgctatctgg cacaacatcg agtcggtgat gatagaagag atgaatcaga
    28981 caccgccaca atggccaatg attttgacct gatacgtaga a
//
LOCUS       DAJTUI010000046        28815 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTUI010000046 DAJTUI010000000
VERSION     DAJTUI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28815
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.046"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO464_002168"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO464_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR6227310.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="OO464_002169"
     CDS             complement(2138..2584)
                     /locus_tag="OO464_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6227311.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="OO464_002170"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="OO464_002170"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6227312.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO464_002171"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO464_002171"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6227313.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="OO464_002172"
     CDS             complement(5347..6009)
                     /locus_tag="OO464_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6227314.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="OO464_002173"
     CDS             6182..6595
                     /locus_tag="OO464_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6227315.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO464_002174"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO464_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6227316.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO464_002175"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO464_002175"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR6227317.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="OO464_002176"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="OO464_002176"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6227318.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO464_002177"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO464_002177"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6227319.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO464_002178"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO464_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6227320.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="OO464_002179"
     tRNA            complement(9861..9948)
                     /locus_tag="OO464_002179"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="OO464_002180"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="OO464_002180"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6227321.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="OO464_002181"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="OO464_002181"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR6227322.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="OO464_002182"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="OO464_002182"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR6227323.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="OO464_002183"
     CDS             14005..14592
                     /locus_tag="OO464_002183"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6227324.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="OO464_002184"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="OO464_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6227325.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="OO464_002185"
     CDS             14984..15841
                     /locus_tag="OO464_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR6227326.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="OO464_002186"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="OO464_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6227327.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="OO464_002187"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="OO464_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6227328.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="OO464_002188"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="OO464_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6227329.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="OO464_002189"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="OO464_002189"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6227330.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO464_002190"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO464_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO464_002191"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO464_002191"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR6227331.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="OO464_002192"
     CDS             complement(22884..24023)
                     /locus_tag="OO464_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR6227332.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="OO464_002193"
     CDS             complement(24069..26165)
                     /locus_tag="OO464_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6227333.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="OO464_002194"
     CDS             complement(26165..26911)
                     /locus_tag="OO464_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6227334.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /locus_tag="OO464_002195"
     CDS             complement(26908..27552)
                     /locus_tag="OO464_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6227335.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="OO464_002196"
     CDS             complement(27659..27964)
                     /locus_tag="OO464_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227336.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO464_002197"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO464_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR6227337.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         6974 a         7299 c         7582 g         6960 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgcagccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatcc
//
LOCUS       DAJTUI010000047        28419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTUI010000047 DAJTUI010000000
VERSION     DAJTUI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.047"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            563..1333
                     /locus_tag="OO464_002198"
     CDS             563..1333
                     /locus_tag="OO464_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR6227338.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1324..1797
                     /locus_tag="OO464_002199"
     CDS             1324..1797
                     /locus_tag="OO464_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6227339.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1904..2443
                     /gene="dnaT"
                     /locus_tag="OO464_002200"
     CDS             1904..2443
                     /gene="dnaT"
                     /locus_tag="OO464_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6227340.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2446..3183
                     /gene="dnaC"
                     /locus_tag="OO464_002201"
     CDS             2446..3183
                     /gene="dnaC"
                     /locus_tag="OO464_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6227341.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3232..3726
                     /locus_tag="OO464_002202"
     CDS             3232..3726
                     /locus_tag="OO464_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6227342.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3966..6257
                     /gene="opgB"
                     /locus_tag="OO464_002203"
     CDS             3966..6257
                     /gene="opgB"
                     /locus_tag="OO464_002203"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6227343.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6395..7417)
                     /gene="lgoD"
                     /locus_tag="OO464_002204"
                     /pseudo
     CDS             complement(6395..7417)
                     /gene="lgoD"
                     /locus_tag="OO464_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7556..8470
                     /locus_tag="OO464_002205"
     CDS             7556..8470
                     /locus_tag="OO464_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6227344.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8685..10046
                     /locus_tag="OO464_002206"
     CDS             8685..10046
                     /locus_tag="OO464_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6227345.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="OO464_002207"
     CDS             complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="OO464_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR6227346.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEISSASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="OO464_002208"
     CDS             complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="OO464_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6227347.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12596..13885
                     /locus_tag="OO464_002209"
     CDS             12596..13885
                     /locus_tag="OO464_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6227348.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13882..15348
                     /gene="hpaE"
                     /locus_tag="OO464_002210"
     CDS             13882..15348
                     /gene="hpaE"
                     /locus_tag="OO464_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6227349.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15350..16201
                     /gene="hpaD"
                     /locus_tag="OO464_002211"
     CDS             15350..16201
                     /gene="hpaD"
                     /locus_tag="OO464_002211"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR6227350.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16211..16591
                     /locus_tag="OO464_002212"
     CDS             16211..16591
                     /locus_tag="OO464_002212"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6227351.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16659..17462
                     /gene="hpaH"
                     /locus_tag="OO464_002213"
     CDS             16659..17462
                     /gene="hpaH"
                     /locus_tag="OO464_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6227352.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17473..18276
                     /gene="hpaI"
                     /locus_tag="OO464_002214"
     CDS             17473..18276
                     /gene="hpaI"
                     /locus_tag="OO464_002214"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR6227353.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18298..19653
                     /gene="hpaX"
                     /locus_tag="OO464_002215"
     CDS             18298..19653
                     /gene="hpaX"
                     /locus_tag="OO464_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6227354.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19663..20553
                     /gene="hpaA"
                     /locus_tag="OO464_002216"
     CDS             19663..20553
                     /gene="hpaA"
                     /locus_tag="OO464_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR6227355.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20580..21517
                     /locus_tag="OO464_002217"
                     /pseudo
     CDS             20580..21517
                     /locus_tag="OO464_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21744..23302
                     /gene="hpaB"
                     /locus_tag="OO464_002218"
                     /pseudo
     CDS             21744..23302
                     /gene="hpaB"
                     /locus_tag="OO464_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23320..23832
                     /locus_tag="OO464_002219"
     CDS             23320..23832
                     /locus_tag="OO464_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6227356.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24225..26375
                     /gene="btsT"
                     /locus_tag="OO464_002220"
     CDS             24225..26375
                     /gene="btsT"
                     /locus_tag="OO464_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6227357.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26425..26628
                     /locus_tag="OO464_002221"
     CDS             26425..26628
                     /locus_tag="OO464_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6227358.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26639..27595
                     /gene="yjiA"
                     /locus_tag="OO464_002222"
     CDS             26639..27595
                     /gene="yjiA"
                     /locus_tag="OO464_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6227359.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27664..>28419)
                     /locus_tag="OO464_002223"
     CDS             complement(27664..>28419)
                     /locus_tag="OO464_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCR6227360.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6956 a         7425 c         7126 g         6912 t
ORIGIN      
        1 ttaaatgttt ccagcaaatt aatagattaa gcgaataaat tatccttagc attcactttc
       61 atatcagcat actcctgctg cacaacaact ccactgcatg tgtcagaagg ttagcatcaa
      121 atgataacct ggtgaaatag cctggagaca tttaaaatat ctacattaag ttgttagaaa
      181 tacttaatga aaaacagaaa tctcattcat ttaatttata attaaatgat atctcttccc
      241 gcagaatgcg ggaatcacac aatcaatatt tattcaataa aatctgatga ttttaacttg
      301 ttaacactaa aaaacgcgcc cagctatcta ttatcctgac ccgtaaaggc taaacaataa
      361 attcattata gtgataatat tcatgtgata gtttgtttgc ggcgagagat aattcgcttt
      421 ttatcaccga gcttgtccca ataaccagga ttaatcagcg caattttgat cttaacctac
      481 cgcaaaacaa ccgtgcttca tgatacaaat ggattctcat tatctgaaca atacgcagca
      541 cgtctatgac aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa cgcggctttg
      601 tatccagtgt ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct
      661 ttcctcacga ctgggtcggg cgctgggaat ggacagcgtc gaaagttcta tctcttcgaa
      721 cgccatagtg ctgacaacta ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca
      781 cgatcgcggc attaatatgc atgtggtgac tgaagtccag cacattgtga ttcttgcgga
      841 gcatcatctg ctggattaca aaggcgtaga gaaacgattt agccaaattc aaccattacg
      901 ttacccaaga tggctggtag ccttaatggt tggcctttct tgcgcctgtt tctgtaaact
      961 caataacggt ggctgggatg gtgccgtcat caccttcttt gccagtacga ccgcgatgta
     1021 tatccgccag ctgctggcac aacgtcatct tcatccacag atcaactttt gccttaccgc
     1081 tttcgccgcc accaccattt ccggattgct tttgcaactc cccactttca gcaatacccc
     1141 caccattgcg atggccgcca gcgttctgct gctagtgccg ggctttccgt tgattaatgc
     1201 cgtcgccgat atgtttaaag gccacatcaa taccggactg gcacgctggg cgatcgccag
     1261 tctgctgaca ctggctacct gcgtcggcgt agtgatggca ctgacgattt gggggctacg
     1321 cggatgggtg tgatcgaatt tctgttagcg ttggcgcagg atatgatcct cgccgccatt
     1381 cctgcggtcg gctttgcgat ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg
     1441 ctgcttggct cgataggtca tggttcccga atgatcttga tgaccagcgg gttgaatatt
     1501 gagtggtcaa cctttatggc ttctatgctg gtcggtacca ttggtattca atggtcgcgc
     1561 tggtatctgg cgcatccgaa agtgtttacc gtggcggccg ttatccctat gttcccgggc
     1621 atatcggctt ataccgcaat gatttcgtcg gtaaaaatca gccagttagg ttacagcgaa
     1681 ccgttgatga ttaccctgtt aaccaacttt cttacagctt catcgattgt tggtgcgtta
     1741 tccatcggtc tttccattcc tggattatgg ttgtaccgca agcgccctcg cgtataaaat
     1801 tgcctcgctt aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa
     1861 gttatctccc ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt
     1921 gaccccggac gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa
     1981 agctgaaggc ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac
     2041 gcctgcacgc ctggctgaac tgctggcgct ggaagaaaag ctagcgcgtc cgggaagcga
     2101 tgtcgctctg gacgatcaac tctatcagga accgcaagcc gctcccgttg ctgtacccat
     2161 ggggaaattc gccatgtatc cggactggca acccgatgcc gattttatcc gcctggcggc
     2221 gctatggggc gtggcgctaa gagagccggt gaccaccgaa gaactggcct cattcattgc
     2281 ctactggcag gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg
     2341 cagcctgcaa atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt
     2401 cagtgaacct gacagccaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc
     2461 gacctgatgc aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg
     2521 ggtgaagagc tactggcctg gcagaaagaa caaggggcga tccgctccgc cgctctcgaa
     2581 cgtgaaaatc gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcgtccactg
     2641 catcagaact gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta
     2701 agcaaagcgc gccagtatgt cgaagagttt gacggcaaca tcgccagctt catcttttct
     2761 ggtaagccag gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta
     2821 cgcggtaaat ccgtattgat catcaccgtg gccgatatta tgtcggcgat gaaagatacc
     2881 ttcaggaata gcggtaccag cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg
     2941 ctggtgatcg atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac
     3001 cagatcgtcg atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat
     3061 atggaagaga tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac
     3121 agtttatggg tgatcttcaa ctgggatagc taccgtagcc gggtaacggg taaagagtat
     3181 taatccccgt ttctggcggt atactgcgac acaattccac cttgagtaat gatgatgaaa
     3241 actattaaac atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg
     3301 catgctgcgt cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac
     3361 agcacgacac aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcgga
     3421 aaccaagcct taagcgcaga taacatgaac aatgccgcag gcattctgca atactgcgcg
     3481 aagcaaaagc tggcttcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag
     3541 ctgggcctga acagtgaaga gcaaaaagaa gacaccaact atctggacgg tattcagggt
     3601 ttgctgaaaa caaaagatgg tcagcaactc aatctggata acatcggaac gactccgctg
     3661 gcagaaaagg tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct gaacttcatt
     3721 tcctgaaccg cgaaatgtta cccggctgac gcggctctct cctgcccttt tccctgctcc
     3781 tggacggttt tccccctgtt acacgggaag cgaccgcgat caaaaaagtc tgtttataaa
     3841 ctaattccta acaacatcgc atttttttga cactagaatt gcagcaatat ggcgacgcca
     3901 ttacattgtg cttctcaaaa agcttaaacg ttggctaaac ggcccggttt agtgaggatc
     3961 gcccattgtc agaactactc tctttcgccc tttttctcgc ctctgtgctg atttacgcat
     4021 ggaaagcggg acgtaacacc tggtggtttg cagccacgtt aacggtgctg gggctatttg
     4081 tcgttttaaa tatcaccctg tttgccagcg actattttac tggcgatggt attaacgacg
     4141 cggttctcta taccttaacc aacagcctga ccggtgctgg cgtcagcaaa tacatcctgc
     4201 cgggtatcgg cattgtgctg gggctgacgg cggtgttcgg tgcgctgggc tggatcctgc
     4261 gccgtcgtcg tcatcatccg caccattttg gttacagcct gctggcgctc ttactggcgc
     4321 tgggttcggt ggacgccagc ccggcatttc gtcagataac ggaactggtg aaatcccagt
     4381 cacgcgatgg cgacccggac tttgcggctt attataaaga gccgtcgaaa actatccctg
     4441 acccgaaact caacctggtt tatatctacg gcgaaagtct cgagcggacc tattttgata
     4501 acgaagcttt cccggatctc acgcctgaac tgggcgcgtt gaaaaatgaa ggcctggatt
     4561 tcagccacac acagcagctg ccaggaacgg attacacgat tgcgggcatg gtggcttctc
     4621 agtgcggcat accgctgttc gccccctttg aaggcaacgc ctccgcctct gtctccagct
     4681 tcttcccgca gaacatctgt ctgggcgata tcctgaaaaa ctcgggttat cagaactatt
     4741 tcgtgcaggg cgcaaatctg cgttttgccg gtaaagatgt gttcctgaaa tcgcacggct
     4801 tcgaccactt atacggctca gaagagctga aaagcgtggt agccgacccg cactatcgca
     4861 acgactgggg attctacgac gataccgttc tcgatgaagc gtggaaaaag tttgaagagc
     4921 tttcccgctc aggtcagcga ttctcactgt ttaccctgac agtcgatacc catcacccgg
     4981 atggttttat ctcgcgcacc tgtaaccgca aaaaatatga ttttgacggt aagccgaatc
     5041 agtcattcag cgcggtaagt tgcagccagg agaatatcgc gacgtttatc aacaaaatca
     5101 aagcgtcacc gtggtttaaa gatactgtca tcgtcgtctc ttctgaccat ttagcgatga
     5161 acaacacggc gtggaaatac ctcaataagc aggatcgcaa taacctgttt tttgtcattc
     5221 gtggcgacaa gccgcagcaa aagacgctgg cagtgaagcg taacacgatg gataacggcg
     5281 cgacggtgct ggacattctc ggtggcgata actatctcgg acttggtcgt agcagtttat
     5341 ccgggcagtc gatgtcggaa atcttcctca atatcaaaga gaaaacattg gcgtggaagc
     5401 cagatatcat ccgcctgtgg aaattcccta aagagatgaa agagttcacc atcgaccagc
     5461 agaaaaacat gattgccttc tcgggtagcc atttccgttt gccgctgctg ttgcgggttt
     5521 cagacaaacg cgtggaaccg ctgccggaaa gcgaatactc agcaccgctg cgtttccagc
     5581 tggccgattt cgctccacgc gacaatttcg tctgggttga cagttgctac aagatggcac
     5641 aactctgggc tccggaactg gcactttcca ccgactggtg tgtctcgcag gggcagcttg
     5701 gcggtcagca aattgttcag catgttgaca aaacaacatg gaagagcaag acggcattta
     5761 aagatacggt catcgacatg gcgcgttaca aaggcaatgt cgatacgctg aagattgttg
     5821 ataacgatat tcgctacaaa gccgacagtt tcatctttaa cgtcgccggt gcgccagaag
     5881 aggtgaaaca gtttagcggg atttcccgtc cggagtcgtg gggccgctgg tccaacgcgc
     5941 agctgggcga tgaagtaaaa atcgagtaca agcatccgct gccgaagaaa tttgacctgg
     6001 tgattaccgc caaagcatac ggcaataacg ccagccgtcc tattccggta cgcgtaggca
     6061 atgaagaaca aactcttgtg ctgggcaatg aagtgaccac caccacactg catttcgata
     6121 acccaaccga tgccgacaca ctggtaattg tgccgccgga acctgtctca accaacgaag
     6181 ggaatatcct cggacactcg ccgcgtaagc tcgggatcgg catggtggaa attaaagtgg
     6241 tagaacgtga agggtaattc ctgactgatg tgtaggtcgc atagggtgcg acctacacgc
     6301 cttctccggc ttacaacaca ttattaagaa acaaaaaaaa cagcgcccaa cacttccccg
     6361 gacgctgcat ggcaacttta ataatctcgc cttatcagaa agtaattaca cccttaatca
     6421 actcacgatt gttaatcaca tcgcgctcgt aggtttctgc cagcgtggcg aacggatagc
     6481 gatgggttaa catcatgtcg gcagtgattt tcccttccgc catcagtcga ccgactttag
     6541 cgaaatcttc cggcgtggcg ttgcggctgc ccatcatcgt cgtttctttt ttatggaatt
     6601 ccggatcgga gaactgcaac tcacctttaa acagaccgac aaataccacc gtgccgccgt
     6661 gacgaatcag attcacggta ttattcatcg catgttgatt acctgtcgcg tcgatcactt
     6721 tctgcgccag cgaaccacca aactgcgccc gcagctgcgc gtcaaaatct tcggctgacg
     6781 ggtccagcac gggcaactcc agacgcgttg ccacatgttc acggcgcgcc ggactggtat
     6841 ccgccaccac cacctgtgcg ccatcggctt tagcgattgc cgccgcgccc agaccgattg
     6901 gccccgcccc gaccaccagc acctgctcgc cgggagcaat ggctgcgcga cgcaccgcat
     6961 gagcgctaat agcgaaaggt tcaatcaatg cggccgcctg cgggtcaata ccgtctgcgg
     7021 gcaaaatgtt cgccaccggc acgctcaaat actcactaaa accgccatcc tgatgcacgc
     7081 caatgactga aattttttca cagcagttgg tacgcccgct tttacacgcc gggcattgct
     7141 gacaggcaac ataagggatc acagcaactt gctgaccatt tttaagatca gcaatatttt
     7201 tacccagccc aacaatctcc ccacatattt catggcctaa aacccgtgga taactaaaaa
     7261 atggttgatt tccaccctag gcatgaatat cggtaccgca aatcccgaca gacttaattt
     7321 ttattaatgc ttcattgtca cccggaatag gtatctcgcg ttgtttccag actaattctt
     7381 tcggctgctg gcaaattaaa acattcatcg tagacatgat tcggctatcc attttttgtt
     7441 gccttagata tcaatgaaaa tgaaaaatgg cagcggaacg gaaaatctgt tttgtgaaaa
     7501 cacacgcata aaaatgtttt aaatcgggtt ttaatggact cacaaggagg aaaatatgag
     7561 tcgttcacaa aatttacgcc acaatgtgat taaccaggtc attgatgata tggcgcgggg
     7621 tcatatccct tccccacttc catcgcaaag tgcgctggcg gaaatgtaca acatcagccg
     7681 cactacagtg cgtcatattc tcagccaatt acgcgaatgc ggcgtcttaa cgcaggttgg
     7741 aaacgactat gttattgcgc gtaaacctga ccatgatgac ggtttcgcct gtactaccgc
     7801 ttcaatgagt gaacaaaaca aagtgtttga gcaggctttt ttcacgatga taaaccagcg
     7861 tcagttacgc cctagggaaa ctttttctga actgcaactg gctcgggccg cgggtgtcag
     7921 cccggtggtt gtaagagaat atcttttaaa attcggccgt tataatctta ttcaaagcga
     7981 aaagcgcggc cagtggagca tgaagcaatt cgatcaatcc tacgccgagc agctgtttga
     8041 gctacgcgaa atgctcgaaa cacattcact tcagcacttt ctcaacctgc ctgatcatga
     8101 tccccgctgg ctgcaggcga aaaccatgct agaacgtcat cggctactgc gtgacaacat
     8161 cggcaatagc tttcgcatgt tctcgcaact tgacagagat tttcactcct tactgctttc
     8221 agctgccgac aatatctttt ttgatcaatc gcttgagatc atctcagtca tctttcattt
     8281 tcactatcaa tgggacgaaa gcgatcttaa acagcgcaat atcattgccg tcgatgaaca
     8341 tatgaccatt ctcagcgctc tgatttgccg cagtgacctt gatgccaccc ttgcgctccg
     8401 taaccatttg aattcagcta aacaatcaat gattcgatcc attaatgaga acacgcgtta
     8461 tgctcattaa gtacggatta aaaacagcaa actattagcg ggtgcacggt tttatcaaaa
     8521 gataaatacc gggaaattcc cgcttaccta tgctcacaat cagtcgacta aaaatgtttt
     8581 tgtgagcggt agtaaagtcc taaaacttta acctgccgtt aatgtcaacc ttgcgtaatg
     8641 cacaattacg taaacaatta aaattataaa tacaggagtc cggcgtggaa aaagaaaata
     8701 tcaccctcga tccgcgttct tcatttactc catcttcgtc ggcagatatt cccgtgccac
     8761 cagatggatt agttcaacgc agtacccgaa ttaaacgcat tcaaaccacc gccatgttgt
     8821 tattattttt tgcggcggta atcaattatc tcgaccgcag ttcgctgtcg gtagcaaatt
     8881 taacgattcg tgaagaattg ggattaagtg ccaccgaaat cggcgctttg ctctccgtgt
     8941 tttcactcgc ttacggcatt gcgcaacttc cttgcggccc actattggat cgtaaaggcc
     9001 cacgcctgat gctgggactg gggatgttct tctggtcact gttccaggcc atgtctggca
     9061 tggtgcacag ctttacgcag ttcgtgttgg tgcgtatcgg tatggggatt ggtgaagcgc
     9121 cgatgaaccc atgcggtgta aaagtcatta acgactggtt caacatcaaa gagcgcggac
     9181 gcccgatggg cttcttcaac gcagcttcta ccattggcgt tgctgtaagc ccaccgattc
     9241 tggcggcaat gatgctggtg atgggctggc gcgggatgtt tattaccatt ggtgtactgg
     9301 ggatttttct cgccatcggc tggtatatgc tctatcgcaa ccgcgagcac gtagaactga
     9361 ctgccgttga acaagcttat ctcaatgcag gtagcgtcaa tgcccgccga gatccgctca
     9421 attttgccga atggcgcagc ctgttccgta accgcacaat gtggggaatg atgctcggat
     9481 tcagtggcat caactacact gcgtggctgt atctggcctg gcttcctggt tacctgcaaa
     9541 cagcctataa cctggattta aaaagcacag ggttgatggc ggctatccct ttcctgtttg
     9601 gggctgccgg gatgctggtc aacggttacg ttactgactg gctggtcaaa gggggaatgg
     9661 ctccgattaa aagccgtaag atctgcatta ttaccgggat gttctgttct gccgccttta
     9721 cgctgatagt accacaagcg acaacatcca tgatggcggt tctgctgatt ggcatggcac
     9781 tgttctgtat tcactttgcc ggaacatcct gctggggctt gatccacgtc gcagttgctt
     9841 ctcgcatgac tgcgtcggtg ggcagtatcc agaactttgc cagcttcatc tgcgcctctt
     9901 tcgcgccgat cattactggt tttattgttg atacaaccca ctcattccgt ctggcactaa
     9961 tcatttgcgg ttgcgtcacc gcagcgggag cactggcgta catcttcctg gttcgccagc
    10021 cgatcaacga cccgcgtaaa gattaaactc cctatgcccg ataacatttt gcttatcggg
    10081 catttttatg gcgattaaaa cgtttcccag ttctccccgc tatctgccac tgccatttta
    10141 cgcggcgtag ctggcgtcac ggtttttacc acagccgatg tttcacgctg ctgttgctgc
    10201 tgttgctgaa tccggaacac tgccacagct tcggtcaggc gactggcctg ctcttccagc
    10261 gcggcggcgg cagcggcaga ttcttccacc agcgcggcgt tctgttgagt tacccggtcc
    10321 atctcagcaa ccgctaagcc aacctgatcg ataccacggc tctgctcatc agaagcagaa
    10381 gatatttcgc ccataatgtc cgtcacgcgg gtcacagcac tgacaatctc cgccattgtt
    10441 tccccggcgc tttcgaccag cgtagagcca acatccactt tccccaccga gtcttcaatc
    10501 aggcttttaa tttcacgaac cgcctgggcg ctacgctggg ccagattacg cacttctccc
    10561 gcaaccaccg caaaaccacg tccttgctca cccgcacgcg ctgcttcaac cgccgcgttc
    10621 aaagccagaa tattggtctg gaaggtaatg ccgtcaatta cgctgataat atcggcgatt
    10681 ttctgcgaac tggtggagat atcgcgcata gtctgcacca cgttatctac cactttgccg
    10741 ccgcgttgcg ccgtttcaga agcacttaat gccagatggc tggcctggcg cgcattctcg
    10801 gcgttctgtt tcaccgttgc ggtcagttgc tccatgctgg ctgccgtctc ttccagcgaa
    10861 gcggcctgtt gctcggtgcg cgaagagaga tcattattgc cggtagcgat ttcgctggca
    10921 ccgctataga tggcattggc cccgttgcgc acatcaccga cggtacgcat cagctctccc
    10981 tgcatatggc gcaaactctc tgccagttgc cccatctcat tagagccatc cacctcaatc
    11041 ggtttcacca gatcgccgcc tgcaatatga cgaatgctgt caatcaggcg attcattggc
    11101 gctaccagcg aggctttaat accgaaccag acggcgaaga tgaccgccag tacgacgatc
    11161 atcacgccca ccagaatcca catcgcctgg ctgtaggagg cattgttatc gctgacggcg
    11221 atatcataga gccgatcgtt ttgctccatg taagccacat actgcttctc gaaaccgtcc
    11281 tgatatccct gggtcggctg atcaaagaac tcgttgattt tgcctgcacc taacagttgg
    11341 atcagctccg ccagcgcatt gtgataaata tcgtaattac gtttgatctc tgccgctgcg
    11401 gcggtgctct gacgcgggtc acgcggcaac gcttcgtaat ccgcccagtt tttttccgcc
    11461 tgtttcagcg aaatactggc actctgcatc agctcagcaa cggttgaacc gctaccaata
    11521 ttattctgat ccatcatgta gcggataccc gcgcggttga gggtgttacg cgtctgcaac
    11581 aacgcgaccc agctgccatt cagcgtgggt tgctgctggc gaatggtttg taaaacagtg
    11641 aaattttctt tgtcattctt taaggcatta aaaaacagac cgcctgatgt cagttgtaaa
    11701 aggccaaaaa cggccaaaac cagcagtaag ctggtcacga tcttgatacg ttttaacatg
    11761 gtttctcttt cctgtggacc gatgcaagat tttcggcctg gaaagcaaaa actttatgaa
    11821 aaggtgtaac ccgccatcca tggaaaaacc aggcgctggt tatcagatac taaaaagtta
    11881 ttcattatcg cccgggatgt cttcttgtcg ggaattgccc agagcaataa attcctctaa
    11941 cagatgggtt aactgttgca ttttctcggc agtaaactga gcttcaatct gccgataggc
    12001 ttcttcaatc tgtgtctgag cgcggttata cagcgcctgc ccctctttgg tcagcgagat
    12061 atagagcttc cgctgatcat tgatcggctt taatcgcaac actaaaccgt cgcgttccat
    12121 ccgcgtgagg atcccggtca ggcttgggcg caaaatgcag gcgcgatacg ccagatcgtg
    12181 aaaatccatt gatgggcttt ccgccagaat acgcacgatg cgccactgtt gctcagtcaa
    12241 attatgccgt ttaacgattg ggcgaaaata actcatcgcc gcttctcgcg cctggagcaa
    12301 cgcaatggtt agtgagtcgt gcattatctt tcccctgaaa aattcaaaat cattaatata
    12361 gaaacaatta atcatcacgt taccgggact gagctgcccc cgcaattctt cattttatca
    12421 aaattgatga tattttatta acttactgat atttaatatt ttattatctt taaatgtaaa
    12481 tagtttgtta attagatcac atttatatca cttacttttg ccaaactacg caatcgggaa
    12541 taaaagtaat cattaacata ttaatgatta agatgatcga atccgaggag tggtaatgaa
    12601 aggcactatc ttcgccgtag cgttgaacca tcgcagccaa cttgatgcct ggcaggaagc
    12661 gttccagcaa tctccctaca aagccccgcc taaaactgcg gtctggttta ttaaaccgcg
    12721 caatacggtg attggttgcg gtgaaccgat tccctttcca cagggtgaaa aagtactgag
    12781 cggtgcgact gttgcgctga ttgtggggaa aacggcgacg aaagtacgtg aagaagatgc
    12841 ggcagagtac atcgccggat atgcgctgga taacgacgtc agcctgccgg aagagagctt
    12901 ttaccgtccg gcaatcaaag caaaatgtcg tgatggattc tgccccattg gcgaaaccgt
    12961 ggctctcagc aatgtcgata atctgactat ctataccgag atcaacgggc gtcctgccga
    13021 tcactggaat accgccgatt tacaacgtaa cgccgcacag ctgctgagcg ccctgagcga
    13081 atttgccacg ctgaatccag gcgatgctat tctgctcggc acgccacagg cgcgcgtgga
    13141 aatacagcca agcgatcgcg tgcgtgttct cgcagaaggt ttcccgccgc tggaaaatcc
    13201 ggtagtggac gaacgtgaag tgaccacgcg caagagcttc ccaacgctgc cacacccgca
    13261 cggtacgctg tttgccctcg gcctgaacta cgccgaccac gccagcgaac tggaatttaa
    13321 gccaccggaa gagccgctgg tgttcctgaa agcgccgaat accctcactg gcgacaacca
    13381 gacctccgtg cgcccgaaca atattgaata catgcactac gaagcggagc tggtggtagt
    13441 tattggcaag caggcgcgta acgtcagcga agccgatgcc atggattatg tcgcgggcta
    13501 caccgtgtgt aacgactacg ccattcgcga ctatctggaa aactactacc gccctaacct
    13561 gcgggtcaaa agccgcgacg gactgacgcc gatgctttca accatcgtgc cgaaagaggc
    13621 gatcccggac ccgcataatc tgacccttcg caccttcgtc aacggcgagt tacgccagca
    13681 aggcaccacc gccgatctga tcttcagcgt gcccttcctg atcgcctact taagcgaatt
    13741 tatgaccctg aatccgggcg acatgatcgc caccggcaca ccaaaaggct tatctgacgt
    13801 ggtgcctggc gatgaagtag tggtggaagt agaaggcgtg ggccgcctgg tgaaccgaat
    13861 tgtgagtgag gaaacagcga aatgaaaaaa gtaaatcatt ggatcaacgg caaaaatgtt
    13921 gcaggtaacg actacttcca gactaccaat ccggcaacgg gtgaagtgct ggcggatgtg
    13981 gcctctggcg gtgaagcgga gatcaatcag gcggtagcgg cagcgaaaga ggcgttcccg
    14041 aaatgggctg atctgccgat gaaagagcgt gcgcgcctga tgcgccgtct gggcgatctg
    14101 atcgaccaga acgtgcctga gatcgccgcg atggaaaccg cggacacggg cctgccgatc
    14161 catcagacca aaaatgtgtt gatcccacgc gcttctcaca actttgaatt tttcgcggaa
    14221 gtctgccagc agatgaacgg caagacctat ccggttgacg acaagatgct caactacacg
    14281 ctggtgcaac cggtaggcgt ttgtgcgctg gcgtcgccgt ggaacgtgcc gtttatgacc
    14341 gccacctgga aggtcgcgcc gtgtctggcg ctgggcaata ccgcggtgct gaagatgtcg
    14401 gaactctccc cgctgaccgc cgaccgcctg ggtgagctgg cgctggaagc tggtattccg
    14461 gcgggcgtgc taaacgtggt acagggctac ggcgcaaccg caggcgatgc gctggtccgt
    14521 catcatgacg tgcgtgccgt gtcgttcacc ggcggtaccg ccaccgggcg caacatcatg
    14581 aaaaacgccg ggctgaaaaa atactccatg gagctgggcg gtaaatcacc ggtgctgatt
    14641 tttgaagatg ccgatattga gcgtgcgctg gacgccgccc tgttcaccat cttctcaatc
    14701 aacggcgaac gctgcaccgc cggttcgcgc atctttattc agcagagcat ctatccagaa
    14761 ttcgttaaac gctttgccga acgcgccaac cgtctgcgcg tgggcgatcc gaacgatccg
    14821 aatacccagg ttagggcgct tatcagtcag caacactggg aaaaagtctc cggctatatc
    14881 cgtctcggca ttgaagaagg cgcaaccctg ttggcgggcg gcccggataa accgtccgac
    14941 ctgcctgcgc acctgaaagg cggcaacttc ctgcgcccaa cggtgctggc ggacgtagat
    15001 aaccgtatgc gcgttgccca ggaagagatt ttcgggccgg tcgcctgcct gctgccgttt
    15061 aaagacgaag ccgaaggctt acgcctggca aacgacgtgg agtacggcct cgcgtcgtac
    15121 atctggacac aggatgtcag caaagtgtta cgcctggcgc gtggcattga agcaggcatg
    15181 gtgttcgtca acacccagaa cgtgcgcgac ctgcgccagc catttggcgg cgtaaaagcc
    15241 tccggcaccg ggcgtgaagg cggtgagtac agcttcgaag tgttcgcgga aatgaagaac
    15301 gtctgcattt ccatgggcga ccatccaatt ccgaaatggg gagtctgata tgggtaagtt
    15361 agcgttagcc gccaagatca cgcacgtacc gtcgatgtat ctctctgaac taccaggaaa
    15421 aaaccacggt tgtcgccagg gtgcgatcga cgggcataaa gagatcagca agcgttgccg
    15481 ggaaatgggc gtcgatacca ttatcgtttt cgatactcac tggctggtca acagtgctta
    15541 tcacatcaac tgtgcagacc attttgaagg cgtctacacc agcaacgagc tgccgcattt
    15601 tattcgcgac atgacctaca actacgaggg caacccggag ttggggcagc taattgccga
    15661 ggaagcctta aagctcggcg tgcgggcaaa agcgcacaat attcccagcc tgaaactgga
    15721 atacggcacg ctggtaccga tgcgctacat gaatgaagac aagcacttca aagtggtctc
    15781 catttcggct ttctgcacgg ttcacgattt tgccgacagc cgcaagctgg gcgaagcgat
    15841 tctgaaagcg atcgaacagt acgacggcac cgtggcggtc cttgccagcg gttcgttatc
    15901 gcaccgcttt attgacgatc agcgtgcgga agaagggatg aacagctaca cccgcgagtt
    15961 cgaccgccag atggacgagc gtgtggtgaa gctgtggcgc gaaggccagt tcaaagagtt
    16021 ttgcaatatg ctgccggagt acgccgacta ctgctacggc gaaggcaata tgcacgacac
    16081 ggtgatgctg ctggggatgc tcggctggga taaatacgac ggcaaggtgg agtttattac
    16141 cgagctgttc ccaagctctg gcaccggtca ggttaacgct gttttcccgc tgcccgcgta
    16201 aggacgtttt atgccgcact ttatcgtgga atgtactgaa aatattcgtg aagaagcccg
    16261 cttacctgag ctgtttgcca gtgttaacac tgcactggcg gcaacgggga tttttccgct
    16321 tgggggaata cgtagccggg cgcactggat cgatacctgg caaatggctg atggcaagca
    16381 cgattacgcc tttgtgcata tgacgctaaa aatcggcagc ggacgcagtc tggaaagccg
    16441 tcaggaagtg ggcgaaatgc tgtttgatct gattaaaacg cacttcgcct cactgatgga
    16501 gagccgttac ctagcgcttt catttgagat tgcagagctg cacccgacgt tgaatttcaa
    16561 acagaacaac gtacacgcgt tgtttaaata aatttgcacc agcttccagg ccggatgata
    16621 aggcgctgcc gcatccggca atgccacagg atatcgctat gttcgataaa cacacccaca
    16681 ccctgatcgc ccagcgtctg gatcaggcag aaaaacagcg cgaacagatc cgcgcgatct
    16741 cgctggatta cccggagatc accatcgaag acgcctacgc cgtgcagcgc gaatgggttc
    16801 gcctgaaaat cgccgaaagt cgcacgctga aaggccacaa aatcggtctg acctcgaaag
    16861 cgatgcaggc aagctcgcag atcagcgaac cggattacgg tgcactgctg gacgacatgt
    16921 tcttccacga tggcagcgat atcccgaccg atcgctttat cgtgccgcgc attgaagtgg
    16981 agctggcttt tgtgctggca aaaccgctgc gtgggccaaa ctgcacgctg ttcgacgttt
    17041 acaacgccac ggactatgtg atcccggcgc tggagctgat cgacgctcgc tgccacaaca
    17101 tcgatccgga aacccagcgc ccgcgcaaag tattcgacac catttccgat aacgccgcca
    17161 acgccggagt gatcctcgga ggtcgtccga tcaaaccaga cgaactggat ctgcgttgga
    17221 tctccgccct gatgtatcgc aatggcgtga ttgaagaaac cggcgtcgcc gctggtgtgc
    17281 tgaatcatcc ggcaaacggc gtggcctggc tggcgaacaa actcgcaccg tatgacgtac
    17341 aactggaaac cgggcaaatc attctcggtg gttcgttcac cctcccggtt ccggcgcgta
    17401 agggcgacac cttccacgtc gattacggca acatgggctc cattagctgc cgctttgttt
    17461 aaggagagaa cgatggaaaa cagttttaaa gcggcgctga aagcaggccg tccgcagatt
    17521 ggattatggc tggggctgag cagcagctac agcgcggagt tactggccgg accaggattc
    17581 gactggttat tgatcgacgg tgagcacgcg ccgaataacg tgcaaaccgt gctcacccaa
    17641 ctacaggcga ttgcgcccta ccccagccag ccagtggtac gcccatcgtg gaacgatccg
    17701 gtgcaaatca aacaactgct ggacgtaggc acacaaacct tactggtgcc gatggtacaa
    17761 aacgccgacg aagcccgtaa agcggtacgc gccacccgtt atccccccgc cggtattcgc
    17821 ggtgtgggca gtgcgctggc tcgcgcctcg cgctggaatc gcattcctga ttacctgcaa
    17881 aaagccaacg atcaaatgtg cgtgctggtg cagatcgaaa cgcgtgaggc aatgaagaac
    17941 ttaccgcaga ttctggacgt ggaaggggtc gacggcgtgt ttatcggccc ggcggatctg
    18001 agcgccgata tgggttatgc cggtaatccg cagcacccgg aagtacaggc cgccattgag
    18061 caggcgatcg tgcagatccg cgaagcgggc aaagcgccgg ggatcctgat cgccaatgag
    18121 caactggcaa aacgctatct ggaactgggc gcgctgtttg tcgccgtcgg cgttgacacc
    18181 accctgctcg cccgcgccgc cgaagcgctg gcagcacggt ttggtaatag ttcttctatt
    18241 tcgatggcga agcaaaacaa caactctgtc tactaaacgt acgatgtgga gcgcatcatg
    18301 acgacctcta ccctgcaata taaagataat aaaactgttg aaattgaaca tcgtgtcatt
    18361 aataaattat ttcgtcgcct aatcattttt ctttttattc tgtttgtttt ttcgtttctt
    18421 gatcgcatca atataggatt tgccggactg acaatgggca aggatcttgg cctgacgtcc
    18481 acaatgtttg gcctcgccgc aacactcttt tatgtcacgt acgtcatgtg cgggatcccc
    18541 agtaatatga tgttagcgaa aattggcgct cgtcgatgga tcgctgggat catggtggtc
    18601 tggggaattg cttccacctg cacaatgttt gcaacaagcc ccgaaacact ctatctactg
    18661 cgtatgctgg ttggtattgc tgaggcgggt tttttacctg gtattttggt ttatctgacc
    18721 tggtggttcc cggcatatca tcgggccaga gccaatgcct tatttatgat agcgatgcca
    18781 gtcactatga tgctgggatt gatcctttca ggctacattc tggccttgga tggactgtgg
    18841 aatctaaaag gctggcaatg gctctttttg ctggaaggtc tgccatcggt cattcttggt
    18901 gtcgtcacct ggttttatct caacgacaca ccagaccagg cgacctggct tgatgaaaac
    18961 gaaaaacagg ctttaaaaac gatgattctc cgtgaacaag agagtgcatt tgcccatgca
    19021 tctgttccac gctcaacctt acgtgaagtc ctgaccccag ccatcatgct atatacactt
    19081 gcttattttt gcccgactaa tactctgagt gcgattaata tctggacgcc gcaaatccta
    19141 cagagtttca atactggcag tagcaacatc atgattggtt tgctggcggc catcccccaa
    19201 ttctgcacaa tttttggcat gatctggtgg agtcgtcgtt ccgaccgtct taaagaacgc
    19261 aaaaagcata cgattcttcc ctacctgttt gccgctgccg gatggttact cgcttctgcg
    19321 acagatcaca gcctcattca actgagcagc atcattatgg cctcaatggg atcatttacc
    19381 gcgatgacta tcttttggac aacaccggat caagttataa gcttgcaatc gcgtgccgtt
    19441 gcgatggctg tcatcaatgc tgtcggcaat gtaggttcag cagtcagtcc attacttatc
    19501 ggtattttga aagatgcaac cggcagtttt agctcaggtt tgtggtttgt agcaggattg
    19561 ctggtactgg gcgcgttagt catgacccga atccctatgg ggagtcggga acactctctt
    19621 tcatctgaac ttatcgcacc aaaaccgcaa taaggagtca tgatgtccag tgaagctatc
    19681 tttgaacatg gttatgaaaa tattgatttg gtccgggaat atgatgcccg ctacgctggt
    19741 gaagatgtgc attatgaaac atttgcccgg ttagcgaatt tctttggacg cgatatgcgc
    19801 cctcactggc acgatagata ttttcaactc cattatctgg tcactggacg cattacttta
    19861 caactggatg agcacttcta ttctgtgcgc gcaccgcttt ttgtactaac accaccatcg
    19921 gtgccacata ctttctttac tgatcctgat agcgatgggc atgttctgac cgtacgtcag
    19981 gagttaattt ggcccctggt agaaaagctc tggcctggta gtggagatgc cgcaatgcta
    20041 caaggtatct gtctgtcact ggagggaatg gaacatacac tggcggcatt aaatcattat
    20101 tggccgataa tatccctgga gtttcaacaa aataagcaag gacgggaaat tctattgcaa
    20161 tcactggcgc aaagtatttt tacaatccta ctgcgtgaag caccaccaaa tgatatttca
    20221 acatgcagcg tgcgcggcga gatgtgttta ttccagaagt ttaatcggct gattgacgaa
    20281 aattatcgcc aacatttaat ggtccctgaa tatgcaacaa tgctcggtat gagtgaatca
    20341 cggcttaccg agctgtgccg acgttttgcc aatcaatcgc cgaagagatt gattttcgaa
    20401 cgagtgagcc gggaagccag acggcttctg ctctatagcg agcaaagcat caaccagata
    20461 gcccttgatc ttggctacaa agatcccgcc tatttcgccc gttttttcaa ccgaatgatg
    20521 ggttgctcac caacacaatt ccgtggtcgc tgaccattta tcaacctgag agactcgaca
    20581 tgaaaatatc tttacgctca ctatttgtgg cgtgcctgat tgcgccagtg gcattcacct
    20641 acgcctccga accgcaataa atgaaaattg tactacttgg aacgaaaggt ggcccttctt
    20701 tattaaaagc tgaccgttta ccgatggcaa cggcacttat tattggcaat aaaacctggt
    20761 taattgatgc tggttacggg gccagcttac aactggtaaa aaataatatt ccactgcgcc
    20821 agattgataa tattttactc acccatttac acagtgatca tattctggac tatccatcag
    20881 tcttaatgaa tgcatgggct agcggtctgc aggacaaaac tattcatgta ttcggtccgc
    20941 caggcacccg agccatgacc gctgccagtt ggcaggtctt cgatcgggac atcacactgc
    21001 gtatggaaga agaaggaaaa cccgatcctc gcaaactcgt tacagctcaa gatgttggtg
    21061 aaggcgtagt ttataaagat gataccgtga ccgtgactgc attaaaggtc cctcattctc
    21121 ctttccctga cggtgaggct tttgcttatc gcttcgacac acaaggcaag cgaattgtct
    21181 tctcaggaga tacctcgtac tttcccccac tggcaacttt ctcagaaggc gctgatatac
    21241 ttgtgcatga ggccgtacat gagccctccg tcgctaagct ggcagacagt attggcaatg
    21301 gtaaaacact ggcaaaagcc attgcttctc accatacgac tattgaggat gtaggcaaaa
    21361 ttgccaatga agcaaaagtg aaaaaactca tccttagcca cctggtacca atcaccgttc
    21421 cggatgaaac atggaagcaa gaagcacaga aaactattca ggtcctatcg tcgttggtca
    21481 tgaaaatatg acgattacca ttccattcgc cccataaaaa attccgtccc cgttagccct
    21541 tgttagcggg gaatattatg atttgcctta ctgtaagttg atcacgatcg cagtttacta
    21601 tttaactcca ccttgctgtc gaaaagtacc aacgttcgcc tgaaacgtct cttcccgaca
    21661 accgcgttca acgcttcaat taaataacaa aaacaacaca caaaattaac aatcgtattc
    21721 cgattaatac tgtagaggtc gacatgaaac cagaagattt ccgcgccagt acccaacgtc
    21781 ctttcaccgg ggaagagtat ctgaaaagcc tgcaggatgg tcgcgagatc tatatctatg
    21841 gcgagcgagt gaaagacgtc accactcatc cggcatttcg taatgcggca gcgtctgttg
    21901 cccaactgta cgacgcgctg cacaaaccgg agatgcagga ctctctgtgt tggaacaccg
    21961 acaccggcag cggcggctat acccataaat tcttccgcgt ggcgaaaagt gccgacgacc
    22021 tgcgccagca acgcgacgcc atcgctgaat ggtcacgcct gagctatggc tggatgggcc
    22081 gtaccccaga ctacaaagcc gctttcggtt gcgcactggg cgcgaatccg ggcttttacg
    22141 gtcagttcga gcagaacgcc cgtaactggt atacccgtat tcaggaaact ggcctctact
    22201 ttaaccacgc gattgttaac ccaccgatcg tcatttgccg accgataaag tgaaagacgt
    22261 ttacatcaag ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt
    22321 tgccaccaac tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat
    22381 gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtgaa
    22441 attaatctcc cgcgcctctt atgagatggt cgcgggtgct accggctcgc catacgacta
    22501 cccgctctcc agccgcttcg atgagaacga tgcgattctg gtgatggata acgtactgat
    22561 cccatgggaa aacgtgctga tctaccgcga ttttgatcgc tgccgtcgct ggacgatgga
    22621 aggcggtttc gcccgtatgt atccgctgca agcctgtgtg cgcctggcag tgaaactcga
    22681 cttcattacg gcactgctga aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg
    22741 tgtgcaggcc gatctcggtg aagtggtggc gtggcgcaac accttctggg cattgagtga
    22801 ctcgatgtgt tcagaagcga cgccgtgggt caacggggct tatttaccgg atcatgccgc
    22861 actgcaaacc tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa acattataga
    22921 acgcaacgtt accagtggcc tgatctacct cccttccagt gcccgtgacc tgaacaatcc
    22981 gcagatcgac cagtatctgg cgaagtatgt gcgcggttcg aacggtatgg atcacgtcca
    23041 gcgcatcaag atcctcaaac tgatgtggga cgccattggc agcgaatttg gtggtcgtca
    23101 cgagctgtat gaaatcaact actccggtag ccaggatgag attcgcctgc aatgtctgcg
    23161 ccaggcacaa agctccggca atatggacaa gatgatggcg atggttgatc gctgcctgtc
    23221 ggaatacgac cagaacggct ggactgtgcc gcacctgcac aacaacgacg atatcaacat
    23281 gctggataag ctgctgaaat aacgcagcag gaggttaaga tgcaattaga tgaacaacgc
    23341 ctgcgctttc atgacgcgat ggccagcctg tcggcagcgg taaatattat caccaccgag
    23401 ggcgacgccg gacaatgcgg gattacggca acggctgtct gctcggtcac ggatacacca
    23461 ccgtcgctga tggtgtgcat taacgccaac agtgcgatga acccggtttt tcagggcaac
    23521 ggcaagttgt gcgtcaacgt cctcaaccat gagcaggaac tgatggcgcg ccacttcgcg
    23581 ggcatgacag gcatggcgat ggaagagcgt tttagcctct catgctggca aaaaggtccg
    23641 ctggcgcagc cggtgctaaa aggttcgctg gccagtcttg aaggagagat ccgcgatgtg
    23701 caggcgattg gcacacatct ggtgtatctg gtggagatta aaaacatcat cctcagtgca
    23761 gaaggtcacg gacttattta ctttaaacgc cgtttccatc cggtgatgct ggaaatggaa
    23821 gctgcgattt aagctccagc cccttcacgc attcgtcgaa ggggcatttt tattgtgatc
    23881 tcaatcacat atctacccga tgaataattc ttgcccaacc gctcaccgcc attcctgacc
    23941 gcttacatcc ctaaaataac cactcagtta tttaccttac tttacgcgca cgtatctctg
    24001 gcaacatcac tgcaggatag cggtcaattt acctcctcaa acgcaacgca aacctagaac
    24061 ggcttcggcc tatgattaac aaattactct atggattttg gtttgcagga aggcagcaag
    24121 tgagtgaatc cccgggagct tacaaaagta agtgtcagag gtgaacgaac gcagctgccg
    24181 cacctgtaag ccaaaagacg acgagtaaaa tgccaggttt tactatggat acgaaaaaac
    24241 tattcaagca cataccctgg gtgattctcg gaatcatcgg tgcattctgc ctcgcggtag
    24301 ttgcattacg tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc tctgtgtcgg
    24361 tgtatctggt ggcgtatcgc tactacagtc tgtacatcgc ccagaaggtg atgaaactcg
    24421 accccacgcg cgcgacgcct gcggttatta acaacgacgg tctgaactac gttccgacca
    24481 accgttacgt gttgtttggt caccacttcg ccgctatcgc cggtgctggt ccgctggtgg
    24541 gaccggttct cgccgcgcag atgggctacc tgcctggcac gctgtggctg ctggcggggg
    24601 tagtgctggc cggtgcggta caggacttta tggtgctgtt tatctcctct cgccgtaacg
    24661 gcgcatctct cggtgagatg atcaaagaag agatggggcc agtaccgggg actatcgcgc
    24721 tgtttggctg tttcttaatc atgatcatca tcctcgccgt tctggcgctg attgtggtta
    24781 aagccctggc cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta ccgattgcgc
    24841 tgtttatggg tatctacatg cgctttatcc gtccggggcg tgtgggtgaa gtctctgtta
    24901 ttggtatcgt gctgctggtt gcctctatct acttcggtgg cgtgattgct cacgatccgt
    24961 actggggtcc ggcactgacc tttaaagaca ccaccattac cttcgcgctg attggctatg
    25021 cgtttgtttc cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac tatctggcaa
    25081 ccttcctgaa aatcggcgtt atcgtcggcc tggcgctggg tatcgtggtg ctgaacccgg
    25141 aactgaaaat gcctgcgatg acccagtaca ttgacggtac tggcccgctg tggaaaggcg
    25201 ctctgttccc gttcctgttc atcaccatcg cctgtggtgc ggtatctggc ttccacgcgc
    25261 tgatctcttc cggtacgacg ccgaaactgc tggctaacga aaccgacgcg cgtttcattg
    25321 gctacggcgc aatgctgatg gagtccttcg tggcgattat ggcgctggtt gcagcgtcca
    25381 tcatcgaacc gggtctttac ttcgcgatga acaccccgcc tgctggcctt ggcatcacca
    25441 tgcctaacct gcatgaaatg ggtggcgaga acgcgccgat catcatggcg cagctgaaag
    25501 acgttaccgc acacgcggca gcgaccgtca gctcctgggg cttcgtgatt tcgccagagc
    25561 agatcctgca aaccgcgaaa gacattggtg agccttctgt cctgaaccgt gcaggtggtg
    25621 cgccgacgct ggcggtaggt atcgctcacg tgttccacaa agtgctgccg atggctgaca
    25681 tgggcttctg gtatcacttc ggtattctgt tcgaagccct gttcatcctg accgcgctgg
    25741 atgcgggtac ccgttctggc cgctttatgc tgcaagacct gctgggtaac ttcatcccgt
    25801 tcctgaaaaa aaccgactct ctggttgccg gtatcatcgg tactgcgggc tgtgtgggtc
    25861 tgtggggcta cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt aagagcctgt
    25921 ggccgctgtt cggtatctcc aaccagatgc tggcagccgt agcgctggta ctgggcaccg
    25981 ttgtgctgat taagatgaag cgcacccaat acatctgggt aactgttgtt ccggctgtat
    26041 ggctgcttat ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc aacccgcaga
    26101 tggaaggctt cttctacatg gcaagccagt acaaagagaa gattgctaac ggtactgacc
    26161 tgacggcgca gcagattgcc aacatgaacc acatcgttgt gaacaactac accaacgcag
    26221 gcctgagtat tctgttcctg attgtggtgt acagcatcat cttctacggt ttcaaaacct
    26281 ggcttgcggt gcgtaacagc gacaaacgta ctgacaaaga aacaccgtac gttccaatcc
    26341 cggaaggcgg cgtgaagatc tcttcgcacc actaaccgtg tttagccccg cttcggcggg
    26401 gctttgttct tatcagagtg aactatgttt ggtaacttag gacaggcaaa aaaatatctc
    26461 ggccaggcgg cgaagatgtt gattggtatt ccagactacg acaactacgt cgagcatatg
    26521 aagaccaacc atcccgacaa gccgtacatg agctatgaag aattcttccg cgaacgccag
    26581 aacgcgcgct acggcggcga tggtaaaggc ggtatgcgct gttgttaatg gagagagcat
    26641 gaacccgatt gcagttaccc tactcaccgg ttttttaggc gcgggcaaaa ctaccctgct
    26701 gcgccatatt cttaacgaac aacacggcta caagattgcc gtgattgaaa acgaattcgg
    26761 cgaagtctcc gttgatgacc aactgattgg cgaccgcgcc acgcagatca aaacgctgac
    26821 caacggctgc atctgctgtt cgcgctccaa cgagctggag gacgcactac tcgacctgct
    26881 ggataatctc gacaagggca atattcagtt cgatcgtctg gtgattgaat gcaccggcat
    26941 ggccgatccc ggcccgatta ttcagacctt tttctcccat gagattttat gccagctcta
    27001 cctgctggac ggcgtgattg cgctggtgga tgcggtacat gccgatgagc agatgaacca
    27061 gttcaccatc gcccagtccc aggtgggcta cgccgaccgt attctgctga ctaaaaccga
    27121 cgtcgcaggc gaagcagaga aactgcgtga acgcctggcg cgcatcaacg cccgcgcacc
    27181 ggtctacacc gtcacccacg gcgacatcga cctgggcctg ctgttcaaca ccaacggttt
    27241 tatgctggaa gagaacgtcg tcagcaccaa accgcgtttc cactttatcg cggataaaca
    27301 aaacgatatt tcgtcaattg tggtggaact ggattacccg gtagatatca gcgaagtttc
    27361 ccgcgtgatg gaaaacctgc tgctggaatc ggcggataaa ctgctgcgtt acaaagggat
    27421 gctgtggatt gacggcgaac ctaaccgcct gctgttccag ggcgtccagc gcctctacag
    27481 cgccgactgg gacaggccgt ggggcgatga aaaaccgcat agcacaatgg tgtttatcgg
    27541 gattcagttg ccggaggaag agattcgggc ggcgtttgcg ggattgagga agtaagttcg
    27601 ggaaagatca actttttgag agttccaccg caacattatt acgagtggaa ctctttagat
    27661 atgtcagacc aaccaaagct tcttcaattg cgttttcatt ttttcatgtc tcattttcag
    27721 ttcagccaat ccccattctg aagaattaca aacatctttt gttaaataaa aagagctttt
    27781 actattaagt ttctcataaa tagatttctt tttgataaaa tcatagtgtt gtgtttcaga
    27841 atttttggaa ccacttatga gtgttaaatt acctatttta tgcagccagt agacatgctc
    27901 ttgaggttgg aatctattaa tccagtattc attaactaat gcttgaggaa gaatatgctc
    27961 aattgtgatt ctccccgtat aggttttaat cacgctctca tcttgttgct ccatatctaa
    28021 gcgaagcaaa attgctttta ttaagttcac ttgattcgga cgaggctcat ataaatcttc
    28081 atccagagca gctataaaac ctgaattatc tgcatgttgg tttatctgat ttatcacaga
    28141 atcaaaaggc atatcattat ttattgctac cagagcagaa taacaaacca tttctctttg
    28201 gcttttaatt tgttttttta accacccatg catatatacc ttctcaaagg cggtaataaa
    28261 ttgagaaaaa tcatctaaat taaaatcttc tgtacgagcc attcgattca tgaatgccat
    28321 aaccggagga atccactcat ctacccctaa attacttagc gatgctattt ttcgtcgtat
    28381 tgaagggtgt tcaaaatcat tctctaaaat cttagtata
//
LOCUS       DAJTUI010000048        28406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTUI010000048 DAJTUI010000000
VERSION     DAJTUI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.048"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="OO464_002224"
     CDS             complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="OO464_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR6227361.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            323..1954
                     /gene="dcuS"
                     /locus_tag="OO464_002225"
     CDS             323..1954
                     /gene="dcuS"
                     /locus_tag="OO464_002225"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6227362.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1951..2670
                     /gene="dcuR"
                     /locus_tag="OO464_002226"
     CDS             1951..2670
                     /gene="dcuR"
                     /locus_tag="OO464_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6227363.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3241..4581
                     /gene="dcuB"
                     /locus_tag="OO464_002227"
     CDS             3241..4581
                     /gene="dcuB"
                     /locus_tag="OO464_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6227364.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4659..6305
                     /gene="fumB"
                     /locus_tag="OO464_002228"
     CDS             4659..6305
                     /gene="fumB"
                     /locus_tag="OO464_002228"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6227365.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6428..7057
                     /locus_tag="OO464_002229"
     CDS             6428..7057
                     /locus_tag="OO464_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6227366.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7196..8617)
                     /gene="melB"
                     /locus_tag="OO464_002230"
     CDS             complement(7196..8617)
                     /gene="melB"
                     /locus_tag="OO464_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR6227367.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8720..10075)
                     /gene="melA"
                     /locus_tag="OO464_002231"
     CDS             complement(8720..10075)
                     /gene="melA"
                     /locus_tag="OO464_002231"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR6227368.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10358..11266
                     /gene="melR"
                     /locus_tag="OO464_002232"
     CDS             10358..11266
                     /gene="melR"
                     /locus_tag="OO464_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR6227369.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11465..13732
                     /gene="adiA"
                     /locus_tag="OO464_002233"
     CDS             11465..13732
                     /gene="adiA"
                     /locus_tag="OO464_002233"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6227370.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14057..14818
                     /gene="adiY"
                     /locus_tag="OO464_002234"
     CDS             14057..14818
                     /gene="adiY"
                     /locus_tag="OO464_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6227371.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14955..16292
                     /gene="adiC"
                     /locus_tag="OO464_002235"
     CDS             14955..16292
                     /gene="adiC"
                     /locus_tag="OO464_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6227372.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16396..18039
                     /gene="eptA"
                     /locus_tag="OO464_002236"
     CDS             16396..18039
                     /gene="eptA"
                     /locus_tag="OO464_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6227373.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18036..18704
                     /gene="basR"
                     /locus_tag="OO464_002237"
     CDS             18036..18704
                     /gene="basR"
                     /locus_tag="OO464_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6227374.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18705..19805
                     /gene="pmrB"
                     /locus_tag="OO464_002238"
     CDS             18705..19805
                     /gene="pmrB"
                     /locus_tag="OO464_002238"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6227375.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19781..19870)
                     /gene="pmrR"
                     /locus_tag="OO464_002239"
     CDS             complement(19781..19870)
                     /gene="pmrR"
                     /locus_tag="OO464_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6227376.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19982..21484)
                     /gene="proP"
                     /locus_tag="OO464_002240"
     CDS             complement(19982..21484)
                     /gene="proP"
                     /locus_tag="OO464_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6227377.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21748..22626)
                     /locus_tag="OO464_002241"
     CDS             complement(21748..22626)
                     /locus_tag="OO464_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR6227378.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22623..24851)
                     /gene="crfC"
                     /locus_tag="OO464_002242"
     CDS             complement(22623..24851)
                     /gene="crfC"
                     /locus_tag="OO464_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6227379.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25254..25589
                     /locus_tag="OO464_002243"
     CDS             25254..25589
                     /locus_tag="OO464_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6227380.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25747..26190
                     /gene="yjdN"
                     /locus_tag="OO464_002244"
     CDS             25747..26190
                     /gene="yjdN"
                     /locus_tag="OO464_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR6227381.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26323..27111
                     /gene="phnC"
                     /locus_tag="OO464_002245"
     CDS             26323..27111
                     /gene="phnC"
                     /locus_tag="OO464_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6227382.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27136..28152
                     /gene="phnD"
                     /locus_tag="OO464_002246"
     CDS             27136..28152
                     /gene="phnD"
                     /locus_tag="OO464_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6227383.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28258..>28406
                     /locus_tag="OO464_002247"
     CDS             28258..>28406
                     /locus_tag="OO464_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCR6227384.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7297 a         7419 c         6935 g         6755 t
ORIGIN      
        1 acgactttcg tttgagatta aataacttgc cgttgccacg tacgcaattg ccagaatgct
       61 gacatatcgc agtgttgaaa tagacatcga tcttttcgcc ggtataacag cgataacccc
      121 cgtccagtag cgcctgatcc atgaccttcc ctctctcatc agtatgatta tcgctataag
      181 catagccccg gaatcttcat acagcactgg cagtttccgt tgccagagtc tcttccccta
      241 tatagccaat catttttact ccctttgaat tacccgcctc atcagagata atgcttaaga
      301 aatttgtcac acaaggaagc tgatgagaca ttcattgccc taccacatat tacgcaaacg
      361 tccgatgaaa ttgagtacca ctgtgatctt aatggtcagc gcggtactgt tctcggtgct
      421 attggtggtg catctgattt acttctcgca aatcagtgat atgacgcgag acgggctggc
      481 caacaaggca ctggcagtgg cgcgtaccct cgccgactcg ccggaaatcc gtcagggctt
      541 gcagaaaaaa ccgcaggaga gtggcatcca ggccatcgcg gaagccgtgc gcaaacgcaa
      601 cgatctgctg tttattgtcg ttaccgatat gcaaagtctt cgctactcac atcctgaagc
      661 ccagcgtatt ggtcagccat ttaaaggtga tgacatcctt aatgcgctga atggcgaaga
      721 aaatgtcgct atcaatcgcg gttttctggc gcaggcttta cgcgtattta cccccatcta
      781 cgatgaaaat cataaacaaa ttggcgtggt ggcgatcggc cttgagttaa gccgcgtgac
      841 ccaacagatc aatgacagtc gctggagcat tatctggtcg gtattatttg gcatgctggt
      901 cggactgatt ggcacctgca ttctggttaa ggtactgaaa aaaatccttt tcggcctgga
      961 accctacgaa atctccacgc tgtttgagca acgccaggcc atgttgcagt ctatcaaaga
     1021 aggcgtcgtt gccgtggacg atcgcggcga ggtcacgctg atcaacgatg ccgcacaaga
     1081 attgctgaat taccgtaagt cgcaggacga tgagaaactg tcgacgctca gccactcatg
     1141 gtcacaggtg gtagatgtct cggaagtgtt acgcgacggt acgccgcgcc gcgacgaaga
     1201 gattacgatt aaagaccggc tattactgat caacaccgtt ccggtgcgca gtaatggcgt
     1261 tatcatcggt gccatttcaa ccttcaggga caaaactgaa gttcgtaaac tgatgcagcg
     1321 actcgacggt ctggtcaact atgctgacgc acttcgtgaa cgatcccacg aatttatgaa
     1381 taaattgcat gtgattctcg gattattgca tctgaagagt tataagcagt tggaagatta
     1441 cattctcaaa acagccaata actatcagga agagattggc tctctgctgg gtaagatcaa
     1501 atctccggtt atcgctggtt ttttaatcag caagattaac cgcgcgaccg atttaggcca
     1561 tacgctgatt ttaaacagtg aaagccagct gccagacagc ggcagtgagg accaggtcgc
     1621 gacgctgatt accacattgg gaaatctgat agaaaacgcg ctggaagcat tagggccgga
     1681 acccggtggc gaaattagcg taacattgca ctaccgtcac ggctggctgc actgcgaagt
     1741 taacgatgat ggaccgggga tcgcacccga caaaatcgat cacatttttg acaaaggtgt
     1801 ctcgacaaaa ggaagcgagc gaggcgtcgg tttagcactt gtcaaacaac aggtagaaaa
     1861 tctcggcggc agcatcgccg tggaatcgga accggggatt ttcacacaat tttttgtcca
     1921 gataccctgg gacggggaga ggtcgaacag atgatcaatg tattaattat cgatgacgac
     1981 gcaatggtcg cggagctgaa tcgccgatac gtagcacaaa tcccaggctt tcaatgctgt
     2041 ggaacagcct cgacgctgga gaaagccaaa gagattatct tcaatagcga tgcgcctatc
     2101 gacctgatat tgctcgatat ctatatgcaa aaagagaacg ggctcgattt actgcctgtc
     2161 ctgcataacg cgcgttgcaa aagtgatgtg attgtcatct cctccgcagc cgatgcggca
     2221 accattaaag attcgctgca ttacggtgtc gtggattacc tgatcaaacc cttccaggct
     2281 tcccgctttg aagaggcgct caccggctgg cggcaaaaga aaatggcgct ggaaaaacat
     2341 cagtattatg accaggccga gcttgatcag ctaatccacg gcagcagctc caacgaacag
     2401 gatcctcgtc gcttgccaaa aggcttaacg ccgcagacgt tacgcacgct gtgccagtgg
     2461 attgacgcgc atcaggacta tgagttttca accgacgagc tggctaacga ggttaacatc
     2521 tcgcgtgttt cctgccgaaa atacctcatc tggctggtca actgccacat cttgttcacc
     2581 agtatccatt atggcgtcac ggggcgtccg gtttaccgtt accgtattca ggcagaacac
     2641 tactcactgc tgaaacaata ttgccaataa cggatcgctg gttatctgta agtaataatt
     2701 attaccgtaa tgcaaaatac ccctggcaca agttacatcg ttaatcagta aaacttgtgc
     2761 cagatcaaat ataattatcc ctccatcatc gttaaaaatt aatatctctt cagatgaacg
     2821 gtgtttttaa tttcaaaacg ataacaaaag ttaattaact attatgtcac ccgcattatg
     2881 tgtattttta cccacaaatg ggtagatcag attaatctat aaacctaatg atatctgccc
     2941 tgagaacaaa aaatagaccg ataaatatca ataagataac agcaaacaaa acattaacat
     3001 ctgcgcagta caaactataa acccatcgcc agagagtttt tctctctgaa aaagccgctt
     3061 atcacagtgc ataaatttgc cgctgcttta atcagccaat attcactgtg aggtatttgc
     3121 taaagccggt aacgaccaaa cggatattta gtcaggctct gaaaacagtt catacaaaac
     3181 agaacgtgac tgtgatctat tcagcaaaaa tttaaatagg attatcgcga gggttcacac
     3241 atgttattta ctatccaact tatcataata ctaatatgtc tgttttatgg tgccagaaag
     3301 ggtggtatcg cgctgggttt attaggcggt atcggtctgg tcattctggt cttcgtcttc
     3361 caccttcagc caggtaaacc accagttgat gtcatgctgg ttatcattgc ggtggtggcg
     3421 gcatcggcga ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat tgccgagaag
     3481 ctgctgcgcc gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac ctgtacactg
     3541 accattcttt gcggtacggg tcatgtggtt tacaccattc tgccgatcat ctacgacgtc
     3601 gccattaaga acaacatccg tccggaacgt ccgatggcgg caagttctat cggtgcacag
     3661 atggggatta tcgccagtcc ggtgtcggtt gcggtcgtgt ctctggttgc gatgctgggt
     3721 aatgtcacct ttgatggtcg ccatcttgag ttcctcgatc tgctggcaat caccattcca
     3781 tcgacgttaa tcggtatcct ggcgatcggt atcttcagct ggttccgcgg taaagatctg
     3841 gataaagacg aagagttcca gaaattcatc tccgtaccgg aaaaccgtga gtatgtttac
     3901 ggtgataccg cgacgctgct ggataaaaaa ctgccgaaaa gcaactggct ggcaatgtgg
     3961 attttcctcg gggcaatcgc tgtagtcgcc cttcttggtg ctgattcgga cctgcgtcca
     4021 tccttcggcg gcaaaccgct gtcgatggta ctggttattc agatgtttat gctgctgacc
     4081 ggggcgctga ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa aaacgaagtc
     4141 ttccgttccg gtatgatcgc catcgtggcg gtgtacggta tcgcatggat ggcagaaacc
     4201 atgttcggtg cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt gaaagagtat
     4261 ccgtgggcgt atgccattgt tctgctgctg gtttccaagt ttgtaaactc tcaggctgcg
     4321 gcgctggcgg cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc atacatcgtg
     4381 gcttcagcac cggcttgcta cggttattac atcctgccga cttatccgag cgatctggca
     4441 gcgattcagt ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat caaccacagt
     4501 tttattctgc cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg gatcttcgcc
     4561 gcgatgtacg ggttcttata aatgcacttt gcgtgccgcc cgtgactacg cggcacgcca
     4621 ttttcgaata acaaatacag agttacaggc tggaagctat gtcaaacaaa ccctttatct
     4681 accaggcacc tttcccgatg gggaaagaca ataccgaata ctatctactc acttccgatt
     4741 acgttagcgt tgccgacttc gacggcgaaa ccatcctgaa agtggaacca gaagccctga
     4801 ccctgctggc gcagcaagcc tttcacgacg cttctttcat gctacgtccg gcacaccaga
     4861 aacaggttgc ggctattctt cacgatccag aagccagcga aaacgacaag tacgtggcac
     4921 tgcaattctt aagaaactcc gaaatcgccg ccaaaggcgt gctgccgacc tgccaggata
     4981 ccggcaccgc gatcatcgtc ggtaaaaaag gccagcgcgt gtggaccggt ggcggtgatg
     5041 aagaagcgct gtcgaaaggc gtctataaca cctatatcga agataacctg cgctactcgc
     5101 aaaacgcggc gctggacatg tacaaagagg tcaacaccgg cactaacctg cctgcgcaaa
     5161 tcgacctgta cgcggtagat ggcgatgagt acaaattcct ttgcgttgcg aaaggcggcg
     5221 gctctgccaa caaaacgtat ctctaccagg aaaccaaagc cctgctgact cccggcaaac
     5281 tgaaaaactt cctcgtcgag aaaatgcgta ccctcggtac tgcagcctgc ccgccgtacc
     5341 atatcgcgtt tgtgattggc ggtacgtctg cggaaaacaa cctgaaaacc gtcaaattag
     5401 caagcgctca ctattacgat gaactgccga cggaagggaa cgaacatggt caggcgttcc
     5461 gcgatgtcca gctggaacag gaactgctgg aagaggccca gaaactcggt cttggcgcgc
     5521 agtttggcgg taaatacttc gcgcacgaca ttcgcgttat ccgtctgcca cgtcacggcg
     5581 catcctgccc ggtaggcatg ggcgtctcct gctccgctga ccgtaacatt aaagcgaaaa
     5641 tcaaccgcga aggtatctgg atcgaaaaac tggaacacaa tctaggtcag tacattccac
     5701 aagaactgcg tcaggccggt gaaggcgaag cggtgaaggt cgaccttaac cgcccgatga
     5761 aagagatcct cgcccagctt tcgcaatatc cggtttccac tcgtttgtcg ctcactggca
     5821 ccattattgt gggtcgagat attgcacacg ccaagctgaa agagctgatt gacgccggta
     5881 aagaacttcc gcagtacatc aaagatcacc cgatctacta cgcgggtccg gcgaaaaccc
     5941 ctgccggtta tccatcaggt tcacttggcc caaccaccgc aggtcgtatg gactcctatg
     6001 tggatctgct gcaatcccac ggcggcagca tgatcatgct ggcgaaaggc aaccgcagcc
     6061 agcaggttac cgacgcgtgt cataaacacg gcggcttcta cctcggtagc atcggcggtc
     6121 cggcggcggt actggcgcag cagagcatca agcatctgga atgcgtcgct tatccggagc
     6181 tgggtatgga agctatctgg aaaatcgaag tagaagattt cccggcgttt atcctggtcg
     6241 atgacaaagg taacgacttc ttccagcaaa tcgtcaacaa acagtgcgcg aactgcacta
     6301 agtaacctct tcggcccagc gcctggcagc atgctgccag gtgatccccc tgggccacct
     6361 cttttgcgat tgtaatttca tgcttgctgg tgaatagtca gtattttccc tgacttgcga
     6421 actcaccatg acccgcacac tcaagccgtt aattcttaac actggcgcac tggcgctaac
     6481 gttaatcctg atttataccg gcatttcggc ccatgacaaa ctcacctggc tgctggaagt
     6541 gacaccggtg attatcgtcg tgccgctact acttgccacc gccagacgtt acccgttaac
     6601 gctgctgctc tatacgctca tcttctttca cgccatcatc ctgatggtag gcgggcagta
     6661 cacctacgcg aaagtccccg ttggttttga ggtgcaggaa tggctggggt tgagtcgtaa
     6721 tccgtatgac aagctggggc actttttcca ggggctggtg cctgcactgg tggcacgaga
     6781 aattctcgtt cgcgggatgt acgtgcgcgg acgtaaaatg gtggcgtttc tggtctgttg
     6841 cgtggcgctg gcgataagcg cgatgtatga attaatcgag tggtgggcgg cactggcgat
     6901 gggccaggga gcggatgatt ttctcggtac ccagggcgac cagtgggaca cgcaatccga
     6961 tatgttctgc gcgctgcttg gcgcattaac gacagtgata ttgctcgctc gctttcattg
     7021 ccgccagcta cggcgctatg gcttgataac tggataacaa cgtgcacttt gttgatgccg
     7081 gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc attacattaa
     7141 gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt ccacgtcagg
     7201 ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg ggcggtactt
     7261 tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca ttgaggcgat
     7321 agaaacggaa gtagagaatc agcgttacca tgaaaaacag agtcggtaga gcaatcataa
     7381 taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca tagccaatca
     7441 tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc accaccatag
     7501 tctgcacgga gtaagcgatg ctttcacagc gtacgtgcag tttatattca ccgtaatcaa
     7561 cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc acattcagca
     7621 aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc attaacagga
     7681 gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg cgtcgggata
     7741 atgatttaac taagcgtggg aagaatacta acgtcaccag gttagcagct cccgcatacg
     7801 acagataata ggggaacaaa tccgcatcac cgataacata tgagaaataa tagatagcaa
     7861 atccggtaat aatgttgctg gctacattat aagcaagagc catacccaag aggcatgaaa
     7921 gctgatcgtt tttataaata agcgcaacga tggctttaag tgtcagatgg cttccttcag
     7981 cagacgattg attgtctgac gaaaagactt catgcacatt gcgcagagtg atgatggttg
     8041 aaacaataaa aaaggcgatc aataccagag tgaacatctg aaagccaaat ccccgatcgc
     8101 caccgccgac ataattaaca aatggtagcg tcacacctgc cgtaacaaag cctgccagac
     8161 tggcaaaaaa acgcggataa ggaaccagtt gttcgcgttc acgtttatcg agcgtgatgg
     8221 ttggaaccag cgaccagaag ggaatatcca taatggtgta agtcatgccc cagaggatgt
     8281 aggtcacgca aacaaagaca atctgagtag taccttcaaa cagatgcgca ctaaagagga
     8341 gaaataagat tacagagttt gccaacgtac cgatcaggat ccagggtttg aacttacccc
     8401 atcgcgatcg cgtagcattt acaatccatc ccataatcgg atcgttaata gcatcccaga
     8461 tcctcgccac cagaaacaaa gtaccgacca aacccacaga cagcccgacg acatcggtgt
     8521 agtaatacat gaggtacata tacacaatgc cgatcgcaaa atccttcccg aacgctccaa
     8581 atccataact gagttttgta gtcattgaaa tgctcatagg gtatcgggtc gctgttacca
     8641 acgcccgcct tctgttattg gtatcgtcag aggtgtgcgc cggaataaat cccccggcgc
     8701 agtagcgttt agtcgcgttt taacggtgca accagcttgg cagccagtcg ccgtgggcgg
     8761 caatcaggtc gtcaacaaga gcatatattt cgtcaatgcc cagcacggcg gcagtatgcg
     8821 ggtccatcat cgcggcgtgg taaacacggt cgcgattttc cgtaagaata gcttcggtca
     8881 gcagcgtctg tacgttgatg ttggtttgca tcagggcggc cagatgcgaa ggtagcgtac
     8941 cgactttggt cggctgaatg ccattagcat caaccagaca ggctacttcc acgcaacatc
     9001 cttgtggcag gttatcaatc aaaccatcgt tacggacgtt gccgtaaatc acactcggct
     9061 cgccagtcca gatagcgttc atgattgtgc tggcatattc ccgtgacggt ttaatatcaa
     9121 tccgggaggc gtttttatac tcctccagct ctttatgcca gttcgccagc tgctcgacgc
     9181 agcgtttcgg gtactcatcc agcggtactt tataacgctc aatcaaatcc tcacgaccta
     9241 gcttaataaa ccacggcgta tattcggcaa aatgttctga tgattccgtg acgaagtagc
     9301 ccagcttttt gaacatttcg tagcgcacaa tattctggca gcgagtatta ccatgaatat
     9361 ttggcttcgg tgcctgccct gcttcataag ccgccagcag ttccgggtag agattcacgt
     9421 aactgccgtc ggcggttttg cgctccagct ccaggtaaaa cgccatatgg ttgatacctg
     9481 cgcaacggta acgcagcgta gccgggtcga tattgaggtc acgcgccagc tcttccgccg
     9541 ttccctgcac cgaatggcac agcccgacct gtttgatata cggatagcgg gcatacatcg
     9601 cccaggtatt catcgccatt gggttaacat agttgagcat ggtggcatcg gggcagactt
     9661 ccgtcatgtt ctcgcaaatt tgccacagat gcggaatggt acgtagcgcg cgcataatac
     9721 cgcccggccc caacgtatcg gcaatggttt gttccagacc atgccgctta cagacctcga
     9781 aatcagtcac cgtgcaaggt tcataaccgc caatctgaaa tgccaccacg acaaaatcgg
     9841 catcctctaa ggcttctttc tgttgggtgt ggcaggtgat tttgccgctg gcccctgctg
     9901 aatccatcag cttacgcacc acaatatgcg actcttccag gcgggtgggg tcaatgtcca
     9961 tcagggcaat atgcgccgtt ttcagcgcct cgcgatggaa cacatcacca agaatatttt
    10021 taacgaaaat cgtcgaacca gcgccgataa atgtaatttt gggtgcagac atcatgcaga
    10081 tctcctggct tgcttgaata acttcatcat ggcaggcgaa tctgcagaaa acctccggct
    10141 tccaggttga gcattcccat aaactcagat cctgcgtgaa gcagcagtaa atctgagttt
    10201 atgggaataa attacctgaa aagtagagtt ttcggcctca tgatgctcgg acagtcccct
    10261 cgcccctctg ggaagagggt gaaaaccgtg ctcccactcg cagtcatcct ccctcactcc
    10321 tgccataatt ctgatattct aggaaagaga gccatccatg aatacagata cgtttatgtg
    10381 cagcagcgac gaaaaacaga cccgcagccc gctgtcgctg tattcggaat accagcggat
    10441 ggagattgag tttcgcgcac cgcatatcat gcccaccagc cactggcatg gtcaggtcga
    10501 agtgaatgtg cctttcgatg gcgatgtgga atacctgatc aacaatgaaa aagtgaatat
    10561 caatcagggt catatcacgc tgttctgggc ctgtacaccg caccaactaa cagataccgg
    10621 aacctgtcag agcatggcga tttttaatct gccgatgcat ctgtttctct cctggccgct
    10681 ggataaagac ctgattaacc acgtcactca cggcatggtg atcaaatcac ttgccacaca
    10741 gcaacttagc ccgtttgaag tacgccgctg gcagcaggaa ttaaacagtc cgaatgagca
    10801 aattcgccag ctcgccattg atgaaattgg cctgatgctc aagcgattta gcctctctag
    10861 ctgggaaccg attctggtca ataaaacctc gcgcacacac aaaaacagcg tctcgcgcca
    10921 tgcgcaattt tatgttagcc agatgctggg ctttattgcc gaaaactatg atcaggcgct
    10981 gaccatcaac gatgtggctg agcacgtcaa acttaacgcc aactatgcaa tggggatatt
    11041 ccagcgggtc atgcaattga cgatgaaaca gtacattacc gcgatgcgca tcaaccacgt
    11101 tcgcgcgtta ctgagcgata ccgataaaag tattctcgat attgccctga cggcaggctt
    11161 tcgttcaagt agtcgttttt acagcacgtt cggcaaatat gtcggcatgt cgccgcaaca
    11221 ataccgcaaa cttagccaac agcgccgcca gacgtttccc ggctaaagac gatatcagta
    11281 tcagccaaaa aaatagttta gccgtcgata ataagcagtg aaaataattt aaaataatca
    11341 cataacgtat tgtttataaa actaaatatt tcaaaaatgt ttgtttttca cgcgctttac
    11401 agcccgaaaa ggccggaaga tacttgcccg caacgaagat tccttcataa ccgggtaagc
    11461 aatgatgaaa gtattaattg ttgaaagcga gtttctccat caagacacct gggtcggtaa
    11521 cgccgttgag cgtctggcag atgctttaag ccagcaaaat gttaccgtga ttaaatccac
    11581 ctcctttgat gatggttttg ccattctctc ttcaaacgaa gccattgact gcctgatgtt
    11641 cagctatcaa atggagcatc cggacgaaca tcaaaacgtc agacaattga tcggtaagct
    11701 tcatgagcgc caacaaaacg tgccggtctt cctgttgggc gatcgggaaa aagccctcgc
    11761 cgcaatggac cgggatctgc tggagcttgt cgatgaattc gcctggattc tggaagatac
    11821 cgccgacttt atcgccggac gcgccgttgc cgcgatgacc cgctaccgcc agcagctgtt
    11881 gccgccactg ttcagcgcgc tgatgaaata tagtgacatc catgaatatt cctgggcagc
    11941 gccaggccac cagggcggcg ttggttttac caaaacaccc gccggacgtt tctaccatga
    12001 ctactatggt gaaaatctgt tccgtaccga catgggcatc gaacgaactt ccctcggttc
    12061 tttgcttgac catactggcg catttggcga aagcgaaaaa tatgccgcac gcgtatttgg
    12121 tgccgatcgc tcctggtcgg tagtcgtcgg tacttccggc tctaaccgca ccatcatgca
    12181 ggcttgcatg accgataacg atgtcgtggt cgttgaccgt aactgccata aatccatcga
    12241 acaaggtttg atgctgacag gcgcgaaacc ggtctatatg gtgccaagcc gcaaccgcta
    12301 cggcattatc gggccaatct atccgcagga aatgcaacct gaaaccttgc aaaagaaaat
    12361 cagtgaaagc ccgctgacca aagacaaagc cgggcaaaaa ccgtcttact gcgtggtaac
    12421 caactgcacc tatgacggcg tgtgttataa cgctaaagaa gcgcaggatc tgctggaaaa
    12481 aacctccgat cgtctgcact ttgacgaagc ctggtacggc tatgcacgtt tcaacccgat
    12541 ctatgccgat cactatgcca tgcgcggcga acctggcgat cacaacggtc ctaccgtttt
    12601 cgccacccac tccatccaca aactgctgaa tgcgctgtca caggcttctt atattcatgt
    12661 acgtgaaggt cgtggggcga ttaacttctc ccgcttcaac caggcctaca tgatgcatgc
    12721 caccacctcc ccgctgtatg ccatctgcgc atccaacgac gtggcggtgt cgatgatgga
    12781 cggcaacagc ggcctgtcac tgacacagga agtgattgac gaagcggttg atttccgtca
    12841 ggcgatggcg cggctatata aagagttcac cgctgacggt agctggttct tcaaaccgtg
    12901 gaacaaagaa gtcgtcaccg acccacaaac cggcaaaacc tatgactttg ctgacgcacc
    12961 aaccaaactg ctgaccaccg ttcaggactg ctgggtaatg catccgggcg aaagctggca
    13021 cggcttcaaa gatattccgg ataactggag tatgctcgac ccaattaaag tcagcatcct
    13081 tgctccggga atgggtgaag atggtgaact ggaagaaacc ggtgttccgg cggcgctggt
    13141 cactgcctgg cttggtcgcc acggcattgt gcctacccgc accactgact tccaaattat
    13201 gttcctgttc tctatgggcg taacccgtgg gaaatggggg actctggtta acaccctttg
    13261 ctccttcaaa caccactatg acgccaacac accgctggcg caggtgatgc cggaacttgt
    13321 tgaacaatat cctgacactt acgcgaacat ggggattcac gatctgggtg acaccatgtt
    13381 tgcctggctg aaagaaaaca accctggcgc acggttgaac gaagcctatt ccggcctgcc
    13441 ggtggcggaa atcaccccgc gtgaagcgta caacgcgatt gtcgacaaca atgtcgaact
    13501 ggtatccatt gaaaatctgc caggacgcat cgcggcaaac tcagttatcc cgtatccgcc
    13561 aggaatcccg atgctgctgt ctggtgaaaa cttcggcgat aaaaacagtc cgcaagtaag
    13621 ttatttacgc tcgctgcaat cctgggacca ccatttccct agatttgaac acgaaactga
    13681 agggactgaa attattgacg gtatttacca cgttatgtgc gtgaaagcgt aaccactatt
    13741 ccgctgaagg cgtaattgtt taaataacat tacgccgcct ggccttaggc cttttgagta
    13801 tggcaacgtt ttcataaaaa ttgctgcaaa caaaaatgtc atactttttg cgcggcccca
    13861 ccccacgctt ttttttgcct gttatttatc ctgtaaaaaa atatgtacat gagaaattac
    13921 tataaaaatt tgtaatatta gtaaaactcg ttatttttat gcatgtttat attcatcatg
    13981 caattatata accatttccc ggtatcgctt tgctttagcg agaactggtc ttttattcgc
    14041 actcaggagt acatgtatga ggatttgcag cgaccaacct tgtattgttt tattgactga
    14101 aaaagatgtc tggataaggg tgaatgggaa agaacctatt agccttaaag ctaaccatat
    14161 ggcgttatta aattgtgaaa ataatattat cgacgtctcc tctcttaaca acactttggt
    14221 tgctcatatt agtcacgaca tcatcaaaga ttacctccgg tttctgaata aagatctctc
    14281 gcaaatacca gtatggcaac gtagcgctac gcccatactc accctgccat gcctgacgcc
    14341 agacgtcttt cgcgttgccg cgcaacacag catgatgccc gcagaaactg agtcagaaaa
    14401 ggaacgaaca cgcgcattat tattcacggt gctatcccgt tttctcgaca gtaaaaaatt
    14461 cctttcatta atgatgtata tgttacgtaa ttgtgtaagt gacagcgttt atcaaattat
    14521 tgaaagcgat attcacaaag actggaatct tagtatggta gccagttgtt tatgtcttag
    14581 cccaagtctg ttaaagaaaa agttgaaaag cgaaaacacc agttatagcc aaataatcac
    14641 cacctgccgc atgcgttatg ccgtaaatga attaatgatg gacggtaaaa atatctctca
    14701 ggtatcacag tcctgcggct acaacagtac gtcgtacttt atttctgtct ttaaagactt
    14761 ctacggtatg acgccgctgc attatgttag tcagcacaga gaacgcactg tcgcctgatt
    14821 tttaacctta acgaagagct atattaataa cggcatcagc gataacccgg tcgataataa
    14881 ttcaactatc gaatgcaggc gtatgatatg acgtaattta ttgtcacgaa gctcgccttc
    14941 gcaggagttt aattatgtct tcggatgctg atgctcacaa agtgggctta atccccgtca
    15001 ccctgatggt gtcggggaat attatggggt caggtgtttt tctgttacct gcaaacctgg
    15061 cctccactgg cgggattgcc atttatggat ggttggtgac gattatcggt gcgctggggc
    15121 tctcgatggt atacgccaaa atgtcgttcc tcgacccaag tcctggtggt tcttacgctt
    15181 acgcccgccg ctgctttggc ccgtttctcg gttatcaaac caacgtcctc tactggctgg
    15241 cctgctggat cggcaatatc gccatggtgg tcattggcgt aggatattta agttacttct
    15301 tcccgattct gaaagatcca ttggtattaa ccatcacctg cgtcgtggtg ctgtggatct
    15361 tcgtcctgct gaacattgtc ggtccgaaaa tgatcacccg cgtgcaggcc gttgccactg
    15421 tgctggcgct gatccccatc gtcgggattg ccgtatttgg ctggttctgg ttccgtggtg
    15481 aaacctatat ggcggcatgg aacgtcagcg gcctgggcac cttcggtgcg attcaaagta
    15541 cccttaacgt tacgctgtgg tcgttcatcg gtgtggaaag tgcctccgtt gccgcaggtg
    15601 tggtgaaaaa cccgaaacgc aatgtcccta tcgccaccat tggtggggta ttgattgccg
    15661 ccgtttgcta tgtgctctcc accaccgcga ttatggggat gatccctaat gccgcactgc
    15721 gcgtttctgc ctcgccattc ggtgatgccg cacggatggc gttgggtgac accgctgggg
    15781 ccattgtttc cttctgcgca gctgcgggtt gcttaggttc actgggcggc tggacgttgc
    15841 tggcgggtca aacggcgaaa gccgctgccg atgacggact gttcccaccg atttttgccc
    15901 gtgtaaataa agcgggtacg ccagtggcgg ggctgattat cgtcggtatt ttgatgacca
    15961 tcttccagct cagcagcatt tcaccaaacg cgaccaaaga gttcggtctg gtttcttccg
    16021 tctcggtcat ctttacactg gtgccatatc tttacacctg tgcggcgtta ctgctgctcg
    16081 gccacggtca ctttggtaaa gcacgcccgg catatctggc tgttactacc attgccttcc
    16141 tctactgcat ttgggccgtg gtggggtccg gggcgaaaga ggttatgtgg tcatttgtca
    16201 ccctgatggt catcaccgcc atgtatgcgc tgaattacaa ccggctacat aaaaacccgt
    16261 atcccttaga tgcaccaata agcaaagatt aattcccctt aatccagcaa acataaaagc
    16321 caaccttaag aacttaaggt tggcttaatt ttgctttgcg agcatatgcg cactttgttc
    16381 gatggaaaca ccgtgatgtt gaagcgccta ctaaaaagac cctctttgaa tttactcgcc
    16441 tggctattgt tggccgcttt ttatatctct atctgcctga atattgcctt ttttaaacag
    16501 gtgttgcagg cgctgccgct ggattcgctg cataacgtac tggttttctt gtcgatgccg
    16561 gttgtcgctt tcagcgtgat taatattgtc ctgacactaa gctctttctt atggcttaat
    16621 cgaccactgg cctgcctgtt tattctgatt ggcgcggctg cacaatattt cataatgact
    16681 tacggcatcg tcatcgaccg ctcgatgatt gccaatatta ttgataccac tccagcagaa
    16741 agttatgcgc tgatgacacc gcaaatgtta ttaacgctgg gattcagcgg cgtgcttgct
    16801 gagctgattg cctgctggct aaaaatcaaa cctgccacct cgcgtctgcg cagtgttctt
    16861 ttccgtggag ccaatattct ggtttctgta ctgctgattt tgctggtcgc cgcactgttt
    16921 tataaagact acgcctcgtt gttccgcaat aacaaagagc tggtgaaatc cttaagcccc
    16981 tctaacagca ttgttgccag ctggtcatgg tactcccatc agcgactggc aaatctgccg
    17041 ctggtgcgaa ttggtgaaga cgcgcaccgc aacccgttaa tgcagaacga aaaacgtaaa
    17101 aatttgacca tcctgattgt cggcgaaacc tcgcgggcgg agaacttctc cctcaacggc
    17161 tacccgcgtg aaactaaccc gcggctggcg aaagataacg tggtctattt ccctaatacc
    17221 gcatcttgcg gcacggcaac ggcagtttca gtaccgtgca tgttctcgga tatgccgcgt
    17281 gagcactaca aagaagagct ggcacagcac caggaaggcg tgctggatat cattcagcga
    17341 gcgggcatca acgtgctgtg gaatgacaac gatggcggct gtaaaggtgc ctgcgaccgc
    17401 gtgcctcacc agaacgtcac cgcgctgaat ctgcctgatc agtgcatcaa cggcgaatgc
    17461 tatgacgaag tgctgttcca cgggctggaa gagtacatca ataacctgca gggtgatggc
    17521 gtgattgtct tacacaccat cggcagccac ggtccgacct attacaaccg ctatccgccg
    17581 cagttcagga aatttacccc aacctgcgac accaatgaga tccagacctg taccaaagag
    17641 caactggtga acacttacga caacacgctg gtttacgtcg actatattgt tgataaagcg
    17701 attaatctgc ttaaagaaca tcaggataaa tttaccacca gcctggttta tctttctgac
    17761 cacggtgaat cgttaggtga aaatggcatc tatctgcacg gtctgcctta tgccatcgcc
    17821 ccggatagcc aaaaacaggt gccaatgctg ctgtggctgt cggaggatta tcaaaagcgg
    17881 tatcaggttg accagaactg cctgcaaaaa caggcgcaaa cgcaacacta ttcacaagac
    17941 aatttattct ccacgctatt gggattaact ggcgttgaga cgaagtatta ccaggccgcg
    18001 gatgatattc tgcaaacttg caggagagtg agtaaatgaa aattctgatt gttgaagacg
    18061 atacgctgtt attgcaggga ctgattctgg cggcgcaaac cgaaggctac gcgtgcgatg
    18121 gcgtgacaac cgcgcggatg gcggaacaaa gccttgagga cggtcattac agcctggtgg
    18181 tactggattt agggttaccc gacgaagatg gactgcattt tctcgcccgt atccggcaga
    18241 aaaaatacac cctgccggta ctgatcctca ccgctcgcga tacgctgacc gacaaaatcg
    18301 ccgggctgga tgtcggtgcc gatgactatc tggtgaagcc ttttgcgctg gaagagttac
    18361 acgcccgtat ccgcgccctg ctacgacgcc ataataatca gggcgaaagt gagctgattg
    18421 ttggcaatct gacgctgaat atgggtcgcc gtcaggtatg gatgggcggt gaagagttga
    18481 ttctgacgcc caaagaatat gctctgctgt cacggttaat gctcaaagca ggcagtccgg
    18541 tacatcggga aattctctac aacgacatct ataactggga caatgaaccc tcgaccaaca
    18601 ccctggaagt gcatatccac aatctgcgcg acaaagtggg caaagcccgt atccgcaccg
    18661 tgcgcggctt tggctatatg ctggtcgcga atgaggaaaa ctaattgaat ctgatgcgtt
    18721 ttctgcgccg acctatatcg ctgcgccaac ggctgatatt gaccatcggg gccattttgt
    18781 tggtgtttga gctgatcagc gtcttctggc tatggcatga aagtaccgag cagattcagc
    18841 tgtttgagca ggcgctacgc gacaatcgca acaacgatcg ccacatcatg cgtgagatcc
    18901 gcgaggcggt cgccagcctg attgtccccg gcgtctttat ggtcagcctg acgctattta
    18961 tctgctatca ggcggtacgc cgcatcaccc gcccgctggc ggagctgcaa aaagagctgg
    19021 aagcgcgcac cgccgacaac ttaacgccca ttgccattca cagcgccacc ctcgaaatcg
    19081 aagcggtggt ttcggcgttg aacgatctgg tcagtcgcct gaccagcacg ctggataacg
    19141 aaaggttgtt taccgctgac gtcgcgcacg aactgcgaac gccactggcg ggggtgcgtt
    19201 tgcatctgga actgctggcg aaaacgcatc acattgatgt agcaccgtta gtggcacggc
    19261 ttgatcagat gatggagagc gtctcccagc tgctgcaact ggcgcgtgcc ggacagtcat
    19321 tttcttccgg taattatcaa catgtaaaac tgctggaaga tgtcattctc ccctcgtatg
    19381 acgaactcag taccatgctc gaccagcgac agcaaaccct gctattgcca gagagcgccg
    19441 ccgacatcac cgttcagggt gatgcaaccc tgctgcggat gttattgcga aacctggtag
    19501 aaaacgccca tcgttacagc ccgcaaggca gcaacattat gattaagctg caagaagatg
    19561 gcggagcggt catggcagtt gaagatgaag gaccaggtat tgatgagagt aaatgcgggg
    19621 agttgagtaa agcgtttgta cgtatggaca gccgttatgg cgggattggt ctggggttaa
    19681 gtattgtcag ccgcattaca cagttgcatc acgggcagtt tttcctgcaa aaccggcaag
    19741 agacttccgg cacgcgggcc tgggtacggc tgaagaaaga tcagaacgtg gcaaaccaga
    19801 tataaagaaa gctgctgacc accagcacgc tgaacacggt agttaaactt tcataaacac
    19861 ggtttttcat tgcactctcc tccgttaacc tggaggagag tatgcgcgtc agagattaag
    19921 tgaaccttaa gagttcaatt ggcctgataa gacagcgtca catcaggcca tccgtttcag
    19981 cttattcatc aattcgcgga tgttgctgca ccaggcgggt acgtttcgcc tgcaaatcgg
    20041 caatctcgtg gtcaatatta tcgattttct gctcgatatt atcgtaatgc tcgacgagaa
    20101 tttccttcgc ttcctgtata tctgacgccg ccggtgtcgc acctttcaac ggacgatttg
    20161 ccgtctcttt catggttacg ccggtgatta aaccaatcac cgccactacc atcaggtaat
    20221 aggcaggcat catcagattc tgcgagcttt cgaccagcca ggccgccagt gttggcgtca
    20281 gaccggcaac cagcaccgaa atattaaatg ccgccgccag cgcgctgtaa cggatatgcg
    20341 tcgggaacat cgctggcaag gtagaagcca taacgcccat aaagcagtta aggatcaccg
    20401 ccagcatcag taacccggca aaaatcaggc cgatgacgtt actgttaatc agaataaacg
    20461 ccgggatcgc caacacaaac agtgcaacac taccaagtag cacaaacgga cgacggccaa
    20521 aacggtcact cagcaagccc atcaccggct ggacaaacag cataccgatc ataatggcga
    20581 taataatcag caccccgtgg tcttcggagt aatgcaggtt atgcgacaaa taactcggca
    20641 tataggtcag caacatgtag taagtcacgt tggtggcaat taccagacca atacatgtca
    20701 acaggctgcg ccagtattta gtggcaatct ctttaaacga gactttcggg ccatcctgca
    20761 aaccttcgcg gtcgccctgt tccagtttat caacatgctg ctggaacgcc ggagtttctt
    20821 ccagcgcatg gcgcaggtaa agcccgataa tccctaacgg cagagcaata aagaacggaa
    20881 tacgccagcc ccagtcgagg aagttcgctt cgccgacaat ggtcgaaatt aacaccacca
    20941 cgcccgcacc cagcacaaac ccggcaatag aaccgaagtc cagccagctg cccataaagc
    21001 cacgtttacg gtcaggggag tattccgcaa caaatatcga cgccccggta tattcaccac
    21061 cgaccgagaa accttgtgcc atcttacaga tcaacagcag aatcggtgcc caaataccaa
    21121 tcgtgtcgta ggacggtatt aagccaatac agaacgtact gatcgacata atcacaatag
    21181 tgatagcgag gatcttctgg cgaccatatt tatcgcccaa cataccaaag aagagtccgc
    21241 caagcggtcg aatcagaaag ggaacggaga aagtggcaag tgcagcaacc atctgcacgc
    21301 tggggtcagc ccccgggaaa aaaactttac ctaatgcgta agcaacaaaa ccataaacac
    21361 caaaatcaaa ccattccatt gcattaccca gtgatgctgc ggtaatggct ttacgcagtt
    21421 taccgtcatc aataatggtg acatcacgaa gggtaatcgg ttttactttt ttccttttca
    21481 gcatagcttt cctcgcagag ttggcccatt tccgcaaacg ccagaaaaat cagcgcgacg
    21541 gccgcaaaag gggagcggga attgcaggat gcaatctctg taacaagcgt agcgggtttg
    21601 cttacaccct ccggttaagg aatgtacaat caaaccagtt gacgcctgaa ttgggcagtt
    21661 aatgaccttt ttaccctacc agtgtttaaa tttgtgatca acttcacaca tggatttcgt
    21721 ttaggactca ttgatgtaac tgattattta cgccaaattt acagagaaga tttcctcgac
    21781 cattgccccc accaaacgct gcgctgaaca gagtcgtggc atccccagcg cgacagtttg
    21841 ccagcgttgg gtcactgacc agcaaacggg ttgtttatct gccgtggtcc actttcccag
    21901 ccaacttaaa aaatcagcat gatccccgcc cgtcggaacg gtcgaagagg caaaccactg
    21961 atggtaatag tgacgcgtag tcggtgccgc attgatactc tctgccagat gttgtgtcgc
    22021 tttctcgcgc aaatcaccca gcaacaacac caccgcttgc gggcttccca gacggcaggc
    22081 atcgccaaat gcaccccagc gcaactcctc cagaacgata aaacagcgcc caggcaacga
    22141 ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt tgctcgctct gatcacctgc
    22201 ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc cagcgacaac gggctgaaaa
    22261 ccactcatac aaggcggcaa tttgctgggg gagctccggg aaagcagagt gctggcggtg
    22321 atccattttt tgcagcagcg ccagatgctg cgcgatctgc gtcagcgcca gatgaccggg
    22381 atccagcatc gacgccagtt tttccaccat tgtcagtcgc aggctaagcg gcgcatttaa
    22441 catgccgccg ttcgtccatg tctgaagctg cacacgcgaa agaagttcct gcatcagtcg
    22501 ttcacgaaac tgctgctgat gggcttgagg atggcgggca tcatcgccct gcgccagatc
    22561 cactaaaaag cgcggatgaa ccgactccag cacgcgaccg gggccgtcca gtaacgtctt
    22621 ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat cgcgtaacag ctgagcgtcg
    22681 gtgtaaatcc agctgtgagt catcacagtc tgctgcaatt gctgctgcat cagcctgacc
    22741 accgattcat tttgttgacg cagagccaga ctttcgcgta aacgcgtctg taattctgtc
    22801 aaacatgaag cgaactcagc gaaaaaagta ttcatgcctg ccgtaacaga ttcatcgacc
    22861 tgctctgcca gaactttagc catttgttgg caatagagat cgacttctgc gcttaatgct
    22921 cgttgcaaca cactgtaatc aaccgtttct gtcggggatt tctcatttcc ccgtccccag
    22981 tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa cgcctaaacg cgtgctctgc
    23041 tcgtccgttg cttcctggcg cgaaatgact tcactgaaca gctggcgagt gttgaagtgg
    23101 ggaactacgc cgtgaaaaac aggaaaatga aacccaggac gaaaccctga ctcgctcaat
    23161 tccattttga cttgttgctc aatggggcga ataacatcgg ttaacacccg gcaaagggtg
    23221 gattccatct cggcaaaacg cagcgtaaag tcgcgactga tggtgttctg cgccgtctgt
    23281 aacagtgtct cacagcgggt acgaatctcg cttaacggct ccgaatcatc ctgaaacaag
    23341 gcggctaact gcgcattcac cgcatcttgc tgttgacgca gaaagtggtt ggcggaggtc
    23401 agggccagct cgatttcatg tttaatctcg ccgctcacct gcgcctgatt gagttgcaat
    23461 agctgcaaac tttcttcgac ctgatggata ttttgccgca attgttcaca agcgacgttt
    23521 aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct gcgcgtaatt caacagttta
    23581 tgcgcagcag atcgcagagc atacaatgag gcgttagcgt aagcggcatg aagcaacgcc
    23641 tgaattggct gggcgaacag cgaatcttcc cacaactgat cggcagcatg acgaatatgt
    23701 tcgaggtccg ccagatcggc atgacgccag cgcctgccga gcgcggcatg ggcaaaatct
    23761 tccacccagc gttgttgctc tggcgctggt aacttaccgt tgttggctaa ctcatagcgc
    23821 gcccgattcg ccaggtagcc ccacatcgac gacaccggga atatctgctg tggcgtaata
    23881 cagcctttca tcagcgtccc ggaaatcagt gcccgcacct ggtcggcgtc gtcactgtta
    23941 cgatcctgtt gatcgaactt attgaccagc acatacagcg gcaccgattg ccccaccgcc
    24001 aaaatcgcct cacggacctc ttcatcggag atcgatttca gttgcgtata atccagcacc
    24061 gccagtaccg ccgaggcgcg tgccagctgc tggttaagca ttttttgcag atgcggttgc
    24121 ccggcttcat ttggcccggg ggtatccagt aacgtcaact gaccgggata actttccagc
    24181 cccgccagat ggacaaactc cacttcaatc acgggaatat gctcaatggc ggcgtaagca
    24241 gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca ctaaatcatt caaacttttc
    24301 agacaatgaa aaataggctg ggcacccaga taatattttt cgaaagcgac gccattttcg
    24361 atccgctgca taagcgcacg catatcttta tctatttcca gcacatcggt cagatgctta
    24421 atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta aacaatcgat tggcgcgaca
    24481 tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat ggcgaataag cgtcggcagc
    24541 gcagtcattg ggcgattacg attaggcaga acctccgtac caacaatggc attaatggtg
    24601 gttgatttcc ctgctttcat ggtaccgaca attgccagca ccatttccag tcgggaaatt
    24661 ttacacaact cattattcag catcgcgtga cgttcggcga tattaggctg actccagggt
    24721 aaagccagtt gtggcgcgtc gtctccgggt acagagagag gcattttttc cagtaactgc
    24781 aactgttggc gagaaagctg taacaggcgt tcagcctcct gacttaactc atacagggtc
    24841 tgtgtgtaca tagaaaattc ttccttaaag caaattttgt tacttttatt tagccagatt
    24901 gtttttgagt tctgttttcg gcttttataa ttactgcaag aaataatttt atatttagtg
    24961 tgttgttttt ttatcagaat aaataacgtc ttctgatgcg tttaaaacgt cagaaagata
    25021 aaaatatcat gtgaataaaa aaaagaacaa gtagagcatt aacattatct taaataataa
    25081 atagaggcaa aaagattatt ttctttttgc gtttcctttc aaatgaaaac gatcgtcgtc
    25141 taaaatcagc agtacccccg acaaactcag ggattttgtg tataattgcg gcctttttcg
    25201 gcaatctgcc gttttttggc gcttttgccc tgctgacttt tgaggaaatc cacatgtcat
    25261 taccacactg cccaaaatgc aactccgaat acacttacga agataacggc atgtacatct
    25321 gcccggaatg tgcctacgaa tggaacgacg cagaacctgc acaggaaagc gacgagctga
    25381 tcgttaaaga tgctaacggc aatctgctgg ctgacggcga cagcgttacc atcattaaag
    25441 atctgaaggt gaaaggtagc tcttcgatgc tgaaaattgg caccaaagtg aaaaacatcc
    25501 gcctggttga aggcgaccat aacatcgatt gcaaaatcga cggttttggt ccgatgaaac
    25561 tgaaatctga gtttgtgaaa aagaactgat tgtattgtga tcggtaagcc ggataaggcg
    25621 ctcgcgccgc atccggcaac gatgccgact gcctgatgcg acgcttgcgc gtcttatcag
    25681 gcagcctctc atctgtataa atttcgaact acacttaact ggcttctctt aactgaggtc
    25741 accatcatgc cgttaagtcc ctacctctct tttgccggta actgttccga cgcgattgcc
    25801 tattatcaac gtacgttggg cgcggaactg ctctataaaa tcagcttcgg cgaaatgccg
    25861 aaatcagcgc aggacagcgc cgagaactgc ccttccggaa tgcaatttcc cgataccgcc
    25921 atcgctcatg ccaacgtgcg cattgccgga agcgacatca tgatgagcga tgctataccg
    25981 tcaggaaaag ccagctactc cggctttacg ctggtgctcg attcgcaaca ggtcgaagaa
    26041 ggaaaacgct ggtttgataa ccttgccgct aacggaaaaa tcgaaatggc ctggcaggaa
    26101 actttctggg cgcatggctt tggcaaagtc accgataaat ttggcgtacc gtggatgatt
    26161 aatgtcgtca aacaacaacc aacgcaataa cccgccggga ggcccgccct cccgcactgt
    26221 catcgaattc ccgttaactc ttcatctgtt agtcactttt aattaaccaa atcgtcacaa
    26281 taatccgcca cgatggagcc acttttttag ggaggctgca tcatgcaaac gattatccgt
    26341 gtcgagaagc tcgccaaaac cttcaatcag catcaagcgc tgcatgcggt tgatctgaac
    26401 attcatcaca gtgaaatggt ggctctgctt gggccgtcgg gttccggcaa atccaccctt
    26461 ttacgtcatt taagcggttt gattaccggc gataaatccg ccggtagcca tatcgagcta
    26521 ctgggccgca ctgtccagcg cgaaggccgc ctggcccgcg atatccgcaa aagccgcgcc
    26581 aacaccggct acatctttca acaattcaac ctggtgaacc gcctgagcgt actggagaac
    26641 gtgctgattg gcgcgctcgg cagcacgccg ttctggcgca cctgttttag ctggttcacc
    26701 ggcgagcaga aacagcgcgc gttacaggcg ctgacccgcg ttggcatggt gcattttgcc
    26761 catcagcgcg tttccaccct ctccggcggc cagcagcaac gtgtggcgat tgcccgtgcg
    26821 ctgatgcagc aggcgaaagt gattctggcc gatgaaccca tcgcctcgct ggacccggaa
    26881 tcagcgcgca tcgtgatgga caccctgcgc gacatcaacc agaacgacgg catcaccgtg
    26941 gtcgtcacgc tgcatcaggt ggattacgcc ctgcgctact gcgaacgcat cgtcgccctg
    27001 cgtcaggggc acgtcttcta cgacggcagc agccaactgt ttgataacga acgttttgac
    27061 catctctacc gcagcattaa ccgcatcgaa gagaacgcga aagctgcctg atatccccat
    27121 cattgaggaa aacgaatgaa cgctaagata attgcctcgc tggccttcac cagcatgttc
    27181 agcctcagca ccctgttaac cccggcacac gccgaagagc aggaaaaggc gctgaatttc
    27241 ggcattattt caacggaatc acagcaaaac ctgaaaccgc aatggacgcc gtttttacag
    27301 gatatggaga agaaactggg cgtgaaggtc aacgccttct ttgccccgga ctacgcgggc
    27361 attatccagg ggatgcgctt caataaagtg gatattgcct ggtacggcaa cctgtcggcg
    27421 atggaagcgg tggatcgcgc caatggccag gtcttcgccc agacggtcgc ggcggatgga
    27481 tcgccgggtt actggagcgt gttgatcgtc aacaaagaca gcccgatcaa caacctgaac
    27541 gatctgctgg cgaagcggaa agatctcacc ttcggcaatg gcgatcctaa ctccacctct
    27601 ggcttcctcg tccccggtta ctacgtcttc gctaaaaaca atatctccgc cagcgacttc
    27661 aagcgcaccg tcaacgccgg gcatgaaacc aacgcgctgg ccgtcgccaa caagcaagtg
    27721 gatgttgcca ccaacaacac cgaaaacctc gacaagctga aaacctccgc gccggagaag
    27781 cttaaagagc tgaaggtgat ctggaaatcg ccgctgatcc taggcgatcc gatcgtctgg
    27841 cgcaagaatc tgtccgaaac taccaaagac aagatctacg acttctttat gaactacgga
    27901 aaaacgccgg aagagaaagc ggtgctggaa cgcctgggct gggcgccgtt ccgcgcttcc
    27961 agcgacctgc aactggtgcc gattcgccag ctcgcactgt ttaaagagat acaaagcgtg
    28021 aaaggcaata aagggctgaa tgagcaggac aagctggcga aaaccaccga gattcaggcg
    28081 cagctggatg acctggaccg cctgaacaac gcgttaagcg cgatgagttc ggtgagtaaa
    28141 gcggtgcagt aaatcgtagg tcggataaga cgccccggcg tcgcatccga caatgtgtag
    28201 gcgttgatgc cggatgcggt gcaagcacct tatccggcct acagaccgga gccaaacatg
    28261 caaaccatca ccatcgcccc acccaagcgt agctggttct cgcttctgag ctgggccgtt
    28321 gttctcgacg tgctggtcgt ctcgtggcag ggcgcggaaa tggccccgct tacgctgatt
    28381 aaagacggcg gcaacatggc aacctt
//
LOCUS       DAJTUI010000049        28299 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTUI010000049 DAJTUI010000000
VERSION     DAJTUI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.049"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(30..1016)
                     /gene="araH"
                     /locus_tag="OO464_002248"
     CDS             complement(30..1016)
                     /gene="araH"
                     /locus_tag="OO464_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR6227385.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(1031..2545)
                     /gene="araG"
                     /locus_tag="OO464_002249"
     CDS             complement(1031..2545)
                     /gene="araG"
                     /locus_tag="OO464_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR6227386.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(2615..3604)
                     /gene="araF"
                     /locus_tag="OO464_002250"
                     /pseudo
     CDS             complement(2615..3604)
                     /gene="araF"
                     /locus_tag="OO464_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            4401..4904
                     /locus_tag="OO464_002251"
     CDS             4401..4904
                     /locus_tag="OO464_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6227387.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(4984..5235)
                     /locus_tag="OO464_002252"
     CDS             complement(4984..5235)
                     /locus_tag="OO464_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6227388.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(5350..5436)
                     /gene="azuC"
                     /locus_tag="OO464_002253"
     CDS             complement(5350..5436)
                     /gene="azuC"
                     /locus_tag="OO464_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6227389.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            5699..6022
                     /locus_tag="OO464_002254"
     CDS             5699..6022
                     /locus_tag="OO464_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6227390.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            6193..6690
                     /gene="ftnA"
                     /locus_tag="OO464_002255"
     CDS             6193..6690
                     /gene="ftnA"
                     /locus_tag="OO464_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6227391.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(6727..6966)
                     /locus_tag="OO464_002256"
     CDS             complement(6727..6966)
                     /locus_tag="OO464_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6227392.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            7156..8367
                     /gene="tyrP"
                     /locus_tag="OO464_002257"
     CDS             7156..8367
                     /gene="tyrP"
                     /locus_tag="OO464_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6227393.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(8429..9094)
                     /gene="yecA"
                     /locus_tag="OO464_002258"
     CDS             complement(8429..9094)
                     /gene="yecA"
                     /locus_tag="OO464_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6227394.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(9290..9376)
                     /locus_tag="OO464_002259"
     tRNA            complement(9290..9376)
                     /locus_tag="OO464_002259"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9340..9342),aa:Leu,seq:taa)
     gene            complement(9389..9462)
                     /locus_tag="OO464_002260"
     tRNA            complement(9389..9462)
                     /locus_tag="OO464_002260"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9428..9430),aa:Cys,seq:gca)
     gene            complement(9517..9592)
                     /locus_tag="OO464_002261"
     tRNA            complement(9517..9592)
                     /locus_tag="OO464_002261"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9557..9559),aa:Gly,seq:gcc)
     gene            complement(9744..10292)
                     /gene="pgsA"
                     /locus_tag="OO464_002262"
     CDS             complement(9744..10292)
                     /gene="pgsA"
                     /locus_tag="OO464_002262"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6227395.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(10349..12181)
                     /gene="uvrC"
                     /locus_tag="OO464_002263"
     CDS             complement(10349..12181)
                     /gene="uvrC"
                     /locus_tag="OO464_002263"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6227396.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(12178..12834)
                     /gene="uvrY"
                     /locus_tag="OO464_002264"
     CDS             complement(12178..12834)
                     /gene="uvrY"
                     /locus_tag="OO464_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6227397.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            13130..13306
                     /locus_tag="OO464_002265"
     CDS             13130..13306
                     /locus_tag="OO464_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227398.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            13293..13517
                     /locus_tag="OO464_002266"
     CDS             13293..13517
                     /locus_tag="OO464_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6227399.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(13550..14170)
                     /gene="sdiA"
                     /locus_tag="OO464_002267"
                     /pseudo
     CDS             complement(13550..14170)
                     /gene="sdiA"
                     /locus_tag="OO464_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(14400..15152)
                     /gene="yecC"
                     /locus_tag="OO464_002268"
     CDS             complement(14400..15152)
                     /gene="yecC"
                     /locus_tag="OO464_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6227400.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(15149..15817)
                     /gene="tcyL"
                     /locus_tag="OO464_002269"
     CDS             complement(15149..15817)
                     /gene="tcyL"
                     /locus_tag="OO464_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6227401.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(15832..16818)
                     /gene="dcyD"
                     /locus_tag="OO464_002270"
     CDS             complement(15832..16818)
                     /gene="dcyD"
                     /locus_tag="OO464_002270"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6227402.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(16923..17723)
                     /gene="tcyJ"
                     /locus_tag="OO464_002271"
     CDS             complement(16923..17723)
                     /gene="tcyJ"
                     /locus_tag="OO464_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6227403.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(17811..18362)
                     /gene="fliZ"
                     /locus_tag="OO464_002272"
     CDS             complement(17811..18362)
                     /gene="fliZ"
                     /locus_tag="OO464_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR6227404.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(18408..19127)
                     /gene="fliA"
                     /locus_tag="OO464_002273"
                     /pseudo
     CDS             complement(18408..19127)
                     /gene="fliA"
                     /locus_tag="OO464_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(19292..20944)
                     /gene="fliC"
                     /locus_tag="OO464_002274"
     CDS             complement(19292..20944)
                     /gene="fliC"
                     /locus_tag="OO464_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6227405.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            21210..22616
                     /gene="fliD"
                     /locus_tag="OO464_002275"
     CDS             21210..22616
                     /gene="fliD"
                     /locus_tag="OO464_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6227406.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            22641..23051
                     /gene="fliS"
                     /locus_tag="OO464_002276"
     CDS             22641..23051
                     /gene="fliS"
                     /locus_tag="OO464_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6227407.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            23051..23416
                     /gene="fliT"
                     /locus_tag="OO464_002277"
     CDS             23051..23416
                     /gene="fliT"
                     /locus_tag="OO464_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR6227408.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            23494..24981
                     /gene="amyA"
                     /locus_tag="OO464_002278"
     CDS             23494..24981
                     /gene="amyA"
                     /locus_tag="OO464_002278"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6227409.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(25015..25428)
                     /locus_tag="OO464_002279"
     CDS             complement(25015..25428)
                     /locus_tag="OO464_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6227410.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            25615..26820
                     /gene="yedE"
                     /locus_tag="OO464_002280"
     CDS             25615..26820
                     /gene="yedE"
                     /locus_tag="OO464_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6227411.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            26817..27050
                     /gene="yedF"
                     /locus_tag="OO464_002281"
     CDS             26817..27050
                     /gene="yedF"
                     /locus_tag="OO464_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6227412.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            27157..27827
                     /locus_tag="OO464_002282"
                     /pseudo
     CDS             27157..27827
                     /locus_tag="OO464_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(27867..>28299)
                     /locus_tag="OO464_002283"
     CDS             complement(27867..>28299)
                     /locus_tag="OO464_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6227413.1"
                     /translation="NHVMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQG
                     WYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTAN
                     ADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         7060 a         7294 c         6729 g         7216 t
ORIGIN      
        1 aaaacgttaa gttgttgtgg aaaaaagcat cagacagtgc gcttcgcttt ttgcttgtaa
       61 cggtcgaaga tcaccgctgc cagcaggatt aagccgcgaa ccacgtactg cgcgaaagga
      121 gaaatattaa gcaggttcat ggcgttttcc acggtgccta aaattaagat acccgccacc
      181 acatatgaga tttttccgat gccacctttc agagaaacgc cacctaaaac gcaggcggag
      241 ataacaataa gctcataacc aatcgacgtc attggctgcc cactagtcat acgtgaagcc
      301 agaataattc cggctatcgc agataccagg ccagagagaa caaagataat aattttggtg
      361 cgaacaaccg gtacacccgc cagacgcgca gcctcttcgt tcccgccaat cgccagggta
      421 ttacgaccaa aggtggtttt attcagcagc aaaccaaaga taatcagaca cgcgacggtg
      481 agccagattg gcgcaggcag accgaaccag ttagcgtaac caagggcaaa gaagctttca
      541 tcttcgatac cgaccgcttt accgtctgaa atgatgtacg ccagacctcg aacaatctgc
      601 atcgttgcca gtgtcgtgat cagagcattt attttcagtt tggcgataac aaagccattg
      661 accaggccac agagaacgcc cagcaacaaa cccgctgcca cgccaatcca caggctttca
      721 gtcaggttga taaccaccgc cgtggtgaca cccgcacagg caattacgga ggcgacagaa
      781 aggtcaaagt caccggaagc aaggcagaac aacatgccac acgccaccat ccccgacatg
      841 gaaattgcca ggcccaaccc tttcatatta atgaaggtgg caaaatttgg gacaaaaatg
      901 gcacaggcga taaagagcac cgcaaacacc accagcatgc cgtactgatc ccagatacgc
      961 ccgaagctga atgacgactt aggtgcgcca gaccccgatg tagaaacaga agacatcata
     1021 ctctccttac tcaggcaact gcctggctga ctttaggcat cgcaaggctc agtgcctgac
     1081 gctcatctgc ctgctcgtgt aacaattcac cggcgatttc gccttcccgc atcaccacaa
     1141 tccggtcggc aacgccgagg acttcaggta agtcgctgga ggcaaacagc accgccacgc
     1201 cctgcgccgc cagcgcataa attacgttat atatttcgtg cttagcgcca acatcaatgc
     1261 cgcgcgtagg ttcatccagc aaaatgacct tcatctcttc cgataaccag cggcccagaa
     1321 tggctttttg ctgatttccg cctgagagat tcatgatcag ttgctccgcg cccggcgttt
     1381 tgatgttgag cgaacgaatg tgctgatcgg cattgttttc ttcccaactg ttgttgatta
     1441 cacaaccgcc gagcacatgt ttgcgtctgg cactgatgtt gatattgtcg cgaacggagt
     1501 gcacgggaat aatgccttcc gctttgcgag cttccgggca gagcatcatg cctgcggcaa
     1561 tggcgtggct cggtttacga atatcgatcg gctgttggtc gatataaacc tgaccggcgg
     1621 tgatttgcgt cccgccaaac aagcctttca ttaattcgct acgccccgcc cctaccagac
     1681 caaacagccc aacaatttca ccactgcgaa ccgccagact tattggcgta cgcacgccta
     1741 gtgctttcac agcatcaaga cgcaggcgct cctcgccata actacgaggt tgccagccgt
     1801 agatatcgcc aatgtcgcgc ccgaccatcg cctgcaccag cgcgtcgtgg tcaacctgct
     1861 gcatatcggt aaaggttttg acataacgtc catctttaaa gacagtgatg gcatcgctga
     1921 gggcaaatat ttcttccata cggtgagaaa cgtataagat cacccgcccc tcttttcgca
     1981 gttcacgaat aacgcggaaa agattgccga tttcgcgggc agaaagggag ctggttggct
     2041 catcaaaggc gataatttta gcgttacgcg ccagcgcttt ggcgatttca accatctgcc
     2101 actgaccaat ggcgagatat ttcagcggcg tgtccgggtc aatatccata ccaagatgtt
     2161 taagttgtaa acccgcctca taattcagca atgagcgatt cacaatgccg cctttatgcg
     2221 gcagctggcc gagatagatg ttttccgcga cggtcatttc cggcacgaga tgcagttcct
     2281 ggtaaataat cgccacaccc gcgttaagtg ctgcggtcgt gtcggaaaag gacatttcct
     2341 gcccattaat cactacagaa cccgtggttg gcgcatagtt gccgctgagg atttttaaga
     2401 gagttgattt tcctgcgcca ttttcaccca tcaacgcatg aacctgaccg gcatagcagt
     2461 caaaactaat atccgtcagc gccttaacgc cgggaaacgt tttaccgatg ccgcgaaatg
     2521 agagatacgg ggtagactgt tgcataacgt ctccgtgaat cactggtcgt tcacaccccc
     2581 tctgcatcat gcagagggga atttttccgg caaattactt accgcctaaa ccttttttct
     2641 ccagttcttc tttaaagttg tcacgcgtga tcagtaccac gtctgtaact tcggtaaatt
     2701 ttggcgtttc aacgtctttt gctacccagt tgtaaagcat ttcgctggat ttatagccat
     2761 gtacgtccgg gcttggcagc agggaaccgt agaagccggt tgcctgtgct ttagacagtt
     2821 cgcttactgc atccacaccg ttaataccaa taccgatgat atcggctgct ttaaagccct
     2881 gaccttcggt tgcacgtacg ccgccaagta cggtgctgtc gttcataccg acgatcagcc
     2941 aatgtttaac ttccggatgt tgaaccagca ttgagttggc agcgtcaaat gcccccggga
     3001 tgtcgttaga tttggtaggt acctgataaa tttgtttttc cgggaatcct gcagctttca
     3061 gtgcgtccat agaacccgtt gtacggcggc gggcggtatc cagttcgttg gcggtaatcg
     3121 ccatcactgc gctttctttg acatcccagc cacgtttctg catctcttta tacagttcct
     3181 agccctgacg ttcgccaatt ttagtcgccg ccatcatcac cagaggaacg gtatccattg
     3241 gcttaccttt ggcgttaaca aactggtcat ccactgcaat gactttcata tcgtagccac
     3301 gcgctttcgc gacgatggca gagccgagtt tcgggtccgg agtacaaata acgaaacctt
     3361 ttgcgccact ggctgccagg ctgtcgatcg cgttcaatgt tttttcgcca tccggcacgg
     3421 caatcttaat aacctcaaac cctaaatcct tcccggcttt atcggcaaac ttccattcgg
     3481 tctggaacca cggctcttcc ggttgcttca ccagaaaacc gagcttcagg ttctccgcca
     3541 tagcggattg tgacataacg gctgccagac caatggctgc cagggcttta gtaaatttgt
     3601 gcatggttct ctccagcttt agtgtcgttt tgtgtagggc aaaaacgaat gacattcgtt
     3661 aaattaatcg gaaaacaaag cattaccttt tatctaaaag ataagtgacc gtgttgacat
     3721 agttttagcg agaaattaat tctccatagg agagcaatat cacatcgcag aattacagtg
     3781 agaacgtgca taaatttagc gggaaaagac ataaggaaaa gccaatttgt cagacaaatt
     3841 gttgaatgca cagcagatta atccataaga ttagcctgga aatccttgtt gtcttcggta
     3901 cccatgcggg atgtcttctt tttaaccagt caataggccg cattacctgg cgttgagttt
     3961 ttgaaatggt gtaataacca caactcaaag atgtggaaaa tgcgcgtcat tcatttcgcc
     4021 attaattatc actgtgctca ttaattaaca gaacacgtat aatgagagcc atctcgcaaa
     4081 aatgaaaaaa cgttttataa aatcatcact tcatcatgaa ttcaaattca tcgattaata
     4141 tcaacaagat acaaaaagca ctatcattaa aattcattgc atttacattg atttcaccaa
     4201 tgaaatgtaa aaatatataa acttgatgat ttaagcattt tcttataacc gttcagacgt
     4261 tattcttatt tcagatcatc gtcagaattg actccacgat cacatttcgg accggcagaa
     4321 aggaattatt ctgcaaacag taattatggt gttttgattt atcttgcacc tctccacttc
     4381 tggatataag gatattaggt atggcaaccg ctggaatgct tctcaaactc aactctcaaa
     4441 tgaaccgcga gttttacgca tccaatctct accttcacct gagtaactgg tgttctgaac
     4501 agagtctgaa cggcaccgcc actttccttc gcgcccaggc acagagtaat gtgacccaaa
     4561 tgatgcgcat gtttaacttt atgaagagtg tcggcgctac ccccatcgtt aaagccattg
     4621 atgttccagg tgaaaaactg aactctctgg aagaactgtt ccaaaaaacg atggaagaat
     4681 acgagcaacg ttcaagcact ctggcgcagt tagccgatga agcgaaagaa ctgaatgatg
     4741 attcaaccgt caatttcctg cgcgatctgg aaaaagaaca gcagcatgat ggcctgttgc
     4801 tgcaaaccat tcttgatgaa gtgcgcagtg cgaaacttgc gggtatgtgc cctgtgcaga
     4861 ccgaccaaca tgttctgaat gtcgtgtcac accagctgca ttgatcatac atcggcgcta
     4921 atacattgcg ccgatgaagt ttttgagaaa ccgctgcctc atctgtttga agcagcggtt
     4981 tttttaatgg gattcaccct gtggggtaaa cttgagttca atcagcgcga tggctttttg
     5041 gattgcccgc atggtgaccg ggtctgcggc agcgggatga tgagtaaagt cgatattctt
     5101 cagctgactg gacatttttt cacgaacttc aacgggcgcg attacatcga gaacatccag
     5161 aatttgtttg ataaccaact ggcaagctac cacatcagaa accagttcct gatcggcatt
     5221 cagcggctgg gacatcgtaa actcctgata gcattttgaa agccgttata gtagcggctt
     5281 cacatcttca gcgatagtca catccaccgt catcaggaca caaaaaaacc tgccggagca
     5341 ggttttttgt tatcggaaca tattgcctgg cggtacgtct ttgaacgtct tgcaatagtt
     5401 attgaacata cttttcagga ttttgcgcag tttcatcgcg gcactccgac catttgttat
     5461 acaggtgtta ttgtctttgc gcttataata tgacaaccat cacaaaaatc aatctttatg
     5521 tgatacaaat cacataaata cccctttaat gttataaaaa tgataatcaa aaaacagccc
     5581 ccctatttct gacacctaca gatggcaaga aatagcgcct gccaggcgtc ttttccggcc
     5641 attgtcgcag cgctgtaacg cgtaaaatag tgctttctct tactcttctg gctggaccat
     5701 gagacttctg attctgactc tttcattaat aacgctggcg gggtgtacgg ttactcgcca
     5761 ggcccatgtg agcgaagttg acgcggcaac aggtattgta cggttggttt atgaccaggc
     5821 ttttttgcag catgcccata ccgatcgtta tgtcagtcgc gggatcgccg atcgcgcatg
     5881 ccagcaggaa ggctataccc acgcgatccc ctttggtcag ccagtaggca actgtagcct
     5941 ttttgcgggt tccttatgtt tgaatacgga attcactttg tcatatcagt gccatcattc
     6001 cgccttccct gtctttctct aaatatttta atcagcgagg ggatcttcgc tgattaaaga
     6061 aatagcggta atgcgtttta ttcccattcg tatttttaat aattgaagtt tatattttac
     6121 cttttgcaaa taataaaata acaaattata gtgacgccac agcaacaaat ataacctttg
     6181 tggagcacta tcatgctgaa accagaaatg attgaaaaac ttaatgagca gatgaacctg
     6241 gaactgtact cttcactgct ttatcagcaa atgagcgcct ggtgcagcta tcataccttc
     6301 gaaggtgctg ccgcgttcct gcgccgtcac gcccaggaag agatgacgca tatgcagcgt
     6361 ctgtttgatt acctgactga taccggcaat ttaccgcgta ttaataccgt tgaatctccg
     6421 tttgctgaat attcctcact tgatgaatta ttccaggaaa cctataaaca cgaacaatta
     6481 atcacccaga aaattaacga actggctcat gctgcaatga ccaatcagga ctacccaaca
     6541 tttaatttcc tgcagtggta tgtttctgag cagcatgaag aagagaaact gttcaaatcg
     6601 attattgata aattaagcct ggcaggcaaa agcggcgaag gtctgtattt tatcgacaaa
     6661 gaactctcta ccctcgacac acaaaactaa tgctctccgg cggcaggttt ctgccgccgt
     6721 ttcaaatcag tgacggcaaa tcttactctg atcggtcgaa aaaccttctt ctgaaggtat
     6781 aaatttgcct tttgctgcca gaaacgctac cagctctccc gctgtcaccc cttctgccga
     6841 gcacgtgtga aaacgtgcct gttcaccaaa acgcgcttta atcgcagctt ccagactggc
     6901 atgcgtatat tgctcgcctg attcaatcat catatttaac acttcatgac cgtgaataga
     6961 gtccatcgtt cctcctcaaa aaaagcctag cgtagcgatt accgcttatg aagactttgc
     7021 gccagcgcag gactgaatgc ttttattgta catttatatt tacacaatat gtaacgtcgg
     7081 tttgacgaag cagccgttat gccttaacct gcgccgcaga tatcactcat aaagatcgtc
     7141 aggacagaag aaagcgtgaa aaacagaacc ctgggaagtg tttttatcgt ggcgggaacc
     7201 acaattggcg caggcatgct ggcaatgccg ctggctgcgg ccggtgttgg ttttagcgtt
     7261 acgttaatct tgttgattgg gctttgggcg ttgatgtgct acacggcgct attactgctg
     7321 gaggtgtacc agcatgttcc ggcagatacc ggtctgggca cgctggcaaa acgctatctg
     7381 ggacgctacg gtcaatggct gacgggcttc agtatgatgt tcttaatgta tgctctgact
     7441 gcagcataca tcagcggtgc cggtgaattg ttggcctcca gcatcagcga ctggacaggt
     7501 atttcaatgt cggcaaccgc tggcgtgctg ttgttcactt ttgttgccgg tggcgtggtt
     7561 tgtgttggaa catcgctggt cgatttattt aaccgttttc tgttcagcgc caaaattatt
     7621 tttctggtgg tgatgctggt attgctgcta ccgcatattc acaaagtgaa tcttttaacc
     7681 ctgccgttgc aacaggggct ggcactgtct gcaatcccgg tgatttttac gtcgtttggt
     7741 tttcacggta gcgtgccgag tattgtcagc tatatggatg gcaacattcg taagctacgc
     7801 tgggtgttta taatcggtag tgccatcccc ctggtggcat atattttctg gcaggtggcg
     7861 acgcttggca gcattgattc aacaaccttt atgggattgc tggctaatca tgctggatta
     7921 aacgggctgt tacaggcgtt acgcgaaatg gtggcctctc cgcatgttga gctggcggtg
     7981 catttatttg ctgatttagc cctcgccacg tcatttctcg gcgttgcgtt aggcttattt
     8041 gattatctgg ctgatttgtt tcagcgttca aataccgttg gtggacgatt gcaaactggt
     8101 gcaattacct ttctgccgcc gttggcgttt gcactgtttt atccacgagg atttgtgatg
     8161 gcgctgggtt acgccggtgt ggcgctggcg gtactggcat tgattatccc ttcactattg
     8221 acctggcaaa gcagaaagca caatcctcag gcgggttacc gggtcaaagg tggtcgtccg
     8281 gcgctggtgg tggtgtttct ctgtggtatt gctgtgattg gcgtgcaatt tttgattgcg
     8341 gcagggttgt taccagaagt ggggtgatca gatagcctca aattccttat tgggtgccag
     8401 aattaacgct gacacccaat gtgacctctt aatgcaggca gcactgctta aatttcttac
     8461 cactgccgca cggacaagga tcgttacgcc ccggtttctc ttctgctttg atcggttgct
     8521 gaacagcttt ttcctgcgga tgcgccatcc agtatgcatg taaatcaagc gccgccagtc
     8581 gaatggcatc tacgctctct tcaaacgctt ctggcgacat tttctctacc cgctcgaagt
     8641 tttcctcagt accgtgcagc gcaatcgcct ccagcgctgg ttttaaagaa tcgggcaacg
     8701 ttgaccagtc agaaagtgcc acgccccgca tatagccaaa gcaccactcc tcaacaatcg
     8761 tcagctcgct gccatcaact tctcgcaagc cgaataacgg ctcaaactgc tccgggaatt
     8821 cgttcagacg ctctgcggta tcggccatat gttgaaaagc caggttcata aagcgcgtca
     8881 tctctttctc tgacgcccaa cgcggcacat agtcagcccc accccacacg gcaaccagcc
     8941 actgttctgg ttcaatctct tgcggagaac tcaacaccgc cgtcaataaa ccgtccagct
     9001 ccgccacatc aaggatggcg tggtcagtgt tgtatttggt cagaatatcg tccagccatt
     9061 ccaactcact ttcgtttaac ggtcccgttt tcatacgctt ttccttgtgg atctcaactc
     9121 gccagcacct atcttacatg ccggtccgta tcagagatac tttttgagtg gctttgccgg
     9181 tgattaaaaa ttaaggaggg tgtaacgaca agttgcaggc acaaaaaaac cacccgaagg
     9241 tggtttcacg acactgctta ttactttgat tttattctta tctttcccat ggtacccgga
     9301 gcgggacttg aacccgcaca gcgcgaacgc cgagggattt taaatccctt gtgtctaccg
     9361 attccaccat ccgggctcgg gaagaaagtg gaggcgcgtt ccggagtcga accggactag
     9421 acggatttgc aatccgctac ataaccgctt tgttaacgcg ccaaattctt caggcctttc
     9481 agccagacat ccgcttgacg ccgatgtctt ttaaactgga gcgggaaacg agactcgaac
     9541 tcgagacctc gaccttggca aggtcgtgct ctaccaactg agctattccc gcattcatca
     9601 agcaatcagt taatcacttg attttattat cgtctggcaa tcagtgccgc cgttcgatgc
     9661 gttgcattct acttacctgg cgcgatgagt caacgatatt tttcaccact tttgatcgtt
     9721 tgctgaaaat tacgccgaaa cgatcactga tcaagcaaat ctgcacgcgc agcgctcaaa
     9781 tattgcaaca ttgaccacag agtcagtacc gcagccacaa agaaaagtgc aataccgacg
     9841 tactcaaccc aaatgttcgg acgccacagc agccaggcca acgccaccat ctgggcagtg
     9901 gttttcactt tcccaatcca ggagacggcc acgctactgc gtttacccaa ctccgccatc
     9961 cattcgcgta gcgcagaaat aataatttca cgggcgatca tcgttgccgc cggtaatgtc
    10021 acccaccagc tgtgataatg ctcggttacc agcaccatgg cgatagccac gagaacttta
    10081 tctgccacag ggtcaaggaa agcaccaaac cgggtactct ggttccagcg gcgtgccaga
    10141 aaaccatcga accagtcagt caccgccgcg acgcagaaaa tgagcgcggc ggcaaacggc
    10201 gaccaggtga caggcagata aaagaccaat acaaagaatg ggataaggat gacacggaac
    10261 agtgtaagca acgtagggat attaaattgc ataatgacgg gtaactatct gttgtcagta
    10321 agattacccc tatgttgcta cagagacatc aatgtttcaa cgaccagaag atcttttctg
    10381 ccagaccttg cgaaataccc ggcacttttg caatttcctc gacgctggcg ttacgtaaac
    10441 cttgcaaacc gcccatatat ttcaacaaca tttgccgacg ttttggcccg acgccttcaa
    10501 tggtttccag ggaactggta tttttgacct tcgcccgttt tttacggtgc ccgccaatcg
    10561 cgtgatcatg tgattcatcg cgaatatgct ggataacatg cagcgcgggt gaatctggcg
    10621 gcaaactaaa tccctcacct tccggctcaa agaacagcgt ttccagtcca gccttacgat
    10681 ctgctccttt ggcaacgcca agtagcagcg gatgattttt atcccatgag acatccagtt
    10741 cggcgaagac atttttcgcc tgcgcaagct ggcctttgcc gccgtcgata aggatcacat
    10801 ccgggatctt actgtcgtca atggctttac cataacgccg acgcagcacc tgattcatcg
    10861 ccgcataatc atcgcccggc gtgatgcctg taatgttata gcgccgatac tccgcacgca
    10921 gcgggccgtt agcatcaaac accacacagg aagcgacggt ttgttcgccc atggtatggc
    10981 tgatgtcaaa gcactccatc cgcttcactt ccggcaattt caacacgctg gcaagcgcgg
    11041 tcagtcgctg gtgaacggta gattgctgcg aaagtttgct ggttaaggcc gtcgccgcat
    11101 tggtgcgcgc gagtttcaga taacgcgccc tatcgccgcg aggtttggtt tgaacattaa
    11161 tcttgcgtcc cgccagttct gaaagggaat cggcgagcag cgttttatcg ctaagattaa
    11221 aatcgagcag gatctcaccc ggtaaggtgc gcatctggct gccttgtaaa tagaactgac
    11281 ctacgaaggt ttctaccacc tcgctcagtt ccgtaccgcc aggcactttc gggaaatagc
    11341 tgcggctgcc gagcactttg ccctgacgaa tgaacaatac gtggacacaa gccatgcccg
    11401 catcgaacgc cacaccaata acgtcaaggt cgtcgccggt attggaaaca aactgttttt
    11461 cggtgacgcg tcgcaccgcc tgaatttggt cgcgaataca tgccgcttct tcaaactcca
    11521 gattctggct ggcagtttcc atacggctaa tgagttgcgt aagcacctga tcatctttgc
    11581 cagacaaaaa caggcgcaca tactcgacct gctgagcgta ttcttcttca ctcaccagtc
    11641 cttcaacgca cggtcccaga cagcgcccta tctggtattg cagacacgga cgcgagcgat
    11701 tgcgataaac actattttcg cactggcgaa tggggaaaat cttttgcagt agcgccagtg
    11761 tttcacgtac ggcatagcca ttcgggaacg ggccgaaata ttcacctttg gcatgctttg
    11821 caccacgatg catcgccaga cgcgggtggg tatcgccact caggaagata aaaggataag
    11881 atttatcatc gcgtagcaaa acgttgtaac gcggctgata gagtttgatg tagttgtgtt
    11941 ccagcaacag cgcttcggtt tctgtatgcg taaccgttac atcaatttgc tggatctggg
    12001 cgaccagcgc ttcggttttg cgcgaagcga ggttgctgcg gaaatagctg gaaagccgtt
    12061 ttttcaggtc tttcgctttg ccgacataga taaccgtacc accagcatcg tacatgcgat
    12121 aaacgcctgg ctggctggtt acggttttta aaaacgcttt tgcgtcaaac tgatcactca
    12181 ctgacttgat aatgtctccg cattacacag accatggcga attgccaggt gagtcagctc
    12241 aacatcgcca tgaatgttta gtttactgaa catacgatag cggtagctgt tcaccgtttt
    12301 cggactgaga ttgagctgtt ctgagatctc attgaccttc tggcccttag tgatcatcag
    12361 cataatctgc aattcacgtt cagacaaact ggcaaatggg ctttctgttt tttctggttc
    12421 gatctggctt aacgccattt gttgagcgat atcagaagca atgtaacgct gccctgaata
    12481 gacagaacga atcgcactca cgacttcctg cggagccgcg cctttgctga ggtagccagc
    12541 agcacctgcc tgcatgactt tcgctggtaa agggttttct gtatggaccg taagcatgat
    12601 gattttaaca tcagctgtgg aacgcgcgat tttacgcgtc gcctcaagac cgccaatgcc
    12661 cggcatactc atgtccatta gcaccacgtc aacggcattt gtccggcacc acttaacggc
    12721 gtcttcaccg cacgatgcct ctccgacgac ttttataccc ttaatatctt ccagaatgcg
    12781 tcgtatccct gcgcgcacca gttcgtggtc atcaacaagt agaacgttga tcaaaggaat
    12841 atctccagaa atagggataa cgctactgat agttagtcat tcgtatatta gcggttttta
    12901 ttgcaacttt gaaacgttaa aaatggtggg tattcgattt ttctctcgtt tttgtcaatt
    12961 cgattgtcca cagttatggt cacgcccgtc accaacggct taccggatcg tttcacagta
    13021 agccctacga tgaaatgtga caaaaattga ctttattcag caaaaatgaa aatcagcccg
    13081 ctgaatatgt aacattaatt aacccgaaca atacgggcaa aaaacattca tgataaaaat
    13141 atttattggt cattatatta acgtatttta tagcactgcc gatatcacgc tcaaaaaaca
    13201 accactgcta tttttagcaa agcttatggt atactccgcc gccttaacat ttttcaccac
    13261 aaattttcat tgcaacatga cgaggaaaat aaatgagtac gcctgatttt tctactgccg
    13321 agaataatca agaactggca aatgaagtct cctgcctgaa agcgatgctg acgctgatgc
    13381 tgcaggcgat gggacaagct gacgcgggcc gcgtgatgtt aaagatggaa aaacagcttg
    13441 cgctgatcga agacgaaacc caggctgcag tattttccaa aacggttaag caaattaaac
    13501 aagcctaccg tcagtaatat aaaaccggct gagagcgtgc ctttcagccg gttattgaca
    13561 aaatcatcgc tatctctgct gatgtcttcc cttccgccgt ccatttcaga atttcttttt
    13621 cgcgcttgct gaaattcatc tctggcgtca tcactatttc atcatttaaa cgcatcagag
    13681 ccatcagact ttcgcgcacc agtaactgca tttttaattg cagttcatca ctaagaatgg
    13741 gtatttcgcg cgtgctgcaa cgggaaaagg acaaaaagcc cagcgcccga tttggcagca
    13801 ttaaatactg agtgacaccg cggcgtaaac catgtgcgtg cgcggcttcc cataatggct
    13861 gtgcttcgct gaataagtca tcattccaca ttaaatggcc ctgactaaag ttttcagggt
    13921 tcagcacagg atcaattgcg agaaaatttt ttgcctgata ataactaacc cacgactcag
    13981 ggtaattggt gtagaaagcc actttaggtc gcgtgaatgg taccgggtgg cgaacacata
    14041 acgaatagta atcgtactcc agctgctgag cctgaagctc tatttcatgg taggcctctt
    14101 ctgcggcctc catcctctga aaacgcaaca gcatcgtgcg acgccagctg aaaaaatccg
    14161 tatcctgcat agtaaaccgc aacgcccctg agagtgagta tcatttataa tgataattca
    14221 aactatcaca taaataagat ttatatataa tttatattat tcaggcaatg aattactttt
    14281 gcaagccatc gcattctctt atgttattaa tgagttatgc tgatttgtta agcagtttta
    14341 tcacgcttga aatggcgtcc cgccccgaca ggtgaatcgt cggggctgat tttttcttat
    14401 tattgcagca gaagcttctc gaggaactgg cgggtgcgag gctgctgggg atcggcaaat
    14461 aacgcttttg ctgccccctg ctcgactatc cgcccctggt ccataaagat cgcccggtcc
    14521 gcaacatccc gggcaaagct catttcgtgt gtcacaatca ccatcgtgcg cttttcctgc
    14581 gccagctggc ggatggtgtt caggacttcg cccaccagct ctggatccag cgccgacgtt
    14641 ggctcgtcaa acaaaatcac ctcaggacgc attgccagtg cacgcgcaat cgcaacacgc
    14701 tgctgttgac cgccagacaa acgacgtgga tagctggttt ctttaccggc cagcccaact
    14761 ttcgccagca gctcgcgagc gcgcgccgtg gcctcttctt tcggttcgcc tttgacgatc
    14821 accggccctt caataatgtt ttccagcacc gtacgatgcg gaaacaaatt aaagttctgg
    14881 aagacaaacc cgacgtgctg acgcaactgg cgaatcagcg atttttgctg acttaatgaa
    14941 cgtgcagtat caatagtgat atcgccaacg gtgatcgttc ccgcttccgg ttgttccagc
    15001 agatttatgc tacgtagcaa tgtggttttg ccggaaccac tcggaccgat aattgccacc
    15061 acttcgccag gctttacctc gaggtcgata ccgtgcagca ccgtctgacc gtggaatttt
    15121 ttcaccaggt tcttaacttc aatggcactc attttggttc tctctcctgg cgattaagtt
    15181 gattctcaaa atggttctgc aacgtcgata acacagtcgc catgatccag tagatgagcg
    15241 aagccgccag gtacatggtg aacacctcca gcgtgcgcga ggtaatcaac tgcgcctgac
    15301 ggaacagctc cggcacctgg attgtcgcgg ccagcgacgt atcttttacc agactgataa
    15361 agctgttcga cagcggcggc aacgccaccc gcgccgcctg tggcaaaatc gcgcgacgca
    15421 tggtctgcca cggcgtcata ccaatactgg cagcggcttc ccactgacct ttatcaatag
    15481 aagaaatggc ggcgcgcagc gtttcggcgg cataggcggc agtgttcagc gacagaccaa
    15541 tcattgctga cggaatggga tctaattcga taccaaactg cggcaggccg taatagatca
    15601 taaacagttg tgcgatgagc ggcgtaccac gaaaaataga gatataaaaa cgcgccagcc
    15661 agcgcacggg ccagattggc gacaggcgca tcagcgcgag aataaacccg agtagtaagc
    15721 caaaaaacat gccgccgata ctgagttgca gggtataccc agcccctttc aacaggaacg
    15781 gcaaggaatc aataaccagt tgtatacttt cttgcatgag cgttcccgga gctaaacgtg
    15841 gggatgatag gcgaacagcg caggcgcgcc gccggtatga ataaacagaa tcggcccttc
    15901 atctttgaag cgtttctgac tgataccgtc aatcagcccc gccatcgctt ttccggtata
    15961 cacaggatca agcagaatgc cttccagacg cgccagcaat ttcactgctt ccatgccttc
    16021 gtcgttcggc acgccgtagc caggtgcaaa atagtcatcc cagagtaaaa tttccgctga
    16081 tgcggtcagc tccagttctt tcgcaatcgc ctgttgtagg ttaaccactt tcggcaattg
    16141 atcggcaacg gaacgcgaca cggtcacgcc aatcagttcg ctttcaggca tcaggtgttc
    16201 cagcccaaca gccagtccgg cgtgagttcc ggcactgccc gatgcgacta ccaccgacga
    16261 aatattaacc gccccttcac actgttgcgc gatttccagc gcactctcca cataacctag
    16321 cgcgccaaga gcattagaac cgccaaccgg aatgacatac gggcgaaagc cttgtgcttc
    16381 gactcgcgtc gccagttctt ccagttgggc attgggatcg gtcagtgcgt cgcacatttc
    16441 aatctgggta ttgaacagat ccagcaacaa acgattgccg ttggttaaat agttttctgc
    16501 ggttgtgcca ataggatttt ccagcagcgc cacgcagtgc agaccgagtt tcgccgcgac
    16561 tgcggcagtc tggcgcacat ggttagactg gatcgcgccg gcagtaatca gcgtatcggc
    16621 accttcgcgc agggcatctg ccgcgagaaa ttccagctta cgtaatttat tgccgcccat
    16681 tgccatgggg gtgacctcat cccgtttgat gaaaatttcc cgtccaagat aatcagaaaa
    16741 gcgcggcaga tattcgagcg gcgttggcgc gccgataaac tccaaccgtg gaaaacgggt
    16801 taaattatgc agtggcataa cagcctccga tgtgtgttgt tgtgattttc ttattatgca
    16861 cgctgaaaac gcgtaaataa aaaaggcgct agtgaaagcg cccttttttg tcattatgct
    16921 gattatttgg tcacatcagc accaaaccat ttttcggaaa gagcttgcag agtgccgtct
    16981 ttttgcattt ccgcaattgc atcattcacc gctttcagca ggtcttcatt tcctttacgc
    17041 agcgccacgc cagactcctg acgggagaat gcttcaccgg ttactgccag cgtatcgttg
    17101 gttttcttca ccagatccag cgccgccaga cgatcaacga ggatcgcatc gatacgccct
    17161 acgcgcagat cctgatattt ggtcgggtca tcatcatagg tacgcacatc gacaccctga
    17221 acattctgcc gcagccactc ttcatagttg gtgcccagac cgacacccac ttttttgcct
    17281 ttcagatcag cagccgtttt aatggtgcct tcgttacctt ttttcaccag cgcctgaata
    17341 ccggaaatgg tgtaaggggt agagaaatcg tattttttct tgcgctcgtc agaaatggtg
    17401 acctgattaa tcaccacatc aatgcgttta gagtccagcg acgccagcat accgtcccat
    17461 ttggtcggtt ttagtgacgc ctcaacgcca agatgttttg ccagctgttg ggcaaattcc
    17521 acttcaaaac cggttaattt gccgtcatcc ccctgaaaac tgaacggcgg ataagtgcct
    17581 tccagcccta ccagcagcgt gccgctctct ttgactttat taagcagacc ttcatctgca
    17641 aaacttttaa cgctcatgcc cgcaaccagc gccacggcca tcacacccat caatgcctga
    17701 cgtcccagat gtgctaattt catattcacc ccgaatgttg ttatgtctgt ttgcagtgta
    17761 gagccattgt ttgtaaacac aaaaacaact ccgctacatc ttattcttat ttaatatata
    17821 tcagaagcag gcaggctgga ggatttctgc acaagtccgg tacaggtttg gcgctgatag
    17881 tgttgatact tccgtaatgc gatgcggtaa ttgttggtgc tggtggtggg cagccacggg
    17941 gcgaggattt catcaaggaa accgtcctgc agctgatcga gggaaatatt ttgctcatgt
    18001 agatgattac ccaaacggcg cagacgaacg acatattcac gcaccgtacc tgggctcatt
    18061 tcagtttgct caaaaagaaa tttcttaaag ccgataatat cgaaaaaatc actctgcgtt
    18121 ttgcaatgta aatcaccgca aaatcggcac aatgccgccc atgattgttg ttccgtttgc
    18181 cagccttttt catcaagcag cgtgtccagg cgggaaatct cgattttatt cactattttg
    18241 ccgtcgcgaa ctaaggtaat gcgatcgagt aatttacggc aatgcgcgca atgggtctgg
    18301 ctgtgtttaa agtctttaag atagcggctt aatggccgtc ttttcaggtg ctgcaccatc
    18361 attaagaact cctggtagtc aaagttaaag tgcggcattt actgacgtta taacttaccc
    18421 agtttagtgc gtaaccgttt aatgtcctgg ctgtgtaact gactgacccg cgattccccg
    18481 acctccagca ccgcgccaat ctctttgaga ttcagctctt cctggtaata gagggttaat
    18541 accagttttt cgcgctccgg caacgtttcg atggcttcca tcacccgctg gcgcagatta
    18601 ctgtccagca gttgttgtag cgggttttct cgctgatgat catcagtaac cagttcgatg
    18661 ctatcgccgt gctcttcacg ccactcatcg taggagaaga gctggctgtt attggtgtcg
    18721 agcagcattt gacgataatc ggcaatatcg atccctaaac gttccgctac ctcagtttcc
    18781 gtggcgttgc ggccaagttc ctgctccagt tgccctattg cctgtgccac ttcacgcgcg
    18841 ttgcgtcgca cgctgcgcgg cacccagtca cggctgcgaa gttcatccag catagcgcca
    18901 cggatacgct acactgcgta agttgtaaat gccgttcctt gtagggcgtc atagcgttcg
    18961 acagcattaa gtaacccaat gccgcccgcc tgtagcagat cgtcaagttc cacgctcgcg
    19021 ggcagtcgaa cctgcaggcg caatgcttcg tgacgcacca gcgggacata acgctgccac
    19081 agcgagtgtt tatccattac accttcagcg gtatagagtg aattcacgat aaacagccct
    19141 gcgttatatg agttatcggc atgattatcc gtttctgcag ggtttttaat cggacgatta
    19201 gtgggtgaaa tgaggggtta tttgggggtt acgcaaaaag ccgaatgtaa aaaaccccgc
    19261 cggtggcggg gtttgagagg cattcagacg attaaccctg cagcagagac agaacctgct
    19321 gcggtacctg gttggctttt gccagcacgg agttaccggc ctgctggata atctgcgctt
    19381 tcgacatgtt ggacacttcg gtcgcatagt cggcgtcctg aatacgggac tgcgcttcag
    19441 acaggttggt agtggtgttg ttcaggttgg tgactgcgga atccaaacgg ttttgcaccg
    19501 cacccaggga ggagcggaat ttgtcgatgg agctgatggc ttcatccaga gctttcaggg
    19561 gatcggctgt cgtagctgct tttgtttcag catctgtcgt caatttacca tcagcatctt
    19621 tataaactgc cttaccgctg ccgttagtca ctgaaccatc atcctgtagg taaaaatcta
    19681 cagtatcaga tgtggtcaac gcctcgttgt tatcaacata aaccgaatca acaccttttg
    19741 ttacagcgcc agttgtgccg ttgacgctat aacctgcagt agtgaaatca acattgtttc
    19801 ccgaggttga tacagcttta tcgaaagctg cctgatcaac ttttgcacct gttactgaat
    19861 aagtaatagt gtccggcgta gtactattac cggtaaagga gatagaggta ccgccaatgt
    19921 caatggttgt cgctttggta ttattggctg caagtgtgga cagactagcc tcagtcaaag
    19981 aagcaccaga gccgtttttc gttaagcgcc cagttgtatc aatgtagagt ttatctccat
    20041 tgcttgccgt aattttacca tcactgctga tctgaacatc ctgtgcagaa ccatcgatag
    20101 taatagtgcc cttagcggta tcaccaactc ccggggtcaa atatttctga acatcggcag
    20161 ctgaagccgt tgtggtatca aaagagtatg acttacttgc actgtcatac ttgtaattcg
    20221 ttgcactagc cgccgttccg aagccgttat taatggttgc ctgaacagta tcaccatcac
    20281 taactccagc cagcaaatca gacgctttag cagcatcgta acccgcactc acagtatatt
    20341 tgttgccgac cactgttgca ttagcagcta ccaagtcagc tttagatgca gcagtattag
    20401 ccacagcccc gccaccattc acattaaacc cattcagccc cagcgtatca gagtcaattt
    20461 tcttcagatc aatagtgata gtctggccgt cattcgcacc aacctgaatt ttcatcgaac
    20521 cgtcttttgc cagtacgttc acgccgttga actgggtctg accggatacg cggtcaattt
    20581 cgtcgagacg ggatttgatt tcgtcctgaa tggagtccag atccgaatca gagttagtcc
    20641 cggtagaagc ctgaaccgtc agctcacgga tacgctgtaa gttgttgttg atttcggaca
    20701 gcgcgccttc agtggtctgt gcaacagaaa taccgtcgtt ggcgttacgt gcagcctgag
    20761 tcaggccttt aatgttagaa gtaaaacggt tagcaatcgc ctgacccgcg gcgtcatcct
    20821 tcgcgctgtt aatacgcaag ccagaagaca gacgctcgat agaactcgac agcgcagact
    20881 ggttcttgtt gatattattt tgagtgatca gcgagaggct gttggtatta atgacttgtg
    20941 ccatgattcg ttatcctata ttgcaagtcg ttgattacgt tttgggtttc cacccgtcgg
    21001 ctcaatcgcc gtcaaccctg ttatcgtctg tcgtaaaaca acctttagaa tttttttcac
    21061 aaacagccat tttttgttag tcgacgaaat actcttttct ctgcccctta ttcccgctat
    21121 taaaaaaaac aattaaacgt aaactttgcg caattcaggc cgataacccc ggtattcgtt
    21181 ttacgtgtcg aaagataaaa ggaaatcgca tggcaagtat ttcatcgctg ggagtcgggt
    21241 caggtctgga tttaagttca atccttgata gcctcaccgc cgcgcaaaaa gcgacgctaa
    21301 cccctatttc aaatcagcaa tcgtcgttta ccgctaaact tagcgcctac ggtacgctga
    21361 aaagcgcgct gacgactttc cagaccgcca atactgcatt gtctaaagcc gatctttttt
    21421 ccgccaccag caccaccagc agcaccaccg cgttcagtgc caccaccgcg ggtaacgcca
    21481 tcgccgggaa atacaccatc agcgtcaccc atctggcgca ggcgcaaacc ctgaccacgc
    21541 gcaccaccag agatgatacg aaaacggcga tcgccaccag cgacagcaaa ctcaccattc
    21601 aacaaggcga cgacaaagat ccgattacca ttgatatcag cgcggctaac tcatcgttaa
    21661 gcgggatccg tgatgccatc aacaacgcaa aagcgggcgt aagtgcgagc atcattaacg
    21721 tgggtaacgg tgaatatcgt ctgtcagtca catcaaatga caccggcctt gataatgcga
    21781 tgacactctc ggtcagcggt gatgatgcgc tacaaagttt tatgggctat gacgccagtg
    21841 ccagcagcaa cggtatggag gtctcggtcg ccgcccagaa tgcgcagctg acggtcaaca
    21901 acgtcgccat cgagaacagc agcaacacta tcagcgacgc gctggaaaac atcactctga
    21961 acctgaacga tgtcaccacg ggcaaccaga cgctaaccat cactcaggac acctccaaag
    22021 tgcaaacggc gattaaagac tgggtgaatg cctataactc gctaatagat accttcagca
    22081 gcctgaccaa atacaccgcc gtagatgcgg gagctgatag ccagagttct agcaatggcg
    22141 cactgctcgg cgactccacg ctgcggacga ttcagacgca gttgaagtcg atgctgagta
    22201 ataccgtcag ttcttccagc tataaaacgc tggcgcagat tggtatcacg accgatccca
    22261 gcgatggcaa actggaactg gatgccgaca aactcaccgc tgcactgaaa aaagatgcca
    22321 gcggcgtagg tgcattgatt gttggcgatg gtaaaaaaac cggcatcaca accaccatcg
    22381 gcagcaacct gaccagttgg ctttcgacaa cgggcattat taaagccgct accgatggcg
    22441 ttagtaaaac cctgaataag ttaactaaag actacaacgc cgccagcgat cgcattgatg
    22501 cgcaggtcgc gcgctacaaa gaacaattta cccaactgga cgttttaatg acctcgttaa
    22561 acagcaccag cagctactta acgcagcagt tcgaaaacaa cagtaattcc aagtaatcat
    22621 tattcatcgg gagacaggtc atgtacgcgg caaaaggcac ccaggcctat gcacaaattg
    22681 gcgtcgaaag caccgtaatg agcgccagcc aacagcagtt ggtcaccatg ctatttgatg
    22741 gagtgctgag cgcactggtt agagcgcgac tgtttatgca ggacaacaat cagcaaggca
    22801 aaggcgtctc tttgtcaaaa gcgatcaaca tcattgagaa cggactgcgg gtgagtcttg
    22861 atgaagagag caaagacgaa ttaacccaaa acttaattgc tctttatagc tatatggtca
    22921 ggcgtttgct gcaagccaat ttacgcaacg atgtctcagc agtcgaagag gtggaagcat
    22981 taatgcgtaa tattgccgat gcctggaaag agtctttact ctccccttct tcgattcagg
    23041 acccagtctg atgaacaatg caccgcattt gtatttcgcc tggcaacaac tcgtcgaaaa
    23101 aagccagctc atgttacgcc tggcaacgga agaacaatgg gacgaactca tcgctagcga
    23161 aatggcgtat gtgaatgcgg tgcaggagat cgcacacctg actgaagagg ttgccccgtc
    23221 cactacgatg caggagcagc tccgcccgat gctgcacctg attctcgaca acgaaagcaa
    23281 ggtaaagcag ttattacaga ttcggatgga tgaactggcg aaactggtcg gtcagtcatc
    23341 ggtgcaaaaa tcggtgttaa gtgcctatgg cgatcagggc ggctttgtgc tggctccgca
    23401 ggataacctc ttttgaatct taatgagccg atggctcgcg aataatccga ttacggctac
    23461 gcttctaata ttccccttga atggagtcga agaatgcgta atcccacgct gttacaatgt
    23521 tttcactggt attacccgga aggcggtaag ctctggcctg aactggccga gcgcgcagac
    23581 ggttttaatg atattggtat caatatggtc tggttgccgc ccgcctataa aggcgcatcg
    23641 ggcggctatt cggtcggcta cgactcctat gatttatttg atctaggtga gtttgatcag
    23701 aaaggcagca tccctactaa atatggtgat aaagcacaac tgctggccgc cattgacgcc
    23761 ctgaaacgta atgacattgc ggtgctgctg gacgtggtag tcaaccacaa aatgggcgcg
    23821 gatgaaaaag aagctattcg cgtgcagcgt gtaaatgctg atgaccgtac gcaaattgac
    23881 gaagaaatca ttgagtgtga aggctggacg cgttacacct tccccgctcg tgccgggcaa
    23941 tactcgcagt ttatctggga tttcaaatgt tttagcggta tcgaccatat cgaacaccct
    24001 gatgaagatg gcatttttaa aattgttaac gactataccg gagaaggctg gaacgatcag
    24061 gttgatgatg aactcggtaa tttcgattat ctgatgggtg agaatatcga ttttcgcaat
    24121 catgccgtga cggaagagat taaatactgg gcgcgctggg tgatggaaca aacgcaatgc
    24181 gacggctttc gtcttgatgc ggtcaaacat attccggcat ggttttataa agagtggatc
    24241 gaacacgtac aggaagttgc gccaaagccg ctgtttattg tggctgaatg ctggtcgcat
    24301 gaagtcgata aactgcaaac gtatattgat caggtggaag gcaaaaccat gctgtttgat
    24361 gcgccgctgc agatgaaatt ccatgaagca tcgcgcatgg gtcgcgacta cgacatgacg
    24421 cagatcttca ctgggacgct ggtggaagcc gatccttttc acgccgtaac cctcgttgcc
    24481 aaccacgaca cccaaccgtt gcaagccctc gaagcaccgg tcgaaccgtg gtttaaaccg
    24541 ctggcgtatg ccttaatttt gttgcgggaa aatggcgttc cttcggtgtt ctatccggac
    24601 ctctacggtg cacattacga agatgtcggt ggtgacgggc aaacctatcc gatagatatg
    24661 ccaataatcg aacagcttga tgagttaatt ctcgcccgtc agcgtttcgc ccacggtgta
    24721 cagacattat ttttcgacca tccgaactgc attgccttta gccgcagtgg caccgacgaa
    24781 tatcccggct gcgtggtggt catgtcgaac ggagatgatg gcgaaaaaac cattcatctg
    24841 ggagagaatt acggcaataa aacctggcgt gattttctgg ggaaccggca agagagcgta
    24901 gtgacagacg aaaacggcga agcgaccttc ttttgcaacg gcggcagcgt cagcgtgtgg
    24961 gttatcgaag aggtgattta aatccatccc cggcggcaag ccggggagat ttcattacgg
    25021 cagtggcgtc ggtaacggcg ctttatccag cgcggcggca cactctgccg tcgggcgatc
    25081 cacacgctcc atcgtcatac catcgtactc aatggtgtta ccgtcacgct caacctcgta
    25141 cagctcacgt tttacggtaa cgttagtgag atcatcagac atcaatgtca acttacccgg
    25201 cacggcaatc ccccgctgcc actgacggca atccaaggta tcgccctctt tagtgacaat
    25261 caggctgcct atcgcttccg gactgaccag cgcacgttgc ggccctttgg tctgccagta
    25321 gcccgccagc cagtccggcg cgggggtttt cacgacattg ttatagtttt cgacttcggc
    25381 acagcccgcc agcaacatga gcgcgcctgc aattgcgagt tttttcatca ttcgtcctgc
    25441 atgagaagaa aagaggattg tggcattaaa gctctgatgt cgccagcctt tccaggttca
    25501 gagagaaatt tgatctaact attatttttg tgaattatta ccccgaaaat aaatttgcat
    25561 ggtgtatgat tcgcggctct tgtttcaaac cttctgagtt cagaggctac attcatgtca
    25621 tggcagcaat tcaaacacgc ctggttgatt aaattctggg cacccatccc tgcggtcatc
    25681 gcggcgggta ttctctccac ttactatttt ggcattactg gcaccttttg ggctgtcacg
    25741 ggtgaattta cccgttgggg cggtcagctc ctgcagctgt ttggcgtcca tgctgaagag
    25801 tggggttatt ttaaaattat ccatctggaa ggatcgccat taacccggat cgacggcatg
    25861 atgatcctcg gtatgtttgg cggctgcttt gctgctgcgc tgtgggccaa caatgtcaaa
    25921 ctacgcatgc cacgcagccg tatccgcatt atgcaggcca tcattggcgg cattatcgcc
    25981 ggttttggcg cgcgtctggc aatgggctgt aacctggcgg cgttctttac cggcattcct
    26041 cagttctcgc tgcatgcctg gttctttgcc atcgccactg ccattggttc atggtttggc
    26101 gcgcgcttta ccctgctgcc catcttccgt attcccgtga aaatgcagaa agtttctgcc
    26161 gcctcaccgc tgacgcaaaa accggatcag gcgcggcgtc gttttcgtct cgggatgctg
    26221 gtctttttcg gcatgctggg ctgggcgctg ctcacggcga tgaaccaacc aaaactgggg
    26281 ctggcaatgc tgtttggcgt cggctttggt ttactgattg aacgtgcgca aatctgcttt
    26341 acttcggcgt tccgcgatat gtggatcacc ggacgtaccc atatggcgaa agcaatcatt
    26401 atcggtatgg cggtgagtgc catcgggatc ttcagttacg tacagttagg cgttgaacct
    26461 aaaatcatgt gggcgggacc aaatgcggta attggtggtt tactttttgg ttttggcatc
    26521 gtgctggcag gcggctgcga aaccggctgg atgtaccgcg cggtagaagg ccaggtgcac
    26581 tactggtggg tcggtctggg caacgtgatc ggctcaacga ttctggcgta ttactgggat
    26641 gatttcgctc cggcgctggc caccgactgg gacaaaatca acctgctgaa aacttttggt
    26701 ccgatgggag gcctgctggt gacatatttg ctgctgtttg ctgcgctgat gttgattatc
    26761 ggctgggaaa aacgcttctt ccgccgtgag gcaccgcaga ctgctaagga gatcgcatga
    26821 aaaatatcgt tcctgattac cgccttgata tggtgggtga accctgccct tatccggcag
    26881 tcgcaaccct tgaggcgatg ccgcaattaa aaaaaggtga gatcctggaa gtggtgagcg
    26941 actgtccgca gtcgatcaat aatattccac tggatgcgcg taatcacggc tacacggtgc
    27001 tggatattca gcaagacggg ccgaccattc gttatttaat tcagaagtaa tccctcattc
    27061 ccgctgtggc ccacagcggg aacatcgtta cctctgacac atagatttta cccgccccct
    27121 ctcctattct tttcagtatg atctcaggag gttgctatgt gtggacgctt tgcccaatcc
    27181 caaacgcgtg aggattacct tgcgcttctc gcggaagata ttgaacgcga tattccctac
    27241 gatcccgaac ccattggcag atacaatgtc gcgccgggaa ccaaagttct tctgctcagt
    27301 gaacgtgatg aacaccttca tctggatccg gttttctggg gatatgctcc cggatggtgg
    27361 gataaaccgc cgctgattaa cgcccgcgta gaaactgcct caaccagtcg catgtttaaa
    27421 ccgctctggc aacatggtcg ggcaatctgt tttgccgatg gctggtttga gtggaaaaaa
    27481 gaaggcgaca aaaaaagcct tattttatct atcgcgctga cggacaacct gtttttatgg
    27541 ccgcgatagg tagcataccg tttgagcgtg gtgacgaagc cgaaggattt ttgatcgtca
    27601 ctgcggcggc agatcaaggt ctggtagata ttcatgaccg ccgcccgctg gttctgtcgc
    27661 cagaagctgc acgggaatgg atgcggcaag acattggtgg taaagaagcc tcagaaatcg
    27721 ctaccagaag ctgtgtgcca gcaaaccagt tcacctggca ccctgtgtcg cgcacggtgg
    27781 gtaatgtcaa aaaccagggc gcggagttaa ttcaacctgt ttgttgacat cctccacgcc
    27841 ctgaatgacg aggacccctg ctacgttcag gctgtcgcct gaatcatttc ggtgggttcc
    27901 tgcttcaacg agcggcctga ctgcaccatc tctccacagg caagaacggc gtgccccgcc
    27961 gctaaaatgt tacgagctcc atttacatcg gcgttcgctg tatatccaca tacctggcat
    28021 ctgaatttac tttgtgacag gcgattttct ttcgctgtat gaccacagca cgcgcaacgc
    28081 tggcttgtgt acgctggcgg aacagcaagc acctgaccgc cacgccagag ctgcttgtac
    28141 tcaagctggc ggcgcatttc ataccagccc tgatccagta tcgaacggtt taaacctgat
    28201 tttgcccgga cattgcgccc cggctggctg accgtacccg ctgctgactt tgacatgttg
    28261 ctgaccttca aatcctcaat gactatcatt acgtggttt
//
LOCUS       DAJTUI010000050        28225 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTUI010000050 DAJTUI010000000
VERSION     DAJTUI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.050"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(28..897)
                     /gene="yjfF"
                     /locus_tag="OO464_002284"
                     /pseudo
     CDS             complement(28..897)
                     /gene="yjfF"
                     /locus_tag="OO464_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(884..1906)
                     /locus_tag="OO464_002285"
     CDS             complement(884..1906)
                     /locus_tag="OO464_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6227414.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(1920..3422)
                     /locus_tag="OO464_002286"
     CDS             complement(1920..3422)
                     /locus_tag="OO464_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6227415.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3562..4518)
                     /gene="ytfQ"
                     /locus_tag="OO464_002287"
                     /pseudo
     CDS             complement(3562..4518)
                     /gene="ytfQ"
                     /locus_tag="OO464_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4828..5358
                     /gene="ppa"
                     /locus_tag="OO464_002288"
     CDS             4828..5358
                     /gene="ppa"
                     /locus_tag="OO464_002288"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6227416.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5738..6079)
                     /locus_tag="OO464_002289"
     CDS             complement(5738..6079)
                     /locus_tag="OO464_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6227417.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6082..9861)
                     /gene="tamB"
                     /locus_tag="OO464_002290"
     CDS             complement(6082..9861)
                     /gene="tamB"
                     /locus_tag="OO464_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6227418.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9858..11591)
                     /gene="tamA"
                     /locus_tag="OO464_002291"
     CDS             complement(9858..11591)
                     /gene="tamA"
                     /locus_tag="OO464_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6227419.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11797..12435
                     /gene="msrA"
                     /locus_tag="OO464_002292"
     CDS             11797..12435
                     /gene="msrA"
                     /locus_tag="OO464_002292"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6227420.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12758..14101
                     /locus_tag="OO464_002293"
     CDS             12758..14101
                     /locus_tag="OO464_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6227421.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14163..14369)
                     /locus_tag="OO464_002294"
     CDS             complement(14163..14369)
                     /locus_tag="OO464_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6227422.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14694..15248
                     /locus_tag="OO464_002295"
     CDS             14694..15248
                     /locus_tag="OO464_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6227423.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15311..16249)
                     /locus_tag="OO464_002296"
     CDS             complement(15311..16249)
                     /locus_tag="OO464_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227424.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16461..17201)
                     /gene="cysQ"
                     /locus_tag="OO464_002297"
     CDS             complement(16461..17201)
                     /gene="cysQ"
                     /locus_tag="OO464_002297"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6227425.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17391..19334
                     /gene="cpdB"
                     /locus_tag="OO464_002298"
     CDS             17391..19334
                     /gene="cpdB"
                     /locus_tag="OO464_002298"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6227426.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19460..19840)
                     /locus_tag="OO464_002299"
     CDS             complement(19460..19840)
                     /locus_tag="OO464_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6227427.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            19929..20789
                     /gene="qorB"
                     /locus_tag="OO464_002300"
     CDS             19929..20789
                     /gene="qorB"
                     /locus_tag="OO464_002300"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6227428.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            20898..21863
                     /locus_tag="OO464_002301"
     CDS             20898..21863
                     /locus_tag="OO464_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6227429.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            21971..22633
                     /gene="ytfE"
                     /locus_tag="OO464_002302"
     CDS             21971..22633
                     /gene="ytfE"
                     /locus_tag="OO464_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6227430.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22678..24090)
                     /gene="cycA"
                     /locus_tag="OO464_002303"
                     /gene_synonym="dagA"
     CDS             complement(22678..24090)
                     /gene="cycA"
                     /locus_tag="OO464_002303"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6227431.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24398..25018)
                     /gene="fklB"
                     /locus_tag="OO464_002304"
     CDS             complement(24398..25018)
                     /gene="fklB"
                     /locus_tag="OO464_002304"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6227432.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25237..25782
                     /locus_tag="OO464_002305"
     CDS             25237..25782
                     /locus_tag="OO464_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR6227433.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25766..26413)
                     /locus_tag="OO464_002306"
     CDS             complement(25766..26413)
                     /locus_tag="OO464_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6227434.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26625..27416
                     /locus_tag="OO464_002307"
     CDS             26625..27416
                     /locus_tag="OO464_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR6227435.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27426..>28225
                     /locus_tag="OO464_002308"
     CDS             27426..>28225
                     /locus_tag="OO464_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR6227436.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6920 a         7511 c         6969 g         6825 t
ORIGIN      
        1 aataccgatg gcgattttcg tccactagga actcagcgtg ccgtcaaagt tgatgtaagt
       61 ctggatcagt ccctgaattg ccacgccaaa aagcgtccca agcaccgtcc cgacgccacc
      121 acttaacaac gtaccgccaa tcaccaccga cgcgatggcg tccagttcca cacctacgcc
      181 cgccagtgca tatccggctt gggtataaat cgagaagaca atccccgcca gtgttgccag
      241 tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtagaaatcc ccatcaggtt
      301 cgccgacgtc gcgttgccgc caatagcgta cacctgattg ccaaaacggg tacaatgcgc
      361 aaggaaaata ccgataacca ccaccgccag catcaacagc cccatcgcac taaggcgacc
      421 gccgccgggg attttccacg caaggcttga gagcgtgtca taaatgggat ggtttatcgg
      481 aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc ccgcaagcgt
      541 aatgataaat gccgggatct tcagggcgtc gatcagaagc cccataaatg cgccgaaggc
      601 acagcccatc accagcacta gcggaaacgc cagcagcggc gagaggccga aatcgccaac
      661 cacttttgcc agaaacacgc cagtaaaggc gatcaccgaa ccgacggaga gatcgatacc
      721 gccagagagg atcacaaagg tcatgccaac ggcgattatc ccaagaaagg cgttatcggt
      781 caggatattg cagatcaccc ttgtggaagc aaaaccggga aactgggtca ggcagtagag
      841 atagcccagc acaaagacgc cgatggtgat catcagcggc aagttacgtt ttatcatggc
      901 tacgtactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc acacaaagca
      961 ccaccaccgc tttcaccacc tggttcatct ccggcggaaa gcccgaaagc aaaattccgg
     1021 tgttcatccc ctgaataatc agcgccccca ctaccgagag cagcaggtta aagcgcccgc
     1081 ccatcagcga tccgccgcca atcaccaccg cgagaatggc gtccagctcc agccataatc
     1141 cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc gcaatcgccg
     1201 cacatagccc gctcaacacg taggtgagca tgacgatgat tcgcgtgttt accccggcat
     1261 tttttgccgc ccgaatgttg ataccaacga cttcgataaa catccccagc gccgttttgc
     1321 gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc gttggcagga
     1381 acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg acgatctgcc
     1441 cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg gtggcgacaa
     1501 acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgcc aggatgccgg
     1561 tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc atcgcagccg
     1621 ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca ccggtggcga
     1681 tcaccagcgt aataccaatc gccagtaacg caacgggagc tgcacggtta agaatgtcga
     1741 tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc ggggctacca
     1801 ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc gtcggccagt
     1861 gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca ctgttctcct
     1921 tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg gatctccgcc
     1981 acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag ctcttccagt
     2041 tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat caggcggatg
     2101 atctcggcgt gggcaccaac atcaatgcca cgggttagct catcaaggat cagaaattgc
     2161 ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc ggagagaaat
     2221 tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa gcgttcggca
     2281 atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc ctggagagcg
     2341 aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt cctgtcttcc
     2401 gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt ttgcggtttg
     2461 cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac ttcggcggtt
     2521 tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg gcgcacttcg
     2581 agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc aacgggtttg
     2641 tcgctcaaca atgttcgccc ggcacgctgt agcgcgtggg tatccagctc gcgccccagc
     2701 atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc tacgaaactg
     2761 ccgttgcgta agacggtgat ccgatcgctg acctgataga cctgatcgag aaagtgagtg
     2821 acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa taatagctcc
     2881 atttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt ggcggagaga
     2941 tcgatagccc ggcaaatggc gacgatttgc tgcatcgcca ccgaaaaacg gttaagcggt
     3001 tcgcgcacgt cgagggagaa accgtaagat gccatcagtt cggtggcgcg cttttccatc
     3061 tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag attatcagca
     3121 accgacatat tgggtagcag gttgacttcc tgatagacgg tgccgatacc gagttgttgt
     3181 gcatgggcgg tatttttcgg tgagatagtc tggccttcca gccagatggt gccgcgatcg
     3241 gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc gttttcacca
     3301 agcagcgcca tgatttcgcc acggcggagg ctgaaatcaa cgttgtctaa cgctttgacg
     3361 ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg gtcggtcgtc
     3421 atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg gtaagcgtcg
     3481 catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg gcctacgaat
     3541 cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta actcttcttt
     3601 tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag gcatggtgcc
     3661 gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg gcgtcagttc
     3721 aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta caccgtcaat
     3781 ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg cctgaattgc
     3841 accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc cgttgttttc
     3901 cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt cacctgactg
     3961 cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc ctttcttacg
     4021 gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc atggtttgcc
     4081 attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt tgtcggcagt
     4141 aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga gcaagaagac
     4201 cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg caaccactgg
     4261 agcgataaag atcgcatcca ccccttatgc aacgaaggaa cgtaccgctt taatctggtt
     4321 ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt cggcctcact
     4381 tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct gcgaaaatcc
     4441 tacggttaat ggagcggcca acgccataga cgacatggct gccgagactg cagagactac
     4501 aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg tgaaaagatg
     4561 ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc acacttcaaa
     4621 aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta tgaaaaatgc
     4681 ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga aaaaggaaaa
     4741 tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactt ggcacttgtt
     4801 tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc gggtaaagat
     4861 ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc gatcaaatac
     4921 gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac cgcgatgttc
     4981 tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga cccggttgac
     5041 gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg ccgtccggtt
     5101 gccgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc ggttccgcac
     5161 agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc tgaactgctg
     5221 aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg caagtgggtg
     5281 aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc ttccttcgag
     5341 cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg cttcggttag
     5401 taagggtttt tttatgcccg cgataaataa actctctatt ccaccatcat tattctcagc
     5461 ggttgcaagg cttgaacggt aagaacaagc aaacccgacc accattttgc tgttcatagc
     5521 cacttgctgg aagttagccg acctcactca tactcaccgt tcaaagcgtg cccccgtttt
     5581 acccctgcac atttgtgaac gccagcagag ttttttatgc gattgcagat atggaaagcg
     5641 gctcggagag aaaagcaaaa gatggaaaag ttacaaaaaa aaatcgcgag atacatcgca
     5701 taaaacaacg ccacccgaag gaggcgtgct tttattatta cttatccctg tctaaccagt
     5761 cgccgctttc aattagcttt aatccatcga cgggtcgttg ataaacgtac atccatgcac
     5821 tcccatacgg cgtctgaatc aactggcgcg cgtattcacc gcccctggtg cgcaaggcat
     5881 caagttcggc cagcgtggcg ttgtcaatac gataaacttc accgtgtacc gttccgttcc
     5941 ccggaactgc gcctggatag tggcccaggc tatacaactg gtagttatcg atactgaaat
     6001 cgcccagtaa ctgggcattg gtcatccagt gactgttgcc ttgtttgtgg cgtaaactgc
     6061 cgtagacaaa tattcgcatt gctaaaactc gaactgatag agcaaatcca gtgcctggtc
     6121 tacaccagac acggcttcca gatatagctt aggcatcagg cgataacgta acgtgagtgt
     6181 tgctatagag tcaaatatac ccacgccgta tttcacttgc agacctggca atacatagcc
     6241 gctgaccact acctgggagg agtcgcctac tcccagggtg tcgagcgcta aattgcttac
     6301 gccaaacgtc tcgccgattt tacccacaat ctggccactt tgcgcaaccc ccagaccaat
     6361 cagcatcgag gtcattgccg cactgtcgct ctgatcgctc tccagccctt gtccacgtag
     6421 caaataagac aatgcagctt gttgcgacat cgccgggtca gagaagatct ccgctttcgg
     6481 ttcgtccgcc agaccagtga cgcgaactcc ggcgattaca tcgtcttctg tagcatccgg
     6541 gttacgaata gcttcaatat taagatacgg ttgatccggc ggaccagaga acagtaactc
     6601 acccttacgc acaatcagat cctgaccata ggcatggaag cgcccttcag ggatgttgat
     6661 ctgcccgttc aggcccagcc cttgtttgtc ctgtacgacg ttaagatcgc ccgtcagccg
     6721 cgctttcagg ccaaaggcgt caatgcgcac attgttgcca acgtggacaa tcaggttact
     6781 gttaatcgga atcgacgccg ttttcggctc ttccggttgc aggttatcgt taagcatcac
     6841 cacatcgctg gagacgccta ctgcgctttc cggcagatcg tgcaccacga tgcgcgccca
     6901 cgggacatcc acgcgaccat cgagggtaaa caggtttggt gtagcctcga atacaacatc
     6961 tggcgataca tccattcgta ccatcggcgg cacggtgatc cgcactttac tgcctttcgc
     7021 cgttactcgt gcccgccagt tttcaatttg gctccagtcg gcatcaccgt tcaggtagat
     7081 ttcaccctgc tgggtccgta ctgtaccggc aagcgtcgag cgcataccgt taaagttgac
     7141 cgcaagctgg ctcggctgca tatcaaacgg cataaagttg ccgtcgatat ccacacccgt
     7201 aacctgaagc tgaccaaaca actgcgggct ttgcacatca ccacccagac gcaagttggc
     7261 actcaccatc cccgctgctt tttccccacg ggtaaagatg gggtttatca tcgcaaggtt
     7321 gaagttacgg atattgacgt tgccaccaag attacggcgg ccttgcggat cggtcacctg
     7381 cacctgtcca tcaaactggc cgttattggt caggcggatg gtccagccca attcggcacg
     7441 gttgttacgc aattccgccg tcagattcag tgtctgaaac gccaccggca gcgccgcatc
     7501 gttgacggtt tgcgttacct gcacgttacg ccccgaaagg gtgatactgc cctgcggcag
     7561 cccctctttc gtggtgtccc aggcaacatc cgctttaccc gtgaagatac cgctggcctg
     7621 agtggtttct ggcataaacg gtttcagcat ggcgaggtcg aagcggttga gattcaccac
     7681 cgcacgccct tcggcccccg catcgatagt ttgcggcacg cacagttccg cattcgggtt
     7741 aagccagcag tgtggcccga tgctgatttt ttgctcctta ttgcggtaat ccagcgcaat
     7801 atcgcgggtc agcgaccacg ggccgaccgg cgtctggaag cgggtattgc taagagttcc
     7861 cttccagcgt tcttctttgc gatcaaaact tcctgccaga ttaagctgcc cggagacagg
     7921 ctcgccctga atccgcaact gtagctcgtg ctgcttttcg ctgcctttgg cattcagggt
     7981 gacgaggttg atatttacat ccggctgcga aatttgctca acgcgtacgt cgagtttccc
     8041 ggcgatctga tcggtggatt taatgtcgcc ttccacgcga acctgcgcca cggaaagttc
     8101 ctgccagcgc aggccgcgcg cggtgatatc tgccagtagt tgtggcgctt ccaccgtgcc
     8161 gcgtactttc accagccctt tcgctgtacc gccaagcccc ggcagcgcgt tatccagccc
     8221 cggcgcgttg atggtggcat caagattgag atcttttacc cccagctcgc ctttcacttc
     8281 ggcactgttt ggcccgagtt ccagatgaag cccagggatc atccactgca tataactgtt
     8341 gcctttcagc gtgccgtcaa cgttcacttt gttctgtttg acgttaccgg tcagcttcag
     8401 ttctggcacc tccatctgcc aggtgccgcc gtacaggcta ccgcgggttt taatcaagcc
     8461 attgagtttc gacggccact ccgggaactc tttggcggtg ttaatgccat taagcgttag
     8521 ctcaccgcgc caactaatgg cctgctgcca gtcgagcaac gccttgagtt cagttttccc
     8581 ttccagcgcc gcgacggtga gtttgtcgag attgacctgc tgttcattac ctttggcatc
     8641 aagggtaatg gtggctggcg ggatctcctg tcccttcact gccgtacgca tagagagcgt
     8701 gtaatcggtc attttgccgg taagtttcag tttcagatca tccgcctgat actgcttctc
     8761 accagtgaac ggccagtaaa gctgtttgct gttcacttcc acgttgagtg gcaatccggc
     8821 ttccgccagt cgcgtctggg cgcgtaaatc catatccacc ggaccggaaa ggttaacgcc
     8881 tatctccagc tgttcgcgca gcgcgccgcc cactttcagc ttcacctttt cacctttcaa
     8941 cggctccacg ttcagtgtgc tgttgagggt gatatccacc ggccagttgt ctgacagctg
     9001 cgccgtgccg ctggcgttga cgatcccctg gttggaatcg atatccaggg cgtccagttt
     9061 agtattgccg tcaatgctgc tcactttcag cagcatggtg cgcacggtga tgtccgtgtc
     9121 gcccgtcacg cgcagctgct cgcccttaaa ctcttcaatg ttcaggttaa gcggcaaatg
     9181 cacgtcggtc atttccggca ataccgggcg agaaaagaga tctttcagcg tttcgccgag
     9241 cggcttttcc tccggctgcg gattttcaat tttcggttcg accacttctt cctgcgccac
     9301 ttccaccact ttcggcagag caatcagcag gcctttcagc gacgtcggtt tcagggtcag
     9361 ggttttctcc tgccagttca ggccggaggt gaagtccatc accgacaccg tggtgtcatc
     9421 aatcttgatg ttgacgttgt ccagcgccac ccgtgtcagg gtgatgggat acggcgtgga
     9481 gagatccagc ggaccgctat cttcctcttc ttcaacctgt tcagaaggag gcattttttt
     9541 actgtcgatg ttgacctgaa tgtctttcag cgccaggtca ttaatacaaa cgctactgtt
     9601 ccacaggcac tcaagaccga cccccagatg cagattaccc gcttttaccg ccacgcctag
     9661 ctgctcataa cgaacgtcag acaaggtgag atcgcgccag ccgccggtga ccttgccaat
     9721 atccagtcct ggcacccagc gatccgccgc tttaaataca agatgcaggc cgctggtggt
     9781 gcccaccaga aacgccaccg atcccagcaa cagtaagata acgataacca cgccgaggct
     9841 gatttttttc cataaactca taattctggc cccagaccga tgtaaaactg taacccgtgt
     9901 tcgtctttat ccgcgaccgg tacggcaaaa tcgagtttga ttggcccgac cggcgattcc
     9961 cagcgtacgc cgaccccggt accggtttta aagtcgctgc ggcgaatatc gcttaccgct
    10021 tcgccactat cgacaaacac cgcgccccac cattttccgg tcacgttgta ctggtattcc
    10081 agcgatccgg ttatcaactt cgaggcccct ttcaggtcac cgttggcgta tttcggggcg
    10141 atagatttgt atttgtagcc gcgaatactg cgatcgcccc cgacgaagaa acgcagatcc
    10201 ggcggtactt tgtcgaaatc accggtttca atccagccca gcgtgccgcg tgtcacaaag
    10261 cgatggcgat cgtacagtgt gcggatccag acgtgctgcg cctggaaaac ggagaaatcg
    10321 acatctgaac cccaggccgt gttggagtag tcgatagagt agcgttgcga gtctccccag
    10381 gttggcatca ggccaccacg agaacgcgtg cggctaatca tcaccccagg ataaaacagc
    10441 atcgtggtgt tggtaatttc accctgggta aagtggtcga ggctccagcg caggttaatg
    10501 gcacgctgcc agccgctgga gagatcccag tagcgagaag ccaccagcgt agtggagtca
    10561 gattcggtat cgttcaggtc agtgcgctta aaaccgccct gcaccaaata atattgttcc
    10621 agtggattct tcagcagcgg cattttatag ctgaagtcga gggtctgttc cggcgcggaa
    10681 atactggtac tggtggtcaa actgtgaccg tatgagttca tccacggctt tttccacgtc
    10741 gctttcacgc gcggtcccac gtccgtagag taaccgaccc cggtttcgat ggtgttttct
    10801 gttcgcggcg aaaccacgcc cgtcaatggt aatactttcg tttcgcgcgc tttatcaaat
    10861 tgtggagcca ccaccaccga gttaaaccag ccggtagcag aaagtcggcg gttcagttcc
    10921 gccagatctt tcgattcata ctcatcgccc tctttaaacg gcaccagatt ttgcaggtat
    10981 tcatcgcgga tttgtgatcc ttcaaaggtc acatgcccaa agcggtaacg ttcgccactg
    11041 ttataatcaa tatcccagaa ggctttatgc aggccgagcg caatgcccag ctgcgctttg
    11101 gtaaattcgc tatcgaaata acctttacgc aacgcaatac tggttaagga ctttttgaaa
    11161 ttttcataat cgccctggtt cagtaccgta ccaatagccg ggcgagtatc gagcaatttc
    11221 aaatagtctt tatcggtccg cgcgccgccg cgcaatacca catcggtgcc gccaattaac
    11281 accggcacac ctggcgtgac tttggcgatc aatacctgcc gccctttctt tggcggtgga
    11341 cggagatcaa attcaatggt cggctggtaa taacccagcg ctttcagacc ttcacggatg
    11401 gcatcatcga cgcgtgcgcg aaagcgacgg tctggcgtca cttcatcact ttcaatcgta
    11461 gaaagctgcg cacgaacgtt cttttccagc tgtcccgata acccctcgac ctgtagacgg
    11521 acgttcgcgg cgacggcaga tccgcttaag cagagtaagc ttacacagca taactgtcgg
    11581 atatagcgca cattttctcc tgaatatcct tttttcctgc ccctggaacg ccgttaaacg
    11641 gcttaacaaa aatccagtaa tatggattaa aaagcagact aaaccccaaa tatttcttat
    11701 gttttacttt agacctattc acggtggtta ttgtgtgcaa atacgcctct tgttacaacc
    11761 ttaaccccaa tgaccgattt tcgggagagc gacaccatga gtttatttga taaaaagcat
    11821 ctggtttccc ccgccgatgc cctgcctgga cgtaacaccc cgatgcccgt agccacgctg
    11881 catgcggtca acggtcactc aatgaccaat gtacctgacg gaatggagat tgccattttt
    11941 gcgatgggtt gtttctgggg tgtggagcgt ctgttctggc agttacccgg cgtttacagc
    12001 accgccgcag gctataccgg cggctatacg ccaaatccga cttatcggga agtgtgctcc
    12061 ggtgatacgg gtcatgccga agcggtacgc attgtttacg atccttccgt catcagctat
    12121 gagcagttgc tacaggtatt ttgggagaat cacgatcccg cccagggaat gcgtcagggc
    12181 aatgaccacg gcacgcagta tcgttcagcg atttatccgc tgaccccaga acaggatgcc
    12241 gcagctcgcg ccagtctgga acgttttcag gcggcgatgc ttgccgccga tgacgatcgt
    12301 cacatcacca cggaaatcgc taacgccaca ccgttttatt atgccgaaga tgaccaccag
    12361 caatatctgc ataaaaaccc atatggttat tgtggaattg gcggaattgg cgtctgtctg
    12421 ccgccggaag catagcgtta cgggtacaaa tgtagtttgt tgataaagtg ctctttgtct
    12481 atgcctgatg cggcttaaac gacttaccag gcctacaaaa tcgcccaaat tcaatatatt
    12541 gcaaggactg cgtaggcctg atcggcatag cgcatcaggc agtattgcat ttatcagcag
    12601 tctgaatttt aaccctctgg cgactttaca gtaccttacg ctatactagc cactgaaaat
    12661 gccggttcac tttcttcgaa tcggctttca atgtgtattt cacacaaatt aatcaacttt
    12721 cccttccgag ggtctggcct gaaaggtcgg ataagatatg ttaaacagta ttttagtcat
    12781 actctgcttg atcgctgtaa gtgcgttctt ctcgatgtcc gagatctcgc ttgccgcctc
    12841 acgcaaaatc aaacttaaac tgctggctga tgaaggcaat ataaatgccc aacgcgttct
    12901 gaatatgcag gaaaatcccg gcatgttctt taccgtggtc caaatcggtc tgaacgcagt
    12961 ggcgattctc ggcggtatcg tcggtgatgc ggcattttct ccagcttttc acagcctgtt
    13021 ctcccgctat atgtcggcag agctctctga gcaactgagc tttattctct ctttctcgtt
    13081 agtgactggc atgtttatcc tgtttgcgga tttaaccccg aaacgcatcg gtatgattgc
    13141 gccagaagcg gtggctttgc gtatcatcaa cccgatgcgc ttctgcctgt acgtttgcac
    13201 cccgctggtg tggttcttca acggcctggc gaacatgatc ttccgtattt tcaaactgcc
    13261 aatggtacgt aaagatgaca tcacttctga tgacatctac gcggtagtgg aagccggtgc
    13321 gctggcgggc gtgttacgta aacaggaaca cgagctgatt gaaaacgtct ttgagctgga
    13381 atcccgtacc gttccgtctt caatgacacc gcgtgaaaac gtgatttggt ttgatctcca
    13441 cgaagatgag caaagcctga agaataaggt ggcggaacat ccgcactcta agttcctcgt
    13501 ctgtaatgaa gatattgacc acatcatcgg ttatgtcgat tctaaagacc tgctgaaccg
    13561 cgtgctggct aaccaaagcc tggcactgaa cagcggcgta caaattcgca acacgctgat
    13621 tgtgccggat acgttaaccc tttcagaggc gttggaaagt tttaaaaccg caggtgaaga
    13681 cttcgcggtg atcatgaacg agtacgcgct ggtggtgggg atcatcaccc tcaacgacgt
    13741 gatgaccacg ctgatgggcg atctggtcgg tcaggggctg gaagagcaga ttgtcgcccg
    13801 tgatgagaac tcatggctga ttgacggcgg caccccaatt gacgacgtca tgcgcgtgct
    13861 ggatattgac gagttcccgc agtcgggcaa ctacgaaacc atcggcggct ttatgatgtt
    13921 tatgctgcgt aagatcccga aacgcaccga ttcggtgaaa ttcgccggct acaaatttga
    13981 agtggtggat atcgataact accgcatcga ccagctgctg gtgacccgga tcgacagcaa
    14041 ggccaccgcc ctttcgccaa aactgcctga cgctaaagat aaagaagaaa gcgtcgcgta
    14101 acccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc atagcacttt
    14161 ggttaagcca tctctgtttg cagacgcata acctgacggt taacttcgga catcactgac
    14221 aaatgcagct tatccttcac ttttgggata agaatcttac ccttatcaaa ttcaaaagcg
    14281 ccaatgtcct tgatgtataa ccgtccacgg aacaggatct ttacgtactt cgccacctga
    14341 agtgggttgt accgttggaa aattttcatt gttatctcct gctgagtatt acgcctgtgc
    14401 ggtgccacaa tcggcccaac tattatgagg cgcaaatttt aatgcttagt gactatagac
    14461 tatccgggca atgtttccac cgcgtataac tttttttacc taaaggttac aattattcag
    14521 aattatcttt ttaccgaagc gcggtcttca gtataagcat tcatttttca tatgatttgt
    14581 gcgcttgacc gcaaactggc atcacacttg cgggaaattc gataaatagc acatatgatt
    14641 aaaactcaga cccaagtggt cggatcacct gcatatcata agaaggaaac accatgaccc
    14701 tacgcaagat tctggcactc acctgcctgc tgttgccgat gatggcttcc gcacatcagt
    14761 tcgaaatcgg tcagcgagtg ccgccgattg gcattaccga tcggggcgag ttggtgcttg
    14821 ataaagatca gtttagctac aaaacctgga acagcgcgca gttagtggga aaagtgcgag
    14881 tactgcaaca tattgctggt cgcacctctg caaaagagaa aaacgcgacg ctgattgaag
    14941 cgattaaatc agcgaagtta ccgcacgatc gttaccagac caccaccatt gttaacaccg
    15001 acgacgcgat tccgggttcc ggcatgtttg tgcgcagcag tctggagagt aataaaaagc
    15061 tttatccctg gtcgcagttt attgtcgata gcaatggcgt cgcgcgcggt gcctggcagc
    15121 tggatgaaga aagttccgct gtagtggtgc tggataaaga cggtcgcgtg caatgggcca
    15181 aagacggggc gctcacaccg gaagaggtgc agcaagtgat ggacctgctg caaaagttgc
    15241 tcaaataatg gcaataacat aaattctgga aaaataattt tcccctatcg ttatctatgg
    15301 gataggggaa ttaagtctct gagttcttac accgaactaa tactggtaac gctagttgct
    15361 cttttttaaa cgcctcaatg gtcgatttat atttctcgca aatcttttct ttggtaatat
    15421 tactatctgc ggccagattc gcacttacta tcgcatccac ttgttcggct aattcgactt
    15481 gttggcaacc tgcattaacc agaaaattat aaataagaga gtaatcttca tcctccaaaa
    15541 caacagagga accattacga aaacgttcat cccctccata gattttaagc gcaaagccca
    15601 aagattctat gactatggca aaattatcat caattatttt ttcgtaaata taccatccat
    15661 cgcattcttt aattccaccg cctggtattt ggagttcaaa tttaccccgt tttaatttgc
    15721 ttactacatc ctccaataag gcataaccga aagatacgtc accaaacgcc tggcgaatat
    15781 aacctttaat cctttttggt atgtcagact gttttttctg caaaatatca caggcataca
    15841 atcttctgtt ttgttctaac ggaaactcgc ggtttttgaa atccatgaat gagttatact
    15901 catttattgt tatgggaatt tcttcgtatt caaacccact gcctcgcaat gttattgtat
    15961 attcagcacc gttatcttta aggctgaaac tgataagaaa attatccatg gcattaacag
    16021 tttcattttt tgatgaaagc acttgataac tatttttatc aatctgtgtt gcgcaacgaa
    16081 aggactcccg caaagttaac agactatcat aattcgaaac ccaatctttt ttcttaaatg
    16141 catgctgaat attattaaaa aaatcgtttg aatgtgtttt tgtatgcgat gactttacaa
    16201 ataactccac cgcaccaata aaattattgt gtctgatcct gggtaacata tggctacctt
    16261 attttatttg gttattttat gtcgccatta aatatctcat tatagaagta aactatcaaa
    16321 aaacgctcat tattaaaatg taacttacta gcaattgaat ttttgcactt taaatacaat
    16381 ctctttttat ttataacttt ttaggttggg ataaatcagg atggcagaat caggaaatac
    16441 actgtttctg ccatctgaat ttagtaaatg gacactctga accccggatt caggaacgac
    16501 tcgcgcggag tgtaatccag cggtttaccc tgccagtcgt gaacgtgcgc tccggcagct
    16561 gcagccacag catgtccggc ggcggtgtcc caaatattcg ttggtccgaa gcgcgggtac
    16621 agctgcgcct gtccttccgc caccaggcag aatttcagcg aagagccgat ggacgtggtc
    16681 tgatgttcgc caagctgttg cagatactct ttcagctccg catctgcatg ggaacggctg
    16741 ataaccacca gcggcgggcg cgcatcgcgg acctgaatca gcttgcgcac accgcactct
    16801 tctttccagg ccttgccttc tgccgcgctg tacattacgt tcattaccgg cgcatacacc
    16861 acacctaaaa tcggtttgcc atggtcaatg agcgcaatgt taacggtgaa ttcgccatta
    16921 cgtttaataa actctttagt accatccagc gggtctacca gccagtaacg ctgccagtgc
    16981 tgacggactt cccaaccggg aggatcttct tcagaaagga ccggaatatc cggtgccagc
    17041 gtacgtaaac cgtccatgat aacggtgtga gcggcaatat ccgctgccgt taccggagaa
    17101 ttgtccgctt tgctgacgac gtccatcggt ttcgtcccgt cgtagacctg cataatggca
    17161 tcgcctgcat tccgtgcaag ctggcatact tgatctaaca tttctccacc tcgtctctgt
    17221 gagcggtgtt aacttattgt tttacttata ccctatcgtt aatgaatgcg ccaactgtga
    17281 tagtgtcatc attttcaaag cgtaaaattg tggcattctt cactgttcta taagtaaggc
    17341 gtttattctc ccttttcttt cgtattcccg atgataaaag gatgtccctg atgattaagt
    17401 ttagcgcaac gctcctggcc acgctgattg ccgccagtgt gaatgcagcg acggtcgatc
    17461 tgcgtatcat ggaaaccact gatctgcata gcaacatgat ggatttcgat tattacaaag
    17521 acaccgccac ggaaaaattc ggactggtac gtacggcaag cctgattaac gatgcccgca
    17581 atgaagtgaa aaacagcgta ctggtcgata acggcgattt gattcagggg agtccgctgg
    17641 ccgattacat gtcggcgaaa ggattaaaag caggtgatgt tcatccggtt tataaggcgc
    17701 tgaatacgct ggattatacg gtcggtacac tcggcaatca tgaatttaac tacggtctgg
    17761 attacctgaa aaatgcgctg gcgggagcga aattccctta tgtaaatgcc aacgtcattg
    17821 acgccagaac caaacagcca atgtttacac cgtatttaat taaagatacc gaagtggtcg
    17881 ataaagacgg aaaaaaacag acgctgaaga ttggctatat tggcgtcgtg ccgccgcaaa
    17941 tcatgggctg ggataaagct aatttatccg gaaaagtgac ggtgaatgat attaccgaaa
    18001 ccgtgcgcaa atacgtgcct gaaatgcgcg agaaaggtgc cgatgtcatt gtcgttctgg
    18061 cgcattccgg gctgtctgcc gatccgtata aagtaatggc ggaaaactca gtttattacc
    18121 tcagtgaaat tccgggcgtt aacgccatta tgtttggcca tgctcacgcc gttttcccgg
    18181 gtaaagattt tgctgatatc gaaggggctg atatcaccaa aggcacgctg aatggtgttc
    18241 cggcggtaat gccgggcatg tggggcgatc atcttggggt ggtcgactta caactcagta
    18301 atgacagcgg taaatggcag gtgacgcagg cgaaagcgga agcacggccg atttacgaca
    18361 tcgccaataa aaaatccctc gcggcggaag acagcaagct ggtagaaaca ctcaaagccg
    18421 atcacgatgc cacacgccag ttcgtcagca agccaatcgg taaatctgcc gacaatatgt
    18481 atagctatct ggcgctggtg caggacgatc cgaccgtgca agtcgtgaac aacgcgcaaa
    18541 aagcgtatgt cgaacattac attcagggcg atccggatct ggcaaaactg ccggtgcttt
    18601 cagctgccgc accgtttaaa gttggtggtc gcaaaaatga cccggcaagc tatgtggagg
    18661 tggaaaaagg ccagttgacc ttccgtaatg ccgccgatct ttatctctac cccaatacgc
    18721 tgattgtagt gaaagccagc ggtaaagagg tgaaagagtg gctggagtgc tccgccggac
    18781 agtttaacca gattgctcct aacagcacga aaccacagtc actcatcaac tgggatggtt
    18841 tccgcactta taactttgat gttattgatg gtgtgaatta tcagattgat gttacccaac
    18901 ccgcccgcta tgacggcgag tgccagatga ttaatgccaa tgcagagcgg attaagaacc
    18961 tgacctttaa tggcaagccg attgatccga acgctatgtt cctggttgcc accaataact
    19021 atcgcgctta cggcggcaaa tttgccggta cgggcgacag ccatatcgct tttgcttcac
    19081 cggatgagaa ccgctcggtg ctggcagcgt ggattgctga tgagtcgaaa cgtgcggggg
    19141 aaattcatcc ggcggcagat aacaactggc gtttagcacc gatagctgcc gataagaaac
    19201 tggatatccg tttcgaaact tccccgtcag ataaagccgc agcgtttatt aaagagaaag
    19261 ggcagtatcc gatgaataaa gtcgcgaccg atgatatcgg gtttgcgatt tatcaggtgg
    19321 atttgagtaa gtaaaacact tctttttcgg cctataaatc aaccgcatcc ggcatttatt
    19381 ggcggatgcg atgctgccgt atcttatcca ccctacaagc catgaaccgt agaccggata
    19441 agcgcagcgc atccggcagt tatgccgcac gttcatcccg caccgccaac acctcgggca
    19501 aattcaactc aatccagtcc gccagtgcag caaccttttc gctcacctgc tcgcccagcg
    19561 gcgtgaggct atattccaca tgcggcggca ccaccggata cgcgatacgg ttaagaaaac
    19621 catcctgttc taacgcctgt aacgactgcg caagcatctt ttcactcacc ccaccgattt
    19681 tgcgccgcag gtcgctaaag cgatgagtac cttcgcgtag cgccaccaga atcaacaccc
    19741 cccaacggct ggtgacgtgt ttcaacacct cgcgcgacgg gcactgttcc gcaaagagat
    19801 taccctcttt cagttgttgt gacaggctaa cctgactcat ttcatactta cctttttgca
    19861 cgtacttact aaaagtaagt ttaggtgtta gcgtatttaa acacaagaca aaacgatgga
    19921 gacttcccat gatcgctatt actggtgcca ctggccaact tggtcactat attattgaat
    19981 ccttgatgaa aacggttcct gccagccaaa tagtggctat cgttcgtaat ccggcaaaag
    20041 cccaggccct ggcagcacaa ggcattaccg tgcgtcaggc tgactacagc gatgaagccg
    20101 cactgacatc tgcacttcag ggagtggaaa aactactgct gatctcttcc agcgaagtgg
    20161 gtcaacgtgc cccgcagcat cgtaatgtta ttaatgccgc aaaggcggct ggcgtgaaat
    20221 ttatcgctta taccagcctg ctacatgcag atacctcccc gctcggcctc gccgatgagc
    20281 acatcgagac ggagaaaatg ctggctgatt ctggcatcgt ttacaccctg ctgcgcaacg
    20341 gctggtacag cgaaaactac ctcgccagcg ccccggcagc actggaacac ggcgtattta
    20401 tcggtgcggc tggcgatggc aaaatcgcct cagcaacgcg ggcagattat gcgacagctg
    20461 cggcacgcgt gattagcgaa gccggtcacg aaggcaaggt ttacgaactg gcgggcgata
    20521 gtgcctggac gttgacacag ttagcggcag agctgaccaa acagagcggc aaacaggtta
    20581 cctatcaaaa tctgagcgaa gccgatttcg ccgcggcact gaaaagcgtc ggactgcccg
    20641 acggactggc ggatatgctg gcggattctg acgttggcgc atcgaaaggt ggtctgtttg
    20701 ataacagcaa aacgcttagc aaattgattg gctgcccaac gacaacgtta gccgaaagcg
    20761 taagccatct ttttaatgtt aataactagt taattaaagt ggcatcctcc cgcatcctct
    20821 ctgataatga cgggatgccg ggagcaatca tgtctgcttc ctgaacgttt cttctgacag
    20881 accaatggat gccagtaatg attagcggcg tgctgtacgc cctgttagca gggttgatgt
    20941 gggggcttat ttttgtcggg ccgttgatcg tgccggaata cccggcgatg ttgcagtcga
    21001 tggggcgtta tctggcgtta gggttaattg cgctacccat tgcctggctg ggacgcgtgc
    21061 gtctgcgtca gttggcgcgt cgggactggc ttaccgcctt gatgctcact atgatgggca
    21121 acctcattta ttacttctgc cttgccagtg ccattcaacg tactggcgcg cctgtttcca
    21181 cgatgattat cggcaccctg ccggtggtga ttcctgtctt tgccaatctg ctttatagcc
    21241 agcgcgacgg caaactcgcg tggggaaaac tcgccccggc actgatttgt attggcatcg
    21301 gcctggcgag tgtgaatatt gctgagttaa accacggact ccccgatttt gactgggcac
    21361 gttatacctc tggcatcgtg ctagcgttag tttccgtggt ctgctgggca tggtatgccc
    21421 tgcgcaacgc ccgctggctg cgggaaaatc ccgacaaaca tccgatgatg tgggcgacgg
    21481 cgcaggcgct ggtcacgctg ccggtttctc tcatcggcta tctcgtcgcc tgttactggc
    21541 tgaatataca aacgccggac ttctccttac cttttggccc ccgtccgctg gtgtttatta
    21601 gtctgatggt tgcgatagcc gtgctttgct catgggttgg cgcactctgc tggaacgtcg
    21661 ccagccagcg attaccgaca gtgattctcg ggccgctgat tgttttcgaa acgctggcag
    21721 gtttgctgta caccttttta ctccgccagc aaatgccgcc gctaatgacg ctgagcggta
    21781 tcgcgctgtt agtgattggc gtggtcatcg cggtcagagc aaaaccagaa aaacctttaa
    21841 ctgaatctgt ctcagaaagt tgacatgctg gcagtgagtt aaataagcct ctgctacgta
    21901 agggttatag cttttgcctt aaaggtgtat ttaaaataca tcttatctta ttaagaatga
    21961 ggtatcagct atggcttatc gcgaccaacc tttaggtgaa ctggcgctct ctatccctcg
    22021 cgcttcggct ctgtttcgta aatatgatat ggattactgc tgtggcggta agcagacgct
    22081 ggcgcgcgcg gcggcacgta aagaactgga tgttgaggtc attgaagctg aactggcaaa
    22141 gcttgctgaa caaccgattg agaaagactg gcgtagcgcc ccgctggcag aaatcattga
    22201 ccatatcatc gtgcgctacc acgatcgtca ccgcgagcag ctgccggagc tgatcctgca
    22261 agcgactaaa gtcgagcgcg ttcacgccga caaaccgagc gtgccaaaag ggctgacaaa
    22321 atacctgacc atgctgcatg aagagctttc cagccacatg atgaaagaag agcagatcct
    22381 cttcccgatg atcaaacaag gcatgggcag ccaggcaatg gggccaatca gcgtaatgga
    22441 aagcgagcac gatgaagcgg gcgaactgct ggaagtgatc aaacacacta ccaataacgt
    22501 cacaccgccg ccagaagcct gcaccacctg gaaagcgatg tataacggca ttaatgaact
    22561 gattgatgac ctgatggatc acatcagtct ggaaaacaat gtactgttcc cacgcgccct
    22621 agcgggtgag tgatctaaaa gctggatggc attgcgccat ccagcatgat aatgcggtta
    22681 tttccgcagt tcagcagccc gcttcttacc aataaacagc cagcccaacc ccagcgcgat
    22741 aaaccacagc ggggtaacca gcagcgcctg gcgagtgtca tcttccagtg tcagcaacac
    22801 gaccacgaac acaaagaacg ccatacatac ccagcacatc agcttgccga gcggcatctt
    22861 gtagatcgac ttttcatgca gatgaggacg ctgtttgcgg tacaccaggt aagagcaaag
    22921 gataatcgtc cagacgaaca taaacagaat cgcggaaacg gttgtaatca tcgtgaacgc
    22981 gccaatcacg ctaggattca catacaacat caccacgccg ccgagcagac agatacacga
    23041 gaaggtcaac cctttcgcgg gtactgcgcg cttagaaagt ttagcgaacg ctttcggcgc
    23101 aacaccttcc tgcgccagac caaacagcat acggctggta gagaagacac cgctgttagc
    23161 ggaagacgcc gcagaggtca gcaccacaaa gttgatcacg ctggcagccg caggcaaacc
    23221 taccaacaca aacagctcaa cgaacgggct tttctccggg actaccgaac tccacggtgt
    23281 cacggacata atcacaatca gcgcgaagac gtagaacata atgatacgga tcggaatgga
    23341 gttaatcgcg cgtggcagtg atttctccgg atctttggtt tccgcagctg ttgtacctac
    23401 cagctcaatc cccacgaaag cgaaaaccgc tatctggaat ccggcaaaga agccacttaa
    23461 acctttcggg aaccagccgc cgtcattcca caaatgcgcg aatgacgctt ccacaccggt
    23521 cggtgactga aagtgcatcg ccaccatgac caggccgacg acaatcagcg acacgatggc
    23581 gacgattttg atcatcgcaa accagaactc catctcaccg aacattttca cggtggcaag
    23641 gttgagcgtc agcagcagca ctatcaccgc cagcgaggcg acccagtcgg agagatcggg
    23701 gaaccagaac tgagcataag ccgtgatcgc caccacgtct gccataccgg ttacaaccca
    23761 gcagaaccag taagtccagc cggtgaaata tcccgcccac ggccccagta aatcggaagc
    23821 gaagtcacta aaagatttgt attccagatt cgaaagcagc aattccccca ttgcccgcat
    23881 cacgaaaaag agcataaaac caatgatcat ataaacgaaa atgatcgacg gcccggcaag
    23941 gctaatcgtt ttgccggacc ccataaacaa cccagtacca atggcacctc caatggcaat
    24001 aagctgaaca tgtcggtttg tgagattgcg ccgtagcgac tgttcagccg gagcctgatc
    24061 atcggcaacg acttttacct gatctaccat gttttttctt cctgtacctg tctgtgttgt
    24121 tcaggctcta cggcctttag tcagtctatg atacgacgaa tctgtatcgg gactcatcga
    24181 tattaggtaa caatacgcgg gatgaatact aagatttaga tctaatgtta attttatgtt
    24241 taaagtgagt gtcatatcac ggtgataacg cgaaacagct cacaaaaata cacgcttacg
    24301 gggtctttgc aacacaaaat agcgccgttt ggcaaactga agggtttatt gctgaatgcc
    24361 tgctcccctc tggttcgagg ggaacagaat gcgctgctta gaggatttcc agcagttcga
    24421 cttcaaacac cagggtgctg aacggaggga tggatgcacc tgcgccgcgc tcgccatatg
    24481 ccagttcctg cgggatagtc agttcccatt tagaacctac cggcatcaga gtcagtgctt
    24541 caatccagcc agggatcacg ccattaaccg ggaattcagc gggttcacca cgagcaacgg
    24601 agctgtcaaa cacggtgccg tcgatcagtt taccggtgta atgaacacga acgcggtcgg
    24661 tacgtgccgg aattgcgcct tcaccctggt tgatcacgcg gaattgcagg ccagattcgg
    24721 tgctattcac accttctttt ttggcgtttt cttccaggta tttcacgcct tcagcagcca
    24781 tcgcctggaa acgctgacga cgaacggcat cggcgcgttc gtggatttca cgcagcgcgc
    24841 gatgcaccac atcaaccggc acagccggat gtttgccttc cagcgcatcg gcaatacctg
    24901 caaccagtgc ttctggcagc agcccttcca ggccagattc actcagttgt tgccctacct
    24961 gcaaaccaat gccgtagctt gcttgcgctt cgatggtgtc aaaagttggg gtggtcatcg
    25021 tatttccttt cttagggtat aaaagtagcg ggcagcatat cagccacact tctttgggta
    25081 aaactttgtc gcaggaatga tgacaaatcc cggggaaaaa gaaacaatag caatatcccg
    25141 tatgaatata ataaaggcgg gtttaccacg gacatcatag cgttagctta ttcataggct
    25201 atgattgagg aacaagacgt ggagcaggag gaaaccatgc ccgggcgctt tgaactaaaa
    25261 ccaaccctgg agaaagtctg gcacgcgccg gacaattttc gcttcatgga cccgctgccg
    25321 ccgatgcatc gtcgggggat cattattgcc gccattgtat tggtggtcgg atttctgctt
    25381 ccatctgatg atacgcccaa cgcgtcggtg gtgacgcgtg aagcgcagtt agatattcag
    25441 tcgcaatcac aaccgccgac agaagaacag ccgcaaacta cccaaacgca accattccag
    25501 ccagatagcg gaattgataa tcaatggcgc tcttaccgtg tcgaacccgg taaaacgatg
    25561 gcgcagctgt tccgcgacca cggactgccc gctaccgatg tctatgcaat ggcgcaggta
    25621 gaaggcgcgg gtaagccgct gagtaatttg caaaatgggc agatggtgaa aatccgccag
    25681 aacgccagcg gcgtggtgac gggcttaacc attgatacgg ggaataatca acaggtcctg
    25741 tttacccgcc agccggatgg cagttttatt cgtgcgcggt agatgaaggg tcaaccaaaa
    25801 gccagctaag gattgtctct ttgatgacct tacgtctggc ttccagtgcc tggggggaaa
    25861 ccatactgat atgataaact aggccaagcg tatgctggtt gatcaaataa tatcccccaa
    25921 gcgcagcaat attaatattc acctgcaatg ggtcgatacc tggcttaaaa atattgtatt
    25981 tcttcccttc atccaataag gattccatta actgcaagtg agcatggtta atttctaaga
    26041 gacgctgtga ttttgcataa tgcacacctt tactttgatt ttcactatgc acaattttta
    26101 aaaaccaagg attagcaata taataatccc atgtaaaatc aataattttc tcaatggctt
    26161 tttgtggcgg aagatctgag aaatcaaggg ccttttcttt ttgtcgaata tcgttccagg
    26221 tatattccag cacttcgata aataaatttt ctttattacc aaagtggtga taaaccgtct
    26281 gtttactaca ccctgctgcc tttacaatat tatctactct ggccccttca taaccatatt
    26341 cggcaaattc attaaccgca catagcagca atttttcctt caggctctgt acgttgctgg
    26401 tatcaatata cataacgctt ccccacgacc tcttcgtggt gactatcata acctgatttg
    26461 atttcagctt cagaaaaaag ggcaaaaact tgtactttca tcaggttatc aaatgtcctg
    26521 tgatcgccat cgcatatcaa ccaactagat ggtttatctt tttatcctcc agtcgcaagc
    26581 taatcatcac caaggaaaca aaattcaaaa aagagagcta ttgtatgaaa tccactcgtc
    26641 ctgttgccgt gattaccgga gccactcgcg gtattggaaa gggctgtgcg caggagcttg
    26701 ctcgtggcgg gtttaacctg ctgattaacg atcgtcccga tgccgacagc gtagaaaaac
    26761 tgcatatcac acaacaagaa tgtttggcgg aaggcgtgga agtgatttgc ttccctgctg
    26821 atgtcggtga tctatctctg catgaggaga tgctcgacgc ggcgcaaaac cagtgggggc
    26881 ggctggactg tctgctcaat aacgcgggga tttcagtgaa aaaacgcggt gacctcctcg
    26941 acctcgaacc agacagcttc gatcaaaaca ttgccattaa cacccgcgcg ccgttcttcc
    27001 ttgctcaggc attcagtaag cgcttactgg cacagccgaa accggaagcg gagctgccgc
    27061 accgctcgat tattttcgtc agttccatca acgccatcat gctggcgatg aatcgcggcg
    27121 aatacaccat tgccaaaacc gccgtttctg ccgctgctcg tctgtttgct gctcgccttt
    27181 gtaatgaaca aattggcgtt tatgaagtgc gccctggtct catcaaaact gatatgacta
    27241 ttccggcaac ggcttattac gacgaactga ttgccaaagg tctggtgccg tggggacgtt
    27301 ggggttatcc ggctgatatt gcttccaccg tccgcgcgat ggcggaaggt aaactgattt
    27361 acacctgtgg tcaggcggtc gcaatcgacg gcggcttaag tatgccgcgc ttctgaggct
    27421 gaatcatgag agatctgtta caacgcccgg atttattcag tatcaatacc gcgacactgg
    27481 gctataaaac gccgctaccc gccattattg atgcctgcgc cgcacgcggt attggcgcaa
    27541 ttgccccctg gcgcagggag ttgcagggtg aagatctcca gcaaatcacc cgccagcttg
    27601 ccgccagtaa catgagcgtt tccggtttgt gccgtagcac ttactacacc gcgccgacac
    27661 tggcggaacg caaactggcg attgatgata accgccgggc gctggacgac gcagcggtgc
    27721 ttaacgccgc ctgttatatg caggtggttg gcggtctgcc aacgggcact aaagatctat
    27781 atgaagcgcg agaacaagtg aaacaaggga ttcgccagtt gctgccacat tcgaaagatg
    27841 tcggcgtgcc aattgcgctg gagccactgc acccgatgac agctgccgat cgctcttgcc
    27901 tgtgcacgtt acgtcaggcg ctggactggt gtgatgaact cgatcccgat ggtgagtttg
    27961 gcctcggcgt ggcggtggat gtttatcacg tctggtggga tccggatctc gccagtcaga
    28021 tcctgcgtgc cggaaaacgc atcctcgcgt ttcatgtttc cgactggtta atgccgacca
    28081 ctgatctggt caatgaccgt ggtatgccgg gagatggcgt catcaatatt ccgtcaattc
    28141 gtcggctggt tgaaaacgcc ggatttaatg gtgctattga actggaaata ttctcgcctt
    28201 actggtggca gaaagatatt aacag
//
LOCUS       DAJTUI010000051        28163 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTUI010000051 DAJTUI010000000
VERSION     DAJTUI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.051"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(55..1347)
                     /gene="spaL"
                     /locus_tag="OO464_002309"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             complement(55..1347)
                     /gene="spaL"
                     /locus_tag="OO464_002309"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR6227437.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            complement(1351..1752)
                     /gene="spa15"
                     /locus_tag="OO464_002310"
                     /gene_synonym="spaK"
     CDS             complement(1351..1752)
                     /gene="spa15"
                     /locus_tag="OO464_002310"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR6227438.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            complement(1765..3825)
                     /gene="mxiA"
                     /locus_tag="OO464_002311"
                     /gene_synonym="virH"
     CDS             complement(1765..3825)
                     /gene="mxiA"
                     /locus_tag="OO464_002311"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR6227439.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            complement(3838..4905)
                     /gene="mxiC"
                     /locus_tag="OO464_002312"
     CDS             complement(3838..4905)
                     /gene="mxiC"
                     /locus_tag="OO464_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR6227440.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            complement(4924..6624)
                     /gene="mxiD"
                     /locus_tag="OO464_002313"
     CDS             complement(4924..6624)
                     /gene="mxiD"
                     /locus_tag="OO464_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR6227441.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            complement(6611..7243)
                     /gene="mxiE"
                     /locus_tag="OO464_002314"
     CDS             complement(6611..7243)
                     /gene="mxiE"
                     /locus_tag="OO464_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR6227442.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            complement(7373..7801)
                     /gene="mxiM"
                     /locus_tag="OO464_002315"
     CDS             complement(7373..7801)
                     /gene="mxiM"
                     /locus_tag="OO464_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR6227443.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            complement(7785..8192)
                     /gene="mxiM"
                     /locus_tag="OO464_002316"
     CDS             complement(7785..8192)
                     /gene="mxiM"
                     /locus_tag="OO464_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR6227444.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            complement(8185..8880)
                     /gene="mxiN"
                     /locus_tag="OO464_002317"
     CDS             complement(8185..8880)
                     /gene="mxiN"
                     /locus_tag="OO464_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR6227445.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            complement(8822..9349)
                     /locus_tag="OO464_002318"
     CDS             complement(8822..9349)
                     /locus_tag="OO464_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR6227446.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            complement(9346..10071)
                     /gene="mxiJ"
                     /locus_tag="OO464_002319"
     CDS             complement(9346..10071)
                     /gene="mxiJ"
                     /locus_tag="OO464_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR6227447.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            complement(10077..10370)
                     /gene="mxiI"
                     /locus_tag="OO464_002320"
     CDS             complement(10077..10370)
                     /gene="mxiI"
                     /locus_tag="OO464_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR6227448.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            complement(10383..10634)
                     /gene="mxiH"
                     /locus_tag="OO464_002321"
     CDS             complement(10383..10634)
                     /gene="mxiH"
                     /locus_tag="OO464_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR6227449.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            complement(10642..11757)
                     /gene="mxiG"
                     /locus_tag="OO464_002322"
     CDS             complement(10642..11757)
                     /gene="mxiG"
                     /locus_tag="OO464_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR6227450.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFFLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            complement(11766..12224)
                     /gene="ipgF"
                     /locus_tag="OO464_002323"
     CDS             complement(11766..12224)
                     /gene="ipgF"
                     /locus_tag="OO464_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR6227451.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            complement(12224..12586)
                     /gene="ipgE"
                     /locus_tag="OO464_002324"
     CDS             complement(12224..12586)
                     /gene="ipgE"
                     /locus_tag="OO464_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR6227452.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            complement(12596..14212)
                     /locus_tag="OO464_002325"
     CDS             complement(12596..14212)
                     /locus_tag="OO464_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR6227453.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            14526..16010
                     /gene="icsB"
                     /locus_tag="OO464_002326"
     CDS             14526..16010
                     /gene="icsB"
                     /locus_tag="OO464_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR6227454.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            16023..16412
                     /gene="ipgA"
                     /locus_tag="OO464_002327"
     CDS             16023..16412
                     /gene="ipgA"
                     /locus_tag="OO464_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR6227455.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            16428..17054
                     /gene="ipgB1"
                     /locus_tag="OO464_002328"
     CDS             16428..17054
                     /gene="ipgB1"
                     /locus_tag="OO464_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR6227456.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            17111..17578
                     /gene="ipgC"
                     /locus_tag="OO464_002329"
     CDS             17111..17578
                     /gene="ipgC"
                     /locus_tag="OO464_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR6227457.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            17584..19326
                     /gene="ipaB"
                     /locus_tag="OO464_002330"
     CDS             17584..19326
                     /gene="ipaB"
                     /locus_tag="OO464_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR6227458.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            19346..20437
                     /gene="ipaC"
                     /locus_tag="OO464_002331"
     CDS             19346..20437
                     /gene="ipaC"
                     /locus_tag="OO464_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR6227459.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            20488..21486
                     /gene="ipaD"
                     /locus_tag="OO464_002332"
     CDS             20488..21486
                     /gene="ipaD"
                     /locus_tag="OO464_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR6227460.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            21495..23396
                     /gene="ipaA"
                     /locus_tag="OO464_002333"
     CDS             21495..23396
                     /gene="ipaA"
                     /locus_tag="OO464_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR6227461.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            23400..23636
                     /locus_tag="OO464_002334"
     CDS             23400..23636
                     /locus_tag="OO464_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6227462.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            23853..24782
                     /gene="virB"
                     /locus_tag="OO464_002335"
     CDS             23853..24782
                     /gene="virB"
                     /locus_tag="OO464_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR6227463.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(25729..26508)
                     /gene="ipaJ"
                     /locus_tag="OO464_002336"
     CDS             complement(25729..26508)
                     /gene="ipaJ"
                     /locus_tag="OO464_002336"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR6227464.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(27108..27245)
                     /locus_tag="OO464_002337"
     CDS             complement(27108..27245)
                     /locus_tag="OO464_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227465.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            27861..>28163
                     /locus_tag="OO464_002338"
     CDS             27861..>28163
                     /locus_tag="OO464_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6227466.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFI"
BASE COUNT         9554 a         5755 c         4136 g         8718 t
ORIGIN      
        1 tgtgcttcaa ttaatttaac agaccgttga tatttatctt tgatttttaa aaccttatct
       61 aattgtttca ccaataagct ccattgtctg ctcataagta aaaccgagtc gataatcttg
      121 cttcagaaaa ctctcaacaa cagatatttt gttgtagatt ttatcctgag aggcattctc
      181 ccccggtttg tattcaccaa agtcaataat tgtccttagc tcttctattt cagaaagtag
      241 ctcccgaaat gcagcggcca taatacgatg tttttcatca acaacctgtg taaataccct
      301 gcttatactt tttaaggaat caattgcagg aaattgtcct ttttgggcta gatttctgct
      361 caaatatata tgtccatcta aaatggatct tacctcttca gctaatggat cagcaaaatc
      421 atcatcctcc aaaagaacag tgtaaaatgc agtaatagag ccacctgcct ttaactttcc
      481 tggcctttca agaagtctgg gtaagctatc aaaaaccgaa accggatagc ctcttctggc
      541 aggtgattct ccagcggcta aggccacatc acgtaatgct ctggcatacc ttgttaatga
      601 atcaataaaa agcgctactt tatgtccttc agtcctaaaa aattcggcta tagcagtggc
      661 tatatatgca gcattacaac gatcaaccga agagtaatcc gaagttgcat atactaaaac
      721 acacctgctt tttttctcag agtttttcaa ataatcaacc gtttcagtaa cctctcgacc
      781 tcgctcacca attaacccaa taacatatat atcagcacca ctatgttcaa tgagcatatt
      841 catgagaaaa gttttgccac aaccagctga cgcaaaaatc cccattcgct gtccttcacc
      901 acacgtgagt aaagaatcaa taaccttaat acctgttaaa aaaggcttct caattgcagc
      961 cctttcacta tatagcggag gagcattatc tacaggtcga taaagaattt cactgttatc
     1021 tgtaacagca aacttatcag taacctcccc taaaggattg actactgctc ccaataatcc
     1081 acggccgact tgcgtatgaa gaaactgggc agtgggctta atcaacgttt gccgtgaaag
     1141 tccacgagaa tttccaatca agcttaatat tgttttttca tcatgaaatc ctacaacctg
     1201 agctcttgca acaatttcat tactttcaat tccagcctga atgttacaaa tctcaccaat
     1261 cgaaacatcg ctaagacttg tttccaagat tggccccgag attctattag gaaaagataa
     1321 ttgagtgagc aattttgtat agctcattga ttataagacc ccatttaaga tttccatcct
     1381 ctgataaaac tcatgcagaa tctctgcaaa aacaatccca tcgtgaacgt agtcatcttt
     1441 aataacaact cttaactgta agtactcatc agaacgatga agctctacca gctcattaat
     1501 gctataacta aagttcatta acattaaatt taatatgtta taagcagatg actgaagttt
     1561 tacatcactt ggtgcatcaa aattagccca taacataacc tgctcattga caagagctat
     1621 attaatagca ggcatactat ccaaagaaat ggtaatcgct gaatggctgt ctaaatctgt
     1681 aataattgat ggaggacaac caatcgtgaa aagactatct ctaactaatt gaactaaatt
     1741 aatgttactc atatttaaac ctcactaaat agtctttaat acattaatgg tatatgcttc
     1801 atcaatctca gcatacgata taacgagtat actcttaaat ctgttatcta taagtctttt
     1861 aacaaaccta cgtatatcta ctgataccaa aagaacgaaa tttttttttg catttctcaa
     1921 ttctctcaaa gcatgtgcta aagtttccat tacctcatcc gaaacctcta tatccatatt
     1981 caagaaagag ccaccagagg tttgccttat cccttttctt attgcatcct caatatatcc
     2041 ggaaagcatc acaactttaa tctcaccaga aacagctatt ttactacaaa tatacctaga
     2101 aagtgatgca cggacatgtt caactaatgt tatgacatct ttttctctgg gagcccaaag
     2161 cgccaaagac tccataataa gttttaaatt gcgaacagaa atattttctc caagcaatct
     2221 ttgtaatacc tcagaaattc tctgtatagt cacatgtcgg aagacttcct ttaatagatc
     2281 aggataccga ttttcaaact gatctaacat attttttgtt tcttgtatac caaaaatctc
     2341 atttatgttg tttaataaag cttgtgacaa ctggtgataa aattcatctt gagcactttt
     2401 aattttagca tcaatattag ttagattttc tgtatatgaa acgtctaccc aagaaataac
     2461 acgttcatta tatgaagttg aaacaacagg aatacctaga gcatctatat ctccattttc
     2521 atctgtaata cacactttgt caaaatatat attaaaacta tcggcacgca cctcatttat
     2581 taacaaaaca atatcatcga ccttaagctc attgcttgtt ctatataaaa tagttggaag
     2641 cctgacacca taatcaataa agaactgact tcttatcctt tcaataagac cttccatatc
     2701 atttgcattt atcttatttt cggcaaataa taaaattaaa ggaacggttt ctgaactaat
     2761 agcatccaga ttttctatca ttgccaaaga tgagtcatgg ctattatcaa tatcaaatgt
     2821 accagtatag ccactagaat cagactcaga cagacttttt tctttttcta cgaccttttt
     2881 ataatagaat aaagccgtca aagtaactgc tatcaggaaa aaaacaaaaa acggaaaacc
     2941 tggcaacatc cctatcgcca aggcaagagc tgatgtaaca atgagaacaa aaggattccc
     3001 aaatatctgg gacattatgt tacgtcccat attatcgcta tcaccattaa ctcttgtaac
     3061 gataaatcct gcacttatag aaattaacag agcaggaatc tgagagacca atccgtcacc
     3121 aatagttagt atggtataag tagataatgc accagatagg gacattccat gttgactcat
     3181 accaacagaa atacctccta ttaaattgac aaaaataata atgatgccag caattgcatc
     3241 acctttaatg aacttcattg ctccatcaaa tgaaccatac aattgactct cacgttccag
     3301 aatactgcgt ctttctttag ctcctgcggc atcaataatt ccagccttta aatccgcatc
     3361 aatgctcatt tgtttccccg gcataccgtc aagagaaaag cgagcagcta cctccgcaac
     3421 cctttcagat ccttttgtta taacaatgaa ctgcacaaca gttactattg aaaaaataac
     3481 aaaacctaca gccagactat caccaatgac aaactggcca aatgtagtaa taattttacc
     3541 cgcatccgca tcaactaaaa ttagtcgact agtacttatt gataaagcga gacgaaataa
     3601 tgtcgttatc aacaaaaccg aagggaatgt agaaaaactc aatattcttt caatataaaa
     3661 tgatcccata aaaacgagaa tagcaagtac aatattaagc cctattaaaa aatcaacaag
     3721 gtatgtaggt aatggtatga tcaacatagc aatgatcata accataagca ctaatataat
     3781 taattcaggc ttagtactta cttgcttaag aaaagactgg atcactttta tctcctgtta
     3841 tctagaaagc tctttcttgt atgcacttgt gacaagctct cgtagaatat tgatcgcaat
     3901 ttctctttca ctttcacttt taaatatatc atgaggactt tcattaacag aagatagata
     3961 tgtttgatac actacagaat cctcatgact ggctcgtgat acttctataa cagaagtaag
     4021 aatttgctca ctttcacttg gatattgaaa aatatataat aaagttagca atagctgctc
     4081 tgcacttaaa ctgccatctt ttagcaagct tgaggattcc agtttcttca taaaaattac
     4141 gtctgaagtt ctaatcatct taattgcaat taatttagat aacacaaaac caaactcata
     4201 agcattacag ctcggtttct cagaatccat gtctacaatc agtgactgct ctacaaaatt
     4261 cacaattgta tgtctgtgat taaaaccaaa attaccaagc cattctatat actgatctgt
     4321 tgttgatatg ttccccatga taaaaccacg ataacatgca cgcaaaagct gtggttcaag
     4381 tttcattttg ttgccaaata atttagcctg tatagctgaa tttattccag cattaacact
     4441 tttttcacta cctgatatta tctcattaat aattttagtt aataattcga cttgttcagc
     4501 agtaagatct ttttcattca aaagttccct taatgctaag acttgatcac ttggatcttt
     4561 aaaatatctc tttagtctgt ctataaaatc ccgatctaga ggaaggttat cttttaacgt
     4621 tctctttaaa tcaaaaatct tgtgattaat ttcatcttct tccccatcta aaatacgttc
     4681 ctgactatca ctatttgttc ctttcagttt ctcaaggtct cttctgttta taaatgacga
     4741 aagagcggaa gccatctcat cagatgagtc aatatacttg ctattagcca agccagaatc
     4801 aagctctgca tcagcagtct catctccatc atctgaattt gtcattgcat tcaacttatc
     4861 aatgaatgca gagctaaaaa ctcctgtatt tttaacatca agcatacatc acctacttta
     4921 tagttagtaa tttaagtatg aaaccaatga tttttcgtct tccagcaaag tagtttcaga
     4981 tgggattatt tgtgtagtct tttgtatttc tctttcagat atgaggcttt tatattctgc
     5041 agtattataa taacttgact cctttatctc acggggttga atcagaaaaa cccttacaat
     5101 attactgata ttacttgttt tatacttgaa cacattaccg attacaggaa tagaggagag
     5161 aaaaggaata gatattattt cgttagagtt cgtttcatgc gtataaccac ctatcaacaa
     5221 acttttgcct tgtggaactc tcgctattgt actgattttg gtccttccaa cttctggaag
     5281 gacagatgta ttttcattat tataattgta atttgactga ctatttcctg ttccatcctc
     5341 tatcgttaaa gacatctcaa tttgtccccg agatgaaaag cgaggtataa catttatcaa
     5401 tgttccatac gtaacatgct ccaacgaaga attgcgctca ccaactaatg acacataaaa
     5461 agtcctgtta ttatcaaata tagccggaat attctcttgt gtcaaaatca ccggccttga
     5521 aactacattt gctttctttt tctgatttaa tgccataact gaagcaataa acttattgcc
     5581 gtccaaagtg cttatcgatg ctgatgagct catattaaat gacgcaccaa acgaatcacc
     5641 aaatgaagcc gttccttgcc aattaacacc aaggttattt aattcagatt tatcaatatc
     5701 gattatccat aatgataatt ctatatgtcg tttggctata tctaactgag tgataatatc
     5761 gcgaataatc tgtatttgct gatcattacc tttgactaat atagaattgg tttctggata
     5821 tgcaataagt gatacatcct ctaatataga actattggta actgaactaa atgaaaagtc
     5881 attactatcc tcagatactt tctgtgtaat attaaagggt ggcatcggat cattttgggc
     5941 ttgtctgtta ctcagagcct taccattatt taataaacgt tctacaacag tagccacccc
     6001 tgggatgaca atatcctccc ctctcatatt ataagttctg tcactaacaa atgtattttt
     6061 taattttata accccaaaat taactttatc tgtcccaata gaagaaacct gcttatcaag
     6121 caaagttgct gtattagcta ccagttcaac caatgccgga ggaccagata tatagaatgt
     6181 tttatcacta atgtttcctc ttataggata tctgtgatca taaaggttag catccttcag
     6241 atactgaata agataattaa gtgatatatt ctccagcaaa ataactttgc ttattaattc
     6301 tccagagtca tatatgtata atgcatttcc atctttatac catatgagtc ccacaagtaa
     6361 tgtcagtttt tcaagcattt cttctggatt agataaatca aactccccag atattctctt
     6421 ttttgcagct tgtttgctaa caacaatagg ataatttaat aacgcagaaa aacgttcaaa
     6481 aaaagatcct accgtatcac tttgagcaac atatttcgct atgtcatttt gttctaataa
     6541 atgagagtca atattattag cattaactat caatggtaac aatacaatca agagagtcaa
     6601 agatttaata ttaaattttt tcatttattt ttttcactaa aaaagtaata ttcgataatt
     6661 ctcttgcaga aaagcctagt cgtgttttta tctcatttga aaaatgggat gttgatgcat
     6721 acccattatt catggcagct gaagtgattg tttgattgtg caaaaataca tctaataaac
     6781 catttactaa ccgccaagtg ttcagttgtt ctttgacttt tgcccctaaa gcttttctac
     6841 ataaagatct aaaataagcc tccgacacac cataatgctc tgtcagactc tttattctta
     6901 cctccttttc tttctcaata gttctcagaa gataaagaac caaatgatat gactcagtca
     6961 ttcgtatcat agccagtgca ttacttacat tctggtcaga tgaaaagatg aatcttaaca
     7021 ttaacttatc aagcatcgat tcggtattac aactatcccg aagatcattc tcatgataga
     7081 aatgaataca tttttcagca tggctatgct ctactagagc accactgaaa tagtcccaaa
     7141 aagcaagaaa ttttgcacaa ctattaatgt taaagcgata atgatagttc acattatcac
     7201 tgaattttat attttgattt cggacaaaaa aaaacccttc catgctggag ccagctcaat
     7261 tatgttacgt tctttatctt ttacaagctc aacgttaact ggttcatgtc cactagagta
     7321 aatgtaaaac atattacttg tatttagacc tttatattta ctcacaatac aattatatcc
     7381 ctcgaattaa ttttaattta acttccccat ccttctctat aaggatggta tcatttatct
     7441 tattaccttt taaaacaacc ccgtctttca gcatagaaaa tagtttatct atagctatct
     7501 tttgtgcatc catgaagcac ccatctactt ccttaacatt aaagttgccc atgaatttac
     7561 ctgcattaaa actataaacc gccttaccac taatacattt agagtaaaca tttatacttt
     7621 tattggttgt attaaacgac cataataaat catttggaat agaaaaataa tccttactta
     7681 caggaacaat atgccattct ttttcagaat tagatgatga ctttaaagca cacccactca
     7741 gtgttaatag caatatactt aaaataaata ttttcaactt attactacca tgtcgaatca
     7801 tctgcctctc tccctgcttc aaagacacta attaatgaat cgaaggttgt agtcttaata
     7861 tagaaatcaa tattgttata tatggacaat gatgcataaa atccctcatg caaatcgaca
     7921 tattgatttg catctatgag tttattaaga agtgaacttt tttgtgcagc tgtaagataa
     7981 ggaaaacgtg aattgaataa ttgttcaatc ttattgtgca gttcatcatt atctatggaa
     8041 tttaattcac tctgtgagaa atattgcatt atttcataga ttatatcctc atcaaatgat
     8101 tgcgaagatg ataatctctc atttaaatta acaacttctt cactatttaa tctttgctgc
     8161 agagcattgc ttgcatttat ttgattaatc attaacagga ttctctttta acaagtcttc
     8221 tgcaatttgt ctcagtttct tatgtgctaa ctctttgaaa tattttttat cattttttgt
     8281 taatttttcc gcaagttcta ttttaacacc tgatattaca tcctgaggac taaactccac
     8341 tacttgatta ccactacaga atatataatt attcccttca gaatatttta aaataatctt
     8401 gacatctgag cgatatttat ggagatcaag ttctaacttt ttcctcagtg ctaaattaca
     8461 cttgggcaaa accactttta attctgttac tgtattacgc aacgaactaa gccactgctc
     8521 caataaagtg ctaatgatcc tctcatcatg taaattttct gaaagcaaac tagttatata
     8581 gtcctcaata tttcttctat tttcattttg ctttaacttc cattcacaaa tatagtcaat
     8641 aatatgctca aaagcagtct gtataccaat ttgataacca tcacatacag catctatacg
     8701 cagtgattca gcttttttcg tagcctcctt tataacacaa attgctttct tcttactctc
     8761 agtgtctcta tcttttatgg ttttacataa ttccttttca attctcttta taacaacacc
     8821 atcataggca tgatgtctgg ataccggtaa agtgcctttt tgcatattgc ataccttcat
     8881 ttaatcggga caaatttctt tctacaggcg tataatttcc ctccaaagca attggattaa
     8941 ataataagtt tattctttca tatataccaa cagatatatt ttccttccac gcaataagat
     9001 gccaaaaacc acttcgaata gcggcctccc cttgcttact gattttgccc ccataataac
     9061 taataaccat ctcactatga ttaattaacc gagactcttg actaccgata aaataagcaa
     9121 cagctggaag taaattccag ttggcaacaa acatagcggt taccccgctt aaaggttcaa
     9181 tatcgcaatt tagatcatat ttattaatta tgagattatt aatctcactt ctgattacgc
     9241 cattttctaa cagttcagaa ggcaaattca accgatttct atttatatag aacgctggat
     9301 caaaaattat tgaaagatat tttttataaa ttccatccat tcttatcata ttttgtttct
     9361 cttgaaccac cctgttttga atgcccatac caaaagaatg acaactagta cagccatccc
     9421 gagaaataaa tatattactt catttgttaa aaattccgat ttaacttcct taacaggttg
     9481 tacatttgta taaacatatt cttctttcgg agttaatatg acagatatat tttcatactt
     9541 aacatcagaa aaggtgtttt tcaaaaatcg cttaatatta ctaactaata gttcagactc
     9601 tttcggtgag tcatatatag cgattactga tatatgcatc ggttttgaag atatattttt
     9661 ttcttcaaga tcatagctaa catgtatttt tgccgaaata acaccaccaa tagaaactaa
     9721 agactgttcc agccgttgct caatagcact atataaacgg gccttttcag ctcttggaga
     9781 agacactaat gaatctgtag gaaacatttg tgagatatca actctctccg gatttggcaa
     9841 atcgtacatg cgcatcaaat caactgccga tgcaaatgtc cccttttcga cttgtaccga
     9901 gatcccctgt ttacctccat caacttttct agcagtaata ttatggcgtt ctagcacaga
     9961 tattatttca tttgcctgtc tttgagataa attagaaatt aactcttcac gttgctcaca
    10021 tccaatcaac atcagcaaca agaataaaat aaaaccttta tacctaatca tataaattaa
    10081 gactttaata aagtttcaac agccgaaact gtttttcttg ctaacgtgcc agctaatgat
    10141 actcctattg aataatttga gagcgtcgtc tgtaacttgg ccaaagattc tgggtttaat
    10201 gattctggat tgcttaccat ctccattatt tgtgatactt gaatatctgt atccatctta
    10261 atatcactat atttagccga cacgacgtct tccagacttg atatctcttg agattgaaaa
    10321 tcactggctt tgataatatc aacctgattg actggataaa tgtaattcat gaatgctccc
    10381 tattatctga agttttgaat aattgcagca tcaacatcct taatcacttt cactgtattg
    10441 gattgcgcgt tcctatataa tgtatattca gataatttac tttggtattc agccagcaac
    10501 tgtggattcg aaggattttt agctaattta tctagtgcca atgttagttc accttgtaat
    10561 gtttgagttc catcatcaaa agtttcagat aatgaactca atgtccaatc atcattcggt
    10621 actgtaacac tcattttatc ctcacttatt tttatctaaa aaaaaccagt gtttatcatt
    10681 caacattaca taactgtctt tgctgttaag atatgattta cctttaaaat catcatctat
    10741 caaaagtaca gaaaactcaa tatactgatc tccatatata tttttatgtt cagaaatcag
    10801 tctattaatg agctcaagct tttcatctgt cagttcttct ctgacagaat atgtaacttt
    10861 attattttca caaatctctt tatactggac atttaccttt gtgaaaatac ctttagcatc
    10921 cccccttgca ttatgatcgc taagatagtt gaatttaata tttttgatat acggaaattc
    10981 aacaatcaga gcatttttca atttatcaag ttcggtatct tttgaactat ttctttcttt
    11041 acttaaaaac accagtaatt ccgctttatc actcactaaa ttcaacacat acaaattgat
    11101 aaatgggaat cgttgattga tatattgttg aattctattt atttctttgt ttgacaccag
    11161 aatatatctg catttggtaa atccggtttt atttaaggct acagacgccc ataccgcatc
    11221 attttgtgtt tcggcaagaa tataattgca ctgtccagtg ttgactattc catatctctt
    11281 atcatctata atttcagcaa tttcttttac ttcatctttc tttaaagaga ggaaaaaaat
    11341 tataattaac acaacaataa cagcaaagaa aaaaaacacc gagtggttct tatacattcc
    11401 gtttagtata tggcctctcg atttatcctc cctcatattt ttaaggtgaa aagaaatccc
    11461 gtcatattca aatgaattaa attgtgaatt gatatcaatt tgctcacctt tttcatcact
    11521 aaattgacag atattatctt tgcttatggt caaataaatt ataccatccg atttactatc
    11581 tgttacagga ataatatttt ctgggaaatt gtcgtttcca aactcaagtg tttctatcgt
    11641 ccgtccgaga acaattaagt tattcccata atataacgga aactcatccc cgacaccgtt
    11701 tgtgagtttc actaataacc tgaatggtgc aagatttgag ttctttgcct cagacataaa
    11761 tcctcttata tccttcgatt attctgcttg ctctctgctg ctatccgcaa atagcgtcta
    11821 tagatatcct ctgcgtactt taatctttct tttttctttt tcggagacgt tcctgcatta
    11881 taggctccaa cagcctccca ccctcttcca tacatcatca taaactcatt taataacttt
    11941 gctgcataat gtacagaaag acatggatga ctaattagca tttcctcaga atatcccatt
    12001 tctctaagtc ttttagaatg aaagtcattt atttgcatta taccataatc tttacttcca
    12061 ttgttattaa cattgactgc agatttatta aatccggatt ctttttccgc aatcgctttt
    12121 aacaaacttg atggaatatt gtacctttca ccagccttat cccaacaatc agctctccct
    12181 aaatatggga taaaacacag caaaataaat acgaaacggg acattaatac cccttcattc
    12241 ttcgcgcaaa ttcatccttc agccaacgca cacgcgaaat ataatattct gtattaacat
    12301 atacatcctc gaattcattt atcccggtaa gatctaatcg cgcaattaga tttccgcctt
    12361 catcatcagt agctaagcat atcttttctg agtaatttag gctcaacgca tatataagat
    12421 catttatatt ttcaggcaat gcacaaaaag ggcataataa atttattgaa tctccttctt
    12481 tttcaatata tattagcaca tcatcatcaa gcatgattgc ttgatcatcg atatcaatta
    12541 aagggatacc caaggctcgg caaataacat ctgctaaatc ttccatatat tcctcttata
    12601 caaatgacga ataccctttc accatattcc atatttttgg gtcccctatt ctttcagaat
    12661 aagatagctc taacgaagat agcggcaatt ttttcatgac tttatttccg ggcacaccag
    12721 tattcatctc ttggatttcc atattaccac tattcattag aatagtagaa aataatcttt
    12781 tctcttctga ggataattta ctatttaatt gagaaaactg acctgtttca tgttttctta
    12841 ttatttctct tttaatttca gcatcctgca tacctgttct gtccttccca ctcttacaat
    12901 tccagcaagg tacagcacct atagtataag ccaatagagt cattctttgt gacaatttat
    12961 acggctctcc attatcattt ttttgtaact ttttggttac aatctctttt atctggttag
    13021 ccagatatat cacatcattc ttacatggtg gatttttttc tattgcttct gcagcccaac
    13081 ctccaatcac accatttttg aggaaattat cccccaacaa agaacatatt gattcatcat
    13141 taagcttgtc aacattcctc cagcctaagc ccatttttag cgctaattca tttacaccaa
    13201 agttaaatgt caccactttt agattaacgc ttacttcttt cagaagacca tcactattcc
    13261 gaattaataa tttcgttggc ttcccccttt tagagtttaa cccttttaat gcgttcacct
    13321 gatcttttaa catactctcc tctcccccgg ttaaactggt cggcgtcaga agagaagtcg
    13381 aaacaatctt taaatctact gttttaccag acaaagcctg tgataataac tctggtctgc
    13441 tatataatgc tgcgcttact aactcctcag ctttattacg agcagcaaca gctctttcag
    13501 atgagttttt tttcaatcca taggctgata taactccatg tctgataccc gaaaaaattt
    13561 ccttcccctc atcatccacc acttttgaaa gccacatatt cgcgatatga tcactctctc
    13621 tcgtagaagc acaacaaatt ccttttccat tatactcttt gacaaatata tttttattcc
    13681 ctatcttcat atgagaggca ggagttaatt gaaaaccata atttttccca tgatgactaa
    13741 tattcttgtc gaccggtccc caattcttat tatttaaata atcagtaaat atttgtttta
    13801 ttttcgtttg caattccttt ttatttttaa tgcctagctg tgctcttatc tcttcactta
    13861 ttactttatt aagctcatta atatgtcttt tattaacttc accaagttta taggtatcta
    13921 atcgttccat catggcttta atttttgacg tatcaccacc tgccaacttt attgcctcgc
    13981 aaaaaacttc agctctgtca cgcgcaaaca aaaggctttt tcctgttaag cttgtttggt
    14041 tctgtaaata taatcgatta agcgcttcga tccccttatt tctttctcca tttttaactc
    14101 tttgatatga aatcacgctt acacctttgt gctttccggt ttcttctgcg cctttatagg
    14161 aatctccgct ttgaaatgaa acctgatgca atcccaaatt agttatgtgc atttaattat
    14221 cctcagtcat aaatggggca ttgattgcag ttttgtacct cgttttacaa caaaaaagat
    14281 acttattcaa cagggttctc tctttttcta taacactccg agcctgatac atcaggcact
    14341 ttatcttgtg ggatttcatg atgaaacgag cactacatat gctcacgagg tacatattat
    14401 gtacagcaaa agaaaatttg attatgttaa caggctcaag tttctaaatt tattaattga
    14461 atatattata taccaaatac attttacatc cccacaatca ccaagtaatg gagagttaat
    14521 aaagtatgat cctcaaaatt agcaatttca ttgacgcaag caatacaaaa gggcctatac
    14581 gcgttgaaga tacagagcat ggacctatat tgatagcaca gaaattcaac ctaaaggatc
    14641 tctttttcag aacattaagc actattaatg caaagataaa ttcacagata ttaaatgaac
    14701 aactaaaaaa ttatcgacta gagaatcaaa aatccttatt gcttttcctc aatacattag
    14761 ctagcgaaaa atctgcagaa agtgcgtttg ccgcctatga agcagctaaa aactcaattc
    14821 aacactcttt cacaggtaga gacatcaaat taatgttgaa taccgcagag cgttttcatg
    14881 gcatcggtac agccaaaaat cttgaaagac atttagtttt tcgttgctgg ggaaatagag
    14941 gcataaccca tttagggcat actagtatca gtataaaaaa caatttacta caggaaccaa
    15001 ctcataccta tctcagctgg tatcccggcg ggaatgttac aaaagataca gaaataaact
    15061 acctttttga aaaacgctca ggttacagtg tcgataccta taagcaagac aaactaaata
    15121 tgatttcaga ccaaacagcc gaaagacttg atgcaggcca agaggttaga aacttattaa
    15181 attcaaaaca agatcaaaat aacaacaaaa aaatattttt tcccagagct aatcaaaaaa
    15241 aagaccccta tggttattgg ggggtcagtg ctgataaagt atacattcca ttatcaggtg
    15301 ataataaaac aaaggatggt aaaatcagcc ataacctttt tggccttgat gaaacaaaca
    15361 tgtcaaaatt tatatgcaaa aagaaagcag atgctttcag acaattggca aattataaat
    15421 taatcagtaa atctgaaaac tgtgctggta tggcacttaa tgttcttaaa gcaggaaatt
    15481 ctgaaatata ctttccactc cctgacgtta aattggttgc tacacctaac gatgtatacg
    15541 cgtatgcaaa taaagttagg caacgcattg aaagtctcaa tcagtcttat aacgaaataa
    15601 tgaagtatat tgaatctgat tttgatcttt ccagattgac tcaattacga cgcagctatc
    15661 ttaaaagttt caataaaatt aaccttatcc acacaccaaa aacattcaag cctttatcaa
    15721 tttcacttta caaacatcct actgaaaatg tatcttcaga agactttgat gcggtcatca
    15781 acgcttgcca ctcatactta gtaaaatcag cacccagcaa tatgactcgt gtattaaacg
    15841 aattaaaaac cgaagcaaca gataaaaaag aagaaatcat cgaaaaatca ataaaaatta
    15901 ttgattatta caattctctt aaatcgccag atcttggaac caaactttac attcacgact
    15961 tattacagat caataagctt ttattgaata actctcattc taatatatag aaggccatag
    16021 aaatgtgtcg caaactatat gataaacttt atgaaataac aggtgcaaag cttgatttta
    16081 acgataaaaa tcaagccttt atattgctcg aagaacaaat acctgtttgt ataacagata
    16141 atgatgaata catctttttg accggacttc ttaatgaaca tgaacttttt acagaaaata
    16201 ttattaatcc agaacacata ctcatcctta actactcttt gagtagagat tatggttctt
    16261 ctatctgtct tctccctgat actcatcaat gtgttttgac caagaaacac tacaaaaaat
    16321 atctttcacc tgatgaactc attgaatctt tgtatgaatt tttattttgt attaaactaa
    16381 ccattgcaaa catcacttca gaagtgaact aacatatagg gggtatcatg caaattctaa
    16441 acaaaatact tccacaggtt gaattcgcga ttcctcgccc atcatttgat tcgttaagtc
    16501 gcaataagct tgtaaagaaa atattatctg tttttaactt aaaacaacgc tttcctcaga
    16561 agaattttgg atgcccggtg aatataaata aaatcaggga cagcgtcata gacaaaataa
    16621 aagatagtaa ttcggggaat caactatttt gttggatgag ccaagagcga accacttatg
    16681 tctcttcaat gataaacaga agtatagatg aaatggccat acacaatgga gttgttttga
    16741 cctcagataa caaaaaaaac atatttgcag ctattgaaaa aaaattcccg gatataaaac
    16801 ttgatgaaaa atcagcacaa acatctatca gtcatacagc actaaacgag attgcctcat
    16861 ctggcctcag agcaaagatt ttaaaacgct atagcagtga catggatttg tttaacacac
    16921 aaatgaagga tcttacaaat ctagtatcca gttccgtcta cgataaaata tttaatgaat
    16981 caacaaaagt tttacaaata gaaataagcg cagaggttct aaaggctgta taccgtcaaa
    17041 gcaatacaaa ttaaatcttt tatattaaat cttatactta ttaaagtaaa gcagacaaaa
    17101 aggagacctt atgtctttaa atatcaccga aaatgaaagc atctctactg cagtaattga
    17161 tgcaattaac tctggcgcta cactgaaaga tattaatgca attcctgatg atatgatgga
    17221 tgacatttat tcatatgctt atgactttta caacaaagga agaatagagg aagctgaagt
    17281 tttcttcagg tttttatgta tatacgactt ttacaatgta gactacatta tgggactcgc
    17341 agctatttat cagataaaag aacagttcca acaagcagca gacctttatg ctgtcgcttt
    17401 tgcattagga aaaaatgact atacaccagt attccatact ggacaatgcc agcttcggtt
    17461 gaaagccccc ttaaaagcta aagagtgctt cgaactcgta attcaacaca gcaatgatga
    17521 aaaattaaaa ataaaagcac aatcatactt ggacgcaatt caggatatca aggagtaatt
    17581 attatgcata atgtaagcac cacaaccact ggttttcctc ttgccaaaat attggcttcc
    17641 actgagcttg gagacaatac tatccaagct gcaaatgatg cagctaacaa attattttct
    17701 cttacaattg ctgatcttac tgctaaccaa aatattaata caactaatgc acactcaact
    17761 tcaaatatat taatccctga acttaaagca ccaaagtcat taaatgcaag ttcccaacta
    17821 acgcttttaa ttggaaacct tattcaaata ctcggtgaaa aatctttaac tgcattaaca
    17881 aataaaatta ctgcttggaa gtcccagcaa caggcaagac agcaaaaaaa cctagaattc
    17941 tccgataaaa ttaacactct tctatctgaa actgaaggac taaccagaga ctatgaaaaa
    18001 caaattaata aactaaaaaa cgcagattct aaaataaaag acctagaaaa taaaattaac
    18061 caaattcaaa caagattatc cgaactcgac ccagagtcac cagaaaagaa aaaattaagc
    18121 cgggaagaaa tacaactcac tatcaaaaaa gacgcagcag ttaaagacag gacattgatt
    18181 gagcagaaaa ccctgtcaat tcatagcaaa cttacagata aatcaatgca actcgaaaaa
    18241 gaaatagact ctttttctgc attttcaaac acagcatctg ctgaacagct atcaacccag
    18301 cagaaatcat taaccggact tgccagtgtt actcaattga tggcaacctt tattcaacta
    18361 gttggaaaaa ataatgaaga atctttaaaa aatgatctgg ctctattcca gtctctccaa
    18421 gaatcaagaa aaactgaaat ggagagaaaa tctgatgagt atgctgctga agtacgtaaa
    18481 gcagaagaac tcaacagagt aatgggttgt gttgggaaaa tacttggggc acttttaact
    18541 atcgttagtg ttgttgcagc agctttttct ggaggagcct ctctagcact ggcagctgtt
    18601 ggtttagctc ttatggttac ggatgctata gtacaagcag cgaccggcaa ttccttcatg
    18661 gaacaagccc tgaatccgat catgaaagca gtcattgaac ccttaatcaa actcctttca
    18721 gatgcattta caaaaatgct cgaaggcttg ggcgtcgact cgaaaaaagc caaaatgatt
    18781 ggctctattc tgggggcaat cgcaggcgct cttgtcctag ttgcagcagt cgttctcgta
    18841 gccactgttg gtaaacaggc agcagcaaaa cttgcagaaa atattggcaa aataataggt
    18901 aaaaccctca cagaccttat accaaagttt ctcaagaatt tttcttctca actggacgat
    18961 ttaatcacta atgctgttgc cagattaaat aaatttcttg gtgcagcggg tgatgaagta
    19021 atatccaaac aaattatttc cacccattta aaccaagcag ttttattagg agaaagtgtt
    19081 aactctgcca cacaagcggg aggaagtgtc gcttctgctg ttttccagaa cagcgcgtcg
    19141 acaaatctag cagacctgac attatcgaaa tatcaagttg aacaactgtc aaaatatatc
    19201 agtgaagcaa tagaaaaatt cggccaattg caggaagtaa ttgcagatct attagcctca
    19261 atgtccaact ctcaggctaa tagaactgat gttgcaaaag caattttgca acaaactact
    19321 gcttgataca aataaggaga atgttatgga aattcaaaac acaaaaccaa cccagatttt
    19381 atatacagat atatccacaa aacaaactca aagttcttcc gaaacacaaa aatcacaaaa
    19441 ttatcagcag attgcagcgc atattccact taatgtcggt aaaaatcccg tattaacaac
    19501 cacattaaat gatgatcaac ttttaaagtt atcagagcag gttcagcatg attcagaaat
    19561 cattgctcgc cttactgaca aaaagatgaa agatctttca gagatgagtc acacccttac
    19621 tccagagaac actctggata tttccagtct ttcttctaat gctgtttctt taattattag
    19681 tgtagccgtt ctactttctg ctctccgcac tgcagaaact aaattgggct ctcaattgtc
    19741 attgattgcg ttcgatgcta caaaatcagc tgcagagaac attgttcggc aaggcctggc
    19801 agccctatca tcaagcatta ctggagcagt cacacaagta ggtataacgg gtatcggtgc
    19861 caaaaaaacg cattcaggga ttagcgacca aaaaggagcc ttaagaaaga accttgccac
    19921 tgctcaatct cttgaaaaag agcttgcagg ttctaaatta gggttaaata aacaaataga
    19981 tacaaatatc acctcaccac aaactaactc tagcacaaaa tttttaggta aaaataaact
    20041 ggcgccagat aatatatccc tgtcaactga acataaaact tctcttagtt ctcccgatat
    20101 ttctttgcag gataaaattg acacccagag aagaacttac gagctcaata ccctttctgc
    20161 gcagcaaaaa caaaacattg gccgtgcaac aatggaaaca tcagccgttg ctggtaatat
    20221 atccacatca ggagggcgtt atgcatctgc tcttgaagaa gaagaacaac taatcagtca
    20281 ggccagcagt aaacaagcag aggaagcatc ccaagtatct aaagaagcat cccaagcgac
    20341 aaatcaatta atacaaaaat tattgaatat aattgacagc atcaaccaat caaagaattc
    20401 gacagccagt cagattgctg gtaacattcg agcttaatat atccaagagc cataataata
    20461 tatggctctt cctgtaagga aataaccatg aatataacaa ctctgactaa tagtatttcc
    20521 acctcatcat tcagtccaaa caataccaac ggttcatcaa ccgaaacagt taattctgat
    20581 ataaaaacaa cgaccagttc tcatcctgta agttccctta ctatgctcaa cgacaccctt
    20641 cataatatca gaacaacaaa tcaggcatta aagaaagagc tttcacaaaa aacgttgact
    20701 aaaacatcgc tagaagaaat agcattacat tcatctcaga ttagcatgga tgtaaataaa
    20761 tccgctcaac tattagatat tctttccagg cacgaatatc caattaataa agacgcaaga
    20821 gaattattac attcagctcc gaaagaagcc gagcttgatg gagatcaaat gatatctcat
    20881 agagaactgt gggctaaaat tgcaaactcc atcaatgata ttaatgaaca gtatctgaaa
    20941 gtatatgaac atgccgttag ttcatatact caaatgtatc aagattttag cgctgttctt
    21001 tccagtcttg ccggctggat ctctcccgga ggtaacgacg gaaactccgt gaaattacaa
    21061 gtcaactcgc ttaaaaaggc attggaagaa ctcaaggaaa aatataaaga taaaccgcta
    21121 tatccagcaa ataatactgt tagtcaggaa caagcaaata aatggcttac agaattaggt
    21181 ggaacaatcg gcaaggtatc tcaaaaaaac gggggatatg ttgtcagtat aaacatgacc
    21241 ccaatagaca atatgttaaa aagcttagat aatctaggtg gaaatggcga ggttgtgcta
    21301 gataatgcaa aatatcaggc atggaatgcc ggattctctg ccgaagatga aacaatgaaa
    21361 aataatcttc aaactttagt tcaaaaatac agtaatgcca atagtatttt tgataattta
    21421 gtaaaggttt tgagtagtac aataagctca tgtacagata cagataaact ttttctccat
    21481 ttctgaggtg cgccatgcat aatgtaaata atactcaagc gccaacattc ttatataagg
    21541 caacttcacc atcatcaaca gaatacagcg agttaaaaag caaaatatcc gatatccata
    21601 gttcgcaaac ttctctaaaa acaccagcat cagtgtctga aaaagaaaac tttgcaacgt
    21661 cttttaatca gaaatgtctt gattttttat tttcttcctc agggaaagaa gatgtgttaa
    21721 gaagcattta ttccaactca atgaatgcgt atgccaaaag cgagattctc gaattttcaa
    21781 atgttttgta ctccttagta catcaaaatg gtcttaattt tgaaaacgaa aagggacttc
    21841 aaaaaattgt cgcacagtat tcggaactaa ttataaaaga taaattatcc caagattctg
    21901 cctttggacc atggtcggca aagaataaga aactccatca attacgacaa aacattgagc
    21961 acagacttgc actattagca caacaacaca catctggtga agctttatca ttgggacaaa
    22021 aactcctcaa tactgaagta tcatcattta tcaagaataa tattcttgct gaattaaagt
    22081 taagtaatga aactgtttca tctctcaaac tagatgattt agttgacgca caggcaaaac
    22141 ttgcctttga tagtttgcgc aatcaacgta aaaatactat tgatagtaaa ggatttggta
    22201 taggtaaact gtcaagagac ttaaatacag tagccgtgtt tcctgagctg ttgagaaaag
    22261 tccttaatga tattttagaa gatataaaag attcgcatcc tatccaagat ggcctcccta
    22321 cacctcccga agatatgcca gatggcggac caacccccgg agccaatgag aaaacatccc
    22381 aacctgtaat tcactatcat ataaataatg ataatagaac ttacgataat agagtttttg
    22441 acaacagagt atatgacaat agctatcacg agaacccaga aaatgatgca cagtctccta
    22501 cttctcagac aaacgatcta ttatcccgta acggaaactc attactaaat ccacaaagag
    22561 cactagttca aaaagtaact tccgttctac cacactctat atcagatact gtccagacat
    22621 ttgcaaataa ttcagcttta gaaaaggctt tcaaccatac tccagataat tcggatggaa
    22681 taggttcaga cctgttaact acgagtagtc aagaaagatc tgcaaataac tctctttctc
    22741 ggggacacag gcctctgaac atacagaact cttcaaccac cccccctctc cacccggaag
    22801 gagtgacaag cagtaatgat aactcatcag atacaactaa aagtagcgct tctctttctc
    22861 atagagtagc ttcgcaaatc aataaattca actcaaacac tgattcaaaa gtacttcaga
    22921 ctgatttttt atcaagaaat ggagacacat atttaacacg ggaaacgata tttgaagctt
    22981 caaaaaaagt aacaaactcc ctaagtaatc ttatatctct cattggaact aaatcaggaa
    23041 cacaagaacg agagttacag gaaaaatcaa aggacattac aaaatccaca acagaacata
    23101 gaataaacaa caaattaaaa gttacagatg caaatataag aaactacgta acagaaacca
    23161 acgcagatac aattgataaa aatcatgcga tctatgaaaa ggcaaaagaa gtatctagcg
    23221 ccctcagcaa ggtattgtca aaaattgacg atacctctgc agaattactt acagatgata
    23281 tatctgattt aaaaaataac aatgatatta cagctgaaaa caataatata tataaagcag
    23341 caaaagatgt aaccacttcc ctatcaaaag tattaaagaa tatcaataag gattaaaata
    23401 tgataaaaga aaaaatatta tcaatagtgg ccttttgcta tggtatagcg tacagcaaac
    23461 tatctgaaga aactaaattt attgaagatc tttctgccga ttctctttct ctgattgaaa
    23521 tgctggatat gattagtttt gagttcaatc tacgtataga tgaatctaca ttagaacaca
    23581 ttattactat tggagatctt atttctgtag tcaaaaatag tacaaaatca atatagtaat
    23641 tgataagcat tttttcatct atggagctct cacatcagag ctccacaaga atattattct
    23701 tttatccaat aaagataaat tgcatcaatc cagctattaa aatagtaatt tttaagacta
    23761 ccgttgacta tcatcaacag ttacatacaa tcttgctcac actgcattta acttttgtca
    23821 atataaatca atatgaataa acagggtgtg atatggtgga tttgtgcaac gacttgttaa
    23881 gtataaagga aggccaaaag aaagagttta cactccattc tggtaataaa gtttccttta
    23941 tcaaagccaa gattcctcat aaaaggatcc aagatttaac cttcgtcaac caaaaaacga
    24001 atgtacgcga tcaagaatcc ctaacagaag aatcattagc cgatatcata aaaactataa
    24061 agctacaaca attcttccct gtaataggaa gggagattga tggtagaatt gaaattctgg
    24121 atggcactcg tagaagagca tctgcaatat atgcaggagc agatcttgaa gttctatatt
    24181 caaaagaata tatatctact cttgatgcca gaaaactagc aaacgatata caaacagcaa
    24241 aagagcatag catccgagaa cttggtattg gtcttaattt tctgaaagta tcagggatgt
    24301 cctataaaga catagccaaa aaagagaatc tgtctcgcgc gaaagtcact cgtgcctttc
    24361 aggcagcaag cgttccacag gaaataatat ctctatttcc aatcgcgtca gaacttaact
    24421 ttaatgacta caagatatta ttcaattatt ataaaggact tgaaaaggct aatgaatctc
    24481 ttagttctac actaccaata ttaaaggaag aaataaaaga ccttgataca aatttgcccc
    24541 cggacatata taaaaaagaa attttaaaca tcataaagaa aagcaaaaac agaaagcaaa
    24601 acccttcgct aaaagttgac tcgttattta tttctaaaga caaacggact tacataaaaa
    24661 gaaaagagaa taaaacaaac agaactctaa tatttacatt atctaaaata aacaaaacag
    24721 ttcagagaga aatagatgaa gctattcggg atataatttc tcgccatcta tcgtcttcat
    24781 aaacatcgaa cactgatgta aactgcccaa ttcagataat aaaaatcttc gttgaacttg
    24841 actttcagcc ggtggggtaa ttccgtcggc tatctgttat tcaatcccca cgcactgtgt
    24901 ttccttaaaa ttacggtgaa aactcatacc ctgctgacca cgagaaaatt atctggtgaa
    24961 attagtaata ttcatatcat tgaaaatgaa ttgtgcaact aagggatgcg gcatgatggt
    25021 caaaatttta tgtgaatatg agtgactggc cgaaaatatt cagcgtattt ttaatcgttt
    25081 caataaaaac agcgaggatt agcagtcaac gcttttacta ctcaattttt tttacatggt
    25141 gataaacaaa aaatccacca ttaacagact tcgttcacag tatcaaaaaa gctttaagaa
    25201 tatattagca gatttctcaa atataattac tgttttattc tcctaatctt cccataaaat
    25261 gaccgactta cattatttta taattataaa caagtttgat tttaaaatca tattaattat
    25321 taacaagaga tttatttttc taaccccaaa agttatgctt ggtcaaagag taacttataa
    25381 tatccatacc cctcataggg cttacagttt aaattgcggg tataaaatac atctattttc
    25441 aaataaaata aagcatactc caattacaac gagtgactaa aataaaaata tcatccaagt
    25501 caatcactgt ttgctctgca aaatatgtca gccttctcaa acttaccagc acttaaatat
    25561 ttaagcacac aatcattcct tgagggagtt cacttatttc aagagaaaca tttcgtcttc
    25621 ccacacaact aatccgccca atatagctaa atttgtactc agtaatatca tttgttatgc
    25681 cccccacccc aaaatattca agctccttca cagataaaaa acgacaattt acaaagcctc
    25741 attagttata actatggaaa tacctatttt agtatatcct ataaagttag aattacttct
    25801 tcttgcacct aattccatag ttgaaaaaca agaataattt tctcctacag ctggatccat
    25861 atagcttcca tcaggtctac acaacaccca gtggaggccg acaggaactc caataaagga
    25921 tacagcaaca gcttccaaaa ctctttggtt agaagataac acatctcgtt gatgtactat
    25981 attacatccc atgccaatta ataagtccct tgcatcggga taaataaaac taattacttg
    26041 cgaaaaaatg ttactttctt caacgacata agcattcaat ccaagaagcc tcgtagccat
    26101 gactatctta tcaggcatag aataaccggc atccgcgata ttatctttgt gtattctcgg
    26161 attataatta ccactagtta ttaagtataa atcccgctca caacgattat ccaaatcaag
    26221 agaactcttc cttgtcattt cggacataga ccctttgtat tgtggtattt tatcaacccc
    26281 taattccttt gcagcacaga gtaaactggc agctccacaa gaattgtcat acatctgctt
    26341 cctcgccaaa aaacaacctt gttcgtttac tctattctca tttaccctca catcagtctg
    26401 atgagatatc atatattctc ttggtatagc cccctgcaat aaaaccccat ataacataac
    26461 acctgtatga atacatcccc gtttgcaagg cttccgttgt tccgacatcg acgagtaatt
    26521 gcagcacagt attgcatcca gcaatgcgaa ctactatgag aattagctga ccgggtcctt
    26581 attccccgaa cgaactttaa atccaaaacc tcatactctt ttccgtcgtc atcacgtccg
    26641 tcaccataac aacctttcag catccaccct tttattaaga gttaatgtcc atcagcatca
    26701 gcaattaata ccgcctgata ctgacatatt tttgtcgtat ttacgtccat aatcggttat
    26761 tgtgatacag gtcacaccag cttatttgct attgttacaa taaccacctt ggttgagagc
    26821 tgttttttca actttgttat caactccccc aactccttgg tcagtaaggc tgccgaccga
    26881 gcacagtgaa tagatagtat ccgcaggtac caccatgctt acttaatact cagaaggcga
    26941 agtgctctcc aattaactct gccgcactat gcttataagc tgcttttcaa tgccgtctat
    27001 caacatgtcc aattcattca atctgttgta ctggtaatcc aacgcagtaa tgatatatgc
    27061 aggtattctt tcatccactc cagtaacgtt tcatgcaaag cattaattta cgcgctacgg
    27121 aacttcacca gttgactgag caaacgatgt gggaccagta ctgcctgcag ttcttctatt
    27181 gttacagcga tttctttacc aaacagctga accaccatca agataaagat agcctgaaca
    27241 ttcatcgcac cattttgtta ccaatggcaa aagatttaac gaaccgtgcc tgcatcaggc
    27301 ggacttttta tccctgcttt gttagctccc taaccttgta cagagagcct ccataagctc
    27361 ccatatcaag cagacatggt tcgcagttac tgacatacac cagaaaactc tgacagcaga
    27421 ctcacttatc ctccacttca ctcatatcct tattggtgag atgaacctga atgacatgtt
    27481 ttgcgacata aacgccaacc agtgtatatt tcacctgtag agattctaca atctgtagcg
    27541 ttatgcatct aatgttaggc tctatgatgt cagaaatctg cgagactcta caccttctgc
    27601 atttaccttt atttatcacc atgctcctca gttaacggag aacgtgttca ttgcattttg
    27661 tcacttacat ttcgcctttg gtatcggggg gatataggtt ccaacgttcc gcatgagaac
    27721 ctgaaccggc ttcacgccac cttgatacca gatgccgcac aggtagtcaa caagtgtcac
    27781 gaacggtgca atagtgatcc acacccaacg cctgaaatca gatccagggg gtaatctgct
    27841 ctcctgattc aggagagctt atggtcactt ttgagacagt tatggaaatt aaaatcctgc
    27901 acaagcaggg aatgagtagc cggacgattg ccagagaact ggggctctcc cgcaataccg
    27961 ttaaacgtta tttgcaggca aaatctgagc cgccaaaata tacgccgcga cctgctgttg
    28021 cttcactcct ggatgaatac cgggattata ttcgtcaacg catcgccgat gctcatcctt
    28081 acaaaatccc ggcaacggta atcgctcgtg agatcagaga ccagggatat cgtggcggaa
    28141 tgaccattct cagggcattc att
//
LOCUS       DAJTUI010000052        27380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTUI010000052 DAJTUI010000000
VERSION     DAJTUI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.052"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            282..1580
                     /gene="kgtP"
                     /locus_tag="OO464_002339"
     CDS             282..1580
                     /gene="kgtP"
                     /locus_tag="OO464_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6227467.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1577..1900)
                     /locus_tag="OO464_002340"
     CDS             complement(1577..1900)
                     /locus_tag="OO464_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6227468.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1946..3301)
                     /gene="pssA"
                     /locus_tag="OO464_002341"
     CDS             complement(1946..3301)
                     /gene="pssA"
                     /locus_tag="OO464_002341"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6227469.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3415..6075)
                     /gene="pat"
                     /locus_tag="OO464_002342"
     CDS             complement(3415..6075)
                     /gene="pat"
                     /locus_tag="OO464_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6227470.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6107..6805)
                     /gene="tapT"
                     /locus_tag="OO464_002343"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6107..6805)
                     /gene="tapT"
                     /locus_tag="OO464_002343"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6227471.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6874..7293)
                     /gene="trxC"
                     /locus_tag="OO464_002344"
     CDS             complement(6874..7293)
                     /gene="trxC"
                     /locus_tag="OO464_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6227472.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7500..8537
                     /locus_tag="OO464_002345"
     CDS             7500..8537
                     /locus_tag="OO464_002345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6227473.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8585..9274)
                     /gene="ung"
                     /locus_tag="OO464_002346"
     CDS             complement(8585..9274)
                     /gene="ung"
                     /locus_tag="OO464_002346"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6227474.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9579..9962
                     /gene="grcA"
                     /locus_tag="OO464_002347"
     CDS             9579..9962
                     /gene="grcA"
                     /locus_tag="OO464_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6227475.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10018..10605)
                     /gene="eamB"
                     /locus_tag="OO464_002348"
     CDS             complement(10018..10605)
                     /gene="eamB"
                     /locus_tag="OO464_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6227476.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10708..11589
                     /locus_tag="OO464_002349"
     CDS             10708..11589
                     /locus_tag="OO464_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6227477.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11624..12958)
                     /gene="srmB"
                     /locus_tag="OO464_002350"
     CDS             complement(11624..12958)
                     /gene="srmB"
                     /locus_tag="OO464_002350"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6227478.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13090..13827
                     /gene="trmN"
                     /locus_tag="OO464_002351"
     CDS             13090..13827
                     /gene="trmN"
                     /locus_tag="OO464_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6227479.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13812..15434)
                     /gene="nadB"
                     /locus_tag="OO464_002352"
                     /pseudo
     CDS             complement(13812..15434)
                     /gene="nadB"
                     /locus_tag="OO464_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15690..15845
                     /gene="rseD"
                     /locus_tag="OO464_002353"
     CDS             15690..15845
                     /gene="rseD"
                     /locus_tag="OO464_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6227480.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15842..16417
                     /gene="rpoE"
                     /locus_tag="OO464_002354"
     CDS             15842..16417
                     /gene="rpoE"
                     /locus_tag="OO464_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6227481.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16450..17100
                     /gene="rseA"
                     /locus_tag="OO464_002355"
     CDS             16450..17100
                     /gene="rseA"
                     /locus_tag="OO464_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6227482.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17100..18056
                     /gene="rseB"
                     /locus_tag="OO464_002356"
     CDS             17100..18056
                     /gene="rseB"
                     /locus_tag="OO464_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6227483.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18053..18532
                     /gene="rseC"
                     /locus_tag="OO464_002357"
     CDS             18053..18532
                     /gene="rseC"
                     /locus_tag="OO464_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6227484.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18730..20529
                     /gene="lepA"
                     /locus_tag="OO464_002358"
     CDS             18730..20529
                     /gene="lepA"
                     /locus_tag="OO464_002358"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6227485.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20545..21519
                     /gene="lepB"
                     /locus_tag="OO464_002359"
     CDS             20545..21519
                     /gene="lepB"
                     /locus_tag="OO464_002359"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6227486.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIIPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21791..22471
                     /gene="rnc"
                     /locus_tag="OO464_002360"
     CDS             21791..22471
                     /gene="rnc"
                     /locus_tag="OO464_002360"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6227487.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22468..23373
                     /gene="era"
                     /locus_tag="OO464_002361"
     CDS             22468..23373
                     /gene="era"
                     /locus_tag="OO464_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6227488.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23385..24113
                     /gene="recO"
                     /locus_tag="OO464_002362"
     CDS             23385..24113
                     /gene="recO"
                     /locus_tag="OO464_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6227489.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24125..24856
                     /gene="pdxJ"
                     /locus_tag="OO464_002363"
     CDS             24125..24856
                     /gene="pdxJ"
                     /locus_tag="OO464_002363"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6227490.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24856..25236
                     /locus_tag="OO464_002364"
     CDS             24856..25236
                     /locus_tag="OO464_002364"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6227491.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25656..25736
                     /gene="shoB"
                     /locus_tag="OO464_002365"
     CDS             25656..25736
                     /gene="shoB"
                     /locus_tag="OO464_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6227492.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25930..26190)
                     /gene="yfhL"
                     /locus_tag="OO464_002366"
     CDS             complement(25930..26190)
                     /gene="yfhL"
                     /locus_tag="OO464_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6227493.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26405..27380
                     /locus_tag="OO464_002367"
                     /pseudo
     CDS             26405..27380
                     /locus_tag="OO464_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6748 a         6818 c         7183 g         6631 t
ORIGIN      
        1 ttggcgcaga aaaaaatgcc tgatgcgacg ctgcgcgtct tatactccca catatgacag
       61 attcagcaac ggatgcggtt tccccaactt gcctacttcc atacgtgtcc tccttaccag
      121 aaatttatcc ttaagctcct caataaccat tttcctgcta actaaattca tggtttaggt
      181 tgcataatga tacgcaacaa atgtataaca ttttctttac caaaagaaat aaacaaaagc
      241 gaccaacaaa agcatcggat tacggcagga gacataatgg catggctgaa agtactgtaa
      301 cggcagacag caaactgaca agtagtgata ctcgtcgccg catttgggcg attgtggggg
      361 cctcttcagg taatctggtc gagtggttcg atttctatgt ctactcgttc tgttcactct
      421 acttcgccca cattttcttc ccttccggga acacgacgac tcaactacta caaacagcag
      481 gtgtttttgc tgcgggattc ctgatgcgcc caataggcgg ttggctattt ggccgcatag
      541 ccgataaaca tggtcgcaaa aaatcgatgc tgttatcggt gtgtatgatg tgtttcggat
      601 cgctggttat cgcctgcctc ccaggttatg aaactatagg tacgtgggct ccggcattat
      661 tgcttctcgc tcgtttattt cagggattat ccgttggcgg agaatatggc accagcgcca
      721 cctatatgag tgaagttgcc gttgaagggc gcaaaggttt ttacgcatca tttcagtatg
      781 tgacgttgat cggcggacaa ctgctagccc tactggttgt cgtggtttta caacacacca
      841 tggaagacgc tgcactcaga gagtggggat ggcgtattcc tttcgcgtta ggagctgtgt
      901 tagctgttgt ggcgttgtgg ttacgtcgtc aattagatga aacttcgcaa caagaaacgc
      961 gcgctttaaa agaagctgga tctctgaaag gattatggcg caatcgccgt gcattcatca
     1021 tggttctcgg ttttaccgct gcgggctccc tttgtttcta taccttcacc acttatatgc
     1081 agaagtatct ggtaaatact gcgggaatgc atgccaacgt ggcgagtggc attatgactg
     1141 ccgcattgtt tgtattcatg cttattcaac cactcattgg cgcgctgtcg gataagattg
     1201 gtcgccgtac ctcaatgtta tgtttcggtt cgctggcagc catttttacc gttcctattc
     1261 tctcagcatt gcaaaacgtt tcctcgcctt atgccgcttt tggtctggtg atgtgtgccc
     1321 tgctgatagt gagtttttat acatcaatca gtggaatact gaaggctgag atgttcccag
     1381 cacaggttcg cgcattaggc gttggtctgt catatgcggt cgctaatgct atatttggtg
     1441 gttcggcgga gtacgtagcg ttgtcgctga aatcaatagg aatggaaaca gccttcttct
     1501 ggtatgtgac cttgatggcc gtggtggcgt ttctggtttc tttgatgcta catcgcaaag
     1561 ggaaggggat gcgtctttag tgacgggtca gttgccagac ggtatagccg gtgcttgcac
     1621 cggcgacatc ccaggccaaa tccttccagc tccagccgct cccttcgggg cggctatccc
     1681 aaagctcttt tgacgccccc aaactgacag agaacatcaa tccaaacatg gcactgcgat
     1741 cccggctcat cccctgatgc tgtgaatatt catttccggc ggcggaaagt atcgccgagg
     1801 cgataaagtg ttgagcttta tcctgcccac tccagctatc gttatccata tggctgcatt
     1861 cagaaagtaa caatagcgaa cagacaaaaa gaatacgcaa aacgtggcct ccaaaaaaca
     1921 aaccccgtac aggacggggt tgtgattaca ggatgcggct aattaatcgg tcgatgcgga
     1981 tacggcgcaa acggcggatg agtttacgaa ccttcaccgg ataatcggca atactttgca
     2041 gatcgcgata gtgcttaacg atggtggtac gctcgcggat cagctccagt tctttctctc
     2101 gctgcggcgc aagctccagt tgtggatcgt ggatcaaaat ggcgttttcc agatccagac
     2161 gccaggcgcg cgggttcagg ttattaccgg tgatcaacat ccacttatca tcaacccaca
     2221 tccctttcag gtgataggtg ttgtcgtcat ctttccataa ccgaaccact agctggtcag
     2281 tattgacgta atactgcaaa cggctcagga aacgacgcag attgatctca tagagataag
     2341 gcaatgcgcc aattatcttg aaaggttcat cttccggaat gtagaagtcg ttcgcggttt
     2401 tatcaccaac aataatttcg acctttttcc cttcgcgcag caactggata atattgcgca
     2461 caaggattgc tggcaggttg aagtatggcg tacagatggt tagtttctgc tcggcacaag
     2521 gcataagatg gaaaatggtc ttgttcaaca gactcgattt ccccagcccc actagcggcg
     2581 ttacggaaag ctgatcgttg tcggcatcgc cctggaaatg ataagcggca tcacgcagct
     2641 cctggcggaa cagacgaata tcgttcttga tttccgggct ttttggccga ttaacatcat
     2701 ccagacgatt aacgccgcgg ccattcataa tattctgtgt aacccattca aacataatgt
     2761 ctgacatctt acggttacgg atcagatgat aacggtcgta gcgatattta tcgtgttgat
     2821 gcaggtaaac atcgttcagg ctggcaccgc tataaagtac gctatcgtcg atgataaagc
     2881 ctttaaagtg cagaacacca agagcttcac gggtattgat tggaacgcca taaaccggaa
     2941 catctacgcc cggattttcc tgcgccatgc ggcagtacca gtcagcgtta gtgttagatg
     3001 ccgcagcgcc aatgcgtcca cgttgtgcac gatgccagtc gaccagcacc cgcacatcca
     3061 gttccggacg ctgcctttta gcctcataca acgcgttcag aatgcctttg ccaccgtcat
     3121 cctgttcgag atacagggcg acaatgcaaa tgcgctgctt cgcgctggct attttttcca
     3181 gcagcgtctc ccggaagtcg gcgggagcgt aaaagaaatc gacatcatca actgattgag
     3241 aaatcttggg tagttgggca aggtgttgtt gatgtttatt acgcttaaat tttgacaaca
     3301 tcacagtgca tttcttctct gttcattgaa gggtcctctg tgcaatgcag acgacataag
     3361 cgggcaataa taacaccagt cccgattaag tggtcaacat ttccagtacc ttactcatga
     3421 ttcctcgcgc cgggcaagat ttagcgtaag cccaacgatc ccctcttcga gctggatatc
     3481 aacgttaaac ccgagcttgc gggctagcgc caccatgcca cgattgtttg gcatcgtaat
     3541 accattcaga cgttgtagtc cgtgatttcg cgtataggta atcaactttt ccattaagcg
     3601 tcgacctaag cctaaccctt tgagatccga gcgaaccagt acagcaaatt cggcatcgat
     3661 gttatcagga tcggaaatcg cacgcgtgac gccgaggatc tcttccgttt gatcaatacg
     3721 tcgtaccgct acaaacgcca tttcccgatc gtagtcgatc tgtgtcatgt tggctaaatc
     3781 ttcatgggta aattcgttga tctcgctaaa gtagcggtaa taaagatctt ctttggtgac
     3841 tcgcgaaatg aattgctgaa gttgtggctc atcttctggc aaaatcgggc ggaacaagca
     3901 gcgttcaccg tttttcaatt ctacccattc ttccagctga tgcggataag ggcgcactgc
     3961 cagccgactc tcgttatcgc cttcaaacgg cgagatatcc agcgtgacat ccagcgcggt
     4021 aaattcactg ccagaagcca gcaaaggatg aatatccaga cgctgaattt ccgggcaatc
     4081 gacaatcaag ttggaaacct gcaccagaag ctggctcaag cctgcaacat ccaatgggcg
     4141 tagcgcactg cgcgcacgaa tctttttact tttgatcccc tgaataacca gatagcgggc
     4201 caggttcatg ttcagcggcg gcagtgcgac gacggcttga tcttcaggac gccactccac
     4261 accgccttca cccagcatga tcaacggccc gaaaaccgga tcgtgctcaa ccacaacccg
     4321 caactcctga gcgccagcac ggttagccat actttgcacc aacaggccgt ggacccgcgc
     4381 ctgtggccag gccattttta cgcgatcgaa aatagcgttc gccgcttgct ggacttcatt
     4441 ggctgtacgc aggtaaagca tgacgccctg aacttccgat ttatgtggaa tatccggcga
     4501 acgcaatttc agcgccaccg gataaccaat ctgttcggca atatgcaccg cttcggtgct
     4561 atcgctggca atccaggttg ggagcgtgtt catgccatac gcttgcagga tgggctgaac
     4621 ttcatgggta tcgagcgacg tagccccttc ggcaatcgcc tgttgcaaca gaagatgcgc
     4681 ttctgcggta ttggaagtca gattgctggg caacgccggc gtttcgcgta gttgcttctg
     4741 attacgccgg tactccacca tatgcataaa agcagtgatg gttccttccg gggtacggta
     4801 ggtcggcagc ccggcttcgc tgaataaacg tcgtgcctct tgcgaggagt gctcgccgca
     4861 ccagttcgtc agcaaagaga catatttgct gcggggatga tgctttaccg cttcaattaa
     4921 tacttgcgcg ctttctgttg cgggagcagc ggcgctgggc gaatgaataa ccatcagtgc
     4981 atcaaaatcc tggctgtgga gcagaatgtc cagcgttttg acataatgct cgctgctggc
     5041 gtcatcgcgc agatcgagcg ggttagagac tgccacatgt tctggcaatg catcgcgcaa
     5101 tttctggcag gtttcttcgc ttagcgttgc cagcttgcca ttgcgtgacc ataaggcatc
     5161 cagcgccagc gcggcaggcg cagcaccgtt gctgataatc atcaaccggt cgccacgcag
     5221 cgggcgcata tggctaaggg tttccaccgc cgaaaacagt tcgtgggtat cctgtacccg
     5281 caacaaaccg gcacgctgaa tagccgcatc ccatgccggg tccattcctg ccgtggtgtt
     5341 gagcagtcgc tgtgccgccg ggctacgtcc gcttttaatc accagaatcg gtttattacg
     5401 cgaggcacta cgggccgccg aaacaaagcg tcgcgcgtcg cttaactgtt cgagatagag
     5461 caggatggcg ctggttttac tgtcgcgcgc cagatagtcg agcaattcat caacgtcgat
     5521 atctaggctg tcgccgagcg caataaagta ggaaaagccc atctcacgct gttgcgccca
     5581 gtcgaggatg gtgttggaga cggcagccga ttgcgaaata aacgccagct tgccgcgttt
     5641 aatcggcaca ggcgaaaagc tggcattcag accttgccag ggagccagta aacccagact
     5701 gtttggtcca agcaggcgca tgttatggcg cagggcgcag gcgcggagat cttcgtgttg
     5761 cgatgccggg gcggaaagaa taatgcaggt tttacagcct ttctcgccga gctcttccag
     5821 aagagcaaga ttacggctgg cattggtaca taaaaccgca aggtcgggtg taaagggcaa
     5881 gctggcaata tccggccagg ccaacacacc caacaccgct ttccaggctg gcgtcaccgg
     5941 gagtaccggt ccgttaaagc ctcccgccag caggttacgc atcatcaggt aacctgcgcg
     6001 attgggtttc atcgacgcgc caattaccgc tatcgatttt ggtcgcagta gtgcttccag
     6061 tcctcgctga ctcataccgg tctcccgcag aagtgaccga atgattttaa acgctttcta
     6121 actgttctgc tgtgatgcta cccagatgtt gcgtttttcc tgccagatag cgtgttttaa
     6181 agcgggtaaa atgctcgcct aaccctgctg ccgccccggt atcgccggcc atatctaaca
     6241 gtgcgatggc tacctcggca gtacaatatt ggcctgcagc ctgggcttca cgcaggcgat
     6301 aggcagaaag ccgggaaaga tcgacggaaa tgacgggaag attatccaga tacggacttt
     6361 tacgaaacat cttgcgagct tccggccagg taccatcgag catgataaac agcggtggct
     6421 taccggcagg tggtgtgaag atcacttccc gttgctcatc agcatacgag gcgggaaaga
     6481 ccaccattgg ctgataatcc gggttttgta ccagatccag caaatcctgc gagggttcgg
     6541 tacgcgacca ttgaaacgca acggtatcag gcaaaatatc agcaatcaga cgcccggtat
     6601 tactgggctt cattggctcg gtgtcgaaca tcagcaaaca gaagcgactt tttgcttgtg
     6661 ctggggtaat tgtcgaacag agacataatt tctctggcaa aagacagcgt tggcagcgac
     6721 gaacgcgatt accgcgggca agaaaaggac gtgttgcgcg cgcaatacgc tcggcgcgta
     6781 actggagaac agcgttttcg gtcataagag agcgtcgaaa aaacgccatt gtcgcagagg
     6841 agaaaacggg gcacaagatg cgccccggta agattaaaga gattcgttca gccagctatc
     6901 gaacggcgct ttcggtactg cgccattaag catgtcgaca acctgaccgt ttttgaaaat
     6961 catgatcgtc ggtatactac gaattccaaa gcgactgctc aattcacgtt cagcttcggt
     7021 attcactttc acaaagcgca ctttaccgct acgctcttgc gcgacatctt caaaaattgg
     7081 tgcgaaatta cggcaggggc cgcaccacgg tgcccagaag tcgatcacca caggtagatc
     7141 atccttcagc aatttgtcga gtgtttcacc ggtcgcatta atcacctctc cgtcaaacaa
     7201 gtcgtgaccg cagcgtccgc attttgccgc atcttcgatc cgatcgtcgg gaatgcgatt
     7261 gatggcctga caatgggtac aaacggtatt cataactaac ctcgggatga gtaaggagac
     7321 ttatcggcaa cgacgccggt atgtttctaa tatgttacat attatcgggg acattgttcc
     7381 agacaacaat ctgttttatt caataggtat gatagtcgca ggctttgcta cgaaatcatg
     7441 gcgaaccgcg cggacttcgg gtaatctgcg cgcttcgcgc agcgctggtg gagaaaagca
     7501 tgaacgatga aatgaaaggt aaaagcggca aggtcaaagt gatgtatgtc cgcagtgatg
     7561 atgattctga taaacgtacc cacaacccgc gtaccgggaa agggggcggg cgtccaggaa
     7621 aatctcgtgc tgatggtggc cgtcgccccg cccgcgatga caaacagagt cagccccgtg
     7681 accgcaagtg ggaagattcg ccgtggcgca cggtttcccg cgcgccgggt gatgagacgc
     7741 cggaaaaggc cgatcacggt ggcatcagtg gtaaaagttt tattgatccg gaagtgttgc
     7801 gtcgtcagcg tgcggaagaa acccgcgtct acggcgaaaa cgcctgtcag gcactgttcc
     7861 agagccgtcc ggaagcgatt gttcgcgcct ggtttatcca gagtgtaacg ccgcgtttta
     7921 aagaagcctt gcgctggatg gcagcaaacc gcaaagcgta ccatgtggtg gatgaagcgg
     7981 aattgacaaa agcgtcaggt acggaacatc acggcggcgt ttgcttcctg atcaaaaagc
     8041 gtaacggtac aaccgtgcag cagtgggtaa tccaggcagg cgcgcaggat tgcgttctgg
     8101 cactggaaaa cgaatctaac ccgcataacc tgggtggcat gatgcgcagc tgcgcgcact
     8161 tcggcgtgaa aggtgtcgtg gtgcaggatg cggcactgct ggagtcgggg gcggctatcc
     8221 gtaccgcaga aggcggcgca gagcacgttc agccgattac tggcgacaac attgttaacg
     8281 tgctggatga tttccgtcag gcaggttaca ccgtagtgac cacttccagc gagcagggta
     8341 aaccgctgtt caaaaccagt ctgccagcga aaatggtact ggtgctgggt caggaatatg
     8401 aagggttacc ggatgccgca cgcgatccga acgatctgcg cgtgaagatt gacggtacgg
     8461 gcaacgttgc cgggctgaat atctctgtcg caaccggcgt tttgcttggt gaatggtggc
     8521 gtcagaataa agcctgataa atcagccggg tggcaactct gccatccggc atttccccgc
     8581 aaatttactc acactctgcc ggtaatactg gcatccagtc aatcggcgtc tcgccacgtt
     8641 gttccagcca ctgatttgcc agcacaaaat ggttgcagcc aaagaatcca cgatgcgccg
     8701 aaagcggcga cggatgcggt gctttcagta catgatggcg ttgcttatct ataatcgccc
     8761 ctttcttttg cgcatgtgat ccccacaaca aaaacaccac gccttcgcgg tgctggttaa
     8821 tcaggctgat gactttatca gtaaacgtct cccaaccgag gctggcgtgg gaatgcgcct
     8881 gacctgcgcg aaccgtcaac acagtattga gcagcagaac gccctgacgc gcccagcttt
     8941 cgagatagcc atgattaggg cgggtgaagc ccggaatagt attttccagc tctttataca
     9001 tattcaataa tgacggagga atggcaatgc cgggacgaac ggaaaatgcc agaccatgcg
     9061 cctgtcccgg tccgtgataa ggatcctggc cgagaatcac cactttaacg tcacccaact
     9121 cggtaaagcg gaacgcgtta aagacatctt tttgtggtgg atagatagtg acgccggact
     9181 gccgctcgct ggcgacggtc tgaagggtat taagaaaata aggttgttgc ttctcttcag
     9241 ccagcacgtc atgccaggtt aattcgttag ccatcttgct ctcctgcgaa tcttcaatcc
     9301 gcctagctta actgcttctt tctacagaac aaaatcatgc tcatttttta gacttaaacg
     9361 agtgcaaaaa aatgtaaatt ggtaaaattt cacacccgca taagttgatg taaaacaata
     9421 aaatccattt acgaccacaa tcgatgtgtg gtttttgttg atttaaatca aagaatcaag
     9481 ggtggttgag gagtatatat acactcaagc aacaatggtt ttaccaattg gccgcgacag
     9541 gctgaacaaa tcaaataatt ttgccgggga ggcatcacat gattacaggt atccagatta
     9601 ctaaagccgc taacgccgat ctgctgaact ctttctggct gctggacagc gaaaaaggcg
     9661 aagcacgttg catcgttgca aaagcaggtt atgcagaaga tgaagtggtt gcagtaagca
     9721 aactgggtga cattgaatac cgtgaagttc cagtagaagt gaaaccagaa gttcgcgttg
     9781 aaggtggtca acacctgaac gttaacgttc tgcgtcgtga aactctggaa gatgcagtta
     9841 agcatccgga aaaatatccg cagctgacca tccgtgtatc cggttatgca gttcgcttta
     9901 actctctgac tccggaacag cagcgcgacg ttatcgctcg tacctttact gaaagcctgt
     9961 aaggcttaag tagcaagtta ctaagcggaa gagggcgacc tcttccgctt tttttcgtta
    10021 atagaaaatg cgtaccgcgc aatagaccag caacagggca agcacgatat ttaactggcg
    10081 accatactgg cgaaacaatc gctgaaacag atgccccgcc agcgcccagc acacattgcc
    10141 aaacgtccca atcatcgcca gcaaaacgct gacgccaact acccagctta gcgcctgtgt
    10201 ttgcggcaga acaaacgtcg acagtgccgt aacaccgtac aaaatgattt tgacgttcac
    10261 aaactgcaaa gcaaagctgg cccaaaagct gattggtttt gcctgaagtc cgtcttcctt
    10321 tgttgggctg gtggcgattt tccacgccag ccagacaata tatgccgccc ccgcccaact
    10381 caaaaggtgt accgctgccg ggtcaatcac tgccagtgaa aatgaaatgc ccgcacacag
    10441 taacatcaca atcaaaaatc ccagactcat ccctgccagc acgcgggtac tttgacgaaa
    10501 tccatgcgac gtagcagagc taagggcgag aatattgttc ggtcctggcg tcatagcggt
    10561 aatcagggtg taagtccaaa aagcacttaa aaggatcggt gtcacttgtt atctcctctg
    10621 ttttggtaga cagtacccga cacggaacat ttaaaaaaat gaatgttttt atattctaaa
    10681 ttcgatattt tcgataggtt tggggttatg gatctgcgcc gttttattac gcttaaaacc
    10741 gtggtggaag agggttcctt tttgcgagct tcgcaaaaat tgtgctgtac acaatcgacg
    10801 gtgacttttc atattcagca gcttgagcag gagttttcag tccagttatt tgagaaaatt
    10861 ggccgacgga tgtgccttac ccgcgaagga aaaaagttgc tgccgcacat ttatgaactt
    10921 acccgagtga tggatacgct tcgtgaggct gccaaaaagg agtcagatcc ggacggggaa
    10981 ctacgcgttg tttcgggcga aacgttgctc tcttaccgaa tgccgcaggt gttgcagcgt
    11041 tttcggcaac gcgcaccaaa agtgcgttta tcgttgcagg cgctgaactg ttacgtgatc
    11101 cgtgatgcgc tactgaatga tgaggccgat gtcggcgtct tttatcgcgt agggaatgat
    11161 gatgccctga accgacgaga gttgggtgaa caatcgctgg tgctggtggc ttcaccacaa
    11221 attgcagatg tcgattttac tgagccggga agacataacg cctgtagctt tattatcaac
    11281 gaaccgcaat gtgtcttccg gcagatattt gagagcacgc tgcgacagcg gcggatcacg
    11341 gtggaaaaca ccattgagct tataagtatc gaaagcatca agcgttgtgt cgcggcgaat
    11401 atcggtgtca gttacttgcc ccactttgcg gtggaaaaag agattgagag cggagaatta
    11461 attgaattgc cttttggcga acagtcgcag accattacgg caatgtgcgc acattatgct
    11521 ggaaaagcgg ttagcccggc gatgcacatt tttattcagt gtgttgaaga gtgttttgtg
    11581 gttgcgtaaa aaaacaatgc cgggcttaaa cccggcattg agattactct tctgtcgttt
    11641 gcggtggcac gcccgttccg ctcggtttac ggcgcttacc aatatttttg gtgtcgcgat
    11701 ggcgtttttt cacccgcggc ttctctttct ctttagcttt tttcttctcg gcgcgttttg
    11761 ccagtacttt cttcgatggc ttgccggtct gcttctcgct tggcgcacgc gttttcgggc
    11821 gtaactcatc aataacgcga gctttaatcg gctcttcaat gtaacggcca accttgccca
    11881 gcagcaaatg gtcatgggct tccaccagcg aaattgcggt gcctttacga ccggcgcgcg
    11941 cggtacgtcc gatacgatgc aaataagtat cgccactgcg cggcatatcg aagttaaaga
    12001 cgtggctgac gtcaggaatg tcgataccgc gcgcggcaac atcggtggca accagtacgt
    12061 ttacgcggcc ttcggtcagg cgtttaattg cttcgttacg cttgccttgt accatctcac
    12121 cttcgagata gcagttgttg atgcccgctt cgcgcagcca gtttgccagc tcatgcacac
    12181 gttcacgctt acgcacaaac acgattgagc gagtcgcttc aggctgtttt aacaggtgca
    12241 ccagcaatgc ggttttatgt tccaggtcat cggcgcggta ataccactga tgaatttttt
    12301 tgcgctcacg ggtggacgga ttggcagaaa cttccaccgg atcttccagc agacgctcgg
    12361 caaagtcctg aatcgcatcg ccttccagcg ttgccgaaaa gagcagggtc tgtttacgcc
    12421 agcgagtttc gccagcaata tgttcgatat cctgagcgaa gcccatatcc agcatacggt
    12481 ctgcttcgtc gaggatcagc gtttcaaccg cgcggcaatc gaagttctct tcttttatgt
    12541 attgcagcag acgtccggtc gtggcgacca cgatgtcctg attttcgctg aacacttccg
    12601 cgtggttcat ataggctacg ccgccggtga tggtggcgat atccagatgc gtatgtttcg
    12661 ccagttcgcg ggcatgatcg gccacctgca tcgccagctc gcgagttggg gtgaggatca
    12721 aaatacgcgg cggaccggat ttcttacgtg ggaaatcgag caggtgctgc aacgctggca
    12781 gcagatacgc cgccgtttta ccggtgcctg tcggcgcaga accgagtaca tcacggccat
    12841 cgagcgcagg cggaatggcg gcagcctgaa tggcggtcgg gcgagtgaaa cctttatcct
    12901 ggagggcttc cagcaggctt tcgtcgagtt caagttcgga aaaagtcgtt acagtcatgt
    12961 tctacctctg tgtggggcgc tgattataga cgttacggct gcaatcttca tctgtttgta
    13021 tggatatcgc ttctcgggta ttgttctcac ccggtgctat cgccgttttt cctgcaaggt
    13081 tttattttca tgtcacagtc tacatccgtg cttcgtcgta atggatttac ttttaaacag
    13141 ttttttgttg ctcacgatcg ctgtgcgatg aaagtgggaa cggatggcat tttgctgggc
    13201 gcatgggcac cggtggctgg ggtgaaacgt tgccttgata tcggcgcggg tagcgggttg
    13261 ctggcactga tgctggcgca gcgaaccagt gacagcgtga taattgatgc cgttgagctg
    13321 gaaagtgaag ctgccgcgca ggcgcaggaa aatatcaacc agtccccgtg ggcagagcgg
    13381 attaacgtcc atacggcgga tattcagcag tggatcacac agcagacagt acgcttcgat
    13441 ttaatcatca gtaacccacc ttactatcag cagggagtag agtgcgcgac acctcaacgg
    13501 gaacaggctc gctataccac tacgcttgat cacccatcgc tgctgacctg tgcggcggag
    13561 tgcattaccg aagagggatt tttctgcgtg gtgctgccag agcagattgg taatggtttt
    13621 actgaactgg cattaagcat gggctggcat ttacgtttgc ggacggatgt ggcggaaaac
    13681 gaagcgcgac tgccgcatcg ggtgctgctg gcattctccc cgcaggcggg agaatgcttt
    13741 agcgatcgct tagtcattcg tgggccagac cagaactatt ccgaagcgta tacggcgctg
    13801 acccaggctt tttatctgtt tatgtaatga ttgccggggg aaaggatcga tggaccggaa
    13861 tgcgtgagca gttccgggta atccagcgta aaatgcagcc cccggctttc tttgcgcatc
    13921 atcgcgcagc gaacaatcaa ctcggcaacc tgtaccagat tacgcagctc cagcaaatta
    13981 tttgagacgc ggaaatgggc gtaatattcg tctatttctt gttggagcat ggttatccgc
    14041 cgcagggcgc gttccaggcg cttcgttgtg cgcacaatgc caacgtaatc ccacataaac
    14101 agccgtagct cgtgccagtt atgctgaatt actacccgtt cgtcagggtt ctcaacgcgg
    14161 ctttcatccc acggcggtaa cgtactgacg ccgtgggcat aaggcatacg tctggtgata
    14221 tcttctgccg ccgaccagcc atagaccaga cactccagca atgaattcga ggccatgcgg
    14281 ttagcgccgt gtaagccggt ataactcacc tcgccaatgg catacaagcc ctcgacgtcc
    14341 gtacgcccat gatcatcaac cattacacca cctcaggtat agtgtgcagc aggcacaatc
    14401 ggtaccggtt cttgtgtgag atcaatcccc agcccgagca atttttcata aatcatcggg
    14461 aaatgctggc gaataaaatc ggcgggctta tggctgatgt cgaggaacat acaatctgcg
    14521 ccgaggcgtt tcatttcatg gtcaatggcg cgggcgacaa tatcgcgcgg ggccagttcg
    14581 ccgcgctcat caaaatcggg cataaaacgc gtgccatccg ggcgcttgag ataagcgcct
    14641 tcgccgcgca gtgcttccgt taacaggaaa ttgcgtgcct gtgggtgata cagtgcggta
    14701 gggtggaact gattaaattc gagattggca acccgacagc ccgcgcgcca cgccatagca
    14761 atgccatcgc cagaagaaat atccggattg gtggtgtact gatacacctt cgacgcaccg
    14821 ccggttgcca gtaccactgc ttttgcgtgg caggtttcca ccgtttcttt atttcggttc
    14881 catacccacg cgcctacaac ccgtcgcgtg cccggcaggc caattttgtc agagacaatc
    14941 agatcaactg cgttgctgcg ctccagcaca cgaatattcg gatggttctg cgccttgctc
    15001 accagcgtgg attgtacttc tctaccggtg gcgtcggcgg catgaagaat acgacggtga
    15061 ctatgtccac cttcacgggt cagatggtag ctttcttcgc cattcggttg aacgtgggta
    15121 tcaaacaaaa ccccctggtc aattaaccat ggcacacagg aacgagcatt gctggcgaca
    15181 aattcaactg catggtgatc gcaaatacca gccccggcaa tcaatgtgtc ttccacatgc
    15241 gagtcaatgc tgtcagtttc atcaaacacg gcggcaatac cgccctgggc ataaaatgtt
    15301 gaaccttccg ttaccgggcc tttacttaga acgatgacct gatgctggtc agccaggcgt
    15361 agcgccagtg aaagtccggc tgcgccgcta ccaataatca acacgtcaca tgaatgttca
    15421 gggagagtat tcattttctt tgtttaattt actaaacatt gtttggtcgg catagcatca
    15481 tgttgtgcga ataaacacct gctattttaa tatttgttac agtggctaaa cacgttgacg
    15541 cagggcggcg agaaaaagag aagttactgg ctggtggagg attaggtggt gaaataaaaa
    15601 ggccgttggg ttactcttca ggcagttaaa tgggcatttc tacacagata atgcgatgtt
    15661 cagattctgt agacttataa tgatagataa tgatccgtct acagcatgac aaacaaaaac
    15721 agatgcgtta cggaacttta caaaaacgag acactctaac cctttgcttg ctcaaattgc
    15781 agctaatgga gtggcgtttc gatagcgcgt ggaaatttgg tttggggaga ctttacctcg
    15841 gatgagcgag cagttaacgg accaggtcct ggttgaacgg gtccagaagg gagatcagaa
    15901 agcctttaac ttactggtag tgcgctatca gcataaagtg gcgagtctgg tttcccgcta
    15961 tgtgccgtcg ggtgatgttc ccgatgtggt acaagaagct tttattaaag cctatcgtgc
    16021 gctggattcg ttccggggag atagcgcttt ttatacatgg ctgtatcgga ttgctgtaaa
    16081 tacagcgaaa aattacctgg ttgctcaggg gcgtcgtcca ccttccagtg atgtggatgc
    16141 cattgaagct gaaaacttcg aaagtggcgg cgcgttgaaa gaaatttcga accctgagaa
    16201 cttaatgttg tcagaagaac tgagacagat agttttccga actattgagt ccctcccgga
    16261 agatttgcgc atggcaataa ccttgcggga gctggatggc ctgagctatg aagagatagc
    16321 cgctatcatg gattgtccgg taggtacggt gcgttcacgt atcttccgag cgagggaagc
    16381 tattgataac aaagttcaac cgcttatcag gcgttgacga tagcgggata ctggataagg
    16441 gtattaggca tgcagaaaga acaactttcc gctttaatgg atggcgaaac gctggatagt
    16501 gagctgctta acgaactggc tcataaccca gaaatgcaga aaacctggga aagctatcac
    16561 ttaatccgtg attcaatgcg gggtgatact cccgaggtgc tccatttcga tatctcttca
    16621 cgcgtgatgg ccgccattga agaagagcca gtacgtcaat cggcgacatt gatcccggaa
    16681 gcccagcctg cgccgcatca atggcagaaa atgccattct ggcagaaagt acgtccgtgg
    16741 gcggcacagc ttacccaaat gggcgtagcc gcatgcgtat cgcttgcagt tatcgttggc
    16801 gtccagcact ataatggaca atctgaaacg tcccagcagc ccgaaacgcc ggtatttaat
    16861 acactgccga tgatgggtaa agccagcccg gtaagcctgg gagtaccttc tgaagcgacc
    16921 gcaaacaatg gtcaacagca gcaggtacag gagcagcgtc gtcgcattaa tgcaatgttg
    16981 caggattacg aactgcaacg ccgactccac tctgaacagc ttcagtttga gcaggcgcaa
    17041 acccagcaag ccgctgtaca ggtgccagga attcaaactt taggaacgca atcgcagtaa
    17101 tgaagcaact ttggtttgcc atgtcattag tgacaggtag cctgttattc tctgctaacg
    17161 cctcggccac tcccgcgtcc ggggcgttat tacagcagat gaacctggcc agtcagtcac
    17221 tgaattacga gctgtcattc atcagcatca ataaacaggg tgttgagtct ctgcgttatc
    17281 gacatgcacg cctcgataac cgtcctcttg cacaattgtt gcaaatggat ggcccgcgcc
    17341 gggaagtggt acagcgcggc aatgaaatca gctattttga accgggactt gaaccgttca
    17401 cgcttaatgg cgattacatt gttgattctc tgccatcgct tatctatacc gatttcaaac
    17461 gcctttctcc ttactacgac tttatctccg tcgggcgcac gcgtattgct gatcgtcttt
    17521 gcgaagtcat tcgcgtggtt gcccgagatg gtacacgcta cagctacatc gtgtggatgg
    17581 acaccgaatc gaaattaccg atgcgggttg atctccttga tcgcgatggt gaaacgctgg
    17641 aacaatttcg cgtgattgct tttaacgtca atcaggatat cagcagcagt atgcagacgc
    17701 tggcgaaggc aaatttgccg ccgttgcttt ctgttcctgt aggtgaaaaa gctaaattca
    17761 gctggacgcc aacctggttg ccacagggtt ttagcgaagt ttccagtagt cgacgtccgc
    17821 taccgacgat ggacaacatg cctatcgaat cacgtctcta ttccgacgga ttattcagct
    17881 tctcggtaaa cgttaaccgc gctacgccat cgagcaccga tcagatgttg cgcaccggac
    17941 gcagaaccgt cagtacaagc gtacgtgata acgccgaaat caccattgtc ggtgaactgc
    18001 cgccgcaaac ggcgaaacgc attgccgaga atattaagtt cggggcagcg caatgatcaa
    18061 agagtgggct accgtcgtct cctggcaaaa cgggcaggcg ctggtcagtt gtgatgttaa
    18121 agcttcatgc agcagctgcg cttcacgcgc cggttgcggt agccgcgtgt tgaataaact
    18181 tggcccgcaa accacgcata ccattgtcgt accctgtgat gagccgttag tgccggggca
    18241 aaaagtggag ttggggatcg ccgaaggcag cctgcttagc tccgcattac tggtttatat
    18301 gtcgccgctg gtgggattat tcctcatcgc ttcgctattt cagctactct ttgcttctga
    18361 cgtcgcagca ttatgtggtg cggtcctcgg cggtattggc gggttcctga ttgcgcgcgg
    18421 ctactcgcgt aagtttgctg cccgggcaga atggcaaccg atcatcttaa gcgtggcatt
    18481 gccgcctggc cttgtgcgat ttgaaacatc ttcggaagac gcgagccagt gatttcactg
    18541 gctttgttgc attttgcata acaaatgcga ggacgtttcc agataccaca atccatacgg
    18601 tatgaacatt tcctcgcctc aatgttgtag tgtagaatgc ggcgtttcta ttaatacaga
    18661 cgttaagctc agaacagcga cttctaaaag cctggttaac cgggcattaa ggcacaataa
    18721 tcatacttta tgaagaatat acgtaacttt tcgatcatag ctcacattga ccacggtaaa
    18781 tcgacgctgt ctgaccgtat tatccagatc tgcggtggcc tgtctgaccg tgaaatggag
    18841 gcgcaggttc tcgattccat ggatcttgag cgtgagcgtg gcattaccat caaagcgcaa
    18901 agcgtgacgc tggactacaa agcgtctgac ggcgaaacct atcagcttaa ctttatcgac
    18961 accccgggcc acgtagactt ctcctatgaa gtttcccgtt cgctggctgc ctgtgaaggt
    19021 gcattgctgg tggtcgacgc cgggcagggc gtagaagcgc aaaccctggc aaactgctac
    19081 accgccatgg aaatggatct cgaagtggtg ccggtactga acaagattga cctgccggca
    19141 gccgatcctg aacgcgtggc ggaagaaatt gaagatatcg tcggcatcga cgccaccgat
    19201 gcagtgcgct gttcagcgaa aaccggcgtt ggcgtgcagg acgttctcga acgtctggtg
    19261 cgcgacattc cgccgccgga aggcgatccg gaaggcccgt tgcaggcact aattatcgac
    19321 tcctggttcg acaactacct gggcgttgtt tcacttatcc gtattaaaaa cggcaccctg
    19381 cgtaagggcg acaaagtgaa agtcatgagt accgggcaga cctataacgc cgaccgtctg
    19441 ggcatcttca cgccgaaaca ggttgaccgc actgaactga aatgtggcga agtaggctgg
    19501 ctcgtatgtg cgattaaaga tatccacggc gctccagtcg gcgatacctt aacgctggcg
    19561 cgtaatccgg cagaaaaggc gctgcctggc tttaagaaag tcaaaccgca ggtatacgcc
    19621 ggtctgttcc cggtaagttc cgacgactat gaagccttcc gtgacgcgct gggtaaactc
    19681 agcctgaacg atgcctcact gttctatgag ccggaaagct ccagcgcgct gggctttggt
    19741 ttccgctgcg gcttccttgg cctgctgcac atggagatca tccaggagcg tctggaacgt
    19801 gaatacgatc tggatctgat caccactgcg ccgaccgtag tgtatgaagt tgaaaccacg
    19861 tcaagggaag ttatctacgt cgacagccca tccaagctgc ctgcggtaaa taacatttac
    19921 gaactgcgcg agccgattgc agagtgtcac atgctgctgc cgcaggcata tctcggcaac
    19981 gttattacgc tgtgcgtaga aaaacgcggc gtgcagacca atatggttta ccacggtaat
    20041 caggtggcgc tgacgtacga gatcccgatg gcggaagtgg tgctcgactt cttcgatcgc
    20101 ctgaaatcta cctcgcgtgg ttatgcgtct ctggattaca acttcaaacg cttccaggcg
    20161 tccgacatgg tacgtgtaga cgtattaatc aacggtgaac gtgttgatgc gctggcgttg
    20221 atcacccacc gtgataattc gcaaaaccgc ggtcgcgagt tggtggagaa gatgaaagat
    20281 ctgatcccac gccagcagtt tgatatcgcc attcaggcag cgattggtac gcacattatt
    20341 gcgcgatcca ccgtgaaaca gctgcgtaaa aacgtactgg ctaaatgtta tggcggcgat
    20401 atcagccgta agaaaaagct gctgcagaag cagaaagaag gtaagaaacg catgaagcag
    20461 atcggtaacg ttgagctgcc gcaggaagcg ttcctcgcca ttctgcacgt cggcaaagac
    20521 aacaaataac ccttaggagt tggcatggcg aatatgtttg ccctgattct ggtgattgcc
    20581 acactggtga cgggcatttt atggtgcgtg gataaattct ttttcgcacc taaacggcgg
    20641 gaacgtcagg cagcggcgca ggcggctgcc ggtgactcac tggataaagc aacgttgaaa
    20701 aaggttgcac cgaagcctgg ctggctggaa accggagctt ctgtttttcc ggtgctggct
    20761 atcgtattga ttgtacgttc gtttatttat gaaccgttcc agatcccgtc aggttcgatg
    20821 atgccgactc tgttaatcgg tgattttatt ctggtagaga agtttgctta tggcattaaa
    20881 gatcctatct accagaaaac gctgatcgaa accggtcatc cgaaacgcgg cgatatcgtg
    20941 gtctttaaat atccggaaga tccaaagctt gattacatca agcgcgcggt gggtttaccg
    21001 ggcgataaag tcacttacga tccggtctca aaagagctga cgattcaacc gggatgcagt
    21061 tccggccagg cgtgtgaaaa cgcgctgccg gtcacctact caaacgtgga accgagcgat
    21121 ttcgttcaga ccttctcacg ccgtaatggt ggggaagcga ccagcggatt ctttgaagtg
    21181 ccgaaaaacg aaaccaaaga aaatggaatt cgtctttccg agcgtaaaga gacactgggt
    21241 gatgtgacgc accgaattct gacagtgccg attgcgcagg accaggtggg gatgtattac
    21301 cagcagccag ggcaacaact ggcaacctgg attattccgc cgggacaata cttcatgatg
    21361 ggcgacaacc gcgacaacag cgcggacagc cgttactggg gctttgtgcc tgaagcgaat
    21421 ctggtcggtc gggccacggc tatctggatg agcttcgata agcaagaagg cgaatggccg
    21481 actggtgtgc gcttaagtcg cattggcggc atccattaat agccatcttc gttcacgttg
    21541 tcgccgttat ggcgacaacg tgaattattt atgagataaa tctcccgtgg ctaacgacat
    21601 cccccgtcgt tgtgtataga atattccccc gaagtttaag gttggcacct tcaggttgcc
    21661 acggcacacg aaacagcgtt ggtccccata taccgctaaa ctgaaactgc agcgaagcag
    21721 ttagcagagc catgtatatc aggtctgttt cgtgtgctga attgttgacg catttattta
    21781 ttggtatcgc atgaacccca tcgtaattaa tcggcttcaa cggaagctgg gctacacttt
    21841 taatcatcag gaactgttgc agcaggcatt aactcatcgt agtgccagca gcaaacataa
    21901 cgagcgttta gaatttttag gcgactctat tctgagctac gttatcgcca atgcgcttta
    21961 tcaccgtttc cctcgtgtgg atgaaggcga tatgagccgg atgcgcgcca cgctggtccg
    22021 tggcaatacg ctggcggaac tggcgcgcga atttgagtta ggcgagtgct tacgtttagg
    22081 gccaggtgaa cttaaaagcg gtggatttcg tcgtgagtca attctcgccg acaccgtcga
    22141 agcattaatt ggtggcgtat tcctcgacag tgatattcaa accgtcgaga aattaatcct
    22201 taactggtat caaactcgtc tggacgaaat tagcccaggc gataaacaaa aagatccgaa
    22261 aacgcgcttg caagaatatt tgcagggtcg ccatctgccg ctgccgactt atctggtagt
    22321 ccaggtacgt ggcgaagcgc acgatcagga atttactatc cactgccagg tcagcggcct
    22381 gagtgaaccg gtggttggca caggttcaag ccgtcgtaag gctgagcagg ctgccgccga
    22441 acaggcgttg aaaaaactgg agctggaatg agcatcgata aaagttactg cggatttatt
    22501 gccatcgtcg gacgtccgaa cgttggcaaa tccacattgt tgaacaaact gctggggcag
    22561 aaaatctcca tcacctcccg caaggcgcag acaactcgtc accgcattgt ggggatccat
    22621 actgaaggcg cgtatcaggc gatctacgtc gatacaccgg gcctgcatat ggaagaaaaa
    22681 cgcgccatta accgcctgat gaacaaagcg gcgagcagct ctattggcga tgttgagctg
    22741 gtgatttttg tcgttgaagg cacccgctgg acgccggacg acgaaatggt gctcaacaaa
    22801 ctgcgcgaag gcaaagcgcc ggtaatcctc gcggtgaaca aagtggacaa cgtgcaggag
    22861 aaagccgatc tgctgccgca cctgcagttc ctggcaagcc agatgaactt cctcgatatc
    22921 gtgccaatct ctgccgaaac cgggctgaat gttgacacta ttgcggcaat cgtgcgtaag
    22981 catctgcctg aagcgactca tcacttcccg gaagattaca tcaccgatcg ctcacagcgt
    23041 tttatggcgt ctgaaatcat ccgcgaaaaa ctgatgcgtt tccttggcgc tgaactgccg
    23101 tactccgtga ccgtggagat cgaacgtttc gtctctaacg aacgcggcgg ttgtgacatc
    23161 aacggtttga ttctcgttga gcgtgaaggg cagaagaaga tggttattgg caacaaaggg
    23221 gccaagatca aaaccatcgg gattgaagcg cgtaaagaca tgcaggaaat gttcgaagcg
    23281 cctgtccacc ttgaactgtg ggtaaaagtg aaatcgggtt gggccgacga cgaacgcgca
    23341 ctgcgcagtc tcggttatgt tgacgatctt taagagtcac tccgatggaa ggctggcagc
    23401 gcgcatttgt cctgcatagt cgcccgtgga gcgaaaccag cctgatgctg gacgtcttca
    23461 cagaggaatc gggtcgcgtg cgtctggttg ccaaaggcgc acgctctaaa cgctctactc
    23521 tgaaaggtgc attacagcct ttcacccctc tcttgctacg ttttggcggg cgtggcgaag
    23581 tcaaaacgct gcgcagtgct gaagctgtct cactggcgct gccattaagc ggtatcacgc
    23641 tttacagcgg tctgtacatc aacgaacttc tctcccgcgt actggaatac gagacgcgct
    23701 tctctgaact ctttttcgat tacttgcact gcattcagtc tcttgcaggt gacactggta
    23761 cgccagaacc cgcgctgcgt cgctttgaac tggcactact cgggcatctg ggctatggcg
    23821 tcaattttac ccattgcgcg ggtagcggcg agccggtaga tggcaccatg acgtatcgtt
    23881 atcgcgaaga aaaagggttt atcgcaagcg tcgttatcga caataaaacg ttcaccggaa
    23941 ggcagttaaa agcgttaaac gcgcgggaat ttcctgacgc agacacacta cgcgccgcga
    24001 aacgctttac ccgaatggcg cttaagccgt atcttggcgg taaaccatta aagagcaggg
    24061 aactgttccg gcagtttatg cctaagcgaa cggtgaaaac acattatgaa tgatgaggat
    24121 tgtcatggct gaattactgt taggcgtcaa cattgaccat attgcaacgc tgcgtaacgc
    24181 acgcggtact gcttacccgg atccggtgca ggcggcgttt attgccgagc aggcgggagc
    24241 ggacggcatt accgtgcatt tacgtgaaga tcgccgccac attactgacc gcgatgtgcg
    24301 catcctgcgg cagacgctgg atacccgaat gaatctggag atggcagtga ccgaagagat
    24361 gctggcgatc gccgttgaga cgaagccaca tttttgctgc ttggtaccgg aaaagcgtca
    24421 ggaagtaaca accgaaggcg gcctggacgt tgcagggcag cgtgaaaaaa tacgcgatgc
    24481 ctgcaaacgt ctggcagatg ccgggattca ggtttctctg tttatcgacg ccgatgaaga
    24541 gcagatcaaa gctgcggcag aggttggcgc accgtttatc gagatccaca ccggttgcta
    24601 tgctgatgcc aaaactgacg ccgaacaggc gcaagagctg gtgcgtatcg ccaaagccgc
    24661 gacctttgcc gctagtctcg gtctgaaagt taacgccgga cacggtctga cctatcacaa
    24721 cgtgaaagcc attgccgcca tccctgagat gcatgaactg aatatcggtc atgccattat
    24781 tggccgtgca gtgatgaccg gactgaaaga tgcggtggca gaaatgaagc gtctgatgct
    24841 ggaagcgcgt ggctaatggc aatattaggt ttaggcacgg atatcgtaga gatcgctcgc
    24901 atagaagcag tgatcgcccg atccggtgag cgcctggcac gccgcgtatt aagcgataac
    24961 gaatgggcaa tctggaaaac gcaccaccag ccggtgcgtt ttctggcgaa gcgttttgct
    25021 gtgaaagaag ccgcagcaaa agcgtttggc accgggatcc gcaatggtct ggcgtttaat
    25081 caatttgaag tattcaatga tgagctcggc aaaccacggc tacggctatg gggtgaggca
    25141 ttaaaactgg cggaaaagct cggcgttgta aatatgcatg taacgctggc agatgaacgc
    25201 cactatgctt gtgccacggt aattattgaa agttaaaata ataccgttct ctttaaaaag
    25261 taaaaccccc gaactggtaa ggaacggggg ttttactttt taactttaat tttgtgcagc
    25321 atgcaccctc aacaacagcg attatggatc agtgtactta tcttttacaa gcaacttgca
    25381 atctttagca taaaaactcg agcctttacg aagaaagcaa tattgatgga aagattaacg
    25441 tgaccgccaa ttcgtaagta cattaaaatt ggcttcgtta ttgaagattt tgctgtgctt
    25501 tacaccatgc cacagaattc ccccattgaa acgagtggtg tcgtcaaagc tctggtgtgg
    25561 agtgcagcat gcaccctcaa taactcgcac gttcagtttt ggggagatgt aagggctaat
    25621 ctgaatggct gcattccttg tttaaggaaa aacgaatgac tgattgccga tacctgatta
    25681 aacgggtcat caaaatcatc attgctgttt tacagctgat ccttctgttc ttataacaca
    25741 aggaaacgta cttaaggtgc gtccggtgaa ccaatcggac gcgcctttaa taactataaa
    25801 taagtgtctt ggcagatact atataaatta acttagtgaa tgattatgct aatgtcatca
    25861 attaaataaa tataatggcg ttaaggcttc ccagtaatat aattaatact ctacttccag
    25921 agtagaatat taaattttat ccgcgtggtg catcagcaca aatttatccc acaactgttc
    25981 ttctgtctcg acatgcgccg gatctttcac aatagtattg gggatcgggc acaccttctg
    26041 gcaggttggt gtctcgtagt gccctacgca ttcggtacac ttatcgctgt taatttcgta
    26101 gatatgatct cccattgaaa tcgcctcatt cgggcattcg ggttcacaca tatcacaatt
    26161 aatacagcgt ttagtaatta gtaaagacat ttcaatggat taccgttaaa tcattttaaa
    26221 atcagtaagt tgtatcgagt ttgtatgctt tactgtcatt aacttactgt atgttgatcc
    26281 agtgtattta accttgataa actcagtcca gcaacacaaa accgcaacac attgcatttt
    26341 gtcccgtaga aaagacttgt atgtgtgagc ttgttttctg cgcctacgca gataaggatt
    26401 gagaatgccg cgcactgtaa cacataatcc ggatagcccc aataatgacg atgttttagc
    26461 cgcatctgaa aaatgggacg cctgtaaacc cccctatacc agcgcacaca tgaaaatctg
    26521 tgttgctgcc gccaaaatca tcctcgctgc ttccggcgtg gctcgctgtt ccaaatacga
    26581 aaaagagaac tatctccgta tcgatttcag caaagccggt aaggttacat tttacgccga
    26641 gtttccaaaa aagatgggcc tcaaaggtaa aaagctcggc gagtggccgg agctcgttat
    26701 ccagctggcg cgcgaaaaag cactaggtat ggctgacggt ggactgcggg cagagtccgt
    26761 acatgcagcg ctggaaatgt accgggatga cccaaagcca aagtagcccg gcagaagctg
    26821 agcccggaca gtttcacaac ctacggggtg cgtatcgacc ggattaaagt aacgttcggc
    26881 gagcgtgagg tgttcagcga cgtaacatac aatcggctgg tggaagtgct ggacgagtgg
    26941 atcgccactc gctcgaacaa taacgccctg gagttgtttg ccgagctccg tcggttctgg
    27001 aagttctgcg cacctactct ttgcaacggc cgcaatgttg ccgccagtct gccagatgat
    27061 tatgtttcct cccgcgtaca gaaacctacc cccacacggc tttttaccga tattgaatca
    27121 atcgcccgac tctggctcaa tgttgctgcc tgcacctctg tacaccagaa gaatgctgtt
    27181 cgcttcatga tcatcactgg tgttcgtccg attaatgtcc ataacctgcg ctgggactac
    27241 gtttacgagg aggctggtga aattgtttat ccggaagggg ttatcggcat gcgaggggct
    27301 atgaaaacac aaaaggcttt ccgcctgccg ataacgcctg agatccggcg gattatcgac
    27361 gagcagaaag cctggcgtga
//
LOCUS       DAJTUI010000053        26491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTUI010000053 DAJTUI010000000
VERSION     DAJTUI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.053"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            336..629
                     /locus_tag="OO464_002368"
     CDS             336..629
                     /locus_tag="OO464_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR6227494.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            701..1360
                     /locus_tag="OO464_002369"
     CDS             701..1360
                     /locus_tag="OO464_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6227495.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1452..1898
                     /locus_tag="OO464_002370"
     CDS             1452..1898
                     /locus_tag="OO464_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR6227496.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2105..3005)
                     /gene="hlyE"
                     /locus_tag="OO464_002371"
                     /pseudo
     CDS             complement(2105..3005)
                     /gene="hlyE"
                     /locus_tag="OO464_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3378..3797
                     /locus_tag="OO464_002372"
     CDS             3378..3797
                     /locus_tag="OO464_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6227497.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3797..5065
                     /gene="umuC"
                     /locus_tag="OO464_002373"
     CDS             3797..5065
                     /gene="umuC"
                     /locus_tag="OO464_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6227498.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5111..5641)
                     /gene="dsbB"
                     /locus_tag="OO464_002374"
     CDS             complement(5111..5641)
                     /gene="dsbB"
                     /locus_tag="OO464_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6227499.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5787..7328)
                     /gene="nhaB"
                     /locus_tag="OO464_002375"
     CDS             complement(5787..7328)
                     /gene="nhaB"
                     /locus_tag="OO464_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6227500.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7549..8268
                     /gene="fadR"
                     /locus_tag="OO464_002376"
     CDS             7549..8268
                     /gene="fadR"
                     /locus_tag="OO464_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6227501.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8320..9852)
                     /locus_tag="OO464_002377"
     CDS             complement(8320..9852)
                     /locus_tag="OO464_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6227502.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10182..11480
                     /gene="dadA"
                     /locus_tag="OO464_002378"
     CDS             10182..11480
                     /gene="dadA"
                     /locus_tag="OO464_002378"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6227503.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11490..12560
                     /gene="dadX"
                     /locus_tag="OO464_002379"
     CDS             11490..12560
                     /gene="dadX"
                     /locus_tag="OO464_002379"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR6227504.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12728..14142)
                     /locus_tag="OO464_002380"
                     /pseudo
     CDS             complement(12728..14142)
                     /locus_tag="OO464_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14753..16489)
                     /locus_tag="OO464_002381"
     CDS             complement(14753..16489)
                     /locus_tag="OO464_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6227505.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16584..17498)
                     /gene="ldcA"
                     /locus_tag="OO464_002382"
     CDS             complement(16584..17498)
                     /gene="ldcA"
                     /locus_tag="OO464_002382"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6227506.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17598..18209
                     /gene="emtA"
                     /locus_tag="OO464_002383"
     CDS             17598..18209
                     /gene="emtA"
                     /locus_tag="OO464_002383"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6227507.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18211..18945)
                     /locus_tag="OO464_002384"
     CDS             complement(18211..18945)
                     /locus_tag="OO464_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6227508.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19146..19400
                     /locus_tag="OO464_002385"
     CDS             19146..19400
                     /locus_tag="OO464_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6227509.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19450..20910)
                     /locus_tag="OO464_002386"
     CDS             complement(19450..20910)
                     /locus_tag="OO464_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6227510.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20847..21404)
                     /locus_tag="OO464_002387"
     CDS             complement(20847..21404)
                     /locus_tag="OO464_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR6227511.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21430..22284)
                     /locus_tag="OO464_002388"
     CDS             complement(21430..22284)
                     /locus_tag="OO464_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR6227512.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22284..23093)
                     /locus_tag="OO464_002389"
     CDS             complement(22284..23093)
                     /locus_tag="OO464_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR6227513.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23103..23861)
                     /locus_tag="OO464_002390"
     CDS             complement(23103..23861)
                     /locus_tag="OO464_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6227514.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23858..24838)
                     /locus_tag="OO464_002391"
     CDS             complement(23858..24838)
                     /locus_tag="OO464_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR6227515.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24838..25860)
                     /locus_tag="OO464_002392"
     CDS             complement(24838..25860)
                     /locus_tag="OO464_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR6227516.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6656 a         6457 c         6835 g         6543 t
ORIGIN      
        1 tgaactcaac attgacattc ttacccgcag caaagctcgt ggaggttagc agtgcaagca
       61 gcaatacagc cttactgatg ctcttgattt tcattggtag tctcctgttc agaatgagta
      121 cgatgaactg ttataatata acaatccctt acgggagaat tccagtggct tttctgcgtt
      181 cggtaaaatc ccggcgcggc attttttgcc ggggggatta cttataaagg ggcatggact
      241 caacaaaccg cagaaatcta cagctggctg gcttgtggcg cgggtttcat tgatgttagc
      301 ttatgccaaa acctggcatc ttaaagagta aaagtatgtt ttgtgcgatt tatcgaagca
      361 gcaagcgtga ccagacctat ttatatgtcg aaaaaaaaga cgatttttcg cgtgttcctg
      421 aggaactgat gaaaggtttt ggtcagcctc agttagcgat gattctgccg ttggatgggc
      481 ggaagaaact ggtcaatgct gatattgaaa aggttaaact ggcattaacc gagcaaggtt
      541 actatttgca gttaccgcca ccacccgaag atttgctgaa gcaacatctt tccgtcatgg
      601 ggcagaaaac agacgacact aacaaataac cgatatccgg cggtggcatt atctttgtcg
      661 gcgcgggttt tcatatccac gataaggtga ggggaacgtt atgtatcaac atcacaactg
      721 gcaaggtgcg ctgctggatt atccggtgag taaagtagtc tgtgttggca gtaactatgc
      781 caaacatatt aaagagatgg gcagcgcagt gcccgaagag ccagtgctgt ttattaaacc
      841 agaaacggca ctgtgcgatc tgcggcagcc attggcgatc ccatccgatt tcggttcagt
      901 tcatcatgaa gtcgaactgg cggtgttgat tggcgcgacg ctgcgtcagg caacggaaga
      961 gcatgtccgc aaagccattg ccggttacgg cgtggcgctt gatctgacat tgcgtgatgt
     1021 tcagggaaaa atgaagaaag ccgggcagcc gtgggaaaag gctaaagcgt ttgataactc
     1081 ttgtccgctt tccgggttta tccccgcggc ggaatttacc ggcgatccac aaaatacaac
     1141 gctgggcctg agcgtaaacg gcgaaaaacg ccagcaaggt acgactgcgg acatgatcca
     1201 taaaatcgtt ccgttgatcg cttatatgag caagtttttt accctcaagg ccggtgacgt
     1261 tgtgttgaca ggcacgcctg atggcgtcgg cccgttgcaa agcggtgatg agctgacagt
     1321 cactttcgat gggcattctt tgacaactcg cgttttgtaa tactttttgc cgccagaaag
     1381 cggcggcaaa acttgcatcg ctgtgccaga ctggttataa ggtgcgtttt aacgtaatgg
     1441 cggaacacct gatgagcgat gtacctttct ggcaaagtaa aaccctggac gaaatgagcg
     1501 atgcggaatg ggagtcgttg tgtgatggtt gcggtcagtg ttgcttgcat aaactgatgg
     1561 atgaagacac cgacgaaatc tacttcacta acgtcgcctg tcgccagctc aatattaaaa
     1621 cctgtcaatg tcggaactac gaacgtcgtt tcgagtttga acccgactgc attaaattaa
     1681 cccgtgaaaa tctgccaaca ttcgaatggc tgccaatgac ctgcgcttat cggttgctgg
     1741 cggaaggtaa agatttacct gcgtggcatc cgctacttac tggttcgaaa gcggcaatgc
     1801 atggtgaacg tatctctgtg cgtcatatcg cagtgaaaga atcagaagtg attgactggc
     1861 aggatcatat cttaaataaa cctgactggg cgcagtgata aatttaatag atgctgatgg
     1921 gggctttctt aatatacgtc tggatgctcg ccatttcgta gccctgtttt tgaaagcgct
     1981 ctgtcataat gagagttacc cgatacctta ttatcaacag gtaactctca atctttaaaa
     2041 aacgtctgtg tttttaaatg tgataaacct tataccttga gtacatggag agaataatcg
     2101 cttatcagac ttcaggtact tcaaagagtg tctttttacc gtgtcttttc tgatactcat
     2161 tacaggtgtt aatcattttt ttggccgctg cttttagcaa agaaagcatt aaatcatcat
     2221 aatcaacata gaatctggtt gtttcagttt ccgtttttat ctccccgatg gcggctattt
     2281 cggtggttaa tttcaatttg gcggcatcga tatctttatt cgcttgttta accgtgttag
     2341 acagggtggt aaagaaactc tgcacagatt ttaacttgtt cttcaattct ggaatcagtt
     2401 tcccttcaac tacgcccgca gcaatagaat aggaaatgat taaaccaaat ggaccggcga
     2461 cgacatcggc tgcggcaccg gcatacgctt ccttcctgat tttatctacc tgtgactgga
     2521 aatagctgct tttttctgaa aaatcattgg ttaactggct atctaacgcc agcagtttcc
     2581 cggaagcgtt gttgaaactt tgtgagctta ccagcaggga tttttgcgct tcattcagct
     2641 tcgtgatgcc gtcatccagt accttaatga ggggcggatg ctttcttctc attgtactca
     2701 tcaaatagca aaatatacgc tgcgagcaat tgcgtcgcaa caccacacca ttcatacact
     2761 gtttgggttg cttcaaaata cttatcctgg ctatccataa gtaaggtttt aatatcgccg
     2821 actaaaacgg aggctgcctg tgaatactcc tgtttaaagc gacttaactc ttttatggtt
     2881 tcatcaaagg tctgccaggg gatgacctga tcgagatatt tattataaag atctaatgct
     2941 ccatctgcgg tttcgattgc gtttttaact acttctaccg ttttatctgc aacgatttca
     3001 gtcataatca ttcgcctctt taaatatata aattgtaatg aaactcctgt tttacaacta
     3061 ttaataaatt ttacttcatc taattcatag ttagccgggc gggatgcgtc aatgtcttta
     3121 tttctattaa tatgataaat atcaaacaat gtttaatgtc attatggcga atgcttctat
     3181 tctatttttt tgccgggtga tatttttcat ttctgctgga tgagcgtcgt cgccagaagg
     3241 ccacgtgagc acaggataag agaacgaaaa atcagcagtc tatgcagcga caaatattga
     3301 tagcctgaat cagtattgat ctgctggcaa gaacagacca ctgtatataa aaacagtata
     3361 acttcaggca gattattatg ttgtttatca agcctgcgga tctccgcgaa attgtgactt
     3421 ttccgctatt tagcgatctt gttcagtgtg gctttccttc accggcagca gattacgttg
     3481 aacagcgcat cgatctgaat caactgttga tccagcatcc cagcgcgact tacttcgtca
     3541 aagcaagtgg tgattctatg attgatggtg gaattagtga cggtgattta ctgattgtcg
     3601 atagtgctat taccgccagc catggtgata ttgtcatcgc tgctgttgac ggcgagttta
     3661 cggtgaaaaa attgcaacta cgcccgacgg tacagcttat tcccatgaac agtgcgtact
     3721 cgcccattac catcagtagc gaagatacgc tggatgtctt tggtgtggtg atccacgtcg
     3781 ttaaggcgat gcgctgatgt ttgccctctg tgatgtaaac gcgttttatg ccagctgtga
     3841 gacggtgttt cgccctgatt tatggggtaa accggtggtt gtgctatcga ataatgacgg
     3901 ttgcgttatc gcccgaaacg ctgaggcaaa ggcgctgggc gttaaaatgg gcgatccctg
     3961 gttcaaacaa aaagatctgt ttcgtcgctg tggtgtggtt tgctttagca gcaattatga
     4021 gctttacgca gacatgagca atcgggtgat gtcgacgctg gaagaactat cgccccgcgt
     4081 cgagatttac agtattgatg aagcattctg cgatctgaca ggtgtgcgta attgtcgcga
     4141 tctgactgat tttggcagag aaattcgcgc aacggtgcta caacgtaccc atcttactgt
     4201 tggtgtgggg atcgcccaga ccaaaacgtt ggctaagctt gccaatcatg cggcaaaaaa
     4261 atggcagcgg cagacgggtg gggtggtgga tttatcaaat ctggaacgcc agcgtaaatt
     4321 aatgtctgct ctccccgtgg atgacgtctg ggggattgga cggcggatca gcaaaaaact
     4381 ggacgcgatg gggatcaaaa ccgttctcga tttggcggat acagatatcc ggtttatccg
     4441 taaacatttt aatgtcatgc tcgaaagaac ggtgcgtgaa ctgcgcggcg aaccctgttt
     4501 gcaactggaa gagtttgcac cgacgaagca ggaaattatc tgttcccgct cgtttggtga
     4561 acgcatcacg gattatacgt cgatgcggca ggccatttgt agttacgctg cccgggcggc
     4621 ggaaaaactt cgcagtgagc atcaatattg tcggtttatc tccacattta ttaagacgtc
     4681 accatttgcg ctcaatgaac cttattacgg caatagcgcg tcggtaaaac tgctgacgcc
     4741 cactcaggac agcagggata tcattaacgc tgctacgcga tctctggatg ccatctggca
     4801 agcgggccat cgctaccaaa aagcgggcgt gatgctgggg gatttcttca gtcagggagt
     4861 cgcgcagctc aatttattcg atgacaacgc accgcgcccc gggagtgagc aattgatggc
     4921 ggtaatggat actctgaatg ctaaagaggg cagaggaaca ctctattttg ccgggcaggg
     4981 gatccaacaa caatggcaga tgaagcgagc tatgctttca ccacgttata caacgcgaag
     5041 ttctgattta ctgcgggtca aataaatata gcggcaggaa aaaagcgctc ccgcaggagc
     5101 gccgaatgga ttagcgaccg aacagatcac gttttttcgc tttaaacggc tgggaaatca
     5161 ccaccagcac cgcgacaatc aggtaagcga taaaaatacc gagcagccac tgcggcattt
     5221 ccatacctaa aaaatcccac tgacgctcgg cgcaatcgcc agaggcgaca aacacttgcg
     5281 gcacccattt atccagcggc agccattccg ggaaacgaac cataaaatca caggtggcaa
     5341 atggcgaagg atagagctga agcatggtgt gctcgtaagt taactgcaca ccgcggaacg
     5401 cactatacaa ccagataacc atcgctacat aacgcagcgg agttttcggg gcgatcgcgc
     5461 caatcagcgc agcacccaga acgccgaata acgcgcagcg ttcataaata cagagcacgc
     5521 aaggtttcag taacatcaca tgctggaacc acagcgccgt cagttccagt gccagagcag
     5581 taaacgccat caacagccac gcgccccggc cttgtgaaca ttggttcaaa aatcgcaaca
     5641 taatcatttc cctgcaatat gcatagagtg cgcagtttaa accaattcat tcgctgcgcc
     5701 accaggaggc agcaaaaata ggacgcaatt ggacaattat cgggcgaaaa ggcaaaccgg
     5761 gcatttatat gcccggtaag ttgttatcaa agcgttgcta tccagcccat ttgcataaac
     5821 cattcggtta caggggcaag cgtaaactcg acgcagagca agccgacgag tgtcagtacg
     5881 agggtgtaag gcagggccat ccacaccatg cggccataag agaggcgaat caatggcgcg
     5941 agtgcagagg tcagcaggaa caggaacgca gcctgaccgt tcggcgtagc gacagagggc
     6001 agattggtac cggtattaat ggcgaccgcc agcagctcgt attgcttcaa cgtgatagcg
     6061 ccactttcca ttgccgcttt cgcttcgttg atataaatcg tccccacgaa gacgttatcc
     6121 gaaatggatg acagcagacc gttgaaaata tagaacagcg acagctgagc atgttcagat
     6181 gcctgcaaca caaactgaat aatcggcgaa aacagttgtt ggtcgataat caccgcgact
     6241 accgagaaaa acaccgtcaa cagtgcggtg aatggcagag attcggtgaa ggctttaccg
     6301 atagcatgtt catcggtgac accggtcaat gatgttgcca gaataatgac tgacaaacca
     6361 atcaagccaa cttccgccag atgcaacgcc agcgcagtca ccagccagac gccaataatc
     6421 gcctggacaa tcagacggat tttatcctga cgggtacgct ggtggcggct ttgatcgtca
     6481 aactgttgca acacttcgcg gactttctcc ggcagcgttt caccgtagcc aaaccaacgc
     6541 agcttctcta ccagcaggca ggttaacagg ccacaaatca gaaccggaac ggtcaccggc
     6601 gacatgcgca ggaagaaatc gccaaaatgc cagccagccg ctttagcgat gatcaggttc
     6661 tgtggttcgc ccaccatggt cattacgccg cctaatgcgg tgccgacacc ggcatgcatc
     6721 atcaggctac gcagaaagcc acggaactgt tccagaacca cgttgtaatg cttgtcgata
     6781 tgactatcgt cttgcaggtc ggtgtcttcg gtacgggaag aggctacgcg atgataaata
     6841 ccataaaaac cgactgcaac gctgatcacc accgccacga cggttaaggc atcgaggaac
     6901 gcggagagga acgctgccgc cacgcaaaaa gagagcgaca gcagcatttt ggagcgaatg
     6961 cttaacagca aacgggtaaa tatgaacagc aacagctgtt tcataaaata gatacccgcc
     7021 accataaaca tcaataacag caagacttca agatttgccg ccacctcttc acggacgtgt
     7081 tccgcgctgg tcatgccgat gaataccgct tcgatagcca acagaccacc ggggagtagc
     7141 gggtagcatt tcagggccat cgccagagtg aaaataaatt ccgctaccag caaccagccc
     7201 gcgacgaaag ggctgatgag gaaaattaac gggtttacga ttaagaaaat gatgagggcg
     7261 agtttgtacc agtcggggga ctggcccaaa aaattgcgcc atagcgcgcg gccccaggag
     7321 atctccatga tggtttccct taccttacaa ataatcaatg atgtttttat gtttaaacgc
     7381 aaagcttaac ggtcaggcag gagtgaggca agtcttgata gtcaagggga aagagatgcg
     7441 gaaaatgaag ccttgatccc tttttcttct ttttgtctgc tatcagcgta gttagccctc
     7501 tggtatgatg agtccaactt tgttttgctg tgttatggaa atctcactat ggtcattaag
     7561 gcgcaaagcc cggcgggttt cgcggaagag tacattattg aaagtatctg gaataaccgc
     7621 ttccctcccg ggactatttt gcccgcagaa cgtgaacttt cagaattaat tggcgtaacg
     7681 cgtactacgt tacgtgaagt gttacagcgt ctggcacgag atggctggtt gaccattcaa
     7741 catggcaagc cgacgaaggt gaataatttc tgggaaactt ccggtttaaa tatccttgaa
     7801 acactggcgc gactggatca cgaaagtgtg ccgcagctta ttgataattt gctgtcggtg
     7861 cgtaccaata tttccactat ttttattcgc accgcgtttc gtcagcatcc cgataaagcg
     7921 caggaagtgc tggctaccgc taatgaagtg gccgatcacg ccgatgcctt tgccgagctg
     7981 gattacaaca tattccgcgg cctggcgttt gcttccggca acccgattta cggtctgatc
     8041 cttaacggga tgaaagggct gtatacgcgc attggtcgtc actatttcgc caatccggaa
     8101 gcgcgcagtc tggcgctggg cttctaccac aaactgtcgg cgttgtgcag tgaaggcgcg
     8161 cacgatcagg tgtacgaaac agtgcgtcgc tatgggcatg agagtggcga gatttggcac
     8221 cggatgcaga aaaatctgcc gggtgattta gccattcagg ggcgataatc ccttccgttt
     8281 aaagagcaac cccctcaaac gaggggtttt ttgttgtttt tacagatttc ccattcttgg
     8341 cgggcaacgt tccagcaact cgatgctgcc gtcttcgttt tgctgttcga gcatcacatc
     8401 aaatccccac aggcgatgca catgcttcag gacttctttg cgcccccgat ccagcggtgc
     8461 gcgattatgt ggaatataac gcagcgtcag cgaacggtcg ccgcgcaaat ccacgttcca
     8521 gatctgaata ttcggctcca gattacttaa gttatattgc gacgataacc ggttacggat
     8581 ctcccgataa ccttcttcat tatgaatagc ggaaatctcc agataattat gccgatcatc
     8641 atccagcacg gtgaagaagc ggaaatcacg catcactttc ggtgacagaa actggctgat
     8701 aaaactctca tctttgaaat cacgcatcgc gaaatgcagc gtttccagcc agtcggaacc
     8761 ggcgatatcc gggaaccagt atttgtcttc ttccgttggc gactgacaaa tccgtttaat
     8821 atcctggaac atggcgaacc cgagggcata cgggttgatg ccgctgtacc acgggctgtt
     8881 ataggggggc tggaagacca cattggtgtg gctgtgcaaa aactccagca taaaacgttc
     8941 cgttactttc ccttcatcat acagatggtt aaggatggta tagtgccaga aggtcgcaca
     9001 gccttcgttc atcacctgag tctgtttttg cggataaaaa tactggctca ccttacgcac
     9061 aatacgcagg atttcacgct gccatgattc cagcagtggc gcatttttct ccataaaata
     9121 gagcaggttt tcttgtggtt cggacggata gcggcgcgct tcagcaaccg ttttctcttc
     9181 ctcgcgcttc ggcagggtac gccagagcat attgacctga ctttgtagat actcttcgcg
     9241 acttttctgc cgggctttct cttcttgcag cgagattttt tgcgggcgtt tgtaccggtc
     9301 cacgccgtag ttcatcagcg cgtggcacga gtccagaagc cgttctactt catcaacgcc
     9361 ataacgttct tcgcactcgg taatatattt acgggcaaaa atcagataat cgacaatcga
     9421 actggcgtcg gtccaactgc ggaataagca attgttttta aagaaagagt tatgtccata
     9481 gcaggcgtgt gccatcacca gcgcctgcat agtaatggtg ttctcttcca tcaagtaagc
     9541 gatacacggg ttagagttaa tgacgatttc ataggccagc ccttgctgac cgtgcttata
     9601 cagccgttca gtctcgataa actttttacc gaatgaccag tgcggatagt taattggcat
     9661 gccgacgctg gaataggcat ccatcatctg ttctgaggtt atcacttcaa tctggtgcgg
     9721 gtaggtatcc agccggtaga gtttcgccac ccggtctatc tctgccagat aaacatccag
     9781 caggtcgaac gtccagtcgg gtccatcgct caaacgtgtg gtgtccttat tcatagaatc
     9841 gatcgtcgcc atacgcgcac ctcattgttg tcggcgctct ctgtgtggag cacctcattt
     9901 caagcataga acacctgtta aaaaccgcgt cgccggagaa tttttttctt tgcgatttct
     9961 tattatcaga gtgccactaa tccgcttctg aacggaattt tatgctggat aaaaagggcg
    10021 ttcagcagga gatactaaag acgccatatt gccgcagagt cagggagatg tgagtcagct
    10081 caccataaaa aagccgtatg ttgaataata ttttcaactg agttatcaag atgtaattag
    10141 attattattc ttttactgta tctaccgtta tcggagtggc tatgcgagtt gtcatactgg
    10201 gaagtggtgt ggtaggcgtt gccagcgcct ggtacttaaa tcaggcagga catgaggtca
    10261 ccgtcattga tcgggagccg ggggccgccc tggaaaccag tgccgcgaat gccgggcaaa
    10321 tctcccccgg atatgctgca ccgtgggcag caccaggtgt gcctttaaaa gcgattaaat
    10381 ggatgttcca gcgccatgcg ccgctggcgg ttcgtctcga cggtacgcag tttcagttga
    10441 aatggatgtg gcaaatgtta cgtaactgcg acaccagcca ctacatggaa aacaaagggc
    10501 ggatggtgcg tctggcggaa tacagccgtg attgcctgaa agcattgcgc gccgaaacca
    10561 atattcagta tgaagggcgt cagggtggga cgctgcaact gttccgtacc gaacaacagt
    10621 atgaaaatgc gacccgcgat atcgccgtgc tggaagatgc cggcgtaccg tatcagctgc
    10681 tggaatccag ccgcctggcg gaagtggatc ccgcgctggc agaagtagcg cacaaactga
    10741 cgggcggcct gcagttaccc aatgatgaaa ccggagactg ccagctgttt acccagaatc
    10801 tggcgcggat ggcagagcag gcgggggtta aattccgctt taatacgccc gttgaccaac
    10861 tgctttgcga cggcgagcaa atctacggcg tgaagtttgg cgatgaagtg attaaggccg
    10921 atgcgtatgt gatggcgttt ggttcttact cgacggcgat gctcaaaggc attgttgata
    10981 ttccggtgta tccgctgaaa ggctactcgc tgactattcc aattgcgcag gaagatggtg
    11041 cgccagtatc caccattctt gatgaaacct acaaaatcgc cattacccgt ttcgataacc
    11101 gcattcgtgt tggcggaatg gcggaaattg ttggttttaa caccgagctg ttgcaaccgc
    11161 gtcgtgaaac gctggaaatg gtggttcgcg atctctatcc acgcggcggt catgtcgagc
    11221 aggcgacttt ctggacgggt ttgcgcccga tgacgccaga cggtacgccg gttgtcggga
    11281 gtacacgctt taaaaatctg tggctgaata ccggtcacgg cacgctcggc tggacgatgg
    11341 cttgcggttc cggtcagttg ttaagcgatc tgctctctgg tcgcacgcca gcgatcccat
    11401 atgaggatct aagcgtagcg cgctacagcc gtggatttac gccatcacgt ccgggccatt
    11461 tacatggcgc acacagctaa ggaaacgaga tgacccgtcc gatacaggcc agcctcgatc
    11521 tgcaggcatt aaaacagaat ctgtccattg tccgccaggc cgcgccgcac gcgcgcgtct
    11581 ggtcggtggt aaaagcgaac gcttacgggc atggtattga gcgtatctgg agcgcgctcg
    11641 gggccaccga tggctttgca ttacttaacc tggaagaggc aataacgtta cgtgagcgcg
    11701 gctggaaggg gccgatcctg atgctggaag gatttttcca tgctcaggat ctggagattt
    11761 atgaccagca ccgcctgacc acctgcgtac acagcaactg gcagctcaaa gcactgcaaa
    11821 atgcgcggct aaaagcaccg ttggatattt atcttaaagt gaacagtggg atgaatcggt
    11881 tgggcttcca gtccgatcgc gtgcttaccg tctggcagca gttgcgggca atggcgaatg
    11941 ttggcgaaat gaccctgatg tcgcattttg ctgaagcgga acatcctgat ggaatttccg
    12001 gcgcgatggc gcgtattgag caggcggcgg aggggctgga gtgtcggcgt tcgttgtcca
    12061 attcggcggc gactctgtgg cacccggaag cgcattttga ctgggttcgg cctggcatta
    12121 ttttgtatgg cgcttcgccg tccggtcagt ggcgtgatat cgccaatacc ggattacgtc
    12181 cggtgatgac gctaagcagt gagattattg gtgtccagac gctaaaagcg ggcgagcgtg
    12241 tgggctacgg cggtcgctat actgcgcgcg atgaacagcg aatcggcatt gtcgccgcag
    12301 gatacgccga cggttatccg cgccatgcgc ctaccggtgc tcctgtttta gtggacggcg
    12361 tgcgcaccat gacggtgggg accgtctcga tggatatgct ggcggttgat ttaacgcctt
    12421 gcccgcaggc ggggattggt acgccggttg agctgtgggg caaggagatc aaaattgatg
    12481 atgtcgccgc cgctgccgga acggtgggct atgagttgat gtgcgcactg gcgttacgcg
    12541 tcccggttgt gacagtgtaa cttgttgtaa gccggatcgg aggcagcgtc ttctggttgc
    12601 gaaaaaatca tccatccggc tggtcagcaa ctgtagttgt taatgtgaca gagccattgc
    12661 ccatgatagt gtccattaaa agaatggaca ctatttcccc ggaacctgaa ctcgccgcac
    12721 aggcgttcta cataaaacgc ttacgcttca ttgttgactc ggagtcgact tcagataaat
    12781 cacgcttcac ccttgatgca gattcgggtt catgttcaga ttgttgatta acaggctgcg
    12841 agtcctgttg tctttccaga ctcaacaggc ccaactgttg atccaggagc tccaggtcct
    12901 cctgtacctt cacgcgctcc aggagttccg ggtcatccag gaactccaga tccataaggt
    12961 cctccagctc ctcgagtcct tccaggtcaa cctagccttc cttgtgcaaa aagtccaaaa
    13021 ggtccttcag gccctgctgt tttttcaggc cctcgacggc tttgagcagc gtcggtaaat
    13081 tgtctttgtc ccacgggtta ccattacagt caaacacctt caggctctgg ggctgcggca
    13141 cctgtaccgt tttaaacttg ttttctccac agtagtaaat tattagtttg ggcggcagcc
    13201 gtggtgaaga gatcaaatcg ttactagaac agtcaaatac tttcaggcga gtcaagttct
    13261 ccagcatctc atcaggtatt gagttaatat tgttgccggc acaggatagt ttatacaggt
    13321 actggggcat cgacggcggt aaaaattgca agaagttttc agaacagtta atcactctca
    13381 ggttagtcga cagctcaggc agccagataa tactgttttc atagcacttc agtaccatca
    13441 gttttgaggg ctgcaccttc ggtaacaaga tcaagacgtt atctgtacag tcaagcacca
    13501 gcagccactt tggcatcacc ttcggtaacg cggtcaacac gtttttactg cagtaaaatt
    13561 caatcagatg tggaggcaaa ttctcaggga gtgaagtcaa tcccaggttg ctaagattga
    13621 gcatactccg tcttgtaatg agacattcct tcattctgcg caatgcttct gcccggtttt
    13681 caatccggct tgctggcagg ttttcttcca ggatattttc cttgatttct tccagacttt
    13741 ctttcaggtc ttcttgggga atttctgact gtttgttttg ctggttttct tccaggattt
    13801 cgtacaggtt tttttgcagg ttttcttgca aaccatcctg ctgtatagcc cattcgcacc
    13861 atttggcgac catcatgaac tcgttatgtt gctgattcag acgagtcccc aacgtgatga
    13921 tggtattgtt gtccacctgt atgttcactt tgcccgcgat actgaggtca atgatctgcc
    13981 agggggcatc accccagctg aatttttgcg ggctgagata cttcatgttc tgcaaaattt
    14041 gattcttgag aagagccgcc ctggcgggcg tcgtacgcct gggttaacgg aagatggggg
    14101 aatggcgggg gcgaatgggc agatgtcttt aaagctagcc ataatgctac gtttccttga
    14161 aataaatatc ctctggcagc gccggagacg tgaatgtggt caccgcaaag gagccacagg
    14221 acgtcatttg cgctgatatc actactcatc gttgactttt ttcctggtca gaaatgccgt
    14281 gtcgggtctg gtacatgctg tattacggcc taggtagctc tgacgtatta aagaaaatac
    14341 gagagtgaac gacccggaaa taatcgttat taagcaaagg aataactata agaaatcgct
    14401 catgcatccg gtgctacagg cggatgaaac gcctattgtg tgggctatgt cagtgcggca
    14461 ggttgcgtgg gggaatatgt tcaggcgatg tcaggcaggg gcccctgatg gttgaccggt
    14521 aatgcggggc tggtttttat tacctgttac agtcggaaaa agcccttacg ctgactttct
    14581 tgagccggtg ttttgagttc attagccaga gcctgcaatg gtttgtcatc cataatttaa
    14641 ccttcttctg atgttgggat gagcatatca aaatcaggaa atgacaagtt tattacaggc
    14701 tcaggctgcg ttcagtaaag catcgcatcc ggcgatagat gctgcattaa atttaagatt
    14761 cagcttcctc ttcagccacc cgaacgccaa tcttcaacac ttcattatct tctttctcgg
    14821 ccaccgtcca gatcatcccg gcaaactcta cctggtcacc gacaaccggt gccgcgccta
    14881 gcaactgctg gacaatttca cccagcgttt gctgtttatc acgatattct cgcccgtctt
    14941 ccaggccata tatcagcgca acatcagcat atttggcgct ggcttcgaga atgaagtcac
    15001 caaagaagcg ttgatccagc gcgaccggtg gcgactggct gaacagttta ccgagcgccg
    15061 ggagatcgcg ttcccgacca attacacaca atacatcgcc ttcgcgcagt cgggtgctgc
    15121 cggtaggatg aagcaactgg ttatcacgaa acagggctgc aatacgcgtc tcttttggca
    15181 tatgcaaatc gcgcagtgcc gcacctacac accatttatc agcactcaat tgataaacaa
    15241 actgctccca cggattttcc ggatgaatat ccaggccaac gcgtgacacc ggacgtccga
    15301 cgggcggaac caccactttg gcttttttcg ccgcccacga gagtgatgtt ccctgcaaga
    15361 gcagtgaaac cagaaccaca aagaaggcga cattaaagaa cagtcgcgca ttctccagcc
    15421 ccgccatcat cgggaacacg gctaggatga ttggcaccgc gccgcgtaat cccacccagc
    15481 tgataaacac gcgctcacgc agattgaaac cgcggaaagg gagcaatcca gcaaataccg
    15541 aaaggggacg ggcgaagaat atcatccatg ccgacaaaat gagcgccgga atggcaatag
    15601 gcagcagatc gcttgggtta accaatagcc ccagcaccag gaacatggcg atttgcgcca
    15661 gccaggcgag gccgtcgaaa ttttgcagga tgccgtagcg gttgcgaatc ggccgattac
    15721 ccagcagaaa accgcacaga taaacagcca gaataccgct gccttccagc gccgtggtta
    15781 aagcaaaaat taaaataccg ccgcttaatg ccagcaatgg atataatccg gcaggcaggg
    15841 cgatgcgatt aatcatttgc agcagtaaat aaccaccgcc aagaccgatg acaatcccga
    15901 ggccaaattg ctgcagaata tcgaccacga acatccaact gacactgctt tcatgctgct
    15961 ggatcattgc aatcagggta atcgtcagaa agaccgccat tggatcatta ctgccggatt
    16021 caatttccag cgtcgagcca acacgttcgt taagcccctt accacccagc agagaaaaga
    16081 ccgctgcagc atcggtggag ccgacgatag cgccgattaa taagccttca atcaaatcaa
    16141 gattaaacag ccacgccgcc atcatgccgg ttaaaccaga ggtgataagt acgcccagcg
    16201 tcgccagcga cagcgccggt cctaacgcca cacgaaagga actggcctga gtgcgcatcc
    16261 cgccgtcgag caaaataatc gccagcgcca ggttacttac catgtaggcg aaggggtaat
    16321 tatcaaacgg gataccgccg acgccatcga ctcccgccag catgccgatc gccagaaaaa
    16381 taaccagaat aggaatgcca agacgggaag aaaatgaact aagtaatata ctgctggtga
    16441 caaggatgga tcctaagatg aaaaggctaa ttattgttgt ggcatccaat ggtcggttac
    16501 tcctgattac gctgtctctt atataaaccc taccatatta gcggcagaga cagcgtttta
    16561 cttagccctg aagcgtgatt tttttacatt ttaagaacag gatgaccgct aatcgtcaac
    16621 tgagcgcctt cccgggtgtt attcagaatg gcatgtgcgc ccaacggcag cgtaaccgtg
    16681 cgttgttcat gaccaaaatc gaggccggta atgagtggaa tcgacaggcg ggaacgcaaa
    16741 aacgcgtaca ctgactccag gttgtaacct gcgtcataat cattgggcgt gctgccgcta
    16801 aagctaccga gaataatcgc cttctgacgt ggcaaaattc ccgcatgata gagctgcaac
    16861 agcatacgtt cgacgcggaa agggtgcacg ttaatatctt ccagcaccag aataccgttc
    16921 tcaatttttg gcatccacgg tgtaccaatc agtgaaatca gcatcgcaag attgcctccc
    16981 cacaacgtgc cttcggtctg acatgtcggg ccttcccctt gccattcaat ggtgaaggtt
    17041 tcattgcgta acgccagcca gaaatggtgc tcggtaaagg cgttcagttc atccgcacca
    17101 aagtttgcta ccagcatggg gccgctaaag gtgatgacat tgccttgcgc cagaagaccg
    17161 cactgaatgg cggtaaaatc gctatgtccg caaatgagta acgggtcatg ttgttggcga
    17221 gccaccagtg cctgccagtc aatatctgcc agtaaacgac tggcaccgta accgccgcgt
    17281 acagccagca cgatggtgtt gggggtagtc agtctggcaa gggaattgag atcttccaga
    17341 cgctccgttt ccgtacccgc aaaacgctca caacgacggg caataacctc gacgttattg
    17401 acttgatgcc ccgcgtcggt aaggcgctgg ataccacgca gcgcggcgtg ctgtttaatg
    17461 ctgtaacccg atggggcaat taagtgaaac agagacatgg caattccttg ctgacaacag
    17521 aaacgaaatg tatatcatgc cgcttaggtg tgccgttgtc acctcaacgg cgattccagg
    17581 ctataaggat agaagaagtg aaattgagat ggtttgcctt tttgattgtg ttattagcgg
    17641 gttgttcatc aaagcatgac tatacgaacc cgccgtggaa cgcgaaagtt ccggtgcaac
    17701 gtgcgatgca gtggatgcca ataagccaga aagccggtgc agcctggggc gtcgatccac
    17761 aattgatcac ggcgattatc gctatcgaat cgggtggtaa tccgaacgcg gtgagtaaat
    17821 cgaatgccat tggtttgatg cagttaaaag cttcaacctc cggacgtgat gtttatcgcc
    17881 gtatgggctg gagtggtgag ccgacgacca gcgagctgaa aaatccagag cgtaatattt
    17941 caatgggggc ggcttacctg aatattctgg aaaccggccc gctggcaggc attgaagatc
    18001 cgaaggtact gcaatatgcg ctggtggtgt catacgctaa cggggcaggt gcgctgctac
    18061 ggactttctc gtcagatcgg aaaaaggcga tcagcaaaat caacgatttg gatgctgacg
    18121 agttcctcga tcacgtagcg cgaaatcatc ctgcgccgca ggctccgcgc tatatctaca
    18181 aacttgagca ggcactggac gcgatgtaaa tcagtcgcgc actttgtccg ctttttcccg
    18241 ggcttctcgc tcgagagaga aaataatccg ctgtaattgc cgctccaccg tcgggctgac
    18301 gttaagaaaa cggaagctca gacggggagt ggtgatggtt tcattcttgc catcaatcac
    18361 tttgcgctcg ctggtggaga ttaactgggc gtcaaagtga aaaacacccc attgccccat
    18421 gttgacttca atttgagcga agcgcatacc ttcttgtaat tcggcaggct ttgctgtttc
    18481 cagtaatgcg cccatgccgc ctaacgacaa atcatacaag cggaaacgta acgtactgtt
    18541 atccgccagt ttggtctggc aaaaataagg cggatggagt ggggcggaga tgcggaaata
    18601 tcggcgtcgt tgtacaaacc ataaggtggg aggcggtacg gtaataaatg ccggaagctg
    18661 caagtattca ctctgctgta gttgttcaac agtaaactcg actttcgcac cctgagtttc
    18721 ggcggtaatg gtaatgtgct gtgcctttag cacggcgatg ttgtcttcgg cttgactgcc
    18781 gaaatccagc accagtttat ccggggttat tgccagtatt ttgctgatca gctgcccgcc
    18841 attccaactg agacgcaaag gaattgcggc tttgtgcaaa tcgcgtaaca cgcccaggac
    18901 ggctaacgga ttttgtttca ggaactgctc atggtaatga ctcacgcgga aaaactcctg
    18961 actgacaaac tgtctttggt ttatcggtca tagttaacaa aacttaatac aaatgcgtga
    19021 atatttttta catgttgttc ttaaatcagc cgacaaacgc ccagcatcga tcccgccctt
    19081 gcctatactt agagcgttga cgtaagcatt tcttgcgacc gattcatcga aacgagggcc
    19141 tgaacatggg aattattgcc tggattattt ttggcctgat agccggcatt atcggcaagc
    19201 taatcatgcc ggggcgtgat ggtggtggat ttttcctgac ctgtattctc gggatagtcg
    19261 gtgcggtggt cggcggctgg ctggcgacca tgtttggcat tgacggctcc atcagcggtt
    19321 ttaatttgca cagcttcctg gtggcggtgg tgggcgctat cctggtgctg ggcgtattcc
    19381 gcctcctgcg aagagaataa gattttcata aggcggatag cgatacagat gccgctatcc
    19441 gctttcacat cagaacgtat actcgacacc tgcccagaaa ttgcgacctt cttcaataaa
    19501 cccttcactg taggcatatt gggtatcaaa caggttattg accgacgcat tgacgctgaa
    19561 gccatgacct aaggtgtaat cggctcgaat gtgggtcacc gcaaaaccgg cggctttttg
    19621 tgaaccgtca ctgttgctgt agctggagga acgcgcctct tccgacagcg ttacgcttaa
    19681 cggctcccat ggtttgagtg tcatccatgc ggtcattgtc tgcgttggaa gatcggttat
    19741 cttgccgatg tctttgcgtt tggcgtcagc gtggatcaga gcgtagctca atcctacatc
    19801 cagtatattg ctgattttcc ccttaatacc ggcatccaga ccgctgtaat ccaccgtgcc
    19861 gctgttctga ttttgaatgg tatcggcatt gatattgtgc gagaggatgg catcactcac
    19921 ccggttatag taaacgctga cctcaaatcc ccagtcgcgc gtgaaggcac cattccaggt
    19981 taaatccacc ccgcgagcgc gttccggctt gagctgcggg ttaacaatcg ctatctggtt
    20041 atacgcaggt ttggatgtgg tatagcgttc tttcagcgtc ggaaagcgtg tgcggtcata
    20101 gtacgaaagc gccagagtgt cttcattggc aaagtggtat ttccccatca cctgccagtt
    20161 aaaagctgac tgattgttgt cgtcataatg ggtgatgctg ccatctttct cgtgtttttt
    20221 cgcttctacg ctatcgcgcc agtcatagct gattcccgcc acgacatcga cattatcagc
    20281 agcagcccat tgatattcac tggcgagcga ccaggtacga tcttcatagc gatcgtaagc
    20341 ggcgtgcggc gcaccttttt cccggtgtac gtcatctttc cagttaacgg caaacgacag
    20401 cagatcgttt tcacgcacat cggctgccag ttgtaatccg gcaccgtcgc tgtaatcgga
    20461 ataatggctg tagctgcctt ttttattttt caaatcagcc agcgagttgt acatcatcag
    20521 cgtattttca aaggtgtcgc gatacagccg acttttcagg gtaaaacggt cgtttagttg
    20581 ggtcgctccc tgataataaa aactttcttt gtcatactct ggccactgcc agtagcgtga
    20641 tttttgacca caatttccgc tgtatggcgg gttatctttt tcaccatcct gcttaatgta
    20701 agtcagcgtg tattcatcgt tttcgcgcgg tgtaaaaccg agcttcacaa tgccgcgttt
    20761 atcatcagcc gatgaattaa tcatcttgcc gtgtttgcct gcaatatcat tatttacacc
    20821 atgcggcagg ccgagaaaat cctgctttag ctggctaccg ctgacttgca aatacccaag
    20881 atcgctgctg gcggcaaatg aagcatgcat atcgtaggca ttgtcctgac tacggctcca
    20941 tccctggcga tatcccagac ttgcttcttc agggtggtga tattaatggc tccgcccatc
    21001 tgattaggcc catgaagcag cgacgaatac cctttggaaa cttcgactgc ccccagattg
    21061 ttggtcagaa tccgcgccag atcgaggttg ccgtcatagg ggacataaat gggcacaccg
    21121 tcgaaataga ccggcacctg ccgactatca aagccacgaa cttttacctg ttcttcgttg
    21181 cggctgcctg acttttgcag caccacgccg gggacgacac ttaatgcctg agcgacattc
    21241 tgtttatcca aggccttcat ggtgggttga tcgagaacgg tggtcgtcgt cgatgatacc
    21301 ggactggacc atacggtcag cgtgtcgctc tcctgagccg ctacggcctg ctgggtaaag
    21361 gcaagtgata atgctgtgca gagataacgt tttttgagtc tcatagaata aattccgttt
    21421 aacaaagaat taaatagagg ctgtgggttg cagactgact ttaatgtcag ctggtgccgc
    21481 gtaataaggc gcggaggtaa taaaaaggcg aatgccgcag tcgaggtaat ttttgagtgt
    21541 cgtcagatta attccgccgg tcagagccag cgtgcagtga ggagccagcg acggcgctat
    21601 ttgcgcaatt tcagttgcct gttgcggact aaatttgtcg agctgaagta cgtctggttg
    21661 tgcgcgtaac gcggcgatag cctctttcgg cgtgtcggct tcaacaacaa ttttcttctc
    21721 gggtgcgtgg cgacgtaact gagcgattgc gcctgaccag ccctgattgt catgaagaaa
    21781 atggcggtgg ttggcaaaca ataatatggt ttccgcacat ccggcacgat gaattagtcc
    21841 tccggcagcc agaattgcct gcgaagccag taagcgggtg cccggaattg cttttcgggt
    21901 gcaggcgata ttgccgtcag gataacgttc acgaagcaat gccagcattt gagcgagata
    21961 atcagaaaca ccgcaactcc actccagcac attctggact gccttccatc cctgatgaag
    22021 tgctgcggca ttaccctgcg cacggattag ccgttgaccc gcgttcgctt gtgaaccgtc
    22081 gctgaccgcg tcatcaatag ttaatcccag cgtagttaac atcttacacg cgacagaaac
    22141 gccgctgacg cagccgccct gacggtgaaa aaacgctata tagccaggct ggtgtccaat
    22201 atttaatgct cgcgtggtca ggtcacctcc ctggatatct tccagcagta acgcatcgat
    22261 ttgtgcctga gaaaggaaaa tcatcacagt gcctcctgcg gtacgctatc ccaccagatg
    22321 agcgcccagt cgcggctggc aggggcgagg gcacgagcat cttgttgttg ataccagtga
    22381 tataaccgct cacgctcatc gtcgttaagc gcgccaagac tccagctgac gttctgctca
    22441 aaacgttccc aggtgctgtt atcctgttgg caattttgtc cacggataaa atcaacgtgg
    22501 gcataaattc ccatctgata caggacgttg agcgcaaaga tatagttggg taattcgatc
    22561 acttcccgac cagccgcccg ctgaatggct ggagagacga acgaggtgct gaccagatga
    22621 gttgtataga cgcgaagccg tgcctgatta ttcagtttgc tcattgcatg gcgcatatca
    22681 gccaccagag tggagcggga agccacggca atatcgcagc gtggcaggtc actccagtcc
    22741 tcttcccagg cgcgctggat ccagtggaca ttgtcagcct tcagtgcggc ggcgcggcgg
    22801 gcggcaacgt tcagcatccc ctgactgtaa tcaacgccat aaaccgtagt gagtttgtca
    22861 gccagcgcca gcgatacggt gcctggtccg cagcccatat cgaataaggt ctgagcgcct
    22921 tgcaggtcaa ttttagcgat cagttgttgc aggtagctgt ccgttggcga ggcgcaattt
    22981 tccgccattt tctccgcccg ttgatcccag tgatcgggcg ttttttctgt gcggtgagcc
    23041 agtttgagtt gctgcagata aaggtctgca aaatcaatat catcaatgag catgattgtt
    23101 ccttagcggc taattgcaga gaggtgatga tgaatttggt cggcagaaac ccgatacagg
    23161 gccgccagtt tgttcgttgt taactgttcg gtcggtaagc cttgtgttac tcgtccatct
    23221 ggctctactt ggataatgct gtcggcaatg gcgttggcat ggagcgtatg gtgagtcgac
    23281 attaacattg tcatgccgtt atttttgagc tgcgccagcg tgtccagaag ttggatctga
    23341 tgaccgaaat cgaggctgga ggcgggctca tccagtaaca acaagcgcgg ttgttggacg
    23401 agtgcacggg cgattaaagc cagctgacgt tcaccgccgc taagcgtatt ccagcgtcgt
    23461 gtcgccagat gcaaaatgcc cagcttttcc agttgctccg tggctttcag gcgttcttgc
    23521 ttaccgggaa cggaaaaagc gcctatggtg ggggccaggc ccatcaacac catatccagc
    23581 acactgaaag caaaaatacc gtcgtgcgcc tgcggcaccc aggcaatagc ctgtgcgcgc
    23641 tggcgatggc tgagtgtctg cacagagagg ctatccagta ggatctcgcc ttttaataac
    23701 ggtataacgc caagaatgga gcgcatgagt gtggttttgc cgcaaccatt cgcgccaagc
    23761 agacaacaaa ttgttccttg cgacagggtg aaggacacgt cgcgtagcac tggatggcgg
    23821 gagtaaccca gagacgcatg acgaacggca agcaaagtca ctggcgacct ccgcgtaaca
    23881 acaaaagcag gaaaaacggt gcgccaacaa aagcggtcag aatgccaagt gggatttctg
    23941 ttgttccaat gctgcgcgcc agcgtatcgg taagcaggag cagaatagcg ccagtacaca
    24001 ttgccatagg caacagctgc tgatggttat gaccggtcaa caagcgggca atgtgcggaa
    24061 cgaccagacc gatccaacca attatgcccg ctattgctac cgtgctggcg gtgattagtg
    24121 tggcgcagac aatcaatcca aagcgcagac gcgtcacgtt cagtcccagc gaacgggctt
    24181 cgtcgtcgga aagcgtgagt aaattcatgc gccagcggag aaaaaacagg ggcaaggagc
    24241 ctatgagaat gatcggtgca gcatagcaga gatcgcgtaa ggtcacggtg gacagtcccc
    24301 ccagcaacca gaaggtaatt gacggtaact gggtgtaagg atcggccagc gttttaatta
    24361 gcgatatccc cgctccgcac aacgttccta acgcaatgcc taccagcacc agggttaaga
    24421 tcggatcgtg gcgggtcacc cgacgcgtaa tcaaccagac gcccgcgacc accatcagcc
    24481 cgccgcaaaa tgcgtacagc tggatgtaaa caatgggtaa tccccacagg attgccgcgc
    24541 aggcaccaag tccggcaccg gcggagacgc ctaaaatatc cggtgaaact aagggattac
    24601 gcaacattcc ctgataggtg gttccggctc ctgccagcgc tgcgcccagt aacagcgcag
    24661 caagaatacg cggcacgcgg atttgccaga agacaatttg ttcctgcaca gactcatttt
    24721 cttgacgaag aattagcgcc agcagttgct gcatatcgag atgataagcc ccacttacgg
    24781 cggcaaaagt gatactgata gctgccagca gcaaaataca tccgtttacg attctcatta
    24841 gctcgccacc aatttttgat agtcggcatc actgagtgaa caatgccaga acagttgggc
    24901 gtaatgctgc atgtcacttt taaactggcg attgatggcg ggatccagcc aggcgtgaag
    24961 tctgcgtaat cccagaagac ggttgattcc cggcggggca tcgagccagc caaagggcaa
    25021 gccacttaaa aataagatgc gttgttccgc aacggctttc acgccctgcc agagaggatc
    25081 acgacgaata aaatctgcag taacggcctc ctgaaccaga ataatatccg gctgccagcg
    25141 caggagattt tccatggaaa cctgtgtcag gccgtggcga tcggctattt gcgcgacgtt
    25201 gtgcaaacct aataattccg ccgcctcggt atgcaacgaa ccctgtaagc ccgtttccag
    25261 ccctcgggga ccgcgcgcag cataaaagcg gaggttagcg gcgggtgagg tggcgaatgc
    25321 ctgagcttca ccaacgaagt ggctggcgag attggcttgt tctgcggcgc ggtgctcttc
    25381 gcctaacgtt ttgccaagcg ttgttaactg ttctgctgat tgcgccagtt ttccgttaag
    25441 caataaccag gggatttgtg tctgttcgct aacctgccgt gcctgggaga tcaaggtttc
    25501 atccgtattg ccgcaatcaa cgaccaaatc gggatgtaac gccataagcc cttccagcga
    25561 tagtgtgctg gcgcgcccgg cgagtcgtcc cagcctgggt aactggcgaa tgtgctctgg
    25621 caatggaatt aatgcctgac gggcaaaatc aaaagaggaa aaaccgacca ttttttctgg
    25681 cgcgacagcc agcaagagca aatcggcggg cgcgcctgcg ctgacgatgc gtgtaatgtt
    25741 ttgggcttcg ggaagagatt gcgaagcaaa acgattaact gtttgtgaaa aggacggtag
    25801 agactgcagg agcagggtag atgcaagtgc ctgcgcaaac gtccgtctgg ttatgggcat
    25861 ggtgaacatc ctgaaaagta aaatcgatat atactttcat atatatcgat aaaaatcatg
    25921 ttcaatatca aagtttagct attgaaaaca tgaagatgtg acgacttccg caagcctgtt
    25981 gaccctctct atcattaaaa atgtatttat caggtggcta atattattta aatcatttaa
    26041 ggctaaatta tttaaatatg aatgacatta ttgttttttt atcgtccacg gttacgcgac
    26101 gttgaagtta cttgttttat aatgagcgat ttttgatatc tcgccgagaa aattatcagc
    26161 attctctcgt ttcagtgtcg tgaatatact gttaacggag gtgaaaagaa tgctgggggt
    26221 tatacaaaac ctatgttgtt gtcgtccgga ttcaccgctt gctgatagta agcagcgtaa
    26281 ccagaagtta ccgcaacaag actttgagga ttttaagaac gacgttatta atgtcattaa
    26341 caacaatgaa gatatttatt ttcttagaga gagtaattta ctgaatgcgt ttattcggac
    26401 gtgcaaaaaa agagatgata acagttacgg aaatatgata tttacgcttt attttgataa
    26461 cgatatctat gggaacagaa ttatcaaagc a
//
LOCUS       DAJTUI010000054        26018 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTUI010000054 DAJTUI010000000
VERSION     DAJTUI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.054"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            247..354
                     /locus_tag="OO464_002393"
     CDS             247..354
                     /locus_tag="OO464_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6227517.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(441..2120)
                     /gene="bcsG"
                     /locus_tag="OO464_002394"
     CDS             complement(441..2120)
                     /gene="bcsG"
                     /locus_tag="OO464_002394"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR6227518.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2117..2308)
                     /gene="bcsF"
                     /locus_tag="OO464_002395"
     CDS             complement(2117..2308)
                     /gene="bcsF"
                     /locus_tag="OO464_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6227519.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2305..3876)
                     /gene="bcsE"
                     /locus_tag="OO464_002396"
     CDS             complement(2305..3876)
                     /gene="bcsE"
                     /locus_tag="OO464_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6227520.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            4149..4337
                     /locus_tag="OO464_002397"
     CDS             4149..4337
                     /locus_tag="OO464_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR6227521.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4349..5101
                     /gene="bcsQ"
                     /locus_tag="OO464_002398"
     CDS             4349..5101
                     /gene="bcsQ"
                     /locus_tag="OO464_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6227522.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            5098..5457
                     /locus_tag="OO464_002399"
     CDS             5098..5457
                     /locus_tag="OO464_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6227523.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5454..7715
                     /gene="bcsA"
                     /locus_tag="OO464_002400"
                     /pseudo
     CDS             5454..7715
                     /gene="bcsA"
                     /locus_tag="OO464_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7726..10065
                     /gene="bcsB"
                     /locus_tag="OO464_002401"
     CDS             7726..10065
                     /gene="bcsB"
                     /locus_tag="OO464_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR6227524.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            10072..11178
                     /gene="bcsZ"
                     /locus_tag="OO464_002402"
     CDS             10072..11178
                     /gene="bcsZ"
                     /locus_tag="OO464_002402"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6227525.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            11255..11596
                     /locus_tag="OO464_002403"
     CDS             11255..11596
                     /locus_tag="OO464_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6227526.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11511..11762
                     /locus_tag="OO464_002404"
                     /pseudo
     CDS             11511..11762
                     /locus_tag="OO464_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            11844..13832
                     /gene="hmsP"
                     /locus_tag="OO464_002405"
     CDS             11844..13832
                     /gene="hmsP"
                     /locus_tag="OO464_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6227527.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            14023..15309
                     /gene="dctA"
                     /locus_tag="OO464_002406"
     CDS             14023..15309
                     /gene="dctA"
                     /locus_tag="OO464_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6227528.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15530..17026
                     /locus_tag="OO464_002407"
     CDS             15530..17026
                     /locus_tag="OO464_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6227529.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(17122..18051)
                     /gene="kdgK"
                     /locus_tag="OO464_002408"
     CDS             complement(17122..18051)
                     /gene="kdgK"
                     /locus_tag="OO464_002408"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6227530.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            18283..19050
                     /gene="pdeH"
                     /locus_tag="OO464_002409"
     CDS             18283..19050
                     /gene="pdeH"
                     /locus_tag="OO464_002409"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6227531.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            19120..21180
                     /locus_tag="OO464_002410"
     CDS             19120..21180
                     /locus_tag="OO464_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6227532.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21362..22684)
                     /locus_tag="OO464_002411"
     CDS             complement(21362..22684)
                     /locus_tag="OO464_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6227533.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(23095..24108)
                     /gene="yhjD"
                     /locus_tag="OO464_002412"
     CDS             complement(23095..24108)
                     /gene="yhjD"
                     /locus_tag="OO464_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6227534.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(24157..25056)
                     /locus_tag="OO464_002413"
     CDS             complement(24157..25056)
                     /locus_tag="OO464_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6227535.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            25576..>26018
                     /locus_tag="OO464_002414"
     CDS             25576..>26018
                     /locus_tag="OO464_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6227536.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQR"
BASE COUNT         6199 a         6538 c         7089 g         6192 t
ORIGIN      
        1 gcagcacaga gtgaagacgt gacggattcc ggaacagaaa aacagtcgta gaatcagcgg
       61 caagccggaa aggttccggt gaggcgcaat gttgcggggg ctttatccct ggtggcattg
      121 gttgctggag agagaaaacc cccgcacgtt gcaggtatgc acctgacaac atcacggggg
      181 ctaatcttga acccaaacaa ctcaagaata gccgcgaaac gttggcatta caacacaggc
      241 ggctatatga cgctcgcaga actgggcatg gccttctggc atgatttagc ggctccggtc
      301 attgctggca ttcttgccag tatgatcgtg aactggctga ataagcggaa gtaacgtgtc
      361 atgcgggcgt caggctgccg taatggcaat ttgcgcccgg atcaggccgc agaggttaaa
      421 ctctgcggcc tttttcgtgc ttactgcggg taaggcaccc agtcgccgcc gttcaggcga
      481 acgtacggtt tatcttggta ttgaataact actgcatttg agttctcgga gaccggtgct
      541 gtttgtggca acccactggt gagttttttc cagtcaacat tgtcttcggt gaaaatcttg
      601 ccatcaagaa cgcgaaccac cagatcggag atagccagga agctgctcgg ttggtcaatg
      661 acaatcggtg ccccctgatg tggtgccttc atgccgaaga atttcacccc aacggggacg
      721 tctgtgatag acgggctagg gatatcacgt aggccagata cctgcattct gtcgcccttc
      781 agcgcgccgc cgtgttccgg caccacgacc accatcactt tacgacccga tttctccagt
      841 tcagtaaaga aggcgtccag ttcatcaaag aatttctgcg cccgcgcttt gtaatccgct
      901 gttttgctga cccccggata atggttgccg tcatgcagtg gaagcgtgtt gtagaacgtg
      961 gcactacggc tgtttttatc tttttcggta acgtccagcc agcggttaag cacggcggta
     1021 tcgtcataaa ccggcgaacc atcaaagccc agcaaaataa ccggcagatt tgtttgatcc
     1081 atcaattcag tctgcatgcc gccattttcg cgaacttctt tcaaaaaacc gccgaactgg
     1141 ccgttatgtc ccatcatcag gtgctgggta aagcccagtt tcgaaaggtt atcaaacaga
     1201 tagcagtcgt tatttgccgg ttgatacaga ttagtgtgcg aagtctgccc gcagctggcg
     1261 cgcagtaaac ggatcgccgc cgggccactg taggaggtgg cggagttaaa gttcttgaaa
     1321 tcaatatcga aatgcgacca cagtggatgc gacatcaacc cggcggcttc tatatccgac
     1381 caggaaagcg aacagatgtt aatcaccagt agttcaaatg gctgagcatc agcgggcagc
     1441 gaagacggga aggtcgattt acgtttcgcc tccgcgttat agaaattatt cagccaggcg
     1501 ttaaggttcg ccgttgttgg cggtgcagtt tgtgcgggca tatcacccac taccggtgcg
     1561 ccacccgtcg ccgcaacggt tgccgctgcg ttaccacccg tcgttgttac agtggtcgtc
     1621 ggttgtccgg ctggccacaa ggagaaactt ggtcccgcca gggtaagtac gttcagccat
     1681 agcagtatgg caaccacaaa aacggtaatg cgaatccatt gtgacaggaa taaccaggcc
     1741 actaataaaa caaaaatggc cccaatcatc tgccagttaa taaagcgtgt gacaaggtcg
     1801 attaaataat cggtactgaa ccccgccacc tgcgaaccct ggctcattat gctttccggg
     1861 ccaggcaacc agatgtcatg ccagaacaaa gcaaagccga tcggcagggc aatccagtgg
     1921 cgcaagcgat gcaggctgta acgcggaatg ggcatcagca gaaacgcggc aaacaccaaa
     1981 ttgaggagcg ggtggaagtt aagatatccc gcccacaaca ggccaaactt aaccagaaaa
     2041 tagaagttcc agccggaaag gccgcgccag tattgccaga gggaagaagg catggcggta
     2101 ttttgcgtaa attgagtcat tttttggttg ccctggcttt ttccgtgcgg cgtaacgtcc
     2161 cggccggttt aacataacga ggtttagcaa agaacaaacg taaggtgtcg cgaatgcgtc
     2221 gcaaagagtg ccgcgccaga tagcccagcg ggaaaaatat cagtgcgcaa acgataataa
     2281 tttcaatgat atcgctgatg gtcatcatga tgagcgctcc acagcatcat ctaacagtcg
     2341 catgggttct ggtattcgtc gccagtggcg accatcgtgc tcggcattga tgaccggttt
     2401 agaactttgc gttaaaggca gcggcattcc ccattgttct ggggcaagca ggcgcatctg
     2461 caccagctcg gcactgattt gatcatcttc aaaccagacc atacggtttg agaaaatgtc
     2521 gccagtaggc aatgggaaaa tatgattcaa cgcggtatcc agatcgttaa tccgacagaa
     2581 tgagagaaac agcaccagcc gattaccgcc aatggtcatg atatcgccgg tacggttagg
     2641 gcgacatagc gtcagggctt gctcaacgcg gatacccggt accggacgta gggcaaccag
     2701 aacgccttta ccgtgggcag gcaagagagg gttattcatc atgttgttga cggcattaca
     2761 gaacacatcc cacttctgga aaccacgcag tttgagcggc tgggtcattg acagcaaggt
     2821 agtgatatct tccggtacat agcgactaaa cttctgccct tgcacgcttt cgatcatcgt
     2881 cagacaacgg gagagtggcg cattccacgg aataaccata tttgcaccgc aggccaataa
     2941 caaacgttca tcggtggcgc gcaggctagc ggtattttcc cgcacgagga ttttcatcgc
     3001 actaccgcgc tggcgacgca gggtatgaat gctgcgggcc agtggctcga tttgcgcatt
     3061 ttgctgtaaa gaaaagacca ccgtcgccgc ctgagcggta cgggcttcat tgaacaggac
     3121 ttcgttattg ttgaacagtt gccagtgttc cgatagcggc ggcgcacctt ccagtacagc
     3181 aacattactc agaatgcgtt tttcgtcgct gcgtggttgg atctccgcct cttcgctttg
     3241 aactaatgtc cagataccat tttgttgctg aacgctaagc tgctgacggg cgctgacccc
     3301 tttttcgttg caccagaagg caatatccag caaatgttgg tcaccctgaa aacgcaaact
     3361 ggcaagacca aaaagtgaac ggtattcctc aagcaacaat gaaaattgtt tatcgttatt
     3421 atttccggga ttaattacca aaagcgaaca atggtttaac ctgctccatt tattcatttt
     3481 atccaaccat gagcgaagcc gctcggcagg aatattttgc catgcgttat ttgcgcaaac
     3541 aagaataaaa aggtaattat ggggatcaat agaacactgc aaatcacggg ccaaatagta
     3601 tagacctttt tcatgattta gcattgaaaa taattttatt ttttccggcc cttgagaatc
     3661 atctaattga aagattttcg ccggatcgct gtccatgcta atgaccgcga cgtgtgcggt
     3721 ttcagcctgg gatgcaattg tttgattcgc cagactgata gcatcttcat ggcgatcgac
     3781 gttaaaccac cagacgccgc ctgctggcat atgtcgcagc tcatcccata atgatgagat
     3841 accgatagag aatacagggt ccacaatgtc cctcatgtca ccattttgtc tgattatcag
     3901 tttactagcg aaagctaaga aataaaccta ccattgaaat taaaacttat cagatttagc
     3961 atgtgaatca atttagaagg tcacaagacg tgtgatttta gtcttttcgt ttttaacttt
     4021 ttctgttgcg taacggaaag tcaaaaagtg agcaaattcc cgtctcgccg taaacaataa
     4081 accggcgaaa tgctcacggt tagaattgtc tcatgaatgg tacggttatt tcatagggat
     4141 caagcaaaat gaataacaat gaaccagata ctctgcctga tcccgcgata ggctatatct
     4201 tccagaatga tattgtggcg ttaaagcagg cattttcact gcctgatatt gattatgccg
     4261 atatttccca acgcgaacag ttggccgcgg cattaaaacg ctggccgttg ctggcagagt
     4321 ttgcgcaaca aaagtagggg attggtgaat ggccgtactg ggattgcagg gggtgcgggg
     4381 aggcgtgggg acaacaacca tcaccgccgc attagcctgg tcattacaaa tgttgggaga
     4441 aaatgtcctg gtggtcgatg cctgcccgga caacttgttg cgcctgtcat ttaatgttga
     4501 ttttacccac cgtcagggct gggccagagc gatgctggat ggccaggact ggcgtgacgc
     4561 tgggttgcgc tacacctcgc agctcgattt gctgcctttt ggtcagttat ccattgaaga
     4621 acaagaaaat ccacagcact ggcaaacccg gctgagcgat atttgctccg gcttacagca
     4681 actaaaagcc agcgggcgtt accagtggat tttaatcgac ttaccgcgtg atgcctcgca
     4741 gataacccac cagctgctga gtttgtgcga gcactcgctg gcaatcgtca atgtggatgc
     4801 caactgccat atccgactgc atcagcaagc gctgccggat ggcgcacata ttttgattaa
     4861 tgacttccgt attggcagtc aggttcagga cgatatttac cagctttggt tgcaaagcca
     4921 gcgccgatta ctgccgatgc tcattcatcg tgatgaagcg atggctgaat gcctggcggc
     4981 taagcaacca gtaggtgaat atcgcagtga tgcgctggcg gctgaagaga tactgacgct
     5041 ggcgaactgg tgcctgttga actactccgg gctgaaaacg ccagtcggga gtgcatcatg
     5101 agtatcctga cccggtggtt gcttatcccg ccggtcaacg cgcggcttat cgggcgttat
     5161 cgcgattatc gtcgtcacgg tgcgtcggct ttcagcgcga cgctcggctg tttctggatg
     5221 atcctggcct ggatttttat tccgctggag cacccgcgct ggcagcgtat tcgcgcagaa
     5281 cataaaaacc tgtatacgca tatcaacgcc tcgcgtccgc gtccgctgga cccggtccgt
     5341 tatctcattc aaacatgctg gttattgatc ggtgcatcgc gcaaagaaac gccgaaaccg
     5401 cgcagcgggc attttcaggt ctgcaaaata ttcgtggacg ttaccatcaa tggatgaacg
     5461 agctgcctga gcgcgttagc cataaaacac agcatctgga tgaaaaaaaa gagctcggtc
     5521 atttgagtgc cggggcgcgg cggttgatcc tcggtatcat cgtcaccttc tcgctgattc
     5581 tggcgttaat ctgcgttact cagccgttta acccgctggc gcagtttatc ttcctgatgc
     5641 tgctgtgggg ggtagcgctg atcgtacggc ggatgccggg gcgcttctcg gcgctaatgt
     5701 tgattgtgct gtcgctgacc gtttcttgcc gttatatctg gtggcgttac acctctacgc
     5761 tgaactggga cgatccggtc agcctggtgt gcgggcttat tctgctcttc gctgaaacgt
     5821 acgcgtggat tgtgctggtg ctcggctact tccaggtagt atggccgctg aatcgtcagc
     5881 cggtgccatt gccgaaggat atgtcgctgt ggccgtcagt ggatatcttt gtcccgactt
     5941 acaacgaaga tctcaatgtg gtgaaaaata ccatttacgc ctcgctgggt atcgactggc
     6001 cgaaagacaa actgaacatc tggatcctcg atgacggcgg cagggaagag tttcgccagt
     6061 ttgcgcaaaa cgtgggggtg aaatatatcg cccgcaccac tcatgaacat gcgaaagcgg
     6121 gcaacatcaa caatgcgctg aaatatgcca aaggcgagtt cgtgtcgatt ttcgactgcg
     6181 accacgtacc aacgcgatcg ttcctgcaaa tgaccatggg ctggttcctg aaagagaaac
     6241 agctggcgat gatgcagact ccacaccact tcttctcgcc ggacccgttt gaacgcaacc
     6301 tgggacgttt tcgtaaaacg ccgaacgaag gcacgctgtt ctatggtctg gtgcaggatg
     6361 gcaacgatat gtgggacgcc actttcttct gcggttcctg tgcggtgatt cgtcgtaagc
     6421 cgctggatga aattggcggc attgctgtcg aaaccgtgac tgaagatgcg catacttctc
     6481 tgcgtttgca ccgtcgtggc tatacctccg cgtatatgcg tattccgcag gcggcggggc
     6541 tggcgaccga aagtctgtcg gcgcatatcg gtcagcgtat tcgctgggcg cgcgggatgg
     6601 tataaatctt ccgtctcgat aacccgctca ccggtaaagg actgaagttt gctcagcggc
     6661 tgtgctacgt caacgccatg ttccacttct tgtcgggcat tccacggctg atcttcctga
     6721 ctgcgccgct ggcgttcctg ctgcttcatg cctacatcat ctatgcgcca gcgttgatga
     6781 tcgccctatt cgtgctgccg catatgatcc atgccagcct gaccaactcg aagatccagg
     6841 gcaaatatcg ccactctttc tggagtgaaa tctacgaaac ggtgctggcg tggtatatcg
     6901 caccaccgac gctggtggcg ctgattaacc cgcacaaagg caaatttaac gtcaccgcca
     6961 aaggtggact ggtggaagaa gagtacgtcg actgggtgat ctcgcggccc tacatcttcc
     7021 ttgttctact caacctggtg ggcgttgcgg taggcatctg gcgctacttc tatggcccgc
     7081 caaccgagat gctcaccgtg gtcgtcagta tggtgtgggt gttctacaac ctgattgttc
     7141 ttggcggcgc agttgcggta tcggtagaaa gcaaacaggt acgccgatcg aaccgcgtgg
     7201 agatgacgat gcccgcggca attgcccgcg aagatggtca cctcttctcg tgtaccgttc
     7261 aggatttctc cgacggtggt ttggggatca agatcaacgg tcaggcgcag attctggaag
     7321 ggcagaaagt gaatctgttg cttaaacgcg gtcagcagga atacgtcttc ccgacccagg
     7381 tggcgcgcgt gatgggtaat gaagttgggc tgaaattaat gccgctcacc acccagcaac
     7441 atatcgattt tgtgcagtgt acgtttgccc gtgcggatac atgggcgctc tggcaggaca
     7501 gctacccgga agataagccg ctggaaagtc tgctggatat tctgaagctc ggcttccgtg
     7561 gctaccgcca tctggcggag tttgcgcctt cttcggtgaa gggcatattc cgtgtgctga
     7621 cttctctggt ttcctgggtt gtatcgttta ttccgcgccg cccggagcgg agcgaaacgg
     7681 cacaaccatc ggatcaggct ttggctcaac aatgatgata acgcgatgaa aagaaaacta
     7741 ttctggattt gtgcagtggc tatggggatg agtgcgttcc cctctttcat gacgcaggcg
     7801 acgccagcaa cgcaaccact gatcaatgct gagccagctg tagccgccca gacggaacaa
     7861 aatccgcagg tggggcaagt gatgtcaggc gagcagggag ctgatgcccc catcgtggcg
     7921 cagaacggtc cttcgcgtga tgtgaagctg acctttgcgc aaatcgcgcc gccgccgggc
     7981 agcatggtgc tacgtggtat taacccgaac ggcagcattg agtttggtat gcgcagcgat
     8041 gaagtggtga cgaaggcgat gctcaacttc gaatacacgc catcgccatc gttactgcct
     8101 gtccagtcgc agttaaaggt ttatctcaat gatgagctga tgggcgtgct gccagtgacc
     8161 aaagaacagt tgggtaaaaa aacgctggcg caaatgccca ttaatccact gtttattacc
     8221 gacttcaacc gtgtgcggct ggagtttgtc ggccattatc aggacgtgtg cgaaaacccg
     8281 gccagcacca cgctttggct ggatgttggg cggagcagtg gactggatct gacctatcag
     8341 accctgaatg tgaagaatga cctgtcacac ttcccggtgc cattctttga cccgcgcgat
     8401 aaccgtacta acaccttgcc gatggtcttt gcgggtgcgc cggatgttgg gctgcaacaa
     8461 gcatctgcca ttgtcgcctc gtggtttggt tcgcgttctg gctggcgtgg gcagaacttc
     8521 ccggtgctct ataaccaatt gccggatcgc aatgccattg tctttgcaac caacgacaaa
     8581 cggccggact tcctgcgcga tcatccggcg gtaaaagccc cggtgattga gatgattaac
     8641 catccgcaga atccttacgt caaactgctg gtggtgtttg gtcgtgacga caaagacctg
     8701 ttgcaggcag cgaaaggtat cgctcagggt aacattctgt tccgtggtga aagcgtggta
     8761 gtgaatgaag tgaaaccgct gctaccgcgt aagccgtacg atgcgccgaa ctgggtacgt
     8821 accgatcgtc cggtcacatt tggcgaactg aaaacctatg aagaacagtt acaatccagc
     8881 ggccttgagc cagcagcgat taacgtttcg ctaaacctgc cgccggatct ctacctgatg
     8941 cgcagtaccg gcattgatat ggatattaat taccgctaca ccatgccgcc ggtgaaagac
     9001 agttcgcgga tggatatcag cctgaataac cagttcctgc aatccttcaa cctgagcagc
     9061 aaacaggagg cgaaccgtct gctgctgcgt attccggtat tacaaggttt gctggatggc
     9121 aaaacagatg tctctattcc ggcgctgaaa ctgggcgcga ccaaccagct gcgcttcgac
     9181 tttgagtata tgaacccgat gccaggcggt tcggtagata actgtattac cttccagccg
     9241 gtgcagaatc atgtggtgat tggtgacgat tccaccatcg acttctcgaa gtattaccac
     9301 ttcatcccga tgccggatct acgcgccttt gctaacgcgg gcttcccatt cagccggatg
     9361 gcggatctgt cgcaaaccat caccgtgatg ccgaaagcgc ctaacgaagc acagatggaa
     9421 acgttgctga atactgttgg ttttatcggc gcacagacgg gcttcccggc gattaatctg
     9481 acggtgaccg atgatggcag caccattcag ggcaaagatg ccgacatcat gatcatcggt
     9541 ggtatcccgg acaaactgaa agacgataag cagatcgacc tattggtgca ggcgaccgaa
     9601 agctgggtga aaacaccgat gcgccagacc ccgttccccg gcattgtgcc ggacgagagc
     9661 gatcgcgcgg cagaaaccca gtcaacgctg acctcttccg gtgcgatggc ggcggtgatt
     9721 ggcttccagt cgccgtataa cgaccagcgc agcgtgattg cgctgttggc agatagccca
     9781 cgcggttatg aaatgcttaa cgatgcggtg aacgatagcg gcaaacgcgc caccatgttc
     9841 ggttcggtcg cggtgatccg cgagtccggt atcaacagcc tacgtgttgg cgacgtttat
     9901 tacgtaggtc atctgccgtg gttcgagcgc ttgtggtatg cgctggcaaa ccatccgatt
     9961 ctgctggcgg tgctggcgac tatcagtgtg atattgctgg catgggtact gtggcgtctg
    10021 ctgcgaatta ttagtcgtcg tcgtcttaac ccggataacg agtaattgaa gatgaatgtg
    10081 ttgcgtagtg gaatcgtgac gatgctgttg ttggctgcct ttagtgttca ggcagcctgt
    10141 acctggcctg cctgggagca gtttaaaaag gattacatca gtcaggaagg gcgcgtcatc
    10201 gaccccagcg acgcgcgcaa aatcaccacc tccgaagggc aaagttacgg tatgttcttt
    10261 gccctggcgg ctaacgaccg tgcagctttc gataatattc tcgactggac gcagaacaat
    10321 ctcgctcagg gttctttaaa agaacgtttg cccgcctggc tgtggggcaa gaaagagaac
    10381 agtaagtggg aagtgctgga cagcaattcg gcctccgatg gtgatgtctg gatggcctgg
    10441 tcgttgctgg aggcggggcg tttgtggaaa gagcagcgtt ataccgacat cggcagcgcg
    10501 ttgctaaaac gtatcgcgcg ggaggaagtg gtgacggtgc ctgggctggg ttccatgttg
    10561 ttaccgggca aagtgggttt tgctgaggat aacagctggc gttttaaccc cagctacatg
    10621 ccgccgacgc tggcgcagta tttcacccgc tttggcgcgc cgtggaccac gctgcgcgaa
    10681 accaatcaac atttattgct ggaaaccgcc ccgaaaggct tttcgccaga ctgggtgcgc
    10741 tatgagaaag acaaaggctg gcagctaaaa gccgaaaaaa cattgatcag cagctacgac
    10801 gctatccgcg tttacatgtg ggtaggcatg atgcctgaca gcgatccgca aaaagcgcgg
    10861 atgctcaacc ggtttaaacc gatggcgaca ttcactgaga aaaacggtta tccgccggaa
    10921 aaagtggatg tggctacggg gaaagcgcag ggtaaaggac cggtcggttt ttctgccgcc
    10981 atgctgcctt ttttacaaaa ccgcgatgcg caggcagttc agcgccagcg cgtggccgat
    11041 aactttcccg gcagcgatac ctattacaac tatgtgctga ccctgtttgg acaaggctgg
    11101 gatcaacacc gtttccgctt ctcgacaaaa ggtgagttat tacctgactg gggccaggaa
    11161 tgcgcaaatt cacactaaac atattcacgc tttccctcgg tctggccgtc atgccgatgg
    11221 tcgaggcagc accaaccatc agcagaccct gcggatggac agcggcaaca ctaatgccgt
    11281 gcgcgggctg gcgaatattt accgccagca gtcgccagaa aaagctgaag cgtttatcgc
    11341 ctcgctctct gccagccagc ggcgcagcat tgatgatatc gaacgcagcc tgcaaaacga
    11401 ccgtctggca cagcaggcag aggtactgga aaaccagggc aaatgggcgc aggcggcagc
    11461 acttcagcgg caacgactgg cgctgggccc cggcagcgta tggattactt accgactggc
    11521 aggaagaagc tgcgcggcaa tccaacgatg gcggcagcag tcagggcttc ggctacacgg
    11581 cgcgggcatt acttgaacga cgtgttactt ccaactggtt tgttggcacg gcaattgata
    11641 tccagcaggt gaaagattac gcacccagcc atttcctgct ctacgtacgt tattccgccg
    11701 ccggatggca gggtgacatg gatttaccgc cgcagccgct gataccttac gccgactggt
    11761 aagttttcag atagcgcctc tcttaatgcc gctgcgatcg ggtatactcg ggcggcaatc
    11821 tgggatttcc ggggggagac aatttgcgcg taagtcgctc gttaacaatc aagcagatgg
    11881 caatggtggc agccgttgtc ctggtgttcg tttttatttt ttgcaccgtt ttgctgttcc
    11941 atctggtcca gcagaatcgc tataacacgg ctacgcaact ggaaagcatt gctcgctctg
    12001 tccgcgaacc cttatcttca gctattttga aaggcgatat tcccgaagcg gaagctattc
    12061 ttgccagcat taaaccggca ggcgtggtca gccgtgccga tgtagtgctg cctaaccagt
    12121 tccaggcgct gcgtaaaagt tttattccag agcgtccggt gccggtaatg gttactcgcc
    12181 tgtttgagct accggttcaa atctcgctgg gcgtctactc gctcgaacgt ccggcaaatc
    12241 cgcagccaat agcctatctg gtgctacagg cggattcctt ccgtatgtat aagttcgtga
    12301 tgagcactct ctcaacgtta gtgaccattt acttactttt gtcgcttatc ctgaccgtcg
    12361 ccatcagctg gtgcattaac cgcctgattt tgcatccgtt acgcaatatt gctcgcgaac
    12421 ttaacgccat cccagcccag gagcttgttg gtcaccaact ggcattaccg cgtctgcatc
    12481 aggacgatga aatcggtatg ttggtgcgca gttacaacct caaccagcaa ttgctgcagc
    12541 gccattatga agaacagaac gaaaatgcga tgcgcttccc ggtgtcggat ttgccgaaca
    12601 aagccttgct gatggagatg ctggagcagg ttgtcgcgcg taaacaaacc accgcgctga
    12661 tgatcatcac ctgtgaaacc ctgcgtgata ctgcgggcgt gctgaaagag gcgcaacgag
    12721 aaattctgct gctgacgctg gtggaaaaac tcaaatcggt actgtcgcca cgtatgatcc
    12781 tcgcgcagat tagcggttat gactttgctg tcattgccaa cggtgtacag gaaccgtggc
    12841 acgcaatcac tttgggtcag caagtactca ctatcatgag cgagcgcctg ccgattgaac
    12901 gtattcaact ccgtccgcac tgtagcattg gcgtggcgat gttctacggc gatctcaccg
    12961 ccgaacagct ttacagtcgc gctatttctg cggcatttac cgctcgccat aaaggcaaga
    13021 atcagattca gttctttgat ccgcagcaga tggaagcagc ccagaagcgg ttgacggaag
    13081 agagcgatat ccttaatgca ctggaaaatc atcagtttgc aatttggtta cagccacagg
    13141 tcgagatgac cagcggtaaa ctggtcagtg cggaagtgtt actgcgtatc cagcaaccgg
    13201 atggcagttg ggacctgccg gatggcttaa tcgatcgcat tgagtgctgt gggctgatgg
    13261 ttaccgtcgg tcactgggtg ctggaagagt cctgtcgatt gcttgcagcc tggcaagagc
    13321 gcggcattat gctgcccttg tcggtaaacc tctctgcgct gcaactgatg cacccgaata
    13381 tggtggcgga tatgctggaa ctgttaaccc gctatcgcat tcagccggga acactgattc
    13441 tggaagtgac agaaagccga cgtattgacg accctcatgc tgcggtggca atcctccgtc
    13501 cgctgcgcaa tgccggagtt cgggtggcgc tggatgattt cggcatgggc tacgcagggc
    13561 tgcgtcagct gcagcatatg aaatcgttgc caatcgacgt actgaaaatc gacaaaatgt
    13621 ttgttgaagg cttgccggaa gatagcagca tgattgctgc aattatcatg ctggcgcaga
    13681 gcctgaactt acaaatgatt gccgaaggcg tggagactga agcacaacga gactggctgg
    13741 caaaagcggg cgttggtatt gcccagggct tcctttttgc tcgcccactc cctattgaaa
    13801 tcttcgaaga gagttacctg gaagaaaagt agctacccca aactgattac aaaactttaa
    13861 aaagtgctgg tttgtgcgag ccagctcaaa ctttttaacc tttttgtttc aattatgatc
    13921 caggtacatt tctgtgatgt tgtctgggtg ttattttaag gccgcaggta cccccccccc
    13981 ccataacctt acaagacctg tggttttact aaaggacacc ctatgaaaac ctctctgttt
    14041 aaaagccttt actttcaggt cctgacagcg atagccattg gtattctcct tggccatttc
    14101 tatcctgaaa taggcgagca aatgaaaccg cttggcgacg gcttcgttaa gctcattaag
    14161 atgatcatcg ctcctgtcat cttttgtacc gtcgtaacgg gcattgcggg catggaaagc
    14221 atgaaggcgg tcggtcgtac cggcgcagtc gcactgcttt actttgaaat tgtcagtacc
    14281 atcgcgctga ttattggtct tatcatcgtt aacgtcgtgc agcctggtgc cggaatgaac
    14341 gtcgatccgg caacgcttga tgcgaaagcg gtagcggttt acgccgatca ggcgaaagac
    14401 cagggcattg tcgccttcat tatggatgtc atcccggcga gcgtcattgg cgcatttgcc
    14461 agcggtaaca ttctgcaggt gctgctgttt gccgtactgt ttggttttgc gctccaccgt
    14521 ctgggcagca aaggccaact gatttttaac gtgatcgaaa gtttctcgca ggtcatcttc
    14581 ggcatcatca atatgatcat gcgtctggca cctattggtg cgctcggggc aatggcgttt
    14641 accatcggta aatacggcgt cggcacactg gtgcaactgg ggcagctgat tatctgtttc
    14701 tacattacct gtatcctgtt tgtggtgctg gtattgggtt caatcgctaa agcgactggt
    14761 ttcagtatct tcaaatttat ccgctacatc cgtgaagaac tgctgattgt actggggact
    14821 tcatcttccg agtcggcgct gccgcgtatg ctcgacaaga tggagaaact cggctgccgt
    14881 aaatcggtgg tggggctggt catcccgaca ggctactcgt ttaaccttga tggcacatcg
    14941 atatacctga caatggcggc ggtgtttatc gcccaggcca ctaacagtca gatggatatc
    15001 gtccaccaaa tcacgctgtt aatcgtgttg ctgctttctt ctaaaggggc ggcaggggta
    15061 acgggtagtg gctttatcgt gctggcggcg acgctctctg cggtgggcca tttgccggta
    15121 gcgggtctgg cgctgatcct cggtatcgac cgctttatgt cagaagctcg tgcgctaact
    15181 aacctggtcg gtaacggcgt agcgaccatt gtcgttgcta agtgggtgaa agaactggac
    15241 cacaaaaaac tggacgatgt gctgaataat cgtgcgccgg atggcaaaac gcacgaatta
    15301 tcctcttaat ctcaccactt atgcccgcac tcccttcccc gagtgcgggt gtttgcgcat
    15361 aattgacccc tgtgcctgaa cttaccacct gggtgagttc aggtttattt cacttcttcc
    15421 gtgacatttt catgttcttg cggtctaaca cgaagtgttt ttaacgtcat ttagactgac
    15481 cgacaacggg cagttttttt attaccagga tagttgatca ggggttcaca tgcagggcat
    15541 aaaaattcga cttttagcgg gcggtttgct gatgatggcc actgctggct atgtgcaggc
    15601 agatgcgctc cagcctgatc cagcatggca acaggggacg ctttccaacg gtttacagtg
    15661 gcaagtgctg accacccccc agcgtcccag cgatcgtgtt gaaattcgcc tgctggttaa
    15721 taccggttcg ctcgccgaaa gtacacaaca gagcggttac agtcacgcca tccctcgtat
    15781 tgcgctaacg caaagcggtg gccttgacgc agcacaggcg cgttcattgt ggcagcaggg
    15841 gatcgaccct aaacgcccga tgccgccggt aattgtctct tatgacacca cgctgtttaa
    15901 tctgagtttg cccaataacc gtaacgattt gctgaaagaa gcgctctctt atctggcaaa
    15961 tgccactggc aaattgacca tcacaccaga aaccatcaac cacgcgctgc aaagtcagga
    16021 catggtggca acctggcctg ccgatactaa agagggctgg tggcgctatc gtctgaaagg
    16081 gtcaaccttg ttaggtcacg atcctgccga tccgctgaaa caacccgttg aagcggaaaa
    16141 aattaaagat ttctatcaga aatggtacac cccggatgca atgacgctac tggtggtggg
    16201 aaacgtggat gcgcgctcgg ttgtcgacca aatcaacaaa acgtttggcg aactgaaagg
    16261 caaacgtgaa acgccagctc cggtgccgac gctttctccg ctgcgtgcgg aagcggtgag
    16321 tattatgact gacgcggtgc gtcaggaccg gttatctatc atgtgggata cgccgtggca
    16381 gccgattcgt gaatcagccg cactgctgcg ctactggcgt gcggacctgg cccgcgaggc
    16441 gctgttctgg catgttcagc aagcgttaag cgccagtgac agcaaagaca tcggtcttgg
    16501 atttgactgc cgtgtgctgt atctgcgtgc gcagtgtgcc atcaacatcg aatcaccaaa
    16561 cgacaagctg aacagcaacc ttaatctggt ggcgcgtgaa ctggcgaagg ttcgcgataa
    16621 aggtctgccg gaagaagagt tcaatgcgtt agtggcgcaa aagaaactgg agctgcagaa
    16681 actgtttgcc gcctatgcac gggctgatac cgatattctg atgggccagc ggatgcgttc
    16741 gttgcaaaat caggtcgtcg atatcgcgcc ggagcagtat cagaaactgc ggcaggattt
    16801 ccttaacagc ctgactgtgg agatgttaaa tcaggatctg cgtcagcagt tgtcgaatga
    16861 tatggcgtta atactgctgc agccgaaagg cgagccggaa tttaacatga aagcgttgca
    16921 ggcggcctgg gatcaaatca tggccccatc taccgccgct gcgaccacct ctgtcgccac
    16981 ggatgacgta catcctgaag tgacggatat tccacctgca cagtaatgcc tgatggtgat
    17041 gagttagcac aaccatcagg tatttatgga tgagctggat agaggggtaa cactttatcc
    17101 agccttttgc atatgctgcg tttacgctgg catcgcctca cgcgggataa tcgcgccgcg
    17161 atactgaata acggtacttg cggtcaggtg cccgcgtttc gccgcgtttt ccgcactgcc
    17221 gcccgtcaaa cgtaccgcca gataaccggc gctgaaagag tcgccagctg cggtggtatc
    17281 gatcactttt tctttcggca gtttcaccgc cggaacatca actaacgctt caccagcaat
    17341 ggacaccagg caagaatccg ccccgcgttt caccaccact tctttcacgc ccgcgttatg
    17401 ggtgcgcgca atgacgtctt ccaccggctg ttgaccccac agcgcgtctt cgtcgtccag
    17461 cgtcaggaag gcgatatccg tgcattccag catttgctgg tacacctgct gtgtctcttc
    17521 tttgctggcc cacaggcgcg gacgatagtt attgtcgaaa atcacttttc cgcctttggc
    17581 gcggcattcg cgcagcaggg aaagcagctt ttcgcggctg gtcgggctta agatcgccag
    17641 gctaatcccg ctcaggtaga gataatcgaa attcgccagc tcttcgcaaa tcgccgcaga
    17701 ctgctcgctc gccagccaga atttggcggc ggcttcgtta cgccagtagt agaacgtacg
    17761 ttcgccggtg ctgtcggttt caatgtagta aaggcccgga agacggtttt ccatccgttg
    17821 ggtcagggaa gtatcaacgt tctcgccgtg ccaggcgtcc agcatctgct ggctaaaact
    17881 gtccgttccc agcgccgtta cgtaatgaac ggttaatgcc gcaggatcga cctgacgggc
    17941 gatatagacg gaagtgttca gggtatcgcc gccgaaaccg cgcttaacgt ccgcgccttt
    18001 ctcggaaagc tcaatcatgc attcgccaat cacggcaatc tttttggaca tagtcgtgaa
    18061 cctgatctgt aaaaaattag cgttagtttg cgctgtggtg gtttgctggt caaccatatt
    18121 aaaacagtgt tccattattt ttttgagcca gaacgtattc ctgaaagatt ccgttgtgga
    18181 gtgaggaaat ttaccccggc caggcggtaa agttctgccc ttacgcgccg ataatctttg
    18241 tcgagtccgg gcagcatcac ttttaaacac aggacatctt tgatgataag gcaggttatc
    18301 cagcgaataa gcaaccctga agcaagcatc gagagcttgc aggaacggcg tttttggttg
    18361 cagtgtgagc gtgcttacac ctggcagccg atctatcaaa catgcgggcg gttaatggcc
    18421 gtggagctat taacggtggt cacgcatccc ttgaaccctt cgcaacgcct gccgccggat
    18481 cgctatttta ctgaaatcac cgtcagccat cggatggagg ttgtgaaaga gcagattgat
    18541 ttgctggcgc aaaaagccga cttctttgta gagcacggcc tgctggcatc ggtcaatatt
    18601 gatggcccta cgctcatcgc cctgcgtcag caaccaaaaa tcctgcgcca gattgagcgt
    18661 cttccctggc tgcgtttcga actggtggag catatccgtc tgccgaaaga ttcaaccttt
    18721 gcctcgatgt gtgaatttgg cccgctgtgg ctggatgatt ttggtaccgg gatggcaaat
    18781 ttctctgcac taagtgaagt gcgttatgac tacatcaaaa tcgcgcgaga actgtttgtg
    18841 atgctgcgtc agtcgccgga aggccgcaca ctcttttctc agcttttaca tctaatgaat
    18901 cgctattgtc gcggggtgat tgtcgagggc gtagaaacac cggaagagtg gcgtgatgtt
    18961 cagaactcgc ccgcattcgc cgcacaaggc tggtttcttt cacgcccggc accgatggaa
    19021 acgctgaata cggcggttct ggcgctataa gctgcctcat tttccgccgg gctggactat
    19081 ctttaggact ggcacaggaa gaatatgagg aagcgaacga tgagcaaggc aggcaaaata
    19141 accgctgcga tttcaggggc tttcttgttg ttgattgtcg tggcgatcat tttgattgca
    19201 acttttgact ggaatcgact caaaccgacc atcaaccaga aagtctctgc ggagttgaat
    19261 cgtccgttcg ctatccgtgg caatctgggc gtggtgtggg agcggcaaaa acaagaaact
    19321 ggctggcgca gctgggtgcc gtggcctcat gtacatgcag aagacatcat tcttggtaat
    19381 ccaccggata ttcccgaagt cacgatggtg catttgccgc gagtagaggc aacgctggca
    19441 ccgctggcgc tgctgaccaa aacggtctgg ctgccgtgga tcaagctcga aaagcccgac
    19501 gcgcgcctga ttcgcctctc tgaaaagaac aataactgga cgtttaatct tgccaacgat
    19561 gacaacaaag acgcgaatgc aaagccgtcg gcatggtcgt ttcggctgga taatattctt
    19621 ttcgatcaag ggcggatcgc cattgatgac aaagtaagca aagcggatct ggaaattttt
    19681 gttgatccgt taggcaagcc gctgccattc agcgaagtta ctggatcgaa aggtaaagcg
    19741 gataaagaaa aggtgggcga ttacattttt ggcctgaagg cgcagggacg ttataacggt
    19801 gaaccgctca cgggtacggg aaaaataggc ggtatgctgg cgctgcgtgg cgaagggacg
    19861 ccgtttccgg tacaggctga tttccgttca ggtaataccc gtgttgcttt tgatggcgtc
    19921 gtgaatgacc caatgaagat gggcggtgtc gatttacggc ttaaattttc tggcgattca
    19981 ctgggtgatc tctatgaact gacgggcgtt ctgctgcccg ataccccgcc gtttgaaacg
    20041 gatggtcggc tggtagcgaa aatcgacact gaaaaatcgt cggtctttga ttatcgcggt
    20101 tttaatgggc gaattggcga tagcgatatc cacggttctc tggtctacac caccggaaag
    20161 ccacgaccaa aactggaagg tgatgtcgag tcgcggcaat tgcggctggc ggacctggga
    20221 ccgttgattg gcgttgattc cgggaaaggg gcagaaaagt cgaaacggtc tgaacagaag
    20281 aagggcgaaa aaagcgttca gcctgcgggc aaagtgctgc cttatgaccg cttcgaaacc
    20341 gataaatggg acgttatgga tgccgatgtt cgcttcaaag ggcggcgcat tgagcatggc
    20401 agtagcctgc cgattagcga tctttctact catatcatcc tcaaaaatgc tgacctgcgc
    20461 ctgcaaccgc tgaaatttgg catggcgggc ggcagcattg cggcgaatat tcatctggaa
    20521 ggcgataaaa agccgatgca ggggcgggca gatattcagg ctcgtcgact gaaactgaaa
    20581 gaactgatgc ccgatgtgga actgatgcag aagacgctgg gggaaatgaa cggtgacgcg
    20641 gaactacgcg gtagcggtaa ctcggtggcg gcgcttttag gcaacagtaa cggcaacctg
    20701 aaactgttga tgaatgacgg gctggtgagc cgcaacctga tggagattgt tgggctgaat
    20761 gtcggcaact acattgtcgg tgcgatattt ggtgacgatg aggtgcgggt gaactgcgcg
    20821 gcggcgaatc tgaatattgc caacggcgtg gcgcgcccgc agatttttgc tttcgatact
    20881 gagaacgcgt tgattaatgt taccggcacg gcaagttttg cttcggaaca gctggatttg
    20941 actattgatc cggagagtaa agggattcgg attatcacac tgcgttcgcc gctgtatgtg
    21001 cgtgggacgt ttaaaaatcc gcaggctggg gtgaaagccg gaccgctgat tgcccgtggt
    21061 gctgttgctg cggcactggc aacgctggta acaccggcgg cggcgttact ggcactgatc
    21121 tcaccttccg aaggggaggc taatcagtgt cggacgatat tgtcgcagat gaagaagtga
    21181 tgataattgc cggatgtctg acatccggca attaaacaga tttagatttt tggcaaagtg
    21241 tgctttgttc atgccggatg cggcgtgaac gccttatccg gcctacaaat ccttgtgaat
    21301 tcaatatatt gtatggaaat tcagtcctga taagcgtagc gcatcaggct tttcacactt
    21361 attacaacga ctgatgtcgc gtctcatggg tcagcagcag ggcgattaac gtcaagccag
    21421 ccatcgccgc cagatataac cccaccgcac ctaacccgta gttagcctgc aaccaggctg
    21481 cgatatacgg cgcaacggaa gccccgagaa tcgacgatac gttgtaagag aacgatgctc
    21541 cggtgtaacg cacttctgtc ggaaacagct ctggtaacag cgcacccatc ggcccgaagg
    21601 tcagccccat cagacttaac cccagcagca ggaaggcaaa aaccagaatc gggttgccag
    21661 aaccgagcag cgggttaaag gcgaacagcg cgaacaggat gatcagcgtg gtgatgatca
    21721 ccatgctttt acggcgacca aaggcatcag ccagtaatcc ggcgactggc accatcacgc
    21781 caaaaccaat aactgccatc atcagcatcc acaacacttc gttacgcggc aggccaagcc
    21841 caactggcgc ggcggcggta ctgaaggtca tagagtagac cgtcatgatg taaaacagcg
    21901 tataggttgc cagcataatg aacgtaccca gtacggttac gcgaacatgt ttggtcagca
    21961 gcgtacccag cgggatcttc acctgttttt tcgctttagc gactttctca aacaccggcg
    22021 actcatgcag cgacacgcga acatacaggc cgataatgac cagcaccgcc gagaagataa
    22081 atggcacacg ccagccccag ctcataaact gctcatcggt cagtagccag gaaagcagca
    22141 agaaagtgcc gttggcaaag aagaagccaa tcggtgcgcc tagctgtggg aaggagccat
    22201 acagtgcacg tttgcgcggt ggggcgtttt cagtcgccag cagcgccgcg ccgccccatt
    22261 caccgcctaa gcccagaccc tgaccaaatc gagccagcgc cagcagcagt ggggcgaaaa
    22321 taccaatcgt ggcatagccc ggcagcagac caatcaccac ggtcgaaatc cccatcgtta
    22381 gcaacgaggc gaccagcgtc gctttacgcc caacgcgatc gccaaaatga ccaaaaacgg
    22441 cagagccaat ggggcgcgcg acgaaggcga tggcgaaggt ggcgagcgac tgtagcgttg
    22501 ctgccgttgg atcgccctgc gggaagaaga tatgcggaaa cacaataacg gccgcagtgg
    22561 cgtaaatgta aaagtcgaag aactcaatgg ccgtgccaat gagagaggcg acaaggactt
    22621 tattacgcga gttgatcggc gtgtattctt gttcgtggtc gagtgttgtg gctgttgctt
    22681 gcataatctt ttcttatttt ttggctaacg aatagccata ttacgcacag caaaagtgat
    22741 atttcaatgt gtaacaaatc gcggaaatgg tcaaaaacca ggcaaaaagg ggataatgtt
    22801 taggccagtg atttttcttc tatgaaatgc ttcacataat tttataaata gtggataaaa
    22861 ctggtttcag gttaaataaa agttaccaac tggatttata tgctggggtt atgagttgga
    22921 gtgaaattgt ttgctcccaa atagatggct gaagaggctc tggggaagag tttcaagagg
    22981 ttatgtgcgc attatagccg gatgcgatgc tggcgcatct tatccggcct acggggagcc
    23041 aggccgaata aaatcgtatg ccggataagg cgtttacgcc gcatccggcc tattttaagg
    23101 ctgcgttttc cctggcattc gcgggtcgtc tttatattcg gcggtggcaa tccacgccgc
    23161 gcaaaacagc gtcaaacggg cgaagaagta gaaaaatgcc atcagcccca gcacggaacc
    23221 aaatgctgcg ccagaggggg atttcatcag cgatggcagg gtgtaggtca tcacgatttt
    23281 aatcacttca aaaccaatag cagccagaaa tgttccgcga atcagggctt ttttgcgcgg
    23341 acggtggcgc ggcaaccgcc agaagatcca gaagaaaagc agatagttgg cgaagatgga
    23401 aatcgccagg ccaatcaatc gccacgtcgg tttcagccac tcaatgctgt tcaggtgcag
    23461 ggcgctaata atcatttgct gcgccgaacc ggcaaccgag gtgatcgaaa gcgtcacaat
    23521 cagcgcaatc aacaaaccaa tcagtgaaat aaaatcacgc agatatttta cccagaactt
    23581 ctcctgatct tgcggcgagc gttcccagac atcgcgcgac tgggcacgaa tcgcttcacg
    23641 caggttaccc atccagttga tgccggaata aagtgccacc gccaggccga caagccctac
    23701 agtcgtacgc tgctgaacgg cggtgttgat ggtgtttttc aacgtggcgg ctagtgtcgg
    23761 atcgctgatg ttttgcagaa ttttgtcgaa gatatcctgt agcaacatcg gatgggaggc
    23821 cagcacaaag ccccctgcgg caaacgacac catcaaaatc gggatcatcg acaaaaatga
    23881 gaaataggtg atagccgcac caaactggtt acccagccga tcgttaaagc gttctgtcgc
    23941 gcgaataagg tgcgcaataa ccggttggcg ttggactttt tcggcagtgg tggtgacggt
    24001 ttccagcgcc tggctaactt tgccgccttt ctcgctatta tccaactgct taatcggctc
    24061 gtgctccaga tcctgggtgg gacgtttgat ctcgttttcc tgcgtcatcc gctcttttgt
    24121 ccttctgtcg tggtcattct taaaaagtat agtcggtcag tccaggtgct ctttcattac
    24181 gccagccagc cactgcataa acaggtttac acgccgggaa agctcccgac gctgcgggta
    24241 aaccagcgaa agggagagcg gctcggcacg gtagcctggc aacacttcaa taagccgccc
    24301 ggcacgcagg gcttcgcgca cggcaatgcg cgggatctga ataatcccca gaccggcaag
    24361 accggcggcg agatagtttt cgctactgtt taccgtcaac atgccgccag acttaaacca
    24421 ctggacgcca ttaacgctgg caacctcaaa acctaacgga tgtacaccca ggtgcggtgt
    24481 ataacgcact attgcgtgtg aagtcagatc atcggggctt tgcggataac caaagcgcgt
    24541 cagatagtgc ggactggcac agttgaccat ggttagtttg ccgaggggac gggcgataac
    24601 gccatcttcc ggtaacgcgc cagtgcgtat cacgcaatca aaaccatcat gaagaatatc
    24661 taccggacgg ttatggctac tcagttccag ctcaattccc ggatgctgat agagaaattc
    24721 cgacagacgc ggcagtaaca ggtttttcgc gattcctgac gggatgtcga tgcgtaattt
    24781 accgctgata ctggtggcat cctgttgaaa cagaccgtcc agttcgctga gattactcaa
    24841 cacatctttt gcccgttgat aataggtcat gccttctggc gtgagtttga cccgtcgcgt
    24901 agtgcgctgg agaagctggg tgccaagttg atgttccagt gcctgtatct ggcgcgaaac
    24961 acttcccttt ggcaaagcaa agaaatccgc cgcgcgggaa aaactttcca gctccgcgac
    25021 tttgatgaac aactgcattg cgtgaatttt atccatttct ttgaccgatt gttgttcaca
    25081 gcgaaacagt aacgcgcata tcgcattgtt tattgttttt tctaacatct aatgccgggt
    25141 ttcacaatgt tgtcatgacg aatgtggcga tttctcagca ggaaaagggc ggaatagctt
    25201 tatgagaaag gtgaatcccc ttaaacagag cgtatttttc agtgattccc tttaaagtga
    25261 atagaggacg tattggctat ttgtgcttat ttttgtctca ttgccatttg ttattttggc
    25321 ttgtagtcgc ggaatttcat ttttaattgc atgcgtacta accaccaata taaggaaatc
    25381 ctcaaaattg ttttaaatag tgccctcgac atgtaatttc aggcgtttag ctgtaactat
    25441 ttatatcggt aactattttc tacgcgtcat ctctgtagaa tggccaccgc caaatggcga
    25501 attcttattt tttctcgcgg gtaataccct tccactactt aagtcttttt aacgtattca
    25561 ggtggagatt cagttatgca aatagtcatg tttgacaggc agtcaatatt tattcatgga
    25621 ataaaaatca gtttacagca gcgtattcca ggagtgagta ttcagggggt cagtcaggca
    25681 gacgagttat ggcaaaagct ggaaagttac cctgaagcct tagttatgct cgatggcgat
    25741 caggatggtg agttttgcta ctggttgctg caaaaaaccg tggtgcagtt tcctgaggtt
    25801 aaggtgttaa tcacggcgac ggattgcaat aaacggtggt tacaggaagt tatccatttt
    25861 aatgtgctgg ccattgtgcc tcgcgattca accgtcgaga catttgcgtt ggcggtaaat
    25921 agtgcggcga tggggatgat gtttctgcct ggagactggc ggacgacgcc ggaaaaggac
    25981 attaaagatc tcaaatcgtt aagcgcccgc cagcggga
//
LOCUS       DAJTUI010000055        25821 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTUI010000055 DAJTUI010000000
VERSION     DAJTUI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.055"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..202)
                     /locus_tag="OO464_002415"
     CDS             complement(<1..202)
                     /locus_tag="OO464_002415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6227537.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTVFEPVNSFQKNQKTLAR"
     gene            complement(335..640)
                     /locus_tag="OO464_002416"
                     /pseudo
     CDS             complement(335..640)
                     /locus_tag="OO464_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            708..1323
                     /gene="tnpA"
                     /locus_tag="OO464_002417"
                     /pseudo
     CDS             708..1323
                     /gene="tnpA"
                     /locus_tag="OO464_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1651..2733
                     /locus_tag="OO464_002418"
     CDS             1651..2733
                     /locus_tag="OO464_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR6227538.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3222..4020)
                     /locus_tag="OO464_002419"
                     /pseudo
     CDS             complement(3222..4020)
                     /locus_tag="OO464_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(4030..4581)
                     /gene="ybcL"
                     /locus_tag="OO464_002420"
     CDS             complement(4030..4581)
                     /gene="ybcL"
                     /locus_tag="OO464_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR6227539.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(4750..5082)
                     /gene="emrE"
                     /locus_tag="OO464_002421"
     CDS             complement(4750..5082)
                     /gene="emrE"
                     /locus_tag="OO464_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR6227540.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(5427..5741)
                     /gene="fliE"
                     /locus_tag="OO464_002422"
     CDS             complement(5427..5741)
                     /gene="fliE"
                     /locus_tag="OO464_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR6227541.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            5955..7614
                     /gene="fliF"
                     /locus_tag="OO464_002423"
                     /pseudo
     CDS             5955..7614
                     /gene="fliF"
                     /locus_tag="OO464_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            7607..8602
                     /gene="fliG"
                     /locus_tag="OO464_002424"
     CDS             7607..8602
                     /gene="fliG"
                     /locus_tag="OO464_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6227542.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            8595..9281
                     /gene="fliH"
                     /locus_tag="OO464_002425"
     CDS             8595..9281
                     /gene="fliH"
                     /locus_tag="OO464_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6227543.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            9281..10654
                     /gene="fliI"
                     /locus_tag="OO464_002426"
     CDS             9281..10654
                     /gene="fliI"
                     /locus_tag="OO464_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR6227544.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            10676..11017
                     /gene="fliJ"
                     /locus_tag="OO464_002427"
                     /pseudo
     CDS             10676..11017
                     /gene="fliJ"
                     /locus_tag="OO464_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            11014..12141
                     /gene="fliK"
                     /locus_tag="OO464_002428"
     CDS             11014..12141
                     /gene="fliK"
                     /locus_tag="OO464_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6227545.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            12246..12710
                     /gene="fliL"
                     /locus_tag="OO464_002429"
     CDS             12246..12710
                     /gene="fliL"
                     /locus_tag="OO464_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6227546.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            12715..13719
                     /gene="fliM"
                     /locus_tag="OO464_002430"
     CDS             12715..13719
                     /gene="fliM"
                     /locus_tag="OO464_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6227547.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            13716..14129
                     /gene="fliN"
                     /locus_tag="OO464_002431"
     CDS             13716..14129
                     /gene="fliN"
                     /locus_tag="OO464_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6227548.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            14132..14497
                     /gene="fliO"
                     /locus_tag="OO464_002432"
     CDS             14132..14497
                     /gene="fliO"
                     /locus_tag="OO464_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR6227549.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            14497..15234
                     /gene="fliP"
                     /locus_tag="OO464_002433"
     CDS             14497..15234
                     /gene="fliP"
                     /locus_tag="OO464_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR6227550.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            15244..15513
                     /gene="fliQ"
                     /locus_tag="OO464_002434"
     CDS             15244..15513
                     /gene="fliQ"
                     /locus_tag="OO464_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6227551.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            15522..16307
                     /gene="fliR"
                     /locus_tag="OO464_002435"
     CDS             15522..16307
                     /gene="fliR"
                     /locus_tag="OO464_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR6227552.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            16592..17215
                     /gene="rcsA"
                     /locus_tag="OO464_002436"
     CDS             16592..17215
                     /gene="rcsA"
                     /locus_tag="OO464_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR6227553.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(17259..17447)
                     /gene="dsrB"
                     /locus_tag="OO464_002437"
     CDS             complement(17259..17447)
                     /gene="dsrB"
                     /locus_tag="OO464_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6227554.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            17610..17837
                     /gene="yodD"
                     /locus_tag="OO464_002438"
     CDS             17610..17837
                     /gene="yodD"
                     /locus_tag="OO464_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR6227555.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            18135..18950
                     /locus_tag="OO464_002439"
                     /pseudo
     CDS             18135..18950
                     /locus_tag="OO464_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(18947..20641)
                     /gene="yedQ"
                     /locus_tag="OO464_002440"
                     /pseudo
     CDS             complement(18947..20641)
                     /gene="yedQ"
                     /locus_tag="OO464_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(20812..20994)
                     /locus_tag="OO464_002441"
     CDS             complement(20812..20994)
                     /locus_tag="OO464_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR6227556.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(21073..21990)
                     /locus_tag="OO464_002442"
     CDS             complement(21073..21990)
                     /locus_tag="OO464_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR6227557.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            22163..23083
                     /gene="yedA"
                     /locus_tag="OO464_002443"
     CDS             22163..23083
                     /gene="yedA"
                     /locus_tag="OO464_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR6227558.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(23072..23542)
                     /gene="vsr"
                     /locus_tag="OO464_002444"
     CDS             complement(23072..23542)
                     /gene="vsr"
                     /locus_tag="OO464_002444"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6227559.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(23523..24941)
                     /gene="dcm"
                     /locus_tag="OO464_002445"
     CDS             complement(23523..24941)
                     /gene="dcm"
                     /locus_tag="OO464_002445"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6227560.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(25008..25548)
                     /locus_tag="OO464_002446"
                     /pseudo
     CDS             complement(25008..25548)
                     /locus_tag="OO464_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(25588..>25821)
                     /locus_tag="OO464_002447"
     CDS             complement(25588..>25821)
                     /locus_tag="OO464_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227561.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6510 a         6619 c         6361 g         6331 t
ORIGIN      
        1 gtctcgccag cgtcttctgg tttttctgaa aactgtttac aggctcaaag actgtgccat
       61 ctgacagcga ggcaagttta gccacgccag catccagccc gaccattgat gctgaagggt
      121 gaaccggagt tgatacttca ctttctgcct gaatactgat gtaccactta ccgcaggact
      181 ggctgacagt gacatttttc acaacacccg tgacctgacg gctgttccgg tagcgcatcc
      241 agccaagttt tggcagaaaa atacggctgt tttcctgagc gagcttaacg ccctgcggat
      301 agcggaatgc atcattctgt caccgctttt tgaatcaggg gaacgccgag cgcttctgga
      361 aaaagttttt gcaggcctgc tcagggtctt tcagtgactg ttgcaatggc tgtgacggag
      421 catctttaat ccattgcgtt tcagtggcat ttttccactc aaccaatcag gaagccattt
      481 tcgcgtaagg gatgtctttg ttaccggcct tatgattctc attctgacgt gccagagtac
      541 gactgaaaac gaaacgacat gcgcctgcga agcgcctcat ttcacgcgcc tgttgaccac
      601 cgggtcttaa ccggaatttg aatacctgta gtcgtttcac agggcaaatt atactttggt
      661 ctgtgaaaaa aactgatatc cgacgcggaa ggcactgtgt tttcctgatg catgctcacc
      721 gggtctttgt taccaagtac cgacgccaga tttttgacca tgacgcgaca gaaaaactac
      781 gcacttactt ttcaaatgta tgtgcttatt ttgaagctga actggttgaa atggatggcg
      841 aaccagatca cgtccatctg ttaattaact atccccccaa actggcgata tccagtctgg
      901 taaacaaaca gcctcaaagg cgtatcgggt aggttactgc gacgggatcg accagatatt
      961 gctgccaggt attaccgcaa agatgttttg tggcgtcccg gctattttgc cagtagctgc
     1021 ggaggtgcgc caatatccgt catccgccaa tacattgaac aacagcaaac acctggtcag
     1081 atgaaaaaac gagccttata tccccgccct gaaggacggg cttttacggt gcactggata
     1141 agcctggaga ttatctacac ctgcatggac atcattgtac tatgccccgt acatgctcca
     1201 tcacatcctc aacatcaaag tattagagcc ttgcaaacca ttatgcgtct cagtttgtct
     1261 catctatctt acgcaaaaaa aatagaacta atatcaagcg gcgctatatt gcgtattaaa
     1321 taaaaccacc tcaattacat taataaatct aaataaatat catgcaaata atttccatgg
     1381 ttatttttag gctatttaaa ttaaaaaaat aaattatgag tcaatcataa ataacaacct
     1441 ttaatataat ttgactacca aatttatatt cgttcacaaa atagtcatgc attgtgtgca
     1501 atagaaacag ttcagataaa aatagggatc tacttcacaa tttaaatgag aaactaaaac
     1561 ttacatcttg aaataatctc attgattgga tgaatatttg tagtgcagtg acatcatttt
     1621 taaataatag ttcaaataaa agggctcacg atgaaaaaat taacagtggc aatttctgct
     1681 gtagctgcat cagtactgat ggcgatgtct gctcaggcag cagagattta caataaagac
     1741 agtaacaagc tggatctgta cggaaaagtt aatgctaagc actacttctc ttctaacgat
     1801 gcagatgatg gcgacacaac ttatgttcgt ctgggcttca aaggcgaaac tcaaatcaac
     1861 gatcaactga ctggtttcgg tcagtgggaa tatgaattca aaggcaaccg tgctgaatct
     1921 caaggttctt ccaaagataa aacccgtctt gccttcgctg gcctgaaatt cggtgactac
     1981 ggctccatcg attatggccg taactacggt gtagcatacg atatcggtgc atggaccgac
     2041 gttctgccag aattcggtgg cgatacctgg acccaaacag atgtgttcat gactggtcgc
     2101 accacaggtg ttgcaaccta tcgtaacaac gacttctttg gtctggttga tggtctgaac
     2161 tttgctgctc agtatcaggg taaaaatgac cgcactgacg taactgaagc taacggtgat
     2221 ggtttcggtt tctccactac ttatgagtat gaaggattcg gtgtaggtgc aacctatgct
     2281 aaatctgacc gcactaatga tcaggttatc tacggtaaca acagcctgaa tgcatctggt
     2341 caaaatgctg aagtatgggc agctggtctg aaatatgatg cgaacaacat ctatctggct
     2401 accacctatt ctgaaaccca gaacatgact gtttttggta ataaccatat tgccaacaaa
     2461 gcacaaaact tcgaagtagt tgcacaatat cagttcgact tcggtctgcg tccgtccgtt
     2521 gcttacctgc aatctaaagg aaaagacttg ggtgcgtggg gtgatcagga cctgattgaa
     2581 tatattgatg taggtgcaac ctattacttc aacaaaaata tgtccacttt tgttgattac
     2641 aaaatcaacc tgattgataa gagcgatttc acgaaagcat ctggcgttgc taccgatgat
     2701 atcgttgctg taggattggt ttaccagttc taatctgatt actcaaagat atgttgtggg
     2761 aggctttgcc tccccaacat ataagtggcg ctctcaagcc actttcctta gaagcactat
     2821 cttgcttctg actatataaa ttacccttta ttttctggga cgtttcaacg ccccattttt
     2881 aataatactc agtgaacaat taacgtatca attagattta ataacaacga tatccgtacc
     2941 taacctgata tctaatgtca taagcatccc tttaattatc ccctcaacct cttgtaaatt
     3001 tcccactctt gagctccaca tagcccaaac gccggatgca aaaaaaatca tacgtgcatc
     3061 tctgaaattg tgatggagga agtgagtatc aattatatgt gcttataaac gttcccgatt
     3121 ggcacagaag tcaccggatt gttcaggctc caataggttg agtatgtcgt atttattttc
     3181 caaaataaaa atcgatgctg ttgctcctta ataaaatact tttactctct ggacgctttt
     3241 cttagctctc ttggtgttat attaaaacga ttatggaacc tttcagtaaa acgagaagga
     3301 cacttgtaac cattttccct ggcaatctcg ctgatgggtt ttactgtcgt ttgtatagca
     3361 ggacaacgca ttatttaacc tcacgtcgtc caatatactt tgaaaactta ttccctcgct
     3421 tgccagacgg cgatgcaatg tggaaacaga aatgtagaga tatctcgcta ccttgtttgc
     3481 tgtccatttt gttctaggtt cggatagcat taggttataa caacgactta ttaatgattg
     3541 tttactatct gataaaagta aacaattaac atgatgtgct cctaatgaaa gtagaacgcc
     3601 cattgctaag tgttcctgaa tttgcgttga gaagcctcgg gaaatagatg tttttagttg
     3661 ctcccaacaa tatattaact cggggttctg aggtaagcag aaacctgtcg tgttacgaat
     3721 ttgatcagtt atcgcataaa atttttgaaa cttttcaatt agatcaatgg gataataaag
     3781 catttctgca agataaagac ctgcttctgg ataattcaca atataaaatt catatccaca
     3841 gggaaataat attatctgat tattatcaac agttagacta tgcgtctccc aattgataac
     3901 tttttttccc tgacggatac gacataaagc tggcataaga ggcttaaccc tgtgaatctc
     3961 atgatgttta tgcatccgta tttcttcaat ctttaggtta gacttatctc ttgccagcat
     4021 actcttaccc tattttatct cataaactgg tgttatctca gcggttgcaa ttttattggc
     4081 attaagcata taaccaacta acgctccgct ggaattagaa tccacaggaa tcttatcagt
     4141 ttttagagcc catactttaa actggtaatg atggggttta tctcctttgg gaggacatgc
     4201 tccaccaaat cctgcataac caaaatcatt tcggccttga acagcaccag tcggaagttt
     4261 tgtaccatca cgtcttcctg catcagtggg caaatatgtt acagttgctg gaatattagc
     4321 aacagtccag tgccaccatc cactgcctgt aggagcatct ggatcataca cagttacggc
     4381 aaagcttttg gtgccttcag gagcaccaga ccaggttaat gagggcgatg tattaccacc
     4441 ttcacaccca aatccagaaa atacatgaga cgttgtaagt tgctctcctg tttttatttc
     4501 attactagtg acctgaaatg ctgcggcctg cgcagaaaat gttatgaatg ccaatacact
     4561 tgaaacgata agttttttca taaaaacctc attgttgtga cctatcgtta ttttatttga
     4621 tgtgccttga tctcattatg cataaaggcg caatgatcgc gcaaatgcaa tcataattat
     4681 aaaggtgccc cgaacattag ccataatatg cacaaatcag attcctgtta tctcaaggag
     4741 aattattttt taatgcggtg cacttcgtga caacaagtta atgactaaca caccggcaca
     4801 aatcaacatc atgcctatga tagctggcag gtccagtcgt tgaccaaaaa acccccagga
     4861 aagcaaacta atcagaacga taccgactcc tgaccagata gcataagcaa tccctgtagg
     4921 tatataagcc agcgtctgag ctaataacca gaatgatgca caatagcaaa taattgtgcc
     4981 aacagagggc cataaccgtg taaacccctc tgaaaacttc attaaggttg taccaatgac
     5041 ctctgcaagt attgcgccac caagataaat ataagggttc atagcatatt ctttcctgtt
     5101 catactggag agaattgtac tacagtttga acacaactca ccgttttcat cctggtgtat
     5161 gtgtatccaa ggggctctgg cgtggcaaga gataaaggca ggaacaataa tgatcagaag
     5221 gaaccccagg ctaacgaatg agttcactct tcctgggagc aaagcgaaga caaaacatgt
     5281 tatcgcgttt acggatcgtg gatggcagaa aacaaccagg atcaggtatc cattttaaac
     5341 cagaaattga gtgagtttgc cccatccatg ccccacgctg tgggatcgga tataattaaa
     5401 caagcatagt caacaggtta cacaacctac acctgcatgg acatcacttc ctgatacgcc
     5461 gccaccagct tattacgcac ctgaatcccc atttgcatag aaactgaggc tttttgcata
     5521 tcggtcatca catcgtttag cgccacgccg ggttcaccga gggtgaattt ttctgcctgc
     5581 gtgcgggcga ctgtttgtgt atcgcttatg cgatcgagcg cggcgtgcag ctgcccggca
     5641 aaactaatgg tcggttgcgg cagtgattcc tgcgcacgcg cactcatcgc cgtagcctgt
     5701 aactggctga taaccccttc aatcccctgt atcgctgaca ttttcgtctc ccggataatt
     5761 tctggtaaca aagcctacca gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa
     5821 actggtttat tggcggatag aaaaaacgaa agcacaaata atgggagcgt caatttttcg
     5881 agtgtgctga cccgagagtg agtcttgttc cactttgcca ataacgccgt ccataatcag
     5941 ccacgaagtg cgcgatgaat gcgactgcag cccagacaaa atctcttgag tggcttaatc
     6001 gcctgcgtgc gaatccgaaa attccattga ttgttaccgg ttccgcggca gtggcggtca
     6061 tggtcgcact gatcctgtgg gcgaaagccc ccgactaccg cacattattc agcaatcttt
     6121 ccgatcagga tggtggcgca attgtcagcc aactgacgca aatgaatatt ccttaccgct
     6181 tcagcgaagc cagcggcgct attgaggttc cggcagataa agttcacgaa ctgcgcctgg
     6241 cgcaacaagg tttgcccaaa ggcggcgcgg tcggtttcga actgctcgat caggaaaagt
     6301 ttggtatcag ccagttcagc gaacaggtga attatcagcg ggcgctggaa ggcgagcttt
     6361 ctcgtaccat cgaaactatc ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga
     6421 aaccgtcttt attcgtccgt gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt
     6481 tacccggccg cgcactcgat gaagggcaaa ttagcgctat tgtacatctg gtttccagcg
     6541 cggttgctgg tctgccgcct ggaaacgtca cgctggtgga tcagggcggg catctgttaa
     6601 cccagtccaa taccagcggg cgcgatctta atgacgctca gttgaaatat gccagcgatg
     6661 tcgaaggccg tattcagcag cgtattgaag cgatcctgtc acctattgtt ggtaacggta
     6721 atattcacgc ctaggtcacg gcgcaactgg acttcgccag taaagaacaa acggaagaac
     6781 agtatcgccc taacggtgat gaatctcatg cggcgcttcg ttcacgccag cttaatgaga
     6841 gcgagcaaag cggttccggt tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg
     6901 cacctgcgaa taacgcgcca atcagcacgc ctccgacaaa tcaaaataac cgccagcagc
     6961 aggcgagcac caccagcaat agtgggccgc gtagcacaca gcggaatgaa accagtaact
     7021 acgaagtcga tcgcaccatt cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt
     7081 cagtcgcggt agtggtgaat tacaaaacct tgccagacgg taaaccattg cctctcagca
     7141 acgaacagat gaagcaaatt gaagctctga cccgcgaggc gatgggcttt tctgaaaaac
     7201 gcggtgattc gctcaatgtt gttaactcgc cgttcaatag cagtgacgaa agcggcggag
     7261 cactgccgtt ttggcaacag caagtgttta tcgatcagtt gctggctgcc ggtcgctggt
     7321 tgctggttgc tggtactgct ggtggcgtgg ttgctgtggc ggaaagcggt acgcccgcag
     7381 ctaacacgtc gcgccgaggc ggtgaagact gtgcagcaac aggcgcaggc ccgcgaggaa
     7441 gtggaagatg cggtggaagt ccgtctgagc aaagacgaac aactccaaca acggcgcgct
     7501 aaccaacgac tgggggcaga agtcatgagc cagcgtatcc gtgaaatgtc tgataacgat
     7561 ccgcgcgtgg tggcgctggt cattcgccag tggataaata acgatcatga gtaacctgac
     7621 aggcaccgat aaaagcgtca tcctgctgat gaccattggc gaagaccggg cggcagaggt
     7681 gttcaagcac ctctcccagc gcgaagtgca aaccctgagc gctgcaatgg cgaacgtcac
     7741 gcagatctcc aacaagcagc taaccgatgt gctggcggag tttgagcaag aagctgaaca
     7801 gtttgctgca ctgaatatca acgccaacga ttatctgcgt tcggtattgg tcaaagctct
     7861 gggtgaagaa cgtgccgcca gcctgctgga agatattctc gaaacccgcg ataccgccag
     7921 cggtattgaa acgctcaact ttatggagcc gcagagcgcc gccgatctga ttcgcgatga
     7981 gcatccgcaa attatcgcca ccattctggt gcatctgagg cgcgcccagg ccgccgatat
     8041 tctggcgttg ttcgatgaac gtctgcgcca cgacgtgatg ttgcgtatcg ccacctttgg
     8101 cggcgtgcag ccagccgcgc tggccgaact gaccgaagta ctgaatggct tgctcgacgg
     8161 tcagaatctc aagcgcagca aaatgggcgg cgtgagaacg gcagccgaaa ttatcaacct
     8221 gatgaaaacg cagcaggaag aagccgttat taccgccgtg cgtgaattcg acggcgagct
     8281 ggcgcagaaa atcatcgacg agatgttcct gttcgagaat ctggtggatg tcgatgatcg
     8341 cagcattcag cgtctgttgc aggaagtgga ttccgaatcg ctgctgatcg cgctgaaagg
     8401 ggccgagcag ccactgcgcg agaaattcct gcgcaatatg tcgcagcgtg ccgccgatat
     8461 tctgcgcgac gatctcgcca agcgtggtcc ggtgcgtctg tcgcaggtcg aaaacgaaca
     8521 gaaagcgatt ctgctgattg tgcgccgtct tgccgaaact ggcgagatgg tgattggcag
     8581 cggcgaggat acctatgtct gataatctgc cgtggaaaac ctggacgccg gacgatctcg
     8641 cgccaccacc ggcagagttt gtgccaatgg tcgagtcgga agaaaccatt attgaagagg
     8701 ccgaacccag ccttgagcag caactggcgc aactgcaaat gcaggcccac gagcagggtt
     8761 atcaggcggg gattgccgaa ggtcgccagc aaggtcacga acagggctat caggaaggac
     8821 tggcccaggg gctggaacaa ggtctggcag aggcgaaggc tcaacaagcg ccaattcatg
     8881 cccggatgca gcaactggtc agcgaatttc aaactaccct tgatgcgctt gatagtgtga
     8941 tagcttcgcg cctgatgcag atggcgctgg aggcagcacg tcaggtgatc ggtcagacac
     9001 caacggtgga taactcggca ctgatcaaac agatccaaca gttgttgcag caagaaccgt
     9061 tattcagcgg gaaaccacag ctgcgcgtac acccggatga tctgcaacgt gtggatgata
     9121 tgctcggcgc aactttaagt ttgcatggct ggcgcttgcg gggcgatccc accctccacc
     9181 ctggcggatg taaagtctcc gccgatgaag gcgatctcga cgccagtgtc gccactcgct
     9241 ggcaagaact ctgccgtctg gcagcaccag gagtggtgta atgaccacgc gcctgactcg
     9301 ctggctaacc acgctggata actttgaagc caaaatggcg cagttgcctg cggtacgtcg
     9361 ctacgggcga ttaacccgcg ctaccgggct ggtgctggaa gccaccggat tacaattgcc
     9421 gctcggcgca acctgtgtca ttgagcgcca gaacggcagc gaaacgcacg aagtagaaag
     9481 cgaagtcgtt ggctttaacg gtcaacggct gtttttaatg ccgctggagg aagtggaagg
     9541 tgtcctgccc ggcgcgcgtg tatatgccaa aaacatttcg gcagaagggc tgcaaagcgg
     9601 caagcagttg ccgctcggtc cggcgttatt aggtcgtgtt ctggacggta gcggtaaacc
     9661 gctcgatggc ctgcccttcc ccgatacgac ggaaaccggt gcgctgatta ccccgccatt
     9721 taacccgttg caacgtacac cgattgaaca tgtgctggac accggcgtgc gcccaatcaa
     9781 tgccctgctt accgtcggac gcgggcagcg tatggggctg tttgccgggt cgggcgttgg
     9841 taaaagtgtg ctgctgggga tgatggcacg ctacacccgc gccgatgtca ttgtcgtggg
     9901 tttgattggt gaacgtgggc gcgaagtaaa agattttatt gagaatatcc tcggtgccga
     9961 aggacgtgca cgctcagtgg tgattgccgc tccggcggat gtttctccgc tcctgcgaat
    10021 gcaaggtgcc gcctatgcca cgcgcattgc cgaagatttt cgcgatcgtg gtcagcatgt
    10081 gttgctgatt atggactccc tcacccgcta cgcgatggcc cagcgtgaaa ttgcgctggc
    10141 gattggcgaa ccacctgcca ctaaaggtta tccaccgtcg gtgtttgcca aattaccggc
    10201 actggtcgag cgtgccggta atggcattag cggcggcggc tcgattaccg cgttttatac
    10261 cgtgctcacc gaaggcgatg accagcagga ccccattgct gactccgcgc gggcaatcct
    10321 cgacggtcac attgtgctgt ctcgccgact ggcggaagcc gggcactatc cggctatcga
    10381 tattgaagcg tcgatcagtc gcgcaatgac ggcgttgatc agtgagcaac attacgcgcg
    10441 agtgcgcacc ttcaaacagc tgttgtcgag ttttcagcgt aaccgcgatc tggttagcgt
    10501 cggcgcgtat gccaaaggca gcgatccgat gctcgataaa gccatcgccc tgtggccgca
    10561 gctggagggc tatttgcaac aaggcatttt tgaacgcgcg gactgggaag cgtctctcca
    10621 ggggctggag cgtattttcc cgacagtgtc ataacccagg agataacggc agatggcgga
    10681 agaacagctc aaaatgttga tcgattatca gaatgaatat cgcaataacc tcaatagcga
    10741 tatgagtgcc gggatgacca gcaaccgctg gatcaactat cagcagttta tccagacgct
    10801 ggaaaaagcc attactcagc atcgccagca acttaatcag tggacgcaga aagttgacat
    10861 tgccctgaac agctggcgag aaaagaaaca acgtttgcag gcctggcaga cactgcagga
    10921 acggcaatcc acggcggcac tgcttgcaga aaaccgcctc gatcagaaaa agatggatga
    10981 gttcgcccag cgcgccgcca tgaggaaacc tgaatgattc gcttagcgcc cttgattacc
    11041 gccgacgttg acaccaccac attgcctggc ggcaaagcca gcgatgctgc acaagatttt
    11101 ctcacgttgt tgagcgaagc attagcaggc gagacaacaa ctgacaaagc ggccccccag
    11161 ttgctggtag caacagataa gcccacgaca aaaggcgagc cgctggtcag cgatattctt
    11221 gccgatgcgc aacaagcgga tttactgatc cctgtggatg aaacacttcc tgtcatcaac
    11281 gacgaacaat ccacatcaac accgttaacc accgctcaga cgatgacgtt ggctgcggtg
    11341 gctgacaaaa atacgacaaa agacgaaaaa gcggatgatc ttaatgaaga cgtcaccgca
    11401 agtctgagcg ccctttttgc gatgttgccg ggttttgaca atacgcccaa agtgactgat
    11461 gcaccgtcaa ccgtgttacc ggcagagaaa ccaacgctct tcacaaaact gacttctgcg
    11521 caactcacta cagcacagcc tgatgacgcc cccggcacac cagctcagcc attaacaccg
    11581 ctggtagcag aagcccagag taaagcggaa gtcatcagca cgccttcgcc ggtgaccgct
    11641 gacgccagcc cgctaatcac tccacaccag acacagccac tgcccaccgt cgccgcgcct
    11701 gttttgagtg caccgctggg ttctcacgaa tggcaacaat cattgagcca gcatatttcg
    11761 ctgttcaccc gccaggggca acaaagtgca gagttgcgtc tgcacccaca ggatttaggt
    11821 gaagtgcaaa tttccctcaa agtggatgat aaccaggcgc agatccagat gatttcaccg
    11881 catcagcacg tacgcgccgc cctggaagca gcgctgccgg tactgcgtac gcagctggcc
    11941 gaaagtggca ttcagttagg gcaaagcaac attagtggcg aaagctttag tggtcagcag
    12001 caggccgctt cccagcaaca gcaaagccaa cgcacagcaa accatgaacc tctggcgggg
    12061 gaagacgacg atacgcttcc ggttcccgtc tctttacaag ggcgtgtaac aggtaacagc
    12121 ggcgttgata ttttcgccta acgtcagagg tagcaccgta atccgcgtct tttccccgct
    12181 ttgttgcgct caagacgcag gataattagc cgataagcag tagcgacaca ggaagaccgc
    12241 aacacatgac tgattacgcg ataagcaaga aaagcaagcg atcgctttgg atcccgattc
    12301 tggtattcat taccctcgcg gcctgtgcca gcgcaggtta cagctactgg cattcgcatc
    12361 aggttgccgc tgacgacaaa gcgcagcaac gcgtcgtgcc ctccccggtc ttctatgcgc
    12421 tggatacctt tacggtcaat ttgggcgatg cggatcgcgt gctttatatc ggcataaccc
    12481 tgcgactgaa agatgaagca acccgctcgc ggctgagtga gtatttgccg gaagtccgta
    12541 gtcgcttgct gttactgttt tcacgtcagg atgctgccgt actggcgaca gaagaaggca
    12601 agaaaaacct gattgccgag attaaaacca cactttccac cccgcttgtt gccgggcaac
    12661 cgaaacagga tgtcaccgac gtgctgtata ccgcttttat tctgcgataa cgacatgggc
    12721 gatagtattc tttctcaagc tgaaattgat gcgctgttga atggtgacag cgaagtcaaa
    12781 gacgaaccga cagccagtat tagcggcgaa agtgacattc gtccgtacga tccgaatacc
    12841 caacgacggg ttgtgcgcga acgtttgcag gcgctggaaa tcattaatga gcgctttgcc
    12901 cgccattttc gtatggggct gttcaacctg ctgcgccgta gcccggacat taccgtcggg
    12961 gccatccgca ttcagccgta ccatgaattt gcccgcaacc tgccggtgcc gaccaacctg
    13021 aacctaatcc acctgaaacc gctacgcggc acagggctgg tagtgttctc gccgagtctg
    13081 gtgtttatcg ccgtggataa cctgtttggc ggcgatggac gcttcccgac caaagtggaa
    13141 ggtcgcgagt ttacccatac tgaacagcgc gtcatcaacc gtatgttgaa gctggcgctg
    13201 gaaggctata gcgacgcctg gaaagcgatt aatccgctgg aagttgagta cgtgcgttcg
    13261 gaaatgcagg tggaatttac caatatcacc acctcgccga acgacattgt ggttaacacg
    13321 ccgttccatg tggagattgg caacctgacc ggcgaattta atatctgcct gccattcagc
    13381 atgatcgagc cgctacggga attgttggtt aacccgccgc tggaaaactc gcgtaatgaa
    13441 gatcagaact ggcgcgataa cctggtgcgc caggtgcagc attcacagct ggaactggtg
    13501 gccaactttg ccgatatctc gctacgcctg tcgcagattt taaaactgaa acccggcgac
    13561 gtcctgccga tagaaaaacc cgatcgcatc atcgcccatg ttgacggcgt cccggttctg
    13621 accagtcagt acggtactct caacggtcag tatgcgttac ggatagaaca tttgattaac
    13681 ccgattttaa attctctgaa cgaggaacag cccaaatgag tgacatgaat aatccggccg
    13741 atgacaacaa cggcgcaatg gacgatctgt gggctgaagc gttgagcgag caaaaatcaa
    13801 ccagcgaaaa aagcgctgcc gacgcggtgt tccagcaatt tggcggtggt gatgtcagcg
    13861 gaacgttgca ggatatcgac ctgattatgg atattccggt caagctgacc gtcgagctgg
    13921 gccgtacgcg gatgaccatc aaagagctgt tgcgtctgac gcaagggtca gtcgtggcgc
    13981 tggacggtct ggcgggcgaa ccgctggata ttctgatcaa cggttattta atcgcccagg
    14041 gcgaagtggt ggttgttgcc gataaatatg gcgtgcggat caccgatatc attaccccgt
    14101 ccgagcggat gcgccgcctg agccgttagt gatgaataac catgctactg tgcaatcttc
    14161 cgcgccggtt tctgctgagc cactgctgca ggtgagcggc gcactcatcg ccattattgc
    14221 cctgatcctc gctgctgcct ggctggtaaa acggctggga tttgccccta aacgcactgg
    14281 cgttaacggt ctgaaaatta gcgccagtgc ttcgctgggc gcgcgtgaac gggtcgtggt
    14341 ggtcgatgtg gaagatgcac ggctggtgct cggcgttacc gcagggcaaa tcaatctgct
    14401 gcataaactt cccccttcag caccaacgga agagataccg cagaccgatt ttcagtcggt
    14461 catgaaaaat ttgcttaagc gtggcgggag atcctgatgc gtcgtttgtt ttctgtcgca
    14521 ccggtccttc tctggctgat tacgcccctc gccttcgcgc aactgccggg tatcaccagc
    14581 cagccgctac ctggcggtgg acaaagctgg tcgctcccgg tgcagacgct ggtgttcatc
    14641 acctcgttga cgttcattcc agcgatttta ctgatgatga ccagtttcac ccgcatcatt
    14701 attgtttttg gtttattgcg taacgcgctg ggaacgccct ccgcgccacc taaccaggta
    14761 ttgctggggc tggcactgtt tttgaccttt tttattatgt cgccagtgat cgacaaaatt
    14821 tatgtcgatg cgtaccagcc attcagcgaa gagaaaatct caatgcagga ggcgctggaa
    14881 aaaggggcgc agccgctgcg tgagtttatg ctgcgtcaga cccgcgaggc agatttaggg
    14941 ttgtttgcca gactggcgaa taccggcccg ttgcaggggc ctgaagctgt gccgatgcgc
    15001 attttgctcc cggcctacgt gaccagcgag ttgaaaaccg catttcagat aggcttcacg
    15061 attttcatcc cttttttgat tatcgacctg gtgattgcca gcgtgttgat ggcattgggg
    15121 atgatgatgg ttccccccgc caccattgct ctgcccttta aactgatgct gtttgtactg
    15181 gtggatggct ggcaattgct ggtcggttcg ctggcgcaga gcttttacag ctagagaggc
    15241 aaaatgacac ctgaatcggt catgatgatg ggaactgaag cgatgaaagt cgcgctggca
    15301 ctggcagccc cgctgttgct ggtagcgctg gtcacgggcc ttatcatcag tattttgcag
    15361 gcagccacgc agattaacga aatgacgctg tcgtttattc cgaaaatcat cgccgtattt
    15421 atcgccatca ttattgccgg accgtggatg ctcaatctgt tgctggatta cgtccgcacc
    15481 ttgttcacta acctgccgta tatcatcggg tagcccgtac aatgatgcag gagacaagcg
    15541 atcaatggct ttcctggtta agcctgtact tctggccgtt actgcgcgtg ctggcgctca
    15601 tttctaccgc gccaattctg agcgaacgca gcgtgccgaa acgggtaaaa ctgggtctgg
    15661 caatgatgat cacgttcgcc attgccccat cattacctgc caacgatgtt cctgtttttt
    15721 cgttctttgc tctgtggctg gccgtacagc agatcctgat cggcattgcg cttggtttta
    15781 ccatgcaatt tgcctttgcc gctgtgcgaa ccgctggcga aattatcggt ctgcaaatgg
    15841 ggctgtcatt tgcgacattt gtcgatcctg gcagccatct caatatgccc gttttagcgc
    15901 gcatcatgga tatgctggca ttactgctgt tcctgacatt taacggtcat ttatggttga
    15961 tttcactgct ggtcgatacc tttcacaccc tgccgattgg tagcgaaccg ttgaacagca
    16021 atgcgtttct ggcacccacc aaagcaggga gtttgatttt ccttaacggg ctgatgctgg
    16081 cgttaccgct cattactctg ttgctgaccc tgaatctggc attaggttta ctgaatcgta
    16141 tggccccgca attatccatt tttgttattg gatttccatt aactctgact gtcggcatct
    16201 ctttaatggc ggcattaatg ccgttaattg cgcctttttg cgaacattta ttcagtgaaa
    16261 tttttaattt gctggctgat attattagtg aattaccatt aatataattc cgtaacgttt
    16321 atcatgctac cctaaggatt atccgaaaaa taatatctgc gaacatcttc caggatactc
    16381 ctgtagcgaa atatttgttt taagctcact cacatatcgc aacatttact ttaagacaat
    16441 tccaggcaaa ttatacaaca ctttacggga tagtaagtcc gcctgaaaaa ttgcgagagt
    16501 ggcgcattag gtgacccatg ttgttccgtt tagtcatgat gaaatattca ggtaagggga
    16561 attatcgtta cgcattgagt gagggtatgc catgtcaacg attattatgg atttatgtag
    16621 ttacacccga ctaggtttaa ccgggtatct gttgagtaga ggggttaaaa aaagagaaat
    16681 caacgacatt gaaaccgttg atgaccttgc catagcttgt gattcacagc gtccttcagt
    16741 ggtgtttatt aatgaggact gtttcatcca cgatgcttct aacagtcagc gcatcaagca
    16801 catcattaat caacatccca atacgttatt tatcgttttt atggcaattg ccaatgttca
    16861 ttttgatgaa tatctattgg tcagaaaaaa tttattgatc agttctaaat cgattaaacc
    16921 ggaatctctc gacgatatcc ttggcgatat tctgaaaaaa gagacaacga taacctcgtt
    16981 tttaaatatg ccgacgttat cattgagccg aaccgaatcg agtatgttgc gaatgtggat
    17041 ggcaggtcag ggaaccattc aaatctccga ccaaatgaat atcaaagcca agaccgtttc
    17101 atcgcataaa ggtaatatta aacgtaagat caaaacgcat aataaacagg ttatctacca
    17161 tgtcgtccga ctgacggata atgtgactaa tggtattttt gtcaacatgc gctaacacat
    17221 tctgactggt ggtttcccac cagtcaggct gaataagatt actctgcttt ctcaacaaag
    17281 ataccgtcct gatgccctgc ttcattaaag aaccagatgc cgagcgggta gtcttccagc
    17341 gcaaccaggt acattgtgcc ttcactaaac tcctcaactg ccagtaccac tccaggacga
    17401 cgcggaccgc catccgtttt gactgttacc cgatcattca ccttcatcgt tttcctcctg
    17461 tggctttgtg ccagtgtaga acaatttcgt ttttctggca gcgccaagcg cgcgcgagtg
    17521 ctgattttct cgacggtcta tacttaagag atgccaggcg gacttaacga ctggcggcaa
    17581 caacagagta acggttgcga ggaaagatga tgaaaaccgc aaaagtgtac agcgataccg
    17641 caaaacgtga ggtcagcgtc gatgtcgatg ccctgctggc ggcgatcaat gaaattagcg
    17701 aaagcgaagt tcatcgcagc cagaacgatt ctgaacacgt tagcgtcgat ggacgtgaat
    17761 atcatacatg gcgtgaattg gcggatgcct tcgaactgga tattcatgac ttcagcgtct
    17821 ctgaagtgaa tcgttgaatg cacaataaaa aaatcccgac cctgagggtg tcgggatgaa
    17881 acttgcttaa gcaagaagca cttaaaaaat tcgttacacc aggaaatctg atgtgttcat
    17941 caccttatct gcaatttttt tcgctgacaa gaaaatattc gcgctatgaa tgattaacgc
    18001 tacttatgaa tagaaatgtg acgcgcgtca ccccttggtg gctctggtgt caacgggatc
    18061 gctcccgaaa aaatcatctt tgattatttg tgctgttttg agaatcatcc tggtaagggg
    18121 agtactgcgc gaccatgttt tcaattcaac aaccactact ggtttttagc gatcttgatg
    18181 gcaccctgct ggacagtcat agttatgact ggcaaccggc agccccctgg ctcagccgtt
    18241 tacacgaagc caatattccc gtcattctct gtagcagtaa aactttagcg gaaatgctgt
    18301 acttgcaaaa aatgttgggg ctacaaggct taccgctgat tgcagagaat ggcgcagtga
    18361 tccagcttgc tgaacaatgg caggatatag acggttttcc acgcatcatc tcaggtatta
    18421 gccatggcga aatctgccag gttttaaata cgctacgcga gaaagaacat tttaaattca
    18481 cgacttttga tgatgtcgac gatgcaacca ttgccgaatg gacgggatta agccgtagct
    18541 aggcggcgct gacgcagctg catgaggcgt cggtaacgct aatctggcgc gacagtgacg
    18601 agcgtatggc acaatttatc gctcgtctga acgaactggg cttgcagttt atgcagggtg
    18661 cgcgcttctg gcacgtactg gatgcctctg ccggaaaaga tcaggctgcc aactggatta
    18721 tcgcgaccta tcaacaattg tcaggcagac gcccaaccac acttggcctg ggcgatgggc
    18781 caaacgatgc gcctttactg gaggtaatgg attacgcggt gattgtgaaa gggctaaacc
    18841 gtgaaggggt acatctgcat gatgaggatc cggcccgcgt ctggcgaacg cagcgtgaag
    18901 gaccggaagg ctggcgtgaa gggctggacc attttttctc cgcccgttaa gcgttatcgc
    18961 tcgcgaatac ccgattgcgc ccggcctgtt ttgcgaggta aagccgacgg tcggccaatg
    19021 actggagttg ttcaaaatca taatcaccgg tttcctcgct gctacttacc cccagcgagg
    19081 cactgatgcg tatcgttgta ctcttggcga tcagcatctc tttttcattt aacttcaggc
    19141 gaatacgttc tgcgacctcc gcagcctgcg tcagattcgc gcctggcaga atcacacaaa
    19201 actcctcacc accgacccgc ccggcaacgt cctgcgcacg caaggaactg ctaattaatc
    19261 cggcagcatg agaaagaaca cggtcgcccg cctgatgacc aaagcggtca ttaatcgctt
    19321 taaaatggtc aagatcgacc tgaatgacag aaaaaggatg ttggtgcgtc tgacacaatt
    19381 tagcgagcgg acgggctttt tcgaacagtg cgccacggtt atataaacgc gttaaggtgt
    19441 cgtgccacgc ctgccactgc aacgagcttt gcagaacata catgttgctg accatccggc
    19501 gaatcacata ccaggagagg agtaacatgg tggtaaagag cgcccacagc agggttaatg
    19561 caatgctgat actgccgaaa tcgccgcgca cgccttcgct tagcgtatgg acgcgcgcca
    19621 gcacaccgtc gaaatggtcc agacgttccc agctaacata gcgactgtcc atacgaatgc
    19681 cgccccgcgt gtcatgttcc attgcctgcg ccagcaaggc cagttcacga ggatcaaaaa
    19741 tattccctgt tggatggtca ggattggaag aggtcaaaaa tctcagctta ctgtcataga
    19801 gctgatactc accatcgagg tttttatcga tggcgtttct taaaaattgc tgcatagtac
    19861 gcacgggaat actcatcccc agcacgccat accagtagtt attactgtct accggaacac
    19921 tgacggtaac ctgcggttca gtattgctgg cgtgttccgg ttgcgaagtg aaccagcgta
    19981 ccgcgcggtg acgattttct cgttgcgaat ggccgataaa ccagggttgg gtgacatagc
    20041 cgtaataacg cgttggtaca ttgcgcgtaa acaaggtcgg ctgcgtcgaa acgtaaaatc
    20101 cagcacgcga gacatacatc gcctgttcaa ccatcgacga ggagttgtgc gccagtcgca
    20161 gcaagtaacc aacttccagt gcagcggtaa tttcattgtc gaggctttca ttttcgcgag
    20221 acaggaaatt cccctcgctg actaatgcat ccgatacgcc attgactggc agggtgcgcc
    20281 gtcggttgag ttcgatttgc caggcgtgct cgtcgcgatg ctgttcgaac tcggttacgg
    20341 cattacgcag agaggtgaaa tccagtggcg ccacgagagc ttcgcgcatg ccattacgca
    20401 aaaagatcag tttgtcgacg ttatactgca aatgcttatc gagcgcgttg gctacgtttt
    20461 ccagatgatt acgctagctg gagatatagg catcttccag caccaccact tcacgccagg
    20521 tgagcaaggt ggaaaaaagc aataccacga taaagcagag attaacgaca tgaccgggcc
    20581 ccaggcggcg tgcgagtttt ttcaaccagc tctggttttc catttttgtc tcgtgctgca
    20641 ccctgacgac tcccaacctg cttttttatg attctggcat aacccggcaa tagcgtaccg
    20701 caaaacagcg ataattttat aatggtggca tgataagacg cgactatcat tcattgcagg
    20761 cattgggaca ccaggccgga tgtgatccgg cctggtgtta atgataccct ttcaaacttc
    20821 ttccgcagaa cgactcccct cccccggaac aagatcggtt tcatgaaaag cttcccgctt
    20881 gaccccgtaa ccgtcatacc aacgacactc aaccataccg ctggagtatc cagtgacaat
    20941 catccgcggg ccgccctctt taaccgtaac ttcctcacta accataaagc tcatactcgc
    21001 ctcctttttt cgagtgaaac ttgttcacct tagttgaaga tggcgaattt tgcataatag
    21061 ccagtgcgag tattaatgtg cctgaccgcg cattttcgct actgctttca ctcccagcac
    21121 cacgatgccg ccgatgataa atccaagaat cagatttaaa acagtcggta atatcatcgc
    21181 caccactgca ctttgctgcc cggcgatatg ttcaatagcg tgatgcagcg gcgcaatacc
    21241 atgtaccaca atcccgccgc cgacgaggaa catcgccagc gtgccgacaa tcgataatgc
    21301 tttcatcagc caaggcgcga taatcaataa ccctttacct aatgcctgca tcagcgcgct
    21361 agatttttcc gccagccaat accctaggtc atcaatctta acgataaccc ctaccagacc
    21421 gtagacgccc acggtcacta ccagcgcgat gcctgaaagc accagcacct gattaagcaa
    21481 cggcgcctct gccacaatcc ccagcgtgat ggcgacgatt tccgcagaca aaataaaatc
    21541 ggtacgaatc gcccctttaa ttttgtcctt ttcaaacttc agcggatcct gcgccgccag
    21601 cttctccaga cgctgctggc tctgcgctgg atcttcttta tgtttacgcg cctccagcat
    21661 atgcagcact ttctctaccc cttcaaagca gagaaacgcg ccaccaatca tcaacagtgg
    21721 cgtaatcgcc cacgggataa acgcactgat gatcagcgcc agcggcacca gaatcacttt
    21781 attgatcagc gatcctttcg ccacgcccca gaccacggga agttcccggt tggcccgcac
    21841 gcctgaaact tgttgcgcat tgagcgataa atcatcccct aatacaccgg cggttttctt
    21901 cgccgccagt ttgcccatca cagagatatc gtccagcagt gtggcgatat catcgagcaa
    21961 cgtcagtaaa ctactcccgg ccaaaagcat tccctctcat ttttattgtt gaagggtgta
    22021 agtatgaagc aaaaacggca gctctgatac ttgcttcaca ggtcaacatt tatttaacaa
    22081 tcaagtgcaa ttatatctct cgccagcaga atgattttca cgtttactat gaaccacctt
    22141 tttatttccg ccgtgaggga ttatgcgttt ccgccagttg ttaccgcttt ttggcgcgct
    22201 gtttgcgttg tatatcattt ggggctcaac ctattttgtc attcggattg gcgtgggaag
    22261 ctggcctccg ttaatgatgg cgggcgttcg attccaggca gccggtattt tattgctggc
    22321 atttttgcta ctgcgcggac acaaacttcc cccgctacgt ccgctgctca atgccgcgct
    22381 gattggcctg ttattgctgg ctgtcggtaa tggcatggtg acggttgccg aacatcaaaa
    22441 tgttccttcc ggcatcgccg ccgtagtgat tgcaaccgtg cccctcttta ccctgtgctt
    22501 cagtcgcctg tttggcatta aaacgcgcaa actggaatgg gtgggtattg ccattgggct
    22561 tgccggaatc atcatgctca atagcggtgg aaatttaagc ggcaatccgt ggggcgcgat
    22621 tctgatttta atcggctcga ttagctgggc gtttggctca gtttatggct cgcgcattac
    22681 cttacctgta gggatgatgg cgggtgcgat tgagatgctg gcggcaggcg tggtgttaat
    22741 gatcgcatcg atgattgcgg gtgaaaaact gacggcgctc ccttccctgt caggcttcct
    22801 tgcggtcggc tatctggcgc tgtttggttc gattatcgcc attaacgctt atatgtattt
    22861 aatccgtaat gtcagtccgg ctctcgccac cagctacgcc tacgttaacc cggtggtcgc
    22921 ggtcttgctg ggtacgggac tgggtggcga aacgctgtcg aagattgaat ggctggcgct
    22981 cggcgtaatt gttttcgcgg tggtactggt cacgttggga aaatatctct tcccggccaa
    23041 acccgttgtc gcgccagtta ttcaggatgc atcaagcgag taaatgaatc ccctgcgtgt
    23101 cgatctgcgc gctggcacct tcgccgcaga tccactcttc cagacgctcg gtaagcgccg
    23161 catccgtcag cttctcgcgc ctacgtaacg cacactccca gacaatcagt acgcgccagc
    23221 cgagctcctg caagcgactg atatcgcggc gatcgcgttc aacattttta cctatcttct
    23281 ccagccagaa ctcagttcga gtcgcaggca ctttaaacag atagcagtga tgatgatgcc
    23341 agaagcagcc gtgggtaaat atcacgcagc gatattcatc aacgacaaaa tccgggcgtc
    23401 cgggcagact ggcgtcctga acgcgaaacg caaggccctg cccggttaac aaactggcga
    23461 ggcgcttctc aatcgccgta tcacgcgtgg caatcgcgcg catatttttg ctgcgggtgg
    23521 ccttatcgtg aacgtcgacc atgttgtgcc tcttgctgac gcaacgccac cgcctgtttg
    23581 atttttggct caagcagttt tgccaccgcg gcaaagaccg gcacgaccac cgagttgccg
    23641 aactggcgat aggcctgagt gtccgaaacc ggaatgcgga atttcgcttc tcccggcgct
    23701 tcaaaaccca ttaagcgcgc gcattcacga ggcgttaacc gacgtggacg atgttgctga
    23761 ttcagcggat cgtcaaagtc tttctcaccc gtggccatat cccaaccgcg atcgattaaa
    23821 atttccgcgc catctttgta ataacgagca gacagcgtac gggtgacgct ttgcggattg
    23881 ttcggataaa ccattccata accgaagccg ttaccgcgcg cctgatgttt tttcgcatat
    23941 cgatagaggt acttccacag caccggcgtc aggatatatt tcgcctcaac catcgggtcc
    24001 agcagctgcg ccagcgtcac tcgctgtgca gggaaacatt cgctgatatc acgcagagta
    24061 aaatcggctt tcagattcag atcgcgacga aaacccacca gcacgatgcg ttcacggtgc
    24121 tgcggcagga aatgtttacc atcgatgatt ttcggatcgt ccggcccgtt atcttctgca
    24181 tcggcaacgt catagcccag ttcgtccagc gtctgcatga tgatgcggaa cgtttttccc
    24241 ttatcgtgac tcttcaggtt tttgacgttt tcgagcacaa acatcgccgg acgacgcgcg
    24301 tcgataatgc gcaccacatc aaagaacagc gtgccctggg tatcgcaggc aaaaccgtgc
    24361 gcccgcccga gcgagttctt tttcgatacg ccagccagcg aaaatggctg acaagggaaa
    24421 ccggccagta aaacatcgtg ttcaggaatg tgttgacgaa tatgttccgc cgccgcctca
    24481 tcactcacgc cttcttgatg actgagggtg atgtcgcgga tatcttcatt aaaatgatgc
    24541 gtcgccgggt cgcaataatg gttggcttta taagtgcgta ccgcatgttt gttccattcg
    24601 ctggtaaaca cgcactgtcc accaatcgat tcaaaaccac gacggatgcc gccaattccg
    24661 gcaaatagat cgataaagcg aaacgcataa tgcggatgat gttccggtgg tttgggtaac
    24721 aacgtttgca gatgggcaaa ctctttctca cttaaacggt gccatgccga gtcattcgcc
    24781 agcgcacgtt ttaaaattgc cgcgctccag tgattctcac ccacaccatt aagctgcgcc
    24841 accagcattt taacatcata aatttgcagc agtttctcca gcattgcctg tgcggcattc
    24901 ccggtgctgt atgaatcggt tactgatata ttttcctgca tagatttaac cggccatctg
    24961 agatggccga caggttaaca taattactgc cgtttagggc cacgcgctca gccagcgggt
    25021 gaaaacgcat ctatcacctt gtgatcgata ccttcattct cccccgccag ttcggcaccg
    25081 agcttcgcca tcaaacagcg ccaagctcaa tgcccctgaa acggcaaata cgcgcgccag
    25141 gccaatcgcc cccagggctt ccagccgatc cgcatcctgg acgattttag cttccgttgt
    25201 taagggggcg atttgcgcgc tgaaactgtg agcggcaatg gcatgacaaa cggcctcaat
    25261 tttctccgcc ggaaattgca cgaactcttc acggagcagg cgacgcgtct cttctgccgc
    25321 caggattgaa gaacgctgcc gttgcggatg atttttcgcc aggctcacga tatcgtgaaa
    25381 ataacaggcg gttaaaatca ccagcatatc gacatcgtca tctgccgcca gtttctgtgc
    25441 cgtagcccag acacggcgaa aatgacacag gtcatgcgcc gcgtcctgat gttgatgatg
    25501 gtttttcaac cagttctcga actatgcctg ccagtgttgt aactccatac gcgctccgtt
    25561 gccaaagagt ctcaacctta gcagttttta ttcatagcgt ctgcaacgtg cgggacgagg
    25621 tcgggtgtac catgcaatgc cgcctaacaa ggcaccaacc agggccataa acagaaatcc
    25681 gaccaatgct cctaaccact tccccgcggt agatcctaaa tcactttcaa atcccgacac
    25741 gggggcatcg gggatccggc tcatcaccca gatgtatcgc gccattgccc agacgaaata
    25801 gccacaccat gtatagaaag c
//
LOCUS       DAJTUI010000056        25206 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTUI010000056 DAJTUI010000000
VERSION     DAJTUI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25206
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.056"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..1357)
                     /gene="yeaG"
                     /locus_tag="OO464_002448"
     CDS             complement(<1..1357)
                     /gene="yeaG"
                     /locus_tag="OO464_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6227562.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1793..2539
                     /gene="mipA"
                     /locus_tag="OO464_002449"
     CDS             1793..2539
                     /gene="mipA"
                     /locus_tag="OO464_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6227563.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2629..3390
                     /locus_tag="OO464_002450"
     CDS             2629..3390
                     /locus_tag="OO464_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR6227564.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3441..4325)
                     /locus_tag="OO464_002451"
     CDS             complement(3441..4325)
                     /locus_tag="OO464_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6227565.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4409..5404)
                     /gene="gapA"
                     /locus_tag="OO464_002452"
     CDS             complement(4409..5404)
                     /gene="gapA"
                     /locus_tag="OO464_002452"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6227566.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5746..6159
                     /gene="msrB"
                     /locus_tag="OO464_002453"
     CDS             5746..6159
                     /gene="msrB"
                     /locus_tag="OO464_002453"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6227567.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6201..6473
                     /locus_tag="OO464_002454"
     CDS             6201..6473
                     /locus_tag="OO464_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6227568.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6843..7919
                     /locus_tag="OO464_002455"
     CDS             6843..7919
                     /locus_tag="OO464_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6227569.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7946..9325
                     /locus_tag="OO464_002456"
     CDS             7946..9325
                     /locus_tag="OO464_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6227570.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9342..10387
                     /locus_tag="OO464_002457"
                     /pseudo
     CDS             9342..10387
                     /locus_tag="OO464_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10408..11244
                     /locus_tag="OO464_002458"
     CDS             10408..11244
                     /locus_tag="OO464_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR6227571.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11249..12196
                     /locus_tag="OO464_002459"
     CDS             11249..12196
                     /locus_tag="OO464_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR6227572.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12206..13186
                     /gene="ydjG"
                     /locus_tag="OO464_002460"
     CDS             12206..13186
                     /gene="ydjG"
                     /locus_tag="OO464_002460"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR6227573.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13323..14081
                     /locus_tag="OO464_002461"
     CDS             13323..14081
                     /locus_tag="OO464_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6227574.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14199..15557
                     /locus_tag="OO464_002462"
     CDS             14199..15557
                     /locus_tag="OO464_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6227575.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15650..16291)
                     /gene="pncA"
                     /locus_tag="OO464_002463"
     CDS             complement(15650..16291)
                     /gene="pncA"
                     /locus_tag="OO464_002463"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6227576.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16302..17318)
                     /gene="ansA"
                     /locus_tag="OO464_002464"
     CDS             complement(16302..17318)
                     /gene="ansA"
                     /locus_tag="OO464_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6227577.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17485..19341)
                     /gene="sppA"
                     /locus_tag="OO464_002465"
     CDS             complement(17485..19341)
                     /gene="sppA"
                     /locus_tag="OO464_002465"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6227578.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19502..20053
                     /locus_tag="OO464_002466"
     CDS             19502..20053
                     /locus_tag="OO464_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6227579.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20170..21213
                     /gene="selD"
                     /locus_tag="OO464_002467"
     CDS             20170..21213
                     /gene="selD"
                     /locus_tag="OO464_002467"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6227580.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21218..23179
                     /gene="topB"
                     /locus_tag="OO464_002468"
     CDS             21218..23179
                     /gene="topB"
                     /locus_tag="OO464_002468"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6227581.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23306..24346
                     /locus_tag="OO464_002469"
     CDS             23306..24346
                     /locus_tag="OO464_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6227582.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24463..25191)
                     /locus_tag="OO464_002470"
     CDS             complement(24463..25191)
                     /locus_tag="OO464_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005107564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR6227583.1"
                     /translation="MRPEATGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIE
                     KAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEG
                     QQPWSVPVDIALPCATQNELDVDAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVL
                     FAPGKAANAGGVATSGLEMAQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQT
                     NYVQGANIAGFVKVADAMLAQGVI"
BASE COUNT         6410 a         5841 c         6511 g         6444 t
ORIGIN      
        1 gctgttccag gacgtagaac agatggaccg ggtttgccgc cacttctaca tgatcgaagt
       61 tgaacacgcg ggagaggatc ttaaacgcaa aacgcgtcga cagaccgttc atcccttcat
      121 cgacaccggc gtagtcacga tattcctgat acgacttggc tttcggatcg gtgtctttca
      181 gactttcgcc atcataaacc cgcatctttg aataaatgct ggagttttct ggctctttca
      241 ggcgcgaaag aatggaaaaa cgtgacagtg tttcgagcgt gccaggggcg catggggcgt
      301 gagtcaattc actgtgatta agcaatttct cgtagatttt gatctcttcg gaaatgcgca
      361 agcaatacgg caccttcacg atgtaaacac ggtcgaggaa ggcttcgttg tttttgttat
      421 tacggaaagt gacccattcg gacttgttcg agtgggcgag aataatcccg ttgaacggca
      481 gggcggagat accttccgtc ccgttgtagt taccttcctg agtggcggtt aacaagggat
      541 gcagcacttt tatcggtgct ttaaacatct caacgaattc catgatcccc tgattggcgc
      601 ggcacagcgc accggaatag ccgtaggcgt ccgggtcatt ctgcgcgtag tgttcgagtt
      661 tacgaatatc gactttccca accagcgcgg agatgtcctg gttgttctca tcaccgggtt
      721 ccgttttggc gatagcaatt tgttgcagaa ttgacggcca gaccttcact acccggaact
      781 tagtgatatc gccaccaaat tcatgcaggc gttttgccgc ccatggcgac atgatggtgc
      841 cgagataacg gcgaggaatg ccatactctt tttccagaat ctgagcatct tcctgcggat
      901 tgaaaagaca gaacggatga tcgttgaccg ggctacgctc accgttggcg ctcaatacat
      961 aaatcggtac gagctgcatt aatgatttca gtcgctcagc aagcgatgat ttacccccac
     1021 ccacaggccc cagcagatac aggatttgtt tcttctcttc cagcccctga gccgcgtgtt
     1081 tcagataaga gacaatctgt tcaatcgcgt cttccatgcc gtaaaactct tcaaatgccg
     1141 gataacgtgc aatgacccgg ttagaaaaga gtcgagaaag tctggggtcc tgggctgtat
     1201 cgaccatgac aggctcaccg atagccatca atagccgctc agccgcgttg gcataagcac
     1261 tgcgatcttg ccgacaagtg gtaagaaact cctgcagtgt gaactcttcg tccttggcag
     1321 cttcatatcg ctggcgatag tgatcgaata tattcatggt atgccgtcct ttcgtttttt
     1381 agcacaggtt aagagccgtt catatgaata gtagaggctc ccggaagagg taaactgaac
     1441 gacgtctctt gctaaaacac agcaactctc atgccatttt atcgacgatg agaacaacgt
     1501 gcccggtgat acaccttcaa aattaagcgt agatggcaaa tggaaaactt gcctgcacgg
     1561 ctttcttaat ttcaatggca tatcaacaac tcatccataa attttgcata attaatgtaa
     1621 agaccaggct cgccagtaac gctaaatcca tttggctgta agcgcggtgt catccgcggc
     1681 aggaaaatta aacagttact ttaaaaaatg aaaacgtaaa aaggttgggt ttcgatgtat
     1741 tgacgggtaa actttgtcgc ccgctaaaca tttgtttaag gaatgattaa ttgtgaccaa
     1801 actcaaactt ctggcacttg gagtgcttat cgcaacgtct gcaggcgtag cgcacgctga
     1861 aggtaaattt tccctgggcg caggcgtagg tgtcgttgag cacccatata aagattacga
     1921 taccgatgtt tacccagtac cggtaatcaa ctatgaaggc gataacttct ggttccgtgg
     1981 cttaggtggt ggttactacc tgtggaatga cgcaacggat aaactttcaa ttaccgctta
     2041 ctggtcgccg ctttacttca aagcgaaaga cagtggcgat caccaaatgc gtcacctgga
     2101 tgaccgtaag agcaccatga tggctggtct gtcttatgct cactttaccc agtacggtta
     2161 cctgcgtacc accctggctg gcgataccct ggataacagc aacggcattg tctgggatat
     2221 ggcctggttg tatcgttaca ccaacggtgg cctgaccgtg actccgggta ttggtgtgca
     2281 gtggaacagc gaaaaccaga acgaatacta ttatggcgta tcgcgcaaag agtccgctcg
     2341 cagcggtctg cgtggctata acccgaatga cagctggagc ccttaccttg agctgagcgc
     2401 cagctacaac ttcctcggcg actggagtgt ttacggtacc gcgcgctaca cccgtctgtc
     2461 tgatgaagtt actgacagcc cgatggtgga taaatcctgg actggcctga tttctaccgg
     2521 gatcacctac aaattctgat aatgcatctt tccagggcat tttgtgcata aaaacagggc
     2581 gtcacgccct gttttgcatt acggtggtgc aatacctggg aggaaacaat gcaacaaaaa
     2641 atgattcaat ttagtggcga tgtctcactg ccagccatag ggcagggaac atggtatatg
     2701 ggcgaagatg ccagtcagcg caaaacagaa gttgctgcac tacgcgcagg cattgaactc
     2761 ggtttaaccc tcattgatac cgccgaaatg tatgccgatg gcggtgccga aaaggtggtt
     2821 ggggaagcat taaccggtct gcgagagaag gtctttctcg tctctaaagt ctatccgtgg
     2881 aatgctggcg ggcaaaaagc gataaatgca tgcgaagcca gtttacgccg tctcaatact
     2941 gactatctcg atctctactt attacactgg tctggcagtt tcgcttttga agagactgtt
     3001 gctgcgatgg aaaaattgat cgcccaggga aaaatccgtc gctggggcgt ttctaacctt
     3061 ggttatgccg atatgcagga actctggcag ctgccggggg gaaatcagtg tgccactaat
     3121 caggtgcttt accatctcgg ttcacgaggg attgagtacg atctactccc ctggtgccag
     3181 caacagcaga tgccggtgat ggcttacagt ccattagccc aggccgggcg gttgcgcaat
     3241 ggactgttaa aaaacgcggt agtcaacgaa attgcacatg tccaacaaca tgcagctgcg
     3301 cttgaggtcg aactttcgtc agcggaatta gctatgctgg ataaggcata tccggcacca
     3361 aaaggaaaaa ctgcgctgga tatggtgtga tgttgatacg tgccggatgc atcgcacccg
     3421 gcacacaaca ttaacgcaaa ttaacgtttg gcaacgcgaa tggattgcgc cagatgtgca
     3481 ggtttctctt tggtgacttt ttgcgtttct gaagcgtaag ccgtttctac acaaacaaag
     3541 gtcttgtagc catcgtccgg catgtcgccc atgctaactg aaagcgccgg tcccgggttc
     3601 cagccgacaa cgttcagatg atgctggtgg cctacggcga taatacgatt cagcgcttca
     3661 tcattaatca cgctgcaatc ttgcggattc agatacacgc gatcggtacg gtcagggaag
     3721 gtctgaatac cgtcggtcag tacatcttct ttcgcgtcat tcactttatc aatgaagcga
     3781 tcgcccagcc cactgacgct taccttagcg atatcaccca cgttaaagta ggtatgcagg
     3841 gcagaggtgg tttcaaattc gccatgtgat tcaagatcga tttcacaggt tttacccacg
     3901 cggaaatgcg ctaacagcgt aaagtcgtgc ggccagaatt ttttcgtctc ttcgctttgt
     3961 gtcaattcaa aagtcagcgc tacgccatca gcatcttcat gatgtgattt cagcgtccac
     4021 ggcaggttgc gggcaaaacc gtgcgcaggc agaccttgtt gtgccgccgg accaaaccac
     4081 ggccagcaaa ccggtacgcc accgcgaata gcgacgccat ttttgaacgg tgtgttgttg
     4141 ctcaaccaca gaacttcttc ttcacccgca ggtttccacg agagaaggtg tgcgccctgt
     4201 aatgcaaaag aggcttttac ctggggatga tcgaccacaa tgaggtccag ttcatccagt
     4261 ttacgacggg agaggacagg ggagatttgt tcgatgaccg gaagggcaaa aattttctta
     4321 atcatgacgc agtcctttaa cttcatttta tcaggtaaaa aaaagagcga ccgaagtcgc
     4381 tctttttaga tcacagtgtc atctcaactt atttggagat gtgagcgatc aggtccagaa
     4441 ctttgttgga gtaaccggtt tcgttgtcgt accaggatac cagtttcacg aagttgtcgt
     4501 tcagagcgat accagcttta gcatcgaaca cggaagtgca aacttcgccg ttgaaatcgg
     4561 tagatactac gtcatcttcg gtgtagccca gaacgccttt catttcgcct tcagcagcag
     4621 ctttaacggc agctttgatc tgctcgtaag ttgcagcttt ttccagacga acggtcaggt
     4681 caactacgga tacgttcggg gtcggaacgc ggaacgccat accagtcagt ttgccattca
     4741 gttctggcag tactttacct acagctttag cagcaccggt agaggacggg atgatgttct
     4801 gggaagcgcc gcggccgccg cgccagtctt tgtgagacgg gccatcaacg gttttctgag
     4861 tagcggtagt agcgtgaacg gtggtcatca gaccttcgat gatgccgaag ttatcgttga
     4921 taactttagc cagcggagcc aggcagttgg tggtgcagga agcgttggaa acgatgtcct
     4981 ggccagcata tttgtcgaag ttagcgcctt taacgaacat cggagtgttg tctttagacg
     5041 gaccagtcat aaccactttc ttcgcaccag cggtgatgtg tttacgagca gtttcgtcag
     5101 tcaggaacag accagttgct tcagcgacaa cgtcaacacc cacttcgtcc catttcaggt
     5161 tagccggatc acgttcagcg gtaacacgga tttttttacc gttaacgatc agatgaccgt
     5221 ctttcacttc aacggtaccg tcgaaacggc cgtgagtgga gtcatatttc agcatgtatg
     5281 ccatgtaatc agcgtctaac aggtcgttga ttgcaacgat ctcgatgtca gaacgtttct
     5341 gagcagcacg gaaaacaatg cgaccgatac ggccaaaacc gttgatacct actttgatag
     5401 tcatatattc caccagctat ttgttagtga ataaaaggtt gcctgtaaaa ttacaaaaac
     5461 cttacgcagc gtcaagcgga atcgtgtcaa tcattgcgac aaatcaatcc tgtgcctaag
     5521 cattacgcga ctgactcgcc tcacttttcc tttgggcttg agaccacatg gggtcggcgc
     5581 cccgaatttt aaagggcaat tacgataaaa atgtgatttt catcacgatt taggtgcagc
     5641 catttcgtcg agatcaagtt taggacaaaa ttttgccagt tggtgttaat gttttgttag
     5701 aatcagtcac gtaatgtgag cacgattaaa gtgagatgtg agtaaatggc gaataaacct
     5761 tcggcagaag aactgaaaaa aaatttgtcc gagatgcagt tttacgtgac gcagaatcat
     5821 gggacagaac cgccatttac gggtcgttta ctgcataaca agcgtgacgg cgtatatcac
     5881 tgtttgatct gcgatgcccc gctgtttcat tccgaaacca agtatgattc cggctgtggc
     5941 tggcccagtt tctacgaacc ggtgagtgaa gaatccattc gttatatcaa agacttgtca
     6001 catggaatgc agcgcataga aattcgttgc ggtaactgtg atgcccatct ggggcatgtc
     6061 tttcccgacg ggccgcaacc aacaggcgaa cgttattgtg ttaactctgc ttctttacgc
     6121 ttcactgatg gcgaaaacgg cgaagaaatc aacggttgaa aaaacgattc agcaacttat
     6181 tccacaggag cggaaaaatt atgaatcttg atgacattat caacagcatg acgcctgagg
     6241 tataccagcg tttgtcgacc gccgttgaac tggggaaatg gcctgatggc gttgcgttga
     6301 ccgaggaaca aaaagagaat tgcctgcaac tggtgatgct gtggcaagcc cgccataata
     6361 ccgaagcaca gcatatgaca attgacacaa atggtcagat ggtcatgaag agcaaacagc
     6421 agttaaaaga agattttggt atctcagcaa agcctattgc catgtttaag taagtggttc
     6481 atcccctttt tgccgcggct atgaagggga tagccaacaa tgaccccacg actcgtctca
     6541 attatctcga cccgacctgg ctaagtccca gtccgatctc ttgcagtcgc gttcacgcct
     6601 gaatgacttc tcctgaactt aagtctcaga cctatttggc cggtaatccc tctcgaattt
     6661 gctctttttg cgtcccgcct tccaccaacc tgtaacagac ccagccattt tctgcgtaat
     6721 tgtgatttga gttgtaaaat gcaggtcatt atctgtgttg tgcagattta atattgacct
     6781 caaaaatcat gtgggtaagt atgatggcca tctaagcaaa acaaaacaca gaggatgacg
     6841 caatgaaagc actggctcgg tttggcaagg cctttggcgg ctacaagatg attcatgtcc
     6901 cacaacccat ttgtggcccg gaagacgtag tgactgaaat taaagccgcg gcaatctgcg
     6961 gcgcagacat gaagcactac aatgtcgata gcggttctga tgagtttaac tctatccgcg
     7021 gccatgagtt cgcaggttgt attgcgcagg ttggtgaaaa agtcaaagac tggaaagtgg
     7081 ggcaacgcgt cgtgtcggat aacagcggtc acgtttgcgg cgtttgtccg gcctgtgaac
     7141 agggtgattt tctgtgttgt acagaaaaag taaaccttgg tctggataac aatacctggg
     7201 gcggtggttt ttccaaatat tgtctggttc ctggtgaaat tctcaaaatt catcgtcatg
     7261 cgttgtggga aatccctgat ggtgttgatt atgaggacgc agccgtactt gaccctatct
     7321 gtaatgccta taaatccatc gcgcagcaat cgaaattcct tcctggtcag gatgttgtcg
     7381 tcattggcac tggcccactc gggctgtttt ccgtacaaat ggcgcggatt atgggggcgg
     7441 taaatatcgt cgtcgttggt ctgcaagaag atgtggcggt ccgcttcccg gttgcaaaag
     7501 aactgggggc gacggcagta gtaaatggtt ctaccgaaga tgtggtggcg cgctgccagc
     7561 aaatttgtgg caaagataat ctgggactgg tgattgaatg ctccggtgcc aatatcgcac
     7621 tgaaacaagc catcgaaatg ctccgcccga atggggaagt ggtacgcgtt ggaatgggct
     7681 tcaaacctct tgatttctcg attaatgaca ttaccgcctg gaataaaagc atcattgggc
     7741 atatggccta tgactccacc tcatggcgta acgctatccg gctattagcc agcggcgcta
     7801 tcaaagtcaa accgatgatc acgcatcgta tcggcctgtc gcaatggcgc gaagggtttg
     7861 atgcgatggt cgataaaacc gcaatcaaag tgatcatgac ttacgacttt gatgaataac
     7921 tactctcctg aaggaacgtt atcgcatgga acagataaca aaaccgcatt gtggtgcacg
     7981 gctggatcgc ttaccggatt gccgctggca ttcgtcaatg tttgccatcg tcgcgtttgg
     8041 cttgcttgtc tgctggagta atgccgttgg tggcttgatc ctcgcgcagc tgaaagcgtt
     8101 gggctggaca gataattcca ctacagccac attctcagca atcacgaccg ccggaatgtt
     8161 tctcggtgcg ctggtgggcg gcatcattgg cgacaaaacc ggtcgcagaa atgcgttcat
     8221 cctctatgag gccattcata ttgcctcgat ggttgtcggt gctttctcac cgaatatgga
     8281 ttttcttatt gcctgccgtt ttgtgatgga cgttgggctg ggagctttac tggttacact
     8341 gtttgctggt ttcaccgaat atatgcccgg tagaaatcgc ggaacgtggt ccagtcgggt
     8401 ttcctttatt ggcaactggt catatccgct ctgctcactg attgcgatgg gactcacgcc
     8461 gctgattagt gcagagtgga actggcgggt acaactgctt attcctgcaa tattgtcgct
     8521 tatcgctacg gcgctggcct ggcgctactt tcctgaatcc ccgcgctggc tggaatcgcg
     8581 cggacggtat caggaagccg agaaagtcat gcggagtata gaagaaggcg tcatacgcca
     8641 gacgggtaaa cctttgccgc ctgtggttat tgctgatgac ggtaaagcgc cacaagcggt
     8701 gccgtattca gccttactga caggagtatt actgaaacgc gtgatattag gctcttgtgt
     8761 gctgattacc atgaacgttg tgcagtacac actaattaac tggttgccaa caatattcat
     8821 gacccagggg attaatttaa aagactcgat tgttttaaat accatgagta tgtttggcgc
     8881 gccatttggt atttttattg ccatgctggt gatggataaa attccgcgta aaacaatggg
     8941 tgtggggcta ttaatcctga ttgcggtgct cggatatatc tattcactgc aaaccagtat
     9001 gttgctcata acgctgattg gtttcttcct gattactttc gtctatatgt acgtttgcta
     9061 tgcctcggca gtgtatgtcc ctgaaatctg gccgacagag gccaaactcc gtggctccgg
     9121 tctggcgaat gcggtagggc gaatcagtgg tattgccgca ccttatgccg ttgcagtgct
     9181 gctcagtagt tatggtgtaa cgggagtctt tattcttctg ggggctgttt caaatattgt
     9241 cgcaattgct atcgccacca ttggaattga aaccaaaggt gtctccgttg aaagtttaag
     9301 tattgatgca gtagccaata aataatacgg gtggattcaa aatgaaaaat tcaaaagcaa
     9361 tattgcaggt gccgggcaca atgaaaatta tttcagcaga aataccagtg cctaaagaag
     9421 atgaagtttt gattaaagta gaatatgtcg gtatttgtgg ttcagatgta catggttttg
     9481 aatcaggccc gtttattccg cctaaagacc caaatcaaga aattggcctg ggtcatgaat
     9541 gcgccgggac ggttgtggct gtgggaagcc gcgtgcgcaa atttaaaccg ggggatcggg
     9601 taaatatcga acctggcgtt ccttgcggtc actgtcgtta ctgtctggaa ggcaaatata
     9661 acatctgccc ggacgttgat tttatggcga cacaacccaa ctaccgcggc gcattaacgc
     9721 actatctgtg tcatccggag agctttactt acaaactgcc cgacaatatg gacacgatgg
     9781 aaggggcgct ggtggagcct gccgcagtcg ggatgcatgc cgcgatgctg gcagatgtta
     9841 aaccgggtaa gaagataatt attctgggag caggttgtat tggtttgatg acgttgcaag
     9901 cgtgcaaatg cctgggcgca acggaaattg ccgtcgttga tgtactggaa aaacgtctgg
     9961 caatagggga acagcctggt gcgacagtgg ttattaatgg cgcaaaagag gacactattg
    10021 cacgctgtca gcaatttacc gaagacatgg gcgcagatat tgttttcgaa acagcgggtt
    10081 ctgcggtcac cgttaaacag gcacctttaa tctggtaatg cgcggcggta aaattatgat
    10141 tgttggtact gtacccggcg attcggcaat caatttcctc aaaatcaatc gcgaagtcac
    10201 tatccagacg gtattccgct atgccaatcg ttatccggtc acgattgaag ctatttcttc
    10261 agggcgattc gatgtgaaat cgatggtgac gcatatttac gattatcggg atgtacaaca
    10321 ggcatttgaa gagtcagtta acaacaaacg cgacattatt aaaggcgtta ttaaaattag
    10381 cgattaatta atacgggaga acagaacatg ctcgcagata tcagatattg ggaaaacgat
    10441 gccacaaata aatattatgc aattgcccat tttaacgtat ggaatgcaga aatgttgatg
    10501 ggcgttaaag acgcagccga agaagcgaaa tccccggtta ttatttcttt tagtacaggt
    10561 tttgtcggta acacctcatt tgaagatttc tctcacatga tggtatcaat ggcacaaaaa
    10621 gcaacggtgc cggtaataac tcattgggat catggtcgga gtattgagat tattcataac
    10681 gcctggactc atggtatgaa ttcattaatg cgtgatgctt ctgcatttga tttcgaagaa
    10741 aatattcgtt taaccaaaga ggctgttgat ttctttcatc cgctgggtat tccggtagag
    10801 gcggaattag ggcatgtcgg taatgaaacc gtttatgagg aagcgctggc gggctatcac
    10861 tataccgatc ctgaccaggc ggctgaattt gttgaacgta cgggctgtga ctcattggct
    10921 gtcgccatcg gcaaccagca tggggtttat acgtcagagc cgcaattgaa ctttgaggtc
    10981 gtcaaacgcg tacgcgatgc tgtttctgtt ccgctggttt tgcatggcgc atcggggatc
    11041 agtgatgccg acattaaaac tgcaatttcg ttgggtattg cgaaaatcaa cattcatacg
    11101 gagctctgtc aggccgcaat ggtagcagtg aaagaaaacc aggatcagcc tttcctgcat
    11161 ttagaacgtg aagtacgtaa agctgtaaaa gaacgggcat tggaaaaaat tgaactgttc
    11221 ggttctgatg gcaaagcgga ataatgatat ggataatctc gacgttattt gtataggtgc
    11281 cgctattgtt gatattccat tacaaccggt cagtaaaaat atctttgatg tagattctta
    11341 tcctcttgaa agaatcgcaa tgagcaccgg gggcgatgca ataaatgaag caacaattat
    11401 ttctcgtctg gggcatcgta ccgcattaat gagccgcatt ggtaaagatg cctcagggca
    11461 atttattctc gaccattgtc gcaaagagaa tattgatatt caaagcctga aacaggacgt
    11521 cagcatagat acatctatta acgttggact ggtgacggag gatggcgagc ggacgtttgt
    11581 cacgaaccgt aacggcagtt tgtggaaatt aaatattgac gatgttgatt ttgcgcggtt
    11641 tcctcaggcg aaattattat cactggccag tattttcaac agtcctctat tggatggtaa
    11701 agcgctaaca gaaattttta cgcaagcgaa agcccggcag atgatcattt gtgccgatat
    11761 gatcaaaccg cggttgaatg aaacgctgga tgatatttgc gaagcattga gttatgtcga
    11821 ttatctgttt ccaaattttg ccgaggaaaa attactcaca gggaaagaga cactggatga
    11881 aattgctgac tgctttcttg cgtgcggcgt aaaaacggtg gtgattaaaa cgggtaaaga
    11941 cggctgcttt atcaagcgtg gtgacatgac gatgaaggtg ccggcggtcg caggaataac
    12001 cgccatcgac accattggcg cgggcgataa ctttgcttca ggttttattg cggcactgtt
    12061 agaaggcaaa aatctgcgtg aatgcgcacg ctttgccaat gcaacggcgg ctatctcggt
    12121 tctaagcgtc ggtgccacca ccggcgtaaa aaacagaaag ctggtcgaac aattgctgga
    12181 agaatacgaa ggataatgaa ggcaaatgaa aaagatacct ttaggcacga cggatataac
    12241 gctttcgcga atggggttgg ggacatgggc cattggcggc ggtcctgcat ggaatggcga
    12301 tctcgatcgg caaatatgta ttgatacgat tcttgaagcc catcgttgtg gcattaatct
    12361 gattgatact gcgctaggat ataactttgg caatagtgaa gttatcgtcg gtcaggcgtt
    12421 aaaaaaactg ccccgtgaac aggttgtagt agaaaccaaa tgcggcattg tctgggaacg
    12481 aaaaggaagt ttattcaaca aagttggcga tcggcagttg tataaaaacc tttccccgga
    12541 atctatccgc gaagaggtag aagccagctt gcaacgtctg ggtattgatt acatcgatat
    12601 ctatatgacg cactggcagt cggtgccgcc attttttacg ccgatagctg aaactgtcgc
    12661 agtgcttaat gagttaaagg ctgaagggaa aattcgcgct ataggcgctg ctaacgtcga
    12721 tgctgaccat atccgcgagt atctgcaata tggtgaactg gatattattc aggcgaaata
    12781 cagtattctc gaccgggcaa tggaaaacga actgctgccg ctatgtcgtg ataatggcat
    12841 tgtggttcag gtttattccc cgctagagca gggattgttg accggcacta tcactcgtga
    12901 ttacgttccg ggcggcgctc gggcaaataa agtctggttc cagcgtgaaa acatgctgaa
    12961 agtgattgat atgcttgaac agtggcagcc actttgtgct cgttatcagt gcacaattcc
    13021 cactctggca ctggcgtgga tattaaaaca gagtgattta atctccattc ttagtggggc
    13081 tactgcaccg gaacaggtac gcgaaaatgt cgcggcactg aatatcaact tatcggatgc
    13141 agacgcaaca ttgatgaggg aaatggcaga ggccctggag cgttaaatac tcaacacgat
    13201 tcacctctgt ttctgtgaaa actgtgatga aaatctgtga ttcaaatagg ttattttgaa
    13261 agtaaacatc ggttatgcga taatcgcgct aatgtgatgt aaaaagatac aggggtgtaa
    13321 tcgtggcggc aaaagacagg attcaggcaa ttaagcaaat ggttgccaac gataaaaagg
    13381 tgacagtctc aaatttgagt gggatttttc aggtaaccga agaaaccatt cgccgcgatc
    13441 ttgagaagct ggaagatgaa ggctttttga ccagaaccta tggtggtgct gttttaaata
    13501 cagcggtgtt gacggagaat atccattttt ataagcgcgc ttcatcgttt tatgaagaga
    13561 agctgcttat tgcacgcaag gcactaccct ttatcgacaa taaaaccacc atggcagccg
    13621 attccagtag taccgtaatg gaattgctca aattgttaca ggaccgtagt gacctgacgt
    13681 tgctaaccaa ctcggcggaa gcgattcatg ttctggctca gtcagaaatt aaagtcgttt
    13741 caacgggtgg ggaactaaac aaaaatacac tttccctgca aggaagaatt actaaagaga
    13801 tcatcagccg ctaccatgtc gatatcatgg taatgagttg taaaggtctt gatattaaca
    13861 gcggcgcgct ggactctaat gaagcggaag ctgaaatcaa aaagacaatg atccgtcagg
    13921 cgacagaagt tgcgttattg gttgatcact ctaagtttga tcgtaaagct tttgtccagt
    13981 tagctgactt tagtcatatt aattatataa taactgataa atcaccgggt gcagagtgga
    14041 tagcattttg caaagacaat aatatccagc tggtgtggta atttaagcac acgttgtcaa
    14101 tattatttgt tcctgcgact ggaaataaaa tgatgataaa gatgcggcaa gtgaatttgc
    14161 cgcaattgct accttttata gcaaatccga ggcacccaat ggaacaatat gatcaaattg
    14221 gcgcaagact ggaccgcttg cctttggccc ggtttcatta tcgtatattt ggtataataa
    14281 gctttagtct gttattaacg gggtttttga gttactcagg taatgtcgtc ttagcaaagc
    14341 tggtaagcaa tggatggtca aataatttcc tctatgccgc ctttacctcg gcattaatgt
    14401 ttggttattt catcggctca cttactggtg ggtttattgg tgactacttt gggcggcgca
    14461 gggcgtttcg cataaatctt ctcatcgtcg gtattgctgc aacaggggcc gcttttgtcc
    14521 ctgatatgta ctggctcatt ttctttcgct tcctgatggg aacaggaatg ggggcgctga
    14581 ttatggttgg ctatgcctca tttacggagt ttatccccgc gacggtgcgt ggaaaatggt
    14641 ccgcgcggct ctcatttgtt ggtaactggt cgcccatgct gtctgcggcg ataggcgtgg
    14701 tggttatcgc tttttttagt tggcgaataa tgtttcttct gggtggtatt ggcatactgt
    14761 tagcctggtt tctctcaggt aaatacttta tcgagtcgcc acgatggctg gaagggaaag
    14821 ggcaaatcgc cggtgcagaa agccaacttc gtgaagtaga gcagcaaatt gaaagggaga
    14881 agagtattcg tttaccccag cttactttga accagagcaa cagcaaggtt aaggtaatca
    14941 agggtacctt caggctcctg tttaaagggg aaatgttacg acgtacatta gttgcgataa
    15001 ctgttttaat tgcaatgaac atttcgcttt ataccattac cgtatggata ccgaccatat
    15061 ttgttaactc cggcattgat gtcgataaat caatattaat gaccgctgtt attatgattg
    15121 gtgctccggt aggaatattt attgcggcat taattattga tcattttcct cgtcgattat
    15181 ttggctccgc cttacttatt attattgccg tgttaggcta tatctattca agtcagacta
    15241 cagagtgggc gattttaatc tatggtctgg tgatgatctt ctttttatac atgtatgttt
    15301 gcttcgcgtc ggcggtttat atcccggagc tttggccaac gcatttacgc ctgcgcggtt
    15361 cgggtttcgt taatgccgtc ggacggatcg tcgcagtttt cacgccctat ggcgttgcgg
    15421 cattattaac acattatggg tcgatcacgg tgtttatggt gcttggtgtc atgttagtgc
    15481 tctgtgcgct ggttctctcc atttttggca tcgaaacgcg gaaggtgtcg ctggaagaga
    15541 tttctgaggt gaattaaatc tttcgttgat ggcatcaatt gccagatgta ccactgacgt
    15601 gttttatttg gcctacaatg ccagtacaga attgtaggcc agcgtaaaat tacccctgcg
    15661 tctcttccca gtctgccagc gtatatagcg ttgccccagc tgctgacatc tccataaacg
    15721 cgtgtgcact gtcctggggc tggatattca cgccacgaca gccatcggta atcacgttta
    15781 ccttataacc taactgtaac gcgtccagca cggtaaactt cacgcaatag tcagtagcca
    15841 ggcccataac gatcaattca tcgatttcat gatcgcgcaa ccagtcatca agcgcggttt
    15901 tctgccgacg tccgttatca aaaaaggcac tgtaactgtc aactaaagga ttttcgcctt
    15961 tatggaacac cgctgcgatc tctttttgat tcagtaacgg atgtaattgt gcgccttcac
    16021 tgttctgcac acagtgttca ggccagaaag tttgccgcag gccgccgagt tggcctggcg
    16081 tataaggctc tacgccgtgc tggctggcaa agctgccgtg attcgccgga tgccagtcct
    16141 ggctggcgat aaccgcttct ccgcgcgact ggcaccagtc aataaggcgg ttagcaacct
    16201 ccaccgtact gtcaccttcc ggtacggcga gcgcgccacc agcacagaaa tcattttgta
    16261 aatcgaccag taacagggcg cgatggggca ttgcagtctc cttaatcatc cggcgtcagt
    16321 tcgccgcgca ggttttggct catggccttg cgaatggttt cagtatccag ttcctggctc
    16381 agcaggtaat gcagtttggt tagcgtggct tctacagtca tatctgcacc gtcaataacg
    16441 ccggcatggg cgagggcgtt accggtggca taaccactca tattcacttt accggacata
    16501 cattgtgtca ggttgaccac cacaataccg cgatcgctgg cttcttgtaa ttcctgcagg
    16561 aaggctttgt tttgtggcgc attacccacg ccataggagc gcagaatcaa tgctttcacc
    16621 ggttggcgca gaaaattgcg cacgacgtca gcagaaatcc ctggataaat cgtcaccacg
    16681 ccaattggtt gtggcgtgat tggatgaacg attaattccc cttcaccgtg cggggcgggt
    16741 ggcgtattca aacgacgaat atggatacca gcttccagta acggaggaag gtttggagag
    16801 gcaaacgcat caaaaccatc ggcatgggct ttggtagtgc ggttgccgcg atacaatcgg
    16861 ttattgaaaa agagcgttac ttcgttgatc ggataattcg gcgcaacgta caacgcattc
    16921 agcagattaa tttgtccgtc agagcgtaac tcagccagcg ggatttgtga ccctgtcaca
    16981 atgaccggtt taccgagatt ctcgagcatg aacgacagcg cagaggcggt atacgccatc
    17041 gtgtcggtac cgtgtagaat gacaaaacca tcatagtcgt catagtgcgc tttaatatct
    17101 tcagcaatat gctgccagtc ttccggcgtc atatcagaag aatccatcaa cggcgtatat
    17161 tcatgaatgg tgaaatctgg catctccggg cgatggaatt ccggcatcag cgccagttgg
    17221 cgttgtagat gacctgacac cggaatataa ccctgctcgg aacgctgcat cccgatggtc
    17281 ccgcccgtgt aggcgacgta aattgatttc ttttgcatga tattgatatg aatgatatcg
    17341 aaagagcaaa agtatatacg tgaggcagga ggagcgcacc tgatactatg cggataagga
    17401 aatatggggc gagacagcgg gaagaggatt cgtcgaggta aaaaataatg tggccgacga
    17461 tctgtcggcc actcagtaca agacttaacg cacgttggcg caggtcaggc aaaacgcata
    17521 acggttttgt gggtcgttaa acgcggccag cttgtcgctt tcacttttaa cggtagaggc
    17581 taccgaggca agcggtgcag gtaacatggc ctggaacgca tctggcaaca ttgcccggac
    17641 agaaccagac atgttgtcca tcactttgtc gaagaaggtc ggttcatcaa cgtagtattc
    17701 cagatgccac tgtttcactt ttgcgagctc tgctgctttg gcaaccgcat catcgaaatc
    17761 cccgagacta tcgaccagcc cgttagcttt tgcatcctga ccggtccaga cgtggccctg
    17821 ggcgatttta tcaatttgct ccggcgtcga atgacgcgca tcagcaacca gcgtgataaa
    17881 gcgtttatag ccattctcaa tgcttaattg catcatctgc tgcgcttccg gcggcagtgc
    17941 cctggtgata gaaacatccg ccagcggtga agttgagaca ccatcagtat gaacaccaat
    18001 cgaatccaga ctattttcta cggtggtgat cacgccgaag ataccgatag aaccggtcag
    18061 ggtgctgggg ttagccacaa tgtaattagc tggcgtggaa atccagtaac caccagatgc
    18121 cgccatgccg cccatcgata caaccacaga cttacccgct gcccgggctg ctgccagttc
    18181 agcgcgaatc acttcagacg cggtaacgct gccgcctggg ctattaacac gcaggacaat
    18241 cgctttcact ttcgggtcaa ggcgagcgtc gcggatttgt gccgcagtgg tatcaccgcc
    18301 aacattcccc tgagtttcct cgccatccat aattgcgcca ttagcgaaga cgacaccgat
    18361 gctgtcaccg gtatctgccg gcgttttcaa tgcgtaatcg taataactga tggcgcgata
    18421 atttttatca gtcttactcc agccgaactc tttggtcaga gctttttcga tttcggcact
    18481 cgatgccagt gcatcgacca gcttgttttc cagtgcatat ttcgcggtat cgccaccggt
    18541 tttggttaaa ccctcaagca acccttgcgc gccagggaat acctgctgag cagggatctg
    18601 ccggttagcg gcaacagtat tcagatagtt ttgccacagc tcaccaatcc agcggctgtc
    18661 agcttcgcgg gctgccggtg acatatcatc acgaataaac ggttcaacgg cagatttata
    18721 cgtacccacg cggaacacat gggtggaaac tttcagctta tccagcaacg atttgtagta
    18781 cagaccgttg gtagcaaaac cgtgcagatc aaccacgcct tgcggtgaca gccaaatttt
    18841 attggcgaaa ctggcgagat aatattgccc ctggctgtag ttctcgccaa cggcataaac
    18901 cggtttcccg ctgtcacgaa actctttcag ggctttgccg atgtactgca tagacggctg
    18961 gtcgccgcct gcgaagtttt tcagatccat cacgataccg gtgatattgc ggtcgtcctt
    19021 cgcctggcga atggtgttga cgatatcaaa cagtgagttt tcctgcagac gatcggaact
    19081 ggcaccaagc agctggcggc ttaatttact aaaccgctga gaactgtctg gtttatcgac
    19141 gatcacacca gaaatgtcca gcagcagtgc gccacgactg gccgtttctt tcgaatcacc
    19201 accactgacc tgcatccaga tccccacacc aaccagtacg aggaaaataa agaacaggtt
    19261 aagtaccatt tcacggacga aattcagcag acgccacgtc catttaaaaa atccggcaat
    19321 aaatcgccaa agggttcgca tgtattctcc caacttaagg tcacacctgt cacaggcgca
    19381 atagatgtgg ttatcgtaat gacccaaccg gcacttgtca gcaggaattg cggactgtgc
    19441 tgtaacaaaa tccactctcg tgttaatttt gtgaataaat atcacgacag gagttaatca
    19501 aatggatgca ctcgaactat tgatcaatcg ccgtagcgcc tcccgcctgg ctgaacccgc
    19561 gccaacgggt gaacaactgc aaaacatcct gcgtgcgggt atgcgtgcgc cggaccataa
    19621 gtccatgcaa ccgtggcatt tttttgtgat tgaaggggaa gggtgcgagc gtttcagcgc
    19681 cgtactggaa cagggggcga ttgctgccgg tagtgatgat aaagctatcg acaaagcccg
    19741 taatgcgccg ttccgcgcac cgctcatcat cacggtggtg gcgaaatgca aagaaaatca
    19801 taaagtcccg cgatgggaac aggaaatgtc tgccggatgc gcggtcatgg cgatgcaaat
    19861 ggcagccgtt gcccagggat ttggcggcat ctggcgcagt ggcgcattaa ctgaaagtcc
    19921 ggtagtgcgt gaagcattcg gttgccgtga gcaggataaa attgtcggtt ttctctacct
    19981 cggcacgccg cagttgaaag catctacgtc gattaacgtc ccggacccga cgccgtttgt
    20041 aacttatttc tgataattgt cgcgaaactg tctggattgt gagcaaaggc gcaggaattc
    20101 agacactctc acttatcact tcacggaatg agggttacca tacccggatt gcattgacag
    20161 gagatgtcca tgagcgagaa ctcgattcgt ttgacccaat acagccacgg agctggttgc
    20221 ggctgtaaaa tttccccaaa agtgttggaa accatcctgc acagtgagca ggcgaagttt
    20281 gttgatccga atttgcttgt gggtaatgaa acccgcgacg atgcggcggt gtacgatctg
    20341 ggcaatggca ccagcgttat cagtaccacc gacttcttta tgccgatcgt tgataatcct
    20401 ttcgattttg gccgcattgc ggcgactaac gccatcagcg atatcttcgc gatggggggc
    20461 aaaccgatta tggcgattgc gatcctcggc tggccgatta acaaactttc cccggaaatt
    20521 gcccgcgaag taaccgaagg tggacgctat gcatgccgtc aggcgggtat tgcgctggct
    20581 ggcggtcact ccatcgatgc gccggagccg atttttggtc tggcggtaac ggggatcgta
    20641 ccgaccgagc gggtgaagaa aaacagtacc gcacaagccg gatgcaaact gttcctgacg
    20701 aaaccgctgg ggatcggcgt tcttaccacg gctgagaaaa aatcactgtt gaaaccagaa
    20761 catcagggac tggcgacgga agtgatgtgc cggatgaaca tcgcaggcgc gtcctttgcc
    20821 aacatcgaag gcgtaaaagc gatgaccgac gttacgggct ttggtctgct gggccacttg
    20881 agcgaaatgt gtcagggggc tggtgtgcag gcacgcgtcg actatgaagc gatcccgaaa
    20941 ctccccggtg ttgaagagta cattaagttg ggcgcagtac ctggcggcac tgaacgtaac
    21001 tttgccagct acggtcatct gatgggtgaa atgccgcgtg aagtgcgcga tctgctgtgc
    21061 gatccgcaaa cttctggcgg tttgctgctg gcggtcatgc cggaagcaga aaatgaggtt
    21121 aaaactacag ccgccgagtt tggcattgaa ctgacggcga ttggcgaact ggtgccagcg
    21181 cgcggcggtc gtgccatggt tgagatccgt taatttaatg cggttgttta ttgccgaaaa
    21241 accgagtctg gcgcgcgcca ttgctgatgt cctgcccaaa ccgcaccgga aaggcgatgg
    21301 ctttatcgag tgcggtaatg gtcaggtggt gacttggtgt atcggtcacc tgcttgagca
    21361 ggcgcagcca gacgcctacg acagccgcta tgcgcgctgg aatcttgcgg atttgccgat
    21421 tgtcccggaa aagtggcaat tacagccacg accctccgtg accaaacaac ttaacgtcat
    21481 caaacgcttc ctgcatgaag ccagcgaaat cgttcacgcc ggggacccgg atcgtgaagg
    21541 gcaattgctg gtggatgaag tgctggacta tctgcaactg gcaccggaaa agcgccagca
    21601 ggtgcagcgt tgcttgataa acgacctgaa cccgcaggcg gttgagcggg cgatcgaccg
    21661 tcttcgttcc aacagtgagt ttgtgccatt gtgcgtttct gcgctggcgc gagcgcgtgc
    21721 cgactggctg tacggcatca atatgacccg tgcgtatacc attctcggtc gcaatgccgg
    21781 ttatcagggc gtactttccg tgggacgcgt gcagacaccc gtgctcgggc tggtggtgcg
    21841 ccgcgatgaa gagattgaaa acttcgtggc gaaagacttc tttgaagtca aagcacatat
    21901 cgtgacacct gccgatgagc ggtttaccgc tatctggcaa ccgagcgaag cgtgtgaacc
    21961 gtaccaggat gaagaagggc gcttgttaca tcgtccactg gcggagcatg tggttaaccg
    22021 cattagtggt caaccggcta ttgtcaccag ctataacgat aaacgggaat cagaatccgc
    22081 gccgctgccg ttttcgcttt cggcgttgca gattgaagcg gcaaaacgct tcggtctgag
    22141 cgcgcagaac gtgcttgata tctgccagaa gctgtacgaa acgcacaagc taatcactta
    22201 tccgcgttcc gattgtcgct atttgccaga agaacatttt gccggacgcc acgcggtgat
    22261 gaatgccatc agcgttcatg caccggatct gttgccgcag ccagtggtag atccagatat
    22321 acgcaaccgc tgttgggatg acaaaaaggt cgatgcgcac cacgccatca tcccgaccgc
    22381 acggagttct gcgatcaacc tgacggagaa cgaagcgaag gtctataacc tgattgcccg
    22441 tcaatacctg atgcagttct gcccggatgc ggtgttccgc aagtgtgtta tcgaactgga
    22501 cattgccaaa ggcaaatttg tcgctaaagc ccgttttctt gctgaagcag gctggcgcac
    22561 gctgttaggc agcaaagagc gcgatgaaga aaacgacggc acgccattgc ctgtggtggc
    22621 gaaaggcgat gagttgctgt gtgaaaaagg tgaagtggta gagcggcaaa cccagccgcc
    22681 gcgccatttt accgatgcaa cactgctttc ggcgatgacc gggatcgcgc gctttgtgca
    22741 ggacaaagat ctgaaaaagg tccttcgtgc gaccgatggt ctggggacag aagcaacccg
    22801 tgccgggatt attgaactgc tgttcaagcg tggttttctg accaaaaaag ggcgctatat
    22861 ccactccacc gacgccggaa aagcgttatt ccattcgctg ccagaaatgg cgacgcgacc
    22921 ggacatgacc gcgcactggg aatcggtgct gacgcaaatc agcgaaaagc agtgtcgcta
    22981 tcaggacttt atgcagccgc tggtggggac gctatatcag cttattgatc aagccaaacg
    23041 tacgccggtg cggcagtttc gcggcattgt ggctccgggc agtggtggca gtgctgataa
    23101 gaaaaaggct gcaccgcgta aacgtagtgc gaaaaaaagt ccgccagcag atgaagccgg
    23161 aagcggggcg atagcgtaag cgagcgaatc tttcgtgcta ttcgagtcat attctgaaat
    23221 atccagcgga tcaagaaagt tcgttggata tttttttgca tggataaaat tatcgcctct
    23281 aaagtatgta ataacaggga atgtgatgaa aaaagtttta ttgcaaaacc atcctgggag
    23341 cgagaagtat tcttttaatg gctggggaat atttaatagt aattttgaac gggtgattaa
    23401 agaaaataag gccatgctgc tttgtaagtg ggggttttat ttaacatgtg ttgtcgctgt
    23461 aatgtttgta ttcgcagcga taacatccaa cggtttgaat gaaagaggcc tgattaccgc
    23521 gggatgctct tttctttatc tattaattat gatggggctt attgttcggg ccggttttaa
    23581 agcaaaaaaa gaacaactgc attattatca ggctaaaggt attgagccgc tcagtatcga
    23641 gaagttacag gcgctacaat tgatcgcacc ttatcgattc tatcataagc aatggtctga
    23701 aacgctagag ttctggccgc gaaagcctga acctggcaaa gataccttcc aatatcatgt
    23761 gcttcctttt gactcgatcg atatcatcag taaaagacgc gagtctttag agaatcaatg
    23821 gggtatcgaa gatagcgaaa gttattgtgc cttaatggag cattttcttt ctggcgacca
    23881 tggagccaat acctttaaag caaacatgga ggaagcccca gagcaggtta tcgccttgtt
    23941 gaataaattt gctgtttttc cctcagacta tatctctgat tgcgctaata atcgctccgg
    24001 taaatcctcg gcgaagctaa tatgggcggc ggaattatca tggatgatct cgatatcaag
    24061 cacagctttt caaaacggga caattgaaga agaactggcc tggcattata taatgcttgc
    24121 ttctcgaaag gcgcacgagt tgttcgaaag cgaagaagat tatcaaaaaa atagtctaat
    24181 gggatttctt tactggcata tctgctgcta tcgcagaaag ttaacggatg ctgaacttga
    24241 agcgtgttat cgttacgaca agcagttttg ggagcactac agtaagaaat gccgttggcc
    24301 cataagaaat gttccctggg gagcatcatc cgttaaatac tcataaacgc ctgaaatttt
    24361 gccgggggcg ctgcgcttga ccggcctaca ttagagcgta actgcaatga attgtgccca
    24421 tctgtaggcc tgataagcgt agcgccatca ggcatttaca acttaaatca caccctgcgc
    24481 cagcatcgca tcggcaacct tcacaaaacc ggcaatgttc gcgccctgca cgtagttggt
    24541 ttgctcacct tcaccaccat gctcaacaca ggcatggtgg atatccagca tgatgtgatg
    24601 caaacgtgcg tcaactttct cggctttcca gcccaggcgc gcagcgtttt gtgccatttc
    24661 caggcccgac gttgctacac cgccagcatt agccgcttta cccggtgcaa acagtacgcc
    24721 tgcctgctgg aacagttcag tcgcttcgat ggtggtcggc atatatgccc cttcagcgac
    24781 ggctttaacg ccattggcga taagctgatg cgcggcgtca acatccagtt cgttctgggt
    24841 ggcgcaaggc agggcgatat caaccggtac agaccacggc tgttggcctt cgagatagac
    24901 cagaccaaat tctttggcgt aatctgccgc tcgaccatcg cggctggctt tgatttcgat
    24961 aagacgtgcc agtttctctt tcgtgaatcc gctttcatca actacagtgc cgctggagtc
    25021 tgacgcagtg atcacacgag caccaaattc catcgctttt tcgatagcgt actgggcgac
    25081 gttgccggag ccagaaacgg aaacgctcat cccttcaaaa cccataccgt ggcgttttaa
    25141 cattgcttct gtgaaataaa ccagaccgta gccggtagct tccgggcgaa taagactacc
    25201 gccaaa
//
LOCUS       DAJTUI010000057        25046 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTUI010000057 DAJTUI010000000
VERSION     DAJTUI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.057"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..220
                     /locus_tag="OO464_002471"
     CDS             <1..220
                     /locus_tag="OO464_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HCR6227584.1"
                     /translation="TALAGTGEAAMTGEQILATHRSGKTEVYQRQAGFITGPAKVLML
                     TLTTQRPFDDHTDQLWTAWLTSFQPAKS"
     gene            242..649
                     /locus_tag="OO464_002472"
     CDS             242..649
                     /locus_tag="OO464_002472"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR6227585.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            646..2934
                     /locus_tag="OO464_002473"
     CDS             646..2934
                     /locus_tag="OO464_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6227586.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            2931..3920
                     /locus_tag="OO464_002474"
     CDS             2931..3920
                     /locus_tag="OO464_002474"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227587.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            4020..4223
                     /locus_tag="OO464_002475"
     CDS             4020..4223
                     /locus_tag="OO464_002475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227588.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            4241..4561
                     /locus_tag="OO464_002476"
     CDS             4241..4561
                     /locus_tag="OO464_002476"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227589.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(4627..4859)
                     /locus_tag="OO464_002477"
                     /pseudo
     CDS             complement(4627..4859)
                     /locus_tag="OO464_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(4969..5739)
                     /gene="yafV"
                     /locus_tag="OO464_002478"
     CDS             complement(4969..5739)
                     /gene="yafV"
                     /locus_tag="OO464_002478"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6227590.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            5920..6366
                     /gene="ivy"
                     /locus_tag="OO464_002479"
     CDS             5920..6366
                     /gene="ivy"
                     /locus_tag="OO464_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6227591.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(6409..8853)
                     /gene="fadE"
                     /locus_tag="OO464_002480"
     CDS             complement(6409..8853)
                     /gene="fadE"
                     /locus_tag="OO464_002480"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR6227592.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            9093..9671
                     /gene="lpcA"
                     /locus_tag="OO464_002481"
     CDS             9093..9671
                     /gene="lpcA"
                     /locus_tag="OO464_002481"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR6227593.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            9877..10644
                     /locus_tag="OO464_002482"
     CDS             9877..10644
                     /locus_tag="OO464_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6227594.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(10615..11355)
                     /locus_tag="OO464_002483"
     CDS             complement(10615..11355)
                     /locus_tag="OO464_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6227595.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            11739..12260
                     /locus_tag="OO464_002484"
                     /pseudo
     CDS             11739..12260
                     /locus_tag="OO464_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            12436..12933
                     /gene="rayT"
                     /locus_tag="OO464_002485"
     CDS             12436..12933
                     /gene="rayT"
                     /locus_tag="OO464_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR6227596.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(13150..14862)
                     /locus_tag="OO464_002486"
                     /pseudo
     CDS             complement(13150..14862)
                     /locus_tag="OO464_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            14834..15619
                     /gene="lafU"
                     /locus_tag="OO464_002487"
     CDS             14834..15619
                     /gene="lafU"
                     /locus_tag="OO464_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6227597.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            15690..16745
                     /gene="dinB"
                     /locus_tag="OO464_002488"
     CDS             15690..16745
                     /gene="dinB"
                     /locus_tag="OO464_002488"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6227598.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            16797..17090
                     /gene="yafN"
                     /locus_tag="OO464_002489"
     CDS             16797..17090
                     /gene="yafN"
                     /locus_tag="OO464_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR6227599.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            17093..17491
                     /gene="yafO"
                     /locus_tag="OO464_002490"
     CDS             17093..17491
                     /gene="yafO"
                     /locus_tag="OO464_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR6227600.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            17501..17953
                     /locus_tag="OO464_002491"
     CDS             17501..17953
                     /locus_tag="OO464_002491"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6227601.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            18199..19242
                     /locus_tag="OO464_002492"
     CDS             18199..19242
                     /locus_tag="OO464_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR6227602.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            19304..19918
                     /locus_tag="OO464_002493"
                     /pseudo
     CDS             19304..19918
                     /locus_tag="OO464_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(19975..21432)
                     /gene="pepD"
                     /locus_tag="OO464_002494"
     CDS             complement(19975..21432)
                     /gene="pepD"
                     /locus_tag="OO464_002494"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6227603.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            21694..22152
                     /gene="gpt"
                     /locus_tag="OO464_002495"
     CDS             21694..22152
                     /gene="gpt"
                     /locus_tag="OO464_002495"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6227604.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            22244..23488
                     /gene="frsA"
                     /locus_tag="OO464_002496"
     CDS             22244..23488
                     /gene="frsA"
                     /locus_tag="OO464_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6227605.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            23546..23947
                     /gene="crl"
                     /locus_tag="OO464_002497"
     CDS             23546..23947
                     /gene="crl"
                     /locus_tag="OO464_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6227606.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(23986..25041)
                     /gene="phoE"
                     /locus_tag="OO464_002498"
     CDS             complement(23986..25041)
                     /gene="phoE"
                     /locus_tag="OO464_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6227607.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         6090 a         6352 c         6657 g         5947 t
ORIGIN      
        1 aacggcactg gcgggaactg gcgaagctgc catgacaggt gaacagattc tcgcaactca
       61 tcgttctgga aaaacagaag tgtaccagcg tcaggccggg tttatcaccg ggccagcaaa
      121 ggtactaatg ttgacgctga ccactcagcg tccttttgat gatcacaccg accagctatg
      181 gactgcatgg ttgactagtt ttcagcctgc taaaagctaa aaaaaacagg gaggttttgt
      241 catgtatgaa gccgcacggg tggatgaccc catctaccac accagcgccc ttgccgggtt
      301 tttgataggg gccatcatag gtatcgccat tattgccgtc gccagctttg ctttttttac
      361 ctgcggcttt ttggccgggc tgattttgag ttttctggct gatcagatag cttccggagt
      421 cctgcaactg ggtgaggcta ttgggcgctc gatacattcc acagtgggaa aaattctgac
      481 cggttctgat aatgtcagca ccaacagtcg cccggccgcc cgggccgtac tgagtacggt
      541 gatatgcgaa gaccattccc cggaaatccg cattgcccaa ggttccggca acatctacat
      601 caacagccag ctgcccgtaa ggatgaccac actgagtgtg atgcagtgat tgaggacggc
      661 tcccccaatg tctttctcgg cggcggtacg cagacggtac tggaaatcag cccggaaatc
      721 cctgactggc tgcgtcaggt ggtggatgtg ctgtatgtgg tcgccggaat gcttggcggg
      781 ctggctggcg cctggcgtca ggcggcaaag atggggtcga aatttggtac aaaatgtgcg
      841 gcaaaattta ttggtggcga aatggtcggg atggccatct ctgacacagt catgggattg
      901 ttcagcaatc cggtagatgt caccactggg caaaaaattt tgctgccgga gacggatttt
      961 accctgccag gacgcctgcc tgtcacctgt tcgcgctttt acgccagcca cctggaaact
     1021 gaaggattgc tgggcagagg atggcggctg aactgggaga taaacctgcg tgaggatgaa
     1081 acatatatca ccttcatcgg tgtacagggt cgggaactga gttatccgaa agagatgctg
     1141 ataccgggcc accagatatt cgacccggaa gagcagtttt acctgagccg tctgcatgac
     1201 ggatgctatg tgctgcatta caccgactgc agctattacg tgtttgatga atttgatgac
     1261 cacggcgtcg cgccgttact ttttatggag acgccgtacc ggcagcgtat tgctttcggg
     1321 cgtgagaacg gcaggctggt gcgggtggcc tccagcagcg gacaccatct gttattgcac
     1381 cgcacgatga cgcaagcagg ggaacgcctg tcgcatattg aacttttgaa gggcgggcgt
     1441 cccggtaacc tggtggaata tcgttacgac gataacgggc aactgactgg cgtggtgaac
     1501 cgggctggtg tgacggtgcg ccagtttgcc tatgaaaatg gcctgatgac agagcatcgc
     1561 aacgcgacgg gctttacctg tacctaccac tgggaagaga ttgaaggttt cccgcgcgtg
     1621 gtggaacaca cgaccagcga cggcgaggat taccggttcc actatgattt tgctggcggg
     1681 cagacggtgg taaccggcag gccggagcag aagtggcagt ggtggtttga tgaagagacg
     1741 tatgtgactg cgcaccggac gccgggcggc ggaatgtacc gcttcacgta caacgaaaac
     1801 cattttccgg tagcggtgga actccccggc gaacgcaggg taacgctgga atacgacacc
     1861 ctgagccggg tggtgaaaga gacggacccg gcaggccggg tgacacagac gcagtggaac
     1921 ggcagttttg ctgaaatcac gcgccgggcg ctggatgacg accacgtatg gaaggcggat
     1981 tacaacgaac acggccaggt gatacgggag acggacccgg aagggcgcgt cacgcggtac
     2041 gggtatgatg atcaggggct gccggagacg gtctgccacc ccggtaaaca gcaggaccgt
     2101 tacacctgga acgcgctggg gttactgagc agccaccgtc gcatcacggg cagtgtgcag
     2161 tcctggcagt atacgcagcg cgggatgctg gcgcgccata cggatgaaga gaaacgggag
     2221 acgcgctggc agtacacccc ggaagggctg gttgccagtc tgagcaacgg caacggggct
     2281 cagtaccggt tcagctacga cggcgacgga cggctgacgg gagaacagcg cccggacggc
     2341 ctgataagga tgtttgcgct gaatgcggac gggtttccgg tcatcatccc gacgcagggc
     2401 acagagggcg gggtgcgcaa tgaacagcag gagcgtgatg cgctgggccg cctgctgcgc
     2461 agtgacacgc agcacagtac acggaccttc agctacaacc gtcttgacca gataacggaa
     2521 gtgacgctca cccccacgga agaaggggaa cggctgcacc acatgcaggc ggacacggtg
     2581 cgttttgcgt atgaccgcag cgggtggctg acggcagagc acagcgtgca cggcagtatc
     2641 aaataccggc gcgatgcgct gggcaatccc accgatatca cgttgccgga cgggcagcat
     2701 ctcagtcatc tttactacgg cagcgggcat ctgctccaga cggcactgga cggtatcacg
     2761 gtcagtgaat atgagcgtga cagcctgcac cgtcaggtga tacgcacgca ggggaaactg
     2821 gcgacgttca gcggctacaa cgccgacaac cggttaagct ggcagcgcag cctgccaggt
     2881 ggcagccaga atccacagca ggcggtactc ccccggcgca gaacgacagc gtgacggcgc
     2941 gggactatat ctggaacgca gacggtgaag tcggcggcat caatgacaaa ctgcgcggtt
     3001 gcctggtgtt cagctatgac cgcagcggct ggctgaccag ccgcacgggg cagatgtatg
     3061 accatgacca ttattattac gacaaagcgg gcaacctgct gacggatgag tatcagggag
     3121 cggtgatgga taaccgcctg ccgggttacg ggcgtgaccg ctaccgctat aacgaatggg
     3181 gcgaactgac agagcgcagg gaccagcagc tggagtggaa cgcgcagggc cagttaacgc
     3241 gggtcatcag cagcaacagc gagacgcggt atcagtacga tgcgctgggc aggcgcatca
     3301 gcaaggcgac cagcaatctc cataccgacc ggggagaacg aagccggaca acgtttgtgt
     3361 gggaagggtt ccggctgtta caggagacaa cgtggcaggg aaagcggaca tacctgtacg
     3421 atgcggaaca gccgtacacg ccggtggctg ccatcacggg gcgcggagag agccagaaaa
     3481 tctggtatta ccacacggac ctgacgggca cggtacacga ggtaaccgcg ccggacggga
     3541 cgctggtgtg ggcgggctac caggcgggat ttggtgaaaa ccggggcgac atcagcaaca
     3601 gcggggcgta ctttgagcag ccgcagcggc tgccggggca gtactttgac gaagaaacag
     3661 ggctgcatta caatctgttc aggtattatg cgccggagtg cgggaggttc gtaagccagg
     3721 acccgatagg attaaacggt gggttgaact tatatgctta tgcgcctaat ccgttaggtt
     3781 ggatcgatcc gctaggactg aattctttag gaatagattc aaataatata tttagaggag
     3841 atggtaacta taaaggtggc ggaattggac agccaaagag tggcacaata tcagcacagg
     3901 acattattga tcatctctaa gaaaaaggtg atgggcgttt aacatctttt agtactaaaa
     3961 aaacatctag ttccaactct cgtggtgcag attttttctc aaaaaaacat gtcttcaaag
     4021 tggcagtttc tgatctcgat gatttagtta aaaataatca gatcactgtt ttaacacaag
     4081 aaaaagtaaa agcaatattt ggtttagaat caagcaagaa aattagaaaa gaacttggta
     4141 atattcttca gaatatggct aggaataatg aggtgttagt tcatgggcaa attcttggaa
     4201 ataaagttag ccgatgtaat tagttagtgg agaggaagtt gtggacgaaa ttagaataag
     4261 aattgctggt aatacggctg cgccttgttt ccatgtgcta aaaaataagg gatataaagt
     4321 aacaatggaa actcatgcat tatctgacga ttgtttagat tgtgtatatg attggtatgc
     4381 ggaaaaaaat ggacaatttt ttagtgcaac gagtccagaa gagttacttg gattagttgc
     4441 aatgtgggaa gttcgtggta atgactggag agctaatttg aatgagataa atacttatca
     4501 aaaagccata gaagaatccc cgttttttga taaaaacggc aatgaaatag agtctgacta
     4561 aattttattg atatagcaac gataataacc ggaaatttct tccagatctt tccggctttt
     4621 tattatttaa ctttcgttca gcacactaaa catcccgtct cgcatcccct taactggctg
     4681 tttaacctga taaagttccc tgcgcagttc ctgtacttcg ttgttatccc aagcaggatc
     4741 aaacatccct ccgagatctt cacggtaacg cccgtgccga tctcaaatcc cgcttttctc
     4801 agccatttgg cgctgatttt gatcaccggc gtgtcgctgc ggttgggcag ataacccacg
     4861 gtacagcgac gctgtgtttt ggaaatggtg acttctggcg taatattgtg tttaagttag
     4921 gggctttcgt aatctagccc agcccccggc cctaaacaca acccccactc accacaacct
     4981 aaactcatcc gcatcccgcc atgccggaaa cttttctcta tattcccgca atgccaccat
     5041 cgacagctcc gcatcaatac gcgttgcctt atgcgcgtcg gcggtagcga taatctcgcc
     5101 ttgcggatta atcacccggc tgtcaccgcg ataatggcag ccgttgccat cgctgccgac
     5161 gcgattgcat cccgccacat acgcctgatt ctcaatcgcg cgggccgtca gcaatgcctg
     5221 ccagtgcaga gagcgcggag ctggccagtt ggcgacgtac agggcgaggt cataatcgtt
     5281 gagattgcgc gaccacactg gaaaacgtaa gtcgtagcac accagcggca aaatacgcca
     5341 gccgcgccat tccacaatca ctcgcgcatt gcccgcttta taatgcagat gctcatctgc
     5401 catgcggaac agatgacgct tatcataaaa atgtaccgtg ccgcccggct caaccagcaa
     5461 aaagcggtta accgaaccag actccgtttg taatgcaaca ctgcctgcaa tcagcgcatt
     5521 gcactgctgc gccttagctg tcatccagtt cactacgtca tcttgtgcaa gcgacgaagc
     5581 tgccgcttcc atggcaaagc cgctggtaaa catctccggt agaacgatca catcgcgccc
     5641 ggtaatacct tccagttgac gatcaaaatg gcgcaggttg gcaggaccat ccacccacac
     5701 caacggttgc tgcaaaagcg taatcttcaa actaggcacg gtgtacaact cctttatgcg
     5761 aagggtttta taactttaac accttatcag gcagttgcct tagcgcagaa taaattgata
     5821 acaaatgctg atattggaaa tatctgattt gcaaattatc gtgttatcgc taggctttag
     5881 gaggttaata acatgggcag gataagctcg ggaggaatga tgtttaaggc aataacgaca
     5941 gtcgccgcac tggtcatcgc caccagtgca atggcgcagg atgatttaac cattagcagc
     6001 cttgcaaagg gcgaaaccac caaagctgcg tttaatcaga tggtgcaagg gcataagcta
     6061 cctgcatggg tgatgaaagg cggtacttat actcccgcac aaaccgtaac gttgggagat
     6121 gagacgtatc aggtgatgag cgcgtgcaaa ccgcatgact gtggctcgca acgtatcgct
     6181 gtgatgtggt ccgagaaatc taatcagatg acggggctgt tctcggctat tgatgagaaa
     6241 acgtcgcaag agaaactcac ctggctgaat gtgaacgatg cgctttcgat tgatggtaaa
     6301 acggtgctgt tcgcggcgtt gaccggcagc ctggaaaacc atccggatgg ctttaatttt
     6361 aaataattag cggataaaga aacggagcct ttcggctccg ttattcaatt acgcggcttc
     6421 aactttccgc actttctccg gcaactttac cggcttcgtc gccagctctt ccggatcaaa
     6481 gtcatcaacg ttaatactgc gcagacggct ttcttccgct ttcaccagaa tagcggcttc
     6541 atctttatca atcagcccct tcgtcagcgc gttgtgcgcc agttcatcca gacgggtaaa
     6601 tggcagattt ttacccagct ctttacagat ccgctgatga atcgggtcgg cggcaatcac
     6661 atccaccaac gcctcttcca gtaaaccaac cgggttatgc tcgcttggcg tcaggtactg
     6721 accgcgacca atacgtgaac gagtggcgtt cggcacttgt aaaatcttcg ccattttatg
     6781 atccagcttg tcagacggtg ccagataatg acgtccggtc gggaagatca ccacattcag
     6841 cagcccggca accacgcggt tcgggaagtt ttgcagtaaa ttgtccattg cctgttctgc
     6901 ctgatacagc gcatcctgta cgccccagtg caccagcggc agatcggctt cattacggcc
     6961 ttcgtcgtca taacgcttca gaacggcaga ggcgaggtaa agctggctta aaatatcccc
     7021 cagacgtgcc gagatacgct cacgacgttt caggctgccg cccagcagtg ccatcgagac
     7081 atcagaaagc agggcgaggt tggcgctcag gcggttcagg tgctgatagt agcgtttagt
     7141 ggcatcgccg gttggcgtgc tgctggttaa accgcgcgtc aggcccagcc agaagctgcg
     7201 aactttgttg ctaccgacgt gaccgatatg tttgaacaac agtttatcga acgcgttgac
     7261 gtcattgttc ttcgccgctt ccatctcttc cagcacgtac ggatggcaac gaatcgctcc
     7321 ttgtccgaag atcatcatgc tgcgggtcag aatgttagcc ccttcaacgg tgatggcaat
     7381 cggtgcgccc tggtaagcac gcgccaggaa gttgctttgc ccgagcataa tgcctttacc
     7441 gccggtaata tccatcgcat caataatcga ctgctgcccg cggtgggtac agtgatactt
     7501 aacgatagcc gacagcacgg caggtttttc gccgagcata atgccgtagg taatcagcga
     7561 tgccgcagca tccattacat aagcgttccc ggcaatacgc gccagcggct cttcaatccc
     7621 ttccatctta ccgatagaga ttttgaactg acggcgaatg tgagaatacg cgcccgttgc
     7681 cagcgctacc gatttcacgc cgccggttga gttggaaggc agggtgatgc cgcggcctac
     7741 cgagaggcac tccaccagca tccgccagcc ttgcccggcc attttcggcc cgccgatgat
     7801 gtaatcgatc ggcacgaaga catctttacc gcgcgtcggt ccgttctgga acggtacgtt
     7861 cagcgggaag tggcgacgac caatttccac gcccggcgtg gtggttggga tcagcgcaca
     7921 ggtaatgcct aaatcttctg caccgccgag taatttttcc gggtcggaga gtttaaacgc
     7981 cagcccaagc acggtcgcaa tcggtgccag cgtaatgtag cgtttgttcc aggtcagacg
     8041 catccccagc acctgctggc cctgccattc gcccatgcag acaatcccgg tgtccggaat
     8101 cgcgcccgca tcggaacccg cttccgggct ggtcagtgca aagcagggga tctcctgacc
     8161 acgcgccaga cgcggcagat agtgatcttt ctgctcgtca gtgccgtaat gttgcaacag
     8221 ttcgcccggg cctaatgagt ttggcacgcc gacggtaatc gccaggatcc cgctcacgcc
     8281 agagagtttt tgcagcacac gagactgggc ataagctgag aactccagtc cgccgtactc
     8341 ttttttgata atcatcgcga agaagcgatg ctctttaagg tacgcccaca actccggtgg
     8401 cagatccgcc agctcatgag taatctggaa atcattcgcc atccggcagg cttcttctac
     8461 cgggccgtcg agaaacgctt gctcttcagc ggtcaggcgc ggctgcggat agttatgcag
     8521 ctttttccag tccggcttgc cctggaacaa gtcgccctcc caccaggtgg tgcccgcatc
     8581 aatcgcttct ttctcagtgc gcgacatcgg cggcatcacc ttacggaaac cgcgaaatac
     8641 cggcgcggaa atcatcgact tacgcatagg cgcaaagtta aatggcacga ggataatggt
     8701 cagaggcacc agtacccacg ccgaccacag accagcaacg ccgagggcgg ctgtccaggc
     8761 gagcaaaatc agactgctga taaataagct cacgcggtga tagaacaacg cgccgagcag
     8821 gacaaccgta gcgagaatac tcaaaatcat cataacgaaa agccccttac ttgtaggagg
     8881 tctgaccact tgtgatgata tggttgtagt ggatgtaaaa acatttagca atgtgtttac
     8941 aatataatta caacaaagct cacattgttg ctgtttttat ccgcacttca ggtcaaaaag
     9001 tcctggtcat agcacctgcc cgtacttctc gcttttggcg gtatccggta cactgcattt
     9061 tgtctattac atttatgctg aaggatatcc tcatgtacca ggatcttatt cgtaacgaac
     9121 tgaacgaagc ggcggaaacg ctggctaact ttttaaaaga tgacgccaat attcacgcca
     9181 ttcagcgcgc ggcggtcctg ttagcagaca gctttaaagc cggtggcaaa gtgctttcct
     9241 gcggcaacgg cggttcccat tgcgacgcta tgcactttgc cgaagagttg accggtcgct
     9301 accgtgaaaa ccgtccgggc tacccggcga ttgctatttc tgacgttagt catatttcct
     9361 gcgtcggtaa tgatttcggt ttcaatgata ttttctcccg ctacgttgaa gcggtaggtc
     9421 gcgaaggcga tgtactgctg gggatctcca cctccggtaa ctctgcaaac gtgatcaaag
     9481 cgatcgcagc ggcgcgtgag aaggggatga aagtgatcac cctgaccggt aaagacggcg
     9541 gtaaaatggc tggcacggcg gatatcgaaa ttcgcgttcc gcactttggt tatgccgacc
     9601 gcattcagga gattcacatt aaagtgatcc atatcctgat ccagttaatt gaaaaagaga
     9661 tggttaagta agtctggcgt aggccggata aggcgtttac gccgcatccg gcacttgtgc
     9721 gctgatgcct gatgcgatgc tgacgcgtct tatcatgcct acaaatctgt acccgaaccg
     9781 taggccgaat aattcgttta cgccgcatcc gacctgaaaa ttcttaaatc aatcttcgcc
     9841 ggggtccatg cgctcccgct gttgtggagg ttacccatgt gcgaattgct cgggatgagc
     9901 gccaacgtcc ctaccgatat ctgctttagt ttcaccggac ttgtacagcg tggtggtgga
     9961 accgggccac ataaagatgg ctggggcatt accttttacg aaggtaaagg ctgtcgcaca
    10021 tttaaagatc cacaacccag ctttaattcc cccatcgcca aacttgtcca ggactacccg
    10081 ataaaatcct gttcggtggt ggctcatatt cgccaggcta atcggggcga ggtggcgctg
    10141 gaaaatactc acccatttac ccgcgagtta tgggggcgta actggactta tgcccataac
    10201 ggacaactga cgggctacaa atcactggaa accggcaact tccgcccggt cggtgaaacc
    10261 gacagcgaaa aagccttttg ctggctgctg cataaattaa cgcagcgtta cccgcgcacg
    10321 ccgggcaaca tggcggcagt gtttaaatat atcgcctcac tggcggatga actgcgggcg
    10381 aagggcgttt tcaacatgct gctttcggac gggcgctatg taatggcgta ttgctcgact
    10441 aatttacact ggatcacccg ccgcgcgccg tttggcgtgg caacgttgct ggatcaggat
    10501 gtggaaatcg acttcagctc gcagaccaca ccgaatgatg tggtcacggt gattgcgaca
    10561 cagccgctga cgggcaatga aacctggcaa aaaattatgc caggcgaatg gcgcttattt
    10621 tgcctcgggg agcgtgtagt ttgatgccag ttgtggctgc accacttcgt ggcttaacgg
    10681 cttgctgacc acgtaacggc cattggccac agaaacggtc ggtggcttac gggtttgctc
    10741 aaagtagtcg tagcccggct tcagctgctc ccagaagtcc ttaaagttgg aatatttatg
    10801 gcgcttcata ttggcgtcgg tcatgcggaa tgggtaaata ctcacctgca cgctcggctg
    10861 accaaacacc agcgcaccag taacgaactg gaagatctca tcaataccct gattggtcat
    10921 tgcgtagcag ccgatggata cacaatcgcc gtggatcatc aggtatttcc cttcataacc
    10981 atgcgcacgg tcataagcat tggggaatcc aatattaatc gctttgtagt aacggctgtc
    11041 tggttttaac tgattacgct ggacgctata aaacccttcc gggcttttga aatcgccctg
    11101 acgctgtttt ggccctaagc cgccggaata tttacagatt ttataactgt cgagcagttg
    11161 atattgctcg cccattttga cgtaaagatc gagcgtacgt tcttccttga agatctggat
    11221 gtagacaggg gatcccatca actgctgctt atactctttg ctcactggcg tggtcgagct
    11281 actgctgccc agcaaaccgg caaacgaaac gcacgggatc aacaacatcg caagaattaa
    11341 tgcgatttta cgcatactgc ttattccttg ataaaacggt tacacacgcc aggacggcaa
    11401 aatggatccc aaatcggaat agtctggatt tggaaggctc acattatcac caaaagagtt
    11461 ttacgcaagc ctgtcggcgc tgggtttaca aatttagcac aggggcagtt ttcaatagtc
    11521 cgaaaagcgc atgtatcatt tatttcctaa tattatgaaa tctcaatagt aatatgcaaa
    11581 taagcaatta ataatcccgt ttactcctcc ggtcttttaa aattctcacc ttcattcagc
    11641 cggtttatgt ctttcatgtc ttcgttcttg ctcggcagat ttttgcatcc aggcgtgttt
    11701 tcgttatgcg ttttgctccc tttactcgcc agcgccacca caaccgggag cagataaaac
    11761 agcatccaga atggtttcct gcgccgctca aggcgagtga cagacgctgg caggcgctgg
    11821 cggaaaacaa ccacttttta agcagcgatc acctgcataa catcaccgaa gtggcgattc
    11881 accgcctgga gcagcagctt ggcaagcctt acgtctgggg cggcacgcgg cccgatcaag
    11941 gcttcgattg tagtgggtta gttttttatg cctacaacaa gatccttgag gcgaaactcc
    12001 cgcgcacggc caatgagatg taccactacc gtcgggcaac gattgtggcg aataacgacc
    12061 tgcgccgggg agatttgcta tttttccata tccacagccg cgagattgcc gaccatatgg
    12121 gggtgtatct gggcgacggt cagtttattg agtcactgcg caccggggaa accattcggg
    12181 taagccggtt agccgaacct ttctggcagg accatttttt aggcgcgcgc aggattttga
    12241 cggaagagat gattttgtag gccggataag gcgtttacgc cgcatccggc ggttgtgcac
    12301 ggatgcctga tgcgacgctg acgcgtctta tcatgcctac aaatctgtgc gtttttgcga
    12361 gccgctttcc cgatataaca cagcgtcatt gtcggccttt ccttttcctc ttaccgttga
    12421 gaaaaaggag tcgctatgtc ggaatatcgt cgttattaca tcaagggggg aacatggttt
    12481 ttcacggtaa atttacgcaa tcgtcgaagc catcttctga ccacccagtt ccagacgctc
    12541 cgtaacgcca ttattaacgt taagcgagac agaccttttg aaattaacgc ctgggtcgtt
    12601 ttgccggagc atatgcattg catatggaca ttacccgaaa gcgatgatga tttttcctct
    12661 cgctggcggg agattaaaaa gcagttcacc catgcttgcg gattgaaaaa tatctggcaa
    12721 ccgcgttttt gggagcatgc cattcgcaat acaaaagatt atcgccatca tgttgattat
    12781 atttatataa atccagagaa acatggctgg gtaaagcagg tgagtgattg gccattctca
    12841 acgttccatc gcgatgtcgc gcgagggtta tatcccatcg attgggcggg agacataacg
    12901 gatttaagtg ccggggagcg tatcatttta taactgtgcg ctggtgccgg atgcgcttac
    12961 gcttatccgg cctacaaatc gtgctttaac ggtaggccgg ataaggcgtt cacgccgcat
    13021 ccggcagtta tgcgcagatg cctgatgcga cgctggcgcg tcttatcatg cctaaaaact
    13081 tgtgcctgaa cggtaggccg gataaggcgt ttacgccgca tccggcaatt gtgcaccgat
    13141 gccggattct tacatcaacg cccccataat cttcaactcc agctcatccg gcacttcgtt
    13201 atacgacagc acatgcagcc ccggcgcaaa caaccttgca taacgcggca gcaaagggcg
    13261 tagctgcggc ggcaccagca gcaccggatc tttccccgcc gctttcatct gctctttcac
    13321 ctgtggcatc gtgctctgga actggttgag catattcggg tcaaccggca cgctgtcgag
    13381 catcactttg ccggcctgtt gcgcctgatt cactacgttg gtcagcagat tttccagctc
    13441 attattcagc gtatacaccg tcagctcctg cttgcgaacg aacggatgag taatgctgcg
    13501 ccgcagcgcc aggcgcacat cggccgccag cagaatatga tctttagtca ccgtgctact
    13561 ggcgaccagc acggtggcga tggtgacgat atcgcgcagg gaaacgcctt cggtcagcag
    13621 cgcccggtac actttcagca attggctgta attgagcgcc gcgcttaaat cctccgccag
    13681 gcgcggtgcc atcgacgaca aacggttatg caactgcgtg atgtcatcat agttaaacaa
    13741 atcaggaata tagctgcgca caatcttgtt cacatgggtg gcaatcacac tggcgctgtc
    13801 gatcacctga taccccatat tcagagcctt cgctttctgc gcaggctgga tccaggtgac
    13861 cggtatcccg tacgccgggt cgttccccag cacgccgtca atctcgccgt aggtttcgct
    13921 ggagggcagc gccatcagtt tatccgccgg aatatccgct tcatcagcct caatgccgtt
    13981 gatgaaaata gcgtactggc tgggcttgag gcggaagttt tcccgaatgc ggatctccgg
    14041 cagcagcacg ccgttaccgt cggaaatcac ctgccgaacg ccgcgaatcc gctgggtgag
    14101 cgggttgccc tgcgctttgt ccaccagcgc caccagcttg tagccgaggc ttaagctgat
    14161 gggctcgatc agcggaatag tttcccaact gacctgttgt tcgctggttt cagtgatggt
    14221 gcgggtcagc gtttcgaggc ttttctcttc cgcctccgcc gcctgcgggc gtttgctcat
    14281 ccgccagccg gtaaagccaa gcagggtgct gaacagcaaa aacggcagat gcggcatccc
    14341 cggcaccacc gccagcacga acatgatccc ggtggcggtg tagagcaccg acgggcttgc
    14401 cagcaactgg tggcgcacgt catgggcgat atcgccgttg tcgctgacgc gggtgacgat
    14461 aatcgccgcc gcggtggaga gcagcaggga agggatctgc gccaccaggc cgtcgccgat
    14521 ggtcatcagc acgtattgct ggaaggcggc gtcggcgctc aggttgtatt tgaagatgcc
    14581 gatacagacg ccgccgctca ggttgatcgc cagaatcatc atcccggcga tggcgtctcc
    14641 gcgtacaaac ttcgatgccc cgtccatcgc gccgtagaaa tcggcctcgc tggcaacatc
    14701 tttacgccgg gtttgcgcct gcgcctggtt gatcaatccg gcgttaagat cggcgtcaat
    14761 cgccatctgt ttgccgggca tcgcgtccag ggtaaagcgg gcagaaacct cggaaatacg
    14821 ctcggcctct ttggtgacga cgataaagtt gattgtcaac agcgtcagta aatccgagcg
    14881 tgaaagcatt atcgccgcgc tgcacgggca gtcgattttt aacggtggtg ggctgtcgcc
    14941 gttaaataaa atcagcccgt ctcatccgcc aaaacctgcc accgtggcgg tgccggaaga
    15001 gacggagaaa aaagcgcgtg atgtgaatga gaaaacggcg ctgctgaaga agaaatccgc
    15061 caccgagctt ggcgagttgg caaccagtat caacaccatt gcccgtgatg cgcatatgga
    15121 agcgaatctg gagatggaga ttgttcccca gggattacgc gtgctgatta aagacgacca
    15181 gaatcgcaat atgttcgaac gcggcagcgc gcagattatg ccgttcttta aaactttgct
    15241 ggtggagctc gcgccagtgt tcgactcgct ctataacaaa attattatta ccgggcatac
    15301 cgatgcgatg gcctacaaaa acaatatcta caacaactgg aacctttcgg gcgatcgcgc
    15361 gctttcagct cgtcgggtgc tggaagaggc cggaatgccg gaagataaag tgatgcaggt
    15421 aagcgcaatg gcggaccaga tgctgctgga cgcgaagaat ccgcaaagcg cgggcaatcg
    15481 gcgtattgag attatggtgc tgaccaaaag tgcgtccgat acgctgtatc aatactttgg
    15541 tcagcatggg gataaagtgg tgcagccgct ggtgcaaaag ctggataagc agcaggtgct
    15601 ttcgcagcga atgcgctaaa tgctgaatct ttacgcattt ctcaaaacct gaaatcactg
    15661 tatactttac cagtgttgag aggtgagcaa tgcgtaaaat cattcatgtg gatatggact
    15721 gctttttcgc agcggtggag atgcgcgaca atcccgctct gcgcgatatc cctattgcta
    15781 ttggcggcag ccgcgaacgt cggggggtga tcagcaccgc caattatccc gcgcgtaaat
    15841 ttggcgtacg tagcgctatg cctactggga tggcgctcaa attatgccca catctcacct
    15901 tgcttccggg gcgctttgac gcctacaaag aagcctcaaa tcatatccgt gaaatcttct
    15961 cgcgctacac ctcgcgcatt gaaccgttgt cactggatga ggcttatctc gatgtcaccg
    16021 atagcgtcca ttgccacggt tctgcgaccc tcatcgccca ggaaatccgc cagacgatct
    16081 tcaacgagct gcaactgacg gcatctgcgg gcgtggcacc agtaaagttt ctcgccaaaa
    16141 tcgcctccga catgaataaa cccaacggtc agtttgtgat tacgccggca gaagttccgg
    16201 catttttaca aaccttacca ctggaaaaaa tccccggcgt cggcaaagtc tcggcggcaa
    16261 aactggaagc gatggggctg cggacctgcg gtgatgtaca aaagtgtgat ctggtgattc
    16321 tgcttaaacg ctttggcaaa tttggccgca ttttgtggga gcgtagtcag gggattgacg
    16381 agcgcgacgt taacagcgaa cggttacgaa aatccgtcgg cgtggaacgc acgatggcgg
    16441 aagatattca tcactggtct gaatgtgaag cgattatcga gcggctgtat ccggaacttg
    16501 aacgccgtct ggcaaaggta aaacctgatt tactgattgc tcgccagggg gtgaaattaa
    16561 agttcgacga ttttcagcag accacccagg agcacgtctg gccgcggctg aataaagctg
    16621 acttaatcgc caccgcgcgt aaaacctggg atgaacgccg cggcgggcgc ggtgtgcgtc
    16681 tggtggggct gcatgtgacg ttgcttgacc cgcaaatgga aagacaactg gtgctgggat
    16741 tatgatgtat actattatgt atattctggt gtgcattatt atgagggtat cactgtatgc
    16801 atcgaattct cgctgaaaaa tcggtcaata tcactgagtt acgtaaaaac ccagctaaat
    16861 actttattga tcaaccagtt gcggttcttt ctaataatcg ccccgcagga tatctcttaa
    16921 gtgccagcgc attcgaagcg ttaatggaca tgcttgctga acaagaggag aaaaagccca
    16981 taaaggcgcg ctttcgtcca agtgctgcaa gattagagga aattacacgc cgcgctaaac
    17041 aatatcttaa tgatatgacg gatgatgatt tcaatgactt taaggaataa ggatgcgggt
    17101 attcaaaaca aaacttattc gcctgcaact tacagcagag gaacttgatg cgttaacggc
    17161 ggattttatt tcctataagc gtgacggtgt tttgccagat atatttggtc gcgatgcact
    17221 ctacgacgac tcgtttacct ggccattaat caaatttgag cgagttgctc atattcatct
    17281 ggcaaatgtg aataatccat ttccgccaca gttgcgccaa ttcagcagaa cgaatgacga
    17341 agcgcatttg gtatattgtc agggtgcgtt tgatgagcaa gcatggttgc tcattgccat
    17401 tctgaagcct gaacctcata aactggctcg agataacaac caaatgcata aaattggaaa
    17461 aatggcagaa gcgtttcgca tgcgtttttg aatttatatt atgaataaca tacaaataag
    17521 agattatcag cctggcgatt ttcagcaact atgcgctatt ttcattagag cggttacgat
    17581 gaccgtcagt cagcattatt cactacaaca aattgccgcc tgggcgcaga ttgacgaatc
    17641 tcgctggaag gagaaactcg cgaaatcaca agtgcgggtt gcagttatta atgcaaaact
    17701 ggtcggcttt attacctgcg ttgaacacta tatcgatatg ttatttgttg accctgaata
    17761 cacccgccgt ggggttgcca gcgctttgtt aaaacctttt attaagtctg aatccgaact
    17821 tacggtggac gcaagcataa ccgcaaaacc cttttttgaa cgttatggtt ttcagacagt
    17881 taagcagcag cgcgttgaat gccggggagc gtggtttatt aatttttata tgcgttataa
    17941 accgcaacat taaatccagc ttgcaatgaa aataacgccc gcctagtatg tgcgcgtttt
    18001 cttattccac aaactgcaag gaggtaaatc atgacaaatc ctttatcaat gactctttgc
    18061 agacctttcc aggattaatt ctttttttct tgccctggat tcgtctgcca tttcctgatt
    18121 ttttaattgt tactctcaat aattcaagtc gcagcacatg caacttgaag tatgacgagt
    18181 ctatttacgg aatggtatat gggcaattat attcgtccct tatccgatgc ggtatttacc
    18241 atcgcatctg atgacctgtg gatcgagagt ttagcgatcc aacaattaca caccacggca
    18301 aatttaccca acatgcagcg cgtagtgggg atgccagatt tacaccccgg acgcggctac
    18361 ccgattggcg cagcgttctt ctccgcaggc cgtttttacc cagcactggt cggcaatgat
    18421 atcggctgtg gcatggcgct atggcaaaca gatattctcg ctcgcaaata caacgccgat
    18481 aagtttgaaa agcgattatc agctttggat gacgttgctg aagaaagctg gctggaggaa
    18541 aacctgccgt cagcatttgc acagcatccg tggcgcagct cgcttggctc tatcggtggt
    18601 ggcaatcatt tcgctgaact gcaacaagtt gatcaaatca tcaacgctga actgtttgca
    18661 ctggcaggtc tggatgcgca gcatctgcaa ctgctggttc atagcggctc gagggggcta
    18721 gggcagtcta ttttacagcg gcatattgcc tcgttttcgc atcatggttt gcctgaaggc
    18781 agtgacgacg cgctggcgtt tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg
    18841 caacaggtta aggccaccgg aagtccggtt ctggatgtgg cgcataactt tgttagcgcg
    18901 tgtcgaatcg gtgatcaaca aggggtctta catcgtaaag gtgccacgcc tgatgactgt
    18961 ggtctggtgg ttattcccgg ttcacgcggt gattactcca ggctggttca gcccgtcagg
    19021 agtgaggaaa cattgcattc gctggcgcat ggggctgggc gtaaatgggg gcgcaccgag
    19081 tgtaaagggc gtctggcagc gaaatacaca gcgacgcaac tctcgcgtac tgaacttggc
    19141 agtcgggtaa tttgtcgcga taaacaactc atcttcgaag aagcgccaca agcctataaa
    19201 tcggctgaaa gtgtggtgca atgtcagggc aggccggatt aattattcct gtcgcgcggc
    19261 tgcgtccggt gctaacgctc aaaaacagtg gagggaaaaa aggatgatct tgctataact
    19321 ctcctcagcg caggggccgg aagaatgttg tctcgcagtg aaaaaggcgc tggacaggct
    19381 aattaaagaa gctgcccgac aagacgtcgc ggtaacggtg ctggaaacag aaacaggccg
    19441 ctactctgac acgctgcgtt cggcgctgat ttctctggat ggcgacaacg catgggcgtt
    19501 aagcgaaagc tggtgtggca ctattcagtg gatttgcccg agcccgtatc gcccacatca
    19561 cgggcgtaaa aactggtttc tgggcattgg gcgttttacc gctgatgagc aggagcaatc
    19621 cgatgcgatc cggtatgaga cgctgcgttc gtcggggccg ggcggtcaac atgtcaataa
    19681 aaccgactcg gcggtacgcg ccacgcattt ggcatccggt attagcgtga aggttcagtc
    19741 agagcgcagc cagcatgcta acaaacggct ggcgcgattg ctgattgcct ggaagctgga
    19801 tcagcagcaa taggaaaata gcgcggtgct gaaatcacag cggcgaatgt tccatcacca
    19861 gattgaacgt ggcaacccgc gacgaacgtt tacagggatg gcttttatcg aaggataatg
    19921 aaggatgaaa ctgccggaag gcgattaaac gccatccggc agcaaatcaa ataattactt
    19981 cgccggaatt tctttcagca gttcagtcag cagtgtccag taatgaccta cgctttcgat
    20041 gtgaacttgc tcatccggag agtgtggacc ggtgatagtt ggcccgatag aaaccatgtc
    20101 catttccgga tacggttttt tgaacagacc acattccagg cccgcgtgga taatctggat
    20161 gttcggcgtc ttgttgaaca ggcgctgata ggtttcacgt accagatgca tcaccggaga
    20221 attagcgtcc ggctgccagc caggatatgc gcctttcgct tcggttttcg cgccagccag
    20281 tttacccagc gaatccagca tgctcaccac gtagtcttta ccgctgtcga ttagtgaacg
    20341 gatcaggcag tgaatttcta cgttattgtc agtcatggtc accacaccga cgttcaggga
    20401 ggtttcgacc acaccttttg ccacgtcgga attgcggatc acaccgttcg gggtggcgtt
    20461 cagcagacga ataaaggtat cgcgagattt cgcaatcagg gcagctttat cgttcgctac
    20521 agagtccagc aacaaggcca gatttttctc tttctctgcc agctcgtttt tcaggatctc
    20581 ctgataggta ttcaccagag atttcagtgc gtcgacttta tcagctgcga cagcaatggt
    20641 cgcaaaggct tcacgcggga tggcgttacg cagtgtgccg ccgttgaaat cgataaggcg
    20701 caggtccagt tcttctgcat gacccgccag gaagcgcacc agcagtttgt tggcattacc
    20761 caggccaacg tggatttcac cgccggagtg accgcctttc agacctttta aggttaactt
    20821 gaaggtttca aaaccagctg gaaccgcttc acgatctaaa tgcaggttgg aggtgaagtc
    20881 gatacccccc gcacaaccca tgtagatttc accttcttct tcggagtcgg tgttaatcag
    20941 aatatcagcc tgcaaccagt tgctctgtaa gccgaacgca ccgtccatac cggcttcttc
    21001 ggtcatggtc agcagcactt ccagcgggcc gtgaaccacg ttttcgtcag ccagaaccgc
    21061 cagtgcagag gccataccaa tgccgttatc cgcacccagc gtggtgccgc gcgctttaac
    21121 ccattcgcca tcaatataag gctggatagg atctttcgtg aagtcatgca cggtgtcgtt
    21181 atttttctgc ggcaccatat cgaggtgggc ctgtaagacg accggtttac gattttccat
    21241 acctgcggta gcaggttgac gaatcaggat attacctacc tgatcgcgtt cgacatggaa
    21301 acctttctct tttgcccaac caacaatgta ttcagcgagt tgctcttcat gataggacgg
    21361 gtgaggaata gaacagattt tggcaaaaat atcccacagc ggctgtggag ataattgaga
    21421 cagttcagac acgttaagtc tccttgtcga tcacccgcaa aacagtattg caggtcacag
    21481 ggttagcaga aaatgttgtc aacacaagac aggcttgcga gatatgtatt gagaatacca
    21541 ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg
    21601 gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt
    21661 taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg
    21721 gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg
    21781 aaaggcatta ttgccgtaag ccgtggcggt ctggtaccgg gggcgttact ggcgcgtgaa
    21841 ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc
    21901 gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac
    21961 ctggtggata ccggtggtac tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt
    22021 gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc
    22081 ccgcaagata cctggatcga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc
    22141 tccggtcgct aatcttttca acgcctggca ttgccgggcg ttgttctttt taacttcagg
    22201 caggttacaa tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct
    22261 gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg
    22321 ctttaatcac ggcgcacaac cgcctgtgca gtcggctctt gatggtaaaa ccatccctca
    22381 ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat
    22441 cctcgacgtc caggcacgta ttgtgatgag cgatgctgaa cgtaccgacg atgatttata
    22501 cgataccgtg attggctacc gtggtggcaa ctggatttat gagtgggcca cccaggcgat
    22561 ggtgtggcaa caaaaagcct gtacggaaga agatccgcaa ctcagtggtc gacactggct
    22621 gcatgcggct acgttgtaca acattgccgc ctatcctcat ctgaaaggag atgacctggc
    22681 cgagcaagcg caggctttat caaaccgcgc ctatgaagag gccgcccagc gtctgccggg
    22741 cacgatgcgg cagatggagt ttaccgtacc cggcggtgcg cccatcaccg gctttttgca
    22801 tatgccgaaa ggcgatggcc cgttcccgac agtattaatg tgtggtggtc tggatgcgat
    22861 gcagacggac tattacagcc tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct
    22921 gactattgat atgccgtcgg tgggcttttc ttcaaaatgg aagctcaccc aggactccag
    22981 cctgttgcat cagcacgtct taaaggcgct gcctaacgta ccgtgggtgg atcacactcg
    23041 cgtcgcggcc tttggtttcc gtttcggcgc taacgttgcc gtgcgtctgg cataccttga
    23101 atcgccgcgt ctgaaagcgg ttgcctgtct tggtccggta gttcataccc tgttgagtga
    23161 ttttaagtgc cagcaacagg tgccggaaat gtatcttgac gttctggcga gtcgtttggg
    23221 gatgcatgat gcttccgacg aagcgttacg cgtggagctg aatcgctatt cattaaaagt
    23281 gcaaggattg ctggggcgtc gctgcccaac gccaatgtta tcaggctact ggaagaacga
    23341 tccgttcagc ccggaagagg actcacgctt aatcacctca tcatctgctg acggtaaatt
    23401 attagagatc ccatttaacc cggtgtatcg gaattttgac aaaggtcttc aggaaatcac
    23461 cggctggatc gaaaaacgct tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac
    23521 agttgcatca caacaggaga tagcaatgac gttaccgagt ggacacccga agagcagatt
    23581 gatcaaaaaa tttaccgcac taggcccgta tattcgtgaa ggtaagtgcg aagataatcg
    23641 attctttttc gattgtctgg ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga
    23701 attctggggc tggtggatgg agctggaagc gcaggaatcc cgttttacct acagctacca
    23761 gtttggtctg ttcgataaag caggcgactg gaagagtgtt ccggtaaaag atactgaagt
    23821 ggttgaacga ctggagcaca ccctgcgtga gtttcacgag aagctgcgtg aactgctgac
    23881 gacgctgaat ctgaagctgg aaccggcgaa tgattttcgt gatgaaccgg tgaagttaac
    23941 ggcgtgagtg aaatgtgccg gatgcatcac atccggcaat attcattaaa actgatacgt
    24001 catcccaacc gcgacagtat catcattatt aatattcagc ttattatcgc tatccagttg
    24061 gttgatttta taatcaacaa acgctgacat atttttgttg aaataatacg tagcaccgac
    24121 gtcgatataa ttgaccagat cttcatcacc gatgccctca atatctttcc ctttcgataa
    24181 gacataaccc agcgatggac gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa
    24241 gttctgtgtc ttattggcaa agccgccagt tattggcgtc attttgcgtg tttcagaata
    24301 gaaagttgcc agataaatat tattggcatc gtatttcaga cctgttgccc atgcttctgc
    24361 acgcttgcct gtgccacggc tttgcaggtt ctgctcgttg gtgcgatctg agttggtata
    24421 ggccccacta atggcgaaat cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc
    24481 atcgccgttt tgctttttaa cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa
    24541 gttcaggcca tcgataacgc cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc
    24601 gcgtttggtc ataaagttgt cggtctgcgc cgaggaatcg ccaccaaatt ccgggaacat
    24661 atcggtccag gcttccacgt catacaacgc gcccaggtta cgaccataat cgaaagaacc
    24721 caaatcttta tatttcaacc cggcaaaagc gagacgcgtt ttttgctgtg cagtatcact
    24781 ctccgcttta tttccggcaa actccgcttc ccaccgacca taaccagtca gttgatcgtt
    24841 aatttgagtt tcacctttaa aaccaaaacg gatataactc tggtcgccat ctttactggc
    24901 gttatcactc atataatgca tggctttaac tttgccatag acatccagtt tattaccgtc
    24961 tttattatat atttctgcag cctgtacaga tgcagatgcc acaatgccca tcaccactaa
    25021 tgccagagtg ctctttttca ttttca
//
LOCUS       DAJTUI010000058        24171 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTUI010000058 DAJTUI010000000
VERSION     DAJTUI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.058"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(12..584)
                     /locus_tag="OO464_002499"
     CDS             complement(12..584)
                     /locus_tag="OO464_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR6227608.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            802..1662
                     /gene="tesB"
                     /locus_tag="OO464_002500"
     CDS             802..1662
                     /gene="tesB"
                     /locus_tag="OO464_002500"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR6227609.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1711..2997)
                     /gene="amtB"
                     /locus_tag="OO464_002501"
     CDS             complement(1711..2997)
                     /gene="amtB"
                     /locus_tag="OO464_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6227610.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3027..3365)
                     /gene="glnK"
                     /locus_tag="OO464_002502"
     CDS             complement(3027..3365)
                     /gene="glnK"
                     /locus_tag="OO464_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6227611.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3546..5327)
                     /locus_tag="OO464_002503"
     CDS             complement(3546..5327)
                     /locus_tag="OO464_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6227612.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5320..7092)
                     /locus_tag="OO464_002504"
     CDS             complement(5320..7092)
                     /locus_tag="OO464_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6227613.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7122..7580)
                     /gene="decR"
                     /locus_tag="OO464_002505"
     CDS             complement(7122..7580)
                     /gene="decR"
                     /locus_tag="OO464_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR6227614.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7734..8552)
                     /gene="cof"
                     /locus_tag="OO464_002506"
     CDS             complement(7734..8552)
                     /gene="cof"
                     /locus_tag="OO464_002506"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR6227615.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8652..10344
                     /locus_tag="OO464_002507"
                     /pseudo
     CDS             8652..10344
                     /locus_tag="OO464_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10409..11104
                     /gene="queC"
                     /locus_tag="OO464_002508"
     CDS             10409..11104
                     /gene="queC"
                     /locus_tag="OO464_002508"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6227616.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11156..11554)
                     /gene="fadM"
                     /locus_tag="OO464_002509"
     CDS             complement(11156..11554)
                     /gene="fadM"
                     /locus_tag="OO464_002509"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR6227617.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11648..12019)
                     /locus_tag="OO464_002510"
     CDS             complement(11648..12019)
                     /locus_tag="OO464_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR6227618.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12170..14041)
                     /gene="ppiD"
                     /locus_tag="OO464_002511"
     CDS             complement(12170..14041)
                     /gene="ppiD"
                     /locus_tag="OO464_002511"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6227619.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14233..14505)
                     /gene="hupB"
                     /locus_tag="OO464_002512"
     CDS             complement(14233..14505)
                     /gene="hupB"
                     /locus_tag="OO464_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6227620.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14714..17068)
                     /gene="lon"
                     /locus_tag="OO464_002513"
     CDS             complement(14714..17068)
                     /gene="lon"
                     /locus_tag="OO464_002513"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6227621.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17256..18530)
                     /gene="clpX"
                     /locus_tag="OO464_002514"
     CDS             complement(17256..18530)
                     /gene="clpX"
                     /locus_tag="OO464_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6227622.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18656..19279)
                     /gene="clpP"
                     /locus_tag="OO464_002515"
     CDS             complement(18656..19279)
                     /gene="clpP"
                     /locus_tag="OO464_002515"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6227623.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19526..20824)
                     /gene="tig"
                     /locus_tag="OO464_002516"
     CDS             complement(19526..20824)
                     /gene="tig"
                     /locus_tag="OO464_002516"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6227624.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21168..21485)
                     /gene="bolA"
                     /locus_tag="OO464_002517"
     CDS             complement(21168..21485)
                     /gene="bolA"
                     /locus_tag="OO464_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6227625.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21790..22368
                     /locus_tag="OO464_002518"
     CDS             21790..22368
                     /locus_tag="OO464_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6227626.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22412..23887
                     /gene="ampG"
                     /locus_tag="OO464_002519"
     CDS             22412..23887
                     /gene="ampG"
                     /locus_tag="OO464_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR6227627.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5664 a         6663 c         5820 g         6024 t
ORIGIN      
        1 gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt cacctgagtt
       61 ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg cacggcggtt
      121 tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat caccggctga
      181 acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact caacagaata
      241 cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa tggtgactgt
      301 ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc ggctaacgac
      361 gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt ctgacggatc
      421 cagacagtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc tgaaatagac
      481 gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca agccgccaac
      541 gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt ctccttatta
      601 tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa tagtaacgtc
      661 aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt cagactattt
      721 cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct tgctgcggca
      781 gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac attgttaaat
      841 ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg tttacgccag
      901 gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac cgtcccggaa
      961 gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag taagaaaccg
     1021 attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg ccgggttgct
     1081 gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc accagaagcg
     1141 ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct gccttcggaa
     1201 acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga taaattcatc
     1261 tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa aggtcacgtc
     1321 gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga tgacctgcgc
     1381 gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt agctctacag
     1441 ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga ccattccatg
     1501 tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga gagcacctcg
     1561 gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg cgtgctggtt
     1621 gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag ccggaggtga
     1681 aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct cgccgtggct
     1741 gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa ccgtcagatc
     1801 cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa tggcgatgct
     1861 ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc ccacgccgcc
     1921 cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac aaacgccgtg
     1981 cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca tggtaacgcc
     2041 ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa ccccaatgta
     2101 gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg ctcccagcag
     2161 agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg ccgccgccgt
     2221 tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc ccactgaccc
     2281 ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga ccatcggcag
     2341 gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca gatacgcgcc
     2401 caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga aatccagcgc
     2461 accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa tgtaagagag
     2521 cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt ccgccagcgc
     2581 cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta cgtggatata
     2641 ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca accagttaat
     2701 gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa ccacccagag
     2761 aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca cgtttttgcc
     2821 gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca gcaccagcgc
     2881 agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg cagccattac
     2941 cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta tcttcatttt
     3001 tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc gccggtacga
     3061 atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat ttttccggtg
     3121 taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc aatcgccaca
     3181 tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta cagctcggca
     3241 tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg aataccaatg
     3301 gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac ggtcaccagc
     3361 ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag aaggtattgc
     3421 aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa gattgaaaat
     3481 gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat gaaatattgc
     3541 gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc ttcgcccgca
     3601 agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg ctggtgagtg
     3661 ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc aataatagtt
     3721 gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc cagagcatgt
     3781 tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag gatcaggatt
     3841 tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc gactgagaga
     3901 ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg cgccagctcc
     3961 gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc ccgccccagc
     4021 gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac cattgccacg
     4081 ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag gtgaatctca
     4141 ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt accactgccg
     4201 gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat gttctttagc
     4261 accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt gccactctgt
     4321 aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc aaacacgcgc
     4381 tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc gattaagggt
     4441 tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc aatggtgccg
     4501 ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga aaacagactc
     4561 agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc catatagtgt
     4621 gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa ctgctggata
     4681 acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag ataggcgcgc
     4741 acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac cagcaccacc
     4801 gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat cgccaccagc
     4861 atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac gtagagatcg
     4921 cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg ttgggtatca
     4981 aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa ctgttgcact
     5041 gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc ggcggcaaac
     5101 agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg caggttattt
     5161 ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac ttctgccgcc
     5221 gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg cgaaccatac
     5281 gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga cggcctcctc
     5341 gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat acatatcgcg
     5401 ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga tatgtccgtg
     5461 ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg cactgatgat
     5521 taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct ctgtgcgtcc
     5581 gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta ataacgctcg
     5641 agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct cgcccacttc
     5701 tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta accgcgcgac
     5761 atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat tcgccacggt
     5821 gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc agctatcgag
     5881 ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga cgtcgaaatg
     5941 acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc aaatacccag
     6001 catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt cagtctgcgg
     6061 atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca cgggttcgct
     6121 accatcgttc accaccggcg cttccgccag catggcgcga atacggctgt atgcagcact
     6181 accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa tcatcagacc
     6241 taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca ccaccatcca
     6301 actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga tggtcgggtc
     6361 gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt cggcatccgc
     6421 ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc ggatactggt
     6481 gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca gcttaaagcg
     6541 ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca tcggtaacag
     6601 ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca cagcgcagcc
     6661 cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca cgacgcgatc
     6721 gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt aaaactccgg
     6781 gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca gttggtaaga
     6841 cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa cggcaatcag
     6901 caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt ctgtcacgcc
     6961 atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga taatgacaag
     7021 caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc ttaattgagc
     7081 aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat gggtaaagaa
     7141 gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag ccccggcacg
     7201 ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac ctggacgcgc
     7261 atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg catttcggta
     7321 accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt tatcagcaca
     7381 aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt gccgataagg
     7441 ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt cagattaacg
     7501 gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc cagcagctta
     7561 cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt taattctatt
     7621 ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc acaaaatgac
     7681 aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc tcgttattcg
     7741 ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga gacagcctga
     7801 tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg cggcatcgca
     7861 ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt catcgcatca
     7921 ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac cgtcaatgca
     7981 gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga aaaacacaaa
     8041 tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag atcgtcgtga
     8101 tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac atcgattatc
     8161 tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc tttcccggta
     8221 aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg ctgatacagc
     8281 accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc ttccagagaa
     8341 tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc cccgagaata
     8401 tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc gcgttcacgc
     8461 agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat caataaagtg
     8521 ccatccatat caaatgctac cagacgagcc atttctttct ccactctgtg acacggttaa
     8581 tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa ataatattgt
     8641 tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg tggcaacctt
     8701 cagccgcaaa ccgtcaccgt cagcgaactg gccgaacgct gtttttgcag cgaacgccat
     8761 gttcgtacgc tgttgcgtca ggcacaggag gcgggatggc tggagtggca ggcgcagtca
     8821 ggacgcggaa agcgcggaca attacgcttt ctggtcacgc cggaatcgct acgcaatgcg
     8881 atgatggaac aggcactgga aaccggaaag cagcaagatg tgctggagct ggcgcaactg
     8941 gccccaggtg agctgcgcac tctgttacag ccatttatgg gcggacaatg gcaaaacgat
     9001 acacccacgt tgcgtattcc ctactatcgc ccgctcgaac cgctacaacc aggctttttg
     9061 cccggccgtg ccgagcagca tctcgccggg cagatatttt ccggcctgac ccgcttcgat
     9121 aataatactc agcgcccgat tggcgattta gcgcatcact gggaaacctc tactgacagg
     9181 ttacgctggg acttttatct tcgttcaacc ctacactggc ataacggcga tgcagtaaaa
     9241 gcctcacact tacaccagcg attattgatg ctgttacaac tgccagcact ggatcaatta
     9301 tttattagcg tgaagcgtat tgaagtcacc catccgcagt gtctgacctt ctttttacat
     9361 cgccccgatt actggcttgc gcaccggctg gcgagctatt gcagccatct ggcgcatccg
     9421 caattcccac tgatcggcac tggtcctttt cgcttaacac aattcacagc ggagctggtg
     9481 cgcctggaga gccatgatta ttaccatttg cgtcatccgc tgcttaaagc ggttgagtac
     9541 tggataactc cgccgctttt cgaaaaagat ttgggaacca gttgtcggca tcccgtgcaa
     9601 atcaccatcg gcaaaccgga ggagctgcaa cgggtcagcc aagtcagtag tggcatcagt
     9661 ttaggttttt gctatttaac gttgcgcaaa agtccccgac tctccctctg gcaggcgcga
     9721 aaagtgatct ccattattca tcaatccggt ttattacaaa cgttagaagt cggagaaaac
     9781 ctgatcaccg ccagtcatgc attactgcca ggctggacta ttccgcactg gcaagtaccg
     9841 gatgaagtca aactaccgaa aaccttgacg ctggtttatc acctaccgat agaacttcat
     9901 accatggcag aacgcctaca ggcgacactg gcagcggaag gctgtgaact cacaattatt
     9961 tttcataacg caaaaaactg ggacgacacg accctactgg cacacgcaga cctcatgatg
    10021 ggcgacagat taattggcga agcaccggaa tatactctgg agcaatggct acgttgcgat
    10081 ccactatggc cacatgtttt cgacgctcca gcatatgcac atctgcaatc gacgctggac
    10141 gcggtgcaag taatgcctga tgaggaaaac cgatttaatg ccctgaaagc ggtttttagc
    10201 cagttaatgg cagacgcgac gctgacgccg ctgttcaact atcactatcg cattagtgcc
    10261 cctcccggcg tgaacggtgt gcgactgaca ccgcgcggct ggtttgaatt taccgaagcc
    10321 tggcttcccg cgccgtcgca atgaatagct ggtccgggtt aatcgcaggc gttaccataa
    10381 cgcttttatt gttcaagcag gattatctat gaaacgtgct gtcgttgtgt tcagtggagg
    10441 ccaggattcc accacctgtc tggtgcaggc attacaacaa tatgatgaag tccattgcgt
    10501 gacgttcgat tacggtcagc gacatcgcgc agaaatcgac gtggcacgcg aactggcgct
    10561 gaaactggga gcacgcgcgc ataaggttct tgatgtaacg ctgctcaacg agctggcggt
    10621 cagcagcctg acacgtgaca gcattccggt gcctgattat gaacctgaag ccgatggcat
    10681 cccgaatacg tttgtcccag ggcgtaatat tttgttcctg acgctggcgg caatatatgc
    10741 gtatcaggta aaagcagaag ccgtaattac cggcgtctgt gaaacggatt tctccggcta
    10801 cccggattgc cgcgatgagt ttgtgaaagc actaaaccat gccgtcagtt tgggcatggc
    10861 gaaagatatt cgttttgaaa cgccgctgat gtggattgat aaagcggaaa cctgggcgct
    10921 ggcagattat tacggcaaac tggatttagt ccgtaacgaa acgttgacct gctataacgg
    10981 cattaaaggc gacggttgcg gtcattgtgc ggcatgtaat ttacgcgcca acggtttgaa
    11041 tcattatctg gccgataaac cgacggtgat ggcagcgatg aagcagaaaa ccgggttgag
    11101 gtaattattc cgggtgtcgc cggatgcggc ttgagcatcc ggcaccacaa aacgtttact
    11161 taaccatctg ctccagcttt tcgcgcaatt ccccttccag agctaatgct ttctgcgttt
    11221 taagatcaat acaaacaaac gtaataagcg catccgctac cacctgccct tccggctcca
    11281 gtgtaatgac ctggtttaag atgccgcttt taccgtttaa ttgctgcaac tgactggtaa
    11341 tagttaacag gtcgcttaat accgctggac gacgatagtt aatattgata ttgaccacga
    11401 cgaaggcgat gttatgggcc gtcatccact gaaaactgtc gctattttcc accccatccc
    11461 agcgggcttc ttcgagaaat tcaaggtagc gggcgttgtt gacgtgctgg taaacgtcga
    11521 gatgatatcc acgaactttg atttgtgttt gcatagcgca ataaccttac gttgttgtta
    11581 taagtacaga ggtcataact ggtatgacct cttcagtctg gcaaaaaatt gccactatgc
    11641 aaattaatta cagggttaat accgccagat tacgttccac cagcgaattg cccatccccg
    11701 gcacctgctt tagatcctcc accgttttaa acggaccgta ctcttcgcga taactgacaa
    11761 tcgcctgcgc tttcttcagg ccaacgccat tcatcgcgcg ggctagctct tccgcgctgg
    11821 cattattaat gctgacccgg gtgccttctt cgtcactggc tttcgccggt actgctgctt
    11881 tactttgtgc tgcaggagct tccgctttgg tttctaccgc cgtcggtttc gccacagaag
    11941 ctgccgccag cgcgctatga gacattccgg cacaggccag ggacagtgta atgagcagtg
    12001 ctttaattcc gtgtttcata ctgttttctc cttgtttgtt aacagtggaa tcaccttaac
    12061 gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg cgaaagcgac
    12121 cttttcttgt tacagctgca acgcattttt ttgcgaggcg gcttcaggat tattgctgtt
    12181 ccagcgcatc gccaattttg attttcgcct ctttacgcag gttactcatc agagcttcaa
    12241 agacgatttg tgcgttgttc tgggtgatac cctgcaccat cgcttttttc tgatcttccg
    12301 gcattgaacc ttgtttcact tcatccagcg ccagcagaac cacattacct tgcatatcgg
    12361 tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac gccgcctggc
    12421 taatcgggtc acgaccggaa cggcttaagg ttttcggctc gccaaatttc agaccggcag
    12481 cctgcatagc ttccgcacct ttgccggctt tcaaatcaac cagcagtttc tcagcatcca
    12541 ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc cttaacttgt tcctgaacat
    12601 ctgccaacgg tttcaccgct tccggtttgt gctcgctgac gcgcagcacg aatgcgcggt
    12661 cgccgtctac ggtgatgatg tcagagttga tgcccgacgc gccgttttca cctaccagac
    12721 cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa ctcttccggc aggttatctt
    12781 tgctgaacca acccgtctga gtggctttaa cgccggcagc ttgctctgca ccggccagag
    12841 actcggtgtc gttgcttgcc gcatcgctca ctttctgctg cagcgcatag tacgcatcga
    12901 gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg tacttcgtct aacgatttca
    12961 ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa accgaccgaa gatttgatga
    13021 caccagacag ttggcctttt tctttcagac cagcattttt cagttcgtcg gggatagtgg
    13081 catcttctaa ccaacccata tcgccgccgt tacgggcaga gataatatcg gcagattttt
    13141 ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc atcaagtacc gctttcgctt
    13201 catcttcagt tttggtctgg atgatgctgt agcgggtacg ctgcggctgg gtgaattgat
    13261 cctgatgctg atcgtagtag ctctggatat ccgcatcgct aaccggttgc tgcatcgttg
    13321 cggcaaccag cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa ttgtttttgt
    13381 tttgttcgta gtagctggca atctcctgtt cggtcacagg ctgcttcgcc gccagcgcgt
    13441 taacatcgat agtcgcctca cgcaccacgc gttgttgcgc gaccagtgcc gccagctcgt
    13501 cggtttcacc tttcagcata aaatcggtac cggcaacgcc gttaatcagc tgttgggtag
    13561 tgagctggtt acgcagcgcc tgggcgtact gatcggcggt catccccatc tggttgagga
    13621 taccgttata gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg gtcgcgaaaa
    13681 tcgcctgttt aacctgctca tcactgatac ccagtttcag ctcacgagcg tactgatcca
    13741 acagcgcctc gtcgatcaga cgattcagca cctgttgacg cagggttttc atatagcctt
    13801 cgttcgctgc cagctcggag tattgatcgc ccagctgttg ctgcatacga ttacgctcgc
    13861 tgttaaaggc gttctcgaac tgcccacggc tgatttcctg gtcattcact tttgcggcgt
    13921 agttattgcc tccgccaatc aggtaaccac tcacgccggt caatatgaac gacacgataa
    13981 tgataccgaa aataatcttg agcacgagac tgtttgcagc cgtgcgtaag ctgtccatca
    14041 tggtgtaaca acactccgct gtagatgact gtgtacctca cgcaacgtat cgatgcgcgc
    14101 aaaggcctat tgtgacaaga aacgggggca attgtcagcc cacaagacac agaaagtcac
    14161 cgaatacaaa taaaaaaggc acatcagtag atgcgccctt gaacttcgtc acatccccac
    14221 tggggacaac gcttagttta ccgcgtcttt cagtgcttta cctgcacgga agctcggtac
    14281 tttagcggca gcgatggtga tctctttacc ggtctgcggg ttgcggccag tacgggcagc
    14341 acgctcttta acggcaaaag taccaaaacc taccagtgct acatcatccc cttctttcag
    14401 agattcagtt acggaagcaa taatagcatc taacgcacgg ccagccgcag ctttagagat
    14461 atcagcccct gcagcaatct tgtcgatcaa ttgagattta ttcactcttc tcttcctctt
    14521 tataatttat atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt atatcaggcc
    14581 agccatcccc ttacaacacc ccttaataag ggcaagcccg atccgctatc taacttagcg
    14641 agacaaaaaa aggctggcaa gcccgaatta gcctgccagc cctgttttta ttagtgcatt
    14701 ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc agacggttca ttttgcagcg
    14761 ccagagtcag aacttcctca atgcgcttca caggatgaat gtccagatcg gcaattacgt
    14821 tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg aattagcact gttttaatcc
    14881 cgccgcgatg cgctgccagg agtttttctt tcaaaccacc gatcggcagt acctgaccac
    14941 gcagagtgat ctcaccggtc attgccacat cggcacgaac cgggttaccg gtcaggcaag
    15001 aaaccagcgc ggtgcacata gcaataccgg cactcggacc atctttcggc gtcgcacctt
    15061 ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc agggttgatc cccagttttt
    15121 ccgcacgcgc acgaaccacc gttaacgccg cctgaataga ctcctgcatc acttcgccga
    15181 gcgaaccggt ataggtcagt ttgcctttac ccggaacaca tgcggtttca atggtcagca
    15241 agtcaccgcc cacttccgtc cacgccagac cgattacctg accgacacgg ttttcgttat
    15301 cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc atgcaggtta tcgccgttaa
    15361 tttcgatatg ttttaatgac ttatcgagca gtaactgctt aaccgctttg cggcacagtt
    15421 tggagatttc acgctccaga ccacgcacgc ccgcctcacg ggtgtagtaa cgaataatgc
    15481 cgataatggc gctatcgtcg acggtcagct cacctttttt cagtgcatta cgttcaatct
    15541 gcttcggcag caggtgacgt ttggcgatgt tcagtttttc atcttcggta taaccggaga
    15601 ggcgaatcac ttccatacga tccagcagcg gtgccggaat gttcatggag ttcgacgtcg
    15661 cgacaaacat cacgtcgctg agatcgtaat ccacttccag gtagtggtcg ctgaacgcta
    15721 cgttctgctc tggatccagc acttcaagca gtgcagaggc cggatcgcct cgcatgtcag
    15781 aagacatttt gtcgatctca tcgagcagga acagcggatt tttcacgccc actttcgcca
    15841 ttttctggat cagtttaccc ggcatagaac cgatgtaagt acggcggtga ccacggattt
    15901 ccgcttcatc acgcacgccg cccagcgcca tacggacata tttacgcccg gtggctttgg
    15961 caatggactg accaagagag gttttaccta cccccggcgg ccctaccagg cagaggatcg
    16021 gtcccttgat tttgttgaca cggctttgaa ccgcaaggta ctcaaggatg cgatctttca
    16081 cgcgctccag accataatgg tcggtatcaa ggatttcctg cgcctgacgc aggtcttttt
    16141 tgaccttgct gcgcgcattc cacggtacct gtaccatcca gtcgatataa ccacgcacta
    16201 cggtcgcttc tgccgacatc ggagacatca ttttcagctt ctgcaactct gcttccgctt
    16261 tctcttttgc ctctttcggc atcttcgccg cgtcgatttt gcgcttcagg gcttcgtttt
    16321 cgtccggcac gtcgtccatt tcaccgagtt ctttctgaat agctttcatt tgctcgttca
    16381 gatagtactc acgctgggat ttctccatct gctttttaac gcggttgcga atgcgtttct
    16441 caacctgcag cagatcgatt tccgattcca tcattgccat cagatattcc agacgttcgt
    16501 taacgtcgga catctccaga acggactgtt tgtcagccag tttcagcggc atatgtgcag
    16561 caatggtatc cgccagacgc gccggatcgt cgatgctatt cagcgacgtc agcacttctg
    16621 gtgggatttt tttgttcagc ttgatgtagc cttcgaactg gctgattgca gtacgcacca
    16681 gcacttcctg ttcccgctca tcaatggtcg gcgactccag atactccgcc ttcgcagaaa
    16741 agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc tcgaccagca
    16801 ctttgacggt gccgtcaggc agtttcagca tctgcaatat agaggccacg gtcccgacgg
    16861 tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg accagcataa
    16921 tttttttatc atggtccatc gccgcttcca gacaacggat agatttttcc cgcccgacaa
    16981 ataaggggat gaccatgtgc ggataaacca ccacatcgcg cagcggcaat acggggattt
    17041 caatgcgttc agaacgctca ggattcatag agctctctct tagtttaatt tccgccaggt
    17101 aatcagatga cacgactgtg cttcacgcca tctattaaca tgtacgtcag tatatgggga
    17161 tgtttccccc acattcaacg ccgagaatag aggaaaaatt aaaggggaga taaaatcccc
    17221 cctttttggt taactaattg tatgggaatg gttaattatt caccagatgc ctgttgcgct
    17281 tccggcttac cataaatcag caacggtttg ctttgaccat caattaccga ctcgtcgata
    17341 accacttttt cgacgtcttc catggacggc agatcgtaca tggtatcgag cagtgcggct
    17401 tctacgatgg aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt cttagcgata
    17461 gcatccagcg cctcgtcacg gaattccaga tccacgcctt ccagattaaa cagcgcctga
    17521 tactgcttgg tcagggcgtt tttcggctct ttgaggatct gaatcagagc ttcttcgctc
    17581 agttcattca acgttgctac aaccggcaga cgaccaatga actcagggat aagaccaaac
    17641 ttgatcagat cttccggttc aacctgcgcc agcagctcgc cttcgcttgc tttgtcggac
    17701 ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg gtgggaaatc
    17761 actttatcca gaccggcaaa cgcaccgcca caaataaaca ggatcttaga ggtatcaacc
    17821 tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg gtggaacagc agctaccgta
    17881 ccttcgatca gtttcaacag tgcctgctgt actccttcac cggaaacgtc tcgggtaatg
    17941 gacgggttgt ctgacttacg agaaatcttg tcgatttcat cgatgtagac aatcccgcgc
    18001 tgcgctttct gtacgtcgta atcgcacttt tgcaacagct tctgaatgat gttttcaacg
    18061 tcttcgccca cataaccggc ttcggtcagc gtggttgcgt cggccatggt gaacgggacg
    18121 tccagcaggc gcgccagcgt ttcagccagc agcgttttac cggaaccggt cggaccgatc
    18181 agcagaatgt tacttttgcc caactcgaca ccattgctgg tatcgccgtt gcgcagacgt
    18241 ttgtaatggt tgtataccgc gaccgccagc acttttttcg cctgttcctg gccgataacg
    18301 taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta gcgcactgcg ttcacgatgc
    18361 ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac acattcgtcg
    18421 cagatataca cggatggacc ggcaatcagc ttgcgcactt catgctggct tttgccgcaa
    18481 aaagagcaat acagcaattt gcctgagcca tctttgcgtt tatctgtcat gggtcaaaac
    18541 ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg ccattctcag gcgcaagccg
    18601 cttacagcgt tgtgccgccc tagataagta tagcggcaca gttgcgcctc tggcatcaat
    18661 tacgatgggt cagaatcgaa tcgaccagac cgtattccac cgcttcaggg gcggaaagga
    18721 agcgatcgcg ctcggtatca cgttcaatct gttctaatga ttgacccgta tgaagcgcca
    18781 taagttcatt catgcgccct ttaactttca gaatttcacg ggcatgaatt tcgatatcgg
    18841 tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat cacgcgcgaa ttcggcaggc
    18901 aaaaacgttt accttttgcc cctgcggtca acaagaaagc gcccatcgag gccgcctggc
    18961 ccatacagat ggtgctgaca tcaggcttga taaactgcat ggtgtcatag atagacatcc
    19021 cggcagtgat caccccgcct ggggagttaa tgtacagata gatatctttt tctgggtttt
    19081 ccgcttccag gaacagcatc tgcgccacaa tcaggttagc catgtggtct tcaacctggc
    19141 cagtcagaaa aatgacgcgt tccttaagta gacgagaata gatatcaaaa gagcgctcac
    19201 cgcgtgaggt ctgttcaatg accatcggca ccagcgccat atggggtgca aagttatctc
    19261 gttcgccgct gtatgacatt tccgtctcct ggataaaatt gaaaaaaacc tgctgtaccg
    19321 attgtaacct tatggacgga ttatcagcta gtccctgtgt tatgggtacg gcatcaccat
    19381 atttcaagca taacaatctt ttgttgttac gctaacaccg aaagaggctt ttcgcacggc
    19441 tccatgcaaa attcatgaca aaaaaaagcc caccacctgg cggtgacggg cctttgtgcg
    19501 aatttagcgc gttatgctgc gtaaattacg cctgctggtt catcagctcg ttgaaagtgg
    19561 tttctttttc agtcactttc gctttcgcca gtacagcttc aacagcctgt tcttccagag
    19621 caacattgcg catgttgtcc atcagttctt tgtttttgct gtagaactcg ataacttctt
    19681 tcggatcttc gtacgcagaa gccatctctt cgatcaggcc tttcacgcgc tcttcgtcag
    19741 ctttcagctc gttggtgcgg ataacttcgc ccagcagcag gccaacaact acgcggcgtt
    19801 tagcctgttc ttcgaacagt tcgcgcggca gttccagagc ttgtttttcg ttgccaccga
    19861 aacgctgtgc agcctggcga cgcagaacgt cgatttcgct gtcgatcagc gcagccggta
    19921 cgtcgatgtc gttagctttt accagacctt cgatcgcctg agacttaacg cggttacgga
    19981 tggcgctctt cagctcgcgc tccatgtttt tacgcacttc agcgcgcaga ccttctacgg
    20041 aaccatcttc aacgccgaaa cgtttgatga attctgcagt cagttccggc agttcacgct
    20101 cttcaacttt cttcaggttg atagcgaatt tcgctgcttt acctttcagg ttttctgcgt
    20161 ggtattcttc cgggaaggtc acgtcgatgg tgaactcttc gccagctttg tggcctttga
    20221 taccgtcttc aaagcccggg atcatacgac cctggcccat cgccagtacg aaatcagacg
    20281 ctttaccgcc ttcgaactct tcgccgtcta cagaaccggt gaagtcgatg gttacgcggt
    20341 cttctgcttc aacagcgccg tctttttctt tccaggtcgc ctgctgttta cgcagagtat
    20401 ccagcatgcc gtcaacgtca gcgtcggtca cttcaacgat cggtttttca acttcgatcg
    20461 cttccagacc ctgcagttca acttccggat aaacttcaaa ctctacagag taagtgaagt
    20521 cttcacccag cttgtattcg cccggaacat aagtcggtgc gccagccgga ttgatttttt
    20581 ctttaatgat ggcgtcaatg aagttacggc tcatcaggtc acccagaacg tcctggcgta
    20641 cagacgcgcc ataacgctga gcaacgatat tcattggcac tttgcctttg cggaagccgt
    20701 caatacgtac ttttttcgca acgttgacca gctcgctttt aacagcggtc tcgatgctgt
    20761 cagcagcgat agtaatcgtt acacggcggc caaggccttg agtggtttca actgaaactt
    20821 gcatcttgtt acctcaaaaa atcacagtgc tcggtcaact cttaccccgc aagcacaggt
    20881 tgttggcttc gcggaaccgg gatgttctta taatcaatca cattccctgt taccagaatc
    20941 atcccgaaag cattcaaata ggacgcggca ttatagcggc atcactttta tgagtcgaga
    21001 acggttattg cgcgctgtcg cgtatttttt cacaattccc gggcaatttt cgtctgaatg
    21061 ctgggcaaaa agcgcaaaat ccagacaaaa caaaacggcc cgcaggccgt ttttcataaa
    21121 atctttagca acatactgga aaagcgccga cagttgcaaa tgcgttttta cgcgatgctt
    21181 cctgctccac gacagggagg agaggcaaag acggtgtcct gcaacccttc ccactcctta
    21241 atagtgtaag tatgcagagc cagcgcatga acggtagtag agagttcctc cgctaaagta
    21301 ctgtaaatca ttcgatgacg attcagaaaa cgttcacccg taaaacgatc gctgaccagc
    21361 acaactttaa aatggctttc agagccggct gggacattgt gacgatagct ttcatccact
    21421 acttcgagga atacgggttg gaacgccgcc cttaattttt cttctatccg ctcacgtatc
    21481 atcatgaaat atctcctccg acaacgctga gatgtcatcc atccctttaa atactagccg
    21541 cttttaccgt ttccattgca gcttaacaac aaatatttag gtttcgtcct ggtttttccg
    21601 tcaagcgtat gaagtttact gatttaaaga ttgaaacaca ggcctcgcat cagcgtttct
    21661 tttaccaggg gtgattattt cgtcaacatg atgaatttac atgccttacc cacttcccct
    21721 cgccgttggc aatgttatga tggcgggaat ttttaccaaa cacgcttaag agtctctgag
    21781 agcccgaaca tgttcaaaaa aatcctcttc ccgttagttg ctctgtttat gcttgcagga
    21841 tgcgcaaaac cgccaacaac tattgaagtt tccccgacga ttacactgcc acagcaggat
    21901 ccaagcctga tgggcgtcac cgtaagcatt aatggtgccg atcagcgtac cgatcaggcg
    21961 ctggcaaaag tcacccgcga taatcaaatc gttaccctga ccgcctcccg cgctctgcgt
    22021 ttcctgctgc aagaagtgct ggaaaaacag atgaccgcgc gtggttacat ggttggtccg
    22081 aatggcccgg ttaatctgca aatcatcgtt agccaactgt atgctgacgt gtcccagggc
    22141 aacgtgcgct acaacatcgc taccaaagcg gatatcgcca tcatcgctac cgcgcagaat
    22201 ggcaacaaaa tgaccaaaaa ctatcgtgcc agctacaacg ttgaaggtgc gttccaggcc
    22261 tccaacaaaa atatcgccga tgcggttaac agtgtgctga ccgataccat cgctgatatg
    22321 tctcaggaca ccagcattca cgaattcatc aagcagaacg cgcgttaatt tctgccctct
    22381 ggcccggtgc aagccgggcc tgtagacgcc catgtccagt caatatttac gtatttttca
    22441 acagccgcgt tcagccatat tgctgatcct gggttttgct tccgggctac cgctcgccct
    22501 gacatccggc accttacagg cctggatgac ggtcgaaaat atcgatctca aaaccattgg
    22561 tttcttctct ctggtaggcc aggcttacgt ttttaaattc ctctggtcac cgctgatgga
    22621 ccgctacacg cccccatttt ttgggcggcg gcgcggttgg ctgctcgcca cgcaaatcct
    22681 gttattagtc gccattgcgg cgatgggttt tctcgaacca ggcacccaac tccgctggat
    22741 ggcggcgctg gcggtggtga tcgctttttg ctctgcctcc caggatattg tcttcgatgc
    22801 gtggaaaacc gatgtgctcc cggcagaaga acgtggtgcg ggcgcggcaa tcagcgtgct
    22861 gggttaccgt ttagggatgc tggtttccgg cggcctagcc ctgtggctgg cagataaatg
    22921 gttgggctgg cagggcatgt actggttgat ggcggcgctg ttgatcccct gtattatcgc
    22981 gacgttgctt gcaccagaac caaccgacac cattcctgtg cccaaaacgc tggaacaagc
    23041 ggttgttgca cctctgcgag atttctttgg tcgcaataat gcctggctta ttttgcttct
    23101 tatcgtgctg tataagctgg gcgacgcatt cgccatgagc ctgacaacca cgtttttgat
    23161 tcgcggcgtc gggtttgatg cgggtgaagt aggcgtggta aacaaaacgc ttggcttact
    23221 ggcgaccatt gttggcgcat tgtacggtgg gattttgatg cagcgcctgt cgctgttccg
    23281 ggcgctgctg attttcggca ttttacaagg tgcgtctaac gctggttact ggctgctgtc
    23341 gattactgat aagcatctct acagcatggg cgcagccgtc tttttcgaaa acctctgtgg
    23401 cgggatgggc acatcagcct ttgtcgcgct gttaatgacg ctgtgtaata agtcattttc
    23461 cgctactcaa tttgccctgc tctcagcgct ttccgctgta gggcgagttt atgtcggccc
    23521 cgtggcgggt tggtttgttg aagcacacgg ctggtcgaca ttctatctat tctccgtcgc
    23581 cgctgccgta ccggggctta ttttgctgct ggtttgccgc cagacgcttg aatatacacg
    23641 agtaaatgac aactttatct cccgtaccga atatccggca ggttatgcct ttgccatgtg
    23701 gacactggcg gcgggcgtca gcctgttggc cgtgtggtta cttctgttga cgatggacgc
    23761 gctggatttg acgcacttct ctttcctgcc tgctctgctg gaagtcgggg ttttagtcgc
    23821 cctttctggc gtcgtgcttg gtggtttgct ggattatctg gcgctacgaa aaacgcatct
    23881 gacgtaatct gtaaatatta tttagctgta gtaatcactc gccgtaatta ttacggctaa
    23941 ataaacatca gcatcgctta ttaatatttg ttcttttgtg cggcttagcg tttggaaatt
    24001 attgacgcca tttataaatt ctatttttct tacacgattc agctaatgag tctttatttt
    24061 ctcaccaccc agttgtcact ttaatgataa ttatttgtta aataattgtt ttatttcgca
    24121 ttggttatac caattgccca ccaagactcc ttctgcactc cccttttgtt a
//
LOCUS       DAJTUI010000059        23920 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTUI010000059 DAJTUI010000000
VERSION     DAJTUI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23920
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.059"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(1..376)
                     /locus_tag="OO464_002520"
                     /pseudo
     CDS             complement(1..376)
                     /locus_tag="OO464_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            900..2828
                     /gene="thrS"
                     /locus_tag="OO464_002521"
     CDS             900..2828
                     /gene="thrS"
                     /locus_tag="OO464_002521"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6227628.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2832..3374
                     /gene="infC"
                     /locus_tag="OO464_002522"
     CDS             2832..3374
                     /gene="infC"
                     /locus_tag="OO464_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6227629.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3471..3668
                     /gene="rpmI"
                     /locus_tag="OO464_002523"
     CDS             3471..3668
                     /gene="rpmI"
                     /locus_tag="OO464_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6227630.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3721..4077
                     /gene="rplT"
                     /locus_tag="OO464_002524"
     CDS             3721..4077
                     /gene="rplT"
                     /locus_tag="OO464_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6227631.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4194..4318
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4200..4244
                     /gene="pheM"
                     /locus_tag="OO464_002525"
     CDS             4200..4244
                     /gene="pheM"
                     /locus_tag="OO464_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6227632.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4527..5510
                     /gene="pheS"
                     /locus_tag="OO464_002526"
     CDS             4527..5510
                     /gene="pheS"
                     /locus_tag="OO464_002526"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6227633.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5525..7912
                     /gene="pheT"
                     /locus_tag="OO464_002527"
     CDS             5525..7912
                     /gene="pheT"
                     /locus_tag="OO464_002527"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6227634.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7917..8216
                     /gene="ihfA"
                     /locus_tag="OO464_002528"
     CDS             7917..8216
                     /gene="ihfA"
                     /locus_tag="OO464_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6227635.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8317..9297
                     /gene="btuC"
                     /locus_tag="OO464_002529"
     CDS             8317..9297
                     /gene="btuC"
                     /locus_tag="OO464_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6227636.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9360..9911
                     /gene="btuE"
                     /locus_tag="OO464_002530"
     CDS             9360..9911
                     /gene="btuE"
                     /locus_tag="OO464_002530"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6227637.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9911..10660
                     /gene="btuD"
                     /locus_tag="OO464_002531"
     CDS             9911..10660
                     /gene="btuD"
                     /locus_tag="OO464_002531"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6227638.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10738..11202
                     /locus_tag="OO464_002532"
     CDS             10738..11202
                     /locus_tag="OO464_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6227639.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11449..12162
                     /gene="ydiV"
                     /locus_tag="OO464_002533"
     CDS             11449..12162
                     /gene="ydiV"
                     /locus_tag="OO464_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR6227640.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12226..13662
                     /locus_tag="OO464_002534"
     CDS             12226..13662
                     /locus_tag="OO464_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6227641.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13666..13857)
                     /gene="hemP"
                     /locus_tag="OO464_002535"
     CDS             complement(13666..13857)
                     /gene="hemP"
                     /locus_tag="OO464_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6227642.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13989..15035)
                     /gene="aroH"
                     /locus_tag="OO464_002536"
     CDS             complement(13989..15035)
                     /gene="aroH"
                     /locus_tag="OO464_002536"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6227643.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15192..16025)
                     /gene="ppsR"
                     /locus_tag="OO464_002537"
     CDS             complement(15192..16025)
                     /gene="ppsR"
                     /locus_tag="OO464_002537"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6227644.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16357..18735
                     /gene="ppsA"
                     /locus_tag="OO464_002538"
     CDS             16357..18735
                     /gene="ppsA"
                     /locus_tag="OO464_002538"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6227645.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18792..20438)
                     /gene="fadK"
                     /locus_tag="OO464_002539"
     CDS             complement(18792..20438)
                     /gene="fadK"
                     /locus_tag="OO464_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR6227646.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20495..20788)
                     /locus_tag="OO464_002540"
     CDS             complement(20495..20788)
                     /locus_tag="OO464_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6227647.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20785..22074)
                     /locus_tag="OO464_002541"
     CDS             complement(20785..22074)
                     /locus_tag="OO464_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6227648.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22130..23068)
                     /locus_tag="OO464_002542"
     CDS             complement(22130..23068)
                     /locus_tag="OO464_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR6227649.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23088..23852)
                     /locus_tag="OO464_002543"
     CDS             complement(23088..23852)
                     /locus_tag="OO464_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR6227650.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5908 a         5800 c         6300 g         5912 t
ORIGIN      
        1 tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg cgtggtttct
       61 agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt ttctgcatgg
      121 atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt ttgtgatatt
      181 ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa cttaagtcag
      241 aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct gatgaaggtg
      301 ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt ctgataatga
      361 tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg aggatgatga
      421 ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat tgtgtcactt
      481 ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa tactaccgcc
      541 aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg ctcgcagtaa
      601 acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct ggtgtactca
      661 ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct cccttttttt
      721 agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca tttgtgtacc
      781 tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac tgttaataaa
      841 caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa ggatataaaa
      901 tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct gtaagcccca
      961 tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta
     1021 atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg tcgatcatta
     1081 ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc
     1141 atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt attgacaacg
     1201 gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc gaagcactcg
     1261 agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag aaagtcagct
     1321 ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt tccattcttg
     1381 acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa tatgtcgata
     1441 tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa
     1501 cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt atttacggta
     1561 cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa gaagccgcga
     1621 aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc
     1681 cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg gaagtgtttg
     1741 ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc atgatggacc
     1801 gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg ttcaccacat
     1861 cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac gtacaaattt
     1921 tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag tttggtagct
     1981 gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc
     2041 aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt aacggatgta
     2101 tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc gtcaaactct
     2161 ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct gaggcggacc
     2221 tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt gaaggcgctt
     2281 tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca tggcagtgcg
     2341 gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag
     2401 acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg atggagcgtt
     2461 tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt gcgccagttc
     2521 aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa ttgacgcaaa
     2581 aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag attggcttta
     2641 aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt gataaagagg
     2701 tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga agcatggacg
     2761 taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt aaacaattgg
     2821 aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg
     2881 gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag cttggtattg
     2941 tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta gtcgagatca
     3001 gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc ctctatgaaa
     3061 agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta
     3121 aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc ctgattcgct
     3181 ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc
     3241 accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag
     3301 tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg ctcgctccta
     3361 agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc ttcacaggca
     3421 ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa atgccaaaaa
     3481 ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc
     3541 acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt aaacgtcacc
     3601 tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg tgcctgccgt
     3661 acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag gagagcacat
     3721 atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa
     3781 gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca ggctgttatc
     3841 aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg
     3901 tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag caaattcatc
     3961 aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga tatcgcagta
     4021 ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct ggcataagcc
     4081 agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa cattgacttt
     4141 tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa
     4201 tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag gaggctagcg
     4261 cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct ttttttgtat
     4321 gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac
     4381 acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga gcactgcccg
     4441 ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa cgaaccagtg
     4501 tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt gccagtgcga
     4561 aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt
     4621 tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg ccgccagaag
     4681 agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag caggcgctga
     4741 atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg gcggaaacga
     4801 ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg gttacccgta
     4861 ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca accgggccgg
     4921 aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac cacccggcgc
     4981 gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc cagacctctg
     5041 gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc gcgcctggcc
     5101 gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag atggaaggtc
     5161 tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac gacttcctgc
     5221 gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc ccgtttaccg
     5281 agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa gtgctgggct
     5341 gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa gtttactctg
     5401 gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc gtcaccgacc
     5461 tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata
     5521 gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga tcgatagcga
     5581 tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag aaccggttgc
     5641 cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc atccgaacgc
     5701 tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg
     5761 cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg gtgctgttct
     5821 gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg aagggatgct
     5881 gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg aactgcctgc
     5941 ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata acaccatcga
     6001 aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg cgcgtgacgt
     6061 tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg gtgcgactat
     6121 cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct atcttggccg
     6181 tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg
     6241 tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg tactgctcga
     6301 actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg
     6361 gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga agctgaatgc
     6421 tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca tctttggtgg
     6481 cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag
     6541 cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg cgtctcaccg
     6601 ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg cgacccgtct
     6661 gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca acgaagcaac
     6721 gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc tgataggcca
     6781 tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg aagtgaccga
     6841 aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg agattgaaga
     6901 agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg atgagccggt
     6961 acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa
     7021 aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg ttgatccgaa
     7081 agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc cgatctctgt
     7141 tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa
     7201 ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga
     7261 tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa
     7321 ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg atttgaaagg
     7381 cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt tccgtgcaga
     7441 agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag gtgaacgtat
     7501 tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta acggtcgcac
     7561 tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga
     7621 gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc
     7681 cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa
     7741 cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc tcgccataag
     7801 cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg ctaccgtcgc
     7861 caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt gaacctatgg
     7921 cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt agcaagcggg
     7981 atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg
     8041 aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat caacgcccgg
     8101 gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg gtgaccttca
     8161 gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac gagtaatctg
     8221 atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag agtcaccgta
     8281 aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc ccgccaacaa
     8341 cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct ggcgcttctc
     8401 ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac tcctcgtggc
     8461 gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg
     8521 gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct
     8581 ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt attgcttggg
     8641 caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc
     8701 actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt attgctggct
     8761 ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta cttttccacc
     8821 tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg cgtagactgg
     8881 cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc
     8941 aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg tttacccatg
     9001 tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt cagtgtggcg
     9061 ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg gttgtgtggt
     9121 ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag cgcattgctg
     9181 ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat tggcgtggtc
     9241 accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg acgttagccg
     9301 caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta ggagaaacga
     9361 tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg accacgctgg
     9421 agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt ggcttaacgc
     9481 cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt tttgtggtgc
     9541 tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa gagattaaaa
     9601 cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt gaagttaatg
     9661 gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc acagtcgcgc
     9721 cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg ctgtacccgg
     9781 atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa gtcatccagc
     9841 gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt aaactggcgt
     9901 tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta cccgcctggg
     9961 gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc cgaatggcgc
    10021 gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg gaagcattca
    10081 gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta
    10141 tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc tgacactgca
    10201 tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc tggctcttga
    10261 tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac gcgtacgtct
    10321 tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat tgctgcttct
    10381 tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca aaattctgag
    10441 cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca accacacgtt
    10501 gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca gtggacgcag
    10561 agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt ttcgccgtct
    10621 ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt atcccgaaaa
    10681 tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc gtacggaatg
    10741 cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca ccataaagca
    10801 ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt gaacgaccag
    10861 ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg cggtgtggac
    10921 tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct gccccgtgaa
    10981 accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct gcctggtgac
    11041 ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat ttacgatacc
    11101 aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc acttgataat
    11161 gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga aaaattgacg
    11221 gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca gacaaaaatt
    11281 ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc ctgaaaaagt
    11341 ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt atggcctcat
    11401 cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat gaagattttt
    11461 ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa ccagcaactc
    11521 cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt tcgtatcccg
    11581 acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt ttcagagcag
    11641 ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt tgcctggctt
    11701 aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc aggcgaacta
    11761 ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct caatgaagga
    11821 aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt aggcaatttg
    11881 ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg cgtaatgctg
    11941 gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt tattcgtgcg
    12001 atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat tgatacgcca
    12061 gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg cctgtggcct
    12121 gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg tttgctggtg
    12181 tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac cctgtctttt
    12241 attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc tacgccttta
    12301 aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag tattccatcg
    12361 tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc tggtatgtca
    12421 ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca actgggtgat
    12481 gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat ggactggcat
    12541 ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc ggttttacgt
    12601 tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat tccgacgacc
    12661 cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt ggagccaggc
    12721 gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga acatttttac
    12781 tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg tcattactgg
    12841 tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt tgtcgcacgt
    12901 accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt gatgaatacc
    12961 gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt tcttgatgat
    13021 tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag ctttgataat
    13081 caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc accatttgtt
    13141 gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt gacgcattat
    13201 ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga taacgcgcta
    13261 ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg cacattccgc
    13321 atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga gtttattgat
    13381 cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca agacgaggtt
    13441 agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt attgcgcaac
    13501 tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga attgcaccgc
    13561 ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt cagtcgtcca
    13621 cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact tagttaacag
    13681 cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat cgataatcag
    13741 tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct gcggggaagc
    13801 ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat aacgcataaa
    13861 aattaataat aattgatata acaatgattc ctattatcaa tattatggca gaccagagca
    13921 agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca tcaggaatgg
    13981 gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag gcgttcggta
    14041 tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg ctgaccgccg
    14101 acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc aatctccgta
    14161 gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg acggtgctgc
    14221 ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc aaactcgtgc
    14281 agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt gccgccacgc
    14341 ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg accatttttg
    14401 tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac ggcaatccgc
    14461 gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga agccatttcg
    14521 cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat taaatcagca
    14581 ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc cagctcattt
    14641 acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata gctgccgttt
    14701 aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt ttcaaaatag
    14761 gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg cagacgggtg
    14821 gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat gaccaacagt
    14881 cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt gacatgggtg
    14941 gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac caggctctca
    15001 atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa tttgttgctt
    15061 ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca gctgatttca
    15121 agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa acaaaccaat
    15181 gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat cttggtggca
    15241 atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt acggtacaag
    15301 gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg actgttctcc
    15361 cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc gaacaattta
    15421 tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc ggcaataaag
    15481 gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt gggggtttta
    15541 ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg caacgaaatg
    15601 ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata tttattgaga
    15661 ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag tttcatctct
    15721 tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc actttgcaag
    15781 atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg cacgcctgtc
    15841 tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc attttcgaca
    15901 aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc gtgtcctaat
    15961 acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc aacagcatta
    16021 tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat taaaagcgca
    16081 cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat tttttataaa
    16141 aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa attgtcgaat
    16201 agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat atttgcttga
    16261 acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca gaaatgtgtt
    16321 tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg ctcgtcaccg
    16381 ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg gggcaaaaat
    16441 gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt tccgaatggt
    16501 ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt aaaccagcgc
    16561 atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc gaaagcgggc
    16621 gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga aaacgccata
    16681 cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc ggtacgctcc
    16741 tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga aaccttcctc
    16801 aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc ttctctgttt
    16861 aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg cgtggcgctc
    16921 tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt gatgttctcc
    16981 attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg gggccttggg
    17041 gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa accgacactg
    17101 gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat ccgcatggtt
    17161 tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc gcaggaacag
    17221 cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca ggccgtacaa
    17281 attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg ccacaccggc
    17341 aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca ggtcatggag
    17401 cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat cggtcatcgc
    17461 atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg catcgaacct
    17521 ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat gaagaaagca
    17581 tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat cgctcgtgaa
    17641 ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa agacggtgag
    17701 aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga gttgctggaa
    17761 tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa agtgatgatg
    17821 aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga aggcgtgggc
    17881 cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg cgcactgctt
    17941 gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat gaaaggtttt
    18001 gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac gctgggtgcc
    18061 gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa cgaatatgcc
    18121 aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct cggcttccgt
    18181 ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga gtgtgaagca
    18241 gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat cccgttcgtg
    18301 cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg gctgaaacgt
    18361 ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc cttgctggcc
    18421 gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat gacgcagctg
    18481 gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga gcgcaacgat
    18541 gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg caaatatgtc
    18601 gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt gatggaagag
    18661 gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt aagcctggct
    18721 gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta tgtgatcccc
    18781 gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg tataatatct
    18841 tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt ttcgattacc
    18901 acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa agccgccatc
    18961 tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca tgatcgttca
    19021 cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg atgctgcaat
    19081 aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat aatatctttt
    19141 ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc gccgctgtaa
    19201 taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa ataccccata
    19261 aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa ggtcttgcgt
    19321 gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt atgcataaag
    19381 cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt ggaaccataa
    19441 acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt ggggattgtg
    19501 gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt ctctagtaaa
    19561 ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg ctgctgctca
    19621 agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg agcgccaatt
    19681 aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg cgcaggcatc
    19741 ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc actggcgaga
    19801 atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga ggtaaacagc
    19861 accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat attgtcggcg
    19921 ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac gccgacaatt
    19981 tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg acgcgtttgt
    20041 ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac ccacaccagt
    20101 tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt caggcaggca
    20161 agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg atcgcctgat
    20221 tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc gagcgcgcta
    20281 taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat cgcacgagcg
    20341 gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg acgatacgcc
    20401 gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg aaacagaaca
    20461 tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa tcaataccga
    20521 aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc acccgacagg
    20581 tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca tcctgcttat
    20641 aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg ggattttccg
    20701 tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct aatttgatgt
    20761 cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc catctttcag
    20821 caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta ccggctggtt
    20881 tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc gtgggtattg
    20941 gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct gcatatcacg
    21001 catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg cggagaaatc
    21061 cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg caatggctaa
    21121 atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt caccaacgat
    21181 catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg gcaccatatg
    21241 cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg ggtgttgttt
    21301 aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac ccaatccaca
    21361 aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc ccatcaggcc
    21421 gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa tattaaagcg
    21481 atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag cgtaatgatg
    21541 cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa cgccatcagc
    21601 cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag tgaccttgtt
    21661 tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc ccgcctgctc
    21721 ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag atgcgtgttg
    21781 aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt cggttaagaa
    21841 ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa agcctggaat
    21901 gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac atccggcact
    21961 gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca gtgcggcaac
    22021 gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg acatgcgtta
    22081 tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat tagcggctca
    22141 actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg tagtcggcat
    22201 agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt acgccgttgc
    22261 cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga tcggatttca
    22321 gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg ccttcggcaa
    22381 ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg accattttca
    22441 ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct ttgctcaggt
    22501 ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga cgcggagcaa
    22561 cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac ggttcctgaa
    22621 caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga gcgaacgcta
    22681 acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc accgccgtgg
    22741 catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga gctttaccac
    22801 gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg gcagcaatgc
    22861 tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca taaatacatt
    22921 ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg gcatacacct
    22981 gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga ttatcgctaa
    23041 agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca gttcagggct
    23101 ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc gagaatgatg
    23161 tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag tggcgcgctc
    23221 tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc cgcgccgaga
    23281 atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca gagcacggct
    23341 ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat caccagtgta
    23401 ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag aatttctccg
    23461 accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa gatcagtaaa
    23521 tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag aggcagtgca
    23581 tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga tagcacgtct
    23641 ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc tatctcatcg
    23701 tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg gctgattttg
    23761 gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc ctgttcttca
    23821 ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg catacggaac
    23881 catctccgat ggaaggtaaa aatggcctga aaatagtgtg
//
LOCUS       DAJTUI010000060        23824 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTUI010000060 DAJTUI010000000
VERSION     DAJTUI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.060"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(46..918)
                     /gene="rluF"
                     /locus_tag="OO464_002544"
     CDS             complement(46..918)
                     /gene="rluF"
                     /locus_tag="OO464_002544"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6227651.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1266..2213
                     /locus_tag="OO464_002545"
     CDS             1266..2213
                     /locus_tag="OO464_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR6227652.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2283..3089
                     /locus_tag="OO464_002546"
     CDS             2283..3089
                     /locus_tag="OO464_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6227653.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3099..3506
                     /locus_tag="OO464_002547"
     CDS             3099..3506
                     /locus_tag="OO464_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR6227654.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3506..4000
                     /locus_tag="OO464_002548"
     CDS             3506..4000
                     /locus_tag="OO464_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR6227655.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4066..4863
                     /locus_tag="OO464_002549"
     CDS             4066..4863
                     /locus_tag="OO464_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6227656.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            4874..5662
                     /locus_tag="OO464_002550"
     CDS             4874..5662
                     /locus_tag="OO464_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6227657.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVSLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5714..6943
                     /gene="sorE"
                     /locus_tag="OO464_002551"
     CDS             5714..6943
                     /gene="sorE"
                     /locus_tag="OO464_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR6227658.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7325..8014
                     /gene="pepE"
                     /locus_tag="OO464_002552"
     CDS             7325..8014
                     /gene="pepE"
                     /locus_tag="OO464_002552"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6227659.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8105..9736)
                     /locus_tag="OO464_002553"
     CDS             complement(8105..9736)
                     /locus_tag="OO464_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6227660.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(9956..13639)
                     /gene="metH"
                     /locus_tag="OO464_002554"
     CDS             complement(9956..13639)
                     /gene="metH"
                     /locus_tag="OO464_002554"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR6227661.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            13839..14663
                     /gene="iclR"
                     /locus_tag="OO464_002555"
     CDS             13839..14663
                     /gene="iclR"
                     /locus_tag="OO464_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR6227662.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            14980..17164
                     /gene="espL4"
                     /locus_tag="OO464_002556"
                     /pseudo
     CDS             14980..17164
                     /gene="espL4"
                     /locus_tag="OO464_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17133..18870)
                     /gene="aceK"
                     /locus_tag="OO464_002557"
                     /pseudo
     CDS             complement(17133..18870)
                     /gene="aceK"
                     /locus_tag="OO464_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19054..20358)
                     /gene="aceA"
                     /locus_tag="OO464_002558"
     CDS             complement(19054..20358)
                     /gene="aceA"
                     /locus_tag="OO464_002558"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR6227663.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20388..21989)
                     /gene="aceB"
                     /locus_tag="OO464_002559"
                     /pseudo
     CDS             complement(20388..21989)
                     /gene="aceB"
                     /locus_tag="OO464_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22258..23187)
                     /gene="metA"
                     /locus_tag="OO464_002560"
     CDS             complement(22258..23187)
                     /gene="metA"
                     /locus_tag="OO464_002560"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6227664.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23344..23787
                     /locus_tag="OO464_002561"
     CDS             23344..23787
                     /locus_tag="OO464_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6227665.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5862 a         6075 c         6045 g         5842 t
ORIGIN      
        1 ctgtccgagc tctttttgga cgttttcatc agtctgaggc gttaatcagc gtcccttctt
       61 tttacgcccc ggcgaggtaa aacgcttacc gttggacgcc gggcgaccgc ctttttccgc
      121 cgttttttcc atcttaacga ctggacgttt gatgcccgct gttttcggtt tggccttcgc
      181 tttaggtttt gcctcggaag aggaattttc gatgagctta aagaggtcga ttaactcatc
      241 gtcggttaaa tcgcgccact cccccagcgg aatgccgctt aagctgacgt tcataatgcg
      301 cgtacgttcc agctttttca cttcatagcc gaaatgctcg cacatgcgcc ggatctgacg
      361 gttcagcccc tgcactaggg taatgcggaa gacaaacggc gcttcttttt tgactttgca
      421 ctttttcgtc accgtcccga ggatcggcac ccccgccccc ataccacgaa taaattcatc
      481 ggtaatcggt ttatcgaccg tcaccagata ctctttctca tgatcgttac ccgcacggag
      541 gatcttattc accagatcgc cgtgattggt gaggaaaatc agcccctggg agtctttatc
      601 caggcggcca atcgggaaca cgcgtttgct gtggttaacg aaatcgacaa tgttatcgcg
      661 ctcgccatct tcggtggtgc tgacaatacc gacgggcttg ttcagggcga taagtaccaa
      721 atcttcggct tcccgaggct caatcaactg accgtttact ttcacaatgt cgccgggttt
      781 cacctgatcg ccaatggtgg ctcgcttgcc attaaggaac acattgcctt gctcgatata
      841 gcgatccgct tcgcggcgtg agcaaattcc gctttcgctg atgtatttat ttaaacggac
      901 tgatgagtcg ggcagcataa attctcctgt aaaacgcgga atataccgta cctcttagcg
      961 gggaaaaaca gggttatgcg gcaacgtcga aattaaatgt gatctgccac gccttttatc
     1021 acaaaagtgt ctccggaagt acgatgcaaa tttgcctggc aaaataagga taatcaacat
     1081 attacgaatc gctgcacacc tgtgctgatg actactacgc agagaaacaa atgtgcaaga
     1141 gaaagcgcta tttctcccca tgcaaagagt gaaaaatcgc tttgaaaggc cctcatgatg
     1201 gggttatatc agatcaagcg gtgagaaagc gtatttgcac aaatgtgcag aaccaggagg
     1261 cggcaatgga aaacagtgac gatatccgtt tgattgtgaa gattgcccaa ctctattacg
     1321 aacaggatat gacgcaggcg caaatcgcgc gcgaactggg tatttaccgc accaccatca
     1381 gccgcttgct taaacgaggc cgcgatcagg gaatggtcac catcgccatc aactatgact
     1441 acaacgaaaa tctctggctg gagcagcaac tgaagcaaaa gtttggcctg aaagacgttg
     1501 tggtggtgtc gggaaatgat gaggatgaag agactcaact ggcgatgatg gggttacacg
     1561 gcgcgcaact gctggatcgc ttgctggagc ctggcgatat tgtcggtttt tcctggggtc
     1621 gcgcggtgag cgcactggtt gaaaacttgc cgcaggcggg gcaatcgcgg cagttaatct
     1681 gcgtaccaat tattggcgga ccgtccggta aactcgaaag ccgctatcat gtaaacacat
     1741 taacctacag cgcggcagcg aagctgaaag gggaatcgca tctcgcggat tttccggctc
     1801 tgctggataa cccattaatt cgtaatggga tcatgcagtc tcagcacttt aaaaccatct
     1861 ctgcctactg ggataatctg gatatcgccc tggtgggaat tggctcaccg gccattcgcg
     1921 acggcgctaa ctggcatgcg ttttatggtg gtgaagagag tgacgacctg aatgcccgcc
     1981 aggttgctgg cgatatttgc tcgcgctttt ttgatattca cggcgcaatg gttgaaacga
     2041 atatgagcga aaaaacgctc tctatcgaaa tgaataaatt aaagcaggcg cgatattcca
     2101 ttggcattgc catgagtgaa gaaaaataca gcggaattat tggtgcactg cgtggaaaat
     2161 atattaattg tctggtaacg aatagcagca cagctgaact attactgaaa taaaaatctt
     2221 cccgatgttt accgtaataa aaattacggc gggtccctat tatctaaaat caggagtctg
     2281 ttatgcaaac gtggttaaat ttgcaggata aaatcattat tgtcaccggc ggcgcatccg
     2341 gtattggtct ggcgattgta gaggaattat tagcacaagg cgcgaatgta cagatggtcg
     2401 atattcacgg tggcgatggt caatatgaag gtcataaagg ctatcagttt tggccgaccg
     2461 atatttctag cacaaaagag gtaaatcata cggtagcgga aattatccag cgttttggtc
     2521 gcatcgacgg tctggtcaat aacgccgggg tcaatttccc gcgtctgctg gtcgatgaga
     2581 aagcgcctgc cgggcagtat gaactcaacg aagctgcatt cgaaaaaatg gtcaatatca
     2641 accagaaagg tgtttttctg atgtcgcagg cggtggcgcg acagatggtc aaacaacatg
     2701 atggcgtgat tgtgaatgtt tcctcagaaa gtgggctgga aggctcagaa ggacaaagct
     2761 gttacgccgc taccaaagcc gcgctcaata gcttcacgcg ctcctggtcg aaagagctgg
     2821 gtaaacacgg tatccgtgtg gtcggtatcg cgcccgggat tctggaaaaa accgggctac
     2881 gcacgccgga atatgaagag gcgctggcgt ggacgcgcaa tatcaccgtc gagcagctgc
     2941 gtgaaggcta taccaaaaac gccattccta ttgggcgcgc cggaagatta gcagaagtag
     3001 ctgattttgt ttgttatctg ttgtctgaac gcgccagcta catcaccgga gtaaccacta
     3061 acattgcggg cggcaaaacg cgcggctaag gaggctttat ggtcaacgcc attttttgtg
     3121 cccacggcaa attagcttgc gccatgctgg aatcggttca gatggtttac ggcaacgcca
     3181 atgttgaagc cgttgcattt gtgcccggtg aaaacgccgg agacattgcg gtaaagctgg
     3241 aaaagttagt aagcattcac aatcaagatg agtggctgat tgccgtggat ttacagtgcg
     3301 gcagcccgtg gaatgcggca gcaatgcttg cgatgcgcaa tcctcgtttg cgggtaatca
     3361 gcggcctttc tctgccgctg gcgctcgaac tggttgataa ccaggacagc atgaatgttg
     3421 atgaactgtg tgaacacctg acgcaaatcg caaagcaaac ctgcgttgtc tggaaacaac
     3481 tggcaacgac agaggaggat ttctgatgaa cattacgctc gcccgcattg atgaccgtct
     3541 gatccacggc caggtcacta ccgtctggtc gaaagtggct aacgcccagc gcattattat
     3601 ctgcaatgac gaagtttata acgatgaagt gcgccggaca ttattgcgtc aggctgcgcc
     3661 accgggaatg aaggttaacg tggtcaatat tgaaaaagcc gtcgcggttt atcacaaccc
     3721 gcaatatcag gacgaaaccg ttttttatct tttcactcgc ccacaggatg ccttagcgat
     3781 ggttcgccag ggggtaaaaa taggcaccct gaatattggc ggcatggcct ggcggcccgg
     3841 taaaaaacag ttaaccaaag cggtttcatt agatgatgat gatattaacg catttcatga
     3901 gttaaataat cttggtgtca tactggattt acgcgtcgta gcgtcagatc cctcaatcaa
     3961 tattatcgac aaaataaacg aacagttaat cgcaaattaa ataatagcgc ctgtgtacat
     4021 atgctttaac aggcagggat gacgacactt aaaaggtgac aaatcatgga aataagtacc
     4081 ctacaaatca ttgctatatt tcttttttcc tgtattgccg gaatgggcag cgtgctggat
     4141 gaatttcaga ctcatcgccc gttaattgcc tgtacggtga ttggtttaat tctcggtgat
     4201 ttaaaaaccg gaataatact cggcggtacg ctggaattga tagctctctg ctggatgaac
     4261 gtcggcgcgg cgcaatctcc ggattctgca ctcgccagca taatctccgc cattctggtt
     4321 atcgttggtc agcaaagcat cgccaccgga atcgccatcg cgttgcctgt ggctgcggca
     4381 ggccaggtgc tgacggtgtt tgcccgtacc atcaccgtag tgttccagca cgcggcggat
     4441 aaagcagcag aagaggcgcg gtttcgcact ctcgatattc tgcatgtctc cgcgcttggc
     4501 gtgcaggcgc tgcgcgtcgc cattccggca ctgattgtct cactgttcgt cagcgccgat
     4561 atggtcagca atatgttaaa cgccattccc gaatttgtga cccgaggact acagattgct
     4621 ggcggtttta tcgtggtggt cggttacgcc atggtgctac gtatgatggg cgtgaaatat
     4681 ttgatgcctt tctttttcct cggtttcctc gcaggtggct acctcgatct cagcctgctg
     4741 gcgttcggtg gcgtcggcgt gatcatggcc ctggtctaca tccagctaaa tccacagtgg
     4801 tgtaaagctg aacctcaact ccaggccacc gcctccaccg cccttgacca acttgacgat
     4861 taacggagcc atcatggaac agagaaaaat tacacgcagc gatctggtga gcatgtttct
     4921 gcgctccaac ctgcaacagg cgtcctttaa ctttgaacgt attcacgggc tgggcttttg
     4981 ctacgacatg atccccgcca tcaagcgact ttacccatta aaagaggatc aggttgcggc
     5041 gctcaggcta cacctggtgt tcttcaatac cacgccagcc gtatgtggcc cggtcatcgg
     5101 cgcggaaatt gatgacggta ccatcaacgg catcaaagtc ggtctgatgg gaccattggc
     5161 aggagttggc gatccactgg tctggggaac gctgcgcccg attaccgccg cgctcggcgc
     5221 atctctggca ctttcgggca acattctcgg cccgctgctg ttcttcttta ttttcaacgc
     5281 ggtgagtctg gcgatgaagt ggtatggcct acagctcggc tttcgcaaag gggtgaatat
     5341 cgtcagcgat atgggcggga atgtgctgca aaaactcacc gaaggcgcgt cgattctcgg
     5401 actgtttgtg atgggcgtgc tggtgaccaa atggacgtca atcaacgtac cgttggtggt
     5461 ttcacaaacg catgccgccg atggctccac cgtcaccatg accgtgcaga acattctcga
     5521 ccaactttgc cctggtttgc tggcgctcgg tctgacgcta ctaatggttc gtctgctcaa
     5581 caaaaaaatt aacccggtat ggctgatttt cgccctgttt ggcttaggga ttatcggcaa
     5641 tgctctgggc ttcctgtcct gattcttcgc cccggcacga ctgccgaggc catcgctcaa
     5701 catgaggtgg tttatgaaaa caacagctct gcgtctttat ggtaaacgtg atttacgcct
     5761 ggaaaccttt gaccttcctg aaatgcagga ggatgaaatc ctcgcaacgg tggtcactga
     5821 cagcctgtgc ctctcttcct ggaaagaggc caatctgggt gaaaaccata aaaaagtacc
     5881 cgacgatgtg acgaccaacc caatcatcat cggccacgaa ttttgcggcg atattctggc
     5941 cgtgggtaaa aagtggcagc acaaattcca gccgggtcag cgttatgtga ttcaggccaa
     6001 cctgcaactc cccgaccgcc cggattgccc cggctactcc ttcccgtggg tagacggcga
     6061 agcgacgcat gtggttattc ccaacgaggt catggaacaa tattgcctgc tggcatacga
     6121 cggcgaaacc tattttgaag gctcactggt tgaaccgctt tcctgcgtga ttggcgcgtt
     6181 caacgccaac tatcatcttc aggaaggtag ttataaccac acgatgggga ttcgcccgca
     6241 agggcacacg ctgatcctcg gcggcaccgg accaatggga ctgttggcga ttgattatgc
     6301 gctgcatgga ccagttaacc cgtcgctgct cgtcattacc gataccgaca acgacaaatt
     6361 gagttatgcg cgcaagcact atccatcaga accgcaaaca ctgattcatt atctcaatgc
     6421 caccgatgca gcatttgata cgttaatggc gctgagtggc ggtcacggct tcgatgatat
     6481 tttcgtcttt gtgcctaatg aaggactggt gactctcgcc tcttccttgc tggcgacaga
     6541 tggttgcctg aatttcttcg ccggaccgca ggataaacat ttcagcgcgc caattaattt
     6601 ctacgatgtg cattatgcat ttacccacta cgtgggcacg tcaggcggca ataccgacga
     6661 catgcgcgcg gcggtcaaac tgatcgaaga gaaaaaagtg caggccgcaa aagtggtaac
     6721 acatattctt gggctgaatg ccgcgggcga aaccacgctt gaattgcctg ccgtcggcgg
     6781 cggcaaaaag ctggtgtata ccgggaaata cctgccgctg acgtcactca cgcagattca
     6841 ggatcaagca ctggcggcga ttctggcgcg tcatcagggg atctggtccg gtgaggtgga
     6901 gcaatacctg ctcgcccatg cagaggcaat ttcccatgat taatcgcgct acttaactct
     6961 gcatgtcgct ggctggtcgc cccagcaact tcgcgaccgg atgtgcggct gattgctgaa
     7021 gcagcacaat ttgcacgaga aggataaaaa atatcagcgc cttaaggggc gctcaaattg
     7081 gtggcaaagt acacattgtt tatgccggat gcggaatgaa gccttattcg gtctacataa
     7141 ttttgaaaat ccaatatatt gcagttatct ggtaggccag gtaagcgtag cgcatcaggc
     7201 aatgctgtgt ttgtcatcag tctcagcgcc cggtgcaata cgtgacatcg tgagccatgt
     7261 cagtaagtgg ttaaattcgc agtggtagca tgccttccgt gatttcttac taaccggaga
     7321 agtaatggaa ctgcttttat tgagtaactc gacgctgccg ggtaaagcct ggctggaaca
     7381 tgcactgccg ctaattgctg aacagttgca gggtcgccgc tcagcggtgt ttatcccttt
     7441 cgctggcgta acgcagacct gggatgatta cacagagaaa acggctgcgg ttctcgctcc
     7501 gctgggtgtt tctgtcaccg gtattcatag cgttgtcgat cccgttgccg cgattgaaaa
     7561 tgcggaaatt gtgattgtcg gcggcggtaa tactttccag ttgctgaaac agtgccgcga
     7621 gcgcgggctg ctggctccaa ttactgacgt ggttaaacgt ggcgcgctgt atattggctg
     7681 gagcgcaggc gctaaccttg cttgcccaac cattcgtacc accaacgata tgccgattgt
     7741 cgatccgcaa ggtttcgatg cgctaaatct gttcccgttg caaatcaacc cgcacttcac
     7801 caacgcgctg ccggaaggcc ataaaggtga aacccgtgag cagcgtattc gcgaactgct
     7861 ggtcgtcgcg ccagaactga cgattattgg tctgccggaa ggtaactgga tcacagtgag
     7921 taaaggtcac gctacgctgg gtggcccgaa caccacttat gtgtttaagg ctggtgaaga
     7981 agcggttccg ctggaagctg gtcaccgttt ttaagtgaat tgccggatgc atgatccggc
     8041 atcttgttca tagtgcctga tgcgacgctt gacgcgtctt atcaggccta caggattcac
     8101 aatattaata ctcgtcccgt ccttcatctt catccggctg ttccagcaca ctgtaagcca
     8161 ccgaacaaaa cagcgagttc aggcgctgca tatctcccag taagcctaaa tgtagcgaac
     8221 tggtttcaat gctctgcacg ttttgctgat gcaggcgatc gacgtgggcg tgggaatagc
     8281 ggcgattaag aatgcgaaaa cgatgtttgc tgcgacgcaa acgacgagcg ctggtgacat
     8341 cgccagagaa gaacaccgac attgccagct ttaaattact gagcaattgc tcatagagcg
     8401 catccagttc cttcaaccca tcaagcgaaa atgcccgccg tgctgccagc gatttatcgg
     8461 caatttcgct gcccatgcgc tcgacgatat cggaggcctg ttcaaggttg agcgacattt
     8521 cgatgatctc cgcccagcgg cgcgactctt cctccgccaa ctcttctttt ggcatccgcg
     8581 ccagatacag cttaatggcg gtgtagagaa cgttgatatc atctgccagc ttacgcagct
     8641 ctttctcctg ccgtggctcg ccgtgcatca ctttattcag cccttccatc atctgttcca
     8701 tggcgtcgcc aatgcgcagg gtttcgcgcg cggcgttcgc cagagcaagc gtgggcgtgt
     8761 ccagcgcgct gacatccaga tgtttaggcc gtagctgggt atccagttcc ggttcatcgc
     8821 gaataatcgt tttgcaaaac cgtgccatcg ggtcaacaaa tggcagcatg accaggcaac
     8881 gtacaaggtt gtagaagacg tggaaataga tcaccagttc cgcttttggc aatgacagct
     8941 tccccattgt ctctgccagc aaatggacaa acggcaggat aatcaggcta cccaccagct
     9001 taaacagcag actacccagc gcgacacggc gggctgcggc attggcggca ctgttgttga
     9061 gcatcgccag caggccggaa ccgaggttag ctccaatcac cagacagagc gccacgggga
     9121 aggagataat gcctgcagcg gtcagagtcg cggtcagcag tacagcagca aggctggagt
     9181 agctgataat ggcgaacatc gcgccaatca gcgcatccag cagaatatcg ccggtcagcg
     9241 aggcaaagat cacctgaacg ccgtttgcct gggtgatcgg cgttacggcc tgcacaatca
     9301 actccagcgc cagcaaaatc agcccaagac caataccgac gcggcccagt tgcccggcgc
     9361 gtgactgttt gcgtccgagg aaaaaaatca cgccgataaa aataagtaac ggtgacagcc
     9421 aggataagtc gaaggtgaga atacgcgcca ttagcgccgt cccgacatct gcccccagca
     9481 caatgaccag agccggtgcg agggctacca gatcctgagc gacgaacgag gtcaccagca
     9541 tggtggtggc attactgctc tgtaccagtg cggtaacgcc gatccccgcg caaaaggcga
     9601 gcggcttttt ttcgacgctc cggctaagga cagtacgcaa acgcgcgccg aagacgcgca
     9661 ttacgccggt tcgaacaata tgagtccccc agaccagcag ggcgacggca gaaagcaggt
     9721 gaagcagcgt taacacgagt tcagattctc cttatcgtta tatgttcctg aagcaattga
     9781 atgaccaaag gaccggaaac acgctgactg gcatctttac tgtgcctttt gacattcgtt
     9841 tttccggcta agattattcc agcctgtttc cagtataagt gtttcatatt aataaagaga
     9901 cagggcactc attgagcgcc ctgtttgttg taaggaaaag tgacagattt gtgaatcagt
     9961 ccgcgtcata ccccagattc ggtgccagcc agcgctcaac atcggagacg ctcatacctt
    10021 tacggcgggc atggtcttca acctgatcgc gctgaatttg cgccaccgcg tagtacttgc
    10081 tgtccggatg actgaaatac caacccgaaa ccgatgcacc aggccacatc gcgaaagatt
    10141 ctgtgagttt catgccagta tgtttttcca cttccagcag ctcccagatg gtggcttttt
    10201 ccgtatgttc cgggcaggcc ggatagcccg gtgccggacg gatgccctgg tagttttcgc
    10261 ggatcagttc ttcgttgctg aggttctcgt tcggcgcata gccccagtag actttacgca
    10321 cacgctcatg gagatactcc gcaaaggctt cggctaaacg gtcggcaagc gctttcacca
    10381 tgattttgtt gtaatcatcg tgctgcgctt caaaggcatc agccagtgcg tcctcttcca
    10441 gcccgccagt cacggcaaat gcgccgatgt aatctgcttt accagaaagc ttcggcgcaa
    10501 cgaagtcagc gagacagtag ttagcgaatc ccgttttttc ggtctgttga cgcagatggt
    10561 ggctgacgtt gatcacatgg gtgcgcgttt cgtcacggta gatttcaatg tcatcgccca
    10621 cacggtttgc cgggaacagg cccaccacgc cacgcggatt cagcgttttc tcggcgctta
    10681 atttatccag catgtcgttg gcgtctttaa acagccgctg cgcctcaacg cccaccactt
    10741 catcttccag aatgcgagga tacttcccgg ccagcgacca ggtcataaag aacggtgtcc
    10801 agtcgatgta attacgcagc gtttcgatgc tggcttccac ttcctgcacg ccgagacggt
    10861 gcgccaccgg cggcgtgtaa gcctgccagt caaatgcgaa atcgttatcg cgcgccgctt
    10921 ccagcgtgac cggtggtgtg cgtggtttct tgcgcccgtg ctgaatacgc acggtttcgt
    10981 actccttgca ggtacgagcg acaaaatcat cacgctgagt atcggaaagc agcgccgcta
    11041 ccacaccaac ggtgcgcgag gcattctgca catacaccgt agggccgctg tagttctgct
    11101 cgattttcac cgccgtgtgc gcttttgagg tggtcgcgcc gccaatcagt aacggaatgg
    11161 cgaagccctg acgctccatc tctttcgcca cgttaaccat ctcgtccagc gacggcgtga
    11221 taagccccga aaggccaatc aaatcagcat tcacttcttt ggcgatacgg agaatttttt
    11281 ccgcaggcac cataacgccg agatcgacaa tttcgtagtt gttacattgc agcaccacac
    11341 caacgatatt tttaccgatg tcgtggacat cgcctttcac ggtggcgatc accatcttgc
    11401 cgttggtttt accctgctct ttgctggctt caataaacgg ttcgagatac gccactgcct
    11461 gtttcatgac gcgcgccgat ttgaccacct gtggcaggaa cattttgcct tcgccaaaca
    11521 gatcgccgac cacgttcatg ccgtccatca acgggccttc aatcacttca atcgggcgcg
    11581 tagcctgctg gcgggcttct tcagtatcct gctcgataaa ctcggttatg cctttgacca
    11641 gcgagtattc cagacgttta ttcacgtccc acgagcgcca ctccgcttgc tgggcgttag
    11701 cagtgtcgtc cgctttactg ccgcgatatt tctcggcaag ctccagtaaa cgctcagtgc
    11761 catcgtcacg acgattaaga atcacatctt ccaccgcgtc gcgcagttca gcgggtaggt
    11821 cgtcgtaaat cgccagttgc ccggcgttga cgatccccat atccatgcca ttgcgaatag
    11881 cgtagtagag gaacactgcg tgaatggctt cgcgcaccgg atcgttgcca cggaatgaga
    11941 aagaaacgtt agatacgccg ccggaaatca gcgcgtgcgg cagttcgcgt ttgatgtctt
    12001 cacacgcgcc gataaagtcc tgcgcgtagt tgttgtgctc ttcaataccg gtggcgaccg
    12061 cgaagatgtt cgggtcgaag atgatatctt ctggcgggaa gccaacctct tcggtgagga
    12121 ttttgtacgc ccgacggcaa atctcgattt tccgtgcgcg agtatcggcc tgtccctgtt
    12181 cgtcaaaggc catcaccacc actgccgcac cgtagcgacg caacaatttc gcgtgatgga
    12241 taaaggcgtc gacgccctct ttcatcgaga tagagttaac aatgcctttg ccctggatac
    12301 acttcagacc tttttcaatg acatcccatt ttgaggagtc gatcataatc ggcacgcgag
    12361 cgatatccgg ttcaccggca atcagattga gaaaacgcac catcgccgct tcggcatcga
    12421 gcatcccttc atccatgttg atatcgataa tctgcgcgcc gttttccacc tgctgacgcg
    12481 ccacatccag cgcctcgctg tatttctctt ctttgatcag gcgcttgaac ttagcagagc
    12541 cggtgacgtt ggtgcgttca cccacgttca caaacaggct atcgtcgcca atgttcagcg
    12601 gctccaggcc agacaaacgg caggctacgg gaatttccgg cagtttgcgc ggcgctaatc
    12661 cttctactgc acgactcatc gctgcaatat gttgcggcgt ggtcccgcag caaccaccga
    12721 cgatattgag aaagcccgct tgcgcccatt cacgtatctg ttttgccatc gtgtcggcgt
    12781 cgagatcgta ttcaccaaag gcgttaggca gcccggcgtt cgggtgggcg gtgacgtagc
    12841 attccgcaat ccgtgatagt tcctgcacgt actggcgcag ttcatcgggc cccagcgcac
    12901 agttcaggcc aaaggtcagc gcttcggcgt ggcgcaatga gttgtaaaat gcttcggtgg
    12961 tctgcccgga gagcgtgcgc ccggaggcgt cggtgatggt gccggagatc ataatcggca
    13021 gctcaacgcc cagcgcttca aactccgttt tcaccgcaaa gaccgccgct ttggcgttaa
    13081 gggtgtcgaa aacggtttca atcaggatca gatccgcacc accttccacc agcgctttgg
    13141 tggattcgcg ataggccgcc gccagctggt caaaagtgat gttgcggaat gccggatcgt
    13201 tgacgtccgg agaaatagac gccgtgcggt tggttgggcc aagaacaccg gcaacgtagc
    13261 gcggtttctc cggcgtgcgc gcggtccact cgtcagcaca ggcgcgcgcc agtttcgccg
    13321 ccgcaaagtt gatttccgcc gacagggatt ccatctggta atccgccatc gcaatggtcg
    13381 tggagttgaa ggtgttggtt tcgatgatat ccgcgcccgc ttcaaagtag gcgttgtgga
    13441 tagcggcgat cacttccggt ttactgagta ccagcaggtc gttgttgcct ttgaggtcgc
    13501 atggccagtc ggcaaagcgt tcaccacgaa aatcggcttc gttcagtcga tagctctgga
    13561 tcatggtgcc cataccgccg tccaacacca gaatacgttc atttaactgc gcacgcagtt
    13621 gttccacttt gctgctcaca cttgctcccg acacgcttaa cccagattta ataaaaattc
    13681 aacaaaccat actggaataa acgcatctgt ggtaaaagcg accaccacgc aacatgagat
    13741 ttgttcaaca ttaactcatc ggatcagttc agcaactatt gcattagcta acaataaaaa
    13801 tgaaaataat ttccacgata cagaaaaagg aggctgtcat ggtcgcaccc attcccgcga
    13861 aacgcggcag aaaacccgcc gttgccaccg caccagcgac tggacaggtt cagtctttaa
    13921 cgcgtggcct gaaattactg gagtggattg ccgaatccaa tggcagcgtg gcactcacag
    13981 agctggcgca acaagccggg ttacccaatt ccacgaccca ccgcctgcta accacgatgc
    14041 aacagcaggg tttcgtgcgt caggtcggcg aactgggaca ctgggcaatc ggcgcacatg
    14101 cctttatggt cggcagcagc tttctccaga gccgtaattt gttagcaatt gttcacccta
    14161 tcctgcgcaa tctaatggaa gagtctggcg aaacggtcaa tatggcggtg cttgatcaaa
    14221 gcgatcacga agcgattatt atcgaccagg tacagtgtac ccacctgatg cgaatgtccg
    14281 cgcctatcgg cggtaaattg ccgatgcacg cttccggtgc gggtaaagcc tttttagccc
    14341 aactgagcga agaacaggtg acgaagctgc tgcaccgcaa agggttacat gcctataccc
    14401 acgcaactct ggtgtctcct gtgcatttaa aagaagatct cgcccaaacg cgcaaacggg
    14461 gttattcatt tgacgatgag gaacatgcgc tggggctacg ttgccttgca gcgtgtattt
    14521 tcgatgagca ccgcgaaccg tttgccgcaa tttctatttc cggaccgatt tcacgtatta
    14581 ccgatgatcg cgtgaccgag tttggcgcga tggtgattaa agcggcgaag gaagtgacgc
    14641 tggcgtacgg tggaatgcgc tgactttttc tggcgggcag aggcaatatt ctgcccatca
    14701 tacctgagtg gcaatagaat aagggtgtct gttaatcgca ttgacgccaa aataacttaa
    14761 tgtcatacac ttcacacact tttattaact cagcattatt tttaaacatc aaaccactta
    14821 attattaaca aaatcgcaag atctcattcc accgaaactt ttacggctgc attatgagat
    14881 tgagcgattt atgatatttt tttagttgta atcatattaa attttttcac tgttctgata
    14941 gttaaaattc aagacatcaa taaacaatga gatatttaaa tgattactcg tattcctcgt
    15001 agttctttct ctgcaaatat taataataca gcccagacaa atgaacacca aaccctgagt
    15061 gaattgtttt ataaagaact cgaggataaa ttttctggca aggagctggc gacgcctcta
    15121 ttaaaaagct tctcagagaa ctgtagacaa aatgttcgtc atatctttag caacaaggat
    15181 tttgtcatta aattttccac gtccgtctta caagctgata agaaagaaat tacgatgatt
    15241 aataaaaacg aaaacacgac actcactcaa accattgcta caatatttga agaataccta
    15301 atggaaattt tacctcaacg ctcagacact cttgataaac aagaattaaa ccaaaaatca
    15361 gatagaaaag aaaaagaatt cccaagaatt aaacttaatg gtcaatgtta tttcccgggg
    15421 cgccccaaaa ccgtatagta taccgacaca ttgctgcaca atatattaat gatatttatc
    15481 agaatgttga ttacaaaccc catcaagatg attactcttc agctgaaaaa tttctcactc
    15541 acttcaacaa aaaatgcaaa aaccagactt tggcgttgat ttccagccgt cctgaggggc
    15601 gttgcgttgc tgcctgcggt gatttcgggc tagttatgaa agcatatttt gacaagatgg
    15661 aatcaaatgg catcagtgtt atggcagcca tattactggt ggataaccat gctttgacgg
    15721 tccggctaag aataaagaac acaactgaag gatgtaccca ttacgtggtt tcggtttatg
    15781 atcctaatgt aactaacgat aaaataagaa ttatgagcga aagcaaagag gatattaaac
    15841 actattctct gatggatttt atgaatgtag attatagcct cctgaaatgg tcaaatgatc
    15901 atgttattaa ccaatctgtt gcaataattc cagcacttcc gaaagaacag ctattgatgt
    15961 taaaaggaac tgtggatgaa ataacccctc cattatcacc agcaacgatg aatttgctaa
    16021 tggcaattgg tcagaatcac caacttacgc aactgatgat tcagctccag aaaatgccag
    16081 aactacatag aacagaaatg ttgactgcct ataatagtat taacctcccc ggtttatatt
    16141 tggctataaa ttatggcaat gcggatatcg ttgagactat tttcaattca ttgtcagaaa
    16201 caggatatga agggttactc tcgaaaaaaa atctcatgca tattctggag gcaaaagata
    16261 aaaatggttt ttctggatta tttttagcga tatcacgtaa ggataaaaat gttgtaacct
    16321 cgattctgaa cgtcttacct aaactggccg caacacatca tttagataat gaacaggtgt
    16381 ataaattcct gagtgccaaa aatagaacgt ccagccatgt tttataccat gttatggcga
    16441 atggtgatgc cgacatgctg aaaatttttt tggtcgcgtt acctttgtta attcgcacat
    16501 gtcatttgac taaagaacag gtactcgatc tcctgaaggc aaaggatttt tatggttgcc
    16561 caagactata cctggcgatg caaaatggac atagcgatat cgtgaaagtt attctcgaag
    16621 cattgccctg tctggcccag gaaattaaca tttcagcttc cgatattgta gatcttctga
    16681 ccgctaaaag tcttgcgcgc gacacgggtt tgtttatggc catgcagcgc ggacatatga
    16741 acgttattaa tactattttt aacgcattac ccactctttt taatacgttt aaattcgata
    16801 aaaaaatatg aagcccctcc tcctggcaaa taattctaat gaatatcccg gtttgttttc
    16861 agcgatacag cataaacaac aaaacgttgt agagacggtt tatcttgctt tatctgacca
    16921 tgcacgcctg tttggattta ccgctgaaga tattatggat ttttggcaac acaaagcgcc
    16981 acaaaaatac tctgcctttg agttggcttt tgaatttggt caccgggtta ttgctgaatt
    17041 aatccttaat acattaaata agatggctga aagctttggc tttacggata accctcgata
    17101 cattgcggag aaaaattata tggaagcttt actcaaaaaa gcatctcccc ataccgtacg
    17161 ctaaatcttt gcctgcgccg atacgcataa acatcttcca catgcccttc acgtatgcgg
    17221 ttttgtagtg cgcgccagta atcagcgcgg aacaggtcgg cgtgcatctc ttcaaacagc
    17281 ggaccaatac gcgggtcggc gcatagccag tggcgaaact cttccgggaa aacatcgccc
    17341 ggcgagacgc tgtaccacgg ttcgctggca agttcgtctt ccggatagcg cggcggcggg
    17401 atgtcgcgga aattcacttc cgtcatgtag caaatttcat cgtaatcata aaaaaccaca
    17461 cgcccgtgac gggtgacacc gaagttttta aacagcatgt cgccagggaa aatgttagca
    17521 gcggcaagct ggcgaatagc gttaccgtat tcttcaatgg cgtcgcgcaa ctgctgacct
    17581 tccacttgtt ccagccagat attgagcggc accatccgcc gctcgattat agagatggcg
    17641 aattacaatc tgctcaccaa gatcggtgat tttttccgct gcttcctgga gcagtaattc
    17701 cattaatacc ggggaaatat gccgcttctc cagcacaaag ttttcaaact cctgggtgtc
    17761 cgccattcgg cccacacggt cgtgctcttt caccagttga tagcaggcac gaacgtgagc
    17821 ggcagacatc tctttctgcg gcgcgaactt gtctttgatg actttgaata cccgatcaaa
    17881 gcccggcagc gtaaacacca acatcaccat tccacgaata cccggcgctt caatgaactg
    17941 ctcattacag ccctgtagat aaacgagata ttcgcggtag ctttcggttt tggcgtgctt
    18001 ctggcagccg atagccatat acaattcagc agtggtttta cctggcagaa tttcccgtag
    18061 ccactcgacc agcgctgcgg gcagcggtgc ataaaccata aaataagaac gcgcaaagcc
    18121 aaaaacaatg ctcgcttcgg cggtcgtcgt caggcaggta tcaataaata actcgccgtc
    18181 gtccgtctgg tggatcggca gtaagaaggg caacgtgccg gaaggcgtga tcagtttgcc
    18241 caccagccag gcggctttat tgcggtaaaa cagttcgttc gccacctgta aatgactttc
    18301 cgcgaggttg tctgtcccca gcgtttccgt cagatggcga atgatgtaat gaatgtcacg
    18361 gcttttattc tgccagcgca ggcgcagcgg caggtcgctg ataacgcgca tcagtagaga
    18421 ttcccagccg tgatcggggt gaaagtcttt cgccagcggg cggggaatgg tacgaaagcg
    18481 gcgctctggc tgagagctaa agataaaaag ccgctcggga gtaagcgagc ggtggtcaaa
    18541 taaccgacag tacacggagt taaaaaagct ctccgcaatc tcgaagcgcg ggtaatccgg
    18601 caacagccgc gtgtaatgct ctttaacgcg cagtaaaaat gccgcgtccg tactttggcc
    18661 gttagtaatg cagcgcagtt gctccacgac cagaccaacg tgatgatcgt aaagatggat
    18721 acggtttttc atcgcctgct ggacagcatg ccagtcggcc tgttcgaaac gctgctgcgc
    18781 cccggaagtc acttcgagga atcgaccata ctgagcgtcg aagccttgca aaatggtttg
    18841 agcaatcagt aattccaggc cacgcggcat ttagcgccct catcaggagc agagaattgc
    18901 cggatgcggc gtaaacgcct tatccagcct acgttcggca acggttgtag gcatgataag
    18961 acgcgcaagc gtcgcatcag gcatcgtgca ccgattgccg gatgcggcgt aaatgcctta
    19021 tccggcctac agtcagcaac gtttgtaggt tgcttagaac tgcgattctt cagtggagcc
    19081 agtcagcgcg gtgactgaag acgtgccgcc ctgaataata gtcgtcactt tatcgaagta
    19141 acctgtaccc acttcctgct ggtgagatac gaaggtatag ccatctttcg cggcggcaaa
    19201 ttccggctgc tgcactttct caacatagtg cttcatacct tcgccctgcg cataagcgtt
    19261 tgccaggtcg aacatgttaa accacatgct gtggatacct gctagggtga tgaactggaa
    19321 cttgtagccc atatccgaca gctgctgctg gaagctggca atagttttgt cgtcgaggtt
    19381 tttctgccag ttgaacgacg gcgagcagtt ataagccagc agtttgcccg gatatttcgc
    19441 gtggatagct tgtgcaaagc gacgcgccag ttccagatcc ggcgtggagg tttcacacca
    19501 gaccaggtca gcatatggcg catacgccag gccacggctg atcgcttgct caatgcccgc
    19561 atgagtacgg aagaagcctt cactggtacg ctcgccggta ataaattcgc tgtcatacgg
    19621 gtcgcaatct gaggtgatca gatccgccgc atcagcatcg gtacgtgcaa ccagcagggt
    19681 tggaacgccc gtcacgtcag ctgccagacg cgccgcgacc agtttctgaa tagcttcctg
    19741 agttggcact aaaactttgc cgcccatgtg accgcatttc ttcactgacg ccagctgatc
    19801 ttcgaagtga actgccgcag caccggcttc aatcatcgct ttcatcagtt caaaggcatt
    19861 caggacaccg ccaaaaccgg cttccgcatc ggcaacgatc ggcaggaagt aatcgacata
    19921 gcgcggatcg cccggctcaa tgcccacgga ccattggatc tgatcggcac gacggaaggt
    19981 gttgttgatc cgctccacca cagctggcac cgagtttgcc ggatagagcg actgatccgg
    20041 atacatgctg gccgccaggt tagcgtccgc cgctacctgc catcccgaca gatagactgc
    20101 ttcaataccc gctttcgcct gttgcagcgc ctgaccgcca gtcagtgcac cgaggttgtt
    20161 gatgtagcct tttttcgact caccgtgcag cagacgccac attttcgctg cgcccagttg
    20221 cgccagcgtg cattcaggat tgactgaacc gcgtaatttc accacatctt ccgcactgta
    20281 tgggcgagta atgccttccc aacgcggttg agtccactct ttctgtaatt cttcaatttg
    20341 ttgtgtacgg gttttcatgt gcagatgctc catagttatg tggtggttta cgctaacagg
    20401 cggtagcctg gcagggtcag gaaatcaatt aactcatcgg aagtggtgat ctgttccatc
    20461 aagcgtgcgg catcgtcaaa acggccctgg gagaaacgtt cttcgcccag ttcgctggca
    20521 atgactttca tctcttcgcc cagcatctgg cggaacaagg ctttggtcac cggtttgcca
    20581 ttgctcaacg ttttttgatg atggatccac tgccagatcg aggtacggga aatttcagcc
    20641 gtcgccgcat cttccatcag gccataaatc ggcacgcagc cgttgccgga gatccacgct
    20701 tcgatgtact gcacagccac gcgaatgtta gcgcgcatac cttcttcggt gcgttcacca
    20761 tcacaaggtg ccagcagctg atcggcagta atcggcgcgt cttgttcgcg caatacttca
    20821 agctgatttt tacgggagcc gagaatgtcg ttgaataccg ccattgccgt gtccgcaagg
    20881 cctgggtgag cgatccatgt gccatcgtga ccgttattgg cttccagcgc tttatccgct
    20941 tttactttgt tgagcacctg gttattacgc tcttcatctt tgctcggaat aaacgcggcc
    21001 atgccgccca tcgcaaaagc accgcgttta tggcaggttt taatcagcag gcgtgagtaa
    21061 gcattcagga atggtttatc catcgtcacg gcctgtctgt ctggcaggac acgatcggga
    21121 tagtttttca acgttttgat atagctgaag atgtaatccc agcgaccgca gttcagacca
    21181 acaatatggt cgcgcagcgc gtgaaggatt tcgtccatct ggaacacggc gggcagcgtt
    21241 tcaatcagca acgtcgcctt gatggtgccg cgcggcagat taaagcgatc ttctgcatag
    21301 ctgaagactt cgctccacca ggccgcttcc tgctaggact gggttttcgg cagatagaaa
    21361 tagggaccgc tgccctttgc caacagtgcc tgatagttgt ggaagaaata gagcgcaaaa
    21421 tcaaacaggc taccggggat tgcctcacca cgccaggtga catgtttttc cggcaagtgc
    21481 agaccgcgta cccgacaaat caaaaccgct ggattgggct tgagctgata aattttgcct
    21541 gcttcattgg tatagctgat ggtaccgtta accgcatcgc gcaggttaat ttgcccgtcg
    21601 atcactttgt tccagtctgg tgccagtgaa tcttcgaaat cggccataaa gactttcaca
    21661 ttggcgttca gtgcgttgat caccatcttg cgctctaccg ggccagttat ctctacgcgg
    21721 cggtcttcta agtccgcagg aatcccgcga attttccaat cagcatcgcg aatggaagcc
    21781 gtttccgaaa taaaatcagg caacgttccg ttatcaatat cttgctgctg ctgaatgcgc
    21841 gctgccagaa gtttattgcg ttgtggcgta aaatgcgtca ccagctcagt cagaaattct
    21901 accgcttcgg cagtaagaat ttgcttctcc tgctcgccat acggccttgt gaaagccagt
    21961 tcatcggttg ttgttgcctg ttcagtcatc gtgcagctcc tcgtcatgga tccgaaaact
    22021 tcccctctgt gaacgaagga acgttgtgcg cttttcgttc agcacaacta agactactca
    22081 tttaaaatgc aaaatcaaaa acaatttcca ttttaattaa aaatacttga taacttattg
    22141 ataacaatat gaataaaatt tattcacttg ctgaggtgcg tttcggaaat aaaaaaggca
    22201 cccgaaggtg cctgaggtaa ggtgctgaat cgcttaacga tcgactatca cagaagatta
    22261 atccagcgtt ggattcatgt gccgtagatc gtatggcgtg atctgataga cgtaatagtt
    22321 gagccagttg gtaaacagca aattaccgtg actacgccag ctcgctcgcg gtttgttttg
    22381 cggatcgttg tgcgggaaat agttatacgg tacatccggg tctagtccgg cttccacatc
    22441 gcgtaaatat tcctgcgcca gcgtttgcgc atcatattcg ggatggcccg tcacaaaggc
    22501 aatgcgctta tctttgctgg caaacagata tgcatcccct tcttccgtct ctgccagaat
    22561 ttccagatcg gtgtaatcac gaatcaacgc tgccggaaag tcagcatagc gcgaatgcgg
    22621 tgccaggaat gaatcatcaa agccacgcgt cagaagcgca tgaggatgga gaatatgatg
    22681 ctcgtaaacg ccagagagtt tgtcggtgcg agtttgctta ggaatgccgt agaggatatt
    22741 gagcgcggcc tgtaccgccc agcagacaaa cagcgtcgag gtgacgtgat ctttcgacca
    22801 ctccagcacc tgtttgatct gcggccagta agcgacatca ttaaactcca ccaggcccag
    22861 cggcgcaccg gttacaatca gaccgtcaaa gttctgttcc tgaatatctt caaagttaca
    22921 gtagaagttg ttcagatgct ctgcgggcgt gttgcgcgat tcacgggaat cgatgcgcaa
    22981 cagctgaata tcgacctgca aaggtgagtt tgaaagcagg cgcagaaact gattttcagt
    23041 ttcaatcttc ttcggcatca ggttaaggat cagaacctta agtggacgaa tttcctgacc
    23101 agacgcacga gaagttgtca tcacaaagac gttttcttca cgcaagaaat tgacggcggg
    23161 tagctcgtcc ggcacacgaa tcggcataac ctgattacct cactacatac gcttatacgt
    23221 ttagacatcc agatagctga agataaccag aaaatttgcc aatgtcgagc ctgcatgttg
    23281 aaggtgagaa agtttcaagg aaaaggctac gttagaatat aagaatgacg aaaaggagag
    23341 aaaatggtta ttagtattcg ccgctcacgg catgaggaag gggaggaact cgttgcgatt
    23401 tggtgtcgat ctgtcgatgc cacccacgat tttttatcag cagagtatcg ggccgagctg
    23461 gaagagctgg tgcgttcctt tctgccggaa gcaccgttgt gggtcgcggt taatgaacgg
    23521 gagcagccgg ttgggtttat gttgttaagt gggcagcata tggatgcgct gtttatcgat
    23581 cctgatgtgt gcggctgcgg cgtgggtcgg atgctggtgg agcatgcgct ctcgatggca
    23641 ccggaactga caaccaacgt taatgagcaa aatgagcagg cggttgggtt ctataagaag
    23701 gtgggtttta aggttacggg acgctctgag gtggacgatt tggggaaacc gtatccgttg
    23761 ctgaatctgg cgtatgtggg ggaataagcc gcctctgccg tgcctgccca agctggagaa
    23821 gacg
//
LOCUS       DAJTUI010000061        22610 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTUI010000061 DAJTUI010000000
VERSION     DAJTUI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.061"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..526
                     /locus_tag="OO464_002562"
     CDS             <1..526
                     /locus_tag="OO464_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HCR6227666.1"
                     /translation="NNMEFANYGPFELLQARQLIESNIAEFAATQVTKQDIMKLMAIQ
                     EQARGEQCFRDSEWDLQFHIQVALATQNSALAAIVEKMWTQRSHNPYWKKLHEHIDSR
                     TVDNWCDDHDQILKALIRKDPHAAKLAMWQHLENTKIMLFNETSDDFEFNADRYLFAE
                     NPVVHLDTATSGSK"
     gene            871..1533
                     /locus_tag="OO464_002563"
     CDS             871..1533
                     /locus_tag="OO464_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6227667.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1537..1920
                     /gene="mzrA"
                     /locus_tag="OO464_002564"
     CDS             1537..1920
                     /gene="mzrA"
                     /locus_tag="OO464_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6227668.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2067..2421
                     /locus_tag="OO464_002565"
                     /pseudo
     CDS             2067..2421
                     /locus_tag="OO464_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2459..2764
                     /locus_tag="OO464_002566"
     CDS             2459..2764
                     /locus_tag="OO464_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6227669.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            2767..3171
                     /locus_tag="OO464_002567"
     CDS             2767..3171
                     /locus_tag="OO464_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6227670.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3161..3460
                     /locus_tag="OO464_002568"
     CDS             3161..3460
                     /locus_tag="OO464_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6227671.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3646..3942
                     /locus_tag="OO464_002569"
     CDS             3646..3942
                     /locus_tag="OO464_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6227672.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4012..4998
                     /gene="yqjG"
                     /locus_tag="OO464_002570"
     CDS             4012..4998
                     /gene="yqjG"
                     /locus_tag="OO464_002570"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR6227673.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5291..5659
                     /locus_tag="OO464_002571"
     CDS             5291..5659
                     /locus_tag="OO464_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6227674.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(5866..6762)
                     /gene="yhaJ"
                     /locus_tag="OO464_002572"
     CDS             complement(5866..6762)
                     /gene="yhaJ"
                     /locus_tag="OO464_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR6227675.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            6867..7568
                     /locus_tag="OO464_002573"
     CDS             6867..7568
                     /locus_tag="OO464_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6227676.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7591..7755
                     /locus_tag="OO464_002574"
     CDS             7591..7755
                     /locus_tag="OO464_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227677.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(7967..9277)
                     /locus_tag="OO464_002575"
     CDS             complement(7967..9277)
                     /locus_tag="OO464_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6227678.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9305..10636)
                     /locus_tag="OO464_002576"
     CDS             complement(9305..10636)
                     /locus_tag="OO464_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6227679.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(10911..12275)
                     /gene="tdcG"
                     /locus_tag="OO464_002577"
     CDS             complement(10911..12275)
                     /gene="tdcG"
                     /locus_tag="OO464_002577"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6227680.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12347..12736)
                     /locus_tag="OO464_002578"
     CDS             complement(12347..12736)
                     /locus_tag="OO464_002578"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6227681.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(12750..15044)
                     /gene="tdcE"
                     /locus_tag="OO464_002579"
     CDS             complement(12750..15044)
                     /gene="tdcE"
                     /locus_tag="OO464_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR6227682.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15078..16286)
                     /gene="tdcD"
                     /locus_tag="OO464_002580"
     CDS             complement(15078..16286)
                     /gene="tdcD"
                     /locus_tag="OO464_002580"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6227683.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16312..17643)
                     /gene="tdcC"
                     /locus_tag="OO464_002581"
     CDS             complement(16312..17643)
                     /gene="tdcC"
                     /locus_tag="OO464_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6227684.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(17665..18654)
                     /gene="tdcB"
                     /locus_tag="OO464_002582"
     CDS             complement(17665..18654)
                     /gene="tdcB"
                     /locus_tag="OO464_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6227685.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(18753..19691)
                     /gene="tdcA"
                     /locus_tag="OO464_002583"
     CDS             complement(18753..19691)
                     /gene="tdcA"
                     /locus_tag="OO464_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6227686.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            19881..20225
                     /gene="tdcR"
                     /locus_tag="OO464_002584"
     CDS             19881..20225
                     /gene="tdcR"
                     /locus_tag="OO464_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6227687.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20481..21021
                     /locus_tag="OO464_002585"
                     /pseudo
     CDS             20481..21021
                     /locus_tag="OO464_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21043..21390
                     /locus_tag="OO464_002586"
     CDS             21043..21390
                     /locus_tag="OO464_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227688.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21559..22209
                     /locus_tag="OO464_002587"
     CDS             21559..22209
                     /locus_tag="OO464_002587"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227689.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         6098 a         5707 c         5203 g         5602 t
ORIGIN      
        1 caataatatg gaattcgcca attacggtcc gtttgagttg cttcaggctc gccagctcat
       61 cgaaagtaat attgccgagt tcgcggcaac tcaggtaacg aaacaagaca tcatgaaact
      121 gatggccatt caggaacagg cgcgcggcga acaatgcttc cgtgattccg agtgggattt
      181 acagttccac attcaggtag ccctggcgac gcagaactcc gccctggcgg ccatcgttga
      241 aaaaatgtgg acccagcgta gccataaccc gtactggaaa aaactacacg aacacattga
      301 ttcccgtacc gttgataact ggtgtgatga ccacgatcaa attctcaagg cgttaattcg
      361 caaagatcct catgctgcta agctggcaat gtggcagcat ctggaaaaca ccaaaatcat
      421 gttatttaac gaaaccagcg acgatttcga gttcaatgcc gaccgctatc tgttcgccga
      481 aaacccggta gtgcatctcg atacggccac cagcggcagc aaatgaaatt cctgtccgac
      541 aggcgctgca atgcgcctgt ttgtctcgtc ggtaagcgaa aagtataaag tgtcagcctg
      601 tgtaaatcct ctcgccaccc tcccctgcaa tcagcaaaat cagactccac ggacatgtaa
      661 ttttgataac gaacaacgtt gacctttgtt acaattagat tcaatttgaa tttatgcttt
      721 tgaatgcttt cttatctcac gttttaacag ggaatagttc aggctgtgtt gatgtatcaa
      781 acccgcagaa cataccaaaa cagcaataac attgcggtag tgcatctttt aaaaccagcg
      841 tggcgttaac cgattcacca ggaataatga atggaacttt tgacccaatt gctgcaagcc
      901 ctgtgggcgc aggattttga aaccctggcc aatccatcga tgattggcat gttgtatttt
      961 gtcttgtttg taattttgtt ccttgaaaat ggcttgcttc cggcggcctt tttaccgggc
     1021 gacagtttac tggtattggt cggcgtgttg attgcgaaag gcgcgatggg ttacccgcaa
     1081 acgattctgc tgctgaccgt tgccgccagc ctcggttgct gggtcagcta tattcagggg
     1141 cgatggctgg gcaatacccg caccgtacaa aactggctat ctcatttacc cgcgcattat
     1201 catcaacgcg cacaccatct ttttcataaa cacggtttat cggcgctgtt aattggtcgc
     1261 tttattgcat ttgtcagaac actgctgccg acgattgccg ggttatcagg gctgaataac
     1321 gcgcgctttc agttcttcaa ctggatgagc ggtctgctgt gggtattgat cctgacaact
     1381 ctgggttaca tgctcggcaa aacgccggta tttttaaagt acgaggacca gctgatgtca
     1441 tgcctgatgc tgctcccggt ggtgctgctg gtgtttggcc tggcaggttc tctggtcgtg
     1501 ttatggaaaa agaaatatgg aaatcggggg taagggatgc aaatacctcg catgtcgctt
     1561 cgccagctag cctggtccgg cactgtttta cttctggtcg gcacgctgtt gctggcctgg
     1621 tccgcggttc gccagcaaga gtctacgctg gcgattcgtg ccgttcatca aggcacaacg
     1681 atgccagacg gtttttcgat ctggcatcac cttgacgctc atggcattcc tttcaaaagt
     1741 atcaccccca aaaacgacac tctgttaatt acatttgact ccagcgacca gagcgccgcc
     1801 gcaaaagcgg tcctcgacag aacattgccc catggctaca tcattgcgca gcaggacaat
     1861 aacagtcagg ctatgcagtg gctgacccgg ttacgggata actctcatcg cttcggataa
     1921 cttcctgtta tccgaaacat atcactcact ttggtgattt caccgtaact gtctatgatt
     1981 aatgaggcgg tggttaacac taccgcttcg ttgtctggac aatcgttcct ttgtaatagg
     2041 tccgaatcac aatggaaggt tcaagaatga aataccgcat cgctttagct gtttctctct
     2101 ttgctcttag tgccggtagt tatgccacta ccctgtgtca ggaaaaggag caaaatatcc
     2161 ttaaggagat cagctatgcc gaaaaacacc aaaaccagaa tcgtattgac ggtctgaata
     2221 aagccctgag tgaagtccgg gccaactgtt cagatagcca gctgcgtgcc gatcatcaca
     2281 agaaaatcgc aaagcagaaa gatgaggtgg cggaacgcca gcaagattta gccgaggcga
     2341 agcaaaaagg cgatgccgat agaacggaaa ctggcagaag cgcaggaaga gctgaaaaag
     2401 ctggaagcgc gcgactacta actcacaata gtcactactt actcacctgg agaaaactat
     2461 gtcgaaagaa cacactacgg aacatctgcg tgctgagttg aaatcccttt ccgatacgct
     2521 ggaagaggtg cttagctcat ctggcgagaa gtcgaaagaa gagttgagta agattcgtag
     2581 caaagcggag caggcactga aacagagccg ttatcgcctg ggtgaaaccg gtgatgccat
     2641 cgccaaacaa acccgtgtcg cggcggcgcg tgctgatgag tatgtgcgtg aaaatccgtg
     2701 gaccggcgtg ggaattggcg ctgcaatcgg tgtagtgctc ggcgttctgc tgtcgcgtcg
     2761 ttaattatgg cggacactca tcacgcgcaa gggcccggta aaagcgttct gggcatcggg
     2821 cagcgaattg tttccatcat ggttgaaatg gtggaaacac gtctgcggct ggcggtggtg
     2881 gagctggaag aggaaaaagc taatctcttt caacttttac tgatgctggg cctgacgatg
     2941 cttttcgctg catttggtct tatgagcctg atggtgctaa ttatttgggc ggttgatccg
     3001 caatatcgcc tgaatgcgat gattgccacc accgtggtgt tgctgctact ggcactgatt
     3061 ggcggtatct ggacgttacg taaatcgcgt aaatccacct tgctgcgcca tacacgccat
     3121 gagctggcaa acgatcggca actgctcgag gaggagtccc gtgagcagta aagtcgaacg
     3181 tgaacgacgt aaggcgcaac tgcttagcca gatccagcaa caacggctgg atatttccgc
     3241 cagtagtcgt gaatggctgg aggcaacagg cgcttacgat cgtcgctgga atatgctgct
     3301 aagtctgcgc tcctgggcgc tggttggcag tagagtgatg gcgatctgga cgattcgcca
     3361 tcctaatatg ctggtccgct gggccagacg cggttttggc gtatggagcg cctggcgtct
     3421 ggttaaaacg accctcaagc agcaacagct tcgcggttaa cttccctctg gccggagcaa
     3481 ttccggcctt atccatcaaa ttttttgaat atttttgaca gttttccttg ctaacaatca
     3541 tcattcacca cgtttatgat tctctccatc gacagcaacg acgctaatag cgcgcccttg
     3601 cacaaaaaaa caatcagcag cctgagtggc gcagtggaga gtatgatgaa aaaattagaa
     3661 gatgttggtg tactggtagc gcacatttta atgccgattc tgtttattac cgcaggctgg
     3721 ggaaaaatca ccgcttactc tggtacccaa caatatatgg aagcaatggg tgtcccgggt
     3781 tttatgctgc tgacggcatt tttatttcac agcaactttg ctgaaggcgt caactcgctg
     3841 atgttcatga aaaacctgac aatttctggc ggattcctgc tgctggcaat taccggtccg
     3901 ggcgcgtata gcatcggccg cctgctgaat aaaaagtggt aagcacgcgc tatacttaac
     3961 gataaaaaac gaggaggaag ctcctcgttt ttgctattgg aggagagaaa aatgggtcaa
     4021 ctgattgacg gcgtctggca tgacacctgg tacgatacca aatctaccgg cggtaaattt
     4081 caacgttcag cttccgcatt tcgtaactgg ctcactgccg atggcgcacc tggccccact
     4141 ggcacaggcg gttttatcgc agagaaagat cgttatcatc tctatgtttc actcgcctgc
     4201 ccgtgggcgc accgcacgct gatcatgcgc aaactcaaag gactggaacc gtttatttcc
     4261 gtttccgtag tgaacccgct gatgctggaa aacggctgga cctttgatga cagttttccg
     4321 ggagcaaccg gagatacgct ctatcaacat gaatttctgt atcagcttta tctccacgcc
     4381 gatccacact acagcggacg agttactgtt cccgtgctgt gggacaaaaa gaaccacacc
     4441 atcgtcagca acgaatcagc agaaatcatc cgcatgttca ataccgcgtt tgatgcgctg
     4501 ggcgcaaaag cgggtgatta ctacccacca gccctgcaaa cgaaaattga cgaacttaac
     4561 ggctggattt atgacaccgt taacaacggc gtgtataaag ctggttttgc caccagccag
     4621 caagcctacg acgaggcggt ggcgaaagtg tttgaatcgc tggcgcgact ggagcagatt
     4681 ttaggccagc atcgttacct gaccggcaac cagctaaccg aagccgatat tcgcctgtgg
     4741 actacgctgg tgcgttttga tccagtgtat gtgacccact tcaagtgtga taagcaccgc
     4801 atcagcgatt acctgaatct gtatggcttc ctgcgcgata tctaccagat gccggggatc
     4861 gccgaaactg tcaatttcga tcacatccgt aatcattact tccgcagcca taagaccatc
     4921 aaccctacgg ggattatttc aattggtccg tggcaggatc tcgatgaacc gcatggacga
     4981 gatgttcgct tcggttaaaa aaaggcacct tacgaggtgc ctctgataat tagctaagtg
     5041 catgttgttc atgtcggatg cggcgtgaac gccttatcaa acctacaaag cgtttatatt
     5101 caatatgtta caatgaccgc gtaggccaga taagcgtagc gcatctggcg attttgcgct
     5161 tgccatcagt cttagcgcgc cttcacggca ctttattaat tccttgaacg aatatttact
     5221 gccatttcat tcacgtctat tcttaatttg ctgctttaaa aacaagtgat tgagcaaaat
     5281 tgaggcaaaa atggactggt atctgaaagt actaaaaaat tatgtcggtt tccggggtcg
     5341 tgcgcggcgc aaagagtact ggatgtttat tctggtcaac atcatcttta cgttcgtgct
     5401 ggggctgctg gataaaatgt taggctggca acgcgctggt ggcgaaggca tcctgacgat
     5461 aatctacggt attctggtgt ttttaccgtg gtgggcggtt cagttccgcc gcctgcacga
     5521 caccgaccgc tcggcgtggt gggcactgct attcttaatc ccgtttatcg gctggcttat
     5581 tatcatcgtc tttaactgcc aggcgggtac gccaggcgaa aaccgctttg gaccagatcc
     5641 aaaactggaa caagaataat gattaagcac cttgtaaaag gtgcttcaaa ttgttgacaa
     5701 agtgcgcttt gtttatgccg gatgcggcgt aaacgcctta tccagcctac aaattgccaa
     5761 aattcaatat attgcagtga ctgcgtaggc ctgataagcg tagcgcatca ggcagttttg
     5821 cgtttgtcag cagtcagaag caccttgtga aaggtgctta atagattatt ttccagcgaa
     5881 caactttgga atttcccgca gacaccagga tttcgcttcc cccatactgt cacgacgcca
     5941 ggccataata atatcgatct cgctggtcga ttccgggctg acgacacgca accgcccttc
     6001 cgcaatatct ttttcgacca tcggatacgg catcgtcgcc acgccaagcc ccgccagtaa
     6061 tgcctgacgt ttatcttcaa tcgtgctcac cgttaagcgc ggctgtttgt ccagcagctg
     6121 tacggtcaac accgggcgct cacgagcggt atccgctacc gcaattccac gatatttcac
     6181 acgcgtcact tcagataacg gttccggctc ctgatgaatc gggtgatcag gcgcggcaac
     6241 gtagacgttc attaacgtat agagcttgcg cgagttgatc tccgacgagg aacgaaaatg
     6301 catatccggc gcgataacga tatccgcccg cccctgctcc agccgttccc acgcccccgc
     6361 cagcacttct gtgatgattg ccagttgggt attggctttt gccgccagtt tgtcgattaa
     6421 cgggaaaaag gcaggtgtcg gtaccagcgc ttcggtcaca atggtgagat gcgtttccca
     6481 accgcgcgcg agagcttccg catcagtagt cagtttatct gcggcttcca gcaaaacgcg
     6541 ccctcgctcc agcaacatcc tcccgacatt ggtgaatttg gtgcgatggc ccgagcggtc
     6601 aaacagcacc acatccagct cttcttccag tttttgcatg gtgtagctaa gtgcggaagg
     6661 cacgcgtccc agctcatccg ctgccgccgc aaaactgccc cggcgatcga tcgcatccat
     6721 aacccgtagt gcttccagcg ttaatgccct ttctttggcc atttcgttct cattcaggaa
     6781 atttgaacat accgggcaga atatctggct aacaatgtag cgtccagccc cttaccataa
     6841 aaggaagtaa agagaggtca agaattatga ttactacccg aactgccagg cagtgtggac
     6901 aagcagacta cggatggttg caggcccggt atactttttc ctttggacac tacttcgacc
     6961 cgaaattgtt aggctatgcc tccctgcgtg tgcttaacca ggaagtgctg gccccaggtg
     7021 ccgcctttca gccgcgaacc tatcccaaag tcgatatttt aaatgtgatt ctggatggag
     7081 aagcagagta tcgcgacagc gaaggcaatc atgttcaggc cagcgccgga gaggcgttgc
     7141 tgctctctac ccagccgggt gtcagctata gcgaacacaa tctcagcaaa gacaaaccgt
     7201 taacgcgaat gcagctttgg ctggatacct gcccgcagcg agagaatccg ctgattcaaa
     7261 agctggcgct taatatggac aagcagcaat taatcgcctc gccagagggt gcgatgggaa
     7321 gcctgcaatt acgccaacaa gtgtggctgc accatatcgt gctcgacaaa ggcgaaagtg
     7381 cgaatttcca gttgcatggg ccacgcgcgt atttgcaatc gattcacggg aaatttcatg
     7441 cgcttacgca tcatgaagag aaagcggcgc taacctgcgg tgatggggcg tttattcgtg
     7501 acgaggctaa cattacgctg gttgccgatt ccccactgcg cgctttgctg atagatttgc
     7561 ctgtctagtt gtttttacag ggagatgatg atgagtaaaa aatcgaccaa aaagcgccag
     7621 ccagcgaagc ccgtggtggc gaaagaacct gctcgcaccg ccaaaaattt tggctatgaa
     7681 gagatgttga gcgagctgga agctatcgtc gcggatgctg aaacgcgttt agccgaggat
     7741 gaagctaccg cgtaaagaca ggttgccgga tgcggcgtaa acgccttatc cggcctacaa
     7801 aacacaacaa catcaacaaa ttaaacaccc cgtaggccgg ataagatgcg ccagcatcgc
     7861 atccggcatc aggtacaaaa cacaacaaca tcaacaagtt gagcaccccg taggccggat
     7921 aaggtgcgcc agcatcgcat ccggcacaat actcaaaacc tggcgtttat ctggccttgc
     7981 tcgccataat ctcgataatc tgccgatccg tttgctgcat cgaatggctt gctaacgcac
     8041 acaggttggc aatcgactgc tcaacatcat gcgccacgat cccttcattg ccggtcacgg
     8101 cggtatcatc cagcgccatt aacaccgctt tccacgcagc cgaagcactg gtcgaaacct
     8161 tcatcgcgca gctgttcgac gcaccatcgc aaatcatgcc gctgacatcg ccgatcatac
     8221 tgctgatcgc catcgagata gtttcataac gcccatccac cagccatgcc atcccggcgg
     8281 cggcccccat tgctgcggtc gttgcggcac aaagtgcaga caaacgcggt aactggttat
     8341 ggatgtaaat tgcgctcaaa tgagaaagca tcagcgcacg cgccagtcgt tcatcatccg
     8401 ctccgaagtg ttctgctaca accaccacag gcattattgc agtgatcccc tggttacccg
     8461 agccggagtt actcatagcc ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc
     8521 tggtacgaat cacaatggat gaagagagat ctttcgccag caaaccgcgc tcgcactgtt
     8581 tttccagcgt cgcgccaata tgcagccccc acttaccgct caaaccttcc tgcgataacg
     8641 cacaatttag cttcgcggaa tcgagaataa agcggatcgc cgcaaatggg acttcattga
     8701 cgaacttcag gatctcagcc agcgtcgttc tggaaagcac cgtcagcggc gactcttgct
     8761 cgccctctgc cacacacgcc tggtgggtaa acaccacacc attgtgcgtc tcaatatgca
     8821 caatgttggt atgcccgccg acgatggtga cacacgccca cttctcaccg ttccagactt
     8881 tggcgcgtga gaagaggatt tcattgcaag gttcctggat cttaacagag actttccccg
     8941 ccgccagcag tgctttggca tcggtaattg cctgcgcagt tgcgtctttc agcacttcca
     9001 gcccggcgtt ggcatttcca cctaacgccc ccagcgccgc cgcaatcggc agccccacca
     9061 ttcccgtgcc gggaacggtg acgcccagac cgttcttcat cagatttggc gaaacccagg
     9121 cttctacacg ttcaaccgga ccttccagtt ctgccgcagc aaccgccgcc gccagcgcca
     9181 gtgaaatcgg ttcagtacat cccagcgccg gttttacttc ctcctgaacg gcgaggatgt
     9241 aacgctgcca taacggattt aaagtcgaat caaacatatt aaaaacctta aaatttcagg
     9301 taaatcaaga aaatgccagg aacagagaaa cacaaagcaa caaaccagtg acgataatca
     9361 ggtacagaga catccctttg tatttgtgca atgccggtac tttgtaaacc agccacgccg
     9421 ggatcaggca ccctaccatg ccgaaaatcg ggctacagat agaggtgaag ctcaacaccg
     9481 gtgcgttcag tacgatggcg ctccaggcca gcaaaatggc gaaaatcatg atgccgcgct
     9541 gaacaagatt ttcgttaatc ttctcggcag gcatcttgcg acgcaggatg ttcattacga
     9601 tcccttgcgt tgcttcgcga aagcctaaat agacgccaaa gaatgcagtc atgacagcaa
     9661 agatattgag aatgacgctg accactttca cccacgctgc gccgtcaccg ctaataaact
     9721 gtgcggcaat cgccagcgcg gaaatattct gctcgtaggc ttttaccgct tcgtcatgtc
     9781 ccatcgccag cgtgaacgac acggcgtaga aaaagacggt gacaaacaaa atgccaaacg
     9841 cgatattcat tgcccgcaat gctttatgcc gcgccacttc aattgatttt tcccgcgagc
     9901 gataagagat caccatcggg cttaacgtct ggataaacag aatcgacgtc agggtaaacg
     9961 gcagcgtaat aatggcgttt ttcaccagca gccccagcgg cggtagcgaa ccgacgttat
    10021 acagatgcca cattcccacc atcgacacgc ccagcgcagc gactaccagc agcttggtca
    10081 gcaccatgtc ggtcgaaatt ttgaataaca atttctcgcc gcgtgaagag atcgccacca
    10141 gaatgcaaat cagtaccaga ccataaaagg gactgtctga cagcaaccct tccgtcacgc
    10201 cgaaggtatg cagataggaa gcactatcgt tggtgatggc ggtggaatag acgaacatcc
    10261 aaatcaccag cattacgaaa tagagcgcac ctaacaggat gccccagttt ttacctaaat
    10321 aaccgctaat gacactcggg taatctttac attctggtga ttctgccagc gtattaataa
    10381 acaaccgctg aaacagatac attgccgggt aaccaatcac cgatgagagc aaaaataccc
    10441 acaatcccat caaaccgacc tgcaccggga gaaaaacaat ccccgcgcca atcgccatcc
    10501 cgatactcat aatcacccag ccggtgtcag tactgtcgaa tttgatcgct tctcgccact
    10561 cgctctcact cattccagca cgacccgccg gggtcgaagc gtctgcaatg acgcctttat
    10621 tcgatgcaat ttccataatt tctcgctcaa tattttgtag ggcttattat ttttttccga
    10681 gccgcatcaa ggcgatatgc ggtctacgtt tttgcaggcg agttattaga ataaagaaat
    10741 gatacgcgcg aggtcagaga aaatcttcac aatcaagact ttaaaataac ggagatggat
    10801 aagaattttc tactaaatta atcacaagag aatattcatt ctctatttgc ggcagatcac
    10861 aaaaaaaagg tgcacatttg tgcacccaag gatgaaagct gacagcaatg tcagccgcag
    10921 accactttaa tggccagtcc tccgcgtgat gtttcgcggt atttatcgtt catatctttg
    10981 ccggtttcat acatcgtctc gatcacttta tcgagtgaaa cacgcggtgc cgaggtgcgg
    11041 cgcatcgcca tccgcgcggc gtttactgct ttcacggcat taatggcatt acgttcaatg
    11101 cacgggattt gtacctgtcc ggcaaccgga tcgcaggtca gcccaaggtt atgctccatc
    11161 gcgatttccg ccgcattgca tacctgcgcc ggactgccgc ccagtagttc agttaaccct
    11221 gccgctgcca ttgaacaggc cacgccaatc tccccctgac agccgacctc cgcgccagag
    11281 atggaggcgt tcattttata cagcgcgcca atagcccccg ctgccagaaa atagcgggca
    11341 attgaccgct cgtttaccgg acgacggaac ttatcgtaat aagccagcac tgccggaata
    11401 atgccgcacg caccgttagt cggtgccgtt accacgcgcc cgccagctgc gttttcttca
    11461 ctaaccgcca gcgcgtacat gttgatccag tcgatgacat tcatcggatc gttagagatg
    11521 ttatcgctag aaaccagctg acgacgcagc gctacggcac gacacggcac attgagcgga
    11581 ccaggcagca cgccttcagt gttcatcccg cgttcaatac cgtcatgcat cacttgccag
    11641 atacgggcaa accctgcgtc aatttccgct ttgctgcgca gcgccagctc gttgtgcatc
    11701 atcaggccag atatagacag gccgttgtaa tcacacattt tcagcagttc acctgctgag
    11761 tggaaatcgt aaggtacgga cgtttcgaca tcgtgcgaca ggccgaagtg ttcttcttcg
    11821 acaataaacc cgccgccgac agagtaatag gttttactta atagcgcttc ctgtcccttc
    11881 caggcagtga tccgcattcc gttctcatgg cgaggcaaca tttcgggatg gaagataatg
    11941 ttctttgcta caggaaaatc aacaatatgc gcaccagatg ccactggcag ccgcccgctg
    12001 cgcgttacta actctataaa tgcagggatc tcatcaatga caacatcctg cggactgttt
    12061 cctgccagtc ccatgataat ggcgacatcc gtggcatggc ctttgcccgt cagtgacaac
    12121 gacccgtaca gatcgaccac aatatggctc gtcgcggtta ataagccgct actttccagc
    12181 cgatcaataa aactttttcc ggcattcatt ggccccacgg tatgcgaact ggagggacca
    12241 atcccaattt tgaaaatatc gaatgcacta atcatatcca caccctcgga ttgccgttca
    12301 gtgaagtgga gcggaacgac cttgcgaccg tcccgctcac gaggctttac gcactacgta
    12361 ctgcgatagc ttcaatttcc agcttcacat ctttcggcaa acgcgcaacc tgcacacagc
    12421 tccgggtcgg gtaggtcgcc tgatgctcat cgaagaactg cttatagact tcattgatgg
    12481 tggcaaaatc attcagatcg gtgatgaaca cggtcatctt gatgatatcg cccacgctca
    12541 gcccggcagc aaccacgatc gctttgacgt tttcgaggct taaacgcgcc tgatcttgca
    12601 catcagccgg gatctcaccg gtctgcgggc aaaccggaat ttgcccggag gtgaagacca
    12661 tgctgcctaa atcaacgccc tgaacataag ggccgattgc gcctggggca cgttgcgttt
    12721 cgataatctt tttcatacat cctccggcgt cagagcgcct gggtaaaagt acgtgaaata
    12781 acatcctgtt gctgttcacg ggtcagtgcg ttgaagcgca cggcgtagcc agagacacgg
    12841 attgtcaggt taggatattt ttccgggtgc tcgatggcat ccagcagcat ttcccgattc
    12901 attacgttga cgttgaggtg ttgaccgcct tcgacatccg cttcgtggtg gaaataccca
    12961 tccagcaggc cgacaaggtt ggttttacgt actggatctt ctttgcccag cgccgcagga
    13021 acgattgaga aggtgtacga gatcccatct ttggcgtagg tgaacggcag tttcgccacc
    13081 gacgtcaatg aggccacggc acctttgcgg tcacgaccat gcatcgggtt agcgcccggc
    13141 gcgaacggtg ttccggcgcg acgaccgtcc ggcgtattac cggttttctg gccgtacacc
    13201 acgttagaag tgatagtcag aatcgactgg gtagggacgg cgttgcgata ggttggcagc
    13261 gctttaattt tcttcataaa gcgttcaacc aggtcgcagg caatgctgtc tacgcgctcg
    13321 tcgttgttgc cgtactgcgg atattcaccg tcgatttcaa agtccaccgc caggccgttt
    13381 tcgtcacgga ttggtttcac gcgggcatat ttgatggcag acagggagtc cgtcgccacc
    13441 gacaggcccg cgatgccgca tgccatagtg cgatagacat cacgatcgtg cagcgccatc
    13501 agcgaagctt cgtagctgta cttgtcgtgc atgtagtgaa tgatattcag cgcgctgatg
    13561 tactgcaccg ccagccagtc catgaagtga tcgaggctgt ccatcacttt gtcgtaatcc
    13621 agcacgtcgt ccatcagcgg tgctgttttc ggcccgacct gaatcttcag cttctcgtcc
    13681 accccgccgt taattgcgta gagcagcgtt ttcgccaggt tagcgcgtgc accaaagaac
    13741 tgcatttgct taccaatcac cattgggctg acgcagcagg caatcgcgta atcgtcgctg
    13801 ttgaagtcag tacgcatcag atcgtcattt tcatactgca aggaagaggt gacgatcgac
    13861 acctgcgcgg catatttttt gaaggcaatc ggtaattctt ccgaccaaag aatggtcagg
    13921 ttaggttccg gtgccggccc catagtgtgc agggtgtgca aatagcggaa ggagtttttg
    13981 gtcaccagcg tacgaccgtc cagccccatc ccgccgatca cttccgtcgc ccagattggg
    14041 tcgccggaga acagcgaatc aaattccggt gtacgcagga agcgtaccat acggatcttc
    14101 atgatgaagt gatcgatcag ttcctgtgcc tgctgctcat tgaggacgcc agctttaaag
    14161 tcgcgctcaa tgtagatatc caggaacgat gccgtgcgcc ccagcgacat cgcgccgcca
    14221 ttttgcgatt tcactgccgc cagataagcg aagtagagcc actgcaccgc ttcctgtgca
    14281 ttctgcgccg ggcgagagat atcaaagcca tatttcgccg ccatttcctg aatctgcaac
    14341 agcgcatgac gatgctctgc cagctcctca cgcagacgga tggtggcttc cagatcctcg
    14401 cctttttcca gacgagactg gagatcggca aattgcagtt cgcgttcacg caccagataa
    14461 ctgatgccat acagcgctac gcggcgatag tcaccgataa tgcgcccacg accatagcca
    14521 tctggtaaac cggtcagcac gccagattta cggcagcgca gcatatccgg tgagtaaaca
    14581 tcaaatacgc cctggttatg ggttttacgc agatcggtaa acagatattc aaattcactg
    14641 tccatttcac ggccataggc gtggaatgaa cttttaatca tattaatgcc accgaacggg
    14701 tgtagcgcac gtttcaacgg cgcatccgtt tgcaggccaa caattttttc cagcggctgg
    14761 ttaatatatc ccgcatcatg agcggtaatt gtggtggcaa tattggtatc gaaatcaacc
    14821 ggcgcgtggg ttgcgttttc gatacggatg ccttccatta ccttttccca caattccgtg
    14881 gtggcaggcg tcgcttcggc gagaaaagat tcatcgcctt catacggtgt atagttatgt
    14941 tgaataaaat cgcggacatt aatttcgctt ttccagtccg tacctttaaa gccaagccat
    15001 gcgtcagcgt acagcttatc gctggtatca atatctacct tcatgaaaaa taatctctct
    15061 acaatacttc aactaaatta tgcaaattct gcgggcgcgt taactttacc taaatgaatg
    15121 gcatccaaag caatcatttt ttcttcgtta gtcggaataa cgacgcaaat gacatgcgca
    15181 ttttcactgg aaacaattcg ctcgccaaaa gagttagaac gattattcat ttctgtatca
    15241 atctctacgc ctaatacagc caaatgttcc atgaccagac gacgaattaa acttgaattc
    15301 tctcctattc cgccggtgaa tataattcca tccagccgac gtaatgaagc tgcatgtccg
    15361 gcaatatgac gggcaattcg gtgaacaaag gttttaattg ccagttgcgc gcgttcgtga
    15421 ccttcatgcc aggctttttc cagaacacgt aaatccgaag aaagaccgga aatacctaat
    15481 aatcccgact ctttattcac tacgcgttcc aggtcaccca ggctctggtt ggtttggctg
    15541 gcgacccagg acatcgcacc aaagtcgaca tcgccactgc gggtacccat catcaagcct
    15601 tccagcggcg tcattcccat tgaggtgtca acactctgac cgttgcgaac tgcgcagatt
    15661 gacgcgccat tgccaagatg cgccacaacc aggccggagt catcttccgc cagattcagc
    15721 agcgaatgtg cgcgctggga aacatagcgg tgcgacgtgc cgtggaaacc ataacggcgt
    15781 acacctaact cttcataata tttccacggc aggccgtata aataagcttc cggagccatc
    15841 gtctggtgga aactggtatc aaataccgcc acctgagtta cgcccggaaa taattgctgc
    15901 gccgattcaa taccacttaa atttgcgtaa ttatgcaggg gtgccagtgg agaaacgcga
    15961 cggatattat caatgacttc atcggtaata atggcggact cggtaaaaat actgccgccg
    16021 tgagcgatgc ggtggccaat taaggccacg ctgtcattta aattccgttt ttccagttca
    16081 aatgcaattg ccttcaatgc accttcgtag ctgtggtgag ccagcggtgc tggctctccc
    16141 ccatttacgg ataagaatgc attttccgag ttaataccgt cggcaatacc tgacattaat
    16201 acttcacaat cgctggcatc gagtacggaa aacttaatcg aagacgaacc acagttaata
    16261 accaaaacaa ccggaaattc attcatctct tttctcatcc tgagttacgg attaaaacag
    16321 tttgtatacg atgttcagga tggtcagcag accaatcacg gtaacaaaca cgttatccag
    16381 acgaccacgg tatttcgcca gagacggcgc tttacggata gcatacatcg gcaacaggca
    16441 cagcagggat gcgataatcg gtgcgcccat ggcttcaatc aggtcgagga tgttcgggtt
    16501 ggcgtaggca acaacccagg tggagcccat gatgaagatc atgctgagag tattcagttt
    16561 acccagcgac actttggttt tgtcaccttt ataaccgaac ttcagaatca gaccattcaa
    16621 gccttccagc gtccccagat agtgaccgaa gaaagatttg aagatagcca cgagtgcgat
    16681 gatggaagcc gcatattcca gtgtaatcgc gaacgttgtt ttcgtaccgg tcatggacgc
    16741 aaagtggtta gccagataag aaagcactgg aatattctgc gctttggctt ccgccatgtt
    16801 ggccggagac agagtaaaca ggcagctaaa ggcaaagaac atcaccactg caaccatcag
    16861 catgctggca cgagaaatga tttgggaaca tttacgttcg gtgaagtcgc gaccgaagtc
    16921 tttctcatac tcttcacgtt tagaaaccac gaaggaagag acgattggcg agaagttaaa
    16981 ggagaaaacc atgatggaaa tccccagcca gacagtgatc aggataccgt catgaccggt
    17041 taacgacagc gaaccgaggt caacctggtc gataactgca gagttccagt aagggatcag
    17101 cgacagagaa atcagcacca ggctggcgat aaacggccat accaggtagc tcatcacttt
    17161 aaccatcaga tccttaccaa accagatgac gaaagccatc agcagcaaca ggaacagcgc
    17221 cacaaagccg cgattcagcg gtgcaaatcc gagctggttt tcccagaacg tcataaaggt
    17281 attggtaata gtaacgccat aaatccacag cagtgggcaa atcgcgaaga agtacaggaa
    17341 cgtgataacc acgccgccag ttttaccaaa atgctcttcc accgtttccg taatgttgcc
    17401 ggaagggtta gagccagaaa gacacagacg cgccagcgcc cggtggcaat aaaatgcgat
    17461 ggggtatgcc aataccaaca tcagaagaat cgggatcagt ccgccaaaac ctgcgcggat
    17521 agggaagaac agcaccccgg cgccgattgc cgtaccaaac aagcctaacg tccatgtggt
    17581 atctgattta cgccaggacg attgttttgt ctggctggat acaatgctat ctgaagtact
    17641 catatcctat cctcaacgaa ttaattaagc gtcaacgaaa ccggtgattt gagagacgcg
    17701 agaaagatcg atattgccgc cggaaataat actgacggtt tttctatttt gaatatattg
    17761 gtctaattta ccgcttaata atgcagcaca tgccagagcg cctgcgcctt cggtgacgac
    17821 tttattgcgc tgaattaagg caatcatact gtttctgatt tcgtcttcgc tgaccagcac
    17881 gatgtcatcg actaattcac gaacgatttc gtaagttaaa ttacccgggc gggagacatc
    17941 acaaccatcc gccagggtgc cggtagttcg gtgcgtggtt atttctccgg agtggaaaga
    18001 agccgccatg ccgtgaacgt tttctgactg tacgccaata acacgaatgg tcgggttaat
    18061 agatttaatt gccaccgcaa taccagcaat taaaccacca ccaccaattg gcacaatcac
    18121 gttatcgaca tcatagagat cttccataat ttccagacca atcgttccct ggccagcaat
    18181 cactttcgga tcatcgtaag gtgggataaa aatacgacct tccatttcga caatttcgct
    18241 cactttagcg atagtgtcgt tgaagttatc accatgcaga acgacttctg cggagtagtc
    18301 gcacgttgcc gctactttgg attttggcgc accttttggc atcaccactt taccgtcgat
    18361 acccagcatc gcgcaggaga gcgaaacccc ttgcgcatgg ttgcccgcag aacaggccac
    18421 cacgcctttg cgtttttccg catcggtcag tgaacttaat ttattaaatg cgccacgaat
    18481 tttaaatgaa cccgtacgct gcatattttc aaacttcagg aatatttcac ctttgcaacg
    18541 ttcactaaaa tagttggagc gaggcatgcc tgttttataa attcgcccag ccagtcgttg
    18601 tttcgcttca ataatatcat caatagcaac cggcagatcg tatgtaatat gcattataaa
    18661 acctcttcgc ctgaattaaa tatgtaggta accgtaaccg acacctgcaa aacagacagg
    18721 tggattattt atgtttaaat aaaaacaaac aactaaccaa cttctattaa ttgccttcgt
    18781 ctacacccat tataagatga atactctttg gctaattcca ccaaaaccga tgctgctttt
    18841 ttaatacgat aatttttcga ccataccgcg gcatattgtg ccacaggtaa tgtttcttca
    18901 accggaatag taataaattg attagaacca aaaggtgacg tcatatcaca aggaattaca
    18961 gttaagaaat cagcattgag aacaagatta taaattgtca cgactgagtc ggttttaacg
    19021 atgttttcaa tactgatgcc atttctttgt aacgtagtaa gcagttcgct gtagtacccc
    19081 atattagttt gtggcaacac ccactgttcg ttcttcaacg actccagcgt ggtggtgccg
    19141 gtgcatgttc gggacttact ggctaccagc acaaactcgg actcgaacag cggctcaaca
    19201 tgtaaatcct gaagcttcat ttctgcactt aacgtaccaa tcgcaaaatc cagtcgaccg
    19261 tcgcggattg ccggtaagaa cgaagacagt tgcgcttcat acatagaaac ctgcgctttc
    19321 gggaacacct ctttgaactt gttgatcatc cctgacataa aagtaaaacc aatcaatgaa
    19381 ggaaaaccaa atgagacttc caccaccgct tcagaagaca taccgcttat ctcattaacc
    19441 atatttttca tttcacgggt aatggattcg gaacgggaga gtaacaattg accggcaggt
    19501 gttaatgtta caccggtatt tttccgcacc actaattcca caccaaaata atcttcaata
    19561 tcgttaatga ttttactgac ggccggttga gttaacccta attcttttgc agccgagccg
    19621 atagagccac ttctaatgac ttcctgaaag actaccaggt gctgcgtttt cggaagaaga
    19681 atagtgctca taacgaccta cgttaattac ctcattgacg gcatgaagtg tatcaaaatg
    19741 aaataaacag gatatgtgcg accactcaca aattaacttt cattactttc caaggtatcg
    19801 ttattacaat taaacatctt taaatatcaa caagttaaag cataaaaatc agcataaacc
    19861 ctgattttta tcaaaaaaaa atgacgggga taaccatatt ttatggcgat aacatcattc
    19921 gttatgtggt taatacaaaa aaggggctga gaccatattt caagcaatta ccggataatt
    19981 accaggcaaa atttgagtta aatttaatga gcaagttctc aaattttatt ataaataaac
    20041 cattttcagc gataaatact gcggcacgtc acattttttc acgctatttg ttggagaaca
    20101 aacatttatt ttatcaatat tttaaaattt cgaatacagg tattgatcat ctcgaacaat
    20161 tgattaacgt caactttttc tcttctgaca ggacgtcatt ttgtgaatgc aatcgttttc
    20221 cataaattct tctcccctca taggcgacga atagcatttt gtgttgagga tcacaaaacg
    20281 aataattgct ggtcgccgcg ataaggtcag acgaagtcaa caagggggat tttcactgtg
    20341 ctttacatca gcgtaggaca cagaatgttg cttcgtttac tgtaacgccg ggtaaatgag
    20401 cgttttttga tagtgcgtaa gtgtcgtgcg aggaaaataa gcatctatta ttgtttgtca
    20461 tgacgacaag gcaggcatca atgaaaggct tcccaattgc acatattttt cacccttcaa
    20521 tcccgccaat gcacgcagtg gttaacaatc acaatagaaa tattgattat tggacggtaa
    20581 aaagaaagtt tgcagaaatt gtctccacca atgacgttaa taaaatttac agtataagta
    20641 atgaactccg gagagtatta tctgcaatta ctgcattgaa tttctatcaa ggcgatgttc
    20701 cttctgtcat gattccgaat ccaaccggaa aatatgagtc cattcatcat agatatttct
    20761 acaggagaac atgatgatta tatcatacaa acattagatg taggcacttt tgcacctttt
    20821 ggtgaacaat gtacttgctc agccgtcaat aaaaaagagc tggaatgtat taaggagacg
    20881 atttctaagt attgcgcaaa gttcacccaa aaagaagcca tcttaacacc ccctaggcac
    20941 tttaataaaa cctgcattac ttcagattgt tggcaaattc tctttttttc acccgatcat
    21001 tttaataatg atttttattg attatttata aaggataaaa taatgttccc tgtttcatca
    21061 attggtaatg atattagtag tgatttagtc cgtcgaaaaa tgaatgacct tcctgaaagc
    21121 cccataggaa ataacctcga agcactagca ccaggtatag aaaaactaaa acagacctct
    21181 attcaaatgg tcactttact taacgcgtta caaccaagtg gaaaatgcat tatcactggt
    21241 gattttcaaa aagaattagc gtacttacaa aatgtaattc tttataatga ctcgtctctt
    21301 cgtctggatt ttttaggtta taacacccaa attattcaac gatcggacaa tacctgcgaa
    21361 cttaccatta atgaaccgtt aaaaaactag gaaatatcta caggcaatat caatgttaat
    21421 tgcccattaa aagatattta caatgaaatc aggaggttga acgtaatctt tagttgtggg
    21481 actggagata tagttgatct atcctctctg gacttacgta atgtcgattt agattattat
    21541 gatttcacag ataaacatat ggctaatact attctaaatc attttaaatt ggattcaaca
    21601 aattttacta atgccaacat gtttcaggtt aattttgtta gttcaacaca aaacgccaca
    21661 atctcctggg attatttact aaaaataacg cctgttttaa taagcattag cgatatgtat
    21721 tctgaagaaa aaatcaagtt tgtcgaaagt tgtttaaatg agcttggaga cattaccgaa
    21781 gaacaattaa aaattatgag atttgcaatt ataaaatcta taccaagggc aactcttaca
    21841 gataaattag aaaatgaatt aacaaaagaa atatataaaa gctcatcgaa aatcaacaat
    21901 tgcttgaaca gaattaaatt accagagatg aaagaattct catcaaaaaa aacatatgat
    21961 tacatcgata caatcattga agattatgaa aatatcaaag aaaatgctta tctggtcgtc
    22021 ccccaaatta attatactat ggatttaaac atagaagact ctagctcaga agagttaata
    22081 ccctcgagaa agacgaaaat tctccggaca atggctttga ggtcggggaa tataacacat
    22141 atgaagcata taactcagag aatcaatatt ttaccagaga ggactatacg tataattacg
    22201 accttttaaa tgcaatatag acttaataac ttcttacaaa gtctattatt aataccgtag
    22261 atattcatct taccctgtgc accaaaagca aaaacttatc gacgcgggtt tgaaactgat
    22321 ggcaaccgca aaaatgcctg atgcgctacg cttatcaggc ctacgccatc tctgcaatat
    22381 attgaatttg cgtgcttttg taggcaggat aaggcgttca cgcagcatcc ggcatgaata
    22441 aagcgcactc aacaatctga aacccgccgg aacggtttat tacgtacatc aggtgaaact
    22501 gaccgataag ccgctttctt ttgggtatag tgtcgtggac agtcattcat ctttctgccc
    22561 ctccaaaagc aaaaacccgc cgaagcgggt ttttacgtat atcaggtgaa
//
LOCUS       DAJTUI010000062        22278 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTUI010000062 DAJTUI010000000
VERSION     DAJTUI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.062"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..1025)
                     /locus_tag="OO464_002588"
     CDS             complement(<1..1025)
                     /locus_tag="OO464_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6227690.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1127..2107)
                     /locus_tag="OO464_002589"
     CDS             complement(1127..2107)
                     /locus_tag="OO464_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR6227691.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2115..2825)
                     /locus_tag="OO464_002590"
     CDS             complement(2115..2825)
                     /locus_tag="OO464_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR6227692.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3042..4865)
                     /gene="typA"
                     /locus_tag="OO464_002591"
     CDS             complement(3042..4865)
                     /gene="typA"
                     /locus_tag="OO464_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6227693.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5238..6647
                     /gene="glnA"
                     /locus_tag="OO464_002592"
     CDS             5238..6647
                     /gene="glnA"
                     /locus_tag="OO464_002592"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6227694.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6820..7869
                     /gene="glnL"
                     /locus_tag="OO464_002593"
     CDS             6820..7869
                     /gene="glnL"
                     /locus_tag="OO464_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6227695.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7881..9290
                     /gene="glnG"
                     /locus_tag="OO464_002594"
     CDS             7881..9290
                     /gene="glnG"
                     /locus_tag="OO464_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6227696.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9402..9512
                     /locus_tag="OO464_002595"
     CDS             9402..9512
                     /locus_tag="OO464_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6227697.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="OO464_002596"
     CDS             complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="OO464_002596"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6227698.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="OO464_002597"
     CDS             complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="OO464_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6227699.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12394..13026
                     /locus_tag="OO464_002598"
     CDS             12394..13026
                     /locus_tag="OO464_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6227700.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13408..16194)
                     /gene="polA"
                     /locus_tag="OO464_002599"
     CDS             complement(13408..16194)
                     /gene="polA"
                     /locus_tag="OO464_002599"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6227701.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16558..17490
                     /locus_tag="OO464_002600"
     CDS             16558..17490
                     /locus_tag="OO464_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6227702.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17531..18961)
                     /locus_tag="OO464_002601"
     CDS             complement(17531..18961)
                     /locus_tag="OO464_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6227703.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19116..19742)
                     /gene="dsbA"
                     /locus_tag="OO464_002602"
     CDS             complement(19116..19742)
                     /gene="dsbA"
                     /locus_tag="OO464_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6227704.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19759..20745)
                     /gene="srkA"
                     /locus_tag="OO464_002603"
     CDS             complement(19759..20745)
                     /gene="srkA"
                     /locus_tag="OO464_002603"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6227705.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20822..21091)
                     /locus_tag="OO464_002604"
     CDS             complement(20822..21091)
                     /locus_tag="OO464_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6227706.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21161..21745
                     /gene="mobA"
                     /locus_tag="OO464_002605"
     CDS             21161..21745
                     /gene="mobA"
                     /locus_tag="OO464_002605"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6227707.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21727..22254
                     /gene="mobB"
                     /locus_tag="OO464_002606"
     CDS             21727..22254
                     /gene="mobB"
                     /locus_tag="OO464_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6227708.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5515 a         5583 c         5333 g         5847 t
ORIGIN      
        1 gcttcgccga taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc
       61 agcgtacatg ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt
      121 acgagcgcag cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg
      181 atcttatctg aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta
      241 atgatgccgt aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga
      301 atgaagaatg tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc
      361 cagatggttt tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct
      421 tttttcacct caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg
      481 cctacggcaa tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca
      541 ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca
      601 cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt
      661 accgacttga gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag
      721 acaaagagga tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc
      781 gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac
      841 agaaaaaagc ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca
      901 ttggtcaaat ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat
      961 ttataaattg taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg
     1021 agcataacat cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac
     1081 cattcatatg gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa
     1141 gatggtttgc ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa
     1201 ttgcatcttc tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg
     1261 tttcactcat ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg
     1321 ccgacgcata gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat
     1381 cgccaaggca gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa
     1441 tacaggcttt atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga
     1501 cctgggtaat atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga
     1561 tcatgttagc aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg
     1621 acaccagctc atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt
     1681 cctgatacgt ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca
     1741 aattaccttc ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg
     1801 cgacggcttc gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt
     1861 tcagacggcg ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag
     1921 aaaaacgctc gccatccaga acatggattt tgacgccacg tagattattt tcactggcga
     1981 tatcaagtaa atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag
     2041 catgtaaata aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg
     2101 tcaccataaa acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca
     2161 atatctatct ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct
     2221 ggcatacccg cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg
     2281 aaaatcaatt tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat
     2341 gagtggtttt caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg
     2401 tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt
     2461 gatggcgtca ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc
     2521 tcggtaaacc cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc
     2581 gacagctccg gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta
     2641 cggaagattt tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta
     2701 atgcccagca gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg
     2761 ccatctttca accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc
     2821 gccattgttt aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa
     2881 gaaagcaatt aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta
     2941 atcacagact tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa
     3001 cctgccatcg ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg
     3061 cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc
     3121 ggagtcactt ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg
     3181 cggataggcg gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt
     3241 ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc
     3301 tgaccttcgt aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg
     3361 aacagcgcga acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc
     3421 tgacccactt cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca
     3481 gtaccggaag tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc
     3541 acgtagtcga gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta
     3601 cgttcgccca gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg
     3661 ttttcatacg gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat
     3721 accgccagtt cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca
     3781 ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg
     3841 ttgtgtacca gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct
     3901 tctttaccgc agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca
     3961 acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc
     4021 agttcgccca ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata
     4081 cgttccagac cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg
     4141 ctatcgatga tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg
     4201 ccgataacgc caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg
     4261 tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg
     4321 tcttccgcca tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg
     4381 atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga
     4441 tccacaaccc aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc
     4501 ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca
     4561 aatgcgtcaa ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca
     4621 ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata
     4681 gcggtgtttt tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc
     4741 acgcgctctt gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct
     4801 accagggtgg ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg
     4861 atcacaactt tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact
     4921 acaaaacagg atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc
     4981 tttcacggag cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt
     5041 tccattgaag cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg
     5101 ttaagcatga taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct
     5161 tttttacggc gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa
     5221 tccaggagag ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag
     5281 tgaagtttgt tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc
     5341 ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga
     5401 ttggcggctg gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg
     5461 cagtgattga cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac
     5521 ctggcaccct gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt
     5581 acctgcgttc cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc
     5641 tgttcgatga catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata
     5701 tcgaaggcgc atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg
     5761 cagtgaaagg cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg
     5821 aaatgtgtct ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag
     5881 cgactgctgg tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg
     5941 aaattcagat ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga
     6001 cctttatgcc aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc
     6061 tgtctaaaaa cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg
     6121 cgctgtacta cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc
     6181 cgaccaccaa ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt
     6241 actctgcgcg taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac
     6301 gtcgtatcga agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc
     6361 tgctgatggc cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca
     6421 aaaacctgta tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc
     6481 tggaagaagc actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt
     6541 tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc
     6601 gtatgactcc gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt
     6661 actacccggc gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca
     6721 tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata
     6781 atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc
     6841 gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg
     6901 gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg
     6961 tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa
     7021 gaaagtctgg aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt
     7081 cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag
     7141 atggcaccga tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa
     7201 gtagctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt
     7261 ttacgtggcg cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat
     7321 accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg
     7381 ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta
     7441 acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc
     7501 ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc
     7561 aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg
     7621 tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat
     7681 aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc
     7741 cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca
     7801 ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc
     7861 aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt
     7921 ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg
     7981 agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag
     8041 atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc
     8101 caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct
     8161 atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc
     8221 tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta
     8281 acggcccaac gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta
     8341 tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta
     8401 aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg
     8461 cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga
     8521 aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg
     8581 gcacgttatt tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc
     8641 gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg
     8701 tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc
     8761 gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac
     8821 gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg
     8881 gcgtagaagc gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc
     8941 caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc
     9001 aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta
     9061 cttcgcaaat gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc
     9121 tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac
     9181 tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct
     9241 ggggccgcaa cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt
     9301 gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat
     9361 aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta
     9421 atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc
     9481 tgtgtgccgc gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga
     9541 aagtcgtagg cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg
     9601 atgcggcgta aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc
     9661 cagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta
     9721 aacgaagcgc cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg
     9781 cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc
     9841 acctgtattc ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc
     9901 ttgagatctt ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg
     9961 taatccagac ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca
    10021 cgcgttagcg caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac
    10081 tgcttcaact ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg
    10141 cccatcccca gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc
    10201 acgccttcac gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg
    10261 ataaactgat agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt
    10321 tgctgcggac tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc
    10381 agatgcgcgt agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg
    10441 gtaaaggcga aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg
    10501 gaggtaaaac caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc
    10561 tcgcggttaa ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga
    10621 ttaaagcctt cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact
    10681 tcgatcgaaa tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga
    10741 cggctgattt gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg
    10801 acgtgacgcc cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga
    10861 tactgatcgg ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa
    10921 agcttatggc agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc
    10981 acggcttgta aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac
    11041 gaggtgtatc gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt
    11101 tgctgtacag acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg
    11161 gatgcccgtt tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct
    11221 gtagtttaac gaataaccac accagataac atataaccgg aagggttatc agcagaacgg
    11281 gaaggcccac ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct
    11341 tctcgtcttc ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact
    11401 catcaatacg atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc
    11461 ctgcttccag acgttccagc agcgcatcca gacactcatc cgtttccagt aattccaact
    11521 ccgcctgcgg tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt
    11581 cagtcactcc caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct
    11641 ggcctttgct gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac
    11701 gacgtttttt ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc
    11761 gacgcgcttt tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc
    11821 gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa
    11881 gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc
    11941 ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt
    12001 tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct
    12061 tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc
    12121 tatgggtgct actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca
    12181 cattttcaga gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat
    12241 cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg
    12301 gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc
    12361 gccacaaagc tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg
    12421 cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg
    12481 gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag
    12541 aaaagcctgg ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa
    12601 gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa
    12661 gagatgaagc gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg
    12721 caaggtctgg tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg
    12781 attgagtggg cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa
    12841 ctggcaagcg gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt
    12901 aacggtgatg tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg
    12961 aggcagaaac tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc
    13021 gaataatttt cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa
    13081 tagattacaa ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt
    13141 ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc
    13201 aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac
    13261 gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag
    13321 ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta
    13381 cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc
    13441 cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg
    13501 cttcgcgacg gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg
    13561 catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc
    13621 ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc
    13681 cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc
    13741 cagcgtttca acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag
    13801 cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat
    13861 gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc
    13921 tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc
    13981 cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg
    14041 cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata
    14101 atcctctggc gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat
    14161 gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata
    14221 agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt
    14281 cgacttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag
    14341 cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag
    14401 cggtttaata ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt
    14461 aaattcttca cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag
    14521 ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg
    14581 tgaaagcacc ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg
    14641 tttctgcaga tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc
    14701 ggcgtaacgt ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt
    14761 acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc
    14821 catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc
    14881 aatcccacgc agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg
    14941 cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc
    15001 gtgagagatt tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac
    15061 gcctggctcg atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc
    15121 ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag
    15181 tgtttcttca tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc
    15241 tggcgcttcg tctgcaacac tggtttcctg aggcttcgcg gctgtttttg cccctttggc
    15301 ctgtaaccat ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa
    15361 cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc
    15421 cagttcaaca tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt
    15481 ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc
    15541 tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt
    15601 ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa
    15661 gtcgatgatc aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat
    15721 ggtattcgtc atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt
    15781 atcgccagtg ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc
    15841 gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac
    15901 catcgcgtgc agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga
    15961 tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc
    16021 tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat
    16081 cgcaccggtc ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata
    16141 aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc
    16201 cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt
    16261 ttcaccgctt ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt
    16321 ggataacttt gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag
    16381 catgatgttt gtctttgttt ttcatgcagt taaaaataaa tccatcccta ataattgaga
    16441 taatgggatt ttttctaatg tggatagata tgaattattt ttctccttaa ggatcatccg
    16501 ttatttgggt cattttctgc taaaatctgc gccttgcgct tctttagtgc actgtctatg
    16561 gctaaccttt tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg
    16621 agtatcgtat tgaccatttt ggtcactatt ttttgttccg tcccgatcat cattgccggg
    16681 attgtaaaac tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat
    16741 tttatgatgt attgctggtg tgaaggtctg gcggtattac tgcatctgaa cccacacctg
    16801 caatgggaag ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt
    16861 aaccaccgta gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg
    16921 atgaataaat atttcctcaa gcaacagctg gcctgggtgc catttcttgg cctggcgtgc
    16981 tgggcgctgg atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg
    17041 gagcgacgtg gtcaagatgt tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat
    17101 cccaccacca ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa
    17161 acccactcca cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta
    17221 aatgtactgg gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat
    17281 aaccgtcagc cgttcttcga tatgttaagc ggtaaactga cgcggattgt cgtacatgtg
    17341 gatttacagc ctattgccga tgagttacat ggcgactaca tcaatgacaa aagctttaag
    17401 cgccattttc agcaatggct gaactcattg tggcaggaaa aagaccggct tctgacgtct
    17461 ctcatgtcat cacagcgtca gaataagtaa gttttactcg cctctgaatg agcagaggcg
    17521 agtgagtatt ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct
    17581 cctgtccgtt aaaaacaact ttacccggtg tataacgcag ctgcagcgag gcggtattgt
    17641 tgtcaatcgt gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat
    17701 cagcctgctt ttgcgctttt tccgcatcca ttccttcaga taaattaagc tgttttgcgg
    17761 tttctgtgac gacattaagc ggtaacttca catcaaaatt gagcgatttg atatctttgt
    17821 ttgtggatga tgaagatttg gctgggtcgg caatactgat atccagattg gcatttagtt
    17881 cgccgagtgc gttcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata
    17941 acggtaaata ctctttgaac aatgcagcat tcacttcttg cagagcaact tcatcgtttg
    18001 ctaactcagg gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgataaatt
    18061 ggcgcactgc tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt
    18121 gctgattccc ttttgttaac ttatcaagtt cgtaggtcag attgacattg atataatctt
    18181 tgtctttaac gcgatccagc ctggtttttg caacgaaagc atcgatttgg gcaacgtctt
    18241 ccccgtgatt gataatgtta atctgattaa atttcgactc gctttcacca accggaaatt
    18301 tcttttcttc taatcgcgcc agagaatcaa tggtaaaact ttttgccgtt actttgttca
    18361 ttggtgataa ttgcagaaca atatcttcga ctttgccttc aatatccagg ttagacaatg
    18421 aactaccatc accattaaaa gtaaactggc cctcaccaaa agaaaatttt ccatactctg
    18481 caggggcaac atctaatgtc gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt
    18541 ccacgggcga tttatttttc gcggcgataa atagcggttg tgtcagttca ttattcacta
    18601 agttcacttt tgccgctgcc agtgctggga tgacattacc atgcattaac attgtgatgg
    18661 gcaacggacc gtgctcaaca tccacatcaa atacaacttt ctggcctggc ttgatattga
    18721 gatcgggtgc accgttatcg aatgtcattt gcatttgaaa acgggaatta aaaaagcctt
    18781 tgtcaaagtt tttataactt aacgtcatat cgtaggcatg ttcacctttc tttttcgcat
    18841 cgttaaaatc tttaataaac ttttcgatac caggctgaat ttgcgtacct gtgtaccatg
    18901 tgccaccacc ccagattacg gctaacgcaa caataacacc tgtagctgac ttacgtatca
    18961 tataaatatt ccctgataaa acagcaaaac tttgaatatc cacttatgct gaaataatgt
    19021 ccagcggcag gatgcattat cagaatgtat ttatatttat taaataataa aaaaagcccg
    19081 tgaatattca cgggctttat gtaatttaca ttgaattatt tttcctcgga cagatatttc
    19141 accgtatcag catactgctg aacaaaaaca tccatattgc tggtatccat accctgcgga
    19201 ttcagctgat atttaccgtt aacaaacatc gccggaacac cacgcaattg cacatcagct
    19261 gcggcttttt cctgctgagc gaccagagat ttcaccacga agctgttcca cgccgcgtcg
    19321 tactcttcac ctttaatacc tgcattgata aatacatcgc ggatatcaga tgctgaacga
    19381 atggtctggg ttttctgtac gccttcaaac agcggaacag tcactttgtc ttccacgccc
    19441 agcgccatcg ccacagccca tgcctgagtc agatctttgc ccaggtcacc acccatgaag
    19501 ttgacgtggt atttagtcat cttcacgcct tccggcagtt ttttcttcac attatcagaa
    19561 atatgcagaa cttcttcaaa ctgatagcag tgcgggcaga agaaagagaa aaactccagc
    19621 acttgcggcg cgccagctac cggtttttcc agggtagtgt actgtttacc atcttcatac
    19681 tgcgccgccg atgcgctaaa cgctaaaact aaaccagcca gcgccagcca aatctttttc
    19741 atgatctact ctctccgatt aatacatagg tgttaattgc aaagggggtt cttgtagaac
    19801 ttttgcctgt tctataaaag tcgccgtctg tcgtagccag taatcttccc cggttaacca
    19861 cgggaaattt ttcgggaacg cgggatcagc ccaatgccgc attagccagg caagataata
    19921 aaccaaacgc atggcgcgta aaggttcaat cagtccgatt tcagcggtgt cgaactcgct
    19981 aaattcttca taagcttcaa taatagtttc cagttgcatc cgctgctggg ctttatcgcc
    20041 attgagcaac atccacaaat cctgaatagc tggaccatta cgtgcatcat ccagatcaac
    20101 aaacattgga ccatcgcgcc agagaatatt cccggcgtgg cagtctccat gtagccgcag
    20161 aacggtgaaa tcttcccgcc agtgtgctgt aacggcggcg atcagctcat ctgtcgcttt
    20221 cagaaatgcc gctttcaacc cggaaggtat cagtgtagcg tcctcaaaca gcttgcgagg
    20281 ctcaatgaga tattcattca gaccgatggt cgggcgatga ataaaaagct gtttgcgccc
    20341 cgtctggtgc atacgcccta aataacgccc aaccgcttcc atctgatcga tattatcagc
    20401 ttcgaactgg cgaccaccga cgcttggaaa aacagcgaaa taaaatccct gatgattcaa
    20461 taaagtctga ccgttaaagg ccacaggtgc tgcgaccgga acttcatcat ttaccagctg
    20521 caacgcaaat tgatgttctt cgaggatttg atcggctgtc caacgttcag ggcgataaaa
    20581 tttgacgaca aaacgtcgac gctcttcgtc ctgaaattga tagacacggt tttcatagct
    20641 gttaagcggg gtaagaccgg aatccacccg gatcccttgc ttaaacagag cgtccatgat
    20701 ggtatccggg tgtagtgtct ggaaagtaaa agcgctgttg ttcatcccat catccggaaa
    20761 acattacgaa tgatgaagga tatcatttca cgatgatttt ggtggcgccg cttacaagct
    20821 tttactcttt aattacgccg cgagcgcgta acagcgccgt tttgaagtct tcttcataat
    20881 ctttctgcaa acccgggatc accgcatctt tcgcggaatc acgcattttc aggtgataga
    20941 tcagaatgtc atccgtcaaa tccgccagtt cgccgtcaaa acctgactct ttcgccagtt
    21001 tctgcaaaaa ttgcaacaag ttaaagtctg gctctttttg ccaggctggc tggaggagtt
    21061 caataacttc attcagacgt ttacatttca tagtaatgct ccttactctt gagacagaca
    21121 cgttagcagg gtcaatccca caataaaaga ggcgatatcg gtgaatctaa tgacgacgat
    21181 aacaggcgtt gtgctggcag gcggtaaagc cagacgaatg ggcggcgtag ataaaggatt
    21241 gctggactta aatggcaaac cgctgtggca acatgtcgct gacgcgctta agacgcagct
    21301 ctctcacgtc gtggttaatg ctaatcgtca tcaggaaatc tatcaggcag gcggtctgaa
    21361 agtgattgaa gattcactgg cggattaccc aggccctctg gcaggaatgc tttcagtaat
    21421 gcagcaggaa gctggtgagt ggtttttgtt ttgtccgtgc gatacgcctt acattcccca
    21481 tgatttagcc gcccggctta atcatcagcg caaagatgcg cctgtcgtgt gggtccatga
    21541 cggtgaacgc gatcacccga ctattgctct ggtaaaccgc gctattgagc ctttattact
    21601 ggaatatctg caagcaggag aacgccgggt aatggcattt atgcgtctgg ctggcggtca
    21661 tgcggttgat ttcagcgatc ataaagatgc atttgttaac gtgaatacgc cagaggagct
    21721 tgcccgatgg caggaaaaac gatgataccg ttactcgcct ttgccgcgtg gagtggtacc
    21781 ggaaaaacta cgcttttgaa aaaactgatc ccggcattat gcgccagagg gatccgtccg
    21841 gggctgatta agcatacgca ccatgatatg gatgttgata agccaggcaa agatagctat
    21901 gagctgcgca aggctggcgc ggtacaaacc atcgttgcca gccagcaacg atgggccttg
    21961 atgacggaaa caccagacga agaagagctg gatctacatt ttctcgcaag tcgaatggat
    22021 acctcaaagc tggatttgat tctggtcgaa gggtttaagc atgaagagat cgcaaagatt
    22081 gtgctgtttc gcgatggagc cggacatcga cctgaagaat tagtgataga caggcatgtt
    22141 attgctgtag ccagtgatgt gccgcttaat cttgatgtcg cattactgga tattaatgat
    22201 gttgaggggc tggctgattt tgtggtggag tggatgcaaa agcagaacgg ataagcaaga
    22261 gtaggctgga tcagacgc
//
LOCUS       DAJTUI010000063        21514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTUI010000063 DAJTUI010000000
VERSION     DAJTUI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.063"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO464_002607"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO464_002607"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6227709.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="OO464_002608"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="OO464_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6227710.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="OO464_002609"
     CDS             complement(2649..3200)
                     /locus_tag="OO464_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6227711.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="OO464_002610"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="OO464_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6227712.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="OO464_002611"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="OO464_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="OO464_002612"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="OO464_002612"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6227713.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="OO464_002613"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="OO464_002613"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6227714.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="OO464_002614"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="OO464_002614"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6227715.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="OO464_002615"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="OO464_002615"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR6227716.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="OO464_002616"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="OO464_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6227717.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="OO464_002617"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="OO464_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6227718.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="OO464_002618"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="OO464_002618"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6227719.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="OO464_002619"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="OO464_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6227720.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="OO464_002620"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="OO464_002620"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6227721.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="OO464_002621"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="OO464_002621"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6227722.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="OO464_002622"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="OO464_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6227723.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18170..18565)
                     /locus_tag="OO464_002623"
     CDS             complement(18170..18565)
                     /locus_tag="OO464_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6227724.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="OO464_002624"
     CDS             complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="OO464_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6227725.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19481..20074
                     /locus_tag="OO464_002625"
     CDS             19481..20074
                     /locus_tag="OO464_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6227726.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20219..20671
                     /gene="tabA"
                     /locus_tag="OO464_002626"
     CDS             20219..20671
                     /gene="tabA"
                     /locus_tag="OO464_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6227727.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20794..>21514
                     /locus_tag="OO464_002627"
     CDS             20794..>21514
                     /locus_tag="OO464_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6227728.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKALN"
BASE COUNT         5197 a         5324 c         5668 g         5325 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccagt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tatgcatttt tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca
    18061 gacatttcct gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt
    18121 catccgactt atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag
    18181 ggatacgcgc tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg
    18241 ccgtccagtt tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt
    18301 cagattgttt ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc
    18361 atcccgcagc tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa
    18421 aatcgggttc tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat
    18481 cgccggaacg aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag
    18541 ggaaaacagc ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag
    18601 atttatattt atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat
    18661 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    18721 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    18781 agcgacctct tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg
    18841 cattggcccg ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat
    18901 ggtgatccct cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
    18961 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    19021 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
    19081 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    19141 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    19201 aacgacatca ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt
    19261 ctcttgtgcc agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    19321 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    19381 gagaactgtc ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    19441 attacaaaac tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc
    19501 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    19561 gctttttcac caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg
    19621 tattaacccc ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    19681 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
    19741 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    19801 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
    19861 tgatattgcc attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
    19921 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    19981 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc
    20041 aggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    20101 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg
    20161 atcatctttt cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat
    20221 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    20281 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg
    20341 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    20401 ggagtaccat gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac
    20461 cttcagcacg caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc
    20521 atttttgccg gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt
    20581 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
    20641 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    20701 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    20761 cttagacgtt cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat
    20821 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg
    20881 tatgtgggaa tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt
    20941 ctttctccta gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat
    21001 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct
    21061 tattctaaag atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg
    21121 gagcgagcgg atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa
    21181 gggattcgtg aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt
    21241 tatgaaggga agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag
    21301 gttctggcgt gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat
    21361 cagaagttaa ataatataca ggagttaaat gattcgcagg agttaaataa ttcacagaag
    21421 ttaaataatt cgcaggagtt aaataactcg caggtgagtt gtaaaggttc agttgattct
    21481 acgattacgg atttattaga aaaagcattg aata
//
LOCUS       DAJTUI010000064        21499 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTUI010000064 DAJTUI010000000
VERSION     DAJTUI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.064"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(42..2111)
                     /gene="glyS"
                     /locus_tag="OO464_002628"
     CDS             complement(42..2111)
                     /gene="glyS"
                     /locus_tag="OO464_002628"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6227729.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2121..3032)
                     /gene="glyQ"
                     /locus_tag="OO464_002629"
     CDS             complement(2121..3032)
                     /gene="glyQ"
                     /locus_tag="OO464_002629"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6227730.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3127..3426)
                     /locus_tag="OO464_002630"
     CDS             complement(3127..3426)
                     /locus_tag="OO464_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6227731.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3601..4596
                     /gene="wecH"
                     /locus_tag="OO464_002631"
     CDS             3601..4596
                     /gene="wecH"
                     /locus_tag="OO464_002631"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6227732.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4638..5075)
                     /locus_tag="OO464_002632"
     CDS             complement(4638..5075)
                     /locus_tag="OO464_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR6227733.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5121..5462)
                     /locus_tag="OO464_002633"
     CDS             complement(5121..5462)
                     /locus_tag="OO464_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227734.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5631..7085)
                     /gene="xylB"
                     /locus_tag="OO464_002634"
     CDS             complement(5631..7085)
                     /gene="xylB"
                     /locus_tag="OO464_002634"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR6227735.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELVRAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7157..8479)
                     /gene="xylA"
                     /locus_tag="OO464_002635"
                     /pseudo
     CDS             complement(7157..8479)
                     /gene="xylA"
                     /locus_tag="OO464_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8845..9837
                     /gene="xylF"
                     /locus_tag="OO464_002636"
     CDS             8845..9837
                     /gene="xylF"
                     /locus_tag="OO464_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6227736.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9915..11456
                     /gene="xylG"
                     /locus_tag="OO464_002637"
     CDS             9915..11456
                     /gene="xylG"
                     /locus_tag="OO464_002637"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR6227737.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11434..12615
                     /gene="xylH"
                     /locus_tag="OO464_002638"
     CDS             11434..12615
                     /gene="xylH"
                     /locus_tag="OO464_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR6227738.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12693..13223
                     /locus_tag="OO464_002639"
     CDS             12693..13223
                     /locus_tag="OO464_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6227739.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13335..13871
                     /locus_tag="OO464_002640"
     CDS             13335..13871
                     /locus_tag="OO464_002640"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6227740.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13979..14803)
                     /locus_tag="OO464_002641"
     CDS             complement(13979..14803)
                     /locus_tag="OO464_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR6227741.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15123..17153
                     /gene="malS"
                     /locus_tag="OO464_002642"
     CDS             15123..17153
                     /gene="malS"
                     /locus_tag="OO464_002642"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6227742.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17331..18584
                     /gene="avtA"
                     /locus_tag="OO464_002643"
     CDS             17331..18584
                     /gene="avtA"
                     /locus_tag="OO464_002643"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR6227743.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18735..19208)
                     /locus_tag="OO464_002644"
     CDS             complement(18735..19208)
                     /locus_tag="OO464_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6227744.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19310..20158)
                     /gene="yiaJ"
                     /locus_tag="OO464_002645"
     CDS             complement(19310..20158)
                     /gene="yiaJ"
                     /locus_tag="OO464_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR6227745.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20358..21356
                     /gene="yiaK"
                     /locus_tag="OO464_002646"
     CDS             20358..21356
                     /gene="yiaK"
                     /locus_tag="OO464_002646"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR6227746.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21368..>21499
                     /locus_tag="OO464_002647"
     CDS             21368..>21499
                     /locus_tag="OO464_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6227747.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     "
BASE COUNT         5406 a         5488 c         5275 g         5330 t
ORIGIN      
        1 aaaaagcctg ccagatggca ggctttttaa taacggcgtt attactgcaa cagcgaaata
       61 tccgcaacgc gcaggaacag ttcgcgcagt ttctccagca tggtcagacg gttgagacgc
      121 aattctttgt catcaaccat gaccatcact ttatcgaaga aagcatcaac cggctcacgc
      181 agctctgcca gttcgaccag cgcatcctgg taacgacctt ccgcaaagta cggctccagc
      241 ttgtcacgta gcaccacaac ctgcatcgcc agtttaattt cttccggctc tttcagggta
      301 gaggcattca cgcgatcgct cagcacttcg tcagatttcg ccaggatgtt agatacacgt
      361 ttgttcgccg ccgccagtgc tgcagctgct tccagggtac ggaagtgcga taccgctttc
      421 atgcgggcat cgaaatcagc cggacgagtc ggacgacgcg ccagtaccgc ctggatggtg
      481 tcaacggtat aaccttcgtc ctgataccag gcgcggaagc gaccgagcat aaagtcgata
      541 acatcatcaa ctacgttggc attagtcagc ttatcgccat acagacgcac cgcttcttcg
      601 gtcagcgttt gcagatcaag gttgaggttc ttctcaacga taattcgcag cacgccaagt
      661 gcggcacgac gcagcgcaaa cgggtctttg tcgcctttcg gatgctgacc gataccgaag
      721 atacccgcca gagtatccat cttatcagca atcgccagcg cacaagctac cgggttggac
      781 ggcaggtcat caccggcaaa gcgcggctga tactgctcat tcagcgcgac ggctacgtct
      841 tccgcttcgc catcgtgacg cgcgtagtgc atccccataa cgccttgggt gtcggtgaac
      901 tcgaagacca tgttggtcat caggtcacac ttagacagca ggcccgcacg ggttgcgtgg
      961 ttaacgtcag cgccaatctg ttcggcgatc cagccagcca gcgcctggat gcggtcagtc
     1021 ttgtcacgca gcgtacccag ctgttgctgg aacagcacgg tttgcaggcg cggcaggtta
     1081 tcttccagac gttttttgcg gtcggtgttg aagaagaact cggcatccgc cagacgtgga
     1141 cgaacgactt tctcgttacc agagataatt tgctgcggat ctttcgattc gatgttggca
     1201 acgaagataa agttcggcag cagtttgccg tcgttcgcat acaccgggaa gtacttctgg
     1261 tcacctttca tggtgtaaac cagggcttca gccggcaccg cgaggaattt ctcttcgaat
     1321 tttgcggtca gaacgaccgg ccactccacc agcgaagcca cttcttccag caggctttcg
     1381 cttaagtcag cgttaccgcc aatcttacgc gctgcttctt cggcatcggc tttaatcttc
     1441 gctttacgtt cttcgtaatc ggcgatgact ttcccgcgct cacgcagaat ttccggatac
     1501 tgatcggcgt tgtcgatggt gaattccggt tcgcccataa agcggtggcc gcgaatcacg
     1561 cgatcggact gaatgcccag aatggttgcc ggaatgactt tgtcgcccag cagcagggtc
     1621 acggtgtgca ccggacgcac gaagtgcacg tcgcttgcac cccagcgcat cagtttcggg
     1681 atcggcagtt tcgccagcga agtcgcaacc atattcggca gcagtgcttc ggtgctttcg
     1741 cccttcacat gggcgcgata cagcagccat tcgcctttat cggtagtcag acgctcagcc
     1801 tggtcaacgg taataccaca accacgcgcc caaccttctg ccgctttgct cggttttcct
     1861 tcagcgtcga acgcctgggc aatcgccggg ccgcgttttt cgatttcacg atctggttgt
     1921 gcttccgcca ggttagctac tttcagcgcc agacgacgcg gagcagcaaa ccattgaacg
     1981 gtgccgtgtg cgaggcctgc gttatccagc tccgcagtaa agttcgcagc aaaggactct
     2041 gccaggctgc gcagtgcttt tggtggcagc tcttcagtgc cgatttccac cagaaaagtt
     2101 ttctcagaca tagccgcctc ttacttatct ttgttgcaca tcgggaagcc gagggcttca
     2161 cgggaagcgt aatatgcttc tgccactgct ttggtcaggg tgcgaatgcg cagaatgtag
     2221 cgctgacgct cggtgacgga gatggcttta cgcgcatcca gcaggttgaa gctgtgggcg
     2281 gctttcagaa tacgctcgta ggctggcagc ggcagcggat tttccagcgc cagcagctgc
     2341 tgcgcttctt tctcgtactg ctcaaagcag gtgaacagga agtccacatc cgcgtattcg
     2401 aagttgtaag tggactgctc cacttcgttc tgatggaaca cgtcgccgta ggtggtttta
     2461 cccagcgggc cgtcgctcca gactaggtcg taaacgctgt ctacgccctg aatgtacatg
     2521 gccagacgtt ccagaccgta ggtgatctcg ccggtaaccg gtttacactc cagaccacca
     2581 acctgctgga agtaagtgaa ctgcgtcact tccatgccgt ttagccacac ttcccagccc
     2641 agtccccagg cccccagagt cgggttttcc cagttatctt ccacgaaacg gatgtcgtga
     2701 atagtcgggt ccatgcccag ctctttcaga gaaccgaggt acagctcctg aatattgtcc
     2761 ggcgatggct taatgaccac ctggaactga tagtagtgct gtaaacggtt ggggttttcg
     2821 ccgtagcgac catcggtcgg gcgacgagaa ggctgaacat aagcagccgc catcggttct
     2881 ggccccagcg cgcgcagaca ggtcattggg tgagaggttc ccgcgccgac ttccatgtcc
     2941 aatggttgaa caatggtgca gccctggcga gcccagtaat cctgtaaggt caggatcaag
     3001 ccctggaagg tcctggtatc aaacttttgc atattatttc gtgctggata cgtgtggatt
     3061 taaaggaagg gatcagtata cccgctggat ggaagatata cagtacgaaa cgggaaaaag
     3121 cagggcttaa cgcatggaaa gatgcaaaaa atggccatct gcgtcaaaag aacagacaaa
     3181 ctgctcttta cggaaggtat aaccgcgcat ttcatagctt ccctggaact gttcgaaggc
     3241 ggtgacgtcg attttttgct cgccggtgtt ataacgtttt gccgcctgat ccttgcataa
     3301 cgcttccata ttcaacgaac gcagaggatc aacccttacc cgttgtgctt tctgcacggg
     3361 tgacggtgta gaacaaccca ctagcaccat aagcgccatt gccggaaaga aagcgttcat
     3421 catcattaaa ttaccgtctg gtttgcaggt gagtcttatt atttatatcg gtatagtggc
     3481 tgatatctta cgaatttcct taagcctcta taggctgtag tgataaaact cagattattc
     3541 cagtgcgcca accaaatgat ttatcggtca gacttttttt aaccagatac agagacacag
     3601 atgcagccca aaatttactg gattgataac ctgcgaggga tagcgtgttt aatggtggtg
     3661 atgattcaca ccactacctg gtatgtgacc aatgctcata gtgttagccc cgtcacatgg
     3721 gatatcgcca atgttctgaa ttctgcctct cgtgtcagcg tgccgctatt tttcatgatt
     3781 tccggctatc tcttttttgg cgaacgcagc gcccagccgc gccatttctt gcgtatcggc
     3841 ttatgtctga ttttttatag cgcaatcgca ctgctctaca ttgcgctgtt tacctccatc
     3901 aatatggagt tagcgctgaa aaacctgctg caaaagccag tgttttacca cttgtggttt
     3961 ttcttcgcga ttgcggtgat ttatctggtt tcaccgctga ttcaggtgaa gaacgtcggc
     4021 ggaaaaatgt tgctggtgct aatggtggtg attggcatta tcgctaaccc aaacacagtg
     4081 ccgcagaaaa ttgacggttt tgaatggctg ccaattaact tatatatcaa tggcgatact
     4141 ttttactaca ttctgtatgg catgttgggc cgcgctatag ggatgatgga cacacagcat
     4201 aaagcactgt cgtgggtgag cgccgcgctg tttgcgacgg gggtttttat tatctctcgc
     4261 gggacattat atgaattgca gtggcgcgga aattttgccg atacctggta tctttactgt
     4321 gggccgatgg tttttatctg cgcaatcgcg ctattgactc tggttaaaaa cacgctggat
     4381 acgcgtacca ttcgcggact tggcttaatc tcccgccatt cattgggtat atacggattc
     4441 cacgccttga ttatccatgc gctgcgcacc cggggaattg agcttaaaaa ttggccaata
     4501 ctggatatta tttggatctt ttgcgcgacg ttggcagcga gtttgttact ttctatgctg
     4561 gtacaacgaa tcgacagaaa cagattagtg agttaagtaa aagcccgttc acattggact
     4621 gaccgggctt acgtgaatta ttcgctgtat tcttcctggg taacctgtgt ttcttttggc
     4681 gggtttattc cggcatcacg aatattcttc tgcaccgcta ctgcaccaaa caagcttaag
     4741 aagaaagcca gtccataaaa acctttttca ctgagtaata atgtcgcatt ccacagacct
     4801 accatcagta gagcaacaga gataataaag acagtcaggc aggtcatata ataaatggaa
     4861 gtggtcggta tgccttcata cttgtcccga acggtctttt gataagacgc agcagaaaac
     4921 agtcctaaca ccagtacggc aaaataatac cctttttcat ttaactgcat ctctgcattc
     4981 catagcccta acaggtaggt aacgatacca ccaacgagag ctatccatga cacaatacta
     5041 aaggccggtg aataggttga tattttgttg tccatcacag tattcctttc attcctgaat
     5101 gtgtaagagc tttatgttgc tcataccgag cgaaacaaac gcatttgacc aaacaagcag
     5161 acaaaaaagg cgatggggta gatcaccatt tcataagcat tgataggtgc atttaaaaca
     5221 ccgataaaca agagaccgat agtgattaac gcaactaact ggcagatatg ggtaaaaaaa
     5281 ttatccagtt gcccgagttt ttctgcgcga agatatgcat agttaccaaa agttgctgtc
     5341 attaacacgc caagaaaata gccttttcca cttagcaatg ggcatgagat ccatagccca
     5401 accagataaa ccagcgcccc gacaacaaat aatagttttg ccacagtttt tgaggttttc
     5461 ataacgatct ccatatctac cagcgataca ttacgagtaa ccaacgaaag acaaaactga
     5521 aaaatgccat taacaaatga ttttcagaat aaattcatac taaatattaa ttaattagtg
     5581 agatatatag atgtgaatta tcccccaccc ggtcaggcag gggataacgt ttacgccatt
     5641 aatggcagaa gttgctgata gaggcgacgg aacgtttcac gtcgtggctg ataagcggca
     5701 taacgctgcg catctggtag atgcgactgt tctaacggta gctgcggcaa caactcaatg
     5761 agcgatttct ctggattcgc cgcaatctgc gccagccttg ctgcgcccag tgctggcccc
     5821 acatcccccc ccgtacggta atcgagctgc tgaccgctga tatccgccag catctgacgc
     5881 cagtactcac tacgcgcccc gccaccaatc aacgtaacac tttgcggttt aatgccgcag
     5941 gcatgcacga catccatgcc atctgccagc gcataaccca cgccttccag cactgctcgc
     6001 accagttcat tggggccatg ttgatgagtc aaaccaaaga aaaccccctt cgcctgggga
     6061 ttattgtgtg gtgtacgctc gccggaaaga taaggcagaa accaaactgg ctcggcactt
     6121 tcatcagcct gttgagctgc agcgattaaa gctgggacat tgcacaggcc ggttaatttc
     6181 gcggcccaat ccagacacga tgctgcactc agcattacag acattaaatg ccaacgctgc
     6241 ggtagcgcat ggcaaaagct gtgtacagcg ctttctggct tgcttaagaa cccttcgctg
     6301 acagcaaaat agacccccga cgtccccagc gataacattg cctgattagc atcaaccatt
     6361 cccacaccaa ctgcaccagc tgcattgtcg ccaccgcctg cgacaactgg caccgtcgcc
     6421 ataccccacg ctttcgcaac ttcaggtaac aaagcaccag taatttcgct gccttcgtat
     6481 aatgcgggca tctggtcacg agataagtcg caagcctgca gcatgacgtc actccagtca
     6541 cgctttgcga catccagcca catggtgcca gctgcgtcag acatatcgct ggcaaactcc
     6601 cccgtcatac gcagacgcaa gtaatctttc ggtaataata ctttgtcgat ttgacggaat
     6661 atctccggct catgccgctg aacccatagc aatttaggcg cagtaaatcc gggcatcatc
     6721 aggttgccgg taatcactcg tgattgcgga actctcgctt ccagcaaagt gcactcttgc
     6781 gcacagcgcc cgtcgttcca caaaatggca gggcgcaata cccgttgttg agcatccagt
     6841 aaggttgctc catgcatctg gccggcaata cccaatgctt taacgtcctg cagagaatgc
     6901 tgatcgccca gagctttcat tgcgcgatca gttgcctgcc accactgttc cgggtcttgt
     6961 tccgaccaaa gtggatgcgg gcgcgaaacg gtcagctttt ccgtttgcga agcaaccacc
     7021 tcaccctgct cgttgagcaa aataactttt acgcccgagg tgccaagatc tatcccgata
     7081 tacatatcga tcgttcctta aaaaaatgcc cggtatcgct accgataacc gggccaacgg
     7141 actgcacagt tagccgttat ttgtcgaaca gataatgatt taccagattt tccagttgct
     7201 cctggcgacc actctgatgc accggagaca aattatgttc ctgagcatat ttggctaaat
     7261 ctgccagtga catttggcct ttcaggattt gctggcccaa ttcgctattc cagccggaat
     7321 aacgctgcgc gatgcgttta tccagctcgc catcttcaat catgcgcgct gcaattttca
     7381 gcgccagtgc catcgtatcc atcgcgccga tatgaccgta aaacagatca tatttatcag
     7441 tactttgacg acgtactttg gcatcgaagt tcagaccacc ggtggtgaaa ccgcctgctt
     7501 tgagaatttc atacatcacc agcgcattct cttccacact gttcgggaac tggtcggtgt
     7561 cccagcccag ttgcgcatcg ccacggttgg cgtcgacaga accgaacagg ccaagcgcaa
     7621 tggcggtggc tatttcatga tggaaagagt gacctgccag cgtcgcgtgg ttagcttcaa
     7681 tgttcagttt aatctctttt tccagaccaa actgtttcag gaagccatag accgtcgcgg
     7741 catcgtaatc atattgatgt ttagtcggtt cttgcggttt cggttcgata agcaacgtgc
     7801 cctagaagcc gattttatgt ttatgctcaa ccaccatctg cataaagcgg cccagttgtt
     7861 cacgctcctg acgcaagtcg gtatttaaca gcgtttcgta accttcacga ccaccccaca
     7921 ggacatagtt ttcaccgccc aatttatggg ttgcttccat cgctgtaaca acttgcgttg
     7981 ccgcccagct gaagacttca ggatctgggt tcgtcgccgc acccgcgcca tagcgagggt
     8041 ttgtaaagca gttagcggtt ccccacagca gcttcacgcc gctctcttct tgcttggctg
     8101 ccaggacatc aaccatttgc gcaaaattat tgatgtactc ttttaacgac gcgccctcag
     8161 gggaaacatc cacatcgtgg aagcaataaa atggcacatg taacttgtgg aaaaactcaa
     8221 atgcgacatc tgctttacgc ttcgccaacg ccagtgcctc accaggctgc tgccacggac
     8281 gattaaacgc ccccacacca aacatgtccg ccccgttcca gcagaaggtg tgccagtagc
     8341 aggcggcaaa acgcaagtgc tcttccatac gcttacccaa caccagttcg tcgggattgt
     8401 agtgacggaa tgctaacggg tttgaggatt ttgagccttc ataacgaacg cgatcgagct
     8461 ggtcaaaata ggcttgcata ttgaactcca taatcaggta atgccgcggg tgatggatga
     8521 tgtcgtaata ttgggcactc cctttcaatt gctcaattat gttatttcac actgctattg
     8581 agataattca caagtgtgcg ctcgctcgca aaataaaatg ggatgatgaa aatgggtaat
     8641 tcctcgaaga taaaaatgca ataagtacaa ttgcgcaaca aaagtaagat ctcggtcata
     8701 aatcaagaaa taaaccaaaa atcgtaatcg aaagataaaa atctgtaatt gttttcccct
     8761 gtttagttgc taaaaattgg ttacgtttat cgcggtgatt gttacttatt aaagctgtcc
     8821 tctaactaca gaaggcccta caccatgaaa ataaagaaca ttctactcac cctttgcacc
     8881 tcacttctgc ttaccaacgt tgcggcacac gccaaagaag tcaaaatagg tatggcgatt
     8941 gatgatctcc gtcttgaacg ctggcaaaaa gatcgagata tttttgtgaa aaaggcagaa
     9001 tctctcggtg cgaaagtatt tgtacagtct gcaaatggca atgaagaaac acaaatgtcg
     9061 cagattgaaa acatgattaa ccggggtgtc gatgttcttg tcattattcc gtataacggt
     9121 caggtattaa gtaacgttgt aaaaaaagcc aaacaagaag gtattaaagt attagcttac
     9181 gaccgtatga ttaacgatgc ggatatcgat ttttatattt ctttcgataa cgaaaaagtc
     9241 ggtgaactgc aggcaaaagc cttggtcgat attgttccgc aaggtaatta cttcctgatg
     9301 ggcggctcgc cggtagataa caacgccaag ctgttccgcg ccggacaaat gaaagtgtta
     9361 aaaccttacg ttgattctgg aaaaattaaa gtcgttggtg accaatgggt tgatggctgg
     9421 ttaccggaaa acgcattgaa aattatggaa aacgcgctaa ccgccaataa taacaaaatt
     9481 gatgctgtag ttgcctcaaa cgatgccacc gcaggtgggg caattcaggc attaagcgcg
     9541 caaggtttat cagggaaagt agcaatttcc ggccaggatg cggatctcgc aggtattaaa
     9601 cgtattgctg ccggtacgca aactatgacg gtgtataaac ctattacatt gctggcaaat
     9661 actgccgcag aaattgccgt tgaattgggc aatggtcagg agccaaaagc agatacctca
     9721 ctgaataatg gcctgaaaga tgtcccctcc cgcctcctga caccgatcga tgtgaataaa
     9781 aacaacatca aagatacggt aattaaagac ggattccaca aagagagcga gctgtaagcg
     9841 ttacgccccg gcgcggagcg ggggcgtgat ttctctccat gccgcgtgaa tgaattggct
     9901 taggtggagt cgttatgcct tatctacttg aaatgaagaa cattaccaaa accttcggca
     9961 gtgtgaaggc gattgataac gtcagcttgc ggttgaatgc tggcgaaatc gtctcactct
    10021 gtggggaaaa tgggtctggt aaatcaacgc tgatgaaagt gctgtgtggt atttatcccc
    10081 atggctccta cgaaggcgaa attatttttg cgggagaaga gattcaggcg agtcacatcc
    10141 gcgataccga acgcaaaggt atcgccatca ttcaccagga attggccctg gtgaaagaat
    10201 tgaccgtgct ggaaaatatc ttcctgggta acgaaataac ccacaatggc attatggatt
    10261 atgacctgat gacgctacgc tatcagaagc tgctcgcaca ggtcagttta tccatttcac
    10321 ctgatacccg cgttggcgat ttagggcttg ggcaacaaca actggttgaa attgccaagg
    10381 cacttaataa acaggtgcgc ttgttaattc tcgatgaacc gacagcctca ttaactgagc
    10441 aggaaacgtc ggttttactg gatattattc gcgatctaca acagcacggt atcgcctgta
    10501 tttatatttc gcacaaactc aacgaagtca aagcgatttc cgatacgatt tgcgttattc
    10561 gcgacggaca gcacattggt acgcgtgatg ctgccggaat gagtgaagac gatattatca
    10621 ccatgatggt cgagcgagag ttaaccgcga tttaccctaa tgaaccacat accaccggag
    10681 atgaaatatt acgtattgaa catctgacgg catggcatcc ggtcaatcgt catattaaac
    10741 gagttaatga tgtctcgttt tccctgaaac gtggcgaaat actgggtatt gccggactcg
    10801 ttggtgccgg acgtaccgag accattcagt gcctgtttgg cgtgtggccc ggacaatggg
    10861 aaggaaaaat ttatattgat ggcaaacagg tagatattcg taactgtcag caagccatca
    10921 cccaggggat tgcgatggta cccgaagaca gaaagcgcga cggcatcgtt ccggtaatgg
    10981 cggttggtaa aaatattacc ctcgccgcac tcaataaatt taccggtggc attagccagc
    11041 ttgatgacgc ggcagagcaa aaatgtattc tggaatcaat tcagcaactc aaagttaaaa
    11101 cgtcgtcccc cgaccttgct attggacgtt tgagcggcgg caatcagcaa aaagcgatcc
    11161 tcgcccgctg tctgttactt aacccgcgca tcctcattct tgatgaacct accaggggta
    11221 ttgatattgg cgcgaaatat gagatctaca aattaattaa ccaactcgtc cagcagggta
    11281 ttgccgttat tgtcatctct tccgaattac ctgaagtgct cggccttagc gatcgtgtac
    11341 tggtgatgca tgaagggaaa ctaaaagcca acctgataaa tcataacctg actcaggagc
    11401 aggtgatgga agccgcattg aggagcgaac atcatgtcga aaagcaatcc gtctgaagtg
    11461 aaattggccg taccgacatc cggtggcttt tctgggctga aatcactgaa tttgcaggtc
    11521 ttcgtgatga ttgcagctat catcgcaatc atgctgttct ttacctggac caccgatggt
    11581 gcctacttaa gcgcccgtaa cgtctccaac ctgttacgcc agaccgcgat taccggcatc
    11641 ctcgcggtag gaatggtgtt cgtcataatt tctgctgaaa tcgacctttc cgtcggctca
    11701 atgatggggc tattaggtgg cgtcgcggcg atttgtgacg tctggttagg ctggcctttg
    11761 ccacttacca tcattgtgac gttggttctg ggactgcttc tcggtgcctg gaacggatgg
    11821 tgggtcgcgt accgcaaagt cccttcattt attgtcaccc tcgcgggcat gttggcattt
    11881 cgcggcatac tcattggcat caccaacggc acgactgtat cccccaccag cgccgcgatg
    11941 tcacaaattg ggcaaagcta tctccccgcc agtaccggct tcatcattgg cgcgcttggc
    12001 ttaatggctt ttgttggttg gcaatggcgc ggaagaatgc gccgtcaggc tttgggttta
    12061 cagtctccgg cctctaccgc agtagtcggt cgccaagctt taaccgctat catcgtatta
    12121 ggcgcaatct ggctgttgaa tgattaccgt ggcgttccca ctcctgttct gctgctgacg
    12181 ttgctgttac tcggcggaat gtttatggca acgcggacgg catttggacg acgcatttat
    12241 gccatcggcg gcaatctgga agcagcacgt ctctccggga ttaacgttga acgcaccaaa
    12301 cttgccgtgt tcgcgattaa cggattaatg gtagccatcg ccggattaat ccttagttct
    12361 cgacttggcg ctggttcacc ttctgcggga aatatcgccg aactggacgc aattgcagca
    12421 tgcgtgattg gcggcaccag cctggctggc ggtgtgggaa gcgttgccgg agcagtaatg
    12481 ggggcattta tcatggcttc actggataac ggcatgagta tgatggatgt accgaccttc
    12541 tggcagtata tcgttaaagg tgcgattctg ttgctggcag tatggatgga ctccgcaacc
    12601 aaacgccgtt cttgattttg ataaaaattt tctcaaagcc ggttacgtat taccggtttt
    12661 gagtttttgc atgattcagc aggaaaagaa ccatgtttac taaacgtcac cgcatcacat
    12721 tactgttcaa tgccaataaa gcctatgacc ggcaggtagt agaaggcgta ggggaatatt
    12781 tacaggcatc acaatcagaa tgggatattt tcattgaaga agatttccgc gcccgcattg
    12841 ataaaatcaa ggactggtta ggagatgtcg tcattgccga cttcgacgac aaacagatcg
    12901 agcaagcgct ggctgatgtc gacgtcccca ttgttggggt tggcggctcg tatcaccttg
    12961 cagaaagtta cccacccgtt cattacattg ccaccgataa ctatgcgctg gttgaaagcg
    13021 catttttgca tttaaaagag aaaggcgtta accgctttgc tttttatggt cttccggaat
    13081 caagcggcaa acgttgggcc actgaacgcg aatatgcatt tcgtcagctt gtcgccgaag
    13141 aaaagtatcg cggagtggtt tatcaggggt tagaaaccgc gccagagaac tggcaacacg
    13201 cgcaaaatcg gctggcagac tgactacaaa cgctgccacc gcaaaccggg attattgccg
    13261 ttactgacgc ccgagcgcgg catattctgc aagtatgtga acatctacat attcccgtac
    13321 cggaaaaatt atgcgtgatt ggcatcgata acgaagaact gacccgctat ctgtcgcgtg
    13381 tcgccctttc ttcggtcgct cagggcgcgc ggcaaatggg ctatcaggcg gcaaaactgt
    13441 tgcatcgatt attagataaa gaagaaatgc cgctacagcg aattttggtc ccaccagttc
    13501 gcgtcattga acggcgctca acagattatc gctcgctgac cgatcccgcc gttattcagg
    13561 ccatgcatta cattcgtaat cacgcctgta aagggattaa agtggatcag gtactggatg
    13621 cggtcgggat ctcgcgctcc aatcttgaga agcgttttaa agaagaggtg ggtgaaacca
    13681 tccatgcaat gattcatgcc gagaagctgg agaaagcgcg cagtctgctg atttcaacca
    13741 ccttgtcgat caatgagata tcgcaaatgt gcggttatcc atcgctgcaa tatttctact
    13801 ctgtttttaa aaaagcatat gacacaacgc caaaagagta tcgcgatgta aatagcgagg
    13861 tcatgttgta gcgatgatga gaattgtcgg cgtcacatca gggaactcag cacagccacc
    13921 tgatgcgagc aaaagcctta tccgaccgac aaatcagata aggctcgagg aaatgaaatt
    13981 acatatgcgc cgcgattaac cgttggttat cctggtacat tgcgaacagg tagttgttat
    14041 aactcttccc tttggtcgag tagcccttca gcttgtgaat catcgctgtg gcagtcactt
    14101 cctgatccgc tttacgcagc tgtgcacgcg atttacggaa cgaagagtaa gccgggtgcg
    14161 tattcaggtt agtgacatag gcgctcaccg attctttgac agaactaaac tgtgagtacc
    14221 ctttcacttt acccggcgca ttggtacaac gtcctttcat gcatttcatg ccgaacagat
    14281 tgttattgtt gcgcgccagc ttcgacgttc cccaaccgct ttctgctgca gccatcgtcg
    14341 ccaccatact ggtagggata atgtctacgc gttcaagcaa ggtattccac gggatttttc
    14401 gcgtattacc agaccacttc accttgtagc gtttggcgat gtctttcaga cgcgcacgct
    14461 cagcaggtgc ccattggccc tgatactgtt ttgaaatcag ccagttacgt tctgcagtaa
    14521 tggctgcatt ttggctggta atgtaaggca ttacggtccg gagaaacgcc tttttccttg
    14581 gtgttccgga agggtatttt cgcaaatcag gaagtgaact actctttgca ctattgcgag
    14641 aatactcttg tttactgctt acctgtttat tactagcttt agttaagtgg gacttttgac
    14701 tcgctgttgt tgtgtgcgtc ttcgctaaca cctcactcga aaacaccaga gtgagtaaca
    14761 taagaatcat cgccccatat cgtcgtatgg gagtcaaaat catcaggtct cctggtcgga
    14821 tttaatcatt ccaacacctt atatttttca caaatttgag agttgaatct caaatcatat
    14881 caaaaatagc tgtcaagagc accccaagga atagtccaaa tctgaaacta tgtcacgagt
    14941 taacgattca gattggcgct aaatcgcaga aaatgtgggg gttatcgcaa aattcagccg
    15001 ttttttgcgc gcgatcgctc acccttgctt ctcatcctgt ggacttaccg ctcagggatg
    15061 agttttgtct ggcttatcgc tggcaaactg tctgaaatcg cagcaataag gactcatcca
    15121 ccatgaaact cgccgcctgt tttctgacac tccttcctgg cttcgccgtt gccgccagct
    15181 ggacttctcc ggggttccct gcctttagcg aacagggaac gggaacattt gtcagccacg
    15241 cgcagttgcc caaaggtacg cgtccactca cgctaaattt tgaccaacag tgctggcagc
    15301 ctgcagatgc gataaaactc aatcagatgc tttccctgca accttgtagc aacacgccgc
    15361 ctcaatggcg attgttcagg gacggcaaat atacgctgca aatagacacc cgctccggta
    15421 cgccaacatt gatgatttcc atccagaacg ccgccgaacc ggtagcaaac ctggtccgtg
    15481 aatgcccgaa atgggatgga ttaccgctca cgctggatgt cagcgccact ttcccggaag
    15541 gagccgccgt acgggattat tacagccaac aaattgcgat agtgaagaac ggtcaaataa
    15601 cgttacaacc cgctgctacc agcaacggtt tactcctgct ggaacgggca gaaactgaca
    15661 catccgcccc tttcgactgg cataacgcca cggtttactt tgtgctgaca gatcgtttcg
    15721 aaaacggcga tcccagtaat gaccagagtt acggacgtca taaagacggt atggcggaaa
    15781 ttggcacttt tcacggcggc gatttacgcg gcctgaccaa caaactggat tacctccagc
    15841 agttgggcgt taatgcttta tggataagcg ccccatttga gcaaattcac ggctgggtcg
    15901 gcggtggtac aaaaggcgat ttcccgcatt atgcctacca cggttattac acacaggact
    15961 ggacgaatct tgatgccaat atgggcaacg aagccgatct acggacgctg gttgatagcg
    16021 cacatcagcg cggtattcgt attctctttg atgtcgtgat gaaccacacc agctatgcca
    16081 cgctggcgga tatgcaggag tatcagtttg gcgcgttata tctttctggt gacgaagtga
    16141 aaaaaacgct gggtgaacgc tggagcgact ggaaacctgc cgccggacaa acctggcata
    16201 gctttaacga ttacattaat ttcagcgaca aaacaggctg ggataaatgg tggggaaaaa
    16261 actggatcag aacggatatc ggcgattacg acaatcctgg attcgatgat ctcactatgt
    16321 cgctggcctt tttgccggat atcaaaaccg aatcaactac cgcttctggt ctgccggtgt
    16381 tctataaaaa caaaacggat actcacgcta aagtcatcga aggctttaca cctcgcgatt
    16441 acttaaccca ctggttaagt cagtgggtcc gcgactatgg gattgatggt tttcgggtcg
    16501 ataccgccaa acatgttgag ttgcccgcct ggcagcaact gaaaaccgaa gccagctccg
    16561 cgcttcgcga atggaaaaaa actaaccccg acaaagcatt agatgacaaa cctttctgga
    16621 tgaccggtga agcctggggc cacggcgtga tgcaaagtga ctactatcgc cacggcttcg
    16681 atgcgatgat caatttcgat tatcaggagc aggcggcgaa agcagtcgat tgtctggcgc
    16741 agatggatac gacctggcag caaatggcgg agaaattgca gggtttcaac gtgttgagct
    16801 acctctcgtc gcatgatacc cgtctgttcc gtgaaggggg cgacaaagca gcagagttat
    16861 tactattagc gccaggcgcg gtacaaatct tttatggcga tgaatcctcg cgtccgttcg
    16921 gtcctacagg ttctgatccg ctgcaaggta cacgttcgga tatgaactgg caggatgtta
    16981 gcggtaaatc tgccgccaac gtcgcgcact ggcagaaaat cagccagttc cgcgcccgcc
    17041 atcccgcaat tggcgcgggc aaacaaacga cactttcgct gaagcagggc tacggctttg
    17101 ttcgtgagca tggcgacgat aaagtgctgg tcatctgggc tgggcaacag tgacttttcc
    17161 ggcttcccgt tcgtcagtac ctcgggaagc cgccaaccag gataaaatgt cagccctaat
    17221 cagcgttgcg ggataaagca ccgctcactc ttcaacagac cgatttgcac cccagcaaat
    17281 gtagcgttat tgttaccttc cttgctacag agttcgacag atatcccgct atgacattct
    17341 ccctttttgg tgacaaattt acccgccact ccggcattac gctgctgatg gaagatctga
    17401 acgacggttt acgcacgcct ggcgcgatta tgctcggcgg cggtaatccg gcgcagatcc
    17461 cggaaatgca ggactacttc cagacgctac tgaccgacat gctggaaagt ggcaaagcga
    17521 ctgatgcact gtgtaactac gacggtccac aggggaaaac ggagctactc acactgcttg
    17581 ccggaatgct gcgcgagaag ttgggttggg atatcgaacc acagaatatt gcactaacaa
    17641 acggcagcca gagcgcgttt ttctacttat ttaacctgtt tgccggacgc cgtgccgatg
    17701 gtcgggtcaa aaaagtgctg ttcccgctgg caccggaata cattggctac gctgacgccg
    17761 gactggaaga agatctgttt gtctctgcgc gtccgaatat tgaactgctg ccggaaggcc
    17821 agtttaaata ccacgtcgat tttgagcatc tgcatattgg cgaagaaacc gggatgattt
    17881 gcgtctcccg gccgacgaat ccaacaggta atgtgattac tgacgaagag ttgctgaagc
    17941 ttgacgcgct ggcgaatcaa cacggcattc cgctggtgat tgataacgct tatggcgtcc
    18001 cgttcccggg tatcatcttc agtgaagggc gcccgctatg gaatccgaat atcgtactgt
    18061 gcatgagtct ttccaagctg gggctgcctg gctcccgctg cggcattatc atcgccaatg
    18121 aaaaaatcat cactgccatc accaatatga acggcattat cagcctggca cctggcggta
    18181 ttggtccggc gatgatgtgt gaaatgatta agcgtaacga tctgctgcgc ctgtctgaaa
    18241 cagtcatcaa accgttttac taccagcgtg ttcaggaaac tatcgccatc attcgccgct
    18301 atttaccgga aaatcgctgc ctgattcata aaccggaagg agccattttc ctctggctat
    18361 ggtttaagga tttgcccatt acgaccgagc agctctatca gcgcctgaaa gcacgcggcg
    18421 tgctgatggt gccggggcac aacttcttcc cgggactgga taaaccgtgg ccgcatacgc
    18481 atcaatgtat gcgcatgaac tacgtaccag agccggagaa aattgaggcg ggggtgaaga
    18541 ttctggcgga agagatagaa agagcctggg ctgaaagtca ctaaaaattg cctgatgcgc
    18601 tacgcttatc aggcctacat gatttctgca atatattgaa tttggaagaa tttgtaggcc
    18661 ggataaggcg tttacgccgc atctggcata aacaaagcgc actattccgg cattgagaaa
    18721 cgccggaaaa cgtttcaaac aggctgctgc tgtagccgat gccgctgcac tttctcgaca
    18781 tccatgcact gcaacgcctt tgtcgggcag gcttcaacac aggccggccc cgtctcccga
    18841 tgccagcaca gatcgcactt aatcgccctc gcctttttgc gcgaagaaac gacctccatc
    18901 gcaccaaacg ggcaagccag catacagctt ttacagccaa tgcaacgtgt ttgttcaacg
    18961 aaaatatgcc catgttcgcg gcttatcgcg tcaacagggc agacattcgc gcacggtgca
    19021 tcttcacact gatgacaggc tactggcgtg gtccagctgt ggtctttaat gacacgaata
    19081 cgggaaataa actcgtctgg tgacaacgca gcgcaatcct gattctcctg atgcgacact
    19141 gcgcaagcca cttcacaggt acggcaaccg atacatttcg tcgcatccgc aataataaac
    19201 cggttcatga tcattccttt gcactaaaac agcagagtgc cagagagcgc gtccctactg
    19261 ctttgatcca acgaaggaaa tgttaaagtt ttggcacttg tccaaagcgt tatgtgatcg
    19321 cgcccaggtc atccctgaca gtaaatccca gttcattaga aatagcctgc gcggtttcac
    19381 gcagtggttt caggagattt ttctctccca cctgtttcag acgtgatgtc gaaagcgaaa
    19441 tcgacacggc gtacggcacc cgcccatgaa tatcaaacac cggaacagca atacaggaga
    19501 cgccgagttc gttttcttct ctgtccatcg ccgctccgct ttcacgaatg tgcgccagtt
    19561 cgtcgaacat cgcgggcagc tcggtaatgg tattgcgggt taacggctgg atctcatgct
    19621 gatggttttc ccagtatgac ttcacgtagt ccgggtgacc aaacgccatg tagatcttgc
    19681 ccattgcgga acagtagagc ggcatatgct ggccaatata ggcacgggtt cgcagcatcc
    19741 cggttgtggg ttccagctta taaatcaaaa tagcgtgatc gtcttcgcgg ctggagaagt
    19801 taatggtttc accagtggcg atgttcagtg cctcaagatg cggagcggcg atatgaatga
    19861 tattcagcga agacagcgcc ttctgcccga cggcaataaa tttggtagtc aggcggtaac
    19921 tccctgcggc gggcgcggtg gtcacatagc cgcaggactg taatccctgc aataagcgat
    19981 ggacggtact cttatttaaa ccagccagct ccgaaagatg cgccaacgga caaccgtttg
    20041 gatagttgct caaaatctca atcagcatca acccgcgaaa cagactctgg cttccggctg
    20101 gacgctcttt ttcctgcgcc atctcgttct ctttttttcc catcacttct ttccccattt
    20161 tgtcgcgtcc tgatggtagc gcaaagtgtg ccgtagttca cgatctcgac agataattta
    20221 taaccacttg atttttatgt cttttgaaat tcatcaatca gattgctttg ttaaaaagtg
    20281 atcgatatat ttgaaatcta gttccgcata ttgaaatttt aagccaaaaa agcgatcaaa
    20341 aaacaaggaa gcctaggatg aaagtgacat ttgagcagtt aaaagcagcc tttaatcggg
    20401 tcttaatttc acgcggcgtt gacagcgaaa cggctgacgc ctgtgcagag atgttcgccc
    20461 gcaccaccga atccggcgtt tattctcacg gcgttaatcg tttccctcgt ttcattcaac
    20521 aactggaaaa cggcgatatc attcctgatg cccaacccaa acgtataacc agcctcggcg
    20581 caattgaaca gtgggatgcc cagcgttcga tcggcaacct gacggcgaaa aagatgatgg
    20641 atcgcgccat tgaactggct gccgatcacg gtattggtct ggtggcactt cgtaatgcca
    20701 accactggat gcgcggcggc agctacggct ggcaggcggc ggaaaaaggc tatattggca
    20761 tttgctggac caactccatc gccgtaatgc cgccgtgggg cgcaaaagag tgtcgcatcg
    20821 gcactaaccc gctgatcgtc gccattcctt ccacgccgat caccatggtc gatatgtcga
    20881 tgtcgatgtt ctcttacggc atgttagaag ttaaccgcct ggcaggccgt cagctcccgg
    20941 tcgatggtgg ctttgatgat gagggcaatt tgaccaaaga acctggcgtt atcgagaaga
    21001 atcgccgcat tttgccgatg ggctactgga aaggttctgg catgtcgatt gtgctggata
    21061 tgatcgctac tctcctttcc gacggcgcat cggttgccga agtcacccag gacaacagcg
    21121 acgaatacgg catttcacaa atttttattg ccattgaagt ggacaagctt atcgacggtc
    21181 ccacccgcga tgccaagctg caacgtatca tggattacgt tactaccgct gaacgcgctg
    21241 acgaaaacca ggccatccgc ttacccggcc acgaatttac taccctgctg gccgaaaacc
    21301 gccgtaacgg catcactgtt gatgacagcg tgtgggccaa aatccaggcg ttatgaggag
    21361 ataagtcatg atttttggac atatcgcgca gcccaatccg tgccgtttgc ccgccgccat
    21421 tgaaaaggcg ctcgattttc tgcgcgctac caacttcaac gtcctggagc cgggcgttgt
    21481 cgaaatcgac ggcaagaat
//
LOCUS       DAJTUI010000065        21277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTUI010000065 DAJTUI010000000
VERSION     DAJTUI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.065"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..446)
                     /locus_tag="OO464_002648"
     CDS             complement(<1..446)
                     /locus_tag="OO464_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR6227748.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(501..1352)
                     /gene="manZ"
                     /locus_tag="OO464_002649"
     CDS             complement(501..1352)
                     /gene="manZ"
                     /locus_tag="OO464_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6227749.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1365..2165)
                     /gene="manY"
                     /locus_tag="OO464_002650"
     CDS             complement(1365..2165)
                     /gene="manY"
                     /locus_tag="OO464_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6227750.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2228..3199)
                     /gene="manX"
                     /locus_tag="OO464_002651"
     CDS             complement(2228..3199)
                     /gene="manX"
                     /locus_tag="OO464_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6227751.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3662..5212
                     /gene="yoaE"
                     /locus_tag="OO464_002652"
     CDS             3662..5212
                     /gene="yoaE"
                     /locus_tag="OO464_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6227752.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5216..6814)
                     /locus_tag="OO464_002653"
     CDS             complement(5216..6814)
                     /locus_tag="OO464_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6227753.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="OO464_002654"
     CDS             complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="OO464_002654"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6227754.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8493..9071)
                     /locus_tag="OO464_002655"
     CDS             complement(8493..9071)
                     /locus_tag="OO464_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6227755.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="OO464_002656"
     CDS             complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="OO464_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6227756.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10510..10689
                     /locus_tag="OO464_002657"
     CDS             10510..10689
                     /locus_tag="OO464_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6227757.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10809..11168)
                     /locus_tag="OO464_002658"
     CDS             complement(10809..11168)
                     /locus_tag="OO464_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR6227758.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11530..11874)
                     /locus_tag="OO464_002659"
     CDS             complement(11530..11874)
                     /locus_tag="OO464_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6227759.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12006..13916
                     /locus_tag="OO464_002660"
     CDS             12006..13916
                     /locus_tag="OO464_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6227760.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13974..14669
                     /gene="tsaB"
                     /locus_tag="OO464_002661"
     CDS             13974..14669
                     /gene="tsaB"
                     /locus_tag="OO464_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6227761.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14709..15290
                     /gene="yeaY"
                     /locus_tag="OO464_002662"
     CDS             14709..15290
                     /gene="yeaY"
                     /locus_tag="OO464_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6227762.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15496..17181
                     /gene="fadD"
                     /locus_tag="OO464_002663"
     CDS             15496..17181
                     /gene="fadD"
                     /locus_tag="OO464_002663"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6227763.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17263..18378
                     /gene="rnd"
                     /locus_tag="OO464_002664"
     CDS             17263..18378
                     /gene="rnd"
                     /locus_tag="OO464_002664"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6227764.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18432..19397)
                     /gene="yeaX"
                     /locus_tag="OO464_002665"
     CDS             complement(18432..19397)
                     /gene="yeaX"
                     /locus_tag="OO464_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6227765.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19453..20070)
                     /gene="yeaW"
                     /locus_tag="OO464_002666"
                     /pseudo
     CDS             complement(19453..20070)
                     /gene="yeaW"
                     /locus_tag="OO464_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20412..20978)
                     /locus_tag="OO464_002667"
     CDS             complement(20412..20978)
                     /locus_tag="OO464_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6227766.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21190..>21277
                     /locus_tag="OO464_002668"
     CDS             21190..>21277
                     /locus_tag="OO464_002668"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6227767.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5265 a         5274 c         5466 g         5272 t
ORIGIN      
        1 acgagaagct tataaatttt ttccagatcg tcgatatttc gaacgcggat taacagccga
       61 cgctgttcta attgcatcac caacacgcca tcttccgaca agttcatcgc tttgattcgg
      121 ctatattcaa tccagacatt ggcgaagaaa aaaccttttt gtttaaagat gatcttcgga
      181 atgcggatcc agaatatata aaaacccatc agagccagtg cacttaataa ccatgttgtt
      241 attagcgcgc catgattcgt gacgttgtta tagataagaa tgacaatcaa tccgacgaag
      301 ataacgctat cgatgcgacc acgccggagc aaaggaattg ccagcagggt ggggccgtta
      361 cggcggggca tgatgaactg atcgtagatc gcgaaggcca ggagtgcggc gatgaaaaga
      421 atcagtacca ggtccgtgat tgtcattaat cctccagata aaaaaacggg gccaaaaggc
      481 cccggtagtg tacgacagtc ttacagtccc agcaggccgc aagcgtaacc agcgataccg
      541 atgacgaaga agccaacgat gatccacagc gggttaactt ttttgcgcag cagccacata
      601 caagcaaatg tcagcagcag tggtacaagg cctggcatta actggtcaag aatagtttgc
      661 acggtggtga cacgggtctg tccatcctgc ccggtgatgg tcgaaaccac cagcgggatg
      721 ttgacatgtg tccacttgtt aaccaatgcc cccatgacaa acaggccgag gatagacgcc
      781 ccttccgtca gtttttgcag gaagccacca cccatatctt taacgatatc gatacctttg
      841 gagtaaccat acgctacgcc gtagtaacgg gttgccagac gcaccaggtt aaacaggatg
      901 aagaacagca gcggacctaa caggctgccg ctcatcgcga taccggcacc cagtgctgca
      961 aataccggac gtacggttcc ccagaagatc gggtcgccta caccagccag cggccccatc
     1021 aaaccgactt tgataccgtt gatagcaccg tcatcgatct ctgcgccatt agcacgctgt
     1081 tcttccagcg ccagggttac gccgagaatc ggtgcagcca cgaacggctg ggtgttaaag
     1141 aactccaggt gacggcgaat agcttgttta cgagcttcgt tgttctcagg gtagaggcga
     1201 cgaattgccg gtaccataga gaagcagaaa cccagtgcct gcatacgttc gaagttccat
     1261 gaaccctgga agaggttaga acgcaggaat acgccacgaa tatcactttg agtgagtttt
     1321 ttctcggtgg tagtttgagt tgtatcaacc atttcgctca cctgttagtc cagttcgtta
     1381 tcgagatcgt tgttaccagc tgcctgagca ggcgcaccgg ctacgcggtt gtatttcggg
     1441 ctaagttgga tgtagagcac tgccataaca gtaccaatca cacccagagc aaccaggtta
     1501 aagttggtga atgctgcggt tacgaagccg aggtagaaga acggcatcag gtagccagca
     1561 cgcatcatgt tgataaccat cgcataacca accaccacga tcatgccccc ggcgatattc
     1621 agaccattgg tcaccacttc cggaatcgca ttcagcatgt tctgtacttc gctggtacca
     1681 acagacagcg caacgatgac ggccggaata gccacacgca ttgcttgcag gaacagagaa
     1741 gaaacgtgga tccaggaaat cgctgtcagg ttgccgttat cagcagcctt atccgcagcg
     1801 tgctggaaag caacggtaat agtacgaacg atgatggtca gtacctggcc cgcagcggcc
     1861 agagggattg ccagtgcgat acctgcacca atgctctgat gacctgcgat aaccagaatg
     1921 gtagaaatga tagaagccag agcggcgtca ggcgcaactg cagcaccgat gttcatccag
     1981 cccagcgcga tcatttccag cgtaccaccg ataataatac cggttttcat atccccaaga
     2041 acgataccca ccagggtaca cgcgattagc ggacggtgaa actgaaattc atcgaggatt
     2101 gatcccatac ctgcgataca ggctacgata aataccagca caatttgaag agtggtaatc
     2161 tccattgtac ttctcctgtt tacgtaagac ttaagtgtga aaactgaatg ataatcaaca
     2221 caatacgtta cttatcgatt ttgctgatca gatccatcat tttcagtttc ggatcggtgg
     2281 aaaccttacg gacttccagc tcaataccgc gtgcattcag ttttttgaac gcctcgatat
     2341 ctttttcatc aaccgaaacc gcgttattca cctgggtttt accctgacgg aatgccatac
     2401 caccgacgtt aacagaggtg attttcacgc cgccttcaac gagacgctct acatctgttg
     2461 ggttggtaaa taacagcatt acgcgttcgc cagcatattt cgggttgttg tagacgcgaa
     2521 tcattttggc aacatcaact acgtgtgctg ttacgcccgg aggtgcaacc tgggtgagca
     2581 gtgtcttacg aacggtatcc gcagccactt catcactaac aacaataata cgggagacat
     2641 tggtttcttt ggtccagcgg gtggcgacct gaccgtgaat cagacggtcg tcgatacgcg
     2701 caaggccaat aaccatgtag tcgtttggcc ccattggttt tgccggagtt ggagccgctt
     2761 ttggtgctgc ggcagcgggt gctggcgcgg ctttttcaac cggtttggct ttcagtgctt
     2821 tcacgccttc acggcctgtt tctactgcca gcgcaaccag ttcatcaaag ctcgggtcat
     2881 catcacgggc cattaacgtt tccacgagca ttggaatgtt aacgcctgca atgacttcat
     2941 aatgctcttt gtcgacgaca atgcggctgg cagcattgaa cgggctgcct ccccatgtat
     3001 caacgagaaa cagcacgcct ttagtggtgt cgagttttgc caactgagcg ttgtattttt
     3061 caatcagcgt ttcggcattt tcacctggaa cgaaatcgat ccagccgacg ttttcctgct
     3121 cgcctaacag catttctgcc gttttaagca actgctctgc agcccaacca tgtgtgccta
     3181 taacaatagc aatggtcact tgctacctcc tttattatcg ttaacacctc aacgtgccag
     3241 atgtattttt gaatcgtcgt ccacaatcga atcgattcag ataagggcta caaccaaaaa
     3301 cccctgcgtt tcgcgattta ttttagatat cgaaaaaatt attttatgtg atgaagatcc
     3361 gtaatttaac tttcgattat cagaaatttc gaaaggtaaa atatccttgt cacattcgtt
     3421 tgcaaaggaa ggtaaatctt tgccaaatca gaggcgtctc tgatatgttt aactcccgtt
     3481 taacaaccat ggagtatagg gcagtagccc gcagtatgga tcgtcaccga cgtcatttca
     3541 gcatcaggcc ttttaacgcc tgcctttctg gcactctatg ccgcaccttt cgtttgcatt
     3601 ttgtcgttac gcctgcatta tttctggcgt cgaatagcta ttccttaagc aggagcttgt
     3661 catggaattc ttaatggacc cctcaatttg ggcggggcta ctcacgcttg ttgttctcga
     3721 aattgtgctg ggtatcgata acctggtctt catcgccatt cttgctgaca aactgccgcc
     3781 aaaacaacgc gataaagcgc gtttgctggg gttatcactg gcgctgatta tgcgtctggg
     3841 gctgctgtcg ctgatttcat ggatggtcac gctgaccaaa ccgctattta ccgtcatgga
     3901 tttctccttc tccggacgcg atctgattat gttgttcggg gggatattct tgctgttcaa
     3961 agcaacaacc gaactgcatg aacggctgga aaaccgcgat catgattccg gccacggtaa
     4021 aggctacgcc agtttctggg tggtcgtcac acagattgtc atccttgacg ccgtcttctc
     4081 gttggatgcg gtaattactg cagtagggat ggttaaccat ctgccggtga tgatggcggc
     4141 ggtagtgatt gcgatggcgg ttatgttgct ggcatcgaaa ccgctgacgc gattcgttaa
     4201 ccagcacccc acggtggtgg tgctctgtct gagcttcctg ttaatgattg gtctgagtct
     4261 ggtggcagaa ggtttcggtt tccacattcc gaaaggttac ctgtatgccg cgattggctt
     4321 ctcgatcatt atcgaagtgt ttaaccagat tgcgcgtcgc aactttattc gtcaccagtc
     4381 gactttgccg ctgcgagcgc gtactgccga tgccatcctg cgtttgatgg gcgggaaacg
     4441 tcaggccaat gttcagcacg atgccgataa cccgatgccg atcccggaag gtgcatttgc
     4501 cgaagaagaa cggtacatga ttaacggcgt actgacgctg gcgtcgcgtt ctctgcgcgg
     4561 gatcatgacg ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat
     4621 ccgcgagcaa ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga
     4681 tgaaatcatc ggtatcgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga
     4741 tgtggcggcg attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat
     4801 caacctgctg ggcgtgctgc gtcgcgctcg cgggagcttt gttatcgtga ccaacgagtt
     4861 tggtgtggta caaggcttgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt
     4921 cccggacgct gacgaaacgc cggaaatcat taccgatggt aacggctggc tggtaaaagg
     4981 cggtacagat ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga
     5041 tgatatcgcg acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg
     5101 cgatgtgatt gatgtagggc cactgcatat caccatcttt gaagccaatg attatcgtgt
     5161 tgatctggtt cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcgttaac
     5221 gtaacgacat aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg
     5281 ccagccattt gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa
     5341 tatgcacgcc atgacggcgc aaatacttcg cctgttcttg cgtttccacg ccctccgcca
     5401 ccagttcaat attcagtctt tgccccagcg cgatgatgat atcggtcacc gtcgagttaa
     5461 ccgcgtcaga acctatagct gcggtaaatg acttatcaat tttcagcacg tcaggacgta
     5521 atgtttcaag ccaggaaaac gagctgttgc cggtgccgaa gtcatcaatc gccagtttga
     5581 cgtttttacg atgcagctcg cgggcaatcc gataatcaac atccagtaag gcatcgcgtt
     5641 cggtgatttc gaggatcagt tgctgaatcg ggtgagcgct aaaccagtac tgattgagat
     5701 cttttatcag cacaccacgg cgaaaatggc tgggtgcgac gttaatgcca acatgaaact
     5761 gactactcat cgggaaaaca tggtgctgac gaatggtttc tgccatcaca tagtgggtca
     5821 gtggcacaat taaatgatgt tcttccgcga taggaataaa cacatccggt gaaatccagc
     5881 cctgacgcgg attattccag cgcagcaaaa tctctacacc aatacattgc tggctgcgcg
     5941 cattaagcaa aggctgacag aacaattcga actcatgttg cgccaggccc agattaattt
     6001 cgcgggaaaa gctcatccgg taagcggtcg ccagccaggc gatatagccc accagtaaac
     6061 ttagcagcac cgccagcggt aattgtgttg gaagatagtg ccatgccagc gccgtagccc
     6121 ccggaccatt tacgttaatg gtaaaaggaa agctttgcga agaaacctga tagttttcat
     6181 tgtcttcagg ttgcggaagg gaatctacca gcccattacc atagagcaaa tgccgtttgt
     6241 ccaccgtcag gctggcgcta ctgatttgcg gcagttgtgg ctcaaggagc attgccgtca
     6301 gtaagtcgat gttaatcatc tctatgaccc cagcattgct actgcccgct gcaggcgtcc
     6361 attgaatcaa aaccggactg cctttaatca gggcacggtc gatcgtcagg cgtaaaagtg
     6421 gttgcggtgc aggaagttca gccagaatgt ccacgacggg cacattgcga taaccaaaaa
     6481 tgctggagca ataaagtgtg ccgtcttgca ccaggccaat ggatcgcaca gtttggagtt
     6541 ttgccgcctg tttacgtaat ggcaaatggg cgacagagca gggcagacca atcagcggaa
     6601 gcaagacttt gctacctgcc tgtaggggaa gcagtacttt atctaattcc tctacagcgt
     6661 gattggcgaa ttgtactacc cgttgttgat ttaagttacg ctgtgaaata aatcgcacgc
     6721 tcagggtcga agcgagcgta acgagtgcgc aaatcgtaca aacaatcatt cgattacggc
     6781 gataggtttt aatgatccgt tgtgcttttt gcatgcgggc caccctgtaa gcgtatggcg
     6841 acaaacgcct gacgcaacag tggaagtgta gtggggaata ttacagcaga cgagaaagcg
     6901 ggtataaatt cgcccatccg ttgcagatgg gcgagtaaga agtattagtc acactggact
     6961 ttgattgcca gaccaccgcg tgaggtttcg cggtatttgg cgttcatgtc cttaccggtt
     7021 tcgtacatcg tttcgatgac cttatccagc gagacgcgcg gtgcactggt gcggcgcaga
     7081 gccatccgtg cggcgttaat cgccttcaca gaggcaatgg cattacgctc aatgcacggc
     7141 acctgaacct gccctgcaac cgggtcgcag gttaaaccaa ggttgtgttc catgccaatt
     7201 tccgccgcca cgcaaacctg ttccgggcta ccgcccagca gttctgcaag acccgcagca
     7261 gccattgaac aggcaacacc cacttcgccc tggcaaccaa cttccgcacc ggaaatagag
     7321 gcgttcattt tatacaatgc accaatcgcg cccgctgcca taaagtaacg ggtatagatg
     7381 tctgggctga ccgattcaat aaagtggtca tagtaagcca gcactgccgg aacgataccg
     7441 caggcaccgt tggttggcgc agttaccaca cgaccaccgg cggcgttttc ttcgttaact
     7501 gccagcgcaa acatgtttac ccagtcaatg acattcatcg gatcgttaga cagtttatcg
     7561 ctggaaacta acatccggcg cagggcagac gcacgacgtg gcacgcgcag cgggcctggc
     7621 agtacacctt cggtgttcat cccgcgatcg atacatgcct gcatggtttg ccagacatgc
     7681 gcgaaatact cgtcgatctc tttcttgctg tgcagcgcca gttcgttctg catagcgaga
     7741 ccagacagcg aatagccggt ttcattacag tacgcgagca gttcggtggc agatttgaac
     7801 ggatacggca cgctcacttc gttggcagca tcctgaccaa agtgttcttc atcgacgata
     7861 aaaccgccgc cgatggaata ataagttttg ctgtagacga cttcatcgcc gttataggcg
     7921 tggatttgca taccgttttc atgcagcggc aggttgccgt tatgaaaacg catcccgttg
     7981 tcgcgcggga aatccacttc atgccgtccc tgtgccagca gcagacgttc gcgctctttt
     8041 acgtcgcgaa taaaaccggg aatactgtcg atatccacgg tggcaggttc gttacctgca
     8101 agacccataa taatggcgat atcggtgtgg tggcctttac ccgtcagcga cagtgaacca
     8161 taaacgtcca cggcaacgcg agtaacgcta tccagtaagc ctttttcgac cagatcatcg
     8221 acgaactgtt tacccgcctt cataggccct acggtatggg aagatgaggg accaatcccc
     8281 accttaaaca tgtcgaatag actaatcacg ataatactcc tgacaaggtg actggacttc
     8341 cagtaacgaa ctaataacag cgcatagtgt aagagggaac gcggcgactg gcttaactat
     8401 tcacatgaat taaactaatc aaaaaccgcg atttaactaa tattgcgatg attgtctcaa
     8461 gtagtgtttt gtagatgtaa agataagtat agtcaggggg tcacaccaat ttgcagcgcc
     8521 agctcacgaa ttatgcctgc ggtcattccc catacaaaat actgttcgta ccaggacagc
     8581 catacccgat gtgaatcgcc acggcggtag atatctaaag ggtgataacg acccagatgt
     8641 aatgcctggg cgagcggcat ttcaaacacc gccgagactt catcttcact ggcgcgatac
     8701 ggcagatcgg gcgggataat gccgaccact ggggttactt gatagccagt tacgctatcg
     8761 acgggcggca gcacgccgat aacttcaacg gcggaaggcg gtatagcgac ctcttcttca
     8821 gcttcgcgca gcgcggcggc gataactgat gcgtccgtgt catcgactgc acctccaggg
     8881 aatgccactt gtccagcgtg tttacgcaga tgaattgaac gctgagtcag caacaacccc
     8941 ggttgcggtc gacggacgat ggggattaac acggcagcct gacgatgatt taggggttcc
     9001 cggttaattt gcgggcgcaa aagttgaaag cgagataaaa aatcatcaag cgtcaggcta
     9061 cggtattcca cgtcttactt ctccagttgg cgtaatatct tattaacttt atcaaaagtt
     9121 tcctgatatt ccgcttcttc ctggctatcg gcgacaattc caccgcccgc agagcagtat
     9181 atttgtccgt taatggcagt cagcgtgcgg atagtaatgc tggtatccat gttgccgcaa
     9241 aagctcaaat agccaatgct gccgcaccag gcattacgtc gctgcggttc cagttcgtcg
     9301 ataatttcca tagcccgtac tttcggagcc ccggttattg agccaccagg aaaagctgcg
     9361 cgcagcagat cgctggcgtg taactgttct ggtagtcgcg ccgttatagt gctgaccaga
     9421 tgatgcacgg cagggaaggg ttccaccacg aagagctctg gtacttttac cgaaccggct
     9481 acggcaacac gaccgatatc attacgcatt aaatcgacaa tcatcagatt ttcggcacga
     9541 tctttcgctg agttcgccag tttttctgct tgtttgctat cttcctgagg atcgggcagg
     9601 cgtggtagcg tgcctttaat cgggcgagtc tggatttcac tattatcaca aagaataaac
     9661 cgctctggcg aaaggcttaa aattgcaccc tgttcaagac gtaaaaaagc gctaaatggc
     9721 gcgcggttgg cctgattaag ctgaaggaat gcctgccatt catcgccaga ataggtcgca
     9781 tgaaaacgct gggcgagatt cacctgatag caatcaccgc tgtgcagata ttcctgtacc
     9841 tggcgaaatt tttcgccgta ctgctcgtgg gtcatattgg attgccagtc ggaagtgagc
     9901 gtgaaatctt cctgcggcga gaattgctgg ctttccagcc aggcccgacg ggcattgaca
     9961 tcattatgac tcagcaaaga aactgtatga cgctggtggt cgacaatgag cgcccaatcg
    10021 tagataccca ctgccatatc cggcagaacg atatcttgtt ccgcaatttc tggcagtgac
    10081 tcaaaacggc ggcccagatc gtagccaaac aaccccagtg cgccgccctg aaatggcaaa
    10141 tcttcgttat gcgttgggcg aatgtctgcg cgatccagca cctgctggag cacctgtagc
    10201 gggtcatcag tggtcgttgt gcgtttttcg ctttcactaa caacggtttc tttacctaaa
    10261 gtggttaaag tgcaaatcgg atcggcgacc acaatatcaa agcggctata cggatgatcg
    10321 gcatagccgg agtgtaaaag catcgcccac ggcaggtggc ttaagcggga gaaataaaat
    10381 tcagcggcgt cctgacgcca gggtaaagta atcacagtgg gagataacgt cttcatcagt
    10441 cctgactcta ctggctatgt gccgggtagc atgaaataat tagcgccaac aatttagcag
    10501 gagttaacaa tgtttgcagg tttaccttca ctcatccatg aacagcagca aaaagctgtc
    10561 gagcggatcc aggaactgat ggcacaaggg atgagtagcg gccaggcaat cgcgctggtg
    10621 gcagaagagc tgcgtgccaa ccactccggc gagctaatcg tggcgcgttt tgaggatgaa
    10681 gacgagtaac acactaatcc gtatcgattt cgttatagaa gccgatgatc atttgattgt
    10741 gagaaaaagg acgcttttta tatgtgatta aaagcgtcct gacccattta tgctgatata
    10801 aatttaactc agaccagtgc ttttagttct tcctgcatat atgttgaaaa gtattcagga
    10861 tttttaatca cagagcgttc acctgtctcc attttttttg cccatccaac cattttttcc
    10921 gtgaacccag ggttccagcc ttcctggtca gctcgcgccg taatatcagc ggcactcgcg
    10981 ggtactccca gttcttttaa atatttaaag attcccttcg ccgtgctttc attcatcgaa
    11041 cttggtgttg gcgctgatac atccatggca ccaatgaaat ccagggcttt atctattaca
    11101 gcaggcatat tcttatcctt aaaattaacc atgttatcga taaggctata tatgacctta
    11161 atttgcatct aatatcaaag ggattataac gatatggcac gagttaacct gttgacattc
    11221 aggtgtttta aaggtagcag ctcatggtgt gttatccagt aagatggaga aaattaacgg
    11281 tcagcccgca cgctataacc agattgttca gctaagaaag aaaaccagag ttgctaatca
    11341 aagggcgaac acaacggcat ggttatgttt atttatatag ttagttttat agataaaatt
    11401 gaaattataa atataaataa tgaattagca gaagcatcaa cagtaatatc ttattctctg
    11461 gtcgatttat gtaaaaccga ccagtattat cgataaatta tcgtctgact tattttgctt
    11521 cgataaagct tacaccgcag ccacaatctt aatttcaact ttatacttcg ggttcatcaa
    11581 acccgcttgt accgtgcagc gcaccggcgc atgacccgcg acaacccagg catcccacgc
    11641 tttattcatc gccgcgaagt cgtttttatc ggccaggaaa atggtggcat ccagaatgct
    11701 cgatttattg ctgccctgtt tttccagcac ggcgtcaatc tgtgccagcg tgttggcggt
    11761 ttgctcaaag gcatcggcgt cgaggttttc cggtacaccg gtgtagtaga gcgtgttgtt
    11821 gtggattact acatcagacc agcgggcttc agcatcgata cgaacgatag tcatcatttt
    11881 gtcctcatta tacttccatt ttcgtgctgc aaagactgcc acaatgtgag gttgccgtca
    11941 ccacttcaac tggcgaaagc gccctcatcc tgacataatc cctgttcaaa tcaacagggg
    12001 gtagtgtgac ggacgatttt gcaccagacg gtcagctggc gaaagcgata ccaggcttta
    12061 agccgcgaga accacagcga cagatggcgg tagccgtcac ccaggcgata gaaaaaggcc
    12121 agccgctggt ggtggaagca ggaaccggta cgggcaaaac ctacgcttac ctggctcctg
    12181 cgctgcgggc gaaaaagaaa gtcattatct cgaccggctc aaaagcgttg caggatcagc
    12241 tctacagccg cgatttgcca acagtctcaa aggcattgaa atatacgggc aacgtggcgc
    12301 tgctgaaagg gcgctcaaac tacctctgcc tcgaacgtct cgaacagcag gcgctggcgg
    12361 ggggcgatct gccggtacaa atcttaagcg atgtgatcct gctgcgctcc tggtctaatc
    12421 aaacagtcga tggtgatatc agcacctgcg tcagcgtggc ggaagattca caggcgtggc
    12481 cgctggtcac cagcaccaac gacaactgtc ttggcagcga ctgcccgatg tataaagatt
    12541 gctttgtggt caaagcacgt aaaaaagcga tggacgccga tgtggtggtg gtaaaccatc
    12601 atctctttct ggcggatatg gtggttaaag agagtggatt tggcgagttg atccctgaag
    12661 cggacgtcat gatcttcgac gaagcccacc agctaccgga cattgccagc cagtattttg
    12721 gtcagtcact ctccagtcga caactgctcg acctggcaaa agacatcacc atcgcctacc
    12781 gcaccgaatt aaaagacacc cagcagttac aaaagtgcgc tgatcgtctt gcccagagtg
    12841 cgcaggattt tcgtctgcaa ctcggtgagc caggttatcg cggtaacctg cgtgagctgt
    12901 tagctaatcc gcaaattcag cgggcatttt tactgctcga tgacaccttg gaactttgtt
    12961 atgatgtggc gaaactgtca ctggggcgtt ccgccttgct ggatgcggca tttgagcgcg
    13021 ccacgttgta tcgcacacgg ctgaagcggc taaaagagat caatcagccg ggctacagct
    13081 actggtacga atgcacttcg cgccatttta ctctggctct cacgccgctc agcgtggcgg
    13141 ataaattcaa agagttaatg gcgcaaaagc ccggtagctg gatcttcacc tcagcaacgc
    13201 tgtcggtgaa cgacgatctg catcatttca cctcgcggct tgggatagaa caggcggagt
    13261 cgttgctatt gccaagcccg tttgattaca gccgccaggc gttactctgt gtgccgcgca
    13321 atctgccgca aaccaaccag ccaggttctg ctcgccagtt agcggcaatg ctgcgaccga
    13381 tcatcgaagc taacaacggt cgttgtttta tgctttgtac ctcgcacgcc atgatgcgcg
    13441 atctggccga gcagttccgc gctaccatga cgcttcccgt tttgttgcag ggggaaacca
    13501 gtaaaggcca actgttgcag cagtttgtca gtgccggtaa tgcgcttctt gtggcaacca
    13561 gtagtttctg ggaaggggtg gacgtgcgtg gcgatacatt gtcattggta attatcgaca
    13621 aattgccgtt tacctcgccg gatgatccac tgttaaaagc gcgcatggaa gattgtcgtt
    13681 tgcgcggtgg cgacccgttc gatgaagtgc aactaccaga tgccgtcatt actctcaaac
    13741 agggggtagg tcgactgatt cgcgacgccg acgatcgtgg cgtgctggtg atttgtgact
    13801 atcggctggt gatgcgtcct tacggcgcga cgtttctcgc cagtctgccg cccgcgccac
    13861 gcacccgtga cattgcccgt gcggttcgtt tccttgcgat accatcctcc aggtaatttg
    13921 ttacggagta tggtatgatg cgcgcctttt tattgatcta agcacgaaga cccatgcgaa
    13981 ttctggctat cgataccgcg acagaggcct gctctgtcgc cctgtggaac gacggtactg
    14041 tcaacgctca ttttgagctt tgccctcgtg aacatactca acgaatctta ccgatggtgc
    14101 aggatatcct gaccaccagc ggaacttccc tgactgatat taacgctctg gcttacgggc
    14161 gcggccccgg tagctttacc ggcgtgcgca ttggtattgg catcgcgcaa gggctggcgc
    14221 ttggcgcgga attaccgatg attggcgtct ccacgctaat gacgatggcg caaggggcgt
    14281 ggcgcaaaaa cggcgcaaca cgcgtgctgt cagccattga tgcgcgaatg ggcgaagttt
    14341 actgggccga atatcagcgt gatgaaaacg gtatctggca cggtgaagaa accgaagccg
    14401 tactcaaacc cgaactcgtt catgaacgaa tgcaacagct ttccggcgaa tgggtgacgg
    14461 tagggacggg ctggcaagcc tggccggatc tcggtaaaga gagcgggctg gttttgcgcg
    14521 atggcgaagt gttactcccc gctgcagaag atatgctgcc gattgcttgt cagatgtttg
    14581 ccgagggtaa aacggtggcg gtggaacatg ccgagccggt ttatttgcgt aacaacgtcg
    14641 catggaagaa acttccgggc aaagaatgaa tcttaagagt atgccctgag aaaaaaagga
    14701 gtcgcatcat ggcggttcaa aagaatgtta tcaaaggcat actggcaggt acgtttgcgc
    14761 taatgctgag cggttgtgtc actgtgccgg acgccattaa aggcagcagt accacgccgc
    14821 aacaagattt agttcgggtg atgagtgcgc cgcagctgta cgttggtcag gaggcacgct
    14881 ttggtggcaa agtggttgcg gtacaaaacc agcaagggaa aacccgcctg gaaattgcta
    14941 ccgtaccgct ggacagcgga gctaggccga cgctgggaga accttctcgc ggtcgcattt
    15001 atgccgatgt gaacggtttt ctggacccgg tggatttccg tggacaactg gttacggtag
    15061 tcgggccaat caccggtgcg gttgacggca aaatcggcaa tacgccctat aaatttatgg
    15121 tgatgcaagt aacgggttac aaacgttggc atttaaccca gcaggtgatt atgccgcctc
    15181 agccgattga tccatggttt tatggcggtc gtggctggcc ctatggctac ggcggatggg
    15241 gctggtataa tcccggcccc gcgagagtac aaacagttgt aaccgaataa ttgcttgttt
    15301 ttaaagaatg cagaaacagc ggctggtccg ctgtttctgc attcttacgg taaagataaa
    15361 aataaatagt gacgcgcttc gcaacctttt cgttgggtaa ttatcaagct ggtatgatga
    15421 gttaatatta tgttaacggc gtgtatatca tttggggttg cgatgacgac gaacacgcat
    15481 tttagaggtg aagaattgaa gaaggtttgg cttaaccgtt atcccgcgga cgttccgacg
    15541 gagatcaacc ctgaccgtta tcaatctctg gtagatatgt ttgagcagtc ggtcgcgcgc
    15601 tacgccgatc agcctgcgtt tgtgaatatg ggggaggtga tgaccttccg caagctggaa
    15661 gaacgcagtc gcgcgtttgc cgcttatttg caacaagggt tggggctgaa gaaaggcgat
    15721 cgcgttgcgt tgatgatgcc taacttattg caatatccgg tggcgctatt tggcattttg
    15781 cgtgccggga taatcgtcgt aaacgttaac ccgttgtata ccccgcgtga gcttgagcat
    15841 cagcttaacg atagcggcgc atcggcgatt gttatcgtgt ctaactttgc ccacactctg
    15901 gaaaaagtgg ttgataaaac cgccgttcag cacgtaattc tgacccgtat gggcgatcag
    15961 ctatctacgg caaaaggcac ggtagtcaat ttcgttgtta aatacatcaa gcgcttggtg
    16021 ccgaaatacc atctgccaga tgccatttca tttcgtagcg cactgcataa cggctaccgg
    16081 atgcagtacg tcaaacccga actggtgccg gaagatttag ccttcctgca atacaccggc
    16141 ggcaccacag gtgtggcgaa aggcgcgatg ctgactcacc gcaatatgct ggcgaacctg
    16201 gaacaggtca acgccaccta tgggccgttg ctgcatccag gtaaagaact ggtagtaacg
    16261 gcactgccgc tgtatcacat ttttgccctg accattaact gcctgctgtt tatcgaactg
    16321 ggtgggcaga acctgcttat cactaacccg cgtgatattc cagggttggt aaaagagtta
    16381 gcgaaatatc cgtttaccgc tatcactggc gttaacacct tgttcaatgc gttgctgaac
    16441 aataaagagt tccagcagct ggatttctcc agtctgcatc tttccgcagg cggtgggatg
    16501 cctgttcagc aagtggtggc agaacgctgg gtgaaactga ccggacagta tctgctggaa
    16561 ggttatggcc tgaccgagtg cgcgccgctg gtgagcgtta acccgtatga tattgattat
    16621 catagcggta gcatcggttt gccggtgcca tcgacggaag ccaaactggt ggatgacgac
    16681 gacaacgaag taccaccggg tcaaccgggt gagctttgtg tcaaaggacc gcaggtgatg
    16741 ctgggttact ggcagcgtcc ggatgctaca gatgagatca tcaaaaatgg ctggttacac
    16801 accggcgaca tcgcggtgat ggatgaagaa ggattcctgc gcattgtcga tcgtaaaaaa
    16861 gacatgattt tggtttccgg ttttaacgtt tatcccaacg agattgaaga tgtcgtcatg
    16921 cagcatcctg gcgtacagga agtcgcggct gttggcgtac cttccggctc cagtggtgaa
    16981 gcggtgaaaa tcttcgtagt gaaaaaagat ccatcgctta ccgaagagtc actggtgact
    17041 ttttgccgcc gtcagctcac gggatacaaa gtaccgaagc tggtggagtt tcgtgatgag
    17101 ttaccgaaat ctaacgtcgg aaaaattttg cgacgagaat tacgtgacga agcgcgcggc
    17161 aaagtggaca ataaagcctg agcgttaagt cagtcgtcag acgccggtta atccggcgtt
    17221 ttttttgacg cccactaaag agaaaacaat ttgaattacc aaatgattac cacggacgat
    17281 gcgctggctt ctttgtgtga agccgtccgt gtctttccgg cgatagccct ggatactgaa
    17341 tttgttcgta cgcgcactta ttacccgcag ctggggttga ttcaactttt cgatggcgag
    17401 catctggcgc taattgatcc tctcgggatc accgactggt caccgctgaa agcgatcctg
    17461 cgcgatccgt ccatcacaaa atttctccat gcaggcagtg aagatctgga agtgttcctc
    17521 aatgtctttg gcgaattacc acaacccttg attgacacgc aaatccttgc tgccttctgc
    17581 ggacgcccga tgtcatgggg tttcgcttcc atggtggaag agtattccgg cgttacgctg
    17641 gacaagagtg aatcgcgcac cgactggctg gccagaccgc tgaccgaacg tcagtgtgaa
    17701 tacgcagcgg cggatgtctg gtatctgtta ccgatcaccg ccaagcttat ggtagaaacg
    17761 gaggcctccg gctggctacc tgcggcgctg gatgaatgcc gcctgatgca aatgcgtcgt
    17821 caggaagtcg ttgcgccgga agatgcctgg cgtgatatca ccaatgcctg gcagttacgc
    17881 acacgccaac tggcctgtct gcaactgtta gccgactggc gactgcgcaa ggcgcgagag
    17941 cgtgatctgg cggtgaactt tgtcgtgcgt gaagagcatt tgtggtcggt cgcgcgttat
    18001 atgccgggaa gtttaggcga actggacagc ctgggtttat ccggtagcga aatccgcttt
    18061 cacggtaaaa cgctgctggc gctggtggaa aaagcgcaga cattgccgga agatgcctta
    18121 ccgcagccga tgcttaacct gatggacatg cctggttatc gtaaagcgtt taaagcgatt
    18181 aagtcgctga ttactgacgt gagcgaaacg cataagatca gcgccgaatt gctggcatcg
    18241 cgtcggcaaa tcaaccaact gctgaactgg cactggaaac tgaaaccgca gaacaatttg
    18301 ccggagctga tttccggctg gcgtggtgag ctgatggcgg aagcattaca caatttattg
    18361 caggaatatc cgcagtaaaa tcttccgaag ccggactggg cgcgctcagc gccacatccg
    18421 gctttggcaa actacaaatc caacaccagg cgtttaccct tcgcacgcga acaacagatc
    18481 aacatacttt gctggctggc acgctcttca tcgctaaaat actgatcccg atggtcagct
    18541 tcaccttcca gtattgccgt ttcgcaggtt ccgcataccc cttcacgaca taaacattcc
    18601 actttcgcgg ctttattatt ttcaataacc tgcaaaatag tcatctcttc cggcaccaca
    18661 aactcttttc cggaacgggc aagcaccagg gtaaatgcat cgccggtttt gtcttcgata
    18721 gcaaattgct caaagtgcag tgtatcggcg gcgatgtcca gacgcgcagc ttcacttctt
    18781 accgcttcaa ttagcgcctc ggggccacag gtataaacgt gtgtgccagg ttcgatatcc
    18841 gccaataatc gcgctaattc cagtcgtgtt ccggttgatg aatgatgcaa atggactttc
    18901 tcagcctgcg gatgctggac tagctcagca cgaaatgcgc aactttctgg atttcgcgag
    18961 cagtaatgta gctgccagtc gatgtcgctg tgttgcaact ctgccatgtg cgacaggaaa
    19021 ggggtgatac caataccgcc cacgataaac agatgctttc tggctgaggg aattagcgca
    19081 aacaggttat taggcgttga gatcgttaac cgatcgccca cttttacctg ctgatgcaaa
    19141 aagcgggaac cgccgcgcga gttttcttcc agtcgaacgg caatctgata actagaggtg
    19201 ttatgcggtg aactcagtag tgaatacgca ttgctgtact ggttatcacc atcgctcatc
    19261 tgcactagga tgtgacttcc tccggtgaac gcaggcagcg gtttgccatc ggttgccacc
    19321 agcgtgaagc gtttcacctg ttcggtaagg ggttcaacct ggctcacctg tacttcaaac
    19381 atttgatagt ctgacattgc tgtactccat caaaaatcag cccagcggcg gaaaataccg
    19441 ccgccgatgt cattagtcct taaacacctg cgccagcaga ttatggaaat gggcgatacc
    19501 atgttcggaa atgccactac cgctactgtc ggccatgatg cgcccctgac cacgatagcc
    19561 acgcgatttc agccctttct gtacgctttc aaccagacgt aaatcttccg gatggaacac
    19621 atcgcgatac cactcaatca gcgatttttg ctcgtcggtt aactcttcat tggtgaagta
    19681 aatatcgtag ttttgcaggg tagtttcaga atccaccggg aattcataaa tgaccgtcat
    19741 catccctttg atcggggtga cgttcagcat cgtgcacggc cacagccaga aaccgtggaa
    19801 tgccgcatcc gtaccctctt caaatttaaa cgactgttcg gacggtttgg cgaaaccgta
    19861 ttgcagcgtc cagttaccgt gcatggtgtg ccagtaacga tcaacctgta cggagtcgga
    19921 gaaacctaga tgcgccggac cacagtgata gcactcgaga tagttatcga caatgttctt
    19981 ccagttggca ggcgtgcggg tggtaaagcg ggccgctagt ttcagattgt ggacttccgg
    20041 gcaggcttcc agcactttcg cgcccagacc ggtgtattga ttcacttgaa gtacgaaaaa
    20101 aaaccgggag gacattggat tattcgggtt ctgatgggat tagatttggt ggggcttgca
    20161 agcctgtagc gcaaatttta gtcgttaatc aatgaaacgc gaaagatagt aaaaaattgc
    20221 ttttgtttca ttgaaaatac gaaaaacaaa aacactgcaa atcatttcaa taacagcttc
    20281 aaaaaacgtt caaaaccgat aacaaccagg ctgtaaccaa atgactcata tcacaaatca
    20341 gctcatgccg tttaggtatg ttacatgtgt gattatgtga ggtgaagtat gttttagctg
    20401 gttcatggtt gttatacggc tttttttaca tcttgtggtt cttgtgaagg agctacaaga
    20461 ccttcctgtt cataaatacc atactttgac aaaatctttt tacgagtatc tgttggtaat
    20521 gattcaagca ttttggctgt ttgtaaggtt tgctgcgaaa taccttgcaa atcagcctgg
    20581 atggaatcat cggaagaaaa aatctcaggc ataagagctt caaatccttt ctttctgaac
    20641 aactcaatcg cagaatgcag ttccttgtcc tctaacaatt cgacaattgc gatcagttta
    20701 gttttcaatg tgctgcggtc aggttgagaa tttttattct cttgattatt tttatgctca
    20761 accattggca tgctctgact ttggataaac cactctattg gacgccctgt tgcgtaggct
    20821 atttgtgcta atcgatctaa agacgggtat gtcttgccag acaggtagtt tctgataacc
    20881 ccatcagaaa gtccgcactt agctgcaaac gcccggcttg attctccatt cattgcctct
    20941 tcaaggcgtt ccttgaaacg cattatttcc gggggcataa aaatgcgctc tgcatttttg
    21001 ctttcttttt cacctgtcat gcccaatctc tctgttatat aaagaatttt acagaattct
    21061 ttgatttgca ctggttaaaa taaaaatgcg catagctttt gaattgagca tttttctgcg
    21121 ctatcgtttt tcccgatagc ggtagccggt atcagttacc gaaaacgtta agagagtaag
    21181 gatcgcagaa tgtcagcaaa aaaccaagac tggcacgcag agcagataaa ggctgctgtt
    21241 cgtatgaagg ggaagacact atctcaatta tccagag
//
LOCUS       DAJTUI010000066        21066 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTUI010000066 DAJTUI010000000
VERSION     DAJTUI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.066"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..604
                     /locus_tag="OO464_002669"
                     /pseudo
     CDS             1..604
                     /locus_tag="OO464_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="TPM domain-containing protein"
     gene            complement(664..1962)
                     /gene="eno"
                     /locus_tag="OO464_002670"
     CDS             complement(664..1962)
                     /gene="eno"
                     /locus_tag="OO464_002670"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6227768.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2050..3687)
                     /gene="pyrG"
                     /locus_tag="OO464_002671"
     CDS             complement(2050..3687)
                     /gene="pyrG"
                     /locus_tag="OO464_002671"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6227769.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3915..4706)
                     /gene="mazG"
                     /locus_tag="OO464_002672"
     CDS             complement(3915..4706)
                     /gene="mazG"
                     /locus_tag="OO464_002672"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6227770.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4802..7036)
                     /locus_tag="OO464_002673"
     CDS             complement(4802..7036)
                     /locus_tag="OO464_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR6227771.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7084..8385)
                     /gene="rlmD"
                     /locus_tag="OO464_002674"
     CDS             complement(7084..8385)
                     /gene="rlmD"
                     /locus_tag="OO464_002674"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6227772.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8442..11198
                     /gene="barA"
                     /locus_tag="OO464_002675"
     CDS             8442..11198
                     /gene="barA"
                     /locus_tag="OO464_002675"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6227773.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11430..12770)
                     /gene="gudD"
                     /locus_tag="OO464_002676"
     CDS             complement(11430..12770)
                     /gene="gudD"
                     /locus_tag="OO464_002676"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6227774.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12791..14131)
                     /locus_tag="OO464_002677"
     CDS             complement(12791..14131)
                     /locus_tag="OO464_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6227775.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14133..15485)
                     /gene="gudP"
                     /locus_tag="OO464_002678"
     CDS             complement(14133..15485)
                     /gene="gudP"
                     /locus_tag="OO464_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6227776.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15919..16368)
                     /locus_tag="OO464_002679"
     CDS             complement(15919..16368)
                     /locus_tag="OO464_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6227777.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16386..17168)
                     /gene="truC"
                     /locus_tag="OO464_002680"
     CDS             complement(16386..17168)
                     /gene="truC"
                     /locus_tag="OO464_002680"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6227778.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17168..17497)
                     /locus_tag="OO464_002681"
     CDS             complement(17168..17497)
                     /locus_tag="OO464_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6227779.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18119..18664)
                     /gene="syd"
                     /locus_tag="OO464_002682"
     CDS             complement(18119..18664)
                     /gene="syd"
                     /locus_tag="OO464_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6227780.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18732..19580
                     /gene="queF"
                     /locus_tag="OO464_002683"
     CDS             18732..19580
                     /gene="queF"
                     /locus_tag="OO464_002683"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6227781.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDKDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19692..21056
                     /gene="ppnN"
                     /locus_tag="OO464_002684"
                     /gene_synonym="ygdH"
     CDS             19692..21056
                     /gene="ppnN"
                     /locus_tag="OO464_002684"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6227782.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5157 a         5629 c         5039 g         5241 t
ORIGIN      
        1 aagagttttt gacaattggc gtttaggaga tgccaaacgt aatgatggga tactgatcgt
       61 tgttgcctag tcggatcgca ctgtccgcat ccaggtaggt tatgggctgg aagaaaaggt
      121 aaccgatgct ctggctggag atatcatccg tagcaacatg atacccgcat ttaaacaaca
      181 aaaattagct aagggattag agttagctat aatcgctttg aataaccaac tcacttcaca
      241 acaccaatat ccggctaatc cttcagaaag tgaatcaacg tcttccactg atcattatta
      301 ctttgctatt ttttgggtat ttgcagtgat gttcttccct ttctggtttt ttcatcaagg
      361 cagtaatttt tgtcgcgcat gtaaaagtgg agtctgtatt tcagcaatcc atcttttaga
      421 tttattcctg ttctccgata aaactttttc cattgctgta ttttccttct tttttacttt
      481 caccatattt atggtcttta cctgtttatg tgtacttcag aaaagagcat ctggtagaag
      541 ttatcattca gacaacagcg gttccgctgg aggctcaagt ggcggcgcat ccggccgctg
      601 gtaactccac cataaaaaat gccagcccga aggctggcat tatcaaatca gataaagtca
      661 gacttatgcc tggcctttga tctctttacg accgttgtac ggtgcttttt cgcccagagc
      721 ttcttcgata cgaatcagct ggttgtattt agcaacacgg tcagaacggc tcatagaacc
      781 agttttgatc tggcctgcag cagtaccaac agccagatca gcgatggtag catcttcagt
      841 ttcaccagaa cggtgagaga taactgcagt gtagccagca tctttcgcca tcttgattgc
      901 agccagagtt tcggtcagag aaccgatctg gttgaatttg atcaggatgg agttagcgat
      961 acctttttcg ataccttctt tcaggatctt ggtgttggtt acgaacaggt cgtcaccaac
     1021 cagctggatt ttgtcgccca gaactttggt ctggtatgcg aaaccgtccc agtcagattc
     1081 gtccagaccg tcttcgatag aaacgatcgg gtactgtttg gtcagttctt ccaggaagtg
     1141 agtgaattct tcagaggtga acgctttgtt gccttcgcca gccagaacgt atttaccatc
     1201 tttgtagaat tcagaagctg cgcagtccat cgccagagtg atgtctttgc ccagttcata
     1261 accagcagct ttaacagctt cagcgataac agccagagct tcagcgttgg aacccaggtt
     1321 cggcgcatag ccaccttcgt caccaacagc agtgttcatg cctttcgctt tcagaacttt
     1381 tgccaggtga tggaaaactt cagaacccat gcggatggct tctttcacag ttttcgcgcc
     1441 aaccggctga atcatgaatt cctggatatc aacgttgttg tcagcgtgct caccaccgtt
     1501 gatgatgttc atcatcggaa ccggcatgga gtatttgccc ggagtgccgt tcagttcagc
     1561 gatgtgctcg tacagcggca tacctttagc agctgcagca gctttggcgt tagccagaga
     1621 tacagccagg attgcgttcg cgccgaattt ggatttgttt tcggtgccgt ccaggtcgat
     1681 catgatcttg tcaatgccag cctgatcttt agcatctttg ccaatcagcg cctgagcgat
     1741 cgggccgttt accgcagcaa cagctttggt tacgccttta cccaggaaac gggatttgtc
     1801 gccatcgcgc agttccagag cttcacggga accagtagaa gcacctgacg gagcagctgc
     1861 cataccgacg aaaccaccct ccagatgtac ttcggcttca acagtcgggt taccacggga
     1921 gtcgatgatt tcacgaccga tgatttttac gattttggac attaggtttt cctcaagtca
     1981 ctagttaaac tgaaactcca gacaaacaac gcgtacccaa ggtacgcgtt gccgttctaa
     2041 cttttttact tacttcgcct gacgtttctg gaactcgctg gcggctttca caaagcctgc
     2101 aaacagcggg tgaccatcac gtggagtaga agtaaactcc ggatggaact ggcaagccac
     2161 gaaccacggg tgattcggaa cttcgatgat ctcgaccaac tgatcatccc cggaacggcc
     2221 cgcaacgcgc agacctgcat cttcaatctg tttcaacagc atgttgttga cttcgtaacg
     2281 gtgacgatga cgctcaacaa ttgtcggcgc attgtatagc tggcgaacca ggctatcgtc
     2341 aaccaactgg cactgctgtg cgccgagacg catggtaccg ccgagatcgc tcttctcgct
     2401 acgaacttca acgttgccgt tttcatcgcg ccactcggta atcagcgcca caaccgggta
     2461 cttacagtct ggcacaaatt ctgtagagtt ggcgttctcc atgttggcaa catggcgagc
     2521 gtaatcaatt aacgccacct gcatacccag gcaaatgccc agataaggaa tattgttctc
     2581 acgcgcaaaa cgcgcggtcg taatcatgcc ttctacgcca cgatagccga aaccgccagg
     2641 tacgaggatt gcgtccagac ctttaaggat ttcaacgccg cgcgtttcaa catcttgtga
     2701 atcgatcagt ttgatgttga cgctgacacg attcttcagc ccaccgtgtt tcagtgcttc
     2761 gatcactgat ttataagcat ccggcagttc aatgtacttg ccgaccatac cgatggtgac
     2821 ttcacttacc ggattcgctt cttcgaagat aacctgttcc cattcggaca gatttgcttc
     2881 cgggcagttt aagctgaatc gtttacaaat ataatcgtcc agcccctgag atttcaacag
     2941 gcccggaatt ttatagatgg aatcgacgtc tttcagagaa ataaccgctt tttccggaac
     3001 attacagaac aatgcaatct tcgcacgttc gttcgccggt acagcgcgat ctgaacgaca
     3061 aatcaggatg tcaggctgga taccgatgga gagcagctct tttacagagt gctgagtcgg
     3121 tttggttttg acttcaccag acgctgccat gtacggcacc agcgtcaggt gcataaacag
     3181 agtgtgctca cggccaattt caacagccat ctggcgaata gcttcgagga acggcaagga
     3241 ttcgatatca cctactgtac cgccgatttc taccagtact acgtcatgac cttcgccacc
     3301 ttccagcacg cgctctttga ttgcgttagt gatgtgcgga ataacctgca cggttgcgcc
     3361 gaggtagtca ccgcggcgtt ctttacgcag aacgtcagag tagatacgac ccgtggtgaa
     3421 gttgttgcgg cggctcattt tggtacgaat gaaacgctcg tagtgcccca ggtccaagtc
     3481 ggtttcagcg ccgtcttcag taacgaacac ttccccgtgt tggattgggc tcatagtacc
     3541 tggatcgacg ttgatgtaag gatccagttt catgatggtc acattgaggc cacgggcttc
     3601 aagaatggct gcgagggagg ctgcggcaat gcctttaccc agagaggata cgaccccgcc
     3661 ggtcacaaaa atatagttcg ttgtcatgct gaacctgaga agttaggttg aaaagacgat
     3721 ggaataacca agacgggaaa gcagtatacc cgaacatgac ctgtgccaca aactttcatt
     3781 atccctcctc ttcgccagcg cactattgaa atcaggagtg agaaaatagc ccctttgggg
     3841 taaatgtttt tgacgcaaat caagcgcttg tcatttaaaa aatgacacaa atggcgcttg
     3901 accgcgtaat tcccttagag atcaatttcc tgccgtttta cctgttgcca gacttcttcc
     3961 attgtttcga ggtcaacacc tgtcatttcc agtccacgcg cggcaacaat acgctccact
     4021 tcgcgaaaac gacgctcgaa tttttcgttc actttttgca atgcgatttc tgctttcgtc
     4081 cctaaatggc gagccagatt aaccgtggca aacagcaggt cccccatttc ctcctccagt
     4141 ttagcctggt cgacaacagc ctgccgcgcc tcgtacatca cctcgtcgat ctcttcgtag
     4201 actttatcaa ccaccgggcc aagcgtcgtc cagtcgaagc caacgttggc gcaacgtttc
     4261 tggatttttt gcgcccgcat taaagccggt aaactacgag gaatatcgtc cagcgccgaa
     4321 tgctgcgctt tctgcgcgcg ctcttcggtt ttgatttgct cccaacgggc aagcacttca
     4381 ctactgtttt cggcagaact atcagcaaaa acatgcggat ggcgacgctc taatttatcg
     4441 ctaatagcag cgcaaatatc attaaagtca aagcgccctt cttcctgagc catttgcgcg
     4501 taaaacacca cctggaatag cagatcgccc agttcaccgc gaagatcgtc aaaatcttca
     4561 cgggcgatgg cgtccagcac ttcgtaggtt tcttcaaggg tgtaaggcgc aatggtggca
     4621 aatgtctgct ctttatccca cgggcagccg ttttccggat cgcgcaggcg ctgcataata
     4681 gtgagcaaac ggtcgatttg attcattgaa ttgtcctgaa aattgcgggt ctgtcaggtg
     4741 taataccttc gagtaaaaga tatcgctgga tacggctgac gccatatcca gctatgtaag
     4801 actaactccc gtgcaaccga cgcgcgtcga taacatccgg cacctggttg agtttaccca
     4861 gcacgcgccc cagcacttgc aggttgtaaa tctcaatggt catgtcgatg gtcgccagtt
     4921 gctgtttggt gtcgctacgg ctggcaacgc caagcacgtt caccttctcg ttggcgagaa
     4981 tggtcgtgat atcacgtaac aacccactac gatcattagc caccacgcgg accaccagcg
     5041 aatatccggc ggagtagctc tcaccccata ccgcgtcaac aatgcgttct ggcgcatggg
     5101 agcgcagttc cgccagttgt tcgcaatcgg cgcggtgtac tgaaataccg cgtccctggg
     5161 taatgaagcc gacaatctca tctccaggaa tcggctggca gcagcgcgcg atgtggtgca
     5221 tcaggttacc aacaccttca accaccacac gaccgttatc tttactgcgg ttttgcggtg
     5281 tgtagctttt ttgctgaagc tgtttcagcg cggcggcgtc ctgctcttcg gcactcggct
     5341 tattaaattg cgattgcagg aagttcacca tctgattgag acggatatcc ccgccaccaa
     5401 tcgccgccag caactcgtcg acatcattga agttgtaacg cggcagcaga tgtttttctg
     5461 cttctttcag gctgatcccc agatgttcca gctcgtcgtc aaggatttgc cgcccagcca
     5521 gaatgttttt gtcacggtcc tgtttacgga accaggcgtg aattttcgaa cgcccacggc
     5581 tggttgtgac gtaaccgagg tttgggttta accagtcacg gctggggttc ggctgtttct
     5641 gggtgataat ttcaatctgg tcgcccattt gcagctggta ggtgaacggc acaatgcgcc
     5701 cgccaatttt tgccccgatg cagcggtgtc cgacatcact gtggatgtgg taagcgaagt
     5761 ccagcggcgt tgatcccgca ggcaaatcaa cgacatcacc tttcggcgta aagacataca
     5821 cccggtcgtc aaacacctga ctacgtactt cgtcgagcat ttcgccggaa tcagccatct
     5881 cttcctgcca cgcaatcagt ttacgcagcc aggcaatccg gtcttcatgt cccgaacgtg
     5941 cgccgccagc tgccgcgccc tctttatatt tccagtgcgc agcaacaccc aactccgcat
     6001 cttcatgcat ctgtttggtg cggatttgga tctcaacggt ttttccaccc ggccccagaa
     6061 ccacggtatg aatagactga taaccgtttg gtttcgggtt agcgacgtaa tcgtcaaact
     6121 catccggcag gtggcgatag tgagtgtgca ctatccccag tgcggcataa caatcctgta
     6181 aacgctcggc gacaatacgt accgcacgca catcaaacag ctcatcgaag gcgaggttct
     6241 ttttctgcat tttgcgccag atgctgtaga tgtgtttcgg acgaccatac acttcagctt
     6301 taacgccttc agctttcatc tcagcgcgca gatggccgac aaactcttcg atatagtgtt
     6361 cgcggtcgag acggcgttca tgcagcagtt ttgcgatgcg tttgtattcg gtcgggtgga
     6421 gataacggaa gcagtaatct tccagctccc atttcagttg cccaatccca agacggttcg
     6481 ccaacggcgc gtagatattg gtgcactctt ttgccgccag tacacgttca tcttccggcg
     6541 catcttttac ttcgcgcaga tgagcaatac gctccgccag tttgatgact acgcagcgaa
     6601 aatcatcgac catcgccaat aacatccggc gaacgttatc gacctgttcg gaggaaacag
     6661 aatcagtgtg cgtcgctttc agctggcgga tcgccgccat atcacgcacg ccgtgaataa
     6721 ggttaacaac cgacttaccg acgctctcac gcagcacatc ttcgctgact acgttggcat
     6781 cagccagagg gaacagcagc gccgcccgca gcgtgtcaat gtccatactt aatgtcgaga
     6841 ggatctccac catctcaaca ccacgccaca ataacagact ggcatccgga tgcccctgcg
     6901 tctgttgcag acaatacgcc caggtttcgg ctaagcactc acacgacttc tggctggtaa
     6961 tacccagact tgcgatccat ttttccggat caaattcacc agccttattg atatgtgcac
     7021 ttcttaccgc aaccatcgtc ctctccttta gggaccagac ctgccgaaat cggcaaatcg
     7081 taactattta acgcgcgaga aaagtaccat cgattccaga tgtcccgtgt gtgggaacat
     7141 atccagcatc gccagtcgcg caatggtata tcctgctttt aataacgctt cgctatcccg
     7201 agccagcgtt gcagggttac aggatacata aactatacga ataggttcca gttttataat
     7261 ttgctgcata acacctgcgg cacctgctcg cgccgggtcc agcaacactt tatcgaagcc
     7321 gtttttcgcc cacggctgct ttgtgacatc ttcttcaaga ttttcgtgat aaaacgtcac
     7381 attctgtaag ccattaagac gcgcattctg ctggcctttt tccaccagcg caggaacacc
     7441 ttctacaccg accacactgg cagcttgtgt cgccaatggc agtgtaaagt tgcccatacc
     7501 gcagaacaga tccagtacgc gatcttcagg ttgcacatcc agccattcca acgcacgcgc
     7561 taccattttt tggttcacac ccgcattgac ctgaataaaa tcgcgcgggc taaaagttaa
     7621 gcgcaacccg tttgagtcat accagggcat ctcaccagag acggtttcga gtatctcact
     7681 atcgggggcg agatacagat ccaggccttc agaatgcgaa aagcgttcca gtttttcgcg
     7741 atctgccgaa cttagcggtg cggtatggcg caaaatcatc agcgtgccgc tggttgcctg
     7801 taccagttca acatgaccaa gatggcgcat agcttgtaag ctgcccagac atgccctgac
     7861 tttgggcagc aatgcttcaa gttggggcgc taaaatgggg cattgtttga cgtcgacaat
     7921 gtcactggag cccgctttgc gaaaccccat ctgaagttgc tgtgttttcg gtaagtagtt
     7981 cagacttaaa cgcgcgcggc ggcgatagcc ccagggaaca tcggcgatca cttcagagac
     8041 atcgtgtttc attaatcggg cgagtgccgc acttttgctt cgctgctgta aatccacgct
     8101 ggcgtgttgt tgctgacagc cgccgcatac gccaaaatga ggacagcgtg gcgtttcgcg
     8161 ttccgggcta tcgcttaacc ggcgtacgac tttagcgcgg gcatactgtt ttttatcttc
     8221 agtaacagca acttccgcgt tttcctgcgg caataatccg gggataaata acgttttgcc
     8281 gttatgtcgc gccacgccct gaccaaaaga gtcgaggtcg ttgactgaaa cggttatgat
     8341 ctgacgcgtc gtcgtgcgtc gttttgcaga gtagaattgc gccattggcg agactttctc
     8401 aatttaacag tgtgacctta attgtcccat aacggaactc catgaccaac tacagcctgc
     8461 gcgcacgcat gatgattctg atcctggcac cgaccgtcct tattggttta ttgctgagta
     8521 tctttttcgt cgtgcatcgc tataacgact tgcagcgtca actggaagat gccggtgcta
     8581 gcattattga gccgcttgca gtttctactg aatatggcat gagcctgcaa aatcgcgaat
     8641 ctatcggtca gttaataagc gtactgcatc gtcgccattc cgatattgtt cgcgcgattt
     8701 cggtttatga tgaaaataac cgactctttg tcacctccaa ttttcatctt gatccctcat
     8761 caatgcagct cggcagcaac gtgccgtttc ctcgccagct cactgtcact cgtgacggcg
     8821 atattatgat cctccgcacg ccgattattt ctgagagtta ctcccccgac gaatcgccca
     8881 gtagcgatgc caaaaatagt caaaatatgt tgggatatat tgcgcttgag ctggatctta
     8941 aatcggttcg cttgcagcaa tataaagaga tctttatttc ctgcgtgatg atgctgtttt
     9001 gtatcggtat tgcgcttatt tttggctggc gcttaatgcg cgatgtaacc ggtccgattc
     9061 gcaacatggt gaataccgtc gaccgcatcc gtcgcgggca actcgacagc cgggtggaag
     9121 gatttatgct cggcgagctg gatatgctga aaaacggtat caactcgatg gcaatgtcgc
     9181 tggctgctta tcacgaagag atgcagcaca atatcgacca ggcgacgtcc gatctgcgtg
     9241 aaacgctgga gcagatggaa attcagaacg ttgagttgga tctggcgaaa aagcgcgccc
     9301 aggaagcggc gcgtattaaa tccgagtttc tggcaaatat gtcacacgag ctgcgtacac
     9361 cactgaatgg tgttattggc tttacccgcc tgacgctgaa aacagaatta acaccaacgc
     9421 agcgcgatca cctgaatacg attgaacgtt cggcaaataa tttgctggca attattaatg
     9481 atgttctcga cttctcgaaa ctggaagcag gtaagctgat tctggaaagt attccattcc
     9541 cactacgcag cacgctggat gaagtcgtta ctctgctggc acattcttct cacgataaag
     9601 ggttggaact gacgctcaat attaaaagcg acgtgcctga taacgtgatc ggcgacccac
     9661 tgcgattaca gcaaatcatc actaacctgg tggggaatgc aattaaattc accgagaatg
     9721 gcaacattga tattctggta gaaaaacgtg cgctgagtaa taccaaagtg cagattgaag
     9781 tgcagattcg ggataccggc attggtattc ctgaacgcga tcaatcgcgc ttattccagg
     9841 ccttccgaca ggctgatgcc agtatttccc gccgtcatgg tggcaccggg ctggggctgg
     9901 tgattacaca aaaactggtt aatgaaatgg gcggcgatat ttcgttccat agccagccga
     9961 atcgcggttc aactttctgg ttccacatta atctcgatct gaacccgaac attattatcg
    10021 aagggccatc catccagtgc ctcgcaggta agcgcctggc ctatgtcgaa ccaaactccg
    10081 cagcagcgca atgcacgctg gatattttaa gtgaaacgcc gctggaagtg gtttatagcc
    10141 caacgttctc cgcgctgcct cccgcgcatt acgacatgat gttgttaggc atcgcggtga
    10201 ccttccgcga gccgctaaca atgcaacatg agcgattagc gaaagcggta tcgatgaccg
    10261 atttcctgat gctggcactt ccttgccatg cacaagttaa tgctgaaaaa ctcaagcaag
    10321 atggtatcgg cgcgtgtctg ctgaaaccat taacacctac gcgcctgttg cctgccctga
    10381 cggaattttg tcatcacaaa caaaacacgc ttttgcctgt aaccgatgaa agtaagctgg
    10441 caatgacagt catggcggtt gatgacaacc ccgctaacct gaaacttatc ggcgcattgc
    10501 tggaagatat ggtgcaacat gtggaacttt gcgatagcgg gcatcaggca gttgaacggg
    10561 cgaaacagat gccgttcgat ttgatcttaa tggatattca aatgccggac atggatggca
    10621 ttcgggcctg cgaactcatc caccaactcc cgcatcagcg acaaacgccg gttatcgcgg
    10681 taacggcgca tgcaatggcc gggcaaaaag agaagctgct tggcgcaggg atgagcgatt
    10741 atctggcgaa accgattgaa gaagagcgat tgcataattt gttgttgcgc tacaagcctg
    10801 gcagcggtat ttcgtctcgc gtcgtgacgc ccgaagtcaa cgaaattgtg gtgaacccga
    10861 atgcgaccct cgactggcaa ctggcactac gccaggcagc aggaaaaacc gatttagcgc
    10921 gcgatatgct gcaaatgtta ctcgatttcc tgcctgaagt tcgcaacaaa gttgaggaac
    10981 agctggctgg agaaaacccg gaaggcctgg ttgatttgat tcataaactg catggcagtt
    11041 gcggctatag cggcgtacca cgtatgaaga atctctgcca gctgatcgaa caacagctac
    11101 gtagcggtac taaagaagaa gatttggagc cggagctgct ggaactgttg gacgagatgg
    11161 ataatgtcgc gcgcgaagcc agcaaaattc tcgggtaacg gacgtcttgt cgagtagcaa
    11221 tcggcttttt atgacgctgg tttcagattg gtgacaaagt gcctgtgttt atgccggatg
    11281 cggcgttaac gccttatccg gcctacaaaa ccaattaaat tcaatgaatt aaaaaatgat
    11341 gtagacctga tacatagcac aacaggctat tttgcgttta gcatcagtct caaaccggct
    11401 ccagatagag ccggttttgg ttttctgtct taacgcacca tgcacgggcg cttgttatcg
    11461 aacgtccagc caggaatcag atactgcatt cccatcgcat cgtcacgcgc gccaagcccg
    11521 tgtttctgat acagctcatg ggctttcatc acttgatcca tatcgatttc tacacccagc
    11581 cccggttttt ctggcacctg taccagcccg cctttgatct caaacggttc tttggtcagg
    11641 cgctgattac cttcctgcca gatccagtgc gtatcaatag cagtaatttt acccggtgca
    11701 gcggcggcaa catgggtaaa catcgccagg gaaatatcga agtggttgtt tgagtgtgaa
    11761 ccccaggtca ggccaaattc atggcacatt tgcgccacac gtaccgaacc ttgcatagtc
    11821 cagaaatgcg gatccgccag cgggatatca acggattgta acgagagcgt atggcccatc
    11881 tgccgccagt cggtggcgat catattggtt gcagtcggca ggccagtcgc gcgacggaac
    11941 tctgccatca cttcacgccc ggagaaacct tgctccgcac cacacggatc ttctgcataa
    12001 gccagcgaac ctttcaggta tttaccaatt ttaattgctt cgttcagcga ccaggcaccg
    12061 ttaggatcga gcgtaatacg cgcctgcggg aagcgttgcg ccagtgccac aatagactcg
    12121 gcttcttctt ccccggccag tacaccgcct ttcagtttga aatcgttgaa gccatatttt
    12181 tcatacgccg cttccgccag gcgcaccacc gcatccggcg tcatcgcttc ttcatgacgc
    12241 aggcgatacc agtcgcatga gtcatccggc tggctttgat acggcagagg tgtggctttg
    12301 cgattaccga cgaagaacag ataaccgagc atttcgactt cgctacgctg ttgaccatcg
    12361 cccagcagcg atgccacgtt tacccccaga tgctgcccca gcagatccag cattgccgct
    12421 tctatcccgg taactacatg aatagtggta cgcaaatcaa atgtctgcaa accgcgccca
    12481 ctagcatcac gatcggcaaa agtattacgc accagcgtca gaacgttttt gtattcaccc
    12541 agcgttttac ctaccaccag cggaatcgca tcttccagcg ttttacggat tttctcgccg
    12601 ccgggaattt cccctacgcc agtgtgaccg gaattatctt tgataatcac aatattacgc
    12661 gtgaagaacg gtgcgtgtgc accactcaga ttcatcagca tactgtcatg acccgccacc
    12721 gggatgacct gcatttcagt aacaacaggc gtcgtaaatt gagaactcat atttatgtcc
    12781 tttttcagaa tcaatgacgg ccgaaaacgg gacgtttacg gtcaaaggtc cagccgggga
    12841 tcaggtactg catcggacct gcgtcgttac gcgcaccgca aggcagacgt ttataggcct
    12901 catgtgcctt ttgtacctgt tcccagtcca gttccacgcc cagaccgggc gcatcaggaa
    12961 cggcaatttt tccgttttta atctccagcg gatttttggt caggcgacaa tcgccctcct
    13021 gccaaatcca gtgggtatcg atagcggtag gattacccgg tgccgccgcg cccacatggg
    13081 taaatatcgc cagagagata tcgaaatggt tgttggaatg gcagccccag gtcagccccc
    13141 agtcgtcgca aagctgcgcc acgcggactg caccggaaag cgtccagaag tgcggatcgg
    13201 caagtggaat atctaccgca ttgagcatca ccgcatgacc catttcgcgc cagttggtgg
    13261 cgatcatgtt agtcgcgacg ggcaagccgg tcgcccgtcg aaactccgcc atcacttcac
    13321 gtccggagaa gccctgttct gcgccgcatg gatcttcggc ataggtaaga acatcattca
    13381 gccctttgca taaagaaatg gcttcatcaa gcagccatgc accgttggga tcaacggtaa
    13441 tccgcgcatc cgggaagctt ttcttcaatg cacgaacagt gtcgatttct tgctcgccag
    13501 gtaacacccc gcccttaagt ttgaaatctt taaagccata gcgatcctgt gaggcttccg
    13561 ccagacgcac aacggcttcg ctgttcatcg ctttctgatg gcgcaactga taccactcat
    13621 ggttgcccgg cgtattttcc agataaggaa gatcggtttt ggtccgatca cctatataaa
    13681 acagataacc gaggacggta atagtctcgc gttgcttgcc tggccccaac agttcgcaga
    13741 ccggaacatt cagcgcctta cctagcaggt caagcaaggc ggcttccagc gccgccacgg
    13801 cattaacgcg caattcaaaa gtccaggcac ctttgccaaa ggtatcaaaa tcggctgcct
    13861 gattaccttt atgtacctgc tgaaccactt tattcaggcg cgcaacttcc tggcccagaa
    13921 ccatcggaat agcatcgacc agtgtttgat aaatcacctc tccgcccggc gcttcaccaa
    13981 tgccggtatg cccggcgtta tcggtgagta ccacgatatt gcgggtgaag tatgcgttat
    14041 gcgcaccacc aatattgagc aacatgctgt catgtccggc gaccggaatg actttcatat
    14101 cagtaataac aggactggat tgtgtcgcca tcttattgcc ccgcgacagg tttcagctcg
    14161 atacgcttga tatcgcccac cagcaccagg tagctcagta ccgcgattag ggcatgaaca
    14221 ccaacataaa tcagcgcccc attaaacgag ccagtcgtgc caacgatata accaattgcg
    14281 attggcgtga cgatgccaga aatgttaccg aacatgttga acaggccacc ggaaagaccg
    14341 ctgatctctt ttggcgcggt atctgccatt actgcccaac ccagcgcccc gatgccctta
    14401 ccgaagaagg ccagcgccat aaagccgatg atcatccact caacgttgac gtagttgcag
    14461 aacaccatca ccatcgacag caacatgccc attacgatcg gtgttttacg cgcaatgttc
    14521 agcgatcccg tgcggcgcat cagccagtcg gaaataattc cacccagcac accgccgata
    14581 aaaccgcaaa ccgccggaac ggaagccaca aagcccgctt tcagaatcga catcccacgc
    14641 gcctgcacca gataaaccgg aaaccaggta atgaaaaagt aagtcagggc gttgatacag
    14701 tactgaccga tataaacgcc gatcatcatc cgtgacccga gcagctgttt gatctgcccc
    14761 cacttcacgc tgaacggcac tttaactttg gtgctttgct gatccatatt gatcagcgcg
    14821 ccacccgcgg cgatatactc cagctctttc tgatttaccc ccggatgttg atttggctcg
    14881 tggatgactt tcaaccagat aaagctgatg acaatgccca gaccgcccat aaagaagaag
    14941 acgtgtgacc agcccacttc atgcgtcagc cagcccataa tcggcgcgaa gatcaccgtt
    15001 gcgaagtatt gagcggagtt aaaaatcgac accgccgttc ccctttcctg caccggaaac
    15061 caggccgcaa caatgcgact gttgccgggg aaagatggcg cttcagcaag cccgaccagg
    15121 aagcgcagag taaacagggc aacgataatg ccgaatccac taaagatatc gacgaagcct
    15181 tgcagcaagg taaacatcga ccagataaag atcgaccaga agtagacgcg ttttgaacca
    15241 aaacgatcca gcaaccaacc accagggatc tgcccgataa cataagccca tgagaaggca
    15301 gagaacacat agcccattcc cacgggatca aggccgatat ctttggccat ttccgaaccg
    15361 gcaatagaaa gcgtagcgcg gtcgccgtag ttgaaggatg tgacgataaa caacatcacc
    15421 actatccagt aacgagcatt cgtgcgtttt tccacactgc tcgcagcctg acttaaagaa
    15481 ctcattgttg cactcctgaa aattcgcgtt agccacgctc actctggact gcgacatcgc
    15541 caggaaatca gaggtgacgt agggtgtttt ttgccgtttt tataggtcgt tcgccgaata
    15601 cggcgcgtgt ttatatctgg caatagcagt ataaaaagcg cgccatagcg gctcaccgtg
    15661 caacaacaca acattaatgc gttcaatgag gcccgatttt ggcattagcc ctggacggtg
    15721 gaatccactt cacggaaatg aaaacaagaa caagaaagga agggtaaaaa cgaagaaata
    15781 aagaagagta tgaaatggat cgcttgactc taggcaaacg ccagtaaaaa tccgcgctat
    15841 gaagcagttt ttactggcat ttgcctgaaa agattcgatt cagcaccgct aaaacgacat
    15901 ttaccgctcg ctgacatatc aggacaacag cgtgccccac tgttcgaccc acggatttga
    15961 ttccgtttcc ggttccgggt tctcactggc gtcaatcaac agcatttcgc caacccgctg
    16021 agcgctctgt tcctgcaaca aggcatcgaa ctgtttgccg ccattgcaaa aatttacata
    16081 actgctgtcg ccgagcgcaa tcacgccata acgcagattc ggctggaagc ccagactatc
    16141 tttgattccc tgaaagagcg gcacaatgct atcaggaagg tcgccctgcc cggtcgtgga
    16201 cgtaaccacc agaacatact tatcctgata gggcagccag tcgcttaatt caggatcttc
    16261 aaataccgtt gctttgtggc cctgtgcggt cagaattgct tctgcttctt ccgccactaa
    16321 cagcgaattt ccgtacatgg tgccgacaaa aataccaatt tccgccatac cgttttctcc
    16381 ctggattagg aacttatctc tccatcctga cccgatggcg cactgaactc aaccctttca
    16441 ttttcaggaa gcagaccgcg ccagccaaat tgtgataacg cctgcatcca ggtgtcgtcc
    16501 aggcccgcgt gaatagtcag cggctcgcca gtaaaaggat gcgtcaatga caactgactg
    16561 gcatgtagca ttaaccgctg gaggccaaaa tgctcagcac cgctgcgatt ctggcgtaaa
    16621 tcgccatgtt tgctatcgcc aataatcgga tgacgcaaat gggcaagatg tcgtcgaagc
    16681 tgatgtttgc gtccggtttt cggctccagt tccaccaggc cgtagcgcgt ggtcgggtaa
    16741 cgtccggtcg ctaccggcat ttctacggtc gccagaccgc gataatgcgt cactgctggc
    16801 tgcgggcctt tatcttcgcg ggcaaattta tcagcgattt tgtccagttc ttccaccagt
    16861 ggataatcca acaccgcttc ttccatcaac cagccgcgca caatcgcatg gtaacgtttc
    16921 tggatttggt gctgttcaaa ctgttgtgcc agcagccgtc cggcctcgct ggataatccc
    16981 atcaacaaca caccagaagt gggtcggtcc agacgatgag cagtaaaaac atgctgtcct
    17041 atctggtcac gcacggtttg catgaccact actttctcgt cgcgatccag ccagctacgg
    17101 tgaaccagcc agccggaggg tttatttacc gcaaccagcc attcatcctg atagagtatt
    17161 tccagcatta gctcgcatca tccgcaaaaa gagcatccag tttttccaac tcagccagaa
    17221 taagcgcgcg ttgcggatgg tccgtcgcca gcgccatttc ataatagggt gcaacagcaa
    17281 aagcgcccgg taacggctgg ttgttattta acaaatcgtg cattcgcggg atcagccccc
    17341 actgcaacca ctccagtggt tccatggtgt ccataaagaa cggttgggta ctattaaatt
    17401 gatgcggctg gggttcatcg tttcgccagt gctgatgttc acgcagtaac gcttcaagcg
    17461 cctgcaactg gagacgaacg cggtcatgag tggtcataaa gcaacctcaa taagaaaaac
    17521 tgccgcgaag gatagcagga ataaaaaaag ggagcactgt attcacagtg ctcccggttc
    17581 gtttcgcagc attccagcta cttttgttgc tccctgctca tccttgacaa cttttcctct
    17641 ggccttgcgg ccaatcgttc atcctgaact attgcttcct gctcacacca ccccgatgtg
    17701 atacttcatc ctgaagtgtc cctggccttc ctgacccacc gaatcatcct gaccggttct
    17761 cattctccat cctggaggtg tcctttaacg cgtcctgcgt catcctcttc gcttcatcca
    17821 gaagcctttc cctgaaacac catcctggtg tgtcctgcag aagtgtcatc atcctgatgt
    17881 tcacttcgtt gtctgactcc ctgtcgacga agatagaatc gtctttttcg cgaagtctta
    17941 caaggcgctt acagacaatg ccttaaggaa aatttcatat gaaagttaag aattaacaat
    18001 caacattttg attttaaatg aaaataacga ttcttatctt tttaagtatt cgcttatttc
    18061 ctggcgatct cctacagagt ctgtaagaga tctctcacaa ccagatgggg tatttggatt
    18121 acagaagagg cttaagttga ttaaggaatt ccgcaagatt tgaggctaaa ttcgtccgtt
    18181 tacgtgttcc cagcgtctct ttgcacactt caccggagag attacacacc gagatgacct
    18241 ctaactcttc ttccagcgtc gcgataaata gcgtgggcgg cagttttagc cttttctgag
    18301 tcaccagatg accaatcaga ttttcctgca cccgacggaa gtcgtcttca ctccaggttt
    18361 gcagcaatgt cagtttgata tcgccaaact gcgcgtgcat atccccggca aactgagtcg
    18421 tataaaaggt atgaattgtg ggttgtatca caatatcaaa agcgcgttca accgcgttta
    18481 cattttgttc cccggtaaac ggctggggtt gccagtatac ggcatcttcg gtagttgaaa
    18541 taatgcacgg tgaaggaaca ccgtacaatt cctcacttaa cggccagctt ttatgctctt
    18601 catgccacgc atcgcagtag cgtgcagtaa aatctttcag ggcttgtgcg gtcaaatcgt
    18661 ccactaattt ctctcttcac ttaaaccaga tacacttgtc gtttagttta tctggtttat
    18721 gacggtgaaa catgtcttct tatgcaaacc atcaggcact tgcgggcctg actcttggaa
    18781 aatcaaccga ttaccgggat acctatgacg ccagcctact gcaaggcgtt ccacgcagcc
    18841 tgaatcgcga cccgctgggt ctgaaagcgg ataacctgcc ttttcacggt acggatatct
    18901 ggacgctgta tgaactttcc tggctgaatg cgaaaggttt gccgcaggtc gctgtcggtc
    18961 atgttgaact tgattacacc agcgtaaatc tgattgagtc gaagagtttt aagctctatc
    19021 tcaacagttt taaccagacg cgttttaata actgggatga ggtgcgccag acgctggagc
    19081 gcgacttaag cacttgcgct cagggtaaga ttagcgtggc gttatatcgt cttgatgaac
    19141 tggaaggcca gccgataggt catttcaatg gcacttgcat tgatgacaag gatatcacta
    19201 tcgataacta tgaattcact actgactatc tggagaatgc caccagcggt gaaaaagtag
    19261 tggaagagac gctcgtcagc cacctgctga aatcaaactg cctgatcacc catcaaccag
    19321 attggggttc gatccaaatt cagtatcgtg gacgccaaat tgacagagaa aaactgctgc
    19381 gttacctggt ctcattccgt catcacaacg agttccacga acagtgcgtg gaacgcatct
    19441 ttaatgacct gttacgcttc tgccagccag agaaattgag cgtttacgca cgttataccc
    19501 gtcgtggcgg tctggacatt aacccgtggc gcagtaatag cgattttgtc ccatcgacca
    19561 caagactggt tcggcaataa attttttctc aattttgcgt gctggattca cgcagaaggt
    19621 tgtgaaaggt catcaggcag ggctattgta atcaaaggga atgacgatat tcgtcccata
    19681 aggagttttt cttgattaca catattagcc cgcttggctc catggatatg ttgtcgcagc
    19741 tggaagtgga tatgcttaaa cgcaccgcca gcagcgacct ctatcaactg tttcgcaact
    19801 gttcacttgc cgtactgaac tccggtagtt tgaccgataa cagcaaagaa ttgctgtctc
    19861 gttttgaaaa tttcgatatt aacgtcctgc gccgtgaacg cggcgtaaag ctggaactga
    19921 ttaatccccc ggaagaggct tttgtcgatg ggcgaattat tcgcgctttg caggccaact
    19981 tgttcgcggt tctgcgagac attctcttcg tttacgggca aatccatcat accgttcgtt
    20041 ttcccaacct gaatctcgac aactccgtcc acatcactaa cctggtcttt tccatcttgc
    20101 gtaacgctcg cgcgctgcat gtgggtgaag cgccaaatat ggtggtctgc tggggcggtc
    20161 actcaattaa cgaaaacgag tatttgtatg cccgtcgcgt cgggaaccag ctgggcctgc
    20221 gtgagctgaa tatctgcacc ggctgtggtc caggagcgat ggaagcgccg atgaaaggtg
    20281 ctgcggtcgg acacgcgcag cagcgttaca aagacagtcg ttttattggt atgacagagc
    20341 cgtcgattat cgccgctgaa ccgcctaacc cactggtcaa cgaattgatc atcatgccgg
    20401 atatcgaaaa acgtctggaa gcgtttgtcc gtatcgctca cggcatcatt atcttccctg
    20461 gcggtgtggg tacggcagaa gagttgctgt atttgctggg aattttaatg aacccggcca
    20521 acaaagatca ggttttacca ttgatcctca ccggcccgaa agagagcgcc gactacttcc
    20581 gcgtactgga cgagtttgtc gtacatacgc tgggcgaaaa cgcgcgccgc cattaccgca
    20641 tcatcattga tgacgccgct gaagtcgctc gtcagatgaa aaaatcgatg ccgctggtga
    20701 aagaaaatcg ccgtgataca ggcgatgcct acagctttaa ctggtcaatg cgcattgcgc
    20761 cagatttgca aatgccgttt gagccgtctc acgagaatat ggctaatctg aagctttacc
    20821 cggatcaacc tgttgaagtg ctggctgccg acctgcgccg tgcgttctcc ggtattgtgg
    20881 cgggtaacgt aaaagaagtc ggtattcgcg ccattgaaga gtttggtcct tacaaaatca
    20941 acggcgataa agagattatg cgtcgtatgg acgacctgct acagggtttt gttgcccagc
    21001 atcgtatgaa gttgccaggc tcagcctaca tcccttgcta cgaaatctgc acgtaatctc
    21061 ccctcc
//
LOCUS       DAJTUI010000067        20769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTUI010000067 DAJTUI010000000
VERSION     DAJTUI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.067"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..579
                     /locus_tag="OO464_002685"
     CDS             <1..579
                     /locus_tag="OO464_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6227783.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="OO464_002686"
     CDS             597..1235
                     /locus_tag="OO464_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6227784.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="OO464_002687"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="OO464_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6227785.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO464_002688"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO464_002688"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR6227786.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="OO464_002689"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="OO464_002689"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6227787.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO464_002690"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO464_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6227788.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="OO464_002691"
     CDS             complement(5552..6613)
                     /locus_tag="OO464_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6227789.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="OO464_002692"
     CDS             complement(6610..8007)
                     /locus_tag="OO464_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6227790.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNASFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="OO464_002693"
     CDS             8163..9161
                     /locus_tag="OO464_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6227791.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO464_002694"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO464_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="OO464_002695"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="OO464_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO464_002696"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO464_002696"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR6227792.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO464_002697"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO464_002697"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR6227793.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..14855)
                     /locus_tag="OO464_002698"
     CDS             complement(14232..14855)
                     /locus_tag="OO464_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6227794.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14852..15274)
                     /locus_tag="OO464_002699"
     CDS             complement(14852..15274)
                     /locus_tag="OO464_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6227795.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15296..16071)
                     /locus_tag="OO464_002700"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="OO464_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16089..17141)
                     /locus_tag="OO464_002701"
     CDS             complement(16089..17141)
                     /locus_tag="OO464_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR6227796.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="OO464_002702"
     CDS             complement(17172..18014)
                     /locus_tag="OO464_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR6227797.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="OO464_002703"
     CDS             complement(18001..18882)
                     /locus_tag="OO464_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR6227798.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="OO464_002704"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="OO464_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20200..>20769)
                     /locus_tag="OO464_002705"
     CDS             complement(20200..>20769)
                     /locus_tag="OO464_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCR6227799.1"
                     /translation="KNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAILLS
                     FPGVPAIYIQSILGSRNDYAGAEKLGYNRAINRKKYHSEEITRELNDEATLRHAVYHE
                     LSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHVNIT
                     DLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5069 a         5664 c         5052 g         4984 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gacgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctagtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cggcacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attattttt
//
LOCUS       DAJTUI010000068        20741 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTUI010000068 DAJTUI010000000
VERSION     DAJTUI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.068"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..630
                     /locus_tag="OO464_002706"
     CDS             <1..630
                     /locus_tag="OO464_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HCR6227800.1"
                     /translation="NHKAVQRLMGTLSLKAAIKVKRYRSYRGEVGQTAPNVLQRDFKA
                     TRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKK
                     LNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSEC
                     FYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            732..884
                     /gene="hokA"
                     /locus_tag="OO464_002707"
     CDS             732..884
                     /gene="hokA"
                     /locus_tag="OO464_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6227801.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(1071..1283)
                     /gene="cspA"
                     /locus_tag="OO464_002708"
     CDS             complement(1071..1283)
                     /gene="cspA"
                     /locus_tag="OO464_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6227802.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1564..1854)
                     /locus_tag="OO464_002709"
                     /pseudo
     CDS             complement(1564..1854)
                     /locus_tag="OO464_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            2288..2998
                     /locus_tag="OO464_002710"
     CDS             2288..2998
                     /locus_tag="OO464_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6227803.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(3048..4022)
                     /gene="ghrB"
                     /locus_tag="OO464_002711"
     CDS             complement(3048..4022)
                     /gene="ghrB"
                     /locus_tag="OO464_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6227804.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(4126..4776)
                     /locus_tag="OO464_002712"
     CDS             complement(4126..4776)
                     /locus_tag="OO464_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR6227805.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4939..7137
                     /locus_tag="OO464_002713"
     CDS             4939..7137
                     /locus_tag="OO464_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6227806.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(7106..7546)
                     /locus_tag="OO464_002714"
     CDS             complement(7106..7546)
                     /locus_tag="OO464_002714"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6227807.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7543..8106)
                     /gene="tag"
                     /locus_tag="OO464_002715"
     CDS             complement(7543..8106)
                     /gene="tag"
                     /locus_tag="OO464_002715"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6227808.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            8264..8962
                     /locus_tag="OO464_002716"
     CDS             8264..8962
                     /locus_tag="OO464_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6227809.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            9191..10399
                     /locus_tag="OO464_002717"
     CDS             9191..10399
                     /locus_tag="OO464_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6227810.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10723..12414
                     /gene="eptB"
                     /locus_tag="OO464_002718"
     CDS             10723..12414
                     /gene="eptB"
                     /locus_tag="OO464_002718"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6227811.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12506..12582
                     /locus_tag="OO464_002719"
     tRNA            12506..12582
                     /locus_tag="OO464_002719"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12540..12542,aa:Pro,seq:cgg)
     gene            13326..14933
                     /gene="dppA"
                     /locus_tag="OO464_002720"
     CDS             13326..14933
                     /gene="dppA"
                     /locus_tag="OO464_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR6227812.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15241..16260
                     /gene="dppB"
                     /locus_tag="OO464_002721"
     CDS             15241..16260
                     /gene="dppB"
                     /locus_tag="OO464_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6227813.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16270..17172
                     /gene="dppC"
                     /locus_tag="OO464_002722"
     CDS             16270..17172
                     /gene="dppC"
                     /locus_tag="OO464_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6227814.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            17183..18166
                     /gene="dppD"
                     /locus_tag="OO464_002723"
     CDS             17183..18166
                     /gene="dppD"
                     /locus_tag="OO464_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6227815.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            18163..19167
                     /gene="dppF"
                     /locus_tag="OO464_002724"
     CDS             18163..19167
                     /gene="dppF"
                     /locus_tag="OO464_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6227816.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19197..20468)
                     /locus_tag="OO464_002725"
     CDS             complement(19197..20468)
                     /locus_tag="OO464_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6227817.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         5019 a         5100 c         5423 g         5199 t
ORIGIN      
        1 aaccataaag ctgttcagcg cctgatggga accctctcac ttaaagcagc gattaaggtc
       61 aagcgatacc gctcttacag aggagaggta gggcaaaccg cccctaatgt tctccaaaga
      121 gatttcaagg ctacgcggcc aaacgagaag tgggttaccg atgttactga atttgcagtc
      181 aatgggcgca agctgtattt gtctccagta atagatctct tcaacaacga agttatttct
      241 tacagccttt cggaaagacc agtgatgaac atggttgaga atatgctcga tcaggcattc
      301 aaaaagctta atcctcacga gcatcctgtt ctgcactctg accagggatg gcagtatcgt
      361 atgagaagat atcaaaatat ccttaaagaa catggtatta aacaaagcat gtccagaaaa
      421 ggcaattgtc tggataatgc tgtggtggag tgtttctttg gaaccttaaa gtcggagtgt
      481 ttttatcttg atgagttcag taatataagc gaactgaagg atgctgttac ggaatatatt
      541 gaatactata acagcagaag aattagcctg aaattaaaag gtctgactcc aattgaatat
      601 cggaatcaga cctatatgcc tcgtgtataa ctgtccaact ttttggggtc agtacaaagt
      661 gaggctaccc tatgcctgaa caccataagg ttagcctctt actcgttgga aatcaacaca
      721 gggggctggg aatgccgcag aaatatagat tactttcttt aatagtgatt tgtttcacgc
      781 ttttattttt cacctggatg ataagagatt cactgtgtga attgcatatt aaacaggaga
      841 gttatgagct ggcggcgttt ttagcctaca aattgaaaga gtaagagtct tcgacgggaa
      901 attattcccg ccttacttac ggcgttgcgc attctcattg cacccatatt tattcttcac
      961 aaaaataata atagatttta ttacgcgatc gattatttat ttcctgaaaa aaaataaaaa
     1021 aatccccgcc aaatggcagg gatcttagat tctgtgcttt taagcagaga ttacaggctg
     1081 gttacgttac cagctgccgg gcctttagcg ccgctttcga tggtgaagga cactttctga
     1141 ccttcgtcca gagatttgta accatcgttc tggatagcag agaagtgtac gaacacatct
     1201 ttagagccat cgtcaggagt gatgaagccg aagcctttgt cagcgttgaa ccattttacg
     1261 ataccagtca ttttaccgga catagtgtat tacctttaat aattaagtgt gcctttcggc
     1321 gatatggcgt gctttacaga ttttgaagcg ttaaaggaat gtgcactacg aggggtatca
     1381 acgataactc ttgaagggac ttgccttact acactgcttt aatggtctgt acgtcaaacc
     1441 gttgatgtgc attaagccac tcattggcgg gtgatgcaac aattattttt catatttatg
     1501 attaatcggt tggaaaacgg tcctgtcatc aggaccgtaa acagcaataa agtggataaa
     1561 agtctatttc atcaactgct tacttaatgc cgggttggct tgaatcaaac gcatcaattt
     1621 tagttcggca cttgaaggct tcacgcgtct ggattcccat tcctttacca tggcgactga
     1681 tacgcccaaa acccgggcga aatcatcgat ttttaatcct gtcccttttc ataactgctc
     1741 aatttcggta caggacgttg ttctgtgcgt cagggtaatt ttctgcgttt catctttaaa
     1801 aacaatctgt tccaggctgc tcaacagctc atgcattgga tctttatatt ccattgaaaa
     1861 ctcctcaaat cacactgcgg gatcgtgaat tgcatcgaag ctcattaaga atagtcggga
     1921 aaagcagaca cagcggggtg aagtcagggc atgctctggc tatgattcga ttcagcctgt
     1981 catttagcgt gacttatagc cacctaacga tctgataaat gccgatatat ctcttatcac
     2041 tcctgttttg taaagatttc gcaaatctaa ccttctgcga aggcgtaaat ctctggctac
     2101 aaaaaaggca acggcactat cattttttaa caatgaaaca acaaattaac cttgtttcat
     2161 tttctccatg attttaccag gaagattacg tagaaagggt atcctgcaag cctgtcctgg
     2221 actatccttg tcacgtcaga cacgcgtgtg tcgttgtgcg ctttttttgg gtgaaaggag
     2281 taagaaaatg gcgacaggaa agtcctgctc tcgctggttt gcgcctcttg cggcgttatt
     2341 aatggtagtt agcctgagtg ggtgttttga taaagaaggc gatcagcgta aagcgtttat
     2401 cgacttcctg caaaatacag tgatgcgtag cggtgaacgt ctaccaaccc tgactgccga
     2461 tcagaaaaaa cagtttggtc cttttgtctc tgattacgcg attctgtatg gttattctca
     2521 gcaggtgaat caggcgatgg attccggtct gcgcccggtc gtagacagcg ttaacgcaat
     2581 tcgcgtaccg caggattacg ttacgcaaag cggtccgctg cgtgaaatga atggttcgct
     2641 gggtgtgctg gcgcaacaac tgcaaaatgc gaagttgcag gccgatgctg cgcactctgc
     2701 gctaaaacag agcgatgacc tgaaaccggt atttgatcag gcattcacca aagtggtaac
     2761 gacgccagct gatgcattgc aaccgttgat tccggcggca caaactttca cgcaacaact
     2821 ggtgatggtt ggagactaca ttgctcagca aggtactcag gtgagctttg tcgcaaatgg
     2881 catccagttc ccgacttcac agcaggcaag tgaatataac aaactgattg cgccattacc
     2941 ggcacaacat caggcgttta atcaggcctg gactacagca gttactgcaa cccaataaag
     3001 agtaaaagcc cgagcgatta tcgggcttta cttcacgcag tcgcagctta gtccgcgacg
     3061 tgaggattca cacagttctt ctcaaccttt ccttgtaacg catcaatcaa attatccacg
     3121 gcacaggcgg ccatgccata acgcgtctca tgggtggcag atccaatatg cggtactgcg
     3181 acgacgttgg ccattgagag caacggcgaa tctacggaca gtggctcttg ttcgaagaca
     3241 tccagcccgg cggcgtgaat ttcgcctttc tgcaatgctg cgatcagtgc attttcgtca
     3301 accaccgggc cacgtccggc attaatgaaa atggcggagg atttcatttt ggcgaattgt
     3361 tctgcgccaa acagatgatg cgtctcatca gttaacggca ggatcaggca aacgaaatct
     3421 gactcttgta acagagtatc caaatcgcag tagcgggcgt tgaagcgttc ttctgcttct
     3481 ttatggtggc ggcgcgcgtt atagaggatg ggcatgttga agccaaagtg cacacgttgt
     3541 gccagcgcca tgccgatccg tcccatcccg acaatgccca gtgttttatg gtgaacgtca
     3601 gtgccgtacc agtccgggcc tatgctcgcg gtccattcgc ctgcttttac ccgttctgct
     3661 acctccacaa cccgacgagc ggtagacaac accagcgcca tcagcgtatc ggcgacggtt
     3721 tctgttaata cggttggcgt gtgcatcagc agaatttttc gggcggtaag cgcatcgaca
     3781 tcaaaattgt catagccgac ggagatcgtt gatgtggcac gcagtttcgg cattttttcc
     3841 agcaatgcgg catctacatt ctcgtttgaa cccagtaaac cttcagcttc tgcaaaaatt
     3901 gctgcatttt gaacgacggt ttgtgggctg aggtttgcca cctggtgaac ggtgaaatgc
     3961 gcttgcaggc gttgcagtaa atcatcaggt aaggctttgt agaggataac ggacggcttc
     4021 atgcttctct ccattcactg ataaggcgat caccttagcc tgaaatatga tgaaggaaaa
     4081 ctgaagaatg ccaggtgatt tctgcatcac ctggcatgaa agggattaca gcgggcttaa
     4141 ggtaatttct acacggcggt tttgcgcctt accttctgcg gtgctgttgc tggcgattgg
     4201 gttagccggg ccaaggccct gagtacggat gcggctggcg tccacgccct gggtgatcaa
     4261 cgcgctggca acggaatccg cacgctgctg ggagagacgc atgttcaggt cgtgaccccc
     4321 cgtgctgtcg gtataaccaa tcacgttaac cgccgttttc ggatactctt tcagcaccat
     4381 tgccacgccg gtcagggtgt tagcgcctgc cggtttcagg ggagcgctgc tgctgtcgaa
     4441 ggtcacattg ttcggcatat tgaggataat gttatccccg ctgcgggtta cgctaacacc
     4501 agtgccgcgc attttgtcac gcagcttcgc ttcctgcaca tccatgtaat aacccacgcc
     4561 gccgcccaga gctgcgcctg ctgctgcgcc aatcagcgcg cctttaccgc gatctttctt
     4621 cgaagaagag agcgcaccaa tacccgcgcc cacgagagag cccagacctg cgccgatagc
     4681 agatttgcct gcttcgcgtt cgccggtgta agggttagtt gtgcagccag ataccgccag
     4741 agcgccactc actacggcgg caagtaagat aaacacgttt cttcattgtt aatccttaat
     4801 aaccttttta ttctttgtca cgggttccgt ggcgggagat tatgccgcgt gaacatgaag
     4861 attattcctg ggaatactcg gaaatttgta agtaatattt aactgctcaa tacatctaac
     4921 ctttcaggag tcttcggttt ggccaactca tcctcacgat attccgttct gactgccgcc
     4981 cactgggggc ccatgctggt tgaaaccgac ggcgaaaccg tgtttagctc gcgtggcgcg
     5041 ttagccacag gaatggaaaa ctccttgcag agcgcggttc gcgaccaggt tcacagcaat
     5101 acgcgggtac gatttccaat ggtgcgcaaa ggctttctcg cgtcaccgga aaacccgcaa
     5161 ggcattcgtg ggcaggatga atttgttcgc gtgagttggg atgaggcgct ggcaggcggt
     5221 tataccgggc atctggggga ttattcgacc ggtgcggcac aggcgatcat gccgtatgtc
     5281 gtaggtggca gcgaagttta tcaacagcaa accagttggc cgctggtgct ggaacatagc
     5341 gatgtcgtgg tgttgtggag tgctaaccca ctcaatacgc tgaaaattgc gtggaatgca
     5401 tccgatgagc aggggctttc ttacttttcc gcactgcgtg acagtgggaa aaagctgatc
     5461 tgcattgatc caatgcgatc ggaaaccgtc gatttctttg gcgataaaat ggagtgggtg
     5521 gcaccgcaca tgggcaccga tgttgcgctg atgctgggga tcgcccatac gctggtggaa
     5581 aatggttggc acgacgaagc gtttctggcg cgttgcacca caggttatgc cgtcttcgcc
     5641 tcttatttgc tgggcgagag tgacggaata gcgaaaaacg ccgaatgggc ggcagagatt
     5701 tgtggtgttg gcgcagcgaa aatccgcgag ctggcggcta ttttccacca aaataccacc
     5761 atgctgatgg caggctgggg aatgcagcgc caacagtttg gtgagcaaaa acactggatg
     5821 atcgtcacgc tggcagcaat gttggggcaa atcggcacac ccggcggcgg ttttggtctt
     5881 tcttaccatt ttgccaatgg tggtgacccc acgcggcgtt ctgcggtgct ctcttccatg
     5941 cagggcagct tgccgggtgg ctgcgatgcg gtggataaaa tccctgttgc ccgcattgtt
     6001 gaagcactgg aaaaccctgg tggcgcatat caacacaacg gtatgaaccg acatttcccg
     6061 gatattcgtt ttatctggtg ggcgggcggt gccaacttta ctcatcatca ggataccaat
     6121 cgtctgatcc gtgcctggca aaaaccggag ctggtggtga tctctgaatg cttctggacg
     6181 gcggcggcga aacacgcgga tattgttctg cctgcgacta cctcttttga gcgtaatgat
     6241 ctcaccatga ccggtgatta cagtaatcag catctggtgc cgatgaagca agtggtgccg
     6301 ccacgctatg aagcgcgtaa tgattttgat gtttttgccg agttgagtga acgttgggag
     6361 aagggcggct atgcgcggtt tacggaagga aaaagtcagc tgcaatggct ggaaacgttt
     6421 tataacgttg cccggcagcg cggggcaagc cagcaggttg aattgccgcc atttgctgag
     6481 ttctggcaag ccaaccagtt aattgagatg ccggaaaacc cggacagcga gcggtttatt
     6541 cgcttcgctg atttttgccg cgatccgctg gcgcatccgt taaaaaccgc cagcggcaag
     6601 attgaaatct tctcacagcg tattgccgat tacggttacc cggattgccc tgggcatcca
     6661 atgtggctgg agccggacga atggcagggc aatgccgaac cagaacagtt gcaggtactt
     6721 tctgcccatc cggcgcaccg cctgcacagc cagctgaatt acagttctct gcgcgaattg
     6781 tacgcggtag caaaccgtga gcctgtcacc attcatcctg acgatgctca ggcgcgcggc
     6841 ataaccgaag gggatatggt gcgggtgtgg aactcccgtg ggcagatcct tgccggagcg
     6901 gtcattagcg agggaattaa acctggcgtg atttgcattc acgaaggggc atggccggat
     6961 ctggatttaa ccgctgacgg tatttgtaaa aacggcgcag tgaacgtgct gaccaaagat
     7021 ctccccagct cgcggctggg gaatggctgt gcgggtaata cgacgctggc atggctggaa
     7081 aaatacaacg gtccggaact gacacttaca gcgtttgaac caccggccag ctcataatcc
     7141 aggtgggtag ttgggtttca tcctgccatg cgcaatcgac aatgtgaaaa ccctgcgcac
     7201 ggtaaaaatc tatcgccggt tgattttttt gataaacctc cagcatcaga tggggatagc
     7261 gctgctgcac atactgcatc agcgccttac caataccgcg tctgacggcc ttcggtgcga
     7321 caaacattgc tgccagaaat cggccttcca taatgctgac aaaaccgaga agcttaccgt
     7381 cctcttccca gacccagttt tgcgcgttgg caagataggc atcccgcacc agcggaatgc
     7441 agtcacgcca gtaattcgct tttataaagg gatgccccca ggttgtactt tccagccaca
     7501 gttcgaggat cgcggggagt tctgaacgtt gcgcttcccg aatcatggtt tatttcccag
     7561 atagcaacag cagccaacca catgatcatt caccagccca catgcctgca taaaggagta
     7621 acagattgtg gtgccgacaa acttaaaacc acgttttttc agtgccttag atagggcgtc
     7681 ggaggcggac gtagatgtgg gaatttcgct caacgttgtg gcttgtgtca cctgtggctg
     7741 atgatttaca aacgaccaga caaagtcggg aaacggttcg ccgttctgtt ccatttgcag
     7801 gtacgcccgc gcattaccaa taattgcctg aattttccct cgatggcgga taatcccggc
     7861 gtcctgtacc agtctttcga catcctcttc ctgcattgct gcgaccttca ccggatcgaa
     7921 ctgatgaaag taggcgcgat agttttcgcg ttttttgagg acggtgatcc acgataaccc
     7981 agcctgctgc ccttcaaagc agatcatttc gaacagtttt ttactgtcag tttcaggcac
     8041 gccccactca ttatcatggt aggcaatata aagcgggccc tgactcaccc agccgcaacg
     8101 ttccatactt tccctcgcta tgtacccgat cgtaaaaatt tcagccgctt ttcttgacgc
     8161 atcggtgaaa aagtcaatat ttaatacagg gctactatgc ccgatatcct tcatacaatg
     8221 acaataatat tgccgaattc ggctcttccc tggagtcgtg ttaatgatca taaaaaaaag
     8281 cggtggtcgc tggcagctaa gcctgctggc gagcgtggta atcagtgcct tttttctcaa
     8341 cacagcttac gcctggcaac aagaatatat cgttgatacg caacccggac attccacaga
     8401 gcgttacacc tgggatagtg atcatcaacc tgattacaac gatattttgt cgcaacgtat
     8461 tcaaagtagc caaagggcgc tgggactgga agtcaatctg gcggaagaaa ctcctgtgga
     8521 tgtgaccagc agtatgagta tgggctggaa ttttccttta tatgaacagg ttacaaccgg
     8581 cccggtcgcg gcattacatt acgatggcac aaccacctcg atgtataacg agtttggcga
     8641 cagtactacc acgctgaccg atccgttatg gcatgccagc gtgagtagct taggctggcg
     8701 tgttgactcc cggcttggcg atctccgacc ctgggcgcaa atcagctata accagcaatt
     8761 tggcgagaat atctggaagg cgcaatcagg cctgagccgg atgacggcga caaaccagaa
     8821 cggcaactgg ctggatgtca ccgtaggcgc tgatatgttg ctcaatcaaa atattgccgc
     8881 ctatgctgcg ctaactcagg cagaaaatac cactaataat agcgactatc tgtatacgat
     8941 gggggttagc gccagatttt aacgtaacag tcacaattga aaccattgaa taacaacagt
     9001 tgtggcgata gtgggtgcta acttaccaaa taataaattt ggtgaataat tgtcgcgtca
     9061 ttcattcctg aactaaggca tttcattccg ttctgatggc atttcatgcc gtttttcccc
     9121 aagcataaaa tgcacttcgt tatggttgtc ggcagagatt tttccttttt attactgcag
     9181 gaatactgcc atgacacctt caaattatca gcgtacccgc tggctgacac tcatcggtac
     9241 tatcattacc cagtttgcgc tggggtcggt ttatacccgg agcctgttta atggcgcgct
     9301 ttctgccaag ctggatgcgc cggtaagcca ggtcgctttc tctttcggct tgttaagtct
     9361 ggggctggca atttcgtctt ctgttgcggg caaattgcag gaacgttttg gcgttaaacg
     9421 cgtcaccgtg gcttccggca ttttgctggg attaggcttc ttcctgaccg cgcattccaa
     9481 caacctgatg atgctgtggt taagcgccgg tgtgctggtc ggtctggcgg atggcgcggg
     9541 ttacctgctg acgctctcta actgcgtgaa gtggttcccg gagcgtaaag gtctgatctc
     9601 cgcgttcgct atcggttctt atggtctggg tagcctgggt ttcaaattta tcgacacgca
     9661 gctgctcgaa acggtgggtc tggaaaaaac ctttgtgatt tggggagcga ttgcgctggt
     9721 gatgattgtt ttcggcgcaa cgttaatgaa agacgcacca aaacaggaag tgaaaaccag
     9781 caatggtgtg gtggagaaag attacacgct ggcagagtcg atgcgtaaac cgcagtactg
     9841 gatgttagcg gtaatgttcc tgactgcctg catgagcggc ctgtacgtga ttggggtagc
     9901 gaaagatatc gcccaaagtc tggcacacct tgatgtggtt tccgcagcca atgcagtaac
     9961 ggttatttcc atcgctaacc tttcaggtcg tctggtgctg ggcattctgt ctgacaaaat
    10021 tgcccgtatc cgtgttatca ccattggtca ggtgatttcg ctggtgggta tggcggccct
    10081 gctgtttgca ccattaaatg cagtgacgtt ctttgcagcg attgcctgcg tggcatttaa
    10141 ctttggcggc actattaccg tctttccatc actggtcagt gagttcttcg gcctcaataa
    10201 cctggcgaaa aactacggtg tgatttacct cggtttcggt atcggtagca tttttggttc
    10261 gattatcgcc tcactgtttg gcggtttcta tgtgaccttc tacgtgattt tcgccctgct
    10321 gattctgtca ttggcgcttt ctacgacgat tcgtcagcca gagcagaaaa tgttgcgtga
    10381 ggcgcatggc tccctttaat gctcagccgc gagctactgg gcttcagctt ccgtgtgcat
    10441 caaacgcatc aacgaaatgt ataaacaatg actcgtaaaa tttgggccag ttaactggcc
    10501 ctttttactg ctttttgtaa cgctaaagtc tcttttcaaa cttgcatttt tgtaaatttg
    10561 tgcttcatgc acactctttc cccacacttt ttcgctttgc tgtggtctac ttattcgcgc
    10621 gtgtagattt tacttatctg actacctccg cacttttccc ctgccgggcc tgaaaagccg
    10681 ctaagcaggg tgttatcacc tgtttgtcca gggtttgttt gcatgagata catcaaatcg
    10741 attacacagc agaagctgag ctttttgctt gcaatctata ttggcctttt tatgaatggc
    10801 gcggtttttt atcgccgctt cggcagctat gcgcacgatt ttaccgtctg gaaaggcgtt
    10861 tctgctgttg ttgaactggc cgccaccgta ctggtgacct tctttttact acgtcttcta
    10921 tcgctgtttg gccgccgcag ctggcgtatt ctggcgtcgc tggtggtgct cttttccgca
    10981 ggtgccagct attacatgac cttccttaat gtggtcattg gttatggcat catcgcttcc
    11041 gtcatgacca ccgatatcga cctgtcaaaa gaagttgttg gtctgaactt tattctctgg
    11101 ttaatcgccg ttagtgcatt gcctcttatc cttatctgga ataaccgctg tcgctacacc
    11161 ttgctccgac aactgcgaac cccagggcag cgtattcgca gcctggtggt cgtcgtactg
    11221 gcgggtatta tggtttgggc accgattcgt ttgctggata tccagcagaa gaaagtggag
    11281 agggcgaccg gcgttgattt gccgagttat ggcggtgtcg tagcgaactc ttatctgcca
    11341 tcaaactggc tttctgcgtt ggggctgtat gcctgggcgc gggtcgatga atcttccgat
    11401 aataattcat tgcttaatcc ggcgaagaag tttacatacc aggctccaca aaacgttgat
    11461 gatacttatg tcgtgtttat catcggtgaa accacgcgtt gggaccatat gggtattttc
    11521 ggctatgagc gtaataccac gccgaaactg gcccaggaga aaaatctggc ggcgttccgt
    11581 ggttactcct gtgataccgc aaccaaactc tcactgcgtt gcatgtttgt acgtcagggg
    11641 ggcgcggaag ataatccgca gcgcacatta aaagaacaga acattttcgc ggttctgaag
    11701 cagttaggat tcagttctga cctctacgct atgcagagcg aaatgtggtt ctacagcaac
    11761 acgatggcag acaacattgc ttatcgtgag cagattggtg cggagccacg taatcgtggc
    11821 aagccggtag atgatatgtt gctggtagac gaaatgcagc aatcgctagg gcgcaacccg
    11881 gatggtaagc atctgatcat tctgcatacc aaaggttcgc attttaacta cacccagcgt
    11941 tatccgcgta gcttcgcgca gtggaagccg gaatgtattg gtgttgatag cggctgtacc
    12001 aaagcgcaga tgatcaactc ctatgacaac tcggtgacct atgtggatca ctttatctcc
    12061 agcgtgattg atcaggttcg cgataagaaa gcgattgtgt tctacgcagc tgaccacggt
    12121 gagtcaatta atgaacgcga gcacctgcac ggcacgccgc gtgaactggc accgccggag
    12181 cagttccgcg taccgatgat ggtctggatg tcagataaat atctggaaaa tccggccaat
    12241 gcgcaggcgt ttgcgcagct gaaaaaagaa gccgacatga aagtgccacg ccgtcacgta
    12301 gagctgtacg acaccatcat gggttgtctt ggctatactt caccggatgg tggaattaac
    12361 gaaaacaaca actggtgtca catcccgcag acaaaagtgg cagcggctaa ctaagcggcc
    12421 tgctgacttt ctcgccgatc aaaaggcatt ttgctattaa gggattgacg agggcgtatc
    12481 tgcgcagtaa gatgcgcccc gcattcggtg attggcgcag cctggtagcg cacttcgttc
    12541 gggacgaagg ggtcggaggt tcgaatcctc tatcaccgac caaattcgaa aagcctgctc
    12601 aacgagcagg tttttttgca tttgtagctc atgaggatga gaacctccgg gggcagggag
    12661 gctcgactcg acgtaggcct gataagacgc gccagcgtcg catcaggcaa cggcaccgca
    12721 cttattgtcg gatgcggcgc gaccgcctta ttcgaccaac acaccacaca ttttcctgcc
    12781 aactttgcgc cccatatctc actatccatt aacggatttg tgacagaaac cattgtcttt
    12841 ttttatattt gcttaaatct tttttgcgtt acttatggct ggcgctggca ttttccgctg
    12901 tgcgttttag cgttcatggt attaatcgct cagatatcca ccttttcctc agattttttt
    12961 cattaaatgc ataaaactta atcaccaaat gttgctaaat attaagctga ttgttctggt
    13021 tatgttggag tagcacaaat ttctctgctg caaatagctg tttgaagttt ttttaatctt
    13081 tgtttgtgga ttctcaccgt tggtgtaaat cccggttggc tgtattgacg ttttcacatt
    13141 ctgttgacag attgtaggtc acgaggggca ttttatggag gatccgcact gttacactga
    13201 tgttaattag tacggcatcc ccacctcata acgttgaccc gaccgggcaa aaaacaaaaa
    13261 aggtcaggca gcgacaaccc actgcaaagg gttaaaacaa caaacatcac aattggagca
    13321 gaataatgcg tatttccttg aaaaagtcag ggatgctgaa gcttggtctc agcctggtgg
    13381 ctatgaccgt cgcagcaagt gttcaggcta aaactctggt ttattgctca gaaggatctc
    13441 cggaagggtt taacccgcag ctgtttacct ccggcaccac ctatgacgcc tcttccgtcc
    13501 cgctttataa ccgcctggtt gaatttaaaa tcggcaccac cgaagtgatc ccgggcctcg
    13561 ctgaaaagtg ggaagtcagc gaagacggta aaacctatac cttccatctg cgtaaaggtg
    13621 tgaagtggca cgacaataaa gaattcaaac cgacgcgcga aatgaacgcc gatgacgtgg
    13681 tgttctcgtt cgatcgtcag aaaaacgcgc aaaacccgta ccataaagtt tctggcggca
    13741 gctacgaata cttcgaaggt atgggcttgc cggagctgat cagcgaagtg aaaaaggtgg
    13801 acgataacac cgttcagttt gtgctgactc gaccggaagc gccgttcctc gctgacctgg
    13861 caatggactt cgcctctatt ctgtcaaaag aatatgctga cgcgatgatg aaagccggta
    13921 caccggaaaa actggacctc aacccaatcg gaaccggtcc gttccagtta cagcagtacc
    13981 aaaaagattc ccgtatccgc tacaaagcgt ttgatggcta ctggggcacc aaaccgcaga
    14041 tcgatacgct ggtcttctct attacccctg atgcttccgt gcgttacgcg aaattgcaga
    14101 agaatgaatg ccaggtgatg ccgtacccga acccggcaga catagctcgc atgaagcagg
    14161 ataaatccat caacctgatg gaaatgccgg ggctgaacgt cggctacctc tcgtataacg
    14221 tgcagaaaaa accgctggat gacgtgaaag ttcgccaagc actaacctac gcggtgaaca
    14281 aagacgcgat catcaaagcg gtttatcagg gcgcgggtgt atcagcgaaa aacctgatcc
    14341 cgccaaccat gtggggctat aacgacgacg ttcaggatta cacctacgat cctgaaaaag
    14401 cgaaagcctt gctgaaagaa gcgggtctgg aaaaaggttt ctccatcgac ctgtgggcga
    14461 tgccggtaca acgtccgtat aacccgaacg ctcgccgcat ggcggagatg attcaggcag
    14521 actgggcgaa agtgggcgtg caggccaaaa tcgtcaccta cgaatggggt gagtacctca
    14581 agcgtgcgaa agatggcgaa catcagacgg taatgatggg ctggactggc gataacgggg
    14641 acccggataa cttcttcgcc accctgttca gctgcgccgc ctctgaacaa ggttctaact
    14701 actcaaaatg gtgctacaaa ccgtttgaag atctgattca accggcgcgt gctaccgacg
    14761 accataacaa acgcgttgaa ctgtacaagc aagcgcaggt ggtgatgcac gatcaggctc
    14821 cggcactgat catcgctcac tccaccgtgt ttgaaccggt acgcaaagaa gtcaaaggct
    14881 atgtggttga tccattaggc aaacatcact tcgaaaacgt ctctatcgaa taattaaaag
    14941 ccatacaaga ctgatggcaa aggcaaaact gcctgatgtg ctccgcttat caggcctacg
    15001 aaaattctgc aatgtattga atttgcacga ttttgtaggc cggataaggc gttaacgccg
    15061 catccggcat aaacaaagcg cactttgtca acaatctgta tacccggtgg cgctgtgcct
    15121 ctgcgtgcag cgccatccgg cagcaatact tcattctctg tccgacaagg gcgggaattg
    15181 atttgtgagc aatacagaca cgcagttcca ggctgcgagt cactatagag aatccgggtt
    15241 atgttgcagt ttattctccg acgtttggga ctcgtcatcc ccacgtttat cggtattacc
    15301 cttctcacat ttgcctttgt ccacatgatc ccgggcgatc cggtgatgat catggcaggc
    15361 gaacgtggga tctcccctga gcgtcacgtg cagctgctgg ctgaactcgg cttagataaa
    15421 ccgatgtggc agcagtatct ccattacatt tggggcgtaa tgcacggcga tctaggcatt
    15481 tcaatgaaaa gccgaattcc agtatgggaa gagttcgtgc cgcgctttca ggccacgctg
    15541 gaacttggcg tctgcgcgat gatttttgct acggcagtcg gtattccggt tggtgtgctg
    15601 gctgcggtta aacgcggttc cattttcgat cacactgcag ttggcctggc gctgaccggc
    15661 tactcgatgc cgatcttctg gtggggcatg atgctgatca tgctggtttc ggtgcactgg
    15721 aacctgacgc ccgtctccgg tcgcgtgagc gatatggtgt tcctcgatga ctccaatccg
    15781 ttaaccggtt ttatgctaat cgacaccgcc atctggggtg aagacggcaa ctttatcgat
    15841 gccgtcgccc atatgatctt gcccgccatt gtgctgggta ctattccgct agcggtcatt
    15901 gtgcgtatga cacgctcctc gatgctggaa gtgctgggcg aggattacat ccgcaccgcg
    15961 cgcgccaaag ggctaacccg catgcgggtg attatcgtcc atgcgctgcg taacgcgatg
    16021 ctgccggtag tgaccgttat cggcctccag gtgggaacat tgctggcggg ggcgattctg
    16081 accgaaacca tcttctcgtg gcccggtctg gggcgctggt tgattgacgc actgcaacgc
    16141 cgcgactatc cggtagtgca gggcggcgta ttgctggtgg cgacgatgat tatcctcgtc
    16201 aacttgctgg tcgatctgct gtacggcgtg gtgaacccgc gtattcgtca taagaagtaa
    16261 ggggacatca tgtcacaggt tactgaaaat aaagtgatta gcgcaccggt gccgatgacc
    16321 ccgttacagg agttctggca ctattttaaa cgcaacaaag gcgcggtcgt cgggctggtt
    16381 tacgtcgcca tcgtgctgtt catcgcgatc tttgccaact ggattgcgcc ttataacccg
    16441 gcggatcagt tccgcgatgc gctgctcgcc ccgccagcct ggcaggaagg cggcagtatg
    16501 gcacacttgc tgggcaccga tgacgtcggt cgtgatgtgc tgtcgcggct gatgtacggc
    16561 gcgcgtttat cgctgctggt tggctgtctg gtggtggtgt tgtcgctgat tatgggcgtt
    16621 attctcggcc tgatcgccgg ttacttcggc ggcctggttg ataacattat catgcgcgtg
    16681 gtcgatatca tgctggcgct gccaagtctg ctgttggcac tggtgctggt ggcgattttc
    16741 ggcccgtcga ttggtaacgc cgcgctggcg ctgaccttcg ttgccttgcc gcactatgtg
    16801 cgcttaaccc gcgccgccgt gctggtggag gtgaatcgcg attacgtcac cgcctctcgc
    16861 gtggcaggtg ccggggcgat gcgccagatg tttattaaca tcttcccgaa ctgccttgcg
    16921 ccgctgattg ttcaggcgtc gctcggtttc tctaacgcca ttctcgatat ggctgctctt
    16981 ggtttcctcg gcatgggggc acagccgcca acgcctgagt ggggcaccat gctctccgac
    17041 gtgttgcagt tcgcgcaaag cgcctggtgg gtcgtgacct tccctggtct ggcgatcctg
    17101 ttgacggtgc tggcatttaa cctgatgggt gacggtctgc gtgacgcgct cgatcccaaa
    17161 ctaaagcagt aagaggttcg agatggcgtt attaaatgta gataaattat cggtgcattt
    17221 cggcgacgaa agcgcaccgt tccgcgccgt agaccgcatc agctacagcg taaaacaggg
    17281 cgaagtggtc gggattgtgg gtgagtccgg ctccggtaag tcggtcagtt cactggcgat
    17341 tatggggctg attgattatc cgggccgcgt aatggcagaa aaactggagt ttaacggcca
    17401 ggatttgcag cgtatctcag aaaaagagcg ccgcaacctg gtgggtgccg aagtggcgat
    17461 gatcttccag gacccgatga ccagccttaa cccgtgctac accgtgggtt tccagattat
    17521 ggaagcgatt aaagtgcatc agggcggcaa caaaagtacc cgccgtcagc gggcgattga
    17581 cctgctgaat caggtcggta ttcccgatcc ggcttcgcgt ctggatgttt acccgcatca
    17641 gctttccggc ggcatgagcc agcgcgtgat gatcgccatg gcgattgcct gtcggccaaa
    17701 actgctgatt gccgatgaac cgaccaccgc gctggacgtg accattcagg cgcaaatcat
    17761 cgaactactg ctggagctac agcagaaaga gaacatggcg ctggtgttaa ttacccatga
    17821 cctggcgctg gtggcggaag ctgcgcataa aatcattgtg atgtatgcag gtcaggtagt
    17881 agaaacgggc gatgcacacg ccatcttcca tgcgccgcgt cacccgtata ctcaggcatt
    17941 gctgcgtgcg ctgccagaat ttgctcagga caaagaacgt ctggcgtcgt tgccaggtgt
    18001 tgttcccggc aagtatgacc gcccgaacgg ctgcctgctt aacccgcgct gcccctatgc
    18061 cactgacaga tgtcgcgctg aagaaccggc gctgaatatg ctcgctgatg ggcgtcagtc
    18121 caaatgccat tacccacttg atgatgccgg gaggcccaca ctatgagtac gcaagaggcc
    18181 acctcgcaac aaccgctgtt gcaggctatc gacctgaaaa aacattatcc ggtgaagaaa
    18241 ggcatgttcg tgccggaacg tctggtgaag gcgctggacg gcgtttcgtt taaccttgaa
    18301 cgtggcaaaa cgctggcagt agtgggcgaa tctggctgcg gtaaatcgac cctcggtcgg
    18361 ttgctgacga tgatcgaaac gcccaccggc ggcgagctgt attatcaggg gcaggatctg
    18421 ctcaagcacg atccgcaggc gcagaagctg cgtcggcaga aaatccagat cgtcttccag
    18481 aatccctacg gttcgctgaa tccgcgtaag aaagttgggc aaattctcga agagccgctg
    18541 cttattaaca ccagcttaag caaagaacag cgtcgggaaa aagccctgtc gatgatggca
    18601 aaagtcggcc tgaaaaccga gcactatgac cgctatccgc acatgttttc cggcggacaa
    18661 cgtcagcgta tcgctattgc ccgtggtctg atgctcgacc cggatgtggt gattgccgat
    18721 gagccggttt ctgcgctgga cgtctcagtg cgtgcgcagg tgctgaatct gatgatggat
    18781 ttgcagcagg agttggggct ttcttatgtc tttatctccc acgacctgtc ggttgtggag
    18841 cacattgctg atgaagtgat ggtgatgtac ctaggccgct gcgtggagaa aggaacgaag
    18901 gaccaaatct tcaataaccc gcgccatccg tacacccagg cgctgctctc cgcgacgcca
    18961 cgcctgaacc cggacgatcg tcgtgaacgc atcaaactca ccggtgaact gccaagcccg
    19021 ctgaatcctc cgccgggttg cgccttcaac gcccgctgtc gtcggcgctt cggcccctgc
    19081 acccagttgc agccgcagct aaaagactac ggaggtcaac tggtagcttg ttttgctgtt
    19141 gatcaggatg aaaatccgca gcgttaactc acaaaaccgg ggccatgcgc tccggtttac
    19201 tcaatcagct tcaacagtgg cgacaagcat agcaaaatgc cgtaaagcag aatcagccag
    19261 gctttaaatc cgcgaagttt ttccagttgt gcgactttat agatcaggaa aaacggaatg
    19321 aggcacgaca caatgccata taacgggctt cccaactgaa agaacaccag cactgataca
    19381 cgaaacgaaa cccaaatcgt caacgtaatg acgataaaag cgcagatcgc cagagtcagc
    19441 atgcgtgagt taattttctt ggtatcaata attcggctta acagattgag aataatgcct
    19501 ttaatagcct cgtggaaacc gagataaatg ccaaagaatg cggtcagtac ggcaaagata
    19561 ttcagcaccg tagaggtgat atgaatgata tgtccaggga tcacctgcgc ggccagtgcc
    19621 agtgccgaga tattttgttc aaaggcagaa acggcttctt cgtggctaat tgagaaggta
    19681 aacgaaaagg caaaaaacag gatcaccgcg atgagcgtga tataactaat tcggtgggta
    19741 cgcagcgcga gccgggttgc cagtacttta tctgcttccc gtttacgata ggcaatattc
    19801 attgggttaa gtacctgaat aaataccgca gaaaagaagc aaaatggaat ggtaagcagg
    19861 acatcgcgga aaaagacgga ggcttgcggg aaggcggtta tattggcgaa attccagtgc
    19921 gggatcatcg caaaaccgaa cacgacaata atccctactt tgaccaccac cattggccca
    19981 gaaatcttaa acagtaatcg ttcaccacca gacgcaatcg ctatcagtac ggcgaaaata
    20041 gcgactttat aaagtagaga ttgtgaaaga tcggcatcgg ttaaaccgaa ggttttcagg
    20101 tacgaggcgc tgtcgaaaac cacggagaga gagtagataa aaatcccgtg gataatcatc
    20161 aaaaagtaga taaccccgag gaaaattccc cagttcttcc ccaggtaatg actgataata
    20221 tcggtgtagt cattacagga atcgctttca gaaagggttt ttaaataaat gtcctgcact
    20281 acccaggtga caggataagc aatgatcgct gcggtaataa atacccaaat tcccttcaag
    20341 ccaatttgta ctggcatcag cacggttccg gcaccaatcg ccatgcctat gcataataaa
    20401 acccagccga agtcgtagcg tgtaaacggc aatttctgat tgtgtttcga tagtgtgttg
    20461 tgctgcataa agtcctgtcc agaaagaagt cggcctcgca aaaatgaata aacccagttt
    20521 ctcttgcgca acaggtggag aagtcgaata cgcagtatgt aaaaaatgag gcaacagatt
    20581 cactggggag gtcgcgagga ggcgcaattc tacaaaattg cgcatttcac taccagtctg
    20641 gagtcagccc ggatatgaac tgcaactatt gcaaataaag aaattaagct ggaaggcatc
    20701 gcgcaatgag gaagattgcc gggtgctgac agtaaaaagc a
//
LOCUS       DAJTUI010000069        20693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTUI010000069 DAJTUI010000000
VERSION     DAJTUI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.069"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            82..351
                     /locus_tag="OO464_002726"
     CDS             82..351
                     /locus_tag="OO464_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6227818.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            345..710
                     /locus_tag="OO464_002727"
     CDS             345..710
                     /locus_tag="OO464_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6227819.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(711..1499)
                     /gene="bioH"
                     /locus_tag="OO464_002728"
     CDS             complement(711..1499)
                     /gene="bioH"
                     /locus_tag="OO464_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6227820.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1537..2220
                     /gene="gntX"
                     /locus_tag="OO464_002729"
     CDS             1537..2220
                     /gene="gntX"
                     /locus_tag="OO464_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6227821.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2279..2854
                     /gene="nfuA"
                     /locus_tag="OO464_002730"
     CDS             2279..2854
                     /gene="nfuA"
                     /locus_tag="OO464_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6227822.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3215..4531
                     /gene="gntT"
                     /locus_tag="OO464_002731"
     CDS             3215..4531
                     /gene="gntT"
                     /locus_tag="OO464_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6227823.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="OO464_002732"
     CDS             complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="OO464_002732"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6227824.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6670..9063)
                     /gene="malP"
                     /locus_tag="OO464_002733"
     CDS             complement(6670..9063)
                     /gene="malP"
                     /locus_tag="OO464_002733"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6227825.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9675..12380
                     /gene="malT"
                     /locus_tag="OO464_002734"
                     /pseudo
     CDS             9675..12380
                     /gene="malT"
                     /locus_tag="OO464_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="OO464_002735"
     CDS             complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="OO464_002735"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR6227826.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="OO464_002736"
     CDS             complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="OO464_002736"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR6227827.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14850..16448
                     /gene="rtcR"
                     /locus_tag="OO464_002737"
     CDS             14850..16448
                     /gene="rtcR"
                     /locus_tag="OO464_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR6227828.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16430..17188)
                     /locus_tag="OO464_002738"
     CDS             complement(16430..17188)
                     /locus_tag="OO464_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6227829.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17205..18035)
                     /gene="glpG"
                     /locus_tag="OO464_002739"
     CDS             complement(17205..18035)
                     /gene="glpG"
                     /locus_tag="OO464_002739"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6227830.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18080..18406)
                     /gene="glpE"
                     /locus_tag="OO464_002740"
     CDS             complement(18080..18406)
                     /gene="glpE"
                     /locus_tag="OO464_002740"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6227831.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18596..20101
                     /gene="glpD"
                     /locus_tag="OO464_002741"
     CDS             18596..20101
                     /gene="glpD"
                     /locus_tag="OO464_002741"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6227832.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20370..20600)
                     /locus_tag="OO464_002742"
                     /pseudo
     CDS             complement(20370..20600)
                     /locus_tag="OO464_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4890 a         5694 c         5217 g         4892 t
ORIGIN      
        1 agtcagtaga tactctaccc tgcttaagtg ctactttgtg ctatcttgca tgtaacacaa
       61 agtagcactt agggaactga tatgcagaca gtgaaattac ggcaacaagg aggagccatg
      121 atcgttacta tcccacgaga tcttgccatc gatcttggct ggtcaccggg cacagagctt
      181 accgtggaga aaaaaggcga tagcgttaat ttgcgcgcca cagagcacaa accgcgtggg
      241 cgtcttaccg tcgcgcagtt gttgagccaa atcgatgaaa gcgaaattac ggagctaaat
      301 cagtccacag aaggatgggc tgaagggaaa aaaggtaatg aagcatggta aaggcacgga
      361 cgccacatcg tggtgagatc tggtatttta accctgatcc ggttgccggg catgaacttc
      421 aggggccaca ttattgcatt gtggtaacgg acaaaaaact caacaatgtt ttaaaagttg
      481 ctatgtgctg cccgatttca acaggggcaa atgcagcacg ttccacaggg gtgacggtga
      541 acgtcctccc ccgtgatacg caaaccggta acctgcatgg cgttgtactt tgtcaccagc
      601 taaaagccgt cgatcttatt gcccgtggcg ctaaatttca taccgttgcc gatgaaaaat
      661 tgattagtga agttatcagt aaactggtga atttaatcga cccacaataa ttagctctca
      721 cttaccacca acaccctctg cttcaacgcc accagcaggt gacaaaactc gaccggatgc
      781 gaaataaacg gcgcatgggc cgctttggcg aagatatatg attcgctgtg aggccaaagt
      841 ttatccagca tcggcaccac tttgcgcggc accagaccgt cgagatagcc atacaatcgc
      901 aaaaacggca tggacacgtt ttgcagcggc tgacggagat cgaccgtttt caggatttcc
      961 agcccgccat taagcacgtc aacctccggc atcggtaacg ccagaacggt tttcttcaac
     1021 gcccgcgcat cctggcgcgc cgtttcagtc cccatggttt gtaacgccag gaaccgctcc
     1081 actgtacgct gaaaatcatc actgagttgc tgctgaaatc ccgccagcac gtccggtttt
     1141 atccccggcc actcgtcacg agcactaaaa caaggtgacg acgccacggt gaccagcgcc
     1201 tgaacacgct cgggatgggt taacgcaatc tggcttgcca ccagcccgcc cagactccag
     1261 cctaaccaaa tggctttatc aggtgcctgt tgcagcacgg cttcggccat atcagcaagt
     1321 gacagcgcac caaatccccg gctacgcccg aagccgggca ggtcaacaag gtgcagcgta
     1381 aaatgcgagc taagttcctc gtcaatgcaa cgccacactt cggcattcag tccccatccg
     1441 tgcagcagca caagatgaac attcccctga cctttggtct gccaccagat gttattcatc
     1501 cgctattgtt ctcttttgac ttacaaggat gaacatatgc taacagtacc gggattatgc
     1561 tggctatgcc gaatgccact ggcgttaggt cattggggga tttgttcggt ctgttcacgc
     1621 gccacccgca cagataaaac gttatgccca caatgtggat tacccgccac acactcccat
     1681 cttccctgcg gtcgctgcct gcaaaaaccg ccgccctggc aaagactggt cacggttgcc
     1741 gactatgcgc cgccgttaag tccgcttatc caccagctta aattttcccg gcgcagtgaa
     1801 atcgccagcg ccctgtcacg tctgttacta ttggaagtct tacacgctcg tcgcaccacc
     1861 gggttgcaat tgccggatcg catcatcagc gttccgttat ggcagcggcg tcactggcgt
     1921 cggggattta atcagagcga tttgctgtgt cagccgttat cacgctggtt gcactgccaa
     1981 tgggatagcg aagccgtcac acgtacacgg gccactgcga cccagcattt tctcagtgcc
     2041 cggctgcgca agcgcaacct gaaaaatgcc tttcgtcttg aattgcccgt gcaaggtcgc
     2101 catatggtga ttgtggatga tgtcgttacc accggaagta ccgtcgcaga gattgcgcag
     2161 ttgcttttac gcaatggtgc ggcgactgtc caggtctggt gcctttgtcg aaccttgtag
     2221 agcctcgatg atgggcgtat tataaccaac taaaatagtc aactattagg ccattactat
     2281 gatccgtatt tccgatgctg cacaagcgca ctttgccaaa ctgctggcaa atcaggaaga
     2341 agggacacaa atccgcgtat ttgtgattaa ccctggcacg cctaacgctg aatgtggtgt
     2401 ttcttattgt ccgccggatg ccgtggaagc caccgacact gccctgaaat ttgacctgct
     2461 gaccgcgtat gttgatgagt taagcgcacc atacctggaa gatgcagaga tcgactttgt
     2521 taccgaccag ttgggttctc agttaacgct gaaagccccg aacgccaaaa tgcgtaaagt
     2581 ggcagacgat gcaccgctga tggagcgcgt ggagtatatg ctgcagtcgc agatcaaccc
     2641 acagcttgct ggtcacggtg gtcgcgtttc gctgatggaa atcaccgaag acggttacgc
     2701 cattctgcaa tttggcggcg gctgtaacgg ttgttccatg gtcgatgtga cgctgaaaga
     2761 agggatcgag aagcagctgc tgaacgaatt cccggagctg aaaggtgtgc gcgatctcac
     2821 cgaacaccag cgcggcgaac actcctacta ctaagttatc ccctcttttg tagattgccc
     2881 gatgcgacgc taaagcgtca tttcgggcct acgttgaaca tgtgccagac gttggcacag
     2941 cattgctttg gcaaatccct cccttcgttt ccccgcgata atatgaccaa cctctcataa
     3001 tttaaattta ccccgctctg gtgattctca aacgccaaat gttacccgta tcattcacat
     3061 gggtaccaaa catactcctg acatctgact acaataatta gttttagtgg gtatcagtcg
     3121 tggtgccgca atatctctgt tcccgattgg gataattaga gtttgtcgtc agaaaattga
     3181 cgttacccat aacaaatgaa aggccaggta aatcatgcca ttagtcattg ttgctatcgg
     3241 tgtaatcttg ttgttgctcc tgatgatccg cttcaaaatg aacggcttca tcgctctcgt
     3301 cctcgtggcg cttgctgttg gattaatgca aggaatgccg ctggataaag ttattggctc
     3361 catcaaagcc ggtgtcggcg ggacgctcgg tagccttgcc ctgatcatgg gttttggcgc
     3421 aatgctgggc aaaatgctgg cagactgcgg tggcgcacaa cgtatcgcca ccacgctgat
     3481 tgccaaattt ggtaaaaagc acatccagtg ggcggtggtg ttaaccggtt ttaccgttgg
     3541 ttttgccctg ttctatgaag tgggcttcgt gctgatgctg ccgctggtgt ttaccatcgc
     3601 ggcttccgcg aatattccat tgctgtatgt tggtgtacca atggcggctg cactgtctgt
     3661 gacccacggc ttcctgccac cgcatccggg cccgactgcg attgccacca ttttcaatgc
     3721 cgatatgggt aaaaccctgc tgtacggtac tattctggca atcccgaccg tgattctcgc
     3781 cggtccggtt tacgctcgcg tgttgaaagg tatcgataag ccaatcccgg aaggtctcta
     3841 cagcgcgaaa accttcagcg aagaagagat gccgagcttt ggcgtcagcg tctggacctc
     3901 tctggtgccg gttgtgctga tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg
     3961 tcacgctttc ctgccggtag cggagttcct cggtgacccg gtaatggcaa cgctgattgc
     4021 cgtgctgatt gcgatgttca cctttggtct gaaccgtggt cgttcaatgg atcagattaa
     4081 cgacacgctg gtttcttcca tcaaaatcat tgcgatgatg ctgttgatca tcggtggtgg
     4141 cggtgcgttc aagcaggtgc tggtagacag cggcgtggac aaatacattg cttccatgat
     4201 gcacgaaacc aacatttctc cgctgctgat ggcctggtcg attgctgccg tactgcgtat
     4261 cgcgctgggt tccgcaaccg ttgcggcaat cactgcgggt ggtatcgcgg caccgctgat
     4321 tgcaacgacg ggtgttagcc cagagctgat ggttattgcg gtgggttccg gtagtgtgat
     4381 tttctctcat gtgaacgatc cgggcttctg gctgttcaaa gagtacttta acctgactat
     4441 cggcgagacc atcaaatcct ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt
     4501 aggctgtctg ctgctgaata tggtgatttg ataacactga ctgccggatg catcgcatcc
     4561 gacatcaacc gcactctact tcttcttcgc tgcagctctg cgccgtctgt ccaaatcctt
     4621 cagcaacttg ttcacgccat catcggcaaa catcgactca agcgttgcgg aaagcttgcg
     4681 tcgccagttt ttatactggt aactggtgcc aggaatattc accggttcgg ccatatccag
     4741 ccagtcttcc ggctgtagtc ctaacagagc actgttactg tcggcaatgt agcgctgcaa
     4801 accacggttc agcgtcggcg tcatcgacat caacgatgcc ttatgcccgg cacgttttgg
     4861 cagacaacca tatttatgca gtgcatccag cagcccttgc ttcgccagtt cgcgatcctg
     4921 atacagaccg cgcagtacca cttcatccgg atacagcccc agggttttgc ccagcgttag
     4981 atccccgctc tcccagtaac cacgcagcgt tggcaggtca tgtgtcgccg caaccgccat
     5041 cgactgctcc ggatacgctt tcggtgcacg gaacgtcttc tcgtggtcgt tttcgaaata
     5101 gagcactttg taagagtaca caccgctgct gcgcagctta ccaacaatct ctaccggtac
     5161 ggtaccgaga tcttcaccaa tcaccataca gcgatgacgt ttactttcga gtgccagaat
     5221 cgagagcaga tcatccaccg gatagtgaac atacgcgccc tgatctgccg tctcgccata
     5281 cggtatccac cacaaacgca gcatcgacat cacatggtca attcgtaatg cgccgcagtt
     5341 ttgcatattg gcacgcaaca gctcgataaa cggttcatag gcacgcgcgg tgatgatatg
     5401 cgggtccatt ggcggtaatc cccagttctg ccccaacggg ccgaggatat ccggcggcgc
     5461 gccaaccgat gctttcaggc aatatagttc acggtcacac caggtttccg ccccaccttc
     5521 cgctacgcca accgccagat cacgatacaa gccaatcggc atttcatagc cctggcttat
     5581 ctcccagcag gcggcaaact ggctgtaagc cagccactgc aaccagagat aaaaatcgac
     5641 gtcatcacga tgttcttcgc agaactgacg cacttctggt gaatccacgt tcttatacat
     5701 ctctggccat gcaggccagc cccagcgcat ttcgtcctct ttcacttgct gggcatgtag
     5761 cgcatcaaag gcagcctgcc agaacaggct gtcgccctgc tctgcaacaa actggcgaaa
     5821 cgcggccatc tgctcatcat cacgttgcgc gaaacctttc cacgccattc gtaatgctgt
     5881 catttttagg gcggtaaccg tggagtaatc gacccagtcg gcatcgcgcg cctgttgcag
     5941 cgtctgttgc gtggtcggca actgccacca ggcctgagcc tcttcgctaa gatggaaatc
     6001 ttcaacggcg ttaacgtcga tataaatcac attcagccaa cggcgagaag acgggctgta
     6061 tgggctggcg ctctccggat ttgccggata gagcgcatga atcgggttca ggccaatgaa
     6121 cgacccgcca cgttttgcca catccaccag catcgctttg agatcgccaa aatccccaat
     6181 accccagttt ttttccgatc gcagcgtata aagctgaacg caggcacccc acagcttttg
     6241 tttattcagc aaagcctgcg gttcgtaaca acgtttcggg gcgacaatca cccggcaatg
     6301 cgcgcgctgg tcgtcctggg tgagtgttag cgtgtgataa ccttccggca gcttcgtcgg
     6361 tagattgaac gctttgcccc ccgttacatg gcctttgtac tgcgttcctt cttcggtggt
     6421 cagctgccag ctatattcgc cgctgccctc caccaccatc ggcatttttt tgccgctggt
     6481 ataaaccatg acattcggga ctggcgttac cgccactttc gtggcggtac gttgatgcat
     6541 cgcgtcaagc aaacgccgtt tggtttcggc gctaatcgac tgcggtttac cgtgggcatt
     6601 gatgtaattg gggctaatcc ccgccgccag cgcggcatta tccagacgtt tgctttccat
     6661 cgagcttcct tagcgttttg cctgccagat acgagcctga taatcgcgaa tagagcgatc
     6721 cgagctaaac ataccgcagc gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg
     6781 gtcgcggtac agcacatcca cctgcttttg tgcctcaaca taggctgcga aatccgccat
     6841 caccagatac ggatcgccgc cctgtttgcc gatactgtgc agcatctggt cgaaggcatg
     6901 cttatcgccg tcgctgtatt taccgctttc cagctctttc aatactgcgt ccagcacctt
     6961 atctttcttc cgccatttca ccgggtcgta gcctttggcc agaatagcct tcacttgttc
     7021 cacggtatga ccaaaaataa agatattttc ttcaccgact ttctcggcga tttcaacgtt
     7081 cgcaccatcc agcgtaccga cagtaagcgc accattgagc gccagtttca tattgccggt
     7141 accggaagct tctttacctg cagtcgaaat ttgttcggag atatccgccg ccgggatcag
     7201 tttttccgcc gccgaaacgc aataatccgg caggaacacc accttcaact tatcgccaac
     7261 cagtggatcg ttgttgatca cgtcagccac tttgttgatc gcaaagataa tattcttcgc
     7321 caggtagtag cccggtgccg ctttcgcgcc gaagaggaag acgcgcggta cgcgatcagc
     7381 ctgcaggttt tcacgaattt ctttgtacaa cgccagaata tgcagcagat tcaggtgctg
     7441 gcgtttgtac tcatgcagac gtttgatctg aatatcgaaa atcgcctgtg gattgatctc
     7501 aataccggta cgaactttca caaactccgc cagacagact ttattcgcct gcttgatctc
     7561 gcgatattgc tgacggaatt tcgcatcatc agcgaatttt tccagattga tcagctgatc
     7621 gagatcgtta gcccactctt tttgcagtga tttatccaac agagccgcca gtgcagggtt
     7681 gcactgtttg atccagcgac gtggggtaat accgttggta acgttatgga atttgttcgg
     7741 ccatagctgg tgatattccg ggaacagatc tttcaccacc agatccgagt gcagcgccgc
     7801 aacaccgttc accgcgaaac cgccaaccac acacaggttc gccatatgca cttgtttatc
     7861 gtgcaccacc gccagtttgg cccacacttt ttcatcgccc ggccaggttt tctccaccag
     7921 cgttttaaag cgagtattaa tttcgttaat aatctgcata tggcgcggca gtaagccttt
     7981 caccagtttc acatcccagc gttccagcgc ttctggcatc agggtatggt tggtgtaagc
     8041 gaaagttttg ctggtgatgg cccaggcgtc atcccagctc atctggtgct catcggtcag
     8101 cacgcgcagc agttctggaa tcgcgatagt tggatgggta tcgttcagct gaataacttc
     8161 gtaatccgcc agttcgtgca gtttacgccc cgccagatga tggcgacgca aaatatccgc
     8221 taccgaacag gcacactgga agtattgctg catcaggcgc agctttttac cggcagtatg
     8281 gttgtcgttt ggatagagaa ctttggtcag tttttccgca ttgatgcctt gctgttcggc
     8341 gcgcaggaaa tcaccgtcgt taaatttagt tagatcaaac ggatgcgcat gcgtcgcctg
     8401 ccacagacgc agcggctgcg ccacgccatt acgatagccg acaacgggga gatcccacgc
     8461 ttgaccggta atggtaaact ccggctccca gcgtccgtct ttcatcactt taccgccaat
     8521 ccctacctgc acatccagtg cttcgttgtg gcggaaccac gggtagttac tgcgatgcca
     8581 gtcatccggc gcttcaacct gtttgccatc gacaaaagac tggcggaaca aaccatattg
     8641 atagttcaga ccgtaacccg tcgcagactg accgacagtt gccattgagt cgaggaagca
     8701 cgccgccaga cgtcccagac caccgttacc cagcgccggg tcgatctctt cttccagcag
     8761 gtcagtcagg ttgatgtcat aagccttcaa cgaatcctgt acatcctgat accagccgag
     8821 attcaacagg ttgttgcccg tcagacgacc aatcaaaaac tccattgaga tgtagttaac
     8881 atgtcgctga ttcgccaccg gtttggcgaa tggctgagca cgcagcattt cggccagtgc
     8941 ttcactcact gccagccacc actggcgagg agtcatttca gccgcagaat ttaagccata
     9001 acgctgccac tgacgtgaaa gcacttcctg aaattgctta tcgttaaaaa taggttgtga
     9061 cataggagtt ccacttttct tagattttca acacaacgtt atcgctagtt tgccaggctc
     9121 gatgttgacc ttcctcatcc tgcgggggat taggcaggga ggagttgcgg ggatgagcaa
     9181 ggaaatgtga tctcgaccac ttaaagctag tgcaaaccac aggattagca gcaaatcaat
     9241 gccatacagc gcagaaaatc tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa
     9301 aagcggaagg taactctata aattaagtaa aggagtgaaa cagtttcaca agtaaaatat
     9361 ccagtgtgct ccatctcatt cttaatagat ttattaagat catcttttta gatggcactt
     9421 tcatcaggaa tgaagaagaa acccttgctt aaatgaatct gatgaacata agggaaacca
     9481 gtattcacgc tggatcagcg tcgttttagg tgagttgtta ataaagattt ggaattgtga
     9541 cacagtgcaa attcagacac ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc
     9601 cgaccttata accattaatt acgaagcgca aaaaaaataa tatttcctca ttttccacag
     9661 tgaagtgatt aactatgctg attccgtcaa aactaagtcg tccggttcga ctcgaccata
     9721 ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc gaacaacttc cggctggcgc
     9781 tgatcacgag tcctgcgggc tacggaaaga ccaccctcat ttcccagtgg gcggcaggca
     9841 aaaacgatat cggctggtac tcgctggatg aaggtgataa ccagcaagag cgtttcgcca
     9901 gctatctcat tgccgccgtg cagcaggcaa ccaacggtca ctgtgcgata tgtgagacga
     9961 tggagcaaaa acggcaatat gccagcctga cgtcactctt cgcccagctt ttcattgagc
    10021 tggcggaatg gcatagccca ctttatctgg tcatcgatga ctatcatctg atcactaatc
    10081 cagtgatcca cgagtcaatg cgcttcttta ttcgccatca accagaaaat ctcaccctgg
    10141 tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa tctgcgtgtt cgtgaccaac
    10201 tgctggaaat tggcagtcag caactggcat ttacccatca ggaagcgaag cagttttttg
    10261 attgccgtct gtcatcgccg attgaagccg cagaaagcag tcggatttgc gatgacgttt
    10321 ccggttgggc gacggcacta tagctaatcg ccctctccgc ccggcagaat acccactcag
    10381 cccataagtc ggcacgccgc ctggcgggaa tcaatgccag ccatctttcg gattatctgg
    10441 tcgatgaggt tttggataac gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca
    10501 ttttgcgctc aatgaacgat gccctcatca cccgtgtgac cggcgaagaa aacgggcaaa
    10561 tgcgtctcga agagattgag cgtcaggggc tgtttttaca gcggatggat gataccggcg
    10621 agtggttctg ctatcacccg ctgtttggca acttcctgcg ccagcgctgc cagtgggaac
    10681 tggcggcgga gctgccggaa atccaccgtg ccgccgcaga aagctggatg gcccagggat
    10741 ttcccagcga agcgattcat catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata
    10801 ttctgcttaa tcacgcctgg agtttgttca accatagcga actgtcgctg ctggaagagt
    10861 cgcttaaggc cctgccgtgg gacagtttgc tggaaaatcc gcagttagtg ttattgcagg
    10921 cgtggctaat gcaaagccaa catcgctatg gcgaagttaa caccctgcta gcccgtgctg
    10981 aacatgaaat caaggacatc agagaaggca ccatgcatgc agaatttaac gctctgcgcg
    11041 cccaggtggc gattaacgat ggtaatcagg atgaagcgga acggctggca aaactggcac
    11101 tggaagagct gccgccgggc tggttctata gccgcattgt ggcaacctcg gtgctgggtg
    11161 aagtgctgca ctgcaaaggc gaattgaccc gctcactggc gctagtgcag caaaccgaac
    11221 agatggcacg ccagcacgac gtctggcact acgccttatg gagtttaatt cagcaaagtg
    11281 aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga aacgcaggaa aaagcgttcc
    11341 agctgatcaa cgagcagcat ctggaacagc tgccaatgca tgagtttctg gtgcgcatcc
    11401 gagcacaact gttatgggcc tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg
    11461 ggattgaagt cttgtcgtct tatcagccac agcaacagct tcagtgcctg gcaatgttga
    11521 ttcaatgctc gctggcccgt ggtgatttag ataacgcccg tagccagttg aaccgtctgg
    11581 aaaacctgct ggggaatggc aaatatcaca gcgactggat ctctaacgcc aacaaagtcc
    11641 gggtgattta ctggcaaatg accggcgata aagccgccgc tgccaactgg ttgcgtcata
    11701 cggctaaacc ggagtttgcg aacaaccact tcctgcaagg tcaatggcgc aacattgccc
    11761 gcgcacaaat cttgctgggc gagtttgaac ctgcggaaat tgttctcgaa gaactcaatg
    11821 aaaatgcccg cagtctgcgg ttgatgagcg atctcaaccg taacctgttg ctgcttaatc
    11881 aactgtactg gcaggccgga cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa
    11941 aactggcgaa tcgcaccgga tttatcagtc attttgtcat cgaaggcgaa gcgatggcgc
    12001 aacaactgcg ccagctgatt caacttaata cgctgccgga actggaacag catcgcgcgc
    12061 agcgtattct gcgagaaatc aatcaacatc atcggcataa attcgcccat ttcgatgaga
    12121 atttcgttga acgtctgcta aatcatccgg aagtgccaga actaatccgc accagcccgt
    12181 tgacccaacg tgaatggcag gtcctggggc tgatctactc cggttacagc aatgaacaaa
    12241 ttgcgggcga actggaagtg gcggcaacca ccatcaagac gcatatccgc aatctgtacc
    12301 aaaaactcgg cgtcgcccac cgtcaggctg ccgtgcagca cgcgcagaaa ctgctgaaga
    12361 tgatgggtta tggggtgtga gtttagccgg ataatgcatt ggatccggtt tacatcttta
    12421 catcattcaa tactcacccg cgttacgcca tctgtttcta tcaaaccaaa ccgcaccggc
    12481 aagaaacgct ccaccaccgc gatattggtc agcagatggc atgaaggatg ggcgaccgta
    12541 aattctcccg cgcccgccag cgccatcggc agtaccagtt ggtcggcgag atattccccc
    12601 accgccgctg ggcttgccag gtagcgtttc acctctttca ctaactgcgc ggctaccacc
    12661 tcggcactga cgcgcttttc accgacgacg aaaaagcgtt cagtgatatt ttcactttcg
    12721 acttccagcg agacggtatt acccggcccc tggtcgcgcg gcaggttatg aatattctgt
    12781 tcatgcaggg aaaaaatccc cgccagtgta gcgatttcac gctcagcaac atggcgcggc
    12841 acaccagcta atagaacttc tccacgcatc tgcacaatgt tcccgcgctc gccaagttgc
    12901 aaggtgctaa acgatgccac gggcgagact tccgttgcca ccacaccgcc tccggcagga
    12961 taaaaaccgt gacgtaacag cgtggtttgc tgatgaattc ctattttcgc cagcagcggc
    13021 tccagcaccc ggcggataaa atccgcaggc ggcgccgacg ggttatcggt gccgccgctc
    13081 acttcaacac gcgaaggtcc atcggcaaac cacagcgcgg gcagcaccgt ttgcagcacc
    13141 agcgtacaac ttccggcgct accgatagca aagcggtaat cgccgccgcg cacggtgcca
    13201 ggccggaaga ccagacgctg cgatcccagc tccgcgcctt ccaccgttgc cctacaaatt
    13261 tccgcagccg cttttaccgc ggtcagatgc tggcgcagca gccccggttt cgcccgtccg
    13321 gctcgaatgc cagtgatggt aaatggctgg ccggttatca tcgacaggct cagcgccgag
    13381 cgcaggatct gcccgccgcc ttcgccctgt gcgccatcca gcgcaatcat ccttttcatc
    13441 atttatcctt ttacgcacac cacctgacgc agggtgtaga taacttccac cagatcgctt
    13501 tgtgccgcca tcaccgcatc aatatcttta tacgccatcg ggatttcgtc gatcacttcg
    13561 gcatctttac ggcattccac atgcgcggtg gcacgaattt ggtcttccac gctgaacagt
    13621 tttttcgctt tagttcggct cattacccgc ccggcaccgt ggctgcacga acagaacgac
    13681 tcttcatttc ccagcccacg gacgataaag ctttttgctc ccatcgaacc gggaataatt
    13741 ccatattgac cagcacgcgc agacaccgca cctttacgcg tcacgtagat ctcttcacca
    13801 aagagctgtt ctttttgcac atagttgtgg tgacagttga tctcttccat cgccagcgtt
    13861 tgtggctgtc tgaccgtttt ctgcgtaatg ctctgcaacg ccgttaccac attttccatc
    13921 atcgcatcgc ggttaaggct ggcgaaaagc tgcgcccagg ccacggctct taggtaatca
    13981 tcaaagtatt ccgtaccttc cataaagtac gccagatcac gcgacggcaa cgtctcaagt
    14041 tggtcctgca tctctttttg cgccagatcg ataaagtaag tcccgatggc gttaccaatt
    14101 ccgcgtgaac cggagtgcag cataatccac acctgctccg actcatcaag gcagatttca
    14161 ataaagtggt tacccgttcc cagcgttccc aggtgtttat agttgttagt gttaaggaaa
    14221 cgcggatatt tttgcgttaa ccactgataa ccggcctcca gctcagccca tttcgcatcg
    14281 acgttaaaag gcgggttttc ccatgcgcct ttatcacgtt tacaacgccc ggtagtacgc
    14341 ccgtgtggta cggccgtttc aatcgcttga cgcagctccg ccaggttttc aggcaggtct
    14401 gccgccgtta acgcggtacg cagcgcgttc attccacagc caatatccac gcccaccgcc
    14461 gccggaataa tcgccccttt ggtcgggatc acgctaccaa tggtggaacc tttacccagg
    14521 tgtacatcag gcattaccgc aatatgtttg aaaataaacg gcatcttcgc cgtattaata
    14581 agttgctgac gcgcatcggc ttctaccggc acgcctttgg tccacatttt taccggggca
    14641 ttttcagtgg tcagtaattc gtaattcatt ttgtttttct cttttcgttg tttgcggtcc
    14701 tgataattgc aaccgtcgtg ccagaaaatt aaataagcag attaatttat tgtttctaaa
    14761 aagattatat ctttatatcc gtaaacggag atttcccgca aagccgattt accgataatg
    14821 aaatatcgcc ttttataagg atatctaaga tgcgtaaaac agtggctttt ggctttgtcg
    14881 gtaccgtgct ggattatgcc ggacgcggca gtcagcgctg gtcaaaatgg cgtccgtcac
    14941 tctgtatata ccagcaagaa tcgttggtca tcgatcgact ggaattattg cacgatgccc
    15001 gctcgcgctc gctatttgaa acgcttaaac gcgatatcgc cagcgtttcg ccagaaacag
    15061 aagtggtggg cgttgagatt gaactgcata acccgtggga tttcgaagag gtttacgcct
    15121 gcctgcatga tttcgcccgt ggttacgagt ttcagccaga aaaagaagac tatttaattc
    15181 acatcaccac cggcacccac gtcgcgcaga tttgctggtt tctgctggcg gaagcgcgtt
    15241 atctacccgc ccggttgata caatcttcaa ctccgcgcaa aaaagaacag ccgcgcggcc
    15301 caggtgaagt gacgattatc gatctcgatt taagccgtta taacgccatc gccagccgct
    15361 ttgccgagga acggcagcaa acgctcgatt tcctcaaatc cggtattgcc acgcgtaacc
    15421 cccacttcaa ccgcatgatt gagcagatcg aaaaagtggc gattaaatcc cgcgcgccga
    15481 ttctgcttaa cggcccgacc ggcgcaggta agtcatttct ggcgcgacgc atcttcgagt
    15541 taaaacaggc acggcatcag tttagcggcg cgtttgtgga agtgaactgc gccaccctgc
    15601 gcggcgatac cgcgatgtcg acgctgtttg gtcatgtgaa aggcgcgttt accggggcgc
    15661 gggaatctcg tgaaggatta ttacgcagcg ccaacggcgg aatgttgttt cttgatgaga
    15721 ttggcgaact gggcgcagac gaacaggcaa tgctgctgaa agctattgaa gagaaaacct
    15781 tttacccgtt tggcagcgat cgccaggtga gcagtgattt tcagcttatc gccggaacgg
    15841 tgcgcgattt gcgccagctg gttgccgaag gcaaattccg cgaagatctg tacgcgcgga
    15901 tcaatctctg gaccttcacc ctgccgggtc tacgccagcg ccaggaagat attgaaccga
    15961 acctggatta tgaagtggag cgccacgcca cactcactgg tgacagcgtg cgttttaaca
    16021 ccgaagcgcg gcgtgcctgg ctggcctttg caacatctcc ccaggcaaca tggcgcggta
    16081 actttcgcga actttctgcc agcgtcacgc ggatggccac ctttgccact agcggacgca
    16141 tcactctgga cgtggtggaa gatgagataa accgtctgcg ctataactgg caggagagcc
    16201 gcccctccgc gcttacggcg ttgctgggcg cagaggcaga aaacatcgat ctcttcgacc
    16261 gtatgcaact ggaacacgtt atcgctatct gccgccaggc aaagtcgctt tctgctgccg
    16321 gacgacagct ttttgatgtt tcgcgccagg gcaaagccag cgtcaatgac gcggatcggc
    16381 tacgcaaata cctggcgcgt tttggtctga cgtgggaagc cgtgcaggat cagcacagct
    16441 ccagttgaat atggtggtcc gtcagcacct gcatcacgct tactggcggc ggggcgtcgg
    16501 tgtagacggc atcaaccatg ctgatgctcc ccatattgac catcgcatta cggccaaatt
    16561 ttgaatggtc aaccaccagc ataacgtggc gcgagttctc aataatggcg cgcttggtac
    16621 gaacttcgtg gtaatcgaac tccagcagcg agccgtcgct gtcgatgccg ctgatcccca
    16681 gaatgccgaa atcaaggcgg aactgggaga taaaatcgag cgtcgcttcg ccaatgatcc
    16741 cgccatcgcg gctacgtaat tcgccaccgg cgagaataat gcggaaatct tcttttacca
    16801 tcaacgtgtt agcaacgttg agattgttgg tgacaatgcg caaattgctg tgattgagca
    16861 gcgcgtgcgc caccgcttcc ggcgtggtgc cgatatcgat aaacagcgtc gagccattgg
    16921 ggatttgctc cgccactttg cgggcgatgc gctctttttc ttcggtctgg gtggctttac
    16981 gatcgtgcca cggcgtgtta accgaactgg aaggcagcgc cgcgccgcca tgatggcgca
    17041 ggatcaggtt ttgctctgcc agctcgttga ggtcgcggcg aatagtctgc gggctgacgg
    17101 agaagtgctc caccagttcc tcggtactga cataaccctg ctgtttaacc agttcgataa
    17161 taccgttgtg acgttgtgtt tgtttcattt ataaatccct ggaattattt tcgttttcgc
    17221 gcattgagcg aatcaacaaa agccatcgct aaacccacgg ctaatccggc gatgtgtgct
    17281 ccgttcgcca tcgacatccc aaacaaatca aaccatccgg cgacaatcca gatcagcgca
    17341 aagataatta acccacgttg caggtaaatg ccactttgcg gatcgcgttc gccacgtagc
    17401 cagacgtagc ccatcagcgc atacaccacg ccagaaagcc cgccaaacca cggcccgctg
    17461 aatttttgct gcatatagcc gcttaacagg gcgctgataa gcgtaatgac aattagctta
    17521 ccgctaccga ggcgtttttc caccgcgccg ccgagatacc accaccaaag caggttaaag
    17581 aggatatgca tcagcgagaa gtgcattaac gcgtgggtga agtaacgcca gaactcaaat
    17641 ttcagtgttg gatcgaatgg ccaggccagc cataacatca cttcctgatc gccgagaatt
    17701 tgcatggcaa taaacaccac cacgcaggcg atcatcatca cccaggtcac cggacctgcg
    17761 cgttcacgca aggcagcaaa gaaaggataa cggcgataat gcaggccact gccggtatgg
    17821 cctgccagcc agctggccgc cagataacgc ggatctgccg ggttttcgag aaaacgcgcc
    17881 agctccgccc gcacgcgctc ggcctgggac tcatccgcca gccagacatc gctttggtta
    17941 tgttgttgaa tcgtgaggat aacaccctgc gtcgccatgt aatcaacaaa cgcctgcgcc
    18001 acgcgggggt tagcaaaaga ggtaatcatc aacatcgttg ctgtcgctta ttccacacaa
    18061 aaggggacag tataaagcgt tacgcgccgt acgccacctc tgcgggaaac tgtcgctgcc
    18121 aggcttcaaa gccgccgtca atgctataga ccacatcgta gccctgttgc agcagatact
    18181 gcgccgcccc tttgctgcta ttgccgtgat aacacatcac catcaccgga gtgtcaaagt
    18241 cgttatcacg cataaacgcg cccagcgtgt cgttggttaa atggaaagcc tgcaccgcat
    18301 gccccatagc gaaactctgt ggatcgcgaa tatcgaccag caccgcctct ttttcctgca
    18361 acttctggtg cgcgtcggca acgttaatac attcgaactg atccatgcgt ctctctttct
    18421 ttacaaacaa gtgggcaaat ttaccgcaca gtttacgtcg aagcggcaga taaacgccat
    18481 aatgttatac atatcactct aaaatgtttt ttcaatgtta cccaaagcgc gattctttgc
    18541 taatatgttc gataacgaac atttatgagc tttaacgaaa gtgaatgagg gcagcatgga
    18601 aaccaaagat ctgattgtga taggtggcgg catcaatggt gctggtatcg cggcagacgc
    18661 cgctggacgc ggtttatccg tgctgatgct ggaggcgcag gatctcgctt gcgcgacctc
    18721 ttccgccagt tcaaaactca ttcacggtgg cctgcgctac cttgagcact atgaattccg
    18781 cctggtcagc gaggcgctgg ctgaacgtga agtgctgctg aaaatggctc cgcatatcgc
    18841 cttcccgatg cgttttcgcc tgccacatcg tccgcatctg cgcccggcgt ggatgattcg
    18901 cattggtctg tttatgtacg atcatctggg taaacgcacc agcctgccgg gatcaactgg
    18961 tttgcgtttt ggcgcaaatt cagtgttaaa accggaaatt aagcgcggat tcgaatattc
    19021 tgactgttgg gtagacgacg cccgtctggt actcgccaac gcccagatgg tggtgcgtaa
    19081 aggcggcgaa gtgcttactc ggactcgcgc cacctctgct cgccgcgaaa acggcctgtg
    19141 gattgtggaa gcggaagata tcgataccgg caaaaaatat agctggcaag cgcgcggcct
    19201 ggttaacgcc accggcccgt gggtgaaaca gttcttcgac gaggggatgc atctgccttc
    19261 gccttatggc attcgcctga tcaaaggcag ccatattgtg gtgccgcgcg tgcataccca
    19321 aaagcaggcc tacattctgc aaaacgaaga taaacgcatt gtgttcgtga tcccgtggat
    19381 ggacgagttt tccatcatcg gcaccaccga tgtggaatac aaaggtgatc cgaaagcagt
    19441 gaagattgaa gagagtgaaa tcaattacct gctgaaagtg tataacgcgc actttaaaaa
    19501 gcagctaagc cgtgacgata tcgtctggac ctactccggt gtgcgtccgc tgtgtgatga
    19561 tgagtccgac tcgccgcaag ccattacccg tgattacacc cttgatatcc atgatgaaaa
    19621 cggcaaagca ccgctgctgt cggtattcgg cggtaagctg accacctacc gaaaactggc
    19681 agaacatgcg ctggaaaaac tgacgccgta ttatcagggt attggcccgg cgtggacgaa
    19741 agagagcgtg ctgccgggtg gtgccattga aggcgaccgc gacgattacg ccgctcgcct
    19801 gcgccgccgt tacccgttcc tgactgaatc gctggcacgt cactacacgc gcacttacgg
    19861 cagcaacagc gagctgctgc tcggcaatgc gggaacggta agcgatctcg gggaagattt
    19921 cggtcatgag ttctacgaag cggaactgaa atacctggtg gatcacgaat gggtccgccg
    19981 caccgacgat gccctgtggc gtcgcacaaa acaaggcatg tggctgaatg cggatcaaca
    20041 atctcgtgtg agtcagtggc tggtggaata tacgcagcag aagttgtcgc tggcgtcgta
    20101 aattaacgta aggtgatcag gtcagatttc aatctggcct gagactgatg acaaacacaa
    20161 aactgcctga tgcgctacgc ttatcaggcc tacgtggttt atgcaatata ttgaatttgc
    20221 atggtcttgt aggccagata agacgttcac gtcgcatccg gcatgaacaa agcgcacttt
    20281 gtcaaaaatc taacctactt tttaattcag gaaattaccg caaagcccac gccatcatac
    20341 ccaacgtaac aaaactcagg cacgttcccc actcgccccg agaaaaatag cattaatgcg
    20401 cccagcgtca gcataaaaat attgagcaga aggtaaattg ttggcgtaaa gagtaaattg
    20461 caaacctcag gaatagcggc aagcccagcc cccagcatgg caataaccgc tgcaaaaaat
    20521 tgcgtcagta ttgggatgcc gaaaaaagtc attaccagcg aggtcaaaat ccattttcta
    20581 ttttgcatta ttctttccat tctttctgaa tggcgaaatt atactccccg agtccccttg
    20641 ccccttctgg acacttttcc gaaatgatgg cgaaaaaaac gggacccttt ggc
//
LOCUS       DAJTUI010000070        20602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTUI010000070 DAJTUI010000000
VERSION     DAJTUI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.070"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            86..385
                     /locus_tag="OO464_002743"
     CDS             86..385
                     /locus_tag="OO464_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6227833.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            395..919
                     /gene="ygaP"
                     /locus_tag="OO464_002744"
     CDS             395..919
                     /gene="ygaP"
                     /locus_tag="OO464_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6227834.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(966..1370)
                     /gene="stpA"
                     /locus_tag="OO464_002745"
     CDS             complement(966..1370)
                     /gene="stpA"
                     /locus_tag="OO464_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6227835.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2038..2487
                     /gene="alaE"
                     /locus_tag="OO464_002746"
     CDS             2038..2487
                     /gene="alaE"
                     /locus_tag="OO464_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6227836.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2524..2868)
                     /locus_tag="OO464_002747"
     CDS             complement(2524..2868)
                     /locus_tag="OO464_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6227837.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3020..3250
                     /locus_tag="OO464_002748"
     CDS             3020..3250
                     /locus_tag="OO464_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227838.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3597..3842
                     /gene="nrdH"
                     /locus_tag="OO464_002749"
     CDS             3597..3842
                     /gene="nrdH"
                     /locus_tag="OO464_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6227839.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3839..4249
                     /gene="nrdI"
                     /locus_tag="OO464_002750"
     CDS             3839..4249
                     /gene="nrdI"
                     /locus_tag="OO464_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR6227840.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4222..6366
                     /gene="nrdE"
                     /locus_tag="OO464_002751"
     CDS             4222..6366
                     /gene="nrdE"
                     /locus_tag="OO464_002751"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6227841.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6376..7335
                     /gene="nrdF"
                     /locus_tag="OO464_002752"
     CDS             6376..7335
                     /gene="nrdF"
                     /locus_tag="OO464_002752"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR6227842.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7690..8892
                     /gene="proV"
                     /locus_tag="OO464_002753"
     CDS             7690..8892
                     /gene="proV"
                     /locus_tag="OO464_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6227843.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8885..9949
                     /gene="proW"
                     /locus_tag="OO464_002754"
     CDS             8885..9949
                     /gene="proW"
                     /locus_tag="OO464_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6227844.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10007..10999
                     /gene="proX"
                     /locus_tag="OO464_002755"
     CDS             10007..10999
                     /gene="proX"
                     /locus_tag="OO464_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6227845.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11191..12370
                     /locus_tag="OO464_002756"
                     /pseudo
     CDS             11191..12370
                     /locus_tag="OO464_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12494..13231
                     /gene="ygaZ"
                     /locus_tag="OO464_002757"
     CDS             12494..13231
                     /gene="ygaZ"
                     /locus_tag="OO464_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6227846.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13221..13556
                     /gene="ygaH"
                     /locus_tag="OO464_002758"
     CDS             13221..13556
                     /gene="ygaH"
                     /locus_tag="OO464_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6227847.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13647..14177
                     /gene="emrR"
                     /locus_tag="OO464_002759"
     CDS             13647..14177
                     /gene="emrR"
                     /locus_tag="OO464_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6227848.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14304..15476
                     /gene="emrA"
                     /locus_tag="OO464_002760"
                     /pseudo
     CDS             14304..15476
                     /gene="emrA"
                     /locus_tag="OO464_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15493..17031
                     /gene="emrB"
                     /locus_tag="OO464_002761"
     CDS             15493..17031
                     /gene="emrB"
                     /locus_tag="OO464_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6227849.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17095..17610)
                     /gene="luxS"
                     /locus_tag="OO464_002762"
     CDS             complement(17095..17610)
                     /gene="luxS"
                     /locus_tag="OO464_002762"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6227850.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17760..19316)
                     /gene="gshA"
                     /locus_tag="OO464_002763"
     CDS             complement(17760..19316)
                     /gene="gshA"
                     /locus_tag="OO464_002763"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6227851.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19389..19817)
                     /locus_tag="OO464_002764"
     CDS             complement(19389..19817)
                     /locus_tag="OO464_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6227852.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19814..20380)
                     /gene="yqaB"
                     /locus_tag="OO464_002765"
     CDS             complement(19814..20380)
                     /gene="yqaB"
                     /locus_tag="OO464_002765"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6227853.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4972 a         5270 c         5146 g         5214 t
ORIGIN      
        1 tttactctca tttacgctgc tgaggctggt atatgttgca tttttagttt agagtttact
       61 taatttagaa aatacttaaa taaatatgac tgaactcgcg caattacagg ccagtaccga
      121 acaggcagcg gccttattga aagcaatgag ccaccctaaa cggttgctga ttctgtgcat
      181 gcttagcggt tcccccggca ccagcgcggg agagctgacg cgcattaccg gactgagcgc
      241 ctctgcgaca tcacagcatc tcgctcgtat gcgggacgaa gggcttatca acagccaacg
      301 ggatgcccaa cgcattctat attccattaa aaatgaggcg gtaaatgcca ttatcgccac
      361 cctgaaaaat gtctattgtc cgtaaggagt cactatggct ttgacaacca tttcgccgca
      421 tgatgcacaa gaattaatcg cacgcggcgc aaagttaatc gatattcgtg acgctgatga
      481 atatcttcgt gaacatattc ctgaagcaga tctggctcca ttatccgtgc tggaacagtc
      541 aggtcttcca cctaaattac gtcgcgagca aattatattc cactgccagg caggtaaacg
      601 caccagtaat aacgccgata aattagcagc gattgccgcc cccgcagaaa tatttttact
      661 cgaagatggg attgatggct ggaaaagagc tggattgcca gtagcggtaa ataaatctca
      721 acccttgccg ttaatgcgcc aggtgcagat cgctgcgggt ggtttaatat taaacggcgt
      781 tgtactgggt tataccgtaa atagcggttt cttcttatta agtggctttg ttggtgccgg
      841 gttactgttt gcaggaatca gcggtttttg tggaatggca aggttgttag ataagatgcc
      901 gtggaaccaa cgagcttgag aagcgacgcc ggacgcgccc tagcagcgac atccggcctc
      961 agtaattaga tcaggaaatc gtcgagagat ttaccttctg ccagcgcctg agcaattggc
     1021 ttcggtgtac ggccctgacc ggtccaggtt ttagtttcac cgttaacatc agtgaattta
     1081 tatttcgccg gacgcggctg gcgtttttta ccagcacgtg gtgcagcagc agagctatta
     1141 cccaataact cttccgggtt aattccgtca gctttcatca gctccagcca ggtgctaatt
     1201 ttttcctgac gctcagccag ttcacgctgc tgctgttctt cttcttcacg tctttcttta
     1261 gtgacaaccc tgaatttttc gagcatttct tcaagaacgt caatggagaa ttcgcgagcc
     1321 atcgcacgga gggtgcgaat gttatttaaa ctttgtaaca ttacggacat aataaagaaa
     1381 accttttaac gccaaaacaa aaaagtattt cgtcgcgtag tgtaagcgat cacttattat
     1441 atttccacaa aatttaatct atttacagtc ctgcgcgaga ttatttcagc tgttattttc
     1501 tgaaactctc agaataattt caagattata tattagccat tttgatgagt aatttaggtt
     1561 attggtgaac agagtggcgc tctctgctcg ctaactccgc aagcctgaca aaaccgaagt
     1621 gaataaaaat gcaatataac agaataaaag aaaacacccc tcactggtgc tttctgctgt
     1681 taatgtacat tttctggcat caaaaatgct gtaaatgacc cggtcatgac aaacaccgaa
     1741 ttctctacgc ttatagcgtt acctcccccc caaactttca ttagtgaaat taaaggcaag
     1801 atcatatcta attgatttaa ttgaaaataa atcatattgg catgtaaaaa tccacaatgt
     1861 acaaaaaacg agccgttacg gaatatttta tctacaaaaa ctaactaaat aaaaattttt
     1921 cactaattga ttagtcatag acagcgatat acgctatgca aaaatgcaga tggcaatgag
     1981 atccactgct ttcatctcca ttaacatccc attacgcttt tattaaggag cattagcatg
     2041 ttctcaccgc agtcacgctt gcgtcatgca gttgcagata cgttcgcgat ggttgtttac
     2101 tgttctgtcg tgaacatgtg tattgaagtt ttcctctccg gaatgagctt cgaacagtct
     2161 ttttattcca gattggtagc gattccggtg aacatcttaa ttgcatggcc atacggtatg
     2221 taccgcgatc tgtttatgcg cgcggcacgc aaagttagcc cgtcgggctg gataaaaaat
     2281 ctggctgata tcctggctta tgtgacgttc cagtcaccgg tgtatgtggc gatcttgtta
     2341 gtggtgggcg cagactggca tcagattatg gcggcggtca gttcaaacat cgttgtttcg
     2401 atgttgatgg gggcggttta tggctacttc ctcgattatt gccgccgact gtttaaagtc
     2461 agccgttacc agcaggtaaa agcctgactc ttcttttcgc gactggcatc gccagtcgcg
     2521 tctttaactg gcttcgccaa ataaaccatt caaataacgt tcaagcgcaa cacgcgaact
     2581 aaagccatgc gggataccgt aatgctcgtg tcgctcgcct gctaaataga gcggaaattg
     2641 ctggtaatga tcgcaagttt taatatcgga agcgggttca gccagtgttg aactgcgttc
     2701 ttttaatgtt ggatgaatta ccagagcggt acgtcccatt cgcgcttcgc gattaacata
     2761 gacataattt tccccacggc ggtaaccata cgctttctgg gttaccacat cgacagtaaa
     2821 accgactttt tcaagtacgc gcgccacctc gtctggtcgt aaatacatat ttgatcctcg
     2881 ttattttcaa ccgcgggcct accttacctg attgcgcatt cacaacgctt tcagaaaagt
     2941 ccataaagcc gcgatgaacc tcagttaaga aaattatggt ctacactgaa aattacatcg
     3001 aattctaatg ggggatcata tgtttaaccg accgaaccgc aacgacgttg atgatggcgt
     3061 gcaggatatc cagaatgatg tcaatcaatt agctgacagc ctcgaatctg tattgaagtc
     3121 ctggggcagc gacgccaaag gggaagctga agccgcacgc agtaaagctc aggcgttgct
     3181 gaaagaaacc agggcgcgaa tgcatggtcg tactcgcgtc cagcaagccg cgcgcgatgc
     3241 cgttggctga gcggattctt ttgttcgtga aagaccctgg tgtagcgtgg gtacagcagc
     3301 tgcggtaggt atttttatcg gcgcactgtt aagcatgcgc aaatcgtagt gcaaaaatga
     3361 taataaatac gcgtctttga ccccgaagcc tgtcttcggg gtttcttttt gcctggtgaa
     3421 tcacaaaaat ccccctcccc cgtcacgctc atatccaggg taatttcgat cactatttgc
     3481 tatatattgt gtagttgaat cttttttcaa ctacatctag tatctctgta tcaacagaga
     3541 gacaacccga cgcgtatcat cgcgccgtat cttcatttta aacggaaata cgaatcatgc
     3601 gcataactat ttatactcgt aacgattgcg ttcagtgcca cgccaccaaa cgggcgatgg
     3661 aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa gcagcggaag
     3721 cgttgcgtgc tcagggcttt cgtcagttgc cggtagtgat tgctggcgat cttagctggt
     3781 ctggtttccg tccggacatg attaaccgtc tgcatccagc gccacacgcg gccagtgcat
     3841 gagccagctc gtctacttct ccagcagctc cgaaaacacg cagcgtttta tcgaacgttt
     3901 aggtctgccc gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg tagacgagcc
     3961 ttacatcctg atcgtgccct cttacggcgg cggcggtacg gctggcgcgg tgccacgaca
     4021 ggtaattcgc tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg ttattgcttc
     4081 tggtaatcgc aactttggtg aggcgtatgg ccgcgccgga gatgtgattg cccggaaatg
     4141 cggcgtgccg tggctgtacc gttttgaact catgggtacg caaagcgata tcgaaaacgt
     4201 tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag aatgcctgac gcaggaaacg
     4261 atggattacc acgcgctgaa tgcgatgctt aatctttacg atagcgcagg tcgcattcag
     4321 ttcgataaag accgccaggc cgttgacgcc tttattgcga cgcatgtgcg tccgaacagt
     4381 gtgaccttca gtagccagca gcagcgcctg aactggctgg tcaacgaagg ttactatgat
     4441 gaaagcgttc ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc ccacgcacac
     4501 accagcggtt ttcgtttcca gacattcctc ggggcatgga agttttacac cagctatacg
     4561 ttgaagacat tcgacggtaa acgttatctg gaagattttg ccgatcgagt cacgatggtg
     4621 gcgctgacgc tggcacaagg cgatgagacg ctggcgttgc aactgaccga tgaaatgctg
     4681 tcaggacgct ttcagccagc cacgccaaca ttcctcaact gcggtaagca gcagcgcggc
     4741 gaactggttt cctgtttttt gctgcgtatt gaagacaata tggagtcgat tggtcgggcg
     4801 gtaaattccg cactgcagct gtcgaaacgc ggcggcggcg tagcattttt gctgtcgaat
     4861 ctgcgggaag cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg cgtaattccg
     4921 gtgatgaaaa tgttggaaga cgcattttcc tatgccaacc aactcggcgc gcgtcagggg
     4981 gcaggtgcag tctatttaca tgctcatcat cccgatattc tgcgttttct cgacacgaaa
     5041 cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt cgctgggcgt ggtgatcccg
     5101 gatatcactt tccatctggc aaaagagaat gcgcagatgg cgctgttttc gccctatgac
     5161 gttgagcgag tttatggcaa gccgtttgcc gatatcgcca ttagcgaaca ctatgacgaa
     5221 ctggttgccg atgaacgcat ccgcaaaaaa tacctcaacg cccgtgattt cttccagcga
     5281 ctggcagaaa tccagtttga gtccggctat ccctacatca tgtatgaaga cacagtaaac
     5341 cgtgctaacc ctatcgccgg gcgcataaat atgagtaatc tctgctcaga aattttgcag
     5401 gttaacagcg cctcagagta tgacgagaat ctcgactatg cccgcacagg ccatgatatt
     5461 tcctgcaatt taggttcgtt gaatattgcg cacaccatgg attcccccga tttttcccgc
     5521 acggtagaga ctgccgtgcg cggtttaacg gcggtgtcag atatgagtca tatccgcagc
     5581 gtgccgtcca tcgaagccgg aaatgccgcc tcgcacgcca tcggactggg gcagatgaat
     5641 ttacatggtt atctggcgag ggaaggcatc gcttatggtt cgccggaagc cctggatttc
     5701 accaatctct atttctatac catcacctgg cacgcactgc gtacctcgat gttgctggcg
     5761 cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac gctatgccag tggtgaatat
     5821 ttcagccaat atctgcaagg taactggcag ccgaaaacgg cgaaagttgg cgaactgttt
     5881 gcccgcagct gtattacgtt acctacccgt gagatgtggg cgcaactgcg cgacgacgtg
     5941 atgcgctacg gcatatacaa ccagaatctc caggcggtgc cgccaaccgg gtctatctct
     6001 tatatcaacc atgctacgtc gagtattcat ccgattgtgg cgaaagtaga gatacgcaaa
     6061 gagggcaaaa caggacgcgt ttactaccct gccccgttta tgactaacga aaatctggcg
     6121 ctgtatcagg acgcttacga aattggcgca gaaaagatca tcgacaccta cgcggaagcg
     6181 actcgccatg tcgatcaggg gctgtcgctg acgctgtttt tccccgatac cgccaccact
     6241 cgcgatatca acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac gctctattac
     6301 atccgcctgc gtcagatggc gctggaaggc actgaaattg aaggctgcgt ctcctgtgca
     6361 ctttaaggaa tatctatgaa actctcacgt atcagcgcca tcaactggaa caagatatct
     6421 gacgataaag atctggaggt gtggaatcgc ctgaccagca atttctggct accagaaaag
     6481 gtgccgctgt cgaacgatat tcccgcctgg cagacattaa ctgtcgtaga acaacaactg
     6541 acgatgcgcg tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt tatcggcgcg
     6601 ccttcgctga tgcccgatgc gctcacgcct catgaagagg cggtattatc gaatatcagc
     6661 tttatggaag cggttcatgc ccgctcttac agttcgattt tctcgacgct gtgccagacc
     6721 aaagatgtcg atgccgccta cgcctggagt gaagaaaacg caccgttgca gcgaaaagct
     6781 cagattattc agcaacatta tcgcggtgat gatccgctga aaaagaaaat cgccagtgtg
     6841 tttcttgaat cttttttgtt ctattccggt ttctggctgc cgatgtattt ttccagccgc
     6901 ggaaagctga ccaataccgc ggacctgatc cgtctgatta tccgcgatga agcagtccac
     6961 ggttactaca taggctataa atatcagaaa aacatggaaa agatatctct gggacaacgt
     7021 gaagagttga agagtttcgc cttcgatttg ttgctggaac tctacgacaa cgagttgcaa
     7081 tacaccgatg agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc gtttctctgt
     7141 tacaacacca ataaggcttt gatgaatctg ggctacgaac cgttatttcc cgcagaaatg
     7201 gcggaagtga atccggcaat cctcgccgcg ctttcgccga atgccgatga aaatcacgat
     7261 ttcttttccg gttcaggctc ctcttatgtg atggggaaag cggttgaaac agaagatgaa
     7321 gactggaatt tctgagggtg ttattttcaa aaatatcact acccgcagca gggaaataat
     7381 tcccgccaaa tagcttttta tcacgcaaat attctgtagt gatctacact gatactctgt
     7441 tgcattattc gcctgaaact acaatattca ggcgtttttt cgctatctat gacaaaaaat
     7501 atcaactttt ctcgatttgc tctcagccct tatatcacgg aaaattccgg cgatttgctc
     7561 gcatcaatat tcatgccaca tttgccctca ggggttgcct cagattctca gtatgttagg
     7621 gtagaaaaaa gtgactattc ccattgggta atatatcgac ataaacaaat aaagaaatca
     7681 ttctattgca tggccattaa attaaaaatt aaaaatcttt ataaaatatt tggtgagcat
     7741 ccacagcgag cgttcaaata tatcgaacaa ggactttcaa aagaacaaat tctggaaaaa
     7801 actgggctat cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg cgagatattt
     7861 gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg tacgccttct caatcgcctg
     7921 attgaaccca cccgcgggca agtgctgatt gatggtgtgg atattgccaa aatatccgac
     7981 gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc ctttgcctta
     8041 atgccgcata tgaccgtgct ggacaatact gcgttcggta tggaattggc cggaattaat
     8101 gccgaagaac gccgggaaaa agcccttgat gcactgcgtc aggtcgggct ggaaaattat
     8161 gcccacagct acccggatga actctctggc gggatgcgtc aacgtgtggg attagcccgc
     8221 gcgttagcga ttaatccgga tatattatta atggacgaag ccttctcggc gctcgatcca
     8281 ttaattcgca ccgagatgca ggatgagctg gtaaaattac aggcgaaaca tcagcgcacc
     8341 attgtcttta tttcccacga tcttgatgaa gccatgcgta ttggcgaccg aattgccatt
     8401 atgcaaaatg gtgaagtggt acaggtcggc acaccggatg aaattctcaa taatccggcg
     8461 aatgattatg tccgtacctt cttccgtggc gttgatatta gtcaggtgtt cagtgcgaaa
     8521 gatattgccc gccggacacc gaatggctta attcgtaaaa ctcctggctt cggcccacgt
     8581 tcggcactga aattattgca ggatgaagat cgcgaatatg gctacgttat cgaacgcggt
     8641 aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac gcagcagcaa
     8701 ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac gcctcttagc
     8761 gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga cgaggaccaa
     8821 cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg tgagggggta
     8881 aataatggct gatcaaaata atccgtggga taccacgcca gcggcggaca gtgccgcgca
     8941 atccgcagac gcctggggta caccgacgac tgcaccggct gacggcggtg gtgctgactg
     9001 gctgaccagt acgcctgcgc caaacgtcga gcattttaat attctcgatc cgttccataa
     9061 aacgctgatc ccgctcgaca gttgggtcac tgaagggatc gactgggttg ttacccattt
     9121 ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat atcctcaacg gtttccagca
     9181 attgctgctg ggtatgccag cgccagtggc gattatcgtt ttcgctctca tcacctggca
     9241 gatttccggg gtcggaatgg gcgtggcgac gctggtctcg ctgattgcca tcggcgcaat
     9301 cggtgcctgg tcgcaggcaa tggtgactct ggcgctggtg ttaactgccc tgctgttctg
     9361 tatcgtcatc ggtttgccgt tggggatatg gctggcgaga agtccgcgag cggcgaaaat
     9421 tattcgtcca ctgcttgatg ccatgcagac cacgccagcg tttgtttatc tggtgccaat
     9481 cgtcatgcta tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca tctttgcgct
     9541 gccgccgatt atccgtctga ccattctggg gattaaccag gttccggcgg atctgattga
     9601 agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc agttaccgct
     9661 ggcgatgccg accattatgg cgggcgttaa ccagacgctg atgctggccc tttctatggt
     9721 ggtcattgcc tcgatgattg ccgtcggcgg gctgggtcag atggtacttc gcggtatcgg
     9781 tcgtctggat atggggcttg ccaccgttgg cggcgtcggg attgtgatcc tcgccattat
     9841 cctcgatcgc ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca accgtcgctg
     9901 gtacaccact ggccctgttg gtctgctgac ccgcccattc attaagtaac tctgcacttg
     9961 cccggtgacg ccgggcatta tcaccctgcc aaaaaaagga ataacaatgc gacatagcgt
    10021 actttttgcg acagcgtttg ccacgcttat ctctacacaa acttttgctg ccgatctgcc
    10081 gggcaaaggc attactgtta atccagttca gagcaccatc actgaagaaa ccttccagac
    10141 gctgctggtc agtcgtgcgc tggagaaatt aggttatacc gtcaacaaac ccagcgaagt
    10201 ggattacaac gttggctaca cctcgcttgc ttccggcgat gcaaccttca ccgccgtgaa
    10261 ctggacgcca ctccatgaca acatgtacga agctgccggt ggcgataaga aattttatcg
    10321 tgaaggggta tttgttaacg gcgcagcaca gggttacctg atcgataaga aaaccgccga
    10381 ccaatacaaa atcaccaaca tcgcacaact gaaagatccg aagatcgcca aactgttcga
    10441 taccaacggc gacggtaaag cggatttaac cggttgtaac cctggctggg gctgcgaagg
    10501 tgcgatcaac caccagcttg ccgcgtatga actgaccaac accgtgacgc ataatcaggg
    10561 gaactacgca gcgatgatgg ccgacaccat cagtcgctac aaagagggca aaccggtgtt
    10621 ttattacacc tggacgccgt actgggtgag taatgagctg aagccaggga aagatgtggt
    10681 ctggttgcag gtgccgttct ccgcactgcc gggcgataaa aatgccgata ccaaactgcc
    10741 gaatggcgcg aattacggct tcccggtcag caccatgcat atcgttgcca acaaagcctg
    10801 gaccgagaaa aatccggcgg cagccaaact gtttgccatt atgcagttgc cagtggccga
    10861 tattaacgcc cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg atattcaggg
    10921 ccacgttgat ggctggatca aagcccacca gcagcagttc gatggctggg tgaatgaggc
    10981 gctggcagcg cagaagtaat ttttattcgg gcggataagg cgttttcgcc tcatccgccg
    11041 ctctgtacac aatgcctgat gcgacgctga cgcgtcctat catgcctaca aaccacatcg
    11101 cacatttcaa caagctatct ttcatcccat attcatcaac atccgctatt attgatttcc
    11161 agcttaatca tcacctgatg aacaaaaata atgactaaac ctaatcatga gcttagcccg
    11221 gcgctgatcg tgctgatgtc tatcgccacc ggtctggcgg tagccagtaa ctattacgcc
    11281 cagccattgc tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc ggcaggcttt
    11341 attgttaccg ccgcgcagtt gggctatgcc gcaggtctac tgtttcttgt tcccctcggt
    11401 gatatgtttg aacgccgccg cctgattgtc tcgatgacct tactggcagc gggcggtatg
    11461 ttaatcactg ccagcagtca gtcactggcg atgatgatcc tcggtacggc attaaccggt
    11521 ttattctcag tcgtggcaca aattctggtt ccgctggcag cgacgctggc ttcaccggat
    11581 aaacgcggca aagtggttgg caccattatg agcggtctgc tgttggggat attgctggca
    11641 cgaaccgttg ccgggttact ggcaaacctc ggcggctggc gcaccgtctt ttgggttgct
    11701 tcggtgttaa tggcactgat ggcgctggca ttatggcgtg gtctgccaca aatgaaatca
    11761 gaaacccacc tcaactaccc acagttgttg ggttccgttt tcagcatgtt catcagcgat
    11821 aagatcctgc gcacccgcgc gttgctgggc tgcatgacct ttgccaactt cagcattctc
    11881 tggacctcaa tggccttttt gcttgccgct ccacctttta actacagcga tggtgacatt
    11941 ggtctgtttg ggcttgcggg agctgccgga gcgttgggcg ctcgtccggc gggcggtttt
    12001 gccgacaagg gcaaatcgca ccacaacttt cggtctgctt ctgctgttac tttcatggct
    12061 ggcaatctgg tttggtcaca cttccgtact ggcgttgatt atcggcattc tggtgctgga
    12121 cctcaccgta cagggcgtgc atatcactaa ccagacggta atttatcgaa tacatcctga
    12181 tgcgcgtaat cgcctgaccg caggttacat gaccagctac tttattggcg gtgccgccgg
    12241 ttcgctaatt tcagcctcag cctggcaaca tggcggttgg gctggcgttt gtctggctgg
    12301 cgcgacgatt gccctggtta acttactggt ctggtggcga ggttttcatc gtcaggaagc
    12361 cgcaaattaa gcaatcagca accgttttcg tggctttaca cttataaggg tgttaagaag
    12421 cccatcagtc tgataaggtt aagatattca ttcagtctat ttataatatt aacaatcgtt
    12481 aagcgtacac tctatggaaa gccctactcc acagcctgct cctggttcgg cgaccttcat
    12541 ggaaggatgc aaagacagtt taccgattgt tattagttat attccggtgg cctttgcgtt
    12601 cggtctgaat gcgacccgtc tgggattctc tcctctcgaa agcgtttttt tctcctgcat
    12661 catttatgca ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg ggagtagttt
    12721 gtgggttgct gcactgagcg tcatggcaat ggatgttcgc catgttttgt atggcccgtc
    12781 actgcgtagc cgtattattc agcgtctgca aaaatcgaaa accgccctat gggcgtttgg
    12841 cctgacggat gaggtttttg ccgccgccac cgcaaaactg gtacgcaata atcgccgctg
    12901 gagcgagaac tggatgatcg gcattgcctt cagttcatgg tcatcgtggg tatttggtac
    12961 ggtaataggg gcattctccg gcagcggctt gctgcaaggt tatcccgccg ttgaagctgc
    13021 attaggtttt atgcttccgg cactctttat gagtttcctg ctcgcctctt tccagcgcaa
    13081 acaatctctt tgcgttaccg cagctttagt tggtgccctt gcaggcgtaa cgctattttc
    13141 tattcccgtc gccattctgg caggcattgt ctgtggctgc ctcactgcgt taatccaggc
    13201 attctggcaa ggagcgcccg atgagctatg aggttctgct gcttgggtta ctggttggcg
    13261 cggcgaatta ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat gcccgcccaa
    13321 ccaaacgtgg cgcggtaggt attttgctcg acaccattgg catcgcctcg atatgcgctc
    13381 tgctggttgt ctctaccgca ccagaagtga tgcacgatac acgccgtttc gtgcccacgc
    13441 tggtcggctt cgcggtactg ggtgccagtt tctataaaac acgcagcatt atcatcccaa
    13501 cactgcttag tgcgctggcc tatgggctcg cctggaaagt gatggcgatt atataaccca
    13561 caagaatcat ttttctaaaa caatacattt actttatttg tcactgtcgt tactatatcg
    13621 gctgaaatta atgaggtcat acccaaatgg atagttcgtt tacgcccatt gaacaaatgc
    13681 taaaatttcg cgccagccgc cacgaagatt ttccttatca ggagatcctt ctgactcgtc
    13741 tttgcatgca catgcaaagc aagctgctgg agaaccgcaa taaaatgctg aaggctcagg
    13801 ggattaacga gacgttgttt atggcgttga ttacgctgga gtctcaggaa aaccacagta
    13861 ttcagccttc tgaattaagt tgtgctcttg gatcatcccg taccaacgcg acgcgtattg
    13921 ccgatgaact ggaaaaacgc ggttggatcg aacgtcgtaa aagcgataac gatcgccgct
    13981 gcctgcatct gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt ttaccaccgc
    14041 agcataactg cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa gatcagctcg
    14101 agcaaatcac ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac ggtgtggttc
    14161 tcgaagcgat gagctaacgc gtcatctcgc tcaaaaatcc agatttataa aagaaaaaat
    14221 gaccggccag catcgcaaca tgctggcctt tttggcaagc aggtcggctc agccgatgag
    14281 ttaagaagat cgtggagaac aatatgagcg caaatgcgga gactcaaacc ccgcagcaac
    14341 cggtaaagaa gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg ctctttataa
    14401 ttattgccgt agcgataggg atttattggt ttttggtact gcgtcatttc gaagaaaccg
    14461 atgacgcata cgtggcaggg aatcagattc aaattatgtc tcaggtgtct ggcagcgtga
    14521 cgaaagtctg ggccgataac accgattttg taaaagaagg cgatgttctg gtcactctcg
    14581 acccgacaga tgctcgccag gcgtttgaaa aagccaaaac tgcactggct tccagcgttc
    14641 gccaaaccca ccagctgatg attaacagca aacagttgca ggcgaatatt gaggtgcaaa
    14701 aaatcgccct cgcgaaagca caaagcgact acaaccgccg tgtgccgctg ggcaatgcca
    14761 acctgattgg tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt gcccaggcgc
    14821 aactggacgt cgctattcaa caatacaatg ccaatcaggc gatgattctg gggactaaac
    14881 tggaagatca gcctgccgtg caacaggctg ccaccgaagt acgtaacgcc tagctggcgc
    14941 tggagcgtac ccgtattgtc agtccgatga ccggttatgt ttcccgtcgt gccgtgcaac
    15001 cgggtgcgca aattagccca acgacgccgc tgatggcggt cgttccagcc accaatatgt
    15061 gggtggatgc caactttaaa gagacgcaga ttgccaatat gcgcatcggt cagccggtca
    15121 ccatcaccac cgatatttac ggcgatgatg tgaaatacac cggtaaagtg gttggtctgg
    15181 atatgggcac aggtagcgcg ttctcactgc ttccggcgca aaatgcgacc ggtaactgga
    15241 tcaaagtcgt tcagcgtcta cctgtgcgta tcgaactgga ccagaaacag ctggagcaat
    15301 acccgctgcg cattggtttg tccacgctgg tgagcgtcaa taccactaac cgtgacggtc
    15361 aggtactggc aaataaagta cgttcaacac cggtcgcggt aagtaccgcg cgtgaaatca
    15421 gcctggcacc tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct ggctaatcca
    15481 gaggtgcgtg tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt cattatgacg
    15541 attgcgctgt cactggcgac attcatgcag gtgctgaact ccaccattgc taacgtggcg
    15601 atccccacta tcgccgggaa tctgggctca tcgctcagcc aaggaacgtg ggtaatcact
    15661 tctttcgggg tggcgaatgc catctcgatc ccgcttaccg gctggctggc aaagcgcgtc
    15721 ggggaagtga aactgttcct ttggtccacc atcgcctttg ctattgcgtc ttgggcgtgt
    15781 ggtgtctcca gcagcctgaa tatgctgatc ttcttccgcg tgattcaggg gattgtcgcc
    15841 gggccgttga tcccgctttc gcaaagtcta ttgctgaata actacccgcc agccaaacgc
    15901 tcgatcgcgc tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg cggcccgatc
    15961 ctcggcggtt atatcagcga taattaccac tggggctgga tattcttcat caacgtgccg
    16021 attggcgtgg cggtggtgtt gatgacactg caaaccctgc gcggacgtga aacccgcacc
    16081 gaacggcggc ggattgatgc cgtggggctg gcactgctgg ttattggtat cggcagcctg
    16141 cagattatgc tcgaccgcgg taaagagctg gactgttttt catcacagga aattatcatt
    16201 cttaccgtgg tggcggtggt ggctatctgc ttcctgattg tctgggagct gaccgacgat
    16261 aacccgatag tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg ctgcttgtgt
    16321 atcagcctcg cgtatatgct ctacttcggc gctattgttc tgctgccgca gttgttgcag
    16381 gaggtctacg gttacacggc gacctgggca ggtttggcct ctgcgccggt agggattatt
    16441 ccggtgattc tgtcgccgat tatcggccgc ttcgcgcata aactggatat gcggcggctg
    16501 gtaaccttca gctttattat gtatgccgtc tgcttctact ggcgtgccta tacctttgaa
    16561 ccaggtatgg atttttgcgc gtcggcctgg ccgcagttta tccaggggtt tgcggtggtc
    16621 tgcttcttta tgccgctgac caccattacg ctgtctggtt tgccaccgga acgactggcg
    16681 gcggcatcga gcctctctaa ctttacgcga acgctggcgg ggtctatcgg cacgtcgata
    16741 accacgacca tgtggaccaa ccgcgagtcg atgcaccatg cgcagttgac tgagtcggta
    16801 aacccgttca acccgaatgc ccaggcgatg tacagtcaac tggaagggct tgggatgacg
    16861 caacagcagg catcaggctg gattgcccag cagatcacca atcaggggct gattatttcc
    16921 gccaatgaga tcttctggat gtcagccggg atattcctcg tactgctggg gctggtgtgg
    16981 tttgctaaac cgccatttgg cgcaggtggc ggcggaggcg gtgcgcacta agtacaacta
    17041 agccagttca tttgaactgg cttttttcaa ttaattgtga agatagttta ctgactagat
    17101 gtgcagttcc tgcaacttct ctttcggcag tgccagttct tcgttgctgt tgatgcgtac
    17161 ctcacgttcc agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt gcatctggta
    17221 agtgccacac tggtagacgt tcagttccgg gatctgattc tgatcctgca ctttcagcac
    17281 gtcttccatt gccgctttcc aggcatcagc aacacgctgc tcatctggcg taccaatcag
    17341 actcatataa aaaccggtgc ggcagcccat tggcgagata tcgataatct ctacaccatt
    17401 accgttaaga tggttacgca taaaaccagc aaacaggtgc tccagggtat ggatccctct
    17461 ttctggcatc acttctttgt tcggcacgca gaagcgcaga tcgaacacgg tgattgcgtc
    17521 gccatgcggg gtgttcattg ttttcgccac ccgaactgca ggcgcttcca tccgggtatg
    17581 atcgactgtg aagctatcta acaacggcat ttagccacct ccggtaattt ttttaaaaat
    17641 tttctgaact ctttcttccc aggcgagtct gagtatatga aagacgcgca tttgttatca
    17701 tcatccctga attcagagat gaaattttgg ccactcccga gtggcctttt tcttttctgt
    17761 caggcgtgtt tttccagcca caccgcaaac ggttcggtat cagcggtttc catttcctgc
    17821 tgacggcgtt cagatgcctc gcgctcggct acaaaatcct cttcgcgcag aatttccagc
    17881 ggctcttcac gcagcagatt acggtaggct tctgcaaacg ctttgcctgt tccgccaata
    17941 ccagtatcaa tcatagacct taagatacgg gcagagaaag tcagatcggg attatcgaag
    18001 caggcaacca gttcatcaca cactttctga tacgcttcgc cgccgttaat gctatccagc
    18061 gtttgcgcga cgcgtttcag atcgcggaac agatctttac ccacctgcag taacgggaac
    18121 tgcgcggttt cgcagccgat acccagcgtc aggcccggtt tgcgaccttc aaggatcacc
    18181 cggttccagt taacgcgcgt acaggcaagt tcgctactgc tcatttccgg tgcatcagcc
    18241 agcgcacacc agaccataaa caggtcgagg aatcgcacct gctgttcatc tacaccaatc
    18301 ggcgagaacg ggttgatgtc cagcgaacgc acttcaatat attcaatgcc gccacgtaac
    18361 agcgcatcag aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat cggcgcatac
    18421 agttcgtttt caatctgcag cacgttgcta ttgatttgca gcctcttacc gtctttctca
    18481 ataccaatct tcgcgtactc ttccgatggc gttttgattg cctgtttaag gcccgctacg
    18541 tactcgtaaa gatcgttgaa ggtaatacca agattgcttt gcgatttatt ggtatagccg
    18601 agatcgctca aacgaagaga ggtcgcatac ggcaggtaat acataccgca ctcggttttc
    18661 tcaaacggca gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc cggagatgca
    18721 ccaaacagat aaggaataac ccaaccgaaa cgatagtaat tgcggataac gcggaaatag
    18781 cccgcagaaa ttttctcttt ggcatcagcg cccgagatat caccgcactt cgcttgccag
    18841 aatgccattg gcaaagagaa attgtagtgc acgccggaaa tggtttgcat cagcgcgccg
    18901 tagcgatttt tcagcccttc acgatacagc gttttaaagc gtccggtgtt agaagtgccg
    18961 tactgtgcca gttcgatgtc ctgaccttct gcgatgtagc atggcatact taacggccac
    19021 atccgctcat cgcccatatt gcgcgccgta taacgatgca gatcgcgcat aaaggtcagc
    19081 atatgttcaa tatcaccatc cactggtgta atgaattcca gcaatgcttc cgcaaaatcg
    19141 gtagtaatcc atttgtgcgt cagtgcggaa cctaatgctt caggatgacc tgttgttgcc
    19201 agtgtgccat cagcattaac acgcaaagtt tcgcgctcca gcccacgctg tatccccttt
    19261 aacgcctgag gatgtttttc cagccaggcc agcgcctgtg atacgtccgg gatcaaattg
    19321 acctcccgcc tgtcaaaatc gttttaatta gcataactgt aatggtgacc atatgtgcag
    19381 gcctacaatt agtgccacca catcatgccc tgaacggtcg ctgctgcaac tgcaacatag
    19441 cgtaacgctt taccaaggca caaaaaaaag attactggcc cccaggagat gcgcatccat
    19501 cccgctaaca gacacagtaa atcgccaacc acgggcatcc agcttaataa tagcgtgact
    19561 gcaccatagc gtttcagcca gccggtagct ttctcttgcc agcgcgatgt tttacgcaat
    19621 ggaaagaaac gcccaaggat aacgttagtt aaccctccaa ggctattacc cattgttgct
    19681 gttaaaacta aaacccaggg atgactgatc ccggaaagca acattgccac cagcacgact
    19741 tcggagttgc cgggtaatag tgtagcgctg agaaaactac tggcaaacaa cgagaaaagc
    19801 gataacgctt cactcacagc aagcgaacat ccacagcgtc catgcctgct gcacgggccg
    19861 cctgaatacc gaaatcggca tcttcaaaga ccacacactg cgtcggttgc acgcccatac
    19921 gctgcgcgca caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg tgatcagcag
    19981 cgacgacggc gtcaaaataa cggcgtaatc ccaggtgcgc cagcaatgcc tcagcgatgg
    20041 cgctttcact ccccgttcct acagccattg ggcgacgacc atgccaactt ttcaccactt
    20101 caacaagagg aagcggctcg acactatcca gcagcatact tcttactgct tctgtttttt
    20161 cacgcgctaa cgcatgcggg tcgagatcgg cctgattcag ctcaataatt gcctgagcaa
    20221 tacgccaggt gggcgatcca ttaagcgcaa tcatcgcctg aatatcgtac tgaagaccgt
    20281 agtgccctaa tacttcgcgc cacgctttac ggtgcgtagg cgccgtatcc aggattgtgc
    20341 catccatatc aaaaattaaa cctgcataac gctcgtacat ggtcttctcg caagtcgaaa
    20401 aatatgacgc tactttagcg taattgcttg attttgtcgc tgatggcgaa ggagaagaaa
    20461 attcaggaaa tggataaagt agttatttag aaatgagata tttttgaagg aaattttttg
    20521 gagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt agccgagtac
    20581 tctatccagc tgagctacgg at
//
LOCUS       DAJTUI010000071        20429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTUI010000071 DAJTUI010000000
VERSION     DAJTUI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.071"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..4267)
                     /gene="mukB"
                     /locus_tag="OO464_002766"
     CDS             complement(<1..4267)
                     /gene="mukB"
                     /locus_tag="OO464_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6227854.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFEL"
     gene            complement(4267..4971)
                     /gene="mukE"
                     /locus_tag="OO464_002767"
     CDS             complement(4267..4971)
                     /gene="mukE"
                     /locus_tag="OO464_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6227855.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(4952..6274)
                     /gene="mukF"
                     /locus_tag="OO464_002768"
     CDS             complement(4952..6274)
                     /gene="mukF"
                     /locus_tag="OO464_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6227856.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6271..7056)
                     /gene="cmoM"
                     /locus_tag="OO464_002769"
     CDS             complement(6271..7056)
                     /gene="cmoM"
                     /locus_tag="OO464_002769"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6227857.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7192..7971
                     /gene="elyC"
                     /locus_tag="OO464_002770"
     CDS             7192..7971
                     /gene="elyC"
                     /locus_tag="OO464_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6227858.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(7948..8841)
                     /locus_tag="OO464_002771"
     CDS             complement(7948..8841)
                     /locus_tag="OO464_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6227859.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(8995..9741)
                     /gene="kdsB"
                     /locus_tag="OO464_002772"
     CDS             complement(8995..9741)
                     /gene="kdsB"
                     /locus_tag="OO464_002772"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6227860.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9738..9920)
                     /gene="ycaR"
                     /locus_tag="OO464_002773"
     CDS             complement(9738..9920)
                     /gene="ycaR"
                     /locus_tag="OO464_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6227861.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(9972..11204)
                     /locus_tag="OO464_002774"
     CDS             complement(9972..11204)
                     /locus_tag="OO464_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6227862.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11241..12227)
                     /gene="lpxK"
                     /locus_tag="OO464_002775"
     CDS             complement(11241..12227)
                     /gene="lpxK"
                     /locus_tag="OO464_002775"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6227863.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12224..13972)
                     /gene="msbA"
                     /locus_tag="OO464_002776"
     CDS             complement(12224..13972)
                     /gene="msbA"
                     /locus_tag="OO464_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6227864.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14009..16268)
                     /locus_tag="OO464_002777"
                     /pseudo
     CDS             complement(14009..16268)
                     /locus_tag="OO464_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16475..16759)
                     /gene="ihfB"
                     /locus_tag="OO464_002778"
     CDS             complement(16475..16759)
                     /gene="ihfB"
                     /locus_tag="OO464_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6227865.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(16919..18592)
                     /gene="rpsA"
                     /locus_tag="OO464_002779"
     CDS             complement(16919..18592)
                     /gene="rpsA"
                     /locus_tag="OO464_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6227866.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18703..19386)
                     /gene="cmk"
                     /locus_tag="OO464_002780"
     CDS             complement(18703..19386)
                     /gene="cmk"
                     /locus_tag="OO464_002780"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6227867.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19559..20323)
                     /gene="ycaL"
                     /locus_tag="OO464_002781"
     CDS             complement(19559..20323)
                     /gene="ycaL"
                     /locus_tag="OO464_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR6227868.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4920 a         5662 c         4955 g         4892 t
ORIGIN      
        1 acaattcaaa cagcgtggcg atagaacgag catccagtcg cgctgcttca tcgaggaaca
       61 gcaggcggca aggagagata tctttaccgc gcaggcggcg agattcatct tcccagcttt
      121 gtaccaccat caccagaatc gacataccgg taccaatcgc ctcaccggtc gacaatgcac
      181 cagactctgc gcgcaaccaa ccatcggaac cacggttaac ctcaacttcc atttccagat
      241 agttgcggta atccagcagt tcttcgccaa tggtctgcgg cgtgcgctgc cccatatcaa
      301 tctgcggatt aagacgctga tacaatttcg ccaacgcttc cgagaaggtc agacggttgc
      361 tgttaaacag atcctgatgc tgctcgtgct gttcagagag cacatccagc agcatggcgt
      421 gcgtttcacg cacgttcacg ttgagacgca cgctgttcac ctgaccaaac gatacgttct
      481 gcaacccctg gttgagcata cggatacggt tctgctcgcg ctgaatggtt ttgcgaatga
      541 tgttcgccac gctgcgggaa ctgatcgcca gtttctgttc acgggaggtt aattcttcgg
      601 tcagacggct aagttcaatc tccatctgtt cgatagcttc caccggatca tcggtacgaa
      661 taatatcctg acgaatacgt tcacgcagat gctgataaac cgccacgaag aactgaattt
      721 tacgctccgg acgtttcgga tcttccgaca tgcgcagcac gtcgcgcaga tgttcgttat
      781 ccgccaccgc cagacgcagc gcacctaacg ccttatccga catggaacgc aaatcatcag
      841 cggagagata agccagctca cgacggtgta agcggcgctc aacgccgtta tctttcacca
      901 tgcgcatcac cgcacaccag cccgctttgg cggtcactac ctgctcgcgc atctcaaagt
      961 aatcccgctc cagcttgcgc agtttgcggg tcaggttgtc catctccgct tcgcagaagg
     1021 taagcgcttt ttccagttga ttgcggcgtg aacggttatt gctcagttgc gcatgcagct
     1081 cgtcacggcg aatacgcgcc cgctcttctg ccccgctatc agcacgcacg ccgatatcct
     1141 gcaattcacg ttgcagatcg ttgagtagct cttttttggt gtcgtaagaa cttttcagcg
     1201 aagccagcac ctggttgtac tgactcaact gcgctgcgtg accgcgcaac gcttcgcgag
     1261 cgcgggtacg ctccgcttcc gcctgttcca gacgttcacg cagtttttcg ttgagatcgc
     1321 tgttaccgct aagcatttct gccgagtcag aatagctaaa gtgcgcacga cgctgcacca
     1381 cttccgtcag ggcaaacgct tgctgacggg catcgcgctg catctgctga gagtacgcgt
     1441 aatcttcttt taactgttcg aactgttccg ggtcgctctg caataccgaa acgatcggtt
     1501 ccagtttcgc cagttgattg ccaaactgct gaacaaaacg cgcggcttcc tgggcttcat
     1561 ccagacgttc gcggatttca tcgacgcgat ccgccaggct gtcatcagcc aacaggttga
     1621 gacgcggcag aatgcggttc agcgccgtaa cgccttcttt cgcctgctca aactgaatac
     1681 gctgctgctg gttatcattt tcatgattac tcaacgcccg ctccagttcg actcgacggc
     1741 tgttcagttg acggatttct gcttccgggt cagactcaaa cgccaccgcc agatgactgc
     1801 cgataaagcg gctgaacgcc tgatgcagac gctgggtttt ctgtacatca aaggagagcg
     1861 tggcgaagcg ttcggaaagc acttcacgct cggcatggag gctttcaata cggctttcac
     1921 gcgcagcacg accaaacagc ggcacttccg ggaaacgtga ataacgccac tgtcgatcgg
     1981 cgattttcac caccaccgct ttttccagct catcaacgct gaacacgcta tcatcgaatg
     2041 actgcggatc cccttcgatc aaatagagat cttccgggca atcggtcaag ccttccaggt
     2101 gttcggttac ctgtgacaga tctggcacca cgatggcgtg gcgtgacggg ccatacagag
     2161 ccgagaagta cggcgcatct tccaggctaa cgtcgtcata aatttctgac agcagcacac
     2221 caccaaaacg ctccgccagc gcgttcagac gctgatcttc agagccgcca ggctggctta
     2281 aacgttcgat ctcttcatcg acggcgtttt tgcgcgcgcc tacttcatcg cgttcaacaa
     2341 tcgcctcgcg ctcacgctcc agcaactgtt gcagatattc agtgacgtcc tggctggagg
     2401 agaactcttc gccgcactgt tcactcaact ggttgagact gttttgcgct gccagccaaa
     2461 ccggtgcacg ctgcatcaaa ctctgaatgc gagactgcag ctgttcctgt tcctgccgca
     2521 gtgccatccg ctcttcacgg gcgttagata cgctatcgga aagagaggca atgcgtgctt
     2581 ccagctcctg atgcagagct tccagttcat caatatcaaa attcttgccc tgacgtttgc
     2641 agaaatctgc cagcagacgc tcagcttctt gctgctcacg cagacgctgt tccagttcgc
     2701 ttaagcgcat ccgcaacggc tgaacctgct ctgccaggtg acgctgatca accccttcgc
     2761 gcaatagttc gcgagcgaca tcccacgcct cgttacgcgc cagcgggccg ttgattgcca
     2821 ccaccagctg ataagcctgc tcaaactggc tgtgcgcggt ttgcgccatg ctcattttct
     2881 gctcaagaga gagcattttt tcagtcgctt ccagctcttt cgcctggaag gtttccagcc
     2941 attcggcggc gctgtcggcg gttaagtccg gcagatggca cagttctttg gcacgattaa
     3001 gcgcagcaat cgcctggtta tactggatcg cgcgcgtttg ctggacgtcc agcgcctgct
     3061 ggtagtcagc aagctggctt ttcagctcgt ccacttccag ttcggcagct tccgcgcgag
     3121 cctcattctc ttcctggcgt tcgatggctt ctgccaccac ttcattttgc tcttccagac
     3181 ggatctgcag ctcatcgaga tccacttcgt agcgttcgat cttctcctgc tgacgcaatg
     3241 cggtttgcac caggttcagg tgatcgctgg ccgcctgata atccgcttcc agatcacctt
     3301 cggcaccgtt gtgctctgcc agctcacgcg ccatatcgac gtgtttgtac tgctcagccg
     3361 ccagttgctg acgcgaagta tgtagctcgc gacgaaactc cagggctttg tcgagatgga
     3421 cacggcgctc gttggcgtga cgcatgtagt ccgccgccac gtagttggta gcttcgctga
     3481 tcagatgctt aaacaggtca cggtccgact gggtgacacg aatcgcttcc agcgtcatgc
     3541 ggttttcacg cagcgccgct tccatgtcct ggaacgcttt acgcacgccg ctgttttccg
     3601 gcaacaggta gtcgcgcaga gaacgggtaa tggcactgga gatcccgcca tacagcgaag
     3661 cttcgatcag acgatagaat ttgctacggt cagatgcgga gcgcagacga cgcgcgatga
     3721 tgcccagatc gaacatcagc gagtggtaat cggtaatgga gttgaactgt ttaaactgca
     3781 cgccttccat cgcctcgagc ttgtctttca gctcgttaag cggcagcacg cgcgcctggc
     3841 gttcgttcag ggtttcggtc accagctgtg tcggctgcac cgacatcggc agtccctgaa
     3901 tggcaaatgg tttgatatcc actttacgat cgcgtccggc aacctgttgc agacgcacac
     3961 cgaccaccac gcgctggtgg cgcgagttaa tggtgtcgag catcgaataa cagacacccg
     4021 ctttcagctt accgtgcaga cctttatcgc gcgaaccgct ggtggccccg gcttccgtag
     4081 tgttacggaa atgcagcagg gtcaggtcgg ggatcagcgc cgtaacgaac gccgccatcg
     4141 tggtggattt acccgccccg ttaccgccgg aaagcgtcgt gaccagctcg tcaaggtcaa
     4201 aagttcgggc aaaaaagccg ttccagttaa tcagcgtcag tgagcgaaat ttaccgcgtt
     4261 caatcattat tcttcctctc cgctatctgg ctgactctct tcggtttcat cgttgagttg
     4321 cagatgattt tcaatcggca ttgcttcgcc atcacgaatc aggcgacgct gtgcttcacg
     4381 gggatcgtcg ccagcacgca catcggctcc gaagcggaac accgattcgg taatgcggaa
     4441 cttgctgctg tcgtggccca taaaccacac catgcctaaa cgacgcagac ggttgagcga
     4501 agaacgtact ttctcctgca acttctgacg gtcaacgtct gaaccggttg aacggttgtt
     4561 caccagtttc agcagtttcg cttcatcggc cagggtgagc agttcgtcgt acagttcctg
     4621 ctgggtgaaa atcccctcat tcgccagccg ttccgggctg agatagagat aacagaggat
     4681 tttcccgacc atcatatcca gttccgacaa gacggaacgc gggataagag tggtggaacg
     4741 tgggcgtaaa tagaagaacc cttccggtgc gcgaataagc tcaacgttat aacgcgcgta
     4801 aaactcttcc agatattcct gaaaatccat caggaatgca tgattatcca gttcgtcgag
     4861 gccaatatgg cggcctgaac gtaaggcgct gtccagcgcc ggaaataacg gattcgccag
     4921 cgcctgcgcc agcttaaccg gcatcacttg ttcaatattt gtcgatgaca tgcgcctgta
     4981 ccttggctcc gtaatcatta atcggctgcc atttcgctgg cagtccggtg aaatctgctt
     5041 gcgctacgcc aagacgtacc gcctgatcaa taacaatacg cgcaacgtca aagtgacgtg
     5101 cacgcggata ctgtgacaga tattcgcgta ccaccagacc aagatccagc ggcacttgtc
     5161 tggttttgta cacggcaagt tgttcttcga tgatcgccgc cagctgttcg cggatctcgt
     5221 taaactcttc gtattccaga tcctcaggaa gttccccagt cacttcttca tcgcgcagtg
     5281 ccatctcttc gtcacgcata tccagcagac gatcggcatt ggcataagtt agcgcccacg
     5341 gctcatcaaa ataggtttgt accgactgac gtaaccgctg agcaaagacg cggtttttat
     5401 ccatatcgat cgcggtacga ataaatttgt gtacgtggcg gtcgtagcca atccacaagt
     5461 cgatggattg ctggccccaa ctgataatac gatcgagttt gctctgcaga tcgaacacca
     5521 gacgatcgac gaaatgcaga tcgtcatggg tcatcgtcgc atcctgaatg cgcaacagat
     5581 tagcctgcaa tttgtcgcct gccgcttcca gcgtatcctg caattcacgc agcgttccgg
     5641 aagtttccga aagcaacaat tcacagctgg aaatcgccgc ccgccagtct ttgttcagca
     5701 actgggcgat atcgtccttc acctgctgct gctgttcgtc catcagacgt tgcgtcaggt
     5761 cgatactgtc gaaaatttct gctaccgaat atttcagtgg cgcatagaca ttacggtgcc
     5821 agtgaaattc atcaccgccc tcttcggcgg catctgctgc gcgtttgagc tcacccgcca
     5881 caatcgacaa ctgcatagaa agacgcagcg tagaaaactc gcgctgacgg atgtagtagt
     5941 cagtaatgcc gatgccgagc ggcgtcagac ggtaaattgc gttcccttcc gcctgctcgc
     6001 tggtaaagcg gttcagcaga cgttgacgca ccatgtcgtt gatcgcgtta ttggcgcgca
     6061 cgccgatggt ttcgctggtt tgctcaaacg catcactcac atggcggaat gcatccacca
     6121 gctcgccttc actcatctca ccatccagac gctcgccgtt cagcgtggca accgccagca
     6181 gaaaagagag tcggtctacc ggcagcgaga tggagaagtc attttttctg gcccaggcaa
     6241 ccagttcggg gactgtctgg gaaaattcac tcatacttta tccttgctct gcggtttgcg
     6301 cgcggtgaca tgaatataac gccctagggt aatatacggt tcctgacggc aataacgcgt
     6361 ttctaattca agtaatgctt catagcagtc gcgctgctgg tacttctcgc gcagataatc
     6421 atgaaacacg cgaacgcctg tcttacccat aatttgccaa ccagcttctt ccagccacag
     6481 ataaacctgc gccgggtcgc gtggataatc tggcgaaagc gtccgttttt tctttttcgg
     6541 cattcccgcc tgcacgtaat caaaattccc ggcgaccatg ttatgcatca acaaaccatg
     6601 cgcattgtag aacattaacg acaacactcc gcctggacgc aacactgacc acagggtctg
     6661 caatacactg cggggatcgg ccacccactc gagcaccgca tggaacaata tcagatcaac
     6721 gggcgtttcc aaatgcgaag caacatcctg agcggcgcaa tgtataaatt gcatgttgtc
     6781 gctcacacct tttgcttctg ccgcctgttt tgcgcggtcg atcatctgcg cagaaagatc
     6841 gcataaaata acctgatgcc cgcgctcggc cattttgatt gcggtctgcc cttctccgcc
     6901 gccagcatcc agcacacgca gtttttgcgg gcccatttcc gccagcacgc gatcgagatc
     6961 ctgccacaga atagcctgtc gaagctgccc tttggtggtg ccgtaaatgt tacgggaaaa
     7021 cttttccgca atatcatcaa aattgcgatc ctgcattgct ttctccaccg gttggctaag
     7081 acgttttctg ataaagacgc tattttgtca cagccacggc gagaatgaac ccgtctggtg
     7141 taatatcgct agcgatccct tgctatatgg ttaaaaaagg aaccagaaag gatgcttttt
     7201 acactgaaaa aagtgattgg caatatgttg ctgccacttc cgctgatgtt gttaattatt
     7261 ggcgctggcc tggcgctgct gtggtttagc cgttttcaaa aaaccgggaa gatttttatt
     7321 agcattgggt ggctggcact cttgttattg agtttgcaac cggtagccga ccgcctgctg
     7381 cgtcccatcg aaagcaccta tccgacatgg aataattcgc aaaaagtgga ctatatcgtg
     7441 gtgctcggag gtggttatac ctggaacccg cagtgggcac cgagctctaa tttaatcaat
     7501 aacagtctgc cacgcctgaa tgagggtatc cgtttatggc gcgaaaatcc gggttcaaaa
     7561 ctgatcttca cgggaggcgt agcaaaaacc aatacggtga gtacagcgga agtaggtgcc
     7621 agagttgcgc aatcgctggg cgtaccgcgc gagcaaatta tcaccctgga tttgccaaaa
     7681 gataccgaag aagaagctgc ggcagtgaaa caggcgattg gtgacgcccc tttcctgctg
     7741 gtgacctccg catcacatct gccgcgggcg atgatctttt ttcagcagga agggttaaat
     7801 ccgctaccag ccccggcgaa tcagttggcc atcgactcgc cgcttaatcc gtgggaacgg
     7861 gcaatccctt ctccggtctg gctgatgcat agcgatcgtg ttggctatga aacgcttggc
     7921 cgcatctggc agtggctgaa aggtccgtca ggcgagccac ggcaagagtg attttgatgc
     7981 cagatcgaag ttgcgccgac taaatcgtcc ggtattaacc agttgcgcaa cttcatccca
     8041 taacacatac aaccaacgcc gccagatgaa cgactccgcc actggcgcgc gttgcaggta
     8101 actccagagc aaatcttccg ccagagaatt atccattagt cggaacagtt cgtattctcg
     8161 cggtgcccaa agcattagtc ccggcccgac catcgccagt aactgatcgc tgcgcgaatc
     8221 tttcagcatg ctgcgtaaac agaagttacc gtgaatcaac acacaattgt cgttaaagcc
     8281 ttcaaataat gccgggagac attcgcgagt gcgaaacagg atccgcttat cctgcatcgt
     8341 cagaccggtg ttattgaact gattgagcgt ggtccatagc acttcaacat gttgccggta
     8401 ccatgagggc cagaaatttt cctgagtatt gtcgaccgcg ccgacgcaac cgcgactgtc
     8461 ctgacggtgc caggccagta aggcttcaac gatttggtct ttgagctgtt cccagcgttc
     8521 tggtgttcgg gctggtgcct ccaccgaaac accacgcatc cgctccagta gcaggacatc
     8581 agggccgggg tgttcttcat gcgtcatcac accataaata gtcggcatac ggacagtccc
     8641 acttcttgcc agcatggtgg ttttccatgc cagttgtcgg gcttttccgg gcgtactaaa
     8701 gcttcttgcc attaacggca ttgggtttcc ctggctgtca tacaaagccc acaacgccgt
     8761 atccgctttt tcattgacgc actcaatacg actgagtttt tcgcccagta aatggcttaa
     8821 ttcggcacgc agctgttcca ttcagattac cctcataaaa gctactgctt tcataatgag
     8881 cgccaatata aaagatgtca cctgaatgag atcaattgtt gaaaagaaaa attgaggaaa
     8941 atcgtaacgg tacgacactc ctcccaaaat tggctgaagt gtcgtgaagt gaaattagcg
     9001 catttcagcg cgaacgcgct caagatcttc aggggtatcc acacctgtgc caggaacttc
     9061 ctgagcaaca gcaacatgga ttttttcgcc gtaccacaga acacgaagct gctctaacat
     9121 ttcgatgtgt tctaacggac ttgcctgcca gttgacgtaa cgacggataa agcctgcacg
     9181 gtagccataa ataccaagat gacgcaggaa gttatcgcca acggtttcaa ggccttctgc
     9241 aaaacgatca cgatcccaag gaatggtggc acgtgagaag tacagtgcat acccttcagc
     9301 gtcgagaacc actttcaccg cattcgggtt aaacgcttct tccgcattgt ggattggcac
     9361 cgccagagtc gccataccca cctgacgctg agcgaggtta tcagcaacct gacgaatgat
     9421 tgtcgcaggg atcatcggtt catcaccctg cacattaacg atcaccgtgt cgtcgctgaa
     9481 tgcgcatttt tcgacaactt ccgccagacg ttctgttcct gactgatgat cggcgcgcgt
     9541 catacatact tcaccgccag cggcttcaac ggcgcgggca acatcatcat gatcggttgc
     9601 cacgatgatg cgctcggcac ctgattcacg cgcgcgttca agaacatgaa caatcatggg
     9661 tttgccgtta atatcaacca atggtttacc gggcagacgc gtggacgcgt agcgcgcggg
     9721 aataatgacc acaaaactca tgatttactc tcatcagcag tcagcacgcg ggcttccgtt
     9781 tccagcaaca ctggaatgcc atcgcggagc gggaaagcca ggttatccag tttgcaaata
     9841 agctcttgtt tttcctggtt ataccaaagt tttccgttgc aaaccgggca ggcaatgatt
     9901 tcaagcaaac gatgatccat agttcctcca gatggatcgg gttatgaatg cataaatctt
     9961 atcataatca tttatgcgac ggggtctatt tcccagccag tgcggcaatc agtaaagaga
    10021 ccttgcgggc agcgtcctaa tgtcacccgc gttgcctgct gccagctcgc gaaatcagta
    10081 atcgcctgac gtaacccttt ttgcagcatt gtcgttggtt taatgccttc ctgtaaccac
    10141 agagagataa cttcaaggat gcctgtctgg cgatgcattt tggcatccat tcgcccaact
    10201 aattgcccac gatgtaataa tggcagaaca aaatagccat actggcgttt cggcgctggg
    10261 gtatagcact ccagccggta gctaaaatca aaaagctgct ctgcgcgttt gcgatcccag
    10321 acaacaggat caaaaggcga aagtaccgcg ctgtgcgttg cagtgagctt tccggccagc
    10381 gcacgctcga gtagcggcag caaatcagca tgcagccaaa gattgcccaa tttttcaaca
    10441 tgcacagcaa tgatttgctg ctgttcagcc ctcgcttcgc gccacgccgc cagtgccgga
    10501 cgtttcagcc gatagtaatc tgccagccac tgttcgcgga atattcccag gctacgcgca
    10561 ctgttatcca gcatgatgat ttctgcttct gtttgcgaaa cgagatcgcg ctcatcatcc
    10621 cagtcaggca tgacacggtg ggttaaatca taaacgcgct ggaagttgcg ccgttcaatc
    10681 accatcacct ttccggcagt aaataaacct tccagatgcc gtttatgcgg cttccattcc
    10741 caccagccgc ttgcaccttt acgaggatgc tcaaaatcgg ctgaacgtac cggcccttta
    10801 tcatgaatat gctgaattaa ctgtgcaatt tccgcctcat gttcctgcat ccaggcgtct
    10861 ttgtatttcc agcccatttt ttcaggtgcc agcatgcggt ggcgaataag acgaaagtcg
    10921 ctacgcggca taaagcaggc ttcatgcgcc cagtattcca ttaattcgcc acgcgccaga
    10981 gactcatcca gccactgggc aggataattt cccagacgac tgaaaagcac cagatatgga
    11041 ctacgggcaa caatattgat ggtatcgatt tgcagcaagg acatgcggga gatcgttgcc
    11101 ggaatatcct ccaacgacgc tcgacggcgg ggtttgttta acaggccttg tgcggcaagg
    11161 tgaagattac gcgcatcagc aagggagagg tgcggcagcg acattcatga ctccatcaat
    11221 cgaacgctgc cgcggcgtaa ctagttgcca gaagccagcg aggttagttg cgtaagcagt
    11281 ttcgctggtt catcacctga aagctgtgcg tctacaggca aataccacca attttcttct
    11341 gcaaaggccc ggcatttcac cgcatctttt tcagtcatta ccagcgtttg cccggcgctt
    11401 accaacgcac tgacatcctc atggttcaaa gactgatgat cggccagcgg tacacatttt
    11461 tccggttgta cgccacacat cttcagcgtg gcaaaaaagc gcggcggatg cccaatcccc
    11521 gccatcgcca ctacatgttc aagctgagca acgtcacaac gcgtaccggt acgtaaattc
    11581 accgcctgac ccggcagcag atgcatgggg atttcaccgc tgcgagggac accgccgttg
    11641 acgattaccg catcaaccga ctttaagcgc cccgctcgct cacgcattgg ccccgccggc
    11701 aaccaccagc cattgccaaa gcgacgcaca ccatcaataa cgacaatttc cacatcacgc
    11761 gccagacggt aatgctgtaa accgtcgtcg gttacgatga tctgcacatc agggtgttgc
    11821 gccagaatgg cttttaccgc atcagaacga acgggagaaa ccgcaacagg cgcatcagtg
    11881 cgttgataaa tcaacacagg ttcatcaccc gcctgtgctg tggtggtatc tgccgacaat
    11941 aacagcggat aagattcagc cttaccacca tatccccgcg atacgacccc cacgcgaata
    12001 ccgcgctgtt gcaactgttc caccagccag acaacgaccg gggtttttcc gttgccgcct
    12061 gcggtgagat taccaaccac gacaaccggt acgggggcac gccaggcgcg cttcagtttt
    12121 agtttatagc aaagacggat cgcgccactc accaggccat acaaccagga gagtggcagc
    12181 aatagccgcc acaaagggga ttcaccagac cagatttttt cgatcattgg ccaaactgca
    12241 ttttgtgaag ttgcgcgtaa acgccgcggt gctcaagcaa atcgttatgc gtaccgcgtt
    12301 ccacaatgac accatcctcg acgaccacga tttcgtctgc cttttcaatg gtagacaagc
    12361 ggtgggcaat caccagagag gtacggtttt tctgcaactc atccagtgcc gcctgaatcg
    12421 cacgttcgga ttcggtatcc agagccgagg tagcttcatc cagaatcaga atcgggctat
    12481 cacgcagcaa ggctcgagcg atagcaatac gctgacgctg accgccagag agcagcacgc
    12541 cgttttcacc aatcactgta tcgagaccgt tatccatctt attgatgaag tccatggcgt
    12601 aggccatccg cgccgcttct tcaatttgct cacggctgta ctgttcagtc cgtgcgtaag
    12661 caatgttgtt agcaaccgta tcgttaaaca gatggacatt ctgcgacacc agagcaacct
    12721 ggttacgtaa cgacgccagg gtatactcgc gcagatcgtg accatccatc aggatttcgc
    12781 cttcatcaat atcgtaaaaa cgcgtgatca ggctggcgat ggttgattta cctgaaccag
    12841 agcgtccaac cagagcaacc gtcttccctg ccggaatttt caggttgatg ttacgcaatg
    12901 cgggtacgtc acgtcccgga taagtaaagg tgacattgcg gaattccacg tcgccagtcg
    12961 cacgctcgat cacgcgctta ccttcgtctt tctcctgctc actgtccaga atggtaaaca
    13021 gcgtctgaca agccgccata ccgcgctgga actgggcgtt aacgttggtc agcgatttca
    13081 gcggacgcat cagtgcaatc attgaagaga aaacaacggt aatcgtaccg gcagtcaggt
    13141 tatccataac acttgggaag ctcgccgcat acagaacaaa cgccagcgcc aaagaggcga
    13201 tcagctgaat gatcggatca gagatggaag aggctgaaac cattttcatc ccctgaagac
    13261 gcattcggtt actgacttta tcgaagcgtt tcgtttccac ttcctgacca ccgaaaatca
    13321 atacttcttt gtggcccttc agcatttgtt ctgcactggt ggtcacttgc cccatggtgt
    13381 tctgcatgtt tttactgatg ttgcgaaaac gcttcgatac tacgcgaatc gcaatcgaaa
    13441 caatcggtgc cagcacaatc aaaatgatcg acagttgcca actgtaatag aacatcatga
    13501 tgaacaggcc gatgatcgac gcaccttcac gcacaacagt aatcagtgcg ccggaagaag
    13561 aagaagcaac ctgttcggaa tcgtaggtga tacgtgacaa cagcgtaccc gttgactgtt
    13621 tgtcaaagaa tgaaactggc attcccatca tgtgaccaaa caggcggcga cgcatggtca
    13681 ttaccacctt tcctgatacc caggagatac agtagctgga gacatagctg gtgataccac
    13741 gtaaaatcat cagtccgatc accaccagcg gcatccacac cagcacggag cgatctgttt
    13801 taccaaagcc atcatcaaga agtggcttaa ggagcgataa catgaaggta tcgctggctg
    13861 cgttgaggat taacgctacg cccgccacga tcagacccgc tttgaaaggc gcaatggttg
    13921 gccacagtcg gcggaatgtc tgccacgtag agagatcttt gtcgttatgc attcaaaaaa
    13981 ccagcatttg ttgaaatagc cgcatattct acccgttatc cactggcacg ccaaaccact
    14041 gatgatacca acgaggtaaa atttgctctc tgaggctgct aatccgccag ccttgtgctg
    14101 aaaaattgac cgtcacttgc ccctgatgtg gagtatcaag ccattgatat cctttctgtt
    14161 gatagcgatg cttaactttg ttagagggca atcgccatgc gttatagcgc gatgccgatg
    14221 cgagtgccac ttttccattc actcgctgaa ttaatggcaa cgatgatgag gtattactgc
    14281 catggtgagg tacctgaagc aatgttgcct gcacttgctg ccagtaacgg cttagcatct
    14341 tttgttcagc tggggcttca atatcaccgg ttagaagaat gctattcgtc ccgtcatcaa
    14401 ccttaaccac acaggaatgg ttatttccct tatcgttgct accttgtaaa ggccagtgcg
    14461 cgctgaaacg caatccttgc cactgccacg cttcgccacg cacacagggc tgatgatgtt
    14521 cccagtttaa cggacttctg atccataaca tcggccatat atgcaatatt gaatccagcc
    14581 ctccccggtg atccagatgt tcatggctca gaataacgcc ttccggttca agattatgcc
    14641 agtggagcca ggggataatc agttgttgcc cactatcccc ttcaggccag tcctgtgtca
    14701 tagagaatcg ctttgccgtt tctggctatc accattgcca gcccttgccc gacatcaagc
    14761 atgtacagct gccactcgtc aggtcgaggt ttttgccaca gcggccagca catcagcaag
    14821 cctccagcca cacacattgc tggcaacgtt cgccaggcgt ttaatcgcca taccaccagt
    14881 aagaaccatg gggaaaatga tagccattgc caacattcaa caatgttgat ccacccttcc
    14941 ggcaaactct ttaaccccca gaaaagtaaa gccaaagacc ggtcggcaag aaaccataac
    15001 ccctgttcca ggattaacgg cccgctaaaa tgcacaacca tcgcggcgag gatcaacgga
    15061 accgtgataa atgtcaccaa gggaattgca aatagatttg caataaacga ggtcagacta
    15121 atgccatgaa atatgacgat ttgcatgggc ataagcagga gtgtgattcc cagttgcaga
    15181 tggatgaggg aaacaactgc acgcaatacc ggcggcagtt gccactcagg acagggaaac
    15241 cactgatacc aaaaaatcag tgccgcgacc gcagcggcag agagccataa actttgcgag
    15301 agaatggcaa caggatccat cagcaaaatt gccgccagac aacatatcca tacatcccag
    15361 ccactccact gtcgcccact taacttaagc attccccacg tagcaagcgc caccacggta
    15421 cgcaatgcag gaggttgcat tcccgtcaac caggcataaa aagcagcaca gcagattccg
    15481 ccaattaatg gtatttgcca gtggatccag cgcccaggca gaaaaatttg tccactgcga
    15541 atgagtccgg cagccagcaa cgccgcaaaa gcgatgtgca atcccgatat cgccattaaa
    15601 tgcgccgttc cagtatcgcg catgatattt ttgatttctt tggggacgga taaccgttcc
    15661 cccataccta aaccaagaat aaccgcattc cacggatagg gttgcagcgt tgtttgtagt
    15721 gacgccagat actgtgcacg caggctacaa ttcggttcaa tgacacttgc ctgcagaaaa
    15781 cggccggtga gcggctgatg ctgggcaatg gcataacgct ggctatcaaa gccgccatca
    15841 ttaagttggc catgaactgc acgaactttg agtttcattg accattgttg tccagcacaa
    15901 accgcttgcg gaagttattc gccatacagc acaaggcctg gcgcagggaa tatacgtcga
    15961 ccttgtagat aagtaatttg accgtaatga gtggtcatgc cgtcagttgc agtgatctca
    16021 acaattgcat cctgcgtcgc gccagttaag gtttctcctg cccacaaaat ttgctttgct
    16081 gataatatgc cccaaacgaa aaacagcaac gtcagcgcga tataacggac ggttttaaca
    16141 gggataaatg ccagtacgca ggcgaagaga gtcagaaacg caagggttaa tgtcctaggc
    16201 aattggggca aaatcagcaa cggaaaaatt ccgcaaatta tgcatacgcc gactgtcgtt
    16261 attttcatgc acccctccct gtttatggaa agtgtgcaac tttgttgagc ggttgtctgg
    16321 tgggaatttt gcgttccccg aggcacattc agaagaattt aacgaaaggg tgaaaactgc
    16381 tcaaaactct gcgaggcagc ttccagttcc tgattagaga attgaaaaaa gcacccgaca
    16441 ggtgcttttc tcgcgttcaa gtttgagtaa aaacttaacc gtaaatattg gcgcgatcgc
    16501 gcagttcttt accaggttta aagtgaggaa cgtattttcc ttccagttct actttatcgc
    16561 cagtcttcgg attacgtccg gtacgtggtg cgcggtagtg caaagagaaa ctgccgaaac
    16621 cgcggatttc aatacgctcg ccctgcgcaa gagtcgaggc catatgctcc agcatctctt
    16681 ttactgcatc ttcaaccgtc ttggcgggaa tgtgcgattg ctgggtggca agtctttcta
    16741 tcaattctga cttggtcatg attcctccgg ttccttaaag gcaaattaag cggctgctgt
    16801 tgctgcattg attaagggcg gccgtagccg cccttagtgc ttgattacag gacgaaacct
    16861 gcaatctgtc aagtaaactc aacaaacttc ggaataaaaa tcccgaagag tcagagaatt
    16921 actcgccttt agctgctttg aaagcttcag ccattgcgtt gttggagaag tttgcatctt
    16981 cctgtttgtt aacagttgcg attgcatctt tctcgtcagc ttcgtctttc gcacgaacag
    17041 acaggctgat tgcgcggttt ttacgatcaa cgccggtgaa tttagcttca acttcgtcgc
    17101 caacgctcag aaccagggta gcgtcttcaa cgcggtcacg ggatgcttca gaagcacgca
    17161 ggtaaccttc aacgccgtca gccagttcta cggttgcgcc tttagcgtca actgcagtta
    17221 ctttaccggt tacgatagcg cctttcttgt tcagagcaac ccagttgttg aacggatctt
    17281 ctgcgagctg tttaacgccc agggagatac gttcacgttc tgcgtcaacc tgcagaacaa
    17341 ctgcagcgat ttcgtcgcct tttttgtatt cacgaactgc ttcttcgcct gcaacgttcc
    17401 aggagatgtc agacaggtga accaggccgt cgatgccgcc gtccaggccg atgaagatac
    17461 cgaagtcagt gatagacttg attttacctt caacacggtc gcccttgttg tgggtttccg
    17521 cgaactgctg ccacgggtta gctttgcact gtttcagacc cagggagata cgacgacgtt
    17581 cttcgtcgat atccagaacc ataacttcca ctacatcgcc aacgttaaca actttggacg
    17641 ggtggatgtt tttgttggtc caatccattt cggaaacgtg taccagacct tcaacgcctt
    17701 cttcgatttc aacgaagcag ccgtagtcag tcaggttggt cacgcgacca gtcagtttgg
    17761 taccttccgg ataacgttta gcgatagcta cccacggatc ttcgcccagc tgtttcaggc
    17821 ccagggatac acgggtacgt tcgcggtcga acttcagcac tttaacagtg atttcgtcgc
    17881 ccacgttgac gatttcgctc ggatgcttaa cgcgtttcca ggccatgtca gtgatgtgca
    17941 gcaggccgtc aacgccgccc agatcaacga atgcaccgta gtcagtgagg ttcttaacga
    18001 tacctttaac ttccatgcct tcctgcaggt tttccagcag ctgatcgcgc tctgcgctgt
    18061 tttcggattc gataacggca cgacgagaaa caacaacgtt gttgcgcttc tgatccagct
    18121 tgattacttt aaattcaagc tctttgcctt ccaggtgcag agtgtcacgc accggacgaa
    18181 cgtctaccag agaacctggc aggaacgcac gaataccgtt cagctcaaca gtgaagccgc
    18241 ccttaacttt gccgttgata acaccggtaa cagtttcagc atcttcgtaa gctttttcca
    18301 gcgtgatcca ggcttcgtga cgtttagctt tctcacggga cagcagagtt tcaccgaagc
    18361 cgtcttctac tgcgtccaga gcaacgtcaa cttcgtcacc tacctggatt tccagctcgc
    18421 cctgggcgtt tttgaactgc tcagccggga tggcggactc agatttcaga ccagcgtcaa
    18481 ccagtactac gtctttgtcg atagcaacaa caacgccacg aacgatagaa cccgggcggg
    18541 tttcgatttc ttttaaggac tcttcaaaga gttgagcaaa agattcagtc atgtttaatc
    18601 ttcaggttta tatttaacgt ccacctggct ccatgccgga tggggttgtt taacataccc
    18661 gctgtcattc cattgcaacg ggggtactgc aaattcggtc gcttatgcga gagccaattt
    18721 ctggcgcgcg tattgtagcg ctttttcaat caattgctca atgcttaagg tggtggaatc
    18781 caacactaaa gcatcggctg ccggaaccag tggcgctacc gctcggttac gatcgcggtc
    18841 gtcgcgttct ttgatctcgg ccaaaaggcg ctcgaagtta acactaaagc ccttctcctg
    18901 caactgtagc atgcggcgat gcgcacgttc ttccgaggag gcgtcaagga aaattttcac
    18961 tggtgcatca gggaatacca ccgttcccat gtcgcggcca tcggcaatca gacctggtaa
    19021 ttcgcgaaac gcgcgttggc gacgcaataa tgcttcacga acgcgtggga atgccgcgac
    19081 ttgtgaagcc gcattcgcca cttcctgagt acgaatttcg ccgctgacat cttccccttc
    19141 gaggatcact tccagattgc cattggtcga cacaaaacgt acatccagat gggatgccag
    19201 cggtaccagc gcatcttccg acgcaacatc aacatggtga tgtaatgccg ccaatgccag
    19261 tacgcgataa attgcacccg agtccagcag atgccattgc aacgcttccg ccatagcctt
    19321 acacaaggtg cctttccctg caccgcttgg gccatcaatg gtaataaccg gggcaattgc
    19381 cgtcatcttt atctccttaa aacaggcgta ccgttaacat aaccgcgcgc attatacgcg
    19441 ccgacgccac aaatcgttaa cgttgtgtgc aaaaaccagt tttgtctgta ttcagatggg
    19501 gatttattgc gcaattatag cgggctggca gaagaccagc ccgaaagatg acaatgattt
    19561 acttaccaga ggcgatacga tcacggatgt gttgcgcacg ctctgttgat ggtgggtgag
    19621 agtcaaacat ggactgggtg cgaccgccat ccaggctagc cagtttttca aagctgccaa
    19681 ccagcccctg ggtgctgatg ccacgtttct tcaacagatc aaaagagaaa tcatctgctt
    19741 cggactcttt atcacgggag tatttagcat taatagcgcc ttctgcgata tcacccaatt
    19801 gtgagcggga aagctgggaa gccacaccgc tggtagctga aatggcatcg cgtgcggcaa
    19861 cgatcgcata agaagctttc atttcagcca gcgagtgacc caacgcgacg tggcccagtt
    19921 catggcccag aacaccttca atttcgttgt cattcatcat gtccatcagg ccactgtaga
    19981 cacgaacaca gccgttcgcc atcgcccatg cgttgacgtc gctggtcata taaaccttat
    20041 agttgaccgg cgtgccatta atattgttac ccaatgcttt ggcgattttt gccagacgtt
    20101 taccgtactt gctggattta cttgcgactt gattgccgct gtccatttcg gcacagccct
    20161 gattggcaat cgcttttgca tcggcatcgc ttaatgttgc tgctttatag gcatttaaac
    20221 cggagctgat agccatattg gtatcaatac cgtgggtatt ttgacaccct gtcagtaatg
    20281 ctgcagaggt cgcaatcgcc agcagtaatt tagtattctt catgtgaatc atccgttgat
    20341 taatcatata taaatattat gtatcaacaa cttacattga caagcgacgc taccattaat
    20401 caaaatgact cacaaggtcc gaaaaaaaa
//
LOCUS       DAJTUI010000072        20294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTUI010000072 DAJTUI010000000
VERSION     DAJTUI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.072"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            161..502
                     /gene="raiA"
                     /locus_tag="OO464_002782"
     CDS             161..502
                     /gene="raiA"
                     /locus_tag="OO464_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6227869.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    605..730
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            606..653
                     /gene="pheL"
                     /locus_tag="OO464_002783"
     CDS             606..653
                     /gene="pheL"
                     /locus_tag="OO464_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR6227870.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            752..1912
                     /gene="pheA"
                     /locus_tag="OO464_002784"
     CDS             752..1912
                     /gene="pheA"
                     /locus_tag="OO464_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6227871.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(1955..3076)
                     /gene="tyrA"
                     /locus_tag="OO464_002785"
     CDS             complement(1955..3076)
                     /gene="tyrA"
                     /locus_tag="OO464_002785"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6227872.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3087..4157)
                     /gene="aroF"
                     /locus_tag="OO464_002786"
     CDS             complement(3087..4157)
                     /gene="aroF"
                     /locus_tag="OO464_002786"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6227873.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4367..4732
                     /locus_tag="OO464_002787"
     CDS             4367..4732
                     /locus_tag="OO464_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6227874.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4882..5400
                     /locus_tag="OO464_002788"
     CDS             4882..5400
                     /locus_tag="OO464_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR6227875.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNVFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5390..6615
                     /gene="dgcN"
                     /locus_tag="OO464_002789"
                     /pseudo
     CDS             5390..6615
                     /gene="dgcN"
                     /locus_tag="OO464_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6631..7113
                     /locus_tag="OO464_002790"
     CDS             6631..7113
                     /locus_tag="OO464_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6227876.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7190..7537)
                     /gene="rplS"
                     /locus_tag="OO464_002791"
     CDS             complement(7190..7537)
                     /gene="rplS"
                     /locus_tag="OO464_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6227877.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7579..8346)
                     /gene="trmD"
                     /locus_tag="OO464_002792"
     CDS             complement(7579..8346)
                     /gene="trmD"
                     /locus_tag="OO464_002792"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6227878.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8377..8925)
                     /gene="rimM"
                     /locus_tag="OO464_002793"
     CDS             complement(8377..8925)
                     /gene="rimM"
                     /locus_tag="OO464_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6227879.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(8944..9192)
                     /gene="rpsP"
                     /locus_tag="OO464_002794"
     CDS             complement(8944..9192)
                     /gene="rpsP"
                     /locus_tag="OO464_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6227880.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9329..10690)
                     /gene="ffh"
                     /locus_tag="OO464_002795"
     CDS             complement(9329..10690)
                     /gene="ffh"
                     /locus_tag="OO464_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6227881.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10857..11648
                     /locus_tag="OO464_002796"
     CDS             10857..11648
                     /locus_tag="OO464_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6227882.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11669..12955
                     /locus_tag="OO464_002797"
     CDS             11669..12955
                     /locus_tag="OO464_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6227883.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13010..13603)
                     /gene="grpE"
                     /locus_tag="OO464_002798"
     CDS             complement(13010..13603)
                     /gene="grpE"
                     /locus_tag="OO464_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6227884.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13726..14604
                     /gene="nadK"
                     /locus_tag="OO464_002799"
     CDS             13726..14604
                     /gene="nadK"
                     /locus_tag="OO464_002799"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6227885.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14690..16351
                     /gene="recN"
                     /locus_tag="OO464_002800"
     CDS             14690..16351
                     /gene="recN"
                     /locus_tag="OO464_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6227886.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16500..16841
                     /gene="bamE"
                     /locus_tag="OO464_002801"
     CDS             16500..16841
                     /gene="bamE"
                     /locus_tag="OO464_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6227887.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16903..17193)
                     /locus_tag="OO464_002802"
     CDS             complement(16903..17193)
                     /locus_tag="OO464_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR6227888.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17183..17620)
                     /gene="ratA"
                     /locus_tag="OO464_002803"
     CDS             complement(17183..17620)
                     /gene="ratA"
                     /locus_tag="OO464_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6227889.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17791..18273
                     /gene="smpB"
                     /locus_tag="OO464_002804"
     CDS             17791..18273
                     /gene="smpB"
                     /locus_tag="OO464_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6227890.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18488..18850
                     /gene="ssrA"
                     /locus_tag="OO464_002805"
     tmRNA           18488..18850
                     /gene="ssrA"
                     /locus_tag="OO464_002805"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19055..>20294
                     /locus_tag="OO464_002806"
     CDS             19055..>20294
                     /locus_tag="OO464_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6227891.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5246 a         5309 c         4760 g         4979 t
ORIGIN      
        1 ttgagatatt cctcaagtaa aaaaacacct cttcctgcga tttctcacaa aaaagattcg
       61 ttgacaaaaa gtgacaaaat tatgagattt ccatcacaca ttttgacatc aggaacggta
      121 tgctgaattc accaagacgg gaagacaaga ggtaaaattt atgacaatga acattaccag
      181 caaacaaatg gaaattactc cggccatccg ccaacatgtc gcagaccgtc tcgccaaact
      241 ggaaaaatgg caaacacatc tgattaatcc acatatcatt ctgtccaaag agccacaagg
      301 gtttgttgct gacgccacaa tcaatacacc taacggcgtt ctggttgcca gtggtaaaca
      361 tgaagatatg tacaccgcaa ttaacgaatt gatcaacaag ctggaacggc agctcaataa
      421 actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg gtgaaagacg ccaacttcgt
      481 cgaagaagtt gaagaagagt agtcctttat attgagtgta tcgccaacgc gccttcgggc
      541 gcgttttttg ttgacagcgt gaaaacagta cgggtactgt actaaagtca cttaaggaaa
      601 caaacatgaa acacataccg tttttcttcg cattcttttt taccttcccc tgaatgggag
      661 gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata aggcctccca aatcgggggg
      721 ccttttttat tgataacaaa aaggcaacac tatgacatcg gaaaacccgt tactggcgct
      781 gcgagagaaa atcagcgcgc tggatgaaaa attattagcg ttactggcag aacggcgcga
      841 actggccgtc gaggtgggaa aagccaaact gctctcgcat cgcccggtac gtgatattga
      901 tcgtgaacgc gatttgctgg aaagattaat tacgctcggt aaagcgcacc atctggacgc
      961 ccattacatt actcgcctgt tccagctcat cattgaagat tccgtattaa ctcagcaggc
     1021 tttgctccaa caacatctca ataaaattaa tccgcactca gcacgcatcg cttttctcgg
     1081 ccccaaaggt tcttattccc atcttgcggc gcgccagtat gctgcccgtc actttgagca
     1141 attcattgaa agtggctgcg ccaaatttgc cgatattttt aatcaggtgg aaaccggcca
     1201 ggccgactat gccgtcgtac cgattgaaaa taccagctcc ggtgccataa acgacgttta
     1261 cgatctgctg caacatacca gcttgtcgat tgttggcgag atgacgttaa ctatcgacca
     1321 ttgtttgttg gtctccggca ctactgattt atccaccatc aatacggtct acagccatcc
     1381 gcagccattc cagcaatgca gcaaattcct taatcgttat ccgcactgga agattgaata
     1441 taccgaaagt acgtctgcgg caatggaaaa ggttgcacag gcaaaatcac cgcatgttgc
     1501 tgcgttggga agcgaagctg gcggcacttt gtacggtttg caggtactgg agcgtattga
     1561 agcaaatcag cgacaaaact tcacccgatt tgtggtgttg gcgcgtaaag ccattaacgt
     1621 gtctgatcag gttccggcga aaaccacgtt gttaatggcg accgggcaac aagccggtgc
     1681 gttggttgaa gcgttgctgg tactgcgcaa ccacaatctg attatgaccc gtctggaatc
     1741 acgcccgatt cacggtaatc catgggaaga gatgttctat ctggatattc aggctaatct
     1801 tgaatcagcg gaaatgcaaa aagcattgaa agagttaggg gaaatcaccc gttcaatgaa
     1861 ggtattgggc tgttacccaa gtgagaacgt agtgcctgtt gatccaacct gatgaaaagg
     1921 tgccggatga tgtgaatcat ccggcactgg attattactg gcgattgtca ttcgcctgac
     1981 gcaataacac gcggctttca ctctgaaaac gctgtgcgta atcgccgaac cagtgctcca
     2041 ccttgcggaa actgtcaata aacgcctgct tatcgccctg ctccagcaac tcaatcgcct
     2101 cgccgaaacg cttatagtaa cgtttgatta acgccagatt acgctctgac gacataatga
     2161 tgtcggcata aagctgcgga tcctgagcaa acagtcgccc gaccatcgcc agctcaaggc
     2221 ggtaaatcgg cgaagagagc gccagaagtt gctcaagctg aacattttct tctgccaggt
     2281 gcagcccgta agcaaaagta gcaaagtggc gcagagcctg aataaacgcc atattctgat
     2341 cgtgctcgac agcgctaata cgatgcaacc gagcgcccca gacctgaatt tgctccagaa
     2401 accattggta tgcttccggc ttacgtccat cacaccagac cacaacttgc tttgccaggc
     2461 taccgctgtc cgggccgaac atcgggtgta accccagtac cgggccatcg tgcgccgcca
     2521 gcatggcctg taatggtcca tttttcactg atgccagatc aaccagaata caatctttcg
     2581 gtaaaggcgg taatttgccg ataacttgct cagtaacgtg gattggcaca ctaacaatca
     2641 ccattccggc atcggcaaca atatcagccg ctcgatccca gtcatgttgc tccagaatcc
     2701 gcacctgata acccgatagt gtcagcatct tctcgaacag gcgtcccatc tgaccgccac
     2761 cgccgacgat aaccaccgga cgcagtgacg gacaaagtgt tttaaatcct ttgtcgtttt
     2821 cactggagta agattcacgc atcacccgac gcaaaacatc ctcaatcaga tctggcggta
     2881 cacccagagc ttccgcctct gcacgacgcg aggccaacat agatgcctcg cgctccggaa
     2941 cataaatagg cagtccaaag cggcttttca ccttgcccac ttcagcaacc agttccagac
     3001 gcttcgctaa taaattcagc agcgctttat cgacttcatc aatttgatcg cgtaatgcgg
     3061 tcaattcagc aaccataata aacctcttaa gccacgcgag ccgtcagctg cccgttcaga
     3121 tcctgatgaa tttcacgcag caaggcatcg gtcatttccc agctaatgca ggcatcggtt
     3181 acggatacac cgtatttcat ttcactgcgc ggttgctcag aagactgatt gccctcgtgg
     3241 atattacttt cgatcatcag accaataatt gagcgattac catctttgat ttgagcaacc
     3301 acggattctg ccaccgcagg ctgacggcga taatctttat tggaattacc gtggctgcaa
     3361 tctaccatca gagacgggcg cagtcccgcc tgttccatct ctttttcaca ttgcgcaaca
     3421 tccgcagggc tatagttcgg cgctttacca ccgcgcagga tcacatgccc gtccggattc
     3481 ccctgagttt gtagcaacgc aacctgccct gcctggttaa tgccaacaaa acagtgcggc
     3541 tgggcggcgg cgcgcatagc gttaattgct gttgccagac tgccgtcggt gccgttttta
     3601 aaaccaaccg gcatggaaag cccggaggcc atttcacggt gagtttgcga ttccgttgta
     3661 cgagcaccaa ttgctgacca gctaaacaga tcgcccaggt attgcgggct attcggatct
     3721 aacgcttccg tcgccagtgg cagtcccata ttcaccagct caagcaacaa tttacgcgcg
     3781 atttgcagcc cggcttctac atcaaaagag ccatccatat ggggatcgtt aattaaccct
     3841 ttccagccga cagtggtacg gggtttttca aaatagacgc gcattaccag atagaggcta
     3901 tcgctgacct ctgcggcaag agctttaaat cgacgagcat attccagagc agtttccgga
     3961 tcatgaatgg aacaaggacc acatactacc agcagacgag gatcgcgccc ggcgataata
     4021 tctgaaatgg ttttacgcga gtcagcaatc tgggcttctt gttgcaggct caatggaaaa
     4081 gcggccttca gttgttccgg agtcattaaa acctgttcgt cggtaatatg tacgttattc
     4141 agcgcgtctt tatgcatgat ggcgatcctg tttatgctcg tttgcgatag ttgctcctca
     4201 gcgaggatga cgtaacgata acacataaag taaagttttc aacccatatt tcgtacattt
     4261 ttatttacac aggcaattta gtcgcgcttt caacccttgc ttctgtatag ataaatttac
     4321 atccccttta aaaacaatcc gctatgcttt gaaaaaggag aaagaaatga tgaaaaagtt
     4381 tatcgccccc ttgttggctt tactggttag cggatgtcag attgatcctt atactcacgc
     4441 gccaaccttg accagcaccg actggtatga tgtcggtatg gaagatgcga tatcgggcag
     4501 cgccataaaa gatgacgatg catttagcga ttcacaggcg gatcgcggtc tataccttaa
     4561 aggatatgcc gaagggcaaa agaaaacttg ccagaccgat tttacttatg cccggggact
     4621 ttccggtaaa agctttcctg ccagctgcaa caatgttgaa aatgccagcc aactgcatga
     4681 agtatggcaa aaaagggctg atgaaaatgc cagcacaata cgattaaatt aattaaaacc
     4741 ccacaaataa attaaacata agattttact cattgtgttt tatttctcac attgatgacg
     4801 gtcgcatgaa aaaatgatat tttaatgagc atcctgaaaa aatatcgatg catttcgagc
     4861 gaagatggtg gggatccctg aatgcgtttt tctcaccgac tgttcttact tctgattctc
     4921 ctgctgacgg gagcaccaat actcgctcaa gagccttctg acgttgcgaa aaacgtgcgt
     4981 atgatggttt ccggtattgt cagttacacc cgctggcctg cgctatcagg gccaccaaag
     5041 ttatgtatct tttcatcttc acgttttagt accgccctac aagagaatgc cgcgacatct
     5101 ttgccttatc tgcctgtcat cattcacact caacaagagg cgatgatttc aggctgcaat
     5161 gttttttatt ttggcaacga gtcaccgaca ttccagatgg aattaaccga acaatatccg
     5221 tcaaaggcgt tattattaat tgccgaacaa aataccgagt gcattattgg tagcgccttt
     5281 tgtctgatca tccacaataa tgacgtcaga tttgccgtaa acctggatgc ctcatcgcgt
     5341 agcggtgtaa aggtcaaccc ggatgtctta atgctcgcac ggaagaaaaa tgatggataa
     5401 cgataattct cttaataagc gccccacgtt taaaagagca ttacgcaaca tcagtatgac
     5461 cagcatattt atcactatga tgctgatctg gttgctgctt tccgtgacct cggtgctgac
     5521 cctgaaacag tacgcgcaaa aaaacctggc actgacagca gcaacaatga cttacagtct
     5581 ggaagcagct gtcgtttttg ccgatggccc tgcagcaact gaaacactgg cagcgctggg
     5641 ccagcaaggg cattttcaac tgcagaagta cgtgataagc agcaaaatat tctggcatcc
     5701 tggcattacc cccgtaagga tccaggcgat actttcagca atttcataag ccactggctc
     5761 ttccctgccc ccatcattca gccgattcgt cacaatggtg aaaccattgg cgaagtacgc
     5821 ttaaccgctc gcgacagttc aatcagccat tttatctggt tttcgctcgc cgtactgacc
     5881 ggttgtattc tgctggcatc aggcatcgca attaccctca cccgccattt gcacaatggc
     5941 ctggtggaag cactgaaaaa tatcaccgat gtcgtacatg atgtgcgttc caaccgcaat
     6001 ttttcccgac gagtttcgga agaacgtatc gctgagtttc accgcttcgc tctcgacttc
     6061 aacagtctgc tggatgaaat ggaagagtgg cagcttcgtt tacaggccaa aaatgcgcag
     6121 cttctgcgta ccgcgctaca tgacccatta accgggctgg ctaaccgcgc agcgtttcgt
     6181 agcggcatca atacgttgat gaacaattcc gatgcccgaa aaacgtcggc gttactattt
     6241 cttgatggcg ataatttcaa atatatcaat gatacctggg gtcatgcgac gggcgatagg
     6301 gtcttgattg aaatcgcaaa acggttagct gaatttggcg ggctgcgaca taaagcatac
     6361 cgcctgggcg gcgatgaatt cgctatggtg ctctatgatg tacagtcgga atctgaagtg
     6421 cagcagatat gctcagcact gacacaaatc tttaatctcc cgtttgatct tcataatggc
     6481 catcagacca ccatgacatt aagcattggt tacgcgatga ccattgagca cgcctctgcg
     6541 gaaaaattac aagagcttgc cgatcacaat atgtatcagg ccaaacacca gcgtgccgaa
     6601 aagctggtga gataacaagg atatatcgat atgataaagc acctggtagc acccctgatt
     6661 ttcacctcac taatactgac tggctgccag tcccctcagg gaaagtttac tcctgagcaa
     6721 gtcgccgcta tgcaatctta tggatttact gaatccgccg gcgactggtc gctgggctta
     6781 tcagatgcca ttctgttcgc aaaaaatgac tacaaattgc tcccggaaag ccagcaacag
     6841 atccaaacca tggcagccaa attggcctcg acagggctaa cacatgcccg tatggatgga
     6901 cacaccgata actatggtga agacagttac aacgaaggct tatcattgaa acgggcgaat
     6961 gtcgtggccg atgcatgggc tattggtgga caaattccac gcagcaatct caccacacag
     7021 ggtttaggaa aaaaatatcc catagccagt aacaagaccg cccagggccg cgccgagaac
     7081 cgccgcgtcg cagtggtgat tgcaacccct taaaacacga gcaaacagac aaaaaaaggg
     7141 ccagccaatt ggccagccct tcttaacagg atgtcgctta agcgaaatct tagttaagac
     7201 gctctttgat acgagcagcc ttaccagtac gctcacgcag gtagtacagt ttagctttac
     7261 gaacagcacc acgacgtttg acagaaatgc tgtcaactac cggagagtga gtctggaaga
     7321 cacgctcaac gccttcgccg ttggaaattt tacgaacagt gaatgcagag tgcagaccgc
     7381 ggttacgaat agcgataacc acgccctcga atgcctgcag acgttttttg gaaccttcaa
     7441 caacccatac tttcacttcc acggtatcac ccggacggaa ggaaggtacg tcctgcttca
     7501 tctgctcttg ttcaagttgc ttaataatgt tgctcataat ttaatctctt atcctgggta
     7561 aactgatctc tcgggggctt acgccatccc atcatgttta tgttgctgtt gtgcgtgttc
     7621 cgttttgaac tccgccagca accttgcttg ctcttcagtc agagccaggt tttccagaag
     7681 ttcaggtctt ctaagccagg tacggcccag cgactgtttc aaacgccagc gacgtatctc
     7741 ggcatggttg cccgacagta acactggcgg aacttccatc ccttctaaca cctcaggccg
     7801 cgtatagtgc gggcaatcca gcaatccttc agcaaaggaa tcttccgttg ccgaggcttc
     7861 atgtcccagt actcccggaa taaaccggga aacggagtca atcagcgtca ttgctggtaa
     7921 ctcaccacca ctgagaacgt aatcgccgat tgaccattct tcgtcaattt cggtttggat
     7981 cacgcgctca tctatacctt cgtagcgacc gcacaccaga atcaattttt gattcgttgc
     8041 cagttcgctg acgcccgctt gatcaagctt gcgtccctgt ggtgacagat aaatcacctt
     8101 tgcgccttca cccgccgcgg cttttgctgc atgaatggcg tcccgcaagg gttgcaccat
     8161 cattaacatc cccggtccgc cgccgtaagg acgatcgtcc acggtacggt gccggtcatg
     8221 cgtgaagtcg cgaggactcc aactctggat gctcagcagg ccatttttaa ctgcccggcc
     8281 agttaccccg taatcggtaa ttgcgcggaa catttcagga aacaggctaa ttatgccaat
     8341 ccacatagcg ccgcctttta ccgtttatcc ggtggtttaa aaaccaggat cccaatctac
     8401 ttcgattgaa cgagtagtga gatcgacttt cttgataacc tgcccatcga ggaacggtac
     8461 gagacgttcc ttgataccaa acgcatcttt caggtttgcc ttaatgacga gaacgtcatt
     8521 agatccggtt tccatcatat caacgacttt accgagatcg tagccttcag tggttactac
     8581 ctggcagccc atcaggtctt tccagtagta gtccccctct tcaagctgag gcagctgcga
     8641 tgaatccacg acaatttcac aattcgtcag caggttcgcc gcatcacgat cgtcaacgcc
     8701 tttcagcttg atgatcatgt cctgattgtg gtgcttccag ctttccagct ggacttgctg
     8761 ccactgaccc gccttctgga taaaccaggg ctgatagtca aaaatgcttt cggcgtcttc
     8821 ggtggaagaa aacactctga gccacccacg aataccgtaa gacgaaccca tttttcccaa
     8881 aacgatggga tcaacaggtg cttgcgcggt gagttgtttg ctcatcatga ccaccgtgac
     8941 agattaagct gctttgttta cttctttgat cagcgcagca acgcgatcag aaatagttgc
     9001 gccctggcca acccagtgag cgatgcgatc cagatccagg cgagtgcctt cttctttttc
     9061 gctagcgatt gggttgaaga aaccaacgcg ctcgatgaag cgaccgttgc gtgcattacg
     9121 gctgtcagcg acaacaacct ggtagaacgg acgcttttta gcgccgtgac gtgctaaacg
     9181 aatagttacc ataacatcct cttgtgtgaa taaaacaacc ggaccccatc gaggaacgga
     9241 gtccggtgtc atattaaaag cccgaaaatt ttactgattt ctggagaaaa tgcaatcagc
     9301 agttaataac tctgtcgcat aaggtcattt agcgaccagg aaatcccggc ggcatcattc
     9361 ccttcatgct tctcatcatc ttcgccattc cgcctttctt cattttcttc atcatgcgct
     9421 gcatgtcgtc gaactgcttc agaaggcggt taacgtcctg tacctgcatc ccgcaaccgg
     9481 cggcgatacg acgcttacgc gaacctttga tgatttctgg cttagcgcgc tctttcatcg
     9541 tcattgagtt gatgatggct tccatacgca ccagcacttt atcgtccatc tgcgacttga
     9601 cgttatccgg gatctgcccc atgcccggca gtttgcccat caagctggcc atgccgccca
     9661 tatttttcat ctggcgcagt tgctcaagaa agtcgttgag atcgaagccg tcgccttttt
     9721 ttagcttgct ggctaatttc tctgcctgcg cgcggtcaac tttgctttcg atatcttcga
     9781 tcagcgacag tacgtcgccc atgccgagaa tacgcgacgc gatgcggtcc ggatggaacg
     9841 gctccagcgc ctcagttttc tcgccaacac cgaggaactt gatcggtttg ccagtgatgt
     9901 gacgaataga gagcgccgca ccgccgcggg catcgccgtc cactttggtc aacactacgc
     9961 cggtaagcgg taacgcttca ttgaatgctt ttgccgtatt ggccgcatcc tgaccggtca
    10021 tggcgtcaac cacaaacagg gtttcaaccg ggttaatcga cgcatggact tgtttgatct
    10081 cgtccatcat cgcttcgtca acgtgcagac gaccagcggt atccaccagc agcacgtcgt
    10141 agaatttcag tttggcttct ttcagcgccg cgttaacaat atcaaccggc ttctgaccaa
    10201 catcagaagg gaagaaatca acgcccacct gctctgccag cgtctcaagc tgtttgattg
    10261 ccgccgggcg atagacgtca gcagaaacca ccagcacttt cttcttgtgc ttctcgcgca
    10321 ggaaattacc gagtttacca acgctggttg ttttaccggc accttgcagg cctgccatca
    10381 gcacgaccgc aggcggttgc gcagccagat tcagggtctg gttctcttcg cccatcgccg
    10441 caaccagttc gttacggacg attttgacga actcctgccc cggcgtcagg ctcttattaa
    10501 cttcatgacc aaccgctttc tcttttacgc gattgataaa ctcacgcact accggcagag
    10561 cgacgtccgc ctccagcagc gccatgcgca cttcgcgcag cgtatctttt acgttgtctt
    10621 cagtgaggcg tccacggcca ctgatattgc gcagcgtgcg cgacaaacga tcggttaaat
    10681 tatcaaacat tgtctctcgc ctggggtgga aacggttggc cgcaatcgcg acacatcatc
    10741 agtattttgc cgcagtataa catgaaggcg tctttgttgt tatgcaacgg ttggagcagc
    10801 gttcacctga cgctatactg cttctctttc ttattgctca aactgtcgac attactatgc
    10861 ccgtttttgc tctgctcgcg cttgtcgcct actccgtcag tcttgcgctg attgttcccg
    10921 gtctgctgca aaaaaacggc ggctggcggc gcatggctat tatttctgcg gtcattgcgc
    10981 tggtctgcca cgcaatcgct ctggaagccc gcatcctgcc cgacggtgat agcggacaaa
    11041 acctcagcct gctgaacgtt ggttcattgg tcagtttggt gatctgtacg gtaatgacca
    11101 ttgtggcttc tcgcaatcgt ggctggttgc tgctacccat tgtctatgcc tttgcgctta
    11161 tcaacctggc gctggcgacc ttcatgccca atgaatacat cacccatctg gaagcaacgc
    11221 ctgggatgct gatgcacatt ggcttatcgc tcttttccta tgccacgcta attatcgccg
    11281 ccctgtacgc gctgcaactg gcgtggattg attaccaact gaagaacaag aagctggcat
    11341 ttaaccaaga aatgccgcca ttgatgagta tcgagcgtaa aatgttccac atcacgcaga
    11401 ttggcgtggt gctgctaacg ctcacgcttt gcactggcct gttctacatg cacaacctat
    11461 ttagcatgga aaatatcgac aaggctgtgc tctctatcgt ggcgtggttt gtctatattg
    11521 tgctgctgtg gggacattac catgaaggat ggcgtggacg ccgcgtcgtc tggtttaacg
    11581 ttgcgggcgc ggtaattctg acactggcct acttcggcag ccgaattgtc cagcagttaa
    11641 tcagctaaac ccagaaaagg agtttcccct ggaacacatt tctactacta cgttgatcat
    11701 tattctgatc atcatggtgg tcatttcagc ctatttttcc gggtccgaaa ccggaatgat
    11761 gaccctcaac cgctatcgtc ttcgacatat ggcgaaacag ggtaatcgct cggccaaacg
    11821 cgtcgaaaaa ttgctgcgta agccagaccg cctgataagc ctggtgttaa tcggcaataa
    11881 cctggtcaat attcttgcct ccgcgctcgg cactattgtc gggatgcgtt tgtacggcga
    11941 tgcgggcgtg gcaattgcga ctggtgtgct gacttttgtc gtactggttt ttgctgaggt
    12001 attgccgaaa accattgccg cgctgtaccc ggaaaaagtc gcttatccga gtagttttct
    12061 gctggctccg ctgcaaattt tgatgatgcc gctggtctgg ttgctgaatg ctatcacccg
    12121 tatgctgatg cgcatgatgg gtatcaaaac cgatatcgtg gttagcggct ctttgagcaa
    12181 agaagagttg cgcactatcg tgcatgaatc gcgctcacaa atttcccgtc gcaatcagga
    12241 tatgctgctg tcggtgctcg atctggaaaa aatgaccgtt gatgacatca tggtaccgcg
    12301 cagtgaaatt atcggtattg atatcaacga tgactggaaa tctattctgc gccaactctc
    12361 ccactcacct cacgggcgca tcgtgcttta ccgtgattcg ctggacgacg ccatcagtat
    12421 gctgcgagta cgtgaagcct ggcggttgat gtcggagaaa aaagagttca ccaaagaaac
    12481 catgctgcgc gccgcggacg agatctattt tgtgcctgaa ggtacgccgc tcagcacgca
    12541 gttggtaaag tttcagcgca acaaaaagaa agtcggcctg gtcgtcaacg agtatggaga
    12601 cattcagggg ctggtgacgg ttgaagatat tctggaagag attgttggcg atttcactac
    12661 gtcgatgtcg ccaacacttg ccgaagaggt cacgccgcaa aacgacggtt cggtgattat
    12721 cgatggcacc gccaacgtgc gggaaatcaa caaagccttt aactggcatc taccggaaga
    12781 tgatgcccgc acggttaatg gcgtcattct tgaggcactg gaagagatcc ctgtcgcagg
    12841 cacccgcgtg cgtattggca agtacgatat cgatattctc gacgtacagg acaatatgat
    12901 taagcaggta aaagtttttc ctgtgaaacc gctgcgcgag agtgtggcgg agtaacgaaa
    12961 acggccctgc attcgcatgc agggccgtga attattacga aaacagaaat tacgcttttg
    13021 ctttcgctac agttaccatc gccgcacgaa tcgtacgacc attcagcgta taacccttct
    13081 gcataatgcc cagtacgtta cctggcgcaa cgtcatcaga ttccaccatt gcgatagcct
    13141 gatgcacatt cgggtccagc ggcacgttag tttcggcgat cacttcaaca ccaaacttac
    13201 gcacaacatc cagcatcgac ttcagcgtca gctcaatgcc ttcaaccatc gcagacatat
    13261 ccgggttagc tttatcagcc acttccagcg cacgatccag gctatcaatc accggcagca
    13321 attcgttgat gaatttctcc agcgcgaatt tgtgggcttt ttcaatatcc agttcagtac
    13381 gacgacgcag gttttccatt tcggctttta cgcgcaaaat accgtcacgt tcacgggtct
    13441 gggcttcagc cagctgagct tcgagattcg caattttttc atcgcgcgga tccacctgct
    13501 cagcagaagc ttctggctca actgcctcaa tctcttcgtg ctgatccatg ataatttctt
    13561 ccggggcttg cccctcaggc gttttctgtt ctttactact catgaatttc tccgcgtttt
    13621 tttcgcattc atctcgctaa cttcgcttat tatggggatc agtttcaggg tttcaaggga
    13681 agcactcaca ttgtcatcaa tcttcgcaac aaggacctcg gaaaaatgaa taatcatttc
    13741 aagtgtattg gcattgtggg acacccacgg caccctactg cactgacaac acatgaaatg
    13801 ctctaccgct ggctgtgcac aaaaggttac gaggtcatcg ttgagcaaca aatcgctcac
    13861 gaactgcaac tgaagaatgt gaaaactggc acgctcgcgg agattgggca actggctgat
    13921 ctcgcggtag tcgttggtgg cgacggtaat atgctgggcg cggcgcgcac gctcgcccgt
    13981 tacgatatta aagttattgg aatcaaccgt ggcaacctgg gtttcctgac tgaccttgac
    14041 cccgataacg cccagcaaca attagccgat gtgctggaag gtcactacat cagcgagaaa
    14101 cgttttttgc tggaagcgca agtctgtcag caagattgcc aaaaacgcat cagcaccgcg
    14161 attaacgaag tggtacttca tcctggcaaa gtggcgcata tgatagagtt cgaagtgtat
    14221 atcgacgaga tctttgcgtt ttctcagcga tctgatgggc taattatttc gacgccaaca
    14281 ggctccaccg cctattccct ctctgcgggc ggtcctattc tgaccccctc tctggatgcg
    14341 attaccctgg tgcccatgtt cccgcatacg ttgtcagcac gaccactggt cataaacagc
    14401 agcagcacga tccgtctgcg tttttcgcat cgccgtaacg acctggaaat cagttgcgac
    14461 agccagatag cactgccgat tcaggaaggt gaagatgtcc tgattcgtcg ctgtgattac
    14521 catctgaatc tgattcatcc gaaagattac agttatttca atacattaag caccaagctc
    14581 ggctggtcaa aaaaattatt ctaattttac gccagcctct ttactgtata taaaaccagt
    14641 ttatactgta cacaataaca gtaatggttt ttcatacagg aaaacgacta tgttggcaca
    14701 actgaccatc agcaactttg ctatcgttcg tgagcttgag attgattttc atagcggcat
    14761 gaccgtaata actggcgaga ccggtgcggg taaatctatt gcaatagatg ccctcggtct
    14821 ttgtctcggt ggtcgcgctg aagccgacat ggtgcgtacc ggcgctgctc gcgctgacct
    14881 gtgcgcccgt ttttctctga aagatacgcc agcggccctg cgctggctgg aagaaaacca
    14941 gcttgaagac gggcatgaat gtttgcttcg tcgcgtgatc agcagtgatg gtcgctcccg
    15001 tggtttcatc aacggtacag ctgttcctct gtcacaactg cgcgaactgg gtcagttgct
    15061 gattcagatc catggtcagc acgctcatca attactcacc aaacctgagc accaaaaatt
    15121 cctgcttgat ggctatgcca atgaaacctc tctacttcag gaaatgaccg cacgttatca
    15181 gttgtggcat caaagctgcc gtgacctcgc gcatcatcaa cagttaagtc aggaacgcgc
    15241 cgcccgtgcg gaactgctgc aataccaatt aaaagaactt aacgaattta atccgcagcc
    15301 cggagagttt gagcaaatcg acgaagagta caaacgtctg gcgaacagcg gtcaattgct
    15361 gaccaccagc cagaatgcat tggcattaat ggccgacggt gaagacgcaa acctgcaaag
    15421 tcagctttac acggctaaac aactggtgag cgaattgatt ggcatggaca gcaaactgtc
    15481 cggcgtactt gatatgctgg aagaagctac catccagatt gctgaagcca gcgacgaact
    15541 gcgccactac tgcgatcgtc tggatctcga tcccaaccga ctgtttgaac ttgaacagcg
    15601 catctcaaaa cagatttcgc tggcacgtaa acatcacgtc agccctgagg cattaccaca
    15661 gtattaccag tcgctactgg aagaacagca gcaactggac gatcaggccg actcacaaga
    15721 aacgctcgcg ctggcggtaa cgaaacatca tcagcaggca ctggaaaccg cgcgcgcatt
    15781 acaccaacaa cgccagcaat atgcagaaga acttgcacag ctgatcaccg acagtatgca
    15841 tgcgctctca atgccgcatg ggcagtttac gattgatgtc aaatatgacg agcatcacct
    15901 gggcgctgac ggtgccgatc gtattgagtt tcgggtaacc accaacccag gtcagccaat
    15961 gcagcctatt gccaaagtcg catccggtgg tgaattgtcc cgcatcgcac tggcaatcca
    16021 ggtcatcacg gcgcgtaaaa tggaaacccc ggcactgatt tttgatgaag tggatgtagg
    16081 gattagcggt ccaacagcgg cagttgtcgg caaactgctg cgtcaactcg gcgaatcaac
    16141 tcaggtgatg tgtgttaccc acctgccaca agtcgcggga tgtggtcatc aacactattt
    16201 tgtcagcaaa gaaaccgatg gtgcgatgac agaaacacat atgcaatctc tggataaaaa
    16261 agcgcggtta caagagctgg cgcgcctgct tggtggcagt gaagtcacac gtaacacact
    16321 ggcgaatgcg aaagaactgc ttgcagcgta aacttttttc ctgcttcacg gtcagagtaa
    16381 acagcaaaac gccgtaagac cggaaagcaa aaggttttaa agtgatgaaa ggtctattat
    16441 catcggcata ttacagatga gccacgtact gctcgggccc gaaaaggaat caaatcacta
    16501 tgcgctgtaa aacgctgact gctgcagcag cagtactatt gatgttaacc gcaggctgtt
    16561 ccactctgga gcgagtggtt taccgtcctg acatcaacca ggggaactat ctgaccgcta
    16621 acgacgtatc caaaatacgt gttggcatga cgcaacaaca agttgcgtac gcattgggta
    16681 caccgctgat gtccgatcca tttggtacga atacctggtt ctatgtcttc cgccagcaac
    16741 caggtcatga aggtgtaacg cagcaaacgc tgacgctgac ctttaacagt agcggtgtgt
    16801 tgaccaatat tgataacaaa cctgcgctga gtggtaacta ataataaagt tgctctcaaa
    16861 gacgttaaaa aaggtgctca atgagcacct tttttctgtc tgttatttat tcgctgattt
    16921 ttctgctcgt tgcctgcgaa gctctttcgg atcggcaatg agagggcgat aaatctccac
    16981 ccgatcgcca tcatgcacac tatcgcttag ttttgccgga cggctgtaaa tgccgacttt
    17041 gtttttagtt aaatcgatat cggtacgcaa ttccagcaag ccactggcgc gaatagcttc
    17101 ttcaaccgtc gcgccctcct gcagcgtcac tcgctgcagg tattgcttct caggtagcgc
    17161 ataagccacc tcaacggcaa ttttacctag cactgtaaac ctctttcgca cgaaccgtaa
    17221 aagcctggac catattagcc gccagctctt taaacacgcg accaaaggcg agttcaatca
    17281 acttattggt aaactcaaag tcgagatgaa actcgatacg acacgcctcc tggctcagcg
    17341 gcgtaaactt ccatccacca atcaatttct tgaacggccc atccaccaga ttcataagaa
    17401 tactttggtt actggtcaac tggttgcggg tagtaaacgt tttgctgatc ccagccttag
    17461 agacatctac cgccgcagtc atctgcccag gagtggactc cagaatccga cttccggtac
    17521 aacctggcaa aaactgagga taagactgaa cgtcattcac taactgatac atttgctccg
    17581 cgctgtaggg taccagtgcg gtccggctaa tctgaggcat aacaatttcc atcaacaaaa
    17641 atccaacaaa taatatcatt tatcctgctg taaaaaaaac gctatcccgg cgctgggtaa
    17701 catcgggttc atgctaagat agagccttgt cccccgcagg attgatatgg ggtattttcg
    17761 atttcagatt accgatgatt cacgacgctt atgacgaaga aaaaagcaca taaacctggt
    17821 tcagcgacca tcgcgcttaa caagcgcgcc cgtcacgaat actttatcga agaagagttc
    17881 gaagcgggac ttgccctgca aggctgggaa gttaaatccc tgcgcgcagg aaaagccaat
    17941 atcagcgaca gctacgtcct tctgcgtgac ggagaggcat ttctgtttgg cgctaacatc
    18001 acgccaatgg ccgtggcctc cacgcatgtg gtgtgcgatc ctacccgtac ccgcaagtta
    18061 cttctcaacc agcgcgaact ggactcattg tacggtcgcg tcaatcgaga aggctatacc
    18121 gtagtggcgc tctccctgta ctggaaaaat gcctggtgca aagtgaaaat cggcgtcgcc
    18181 aaaggtaaaa aacagcacga taaacgttca gatatcaaag aacgcgaatg gcaggtggat
    18241 aaagcacgca tcatgaaaaa cgcccaccgt taaacctgca ctccaattat tgaccagttc
    18301 ctcaccgcgc ctccctctcc ggcggcgcga atgaacatct tattggctat cacatccgac
    18361 acaaatgttg ccatcccatt gcttaatcga ataaaaatca ggctacatgg gtgctaaatc
    18421 tttaacgata acgccattga ggctggtcat ggcgctcata aatctggtat acttaccttt
    18481 acacattggg gctgattctg gattcgacgg gatttgcgaa acccaaggtg catgccgagg
    18541 ggcggttggc ctcgtaaaaa gccgcaaaaa atagtcgcaa acgacgaaaa ctacgcttta
    18601 gcagcttaat aacctgctta gagccctctc tccctagcct ccgctcttag gacggggatc
    18661 aagagaggtc aaacccaaaa gagatcgcgt ggaagccctg cctggggttg aagcgttaaa
    18721 acttaatcag gctagtttgt tagtggcgtg tccgtccgca gctggcaagc gaatgtaaag
    18781 actgactaag catgtagtac cgaggatgta ggaatttcgg acgcgggttc aactcccgcc
    18841 agctccacca aaattctcca tcggtgatta ccagagtcat ccgatgaagt cctaagagcc
    18901 cgcacggcgc aagccctgcg ggcttttttg tgctccgaat ttgtcccgcg aagtctgatg
    18961 ccaactaatt aaatccgatc cttttaggca ccttgttagg cacttcataa agctatattg
    19021 tctttgaggt gcctaaaact atggaaaccc ggcaatggca agacaaacca aacctctatc
    19081 cgttaaggaa atcgaatctg ctaaacccaa ggaagcggac tacgttctct atgatggcga
    19141 tggccttgag ctactcatta aatccagcgg gagtaaaatc tggcagtttc gctacattcg
    19201 ccctgtcacc aagacacgag caaagaaaag cataggccct tacccgtcag ttacccttgc
    19261 cgatgccaga aactaccgtg cagagtcccg ctcactccta gctaaacaga ttgatccgca
    19321 ggaacatcag caagaacaat tgcgtagttc gctggaagcc aaaaccaata ctttccagct
    19381 cgtagctgaa cgttggtgga atgtgaagaa agccagtgtg acagaggact atgcggaaga
    19441 tatctggcgc tctcttgaaa gagatgtctt tcctgcgatt ggcgacgtta gcgttacaga
    19501 tattaaagct catacactgg ttcaggccgt acaaccggtt caggccagag gagcactgga
    19561 aacggttcgt cgcctgtgtc aacgtattaa tgaggtcatg atctatgccc agaacacagg
    19621 gctgattgat gctgttccca gcgttaatat cggtaaagcc ttcgaaaagc ctcagaaaaa
    19681 gaacatgccc agcattcgac cggatcagct atctcaactg atgcagacaa tgcgaacagc
    19741 cagcattagc ctttccacac gctgcctgtt catgtggcaa cttcttacta ttacccgccc
    19801 tgccgaagcg gctgaagctc gctgggaaga agtagacata gaagcgcaag agtggaagat
    19861 tcccgcagca cgcatgaaaa tgaaccgcga ccatactgtt ccattgtcag atgaagcaat
    19921 agctgtgctg gagatgatga aaccgctaag cggcaaccga gaatttatct ttcctagccg
    19981 tatcaagccc aaccagccta tgaacagtca gactgttaac gcctcactaa aacgcgcagg
    20041 ttttggtggt gtgcttgttt cgcatggtct gagatcaatt gccagtacgg ctcttaatga
    20101 acaaggcttt ccgcctgatg ccattgaggc agcattggcc catgtggata agaatgaggt
    20161 tcgtcgtgct tataaccgca gcgattacct ggaacagcgt cgcccgatga tgcaatggtg
    20221 ggcaaatttt gtgatggcag cggatcgtgg aagcatgatt gaaggtggga taaaaggaat
    20281 gaagctagtt gggt
//
LOCUS       DAJTUI010000073        20187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTUI010000073 DAJTUI010000000
VERSION     DAJTUI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.073"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..717)
                     /gene="aroD"
                     /locus_tag="OO464_002807"
     CDS             complement(<1..717)
                     /gene="aroD"
                     /locus_tag="OO464_002807"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6227892.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISV"
     gene            complement(748..1614)
                     /gene="ydiB"
                     /locus_tag="OO464_002808"
     CDS             complement(748..1614)
                     /gene="ydiB"
                     /locus_tag="OO464_002808"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR6227893.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1626..2898)
                     /locus_tag="OO464_002809"
                     /pseudo
     CDS             complement(1626..2898)
                     /locus_tag="OO464_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3081..4286)
                     /locus_tag="OO464_002810"
     CDS             complement(3081..4286)
                     /locus_tag="OO464_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6227894.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4386..4742)
                     /locus_tag="OO464_002811"
     CDS             complement(4386..4742)
                     /locus_tag="OO464_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR6227895.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4878..4985)
                     /gene="rprA"
                     /locus_tag="OO464_002812"
     ncRNA           complement(4878..4985)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO464_002812"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5171..6283)
                     /gene="ydiK"
                     /locus_tag="OO464_002813"
     CDS             complement(5171..6283)
                     /gene="ydiK"
                     /locus_tag="OO464_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6227896.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6672..9728
                     /locus_tag="OO464_002814"
     CDS             6672..9728
                     /locus_tag="OO464_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6227897.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9725..10135
                     /gene="menI"
                     /locus_tag="OO464_002815"
     CDS             9725..10135
                     /gene="menI"
                     /locus_tag="OO464_002815"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6227898.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10235..10423
                     /locus_tag="OO464_002816"
     CDS             10235..10423
                     /locus_tag="OO464_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6227899.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            10971..11339
                     /gene="sufA"
                     /locus_tag="OO464_002817"
     CDS             10971..11339
                     /gene="sufA"
                     /locus_tag="OO464_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6227900.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11348..12835
                     /gene="sufB"
                     /locus_tag="OO464_002818"
     CDS             11348..12835
                     /gene="sufB"
                     /locus_tag="OO464_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6227901.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12845..13591
                     /gene="sufC"
                     /locus_tag="OO464_002819"
     CDS             12845..13591
                     /gene="sufC"
                     /locus_tag="OO464_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6227902.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13566..14837
                     /gene="sufD"
                     /locus_tag="OO464_002820"
     CDS             13566..14837
                     /gene="sufD"
                     /locus_tag="OO464_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6227903.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14834..16054
                     /gene="sufS"
                     /locus_tag="OO464_002821"
     CDS             14834..16054
                     /gene="sufS"
                     /locus_tag="OO464_002821"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6227904.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16067..16483
                     /gene="sufE"
                     /locus_tag="OO464_002822"
     CDS             16067..16483
                     /gene="sufE"
                     /locus_tag="OO464_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6227905.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16632..17636
                     /gene="ldtE"
                     /locus_tag="OO464_002823"
     CDS             16632..17636
                     /gene="ldtE"
                     /locus_tag="OO464_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6227906.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17699..17935)
                     /gene="lpp"
                     /locus_tag="OO464_002824"
     CDS             complement(17699..17935)
                     /gene="lpp"
                     /locus_tag="OO464_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR6227907.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18246..19658)
                     /gene="pykF"
                     /locus_tag="OO464_002825"
     CDS             complement(18246..19658)
                     /gene="pykF"
                     /locus_tag="OO464_002825"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR6227908.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5288 a         4846 c         5155 g         4898 t
ORIGIN      
        1 taccgagatt tgccctggcg cagaggcttt ttttaccgca ccaaaagttg ccgccgagcc
       61 aaatacttca ccaaccagac gagaaattac gccagttttt gccatcgaca tcgtgatgat
      121 tggacgatcg gcatactgct cctgcatctc cagggtcgcg gcaagcaacg tcagcacatc
      181 gctggtactt tgcggcatca gcgcaatctt aggaatatcg gcgtcgaagg actgcatttt
      241 gcgcagacgg gcaatgattt cttcggcttc cggcgtttta tggaagtcat ggttggacat
      301 gacaactttc acatcatgcg cgtgggcgta ggcgacggtt tctttgacct gatcatcgcc
      361 ggtaaataac tccagatcga tcatatcaac caggccgctg tcgatggctg cacgattgag
      421 agcaatataa gcctcggtgg aaatcgcctg ctcgccgcct tctttggcac tgcggaaggt
      481 aaacagcagc ggtttttctg gcatggtttc acggagaatt tttgccgccg ccatgacaga
      541 ctccacattg gagaggtcgg caaagtggtc cacacgccat tccagaatat caaagtccgc
      601 ttcacgatag gcgagagctt cggatttcac gcgggcgata tctttcgcca tcagcgagac
      661 gatgatttta ggtgcgcccg caccaatgac gagatctttt acagttacgg ttttcatttt
      721 ttaccctttc tgcacgcggt cagcctgtca ggcaccgaac cccatgacct gtttaacata
      781 ttccagaggg aaatctttgc cagtccataa cgtgaactgt tcagcccctt gccacaacaa
      841 catgccgtat ccatcaatcg ttttgcaacc agcttgttgt gcctgctgca ataacttcgt
      901 catatgcggg ttatacacgc attcagtgac cagcagtccc ggatgtaaca gactgatatc
      961 attaaccaat gattcattct caaggggttt catacctact tttgtgccat tggttaaaat
     1021 gtcggcggaa gccagggctt cagcaaaggc ttgctgatcg gcgagatcgg tatccgtgac
     1081 gacacaatcg gtgttttcgt taacccgctg cgcgaaggcg agggctttat cgaagaactc
     1141 atcccgacgg ttaaagagtt taatttcttt taaaccttca attgccccct gcgcgccaat
     1201 cgccgttgag gcacctccgg cccctaacag caccatcgtt ttgcctttga tatcaaaacc
     1261 gctctcttta atggcgcgaa tatggcccgt gccgtcggtg ttatagccac gcagatagcc
     1321 atcatcatta acgatggtgt tgatggcacc caccagtttg gctgctggcg ttaattcatc
     1381 aacatattca cacgccagtt gtttgttcgg catcgacaca ccagttccgc gcattttgag
     1441 ggcttttaat ccttcaattg ctccgggaaa gctatcgtta tccacttcga aggccatata
     1501 ggtaaatggc aatcccgctt tttctaaggc tttattctgc atttcaggcg ataaactgtg
     1561 gcggatagga taagccatca atccaatcaa ttcgtatttt gcggtaacat ccataattaa
     1621 ctcctttaac ctctatgctt aattgtgttt aaccgggtgc taaatttgcg ttcgccaaac
     1681 cgcacatcat tctcaggaat aataaaaacg cggtaatagc ggctaaaaac aataattgcg
     1741 gtaatcaggg ccagcagcgc gaaagtaaaa tcgagaacaa tgatatattg caggccgatg
     1801 ttcgacagat aaccggtaat cagcggaata acaaagttag ccagtccacc catcatcata
     1861 taaatactgg tgactttggc tttgcttttg gggaaaaact ctgacatgac cgaaacaccg
     1921 agctgtaaaa tgccgccagc tgctgagaaa ccgataacaa aggcgccggc attacacacc
     1981 agtggagaag ggtacaggta gataatggca gctgttattg ttgccagcgc agagttaaat
     2041 acattagccc agatgggccg gaccattttt ttcagtagtg cggcaaaaat aaagacacag
     2101 accaacgagc ccatactgta ataagagatg gtttttaatg cctcagcttc tgacatacca
     2161 gcaaaagcca tcgcatattt gggcatccac accacaatca cataaaaggt cgagaatgcg
     2221 gctacaccga acagtaccga tgaaacacct tccagccaga ctaaaggttt tgctgttcat
     2281 ttgcggtaat tcattagcta cgctggcgtc caccaactgg ctggggaatt tgcttttcaa
     2341 cagcatcagc gtgatcagta caaacagaat acccggaata atcagcccat agccgtacca
     2401 gatattattg agcaacatat agctcaccag cattgggtag aacatttgcc caaatgacac
     2461 catcgcttta accagtatga ccgccgaacc agaggctttc ggaaagcatt ccatgagcgc
     2521 ggggtagcca cccgtatcca gcgctgagtt agcgatacct acgcacactg ccagaccgta
     2581 ggcgagagtt aaattcgggc aagcgggaat accaaagaag aatagcagat acattattac
     2641 tgccattaat atcaccgccc gacgaccaaa cttatcggag atcacaccga agaataaaat
     2701 actgatcaat cgccccaaac cgataccgga aattaaatag gcaatgcccg cgttgtcagt
     2761 ggaaaacttt tctgacagag atgtcatatt ttgggcaagc gtaataacac taatgccgtg
     2821 caggaaatag ctgaagtaaa tacaaagaac agccaggata aatggcgtgc tgaaagataa
     2881 tttcgttacc gtacgtattg ttctccgacg gtttacataa ttactatact gaagatgatt
     2941 ttccctgggt acatggcata aagtttatgt ttccaggaat attgcatgtc atagtttgtt
     3001 tttgatcgcg attttcctta gttagcgcag aaagagttat ttatccgtaa gatcttgcat
     3061 gacctgaaaa gaatattgca ttacccaccc ggagcgacat tttcctttag cgagtgatgc
     3121 cgcgtttttt tgcggctgcg taagccgata aacagtgcca gtaaaaaacc gatggcagta
     3181 tcgaaccaca taatatcggc aatacttctt tgggacagat gagccgtaat caacggaata
     3241 gtaaaggtcg caatactgcc cgtactgtaa tagatccctg tagctttacc tttagcgtaa
     3301 gggaaacgtt cagccattaa cgtcaggcca atttgcacaa cacctccagc agaggtaaaa
     3361 ccaatgacaa aagcaaatat tatcaccaca taaaatgtgg gatgcaggca gacggtaaac
     3421 agagcaataa atgagataaa ggtgtacagc atcagtaatg ttgttgggcg aacggtatta
     3481 cgaatgagtg gagcggtaat aaatacacaa agcagcgaac ccacggtgta gatactgagt
     3541 agtttgatcg acatagtgta tgacatgcct gcaacaaatt gtccgtactg tgccagccac
     3601 tggctaacca gataaaacgt tgccattgag atatagccat ataaggtata actggctaaa
     3661 tcgataattg aacagcgatg ttccgtagag ctggtggttt tctttatgac aggtaagcga
     3721 cgacccggat ggggtgggaa cgtacaacgg tataaaaaca gagcgttaat aaacataatg
     3781 cctgcagcaa tcataaagga ccaaccgaac cacagttcag cccacactaa caggctaatg
     3841 attagcggta ataaaaattg tccgctggaa acaaatgctt taattaaaat attggctgtc
     3901 ccaggtgagc gtggaaaagc ttccatcaaa ctgggataag tgcctgcatc gagaaaactg
     3961 tttgccattc ccgccagaaa gccaaaaaca taagcgataa tgatgttatt ggtctgcagg
     4021 atgtcaaaaa agaaggccat atagcagcac atcccgagca tgataaaagg gcggcgacca
     4081 aagcgatcgg ataataatcc tgcaaaaagc aagacactta atcgaccaat gcccagcgat
     4141 gagataacta tcgagacacc cgcggcatta gtctgccaaa gtgtctccag cgaggccata
     4201 ttcaggctca tcaaaatgac gcccatacca tgcaccaggt aattaaaata caacccaagt
     4261 gcggtagggt aatagggatt tttcataggt tctacttagc aaaggtgctt taaaaagtgt
     4321 agcgatgttg tgtcagtatc cattcattat tgttgtaaca acgacaattc ctttctgtgg
     4381 aaaaattaac aaagccgctc cagatcgtgt gcatataatt cagcggctac cgattgatag
     4441 atcttccagt caataaaatt gccatcaggg tagacttgtt gaaacgcggt taagtcgggg
     4501 aaaaaacgca tggtgttgtt gccgatgcgg ttatttattt tctctatgat ggcatgaaga
     4561 tgtttttttc gttgctgacg catagccaac agttgctcga ctacacaatc aggaatggcg
     4621 catttgccat tttcccactg acgccagctt tcactgtcac cggtctgggc aatccaggtc
     4681 gcgcattcat caatagtcat ggcaaaaata tggcggagtg cctggagttc ataagcgttc
     4741 atcgtgtaat ggctgcatga aataaacaat aaaatataaa taacattatc agcctgctga
     4801 cggcttgaag agagtcacag tatcttgtgc aacattattg gaaagagtga ggggcgaggt
     4861 agcgaagcgg aaaaatgtta aaaaaaagcc catcgtggga gatgggaaaa gactacacac
     4921 agcaattcgt tgtttcactc aggggatttc catgcttata aatcaatatg ttgatttata
     4981 accgtgagct aatagtaggc atacggactt tttgcgtcga tcagattcgt ctcattagtt
     5041 taaaaactgt aaagaatttg cgacaggtac taccagctga atggcggtaa gcaggaaaat
     5101 atggggaaga gcaagcaaat atcaattagc ggtgattaac aatcaggcgg cagagccgcc
     5161 tgatgagaaa ttacttattc ggtttctcga tttcgccaag ttcttcgaga atttcttccg
     5221 gttggtccgt cggtggcggg acttcttcca cccacgcggc aaacagacgc caggaaacgg
     5281 ctaacagaac cggaccaata aacagaccga tcatcccgaa agcaatcaaa ccaccaataa
     5341 cgccagagag gatcaggatc agcggtaaat cggcacccat gcgaattaac attgggcgga
     5401 tgacgttatc cagcgtgcca accacaccgc tccacactaa caataccgtt ccccaggtgg
     5461 tatcgccggt ccagtagagc cagataatcg ccggaatcag taccggcaac gggccaagct
     5521 ggacaaggca ggagaggatc attaacaccg ttagcaacgt tgcataaggt acgccggata
     5581 cggcgaggcc gataccgcca agcactgcct gtactaacgc cgtcaccacc acacccagcg
     5641 ccaccgcgcg gatagcctgt gccgccagca gcacggcggc atctccgcga acgcctgcca
     5701 gacgggttgc aaaatggcga atgccttgtg ccacctgttc accgcgccag tacagcaggg
     5761 cactgaagag aagcatcagc gcacaatgca ccataaagcg cccgatatgc gccgcctgcc
     5821 caacgaacca ggtggtggtg gtgccaatat aagggcggac tttcgccatg atcgccgtac
     5881 cccccatatc cagcaagttg tgccagcctg catacagctt cgcgccaatc accggaatgg
     5941 tattaagcca cgccagatcg ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg
     6001 ggccgctgcc gtcgacgata ctgttaacca gcaaagcaat agggatgata aacaccatca
     6061 ctaataacag cgtcatcacc agaacggcga gagagcggcg gccaaacatg atcttttgca
     6121 aacgtaacaa taccggccag gtggcgataa ccaccgtacc ggcccatgca aagccgagaa
     6181 taaagggttg aacaatccac agacatgcca caatcatgat ggctaaaaac agcaccgaaa
     6241 gcagaatttg tgcgacatcc ctgggctgac gaacatttac cataactact tttcaccttt
     6301 tttgtgcgtc aaatcgtcga cgcgacgtga acacgcgaaa ctcacctgca taatgatgag
     6361 caatttcagc ggtttttaac aggccgattc tgcccttaat tctgatgggc gtgttagaaa
     6421 aaaatgtggt acaacaactc aggcaacacg caaacgattt actcgtcgta tttcaagttg
     6481 catgtgctgc ggctacgttc gttcacccca gtcacttact attgtaagct cctggagttc
     6541 actcgcttgc cgccttcctg caactcgaaa tccatagagt aaataaacat cacctttcag
     6601 tgcgctattg cagccgcctg aaagaatagt gtatcgacct acatcacaga ccgcaggaaa
     6661 gggtcaatat aatgattcca cagatttccc tggcacccgg cgtcgttcaa ctggtgctta
     6721 attttttgca agagctggag caacaaggtt ttaccggcga tgcggcgaca agttatgccg
     6781 atcgtctgac aatgtcgacc gacaacagta tttaccaact tctccccgat gcggtggtat
     6841 ttccgcgttc aaccgcagat gtggcgctga tcgcccgtct tgccgcgcag gaacgctatt
     6901 catcgctgat ctttaccccc cgcggcggcg gcaccggcac taacggtcag gcgctcaacc
     6961 aggggattat tgttgatatg tcccgccata tgaaccgcat catcgaaatt aaccctgaag
     7021 agggctgggt gcgcgttgag gccggggtga taaaagacca actcaatcag tacctgaaac
     7081 cgttcggcta cttttttgcg ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga
     7141 tgatcaatac cgatgcatcc ggtcagggat cgctggtcta tggcaaaacg tcagatcacg
     7201 tacttggcgt acgcgcggtg ttgttggggg gcgatattct cgatacgcaa cctttacccg
     7261 tcgagctggc ggaaacgctg ggtaaatcca ataccacaat cgggcgaatt tataacacgg
     7321 tttatcaacg ttgccgtcag caacgccagt taatcatcga caacttcccc aaacttaacc
     7381 gctttcttac cggttacgat ctgcgtcatg tctttaacga tgagatgacc gagttcgacc
     7441 tgacgcgcat tctgacgggt tcagaaggga cgctggcctt tattaccgaa gcgcggctgg
     7501 atattacgcg cttgcctaaa gtgcgccgtc tggtgaatgt caaatatgac tcttttgact
     7561 ctgcgctgcg taacgcgccg tttatggttg aggcgcgggc gctttcggta gagacggtgg
     7621 actcaaaagt gctgaatctg gcgcgggaag atattgtctg gcattccgtc agcgagttga
     7681 ttaccgatat gcctgacaaa gagatgctcg gactgaacat tgtggaattt gctggtgatg
     7741 atgaggcgct gattgacgag cgagttaatg cactctgtgt gcggcttgat gagctgattg
     7801 ccagccaaca ggcaggtgtg attggctggc aggtgtgccg cgagctggcg ggggttgaac
     7861 gtatctatgc gatgcgcaaa aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta
     7921 agccaattcc gtttgctgag gatacctgcg taccgccgga acatctggcc gattatattg
     7981 ctgaatttcg cgcgctgctc gacagccacg gcttaagcta cggtatgttc ggtcacgtcg
     8041 acgcaggtgt cttgcacgtc cgtccggcac tggatatgtg cgatccgcaa caagagattt
     8101 tgatgaagca aatctctgat gacgtggtgg cgctgactgc gaaatacggt ggtttgttgt
     8161 ggggcgagca cggcaaaggt tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac
     8221 tttttgcaga actgcgcaaa gtgaaagcgg catttgaccc gcataaccga ctcaacccag
     8281 ggaagatttg cccgccagaa ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc
     8341 gcggtacatt cgatcggcag atccccattg cggtacgcca gcagtggcgc ggtgcgatgg
     8401 agtgtaacgg caacggttta tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga
     8461 tgaagatcac ccagaaccgg attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat
     8521 ggctgcgctt gttggcggat cgcggcgttg acccactcaa actggaacaa gaactgcctg
     8581 aatccggcgt cagtttgcgg acgttaattg cccgcacgcg caatagctgg catgcgaata
     8641 aaggcgaata tgacttctca cacgaagtca aagaggcgat gtcgggctgt ctggcctgta
     8701 aagcttgttc gacccagtgc cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc
     8761 tgcagctcta tcacacccgt tatttacgcc cgcttcgcga ccacctcgtt gctacggtcg
     8821 agagctacgc accgctgatg gcacgcgcgc cgaaaacctt taacttcttc attaaccagc
     8881 cgctggtgcg caaactctcg gaaaaacata tcggcatggt tgatttgcca ctgctgtcgg
     8941 tcccctcgct acaacaacaa atggtggggc atcgctcggc aaacatgacg ctggaacagc
     9001 ttgaagctct caatgcagag cagaaagcgc gcacggtgtt ggtggtgcag gaccccttta
     9061 ccagctatta cgatgcgcaa gtggtggcgg attttgtccg tctggttgaa aaattaggtt
     9121 tccagcctgt gttactgcca ttttcgccaa atggcaaagc ccagcatatc aaaggctttc
     9181 ttaatcgttt tgcgaagacg gcgaaaaaga cggcggattt cctcaaccgt atggctaagc
     9241 ttgggatgcc aatggtgggc gtcgatccgg cgctggtact ttgttatcgc gatgaatata
     9301 aactggcgct gggcgaggaa cgtggcgagt ttaacgtctt acttgcgaat gaatggctgg
     9361 caagcgcact tgagtcacat ccagtggcta cagtcagcgg tgaatcatgg tatttctttg
     9421 gtcactgtac cgaggttacc gctttgccgg gcgcgccagc acaatgggcc gcgatatttg
     9481 cccgttttgg cgcgaaactg gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt
     9541 acggacatga agcgaaaaac catgaaaatt cgctcgggat ctatgagtta tcctggcatc
     9601 aggctatgca gcgactgccg cgtaaccgct gtctggcgac cggatattcc tgccgtagcc
     9661 aggtaaaacg ggttgaaggc acgggggtac gccatcctgt gcaggcttta ctggagatta
     9721 ttaaatgata tggaaacgga aaatcaccct ggaagcactg aatgctatgg gtgaaggaaa
     9781 catggtgggg ttcctggata ttcgctttga acatattggt gatgacaccc ttgaagcgac
     9841 aatgccagta gactcgcgga caaagcagcc tttcgggttg ctgcatggag gagcatccgt
     9901 ggtactggcc gaaagtatcg gttccgttgc cggttattta tgtaccgaag gtgagcaaaa
     9961 agtggttggt ctggaaatca atgctaatca cgtccgctcg gcacgagaag ggcgggtgcg
    10021 cggcatatgt aaaccgttgc atctcggttc gcgtcaccag gtctggcaga ttgaaatctt
    10081 cgatgagaaa gggcgtttgt gctgttcgtc acgattgacg accgccattt tgtgacgttg
    10141 tcaccagaaa agtgtgacgc atattgcttt tggtcattat ctgcacagta aacggtggta
    10201 tactgtgcag gtcttgtcta aaagcgagga tgctatgtct actcaacttg atcccaccca
    10261 gctggccatt gaatttttac gccgtgacca gagcaatctt tcacctgccc aatatctcaa
    10321 acgtctgaaa caactagaac ttgaatttgc tgacctgctt actctctctt ctgctgaatt
    10381 aaaagaagaa atctacttcg cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc
    10441 aagttcgcca aatcgacctg gattaacctc cgtccaggtt cgatgacctt cttaatactg
    10501 cttatctacc gcctgatatt gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc
    10561 gggttagctt tatgagatta ttcttatcgc cccttcaaga gctaagccac tgagagtgcc
    10621 ggagataagc gccggatggg gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt
    10681 ttcttatttc tgaacgctgc acttttttac ctatctctta aatagtcatt ttcaatacaa
    10741 ctttttcttt ttcatcacag ccttaatgct ccttagataa taaccattat ctaacaatga
    10801 gatacctaat tcttagcgtg cctgttaacc cacacatcag gggctatgct tattaaaagc
    10861 aggcaaaaag aatgttttac ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat
    10921 tatcactaac atgctgttat acgctgaaag cgatgaagtg aggtaaatag atggacatgc
    10981 attcaggaac ctttaaccca caggatttcg cctggcaagg cttaacgctg acacccgcag
    11041 cggcgataca catccgcgag ctggtggcaa agcagccagg tatggtcggc gtgcgcttag
    11101 gtgtgaagca aacgggctgc gcgggctttg gctatgtgct cgacagtgtt agcgagccgg
    11161 acaaagacga tctgctgttt gaacacgacg gcgcgaagct gtttgtcccg ctgcaatcga
    11221 tgccgtttat tgatggcacg gaagtcgatt tcgttcgtga aggacttaat cagatattca
    11281 aatttcacaa ccctaaagcc cagaatgaat gtggctgtgg cgaaagcttt agggtatagg
    11341 cggtactatg tctcgtaata ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc
    11401 gctgaactat aaagaaggat tcttcaccca gttagccacc gatgagctgg caaaggggat
    11461 aaacgaagag gtggtgcgcg caatttcggc gaagcgtaat gagccggagt ggatgctgga
    11521 gtttcgtcta aacgcctatc gcgcatggct ggagatggaa gaaccgcact ggttgaaagc
    11581 gcactacgac aagctgaatt atcaggatta cagctactac tcagcaccat cgtgcggtaa
    11641 ttgtgacgac acttgcgcgt ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt
    11701 tttaagtaaa gaggtggagg cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa
    11761 agaggtggcg gtggatgcca ttttcgactc tgtttcggtt gccaccactt atcgcgaaaa
    11821 actggcggag cagggaatta ttttctgttc ctttggtgag gcgatccacg atcacccgga
    11881 actggtgcgt aaatatctcg gcaccgtggt gccggggaat gacaacttct ttgcagcact
    11941 taatgcggcg gtagcctctg acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc
    12001 gatggaactt tccacctatt ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac
    12061 aattctggtg gccgacgaag acagctacgt cagctacatt gaaggctgtt ccgctccggt
    12121 gcgtgacagc tatcagttgc acgcggcagt ggtggaagtc atcatccata aaaacgccga
    12181 ggtgaaatat tccacggtac aaaactggtt ccctggcgat aacaacaccg gcggtattct
    12241 caacttcgtc accaagcgtg ctttgtgcga aggcgaaaac agcaaaatgt catggacgca
    12301 atcagaaacc gggtcagcga ttacgtggaa atatcccagc tgcattttgc gcggcgataa
    12361 ctccattggt gagttttact cagtggcgct gaccagcggt catcagcaag cggataccgg
    12421 caccaagatg atccacatcg gtaaaaacac caaatcgacc attatctcga aagggatctc
    12481 tgccggacat agtcagaaca gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa
    12541 tgcgcgcaat ttcactcagt gcgactcaat gctgattggc gctaattgtg gggcgcatac
    12601 cttcccgtat gtcgaatgtc gcaataacag cgcacaactg gagcacgagg caacgacatc
    12661 acgtattggt gaagatcaac tgttttactg cctgcaacgc gggatcagcg aagaagacgc
    12721 catctcgatg attgttaacg gtttctgcaa agacgtgttc tcagaactgc cgctggaatt
    12781 tgccgttgaa gcacaaaaac tcctcgccat cagtcttgaa cacagcgtcg gataaggaat
    12841 aaacatgtta agtattaaag atttacacgt cagcgtggaa gataaagcta tcctgcgtgg
    12901 attaagcctc gacgttcgtc ccggcgaagt tcacgccatt atggggccaa acggttcggg
    12961 caaaagtacc ttatcggcaa cgcttgccgg gcgcgaagat tatgaagtga cgggcggcac
    13021 ggttgagttc aaaggcaaag atttgcttgc actgtcgccg gaagatcgtg caggcgaagg
    13081 catctttatg gccttccagt atccggtgga gattccaggc gtcagtaacc agtttttcct
    13141 gcaaacggca cttaatgcag tgcgcagcta tcgcggccag gaaacgctcg accgctttga
    13201 ttttcaggat ttgatggaag agaaaatcgc tctcctgaag atgccggaag atttattaac
    13261 ccgttcggta aacgttggtt tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat
    13321 ggcggtgctg gaaccggagt tatgcattct tgatgagtcg gactccgggc tggatattga
    13381 cgcattaaaa gtggtcgccg atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat
    13441 cattgttacg cactaccaac gcattctcga ctacatcaag cctgattacg ttcatgtgct
    13501 atatcaggga cgaattgtga aatccggcga tttcacgttg gtcaaacaac tggaggagca
    13561 gggttatggc tggcttaccg aacagcagta atgcgctgca acagtggcat cacttgtttg
    13621 aagctgaagg gacaaaacgc tcaccgcaag cacagcagca tttacaacaa ttgctgcgta
    13681 ccggactgcc gacacgtaaa catgaaaact ggaaatatac gccgctggaa gggctgacca
    13741 atagtcagtt tgtcagcatt gcgggagata tatccccaca gcagcgtgat gccttagcgt
    13801 taacgttaga cgccgtgcgg ctggtgtttg tcgatggacg ttacgtgccc gcgctgagcg
    13861 atgcaattga aggcagcgga tatgaagtga gcattaacga cgaccgtcag gggttacccg
    13921 acgctattca ggcggaagtg tttctgcatt tgacggaaag cctggcacaa agcgtgacgc
    13981 atatcgccgt gaagcgcggt caacggccgg caaagccatt gctgttaatg catatcaccc
    14041 agggcgtggc aggtgaagag gtgaacactg cccattaccg gcatcatctg gatctggcgg
    14101 aaggtgccga agcaacggtg atcgaacatt ttgtcagcct taatgatgct cgtcatttta
    14161 ccggggcacg gttcactatc aatgtcgcag cgaacgccca cttgcagcat atcaagctgg
    14221 cgtttgaaaa cccggtcagt caccactttg cccataacga tttgttgctg gctgacgatg
    14281 ccaccgcatt tagccatagt ttcctgctgg gtggcgcagt gttacgacac aacaccagta
    14341 cgcaactcaa tggcgaaaac agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa
    14401 acgaggtgtg tgatacccgt acctggctgg aacacaataa aggtttttgt aacagccgac
    14461 agttgcacaa aactatcgtc agcgacaaag gccgcgcggt atttaacggt ttgatcaacg
    14521 tcgcgcagca cgccatcaaa acggatggtc agatgaccaa caataatctg ctgatgggca
    14581 aactggcgga agtggataca aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca
    14641 gccacggcgc gacggtgggg cgtattgatg atgaacagat gttctatctg cgctcgcgcg
    14701 ggatcaatca gcaggatgcc cagcagatga tcatttacgc ctttgctgcc gaactgacgg
    14761 aagcactgcg tgatgagggg cttaaacagc aggtgctggc ccgaatcggt caacggctgc
    14821 caggaggtgc aagatgactt tttccgtcga caaagtgcgg gccgactttc cggtgctttc
    14881 gcgtgaggta aacggtttgc cgctggctta tctcgacagc gccgccagtg cacagaaacc
    14941 gagccaggtg attgacgccg aggccgagtt ttatcgtcat ggctacgcgg cggtgcatcg
    15001 cggtattcat accttaagcg cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc
    15061 atcgctgttt attaatgccc gttcggcgga agagctggtg tttgtccgcg gcacgacgga
    15121 agggatcaat ctggtcgcca atagctgggg caacagcaat gtgcgggcgg gcgataacat
    15181 catcatcagt cagatggagc accacgctaa cattgtcccc tggcagatgc tttgcgcacg
    15241 cgttggcgca gagctgcgtg tgatccctct caaccccgac ggtacgctgc aactggagac
    15301 gctgcctacg ctgtttgatg agaaaactcg cctgctggca attactcatg tctccaacgt
    15361 gcttggcaca gaaaatccac tggcggaaat gatcacgctt gcgcaccagc atggcgcaaa
    15421 agtgctggtg gatggcgctc aggcggtgat gcatcatccg gtggatgttc aggcgctgga
    15481 ttgcgacttt tacgtgttct ccgggcataa actgtatggc cccaccggaa ttggcattct
    15541 ttatgtgaaa gaagccttgt tgcaggagat gccgccgtgg gaagggggcg gttctatgat
    15601 cgccaccgtc agcctgagtg aaggcactac ctggaccaaa gcaccatggc ggtttgaagc
    15661 cggtacaccc aataccgggg gcatcattgg tcttggcgcg gcgctggagt atgtttcggc
    15721 gctggggctt aatagcatag ccgagtatga acagaatctg atgcattatg cgctatcaca
    15781 gctggaatct gtaccggatc tcactctcta tggccaacaa aacaggcttg gcgttattgc
    15841 ttttaatctc ggtaaacacc acgcctatga tgttggcagt tttctcgata attacggcat
    15901 tgctgtgcgt accggacatc actgcgctat gccattaatg gcctattaca acgtccctgc
    15961 gatgtgtcgg gcgtcgctgg ccatgtataa cacccatgaa gaagtggagc gtctggtgac
    16021 cggcctgcaa cgtattcacc gtctgctggg ataacaggga ggcactatgg ctttattgcc
    16081 ggataaagaa aaattgctgc gtaatttttt acgctgcgcc aactgggaag agaaatatct
    16141 ctacattatt gagctgggcc agcgtctgcc agaattacgc gccgaagacg gaagcccaca
    16201 aaatagcatt cagggctgcc agagtcaggt gtggattgtc atgcgccaga atgcgcaggg
    16261 aattattgaa ttgcagggcg acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt
    16321 ctttattctc tacgatcaga tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg
    16381 gtttgaaaaa atggcgctca cccaacatct caccccatct cgttcacaag gtctggaagc
    16441 gatgattcgc gcaattcgcg ccaaagccgc tgcacttagc taaactaaag ggacagcttt
    16501 catccggttg gctcacattg ccggatggtc aatgtttaca ctgaccggca caggagtgtt
    16561 ttggtttcag gtgaacataa gcaccatccg acgtttcagc gtgagtctcc ggcaaataca
    16621 ggaggtttac aatgaaacgc gcgtctttgc ttacactcac gcttatcggc gcttttagcg
    16681 ccatccaggc tgcctgggcg gttgattatc cgctaccacc aaccggaagc cgactggttg
    16741 ggcaaaatca aacgtatacg gtgcaagaag gggataaaaa ccttcaggct attgcccgac
    16801 gttttgatac tgcagcaatg ttgatccttg aagccaataa cactatcgcc ccggtgccaa
    16861 aacctggtac gacgataact attccttcac aactgttatt acctgatgca ccgcgtcagg
    16921 ggattatcgt taaccttgca gagctgcgcc tttattatta tccgccggga gaaaatattg
    16981 tgcaggttta tccaataggt attggattgc aggggctgga aacgccggtg atggaaacgc
    17041 gtgttgggca gaaaatccct aacccaacct ggacgcctac ggcaggcatt cgtcagcgtt
    17101 cgctggagcg tggcattaaa ttaccgccag tcgttcctgc cggaccaaat aacccgctag
    17161 gacgttacgc actgcgcctc gcgcatggta atggcgaata cctcattcat ggcaccagtg
    17221 cgccggacag cgttggtttg cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata
    17281 ttaaagcctt gttctccagc gtgcggacgg gaacgccggt gaaagtgatc aacgaaccgg
    17341 tgaaatattc cgtggaacct aacgggatgc gttatgttga agtacatcga ccactatcag
    17401 cagaagaaca gcaaaacgtt cagacaatgc catacacact gccagcaggc tttacgcaat
    17461 ttaaagacaa taaggctgta gatcagaagt tagtcgataa agcgttgtat cgtcgggcag
    17521 ggtatccggt ttcggtgagc agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag
    17581 agtcagcgca gaatggtgaa ccagagcaag ggaatatgtt acgcgcgacg cagtagtggt
    17641 aaatagcaga caaaaaaatg gcgcacaatg tgcgccattt ttcacttcac aggtactatt
    17701 acttgcggta tttagtagcc atgttgtcca gacgctggtt agcacgagct gcgtcatctt
    17761 tagcagcctg aacgtcggaa cgcattgcgt tcacgtcgtt gctcagctgg tcaactttag
    17821 cgttcagagt ctgaacgtca gaagacagct gatcgatttt agcgttgctg gagcaacctg
    17881 ccagcagagt agaacccagg attaccgcgc ccagtaccag tttagtagct ttcattatta
    17941 ataccctcta gattgagtta atctccatgt agcgttacaa gtattacaca aagtttttta
    18001 tgttgagaat atttttttga tgggaaggca cttatttttg atcgttcgct caaagaagca
    18061 tcgaacgctg ggttttagat aaaaaaattg agagaaaaca ggatgcttcc atcggattca
    18121 tcttaaataa agtgagatta tatagtgaaa tccgatttga ttttttattg cttctggtta
    18181 tcgattaaat aaaaaaagcg cccatcaggg cgcttcgata tacaaattaa ttcacaaaag
    18241 caatattaca ggacgtgaac agatgcggtg ttagtagtgc cgctcggtac cagtgcacca
    18301 gaaaccataa ctacgacgtc acctttgtgt gccagaccgc tctgcagagc cagttcttta
    18361 cccagacggt agaaatcatc agtagaagtg atctctttaa ccagctgcgg cacaacgcct
    18421 ttgctcagta ccaactgatg agccgttttt tcgttggtgg tcagtgccag gatggtggca
    18481 tccgggaagt atttacgtac tgcgcgagca gatttaccgc cctgggtagc aaccacgatc
    18541 agcggagcat ccagtttttc agcagtttca acggcaccac ggcataccgc ttcggtgatg
    18601 cgcagtttac ggttgtcatt gttgaactcg agacggctgt tcatcacgcg gtcggtacgt
    18661 tcgcagatgg tcgccatgat agaaaccgct tccagcgggt atttaccttt tgcggattca
    18721 ccagacagca tcactgcgtc agtaccgtcg aggatggcgt ttgcaacgtc accggcttct
    18781 gcgcgagtcg ggcgtgggtt tttgatcatg gaatccagca tctgggtcgc agtgataacg
    18841 actttacgtg cacggataca tttttcgatc atcatcttct gggcgaagat aacttcttct
    18901 accgggattt ctacacccag gtcgccacgc gcaaccatga tgccgtcaga ggcttcgagg
    18961 atttcgtcga agttgttgag gccttcctgg ttttcgattt tggagatgat gtggatgttt
    19021 tcgccgccgt gcgctttcag gtgctcacgg atttcgataa cgtcagaacg cttacgaata
    19081 aaggaagcag caacaaagtc tacgcctttt tcgcaaccaa agatcaggtc ctgtttgtct
    19141 ttttcagcca gtgctggcag agcaatggaa acgccaggca ggttcacacc tttgttttcg
    19201 cccaggtcac cgttgttcag cactttacag ataactttgt taccttcaat ggcggtaact
    19261 tccataccga tcagaccatc gtcaaccagt acggtgttgc caacagacag gtcagtagtg
    19321 aaaccttcat acgttaccgc aaccatttcg ctgttgccga taacagattt atcagtggtg
    19381 aaagtaaagg tctgaccagc tttcagagaa acatcgttac cgccttccag tttcatggtg
    19441 cggatttccg gacctttggt atcaagcagg atagcggcgg ttttaccagt tttgctcatc
    19501 acgttgcgca gattctgaat gcgctgaccg tgttctgcat agtcaccatg agagaagttc
    19561 agacgcataa cgttcatgcc agcgtccagc attttagcta acatctcttc agattcggtt
    19621 ttcggtccga tggtgcaaac aattttggtc tttttcatga cagtcttagt ctttaagttg
    19681 agaaggatgg gagaaacttg ctttctgggc gcactgccgg aaagcgaatc cggtattacg
    19741 aaagtggtgg tgcctcgctc taaggatagg tgacatcgaa aaagcgtgca gaggaatgtg
    19801 tgcttgtgtc tcagcccaac gtgagtgtct gtttttgagt cgttgagttc tacagtccaa
    19861 agtgctgaaa ccattcaaga gtcaattggc gcgcattata cgctgaaatt aatccaaaag
    19921 gaaaacaaaa acagcgtttc aaaaaatata caacttgtta gacaataaat tgttatcaaa
    19981 gatttaacgt tccagggaaa aggttacgca aaaacttcgc tgtttgcgca acgtctggtg
    20041 ccgaagttgt cagtgtgagg ataagtgaaa gttcatccgg tgtataaata tatcatcgct
    20101 caattgctac agttaaaata atttatcgcg ttatttgtct gattttgttt gatttttgtt
    20161 taattggtta gattgatata tgctgat
//
LOCUS       DAJTUI010000074        19698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTUI010000074 DAJTUI010000000
VERSION     DAJTUI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.074"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO464_002826"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO464_002826"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6227909.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO464_002827"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO464_002827"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6227910.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO464_002828"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO464_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6227911.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="OO464_002829"
     CDS             6038..6658
                     /locus_tag="OO464_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6227912.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="OO464_002830"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="OO464_002830"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6227913.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO464_002831"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO464_002831"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6227914.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO464_002832"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO464_002832"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6227915.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="OO464_002833"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="OO464_002833"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6227916.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO464_002834"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO464_002834"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="OO464_002835"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="OO464_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="OO464_002836"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="OO464_002836"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6227917.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="OO464_002837"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="OO464_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6227918.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO464_002838"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO464_002838"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6227919.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="OO464_002839"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="OO464_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6227920.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="OO464_002840"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="OO464_002840"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6227921.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="OO464_002841"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="OO464_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6227922.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4683 a         5506 c         5031 g         4478 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtatcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DAJTUI010000075        19224 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTUI010000075 DAJTUI010000000
VERSION     DAJTUI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.075"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..2012
                     /gene="pbpC"
                     /locus_tag="OO464_002842"
     CDS             <1..2012
                     /gene="pbpC"
                     /locus_tag="OO464_002842"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR6227923.1"
                     /translation="AAWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKILQLWRAL
                     QLEWHLSKREILILYLNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQVP
                     SRLRPDRWPERAEAARNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSR
                     MMLGKSKSDKIVTTLDAGLQRRLEELAQNWKGRLPPRSSLAMIVVNHTDMRVRGWVGS
                     VDLNDDSRFGHVDMVNAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPG
                     NFDSGFHGPINMSEALVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLS
                     LILGGAGAKLEDMAAAYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADE
                     AQPLPDSALPRVAPLAWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFA
                     SAVPLLNQVNNILLSRSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLL
                     DGSQPPTLLLSEQEGINGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPAS
                     ERRAVRLPPASTICPPYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGER
                     WWFLNGEPLTERGRNVTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            2162..2593
                     /gene="ndk"
                     /locus_tag="OO464_002843"
     CDS             2162..2593
                     /gene="ndk"
                     /locus_tag="OO464_002843"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6227924.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2743..3897
                     /gene="trmG/rlmN"
                     /locus_tag="OO464_002844"
     CDS             2743..3897
                     /gene="trmG/rlmN"
                     /locus_tag="OO464_002844"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6227925.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4182..5195
                     /gene="rodZ"
                     /locus_tag="OO464_002845"
     CDS             4182..5195
                     /gene="rodZ"
                     /locus_tag="OO464_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6227926.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5222..6349
                     /gene="ispG"
                     /locus_tag="OO464_002846"
                     /gene_synonym="gcpE"
     CDS             5222..6349
                     /gene="ispG"
                     /locus_tag="OO464_002846"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6227927.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6452..7726
                     /gene="hisS"
                     /locus_tag="OO464_002847"
     CDS             6452..7726
                     /gene="hisS"
                     /locus_tag="OO464_002847"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6227928.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7744..8364
                     /gene="yfgM"
                     /locus_tag="OO464_002848"
     CDS             7744..8364
                     /gene="yfgM"
                     /locus_tag="OO464_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6227929.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8375..9553
                     /gene="bamB"
                     /locus_tag="OO464_002849"
     CDS             8375..9553
                     /gene="bamB"
                     /locus_tag="OO464_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6227930.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9671..11143
                     /gene="der"
                     /locus_tag="OO464_002850"
     CDS             9671..11143
                     /gene="der"
                     /locus_tag="OO464_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6227931.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11213..11428
                     /locus_tag="OO464_002851"
     CDS             11213..11428
                     /locus_tag="OO464_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6227932.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11425..12795)
                     /gene="xseA"
                     /locus_tag="OO464_002852"
     CDS             complement(11425..12795)
                     /gene="xseA"
                     /locus_tag="OO464_002852"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6227933.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            12957..14423
                     /gene="guaB"
                     /locus_tag="OO464_002853"
     CDS             12957..14423
                     /gene="guaB"
                     /locus_tag="OO464_002853"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6227934.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14492..16069
                     /gene="guaA"
                     /locus_tag="OO464_002854"
     CDS             14492..16069
                     /gene="guaA"
                     /locus_tag="OO464_002854"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6227935.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16164..16703)
                     /locus_tag="OO464_002855"
     CDS             complement(16164..16703)
                     /locus_tag="OO464_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR6227936.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16719..17237)
                     /locus_tag="OO464_002856"
     CDS             complement(16719..17237)
                     /locus_tag="OO464_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6227937.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17548..17739)
                     /locus_tag="OO464_002857"
     CDS             complement(17548..17739)
                     /locus_tag="OO464_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR6227938.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17757..17909
                     /locus_tag="OO464_002858"
     CDS             17757..17909
                     /locus_tag="OO464_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6227939.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18091..>19224
                     /gene="pdeF"
                     /locus_tag="OO464_002859"
     CDS             18091..>19224
                     /gene="pdeF"
                     /locus_tag="OO464_002859"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6227940.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4528 a         4682 c         5225 g         4789 t
ORIGIN      
        1 gcgcagcatg gcaagatctc acttcgggac gggttatttc cggtggcagc acgctcacta
       61 tgcaggttgc tcgtctgctt gatcctcacc ccaaaacatt tggtggcaaa attctccagc
      121 tctggcgcgc gttgcaactg gagtggcatt tgtctaagcg tgaaattctg atcttgtatc
      181 ttaaccgcgc tccgtttggc ggtacgttgc aggggatcgg tgcggcaagt tgggcttatc
      241 tcggaaaatc gcctgcgaat ttaagctatt ccgaggcggc aatgctggcg gtgttaccgc
      301 aagtgcccag ccgactgcgc ccggatcgtt ggccggagcg tgccgaagcc gcgcgtaata
      361 aagtgctcga acggatggct gcgcaaggtg tgtggtcccg tgagcaggta aaagagtcaa
      421 gggaagaacc catctggctg gcaccccgac aaatgccgca actggcaccg ctgttttcgc
      481 gcatgatgct cggtaaaagc aaaagcgaca aaatcgttac tacgctggat gccggtcttc
      541 aacgacgtct ggaagaactg gcgcaaaact ggaaagggcg gttgccaccg cgcagctcac
      601 tggcgatgat cgtggttaat cataccgata tgcgtgttcg cggctgggtg ggatcggttg
      661 atctcaacga tgattcacgc tttggtcatg ttgatatggt caatgcgatc cgatcgccag
      721 gatcggtgct caaaccgttt gtttatggtc tggcgctgga tgaaggcttg atccacccgg
      781 catcactgct gcaagacgtc ccccggcgca ccggtgatta tcgaccaggt aactttgata
      841 gcggttttca tggcccgatc aacatgagcg aggcgctggt gcgctcgctg aacttacctg
      901 ctgtgcaggt gctggaagcc tatggaccga aacggtttgc ggcaaagtta cgcaatgttg
      961 gattgccgtt atatttgccc aacggcgctg cgccgaatct ttcactcatt ctcggcggcg
     1021 ctggtgcaaa actggaagat atggcggcag cgtataccgc gtttgctcgc cacggcaagg
     1081 caggcaaatt gcgcttacag cctgacgatc cgctgcttga acggccttta atgtcgtcgg
     1141 gggcggcgtg gatcattcgc cggattatgg ctgatgaagc gcaacccttg ccggatagtg
     1201 ccttgccgcg cgtcgcccca ctggcatgga aaacgggcac cagctatggc tatcgtgacg
     1261 cctgggcgat tggggttaac gctcgctatg tcattgggat ctggactggc agacctgacg
     1321 gcacgcccgt tgttggtcag tttggctttg ccagtgccgt accattgttg aatcaggtca
     1381 ataatatctt actgtcgcgc agtgcgaatc tgccggaaga cccgcgcccg aactcagtca
     1441 ctcgtggcgt tatctgctgg ccgggcggac agtctttgcc ggaaggtgac ggtaactgtc
     1501 gccgccgcct ggcaacctgg ctgctggatg gcagccagcc gccaacgctg ttactgtcag
     1561 agcaggaagg gattaatggc attcgcttcc ccatctggct ggatgacaaa gggcagcgcg
     1621 tcgccgctga ctgcccgcag gcgaagcaag aaatggtaaa tgtctggccg ctaccgctgg
     1681 aaccctggct gcccgcatca gaacgtcgcg ctgtgcgctt accaccggcc tcgacaattt
     1741 gcccgccata cggtcacgat gcccagctcc ctctgcagct gacaggcgtt cgcgatggcg
     1801 cgattattaa acgtttaccg ggcgcagcgg aagcgacttt gccgttgcaa tcaagtggag
     1861 gggcaggtga acgctggtgg tttctgaatg gcgaaccgtt aactgaacgc gggcgcaacg
     1921 tgaccctgca tttgacggat aaaggtgatt atcaattgct ggtgatggat gatgtggggc
     1981 aaatcgcgac agtgaaattt gtcatgcaat agtctgtttt gtccctgatt gttgctaaaa
     2041 ctcattttat tttaaaaaaa atgttacctt catcaatagt caacggccct gttgctcatt
     2101 ataatccgcg ccatctcgta cgctggtaca gacaacaaca gaacaattta cagaggtaaa
     2161 aatggctatt gaacgtactt tttccatcat caaaccgaac gcggtagcaa aaaacgtcat
     2221 tggtaatatc tttgcgcgct ttgaagctgc agggttcaaa attgtcggca ccaaaatgct
     2281 gcatctgacc gttgaacagg cacgtggctt ttatgctgaa cacgatggaa aaccgttctt
     2341 tgatggtctg gttgaattca tgacctctgg cccgatcgtg gtttccgtgc tggaaggtga
     2401 aaacgccgtt cagcgtcacc gcgatctgct gggcgcgacc aatccggcaa acgcactggc
     2461 tggtactctg cgcgctgatt acgctgacag cctgaccgaa aacggtaccc acggttctga
     2521 ttccgtcgaa tctgccgctc gcgaaatcgc ttatttcttc ggcgaaggcg aagtgtgccc
     2581 gcgcacccgt taataatttc gtaaatgccg cgtgcaaacg tggcatccgt gcgccagaat
     2641 ttgtacaatg cagcgccccc ggacgagcag ccgctcaccg gggcgtttct tttttcaacc
     2701 ctccaggggc cataacgtgt aataacgagg ccggaatagc atatgtctga acaattagtc
     2761 acacctgaaa acgtcaccac gaaagatgga aaaatcaacc tgctggatct caaccgtcag
     2821 cagatgcggg agttttttaa agatttaggt gaaaaaacct tccgcgccga tcaggtgatg
     2881 aagtggatgt atcactattg ctgcgacaac tttgatgaga tgaccgacat caacaaagtg
     2941 ttgcgcggca aactgaaaga ggtggcggaa atccgcgcac cggaagtggt tgaagaacag
     3001 cgttcatccg acggcaccat taaatgggcg atcgccgttg gcgatcagcg cgtcgaaacg
     3061 gtgtatatcc cggaagacga ccgtgccacg ctctgcgtct cttcgcaggt ggggtgtgcg
     3121 ctggagtgta aattctgttc caccgcccag cagggcttta accgcaacct gcgggtatcg
     3181 gaaattattg gccaggtgtg gcgtgcggcg aaaatcgtcg gcgcagcaaa agtcaccggt
     3241 cagcgcccaa tcactaacgt cgtgatgatg ggcatgggcg agccgctgct caacctgaac
     3301 aacgtcgttc cggcgatgga aatcatgctt gatgatttcg gttttggcct gtctaaacgt
     3361 cgcgtgacgc tttccacttc cggtgtagtt ccggcgctgg ataaactggg cgatatgatc
     3421 gacgttgcac tggcaatttc cctgcacgcg ccgaacgacg aaattcgtga cgaaattgtg
     3481 ccgatcaaca aaaagtacaa tatcgaaacg ttccttgctg cggtgcgccg ttatctggag
     3541 aaatccaacg ccaatcaggg ccgagtcact attgaatacg tgatgcttga ccacgttaac
     3601 gacggcactg aacacgcgca ccaactggcg gaactgctga aagatacgcc gtgtaagatc
     3661 aacctgatcc cgtggaaccc gttcccggac gcgccgtatg gacgcagttc gaacagccgt
     3721 atcgaccgtt tctcaaaagt gctgatgagc tacggtttca ccactattgt gcgtaaaacg
     3781 cgtggtgatg atatcgatgc tgcctgtggt cagttggcgg gcgatgttat cgaccgtacg
     3841 aaacgtactc tgcgtaaacg gatgcagggt gaagccatcg atattaaagc ggtctgatag
     3901 ccattacgcc acggttactt tatgtaccgt ggcgtaatct tttatgaatg aagtattaag
     3961 ttgcaattgt ctggtcaata ttaacggtgc gtttttgctg actttaaggc agtatgtaac
     4021 gatgcaacag taagttagcc tcagtgaatg tgggctttgt cacgagcaca cagacggtct
     4081 tatactgtat gataacggtt aacttaacgg atgtttcgcg gtgtgggtga gcatgatggt
     4141 tcaccggcat ctcaattctc atttaaacgt acctgcagcg aatgaatact gaagccacgc
     4201 acgaccaaaa tgaagcactt actaccggcg ctcgcctgcg taatgctcgc gaacaactag
     4261 gacttagtca gcaggccgtt gccgagcgac tttgcctgaa ggtttccacg gtacgcgaca
     4321 ttgaagaaga taaggcaccc gccgatcttg cttcaacatt cctgcgcgga tatatccgct
     4381 cttatgcgcg tctggtacat attccagaag aagaactgct gccagggctg gaaaagcagg
     4441 ctccacttcg ggctgcaaaa gttgcgccaa tgcagagttt ttccctcggt aaacgccgca
     4501 aaaaacgcga cggctggcta atgaccttca cctggctggt gttgtttgtg gttatcggcc
     4561 tgagcggtgc ctggtggtgg caagaccaca aagctcagca ggaagagatc accactatgg
     4621 ccgatcaatc ttcggcggaa ctgagcagta atagcgagca ggggcagagt gttccgttaa
     4681 atacgtcgac aactacagac ccggctacaa cgtccacgcc gccagcgtct gtggatacta
     4741 ccgcaaccaa cacgcaaaca cctgccgtaa ctgcgccagc accagctgtt gatccgcaac
     4801 agaatgcggt tgtttcgcct tcgcaggcaa atgttgatac cgccgcgacc ccggtaccaa
     4861 cggcagcaac aacgccagat ggtgctgcgc cgttgccaac cgatcaggct ggcgtgacca
     4921 cgccggcggc tgatccgaat gcgctggtga tgaactttac tgccgattgc tggctggagg
     4981 tcactgatgc taccggtaaa aaattgttta gcgggatgca gcgtaaagac ggtaatttga
     5041 atttaaccgg ccaggcaccg tataaactga aaattggtgc gccagccgca gtacagatcc
     5101 agtatcaagg gaaacctgtc gatctgagtc gttttatcag aactaaccag gttgcgcgtc
     5161 tgaccctcaa tgccgaacaa tcaccggcgc agtaacagac gggtaacgcg ggagattttt
     5221 catgcataac caggctccaa ttcaacgtag aaaatcaaca cgtatttacg ttgggaatgt
     5281 gccgattggc gatggtgctc ccatcgccgt acagtccatg accaatacgc gtacgacaga
     5341 tgtcgaagca acggtcaatc aaatcaaggc gctggaacgc gttggcgctg atatcgtccg
     5401 tgtctccgta ccgacgatgg acgcggcaga agcgttcaaa ctcatcaaac agcgggttaa
     5461 cgtgccgctg gtggctgaca tccacttcga ctatcgcatt gcgctgaaag tagcggaata
     5521 cggcgtcgat tgtctgcgta ttaaccctgg taatatcggt aatgaagagc gtattcgcat
     5581 ggtggttgac tgtgcgcgcg ataaaaacat tccgatccgt attggcgtta acgccggatc
     5641 gctggaaaaa gatctgcaag aaaagtatgg cgaaccgacg ccgcaggcgt tgctggaatc
     5701 cgccatgcgt catgttgatc atctcgatcg cctgaacttc gatcagttca aagtcagcgt
     5761 gaaagcgtct gacgtcttcc tcgctgttga gtcttatcgt ttgctggcaa aacagatcga
     5821 tcagccgctg catctgggga tcaccgaagc cggtggtgca cgcagcgggg cagtaaaatc
     5881 cgccattggt ttaggtctgc tgctgtcgga aggcatcggc gacacgctgc gtgtatcgct
     5941 ggcggccgat ccggtcgaag agatcaaagt cggtttcgat attttgaaat cgctgcgtat
     6001 ccgttcgcga gggattaact tcatcgcctg cccgacctgt tcgcgtcagg aatttgatgt
     6061 tatcggtacg gttaacgcgc tggagcaacg cctggaagat atcatcactc cgatggacgt
     6121 ttcgattatc ggctgcgtgg tgaatggccc aggtgaggcg ctggtttcta cactcggcgt
     6181 caccggcggc aacaagaaaa gcggcctcta tgaagatggc gtgcgcaaag accgtctgga
     6241 caacaacgat atgatcgacc agctggaagc acgcattcgt gcgaaagcca gtcagctgga
     6301 cgaagcgcgt cgaattgacg ttcagcaggt tggaaaaata ataacgtgat gggaagcgcc
     6361 tcgcttcccg tgtatgattg aacccgcatg gctcccgaaa cattgaggga agcgttgagg
     6421 gttcattttt atattcagaa agagaataaa cgtggcaaaa aacattcaag ccattcgcgg
     6481 catgaacgat tacctgcctg gcgaaacggc catctggcag cgcattgaag gcacactgaa
     6541 aaacgtgctc ggcagctacg gttacagtga aatccgcttg ccgattgtag agcagacccc
     6601 gctattcaaa cgtgcgattg gtgaagtcac cgacgtggtt gaaaaagaga tgtacacctt
     6661 tgaggatcgc aatggcgaca gcctgactct gcgccctgaa gggacggcgg gctgtgtacg
     6721 cgccggcatc gagcatggtc ttctgtacaa tcaggaacag cgtctgtggt atatcgggcc
     6781 gatgttccgt cacgagcgtc cgcagaaagg gcgttatcgt cagttccatc agttgggctg
     6841 cgaagttttc ggtctgcaag gtccggatat cgacgctgaa ctgattatgc tcactgcccg
     6901 ctggtggcgt gcgctgggta tcttcgaaca cgtaactctt gagctgaatt ctatcggttc
     6961 gctggaagca cgcgccaatt accgcgatgc gctggtggca ttccttgagc agcataaaga
     7021 aaagctggac gaagactgca aacgccgcat gtacactaac ccgctgcgcg tgctggattc
     7081 aaaaaatccg gaagtgcagg cgcttctcaa cgacgctccg gcattaggtg attatctgga
     7141 cgaggaatct cgtgagcatt ttgccggtct gtgcaaactg ctggagagcg cggggatcgc
     7201 ttacaccgta aaccagcgtc tggtgcgtgg tctggattac tataaccgta ccgttttcga
     7261 gtgggtgact aacagtctcg gctcccaggg caccgtgtgt gcaggcggtc gttatgacgg
     7321 tcttgtggaa caactgggcg gtcgtgcaac accggctgtc ggttttgcga tgggcctcga
     7381 acgtcttgta ttgttagtac aggccgttaa tccggaattt aaagccgatc ctgttgtcga
     7441 tatatacctg gtggcttcag gtgctgatac acaatctgcg gctatggcat tagctgagcg
     7501 tctgcgtgat gaattactgg gcgtgaaatt gatgaccaac cacggcggcg gcaactttaa
     7561 gaaacagttt gcccgtgctg ataaatgggg tgcccgcgtt gctgtggtgc tgggtgagtc
     7621 tgaagtggct aacggcacag cagtagtgaa ggatttgcgc tctggtgagc aaacggcagt
     7681 tgcgcaggat agcgtagccg cgcatttgcg cacgttactg ggttaaggaa ggagaaggac
     7741 agcgtggaaa tttacgagaa cgaaaacgac caggtagaag cggttaaacg cttttttgct
     7801 gaaaatggca aagcactggc tgttgggggg attttgggcg ttggcgcact gactggctgg
     7861 cgctactgga acagccatca ggttgattct gcacgctccg cttctcttgc ctatcaaaat
     7921 gcggttaccg cagtgagcga aggcaaaccg gatagcatcc cggcggcgga aaaatttgct
     7981 gctgaaaata aaaatactta tggtgcgctg gcttctttgg aacttgcgca gcaatttgtt
     8041 gacaaaaatg aactagagaa agctgccgcc cagttacaac aggggctggc agacacgagc
     8101 gatgaaaatc tcaaagccgt gataaatctg cgtcttgctc gcgttcaggt acagctcaag
     8161 caggctgatg ccgcgctgaa aacccttgat accatcaaag gtgaagggtg ggctgccatt
     8221 gttgccgact tgcgtggtga agcattgctg agcaaaggtg ataagcaagg tgcgcgtagt
     8281 gcatgggaag caggcgtgaa aagcgatgtt actccggcac tgagcgaaat gatgcagatg
     8341 aaaattaata atttgtccat ctgagaggga cccgatgcaa ttgcgtaaat tactgctgcc
     8401 aggactgctt tccgttaccc ttttaagcgg ctgttcgctg tttaacagcg aagaagatgt
     8461 ggtaaagatg tccccattgc caaccgttga aaaccagttt acgccgacca cggcgtggag
     8521 cacttccgtt ggtagcggca ttggcaactt ctattccaat cttcatccgg cactggcgga
     8581 caacgttgtc tatgcagcgg accgcgctgg tttggtaaaa gcgctgaatg cggatgatgg
     8641 caaagaaatc tggtctgtca gcctggccga gaaagatggc tggttctcta aagagcctgc
     8701 attactttct ggcggtgtga ccgtgtctgg tgggcatgtc tacattggca gcgaaaaggc
     8761 gcaggtttac gcgctgaata ccagcgatgg tactgtggca tggcaaacta aagtcgcggg
     8821 tgaagcactt tcgcgcccgg tggtcagcga cggtctggtg ttaatccaca ccagtaacgg
     8881 tcagttacaa gcgctgaacg aagctgacgg cgctgtcaaa tggacagtta acctcgatat
     8941 gccttcgctc tctttgcgtg gcgagtctgc gccggcaacg gcttttggtg cggccgtcgt
     9001 gggtggcgat aatggtcgcg tcagcgcagt gctgatggaa cagggccaga tgatttggca
     9061 gcagcgtatt tcccaggcga ccggttctac cgaaattgac cgtctgagcg atgttgacac
     9121 gactcccgtc gttgttaacg gcgttgtttt cgcgctggcc tataatggta acctgacggc
     9181 gcttgatctg cgcagtggtc agattatgtg gaaacgcgaa ctgggttcgg tgaatgattt
     9241 catcgtcgat ggcaatcgca tctatctggt cgatcaaaat gaccgggtga tggcgttgac
     9301 cattgatggc ggcgttacgc tgtgggcaca aagcgatctg ctgcatcgcc tgctgacttc
     9361 tccggtgctg tataatggca acctggtggt cggtgacagt gaaggttatc tgcactggat
     9421 taacgtcgaa gatggtcgtt tcgttgccca gcaaaaagtt gatagttccg gtttccagac
     9481 tgaaccggtt gccgctgacg gcaaactgct gatccaggca aaagacggaa ccgtgtactc
     9541 tattacacgt taatcgtctc tgtcgttcac tttgaaaacg gctcctggac aggggccgtt
     9601 ttcctgtttt taacaacgac gcgaatatag cgtgcgttgt ctgatgattt ataaaaatga
     9661 ggctttaaac atggtacctg tggtcgcgct tgtcgggcgc cctaacgtag gaaaatccac
     9721 gttatttaac cgtctaactc gcacccgaga tgcgctggtt gcggatttcc cgggtctgac
     9781 tcgtgaccgt aagtacggtc gtgcggaaat tgaaggccgt gagtttatct gtattgatac
     9841 cggcgggatt gatggcacag aagacggtgt agaaacccgc atggcggaac agtcgctgct
     9901 ggcgattgaa gaagcggacg tcgtactgtt tatggtggat gcgcgcgcgg gcctgatgcc
     9961 ggcagatgaa gcgattgcca aacatctgcg ctcccgtgaa aaaccgacct tcctggtggt
    10021 aaacaaaact gacggtctgg atcccgatca ggcagtggtt gatttctact cgcttggttt
    10081 aggtgaaatc tacccgatcg ccgcgtctca cggtcgtggc gtattaagtc tgctggagca
    10141 tgtgctgctg ccgtggatgg aagatctcgc accgcaagag gaagtcgacg aagacgctga
    10201 atactgggcg caatttgaag cggaagagaa cggcgaagaa gaagaggaag acgacttcga
    10261 cccgcaaagt ctgccgatca aactggcgat tgtgggtcgt ccgaacgtag gtaagtctac
    10321 actcactaac cgtattcttg gagaagagcg cgttgttgtt tacgacatgc ctggcacgac
    10381 gcgtgacagc atctacatcc caatggaacg cgatggacgt gagtatgtgc tcattgacac
    10441 cgctggcgta cgtaaacgcg gcaaaatcac cgatgctgta gagaaattct ccgtaatcaa
    10501 aacgttgcag gccattgaag acgccaacgt ggtgatgtta gtgattgatg cgcgcgaagg
    10561 tatttccgat caggatctct cgctgctggg ctttattctc aatagtgggc gctcacttgt
    10621 cattgtggtg aataagtggg atggcctgag ccaggaagtg aaagagcagg tgaaagaaac
    10681 gctggacttc cgtctgggct ttatcgattt tgctcgtgtg cactttatct ctgccttgca
    10741 cggcagtggt gttggtaact tgtttgaatc agtacgtgaa gcgtatgaca gctccacccg
    10801 tcgtgtgggg acctctatgc tgacgcgcat catgacgatg gctgttgaag atcaccaacc
    10861 gccgctggta cgcggtcgtc gtgtgaagct gaaatatgcc cacgctggtg gttataaccc
    10921 gccgattgtg gtgattcacg gtaatcaggt gaaagacctg cctgattcct acaagcgcta
    10981 cttgatgaac tacttccgca aatcgctgga cgtaatggga tcgccgattc gtattcagtt
    11041 caaagaaggg gaaaacccgt atgcgaacaa gcgtaacacc ctgacgccaa cccagatgcg
    11101 taaacgtaag cgtctgatga agcacatcaa gaaaaataaa taattatccg taagtatttt
    11161 atctgccatt ccctctatga atgtagaggg aatggctacc ggaggaattc atatggaact
    11221 gcattgtcca aaatgtcagc atgtgcttga tcaggataac ggccatgcgc gttgcccatc
    11281 ctgcggggaa tttattgaaa tgaaagctct ttgtccagac tgccatcaac cgttgcaggt
    11341 gttaaaagcc tgcggtgcag tagattactt ctgtcagcac gggcatggct tgatatcgaa
    11401 aaaacgcgtt gaattcgtgc tggcttaatg aaccttttta cgcgattttt ttaccggctg
    11461 gatgtttttt acttcacttt ctatccagcc gtcttccaga cgtgtggtta gcatttcacc
    11521 cgttttaact tgcttcactt ttttcagtac attgccgtca gtagcagtag taacgctgta
    11581 tccacgcgcc agcgttgaca gtgggcttac ggcttcgagg tgcgttactg cattaccgaa
    11641 acgttcacgc gtggcgctaa gctgtgcgcg caggatttct gctaaacgat attccagttg
    11701 ctgaatgcgc gtttgcgtgc gatgaatctt cggctgtgga ttttgctgat tcagccgctg
    11761 tgttaatcgc tgctgctgtt gcccggcacg cttaagctga ttttccagcg caaagctcat
    11821 ccgtttttgc aggcgttcaa gcatggtttg ctggcgtgcc agccggagct gcggatgctg
    11881 ctgctgcaag cgatgatgaa tctgcgtaaa gcgacgtgta cggttggcga gataataatc
    11941 catcgccatc tccagccgtt gacgggccga ttgcacctgg cgcagtaact cttgctgatt
    12001 acggctcact acttcagcgg cggcagacgg cgttggcgca cgcagatcgg caacaaaatc
    12061 ggcaatggtc acatccgtct catgcccgac ggcgctgacg accggaatgc ggctggcaaa
    12121 aatcgcccgc gctacgcgtt cgtcgttaaa actccataaa tcttccagcg aaccgccgcc
    12181 gcgcccaacg atcaacacgt cgcactcatt gcgctgattc gccagttcaa tggcgcgaac
    12241 aatttgcccc ggcgcgtcat cgccctgaac ggaggtgggg tagatgatca ccggtagaga
    12301 aggatcgcga cgttttaaca catgcaaaat atcatgtagc gcagcaccgg tttttgaggt
    12361 gatcacacca acgcaatgcg caggggaggg aagtggtttt ttgtattgct gatcgaacaa
    12421 acattcagcc tgcaacttcg ctttgagctg ttcgtacttc tgttgcagca gcccttcacc
    12481 ggccggctgc atactctcaa cgattatctg gtagtcgccg cgcggctcgt agagcgtaat
    12541 attggcgcga actaaaactt gttgcccatg ctgcggacgg aaggtgaccc ggcggttgct
    12601 gttgcggaac atcgcgcagc gtacctgggc ggtgtcgtct ttgagtgtaa agtaccagtg
    12661 accggaagcc ggttgtgtga aattagagat ttcgccgctg atccaaacct gtcccatctc
    12721 atgctcaagc agcagacgaa ccgtttgatt caggcgacta acggtaaaaa ttgcagggga
    12781 ttgagaaggt aacatgtgag cgagatcaaa ttctaaatca gcaggttatt cagtctatag
    12841 taacccgccc ttcggggata gcaagcattt tttgcaaaaa ggggtagatg caatcggtta
    12901 cgctctgtat aatgccgcgg caatatttat taaccactct ggtcgagata ttgcccatgc
    12961 tacgtatcgc taaagaagct ctgacgtttg acgacgttct cctcgttcct gctcattcta
    13021 ccgttctgcc gaatactgct gacctcagca cccagctgac gaaaactatt cgtctgaata
    13081 tccctatgct ttccgcagca atggataccg taacggaagc gcgcctggct attgctctgg
    13141 ctcaggaagg cggtatcggc tttatccaca aaaacatgtc cattgaacgc caggcagaag
    13201 aagttcgccg tgtgaaaaaa cacgaatctg gtgtggtgac tgatccgcag actgttctgc
    13261 caaccacgac gctgcgcgaa gtgaaagaac tgaccgagcg taacggtttt gcgggctatc
    13321 cggtcgttac cgaagaaaac gaactggtgg gtattatcac cggtcgtgac gtgcgttttg
    13381 ttaccgacct gaaccagccg gttagcgttt acatgacgcc gaaagagcgt ctggtcaccg
    13441 tgcgtgaagg tgaagcccgt gaagtggtgc tggcaaaaat gcacgaaaaa cgcgttgaaa
    13501 aagcgctggt ggttgatgac gaattccacc tgatcggcat gatcaccgtg aaagacttcc
    13561 agaaagcgga acgtaaaccg aacgcttgta aagacgagca aggccgtctg cgtgttggtg
    13621 ctgcagttgg cgcaggtgcg ggtaacgaag agcgtgttga cgcgctggtt gccgctggcg
    13681 ttgacgttct gctgatcgac tcctcccacg gtcactcaga aggcgttttg cagcgtatcc
    13741 gtgaaacccg tgctaaatat ccggatctgc aaatcatcgg cggcaacgtg gcaacagctg
    13801 caggtgcacg cgctttggca gaagctggtt gcagcgcggt taaagtcggt atcggccctg
    13861 gttctatctg tacgactcgt atcgttactg gcgtaggtgt tccgcagatc actgccgttg
    13921 ctgacgcagt tgaagccctg gaaggcaccg gtattccggt tatcgcagat ggtggtattc
    13981 gcttctccgg cgacatcgcc aaagctatcg ccgctggcgc aagcgcggtg atggtaggtt
    14041 ccatgctggc gggtactgaa gaatctccgg gtgaaatcga actctaccag ggccgttctt
    14101 ataaatctta ccgtggtatg ggttccctgg gcgcgatgtc caaaggttcc tctgaccgtt
    14161 atttccagag cgataacgct gccgacaaac tggtgccgga aggtatcgaa ggtcgcgtag
    14221 cttataaagg tcgcctgaaa gagatcattc accagcagat gggcggcctg cgctcctgta
    14281 tgggtctgac cggctgtggt actatcgacg aactgcgtac taaagcggag tttgtacgta
    14341 tcagcggtgc gggcattcag gaaagccacg ttcacgacgt gaccattact aaagagtccc
    14401 cgaactaccg tctgggctcc tgattctctt cgcccgattt catgtcgggc gatttatatt
    14461 atctgtttca cttgcctcgg aataagcgtc aatgacggaa aacattcata agcatcgcat
    14521 cctcattctg gacttcggtt ctcagtacac tcaactggtt gcgcgccgcg tgcgtgagct
    14581 gggtgtttac tgcgaactgt gggcgtggga tgtgacagaa gcacaaattc gtgacttcaa
    14641 tccaagcgga attattcttt ccggcggccc ggaaagtact actgaagaaa acagtccgcg
    14701 tgcgccgcag tatgtctttg aagcaggcgt accgatattc ggcgtttgct atggcatgca
    14761 gaccatggca atgcagttgg gcggtcacgt tgaagcctct aacgaacgtg aatttggcta
    14821 cgcgcaggta gaagtcgtaa acgacagcgc actggttcgc ggtatcgaag atgcgctgac
    14881 cgcagacggt aaaccgctgc tcgatgtctg gatgagccac ggcgataaag ttaccgctat
    14941 cccgtccgac ttcgtcaccg tagccagcac cgaaagctgc ccgtttgcca ttatggctaa
    15001 cgaagaaaaa cgcttctatg gcgtacagtt ccacccggaa gtgactcaca cccgccaggg
    15061 tatgcgcatg ctggagcgtt ttgtgcgtga tatctgccag tgtgaagccc tgtggacgcc
    15121 agcgaaaatt atcgacgatg ctgtagctcg catccgcgag caggtaggcg acgataaagt
    15181 catcctcggc ctctctggtg gtgtggattc ctccgtaacc gcaatgctgc tgcaccgtgc
    15241 tatcggtaaa aacctgactt gcgtattcgt cgacaacggc ctgctgcgtc tcaacgaagc
    15301 agagcaggtt ctggatatgt ttggcgatca ctttggtctt aacattgttc acgtaccggc
    15361 agaagatcgc ttcctgtcag cgctggctgg cgaaaacgat ccggaagcaa aacgtaaaat
    15421 catcgggcgc gttttcgttg aagtattcga tgaagaagcg ctgaaactgg aagacgtgaa
    15481 gtggctggcg cagggcacca tctaccctga cgttatcgaa tctgcggctt ctgcaaccgg
    15541 taaagcacac gtcatcaaat ctcaccacaa cgtgggcggc ctgccgaaag agatgaagat
    15601 gggcctggtt gaaccgctga aagagctgtt caaagacgaa gtgcgtaaga ttggtctgga
    15661 gctgggcctg ccgtacgaca tgctgtaccg tcatccgttc ccgggaccag gccttggcgt
    15721 tcgtgttctg ggtgaagtga agaaagagta ctgtgacctg ctgcgccgtg ctgacgccat
    15781 cttcattgaa gaactgcgta aagcggacct gtacgacaaa gtcagccagg cgttcaccgt
    15841 cttcctgcca gtacgttccg ttggcgtaat gggcgatggt cgtaagtatg actgggttgt
    15901 ctctctgcgt gctgtcgaaa ccatcgactt tatgaccgca cactgggcgc atctgccgta
    15961 cgatttcctc ggtcgcgttt ccaaccgcat tatcaatgaa gtgaacggta tttcccgcgt
    16021 ggtgtatgac atcagcggca agccgccagc taccattgag tgggaatgat tttgatccct
    16081 gcactatgaa tgaacaaaac cctccgttac tacagagggt tttttatctt caagaatcat
    16141 aggcttgaag ttactaacat cgattaatta aaccagctgt ccgatttgtt ctcttctgct
    16201 ttgcccacgc ttttcatcag atcgcgaccg ccttcagtca tatttctgtt tgcgtcagct
    16261 tcagattgca ccacatcggt ttgcgcagct ttgtgcttca gttcctgatc gataaattcg
    16321 ttttcgcgct taacgcgggc ttcttctttc gccagcgcca gtttttgttt ctgaatctct
    16381 aagctgcgta gctcatcttc ataactttga tcgcgttttt tgtccgcagt ggcttcggcg
    16441 tccagtttat cctgacgagc tttcttattt gccgctgccg ttgccgctct tttgttcgcg
    16501 gcggcctggg catttgcgcg acgttgcttc tcttgctgga tttccctgtt gcgctccgcg
    16561 acccattcgt catgctgcct ttgctcttca tttttacctt gctgttccgc ttctgcgaca
    16621 gcagagagtt gatcctgcaa tgatgaggct atagccggat agcttaagga ggctaagatg
    16681 gcgcaaagaa aaactttctt catgactcct cctgattatt agctcttttc aggacatttg
    16741 gtatttggct gaatacgcgt ttcgttatac gttgtggtaa taacaacggc taaacctgtc
    16801 gtaaactggc actctttacc ctcctgggtg gaggtataca ctttggtgcc ttccttatat
    16861 gttaaagaaa caccttccac taaggtttta tcattcacca tagaacccgc tgccgcgcct
    16921 acagctccgc cgccaactgc ccctgccgtc gttccggaat tgctgccaga accgacgttg
    16981 tggccgataa caccgccagc gactgcgcca ataagcgcgc cgaaggcttg tgcgttccgt
    17041 ttattttggg agttgtctac ggcaactttt gcgggaagaa tggaaataat attaacggtt
    17101 ttagtttctt gtttggtatt cagttgatcg gtttgataaa catcggcggc atgatcgtca
    17161 gcatttgact ggcatcctgc cagagtgaat gacgctaaca ttgccacagg cagaagacat
    17221 tttttaaatt tcatcactat tccttgttat acacattacg aaaaataaga catttaagat
    17281 catcaaaaga ggaaagtaaa tgtattgcgt ataaagggat agattaaatt tattaaaatt
    17341 cacgcaggga ataatttata aaaatatatc attacaacat atatattaat tttatattta
    17401 tttcgcagcc aaacaggata aacatcagtc aggacaaaga cgtaaaattt aagatgaaag
    17461 aatatctgcc gctttgataa tcccaaagag caaaagcatc cgacagcgaa gtgccgttat
    17521 gttctgtgaa agtggagaag gtaaccgcta acgttgtacc ggtgattcga cggagagtgt
    17581 ggattgctga gcttcttttt tgctctgatg gtgctgccag gcaccgacgg acgagtagat
    17641 aaaacggcca aagaagaaga taaagctgat gagcagtacg atacgggtca tgcgactgtt
    17701 aaatcggtgt cgttttcgca tactagttgc ctgactcaca aaaggttcct tgaagtatgt
    17761 cccacgctgt ggacggtact tacattaagg cacaacagga caaaatggtc aattcttcgt
    17821 tatgtaaaaa agcgtcagtg gatacatatt ttaatgttat ggaagttaat ttaattattt
    17881 acaatgatga tgtaataatg atgaattaac atgataaacg taataattca ttaatcaggg
    17941 ttattttgtt tgatatatat caattggaac tttcatatga cacttagaat caatgctctc
    18001 tatagtgatg aataatcatc attcgaagtc aggtgggatg cctgtctgaa tacacctcct
    18061 tcaggatgtg ggggattctg ctgagcatct atgaaactga atgcaactta tataaaaata
    18121 cgtgataaat ggtgggggct tccgctgttc ctgccttctt taatcttgcc cattttcgcc
    18181 cacattaata ctttcgcgca tatttcttcc ggtgaggttt ttctctttta tctgcctttg
    18241 gcactgatga tcagcatgat gatgtttttc agctgggcgg cattgccagg gatcacctta
    18301 gggatttttg tccgcaaata tgcagagctg ggtttttacg aaacgctctc attaacggct
    18361 aattttatta tcattatcat tctctgttgg ggcggttaca gggtctttac tccccggcgt
    18421 aacaacgttt cacatggtga tacccgttta atttcccagc gtatattctg gcagatagtg
    18481 tttcctgcaa cgctgtttct gatacttttc cagtttgctg catttgtagg attactggcg
    18541 agcagagaaa atctggtcgg tgtcatgcct tttaacctcg ggactttaat caattatcag
    18601 gccttactgg tgggtaatct gatcggtgtc ccgctgtgct acttcatcat tcgggtagtg
    18661 cgaaatccat tttatttacg tagctattat tcgcaattaa aacagcaggt tgatgccaaa
    18721 gtcaccaaaa aagagttcgc gctctggcta ctggcattag gtgctttatt gttgctgtta
    18781 tgcatgccgt taaatgaaaa aagcacaatt tttagcacca attatacctt gtcattattg
    18841 ctgcccctga tgatgtgggg agcaatgcgc tatggttata agctgatttc gctgctctgg
    18901 gcggtcgtgt tgatgatcag catccacagc tatcaaaatt acattcccat ttatcctggc
    18961 tataccacgc agctgaccat aacctcctcc agttatctgg tattctcttt tattgtcaat
    19021 tatatggctg tactggcaac tcgtcagcga gcggtagtca gacgcattca gcggcttgcg
    19081 tatgtggacc cggtggttca tctgccaaat gttcgcgccc tgaatcgcgc gttgcgtgat
    19141 gccccctggt ctgcgctttg ttatttacgc atccctggca tggaaatgct ggttaagaac
    19201 tatggcatca tgctgcggat tcaa
//
LOCUS       DAJTUI010000076        18793 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTUI010000076 DAJTUI010000000
VERSION     DAJTUI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.076"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(139..3654)
                     /locus_tag="OO464_002860"
     CDS             complement(139..3654)
                     /locus_tag="OO464_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6227941.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="OO464_002861"
     CDS             complement(4107..5369)
                     /locus_tag="OO464_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6227942.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="OO464_002862"
     tRNA            complement(5567..5642)
                     /locus_tag="OO464_002862"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="OO464_002863"
     CDS             complement(5748..6455)
                     /locus_tag="OO464_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6227943.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="OO464_002864"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="OO464_002864"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6227944.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO464_002865"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO464_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6227945.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO464_002866"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO464_002866"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6227946.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="OO464_002867"
     CDS             complement(11639..11914)
                     /locus_tag="OO464_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6227947.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO464_002868"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO464_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6227948.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="OO464_002869"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="OO464_002869"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6227949.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKVQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="OO464_002870"
     CDS             13874..14200
                     /locus_tag="OO464_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6227950.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="OO464_002871"
     CDS             14384..15103
                     /locus_tag="OO464_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6227951.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="OO464_002872"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="OO464_002872"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6227952.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="OO464_002873"
     CDS             16442..17404
                     /locus_tag="OO464_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6227953.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18793)
                     /locus_tag="OO464_002874"
     CDS             complement(17505..>18793)
                     /locus_tag="OO464_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR6227954.1"
                     /translation="AITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAAITVS
                     QRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNVLANM
                     MFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVLIVFS
                     LTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAFLDAL
                     PSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILESVVTT
                     SIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIFMDMT
                     PAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGVKIQH
                     LIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4895 a         4582 c         4643 g         4673 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa gtccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    18481 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    18541 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    18601 aagatgaaaa aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc
    18661 gaaacagtga ttgccgcgat atcccaggga aacatcaaaa gcattgaagc tacagtggca
    18721 ataccgattg agaacgaaat gggcacacca ataaagacca gcacaaaaaa acagccaaac
    18781 agtgtaatcg cga
//
LOCUS       DAJTUI010000077        18070 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTUI010000077 DAJTUI010000000
VERSION     DAJTUI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.077"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            258..782
                     /locus_tag="OO464_002875"
     CDS             258..782
                     /locus_tag="OO464_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6227955.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            863..1537
                     /locus_tag="OO464_002876"
     CDS             863..1537
                     /locus_tag="OO464_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6227956.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1553..4015
                     /locus_tag="OO464_002877"
     CDS             1553..4015
                     /locus_tag="OO464_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6227957.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            4031..5064
                     /locus_tag="OO464_002878"
                     /pseudo
     CDS             4031..5064
                     /locus_tag="OO464_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5146..5484)
                     /gene="rcnB"
                     /locus_tag="OO464_002879"
     CDS             complement(5146..5484)
                     /gene="rcnB"
                     /locus_tag="OO464_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR6227958.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5703..6533)
                     /gene="rcnA"
                     /locus_tag="OO464_002880"
                     /pseudo
     CDS             complement(5703..6533)
                     /gene="rcnA"
                     /locus_tag="OO464_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6655..6927
                     /gene="rcnR"
                     /locus_tag="OO464_002881"
     CDS             6655..6927
                     /gene="rcnR"
                     /locus_tag="OO464_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR6227959.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      7019..7115
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7150..7938
                     /gene="thiM"
                     /locus_tag="OO464_002882"
     CDS             7150..7938
                     /gene="thiM"
                     /locus_tag="OO464_002882"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6227960.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7935..8735
                     /gene="thiD"
                     /locus_tag="OO464_002883"
     CDS             7935..8735
                     /gene="thiD"
                     /locus_tag="OO464_002883"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6227961.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8800..9621
                     /locus_tag="OO464_002884"
     CDS             8800..9621
                     /locus_tag="OO464_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6227962.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9669..10415
                     /locus_tag="OO464_002885"
     CDS             9669..10415
                     /locus_tag="OO464_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6227963.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10389..11354)
                     /locus_tag="OO464_002886"
     CDS             complement(10389..11354)
                     /locus_tag="OO464_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR6227964.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11351..12354)
                     /locus_tag="OO464_002887"
                     /pseudo
     CDS             complement(11351..12354)
                     /locus_tag="OO464_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12394..13446
                     /gene="fbaB"
                     /locus_tag="OO464_002888"
     CDS             12394..13446
                     /gene="fbaB"
                     /locus_tag="OO464_002888"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6227965.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13754..14608
                     /gene="gatY"
                     /locus_tag="OO464_002889"
     CDS             13754..14608
                     /gene="gatY"
                     /locus_tag="OO464_002889"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6227966.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14637..15899
                     /gene="gatZ"
                     /locus_tag="OO464_002890"
     CDS             14637..15899
                     /gene="gatZ"
                     /locus_tag="OO464_002890"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR6227967.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15909..16361
                     /gene="gatA"
                     /locus_tag="OO464_002891"
     CDS             15909..16361
                     /gene="gatA"
                     /locus_tag="OO464_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6227968.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16392..16676
                     /gene="gatB"
                     /locus_tag="OO464_002892"
     CDS             16392..16676
                     /gene="gatB"
                     /locus_tag="OO464_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6227969.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16680..18035
                     /locus_tag="OO464_002893"
     CDS             16680..18035
                     /locus_tag="OO464_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR6227970.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4728 a         4112 c         4572 g         4658 t
ORIGIN      
        1 gaaatcattt atataaatga tttcgtcttt tttattgata tcaacaatac catttacata
       61 aatctacatt aattaataga tgtgataaat atttaatatt cttaagtatt tgttgtattt
      121 atattgcatt ggttaatttg ttttaaatca ataaaacaat atgtttacat tgcggcagct
      181 tttttgatgt gagaaatttg tttccaagag atacagactc ttaacaacca aaaataaatt
      241 ttattaagga acatattatg aaacgttcaa ttattgctgc cgctgtcttt tcttcttttt
      301 ttatgagcgc tggagtattt gctgcagacg ttgataccgg aacattaacc attaaaggaa
      361 atattgcaga atctccgtgt aaattcgaag cgggtggtga ttcagtaagt attaatatgc
      421 cgactgtacc aaccactgtc tttgaaggta aagctaaata ttctacctat gatgatgcag
      481 tcggtgtaac cagcagcatg ttaaaaatta gctgcccgaa agaagttgct ggtgtaaaac
      541 tctcgttgat cacaaacgac aaaataaccg gtaacgataa ggcgatagcc agtagcaacg
      601 ataccgtagg tgataacagc gatgtcctag atgtttctgc accttttaac attgagagtt
      661 ataaaacagc ggaaggtcaa tatgctattc cgtttaaagc aaaatacctg aaactgacag
      721 ataactcagt gcaatcaggt gatgtgttat cttctctggt tatgcgtgtg gcgcaggatt
      781 aataaataaa attgcaagtt tctccaggta ataaacatgg ccgctatccc catggcggct
      841 ttttaattta agaggcatta agatgaaagg attattatct ttactcattt tttctatggt
      901 ccttcctgca catgccggaa ttgttatcta cgggacgcgc attatttacc cggcagaaaa
      961 taaagaagtt atggtgcagt tgatgaacca gggaaatcgt tcttcgctgc tgcaggcgtg
     1021 gattgatgat ggcgatacgt cgttaccacc agaaaaaatt caggttcctt tcatgttaac
     1081 gccaccagtg gcaaaaatag gggcaaattc cgggcagcaa gtaaaaatca aaattatgcc
     1141 gaataaactg cccactaata aagaaagcat tttttatctg aatgttctgt atattccacc
     1201 aaatagtcca gagcaagaag gcaagaatgc actgaagttt gcgatgcaaa acagaattaa
     1261 gttgttttac cggccagcgg gtattgctcc ggtaaataaa gcgaccttta aaaagttact
     1321 ggtaaatcgc agtggcaatg gattggtgat aaaaaatgac tcagctaatt gggtgacgat
     1381 ttcggatgtc aaagctaata atgttaaagt caattatgaa actattatga ttgccccctt
     1441 agaaagtcag agtgttaata tcaaaagtaa taatgcaaat aactggtatc tgaccattat
     1501 caatgaccac ggcaactata ttagtgacaa aatttaattg caggagctgc ctatgttgag
     1561 aatgacccca cttgcatcag caatcgtagc gttattgctc ggcattgaag cttatgcagc
     1621 tgaagaaacc tttgataccc attttatgat aggtggaatg aaagaccagc aggttgcaaa
     1681 tattcgtctt gatgataatc aacccttacc ggggcagtat gacatcgata tttatgtcaa
     1741 taagcaatgg cgcgggaaat atgagattat tgttaaagac aacccgcaag aaacatgttt
     1801 atcaagagaa gttatcaagc ggttaggcat taatagcgat aacttcgcca gcggtaagca
     1861 atgtttaaca tttgagcaac ttgttcaggg tgggagctat agctgggata tcggggtttt
     1921 tcgtctcgat ttcagtgtcc cgcaggcttg ggtggaagaa ctggaaagtg gctatgttcc
     1981 accggaaaac tgggagcggg gtattaatgc gttttatact tcttattatg tgagtcagta
     2041 ttacagcgac tataaagcgt cgggtaacaa caagagtaca tatgtacgtt ttaacagcgg
     2101 gttaaattta ctggagtggc aactgcattc tgatgccagt ttcagtaaaa caaataacaa
     2161 tccaggggtg tggaaaagca ataccctgta tctggaacgt ggatttgccc aatttctcgg
     2221 cacgcttcgc gtgggtgata tgtacacatc aagcgatatt tttgattctg ttcgcttcag
     2281 cggtgtgcgg ttgtttcgtg atatgcagat gttgcctaac tcgaaacaaa attttacgcc
     2341 acgggtgcag gggattgctc agagtaacgc gctggtaact attgaacaga atggttttgt
     2401 ggtttatcag aaagaggttc ctcctggccc gtttgcgatt acagatttgc agttggccgg
     2461 tggtggagca gatcttgatg ttagcgtgaa agaggcggac ggctcggtaa ccacctatct
     2521 ggtgccttat gcagcggtgc caaatatgct gcaacccggc gtgtcgaaat atgattttgc
     2581 ggcgggggcg agcaagcaaa gtgattttgt ccaggcaggt tatcagtatg gttttaataa
     2641 cttattgacg ctgtatggcg gcacgatggt tgctaataat tactatgcgt ttaccctcgg
     2701 aacgggttgg aacacgcgca ttggcgcaat ttccgtcgat gccacgaagt cgcatagtaa
     2761 acaagacaac ggcgatgtgt ttgacgggca aagttatcaa attgcctaca acaaatttgt
     2821 gagccaaacg tcgacgcgtt ttggtctggc ggcctggcgt tattcgtcgc gtgattaccg
     2881 gacatttaac gatcacgttt gggcaaacaa taaagataat tatcgccgtg atgaaaacga
     2941 tatctatgac attgccgatt attaccagaa cgattttggc cgtaaaaata gcttttctgc
     3001 caatatgagc cagtcattgc cagaaggctg gggttctgtg tccttaagta cgttatggcg
     3061 agattactgg gggcgtagcg gcagcagtaa ggattatcag ttgagttatt ccaataactg
     3121 gcggcggata agctataccc tcgcggcaag ccaggcgtat ggcgagaatc atcatgaaga
     3181 gaaacgtttt aatattttta tatcaattcc ctgtgattgg ggtgatgacg ttacgacgcc
     3241 tcgtcggcaa atatatatgt ctaactcaac gacgtttgat gatcagggtt ttgcctcaaa
     3301 taatacggga ttatcaggaa cagtagggag tcgggatcag ttcaattatg gtgtcaacct
     3361 gagtcatcaa catcagggaa atgaaacgac agctggggcg aatttgacct ggaacgcgcc
     3421 ggttgcgaca gtgaatggca gttatagtca gtcgagtact tatcgacaga ctggagccag
     3481 tgtttcaggg ggcattgtcg cctggtcggg tggcgttaat ctggcgaacc gtctttccga
     3541 aacgtttgct gtgatgaatg cgccaggaat taaagatgct tatgtcaatg ggcaaaaata
     3601 tcgcacaaca aaccgtaatg gagtggtggt atacgacgga atgacacctt atcgggaaaa
     3661 tcacctgatg ctggatgtgt cgcaaagcga tagcgaagca gaattacgtg gcaaccggaa
     3721 aattgccgcc ccttatcgcg gcgcggttgt actggttaat tttgataccg atcagcgcaa
     3781 gccctggttt ataaaagcgt taagagcgga tggtcaacca ttaacgtttg gttatgaagt
     3841 caatgatatc catggtcata atattggcgt tgtcggccag ggaagccagt tatttattcg
     3901 caccaatgaa ataccgccat cggttaatgt agcaattgat aagcaacaag gactttcatg
     3961 cacaatcacc ttcggtaaag agattgatga aagtagaaat tatatttgcc agtaatagtc
     4021 aggtggttct atggaaattc gcataatgct atttatatta atgatgatgg ttatgcctgt
     4081 gagctatgcg gcatgttata gtgagttatc tgttcagcac aatttggttg ttcaggggaa
     4141 ttttgcactt actcaaacac aaatagcgac atatgagcat aattttaatg attcgtcatg
     4201 cgtgagtaca aatactatca cccctatgag cccatcggat attattgttg gactttataa
     4261 cgataccatt aaattaaatt tacattttga atggaccaat aaaaacaaca tcacgttgtc
     4321 aaataatcag agcagtttca ccagtggtta ttcggtaacg gtgacacctg cggccagtaa
     4381 tgcaaaagtg aatatttctg cggggggcgg tggttcagtg atgattaatg gtgttgcgac
     4441 attatccagt gcttcatcat cgacacgcgg gagtgccgca gtacaatttc tactgtgttt
     4501 attaggtggc aagtcatggg gtgcatgtgt aaatagctac agaaatgcat tggcacaaaa
     4561 tgcaggtgtc tattccttca atctgacatt gtcatacaac ccgataacca caacctgcaa
     4621 acaggacgat ttattaatta ctttagacag tattcccgtt ttacaattac cagccacagg
     4681 taacaaagca acaataaata gtaaaaaaag ggatattatt ctgcgttgta aaaatttatt
     4741 aggtcaacaa aatcaaacat cacggaaaat gcaggtgtat ttatcaagtt ctgacttgtt
     4801 aaccaacagc aacacaatac tgaaaggtgc ggaagataat gcgtaggatt tattcttgaa
     4861 agtaatggtt cgccagtcac acttttaaat atcactaaca gcagtaaagg atacacaaat
     4921 ttaaaagaaa ttgcggcaaa gtcaaaactt acagatacaa cggtttcaat tccgataaca
     4981 gccagttact acgtctacga tacaaacaaa gttaaatctg gcgcactgga ggcaaccgca
     5041 ttaatcaacg tgaaatacga ctaataacaa gcaaaacgag caagtggcta ataataaaaa
     5101 ataacaaggt gtctgcattt ccccctcatg atgagggggc tttttttagc gatgataaaa
     5161 aatctcaccg tcgtaggctt taatgatttt accgtcggtg tcgctgatca acacgtacgc
     5221 gccacccata taggtccagt gcgtcccggc atcaggcgcg ggcaggttac gtaactgcca
     5281 ctgcttaatg ttgtactcat cggtgcggta catctctggg acggtatcac ctggtttgaa
     5341 atgcttaaag tcagcaatga aactacttaa ctcatactta gaaattcccg aggtattggt
     5401 cgaacccgcg gtggctggtg cggcccaggc ggcactggta accagcaaaa gcacacccag
     5461 caacatctta tttttaatgg tcataaactc tccactttgg ggatctgtaa tatcagtcgc
     5521 atatctttcc tgtcatacaa tataactgac aagcgatatt ttgttagtga tcccatgatt
     5581 tcgtaaaaag tgtaaatgaa aggatgccct aaaagcatcc ttagcgagca gagagtcagc
     5641 ggaaatagct gatttaagaa tcatcctggg ctgaagataa catgcaagcc taaaggattg
     5701 agttatcgca ttatgcccat gaagccatgt acgcccatat acacaccgac taagccaatc
     5761 aacagactgg aaaaataggg ggcgcgttta gcgagagtgt taaatccgct ccagcgtttt
     5821 gcgacctgct gaacgctgat tgctgcgcca acgcctacgg tgacaagcgt taacgccagg
     5881 ccaatgctga aactgacgac cagtgttgcg cccagtgtca gggctttcaa ctgaatgcat
     5941 atcaacagca cggtaattgc tgccgggcag gggataaagc caccggttaa gccaaataac
     6001 aaaatttgcc agttgatgac ctctctacca tcaaagcgtc gtttaatgtc attggcatgg
     6061 gctcgtgcat gggcatcctg atactcgcca tgttcgtgat ggtgatgatg tccttggtcg
     6121 tggtgatgtt cgtgatcgtg atgatggtga tgatgctcat aatcatgccc gtgcatattc
     6181 tccagccagt tgcgttcgcc gcgctaggta cgccagaaca tccagaacgc ggtgccaata
     6241 atgatcactg cggaaatcag ctggagccac ggttctgctg attgagcagt aaagcgctta
     6301 ctgatcacca tcccgccaaa ggcaatcaac cagaccaccg cggtatgcga aatggttgct
     6361 gccagtccga gcatcaccgc ctgtttaatg gtgcctttga tggcgatgat aaacgccgcc
     6421 atcatcgttt ttgagtgccc tggttccagg ccatgaagcg caccaagtaa gatggcgctg
     6481 gggatgaaga accaggcgtt tccttgctga agaagagttg taaattcggt catgataata
     6541 attcttagta ttaattcggc aatctgattc tactcccccc cagtacctga tactaccccc
     6601 ccagtagatt aatagtgcta tgatttttca tattcttgtt aaccaggtgt tgccatgtct
     6661 catacaatcc gtgataaaca gaaactgaaa gcgcgtgcca gtaagattca gggccaggtc
     6721 gtggcgctca agaaaatgct cgacgagccg cacgaatgcg ctgcagtttt acaacagatt
     6781 gctgctatcc gtggagcggt aaacggtctg atgcgggaag tgattaaagg tcatctgacg
     6841 gaacacatcg ttcaccaggg ggatgagcta aaacgtgaag aagatctgga tgtcgttctg
     6901 aaggtgctgg attcatatat caaataattt attaacgcga ttgtaaaact gccgtttttc
     6961 ctcgtttaca acgggggcgc tggacattac catcctcctc tgcgatttat catcgcaacc
     7021 aaacgactcg gggtgccctt ctgcgtgaag gctgagaaat acccgtatca cctgatctgg
     7081 ataatgccag cgtagggaag tcacggacca ccaggtcatt gcttcttcac gttatggcag
     7141 gagcaaacta tgcaagtcga cctgctgagt tcagcgcaat ctgcgcacgc gttacacctt
     7201 tttcaccaac attcccctct tgtgcactgc atgaccaatg atgtggtgca aacctttacc
     7261 gccaatacct tgctggcgct cggtgcatcg ccagcgatgg ttatcgaaac cgaagaggcc
     7321 agtcagtttg cggctatcgc cagtgccttg ttgattaacg ttggcacact gacgcagcca
     7381 cgcgcacagg cgatgcgtgc tgccgttgag caagcaaaaa gctctcaaac acgctggacg
     7441 cttgatccag tagcggttgg tgcgctcgat tatcgccgcc atttttgtca tgaactttta
     7501 tcttttaaac cggcagcgat acgtggtaat gcttcggaaa tcatggcatt agctggcatt
     7561 gctaatggcg gacggggagt ggataccact gacgccgcag ctaacgcgat acccgctgca
     7621 caaacactgg cacgggaaac tggcgcaatc gtcgtggtca ctggcgaggt ggattatgtt
     7681 accgatggac atcgtatcat tggtattcac ggtggcgatc cgttaatgac caaagtggta
     7741 ggaactggct gtgcattatc ggcggttgtc gctgcctgct gtgcgttacc aggcgatacg
     7801 ctggaaaatg tcgcatctgc ctgtcactgg atgaaacaag ccggagaacg cgcagtcgcc
     7861 agaagcgagg ggccaggcag ttttgttcca catttccttg atgcgctctg gcaattgacg
     7921 caggaggtgc aggcatgaaa cgaattaacg ctctgacgat tgccggtact gatccgagtg
     7981 gtggtgcggg gattcaggcc gatcttaaaa ccttctcggc acttggcgct tatggttgct
     8041 cagttattac tgcactggtg gcgcaaaata cccgtggcgt acagtcggtg tatcgcattg
     8101 agcctgattt tgtcgccgcc cagctcgatt cggtgttcag cgatgtgcga atcgatacca
     8161 ctaaaatcgg tatgttggcg gaaaccgata ttgttgaagc ggtggcagaa cggttgcaac
     8221 gttatcagat ccaaaacgtg gtactcgaca ccgttatgct ggcaaaaagc ggcgacccgc
     8281 tgctttcacc ttcggcggtt gctacgctgc gcagtcgatt attgccacag gtttcattaa
     8341 taacgccaaa cttgcccgaa gctgccgcct tgctcgacgc gccacacgcg cgcaccgaac
     8401 aggaaatgct ggaacaaggg cgatcgctgt tggcgatggg ctgtggcgca gtgctaatga
     8461 aaggtggtca tctggatgat gagcaaagcc cggactggct gtttacccgc gagggtgaac
     8521 aacggtttac cgcaccgcgc attatgacca aaaacaccca cggcactggt tgtacactct
     8581 ctgcggcgtt ggctgcacta cgcccgcgcc atacaaactg ggctgacacc gtacaggagg
     8641 caaaaatctg gctttcatcg gcgttagccc aggccgacac gctggaagtt ggtcacggta
     8701 ttggtccggt tcaccacttc cacgcctggt ggtgagcacc gtactatagc tgctttggtt
     8761 attgtcagcc tttggcactg acattcaggg atgaaaccaa tgcagctaag aattaccagt
     8821 cgaaaaaaac tcaccgctct attatgcgct cttgggctaa tttccatcgt tgcaatttat
     8881 ccgcgtcaga cggtgaattt tttctattcg acagcagttc agattacaga ctacatccac
     8941 ttctacggtt atcgcccggt taaatctttc gctattcgca ttcctgccag ttacaccatt
     9001 cacggattag atgtttcgcg ctggcaggag cgtatcgact ggcagcgggt ggcaaaaatg
     9061 cgcgacaacg atatccgctt acagtttgct tttattaagg ctacggaagg cgaaaagctg
     9121 gtggacccct atttttcgcg taactggcaa ttaagccgcg aaaatggcct gctgcgcggg
     9181 gcgtatcatt atttctcccc gtcggtatcc gcttcagttc aggcgcgatt atttctgcaa
     9241 acggtggatt tctcacaagg cgatttccct gccgtgctgg acgtagaaga acggggaaaa
     9301 ttatcggcaa aagaattacg caagcgggta agtcagtggc taaaaatggt cgaaaaacgc
     9361 acgggaaaaa agccgattat ttactcagga gccgtttttt atcacaccaa tctggcgggc
     9421 tatttcaatg aatatccgtg gtgggtggct cactattatc aacgtcgtcc ggacaatgac
     9481 ggcatggcct ggcgcttctg gcagcattcc gaccgtggac aggtagatgg catcaatggt
     9541 ccggtggatt ttaatgtgtt taatggcacg gggatgagtt gcaggcattc gttgatggga
     9601 ttaaagaaac gccttaaata agaagcagtg atatactccc accagactcc ctggcaggaa
     9661 aaagacaaat ggaacaagcg catacccagc tgattgccca actgaacgag cgtattttag
     9721 cggcggataa cacgccgctt tatattaagt ttgccgaaac ggtaaaaaat gccgtgcgca
     9781 gcggggtgct ggagcatggc aatattttgc ccggtgagcg tgatttaagt cagttaaccg
     9841 gcgtttcgcg cattacggtg cgcaaggcga tgcaggcgct ggaagaagag ggcgtggtga
     9901 cgcgttcgcg cggttacggc acgcagatca acaacatctt cgaatactcg ttgaaagaag
     9961 cgagaggttt ttctcagcag gtggtattgc gcgggaaaaa gcccgatacg ctatgggtta
    10021 ataagcgtgt cgtgaaatgt cccgaagaag ttgcgcagca gctggcggcc gaagcgggaa
    10081 gtgatgtctt tttgcttaag cgtattcgct atgtcgatga agaagcggta tcgattgagg
    10141 aatcgtgggt tccggcgcat ttaattcatg atgttgatgc catcggaatt tctctttatg
    10201 actatttccg cagccagcac atttacccac aacgtacgcg ttcccgcgtt agcgcccgga
    10261 tgccggatgc cgagtttcag tcacatattc agttagatag caaaataccg gtgctggtga
    10321 tcaagcaagt ggcgcttgac caacagcaac ggcctattga gtacagcatt agccactgtc
    10381 gcagcgatct atacgttttt gtgtgcgagg agtagttcct cgcgcgttgg cgcacaatca
    10441 ccgccccgat gcccgacaac ccacgacgcc actgcattgc ccagtaatac ggcatccgcc
    10501 agtggcagac cagaggccag cccggcaagt acgccacctg catgactgtc gcccgcccca
    10561 atggtgtcta caacttgcgt tggaaatgcc ggaatgcagc cagaagcgtc gttgctgaaa
    10621 taccatgcgc cttctttatc gagacgaacg atcaacggcg cggcaaattt ctcctgccat
    10681 tgcttgccaa gtgttgttat ctctgcggat aaagcaaaac gttcggcggc aatctcagcc
    10741 tcttgacgat tgagtgacac taaaggtcga caggccatga tccgcgccag taatgcgtcc
    10801 gggatatcgc caatacgtgg gcaaaaatcg ataaacggcg tcacgtcttg cagcttttcc
    10861 agccattcca ctaacaattc gccgcagggc gaggccagtt gataaccgga aaaatagagc
    10921 aggttgccag gcgcaacggt taatcgtgcc aaccattggc gattccattg attttccaca
    10981 ccgctgaatg acataaaagt gcgttcgcca tccggctcaa ccagtgccag acaccagccg
    11041 ttatcgcttt cggcgttatc gatcaggctg attaacccct ctttcgccat ccggttgcga
    11101 ataatctccg cccacacgcc ctgaccgagc ggcaaggcat tacccgcttc gatgccgagg
    11161 cgctttaacg ccacggcaat attcagtgcg cagccgccaa cgttaacgct ctgctgtttc
    11221 agttcgatat cgcacccacg ccaggggagg gcataagcgt cggcgatcac gtcaatgacc
    11281 gccgcgccga cgaccatcac cggctgacgc gtggttaatt caggcagcag tgtgtgtaat
    11341 cgagcgccgc tcataccgcc tcccgttgtt gacgatattt cgccagcgct gtggcatagc
    11401 ggttgaaatc aagctgattt accgcatcca gctccgcctt taacgcagga tcgacagcgt
    11461 taatgccatg caacgcgccg caaatcgccg tcgccatagc acctatggtg tctgtgtcgc
    11521 cgccaagatt ggcgcacagg acggcgcagc ggtttgggtc ggtttgtgcc agttcaacta
    11581 gcgcaatggc gcacggaacg gactcaatag tgctggtgcc tgcgccaacg acctggtaaa
    11641 gctgttcgcg ggcggattcg gtgccgtcgg cattacgcac aattttcagc gcaatctcca
    11701 gacgtgccgc cagtgaggcg ctgaacgtgg tggtgcgttt ttgctgtgca tgtcgcgcaa
    11761 ttgaaggcaa tgaatcaaca atcgctgacc agcgttctcc gtcaatggca cgagaaatcg
    11821 cccatgcgat gactaccgcg cctgcaaccg ccagatcgga tttatgggtc gggctggacg
    11881 ccagcgccac atcatcaata aaggaatcaa catcacgcgc tggaagcaaa caacctaatg
    11941 gcgagacgcg catcgctgcg ccgttggtta cgccgttgtt ttccagttca gcaacgggtt
    12001 taccgtcgcg aatggcgtta agcgcaatct tcgaggtcgg acctagtacg tttttgttaa
    12061 aggcgtcgaa acgcaacgcc cagtcgagaa tattacgccc aatcagattc ggatcgatct
    12121 tgccttcaca ttccagtaac gcatccgcca gacacaggcc atcgaggtat cgtcggtgaa
    12181 ttcggcgcgg ttaaaataac aggccgcgtt attctccttt ggtccaggca gaaaacggtc
    12241 aatccagcca aagtgtgctt taacgcggct gcgtggccaa agctcggagg gcatccccat
    12301 cgcatcccct aacgcctgcc cataaagagc accgagaata cgttctgttt tcatttaact
    12361 tccccttgaa tcggaattta cgggagcata gtaatgacag atattgcgca gttgcttggc
    12421 aaagacgccg acaacctttt acagcaccgt tgtatgacta ttccttctga ccagctttat
    12481 ctccccggac atgactacgt agaccgcgtg atgattgaca ataatcgccc gccagcggtg
    12541 ttacgtaata tgcagacgtt gtacaacacc gggcgtctgg ctggcacagg atatctttct
    12601 attctgccgg ttgaccaggg cgttgagcac tctgccggag cttcatttgc tgctaacccg
    12661 ctctactttg acccgaaaaa cattgttgaa ctggcgatcg aagcgggctg taactgtgtg
    12721 gcatcaactt acggcgtgtt ggtgtcggta tcgcggcgct atgcgcatcg cattccattt
    12781 ctcgtcaaac ttaatcacaa cgagacgcta agttacccga atacctacga tcaaacgctg
    12841 tatgccagcg tggagcaggc cttcaacatg ggcgcggtgg cggttggtgc gactatctat
    12901 tttggctcgg aagagtcacg tcgccagatt gaagaaattt ctgcggcctt tgaacgcgcg
    12961 cacgagctgg gcatggtgac agtgctgtgg gcctatttgc gaaactccgc ctttaagaaa
    13021 gatggcgttg attaccatgt ttccgccgac ctgactggtc aggcgaacca tctggcggca
    13081 accataggtg cagatatcgt caaacaaaaa atggcggaaa ataacggcgg ctataaagcg
    13141 attaattacg gttataccga cgatcgcgtg tacagcaagt taaccagcga aaacccgatt
    13201 gatctggtgc gttatcagtt agctaactgc tatatgggcc gggctgggtt gataaactcc
    13261 ggcggtgctg caggcggtga aactgacctc agcgatgcag tgcgtactgc ggttatcaac
    13321 aaacgcgcag gcggaatggg gctgattctt ggacgtaagg cgttcaagaa atcgatggct
    13381 gacggcgtga aactgattaa tgccgtgcag gacgtttatc tcgatagcaa aattactatt
    13441 gcctgatacg cctcatacga acagcaccac cccgtaggac ggataagacg tttacactgc
    13501 atccggcaac ctatgcctga tgcgacgcgg aagcgtctta tcatgcctac atagcactgc
    13561 cacgtatgtt tacaccgcat ccggcataaa aacacgcgca ctttgctacg gcttccctat
    13621 cgggaggccg tttttttgcc tttcactcct cgaacaattt tcatattgtt gtttttgcga
    13681 tcattatctc gacatttaaa agcaaataat ttcattatat tttgaaatcg aaagcaaacg
    13741 acaagatatg aaaatgtacg tggtatcgac aaagcagatg ctgaacaacg cacagcgcgg
    13801 cggttatgcg gttccggcat tcaatattca caatctcgaa acgatgcaag tggtggtgga
    13861 aaccgctgcc aacctgcatg cgccggtcat catcgccgga acgcctggca catttactca
    13921 tgctggtaca gaaaatctgt tggcgctggt cagcgcgatg gcgaagcaat atcaccatcc
    13981 actggcaatt catctcgacc atcacacgaa atttgacgat atcgctcaga aggttcgttc
    14041 tggcgtgcgc tcagtcatga ttgacgcctc gcatttgcct tttgcgcaaa atatttcacg
    14101 ggtcaaagag gtggtggatt tttgccatcg ctttgatgtc agcgtcgagg cggagctggg
    14161 gcaacttggc ggccaggaag atgatgtgca agtcaatgaa gtcgatgcgt tgtacaccaa
    14221 ccccgcacag gcgcgtgaat ttgccgaggc aaccgggatt gattccctgg cggtcgccat
    14281 cggcacggct cacggtatgt atgccagcgc accggtgctt gatttttctc gactggagaa
    14341 cattcgccag tgggtgaact taccgctggt gctgcatggc gcgtcaggat tatcgactaa
    14401 ggatattcag caaaccatca aactggggat atgcaaaatc aacgttgcaa cggagctgaa
    14461 aaatgccttc ttgcagtcgt taaaaaatta cctgaccgag caccccgaag cgaccgatcc
    14521 ccgggattat ttgcagtcgg ctaaatccgc aatgcgcgat gtggtgagca aagtgattgc
    14581 cgattgtggc tgcgagggca gggcataacg cacctgccat ttaacaagga aaaaacatga
    14641 aaacgttaat tgcccggcat aaagctggtg aacatatcgg catatgttca gtctgttctg
    14701 cccatccgtt ggttatcgaa gcggcgctgg catttgatcg caacagcacg cgcaaagtgc
    14761 tgattgaagc aacgtcaaac caggtcaatc aatttggcgg ttataccgga atgacaccgg
    14821 cagactttcg cgaatttgtt tttacgattg ccgataaagt tgggtttgca cgcgaacgca
    14881 ttattctcgg cggcgatcat ctgggaccaa actgctggca gcaagaaaat gcggatgcgg
    14941 cgatggaaaa atccgtcgag ctggtaaaag catatgttcg tgctggcttc agtaaaattc
    15001 atcttgatgc gtcaatgtcc tgcgcggggg ataccatacc gttagcacca gaaacggttg
    15061 cggaacgagc tgctgtgctt tgctttgctg cggaaagtgt ggcgacagat tgccagcgtg
    15121 agcaactgag ctatgtcatt ggcactgaag ttccggttcc gggcggtgag gccagcgcca
    15181 ttcagtcagt acacatcacc catgttgaag atgccgccaa tactttacgt acgcatcaaa
    15241 aggcctttat tgcccgtggg ctgacagagg cgttaacacg tgtgattgcc atcgtggtgc
    15301 agccgggtgt ggaatttgat cacagcaata ttatccatta tcagccgcag gaagcgcagg
    15361 cactggcgca atggatagaa aacacccgaa tggtttatga agcacattct accgattacc
    15421 agacccggac ggcttattgg gaattagtcc gcgatcactt tgcaatattg aaagtcggtc
    15481 ccgcattaac ctttgcttta cgcgaggcga tatttgcact ggcacaaatt gagcaggaac
    15541 ttatcgcccc cgaaaatcgc agcggttgcc tggcggtaat tgaagaagtg atgctcgacg
    15601 aaccgcaata ctggaaaaaa tattatcgca cgggttttaa cgattcatta ctggatattc
    15661 gttacagcct gtcggatcgt attcgttatt actggccgca tagtcggatt aaaaatagcg
    15721 ttgaaacgat gatggtgaat ctgcaaggcg tggacatccc actgggcatg attagtcagt
    15781 atcttcccaa acaatttgaa cgcattcagt ccggggaatt atcagcaatg ccgcatcagc
    15841 ttattatgaa taaaatttat gatgttttgc gcgcctatcg ctacggctgt gcggaataag
    15901 gacggtatat gactaacctg tttgttcgta gcggaatttc ttttgtcgat cgtagcgaag
    15961 ttttaaccta tatcggtaat gagatgctcg cgaaaggtgt ggttcatgat acctggccac
    16021 aggcattaat tgccagagaa gcagaattcc ctaccgggat aatgcttgag cagcacgcca
    16081 ttgcaatacc gcattgtgag gcgattcatg ctaagtcgtc agccatttat ctgttaaggc
    16141 caacaaataa agttcatttt cagcaagcgg atgatgataa cgacgtggcg gtatcgttgg
    16201 ttattgcgtt gattgtggaa aatccgcagc agcaattgaa acttttacgc tgtttatttg
    16261 gcaagttaca acagcccgat atcgtcgaga cactaattac tcttcctgaa actcagttaa
    16321 aggaatactt cacaaagtat gttttagatt cagacgaata aatccctctg taacaataaa
    16381 aaggactatt tatgaaacgc aagattattg tcgcttgcgg aggcgcggtt gcgacctcta
    16441 cgatggcggc ggaagaaatt aaagagttgt gtcagagtca taatattcct gttgaattaa
    16501 tccagtgtcg ggttaatgaa atagaaacct atatggatgg tgtgcatttg atatgtacca
    16561 ctgccagggt ggatcgtagt tttggcgata ttccgttagt tcacggcatg ccttttgttt
    16621 ctggtgtcgg tatcgaagca ttacaaaata aaattctgac tatcttacag ggatgaccta
    16681 tgttttcaga agtcatgcgt tatattctcg acctcggccc tacagtgatg ctgccggttg
    16741 tcatcattat tttttctaaa atattaggca tgaaggcagg cgattgcttt aaagcgggtc
    16801 tgcatatcgg gattggcttt gttggcattg gccttgtgat tggcttaatg ctggattcca
    16861 ttggtccggc ggcgaaagcg atggcggaaa atttcgacct gaatctgcat gtggtcgatg
    16921 tcggctggcc gggctcttca ccaatgacct gggcgtcgca aattgcgctg gtggcgattc
    16981 cgattgcgat attggttaac gtggcgatgt tactgacccg tatgacgcgg gtggtaaatg
    17041 ttgatatctg gaatatctgg catatgacct tcaccggcgc gttgctgcac ctggcaaccg
    17101 gttcatggat gatagggatg gcgggtgtgg taattcacgc ggcgtttgtt tataagctcg
    17161 gcgactggtt tgcccgcgat acccgaaatt tctttgagct ggaaggtatt gctattccgc
    17221 acggtacgtc ggcgtatatg gggccgattg cggtgctggt cgatgctatc atcgagaaaa
    17281 tcccaggcgt taaccgaatt aaatttagcg ccgacgatat tcagcgcaaa tttggccctt
    17341 ttggcgaacc tgtcaccgtg ggttttgtga tggggctgat tatcggcatc ctcgcgggtt
    17401 acgatgtcaa aggtgtattg cagctggcgg taaaaacggc ggcagttatg ctgctgatgc
    17461 cacggatgat taaacccatc atggatggtt taacgcccat cgctaagcag gcccgtagtc
    17521 gtttacaggc gaagttcggc ggtcaggagt tcctgattgg cctggatccc gcattactgc
    17581 tggggcatac ggcggtggta tcggcaagcc tgatttttat tccgctcacc attttaattg
    17641 ctgtttgtgt tccgggtaat caggtactgc cgtttggcga tcttgccact atcggcttct
    17701 tcgtggcgat ggcggtcgcg gtgcatcgtg gaaatctgtt ccgcacctta atctcgggtg
    17761 tcatcattat gagcatcacc ctgtggatcg cgacgcaaac tattggtttg cacacccaac
    17821 cggcggctaa tgctggggcg ttaaaagccg ggggtatggt ggcttcaatg gatcagggcg
    17881 gttctcccat tacctggtta ctgattcagg ttttctcccc gcaaaatatt cccggtttca
    17941 ttattatcgg cgcaatttat ctgaccggta ttttcatgac ctggcgtaga gcgcgtggtt
    18001 ttactaaaca agagaaagcc gttctcgcag aataattttt acctgagggg gaggttatcc
    18061 cccttaacaa
//
LOCUS       DAJTUI010000078        17758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTUI010000078 DAJTUI010000000
VERSION     DAJTUI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.078"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            446..1129
                     /locus_tag="OO464_002894"
     CDS             446..1129
                     /locus_tag="OO464_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6227971.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1173..5835
                     /locus_tag="OO464_002895"
                     /pseudo
     CDS             1173..5835
                     /locus_tag="OO464_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6203..7858
                     /gene="lldP"
                     /locus_tag="OO464_002896"
     CDS             6203..7858
                     /gene="lldP"
                     /locus_tag="OO464_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6227972.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7858..8634
                     /gene="lldR"
                     /locus_tag="OO464_002897"
     CDS             7858..8634
                     /gene="lldR"
                     /locus_tag="OO464_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6227973.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8631..9821
                     /gene="lldD"
                     /locus_tag="OO464_002898"
     CDS             8631..9821
                     /gene="lldD"
                     /locus_tag="OO464_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6227974.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10017..10490
                     /gene="trmL"
                     /locus_tag="OO464_002899"
     CDS             10017..10490
                     /gene="trmL"
                     /locus_tag="OO464_002899"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6227975.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10543..11364)
                     /gene="cysE"
                     /locus_tag="OO464_002900"
     CDS             complement(10543..11364)
                     /gene="cysE"
                     /locus_tag="OO464_002900"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6227976.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11444..12463)
                     /gene="gpsA"
                     /locus_tag="OO464_002901"
     CDS             complement(11444..12463)
                     /gene="gpsA"
                     /locus_tag="OO464_002901"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6227977.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12463..12930)
                     /gene="secB"
                     /locus_tag="OO464_002902"
     CDS             complement(12463..12930)
                     /gene="secB"
                     /locus_tag="OO464_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6227978.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12993..13244)
                     /gene="grxC"
                     /locus_tag="OO464_002903"
     CDS             complement(12993..13244)
                     /gene="grxC"
                     /locus_tag="OO464_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6227979.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13386..13817)
                     /locus_tag="OO464_002904"
     CDS             complement(13386..13817)
                     /locus_tag="OO464_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6227980.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14062..15606
                     /gene="gpmM"
                     /locus_tag="OO464_002905"
                     /gene_synonym="pgmI"
     CDS             14062..15606
                     /gene="gpmM"
                     /locus_tag="OO464_002905"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6227981.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15616..16899
                     /gene="envC"
                     /locus_tag="OO464_002906"
     CDS             15616..16899
                     /gene="envC"
                     /locus_tag="OO464_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6227982.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16903..>17758
                     /locus_tag="OO464_002907"
     CDS             16903..>17758
                     /locus_tag="OO464_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6227983.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVL"
BASE COUNT         4471 a         4585 c         4595 g         4107 t
ORIGIN      
        1 aattcactcc ctttacttcg atttaccgct ggttgatatt ctgaaaatgc atttttaccg
       61 aaatagccat ctataaacaa taaaaatata tagaagatat catttgttaa ttaacaagca
      121 tttacgtctc tttttttcgg atttaattgt gaaattttaa tcatcatatt cacaaaatca
      181 attatctgtg aaatttttcc cagacatggt ttctttcata tttgacattt attaataatc
      241 atcattagaa tccacgaaaa ataatatatt catacaaaca cagacatctt ttgaaacgaa
      301 tgatacattt ttatgatgaa tgcttaaaac aatatttaat cgacacacat cactgtcatt
      361 aatgtaaagc aacatagctc accaataata caatggacaa ctctaccggc attgagcgct
      421 ggtcggagga aataaggatt tttacatgaa cttgaagaag atttttttca gtgctgtcac
      481 tgtttcagtt ctctgcgcat taacgggatg tgactacatt gaagaaggca agccagaaag
      541 tagtttactg aaacaacaag aagaacataa taataaaatt gtgctacttg aaaaacaaca
      601 agcacagcta aagagtcaac tcgaaactat tcaaaaacaa caaactggca taataaacag
      661 tacaaagact ttaactcacg taattaaatc cgttaaagac caacaaaata cttttatttt
      721 taccgagttt aacccagcaa aaacgaagta tttcatcctg aacaacggtt cggtggcttt
      781 agcgggtcga gtgttatcca ttgacgccac agagaatggt agtgttattc atatctcact
      841 ggtcaactta ttaagtacac ctatctcaaa cattggtttt aatgcgacat ggggtggtga
      901 aaaacctgtc gatgccaaag agtttgcccg gtggcaacaa ttacttttca acacatcaat
      961 gaaatccaca ttgaaattat taccaggtca atggcaagac attaatttga ccctgaaggg
     1021 tgtatcgccc aataacctgg gatatctgaa gttagccatc aacatggaaa atattcagtt
     1081 cgacaatctt ccctctgctg aaaatcggca gaaaagaagc aaaaaataaa tgcagttagt
     1141 acgcttataa caaggaatag cgagcatcat taatgaacaa aatatttaaa gttatctgga
     1201 accctgcgac agggaattat actgttacca gcgaaacggc aaaaagccgt ggcaagaaat
     1261 ctgggcgcag taagctgtta atttctgcgc tggttgcggg tggaatgttg tcgtcgtttg
     1321 gggcattggc gaatgccggg aatgacaacg gtcagggtgt tgattacggt agtggatcag
     1381 ctggcgacgg ctgggttgct ataggcaaag gggcgaaagc aaatactttt atgaacacca
     1441 gtggttccag tactgctgtg ggttatgacg ctatagctga aggccaatat agctctgcca
     1501 tcgggtcaaa aacccatgcg attggtggtg catcaatggc ctttggggtt agtgcaatat
     1561 cagaaggcga tagaagtata gcactgggtg cctcttcgta ttcattgggc caatactcaa
     1621 tggccctcgg ccgttattca aaagcattgg gtaaattgtc tattgctatg ggggactctt
     1681 ccaaagcgga aggagcaaac gccattgccc tggggcgtag cagtgtagct agcggtactg
     1741 acagcctcgc atttggcaga caatcacttg ccagcgcagc gaacgctatt gcgataggtg
     1801 ctgagaccga agccgctgaa aatgcaactg ctattggcaa taatgcgaag gcaaaaggga
     1861 ctaatagcat ggcaatgggg ttcggaagcc ttgccgataa agtcaatact atcgcattag
     1921 gaaatggcag ccaggctctg gcagataatg caatcgccat aggccagggc aacaaagctg
     1981 atggcgtgga tgccatcgct ctgggtaatg gtagccagtc gagaggctta aacaccattg
     2041 ccttaggcac agccagtaat gcaactggtg ataagagtct tgcgcttggt agtaatagca
     2101 gtgccaacgg tattaactct gtcgcgctgg gcgcagattc cattgcggat ttagacaata
     2161 ccgtctctgt cggcaatagt tcattaaaac gcaagatcgt taatgtgaaa aatggcgcga
     2221 tcaagtctga cagttacgat gccattaatg gttcacagct ttatgccatt agcgactcgg
     2281 tagcaaaaaa ggcttggagg aggggctgca gtagatgttg atgacggtac tgttacagca
     2341 ccaacctaca atttaaaaaa tggtagcaaa aataacgtag gggctgcgct cgctgtactt
     2401 gatgaaaaca ccctgcaatg ggaccaaacc aaaggcaaat acagcgctgc tcatggtact
     2461 agtagcccaa ctgccagcgt aatcaccgat gttgcggatg gcacgatttc agcctccagt
     2521 aaggatgcgg ttaacggttc ccaactgaaa gctaccaatg acgatgtcga agccaacacc
     2581 accaatatca ccaacctgac ggattccgtt ggtgaccttc aggctgatgc cctgctctgg
     2641 aacgaaacta aaaaggcatt cagtgcagct cacggccagg ataccaccag caaaatcacc
     2701 aacgttaaag atgccgacct gacggctgac agcactgatg ctgttaacgg ctctcagctg
     2761 aaaaccacca acgatgctgt ggcgacgaat accaccaata tcgccaataa cacttccaat
     2821 attgccacta acaccaccaa catctctaac ctgactgaga cggtgactaa tcttggtgag
     2881 gatgcgctga aatgggataa ggacaatggt gtattcacgg cagctcatgg caccgagacc
     2941 accagcaaaa taaccaacgt taaagatggc gacctgacga ctggcagcac cgatgccgtt
     3001 aacggctctc agctgaaaac caccaacgat gccgtggcga cgaataccac caatatcgcc
     3061 actaacacca ccaacatctc taatctgact gagacggtga ctaatcttgg tgaggatgcg
     3121 ctgaaatggg ataaggacaa tggtgtcttc actgcagctc atggcaacaa taccgccagc
     3181 aaaatcacca atatcctgga cggcacagtc actgcaacca gttccgatgc cattaacggt
     3241 agccagcttt atgacttaag cagcaatatc gccacctact tcggcggcaa tgcttctgtg
     3301 aatactgacg gtgtgtttac cggtccaacc tacaaaatcg gtgaaacaaa ttattataac
     3361 gtcggcgatg cactggctgc gattaactcc tcatttagca cgtctctcgg cgatgctctg
     3421 ctttgggatg ccaccgcagg taaattcagt gccaaacacg gtactaatgg tgacgcaagc
     3481 gtgatcactg atgtcgcaga tggtgaaatt tcagactcca gttctgacgc agtaaacggc
     3541 tcacaactcc acggcgtgag cagttatgtt gttgatgcgc tggggggtgg tgccgaagtc
     3601 aatgcagacg gcaccatcac tgcgccgacg tacaccattg ctaatgctga ttacgataat
     3661 gtcggtgatg ccctgaatgc tatcgatacc actcctgacg acgctctgct ctgggatgcg
     3721 gacgccggtg aaaatggtgc atttagcgcc gctcacggaa aagataaaac tgccagtgta
     3781 atcactaacg tcgctaacgg tgcaatctct gctgccagca gcgacgcgat taacggctca
     3841 caactctata ccaccaataa gtacatcgct gatgcgctgg gtggtgacgc agaagtcaac
     3901 gctgacggca ccatcaccgc accgacttac accattgcga acgccgagta caacaacgtc
     3961 ggtgacgccc tggatgcgct tgatgataac gccctgctgt gggataagac tgccaatggc
     4021 ggtgctggag cctacaatgc cagccatgac ggtaaagcca gcatcatcac taatgtcgct
     4081 aatggcagta ttagtgagga cagtaccgat gcagtgaacg gttctcagtt gaatgcgacg
     4141 aatatgatga ttgagcagaa cacccaaatt atcaatcagc tcgctggtaa caccgacgca
     4201 acctatatcg aagaaaatgg cgctggtatc aactacgttc gtactaacga caacgattta
     4261 gcctttaacg atgcaagcgc ctctggtgtc ggcgctacag ctgtaggtta taacgctgtc
     4321 gcgtctggtg ccagcagcgt agccattggt cagaacagca gcagcaccgt tgataccggt
     4381 attgcgctgg gtagcagctc cgtttccagc cgtgtgattg ccaaaggttc tcgtgacact
     4441 agcgtaacgg aaaatggcgt ggtgattggt tatgacacca ctgacggcga actgctaggt
     4501 gcattgtcaa ttggtgatga cggtaaatac cgccaaatca tcaacgtagc tgatggttca
     4561 gaagcccatg acgccgttac ggttcgccag ttgcagaacg ctattggagc ggtcgccact
     4621 acgccaacca agtactttca cgccaactca acggcagaag actcactggc cgttggtgaa
     4681 gactcgctgg caatgggtgc gaaaactgtc gttaatggta atgcaggtat tggtatcggt
     4741 ttgaacactc tggttctggc tgatgcgatc aacggcattg ctatcggcag taacgcacgc
     4801 gcaaaccatg caaacagtat cgcaatgggt aacggttctc agaccacccg tggtgcacag
     4861 actggctaca ccgcctacaa catggacgca ccgcagaact ctgtaggtga gttttctgtc
     4921 ggtagtgaag acggtcaacg tcagatcaca aacgtcgcag ctggttcagc ggataccgat
     4981 gcggttaacg tgggtcagtt gaaagtcact gatgagcgcg tagcgcaaaa tacccagagc
     5041 attactaacc tgaacaatca ggtcactaat ctggatactc gcgttactaa tatcgaaaac
     5101 ggtattggcg acattgtcac caccggtagc accaagtact tcaagaccaa caccgatggc
     5161 gtagatgcca acgcccaggg taaagatagc gttgctattg gttctggttc cattgctgcc
     5221 gctgacaaca gcgtcgcact gggtaccggt tccgttgcag aggaagaaaa tacaatctct
     5281 gtaggttctt ctactaacca acgccgtatc accaacgtag ctgccagtgt taatgccacc
     5341 gatgcggtta acgtgtcgca gctgaaatct tctgaagcag gcggagtgcg ctacgacacc
     5401 aaagctgatg gttctatcga ctatagcaat atcaccctcg gtggtggcaa cggcagtacg
     5461 actcgtatca gcaacgtctc cgctggcgtc aacaacaacg acgcggtgaa ctacgcgcag
     5521 ttgaagcaaa gcgcgcagga aacgaagcaa tacaccgatc agcggatggt tgagatggat
     5581 aacaaactgt ctaaaactga aagcaagttg agcggtggta tcgcttctgc aatggcaatg
     5641 accggtctgc cgcaggctta tacaccgggt gccagcatgg cttctattgg tggcggtact
     5701 tacaacggtg aatcggcagt tgctttaggt gtatcgatgg tgagcgccaa tggtcgttgg
     5761 gtctacaaat tacaaggtag taccaatagc cagggtgaat actccgccgc actcggtgcc
     5821 ggtattcagt ggtaatgatc cattaagtta gtgtgactaa gggcaaacgc caaattgcct
     5881 gatgcgttac gcgcatcagg caagataagg cgctccgggt caacaatcta agggcaattc
     5941 tctgatgagg attgcccttt tctttaccag acatctcccc ccacaagaat tggccctacc
     6001 aattcttcgc ttatctgacc tctggttcac aattccccaa ttaaaactca catcaatatt
     6061 gccaatacat aacatttagt taaccattca ttgtcattat ccctacacaa cacaattggc
     6121 agtgccactt ttacacaacg tgtgacaagg agatgagcaa cagactcatt acacgatgtg
     6181 cgtggactct aggagacctg caatgaatct ctggcaacaa aactacgatc ccgccgggaa
     6241 tatctggctt tccagtctga tagcatcgct tcccatcctg tttttcttct ttgcgctgat
     6301 taagctcaaa ctgaaaggat acgtcgccgc ctcatggacg gtagcaatcg cccttgccgt
     6361 ggctttgctg ttctataaaa tgccggtcgc taacgcgctg gcctcggtgg tttatggctt
     6421 cttctatggt ttgtggccca tcgcgtggat cattattgca gcagtgttcg tctataagat
     6481 ctcggtgaaa accgggcagt ttgacatcat tcgctcgtct attctttcga taacccctga
     6541 ccagcgtctg caaatgctga tcgtcggttt ctgtttcggc gctttccttg aaggagccgc
     6601 aggctttggc gcaccggtag caattaccgc cgcattgctg gtcggcctgg gttttaaacc
     6661 gctgtacgcc gccggactgt gcctgattgt taacaccgcg ccagtggcat ttggcgcgat
     6721 gggcattcca atcctggttg ccggacaagt aacaggcatc gacagctttg agattggcca
     6781 gatggtgggg cgtcagctac cgtttatgac cattatcgtg ctgttctgga tcatggcgat
     6841 tatggacggc tggcgcggta tcaaagagac gtggcctgcg gtcgtggttg caggcggctc
     6901 gtttgccatc gctcagtacc ttagctctaa cttcattggg ccggagctgc cggacattat
     6961 ctcttcgctg gtatcactgc tctgcctgac gctgttcctc aaacgctggc aaccagttcg
     7021 cgtattccgc ttcggtgact tgggggcgtc acaggttgat atgacgctgg cccacaccgg
     7081 ttacaccgcg ggtcaggtgt tacgtgcctg gacaccgttc ctgttcctga ccgctaccgt
     7141 aacactgtgg agcatcccgc cgtttaaagc cctgttcgca tcgggtggtg cgctgtatga
     7201 gtgggtgatc aacattccgg tgccgtacct caataaactg gttgcccgta tgccaccagt
     7261 ggtcagcgag gctacagcct atgccgccgt gtttaagttt gactggttct ctgccaccgg
     7321 caccgccatt ctgtttgctg cactgctctc gattgtctgg ctgaagatga aaccgtctga
     7381 cgctatcagc accttcggca gcacgctgaa agaactggct ctacccatct actccatcgg
     7441 catggtgctg gcgttcgcct ttatctcgaa ctattccgga ctgtcatcaa cactggcact
     7501 ggcactggcg cacaccggtc atgcattcac cttcttctcg ccgttcctcg gctggctggg
     7561 ggtattcctg accgggtcgg atacctcatc taacgccctg ttcgccgcgc tgcaagccac
     7621 cgcagcacaa caaattggcg tctctgatct gttgctggtt gccgccaata ccaccggtgg
     7681 cgtcaccggt aagatgatct ccccgcaatc tatcgctatc gcctgtgcgg cggtaggcct
     7741 ggtgggcaaa gagtctgatt tgttccgctt tactgtcaaa cacagcctga tcttcacctg
     7801 tatggtgggc gtgatcacca cgcttcaggc ttatgtctta acgtggatga ttccttaatg
     7861 attgttttac ccagacgcct gtcagacgag gttgccgatc gtgtgcgggc gctgattgat
     7921 gaaaaaaacc tggaagcggg catgaagttg cccgctgagc gccaactggc gatgcaactc
     7981 ggcgtatcac gtaattcact acgcgaggcg ctggcaaaac tggtgagcga aggcgtgctg
     8041 ctcagtcgac gcggcggcgg gacgtttatt cgctggcgtc atgacacatg gtcggagcaa
     8101 aacatcgtcc agccgctaaa aacgctgatg gccgatgatc cggattacag tttcgatatt
     8161 ctggaagccc gctacgccat tgaagccagc accgcatggc atgcggcaat gcgcgccaca
     8221 cctggcgaca aagaaaagat tcagctttgc tttgaagcaa cgctaagtga agacccggat
     8281 atcgcctcac aagcggacgt tcgttttcat ctggcgattg ccgaagcctc acataacatc
     8341 gtgctgctgc aaaccatgcg cggtttcttc gatgtcctgc aatcctcagt gaagcatagc
     8401 cgtcagcgga tgtatctggt gccaccggtt ttttcacaac tgaccgaaca acatcaggct
     8461 gtcattgacg ccatttttgc tggtgatgcc gacggggcgc gtaaagcaat gatggcgcac
     8521 cttagttttg ttcacaccac catgaaacga ttcgatgaag atcaggctcg ccacgcacgg
     8581 attacccgcc tgcccggtga gcataatgag cattcgaggg agaaaaacgc atgattattt
     8641 ccgcagccag cgattatcgc gccgcagcgc aacgcattct gccgccgttc ctgttccact
     8701 atatggatgg tggtgcatat tctgaataca cgctgcgccg caacgtggaa gatttgtcag
     8761 aagtggcact gcgccagcgt attctgaaaa acatgtccga cttaagcctg gaaacgacgc
     8821 tgtttaatga gaaattgtcg atgccggtag cactggcacc ggtgggtttg tgtggcatgt
     8881 atgcgcgtcg tggcgaagtt caggcagcca aagcggcgga cgcgcatggc attccgttta
     8941 ctctctcgac ggtttccgtt tgcccgattg aagaagtcgc gccagccatc aagcgcccaa
     9001 tgtggttcca gctttatgta ctgcgcgatc gcggctttat gcgtaacgcg ctggagcgag
     9061 caaaagcagc gggttgttcg acgctggttt tcaccgtgga tatgccgaca ccgggcgcac
     9121 gctaccgtga tgcgcattca ggtatgagcg gcccaaatgc ggcaatgcgc cgctacttgc
     9181 aggcggtgac gcatccgcaa tgggcgtggg atgtgggcct gaacggtcgt ccacatgatt
     9241 taggtaatat ctcagcttat ctcggcaaac cgaccggact ggaagattac atcggctggc
     9301 tggggaataa cttcgatccg tccatctcat ggaaagacct tgagtggatc cgcgatttct
     9361 gggatggccc gatggtgatc aaagggatcc tcgatccgga agatgcgcgc gatgcagtac
     9421 gttttggtgc tgatggaatt gtggtttcta accacggtgg ccgccagctg gacggtgtac
     9481 tctcttccgc tcgtgcgttg cccgctattg cagatgcggt gaaaggtgat atcgccattc
     9541 tggcggatag cggaattcgt aacgggcttg atgtcgtgcg tatgattgcg ctcggtgccg
     9601 acaccgtact gctgggtcgt gctttcctgt atgcgctggc aacagcgggc caggcgggtg
     9661 tagctaacct gctaaatctg atcgaaaaag agatgaaagt ggcgatgacg ctgactggcg
     9721 cgaaatcgat cagtgaaatt acgcaagatt cgctggtgca ggggctgggt aaagagttgc
     9781 ctacggcact ggctccgatg gcgaaaggga atgcggcata gtcgtttgcg ccccctcacc
     9841 ctaaccctct ccctcaggga gaggggaccg ttcggcgctg tatgtactcc ctcactctga
     9901 aacgacaccg cactcttttt ttctccctcg cccctccggg gagggccggg gtgaggggaa
     9961 aaggccgcac ggttcaccaa ttttctgcta tcctgccccc gcactaaggg ggcagtatgc
    10021 taaacatcgt actttacgaa ccagaaattc cgccaaatac tggcaacatc atccgtcttt
    10081 gcgctaatac cggctttcgt ctacatatca tcgaaccgat gggatttgcc tgggacgaca
    10141 agcgcctgcg ccgcgcgggg ctggactatc acgagtttac cgccgttacg cgtcatcatg
    10201 actatcgcgc gttcctcgaa gcagaaaatc cccagcgcct gtttgccctc accacgaaag
    10261 gtacgcctgc tcacagcgcc gtaagctatc aggatggcga ttatctgatg ttcggcccgg
    10321 aaacacgcgg cctgccagcg agcattcttg atgccctgcc cgctgaacaa aaaattcgca
    10381 ttccgatggt gccggacagc cgcagcatga atctgtccaa tgcggtgtcg gtagtggtgt
    10441 atgaagcctg gcggcagctg ggatatccgg gagcgttgtt gagagattag ttactgtatg
    10501 ccggatagat gattacatcg catccggcac gatcacagga cattagatcc catccccata
    10561 ctcaaatgta tggttaatac cgttgaaatg ctggtccata tccattgatg gcttatcgct
    10621 gtctggttta ccgacaatac gagccggaac gccagcggcg gttgtatgcg gcggcaccgg
    10681 ttgcagcacc acggaacctg cgccaatctt cgcgccgcga ccaacttcaa tattgccgag
    10741 gattttcgcg cccgcgccaa tcatcacacc ttcacgaatt ttcgggtgac ggtcaccacc
    10801 agatttaccc gtaccgccaa gcgtcacaga ttgcagaatc gatacgtcgt tttcaattac
    10861 cgccgtttca cccacaacga tgcccgtggc gtggtcgagc ataatgccac ggccaatttt
    10921 tgctgccggg tgaatatcga cctggaacgt cacagaaacc tggttttgca gaaagattgc
    10981 caatgcgcga cgcccctgat tccacaacca gtgaccgatg cgataggcct gcaaggcatg
    11041 aaaacccttc aggtataaca acggggttga gtatttatcg actgccgggt cgcgggtacg
    11101 caccgcctga atatcacagg ccgcagaggc gatcatttcc gggtcagcgg cgtaggcttc
    11161 ttcaaccact tcacggatag caatagcagg cataattggc gatgacagct tgttcgccag
    11221 catgtagctc agtgcactgc caaggttttc gtgcttgagt agcgtcgcgt ggtaaaaact
    11281 ggccagcatt ggctcacagt ccgccagcgt tctggcttcg gctttaatat tgttccagac
    11341 aatttccagt tcttcacacg acattgctta ctccacacga tgagataatg accggcccgt
    11401 tctgcgcggg ccgggtcata gcggtaacaa aggttccctg gggttagtga ctgctgcgct
    11461 cgtccttgcg tgcacgacct aataatgtca atgctgcctc gcgcgcgttt tttccgcaat
    11521 ataatacttg ataaatttcc tcggttattg gcatttcaac gccgaagcga tgcgccagtt
    11581 cgcggacttc tttcgtattg cggtagcctt ccaccacctg accaatcttc tcctgcgcgc
    11641 tttgtacatc catgccctga ccgagcatca taccaaaacg gcggttacgc gactggttgt
    11701 cggtacaggt aagcaccaga tcgcccagcc ccgccatgcc cataaaggtg gcaggatcgg
    11761 cacccagcgc cgcaccaaga cgcgacattt cagccagccc acgggtgatc agcgccgtac
    11821 gcgcattcgc accaaaaccg ataccgtcag acatccccgc gccaatggca atgacgtttt
    11881 tcaccgcgcc gccaagctgc acgccaatga aatccgggtt gctgtaaacg cggaaacttt
    11941 tgccgcagtg cagcagctgc tggagatcat cggcaaaggt ctgatcggtc gaggccagcg
    12001 aaatagctgt cggtaaacct gccgccagtt ctttcgcaaa cgttgggcca gagataaccg
    12061 ccagcggaat ttgatcgcct aaggcctcac gcgccacgtc ctgtaacaga cgtccggttt
    12121 ccgcttccag ccctttggtc gcccacacca gacgcgcatc aggacgcatc agcggtttaa
    12181 tctggcgcag cacttcacca aagacatggc tgggtacgac gacgagaata ttacgactgg
    12241 ctgccagcgc agtggcgaga tcgctttcaa gatggagcgt atcgggaaaa ggcacatcgg
    12301 ggagaaacgc ggcgttacag cggtcgcgtt caagcgttgc gatatgttca gggtcatggc
    12361 cccagaggac aacctcgtgg ccatttcttg ccagggtaat ggcaagagcg gtgccgtacg
    12421 agccggcacc gatcacagtc attgaagcat tacgttggtt catcaggcat cctgatgttc
    12481 ttcagtacct tcgccagcct gctgctgcaa atagttcatg aacagcgcat cgaagttaac
    12541 cggcgcaagg ttcagttgcg ggaatgtacc gcgggatacc atgctggtga tgcactcacg
    12601 agcatacggg aacagaatgt tcgggcagta tgcgcccagg caatgtgcca tctgggtgcc
    12661 ttcgataccc gcgatggaga aaataccgcc ctgctgaact tcacacagga acgcggtttc
    12721 ttcgcccaag gaggccgtta cggtaacacg cagtaccact tcgtatacgt catctgccag
    12781 ttgggaagat gccgtatcca gatcaagttt aacttctggt tgccaatctt tctggaaaac
    12841 gtgcggcgcg ttcggcgctt cgaaagagat atccttggta taaatacgtt ggatctggaa
    12901 agtcatttca gtgttgtttt gttctgacat gtgtagaaaa cccttaagtg ttgtccttaa
    12961 atactgcgca atgtaatacc aacgttcaca cgttatttca gcaggggatc cagtccacca
    13021 cgtgcatcca atgcatacaa gtcatcacag ccgccaatgt gctgtgcgtc aataaaaatc
    13081 tggggaaccg tggtgcaacc gctgcgtttg atcatctctt cacgcttggc ggcattgcca
    13141 tcgatcggca actcctggaa actcacgccc ttgctgctca gcagtgcttt tgcacgatgg
    13201 caatacgggc aggtttcttt ggtatagatt tcaacattgg ccataattta gctcctgttt
    13261 ttttaccctg aaatttcatg ttgcagggcg gctgcaaaat ggagtgtccc cagaagctta
    13321 taacaataag tcgctggggg cctaattgca gctaacggcc tgcatcatga aagacgacag
    13381 gtaaattatt tgccgcgcac cagaggcaag ttttcgccag cccagccagc aacgccctct
    13441 ttcagcacga atacttgcgc aaaaccagct ttcgtcagcg cgtttgcagg ctcctggcac
    13501 tgcatgccag aaccgtctac cacgataacc gatttgtctt tgtgcttctc aagctcacca
    13561 acattgttgg ctttgatttc gctcggcaac agattgatag aacctgcgat atggccttta
    13621 cggaagtcat cacgctgacg taaatccaca accacagcgt cttctttgtt gatcagacgc
    13681 gtagcttcac cacgagtaat caccttcact ttcgaggtca ggctcttaaa cgtagtcaca
    13741 agaaccgcca ccagtaacgc gatccaggcg atactcagta tgggatgacg gccaacaaat
    13801 tgcataattt cttgcatggg gggtaacaac tcccgacgta gtgattaaaa aaaccaggaa
    13861 aggagtatac ctgcgcggtg cggcaaatac agccagcgcg ttaactggaa tgcaattttg
    13921 cggggcacga cgaaaaaaaa gcgcaaatct ggcatcggct tgccgcgagc ggtcgtatat
    13981 tttgatcttc agaggctatt ttatcgattc agctgtagta aaattacgca aattttgact
    14041 cttgagtatg aggttgtcgc aatgtcggtt tctaaaaaac ctatggtact ggtgattctg
    14101 gatggctatg gctatcgtga agaacagcag gataacgcca tttttagtgc taaaaccccg
    14161 gtaatggatg cactgtgggc caatcgtccg cataccctga tcgacgcttc cggtctggaa
    14221 gtcggtctgc ctgaccgtca gatgggtaac tccgaagtag gccacgttaa cctgggtgcc
    14281 ggccgcatcg tgtatcagga cctgactcgt ctggacgttg aaatcaaaga tcgcgctttc
    14341 tttgctaatc cagtgctggc tggcgcggta cataaagcga aaaacgcagg taaagcagta
    14401 cacattatgg gtctgctctc cgcaggcggc gtacacagcc acgaagatca catcatggcg
    14461 atggtagaac tggcagctga acgcggcgca gaaaaaatct acctgcacgc attccttgac
    14521 ggtcgcgaca ccccgccgcg cagtgctgaa tcctcgctga aaaaatttga agagaaattt
    14581 gctgcgctgg gtaaaggccg cgtagcgtcc atcattggtc gttactacgc gatggaccgc
    14641 gataaccgtt gggatcgcgt tgaaaaagcc tatgacctgc tgactctggc gcagggtgag
    14701 ttccaggccg atactgccgt tgctggtttg caggcagctt atgctcgcga cgaaaacgac
    14761 gagttcgtga aagcgaccgt tattcgtgca gaaggtcagc cagatgcggc aatggaagac
    14821 ggcgatgcgc tgattttcat gaacttccgt gctgaccgcg cgcgtgaaat cactcgtgca
    14881 ttcgtgaacg ctgatttcga tggcttcgcg cgtaagaaag tggttaacgt cgatttcgtg
    14941 atgctgaccg aatacgccgc tgacatcaaa actgcggttg cttacccacc cgcttccctg
    15001 gttaacacct tcggcgagtg gatggcgaaa aacgacaaaa ctcagttgcg tatttccgaa
    15061 accgaaaaat atgcccacgt tactttcttc ttcaacggtg gcgtagaaga gtcgttcaaa
    15121 ggcgaagatc gcattctgat caactcaccg aaagtggcta cctacgatct gcaaccggaa
    15181 atgagctccg cagagctgac cgaaaaactg gttgcggcca tcaagagcgg caaatacgac
    15241 accattatct gtaactatcc gaacggcgac atggtaggtc acaccggggt gatggaagcg
    15301 gcggttaaag cggttgaagc actggatcac tgcgtggaag aagtcgcgaa agcggttgag
    15361 tccgtgggtg gacaactgct gatcaccgct gaccacggta acgctgaaca gatgcgcgat
    15421 ccggcaacgg gtcaggcaca cacggcacac accaacctgc cagttccgct gatttacgtt
    15481 ggtgataaga acgtgaaagc ggttgcaggc ggcaaacttt ctgacatcgc gccgaccatg
    15541 ttgtcgctga tgggtatgga aatcccgcaa gagatgactg gtaagccgct gttcatcgtg
    15601 gaataatccc tccccatgag gggaaaggcg attaatacca tgacacgggc cgtgaaaccg
    15661 cgcaggtttg caatcaggcc catcatctac gccagcgttc ttagcgctgg cgtattgttg
    15721 tgcgcctttt ccgcccacgc ggatgagcgt gaccaactca aatctattca ggccgatatc
    15781 gccgcaaaag agcgcgcggt acgccaaaag caacaacaac gcgcaagcct gctcgcacaa
    15841 ttgaaaaagc aggaagaagc gatctctgaa gccacccgta agctgcgcga aacgcaaaac
    15901 acgctcaatc aactcaataa acagattgat gagatgaacg cgtcgattgc caaactggag
    15961 cagcaaaaag ccgcccagga gcgcagcctc gccgcacaac tggatgccgc attccgtcag
    16021 ggcgagcata ccggtattca gctaattctc agcggtgaag aaagccagcg tggacagcgt
    16081 ttacaggctt atttcggcta tctcaaccag gcgcgacaag aaaccattgc ccagttgaag
    16141 caaacgcgtg aagaagtcac catgcagcat gctgaactgg aagagaaaca aagcgagcaa
    16201 caaacgctgt tatatgaaca gcgcgcccaa caggcgaaac tgactcaggc tctgaacgag
    16261 cgtaaaaaga cgctggcggg gctggaatct tccatccagc aaggtcagca gcagctaagt
    16321 gaactgcgtg ccaacgaatc ccgtctgcgt aacagcattg cccgtgctga agccgcggcg
    16381 aaagcgcgtg ctgaacgaga agcgcgtgag gcccaggcgg ttcgcgagcg ccagaaagaa
    16441 gcgacgcgca aaggcaccac ctacaaaccg accgaaagcg aaaaatcgct gatgtcccga
    16501 actggtggcc tgggtgcgcc gcgtggtcag gcattctggc cggttcgcgg gccgacgctg
    16561 catcgctatg gcgagcagct acagggtgaa ttacgctgga aagggatggt tatcggtgcc
    16621 tctgaaggta ctgaagttaa agcaattgcc gacggtcgag tgattctggc tgactggctg
    16681 caaggttacg gtctggtggt ggtggttgag cacggtaaag gcgacatgag tctttacggc
    16741 tataatcaga gcgcactggt gagcgttggt tcgcaggttc gcgcgggcca gccaattgca
    16801 ctggtgggca gcagtggcgg tcagggtcgg ccttcactct atttcgaaat tcgccgccag
    16861 ggtcaggcgg tcaatccaca gccgtggttg ggaagataag ttttgtttcc atttcgtcgt
    16921 aacgttcttg catttgccgc tctgttggcg ctctcctccc ccgtacttgc tggcaaactt
    16981 gccatcgtca ttgatgattt tgggtatcgc ccgcacaacg aaaaccaggt gctggcgatg
    17041 ccttccgcta tctccgtcgc tgtattaccc gattcaccgc acgccagaga gatggcgacc
    17101 aaagcgcata acagcggtca cgaagtgttg attcatctcc cgatggcacc gttgagtaaa
    17161 cagccgctgg agaaaaatac gctacgcccg gagatgagca gcgacgaaat tgagcgcatt
    17221 attcgtagtg cggtcaataa cgtgccctat gccgtgggga tcaacaacca catgggcagc
    17281 aagatgacct ctaacctgtt tggtatgcag aaagtgatgc aggcgctgga gcgttacaat
    17341 ctttacttcc tcgacagcgt aaccatcggt aatacccagg cgatgcgcgc cgcgcaaggt
    17401 actggcgtga aggtgatcaa acggaaggtg ttcctcgacg attcgcagaa cgaagcggac
    17461 atccgtgtgc aatttaatcg cgcaattgat ctggcgcgtc gcaacggttc aaccattgcc
    17521 attgggcatc ctcacccttc aacggtacgc gtgctacaac agatggttta taacctgccg
    17581 ccagacatta cgctggtgaa agccagcagc ttgctgaatg aaccgcaggt tgatacttct
    17641 acaccgccga aaaacgctgt gcctgatgca ccgcgtaatc cgttccgtgg cgtgaagctg
    17701 tgcaaaccga agaaaccgat agaacctgtt tatgctaatc gcttctttga agtattaa
//
LOCUS       DAJTUI010000079        17666 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTUI010000079 DAJTUI010000000
VERSION     DAJTUI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.079"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            37..774
                     /locus_tag="OO464_002908"
     CDS             37..774
                     /locus_tag="OO464_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6227984.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            885..1895
                     /gene="pyrD"
                     /locus_tag="OO464_002909"
     CDS             885..1895
                     /gene="pyrD"
                     /locus_tag="OO464_002909"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6227985.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            2069..2611
                     /gene="zapC"
                     /locus_tag="OO464_002910"
     CDS             2069..2611
                     /gene="zapC"
                     /locus_tag="OO464_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6227986.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(2608..3717)
                     /gene="ycbX"
                     /locus_tag="OO464_002911"
     CDS             complement(2608..3717)
                     /gene="ycbX"
                     /locus_tag="OO464_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6227987.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            3961..6069
                     /gene="rlmKL"
                     /locus_tag="OO464_002912"
     CDS             3961..6069
                     /gene="rlmKL"
                     /locus_tag="OO464_002912"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6227988.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            6081..7988
                     /locus_tag="OO464_002913"
     CDS             6081..7988
                     /locus_tag="OO464_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6227989.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            8118..9371
                     /gene="pqiA"
                     /locus_tag="OO464_002914"
     CDS             8118..9371
                     /gene="pqiA"
                     /locus_tag="OO464_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6227990.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            9376..11016
                     /gene="pqiB"
                     /locus_tag="OO464_002915"
     CDS             9376..11016
                     /gene="pqiB"
                     /locus_tag="OO464_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6227991.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            11013..11576
                     /gene="pqiC"
                     /locus_tag="OO464_002916"
     CDS             11013..11576
                     /gene="pqiC"
                     /locus_tag="OO464_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6227992.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            11832..11999
                     /gene="rmf"
                     /locus_tag="OO464_002917"
     CDS             11832..11999
                     /gene="rmf"
                     /locus_tag="OO464_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6227993.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(12069..12587)
                     /gene="fabA"
                     /locus_tag="OO464_002918"
     CDS             complement(12069..12587)
                     /gene="fabA"
                     /locus_tag="OO464_002918"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6227994.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(12656..14416)
                     /locus_tag="OO464_002919"
     CDS             complement(12656..14416)
                     /locus_tag="OO464_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6227995.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            14601..15053
                     /gene="matP"
                     /locus_tag="OO464_002920"
     CDS             14601..15053
                     /gene="matP"
                     /locus_tag="OO464_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6227996.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(15129..16175)
                     /gene="ompA"
                     /locus_tag="OO464_002921"
     CDS             complement(15129..16175)
                     /gene="ompA"
                     /locus_tag="OO464_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6227997.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(16531..17040)
                     /gene="sulA"
                     /locus_tag="OO464_002922"
     CDS             complement(16531..17040)
                     /gene="sulA"
                     /locus_tag="OO464_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6227998.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            17259..>17666
                     /gene="sxy"
                     /locus_tag="OO464_002923"
     CDS             17259..>17666
                     /gene="sxy"
                     /locus_tag="OO464_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR6227999.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
BASE COUNT         4463 a         4212 c         4643 g         4348 t
ORIGIN      
        1 gacctgtcaa tagcgatgtg cagtcgggta aagtgaatgc cagcgcgaca tttgtacttc
       61 attatgacta atacgtggaa cagattagcg ctcttgattt tcgccgtttt atcgctgctg
      121 gtggcgggtg aattgcaggc aggagtggtg gtcggcggaa cgcgatttat ctttccggca
      181 gacagagaat cgatatctat tttactgact aataccagtc aggaatcttg gcttattaac
      241 agcaaaatca atcgcccaac gcgttgggcc gggggggaag cgtcgacagt gccagcacta
      301 ttactggccg ctccgccact tattctcctg aagcccggta ctacaggcac gttgcgcttg
      361 ctgagaacgg aaagcgacat cttgcctgtg gatcgcgaaa cgctatttga gttaagcatt
      421 gccagcgtgc catccggcaa agttgaaaat cagagcgtaa aagtggcgat gcgctcggta
      481 tttaaactgt tctggcgacc cgaaggtttg ccgggcgacc cgctggaagc ttatcaacaa
      541 ttacgctgga cacggaattc gcagggtgta caactcacta acccaacgcc ttactacatt
      601 aacctgattc aggtgagcgt aaatggtaaa gcgttaagta atgcgggagt cgtgccgcct
      661 aaaagccagc gtcagacaaa ctggtgtcag gtaatagcac cgtgtcatgt cgcctggcgg
      721 acgataaatg attacggcgg gttgagtgca aagaaggagc aaaatctgcc ctgaaacagg
      781 ttctgaaaac gtttgcgttt tttttgccgc aggtcaattc ccttttggtc cgaactcgca
      841 cataatacgc ccccggtttg cacaccggga atccaggaga gttcatgtac taccccttcg
      901 ttcgtaaagc ccttttccag ctcgatccag agcgcgctca tgagtttact tttcagcaat
      961 tacgccgcat taccggaaca ccgtttgaag cactggtgcg gcagaaagtg cctgcgaaac
     1021 ctgttaactg catgggcctg acgtttaaaa atccgcttgg tctggcagcc ggtcttgata
     1081 aagacgggga gtgcattgat gcgttaggcg cgatgggatt tggatcgatc gagatcggta
     1141 ccgtcacgcc acgtccacag cctggtaatg acaagccgcg tctctttcgt ctggtagatg
     1201 ccgaaggttt gatcaaccgt atgggcttta ataatcttgg cgttgataac ctcgtagaga
     1261 acgtaaaaaa ggcccattat gacggcgtct tgggtattaa tatcggcaaa aataaagata
     1321 cgccagtgga gcagggcaaa gatgactatc tgatttgtat ggaaaaaatc tatgcctatg
     1381 cgggatatat cgccatcaat atttcatcgc cgaatacccc aggattacgc acgctgcaat
     1441 atggtgaagc gctggatgat ctcttaaccg cgattaaaaa taagcaaaat gatttgcaag
     1501 cgatgcacca taaatatgtg ccgatcgcag tgaagatcgc gccggatctt tctgaagacg
     1561 aattgatcca ggttgccgat agtttagttc gccataatat tgatggcgtt attgcaacca
     1621 ataccactct cgatcgttct ctggttcagg gaatgaaaaa ttgcgatcaa accggtggct
     1681 taagtggtcg tccgcttcag ttaaaaagca cagaaattat tcgccgcttg tcactagaat
     1741 taaacggtcg cttaccgatc atcggtgttg gcggcatcga ctcggttatc gctgcgcgtg
     1801 aaaagattgc tgcgggtgcc tcactggtgc aaatttattc tggttttatt tttaaaggtc
     1861 cgccgctgat taaagaaatc gttacccata tctaattatt tcttcgaatt cctctttata
     1921 agataatcag ggctttattt tcagacctgg ttgttttata ttcatcgttg ttgcttattt
     1981 agacattttg tacttttatt gttgaggtta ttaagcgaag cgacaatgga ttgtgttgct
     2041 gcggttttat agggtagggg agaggcagat gcgaattaaa ccagacgata actggcgttg
     2101 gtattacgat gaagagcacg atcgaatgat gctcgattta gccaatggta tgctatttcg
     2161 ctcacgtttt gcgcgcaaaa tgttgactcc agatgccttt tctcccgcag gcttttgtgt
     2221 tgatgatgcc gcgctctatt tctcttttga agaaaagtgc cgcgatttta atttatctaa
     2281 agaccaaaaa gccgaactgg tgctgaatgc actggtagca attcgttatc tcaaaccgca
     2341 aatgccgaaa agctggcatt ttgtctccca tggtgaaatg tgggtgccca tgccgggcga
     2401 tgcggcctgc gtttggttga gcgatactca cgagcaggtc aatttgctgg tcgttgaatc
     2461 tggcgaaaat gccgcgctat gcctgctggc acaaccctgc gttgttattg cgggtcgcgc
     2521 gatgcagttg ggcgacgcca ttaaaatcat gaacgacagg ctgaaaccgc aggtgaatgt
     2581 tgacagcttc agcctcgaac aggcagtcta acgcgtcagc ttaagggcag tcttcggtac
     2641 acagctacag caaagaatgg tgccatcatc gcccattgct gattttttca gcggtgtgac
     2701 ttcgccttct aaaagctgaa cacggcaact tccacaaatg cccgcgcggc aagaataagg
     2761 gatacgaatt ccctgatttt ctaattgctc cagcaacact tgttggttat ttccacgaaa
     2821 tgcctgtccc tgccagtcaa tatctacatt tgcgtccggt tgttgcgtga tgttggcggt
     2881 atcatcagcg gcagctgcgc cgtaaatttt tgccggagcc gttgccagaa tttccacctc
     2941 atcgccaacg cgaatcacgc cgctattacg ggcaattaaa ttctgaccaa aatcgacatc
     3001 gccgttatcc tgggcagtgc ggaaagattg caatgttttt aatggttcgc ctgccggatg
     3061 tttttgccct ttttctgggc tgacggtggt gaaaatacag cggctacaag gtttaaccac
     3121 atcaaacacc acatcaccaa tgcgaatcac tttccagcta tcttcttccc aggctgacgc
     3181 gccggaaacc accagattgg ggcggaattg ctccattttt acactggccg gacaacgttg
     3241 ttggagatca cgtaacgagg cttcgttagc aagaaggtaa ggatagccat cagcaaatga
     3301 cagaggtaca gtgttgtggc gtttcacgcg ccgggtcatt tgtggcccca cccagcgtaa
     3361 ttgcacttcg cgggagaaaa atccacttag ccatttgttg atcgcgtctg gcgcaattcg
     3421 cgcggtaaaa tgtgtgcccc aaacttcggt tggtgcgtct tgtgtggcga aatcagcaaa
     3481 acgaacatat gcgctactgc catctggtgc ggtgagatgc aagccatcat gcacaggtga
     3541 aggagtaaac cgtaccatct ggggaaactg gcgagcggta ataaacgtac cgtcaggttc
     3601 cgtgatcata aagatgcgat cgaaggccag accactgaca tcagccagag catgtgtaag
     3661 accaatgccg cgcatcgatt taacaggatg aataaaaagc cgggttaatg tcgccacggt
     3721 gcggtcctca aatgaaaata agccctcaac tttatgacat gacgcgctta ttagctataa
     3781 tgcgcaacaa ttttcttagc gcctgtcccg ttaggctatt ttatttgtca gtttggccct
     3841 gggttgtgct cgaaatcctc acgtacttgt gtacgctccg gtttctccgc gctctccatg
     3901 tccagactga ctgcaacaat tacgcctact gcggtaggct cttagagtaa aagtgacgat
     3961 atgaattctc tgtttgccag tacggcccgt gggctggaag agctgttaaa aactgaactg
     4021 gaaaatctgg gggccgttga atgccaggtg gttcagggtg gggtccattt caagggcgac
     4081 acacggcttg tttaccagag cctgatgtgg agccgcctgg cctcgcgtat tatgttgccg
     4141 ctgggcgagt gtaaggttta cagcgattta gacctctatc tcggtgttca ggcgatcaac
     4201 tggacagaga tgtttaatcc tggcgcgacc ttcgctgtcc acttcagtgg tttgaatgac
     4261 accatccgca acagtcagta cggcgcgatg aaagtgaaag acgcgatcgt cgatgctttc
     4321 acacggaaaa atctgccgcg tccaaatgtt gatcgcgatg cgccggatat ccgcgttaac
     4381 gtctggctgc ataaagaaac cgccagtatc gctcttgatc tgagtggtga tggtttacat
     4441 ctgcgtggct atcgcgatcg tgctggtatt gcgccgatca aagaaaccct ggcagccgct
     4501 attgtgatgc gatccggctg gcagtcagga acaccgctgc tcgatccgat gtgtggttcc
     4561 ggtacgttgc tgattgaagc ggcgatgctg gcgaccgatc gcgcaccagg cttgcaccgt
     4621 gggcgttggg gctttagcgg ctgggcgcag catgatgaag ctatctggca ggaagtgaaa
     4681 gcggaagcgc aaactcgcgc ccgtaaaggc ctagctgagt atagctctca tttttacggt
     4741 tcggacagcg acgcacgggt gattcaacgt gcgcgcacta acgcccgtct tgcggggatt
     4801 ggtgaactga tcacctttga ggtgaaagat gtcgcgcaac tgaccaatcc gctgccgaaa
     4861 gggccgtacg gtacagtgtt gagcaacccg ccatacggtg aacgtctgga cagcgaaccg
     4921 gcgctgattg cgctgcatag tctgctgggc cgaatcatga aaaaccagtt cggtggctgg
     4981 aatctctctt tgtttagtgc ctcgccggat ctgctaagct gcctgcagct gcgtgcagac
     5041 aaacagtaca aggcgaaaaa cggcccgctg gactgcgtac agaaaaatta ccacgttgcc
     5101 gaaagcacac cagacagtaa accggcaatg gtagcggaag attacgccaa ccgcctgcgt
     5161 aagaacctca aaaaattcga gaagtgggca cgccaggaag ggattgaatg ttaccgcctg
     5221 tatgacgccg atctgccgga atataacgtt gccgttgacc gttatgccga ctgggtggtg
     5281 gtgcaggagt atgcgccgcc aaaaactatt gatgcgcaca aagcgcgtca gcgtctgttc
     5341 gatattatcg ctgcaaccat ttcggtactg gggattgcgc caaacaaact ggtgctgaaa
     5401 acccgtgaac gccataaggg caaaaaccag taccagaaac tgggcgagaa gggcgagttt
     5461 cttgaagtta ccgaatataa cgctcacttg tgggtgaacc tgacggatta tctcgatact
     5521 ggtctgttcc tcgatcaccg tatcgcccgc cgtatgctcg gtcagatgag caaaggcaaa
     5581 gatttcctca acctgttttc ttataccggc agcgccaccg tgcatgcggg attaggcggt
     5641 gcacgcagca ccaccaccgt ggatatgtcg cgtacttatc tggaatgggc agaacgcaac
     5701 ctgcgtctga atggcctgac taggcgtgcg catcgcctga ttcaggccga ttgcctggcg
     5761 tggctgcgtg aggcaaatga acagttcgat ctgatcttta tcgatccgcc aaccttctct
     5821 aactcaaaac gaatggaaga tgcgtttgat gttcagcgcg atcatctggc gctgatgaaa
     5881 gatttgaaac gtctgctgcg tgcaggtggg acgatcatgt tctcgaacaa caaacgtggc
     5941 ttccgtatgg atctcgacgg cctggcaaaa ctgggactga aagcacaaga aattacgcaa
     6001 aaaacgctct cccaggattt cgcccgtaac cgccagatcc acaactgctg gctgattacc
     6061 gcagcctgaa aggaatagta atgtcattaa tcagtatgca tggcgcatgg ctgtcgttca
     6121 gcgacgcgcc gcttctcgat aacgcagaac tgcatatcga agataacgaa cgtgtttgtc
     6181 tggtgggccg caacggcgca ggaaaatcga cgttaatgaa aatcctcaac cgtgaacaag
     6241 ggctggatga cggtcgcatt atttatgagc aagatttgat tgtagctcgt ctgcaacagg
     6301 atccgccgcg taacgttgag ggtagcgttt atgatttcgt tgccgaaggc attgaagaac
     6361 aagcggaata tctgaaacgc tatcacgata tttcgcgcct ggtgatgaac gacccgagcg
     6421 agaaaaatct caacgaactg gcgaaggttc aggaacagct ggatcaccac aacctgtggc
     6481 agctggaaaa ccgcatcaac gaagtgctgg cgcaactggg gttagatcct aacgttgcgc
     6541 tgtcgtcgct ttccggcggc tggttgcgta aagcggcatt aggacgcgcg ctggtgagca
     6601 atccgcgcgt attgctgctg gacgaaccga ctaaccatct ggatatcgaa accatcgact
     6661 ggctggaggg gttccttaaa accttcaatg ggacgattat tttcatctcc cacgaccgtt
     6721 cgtttatccg caatatggcg acgcgcattg ttgatctcga tcgcggcaag ctggtgacct
     6781 atccagggaa ttacgaccag tacctgcagg aaaaagaaga agccctgcgc gtggaagaac
     6841 tacaaaatgc cgagttcgat ctcaaactgg cgcaggaaga agtgtggatt cgccagggga
     6901 tcaaagcacg ccgtacccgt aatgaaggcc gcgtacgcgc cctgaaagcg atgcgtcgcg
     6961 aacgtggtga acgtcgcgaa gtgatgggta ccgcaaagat gcaggtggaa gaggccagcc
     7021 gctccggtaa gatcgttttc gaaatggaag acgtttgcta ccaggttaac ggtaagcaac
     7081 tggtgaaaga tttttctgcc caggttctac gtggcgacaa aattgccctg attggtccga
     7141 atgggtgcgg caaaaccacg ctgctgaaac tgatgctcgg tcagcttcaa gcggacagcg
     7201 ggcgtattca cgttggcacc aaactggaag tggcttattt cgatcagcac cgcgcggaac
     7261 tggatcccga taaaacggtg atggataacc ttgccgaagg taagcaagag gtgatggtta
     7321 atggcaagcc acgccacgta ttgggctatt tgcaggactt tctgttccat ccgaaacggg
     7381 cgatgacgcc agtacgtgcg ttttctggcg gtgagcggaa ccgcttgctg ctggcgcgtt
     7441 tgttcctcaa accaagcaac ttattgattc ttgacgaacc gaccaacgat cttgatgtcg
     7501 aaacgctgga actgctggaa gaactgatcg acagctatca gggcacggta ttgctggtta
     7561 gccacgatcg tcagtttgtc gataacaccg ttacagaatg ctggatcttc gaaggcggcg
     7621 gtaaaattgg tcgttatgtt ggcggttatc atgatgcccg tggtcagcaa gaacaatatg
     7681 tggcactcaa acagcctgcg gtgaaaaaaa gcgaagaagc cgccgcgcca aaagcagaaa
     7741 ctgtaaaacg cagcagtagc aaactaagct ataaattgca gcgcgaactg gagcagctac
     7801 cgcaattgct cgaagatctg gaggcgaagc tggaagccct acagacgcaa gtggcggatg
     7861 cttccttctt cagtcagccg catgagcaga cgcaaaaagt gcttgctgat atggctgctg
     7921 cagagcagga gctggagcaa gcctttgaac gctgggagta tcttgaagcg ttaaaaaatg
     7981 gtggctgatc gcaaaatagt cgatttaaac agagacagct gacaacggta aaattgcctg
     8041 atgaactggg tttatcaggc atgaaaacca acactttgtt agtcatctgt aaaatagcgc
     8101 atcattaagg agtaccaatg tgcgaacatc atcatgccgc gaagcacatc ctgtgctcgc
     8161 agtgtgacat gctggtggcg ttaccgcgcc ttgagcatgg tcagaaagcg gcatgtcccc
     8221 ggtgtggcac aacgttaacc gtggcgtggg atgcccctcg gcagcgtccc accgcctatg
     8281 cgttggctgc actgttcatg ctgttgctgt ccaacttgtt tccttttgtg aatatgaacg
     8341 ttgcaggagt taccagtgaa attacattac tggaaattcc cggcgtgctt ttttctgagg
     8401 actacgccag cctcggcacc tttttcctgt tgtttgtgca actggttccc gcgttttgtc
     8461 tgataaccat tctgttactg gtgaatcgcg cggaattacc ggtccgttta aaagagcaac
     8521 tggcacgggt gctttttcaa ctcaaaacct ggggaatggc ggagattttc ctcgcgggtg
     8581 tgctggtcag tttcgttaaa ctgatggctt acggcagcat tggggtaggc agcagctttc
     8641 tcccctggtg tttattttgt gtcctgcaac tgcgcgcttt tcagtgcgtt gatcgtcgct
     8701 ggttatggga cgacatcgcc ccgatgccag aactgcgcca gccgctaaaa ccaggcgtca
     8761 cggggatacg tcaggggctg cgttcgtgct cctgttgtac ggcaatcctt cctgctgatg
     8821 aacccgtgtg cccgcgttgt agtaccaaag ggtacgttcg gcgtagaaac agcctgcagt
     8881 ggacactcgc gctgcttgta acgtccatca tgctgtatct tccggctaat attttgccca
     8941 tcatggtgac ggatttatta ggctcgaaga tgccgtcgac gattctcgct ggggtcattc
     9001 tgttatggag cgaaggctct tatcccgtcg ctgcggtgat ctttctggcc agtattatgg
     9061 tgccaacgtt aaagatgatc gccatcgcgt ggctgtgttg ggatgccaaa gggcatggca
     9121 agcgcgacag tgaaagaatg catttgattt atgaagttgt tgagtttgta ggccgctggt
     9181 cgatgattga cgttttcgtt atcgcggtgc tctcggcgct ggtgcgtatg ggaggtttaa
     9241 tgagtattta tccggcaatg ggtgcattaa tgtttgcttt agtcgtcata atgacaatgt
     9301 tttctgctat gacgtttgac ccgcgtttgt cgtgggatcg tcaacctgaa tcagagcatg
     9361 aggagtcctg acagtatgga atctaataat ggggaagcca aaatccagaa agtgaagaac
     9421 tggtctcccg tgtggatatt tcctatcgtc acggcgctca ttggggcctg ggttcttttt
     9481 tatcattaca gccatcaggg accggaagtg accctgatca ccgcgaatgc ggaaggaatt
     9541 gaaggtggca aaaccaccat taaaagccgt agcgttgacg tcggcgtggt tgaaagcgcc
     9601 acactggctg atgatttgac gcacgttgaa atcaaagcgc ggctgaattc cggtatggaa
     9661 aaattgctgc ataaagacac cgtcttttgg gtggtgaaac cgcagattgg tcgcgaaggg
     9721 attagcggcc tgggaacgct gctgtctgga gtttatatcg aactgcagcc aggcgcgaaa
     9781 ggcagcaaaa tggataaata cgatttgctg gactcaccac cgttggcccc gcctgatgcg
     9841 aaaggtatcc gtgtgattct cgatagcaaa aaagccgggc agctctcgcc aggagatccg
     9901 gtgctgttcc gtggctatcg ggtaggttcg gttgaaacca gcaccttcga tacgcaaaaa
     9961 cgcaatatca gttatcaact gttcatcaat gcaccttatg accgactggt gaccagcaat
    10021 gttcgcttct ggaaagatag tggcattgcg gttgatctga cgtcagcggg gatgcgtgtg
    10081 gagatgggct cattgacaac gctgcttagt ggcggtgtta gctttgatgt gccggaaggt
    10141 ctggatttag ggcagccagt ggcaccgaaa acagctttcg ttttgtatga tgatcagaag
    10201 agcattcagg attcgttgta caccgatcac attgattatc tgatgttctt taaagattcg
    10261 gtacgcggtc tgcaaccggg agctccggta gaattccggg gtattcgcct gggtaccgta
    10321 agcaaagtgc cattctttgc gccgaatatg cgtcagacat ttaacgatga ttaccgtatt
    10381 ccggtactga ttcgtatcga gccagagcgg ctgaaaatgc agcttggcga aaatgcggat
    10441 gttgttgagc accttggcga attgttgaaa cgtggtttac gcggatcgct gaaaaccgga
    10501 aacctggtca ctggtgcact gtatgttgat ctcgatttct atccaaatac gcctgcaata
    10561 accggtattc gtgaatttaa tggttatcag attatcccga ctgttagcgg cggcttggcg
    10621 caaatccagc aacgactgat ggaagcgttg gataagatca acaaactgcc attgaatccg
    10681 atgattgaac aggcaaccag tacgctttct gaaagtcagc gcacaatgaa aaacctgcaa
    10741 acgacgctgg atagcatgaa caagatcctc gccagccagt cgatgcagca gttgccgacg
    10801 gatatgcagt caacgttgcg tgaattgaat cgcagcatgc agggcttcca gcctggctcc
    10861 gcagcctaca acaagatggt ggcggatatg cagcgccttg atcaggtgtt gcgagaactg
    10921 caaccggtgc tgaaaacgct caatgagaag agtaacgcgc tggtatttga agcgaaggac
    10981 aaaaaagatc cagagccgaa gagggcgaaa caatgaaaaa gtggctagtg acgattgcag
    11041 cactgtggct ggccggatgc agctccggcg aaattaataa aaactattac cagttacctg
    11101 tggtgcagag cggtacacaa agtaccgcca gccagggcaa tcgtctgtta tgggtagagc
    11161 aggtcactgt tcctgactat ctggcgggga atggtgtggt ttatcaaacc agtgatgtga
    11221 agtatgtgat tgccaacaac aacttgtggg ccagcccgtt ggatcaacag ttgcgcaaca
    11281 ccctggttgc caacctgagt acgcaactgc ccggctgggt ggttgcctcc cagcctctgg
    11341 gaagcgccca ggacacgctc aatgttaccg taacggagtt taacggtcgc tatgatggca
    11401 aggtcattgt cagtggtgag tggctgttga accaccaggg acaactgatc aaacgtccgt
    11461 tccgtctgga aggagtgcaa actcaggatg gttacgatga gatggttaaa gtgctggccg
    11521 gtgtctggag tcaggaagcc gcttctattg cacaagagat aaagcgtcta ccttaattat
    11581 aaagatttgt aaatataacc gtctccggta tgttgcctga ggcggttttt ttgtttctaa
    11641 cgtgcggaaa aatttgttcc tcttcacatt ttttgtacaa ccgacatgcc cgtgtagctc
    11701 acaaatatga cagtggcgtg aattttgcgc attgacggca gttatgattc gcggtattgc
    11761 ttaactgtga ttgcacattt agtaatcact gttttctttt ccaccagaaa ccagtatgag
    11821 ggaaacgagg catgaagaga caaaaacgag atcgcctgga acgggcacat caacgtggtt
    11881 atcaggccgg catcgccgga cgctcaaaag aaatgtgtcc ctatcagacg ctgagtcaaa
    11941 ggtcacaatg gctgggaggc tggcgagaag ccatggcgga cagggtagta atggcctgat
    12001 tctgtctctt taaaaagaaa cctccgcatt gcggaggttt cgccttttga tactctgtct
    12061 gatgataatc agaaggcaga cgtatcctgg aacagaccga ctttcaggtc gctggcggta
    12121 tagatcagac gacaatcaac cagcacttcg ccatctgcca gacccataat cagacgacga
    12181 ttaacaatgc gtttaaagtg aatacggtag gtcacttttt tcgctgtcgg cagtacctga
    12241 ccagtgaatt tcacttcgcc aacgcccagc gcgcggcctt taccttcgcc gcccagccag
    12301 ccgaggtaga accctaccag ctgccacatt gcgtccaggc ccaggcatcc cggcataacc
    12361 ggatcgccaa tgaagtggca tccgaagaac cacagatccg gattgatatc cagttctgct
    12421 tcaacatacc ctttgtcgaa gttaccaccc gtttcggtca ttttgaccac acggtctatc
    12481 atcagcatgt tcggtgctgg caattgcggg cctttagcgc caaacagttc accgtgacca
    12541 gaggcaagaa ggtcttcttt tgtataggat tcgcgtttat ctaccatgtt ctctgtaagc
    12601 cttattttat tgaagcacgc aggatagcta acacgtgtac gctgaacaag tccgatcagt
    12661 tcggaataaa ccagttcagc caacgtaatg gccatggaaa acggtgacgt ccttcctgtt
    12721 gcgatgcttg cgcgatacgt tcctggatgg tttgcatcag cgtcgtttgg ccttcgccat
    12781 cccacaccag atttaataat aacggcagtg cgtcagtcac atcgtctact gcccagatgg
    12841 tgaatttgcc ttcttctacc gctttcacca gttcactgtg aagacttaaa tggcgaacgt
    12901 tagcagtggg gataatgaca ccttgtttcc ccgttaactc acgttgctgg caaatagcaa
    12961 agaagccttc gattttctca tttaaaccac cgaccggctg gacgcggccg aactgatcga
    13021 ctgaacctgt gatagcgata ctctgattca ctggcacatc agcgagggcg cttatcaggg
    13081 cgcagagttc agccatcgag gcactatcgc catccacttc actgtatgac tgctcaaatg
    13141 tcagcgatgc tgagaagggg atctgttgct caagctgtag ttccgacatc aggaacgcct
    13201 gcatgatcat catccctttc gcatggatat tgccgccaag ctccgctttg cgttcgatgt
    13261 cggtgaattc accatcgcca atatgcacaa cgcagctaat gcgagaaggt tcgccaaaag
    13321 cgcgtggatg acccggaaat tcaatgaccg aaagggcgtt aatttgcccg atgcgttcgc
    13381 cttcggtttc aatcaggatt tgctcctgaa ggatctcatc ctgcatacgt tcagcgagga
    13441 aaccttcgcg ccattcacgc tgctgcagca ttaagtttag ctgctcgccg gagaaggtgt
    13501 cgccatcaca cagggaggcg acctctttac actggcggag gatccactgc gggctaagcg
    13561 gaagtgtttc ttgttcaccg gtgtagcgtg ctgcttcgcg gataagtacc ggccaagcat
    13621 ccgctcccgg ggcaggtaag tgattatgtc tggcggtaaa tgtcacccag cgacaccact
    13681 ggcttactga ctccgcatcg acaatctgca gagtatcttc aaattcgcta taaatagcct
    13741 gctctgaaag ctctggctcc atctcctgga aatcagccaa tgattcgcgt tcgcctacca
    13801 gaatgacttt cagcttcaat ggcattgaag gcacagagac ggggagaggg cgcgactcat
    13861 caaacgcaac ccagtcaaaa cgctcgcggt taacgatatt tttcagccgc atccacagca
    13921 gaggttgcgc cagcagtgta cgcaaagaga tgatgagaat accgccattt gcctgatgca
    13981 ccagaccagg ctgcagggta atgtcgccat taaactggcg caggcagcca aagagttgct
    14041 ccgcttctac ccagtcggca gcgacaactt gcgttaaagt cgcaaaatta tcatctgcac
    14101 tcactgcgtg acgtaagcgg atggagtggc cggaaacctc atagtgatcg cccaccagtt
    14161 ggcctgcatc gctttgtagc gtacgcgcgg catcggcaat aagattcaga tactcagact
    14221 cttccggggc cttagccagc ataaaagagg aggatgctcg cgtatgcagc aactgctcca
    14281 gcgcaaattg cagtcgcggt tgagtatcac tgaataaagg atcgttttcg tcaatcaaat
    14341 gtggctgagc aaatatttcc tgatagctat cggtatcagg aaccaggtca cgccatgcaa
    14401 gtttcgtaat ggtcaaagtt gatgtttttt agtctgttgt caaagccgcg attataccgt
    14461 aaccggcact acagcacacg tagaaagcac cgacaatact cctggctggg cgttaaagct
    14521 cacaggatgg agattctttt cttcactggc ctaaaaagct gatattctgt aaagagttac
    14581 acggtaacat tgagatcgct atgaaatatc aacaacttga aaatcttgaa agcggttgga
    14641 aatggaagta tctggtgaag aagcatcgcg aaggggagtt aattacccgt tacatagaag
    14701 ccagtgccgc ccaggaagcc gtcgatgtgt tgctctcgct cgaaaatgaa cccgtgctgg
    14761 taaatggctg gattgacaag catatgaatc cggaactggt caatcgcatg aagcagacca
    14821 ttcgggccag acggaagcgc cattttaatg cggaacacca gcatacgcgc aaaaaatcga
    14881 tcgatctgga atttattgtc tggcaacgtc tggctggtct tgcacagcgt cgcggtaaaa
    14941 cgctttctga aacgattgtt cagctgattg aagatgcgga aaacaaagag aaatacgcga
    15001 ataaaatgtc ttctcttaag caggatctgc aggcattgct gggtaaggaa taactgacga
    15061 aagtcagttc aatttactaa aggcaaaaaa aaccccgcag cagcggggtt tttctaccag
    15121 acgataactt aagcctgcgg ctgagttaca acgtctttga tacctttaac ttcgatctct
    15181 acgcgacgat ccggagccaa gcagtcgatc agtgcagcac gctgtttcac gttgtcacag
    15241 gtgttgccag taaccgggtt ggattcgccc ataccacgtg cggagatctt gtctgccggg
    15301 atacctttgg agatcaggta atcaacaaca gactgagcac ggcgctcgga cagaccctgg
    15361 ttgtaagcgt cagaaccgat gcggtcagtg taacccagaa caactacgga accgtctttc
    15421 ggatctaggt tgctcagctg gctgtacagc tgatccagag cagcctgacc ttccggtttc
    15481 agggttgctt tgttgaagtt gaacagaacg tcagacttca gagtgaagtg cttggtctgt
    15541 acttccggtg ccggagctgg agcaactacc ggagctgctt cgccctgacc gaaacggtag
    15601 gaaacaccca ggctcagcag accgttgtcc ggacgagtac cgatggtgtt tgcgtcaccg
    15661 atgttgttgg tccactggta ttccagacgg gtagcgattt caggagtgat cgcgtactca
    15721 acaccacctg cgaaaaccgg agaaacgcca gtgtcgtggt ctttaaagga tgcgccacca
    15781 ggtacgttag ccttggtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc
    15841 agatcgtcag tgattgggta acccagttta gcggtcagct gaacgccctg agctttgtat
    15901 gcgccgttga tgttgtcgcc tttgtacggc atacgaccta accagtcgta acccatttca
    15961 aaaccaacat acgggttaac ctggtaacca ccaaaagcac ctgcacccag ttggttttcg
    16021 tgggtcggac cattgttagg aataaaacca gtgtcatggt actgggacca gcccagttta
    16081 gcaccagtgt accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc
    16141 agtgccactg caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa
    16201 atacgccatg aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct
    16261 caatattaac tctaccgata tcttcggctt atgccgagca cccctggcga tgtaaagtct
    16321 acaacgtagt tgaaaactta caagtgtgaa ctccgtcagg catatgaaaa aaaagtcttg
    16381 tataaggtat gtttaatctt tttgtcagcg acaatttaca gaaaaaaaac gcggaaaccg
    16441 cttcagacaa gccttcgcaa ggaaaattag tcacgactga aagcattggc tgggcgacaa
    16501 aaaaagttct aggattaatc ctaaatttac ttaatgatac aaattagagt gaatttttag
    16561 cccggaaagt tgtctcgttg tgtgagagga tgcgcttacc ggacgcataa taaaccccat
    16621 agcgttacct tcatttgccg catcaacaag ttcagcatgc tcttcttcag tcaaatcatc
    16681 tgtcaaccaa ccgatcacca cactgtaatt gcccgtgcgt aaagcgcgaa ccattgactc
    16741 cactgtgtgg caaggggaga gctggctaat ctgcattact ttcgttaagg gtagcccaga
    16801 tgcctgaacc cattcccgac tcagtttttg ttgcggtgtt aaccagagtt gccagcgcga
    16861 ttgctgaccg agttgctgta acaatggcaa cagtagaagt tgcgtcatca tgggctgatc
    16921 ttcgcgatag acaacttcac tgataagccc ggctgtagtg ttttccgtag agacacgcgc
    16981 aattttactt gctgcggatg agaacgacga agaacgatgt gcatagcctg aagtgtacat
    17041 aatcaatcca gcccctgtga gttactgtat ggatgtgcag tacatccagt gacaacaaag
    17101 atcaacccta ttttcggaaa gagcctcgca aattttgtcg ttggtgacgg gaaaacataa
    17161 attaatcttg ccccttaaga ataagttgcc tattttcgta gttaacggat ccgttaatgt
    17221 gaatcattct tttatgttat gattttaaaa ggaattttat gaaaagcctc tcctataagc
    17281 ggatctataa atcacaagaa tacctggcaa cgttgggcac aattgaatac cgatcattgt
    17341 ttggcagtta cagcctgacc gttgacgaca cggtgtttgc gatggtttct gatggtgagt
    17401 tgtatcttcg ggcttgtgag caaagtgcac agtactgtgt aaaacatccg cctgtctggc
    17461 tgacatataa aaagtgtggc cgatccgtta ccctcaatta ctatcgggtt gatgaaagtc
    17521 tatggcgaaa tcaactgaag ctggtgcgtc tgtcgaaata ttctctcgat gcagcgctga
    17581 aagagaaaag cacgcgcaat acccgggaaa gactgaaaga tttgcccaat atgtcttttc
    17641 atctggaagc gattctcggg gaggtg
//
LOCUS       DAJTUI010000080        17258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTUI010000080 DAJTUI010000000
VERSION     DAJTUI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.080"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(52..531)
                     /gene="xdhC"
                     /locus_tag="OO464_002924"
     CDS             complement(52..531)
                     /gene="xdhC"
                     /locus_tag="OO464_002924"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR6228000.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWARGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="OO464_002925"
     CDS             complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="OO464_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR6228001.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="OO464_002926"
                     /pseudo
     CDS             complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="OO464_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4129..4884
                     /locus_tag="OO464_002927"
     CDS             4129..4884
                     /locus_tag="OO464_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR6228002.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4963..5036
                     /locus_tag="OO464_002928"
     tRNA            4963..5036
                     /locus_tag="OO464_002928"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4995..4997,aa:Gly,seq:ccc)
     gene            5171..6781
                     /locus_tag="OO464_002929"
     CDS             5171..6781
                     /locus_tag="OO464_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6228003.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6813..7420
                     /locus_tag="OO464_002930"
                     /pseudo
     CDS             6813..7420
                     /locus_tag="OO464_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8351..9097
                     /locus_tag="OO464_002931"
     CDS             8351..9097
                     /locus_tag="OO464_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6228004.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
     gene            9395..9886
                     /locus_tag="OO464_002932"
     CDS             9395..9886
                     /locus_tag="OO464_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228005.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            9971..10960
                     /locus_tag="OO464_002933"
     CDS             9971..10960
                     /locus_tag="OO464_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228006.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYVLQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(11646..12224)
                     /locus_tag="OO464_002934"
                     /pseudo
     CDS             complement(11646..12224)
                     /locus_tag="OO464_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(12784..13104)
                     /locus_tag="OO464_002935"
     CDS             complement(12784..13104)
                     /locus_tag="OO464_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCR6228007.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            complement(13303..13608)
                     /locus_tag="OO464_002936"
     CDS             complement(13303..13608)
                     /locus_tag="OO464_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR6228008.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            13716..14426
                     /locus_tag="OO464_002937"
     CDS             13716..14426
                     /locus_tag="OO464_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR6228009.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(14429..14958)
                     /gene="speG"
                     /locus_tag="OO464_002938"
                     /pseudo
     CDS             complement(14429..14958)
                     /gene="speG"
                     /locus_tag="OO464_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(15024..15365)
                     /locus_tag="OO464_002939"
     CDS             complement(15024..15365)
                     /locus_tag="OO464_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR6228010.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            15500..15826
                     /locus_tag="OO464_002940"
     CDS             15500..15826
                     /locus_tag="OO464_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR6228011.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            16032..17246
                     /gene="rspA"
                     /locus_tag="OO464_002941"
     CDS             16032..17246
                     /gene="rspA"
                     /locus_tag="OO464_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR6228012.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
BASE COUNT         4305 a         4220 c         4401 g         4332 t
ORIGIN      
        1 ttaaacacat aacctcagtg aatccggcct gaattcaggc cggatatcct tttacttcgt
       61 tttctcgcaa tccagaactg tatttacaat catctgatac cccgtgcagc gacaaagatt
      121 tcccgccagt ccgcgacgaa tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag
      181 cattgccgtg gtagccataa tcaggccagg cgtacaaaac ccgcactgca ctgtgccgga
      241 tttcgcataa gcctgctgaa catgagaaag ttttccgcct ttcgcttcac cttccagcgt
      301 gcggatctct tttcctctag cccaggcggc aaggtataag caactgtcta ttgctgtgcc
      361 gtcgaccaac accgtacagg caccacattc acccacgcag cacccttgtt tgacacttag
      421 cagtccttgt tcgcggagta attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa
      481 aggcatcccg ttaatggtgc attcggtggt aattgtttcg ctgtgattca ttgcaatttt
      541 ccccccgccg cggcgacggc ttcgctaatc acttttttgg tcatcgtctg gatgagatgc
      601 agacgaaact ctttactggc ccgccatgaa gaacgcgggg cgacatcttg caggacagat
      661 tcgctgatag cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca
      721 tgttggcagc gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa
      781 ttgccgttat ccagtcggca atgtgcggcg cagccaatcg ttgaaatatc cattgcgtcg
      841 cgcatggcat atttaaaatg cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga
      901 aaggcgacga ggatttcgtc atgctcaaga gacactttgc ccggcccggt gtgaaagcca
      961 ttaatcggga cgaaacgaac accgcgcggg gagtggatct ccagtttcgc gtcataaatt
     1021 agcgttggcg tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc accgtaggta
     1081 gcgacgttac ggatctgcgg tccagcaatg aacgtggccg cagcacataa cgccgggaga
     1141 tgacgttgag ttataggatc ttctattagc tgggtaaatg tcgttgcaga gccgatacgt
     1201 agcgagccat cttccgccag cgtaattccc cgcagctccg ccagattatg aatatcaaca
     1261 atatggcgat aacggtcatt gtgatggtgg agctgaatca gtacgtcagt gccaccggcg
     1321 agcagtttgg cctgcgggtt gtcagccagc aggttgatgg catcggcaag ggttgctgcg
     1381 cgatggtaag aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg ccagatggaa
     1441 ctcttcatat aaccgttttg gcgtcagcgg cagtgtattg attgcaacac cggtagccat
     1501 cttcaccgcg ttacgaatag cagcggcaac aggaattatg ggtggctcac ccagtgactt
     1561 atgtccgtat gcggattgcg gctcattgat ttcgacgaac gcgctttcca gttgtggcag
     1621 atccggcatg gtcggcattt tgtaatccag cagattgggg ttacggacca cgccgctttt
     1681 agcatcgatg atcatctctt caaatagcgc ccagccaatg cccattccca ttccgccgtg
     1741 tacctgacct tctgccagca gtggattaag aatatgccct gaatcatgaa cgttgaggat
     1801 gcggttgatg gtgactttgc acagcgcaat atcgaccgtc agatcaacaa aggtacagcc
     1861 aaacgccggt gggttagtgg tggttttgat ggagctttca gcagagagct gcccgccgcg
     1921 ttcagggtgg tagaaagcgt ccatcgccaa atcttttaac gacattaacg gctcttccgg
     1981 tcgttcaacc agcacgatat ggccttttat cagggtcaaa ttcatcgctg actgatgtag
     2041 catgactgcg gcgtgagcga tgattttctc ttttaataat agtgccgcac tgcgcagcgc
     2101 aggcgcggca acatagctct ggcgtgaggc aaatgcgccg ggatcgaacg gcgtaacgtc
     2161 ggtatcttgt gttgaaataa cgcgaacgtc gctgaccgga acccccacgg tttctgccac
     2221 catttgcgag aagacggtgt cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt
     2281 gatggttcca tcctgattca tcagaaggcg cgcgcctgct atttctacgc cgacaggcca
     2341 ggtgttagag gtgtagctaa aacaggcgac gccaacgccg cggcgcaaat tgccttgctg
     2401 gttctggcat tctgcacggc gtttttccca ttcaaagatt ttccggcctt tttcaagaca
     2461 ctccggcaac cctgcgctgc aaatacgttt gcccgtgagc ggattagcat ctccttcgcg
     2521 ggcggcgttg cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg cggcgtcatc
     2581 aagcatagac tcaacggcaa atacgacttg tggcgcgcca taaccacgca tcgcaccagc
     2641 cgaggggagg ttggtatagc aggtctttga actgtaagcg taggcacaac gaggataaag
     2701 gtaagcgact ttattccccc ccgcagaagc gatggagtgc ccgtgagatg cataagcgcc
     2761 ggtgttagac agaacatcca gactataacc tttcaaggtt ccgtcgcggt tcacgcccat
     2821 ttgcccgtca atagtaaaag cgtggcgggt acgggttgcg aggaaacact cttcacggct
     2881 aagggaaact ttcaccggaa tgccgccaag cttgctggtc aggaatgccg ccattggctc
     2941 ttccagtaca tcctgtttat taccaaaacc gccaccgaca aacggtttga tgactcgtac
     3001 gcatgaccag ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg
     3061 ggtgctggaa acgatggtaa ttcgcgagtc atcctccatc cacgccagcg atgttacgct
     3121 ttccatgtga caatgttgaa taacgggagt ctgatagtgc ccctgtacct ggtagtcggc
     3181 ggcatcgatt gtttgttgga cattgcccgt cgacatcgtg ctttgtttca gtaaattgcc
     3241 gccgttatgg attggtgcag cgtcttctgt cagcgccgct tctggcgtgg tgataacggg
     3301 taattcttgc cactcaatgc tgaccaattg cgccgctttt tctgccgtga gttcatcgcg
     3361 ggccacgacg atggcaacgg cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg
     3421 atcggcggta tcgcgcttgt tttcgtcaag tgtctaggca tgcccagctg tagcgaatgg
     3481 aatatcaggc acatcttccc aagtaaaaat cgccagtacg cccggtaaac ttctggcttg
     3541 ttcatcatta atacttacgg cataaccatg tgcgataggg ctacgtacat atttcgcgta
     3601 acacatgccc gccataacat aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat
     3661 ggcatcgacg cgcatgcatg attcacccgt agcggttgct tcccgcgctt ccatagaaat
     3721 cccctcgatt tgaacatttg gttcttatgg caaagattta gtctaagcag gcaagattta
     3781 cgccagaaac gacgataagc gttaggggag gaaggaagtg tgatgacgtt aaaaaaacac
     3841 agatatgata cgaaacagga ctctgaatag ggacagtgat acgtatcaat atgagtggcg
     3901 ataaaatcaa tgtgataaat caggattatt acgcatcagg atgggaaata gggtaaaaag
     3961 tcaggaactt cgatacctct acagcgaata ttttgctgat gatacttccc tttgccaaat
     4021 gaaattaacc tctttctcta ttaccacgtt tttccagaag caagagattg ggtatcaaga
     4081 ggctggctgc tatgatatgg cgtcttgttt tttaagcgag gaaagatttt gagtgcggga
     4141 cgcctgaata aaaaatctct gggtatcgtg atgtttttat cggttggact gctattggcg
     4201 ggctgttcgg gtagcaaatc atccgataca ggaacgtatt ccggctccgt ttacaccgtg
     4261 aaacgggggg atacgctata tcgtatttcg cgcaccacgg gaaccagcgt aaaagagctg
     4321 gcgcgactga acggcatttc ccccccttac accattgaag ttggtcagaa actaaaactg
     4381 ggtggggcga aaagtagcag tagtacacgt aaatcaaccg ccaaatcaac gactaaaacc
     4441 gcatcggtta caccgtcatc agcggtaccg aaatcttcct ggccgccagt agggcaacgt
     4501 tgttggttat ggccaacgac agggaaagtt atcatgccgt attcgacagc agatggcggc
     4561 aataaaggga ttgatatctc agctccacgg ggtacaccta tttacgccgc gggtgcagga
     4621 aaggtggtgt atgtgggcaa ccagctgcgt ggctacggta atctcatcat gattaaacac
     4681 agtgaagatt acattacggc ttacgcccat aatgacacga tgctggtaaa taatgggcaa
     4741 agcgtgaagg ctgggcaaaa aatcgccact atggggagca cggatgcggc atctgttcgc
     4801 ctgcatttcc agattcgtta ccgtgcaacg gcaattgatc cgctacgtta cttgccgccg
     4861 cagggcagca agccaaaatg ctgatggcga attaatcagc agtcagcagc gcggcacttg
     4921 ctaaggagag cgtaaggttt ataatgcctt acgcatctcg aagcgggcgt agttcaatgg
     4981 tagaacgaga gcttcccaag ctctatacga gggttcgatt cccttcgccc gctccatgac
     5041 acttcttaaa gtctactaac ttactgaatc ctaagggata cggcttaacg ccttccgata
     5101 gttttactca ctgtaaccaa cactgtgact aactttgttg aagtagtgag cagacctaag
     5161 gtctcagtac atgctcaagt caagaaccta cctttatcag cgtaatggtg tcttttacat
     5221 ccgtcttagg atgaagacca ctagtcgcct gaccgcttcg cttccttctc acaaccgtta
     5281 taaactagcg tcagtatctt tacggaccaa ggatagacgt accgctatgg ctcactcacg
     5341 gcacatcaag tcagcactta aagcaatcca tgcagacaac cctaacgcct cttatgagga
     5401 gctacgggag cacctgaaga ccattgtcga gtgggaactt agtgtcagcc gtgatgacct
     5461 gaacgacccg gagtcctacc agctttacgt tgaccagtac gatgacatta agtccaacct
     5521 tcgggaagct gtagcgaccg agcgtctgac tgtagaccag catcgctata ttaatgacgt
     5581 tatcggtgtg cttaaggcct gtcaggatag actcaatggc gatagctctg gcctattgtc
     5641 ttaccttgag cctgagactg gcagtctaag accgtccgtc tccctatctg tattggcgga
     5701 acctgaagtc cctgaaccta aagcactgac cctcgcgtct ctcattgagc agtacgagca
     5761 ggagaacgcc cagaactgga agccagcgac cctaagcgaa aaccgagcgt cacactctac
     5821 gctaatcgag atattcgact atctggatat tcaggatgtc ggcaaggcga cccgtgcaga
     5881 tatgctcaga gttcgtgaag tcctccagca gctaccgaag aaccgcaagc aacgctttaa
     5941 gtctatgccg ctgtctgact tgctgaaccg ggagtctaag actgactgtc tggacgtcgt
     6001 taccattaac aataaatatc ttatcaagat ggctgctgtg tttaagtggg cggtacgcaa
     6061 cgacttgata gctaagaacc tgaccgaagg tcttgagctg aaagtccctc agcgtaaagc
     6121 ctccgacgct cgtgatgcgt tcagtcctga gcaggtaggg caactactgg tcgcagctaa
     6181 agcgtactcg cagaagacct caggtaagcc ataccattat tacgtcaccg ctctggctgc
     6241 gattacaggt gcgagactta acgaggtggc ccaacttcag gttaaagatg tcaggaccac
     6301 tgaggctggg accgttttta tccatatcaa cgaagacgat agcagcctgc cgggtaagag
     6361 tatcaagaac gcccacagtg accgctgtgt cccgttggta gatggtgctt atggttttgt
     6421 attggctgac tttatgtcac tcgtggaaga ccgtagaaag actgagggtg ataatgctat
     6481 ggtgttcaat ggcctcaagc taatgaagaa cggctacggt gagcaggtta gcaagtggtt
     6541 taaccgaacg ttgctgccca aagtcctcgc tgaccgtagc ggcttagctt ttcactcgtt
     6601 tagacataca gttgcgaccc agttaaaaca acatggggtc gagttagcgt atgctcaggc
     6661 gattatgggt catagctcag ggtctataac ttacgaccgt tacgcgaaag aggtcgaggt
     6721 ggaaacgctg aaagaaaaat tagcggagtc attgtcagta aaaaaaatag acggaaagta
     6781 atcgcatagg gatagcggaa tgtgaggacg taatggtcaa agaattttta gttagcaaag
     6841 aaaataagcc ttcatatgaa tgtatgaata ctgctgaaac ttgtttgggg ctggctggaa
     6901 tggcttcgga agattggcaa cgtgagcatc attgccgcac aggtataata tttactgctt
     6961 ttgctattga ggcaatgttc atcttctata gaaaacaggt tgaccccggc tacgataaga
     7021 cgcagaaaga gtgcagaaag acaatgcaca agaatacgtt aaaattgtgc ggtataaaca
     7081 attatatggg gacaaaacct taccaaatta tcaaagagtg tcttgaggta agagacgcta
     7141 ttgctcacgg cgattcgtat acttcgagct ttaatttctc ggcggatcat ttggataacc
     7201 aagacgatat tgcaaaagtg tcctggcggt aaactctgaa caatttcggg gattaactgt
     7261 tgagcgcctt gcaaaataca ttgaaatggc aaagcatatt gaccatgaga tttctgataa
     7321 tggttacagg ccttctgaag gttaccttcc agcagccgag cgcagaagtc tgatgttagc
     7381 ttttggcgtc agtggtgtgt caatgtggcc cagtgcttga gcgataatac acctcactat
     7441 agaggaatgg acccttaact atcactatag gggagagacc ctaagtcccc accttaatag
     7501 cagtcacgac ccatacacac attcattaag tctcaggctt ccggtagcgg accgattgga
     7561 aaccatccgc agacgcctag tgttaaatca ccttggcctg agaccattcc ttttaacgta
     7621 agctatcttc ccttttgaca ggcggggagc agtagaccac atccgacttt aagttggctc
     7681 tgtgataatc gctgcgcgga taactgaaga accaagacac cattactgag gtctgccatc
     7741 catttgtgga caggcggaaa ctcaaagagg ttagatcagt ggtgtctgag gttggagctt
     7801 tgagtcttga ccagcaggct ggaggagacc atgagtttat cttgttatgg attgtgttat
     7861 cacaattcca ataagaagaa ctttaagtcc agcctccact cccgccaacc cgaagtaaaa
     7921 ttatggtcgc tatgatgtgg tctccatgct tcgggtctta ctggaagtac tgaacacttt
     7981 cgcgtcaagt tctccgaagt tcattcctgt ttgactaaac tatcactata gggaccatat
     8041 ctcgcctctg acctcctgag agacggcaag cgagaaaccg tggtccacgc tagcaagcta
     8101 gccaccagcc taaggcaaga cagcctctgg tcagcctgtg tgttctttgg tctcgtgact
     8161 taagtctatc accacacacg ctcggacatg ctgtcgaacc ttcatctcta tggtcatcgc
     8221 agaccactaa ccccaattac ctcaagtcat tctgtttgcg tcctgttgcc tgctggtctc
     8281 cctttacgtg ggggcttgag taggtcagtg gggtgcgact tcagggtgca aacaaaagga
     8341 gtacacgtaa atgtcccaac aagaaatcag catcattaac ctcgaccagc ttgtgtccat
     8401 gacctcagtt gaaattgctg agctgacagg aaaagaacat aaacacgttt tacgagatat
     8461 tcgtaatatg gtcgaagagc ttaacggggc caaaactgag cactgctcaa ctttgagcag
     8521 cgagcttaac gggtccaaat ttgggctcgt tggcgaggaa gtatacaagg acgcgaaagg
     8581 tgagagccga acaatgtatc gtctcgaccg taagcatact ttcattctgg tcgctggcta
     8641 ttccgttcac ctccgcgcca agtgttacga ccatattcag acgttagagc gtcgagtcct
     8701 tcaactggaa gaccagaaga agcgagcagc cattcagtcc gctaaccgcc gaggcgtgac
     8761 ttggggcgac tactgcaaaa cctatggtct gcccgctcag aagctaatga ccgcacttct
     8821 ccagcatcgt ggcctctttc gtaagaaccc aatcagtaac gagtggtctg taaatccgaa
     8881 gtattccgac tgctttcgta tcattaagcc gtcagaccag aagttctcag caggtgggta
     8941 taacttccgg ttcaacgcta aggggctgga ggtcttcggt aagcctgaga tggttgacaa
     9001 gatgcgaggc atccttattg cattcactgg caccgaccag cagaagcaag agcacctcct
     9061 gaagctggca caatccggta aggtggaggg aatctaagat gatgattgac ccgcgcacac
     9121 ctgaggggcg catgactctc cgttaccgtg gctaccggac tgaagtcctc ctcagagagc
     9181 ttggtctaga cccggaagat gagacccgcc agcaccaatc ccgagacgag ctaatagccc
     9241 aactggtcgc tatgaaactg ccgcttaacc gttagtagct cacctactca atacctaatt
     9301 taaacacctt ttgagatgac cgatgcgggg agacccgtag gccttactgt gcctgcgata
     9361 ccgatagtct caactctcaa gaggagaatt acctatgatg ttcaacaaca acaactggaa
     9421 gctgtcagtc accgacatca acctttatga aaacactgtg agtctggacg gtcagcccta
     9481 tccgctgtcg ctcgctatca agaccctcat tccgggctac ctgtcaggcc tgccgtctac
     9541 cagccgcgag gcaatggagt tactggaagc tctggctgaa gctggcgtga ccattggcaa
     9601 cttcttcagt aatgacttga tgaccgccta tggtcgccgc cagatgaaca aacgagctga
     9661 agctgagcgt atcgctaaag agcaacgact tcaggctgag cgtatgcgtg aagagaacat
     9721 gaccgatgca gagtggcaga aagagctaca gcgccgcgaa caggtcaaag ctgaacgccg
     9781 gacctatggt gagagcctcc gcagtgctac ccattccgct ggtcgctccc gtgccgctat
     9841 tgtcgctgac cttgagtcag gcggtaactg gatggacagc ttgtaaaaca cacagtccct
     9901 gacccgatgt tagggactcc tcagttttga ggactcacag tatttaaact aatttaggag
     9961 attgattatt atgactacga ttaaagacgc tttccagttt ggcattgagc cagtacgcat
    10021 caccgatacc gacaacattc aggtcaatga aggtctgccg actaacgctg acccgcaggt
    10081 ctatgtcctc cagttagcta agacggttaa ggctatgctg aacggtgtac ttaaagacgc
    10141 tcaagagaac atcccgttcc ctgtagaagt cctgccgacc cgtaacagct taccgacccc
    10201 aatcattgct cacactctgg ctgaccgctc tgtggtcgtt cctgtccgtg gtggcaagcg
    10261 acctgaagtc gtgaccgttc cgtctggaca agaaatcgtg gtcgaaccaa tcgagcaggc
    10321 tattctcatc tctgagcaga ccaagctctg ggacgccaag tcatccaccg gattcactca
    10381 gggcacactt cagcaggacg ctatgaacat ctgtgagaat gtggtccgta ccatcaacgc
    10441 tcgcatggtc gacgttctgg aatcctctaa gctgctgaag accgtagagc ttcctgtcct
    10501 gactggtagc ctgaccgcta aggcagacgc tatcatggac gctctgtatg agaacacaga
    10561 gtcttctttc ggttctgagg tgtctgacta cggtattatc gctcatgaga gccaactgaa
    10621 agcactgtcc cgtctggctg ctaagcaagg cttctccggt gaagacgcta tcgtcgatat
    10681 gctggggaca gacatcgcct attacaacgg tgaagacaag ggtgtattca tgctggctaa
    10741 acgcttcacg gccctgagct tcggctgctt ccgtcacgac ggtgagaata ttacggtcgt
    10801 cctttcacgt gatggcgact ctcagtccca cgacttggaa atcctcggta aagtgttcgt
    10861 tgtcgctgaa gctgctacga ccatcaagat gggcactggc tcagctactg ccgtcctgcc
    10921 tgtagtcaaa cgcctgaagt tcaccaagac cgaagcgtaa gaccaatggg taagagcatt
    10981 agtaaaggct tcaggtccat cgccaagggc ctgacaggtg gtggtgggac tcaaggtggg
    11041 gcttcggtcc cgcctcctgt agctcctcct gtagctcctc cagatgacgc tcagcaagct
    11101 gtagtcgata cccaaggtca ggagaccgag ggaggtaaga ccatgagcag aggtaagtct
    11161 ggactgaata tcagcaggac ttcaggtggt ggcttgtcac tgtgattaac taactacggt
    11221 ctggaccaat ggtctgggcc tttgttttgt actagttgtc tagtgcggta gtgcaataga
    11281 agataggggg gggctttatg tccatgactt ccgaagacct aaggtccctc ctgactctgg
    11341 tctataaact cgtgttcctc tctgtgggcc tctatatggt cctctccggt cgactcggtg
    11401 tgaatgtatt cgacactctg tctaaggctg tgggtgggct tctgggggct taggtttgta
    11461 ctcacagtgg gaccgatggt ttctgacaga aaaatgtgaa tgaccatcta atacattaag
    11521 ccagcagact tcccccatag cccgcctcaa gtccagaccg aggccaccgg agggtcctga
    11581 ccagccgtct agacatcccg tctaatgaca caagtctggc acaaagaatg acacaaggtc
    11641 ttgactcaat gcaggacctc aggcctacta agggtccgct tcaagtcctt gtagttccag
    11701 atgaacttaa tgggcacatc cagtttatct ttgccccgga caccgtcgcg gagtgccgtc
    11761 atttgcgggc catgatcgat agcatccgtc cagctgaagc aggagattga cgttttgacc
    11821 ggattatcca gcaccggtag gcgttctatc gtaatggtat aggtcgattc acgcgcgcca
    11881 ctatttccgc cgctgatgcc gacattgccc gtcaaaatag gtaacatagc aatagcgcgt
    11941 gcagtcagct cgccgtttgc ctggcgttgc ggcccccagc cctagcagat ataagcgggt
    12001 tttgttgtgc caatttcacg cgccagtttg atgatacggt ctaccgggat accggtaatt
    12061 tgcgaagccc actgcggcgt ttttgctgtt ttatcgtcac cttcaccaag aatataggct
    12121 ttatagtgac catttttggg tgcatctgcg ggtaaggttt tttcgtcata gccgacgcag
    12181 tatttatcga gaaaaggttg atcaacgaga ttttcgttaa tcaataccca ggcaataccc
    12241 gcaaccagcg cggcatcggt gcccgggcga atagggagcc attcctcttc acgaccggca
    12301 gccgtatcgg tatatcgcgg atcgataaca atcattttgg cgttcgattt ctcgcgcgct
    12361 ttttcaagaa gataagtgat gccaccaccg ctcatgcggg tttctgccgg gttgttacca
    12421 aacatcacga ccagcttgct gttttcaata tccgtggtgc tgttgccatc attactgccg
    12481 taggtgtagg gcatggcaca ggaaatttgc gcggtgctgt aggagccata ctgattgagt
    12541 gaaccgccgt agcagttcat caggcgtttg accgccgagg ctgatggcga agagcgggtc
    12601 atattgccgc caacaatccc cgaagagtac tgaatatata cagcttcatt gccatattgt
    12661 tcgacggttt ttttcaggct actggcgata gtatccaggg cttcatccca gctaatccgt
    12721 tcgaatttgc cttcaccgcg tttacccacg cgtttcattg ggtcattcaa gcgatcggga
    12781 tgattaatac gccggcggat ggagcgaccg cgcaaacagg cgcgtaccct gatggttgcc
    12841 gtactcatcg ctgccggtat tgtcagtttc cacccaggtc acttcattat ctttaacatg
    12901 tagacgaagt gcacagcggc taccacagtt gacggaacag gaaccccaga tcactttttc
    12961 gctggcctgt tgtaccgctg ccgctgcact gcgcagggtg aacggtaaag aaaaaccgcc
    13021 tgcagccagc gccagagaac ctgtcgcggt tgatttaacg agtgttctgc ggctgatgcc
    13081 caccattcgg tcatttttgg acataactca ctccctgttc tttattgtta tataaatgtt
    13141 tatacattga atatttagcg cgctaacaat agagggagtc tacccatttt gggttaagaa
    13201 ttattaatcc acatcaatag aagggtatga gtaataaggt gagattatgt tgtatgttca
    13261 aatcgccgga tgtgtcatat ccggcgttca gtcgataatg tgttactgcg gttcggcagg
    13321 cgcgccatcc tgggtagact gcgcgggagc agagacgtta ccgctggtgg tgcggttgta
    13381 gagaatttta tgcgtatcat tagcgcaatg cccgacgacc tgggaatcag gctgatcaac
    13441 ctggtcattg ggtacaatac ttaacgtgaa gctgctttcg ggtacgccat tattgataat
    13501 gcgctgtgat atatcgctct gtatgcgctc acaagatccc ggcgcggcga gtaccgcggg
    13561 tgaggcgagg gcgagcagaa gcgcggcaca gcaggttgag agtttcatca taagctcctt
    13621 acgcgaagat aacttcttta agcatagcat ttaacgtgta aagtactgta tttgctacta
    13681 tgattgagaa tcatctctac tctctggtga ctgttgtgaa atacaaatta ctaccatgct
    13741 tactcgcgat attcctcaca ggatgtgacc gcacagaggt aacactttca tttacccctg
    13801 agatggccag tttctctaat gaattcgatt ttgatccgct gcgtggtccg gtaaaagatt
    13861 tcactcagac attaatggat gagcaaggtg aagtgacgaa acgtgtttct gggactttgt
    13921 cggaagaagg ctgttttgat tcactcgaat tactggatct ggaaaataat accgtggtcg
    13981 ctctggtact ggacgccaat tattaccgtg atgccgagac gctggagaag agagtacgtt
    14041 tacagggaaa atgccagcta gcagaattac cttctgccgg ggtgagttgg gaaaccgatg
    14101 ataatggctt cgtgattaaa gccagcagca aacaaatgca gatggaatat cgctatgatg
    14161 atcaggggta tccgctgggt aaaaccacga aaagtaacga caaaacatta tctgtcagcg
    14221 ccacgccatc aacggatccg atcaaaaaat tagattacac agcggttact ttactaaata
    14281 atcaacgagt tggtaatgta aaacagagct gtgaatatga cagtcacgcc aatccagtgg
    14341 actgtcagct gatcattgtt gatgaaggag taaaacccgc cgtcgaacgg gtttacacca
    14401 tcaaaaatac gatcgattat tattaatgct attgtgcggt cggcttcagg agagtctgac
    14461 ccggtgtttt gtgctctgcc agatactgat gctggaatat acacatgcga atggcattac
    14521 gatattgacc attaataaaa aactcatgca tcaattcacc ttcaaccgta aagccaagct
    14581 tgcggtaaat gtgaatcgct ttttcattct ctttatcgac gatcagatac agcttataga
    14641 gattgagaac ggtgaagcca tagtccattg ctaatttggc ggcacgggtt gccagacctt
    14701 tcccctgata ctccggggag ataattatct gaaattctgc ccggcgatga acatggttaa
    14761 tttccaccag ctccaccaga ccggcttttt cgccgtcaca ttccaccaca aagcgccgtt
    14821 cgctctgatc gtgaatatgc gcttatcata cagatcagag agttcaacaa aggcttcgta
    14881 gggttcctca aaccagtaac gcatcacact ggcgttattg tcgagttgat gtacatagcg
    14941 taaatcttca cgctccagcg ggcgtagctt aactgtgggc gcttggcata acgtgtcctt
    15001 acattcctta aatcaataac aggttagggg gtaataacgc ggccagttcg acggtccagg
    15061 cagcgcaaag tattgggctc ccagtaggca ttgatgttgg cgctttgctc acatttatcg
    15121 cggttatcaa aagcggcgtc ggctttatcc cactcttttt cagtgcgttt attcactttc
    15181 tggcgcagat tgcgcgtgtc attccattgc tctttttcca tagcggcgtg ctggcggctt
    15241 tgtgcactgt cgccagactc aatcaccagt ttgttagttt cggcatgaac agttgtgctc
    15301 aatgccagtg cgcaaggcag cagaaaagcg agcaggccga ttcgtttgct gagagtgatt
    15361 ttcataattc attccctgta tgaatgatta aaggtgattc tacaccatcc actgcgaacg
    15421 caaaacgtac caggaggatg tttatattga tgatattatg tcgccctata actatacatg
    15481 atgtcaataa gtgacaaaga tgattaaaac aacgttacta ttttttgcta ctgcgctgtg
    15541 tgaaattatt ggatgctttc tgccctggtt gtggttaaaa cgaaacgcca gtatctggct
    15601 gttgcttccg gcggggattt cactggcgct gtttgtctgg ttgttaacgt tgcatcctgc
    15661 ggcgagtggg cgtgtttacg cggcttatgg tggcgtttat gtctgcacgg cattgatgtg
    15721 gctgcgcgtt gtggatggcg tgaaactaag tctttatgac tggacgggtg cgttgattgc
    15781 gctttgcgga atgttgatca ttgttgcggg ctgggggcgc acgtaggaac ataaatccat
    15841 tttatcaata agatacgagg aagtgtcagc tgacaaaagg tattctattt catcttttgt
    15901 caaccattca cagcgcaaat atacgccttt ttttgtgatc actccggctt ttttcgatct
    15961 tcatacttgt atggcagtag ctcagttgcg tagatttcat gcatcacgac aagcgatgca
    16021 aggaatcgaa catgaagatc gtaaaggctg aagtttttgt tacctgtccg gggcgtaatt
    16081 tcgtcacatt aaaaatcacc actgaggacg gtattacggg ccttggggat gccaccctca
    16141 atggacgtga gctttccgtg gcctcttatt tgcaggatca tctttgtccg cggcttattg
    16201 gtcgcgatgc gcaccgtatc gaagatatct ggcagttttt ctataaaggt gcttactggc
    16261 gtcgcggtcc ggttacgatg tcggccattt cagcggttga tatggcgctg tgggatatta
    16321 aagccaaagc tgccaacatg ccgctttacc agttactcgg cggcgcgtct cgtgaagggg
    16381 tgatggttta ttgccatacc accggtcaca gtattgatga agctctggat gattatgccc
    16441 gtcatcagga gctgggattc aaagccatcc gcgtgcagtg cggaatccct ggtatgaaaa
    16501 ccacctacgg catgtcgaaa ggtaaaggtc tggcttatga acccgcaacc aaaggacagt
    16561 ggccggaaga gcagctgtgg tcgacggaga aatacctcga tttcatgccg aaattgtttg
    16621 acgcggtacg taacaagttt ggttttaatg aacatttgct gcatgacatg caccatcgct
    16681 taacgcctat tgaagcggcg cgctttggta aaagcattga agattatcgc atgttctgga
    16741 tggaagaccc gacgccagcg gaaaaccaag aatgcttccg tctcattcgc caacataccg
    16801 tcacacccat cgcagtgggt gaagtcttca acagcatctg ggactgcaaa caactgattg
    16861 aagagcaact catcgattat atccgcacca cgctgaccca tgcaggcgga attaccggta
    16921 tgcgccggat tgccgatttt gcttcgctgt atcaggtacg tactggctca cacggtcctt
    16981 ccgatttgtc accagtctgc atggctgcgg cgctgcactt tgatctgtgg gtccccaatt
    17041 tcggtgtcca ggaatacatg ggttattccg aacaaatgct cgaagtcttc ccgcacaact
    17101 ggactttcga taacggctat atgcatccgg gagacaaacc gggtcttggt atcgaattcg
    17161 atgaaaagct ggcggcgaaa tatccctatg aacctgctta tctaccagtc gcacgtctgg
    17221 aagatggcac gctgtggaac aggtaaggag taagataa
//
LOCUS       DAJTUI010000081        17243 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTUI010000081 DAJTUI010000000
VERSION     DAJTUI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.081"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..267)
                     /locus_tag="OO464_002942"
     CDS             complement(<1..267)
                     /locus_tag="OO464_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR6228013.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGAT"
     gene            complement(275..625)
                     /gene="chbA"
                     /locus_tag="OO464_002943"
     CDS             complement(275..625)
                     /gene="chbA"
                     /locus_tag="OO464_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR6228014.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(684..2032)
                     /gene="chbC"
                     /locus_tag="OO464_002944"
                     /pseudo
     CDS             complement(684..2032)
                     /gene="chbC"
                     /locus_tag="OO464_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2117..2437)
                     /gene="chbB"
                     /locus_tag="OO464_002945"
     CDS             complement(2117..2437)
                     /gene="chbB"
                     /locus_tag="OO464_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR6228015.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(2736..3074)
                     /gene="osmE"
                     /locus_tag="OO464_002946"
     CDS             complement(2736..3074)
                     /gene="osmE"
                     /locus_tag="OO464_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6228016.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3276..4103
                     /gene="nadE"
                     /locus_tag="OO464_002947"
     CDS             3276..4103
                     /gene="nadE"
                     /locus_tag="OO464_002947"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6228017.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4333..5220
                     /gene="cho"
                     /locus_tag="OO464_002948"
     CDS             4333..5220
                     /gene="cho"
                     /locus_tag="OO464_002948"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6228018.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5180..5755)
                     /gene="ves"
                     /locus_tag="OO464_002949"
     CDS             complement(5180..5755)
                     /gene="ves"
                     /locus_tag="OO464_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6228019.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(5958..6443)
                     /gene="spy"
                     /locus_tag="OO464_002950"
     CDS             complement(5958..6443)
                     /gene="spy"
                     /locus_tag="OO464_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR6228020.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(6773..7741)
                     /gene="astE"
                     /locus_tag="OO464_002951"
     CDS             complement(6773..7741)
                     /gene="astE"
                     /locus_tag="OO464_002951"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR6228021.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(7734..9067)
                     /gene="astB"
                     /locus_tag="OO464_002952"
                     /pseudo
     CDS             complement(7734..9067)
                     /gene="astB"
                     /locus_tag="OO464_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9064..10542)
                     /gene="astD"
                     /locus_tag="OO464_002953"
     CDS             complement(9064..10542)
                     /gene="astD"
                     /locus_tag="OO464_002953"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR6228022.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10539..11573)
                     /gene="astA"
                     /locus_tag="OO464_002954"
     CDS             complement(10539..11573)
                     /gene="astA"
                     /locus_tag="OO464_002954"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR6228023.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11570..12790)
                     /gene="astC"
                     /locus_tag="OO464_002955"
                     /pseudo
     CDS             complement(11570..12790)
                     /gene="astC"
                     /locus_tag="OO464_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13236..14042
                     /gene="xthA"
                     /locus_tag="OO464_002956"
     CDS             13236..14042
                     /gene="xthA"
                     /locus_tag="OO464_002956"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6228024.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14209..14922
                     /locus_tag="OO464_002957"
     CDS             14209..14922
                     /locus_tag="OO464_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6228025.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLLYARHK
                     HVDLSASRRSPLTHPKNEG"
     gene            14927..15604
                     /gene="ydjY"
                     /locus_tag="OO464_002958"
     CDS             14927..15604
                     /gene="ydjY"
                     /locus_tag="OO464_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR6228026.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            15619..16326
                     /locus_tag="OO464_002959"
     CDS             15619..16326
                     /locus_tag="OO464_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6228027.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16326..16874
                     /locus_tag="OO464_002960"
     CDS             16326..16874
                     /locus_tag="OO464_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6228028.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            16884..17210
                     /locus_tag="OO464_002961"
                     /pseudo
     CDS             16884..17210
                     /locus_tag="OO464_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4248 a         4589 c         4237 g         4169 t
ORIGIN      
        1 cgtggcacca aactcataaa aactttgatg gtgcgaacct aacggaataa aaacaaaatc
       61 gccccgttcc agtaacacgc gcttaccgtt aatctcctgg aaataacgcc cggttaatac
      121 cagagtaaat tcataatagt cgtgctgatg cagtccgctg atactctcag ttttgttata
      181 gataaacaca tggaagtttt tgccattaaa caactgctgt tctcgggcag tggcaatttc
      241 cggcgcgtta atcactggct gcatcatcga ctccttatgc cttcagtttt tcatgaagct
      301 caattaattc agtaatcagt tcacgcgcaa gcatggacgt cattaaatga tcctgagcgt
      361 ggaccagcac cagactcact ttcatcttac cttcgcccgc atcgccttca atcagtttcg
      421 tctgtaccag atgcgcttca ttcaatgcca tccgtgactg atccatcatg gctttcgctg
      481 cggcaaaatc gccctgcttc gcctgtttca atgcggcata cgccaggcta cgcgcttgtc
      541 cagagttgat gataagcccc atcaccactt cttccagctc ttcagcttct gtttgcgtat
      601 caggaatatt atcgagatcc atcatacatc gttcctctct cttcttaccg gcaagattac
      661 ccgtaccggc atcgattaaa atttcagggg ttagcgatat cttcttcgct ctcttcttta
      721 tcaatcgcat tttgtgcttt gttagccacc acaacaaagg gcagataaat taacgttgcg
      781 atgccaaggt tgaagagtgc gaccagcaat gcggcgacgc taccgttggt gttaaagaag
      841 gctcccagac cggttggcat ggtccacggt gcaatattgg tcaccggagg aatgatgccc
      901 atgtagtacg ctgcgagggt gattgccgcc agaatcggtt gtaccagtac aaacgggata
      961 aacatcaccg ggttcatgat aattggcaga ccaaacagaa tcggttcgtt aatctggaag
     1021 atgccggacg gcagcgccag ttttgccacc tgacgataat cagcacggcg agaggcgata
     1081 aagatagcca ggatcaggcc taaagtcgca ccactgcccc caaggaaaat aaaggagtcc
     1141 agcatcggct tggcccagat atggaaggtc ttaccggctg ccagcgccgc ttcgacggaa
     1201 ccatattgct gataggtcgc gatattttcc agcgcccacg gcgtcataat gccgttgtcc
     1261 agtgcggtca gcgccagcgc gccatgaata ccgaagaacc agagcagtgg aacaaagatc
     1321 acataggccc agcccaccac gctacccaac gatgccagtg gggttgagat ggtatccata
     1381 atgatctgat ggaagttggt gccccaggta ttcaacgccc aggcaataat ccccatcacg
     1441 gaaagaataa taaagccggg aattaatgcc gagaaagaac gcgatactga agcaggtacg
     1501 ctgtcgggca gtttaatgac ccaattgcgg cggacaataa aggtaaacat ttctgccacc
     1561 accaggccaa taataatccc ggagatgata ttcgccccac ctaaccagtt tgcgccaacc
     1621 gcataggcct caccgacact atatggggtg acggtcataa atgctgcaac ggataacaac
     1681 ccagccgcca gcgcatcgac tttacgctct tctgccagcg ccatgccaat aaagaacggt
     1741 gccattaaag acattattcc taatgttccg ttatatacgt tgccgccaat acctttcaga
     1801 ccattaagtg tttcaatggt tgaggcgtcg aggcgaatac ctaaggaata aaaaacgacc
     1861 cctccccaaa gcttagaaaa acgttgttaa ttaatacaaa catggccccc gcaagggtta
     1921 acggcattaa gcgaataaag ccatttttga ttgcattaac gtgtggctgc tttcctattt
     1981 taactgcaaa agggaggagt accttttcaa gcgatgcaat aacattactc atagaaaaat
     2041 acccttaaaa accgcaattt aaatattacg gtattgattt atgaaataac tctttgacgg
     2101 gaaaatttaa aataaattaa tttgctgcgg cttttttaat cgctgcaacc gcagccttaa
     2161 gcacgcctaa accatcgact ttgccataaa gcagcgagtc aattacttca accggtttgt
     2221 tgggtaacaa acgctggatt tcgggcaaca tataagcaat ctgcggccct aataacacga
     2281 catcggcatt ctgacctttt tcaccagcca gtgtttccgg aaatgcttca ataatgaccg
     2341 gaacttcata tttttctgcc tgtgcgcgca tttttgatac cagtaaagag atagacatgc
     2401 ccgcagaaca aaacagataa atgtgtttct tttccataaa actgccctca tcgacgatta
     2461 tctgtcagcc agacactccg caagccttga cctgcttcca tgctctgggt aacttgcgaa
     2521 accaacatga ctttttttgt ggctgaaagg agtatacggt atagacctga gcaatagtat
     2581 ttctttgacc ttataaattg tttctccttg acctgtggtt atgaccctct tcacatttcg
     2641 ggcaaataat tcgcgaagat aattaagaac tcaggccaaa aaaaaccggc gcaatggccg
     2701 gtttccgttg ttactcaagc tttcagacga attgattact tcgcagcctg tggatcagtg
     2761 tcgtattcag cacaggtctg ataaccggag ttgatgacat gtccggtatc atctaacgcg
     2821 acaaagtagg tttctgcttt accatcacgt tgacccagga tgtaggtctg gcaagtaccg
     2881 cgagcatgga tcatgctcac ttcagacgaa ggtttacccg caatttgtgc aacctgcgcc
     2941 cggctcatgc cttttttgac gtctttcacc acaggctgta caaactggtc tttggtacga
     3001 tcataagctg tacaacccgc cagcatggtt aataccgccg ctgcactcag aattcctgcc
     3061 atattcttgt tcatattccg tcctcttgtt tatcagcgtg ttagataagc ctggaataca
     3121 ttgggcgctt tttcaagccc gtgaacgaaa cggctccgct ttcagaggat tcctgtatga
     3181 cgttttaacc accattcagc ccgctgtcgc ttgtcgtttc agtagcaacg ggttagcttt
     3241 aaggaagttt tgtcttttct gtctggaggg gttcaatgac attgcaacaa caaataataa
     3301 aggcgctggg cgcaaaaccg cagattaatg ctgaagagga aattcgtcgt agtgtcgatt
     3361 ttctgaaaag ctacctgcaa acttatccgt tcattaaatc actggtgctc gggatcagcg
     3421 gcggtcagga ctccacgctt gccggaaagc tgtgccagat ggcgattaat gagctgcgcc
     3481 aggaaactcg caacgaatca ctgcaattta ttgccgtacg cctgccctat ggtgttcagg
     3541 ctgacgaaca agattgccag gatgccattg cctttattca accggatcgc gtattaaccg
     3601 ttaatatcaa gggcgcggta ttggctagcg agcaggcatt gcgggaagca ggcattgaac
     3661 tgagcgattt tgtccgtggc aatgaaaaag cgcgtgagcg gatgaaagca caatatagca
     3721 ttgcgggtat gaccagcggt gtcgtggtgg gcaccgatca tgcagcagaa gccattaccg
     3781 gattcttcac taaatatggt gacggcggta cggatattaa tccgctgtat cgtctcaaca
     3841 aacgccaggg taaacagtta ctgacggcat taggttgccc ggaacacctt tataagaaag
     3901 ccccaactgc cgatcttgaa gatgatcgcc cttctctgcc agatgaagtg gcactcggcg
     3961 tgacctatga caatatcgac gactatctgg aagggaaaaa cctacctgaa caggtcgcca
     4021 gaacaataga gaactggtat ctgaaaaccg aacataaacg ccgtccgcca attaacgttt
     4081 tcgatgattt ctggaaaaag taataatttg cacctattgg attgtgcgaa aaagagtaat
     4141 ttgttcacgc cggatgcggc gtgaacgcct tattctacct acaaaactat gcaaattcaa
     4201 tacattgcag gagtcgaata ggcctgacag gcgtagcacg tcagacggtg taacctttgt
     4261 catcaacccg cctctttttt aatcgcctcc cgcctgttac actggataga taaccagcat
     4321 tcggagtcaa cagtggtacg gcgtttaact tctccgcggc tcgaatttga agctgcggca
     4381 atttatgaat atcccgaaca tttacattca ttccttaatg acttacccac ccgaccaggg
     4441 gtgtatctgt ttcatggcga aagtgacacc atgccgctct atatcggcaa aagcgttaat
     4501 atccgcagtc gggtgctttc tcatttacgc accccggatg aagccgccat gctacggcaa
     4561 tcccggcgaa ttagctggat ctgcaccgca ggcgaaatcg gcgctctgct ccttgaagcg
     4621 cgattaatca aagaacaaca gccgctgttt aataaacggt tgcgccgcaa tcgccagctc
     4681 tgtgccctgc aattaaatga aaagcgcgtc gatgtggtgt atgccaaaga ggtggatttt
     4741 tcacgagccc ccaacctgtt tggcctgttt gccaataggc gcgcagcttt gcaagcattg
     4801 cagagcatcg ctgatgaaca aaaactttgt tatggcctgt tgggactgga accgttaagt
     4861 cgcggtcgtg catgttttcg ttcagcgcta aaacgttgcg ccggagcatg ctgcggtaaa
     4921 gagagccatg aggaacatgc gctacgcttg cgccagtctc tggagcgttt gcgagtggtg
     4981 tgttggcctt ggcaaggtgc ggtggcactg aaagaacagc acccggaaat gactcaatat
     5041 catattattc aaaactggct gtggctgggg gcggttaatt cgctggaaga agcgacaacg
     5101 ttaattcgga cacccgccgg gtttgatcac gacggttata aaattctttg taagccgctg
     5161 ctttccggta actatgaaat tactgaactt gatccggcga atgaccagcg agccagttga
     5221 tttcactgaa caacagttta ccttgcggtt gtaaaagccg cagggtgtgc ctgccatcga
     5281 accagcacac gccctggtcg gtagtgagca gtttgtcgcc cagttgccac gccccgttaa
     5341 tcacaaacac caccccgccg cgtgagccaa aggtagtaaa cgtacgttcg gcaattctga
     5401 ctttcgcctt acagacatcc aggcgcgtca taatattgaa atccatcgac atctgccctg
     5461 ccgtcagttt cgcttttacc acctggtccg ccgtaaaggc aaaaggctgt aacggtttta
     5521 aggtatggtt aaagcggtct gcgctttcaa ggagcatctc gccgccttcc agcaacgtca
     5581 ctatcctttc catgccggga aacaaagaaa actccccatt cgccgcgatg gatgcaatgc
     5641 tggcacgcca gtaaaaatca cgttttgccg gtgggaaagt gcaaatttcg cgcgtttcac
     5701 cggcagcgtt tcgccacaga ttcaccgaca ttttacgcat atcaaagtat tccatactcg
     5761 ctcccggccc gttcttttca ttgttatcgt tcaggtaacc gtggatggtt atcggcaact
     5821 cgggtgattt acgttagtgg tgatcaggct ttttttacaa ctgtttgaga agagaaaaga
     5881 aaaccgccga tcctgtccac cgcattactg caaggtagtg gacaagaccg gcggtcttaa
     5941 gttttttggc tgaaagatta ttcagcagtt gcaggcattt taccttttgc cgctggacgt
     6001 tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg cgtcaggatg
     6061 ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg ctgttcttcc
     6121 atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc aatgatgtca
     6181 tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg gcctttcatg
     6241 atttcgtgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa catcatgtcc
     6301 tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc cggtgctgcg
     6361 gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt agaggcaaca
     6421 aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt attacggctt
     6481 tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt caattaatca
     6541 gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat ggagaaaaaa
     6601 agaaaatttg atggagagtg atgagagaat attacaacac gatgattttg cagagattat
     6661 gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt aatctgtatt
     6721 aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag tattagctta
     6781 ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta gggaataaca
     6841 catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc agcaatgttc
     6901 ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg aaggacggcg
     6961 agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc acgataccga
     7021 cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac tggcgaagat
     7081 cgttttgccc aaagggcaac gctttgccaa gttccaacgt acaggccagc gcgccaaaat
     7141 gtctgacgct gaaatgggta aacgtaccac caggctcctg atggaacacc agcgcctcca
     7201 gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg cgttgcggta
     7261 atacaccgaa ctgcggatgc aaggagccac gaattgcggt atgtagatca aggtgccaac
     7321 gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt tcgcgcgccc
     7381 gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt cggttcatat
     7441 cgctatggca ataacgtttc ccttgcttca gcgcaggagg attcccgagg atcaccagca
     7501 accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg tccagcattt
     7561 ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg ccttgcggtg
     7621 gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga acgccgttga
     7681 tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga aaattatcca
     7741 ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa ttggctcaat
     7801 acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc ggcagcagta
     7861 agacgatcga ggtagtaacg atccacccag tcattgagcg cattaaacaa ggtatcgttc
     7921 atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaataccac ccgcaaccgc
     7981 aggcacgccg ggccgccgcc attcgccatg ctttcacgta agtcaaagac ttttagttcg
     8041 ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc ggcgtgttcc
     8101 cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa cagttggctg
     8161 ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg aacttctatc
     8221 gccataaaac cgttcacccg cgtgccagta actgtgactg gcgagcgaac gcctgttggt
     8281 ggcaaaacag cacctggcgg ttactcacgg caatcacgtc attatgaaaa acgctctggt
     8341 cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg gggattcacc tgattaagcc
     8401 ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg ataacgggaa ggccgggtat
     8461 cattgccttc ttctcgcccg tagacaaaaa gtagcatacc cggttcaccg taatgaccgc
     8521 cgagacgatt gtggtttgcc gccccctcat caccgagcaa cgctacctgc ggcaacgccg
     8581 aatggacgct aaatttctct tcgtccttaa aaatcgcttt taacagcgat tcagtgacgg
     8641 gcgcttccag cgaacggtga aatttattgt tcaggttggc aacggtgaga tgcactttgc
     8701 catccagcgt atcggcagat ggcgcgatcg ttgccgcatt ggctacccac attggcgaag
     8761 cggaactgac gctggaaagc cagtgcggtg cctggcgtgc aactttttcc agtacctgct
     8821 catcgctacc actgaatccc aactgacgca gcaccggaat aaacggacgc tcgtgcggcg
     8881 gaatcacggc ctgggggaat cccgcatcgg caagggcttt cattttcagt aagccctgtt
     8941 ttgccgccag tcgcgggtta gacacctgaa aacggtgacg ggtagaggct tcattaccaa
     9001 acgacaggcc cgcgtaatga tgcgtcagcc ctaccagccc gtcgaaatta acttcccagg
     9061 cgttcatcgc accacctcat cggaaaaatc cagcccgggg ttaagcgtgg cgggcaatgt
     9121 taacgagtcc gactccaggc tcgccatagg ccatgcgcag taatctgcgg cataccaggc
     9181 gctggggcga tggttaccgg aagcaccaat gccgccgaat ggggcggtac tggcagcacc
     9241 agtaagcggt ttgttccagt taacaatccc cgcccgcgcc tccagcaaca gttgatcgaa
     9301 tttttcccgc tcgggggaaa ccagaccgca aaagagtccg aagcgagtgt tgttcgccat
     9361 ccgaatcgcc tcatcaaaag tatcataacg ccagacgcgc aaaaacggcc cgaacacctc
     9421 ttcatccggc acaccgccaa cgcccgtcat ttcaatgatc cctggtgtca gcaacgatgt
     9481 ccctgcttgt aataagcgcg gcgcaagcag ggttcgtccg cccatcgctt ccagttgctg
     9541 ccaggcagta accacctgct gtgcggcctg ttcagaaatc agcccgccaa taaacggctg
     9601 cggttcgtca tcccagttgc ccggcgttaa tcgctggctg acggcaacca gactagcaag
     9661 aaacgcatcg ccctgcgctc cgcttttgag caataaacgg cgggcgcagg tgcagcgttg
     9721 cccggctgtg acaaacgccg actgaatggt cagatggaca gccgcgtcga tatccgccac
     9781 ctcatcgata attagcggat tattaccgcc catctcaagg gcgagaattt tctccggctg
     9841 accggagagc tggcgatgca gctggtagcc ggtattggcg ctaccggtaa acagcaaacc
     9901 gtcgagatcc tccagcgcac tcagcgcctg acccgtttca cgcccgccct gcaccaggtt
     9961 cagcacgccc ggcggcaagc cagcctgctg ccataaacgc attaccgctt cgccactcca
    10021 cggtgtcagt tcgctgggtt taaagataat ggtgttacct gccagcaatg ccggaacgat
    10081 atgtccgttc ggcaaatgac cagggaaatt atacggccca aacaccgcca gcacgccgtg
    10141 cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca ctacgctgct cgccggtacg
    10201 aacgtgatac gccttaattg atatcgcgat tttattgatc atcgccgtca cttcggttgc
    10261 cgcttcccag cgcggcttac ccgtttctct ggcaataatc gcggttaatt cggctttatt
    10321 gctttccagc aatccggcaa agcgttcgac aacgacctga cgttcagcca atgagagccg
    10381 cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc tgttcgacct gagcggcatc
    10441 ggcatcattg ccttgccata acacctcgcc cgataccgga ttacgcttca cacgcgatgc
    10501 gccctggccc gttatccagt caccgttaat ccataaagtc atgctgtttt ctcctctgcg
    10561 cacaggcgca ccagacgaac gcgatcccct gcgtggcatt tgagggcatc cagttgtgcg
    10621 gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg ccagcaccac gcggaaatgg
    10681 tgataatttt cattggcgac caggcaggcc ggaaaatcgc cctgcgcagg ctgcccttct
    10741 gccacttcca ccagccgact tttacggatg gcgcgcacgc ggtcgatgtc acactcaagc
    10801 gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac gaaaaccttc tttctccagc
    10861 accgcgcggg caggcgcggt ttgcggatgt acctgaccga tgacgtcctg ggcttcctgg
    10921 gataaaaagt gggtatagat cggatgtttc ggcatcagtt ctgcaataaa tgccttttgc
    10981 ccggtgccgc agagaaaatc ggcgcggcta aaatccatcg aaaagaagcg tttaccgagg
    11041 ctttgccaga acggtgaata gccgtgttcg tcaatcaccc cgcgcatttc agcaaccact
    11101 ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa agcgcgattt cgacagcaaa
    11161 tagccgttgc cctctttgcg ccagtccggg tcgagaaaca gcgtgcacag ctcgctgctg
    11221 ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt tatagacatt cagctctttt
    11281 gaggcgtgaa ccaacgtgcc gacgcgatag ttgtaccagg gatcgttcag cccaaccgcc
    11341 acctcaatag cgcaaatccc cgccacggtg cctgtctcgc tatcttccag cacgaacaca
    11401 tagccctgct cgcttttggg cagttcacct tgccaggttt tgattgccct ttcgatacgc
    11461 gccgaaagcg tggcttcatt ggcgggaagc gacgtcaggc cgccgcccgt tttgctggca
    11521 agctgcatca gcgccgagac atctgagcgc tcaacgggac ggatgaccat catgatgaac
    11581 ctcggctaac aaagtgttcg caagcagctg caaagcgatc cagtccggtc gtcacctctt
    11641 cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt gccacccgca atcagtacca
    11701 tcacgcctgc tttcgccgct tccagagaga gctgtttcgc ttgcccggcg taatcggcat
    11761 tcagtacaca gccaatcaac aaacctaagc cgcgaacttc actgaacaaa ccatagcggt
    11821 gattaatagt attaagacgc tcaacaaacc agtcgtgacg ctgtttaacg ccattaagca
    11881 tctctggtgt gttgatgagc tccagcactt tgcctgctac cgccgaggcc agcggattac
    11941 cgccataggt ggtgccatga gtgccaacgg tcatcacgcg ggcgcactct tcggttgcca
    12001 acaacgcacc gaccgggaaa ccgccgccca gcgctttggc ggtggttaac agatcgggcg
    12061 tcacgccgta gtgcatatag gcatacagct caccagtgcg cccgacgccg gtttgtacct
    12121 catcaaaaat cagcaaggca ttgtggcggt cacacaattc acgcagacct tgtaaaaacg
    12181 cgttgctggc tggcaccaca ccgccttcgc cctagatggg ctcgacaatc accgcacagg
    12241 tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt atatgcagca tgacgaatat
    12301 ccgccggcag tggcgcaaaa tcctgtgaat aggctggctg cccacccgca ctgacagtaa
    12361 acagcgtgcg accatgaaac gcatttttga acgccacgat gccactctta tggctgccgt
    12421 agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc ttcgttggct tccgcaccgg
    12481 agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat caattttttc gccagtcgca
    12541 gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa cttactcgcc tgttcgttca
    12601 gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt caccgcaatg ccacccgcga
    12661 agtcgatata ctctttcccc tgctgatccc acaagcgcga accttcgcca cgtaccggta
    12721 taaagggtgc cggagcgtaa acaggtatca tccattcatc aaagttttca cgcgtaattg
    12781 gctgagacat agcgacctct acagtaaata attcgttatt tgtatgttaa caataagtaa
    12841 tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc cagtgataaa agtgcataaa
    12901 cggcggaggc taactggaaa tcaaggagtt ataaccaaac catatgcatt taaagtgcat
    12961 ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa taaaaaacgc aatgttatgc
    13021 agaagtaaaa tataattcta gaattgtgat cattgacgaa atttactgga aattactgcg
    13081 ccattctgac gcagcgcgca ccaaaagcgg gcattttttg cgccatcgtt gacatcatta
    13141 acaaccatcg atcaaatcac ttaacaacag gcggtaagca acgcgaaatt ctgctaccat
    13201 ccacgcactc tttatctgaa taaatggcag cgactatgaa atttgtctct tttaatatca
    13261 acggcctgcg cgccagacct caccagcttg aagccatcgt cgaaaagcac caaccggatg
    13321 tgattggcct gcaggagaca aaagttcatg acgatatgtt tccgctcgaa gaggtggcga
    13381 agctcggcta caacgtgttt tatcacgggc agaaaggcca ttatggcgtg gcgctgctga
    13441 ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg tgacgacgaa gaggcgcagc
    13501 ggcggattat tatggcggaa atcccctcac tgctgggtaa tgtcaccgtg atcaacggtt
    13561 acttcccgca gggtgaaagc cgcgaccatc cgataaaatt cccggcaaaa gcgcagtttt
    13621 atcagaatct gcaaaactac ctggaaaccg aactcaaacg tgataatccg gtactgatta
    13681 tgggcgatat gaatatcagc cctacagatc tggatatcgg cattggcgaa gaaaaccgta
    13741 agcgctggct gcgtaccggt aaatgctctt tcctgccgga agagcgcgaa tggatggaca
    13801 ggctgatgag ctggggggtg gtcgatacct tccgccatgc taatccgcaa acagcagatc
    13861 gtttctcatg gtttgattac cgctcaaaag gttttgacga taaccgtggt ctgcgcatcg
    13921 acctgctgct cgccagccaa ccgctggcag aatgttgcgt agaaaccggc atcgactatg
    13981 aaattcgcag catggaaaaa ccgtccgatc acgcccccgt ctgggcgacc ttccgccgct
    14041 aatttagcag ctctcctggc tcaaactggg tcaggagaat taaccttgag aaaaatcaac
    14101 aaactgtcag taatgatttg ttgcctgccg tcctttgtta taccgtcact gcgtttttag
    14161 ttgtctgacc acttctctat tatcaagttt gatataggaa actccacgat gaacgctgag
    14221 cgtaactttc tgtttgcctg tcttattttt gcgctggtca tttacgctat ccacgctttc
    14281 ggtttattcg atctgctcac cgatttaccc cacttacaga cactcattcg ccagagcgga
    14341 ttgttcggtt atagcctcta tattctgtta ttcatcaatg cccccctctt tctgttacca
    14401 ggaagcatac tggtgatcgc tggaggaata gtttttggcc cgctcttagg gacacaactc
    14461 tcattaattg ccgcaacgct ggcctcctcg tgctcattcc tgctggcgcg ctggctggga
    14521 cgtgatttac tgctgaaata cgtgggccat agccatacct tccaggccat tgaaaaaggc
    14581 attgcgcgta acggtattga ttttcttatt ctgacccgct taatcccgtt gtttccttac
    14641 aatattcaaa attacgctta cggattcacc accatcgcct tctggcctta tacccttatt
    14701 tcggcactca cgaccctgcc cggtattgtt atttataccg tgatggcaag cgatctcgcc
    14761 aatgaaggca ttacgctgcg ctttatttta caactctgtc tggcgggcct ggcgctgttt
    14821 attctcatcc agctcgcaaa actactctac gcccgacaca aacatgtgga tctgtctgct
    14881 tcgcgccgca gcccacttac tcacccaaaa aatgaaggat agaacgatgt cgcaacatta
    14941 ttcagtgtca tggaaaaaag gactggctgc actctgctta ctggctgttg ccgggcttag
    15001 cggctgcgat caaaaagaga atgcggcggc aaaagtggaa tacgacggac tttcgaacag
    15061 ccaaccgctg cgcgtcgatg ccaataacca tacggtaacc atgctggtgc aaattaatgg
    15121 tcgtttcctc accgacgaca ctcgtcacgg tattgtgttt aaagatggct ccaacggaca
    15181 taaatcgctg tttatggctt atgcgccccc gaaagcattt tatgaagccc tgaaagaggc
    15241 aggtggtacg ccgggcgaaa acatgacgat ggataataaa gaaacgactc atgtcacagg
    15301 cagcaaactg gatatttcgg tcaactggca aggggcggca aaagcgtatt ccttcgatga
    15361 agtgattgtt gacagtaatg gcaagaaact ggacatgcgc tttggcggta atttaacggc
    15421 agcagaagag aagaaaacgg gttgcctggt gtgtctggat agctgcccgg tcggcatcgt
    15481 cagcaatgca acatacactt atggtgcggt ggaaaaacac ggcgaagtga aattcaaagg
    15541 caatgcctca gttctcccgg cggataacac gctggcaacg gttaccttta aaatcaccga
    15601 ataaagccaa gataaaggat gatgaagata caatcgcgta aaatctggta ctaccgtata
    15661 accctcatca tcctgttgtt cgctatgctg ctcgcatggg cgctgcttcc cggcgtccat
    15721 gagtttatca atcgcagcgt tgcggcgttt gccgccgtgg accaacaggg tatagaacgc
    15781 tttattcagt cttacggcgc actggcggca gttgtctcgt tcttgttgat gattttacag
    15841 gccattgctg caccgctacc tgcgtttttg atcacctttg ccaatgcgtc gctgtttggc
    15901 gcgttctggg gcggcttgct gtcgtggacc agttcgatgg ccggcgcggc gctgtgcttt
    15961 tttatcgcca gagtgatggg ccgcgaagtg gtggaaaaat taaccggcaa aaccgtgctt
    16021 gacagtatgg acggcttttt cactcgctac ggcaaacaca ccatcctggt ctgtcggtta
    16081 ttgccttttg tccctttcga tccaatcagc tatgctgccg gtctgacttc aatacgtttt
    16141 cgctcgtttt ttatcgccac cgggcttggt caattaccgg cgactattgt ttattcctgg
    16201 gcaggcagca tgttaacagg cggtactttc tggtttgtca ccgaactgtt tattctgttt
    16261 gccctgaccg tggtgatttt tatggcgaag aaaatatggc ttgagcgcca gaagaggaat
    16321 gactgatggg tttaccgccg cttagcaaaa ttcctttcat tttacgtccg caggcgtggc
    16381 tgcatcgtcg ccattacggc gaggtgctaa gcccgattcg ctggtggggg cggatcccgt
    16441 ttatctttta tttggtgtca atgtttgttg ggtggctgga gcgcaaacgc tcaccgctcg
    16501 atccggtagt gcgatcgctt gtcagcgcgc gcattgcgca aatgtgcctg tgtgagtttt
    16561 gcgtggatat caccagtatg aaagtcgccg agcgtaccgg cagcaccgat aaactgctgg
    16621 cagtggctga ctggcggcaa agcccgctct ttagcgatga agaacggctg gcgctggagt
    16681 acgccgaagc cgcaagcgtc acaccgccaa cagtcgatga tgccctgcgt acccgactgg
    16741 ctgcgcattt tgacgctcag gcgctcaccg aactgacggc attgatcggc ctgcaaaacc
    16801 tgtcggctcg ttttaattct gccatggaca ttcccgctca ggggctgtgc cgtattccgg
    16861 aaaaacgttc ttaaggaggg aagatgcgcc attgtgggtg gttgctggga ttgttatcgc
    16921 tgttttcttt ggcaacacat gccagtgact ggcaggaaat taaaaatgag gccaaagggc
    16981 aaaccgtctg gtttaacgcc tggggcggcg ataccgcaat taaccgctat ctcgactggg
    17041 ttagcggcga gatgaaaacc cattacgcta taaacctgaa gattgttcgt ctcgcggatg
    17101 ccgcagacgc ggtgaagcgc attcagaccg aagctgctgc cggtcgtaaa acgggcggct
    17161 cggtggatct gctctgggtg aacggcgaaa acttccgcac cttaaaagag taatgccagt
    17221 cagttaagca actgactggc att
//
LOCUS       DAJTUI010000082        17083 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTUI010000082 DAJTUI010000000
VERSION     DAJTUI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17083
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.082"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..510
                     /locus_tag="OO464_002962"
     CDS             <1..510
                     /locus_tag="OO464_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR6228029.1"
                     /translation="PFREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDG
                     FTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
     gene            complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="OO464_002963"
     CDS             complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="OO464_002963"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR6228030.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1780..2868
                     /gene="hcxA"
                     /locus_tag="OO464_002964"
     CDS             1780..2868
                     /gene="hcxA"
                     /locus_tag="OO464_002964"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR6228031.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2878..3075)
                     /locus_tag="OO464_002965"
     CDS             complement(2878..3075)
                     /locus_tag="OO464_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6228032.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="OO464_002966"
     CDS             complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="OO464_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR6228033.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5543..5956)
                     /gene="entH"
                     /locus_tag="OO464_002967"
     CDS             complement(5543..5956)
                     /gene="entH"
                     /locus_tag="OO464_002967"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR6228034.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5959..6705)
                     /gene="entA"
                     /locus_tag="OO464_002968"
     CDS             complement(5959..6705)
                     /gene="entA"
                     /locus_tag="OO464_002968"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR6228035.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6705..7562)
                     /gene="entB"
                     /locus_tag="OO464_002969"
     CDS             complement(6705..7562)
                     /gene="entB"
                     /locus_tag="OO464_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR6228036.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7576..9186)
                     /gene="entE"
                     /locus_tag="OO464_002970"
     CDS             complement(7576..9186)
                     /gene="entE"
                     /locus_tag="OO464_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR6228037.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9196..10371)
                     /gene="entC"
                     /locus_tag="OO464_002971"
                     /pseudo
     CDS             complement(9196..10371)
                     /gene="entC"
                     /locus_tag="OO464_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10663..11619
                     /gene="fepB"
                     /locus_tag="OO464_002972"
     CDS             10663..11619
                     /gene="fepB"
                     /locus_tag="OO464_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6228038.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11623..12855)
                     /gene="entS"
                     /locus_tag="OO464_002973"
     CDS             complement(11623..12855)
                     /gene="entS"
                     /locus_tag="OO464_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR6228039.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGTAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12966..13970
                     /gene="fepD"
                     /locus_tag="OO464_002974"
     CDS             12966..13970
                     /gene="fepD"
                     /locus_tag="OO464_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR6228040.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13967..14959
                     /gene="fepG"
                     /locus_tag="OO464_002975"
     CDS             13967..14959
                     /gene="fepG"
                     /locus_tag="OO464_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR6228041.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14956..15771
                     /gene="fepC"
                     /locus_tag="OO464_002976"
     CDS             14956..15771
                     /gene="fepC"
                     /locus_tag="OO464_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6228042.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15768..16901)
                     /gene="fepE"
                     /locus_tag="OO464_002977"
                     /pseudo
     CDS             complement(15768..16901)
                     /gene="fepE"
                     /locus_tag="OO464_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3991 a         4863 c         4442 g         3787 t
ORIGIN      
        1 ccctttcgtg aaaagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct
       61 aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca aaaatccatt
      121 gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa tgccatggaa
      181 atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt aaaattacgc
      241 ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg
      301 actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc
      361 cgtgaactta gccagttggg atgggatgat aataaaattg gcaaagagct gggaatggac
      421 agcgacgaag tgctgcgact gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt
      481 caatattccc gcgcctggac ggttaaatag ctaaagctgg cgcaggcgtt cagctgctgc
      541 cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg ggaagggatc
      601 ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca gccactggca
      661 aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa agtatgtacc
      721 ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa tatcgcgctt
      781 ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta gcatatcagc
      841 aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta ccttgcgaat
      901 ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg tcatatgata
      961 ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat gcgccagcac
     1021 actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc taatgacaaa
     1081 aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct gccagacagt
     1141 tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc ttaataatgc
     1201 ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac gtttcactat
     1261 tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa aacaaatcac
     1321 ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc
     1381 cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt ccgttttctg
     1441 agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg
     1501 ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc
     1561 ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt
     1621 ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt
     1681 acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ccagtatttg gcaatcaaga
     1741 cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc
     1801 gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca cctgcacaat
     1861 tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg cgccattgct
     1921 gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca tattttgttt
     1981 cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg tgacgatcgc
     2041 agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc
     2101 cgtctcggtc tgccatttgt tgccgctccg acgatcgccg ccacctgcgc agcctggaca
     2161 ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat tttcgacgac
     2221 gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca acaatatctg
     2281 ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa
     2341 ccagaaacgt tgccgctaac cgtgcggctg gggatcaata atgcgcaggc cattcgcgac
     2401 gtcttgttaa acagtagcga acaggcactt gccgatcagc aaaatcagca gttaacgcaa
     2461 tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg tggtctgggc
     2521 gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac tgtcctgccg
     2581 caaaccgaga agtttcttca cggcaccaaa gtcgcttacg gaattctggt gcaaagcgcc
     2641 ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg
     2701 ccgactacgc tggcggagct ggaggtggat atcaataatc aggcggagat cgacaaagtg
     2761 attgcccaca ccttgcgccc ggtggagtcc attcattacc tgccagtcac gctgacacca
     2821 gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta
     2881 gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc
     2941 ctcgtaggtc atcggcgttt gatcgggatg attaacccgc atatgttcga catagttgtc
     3001 gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc cggcttttgc
     3061 cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc cggatgcggc
     3121 gtgaacgctt tatccggcct acaaatgcta tcaacgcact cggtcagtcc cctcgcccca
     3181 tcggggagag ggttagggtg agggggaacc ctcggcacta tgccaacatt cgccaacatc
     3241 ccccctcact ctgactttag tgcgcgcctt tcgcctgcgc tacgatctcc tcgacatttt
     3301 ccggcatcgg ctcatacggc gtttctttcg ccgttggctt cggatctttc aatgccgcaa
     3361 gtgccgtctt aatcgagaac agtgccagga ccaccacgac caccataaag aagatggtta
     3421 acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca gtatactgcg
     3481 acggaatatt gccgctgtcg atcattgcct ggaacttgtt agcaatgacc aggaagccga
     3541 ctttcgcatc cgggctaaac gctttcagcc agtcagcggt cagggtacaa atcagcagcc
     3601 aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg aacaacacca
     3661 cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca aacagcggcc
     3721 acagagtgtt aataccgccc agcggatcga ccacgctctg atggaggaag tagccccacg
     3781 ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc
     3841 caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc gtacccgcat
     3901 ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac cagaacgcca
     3961 catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc gccaacgtcg
     4021 gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca atctggttga
     4081 gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca gaagcgacta
     4141 catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca aaatacacgc
     4201 ccggatcgat gatacaggca gaaactagcg ccataatcgc cacgaaggat tccatcaaca
     4261 tcccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc
     4321 cggaagagat cagcgcatgg aagccagaca ccgcaccaca ggcgatggtg ataaacagga
     4381 acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg gtcagcgcag
     4441 gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg atagtcccga
     4501 ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag caaccacacc ggcagcaccg
     4561 ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta aagtcaaagt
     4621 acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg aaaatcagga
     4681 acaccaggcc aatcactgac acttcgccaa tgcggcccgg acgcagatag cgcaggtaga
     4741 ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc cacgggctat
     4801 gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc atgatcataa
     4861 agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg accagctcac
     4921 ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac
     4981 cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc atttgcgctg
     5041 ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg tgaccgaaca
     5101 gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc gctggcgtca
     5161 tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa aaacggtaag
     5221 cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc tgttccccac
     5281 gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact gtccagacga
     5341 ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga gatgttacat
     5401 tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa atcggagttg
     5461 aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag ttttcctgca
     5521 atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga caagtgcagc
     5581 aacgccgccc ctgttcatcg aaaacgacga tttcccaact ctgattttgc ctcccaagat
     5641 gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga tgggtggcgt
     5701 taagttccgt gcccaccaca cactgcccgt cacgggtcat cataaatccg gccatcgatc
     5761 ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac ggctgatgag
     5821 tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagaagg gtatacacaa
     5881 ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat
     5941 ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca caatatcctg
     6001 tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg
     6061 tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat
     6121 acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga
     6181 gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc cgacgctcaa
     6241 cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa tacgcgccgt
     6301 gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc gaaactggtt
     6361 catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag tctgctgcca
     6421 gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg cattgatcag
     6481 cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcca cctgcccggc
     6541 gtcggcaaca tccattactt cggtcgcaaa gggatattgc tcctgagtga acgcttgatc
     6601 aaaacctgta actttcgctc ccgcctcaac aaacgtcagc gccgtggcgt agccgatacc
     6661 tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt cacctcgcgg
     6721 gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac aaagtcgata
     6781 tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga atccagaccg
     6841 tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa cggcaggatc
     6901 acctcacgca gcgccgcttt gctggcgggg ataggtgctg gcagtaattc ttcggtcatc
     6961 accacccggc cagaacgtcc ggccacatat ttcagcgaca tcaaatgctc gtcacggctg
     7021 aaatcagcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa tgcgtcggtt
     7081 gcggtggtca tacagccaat gtgggcatat actcctgtaa taatcagctg gttacgtcca
     7141 ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc
     7201 agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg ttccggcgag
     7261 cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc gctctgctct
     7321 ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca
     7381 gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa gctgacaaaa
     7441 tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact
     7501 ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt tggaatagcc
     7561 atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta attgtttttt
     7621 atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc gatccggtaa
     7681 tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg
     7741 ctcttttacc accagataag cacagctttt ttcgcccatc agctcatttt ccatgctcac
     7801 cagtgcggca tagatcactg ccggatggct cagcagcagg ttttcgatat cttcggcagc
     7861 gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc
     7921 ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat caaaggcact
     7981 ggcattgtgc tgcggacttt tgtaatagcc acggaaggtg tacggcccgc gcgtcatcag
     8041 gcgtccaact tccccttgcg gcagtggatt tccttcggca tcggcaaccc acacttcatc
     8101 gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat catcaagacg
     8161 ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc agccaatctc
     8221 agcgggaata cgcgctgcaa gcgtggcaga aagacgtgcg ccgccgacct gtaacagttt
     8281 cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc acaggctgac
     8341 tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg ggaagcaaag
     8401 cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga cgcccagcga
     8461 tcctggcgaa ctcatagtgt agttatgagc cgccgggatc gagcacaggt agcgcgtctg
     8521 ttgtgtgaac tgacaaatct cggcgctacg acgcacgctg tagtaatagt cgttatgagt
     8581 gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat aggccacttc
     8641 atcagcaggt gatggcgtgg cagtaaaacc ctctgccgga tggttaatcg catcctgcaa
     8701 gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaagaat gctctgcaac
     8761 aaatgtgttg aggaaatcat ccccgctaaa tagcgcatgc tggcgatcgg caatcagtaa
     8821 cgccggttca atctggctgg cgtaggcgtt cagttcacta cgctgatggc tgaacagcgc
     8881 cagcaccggc gcgacgccca gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac
     8941 gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc gcaaactaca
     9001 ggcgaggtta tccgccgcct gattcagctc ccgataactc aactgccgct cgccgtcgat
     9061 aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagtg gcaaatcctg
     9121 ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg tgaatggaat
     9181 gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc
     9241 tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc cggcaaacag
     9301 acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt cgccgttacc
     9361 ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaacg gctccagttc
     9421 agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg tcggatgcag
     9481 cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa agggagttgc
     9541 gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt gtaactcact
     9601 actgcgttcg cgcagtacct ctttcatcgc ctgagtcccc agttcatgtt catggcgatc
     9661 tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat ccggctgacg
     9721 acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac gtagcagcag
     9781 ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga agttgtaact
     9841 aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg cggcgtcagt
     9901 ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc
     9961 ggcggcgcgg gcacccatct gttcaaacgt ggtttgttca ggaattgcct agcgttccac
    10021 cacgttcagc gactggctgc gggtaaaaca gcgggctgag gtctgttttt cctgacggga
    10081 gaacgactgc taggattcgg gaatatacag cgacgaaggc tgacgtggat cgaagggaat
    10141 cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc
    10201 gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt catcgaaacg
    10261 ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa agcgattggg
    10321 cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca tatcatcctc
    10381 cacaaaatga taaaggcttt aataatgatt atcacttata ttttcggccg ctaacctaaa
    10441 agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg ggttgcatct
    10501 ggttgtggtt acaatgaaaa tgagaagcat tattgatgaa ttcgcataag cgcaatgtga
    10561 tgtcctgcgc cgttttgccc cctctcccca aagggacgga ttgtgcgctt tgtcgaattt
    10621 gtcattacgc ccttaacctt attaataaca ggaagctgac ttgtgagact cgccccgctc
    10681 taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgt agttcaggcc
    10741 gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg
    10801 cagcgtattg tttccaccag cgtcaccctg accggatcac tgctggcgat tgatgctccg
    10861 gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cagatgacca gggcttttta
    10921 cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg
    10981 agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg
    11041 gattcggcgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat
    11101 tacgacgaca aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag
    11161 aaacaagcgg cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa
    11221 atcaaattac cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc
    11281 aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg
    11341 gcgaaattgc ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt
    11401 cagcttggtg gggaaaatct ggctgcgggg ttaaacggtg aatcgctatt cctgttcgct
    11461 ggtgatcaga aagatgccga tgcgatttat gccaatccac tgctcgcgca cctgcctgcg
    11521 gtacaaaaca agcaggttta tgcgctggga accgagacgt tccgcctgga ttactacagc
    11581 gccatgcaag tgctggaacg gcttaaggcg ctgttttaag cattaactgt cggacgctgt
    11641 cacctgcggc ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac
    11701 gccgataatc aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc
    11761 gcccaaacca cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca
    11821 caaaccgtta atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtatattg
    11881 cagcaacgaa ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat
    11941 ccacatcggc atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag
    12001 catcaataac cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc
    12061 cgtgccgagc gggatcgccg cgtagagaaa accaatctgt gccgctgaca tctgccagtt
    12121 gtcagccagc gccggataca gtaccagcac cgcgctcgcc atcgtcaata aaccacccag
    12181 cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcaag
    12241 cggctgcggt ggcggtggca actccggaag gcttaataac ggtaacaagg taataaacgt
    12301 gcccgccgcc gccagcccgt agttccaggc tacgccgccg gtcgccagca ataaaccgcc
    12361 aatcatgggc gaaatcaccg accccaggcg cacggtcaac atggtgatcg ccccggcctg
    12421 cattaagttt tcacgcccta ccagtgcaga tgttgccgcc agtagcgccg taacaccaag
    12481 tgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg
    12541 cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa
    12601 aatcactttt ttgcgttcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa
    12661 catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gggtcatcat
    12721 ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg
    12781 agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca
    12841 ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct
    12901 gcacatgtta acatatccaa ataagatcga taacgataat taatttcatt atcatggaag
    12961 ttcgtatatc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat
    13021 tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg
    13081 tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg
    13141 cgcggctgcc gcgtaccctt gccggtttac tggcaggcgg cgcgcttggc cttgccgggg
    13201 cgttaatgca aaccctcacc cgaaacccac ttgccgaccc cggcttgctt ggcgtgaacg
    13261 ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcacagg
    13321 aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg
    13381 gcagtcaggg tggagggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg
    13441 cggcggtgct ggaaggactg accagcggca tcgccctgct taatcctgac gtctacgatc
    13501 aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg
    13561 tgctgatccc ggttctgatc gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca
    13621 gtttgagcct cggcagtgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt
    13681 tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga
    13741 ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc
    13801 gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca
    13861 tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg
    13921 caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc
    13981 tcgccgatta ctcatcacct gtttgctgct gatttccgcc tgtgtggttg caggtatctg
    14041 gggattacgc agcggtgccg tcacgctgga aatctcgcag gtattcgccg cgttgatggg
    14101 cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat
    14161 ggcgctgttg attggcgcgg cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg
    14221 taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct
    14281 ggtggcgatg gtgctgtttg gtcaggatct gacggctatc gcgctggcag caatggtggg
    14341 cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggta tcgacacctt
    14401 tcggttgatt attatcggta tcggcgttcg cgccatgctg gtggccttta atacctggct
    14461 gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg ccggatcgct
    14521 caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat
    14581 tgccgccacc ttgctggtgc ggcgaatgcg cttgctggag atgggcgatg ataccgcctg
    14641 cgcgctgggc gtcagcgtcg agcgttcgcg tctgttaatg atgctggttg cagtggtgct
    14701 taccgctgcg gcaacggcgc ttgccgggcc gatttccttt attgctttag tcgcgccgca
    14761 cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg
    14821 agcgctgtta ctgctggcgg ccgttctctg cgcccaacaa ctgtttatgc cgtatcaact
    14881 tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca
    14941 ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg
    15001 ggatatggca aatataccgt tgcggaaaat ctgactgtag aaattcctga tggtcacttc
    15061 acggcaatta tcggaccaaa tggctgtggt aaatccacgt tactgcgtac cttaagccgc
    15121 ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca acattacgcc
    15181 agtaaagaag ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccgggcgat
    15241 atcaccgtgc aggagctggt ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc
    15301 tggcgcaaag aggatgaaga agcggtaacg aaagcgatgc aggccacggg aataactcat
    15361 ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgagc gtggatcgcg
    15421 atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat
    15481 atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga gaaaggctat
    15541 accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt
    15601 gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa
    15661 ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt agccggaatg
    15721 ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg
    15781 tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa cacgctacca
    15841 caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt acccggaccg
    15901 tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg
    15961 ttgacatttg cttttgttaa ttgctcgaca agatactgcc gattacgtaa ttcaccgttc
    16021 agttccgcaa cgtcagtaac cgctttttca atttccagtt tgcgttcaat accgtctgcg
    16081 ccgagggaaa tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc
    16141 tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca
    16201 tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt
    16261 ttgatctcca gtttattacg gacgttttct atcgactctt tcaccaccaa cgcagagata
    16321 taatcgatat acccgctcaa aacggtctgc gcctctttac tggttggcgc ggtaaaactt
    16381 agcgtccagg atgtatacag tgacggttca tcttttttct tactagcatt gtcatcaacc
    16441 gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc atcgattttc
    16501 gcctctttta attagtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta
    16561 acagactgaa acttcttgat aaacaggtta aatgcaccgc cacggtcgat attaacatcg
    16621 agatcaagaa cgcggagttt ggtaaaggtt ttctccaggt cctgccactg aatggcttcg
    16681 gcgggagtaa taacggctga gctggtccat ttttgcggca ggatgaaaga gatcagtaaa
    16741 cctgcgcagg caaatgcaaa aacgaccgcc atgaccgttt ttttggcccg ccataaaact
    16801 tcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg atactcagaa
    16861 aaatgagcgt cacttccctg tttaatattc aatgatgaca tggggtataa cctgaaaatg
    16921 agtccaatag gggaaaatct ccacggcgct cactttacca gttggtgaga attatccgaa
    16981 gctgaagttt gtaaattcct tccactgaat gcaacattca ctctatttaa gagaaatatt
    17041 catcaaacct ataaatcaaa caaataaaaa acccataatt att
//
LOCUS       DAJTUI010000083        17061 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTUI010000083 DAJTUI010000000
VERSION     DAJTUI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.083"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            241..807
                     /gene="mntP"
                     /locus_tag="OO464_002978"
     CDS             241..807
                     /gene="mntP"
                     /locus_tag="OO464_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6228043.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(804..1613)
                     /gene="rlmA"
                     /locus_tag="OO464_002979"
     CDS             complement(804..1613)
                     /gene="rlmA"
                     /locus_tag="OO464_002979"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6228044.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1779..1988)
                     /gene="cspE"
                     /locus_tag="OO464_002980"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1779..1988)
                     /gene="cspE"
                     /locus_tag="OO464_002980"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6228045.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2001..2144)
                     /locus_tag="OO464_002981"
     CDS             complement(2001..2144)
                     /locus_tag="OO464_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6228046.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(2815..3102)
                     /locus_tag="OO464_002982"
     CDS             complement(2815..3102)
                     /locus_tag="OO464_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR6228047.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3177..3320)
                     /gene="mgrB"
                     /locus_tag="OO464_002983"
     CDS             complement(3177..3320)
                     /gene="mgrB"
                     /locus_tag="OO464_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR6228048.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3479..3718
                     /locus_tag="OO464_002984"
     CDS             3479..3718
                     /locus_tag="OO464_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228049.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(3861..4652)
                     /gene="kdgR"
                     /locus_tag="OO464_002985"
     CDS             complement(3861..4652)
                     /gene="kdgR"
                     /locus_tag="OO464_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR6228050.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            4829..6202
                     /locus_tag="OO464_002986"
     CDS             4829..6202
                     /locus_tag="OO464_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6228051.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAIRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(6248..7129)
                     /gene="htpX"
                     /locus_tag="OO464_002987"
     CDS             complement(6248..7129)
                     /gene="htpX"
                     /locus_tag="OO464_002987"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6228052.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7321..9369)
                     /gene="prc"
                     /locus_tag="OO464_002988"
     CDS             complement(7321..9369)
                     /gene="prc"
                     /locus_tag="OO464_002988"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6228053.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9389..10087)
                     /gene="proQ"
                     /locus_tag="OO464_002989"
     CDS             complement(9389..10087)
                     /gene="proQ"
                     /locus_tag="OO464_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6228054.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10184..10681)
                     /gene="msrC"
                     /locus_tag="OO464_002990"
     CDS             complement(10184..10681)
                     /gene="msrC"
                     /locus_tag="OO464_002990"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6228055.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            10811..12094
                     /gene="yebS"
                     /locus_tag="OO464_002991"
     CDS             10811..12094
                     /gene="yebS"
                     /locus_tag="OO464_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR6228056.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12063..14683
                     /gene="yebT"
                     /locus_tag="OO464_002992"
                     /pseudo
     CDS             12063..14683
                     /gene="yebT"
                     /locus_tag="OO464_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            14763..16202
                     /gene="rsmF"
                     /locus_tag="OO464_002993"
                     /pseudo
     CDS             14763..16202
                     /gene="rsmF"
                     /locus_tag="OO464_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16320..16556
                     /locus_tag="OO464_002994"
     CDS             16320..16556
                     /locus_tag="OO464_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR6228057.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16661..16852
                     /locus_tag="OO464_002995"
     CDS             16661..16852
                     /locus_tag="OO464_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR6228058.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(16877..>17061)
                     /locus_tag="OO464_002996"
     CDS             complement(16877..>17061)
                     /locus_tag="OO464_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR6228059.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
BASE COUNT         4114 a         4327 c         4139 g         4481 t
ORIGIN      
        1 ttaaggttgc gccctcattt ggggagtagc cgatttccag attccggaaa tgtacgtgtc
       61 aacatactcg ttgcaaaacg tggcacgtac ggactgaaga ctttcagtca ggcgagacca
      121 tatgcacatc aatcgctatg cctgcatgaa cgcgaaaacc catcgcggct tgttgttcat
      181 gtatgcatgt ttgctggggg cgatgatgtg ttttatggat accccggtca ggacgttgtc
      241 atgaatatca ctgctactgt tcttcttgcg tttggtatgt cgatggatgc atttgctgca
      301 tcaatcggta aaggtgcccc cctccataaa ccgaaatttt ctgaagcatt gcgaaccggc
      361 cttatttttg gtgccgtcga aaccctgacg ccgctgatcg gctggggaat gggcatgtta
      421 gccagccggt ttgtccttga atggaatcac tggattgcgt ttgtgctgct gatattcctc
      481 ggcgggcgaa tgattattga gggttttcgt ggcgcagatg atgaagatga agagccgcgc
      541 cgtcggcacg gtttctggct actggtaacc accgcgattg ccaccagcct ggatgccatg
      601 gctgtgggtg ttggtcttgc tttccttcag gtcaacatta tcgcgaccgc attggccatt
      661 ggttgtgcaa ccttgattat gtcgacatta gggatgatgg ttggtcgctt tatcggctca
      721 attattggga aaaaagcgga aattctcggc gggctggtgc tgatcggcat cggcgtccag
      781 atcctctgga cgcacttcca cggttaataa gaacgctgcc agaggtgaat attaaagtcc
      841 gtctggcagt cgaacacttc ttttgctgcc agtgtttgcc agacttctgg cttcgcacgc
      901 caggcaaacg gcgtcatctg caataatgcg acggcttcat caccgcgaag acgcatcgga
      961 taacacaact ccgcactctg ctgtaatgta aaaccttcca gttgttctgc atgaggtgca
     1021 tgaagatgta cttcattgta aatcagcccc ttcagctcca tcaaatgtcg cggtcccggc
     1081 gtggcagtaa tgacccagcc gccgggcttc actactcgtg ctaattcttc tgctttacac
     1141 ggcgcgtaaa tacgtattat ggcgtccata ctggtatcgg aaaacggcaa acggtggctg
     1201 gaagcgacac aaagagtgac ctgcggatag cgtttcgccg ccgcttttat cgctaccttc
     1261 gagacatcca ggccaaacgt ggtgatttcg ggcaacgcat cggcaaatgc gtgtgtgtaa
     1321 tacccttcac cacagccaat atccagcacc gccgtggcct tttcatcaag ccgttcccgc
     1381 agttgggcga caattgcatc acgcagcggc tgataatgtc cggcatctaa gaatgcgcgg
     1441 cgtgcttgca tcatttccgc gctgtcgccc ggatcacgag accgtttatg ctgaacgggc
     1501 agcagattga catacccttc tttcgccata tcaaactgat gtcgttgggg acagatatag
     1561 ctgttttttt cacgcgaaag aggctgatgg caaagtggac aggaaaacga catgacaact
     1621 ccggcaggat actaaaggcc gcaagtgtaa cgcgaatcac gcggcgggca aatatccgaa
     1681 cgccaggagc gaagataaag catagtaaaa agcctcgcat tcgacgaggc tttatagcga
     1741 gattgaagcg tattcacact tcagatcagt ggattcgatc agatagctgt tacgttaaca
     1801 gctgccggac ctttctggcc gtcctgaatt tcgaactcaa cgttctgacc ttcagccaga
     1861 gttttgaagc cattaccctg gatagcggag aagtgtacga acacatcttt gctgccatca
     1921 gccggagtaa tgaagccaaa acctttagac tcgttgaacc acttaacctg acctttaatc
     1981 tttgccattt gaaaaattcc ttagattgtt ttcttcgccc gcaggcataa catagataaa
     2041 actgacacat tactgcatga ggcaccaata taaggctcgg cagagaagcg gtattcaacg
     2101 tcaacgtgtt tactcaggac ttctttactg aaaatgccac acataaacag aactgtacct
     2161 cgtttaaccc gaaatctttt atcacataca acgttaatta tggcaagcca tttttaaaca
     2221 tatctcgatc agacgcacaa atctcgatac gtcttccact ttttttgcac acttatgcaa
     2281 cggaatacgc gccgatatat cattgtgctt aaccttgcca gttcaggcag atacttaaac
     2341 tggcgtagtt tctaacatag ttcaataagt tcagttgttc caggccagag ggagaagtta
     2401 ttacatagtg cgtgcaatat cacattttct gctatgcaat gaataaaaag ttatatcact
     2461 ttttctcata aaacagtcag ttaacggcta tcaattaccc taaagagaag tcaatcccca
     2521 aagggattgt aaatttaaaa taagaaaaat tgatgagtga gcaaaaaaaa tcaagagaga
     2581 aacgtttcgc taataaataa taccacgctt aatcacaaat ctgatgtaaa catcctaata
     2641 aattaatgag gaaaatatct ttttctgata tcccgttacg caaataaata atatgataat
     2701 tatttgaagt cagaccagag ttttaatttg aagcagttgc accatgacag tgcgtataat
     2761 caggatgaat tgagtaaccc ttcaacgacg gggttactca agattgagga aggattatcg
     2821 ttcggcgacc aggcgaataa tatcttcgtc atcaaccgat ttttgactct caatggtctt
     2881 tttatctgct ttctctggtt cgtttgcttc gcacaaacga cgtaacagtg cattttgacg
     2941 tttttgctga tccagcaacg cctcaagcag ttcaatctgt tcgttagtcc gtgaactggc
     3001 acgattgata aaaaaccaca agatgagccc gataagaaga accaccaccg atacaaccaa
     3061 agacgcaatg ttcatcacgc ctgaatttac aacttcgttc atttcaccac ctctgagtag
     3121 aggcgctatt ctaccactgc tggagaggaa gaaaatctag tgctgaaaaa atgatatcac
     3181 cacgggataa actggttaat ggcacaaatt ccgctgaaaa attgtacatc ctgatcgcac
     3241 atcatgttga atacctgcgc ccaaagcagc aagcaagcca acaccacgac aaccagaacg
     3301 acccatcgaa actttttcac tccaatctcc gcttcaccta ccttatgtca ttaaactagc
     3361 atgcgttagt taaacagcgc ctaactatgt cgatattcgt gctttttcgg aatgagtagc
     3421 ttgcttcagc gcatattgcc gctacgatta agcgaacata aaaagagaag agattgtaat
     3481 gcgattcatc attcgcacag ttatgctgat agcactggta tggataggat tattactcag
     3541 cggatatggc gttttgattg gaagcaaaga gaatgccgca ggattaggtt tgcaatgtac
     3601 ctatctgact gccagaggca ccagcacggt acaatacttg cacactaaaa gtggtttctt
     3661 ggagataaca gattgcccac tgttacgtaa aagcaatatc gtggtcgata atggttaagg
     3721 gtaataaata accagattct cactcatgag taaaatattc ttatagcaag cgcgtttaat
     3781 ataacgcacc ttcaacttta tttttctcat gctaaataaa gaacctgtct gtttgatatt
     3841 ccgacaggtt cttactctca tcagaacgga tagtcgtgat aacccatttg ggcagaaatt
     3901 ttgcgcgctg cggtatgcaa cattgcgaca tattcctgta aacgctcttc agagaaacgc
     3961 aacgtcggga aggagatgct caaaccggca atgaccacgc caaagcgatc aaataccggt
     4021 accgcaatgc attgcagccc ttcttcctgc tcttcgttat cttctccata cccctgctcg
     4081 cgcacctggt ccagaacggg taataacgct tctgtactgg tgatggtccg ctcggtactg
     4141 cgtttatact ccacgccctc aagaatttgc ttcacttcat cgcgatcgcg ccatgccagc
     4201 agtaccttac caatcgcggt gctgtacagc ggattacgac gcccaatccg tgaatacatg
     4261 cgcaaattgt acatagagtc aattttgtga atgtaaacaa tactgtcttc gtccagtgcg
     4321 ccgaggtgga tagtttcttt ggtcaggcgg gagatctcac gcatctggat atctgcgcta
     4381 cgaattaaat cgacgttttg taacgcgcga gcgcccagtt caaacaattt cagggtcagg
     4441 gaatatttct ccgactcccc ttcctgcgcc acataaccta aggttttcat ggtctgtaaa
     4501 aagcgataaa cggtgctttt tgacatcatg acgcgctgcg acagctcggt tatccctatt
     4561 tcgcgctctt cacccagcgc ctgcaaaatg ccaaaaactt ttagcacgga agatacagaa
     4621 tcaggctgtt tatccagatc tgcgttagcc atttatcacc tcattgcgag tgttttataa
     4681 aaatcagaac tgttttttat tataatttcg caccagggtg gtcgcaatcc atcttttgcc
     4741 ggttagttac aattctgcga catccaccgt gaatatcagt gctagaatca tacccctgtt
     4801 gattattcac caaagatata aaattcctat gccaaaagtt caggccgacg gcctgccatt
     4861 gccccagcga tacggtgcga tattaaccat tgtgattggt atttcgatgg ctgtccttga
     4921 cggcgcaatc gccaacgtcg ccctgccaac aattgccacg gaccttcatg ccacaccagc
     4981 cagttccatc tgggtagtga acgcctatca aatcgccatt gtcatctccc tgctctcgtt
     5041 ttcgtttctg ggcgatatgt ttggctatcg acgtatttat aaatgcggtc tggtcgtttt
     5101 tctgttgtct tcactgttct gcgccctttc tgattcgctg caaatgctca cccttgcgcg
     5161 tgtcatacaa ggtttcggcg gtgcagcgtt gatgagcgtt aataccgcac ttatccgcct
     5221 gatctatcca caacgttttc tggggagagg gatgggcata aactcgttta ttgttgccgt
     5281 ctcttctgct gccgggccga caattgctgc agcaatcctc tccatcgcat cctggaaatg
     5341 gttattttta atcaacgtac cgttaggtat tatcgccctg cttctggcga tacgttttct
     5401 gccacccaat ggttctcgcg ccagtaaacc ccgtttcgac ctgcccagcg ccgtgatgaa
     5461 cgcgttaacc ttcggcctgc ttatcactgc gttgagtggt ttcgctcagg ggcaatcgct
     5521 gacgttaatt gctgcggaac tggtggtaat ggttgttgtt ggtattttct ttattcgccg
     5581 ccagctttct cttcccgtac cgctgctacc ggtggattta ctgcgtatcc cgctgttttc
     5641 actttctatt tgcacatctg tttgctcttt ctgcgcacaa atgctggcaa tggtttccct
     5701 gcccttttac ctgcaaaccg tgctcgggcg tagtgaagtc gaaacaggtt tacttctgac
     5761 accgtggccg ttagcaacga tggtgatggc tcctctggca ggctatttga ttgaacgcgt
     5821 acatgcagga ttgctgggtg ctttagggtt gttcatcatg gctacggggc ttttttccct
     5881 ggttctgctg cccgcgtcac ctgcggatat caatattatc tggccgatga tcttatgtgg
     5941 cgctggattt ggcttattcc agtcacccaa taaccacacc attattacct ccgctccgcg
     6001 cgaacgtagc ggtggggcca gtggcatgtt aggaacggct cgtctactgg gtcagagtag
     6061 tggcgcggcg ctggtggcgc tgatgctaaa tcagtttggt gataatggta cgcacgtctc
     6121 gctgatggct gcggctattc tggcagtgat tgctgcctgt gtcagtggtt tacgtatcac
     6181 tcagccacga gccagggcat aaaaaaagcg cgtcgatcag gacgcgcttt ttagtattta
     6241 cttcatatta cttcaggtat tcacccgtac gcagagcttc aatacgttta tccagcggcg
     6301 ggtgagtcat gaacaactca ctgagcgatt tcgacttacc gttaatgcag aaagccatca
     6361 tgctggttgc ttcttgcggt tcatagctgg ttttcaggcg ctgcagcgcg gcaatcattt
     6421 tctcgcgacc aaccagtttt gccgaaccgg catctgcatg gaattcacga tgacgcgaga
     6481 accacatggt gataatgctc gccagaatac caaacaccag ttccagaacc gttgcaaccg
     6541 caaagtagat cagcgggttg ccgttgctct cttcaccttc atcacgattt ccgcccataa
     6601 aacccgcggc aagctgcgcc agaatacggg aaataaagat aacgaaggtg ttcaccacgc
     6661 cctgaatcag cgtcatggtg accatatcac cattggcgat gtggctgatt tcgtgagcaa
     6721 ttaccgcctc ggcttcatcc gggctcatgt tctgcagcaa accggtgctg acagcaacca
     6781 gagaggcatc acggcgcgca ccggttgcaa aagcgttgat gtccggcgca tggtagatag
     6841 ccacttgcgg catagcgatc cccgcctgac gagcctgggt tgctacagta ttgaccagcc
     6901 aacgttccct ttcgttacgc ggttgctcga tcacttcccc gccaacagat cgtaatgcca
     6961 tccatttgga catcagaagc gaaacgaagg aaccaccaaa accgaacagc aaggccatga
     7021 tcatcagccc ctgaacgctg ctcgactgta tccctgtcag gctcagtacc agcccgaaaa
     7081 cgaccattac ggccaggttc gttagcagga agagcgcgat tcgcatcata attttctttt
     7141 tacctcagtt taacaaaacg caatatgcga tacccacatc gtatgggtta cgcgactatt
     7201 ttcaagtctg gatagtgcgt aagtcaccag aaagacacaa ctttacattt tgtagcatct
     7261 gatttacggc atcttgccgc tgttaaaaaa tcaggcacaa tttcttgtgc ctgattgata
     7321 ttacttgacg ggagcgggtt gttccgcggg tctggctttt tcaagcttcg ccagatcgag
     7381 tgcgatattc accgtctcat ccagataagg atccggctcc tggtaatctt tcgatagatc
     7441 atccagcttc ttcaactccg gtttaccttc tcgtttaaag cgttcgttca aacgcgccag
     7501 acgcgtcgca tcatcttcat tattctcttt ctcacgcaca gcgtagttca gagaaacgat
     7561 attgcgcttg tccttcatag cgttgaagcg cgcgatatcc ttcatgatgt tctggaactc
     7621 aggatctttc gcgatacgcg cattatgttc cttcagcagc tccggttcaa aggcagttaa
     7681 atctcctgat ttcacataag tcgcggcatc gatgctatcc cacggcagcg cgttatcttc
     7741 gaatttctca cccgtttccg tttcttcatt acccgtcggc atgatgatgt ctggtgttac
     7801 gcctttacgt tgcgtactgc cgccgttaac gcgatagaat ttctggatcg tgtactgcac
     7861 agaacccagc gctggccatt caggacgtaa catctgatcg taaatacggt tcaatgaacg
     7921 gtattgctga acggtgcctt taccaaacgt cggttcaccc acaaccagcg cacgaccgta
     7981 atcctgcatt gccgcggcaa agatttctga agccgaagca ctgaaacggt caaccagtac
     8041 caccagcggg cctttataga acacctgccc gtcggtatcg ctgtcttcac gaaccttgcc
     8101 gttgttatcg cggacctgaa caatgggacc cgcaggaata aacagacctg agagcgatac
     8161 cgcttcggtc aacgccccac cgccattgct acgcaggtcg atgatcacac tgctgacatt
     8221 ctgtttttcc agtttctgca gttgaacttt gacatcgtct gtcaaaccca catagaagcc
     8281 cggaatatcc agcacgccga ctttctcttt accgacggtc ttcaccgaca ttttaaccgc
     8341 gcggtcttcg agacgaatac gttcacgggt caacgttaca gtacgggtct tggtcccttt
     8401 accagcaggt aaaatttcca gacgaacttt actgcccttc ggccctttaa ttaaggcaac
     8461 cacatcatca agacgccagc caatcacgtc aaccatcggc ttgcctgttt gaccaacacc
     8521 gacaattttg tcaccaacgc tgatagcttt actcttcgct gccggaccac ctgccaccat
     8581 cgaattgata acggtgtagt catcatccat ttgcagcact gcgccaatac cttccagtga
     8641 caaactcatt tcagtgttga actgttcggt attacgcggg gaaagatagt tggtatgcgg
     8701 atcgatttca cgcgcaaacg ccgtcattgc cagcgagaaa acatcttcgc tgttggtttg
     8761 cgccagacga cgaatggcaa atttgtagcg gcgtgtcagg gtttcacgaa tttctttatc
     8821 cgtttttcct gtcagcttca ggcttaactc gtcgaatttg actttactgt cccactgcgc
     8881 gttcaactca gcctcgtttt tcggccaggg cgctttgctg cggtcaaggt tataagtgtc
     8941 gttgccggtg aaatccatcg gcttttccag taccgacaaa gcgtactggt aacgttcaaa
     9001 acggcgcttt tgcgccagat tgtagagatc gtagaaaacg tcgagtttgc ctgaacgcag
     9061 ttcatcgcct aactcggttt tctttttcgc gaactgttca acatcgcttg ccagcagcac
     9121 attgtggctg taatcgagca gattcaggta gcggtcaaag attttggccg aaaatgcctg
     9181 atcgaggtcg aactggcgat aatgagaacg ggtgaagcgc gacgttacgc gctcacttac
     9241 cgtcgcatgc tgcgtctctt cctttaatac cggaatttga tcagcacgcg tgatatcttc
     9301 tacagcgaag gtctggcctg ctattgcaag caggccagct aacgcggtaa gcctaaaaaa
     9361 catgttcatg cctggcccgg cctccgtttc agaacaccag gtgttctgcg cgcacaatca
     9421 aagacatacc cgaattcagc tggacgcgga cgccgtcttt ggtgatttct aatacggtgg
     9481 catccatcgc gttttgaccc gctttcacct tcagggcttg tccgacagtc agagctgaaa
     9541 tgtcagaaac cggggtgtgc tgttcttcgc gaggtgcttt tactgttttt ggcgctttct
     9601 ctaccggctt ttgcgcacga ggtttacgtt cagcgccttc tttgcggcgt ggcgtggtcg
     9661 gacgtggctt gcgttcgcgg cgcggtgcgt cttctttctc accagcagct gcggcagctt
     9721 cgcgtttttt cgcttgctgt tcagcacgct gtgcctgaac acgcgctttc gcttcttcaa
     9781 gctgcttgcg agcatgctct acatgttgct cgtccagctc accgcatggg ttgccgtcaa
     9841 gatcgacacg cgttgcgccg ggtttaacac cgtaaagata acgccagctc gaagtgtaga
     9901 gacgtaaagc ggatcgtaat tgcgttttgc tcaggttcat ttccccggca acacgatcga
     9961 ccaaatcctg aaaaatacca attttcagcg gacgcgcttc accttccgca ctgaaacagt
    10021 ggggaaaacg ttcggccaga aacgcgatta cttctttact gctattcaac ttaggttgat
    10081 tttccatgaa atttcctgat tacaacggac gtagccaaca agcgcaggca tgaacaggcg
    10141 tcattataat gacgctacca gtaaatgcta cgttatccgt tgattatcct gcgacgctcg
    10201 caaagaattt tttgtaatcc gtcgttgcaa gcactttttc aagctgtgcc acaagctgac
    10261 gtaagccttg ctcgtcctcg tctgtaaagc gagcgaagac agtactatcg atgtcgagaa
    10321 caccaataat ctgatttttc accaccagcg gcagaacaat ttcagaatta ctcgccgcat
    10381 cacaggcaat atgcccgtca aacgcgtgaa catcctcgat acgctgcact tgattgcggg
    10441 caaccgctgt gccgcacacc ccgcgcccga cgggtatccg gacacaggca attttgccct
    10501 gaaatggtcc gagtaccagt gtatcgtcct caagcaaata aaaacctgcc cagttaacgt
    10561 cagtgagacg ctcgtataac aacgcactgg tgttcgcaag cgttgccaga aaactggttt
    10621 ctcccgccat cagcgcgtta aagtcgcgat ttaaatccgc gtaaaattct gttttgttca
    10681 ttacttaatc acttggttgt cttacctgga actgccagcc tattaaaata agcattaaat
    10741 gcgttaatgc tcaagatcat tcccatcatg ggttaagatt aatgttaatt cttattacat
    10801 ttggcacgtc atggctctta acacaccaca aattacgccg acaaaaaaga taacagtgag
    10861 ggcaatcggc gaggaactgc cgcgtggtga ttaccaacgt tgcccgcaat gtgacatgct
    10921 gtttagcctg cccgagataa attctcatca aagtgcctat tgtccgcgct gtcaggcaaa
    10981 aattcgcgac gggcgcgact ggtcgctaac gcgcctggcg gcaatggcct tcaccatgct
    11041 gttgcttatg ccgtttgcct ggggcgaacc gctgttgcat atctggctgt taggcattcg
    11101 tattgacgcc aacgtcatgc aaggcatctg gcaaatgacc aaacagggcg atgcgataac
    11161 gggatcaatg gtctttttct gcgttattgg tgcccccctc attctggtgt cctccatagc
    11221 atatttatgg tttggtaacc gactgggaat gaatttacgt ccggtactgc tgatgcttga
    11281 gcgacttaaa gagtgggtaa tgctggatat ctacctggtc ggcattggcg ttgcttctat
    11341 aaaggtacag gattatgccc atatccaggc gggtgtcggc ttgttctctt ttgtggcgtt
    11401 ggtgatttta acgacggtga cgttgtcaca tcttaatgtt gaggagctgt gggagcgata
    11461 ttatccgcag cgccccgcta cgcgtaggga cgagaaactt cgtgtctgtc ttgggtgcca
    11521 ttttaccggc tatcctgatc agcgtggtct ctgcccgcgt tgccatatcc cgctacgcct
    11581 gcgtcgtcgt catagtttgc aaaaatgctg ggcggcgctg ttagcgtcaa tcgttttgtt
    11641 gttaccagcc aacctattgc ctatttctat catttatctg aatggtggcc ggcaggaaga
    11701 tacgattctt tccggaatta tgtcgctggc aagtagcaac attgcggttg cgggaatcgt
    11761 gtttatcgcc agtattctgg taccgtttac taaagtgatc gtcatgttca ctttactgtt
    11821 gagcattcat tttaaatgcc agcaaggttt acgcacacgc attctgttac tgcggatggt
    11881 gacctggatt ggacgctggt cgatactcga cctgtttgtc atatctttaa ccatgtcgct
    11941 gattaatcgc gatcagatcc tcgcttttac tatgggaccg gctgcgtttt attttggcgc
    12001 tgccgtaatt ttgactattc ttgctgtgga atggctggac agccgcttac tttgggatgc
    12061 acatgagtca ggaaacgccc gcttcgacga ctgaagcgca gattaaaaat aaacgccgta
    12121 tctcaccttt ctggctgctg cctttcatcg cgctaatgat tgccggttgg ctgatttggg
    12181 acagttatca ggaccggggt aataccgtca ccatcgactt tatgtcggcg gatggtattg
    12241 ttccgggccg tacgcctgtt cgttatcagg gcgttgaagt cggaacagtg caggatatca
    12301 gcctcagcga cgatcttcgt aagattgaag tcaaggtcag catcaagtcc gatatgaaag
    12361 atgcgctgcg cgaagagact cagttctggc tgatgacgcc aaaagcatcg ttggcaggtg
    12421 tctccgggct ggacgccctc gtcggtggga actatatcgg catgatgccg ggtaaaggta
    12481 aagagcagga tcactttgtc gcgctcgata cccaaccgaa atatcggctg gacaatggcg
    12541 atctgatgat ccacctgcaa gcccccgctc tcggttcgct gaacagcggt tcattggtct
    12601 atttccgcaa gatcccggtg ggaaaagtct acgactatgc catcaatccc aacaagcaag
    12661 gcgtggtgat tgatgtcctg atcgagcggc gttttaccga tctggtgaaa aaaggtagcc
    12721 gtttctgaaa cgtttccggc gttgatgcca acgtcagtat cagtggcgcg aaggtgaaac
    12781 tggaaagtct ggcggcactg gttaacggtg cgattgcctt cgattcacct gaagagtcga
    12841 aacctgccga ggcggaagat acctttggtc tgtatgaaga tctggcccac agccagcgtg
    12901 gagtaataat aaaactggaa ctgccgagtg gagcaggatt aaccgccgac tcgacgccgt
    12961 taatgtatca ggggctggaa gtcggacagc tgactaaact ggatttaaat cctggtggta
    13021 aagtcactgg ggaaatgacc gttgatccct gcttcgtgaa aatacccgca tcgaattacg
    13081 caacccgaaa ttatccctta gcgatgctaa tctcagcgcc ctgctgaccg gaaaaacctt
    13141 tgagttggtg cccggcgatg gcgagccacg caaagagttc gttgttgtgc cgggcgaaaa
    13201 agccctgctg caagagccgg acgttctgac actgaccctg acagcaccgg aaagttacgg
    13261 tattgatgcg ggtcagccgc tcattcttca cggcgtgcag gtaggccagg ttatcgatcg
    13321 taaactcacc agcaaaggcg tcacctttac cgtcgccatc gagcctcagc atcgagaact
    13381 ggtaaaaggc gatagcaaat ttgtcgtcaa cagccgtgtc gacgtgaagg tggggctgga
    13441 tggcgttgag tttctcggtg ccagcgcctc agaatggatt aacggcggga tacgtattct
    13501 gccgggcgat aaaggcgaga tgaaagccag ctatccactg tatgccaatc tggaaaaagc
    13561 gctggagaac agccttagcg atttacccac cacaaccatg agtttgagtg cagagacgct
    13621 gccggatgtg caggcaggat cggtagtgct gtaccgtaaa tttgaagttg gtgaagttat
    13681 taccgtgcgt ccgcgagcta acgcgttcga tatcgatctg cacattaaac cggaatatcg
    13741 caaccttttg accagcaaca gcgtgttctg ggcagaaggc ggggcgaaag ttcagctgaa
    13801 tggtagtggc ctgaccgtac aggcatcccc gctctccaga gcattaaagg gggccattag
    13861 cttcgataac ctcagtggtg ccagcgccag tcagcgtaaa ggcgacaagc gaattctgta
    13921 tgcttccgaa acagcggccc gtgcggttgg caggcagatt acgcttcacg ctttcgatgc
    13981 cggaaaactg gcggtcggga tgccaattcg ctatctcggt attgatatcg ggcaaatcca
    14041 gacgctggat ctgattaccg cacgcaatga agtacaggca aaggcggtgc tctatccgga
    14101 atatgtccag acctttgctc gcggtggtac gcgcttctca gtggtcacac cgcaaatctc
    14161 ggcagctggc gttgagcatc tagatactat cctccagccg tatatcaacg tcgaaccagg
    14221 ccgtggcaat cctcgtcgcg actttgaatt agaagaggcc accattactg attcgcgtta
    14281 cctagatggt ttaagcatta ttgttgaagc gccggaagcc ggttcgttag gcatcggtac
    14341 gcctgtgctg ttccgtggtc tggaagtagg tacggttacc ggaatgacgc tggggacatt
    14401 gtcagatcgc gtgatgattg cgatgcgcat cagtaaacgc tatcaacacc tggtgcgtaa
    14461 caattccgtc ttctggttgg catcgggtta cagtctggac tttggtctga cgggcggcgt
    14521 agtgaaaacc ggcaccttta accaatttat ccgtggcggc atcgccttcg ccacgcctcc
    14581 gggtacgcca cttgcaccga aagcccaaga aggcaaacac ttcctgttgc aggaaagtga
    14641 accgaaagag tggcgtgaat ggggaactgc gcttcccaaa taatgcccac tgctccggcg
    14701 tgcctgcgcc ggagcgctta tgctaaactg cgcgcctgtt tttttgccag tggtacatgc
    14761 tcgtggccca acacaccgtt tatttcccgg acgcgtttct gacacaaatg cgcgaagcta
    14821 tgccttcgac gctctcattt gatgattttc ttgccgcctg tcagcgcccg ttgcgccgca
    14881 gcattcgcgt taatacgctg aaaatctccg ttgctgattt cctgcaatta accgctcctt
    14941 atggctggac gcttacgcca attccgtggt gtgaagaagg tttctggatt gaacgcgaca
    15001 atgaagatgc attgccattg ggtagtacca ccgagcattt aagtggcctg ttttatattc
    15061 aggaagccag ttcaatgttg cccgtcgccg ccttgtttgc tgacggtaat gcaccacagc
    15121 gggtgatgga tgtcgctgct gcgccaggct caaaaacgac gcaaattgcc gcgcggatga
    15181 ataacgaagg ggcaatcctt gccaatgagt tttccgccag ttgggtaaaa gtgttacatg
    15241 ccaatatcag ccgctgtggc atcagtaatg ttgcgctcac acattttggt ggccgcgtgt
    15301 ttggtgcggc agtgccagaa atgtttgatg ccattttgct ggacgctccc tgctccggcg
    15361 aaggcgtggt gcgtaaagat cccgatgcgc taaaaaactg gtcacccgaa agcaatcagg
    15421 aaatcgcagc tacccaacgg gaactgattg acagcgcctt tcatgcatta cgtcctggcg
    15481 gtacgctggt ttactcgacc tataccttaa accgggaaga aaacgaagcc gtttgccggt
    15541 ggctgaaaga gacttacccc gacgaagtag agtttttacc acttggcgat ctcttccctg
    15601 gtgcaaacaa ggcgctgacc gaagaaggtt ttttgcatgt tttcccacaa atttacgact
    15661 gcgaaggctt cttcgttgct cgtctgcgta aaactcaggc gatccccgcc ttacccgccc
    15721 caaaatacaa agtgggcaat ttcccgttca gcccggtgaa agatcgcgaa gccggacaaa
    15781 ttcgtcaggc ggctgcaagt gttggcttaa actgggatgg aaacctgcga ctctggcaac
    15841 gcgacaaaga actgtggttg ttcccggtag gcattgaagc cctgatcggt aaagtccgat
    15901 tttctcgctt agggattaaa cttgccgaaa cgcataacaa aggttatcgc tggcagcatg
    15961 aagcggttat tgcccatgcc tcccccgaca atgtaaacgc ttttgaactg acaccgcagg
    16021 aagcgtagga gtggtatcgc gggcgcgatg tttacccgca agccgcgcca gtggcggatg
    16081 acgtgttggt tactttccag catcagccga ttggtttagc caaacggatt ggttcgcgac
    16141 tgaaaaacag ctacccgcgt gaactggtgc gggacgggaa actttttacc agtaacgcat
    16201 gacagcgcac aaaaaaagcg cactttttga ctggcacatt cgccggcctc aactaggctg
    16261 aaaaattgtg cgatcggact ggtcgtacta caatcagcag ctaaatggag agcacgaaga
    16321 tgaaaaccag tgtgcgcata ggcgcttttg aaatcgacga cggcgaatta cacggtgaat
    16381 cgccgggtga tcgaacgtta accattcctt gtaaatctga ccccgattta tgtatgcaac
    16441 tggatgcctg ggatgctgaa accagtatcc ctgccctgct aaatggcgaa cactctgtcc
    16501 tttaccgtac ccgttacgat caacaatctg atgcctggat tatgcgtctt gcctgatcca
    16561 aaaagaaccc gtcggcatgg cgggttattt gtcctggtta ttcccccgtt gtaaaatctc
    16621 tcctaaactt aacggtacgg caccacactt cggggatgaa atgttcgcgc tggtactttt
    16681 tgtttgctac ctggatggcg gttgtgaaga tattgttgtg gatgtctaca acacggaaca
    16741 gcagtgtctt tattctatga gcgatcaacg gatccgccat ggcagttgtt ttccgattga
    16801 ggattttata gatggtttct ggcaaccagc acaggagtat ggtgattttt aattattgca
    16861 gttgcacaag agtcagttag cccccaaaga cagcaccggt atcaatataa tgcaggttgc
    16921 caatatccac gcgatgtcgc aacggtgtat gaccaaacca gaaatgatca gcacctgtaa
    16981 ttccctgccc ttttgggcgt tcacctaatc gcgagcggct ccacaagacc tgatgcaaat
    17041 caacgtcctt ttgccattca t
//
LOCUS       DAJTUI010000084        16987 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTUI010000084 DAJTUI010000000
VERSION     DAJTUI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.084"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..374)
                     /locus_tag="OO464_002997"
     CDS             complement(<1..374)
                     /locus_tag="OO464_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Specifically methylates the ribose of guanosine
                     2251 in 23S rRNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6228060.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAV"
     gene            complement(554..2995)
                     /gene="rnr"
                     /locus_tag="OO464_002998"
     CDS             complement(554..2995)
                     /gene="rnr"
                     /locus_tag="OO464_002998"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6228061.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3034..3459)
                     /gene="nsrR"
                     /locus_tag="OO464_002999"
     CDS             complement(3034..3459)
                     /gene="nsrR"
                     /locus_tag="OO464_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6228062.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3664..4962)
                     /gene="purA"
                     /locus_tag="OO464_003000"
     CDS             complement(3664..4962)
                     /gene="purA"
                     /locus_tag="OO464_003000"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6228063.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5066..5263)
                     /locus_tag="OO464_003001"
     CDS             complement(5066..5263)
                     /locus_tag="OO464_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6228064.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5345..6349)
                     /gene="hflC"
                     /locus_tag="OO464_003002"
     CDS             complement(5345..6349)
                     /gene="hflC"
                     /locus_tag="OO464_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6228065.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6352..7611)
                     /gene="hflK"
                     /locus_tag="OO464_003003"
     CDS             complement(6352..7611)
                     /gene="hflK"
                     /locus_tag="OO464_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6228066.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7697..8977)
                     /gene="hflX"
                     /locus_tag="OO464_003004"
     CDS             complement(7697..8977)
                     /gene="hflX"
                     /locus_tag="OO464_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6228067.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9053..9361)
                     /gene="hfq"
                     /locus_tag="OO464_003005"
     CDS             complement(9053..9361)
                     /gene="hfq"
                     /locus_tag="OO464_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6228068.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9447..10397)
                     /gene="miaA"
                     /locus_tag="OO464_003006"
     CDS             complement(9447..10397)
                     /gene="miaA"
                     /locus_tag="OO464_003006"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6228069.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10390..12237)
                     /gene="mutL"
                     /locus_tag="OO464_003007"
     CDS             complement(10390..12237)
                     /gene="mutL"
                     /locus_tag="OO464_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6228070.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12247..13584)
                     /gene="amiB"
                     /locus_tag="OO464_003008"
     CDS             complement(12247..13584)
                     /gene="amiB"
                     /locus_tag="OO464_003008"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6228071.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13603..14064)
                     /gene="tsaE"
                     /locus_tag="OO464_003009"
     CDS             complement(13603..14064)
                     /gene="tsaE"
                     /locus_tag="OO464_003009"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6228072.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14036..15583)
                     /gene="nnr"
                     /locus_tag="OO464_003010"
     CDS             complement(14036..15583)
                     /gene="nnr"
                     /locus_tag="OO464_003010"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6228073.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15582..16721
                     /gene="queG"
                     /locus_tag="OO464_003011"
     CDS             15582..16721
                     /gene="queG"
                     /locus_tag="OO464_003011"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6228074.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16704..16757)
                     /locus_tag="OO464_003012"
     CDS             complement(16704..16757)
                     /locus_tag="OO464_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228075.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3852 a         4840 c         4272 g         4023 t
ORIGIN      
        1 atcaccgcat gaacgcctgc ggcgtccgca ctacgcaggc acgcgccgag gttgtgcgga
       61 tcggttacgc cgtcgaggat cagcaggaac ggttgatcga gcgaagcgat cagatccggc
      121 agatcgtttt cctgatactg acgtcctggc ttcacgcggg cgataatgcc ctgatgcacg
      181 gcaccgtcgc ttttctcgtc gagatattgg cggtttgcca actggataac cacgccctga
      241 gactcaaggg cgtgaatcag cggtaacaga cgtttatctt cacggccttt taaaataaag
      301 acttcctgaa aacgttcagg ggcgcgctcc agcagggcct gcactgcgtg gatgccgtaa
      361 atcatttcgc tcattaatgt actcgttgtt tacgttttgc ctgatgcact acgtttatcc
      421 ggcctacatg atctctgcaa tatattgaat ttgcgtgatt atgtaggccg gataaggcgt
      481 tcacgccgca tccggcatga acaaagcgca ttttgtcagc aatctaaccc tcttctttta
      541 aagagggtat tgatcactct gccacttttt tcttcgccgc acgcttcgct ttggtcgctg
      601 cggctatttt ctgcgttttc gccgatggct ttttcgcttt tctcgcgtct ttcttcgccg
      661 ctttcggctt cgtttttttc tcaccgcgga aggcgctgtc tggctcaaag tttacctttt
      721 taccgacctg acaacgcttg ccgccttttt tgcctgcatc gccttttttc gctttctcgc
      781 gcgccgtttt accgacgttg cgcggtgcgc gttcactgga gatcagacta aagtcgattt
      841 tgcgctcgtc catattaacc gcttcgacgc gaacttccac gcgatcgccc aggcgataag
      901 tctggccgct ggattccccc atcaggcgtt gccctacctg gtcaaagcga tagtagtcat
      961 tgtccagcga agagacatgg accagaccat caatgaacaa gtcgtccaga cggacgaaga
     1021 agccaaagcc agtgacgctg gaaattacgc ctttaaagac gttacctacc tggtcgagca
     1081 tgaagtcaca cttcagccag tcagcgacat cgcgcgttgc ttcgtcggca cgacgttccg
     1141 ccatcgaaca gtgctgaccc agttgcaaca tctcttccat cgaataatgg tagccgccgg
     1201 tttcagtggt gttgccctga tgcccctgct ctttcgccag cagatattta atggcgcggt
     1261 gcagcgtcag gtcaggataa cgacgaatcg gcgaagtaaa gtgcgcatag gactgcaatg
     1321 ccagaccgaa gtgaccacgg ttttctggat cgtaaatcgc ctgtttcatc gagcgtagca
     1381 gcatggtttg cagcatttct gcatcaggac ggtcggcaac cgactccagc agttccgcgt
     1441 agtcacgcgg ttccggcttg ttaccacccg gcagctccag ccccagctcc gccagcactg
     1501 aacggaaaga ggtaatcgct tcggtgctcg gcttgtcgtg aatacggaac agtgccggtt
     1561 ctttcgcttt ctcaacgaaa cgcgccgccg agatattcgc cagaatcatg cactcttcaa
     1621 ttaatttgtg cgcgtcgtta cgctgggtct gttcgatacg ttcaatacgg cgttcagcgt
     1681 tgaaaatgaa cttcgcttct tcgctctcaa atgagatccc accgcgttct tcacgggctt
     1741 tatccagcac tttatagagg ttatgcaact cttcgagatg cttaaccagc ggggcgtact
     1801 gctcgcgcag atcctgatcg ccctgcagaa tatgccagac tttggtgtag gtcagacgcg
     1861 cgtgagagct catcaccgct tcgtagaatt tgtagcccgt caggcggcct ttcgacgaaa
     1921 ccgtcatctc gcacaccata cacaggcggt ctacctgcgg gttgagcgaa cacaggccgt
     1981 tagagagcac ttccggcagc atcgggataa cctgcgaagg gaagtacacc gacgtgccac
     2041 ggttacgcgc ttctctgtcc agcggcgttg gcggacgcac atagtagctg acgtcggcaa
     2101 tcgcgaccca taaacgccag ccgccgccgc gttttttctc gcagtaaact gcatcgtcaa
     2161 agtcacgggc gtcttcgcca tcaatggtga ccagcggtaa atcgcgcaaa tcgacacggc
     2221 ccgcttttgc ttcttccggc acttcttctt tcagccccgc aacctgttgc tcaacagcct
     2281 gcggccagat atacggaatt tcatgggtac gcagagcgat atcaaccgcc atgccggtgc
     2341 ccatattgtc gcccagcact tcgacgattt tacccaccgc tttggtgcgg cgagtcggac
     2401 gctgagtcag ttcgactacg accacaaagc ccatccgcgc gcccatgatc tgatcgggcg
     2461 ggattaagat atcgaagctc agacggctat cgtcaggaac cacaaagccg acgcccgctt
     2521 cggtaaagta gcgaccaaca atctggctgg tttttggcac cagtacgcgg acaatacgcg
     2581 cttcacgacg acctttacgg tcagcaccca gcggctgcgc cagcacctga tcgccatgaa
     2641 tgcaggtttt catctgctcg ctggagagat acaaatcatc tttacgccct tcaacccgca
     2701 gaaagccgta gccatcacgg tgaccaataa cggtaccttt caccaggtcg aggcgttccg
     2761 gcagcgcata gcactgacga cgagtgaaga ccagttgacc atcgcgctcc atcgcgcgca
     2821 ggcggcgacg caggccttca agctgctctt cgccttcaat gtgcagttct accgccagct
     2881 catcacggct ggccggtttt tcacgtttgg ttaaatgttc gaggataaat tcccgactag
     2941 ggatgggatt cgcgtatttt tcagcttcgc gttcctggaa aggatcttgt gacatctcgg
     3001 ttcctccgtt gtcatctctg atgaagattt tcgtcactcc accagcaata atttataaag
     3061 cggttgattc tcttcaacca aatcggcaag cgtgtagtta tccagttccg taagaaaact
     3121 ttgcacggcc ttagaaagcg cctgtttcaa ccgacaggca ggtgtaatgt ggcaaaactc
     3181 actgctgcaa ttcaccagcg ataagggctc cagctcgcgc accacatcac caatacgtat
     3241 cgcactcgcc ggtttaccca ggcgaatgcc gccatttttt ccacgaacag cagtcacgta
     3301 gccggcacga ctaagttgat tgattatttt gaccatatga ttacgggaga cgccgtagac
     3361 gtcagtcact tcagaaatac tggtcatccg cccttctggc aatgacgcca tgtagatcag
     3421 cgcacgtaat ccgtaatcag tgaaactcgt taactgcaca tcaacctcaa aagggaaatc
     3481 gggaaaaaat acatttatat tgatgataaa ccagccacaa gctgtgtcgc taatttattt
     3541 cagttcgggg aaggaaaaaa gcgaggattt aacactgtgt cggatagcgt aatggcttaa
     3601 ccgacacagc aatgtatcac actgttagcc cggcaggcaa aatatctgcc aggcgtacca
     3661 gaattacgcg tcgaacgggt cgcgcagaat catggtttca gtacgatccg gaccggtaga
     3721 gatgatatcg atcggcacac cggtcagctc ttcaatacgc ttgatgtagt tcagcgccgc
     3781 ctgcggcagg ccgctacgat ctttcacgcc gaaagtggat tcagaccagc ccggcatggt
     3841 ttcgtaaatc ggctctacac ctttccagtc gtcagctgcc agcggagtgg tagtcacttc
     3901 gcgaccatcc ggcatacggt aagccacgca gagtttaacc tctttcaggc catccagaac
     3961 gtccagttta gtcaggcaga agccagacag ggagttcagc tgtaccgcac gacgaacggc
     4021 aacggtgtcc agccagccgg tacgacgacg acgacccgta gttgcaccga attcgttacc
     4081 ctgcttgcag aggaactcgc cagtttcatc aaacagttcg gccgggaaag gacctgcacc
     4141 tacacgagtt gagtaagctt tgaggatacc cagaacgtaa tcaacataac gcgggcccag
     4201 gccggaaccg gtcgccacgc caccagcagt ggtgttggaa gaagttacgt acggataagt
     4261 accgtggtcg atatccagca gcgtaccctg cgcaccttcg aacatgacga aatcgccacg
     4321 ctgacgcgcc tggtcgagca ggtcagaaac gtcaaccacc atagaagtca ggatgtcggc
     4381 aacagccatc gtatcatcca gaactttctg gtaatcaacc gcttcagctt tgtagtagtt
     4441 aaccaactgg aagttgtgat attccatcac ttctttcagt ttttcagcga aggtttcttt
     4501 gtcgaaaagg tcgccaacac gcagaccgcg acgtgccact ttatcttcat aagcaggccc
     4561 gataccacga ccggtggtgc cgatcgcttt cgcgccacgc gctttctcac gcgcgttatc
     4621 cagagcaacg tgataatcaa ggatcagcgg acatgcttca gacagcagca gacgctcacg
     4681 aacggggatg ccacggtctt ccagttcttt catctctttc atcagcgcgg ccggagacag
     4741 cacaacaccg ttaccgatga tgctggttac attctcgcgg agaatacctg atggaataag
     4801 atggagaacg gttttttcac cgttgattac gagagtatgg cctgcgttgt gaccgccctg
     4861 gtagcgtaca acatatttag cccgttcagt cagaagatcg acgatcttac ctttaccttc
     4921 gtcaccccat tgggtgccca gtacgacgac gttgttaccc atttttcaaa atcaccgttt
     4981 gcttaaaaat ggattctacc atcgcttttt cagagttaca gcactttttg cactcaaaaa
     5041 tgacctcgat cagtctgttt tttgttcagc caatcgtttt cctcaacatg tagtagacca
     5101 caacgcccgc aaccacaagt ccaccgccaa aacgacgtaa aatattatcg ggcaaattgg
     5161 tcatcgcaga gatcatcttc ttccatgcct tcgggtaaag catcggccct aaaccttcca
     5221 gtaccaaaac cagggcaagc gccagccaga ttgtcgaatt cattcattat ccttatagaa
     5281 aaagaaaacc accgacatcc ctgaggatgc ggtggcttta ttgacctgta ccgcagtcgt
     5341 tatattaacg cgttgcggaa gtcggcgtct tcatgtagcg gaagaaatcg ctatccgggc
     5401 tcatgaccat cacgtcctga ttgccagaga agctgttctc ataagcacgc aggctacgga
     5461 tgaatgcgta gaagtccgga tctttactga atgcatcagc aaacagtttg gctgcttcgg
     5521 catcgccttc accacgcata atgcggccct gacgctcagc ttctgccagc gttctggtca
     5581 cttcatagtc ggcagtcgcg cgcagttttt ccgcttcttc ctgaccttgt gaacggtgac
     5641 gacgcgctac cgcttcacgc tcggcgcgca tacggttgta gatcgcttca gacacttcgg
     5701 tcggcaggtt gatctgcttg atacgcacat cgacaacttc aatacccagc gccgccatac
     5761 tgttcgggtt gatgaccgga actttgccct tcgtctcagc cgttacgcgc tctgccgctt
     5821 cggcaatggc gttatctgct gccggggtag taacttcatc ttctgtaccc gcagaaccgg
     5881 agttcagcgc gtcacgtact tcgagggtca gacgaccacg ggaatcggtg acgatatctt
     5941 tcacgtccag gcgaccaatt tcagaacgca gacggtcaga gaacttacgt ttcaacagca
     6001 cttccgcttg cgaaatgtcg ccaccacccg ttgccaggta gtaacggctg aaatcgctga
     6061 tgcgccattt gatgtaagag tcgacgatca ggtctttctt ctctttggtc acaaagcggt
     6121 cggcctggtt gtccatggtc tgaatacgtg cgtcgagcat tttcaccgtt tcaatgaacg
     6181 gtatcttgaa atgcagacca ggctcataaa ccaaaggttt gttgtcatcg tcacgcagta
     6241 ccttaccaaa acgcagcgta ataccgcgct caccttcttt gacgacaaag acagacatgt
     6301 aaagcactac cagcacgatg atgataatcg cgataactga cttacgcatc gttattcccc
     6361 ctgacgctgg tagtcgttac gctgcgcgtt ggcgcggcgt tggtccataa tatcgccctg
     6421 actggtggac gacgtgttgc ttgctccact ggttgtggag gaagaggctg gcggcagacg
     6481 cagcagattg ctggcaccgt tatcgctctt cgccgcaggg gcgttaccac ctttcagcat
     6541 ctggtctaac ggcagaacca tcaggttgcc acctttatcg ttaaccagca ctttgcgggt
     6601 gttacccaac actttttcca tcgtctcgat atacagacgc tcgcgagtaa tttccggcgc
     6661 ggctttatat tccggcagaa gtttagcaaa gcgcgccact tcaccctgag cttccaggat
     6721 ggtctgggcc ttgtacgcac gcgcctcttc gaggatacgt tgcgcctgac cgttcgcacg
     6781 cggctgaact tcgttggaat acgcttctgc ttcacgaatg tattgctgtt cgttttcacg
     6841 cgcggcaatc gcatcgtcaa acgccgcttt tacttcttcc ggcggacgag cagcctggaa
     6901 gttgacgtcc agcagcgtga tacccatgtc atacggacga atcgtctctt ccagttcgcg
     6961 ctgagtatcg ctacgaatca cggtacgacc ttccgtcaga atgcggtcca tggtgtattt
     7021 accgataact ccacgcaggg cgctgtcggt agcctgacgc aggctgtcat ccgggctggt
     7081 cacgctatac agatattttt ccggattggt gacgcggtac tgcacgttca tctcaacgcg
     7141 cactacgttc tcgtccgacg tcagcatcac accagaagcg gccagttcac gcacggcttc
     7201 cacgttcacc ggtttgactt cgtcgataaa cgtcggtttc cagttcagac ccggctcaac
     7261 cagatggctg aatttaccaa agcgtgttac cacgccgcgt tcggcttctt taatggtata
     7321 gaaaccactg gccgcccaga taatgacaat cgctgccgct gcgatggtaa cgacacgacc
     7381 gccaagctgc gggcgcgggc cttgcgatga actgccaccg ccagatccgg tgcctttacc
     7441 gccgcccaga ccaccgagct ttttgctcag tttgcggaag atatcatcta aatcaggtgg
     7501 cccttgatcg cgaccgcctt tgtttccatt tccctcagag ttgccgccag gtttgctgct
     7561 tccccacggg tcgcggtctt gtccgttatt accgggctga ttccacgcca tgtttgtgct
     7621 ccatatttgt tatgcggtga tccctgttgt ctttcgacgc cagaggatat gactccacgc
     7681 ttcagacgct acgccgttag atcaggtaat cgatcaacgc cggttcttgt ttacagaggc
     7741 gacgccagtc aacgatcggc atacgaactt gcagacttac gctgccgtcc tcctccatcc
     7801 actctttttc tattgcctga agctgataaa aacgactcct cagacgccct tcctgcggtg
     7861 gcagacgcaa tgtatgctgc gccacctcgc cggaaagccg ctccgtcaaa gcctgaaaaa
     7921 gctgtggtat ccccgctccg gtctgtgcgg aaagccagac acggatcggt ttgttctctt
     7981 cgtcccgatc aatacgcggt tcgaaatctt ccagcatatc gatcttgttc atcaccagca
     8041 gggttgggat ctcgtgagcg tcgatctctt caagaaccgt attcaccgct tcgatgtttt
     8101 cttgtacacg cacatccgcc gcatcaatga cgtgcagcag taatgtggct tgccgcgtct
     8161 cttgtaacgt ggctttaaat gccgccacca gatcgtgcgg caggtggcga ataaacccta
     8221 cggtatctgc aagtacggtt tcaccgacat ctgcaacgtc aatacgccgc aacgtcgggt
     8281 cgagggtggc aaacaactgg tctgccgcgt agacccgcgc ttcggtgatg cgattgaaaa
     8341 gggtagattt accggcgttg gtatatccca ccagcgaaac agtaggaacg tcggctttga
     8401 tacgcgattg ccgcccctgc tcacgctgct tttcaactct ttccaggcgc gactgtatct
     8461 gcacgatgcg attacgcaac aaacgacggt cggtttcgag ctgggtttca cccggaccac
     8521 gcaaacctat cccgcctttc tgtctttcaa ggtgggtcca gccacgcacc aggcgcgtag
     8581 ccagatggcg cagctgcgcc agctcaacct gcaacttacc ctcatgggta cgcgcacgtt
     8641 gggcgaaaat atctaaaata aggccggtgc ggtcgataac acgacactcg cacaaacgct
     8701 ccaggttacg ctcttgcgcc gggctcaggg catggtcaaa aagaacgacc gaagcaccca
     8761 tcgctttgac agcttccgca atttcaactg ctttaccttc acctacaaaa tactttgggt
     8821 gcggcgcttt acggctaccg gtaatcacct gcaatgcttc gacaccggcg gaagagacca
     8881 gagattcaaa ctcctggagg tcttccatat ctttgtcttg cgtaaaatag atgtgtacca
     8941 gtaccgcctg ctcaccagca tcataacggt caaacaagcg tataaccctc taaatagatc
     9001 agcggggaac gcaggatcgc tggctccccg tgtaaaaaaa cagcccgaaa ccttattcgg
     9061 tttcttcgct gtcctgttgc gcggaagtat tctgcgcgct gctaccatga tggtagttac
     9121 tgctggtacc gccaccggcg ttgttactgt gatgagaaac cgggcgagat gggacaacag
     9181 tagaaatcgc gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg atcacgaact
     9241 gatcaaaaga ctcgatttgc ccttgcagct taataccatt caccaaataa atagaaactg
     9301 gaacacgttc ccgacgcagt gcgttcagga acggatcttg taaagattgc cccttagcca
     9361 ttctctcttt tccttatatg cttatttgta ctttgaacct ttcgattctg aaaaaattgc
     9421 gcacgatacg tctcaattgt acacattcag cctgcgatag caccaacaac ctgtaatact
     9481 tcgtcacgcg cctgttctgg tttttcactg tcaagccagt gaaccccttc ccaaccacgc
     9541 agccaggtta tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt
     9601 tcatcgtatg agatttcgcc ttcaaggtaa gaccacatct ggcgataacc cacgcaacga
     9661 atggaaggca agtccgtatg caaatctcct cgggcaaaaa gcgcccggac ttctgcttca
     9721 aaacctgaag ccaacatctg atgaaaacgc tgctcaatgc gttgatggag cagttcacgg
     9781 ctcgccgggg cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc
     9841 gtcagttccg ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt
     9901 tgtggatcat ttggatgaat ccttgctgcc gcaaccggat ctacctcctg aagttgacga
     9961 tgcaatgact cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg cacttccggg
    10021 tctgccgacg gtagcggcga caacccttcc agcaatgcct tgaaatacaa catcgtaccg
    10081 cccactaaca gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg
    10141 cggcgaaaat cagcagccga gtaagcctgc gacggatcgc gaatatccag caatcggtgc
    10201 ggcgcggcga gtaactcttc agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa
    10261 ataaggacag aatcaacgct tatcaactct actggtaaaa ttttacgcag ctcaatggct
    10321 aacgccgttt taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc
    10381 ttactgatat cactcatctt tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa
    10441 cagaccaccc ggcggcgttt tcacaagttg cggacataac cgttccacgt ccgccagcag
    10501 ggttatggcc tgtgccattg accactgcgc atgttcgctc atcagatttc gtgcaatcca
    10561 ctgcgcaata ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta tcagttcagg
    10621 aatcaagatt tgtaaatttt gttggcgtaa gggtaaaggc acggccctga tggtcacatg
    10681 ctgtgcatct gactggaaat caatacccaa ttccgctagg gcagactgcg ctttttctaa
    10741 tgccgatttt tcttcgccag aaacttttag ccgcaacgga atcagcagcg gctgggcgca
    10801 aacgggcgct tcacccggcg tcaattgtgc ctgacgcagc caacgttctg ccactggcaa
    10861 ggataaaagt gaaatgttgc catcgcgctc cagcaacgca cagtcggaat ggacgatagt
    10921 cagtacccga ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc
    10981 ttttggtttt tgcatcggcg cgggcgtttg caaaagctgg cgatacactt caccttgctg
    11041 tttctggtag cctggctgcg cattcggcca gggggcagcc ggacgactgc ctgaggcggg
    11101 cgcaggagag tagcgcggag ctaccggctc acgagctgcc ggttctgcaa agtgattgcg
    11161 ccccgccgcc acgcggtttt ccgggatggc acgcggtgcg ggttgtggtt catcgtccag
    11221 cggtagcggc gtttccagtt gctgttgtag cacgctcagc acgccctgat agataaaatc
    11281 atgcaccaga cgcgactgat ggaaacgcac ttcgtgtttg gcggggtgta cgttgacgtc
    11341 cacctgatgc gggtcgatct ccagatacaa cacaaatgcc ggttgctgat cggcccccag
    11401 tttgtcttcg caggcctggc ggatcgcatg attgatcagg cgatcgcgca tcatgcgacc
    11461 attcacgtag caatactgaa tttccgccag tgcgggcgta gtgtgatttg gatcggccac
    11521 ccagccgcgc agggtgagat cgccgtgttg ccattcaatc gccagcgctt gttcgagaaa
    11581 agcggtgccg caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg
    11641 gtactgacgc acaattttac cgttatgcga caggttgatc gtgacgtcga aacgcgccag
    11701 cgcaatgcgg cggatgattt catcaatatg gctaaattcg gttttctcgg tgcgcaggaa
    11761 tttgcgccgc gccggggtgt tgtagaacag atccagcacc tccagcgtcg ttcccaccgg
    11821 atgcgctgcc ggtttaaccg tcacgtccat gtcgcgtcct tcggcatagg cctgccaggc
    11881 ttcctgctgt tctgcggtgc gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc
    11941 cagcgcctca ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca gagaggcgat
    12001 tttactggtg gcatgacgag ccagcgccag cgccagctca tcttttttga taccgcagcc
    12061 gttatcacga atgcggataa gtttcgcccc accgcgttcg atatcaatat cgatacgcgt
    12121 cgcacctgca tcgaggctgt tttccactag ttctttgact accgacgcag gtcgctcgac
    12181 cacctcacct gcggcaatct ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa
    12241 tcgtccttag tttggcagcg tgcgatctgg cgtcgtcacc gtactggcag tttgtgccgt
    12301 tgcaccctgc ggcgcagatt gcatcggatg cgcaaggaag taattgcgca ggcctttgta
    12361 aatggcttct gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt
    12421 gttgctgata aaaccggttt cgaccagtac tgatgggata tccggcgagc gcagaacgcc
    12481 aaggctggcg tgttctggtc gacgtttatg tatttcgcca atgcgttgca actgactgat
    12541 catactggtc gctacatcat accctacccg ctgggaatga ccgaactgta aatccagcac
    12601 cgcctggctt aaataggggt cagactgact gttcgccagc acatcacccg ccccacccag
    12661 cagctccgac agtttctcgt gctgctccag ccagctggcc atttcactgt tggcgcgacg
    12721 gttagagagc acccatacgg aagcgccagt cgcactacgg ttcggtgcgg catcagcgtg
    12781 aatcgacacg aggaaattgg cgttttgctt atgtgccaca tcactgcgcc ccatcaccga
    12841 gataaagtaa tccccgtcac gggttaaaac gcctttaaac atcggatcgt cattgagcaa
    12901 agtacgcaat ttacgcgcga tggcgatggt gacatttttc tcccgcgtac caccggggcc
    12961 gatagcgcca gggtcctgac cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc
    13021 agtgttagcc gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga taacacccgt
    13081 agtgcggtta ctttccgttt taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac
    13141 aaccgcaggc gtttcaacgc gtttcgcaac cacgggcggc ggaggaggcg gtggcggcac
    13201 atcggcgtta atcgtaaaga cgacagtgta attgctgcca ttctgccgct tcaccgcttc
    13261 ggttttaccg ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt
    13321 tccagagcga atcgccttca ccagattatt gccgctgaac aacaacggca gtccctgaat
    13381 catgcccgtt tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc taaacgcata
    13441 atcaggatcg ccaataaaac tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg
    13501 aatatcagag agcgtcgcgg cacccaccgg cgtgcacagc agcagcagcg tcgctaccaa
    13561 ccaatttctg atgcgataca tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc
    13621 cagcaacaat tcacccgcag aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg
    13681 ataatcaatg tgtatttcga catccgggtc aggaagaaca cctgtacctt gttgtggcca
    13741 ctccaccagg cagatggcat cgttggcaaa ataatcgcgg atccccataa actccagctc
    13801 ctcgggatcg gcaaggcggt acaaatcaaa gtgatagacc attaagttgt cgagcgtata
    13861 gggttcgacc agcgtataag tggggctttt gacattaccc tgatgaccca gagcctgtaa
    13921 aaagccccgg ctaaaggtgg ttttacctgc gcctaaatcg ccatacagat agattacggt
    13981 tgcgccatcg caggctttcg ctacccgctc gcccaggtct aatgttgcct gctcatcagg
    14041 gagcggaatt actcgattca tcatggtttt tatcagtcac ttccgggtta acaatacgct
    14101 gtagcgtgga aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc gccgccagta
    14161 cgtcagctgc cgcaccgtgc gcgacacagc ctgcacaggc tgcatcatac ggcgacagtt
    14221 tttgcccaag caatgcgcca ataataccag agagcacatc gcccatgccg ccgctcgcca
    14281 tgcctgcatt tccggcatca ataatgccta aagcgtcagg atgggcggcg accacggttc
    14341 cggcaccttt cagcaccgct acgccgccat aacgttgtac cagacgtttg gcgcaatgta
    14401 agcggtcact ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc tcgctaggat
    14461 gcggcgtgat cacgcgattg tgacgcttat cgggattgat tgccagcagg ttcaatgcat
    14521 cggcatccca caacatcggt ttgcgaaaat tctcaacttt ttgcagggct tttttccccc
    14581 actcttgctg gcccagaccg ggaccaatca ccaccacatc ggcccattcc aggctttcgg
    14641 taagagagtc catagtcagt tcatgcacca tcaattctgg tcgtgcagtc agcagcggcg
    14701 caatgttctc actgcgggtc agtactcgga ctaaaccagc accagcacgt agcgccgctt
    14761 cccccgtcat acgaatagcc cccgccgtgc cgtgatcgcc accgataatc accagccgcc
    14821 cgtgatcgcc tttatgcgaa gtcgggcgac gcggtttcag ccagtgagaa agttgttctg
    14881 ccgaaaaccg ctgaattttc gtctcctgac ctgccagcca gctatccagc cccagtgagt
    14941 caaaatgcag ttgtcctgta acatcccgcg cttttccagt gagcaagcct agtttcagcg
    15001 caataaaagt gatggtgtga tcggcgttga tcactgcgcc tggcgtagcg ccggtttcag
    15061 ccagcaggcc ggaagggata tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt
    15121 cgattaactg gctaatggat tcgcggggcg cttgctgcaa gccggtaccg agcagcgcat
    15181 caacaatcag atctaccgat tcgggccaga caatattcga agcatggatc tcgccacccg
    15241 cgtttaacca tgcttcgcgt gccagcgcgg cctcttccgg caacggtttg tcgctctcct
    15301 gggccaacaa cgtgacctca atgccgaccg ctttggccag tcgcgcgacc acgtagccat
    15361 cgccgccgtt attaccatga ccgcacagca ccagccagtg gcgggcgtca ggatacgccg
    15421 aacgacacac ctggaatgcg gcctcgccag cgcgaagcat cagctcatag agtgtgagcc
    15481 ccagcacatc tgccgcctcg cgttctccgc ggcggatatc gtcggcgtac cagacggtgt
    15541 gtggtatact tacggggttt ttcttcattg tatggtccgt catgtcagag cccctcgatc
    15601 tcaatcagtt agcgcaaaaa attaaacagt gggggctgga actgggcttt cagcaggtag
    15661 gtattaccga taccgatctc agcgagtccg agcccaaact gcaagcatgg ctggacaaac
    15721 aataccacgg cgaaatggac tggatggcac gtcacggtat gctgcgcgct cgcccccatg
    15781 agttattacc cggtacgctg cgtgtaatca gcgtgcggat gaattacctt cctgctaacg
    15841 ccgcatttgc cagcacgctg aaaaacccca aactcggcta tgttagccgt tatgcgctgg
    15901 gccgtgacta tcacaaactt ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc
    15961 aacattgtgt ttcgctgaat tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc
    16021 cgttagctga aaaagctggg ctcggctgga caggtaagca ctcacttatc ctcaatcgcg
    16081 aggccggttc gttcttcttt ttaggcgaat tgctggtcga tattccgctg cccgtggatc
    16141 aaccagtcga ggaaggatgc ggcaaatgcg tggcctgtat gacgatttgc ccgaccggtg
    16201 ccatcgtcga gccatatacc gtcgatgctc gccgctgtat ctcttatctc accatcgaac
    16261 ttgaaggggc gctcccggaa gagttgcgac cgttaatggg aaaccgtatt tacggttgcg
    16321 atgactgcca gcttatctgc ccgtggaatc gctattcgca actcactaca gaagacgatt
    16381 tcagcccgcg taagccgcta cacgcaccgg aactcattga gttatttgcc tggagcgaag
    16441 agaagttttt aaaagtcacg gaaggatcgg cgattcgccg tataggtcac ctgcgctggc
    16501 tgcgtaatat cgccgtggca ttaggcaatg ccccttggga tgaaacgatt ttgacagcgc
    16561 tggaaagtcg taaaggtgag cacccacttc tcgatgagca catagcgtgg gcgattgcgc
    16621 agcaaataga gagacgaaat gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc
    16681 tggttcgggt gattgaaaaa gggttaccgc gtgacgcctg attcatccac agcttgtgaa
    16741 taaaaataaa aacgcattgc aattcaagac gaataaaatc gtcaagtgat cgactcgaca
    16801 aaacggaatc ttgattttta acttatttat caataggtta aaaaaatacc attcaccgtg
    16861 cgaagtttct ttgaaattaa gattacagcc tgaggctgtg gataactctg tttacaagag
    16921 tttgtcagag acaacaaaaa ccagcaataa cgcatcgcgt cgctgtagat attttattga
    16981 gagaaga
//
LOCUS       DAJTUI010000085        16389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTUI010000085 DAJTUI010000000
VERSION     DAJTUI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.085"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="OO464_003013"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="OO464_003013"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6228076.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OO464_003014"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OO464_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6228077.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2339..2554
                     /locus_tag="OO464_003015"
     CDS             2339..2554
                     /locus_tag="OO464_003015"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228078.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="OO464_003016"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="OO464_003016"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6228079.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /gene="galP"
                     /locus_tag="OO464_003017"
     CDS             4449..5843
                     /gene="galP"
                     /locus_tag="OO464_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6228080.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="OO464_003018"
     CDS             5920..6417
                     /locus_tag="OO464_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6228081.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="OO464_003019"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="OO464_003019"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6228082.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="OO464_003020"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="OO464_003020"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6228083.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="OO464_003021"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="OO464_003021"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6228084.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9102..9665
                     /locus_tag="OO464_003022"
     CDS             9102..9665
                     /locus_tag="OO464_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6228085.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="OO464_003023"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="OO464_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6228086.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="OO464_003024"
     CDS             complement(10257..11237)
                     /locus_tag="OO464_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6228087.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="OO464_003025"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="OO464_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6228088.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="OO464_003026"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="OO464_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6228089.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /locus_tag="OO464_003027"
     CDS             12540..12830
                     /locus_tag="OO464_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6228090.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="OO464_003028"
     CDS             12838..13431
                     /locus_tag="OO464_003028"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6228091.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="OO464_003029"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="OO464_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6228092.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14874..15837
                     /locus_tag="OO464_003030"
                     /pseudo
     CDS             14874..15837
                     /locus_tag="OO464_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15882..16385
                     /locus_tag="OO464_003031"
     CDS             15882..16385
                     /locus_tag="OO464_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR6228093.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4059 a         4220 c         4124 g         3986 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct
    12061 cattgtgatt tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact
    14641 gaataaatat cgacgacatt tattatatat atttagtgaa tatttactaa tcattcattg
    14701 attataaata tgtacaacct ggcgcacaaa tttaatattt aatttctgac tttatttcaa
    14761 gttgtgataa acatagacaa gttgtcagac aggttaacca atttgtcgca caaatcactg
    14821 ataagcacaa ttagatatca accaatttcc atccttcaac agggagtttt tttatgaaaa
    14881 tgaggtcatt tacccgctca ttagtctgcg catccttgct ggctctggtt agcactggcg
    14941 taaacgccgc agaaaaagtg gcgttaaagt tagcacacaa cctggaaaga agtcatgttg
    15001 tacatcagag ttttgaagag ctggcgaaag aggtaaaaca gctgtcaaaa ggtaatatgg
    15061 tgatccgtat ttatcccagc agccagatgg gtaatgcgcg agaaactatg gaattgttgc
    15121 aaaatggcgc actggatatg accaaaggtt ccgccagtga tctggagtct tttgataata
    15181 tttatgctat ctataatttg ccgttcttat taagttgttt ttggtgaagt tggtaaagaa
    15241 attatggact caacaaaaga caaaggcttt tttgctttat cagcttatgt agcgggtact
    15301 cgcagtttct atgcgaaaaa accgattaca aagccagaag atcttaaagg actgaaaatt
    15361 cgtgttcagc caagtccgac caccatcaaa atgattgaac tgatgggtgg ttcacccact
    15421 ccaatttcat ttggcgaagt ttatactgcg atgcagcaag gtgtagtaga tggcgcagaa
    15481 aataacgtgc cttcctggat gcagactcga catattgaaa ttgccaaagt tttttctgaa
    15541 gatgaacacg cctcaattcc tgactttttg gttatctcca ccaaaacatg gaataaatta
    15601 accccagaac agcaacaaat cctcgaaaca gcggcgaaga aatccgaagc ttatcaacaa
    15661 aaattgtggg aaaaaattga cgctgacaca cgtgctcagg cgaaagctat ggggggtgaa
    15721 attgtaaaag tggataaagc gccattccgt aaagccgtgc aacctctgtt tgacgacttc
    15781 aaaaaagatc caaaacaagc tgctttgtta gagaaattcg acaacgctgc tcaataatat
    15841 tccgggccag cctctggccc tgattttcca ggatgcttac aatgatattt aaccgcctga
    15901 aacttgcggt agaccgcgtc attgccacgt tttctgtcgc cgtaatgctt gctctggttg
    15961 tatgcgtagt ctggcaagta tttagccgct acgtacttaa ccagccaagt acattaaccg
    16021 atgagctggc acgctttttg atgatctggg taggattatt aggtgctgca tataccgtcg
    16081 gcgcgcagcg acatctctct attgatttat ttgctctcgc cctgaataag cgcaaacaat
    16141 tacttcttag tatagtcatt aacgttctca ttttaggttt tgccggttcg gtcattgtga
    16201 ctggtggcct gaaattaatt gataaaacac tggcaacatc acaggtatct gccgctatgc
    16261 aaataccgat gggatacgta tatatcattc tgccattaag tggagtggta atgatgtttt
    16321 atgccttgtg ttttattaat caaagcattc aacaactgaa acaacctgcg caggaggcca
    16381 gttaatgga
//
LOCUS       DAJTUI010000086        15928 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTUI010000086 DAJTUI010000000
VERSION     DAJTUI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.086"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            442..1500
                     /gene="citC"
                     /locus_tag="OO464_003032"
     CDS             442..1500
                     /gene="citC"
                     /locus_tag="OO464_003032"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6228094.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKPPA"
     gene            1515..1811
                     /gene="citD"
                     /locus_tag="OO464_003033"
     CDS             1515..1811
                     /gene="citD"
                     /locus_tag="OO464_003033"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6228095.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1808..2716
                     /gene="citE"
                     /locus_tag="OO464_003034"
     CDS             1808..2716
                     /gene="citE"
                     /locus_tag="OO464_003034"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6228096.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2727..4259
                     /gene="citF"
                     /locus_tag="OO464_003035"
     CDS             2727..4259
                     /gene="citF"
                     /locus_tag="OO464_003035"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6228097.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4263..4813
                     /gene="citX"
                     /locus_tag="OO464_003036"
                     /pseudo
     CDS             4263..4813
                     /gene="citX"
                     /locus_tag="OO464_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4788..5666
                     /gene="citG"
                     /locus_tag="OO464_003037"
     CDS             4788..5666
                     /gene="citG"
                     /locus_tag="OO464_003037"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR6228098.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5717..7179
                     /gene="citT"
                     /locus_tag="OO464_003038"
                     /pseudo
     CDS             5717..7179
                     /gene="citT"
                     /locus_tag="OO464_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7293..8099
                     /gene="rna"
                     /locus_tag="OO464_003039"
     CDS             7293..8099
                     /gene="rna"
                     /locus_tag="OO464_003039"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6228099.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8328..8738
                     /gene="rnk"
                     /locus_tag="OO464_003040"
     CDS             8328..8738
                     /gene="rnk"
                     /locus_tag="OO464_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6228100.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8969..10207)
                     /locus_tag="OO464_003041"
     CDS             complement(8969..10207)
                     /locus_tag="OO464_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR6228101.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10428..10856
                     /gene="uspG"
                     /locus_tag="OO464_003042"
     CDS             10428..10856
                     /gene="uspG"
                     /locus_tag="OO464_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6228102.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10977..12542)
                     /gene="ahpF"
                     /locus_tag="OO464_003043"
     CDS             complement(10977..12542)
                     /gene="ahpF"
                     /locus_tag="OO464_003043"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6228103.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12787..13350)
                     /gene="ahpC"
                     /locus_tag="OO464_003044"
     CDS             complement(12787..13350)
                     /gene="ahpC"
                     /locus_tag="OO464_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6228104.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13722..14468
                     /gene="dsbG"
                     /locus_tag="OO464_003045"
     CDS             13722..14468
                     /gene="dsbG"
                     /locus_tag="OO464_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR6228105.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14678..15580
                     /locus_tag="OO464_003046"
     CDS             14678..15580
                     /locus_tag="OO464_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6228106.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15727..15928
                     /locus_tag="OO464_003047"
                     /pseudo
     CDS             15727..15928
                     /locus_tag="OO464_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3951 a         4120 c         3854 g         4003 t
ORIGIN      
        1 cgcagcggaa atgccagtct ttggaaaaat gcaaactgtt tattctcgtt aagctgcaac
       61 attgttttcc ttgcgggttg tcccgagcct ctttgacgga tgtaattaaa atgttgaatc
      121 aaaatacata gatattaatt atgatcgtgg caaattttat tcattaaatc aattaaatca
      181 cttaaacctg cgagtcattt cgtaattaca ttaattaaaa aacttaaaac cattaaataa
      241 ataaaaaacc actaactcta tgtgaaataa atcaaaattt cacgccgaaa tactccttag
      301 gatgtatagc aaaaggagaa aaagatatac ctcgatcacc cccctttctc ccaagtgaaa
      361 ataaaaggtt atcagtttgc aacattgaac aacattcgtt gcaaatcgat aacaacatgc
      421 accttcagga tactatttat tatgttcggc aatgatattt tcacccgcgt aaaacgttca
      481 gaaaataaaa aaatggcgga aatcgcccaa ttcctgcatg aaaatgattt gagcgttgac
      541 accacagtcg aagtatttat taccgtaacc cgcgatgaaa agcttatcgc gtgcggtgga
      601 attgccggaa atattattaa atgcgttgct atcagtgaat ccgttcgcgg tgaaggactg
      661 gcgctgacat tagccactga actgataaac ctcgcctatg agcggcacag cacgcatctg
      721 tttatttata ccaaaaccga atacgaggcg ctgttccgcc agtgcggttt ttccacgctg
      781 acctgcgtac ccggcgtgat ggtgctgatg gaaaacagcg ccacgcgact gaaacgctat
      841 gccgaatcgc tgaaaaaatt tcgtcatcca gggaacaaga ttggctgcat tgtgatgaac
      901 gccaatccct ttacgaatgg tcaccgttat ctgattcaac aagctgcggc acagtgcgac
      961 tggttgcatc tgtttttagt caaagaagat tcttcacgct tcccctatga agaccggctg
     1021 gatctggtgt taaaaggcac cgccgatatt ccacgcctga ctgtgcatcg cggctcagaa
     1081 tacatcatct cccgcgctac gttcccttgc tacttcatta aagaacagag cgtcattaac
     1141 cattgttaca ccgaaattga tctgaagatt ttccgtcagt acctcgctcc cgcgcttggt
     1201 gtaactcacc gctttgtcgg tactgaaccc ttttgtcgcg ttaccgccca gtacaaccag
     1261 gatatgcgct actggctgga aacgccgact atctccgcag cgcccatcga actggttgaa
     1321 attgagcggc tgcgttacca ggagatgccg atatccgctt cccgggtacg tcaactgctg
     1381 gcgaaaaacg atctcacggc tatcgcgccg ctggtccctg cagtcacgct gcattatttg
     1441 cagaacctgc ttgagcactc ccgccaggac gcggcagctc gtcaaaagcc ccccgcatga
     1501 gaaacaggtg aaaaatgaaa ataaaccagc ccgccgttgc aggcaccctt gagtctgggg
     1561 atgtgatgat acgcatcgcc ccactcgata cgcaggatat cgacctgcaa atcaatagca
     1621 gcgttgagaa acagtttggc gatgcaattc gcaccaccat tctggacgtt ctcgcccgct
     1681 acaacgtgcg cggcgtacag ctgaatgtcg atgacaaagg cgcactggac tgcattttac
     1741 atgcacgact ggaagccctg ctggcacgcg ccagcggtat cccggctctg ccatgggagg
     1801 attgccaatg atttccgctt cgctgcaaca acgtaaaact cgcacccgcc gcagcatgtt
     1861 gtttgtgcct ggtgccaatg ccgcgatggt cagcaactcc ttcatctacc cggctgatgc
     1921 cctgatgttt gacctcgaag actccgtggc attacgtgaa aaagacaccg cccgccgcat
     1981 ggtttatcac gcgctgcaac atccgctgta tcgcgatatt gaaaccattg tgcgtgtcaa
     2041 cgcgctggat tccgaatggg gtgttaacga cctggaagcc gtcgttcgcg gtggtgcgga
     2101 cgttgtgcgt ctgccgaaaa ccgataccgc tcaggatgtt ctggatattg aaaaagagat
     2161 cctgcgtata gaaaaagcct gtggtcgtga acccggcagc accggcctgc tggcggcgat
     2221 tgaatctccg ctgggcatta cccgcgcagt ggaaatcgct cacgcttccg agcgtttgat
     2281 cggtatcgcc ctcggtgcag aagactatgt gcgcaacctg cgtacagaac gctccccgga
     2341 aggaactgaa ctgctgttcg cacgctgttc cattttgcag gccgcgcgct ctgcgggtat
     2401 tcaggcgttc gataccgtct attccgacgc taacaacgaa gccggattcc tgcaagaagc
     2461 cgcccacatc aaacagctgg gctttgacgg caaatcgctg atcaacccgc gtcagattga
     2521 tctgctgcac aacctctacg caccgaccca gaaagaagtg gatcacgccc accgcgtcgt
     2581 agaagccgct gaagccgccg ctcgcgaagg cctcggcgtg gtttccctga acggcaagat
     2641 ggtggacggt ccggttatcg atcgcgcccg tctggtgctc tcccgtgcag aactttccgg
     2701 catccgcgaa gaataaggca atcaaaatga cgcagaaaat tgaacaatct caacgacaag
     2761 aacgggtagc ggcctggaat cgtcgcactg aatgcgatct tgccgctttc cagaactcac
     2821 caaagcaaac ctaccaggct gaaaaagcgc gcgatcgcaa actgtgcgcc aacctggaag
     2881 aagcgattcg tcgctcaggt ttacaggacg gcatgacggt ttccttccat cacgctttcc
     2941 gtggcggtga cctgaccgtc aatatggtga tggacgtcat cgcgaagatg ggctttaaaa
     3001 acctgaccct ggcgtccagc tccctgagtg attgccatgc gccgctggta gaacacattc
     3061 gccagggcgt ggttacccgc atttatacct ctggcctacg cggtccactg gcggaagaga
     3121 tctcccgtgg tctgctggcg gaaccggtgc agatccactc tcacggcggt cgtgtgcatc
     3181 tggtacagag cggcgaactg aatatcgacg tggctttcct cggcgtcccg tcctgtgatg
     3241 aattcggtaa tgccaacggc tacaccggta aagcctgctg cggctccctc ggctatgcaa
     3301 tggttgatgc cgacaacgca aaacaggtcg tgatgcttac cgaagaactg ctgccttatc
     3361 cgcataatcc ggcaagcatt gagcaagatc aggttgattt gatcgtcaaa gttaatcgcg
     3421 ttggcgatgc tgcaaaaatc ggcgctggta ctacccgtat gaccactaac ccgcgcgaac
     3481 tgcttattgc ccgtagcgct gcggatgtga ttgtcaactc tggctacttc aaagaaggtt
     3541 tctccatgca aaccggcacc ggcggcgctt cgctggcggt aacccgtttc ctggaagaca
     3601 aaatgcgtag ccgcgatatt cgcgccgact tcgccctcgg cggtattacc gcgacgatgg
     3661 ttgatctgca cgaaaaaggt ctgatccgca aactgctgga tgtgcagagc tttgacagcc
     3721 atgctgcgca atcgctggcc cgtaacccca atcacatcga aatcagcgcc aaccagtacg
     3781 ctaactgggg ttcgaaaggc gcatcggttg atcgtctcga cgtggtggta ctgagcgcgc
     3841 tggaaattga caggcagttc aacgttaacg tgctgaccgg ttctgacggc gtactgcgtg
     3901 gcgcttccgg tggtcactgc gataccgcga ttgcctctgc gctttccatc atcgtcgcgc
     3961 cactggtacg cggtcgtatt ccgactctgg tggataacgt gctgacctgc atcaccccgg
     4021 gctccagtgt cgatattctg gtcacagacc acggtatcgc agttaacccg gcacgtccgg
     4081 aactagcaga acgtctgcag gaagcgggca ttaaagtggt ttccattgag tggctgcgcg
     4141 aacgtgcgcg tctgctgacc ggtgaaccac agccgattga attcaccgac cgcgtcgttg
     4201 ccgttgtgcg ttaccgcgat ggctcggtga tcgatgttgt gcatcaggtg aaggaataag
     4261 ccatgcacct gcttcctgaa ctcgccagcc accatgcggt gtcaattccc gagctgctcg
     4321 tcagccggga tgaaaggcaa gcacggcaac acgcctggct caagcaccat cctgttccac
     4381 tggtctcctt taccgtggtt gcgcctgggc cgattaaaga cagcgaggtc acacgccgaa
     4441 tttttaatca tcgcgtgaca gccttgcgtg ccttagccac aaagcagggc tggcaaattc
     4501 aggagcaggc tgcactggtt tccgccagcg ggccggaggg catgttgagc attgccgccc
     4561 cggctcgcga cctcaagctc gccaccattg agcttgaaca tagtcatcct ctcgggcggt
     4621 tatgggatat cgatgtcctg acgcccgaag gcgaaattct ctcccgccgc gactattcac
     4681 tgcgcctcgc cgctgcctgt tgtgcgagca aagcgcagcc gtctgcgcgc gtggaaaaac
     4741 ccatcaactg accgatttac tcaaccgcat ggaggcactg ctgaacgatg tcgatgcctg
     4801 caacgtcaac taaaaccaca aagcttgcga cgtcattaat cgatgagtac gccctgctgg
     4861 gctggcgcgc catgctgact gaagtcaatc tgtcaccgaa accaggcctc gtggatcgca
     4921 ttaactgcgg tgcgcacaaa gatatggcgc tggaagattt ccaccgcagc gcgctggcga
     4981 ttcagggctg gttaccccgt ttcattgaat ttggtgcctg tagtgcagaa atggcaccgg
     5041 aagcggtact ccacggatta cgcccaattg gtatggcttg cgaaggtgat atgttccgcg
     5101 ccactgcggg cgtaaacacg cataaaggca gcattttttc tttagggctg ctatgtgcgg
     5161 caattggccg tttgcttcag ctcaaccagc cggttacgcc aacaaccgtt tgttctacgg
     5221 cggcaagttt ctgccgtggc ctgaccgatc gcgaactgcg taccaataat tcacgactga
     5281 cggcaggtca acggttgtac cagcagcttg gccttaccgg cgcacgcggt gaagccgaag
     5341 caggttatcc actggtgatc aaccacgcct tgccgcatta cctcactctg ctggatcagg
     5401 ggttagatcc tgaactggca ttgctcgata ccttgctcct actgatggct accaacggcg
     5461 ataccaacgt tgcatcgcgc ggtggcgagg ggggcctgcg ctggctacag cgcgaggcgc
     5521 aaacattatt gcaaaaaggg ggcattcgaa cccccaccga tctcgattat ctccggcagt
     5581 tcgacaggga gtgtatcgaa cgaaatctca gtccaggcgg cagtgccgac ctgctgatcc
     5641 ttacctgttt tttagcacag atttaattat ttaagcactt gataaatttg gaaatattaa
     5701 ttttcggaga acccgtatgt ctttagcaaa agataatata tggaaactat tggccccact
     5761 ggtggtgatg ggtgtcatgt ttcttatccc tgtccccgac ggtatgccgc cgcaggcatg
     5821 gcattacttc gctgtgtttg tggcaatgat tgtcggcatg atcctcgagc caattccggc
     5881 aacagcgatc agttttattg cggttactat ttgcgttatt ggcagtaatt acctgctctt
     5941 tgatgccaaa gaattagctg acccagcgtt taatgcgcaa aaacaggcgc tgaaatgggg
     6001 cctggctggt ttttccagca ccacggtatg gctggtattt ggcgcattta tttttgcatt
     6061 agggtatgaa gtttccgggt taggtcgtcg cattgccctt ttcctggtga aattcatgga
     6121 caaacgcacg ctgacgttgg gttatgcgat tgtcattatc gacattctgc tggcaccgtt
     6181 tacaccgtcc aacaccgcgc gtaccggggg tacggttttt ccggtcatta aaaacctgcc
     6241 gccgttgttt aaatcattcc cgaacgatcc gtccgcgcgt cgtattggcg gctatttgat
     6301 gtggatgatg gtcattagta ccagtctgag ttcgtccatg tttgtcaccg gtgcggacca
     6361 aacgtgctgg gtctggagtt cgtcagcaaa attgccggta tccagattag ctggttgcag
     6421 tggttcctct gcttcctgcc ggttggggtt atcttgctta tcattgcgcc gtggctttcc
     6481 tacgtgctgt acaaaccgga aatcacacac agtgaagaag tggcaacctg ggcgggtgat
     6541 gaactaaaaa ccatgggtgc gctgacacgc agagagtgga cgctgattgg ccttgtattg
     6601 ctcagcttag gtttgtgggt atttggcagt gaagtcatta atgctactgc ggttggtctg
     6661 ctggcagttt cgctaatgct ggctctgcac gttgtgccgt ggaaagacat tacccgctat
     6721 aacagcgcat ggaacacgct ggtcaacctg gcaactctgg ttgtgatggc taacggcctg
     6781 actcgttctg gttttattga ctggttcgcc ggtaccatga gtacgcacct ggaaggattc
     6841 tcaccaaacg caacggtgat tgtactggtt ctggtgttct actttgcaca ctacctgttt
     6901 gccagcctgt ctgcgcacac cgcaaccatg ctgccggtta ttctggccgt cggtaaaggt
     6961 attccgggcg taccaatgga acaactgtgt atcctgctgg tgctgtctat cggtatcatg
     7021 ggctgtctga cgccgtatgc aaccggtcct ggggtgatta tttacggctg tggctatgtg
     7081 aaatcaaaag attactggcg tcttggcgca atcttcgggg tgatttacat ctctatgttg
     7141 ctgttggttg gctggccgat tctcgccatg tggaactaat acgttcgcca aacgctgaat
     7201 aaatcatcac gcccgccagg taagcccacc tggcgggctt ttttatgatt taatagatag
     7261 tattcagtgt atgtatgagt tccacaccca ttatgaaagc attctgccgt aacgccgcgt
     7321 tgctcgcggt ttctctgctt cccttatctt ctgccaacgc cgtagcgttg caggcaaaac
     7381 agtatggcga ttttgatcgc tatgtcctgg ccctctcctg gcaaaccgga ttttgccaga
     7441 gtcaacacga tcgaaatcgt aacgaacgag atgaatgtcg cctgcaaacc gaaacgacca
     7501 acaaaactga ttttctgacc gtacatggtc tgtggccagg attgcctaaa tcggttgctg
     7561 cccgtggtgt tgatgaacgc cgctggatgc gcttcggttg cgctactcgc ccaatcccga
     7621 atctaccaga agcacgcgcc agccgaatgt gttcatcgcc ggaaaccggg ttatcactgg
     7681 aaacggccgc taaactaagt gaagtcatgc caggagctgg cggacgttcc tgcctggaac
     7741 gctacgaata tgccaaacac ggtgcctgct ttggttttga tccggacgca tacttcggta
     7801 cgatggtacg cctgaatcaa gaaattaaag agagcgaagc cggaaaattc cttgcggata
     7861 attacggtaa aacagtgagc cgccgtgact ttgacgccgc ctttgccaaa agctggggaa
     7921 aagagaacgt gaaagcagtt aagctaacgt gccagggtaa ccctgcgtat ttgactgaaa
     7981 ttcagatctc gatcaaagct gacgccatca acgctccgct ttctgcaaac tcattcttgc
     8041 cacaacctca cccaggtaac tgtggcaaaa cctttgtgat tgataaagcg ggttattaat
     8101 cctctgattt acaaaacacg ctaattcgtc ttccataacc atgccgatta agaattatac
     8161 ataatgcgct gcattgagga attccctgaa tgtgacgcaa atcacttcaa agagtgagca
     8221 acagtcagta tcatcatgtg agttaaaccc tcgccgcctg acggtgaggg ttttcttttg
     8281 ggtatgttta tctgcgacac tcgcagtacc gacgacatgg agtaaaaatg tccagaccaa
     8341 ctatcatcat taacgacctg gatgccgaac gcatcgatat tctgctggag caacccgcct
     8401 atgctggttt gccaatcgcc gacgcgttaa acgcagagtt ggatcgcgcc caaatgtgtt
     8461 cgccagaaga gatgccacac gacgtggtga caatgaacag ccgggttaaa ttccgcaatc
     8521 ttagcgatgg cgaagtgcgt gtgcgcacgc tggtgtatcc ggcaaaaatg accgatagca
     8581 atactcagct ttccgttatg gctccggtag gtgccgcact gctggggctg cgcgttggcg
     8641 attccattca ctgggaactt ccgggcggcg ttgcaaccca ccttgaagtg ctggaactcg
     8701 aataccagcc agaagctgct ggcgactacc tgctttaatc ccatctgcac aagcgataac
     8761 tcgctttgcc cagaggatgc actcgcatcc tctttacgca aaatcagcaa tttttcctgg
     8821 ttctcactgt tcacttttgc tgcaaaatta ttccgtttcg tttgtgggaa taaccatggc
     8881 agaagcattt tacatcctga taggatttct gattatggcg gcgattatcg tcatggctgt
     8941 cctttacctc gaaaaccact cctgacgatc atattgttcc ccccggcatc gcattcacca
     9001 gacctgaaac cgccttctgc gccgcctctg cgctttgtgc accgggtacc agaatcacct
     9061 tacggcactc ctcttcacgt ttttcgaaaa tctcatatcc ccgggcggcc tcttcaaacg
     9121 gcatatagtg ggtaacaatt tcttctggtt tcagtaatcc tttctcaatt aacggtaata
     9181 attctcccag ccatgcgtga acgtgggtct gtcccatttt aaacgtcaac cctttatcaa
     9241 aggcgtcgcc aaacaggaaa ccgtgaataa atccagcgta gacgcccggt acgctaacaa
     9301 tgccgccacg cctgaccgcc gcaatacact ggcgcaacgc tttaccgctg ctgccctcca
     9361 gtttcaggtt agtcagcacc gtttccgtgg tgctgccttt cgcttcaaaa ccgacggcgt
     9421 ctattactgc atccacgccc cggtgacctg ccgtttgttc aataattgac tgtgccggat
     9481 cgctatcttc atcgaaatta atcgggatcg cgccgtagcg gtcagcggcg aaacgcaagc
     9541 ggtagggatg atgatcaaca acaaaaatct gttccgcacc gagcaaccgt gcacaggcga
     9601 ttgtcaacaa tcccacagga ccagcaccat agactgcaac gcttgaacct tgttggatct
     9661 gcacattttt tgctgcctgc catgccgttg gcagaatatc agaaaggaaa agggctttat
     9721 catctgaaag caaaggcggt actttaaacg gccccacatt ccctttcggg acgcggacat
     9781 attccgcctg cccgccagga acgccaccat acaggtgact ataaccaaac aatgccgcgg
     9841 gagcaggtat ctgtttttta ttgagcgcag cgcctttacc cgcattggta ttttcgcagg
     9901 cggcatattg ctgtaatcga cagaaaaaac agtcgccaca agcaatgacg aacggaatta
     9961 ccacccggtc gcctttttgc aaatttttta cgtccttccc ggtttcaacg acttccccca
    10021 taaattcatg accaaaaata tcgccatgtt taacctgggg tattttgcct cgataaagat
    10081 ggaggtcaga gccacagatc gccgttaccg taatacgcag aataatatca tctgcctgtt
    10141 caatgcccgg atcgggaaca ttttctacct gaacgtgatg tgggccgtga taagtcaatg
    10201 ctttcatgca acctccagag acagttcaaa aaagagtcag tctttaaggg tagctatcac
    10261 agcgggaaaa cgggcggtac cgcaggtttt gggagaattc ctagtgttca gtgagaatat
    10321 attaatcaca gaaaccgttc gtaaggaaaa agaaccagac aaatatcagg gataaatatt
    10381 ataatcatgg agaatgattt gttcatgatt aacagggaga aagggttatg tataaggcaa
    10441 tcattatgcc agttgatgta tttgaaatgg aattgagcga caaagctgtt cgccacgctg
    10501 aattcctcgc ccaggatgac ggagttattc atctacttca cgtactaccc gggtcagcca
    10561 gcctgagcct gcaccgtttt gccgctgatg tgcgtcgttt tgaagagcat ctgcaacatg
    10621 aagcagaaga acgtctgcaa acgatggtca gccacttcac catcgatcct tcccgcatta
    10681 aacaacatgt ccgttttggt agcgtgcggg atgaagtcaa tgagttggca gaagaactgg
    10741 gggctgatgt tgtagttatc ggttctcgca acccatcgat ttcgacccat ctgttaggtt
    10801 ctaacgcctc gagcgtgatc cgccacgcca atctgcctgt actggtcgtt cgctaacaga
    10861 taaaagtatt tatgccgccc tgcctggaac atcccggcag ggctttttaa tgccataaaa
    10921 aagccgccag gtttgacccc ggcggctaag caattgcagg tgaatcttac ttcttcttat
    10981 gcagttttgg tgcgaatcag gtagtcaaaa gcactcagag aggctttggc accttcgcca
    11041 gtggcgatga tgatctgctt gtacggaacc gtcgtacagt cacccgctgc gaacacgcct
    11101 ttgacgttgg tttcacattt cgcatcaatg ataatctcgc ccatgcggtt acgttcgact
    11161 gcgccttcca gccagttggt gttcggcagc agaccaatct ggacaaaaat accggccagt
    11221 tcgatgttgt gaatattgcc gctgacacga tctcgatatt ccagaccaac gactttgctg
    11281 ccgtcgcctt tcacttccgt ggtttgcgca ttcagaataa tgtcgacgtt tttcaggctg
    11341 cgcagtttgt cctgcagaac ctggtcggct ttcatttctg gcgcaaattc cagcagcgtt
    11401 acgtgctcaa cgatacccgc caggtcgatt gccgcttcca cgccggagtt accgccgccg
    11461 ataaccgcta cgcgtttgcc tttaaacagc gggccgtcgc agtgcgggca gtaggtcacg
    11521 cctttagtgc gatactgatc ttcgcccgga acgttcatgt tgcgccattt tgcacccgtc
    11581 gccacgataa tgctgcgtgc tttcagtacc gcgccagaag ctgtttcaat ctggtgtaga
    11641 ccaccttcaa ccgctgccgg gatcagtttg ctggcgctct ggctgtcgat cacatcaacg
    11701 tcgtattcat caacgtgaac tttcagtgcg cctgccaact tctgcccttc agtcttcggt
    11761 acagaaatgt agttttcgat atcaacggta tcgaggatct gaccaccaaa acgttcgccc
    11821 atcagaccgg tacggatgcc tttacgtgcg gagtaaattg ctgccgctgc acccgccggg
    11881 ccggaaccaa cgattaatac gtcataagca tcacgcttgt tcagctcttc tgccgcacga
    11941 ttttccgcgc cagtatcaat tttggcaacg atttcagtca acgtcatgcg gccctgacca
    12001 aactctttcc cgtttacgaa cactgccgga acgcccatca cgttgcgatc ggtgatttcg
    12061 ttctggaagg tgccgccgtc aattgcagtg tgcttgatgc gcgggttcaa tacgctcatc
    12121 aggttcagcg cctgcaccac gtccgggcag ttatggcaag agagcgagta ataggtttcg
    12181 aattcaaaat caccgtcaat atggcgaatc tgctccagca gagactgcgc ttctttcgac
    12241 ggatgaccac cggtccacag caacgccagt accagcgagg tgaactcgtg gcccagcggg
    12301 gagcctgcaa aacgtggtcc ctggttggaa cctgggttgg tgatcaggaa agacggctta
    12361 cgcactggca agctgttatc ttctttaaag gtgactttgt ctgacagttc tgcgatttca
    12421 gccaacagtt ccttgatttc tgccgattta gcgctgtcat ccagcgtggc aattaactca
    12481 acaggcttgg tcaatttctc aaggtaagcc ttgagttgag ttttcatatt tgtgtcgagc
    12541 atgtttatct cctgggcttt aaacatcatc atgcaagcgg cctctgcggc tacctggatg
    12601 cagcttgcat catggtgcgg ggaagaaaat cgggtgccgc agcacccgaa gaattaggtg
    12661 aatttccgaa gtatttcgcg tcagagcaag gcgactagcc tgtgaatccc cgggagctta
    12721 cacaagtaag tgaccggggt gagcgggcga cgccaacgcc gctatggcgt gaaagacgaa
    12781 ggaaatttag attttaccaa ccaggtccag agacggagcc agagttgctt caccttcttt
    12841 ccatttagcc gggcaaactt cacctgggtg agaagctacg tactgagctg ctttgatttt
    12901 acgcagcaga tcagacgcgt cacggccaat gccttcagcg gtaacttcga ttgcctggat
    12961 gataccctgc gggtcaacaa cgaaggtcgc acggtcagcc agaccttcat cttcacgcat
    13021 gttgtcgaag ttacgggtca gggcgccagt cgggtcgccg atcatcgcat atttgatttt
    13081 agcgatggtt tcagagctgc tgtgccatgc tttgtgggtg aagtgagtat cggtagatac
    13141 tgcgtatacg tctacgccca gtttctgcag ttcttcgtag tggtcagcaa cgtcacccag
    13201 ttcggtcggg catacgaaag taaagtcagc cgggtagaag aagaagacgc tccagcggcc
    13261 ttcggtatct ttttcggtga tttcgatgaa ttcgccgttt ttgaatgcct ggtttttaaa
    13321 aggtttaatt ttggtgttaa tcaaggacat ctatacttcc tccgtgtttt cgatgagatg
    13381 taaggtaacc aatttttgcc gattcggcta atgcgtttcc attatcaaat cgataagttt
    13441 taccttacaa ccttcgtaag acaactttgt gaactttgca ccgccagcgg cgataaaaag
    13501 taaaatggcc ccctttccag gtgaccgtgt aatctgaact accctttggt atcttcagcg
    13561 cctgcctgca ggctgaggtg ttgcccttac aaatgcaaca acgacatgga ttacaacacc
    13621 ctcataaaca aagggcaatc acctgatcta agctcttacc tatgacagtg ataggttatg
    13681 ccttttactc gacttttgca ctgactgaaa agaacaaatt aatgttaaaa aagatacttt
    13741 tactggctct gcttcctgca atcgccttcg cagaggaact tccttcccca gtaaaagcga
    13801 ttgaaaaaca gggcattaca atcatcaaaa cgttcgatgc ccccggagga atgaaaggtt
    13861 atctcggaaa gtatcaggat atgggcgtca ccatctacct gactcccgat ggtaagcacg
    13921 ctatctctgg ttacatgtac aacgagaaag gtgaaaacct gagtaacaca cttatcgaaa
    13981 aagaaattta cgcaccagcc ggacgcgaaa tgtggcaacg gatggaacaa tcccactggc
    14041 tcctcgacgg taaaaaagat gcgccggtca ttgtctacgt cttctccgat ccgttctgcc
    14101 catattgtaa acagttctgg cagcaggcgc gcccgtgggt agattctggc aaagtgcaat
    14161 taagaacatt gttggttggg gttatcaagc cagaaagccc ggcgacagca gcggcaattc
    14221 ttgcctccaa agatcccgca aaaacctggc aacaatatga agcctctggt tgcaagctta
    14281 agctaaacgt gcctgcaaac gtaagtacag agcaaatgaa agtgttaagt gacaatgaga
    14341 aactgatgga cgatctgggg gcaaatgtca cgccggctat ctattacatg agtaaggaaa
    14401 atacgctgca acaggccgtg gggttgcccg atcagaaaac gcttaatatc attatgggaa
    14461 ataaataaaa ggtaattcat ggatggttga actatatccc tgcttttcaa ccatcctaaa
    14521 aaagcatgaa tttctcgctc taccctaagt ggctgtgcta ttttagaaaa acttaatcct
    14581 gagttaagca aaaaaacata atcaataaaa atacatacgc aaaaaacatt gattaagtga
    14641 atataccatg gaagaaaaat ataaaccgga gtagtgtatg gccaatctct acgacttgaa
    14701 aaagttcgac ttaaatcttc ttgtcatatt tgagtgtatt taccagcatt taagcattag
    14761 caaagctgct gaatcactat atatcacccc atcagccgtg agccagtcgt tacaacgctt
    14821 acgtgcgcaa tttaatgacc ctttattcat tcgctcagga aaaggtatcg cgccgacgac
    14881 aacagggttg aatctgcatc accatcttga gaaaaacctc aaaggccttg aacaaacaat
    14941 taatatagtc aataagtccg aactaaagaa aaactttatt atctacggcc ctcagctcat
    15001 ttcttgctct aataatagta tgctgatccg ctgcctacgg caagatgcct ctgtcgagat
    15061 tgagtgccat gatatcctca tgtcagcaga gaatgctgaa gaactgttgg ttcaccgcaa
    15121 agccgatctg gtgatcaccc aaatgccagt gatcagccgt tccgtcattt gcatgccatt
    15181 gcatacaatt cgcaatactc taatttgtag taataagcat ccgcgaatta ctgacaatag
    15241 cacttatgaa caaattatgg cagaagagtt cacccaatta atagccaaat cagcaggagt
    15301 tgatgatatt caaatggaaa tagacgagaa gtttatgaat cgcaaaattt catttcgtgg
    15361 ttcctctttg ctgacaataa ttaatagcat tgcagttaca gatttactgg gaatagtccc
    15421 ctatgaactt tataactacc accgtgactt tcttaattta aaagagataa aactagaaca
    15481 tccgttacct tcaataaagc tttacatttc ctataataaa tcttctttaa ataatttggt
    15541 gttctcccga tttattgacc gtctaaatga gagtttttga tatataacta tagacagcta
    15601 ctataacttt catctattta ttcacagcgc gaatgaaaaa taaaacactc tgctttacag
    15661 tgtttacttt ataattaaca gtcttatctc actgttcacc ctgtaacgtt cagagagcgt
    15721 cttcagatgt ctatttataa aattcctctt ccgctcaata ttctggaagc tgcacgagag
    15781 cgtattacct agacactcaa caccctaccc cgcgtttgtg tctcattttc tggcggcaaa
    15841 gactctggtt taatgctgca tctgacagcc gaacttgccc gacaaatggg caaaaaaatc
    15901 tgcgttttgt ttatcaactg ggaggcgc
//
LOCUS       DAJTUI010000087        15517 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTUI010000087 DAJTUI010000000
VERSION     DAJTUI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.087"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(71..1282)
                     /locus_tag="OO464_003048"
     CDS             complement(71..1282)
                     /locus_tag="OO464_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6228107.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     FEVVNKNWPPS"
     gene            complement(1481..1565)
                     /locus_tag="OO464_003049"
     tRNA            complement(1481..1565)
                     /locus_tag="OO464_003049"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1529..1531),aa:Leu,seq:caa)
     gene            1761..2780
                     /gene="ahr"
                     /locus_tag="OO464_003050"
     CDS             1761..2780
                     /gene="ahr"
                     /locus_tag="OO464_003050"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6228108.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2909..4411
                     /locus_tag="OO464_003051"
     CDS             2909..4411
                     /locus_tag="OO464_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6228109.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4530..5612)
                     /gene="lptG"
                     /locus_tag="OO464_003052"
     CDS             complement(4530..5612)
                     /gene="lptG"
                     /locus_tag="OO464_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6228110.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5612..6712)
                     /gene="lptF"
                     /locus_tag="OO464_003053"
     CDS             complement(5612..6712)
                     /gene="lptF"
                     /locus_tag="OO464_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6228111.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6979..8490
                     /gene="pepA"
                     /locus_tag="OO464_003054"
     CDS             6979..8490
                     /gene="pepA"
                     /locus_tag="OO464_003054"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6228112.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8624..9067
                     /gene="holC"
                     /locus_tag="OO464_003055"
     CDS             8624..9067
                     /gene="holC"
                     /locus_tag="OO464_003055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6228113.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9067..11922
                     /gene="valS"
                     /locus_tag="OO464_003056"
     CDS             9067..11922
                     /gene="valS"
                     /locus_tag="OO464_003056"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6228114.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11976..13172)
                     /locus_tag="OO464_003057"
     CDS             complement(11976..13172)
                     /locus_tag="OO464_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR6228115.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13365..13868
                     /locus_tag="OO464_003058"
     CDS             13365..13868
                     /locus_tag="OO464_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6228116.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13914..14330)
                     /gene="rraB"
                     /locus_tag="OO464_003059"
     CDS             complement(13914..14330)
                     /gene="rraB"
                     /locus_tag="OO464_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6228117.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14492..15505
                     /gene="argF"
                     /locus_tag="OO464_003060"
     CDS             14492..15505
                     /gene="argF"
                     /locus_tag="OO464_003060"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR6228118.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         4012 a         3959 c         3990 g         3556 t
ORIGIN      
        1 gcactaagac cgcacgaata taacggtggg ttacccccaa acgaatcgga aaatcgatac
       61 tggaaaaact ctaactcggt ggccagtttt tgttgaccac ttcaaacatc aacctacgct
      121 ccccaagatg ttccgcctta tggatgtaag ctgctcgcac agagctacgc tcctggtgac
      181 tcatctgccg ctctaccgca tccctcgacc ataatcccga ctcaatcaac gaactacaag
      241 ccatcgtcct gaagccatgc ccacaaacct cgaccttcgt gtcatacccc attacgcgca
      301 aagccttgtt caccgtattc tcactcatcg gcttacgtgg atcgtgatcg cccacaaaaa
      361 tcagctctcg atttccgctc atgcttttga tcttttccaa aatacttagg gcctgccgcg
      421 acaaaggtac aaggtgtggt gtccgcatct tcgagccacg ctgagaatgc ttgactcctt
      481 ccagttgctc acgctcacct gggattgtcc acatagccgt ttcaaaatct atttctgacc
      541 agcgggcaaa acgcagctcg ctcgaacgga tgaaaaccaa taaagtgagt tcaaccgcca
      601 gtcgagttaa cggtctgcca gaatagtgat ctatgcgatg aagtaattca ggtatacggt
      661 tcagttcaag agctgcacgg tgctgtcttt tcgccgtagc aaccgcacct gcaatctctt
      721 gcgcggggtt gtagtcgatt aagccgctct gaacggcaaa gcgcataatc gcggtagtac
      781 gctgctgtaa acgggcggca acttcgagcc gcccggatga ctcgactgct ttaatgggta
      841 caagaagatc ccgcgtcttc agttccgcaa tgttccgctt accgatagca gtaaagagat
      901 tatcctccag acttttcaaa acacgagcgc tatgcgatgc cgaccacttc tgattgctgg
      961 cgtgccagtc tctggcgact acttcaaacg ttatcgcctc ttgttcctgt tctaccttaa
     1021 cagctttctt gttttcactg ggatcgacac cattagctaa aagcttacga gcctcatccc
     1081 ggcgtgccct ggcatccgcc aacgacactt cggggtattt ccccaatgcc agcatcttct
     1141 ctttgccgcc aaagcgataa cgtagccgcc agtatttgga gccgttaggg tgaaccagca
     1201 aaaccatgcc ttcaccgtcg gtcagcttat aggcttttgc ttcaggctta gccgaacgaa
     1261 ccttcacatc actcagagcc atgatgagta tcctttcaag ggttctgtgt gggtacaaac
     1321 attatcgaac cgggaaatac ccgcagttgt acccgcatca gtaagttgat gtagattgaa
     1381 tcaggttgac ttaggttgag tgaaaaagcg aggaaagcct tgcggatact ggattttagg
     1441 cacaaaaaaa gacgtccgtt gacgtctatt gatgttccga tggtgccgaa ggccggactc
     1501 gaaccggcac gtatttctac ggttgatttt gaatcaactg cgtctaccga tttcgccact
     1561 tcggcactga agaggtatgc ggaaaacgtt gtggattata cctgtcgcac gccaccatgc
     1621 aagcgccagc ttgcacccac ctcgctaagt gctgaaattt tcagcattac actcagcgat
     1681 atgtaacctt tgtcacactc caggcacccc gccctgccat gctctacact tcccaaacaa
     1741 caccagagaa ggccccaaaa atgtcgatga taaaaagcta tgccgcaaaa gaagcgggcg
     1801 gcgaactgga agtttatgag tacgatcccg gtgagctgaa gccacaagat gttgaagtgc
     1861 aggtggatta ctgcgggatt tgtcattccg atctgtcgat gatcgataac gaatggggat
     1921 tttcacaata tccgctggtt gccgggcatg aggtgattgg gcgcgtagtg gcactcggga
     1981 gcgccgcgca ggataaaggt ttgcaggtcg gtcagcgtgt cgggattggc tggacggcgc
     2041 gtagctgtgg tcactgcgac gcctgtatta gcggtaatca gatcaactgc gagcaaggtg
     2101 cggtgccgac aattatgaat cgcggtggct ttgccgagaa gttgcgtgcg gactggcaat
     2161 gggtgattcc actgccagaa aatattgata tcgagtccgc cgggccgctg ttgtgcggcg
     2221 gtatcacggt ctttaaacca ctgttgatgc accatatcac tgctaccagc cgcgttgggg
     2281 taattggtat tggcgggctg gggcatatcg ctataaaact tctgcacgca atgggatgcg
     2341 aggtgacggc ctttagttct aatccggcga aagagcagga agtactggcg atgggtgccg
     2401 ataaagtggt gaatagccgc gatccgcagg cactgaaagc cctgtcgggg cagtttgatc
     2461 tcattatcaa tactgtgaac gtcagcctcg actggcagcc ttattttgag gcgctgacct
     2521 atggcggtaa tttccatacg gtcggtgcgg ttctcacgcc gctgtctgtt ccggccttta
     2581 cgttaattgc gggcgaccgc agcatctctg gttctgctac cggcacgcct tatgagctgc
     2641 gaaagctgat gcgctttgcc gcccgcagca aggttgcgcc gacaaccgaa ctgttcccga
     2701 tgtcgaaaat taacgacgcc attcagcatg tacgcgatgg taaagcgcgc taccgagtag
     2761 tcctgaaagc cgacttctga tccctttgcc gggtgctgtc gcccggcact taaatctctt
     2821 tggaaggcgt ctcgcaatct gctgaattta gcggtgaaga gtgcacagcc gttgcctata
     2881 ctccaggcag gaaactggag gaaatctcat gagcgaaccc ctgttaattg cccgcacgcc
     2941 ggacacagaa ctgtttttac tgccgggaat ggctaaccgt cacgggctga ttactggcgc
     3001 aacggggacg ggtaaaaccg ttacgctgca aaaactggca gagtcattgt cggaaatcgg
     3061 cgtgccggtg tttatggctg atgtgaaagg cgatctgacc ggtatcgcgc aggcaggaac
     3121 ggcgtcggaa aaactgctca caaggcttaa aaatatcggc gtcaatgact ggcaaccgca
     3181 tgccaatccg gtggtggtgt gggatatctt tggcgagaaa ggccacacgg tgcgggcgac
     3241 ggtttcggat ctggggccgc tgttgctggc gcgactgttg aatctcaacg atgtgcaatc
     3301 tggcgtgctg aatattatct tccgcattgc ggacgatcag ggattgttgc tgctcgactt
     3361 taaagatttg cgggcgatta cccagtacat cggcgataac gccaaatcct tccagaatca
     3421 gtacggaaat atcagtagcg catcggttgg tgccatccag cgcggattac tgtcgctgga
     3481 acagcaaggc gcagcacact tctttggtga gccgatgctg gatatcaaag actggatgcg
     3541 caccgatgcc aacggtaaag gcgttatcaa tatcctcagc gccgagaaac tttatcagat
     3601 gccgaaactg tacgccgcca gcctgctgtg gatgctttca gagttgtatg aacaattgcc
     3661 ggaagcgggc gatctggaga aaccaaaact ggtgtttttc ttcgacgagg cacatctgct
     3721 gtttaacgat gcaccgcagg tactgctgga taagattgag caggtgataa ggcttattcg
     3781 ctcaaaaggc gtgggcgtct ggttcgtttc gcaaaacccg tctgatattc cggataatgt
     3841 gttcggccag ctcggtaatc gtgttcagca cgctttgcgg gcttttacgc caaaagatca
     3901 gaaagcagtg aagacagcgg cgcaaaccat gcgggccaat ccggcatttg ataccgaaaa
     3961 ggcaatccag gaactgggga ccggcgaggc gttaatctcg tttctcgatg caaaaggaag
     4021 tccttctgtg gtggaacggg cgatggtgat cgcaccttgt tcgcgaatgg ggccggtgac
     4081 ggaagatgag cgtaatggcc tgattaatca ctccccggtg tatggcaaat atgaagatga
     4141 ggtggaccgc gagtccgcct atgagatgct gcaaaaaggc tttcaggcca gtaccgagca
     4201 gcaaaataat ccccccgtga aagggaaaga ggtggcggtg gatgacggta ttcttggtgg
     4261 attgaaggat attttgtttg gcactaccgg accacgcggc gggaagaaag atggtgtggt
     4321 gcaaacaatg gcgaaaagcg ccgctcgcca ggtgacgaat cagattgtac gcgggatgtt
     4381 ggggagtttg ctggggggga gaaaaagata agtcagacgt gatctgtgcc cctcacccta
     4441 accctctccc caaaggggcg aggggaccgt tcgaactcgt atttgacttt gtcaacaata
     4501 ataaagggag ctttcgctcc cttgttagat tacgattttc tcattaacag ccacaggctg
     4561 attaagaaga agctggcact tggcaacagt gcgccgatga tcggcgggat gccgtaaacc
     4621 aacgtcagcg ggccgaagat ctggtccagt acgtagaaga caaaaccgaa gctgatacct
     4681 gtgaccacac gcacgcccat cggtacgcta cgcagtgggc caaagataaa cgacagcgcc
     4741 atcagcatca tcaccgccac ggatagcggc tggaagattt tgctccacat gttgagctga
     4801 taacgtccgg catcctgacc gctcgacttc agatacttca cgtagttgtg caaaccactg
     4861 atggagagtg catccgggtc cagcgccacc acgcccagtt tgtctggcgt gaggtcggtt
     4921 ttccaggtgc cgctcaccgt ctgcgaaccg gtaatctgtt tcggattggt cagatcagat
     4981 tcatcaacct gcgacagacg ccagacttta tgttccgggt caaacttcgc ggtagcggca
     5041 taacgtacgg attgcagacg acgattctcg ttaaaggcat aaatactgat gccacctaac
     5101 acctcgtcac ctttaacccg ctcaatgtag acgaagttgt tgccatcttt cgcccataaa
     5161 ccttgctggg tagagagcaa cgagccgccg tacatcgcct gcgcacggta gttacgcgcc
     5221 atctgttcgc cctgcggcgc gacccattcg ccaatcgcca tcgtcagcaa gaccagtgga
     5281 atggcggttt tcatcaccga cagcgctacc tgcatacggg taaaaccaga agcctgcatc
     5341 accaccagtt cgctgcgctg cgccagcatc ccaagaccaa gcaacgcccc aagaagagcc
     5401 gccatcggga agaagatctg cacatctttc ggcacgctca gcaaggtata cattcctgcg
     5461 cctaacgcgt cgtaactccc ctgcccggct tttttcagct gatcgacaaa tttgataatg
     5521 cccgacagcg acaccagcat gaacagcgtc atcatgatgg tggtgaaaat agttttaccg
     5581 atatagcggt caagtacgcc aaaaggttgc atcacaccgc tcctttacgc gaaaaactgg
     5641 cgcgcaggcg gcggaccggc acggtgtccc aaaggttgag aacaatcgcc aaagccagat
     5701 aaatcaggtt aacggtccac atccacagtg tcgggtccag cttaccttta ccgccgttcg
     5761 atttcaggga ggtctggatc aggaagaaaa gtagatacag cagcatggct ggcagcatcg
     5821 acagtacgcg tccctgacgc gggttaacca cgctcaacgg tacaaccata agtgccatca
     5881 taaacacggt gaataccaac gtgatacgcc agttcagttc tgcgcgagca cgatcggtgt
     5941 cagtgttcca caatgtgcgc atgtccatct ggtcggtatc gttcgggtcg agcgccaccg
     6001 cctggtgacc aatgatcgcc tgataatcct ggaaatccgt aatgcggaaa tcacgtaaca
     6061 gtgcagtgcc ttcgaagcgc gttccctggt tgagagtgac gacctgggag ccgtcgcgca
     6121 gctgggttaa atgtccggaa tcggccacca ccacagaagg acgtgcatta ccttttggtc
     6181 gaatttgtgc gaggaacaca tctttgaaat cgctgccgtc aacgctttcg atgaacagca
     6241 ccgagctgcc attagtcgct tgctggaatt gcccttgcgc cagcgccgcc atgccagggt
     6301 tcgctttcgc ttctgctaac acttcatcct gatgacgcga tgaccacggt cccgcccaca
     6361 tcacgttaac cgccgcgacg attgccgtga ataccgcaag gatcattgcc gctttcacca
     6421 gaaccgcttt gctcaagccg caggcatgca ttaccgtaat ttcactttcg gtatacagtt
     6481 tgcccagcgt catcagcagc ccgaggaaca ggcttaatgg caggataagc tgcgccattt
     6541 ccggcacgcc caacccgaga agggagagca ccagattcgc cggaatatcg ccgtcaaccg
     6601 ctgcgccgag gatcctcact aacttttgac agaagaagat caaaagcaag atgaagagta
     6661 tcgccagctg gcttttgagc gtctcccgca ccagatatct tatgattatc actttaaata
     6721 cgcccgtaaa aactcgtctt ttgcaggatt ttagcttgtt tcatggctta aacgtcattt
     6781 attctcttga gtcgtcgaaa tcgtcgctaa gataattata ctcaacggat tcacctctca
     6841 gattttgttc tgacgtgcca atgccgtaat aacgttaaga ttaacacgaa gtcatcgcaa
     6901 cagcggacat gagttacgaa agcttgcaat tctatctgta gccaccgccg ttgtctttaa
     6961 gattcaggag cgtagtgcat ggagtttagt gtaaaaagcg gtagcccgga gaaacagcgg
     7021 agtgcctgca tcgtcgtggg cgtcttcgaa ccacgtcgcc tttctccgat tgcagaacag
     7081 ctcgataaaa tcagcgatgg gtacatcagc gccctgctac gccggggcga actggaagga
     7141 aaaccggggc agacattgtt gctgcaccat gttccgaatg tactttccga gcgaattctc
     7201 cttattggtt gcggcaaaga acgtgagctg gatgagcgtc agtacaagca ggttattcag
     7261 aaaaccatta atacgctgaa tgatactggc tcgatggaag cggtctgctt tctgactgaa
     7321 ctgcacgtta aaggccgtaa caactactgg aaagtgcgtc aggctgtcga gacagcaaaa
     7381 gagacgctct acagtttcga tcagttgaaa acgaataaga gcgaaccgcg tcgtccgctg
     7441 cgtaaaatgg tgttcaacgt gccgacccgc cgtgaactga ccagcggtga gcgcgcgatc
     7501 cagcacggtc aggcgattgc cgccgggata aaagcagcga aagatctcgg taatatgccg
     7561 ccgaatatct gtaacgccgc ttatctcgcc tctcaggcgc gccagctggc tgacagctac
     7621 agcaagaatg tcatcacccg cgttatcggc gaacagcaga tgaaagagct gggaatgcac
     7681 tcttatctgg cggtcggtca gggttcacaa aacgaatcgc tgatgtcggt gattgagtac
     7741 aaaggcaacg cgtcggaaga tgcacgccca atcgtgctgg tgggtaaagg tttaaccttc
     7801 gactccggcg gtatctccat caagccttca gaaggcatgg atgagatgaa gtacgatatg
     7861 tgcggcgcgg cggcagttta cggcgtgatg cgtatggtcg cggagctgca actgccgatt
     7921 aacgttattg gcgtgctggc aggctgtgaa aacatgcctg gcgggcgtgc ctatcgtccg
     7981 ggcgatgtgt taaccaccat gtccggtcaa accgttgaag tgctgaacac cgatgccgaa
     8041 ggccgcctgg tactgtgcga cgtgttaact tacgttgaac gttttgagcc ggaagcagtg
     8101 attgacgtgg cgacgctgac cggtgcctgc gtgatcgcgc tgggtcatca tattactggt
     8161 ctgatgacga accataatcc gctggcccat gaactgattg ccgcgtctga acaatccggt
     8221 gaccgcgcat ggcgcttacc gctgggtgac gagtatcagg aacagctgga gtccaatttt
     8281 gccgatatgg cgaacattgg cggtcgtcct ggtggggcga ttaccgcagg ttgcttcctg
     8341 tcacgcttta cccgtaagta taactgggcg cacctggata ttgcaggaac cgcctggcgt
     8401 tctggtaaag caaaaggcgc aaccggtcgt ccggtagcgt tgctggcaca gttcctgtta
     8461 aaccgcgctg ggtttaacgg cgaagagtaa gtttgcgttt taccctcacc ctaaccctct
     8521 ccctaaggga gagggaaccg atcgagcgca atagaaattc attgctcatc tttcagggtg
     8581 aggaatgcag caatatttaa atccaccaca agaagcccca tatatgaaaa acgcaacgtt
     8641 ctaccttctg gacaatgaca ccaccgtcga tggcttaagc gccgttgagc aactggtgtg
     8701 tgaaattgcc gcagaacgtt ggcgcagcgg taagcgcgta ctcatcgcct gtgaagatga
     8761 aaagcaggct taccggctgg atgaagccct gtgggcgcgt ccggcagaaa gctttgttcc
     8821 gcataattta gcgggagaag gaccgcgcgg cagtgcgccg gtggagatcg cctggccgca
     8881 aaaacgcagc agcagcccgc gcgatatatt gattagtctg cgaacaagct ttgcagattt
     8941 tgccaccgct ttcacagaag tggtagactt cgttccttat gaagattctc tgaaacaact
     9001 ggcgcgcgaa cgctataaag cctaccgcgt ggctggtttc aacctgaata cggcaacctg
     9061 gaaataatgg aaaagacata caacccacaa gatatcgaac agccgcttta cgagcactgg
     9121 gaaaagcagg gctactttaa gcctaatggc gatgaaagcc aggaaagttt ctgcatcatg
     9181 atcccgccgc cgaacgtcac cggcagtttg catatgggtc acgccttcca gcaaaccatc
     9241 atggatacca tgatccgcta tcagcgcatg cagggcaaaa acaccctgtg gcaggtcggt
     9301 actgaccacg ccgggatcgc tacccagatg gtcgttgagc gcaagattgc cgcagaagaa
     9361 ggtaaaaccc gtcacgacta cggccgcgaa gctttcatcg acaaaatctg ggaatggaaa
     9421 gcggaatctg gcggcaccat tacccgtcag atgcgccgtc tcggcaactc cgtcgactgg
     9481 gagcgtgaac gcttcaccat ggacgaaggc ctgtccaatg cggtgaaaga agttttcgtt
     9541 cgtctgtata aagaagacct gatttaccgt ggcaagcgcc tggtaaactg ggacccgaaa
     9601 ctgcgtaccg ctatttctga cctggaagtg gaaaaccgcg aatcgaaagg ttcgatgtgg
     9661 cacatccgct atccgctggc tgacggcgcg aaaaccgcag acggtaaaga ttatctggtg
     9721 gttgcgacca ctcgcccgga aaccctgctg ggcgataccg gtgtagctgt taacccggaa
     9781 gatccgcgtt acaaagatct gattggcaaa tatgtcattc tgccgctggt taaccgtcgt
     9841 attccgatcg ttggcgacga acacgccgac atggaaaaag gcaccggctg cgtgaagatc
     9901 acaccggcgc acgactttaa cgactatgaa gtcggtaaac gtcacgccct gccgatgatc
     9961 aacatcctga cctttgacgg tgatatccgt gaaagcgccc aggtgttcga taccaaaggt
    10021 aacgaatctg acgtttattc cagcgagatc ccggcagagt tccagaaact cgagcgtttt
    10081 gctgcacgta aagcagtcgt tgctgcggtt gatgcactcg gcctgctgga agaaattaaa
    10141 ccgcacgacc tgaccgttcc ttacggcgac cgtggcggcg tggttatcga accgatgctg
    10201 accgaccagt ggtacgtgcg tgccgatgta ctggcgaaac cggcagttga agcggttgag
    10261 aacggcgaca ttcagttcgt accgaagcag tacgaaaaca tgtacttctc ctggatgcgc
    10321 gatattcagg actggtgtat ctctcgtcag ctgtggtggg gtcaccgtat cccggcatgg
    10381 tatgacgaag cgggtaatgt ttatgttggc cgcaacgaag aagaagtgcg caaggaaaat
    10441 aacctcggtg ccaacgttgc cctgcgtcag gacgaagacg ttctcgacac ctggttctct
    10501 tctgcactgt ggaccttctc tacccttggc tggccggaaa ataccgacgc cctgcgtcag
    10561 ttccacccaa ccagcgtgat ggtatctggt ttcgacatca tcttcttctg gattgcccgc
    10621 atgatcatga tgaccatgca cttcatcaaa gatgaaaatg gcaaaccgca ggtgccgttc
    10681 cacactgttt acatgaccgg tctgattcgt gacgacgaag gtcagaagat gtccaaatcc
    10741 aagggtaacg ttattgaccc gctggatatg gttgacggta tttcgctgcc agaactgctg
    10801 gaaaaacgca ccggcaatat gatgcagccg cagctggcgg acaaaatccg taagcgcacc
    10861 gagaaacagt tcccgaacgg cattgagccg cacggcaccg atgccctgcg cttcaccctg
    10921 gcggcgctgg cgtctaccgg tcgtgacatc aactgggata tgaagcgtct ggaaggctac
    10981 cgtaacttct gtaacaagct gtggaacgcc agccgctttg tgctgatgaa cacagaaggt
    11041 caggattgcg gcttcaacgg cggcgaaatg acgctatcgc tggcggaccg ctggattctg
    11101 gcggagttca accagactat caaagcgtac cgcgaagcgc tggacagctt ccgcttcgat
    11161 atcgccgcag gcattttgta tgagttcacc tggaaccagt tctgcgactg gtatctggag
    11221 ctgaccaagc cggtaatgaa cggtggaaca gaagccgaac tgcgcggtac tcgccatacg
    11281 ctggtgactg tactggaagg tctgctgcgc ctcgcgcatc cgatcattcc gttcatcacc
    11341 gaaaccatct ggcagcgtgt gaaagtactt tgcggtatca ctgccgacac catcatgctg
    11401 cagccgttcc cgcagtatga tgcatctcag gttgatgaag ccgcactggc cgacaccgag
    11461 tggctgaaac aggcgatcgt tgctgtacgt aacatccgtg cagaaatgaa catcgcgccg
    11521 ggcaaaccgc tggagctgct gctgcgtggt tgcagcgcgg atgcagaacg tcgcgtaaat
    11581 gaaaaccgtg gcttcctgca aacgctggcg cgtctggaaa gcatcaccgt gctgcctgcc
    11641 gatgacaaag gtccggtttc cgttaccaag atcatcgacg gcgcagagct gctgatcccg
    11701 atggctggcc tcatcaacaa agaagatgag ctggcgcgcc tggcgaaaga agtggcgaag
    11761 atcgaaggtg aaatcagccg catcgagaac aagctggcaa atgaaggctt tgtcgcccgc
    11821 gcaccggaag cggttatcgc gaaagagcgt gagaagctgg aaggttatgc agaagcgaaa
    11881 gccaagctga ttgaacagca ggctgttatc gccgcactgt aatcaacaca gtgatgaaaa
    11941 cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa agaaggcata
    12001 actccccgag aaatactggc taagaagcct ttatctggtt gttcttcttt attgattaaa
    12061 ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc aatcatccat
    12121 attttcagca gtgggtaagc cagaccaccc gtaatcccgg atatcaccac caacgcacac
    12181 atgcgataaa gcataccgtg gtacgttaaa gttgagcgaa aacgaatacg cccatcattc
    12241 agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact ggtgctaaca
    12301 gcaatcccaa aataataaat taactgcgcg attatcattt tacgttgcat ttccataaat
    12361 tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc atttaatatt
    12421 tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagctaa aaatgcgagg
    12481 atggcatatt ttatacaata cgcagtatct aatttcacct tgaaacgatg tataccgaaa
    12541 ctggtattgc tccagagaaa actcattacc aggctatata aagtaccatt aaaaatacca
    12601 atggaaacaa taccaactat tgccatcaga accacagata taataacact acttgaacta
    12661 ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaat ggccattaaa
    12721 atcggcaaga aaaaggttac ccaccagaac cctttcaaag agcaattaaa actaaatctt
    12781 acgccgttga gactggtcat taaggcctga taacgcagtc cttttgctgc cataaatata
    12841 agcaaaacca acagtaacaa agtcaaaaca cagccaacaa gcggcatatc tgctgacact
    12901 gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa aacattccca
    12961 ccggtaattc cataagaaaa tcgttgtccg ttaacttcca tattagcata aagataacgc
    13021 ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt taacaacaca
    13081 ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa agaatgggac
    13141 ggaacatcca tttcattaat aacttgagcc ataaaaatcc cctgaattaa aaaatccatt
    13201 gatataattt atagatacag acagtaaaat gttctgccac gttacaatac tcttatcagt
    13261 aataggctgg caaaaacttt attgctttat aagacgcttg cgcacgtaag aagagagtgg
    13321 tattaaacaa atgtataatt actttatttt tattgggtta aactatgaat aacattgcgc
    13381 cgcaatcacc tgtaatgcgt cgcctgacgc tgcaggataa tcctgctatc gccagcgtca
    13441 tccgtcaggt ttacgcagag tatggtctta ccgccgataa aggctacacc gttgccgatc
    13501 cgaatctcga cgaactgtat caagtgtaca gccagcctgg ccatgcttat tgggtggttg
    13561 aatacaatgg cgaagtggtc ggcggcggcg ggattgcgcc attaacaggt agtgaagcgg
    13621 acatttgcga actgcagaaa atgtactttc ttcccgctat tcgcggtaaa gggctagcga
    13681 aaaaactggc cttaatggcg atggagcagg cacgagagat gggcttcaaa cgctgctatc
    13741 tggaaacgac cgctttttta aaggaagcca ttgcgcttta tgagcatttg ggctttgagc
    13801 atatcgacta tgcgcttggc tgcacgggcc atgtcgattg tgaagtgcgg atgctacgcg
    13861 aactctaaaa aaatgcccgc tagtatgtgc tatcgggcat tttaatcaat taattagtgg
    13921 cgaaccccgt catcgtcttc atcgacaaaa tcttcatcgt cgccatcttc gccgttagga
    13981 tcttcaaagt aagtgcccca accgtcgtac tcaacgtcaa atttctccgc cagcgtcatc
    14041 agttgttcaa cctgggcatc gatcagatcg gcattcaacg cgcactcgct gaggatgtcg
    14101 cagcaaatca cgatatcacc atcttcaact tccagctctt ctggatcggt cacttcgtaa
    14161 ccgagtttaa acgcttcaac tgccgctttt tccagagttt ccagatcgtc tgcggaaaga
    14221 tggtgttcga tggtgtacag cgcgtccgga tcgctgccat cttccagtaa ttcttcaata
    14281 atcaaacgtg tttcttcacg ctgttcttcc agttgttccg ggtttgccat ggctcattcc
    14341 tcttaatgtc ctgccgatac tcttattgtc acacaccact gacattgcct ccacctttgt
    14401 gacaaagatt tatgctttag acttgcaaat gaataataat acacataaat tgagttttaa
    14461 ttcattgagg cgttagccac aggagggatc tatgtccggg ttttatcata agcatttcct
    14521 gaaattactc gatttcacgc cagctgaact caacagcctg ctgcagttag ccgcgaagct
    14581 gaaagctgat aagaaaagcg gtaaagaaga agccaaactc actggtaaaa acatcgcgct
    14641 catcttcgaa aaagactcga ctcgtacccg atgctctttc gaagttgccg catatgacca
    14701 gggggctcgc gttacttatc ttggcccaag cggtagtcag attggtcata aagagtcgat
    14761 taaagacact gcccgcgtgc ttggtcgcat gtatgacggt attcagtatc gcggctatgg
    14821 tcaggagatt gtcgaaacac tggcggaata cgctggcgtg ccggtatgga acggcctgac
    14881 caatgagttt caccccacgc agttgctggc ggatctcctc accatgcagg agcatttacc
    14941 cggtaaagca ttcaacgaaa tgacgctggt ctatgcaggt gacgcacgta acaacatggg
    15001 caattcgatg ctcgaagctg cggcgcttac cggtctggat ttgcgtctgg tcgcgccaca
    15061 ggcatgctgg ccagaagctg cgctggttac ggaatgccgc gccctggcac aacaaaatgg
    15121 cgggaatatt acgctgacgg aagatgtagc taaaggagtt gaaggtgctg actttatcta
    15181 taccgatgta tgggtgtcga tgggtgaagc aaaagaaaaa tgggctgaac gcattgcatt
    15241 actgcgtgat tatcaggtga acagcaagat gatgcagttg accggtaacc cggaggtcaa
    15301 attcctccat tgcctgcccg cgtttcatga cgaccaaacg acgcttggca aaaaaatggc
    15361 ggaagagttt ggcctacatg gcggaatgga agtgactgat gaggtcttcg aatctgccgc
    15421 cagcattgta tttgatcagg cggaaaaccg catgcatacc atcaaagcgg tgatggtcgc
    15481 gacgctcagt aaattaaata attaatatct ctataaa
//
LOCUS       DAJTUI010000088        15342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTUI010000088 DAJTUI010000000
VERSION     DAJTUI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.088"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OO464_003061"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OO464_003061"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6228119.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OO464_003062"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OO464_003062"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6228120.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OO464_003063"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OO464_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR6228121.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="OO464_003064"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="OO464_003064"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6228122.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="OO464_003065"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="OO464_003065"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6228123.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="OO464_003066"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="OO464_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6228124.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="OO464_003067"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="OO464_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR6228125.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="OO464_003068"
     CDS             complement(8064..9206)
                     /locus_tag="OO464_003068"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR6228126.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..10228)
                     /locus_tag="OO464_003069"
     CDS             complement(9215..10228)
                     /locus_tag="OO464_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR6228127.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OO464_003070"
     CDS             complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OO464_003070"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6228128.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OO464_003071"
                     /gene_synonym="rfaI"
     CDS             complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OO464_003071"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6228129.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHIFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OO464_003072"
     CDS             complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OO464_003072"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6228130.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12826..13950)
                     /locus_tag="OO464_003073"
     CDS             complement(12826..13950)
                     /locus_tag="OO464_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6228131.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="OO464_003074"
     CDS             complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="OO464_003074"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6228132.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4379 a         3444 c         3145 g         4374 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttttttcttc aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt
     9541 tagcatgatg tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta
     9601 taattgactc atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt
     9661 cccgtaaatt aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac
     9721 acaaacgctc agcgctttta ctttgcatag catctacatc aattaccact gccccatatt
     9781 catctttaaa ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat
     9841 caagatataa taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat
     9901 aaatagatac tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat
     9961 gatataattt aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact
    10021 ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa
    10081 gaattgaagc aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga
    10141 cattaaacgt ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt
    10201 taaattgagt aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc
    10261 tcttcaatcc tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata
    10321 taagatatgt atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat
    10381 caatgcgatc ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc
    10441 ttaaaccttt ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat
    10501 gctgatgcaa ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca
    10561 aatactcatt taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga
    10621 aatttaatgt tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac
    10681 cacgtacccg atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt
    10741 ttaagaaacg ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac
    10801 gagcagtgtc aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt
    10861 taaccctgca agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta
    10921 tgaaaacttt caacccattt atcgttttat tatatatcat aatgaattat tttaacctta
    10981 aatctttaga agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa
    11041 tgcttttgtt taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta
    11101 agtaaatctt ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc
    11161 ggataatcag cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg
    11221 tcatcattga ctggattatc aactttgtct tttaattcat agttaatact atatcgggta
    11281 ttatatttct ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga
    11341 tcaaggtggg tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac
    11401 gaaatgttat ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat
    11461 ccagaagcta actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct
    11521 ctttcagcta caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta
    11581 atactaccct tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag
    11641 aaataatctg caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt
    11701 aatgacttca gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt
    11761 gctaaatcag aaaaatataa tttgtcttta tcactgatat aatctgtgaa aatatgaaat
    11821 tcacaagaga tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa
    11881 agaaaatttc tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga
    11941 taatgaaaat tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt
    12001 ctgtatccaa aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt
    12061 tccctgattt ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga
    12121 atgtctttaa gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc
    12181 aggccaatat tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct
    12241 ggaacgcgcg tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct
    12301 tccttaccgg aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta
    12361 atgcccgcag catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt
    12421 acgcgaacat ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt
    12481 ggtgtcagat cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg
    12541 ccgaatgcca ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc
    12601 cattcgcggt ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct
    12661 ttcagggtcg tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc
    12721 agagtacggc gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca
    12781 aaaggatctt tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc
    12841 acccgttatg atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata
    12901 atgtcgcgca ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa
    12961 aatctcattc aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc
    13021 gacaatataa tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat
    13081 cgcttccagc agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc
    13141 cgccgccatt aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac
    13201 gccgcgtttt tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag
    13261 caatgtgttg tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc
    13321 acctttacgc gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc
    13381 attcttctta cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg
    13441 ataaatcccc ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa
    13501 atcggcgatt tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt
    13561 tgcccgctca aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt
    13621 ttcctgcgct actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg
    13681 cattttgttg aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca
    13741 ggcaaaatac tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac
    13801 ttcaaataca tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc
    13861 tcgggcggcg actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa
    13921 atatttatat aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt
    13981 aatttatcga cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt
    14041 cggtcacgct gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg
    14101 ttatttgacc agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc
    14161 gtattaactg cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca
    14221 tgatcgatta acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt
    14281 ggcgtctggc atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg
    14341 gacgtcagaa cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg
    14401 gcgttgtccc agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt
    14461 ccaacaccag cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc
    14521 atggttgtct gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt
    14581 tccaccacat ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca
    14641 gactgccgat gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt
    14701 atagcaacca tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat
    14761 accttgatga gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt
    14821 atgccgtaga gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa
    14881 agcagcacat cgatttttgc gtcagggtaa ttttttttca gagaactaat gacgggagta
    14941 gttaataaca tatccccatg aaaacgcatc ttaatgagca aaattcttcg aaatggctta
    15001 tccacaagta actctttttg ttgagattat ccgattaagg tgaagcagaa aaagcacgct
    15061 accgccccag gctcaacagc tacctgaata ctgataccgc actgatattg acgcgctagt
    15121 gtatcacttc tttcgcggca atccgacccg aatgattttt aagcacattg ttgattttca
    15181 ataaagaaat caatcaaata aaatcattag caaccatatt ctttcactgt tgctctgcga
    15241 ttccttcctg cacgtacagc agaaatcaat gaatcgcata aaaatacaaa aaaagtatag
    15301 atgttcaaca agaattggcg ggtaaattgc tatttaagtc aa
//
LOCUS       DAJTUI010000089        15320 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTUI010000089 DAJTUI010000000
VERSION     DAJTUI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.089"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO464_003075"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO464_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR6228133.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO464_003076"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO464_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR6228134.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OO464_003077"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="OO464_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO464_003078"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO464_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6228135.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="OO464_003079"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="OO464_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6228136.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="OO464_003080"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="OO464_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6228137.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="OO464_003081"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="OO464_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6228138.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="OO464_003082"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="OO464_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6228139.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="OO464_003083"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="OO464_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6228140.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="OO464_003084"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="OO464_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6228141.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="OO464_003085"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="OO464_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6228142.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="OO464_003086"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="OO464_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6228143.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="OO464_003087"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="OO464_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6228144.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO464_003088"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO464_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6228145.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="OO464_003089"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="OO464_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6228146.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="OO464_003090"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="OO464_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6228147.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="OO464_003091"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="OO464_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6228148.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="OO464_003092"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="OO464_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6228149.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="OO464_003093"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="OO464_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6228150.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="OO464_003094"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="OO464_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6228151.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="OO464_003095"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="OO464_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6228152.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="OO464_003096"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="OO464_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6228153.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="OO464_003097"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="OO464_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6228154.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="OO464_003098"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="OO464_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6228155.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="OO464_003099"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="OO464_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6228156.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO464_003100"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO464_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6228157.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="OO464_003101"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="OO464_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6228158.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="OO464_003102"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="OO464_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6228159.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="OO464_003103"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="OO464_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6228160.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="OO464_003104"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="OO464_003104"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6228161.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..>15320
                     /gene="rplQ"
                     /locus_tag="OO464_003105"
     CDS             15058..>15320
                     /gene="rplQ"
                     /locus_tag="OO464_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR6228162.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFA"
BASE COUNT         3958 a         3592 c         4078 g         3692 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctaaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaagccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtt gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc
//
LOCUS       DAJTUI010000090        14468 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTUI010000090 DAJTUI010000000
VERSION     DAJTUI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.090"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(10..951)
                     /gene="fabY"
                     /locus_tag="OO464_003106"
                     /gene_synonym="yiiD"
     CDS             complement(10..951)
                     /gene="fabY"
                     /locus_tag="OO464_003106"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6228163.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(996..1433)
                     /gene="dtd"
                     /locus_tag="OO464_003107"
     CDS             complement(996..1433)
                     /gene="dtd"
                     /locus_tag="OO464_003107"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6228164.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1430..2302)
                     /locus_tag="OO464_003108"
     CDS             complement(1430..2302)
                     /locus_tag="OO464_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR6228165.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2296..2895)
                     /gene="yihX"
                     /locus_tag="OO464_003109"
     CDS             complement(2296..2895)
                     /gene="yihX"
                     /locus_tag="OO464_003109"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6228166.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(2994..3779)
                     /locus_tag="OO464_003110"
     CDS             complement(2994..3779)
                     /locus_tag="OO464_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6228167.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(3813..4709)
                     /gene="yihV"
                     /locus_tag="OO464_003111"
     CDS             complement(3813..4709)
                     /gene="yihV"
                     /locus_tag="OO464_003111"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR6228168.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            4877..5773
                     /gene="yihU"
                     /locus_tag="OO464_003112"
     CDS             4877..5773
                     /gene="yihU"
                     /locus_tag="OO464_003112"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR6228169.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            5797..6675
                     /gene="yihT"
                     /locus_tag="OO464_003113"
     CDS             5797..6675
                     /gene="yihT"
                     /locus_tag="OO464_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR6228170.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6692..7933
                     /gene="yihS"
                     /locus_tag="OO464_003114"
     CDS             6692..7933
                     /gene="yihS"
                     /locus_tag="OO464_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR6228171.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8071..8973
                     /locus_tag="OO464_003115"
     CDS             8071..8973
                     /locus_tag="OO464_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR6228172.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9172..11208
                     /gene="yihQ"
                     /locus_tag="OO464_003116"
     CDS             9172..11208
                     /gene="yihQ"
                     /locus_tag="OO464_003116"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR6228173.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11254..12628
                     /locus_tag="OO464_003117"
                     /pseudo
     CDS             11254..12628
                     /locus_tag="OO464_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12671..14074
                     /locus_tag="OO464_003118"
     CDS             12671..14074
                     /locus_tag="OO464_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6228174.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14142..>14468
                     /gene="ompL"
                     /locus_tag="OO464_003119"
     CDS             14142..>14468
                     /gene="ompL"
                     /locus_tag="OO464_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR6228175.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3491 a         3769 c         3648 g         3560 t
ORIGIN      
        1 ctccataagc tactcttctt cgttcccgcc ctcttcatac gggccaaatg gcttcgcggg
       61 cagaacgata tacgtgcctt caaacactgc acccggcgtc tcgtcgccaa agatttcgac
      121 ctgcatctgc acccgtgctt ttcgtccgcg cgccagacgg tcgagatcgc cgcttaaggc
      181 accaaggtcg gctaccgcat ggggtttacc gctaatcggt ttgctgtagc ggatatgcgc
      241 atccgccaga ataatcgttc cgccgaggtg gcgttcacgc agcatcagcc agataagccc
      301 ccaaccggtg agcgtcgcca gcgagaataa actcccggca aacagcgtat ggtgcggatt
      361 ctgattgccg gtttccggca tggtggtgat aaatttttgc ccggtatatt gttgaatacg
      421 cacgcccatt ttttcactaa gcgggatatg ttcgtaccac gcctgttgca gctgcgcgca
      481 ccagtcgccg cgatgcaaaa tatcatccag agtggcgacg ggcttaatca tcaaaaaatg
      541 gcgaatcggc gtggtggttg gcgtggtgat ttctccctga ttaacaaacc ccagcttggc
      601 gaaaaactcc accgcgtctt cacgggcgct acaggtcacg cgcttaacgc cttcctgacg
      661 cgccaccgac tccagggtca tcgccatcag cgttcctaac cctttgtcct gcacgtccgg
      721 gtgaacgacc ataaagcgaa tggacgcttc attgtcggca ttaatataca atcggcctac
      781 cgccaccaga ttaccctgct cgtcgacgac catctggtga tgcgccatcg catcccacgc
      841 gtcgcgttcc gaaccttttg gttgatgcag gggcttacgc aacatttccc agcgaaagtg
      901 atagtaacgc tctaactctt cttctgtttg tggaacctga aggtgataca tagcgatact
      961 ctctcttgtt tcccgtgaca accctggaag ctggctcata cctgcaacca gaatgtcacg
     1021 gggccatcat tgaccagcga tacctgcata tcggcagcga agcgtcctgt ttgcgtgttc
     1081 atctcttgct ggcggcagcg ttcgacgaaa tagtcatata acgcctctgc gcgatccggt
     1141 gatgcgcctt tggagaaact tgggcgcatc ccccgttcgg tatctgcggc gagggtaaac
     1201 tgggaaacca ccagtacact gccgcccgcc tgttgcacgt tgagattcat cttgccttcg
     1261 gcatcgctaa agatgcggta gccgagcaca cgctcgcaca gacggtttgc tttctgttcg
     1321 tcgtcatcct tttcgacacc caataacacc aaaagtcccg cgccaatttc gcccgtcact
     1381 tctccctcca cggtgacgct ggcacgggtt acgcgttgaa ttaatgcaat catggttcgt
     1441 cgtcttcttc ttgttcagct gcttgtttta gtttgcggta ttccccgaga gtgacagtaa
     1501 tttccgcgcc aagcaagacg atacaccatg tccagtagac ccaaacaaag agaatgggga
     1561 tcaccgccag cacaccgtaa atgagctgat atgacgggaa catggtgata taaagcgcga
     1621 aacctttctt tcctgcttcg aacaggagtg cggcgacaaa cgcgccgaca atcgcgtcgc
     1681 ggttaggtac gcggatggta ggaacaatgc tgtacagcaa ccagaaggag atccacgaca
     1741 acagcaacgg aaaaatacgc agcacgttgt cgatgacagt attgagatcg ctcgcccagc
     1801 gcagggagag caaataggaa ctgatcgcca gactggcccc tgccagcagc ggccccagcg
     1861 ttaaaatcat ccagtacacg gcgaacgagt aaatttttgg tcgcgctcgt ttactgcgcc
     1921 agatggtatt caacgcgcta tcgatggagt acatcaacaa taacgcggtg acgatcagcc
     1981 cgcacgcccc aacggcggtc atcttgttgg aattagcaac aaattgttcg atataccgct
     2041 gaataacatc gccagtagca ggcagaaagt tggcaaaaat aaagtgacgt aactgaatgc
     2101 tgacgtcgga aaacatggga aaagcggcga aaagcgcaaa aacaacggca accagcggca
     2161 ctaatgagag caacgacaca taggcaaggt tacctgccag ggttgtcatg ttgtcctcat
     2221 caatacgttg ccagagtagt tttagccagg cccatagtgg acgggtacgg tgcctggctt
     2281 tgtcctgaat ggtttttagc ataacacctt cgcgaaatag tccgggatgg tggttttatc
     2341 tttcaccaga atactggtaa tgcctagctg attagctcct tctatattat cggcgttatc
     2401 gtcgaaaaag accgtatcgc tgggtgaaaa accttccgcc tgcaaaacat gctggtaaat
     2461 tcgtgcttca ggtttgcgca tccccagatc ttgcgacaga tagatatggt cagcagcatc
     2521 acgaatttcc gggtattctt ccggccagaa ggtggtatgc aggcggttgg tattggaaag
     2581 caccaccacg cgatgcccct gctcacgcag tttatgcatg atggcgatca cttccgggcg
     2641 cagcgcaaca aacaccgcct gccagccgtg agagaactgc tcgtagctta gcggtagagc
     2701 catctcatga cacagcgcct ctgcgaacgc ttcgtcgcta atttccccac gctcatgctg
     2761 atgaaacgcc tcccccatat gaaaactctt cttaagcgat gccagcggaa tacgcgttaa
     2821 atcgctccag gctcccagca cacggttaaa gtcgatatcg acaatcacat tacctaaatc
     2881 aaagatatag agcatttttg cctcctttcg cgccacaaga aattaactgt agcgggaaag
     2941 gatgggtttg gctatgtggc aaggtatgaa gccaggttaa cggtcacaaa aatctacgcg
     3001 tcttcctggg gaagaatgat ttcaatttgg ctgtcttgta agcgtgattt tagcgcagcg
     3061 ggcggcagct cgtcggtaaa gagcgcagtg acctgtgcca cgttaccaat ttcaaccgcc
     3121 gccgaagcat gatacttagt gtgatcggcg accagcagaa tatttctcgc gtgcgccatc
     3181 atcgttttca ccacgttagc ttcgtttaca tcaaactcca tcaacgcgcc atcgctctca
     3241 atcgccccaa cgcttgttac cagataatca gcacgaaaat cagccacaaa ggacgccgct
     3301 gaagggccaa tgatcccgct attatgagag cgcaacgtac cgccgggcac catcacttca
     3361 aagcgcgggt tgtggtaaag aatatgcgcc acacgcaggc tgttggtgat tatccgcaaa
     3421 tgattatggt taagtaacgc ccgggcaaca tgctcaacag tcgtaccaat ggtgataaat
     3481 attgttgaac catcaggaat atagtctgcc accgcttcgg caatcgcttt ttttttctcg
     3541 gtttgcgaaa cctcacgctg ctcgaacgcc gtattaacga cgctggaagc ccgacccgcg
     3601 ccaccgtgat ggcgcgtaat caggccttgc tcgcttaatt tacgaatatc ccggcggacc
     3661 gtctgcgtgg agacatccag cagatttgcc agctcatcaa tattcatata cccacgctcg
     3721 gcgatcagca tgaggagttg gtcgtgccgc gggttaccgg ttagttcggt aaggctcatg
     3781 aaaattcctc gaaaaaccat caccctggca ttttatacaa aaagtgacaa aaaagatcgg
     3841 gtttgatcac agtcagggat cccggctcgt ccaccgggac gtgtgcattt taacgccgct
     3901 acaccgctgg cgaagcggac tgactccgct aaatccccac ttgttgccag cgccaccgcc
     3961 aaagcgccgt gaaaaacatc tcccgcacct gtggtatcta ccacatcaac tttgaaggcc
     4021 ggttgatgct gacgcccacc attttccagc cagtcgcagc ctgcgctacc ctgggtcaca
     4081 tagacatgac catttgtgag cgtttgtgcc tgttttagcg cactggccat ctctttcacg
     4141 cccgttaagc gcgccagacc cggttctgaa aaggccgcgt gatcgcttaa tgccaccagc
     4201 tcactgatat cctgcggcgt aatgtccccg tccagaacgg tcatcacacc cgcctgacgg
     4261 gccagggtga aggctttttt agcgccgtcg tgccagcgta catctgccag cacaacatcc
     4321 cactgagaga aatcaatttc ctccaaccac tctgcgtcag gcagcagatc cgggctgggg
     4381 tagttaatga ttatccgctc gcctttggta tccaccatga tggcggattg cgaagatttc
     4441 gcctggttat accgtttggt gtaacgggtg ttaacgcccc aggattccag ctctgccagc
     4501 aggctgttgc ccgcgtcgtc atcacctacg cgaccaataa aatcgacctg cgcccccagc
     4561 cttgccgccg caaccgctgc cgtcgccgct ggcccgccac caacttccgt ataatttctc
     4621 gccacgtatt taccgctctc cgtcggtaac ccttccacgt aatagatgcg atccatcacg
     4681 gttataccta cacaagcaac acgaatcatg gtcattcctt aaacattttg ggatgcgttc
     4741 attttaattt caccaattgt ttaaaaagtg acggctgtca attttttgac gataaattac
     4801 aaattcgatc aaaaacagac aagcaaagca tgtcataaat gacaaaaagt gacatcactg
     4861 tattcaggag agggttatgg cagcaatcgc gtttatcggt ttaggacaaa tgggttcgcc
     4921 aatggcgagc aatttattgc agcaagggca tcaacttcgc gtctttgatg tgaacgccga
     4981 agctgtgcgg catctggtag acaaaggtgc gactcccgcc gccaacccgg cgcaggccgc
     5041 taaagatgcc gaatttatca ttaccatgtt gccgaatggc gatctggtgc gcaacgtgtt
     5101 gttcggtgaa aacggcgttt gcgaaggctt atctaccgat gcgctggtca ttgatatgtc
     5161 caccatccat ccgctgcaaa ccgataaatt gattgccgat atgcaagcca aaggcttcag
     5221 catgatggat gttccggtag gccgtacttc tgcaaatgcc attaccggta ctctgttact
     5281 gctggctggc ggcaccgctg aacaagttga acgtgccaca ccgatcctga tggcgatggg
     5341 cagtgagttg atcaacgctg gcggtccggg catggggatc cgcgttaagc tcatcaacaa
     5401 ctacatgagc atcgcgctca atgcgctttc ggcagaagcc gccgttttgt gcgaagccct
     5461 gaatcttccc ttcgatgttg ccgtcaaagt gatgagcggt accgccgccg gtaaaggtca
     5521 cttcaccact tcctggccaa acaaagtcct cagcggagat ctttctcccg ccttcatgat
     5581 cgatcttgcc cataaggatc ttggcatcgc ccttgatgtc gccaaccagc tgcatgtgcc
     5641 aatgccgctg ggggccgcct cacgggaggt ttatagccag gcgcgcgcag cgggtcgcgg
     5701 tcgccaggac tggtccgcca ttctggaaca ggtccgtgtc agtgccggga tgactgccaa
     5761 agtaaaaatg taacgactgg ataaaggaat aaatgaatga ataagtacac catcaacgac
     5821 attacgcgcg catcgggcgg ttttgccatg ctggcggtcg atcagcgcga agccatgcgc
     5881 atgatgtttg ccgcggctgg ggcacccgct ccggtagccg atagcgtttt aactgatttc
     5941 aaagttaacg ctgcaaaggc cctctcgcct tatgcctcgg cgattctggt agatcaacaa
     6001 ttctgctacc gccaggtggt tgagcaaaac gcgattgcca aaagttgcgc catgattgtc
     6061 gccgccgatg agttcattcc tggcaacggt attccggtcg atagcgtggt tattgaccgc
     6121 aaaatcaatc cgctacagat caaacaagac ggtggcaaag ccttaaaact gctggtgctg
     6181 tggcgtagtg atgaagatgc gcagcaacgt ctggatatgg tgaaagagtt caacgaactg
     6241 tgccactcac acggtctggt aagcatcatt gagccagtcg tccgcccacc gcgtcgtggc
     6301 gataaattcg atcgcgaaca agcgatcatc gatgccgcca aagagctggg cgacagtggc
     6361 gctgacctct acaaagttga aatgccgctt tatggcaaag gtccgcaaca agagcttctc
     6421 tgtgcttcac aacgtctgaa tgaccatatc aatatgccat gggtgatcct ctcttccagt
     6481 gtcgacgaaa aactgttccc gcgtgccgta cgcgtggcaa tgacggcggg cgcatcggga
     6541 ttcctggcag gccgtgcagt ctgggcatcg gtcgtcggtt taccagacaa cgagctgatg
     6601 ctccgtgacg tttgcgcacc gaaattacaa caacttggcg atatcgtcga cgaaatgatg
     6661 gctaaacgcc gctaagatga ggataaaagg aatgaaatgg tttaacaccc taagccacaa
     6721 ccgttggctg gaacaggaaa ccgaccgcat ctttgatttt ggtaaaaatt ccgtagtgcc
     6781 gactggtttt ggctggttag gcaataaagg gcaaatcaaa gaagagatgg gcacccatct
     6841 gtggatcaca gctcgtatgt tgcacgttta ttccgttgct gcggcgatgg gtcgacctgg
     6901 cgcttacgcg ttggttgatc acggtattaa agccatgaac ggcgcactgc gtgataaaaa
     6961 atatggcggc tggtatgcct gcgtgaatga cgagggcgtg gtggatgcct ccaaacaggg
     7021 ctatcaacac ttctttgctc tgctgggtgc cgccagcgcc gtcacaacgg gtcacccgga
     7081 agcgcgcaag ctgctcgatt acaccattga gattatcgag aaatatttct ggagcgaaga
     7141 agagcagatg tgcctggagt cctgggacga agccttcagc aaaaccgaag agtaccgcgg
     7201 cggcaatgcc aatatgcacg cggtggaagc tttcttgatt gtttatgacg tcactcacga
     7261 caaaaaatgg ctggatcgcg cgattcgcgt ggcttccgtg attatccacg acgtcgccag
     7321 aaataatcat tatcgcgtta acgaacactt cgatacccag tggaatccgc tgccggatta
     7381 caacaaagat aacccggcgc atcgcttccg cgcgttcgga ggtacaccag gccactggat
     7441 cgaatggggc cgtttaatgc tgcacatcca tgctgccctg gaagcccgtt gcgaacaacc
     7501 accagcatgg ctgctagaag atgccaaagg tctgtttaac gccaccgtgc gcgatgcctg
     7561 ggcacccgat ggagcggacg ggattgttta taccgttgac tgggaaggaa aaccggtggt
     7621 ccgcgaacgt gtacgttggc ctatcgtcga agcaatgggt acggtctacg cgctctacac
     7681 cgtcactggc gatcgccagt atgaaacctg gtatcaaaca tggtgggagt actgcattaa
     7741 gtacctgatg gactatgaaa atggttcctg gtggcaggag ctggatgcgg acaataaggt
     7801 catcaccaaa gtctgggacg gcaaacagga tatttatcac ctgctgcatt gcctggtgat
     7861 cccgcgtatc ccgttagcgc ccggcatggc tccagcggtt gcagcgggtc tgctggatat
     7921 taatgcgaaa taactgatct gaaaataggg tgcgggtttt cccctcaccc taaccctctc
     7981 cccaaagggg cgaggggacc gtccggtgca gtttttgggt ttgacatcag cctcacggga
     8041 ggctgtatgt cgttaattaa ggtgccttgt atgcaaatca ccaatatgca ttgcagtgga
     8101 cagaccgttt cacttgccgc gggggattat cacgcgacca ttgtgaccgt cggtgctggt
     8161 ctggctgaat taacctttca gggttgtcat ttagtcatcc cgcacaaacc cgaagagatg
     8221 ccgctggcgc atctgggtaa agtgcttatt ccgtggccga accggatcgc caacggctgt
     8281 taccgttatc aggggcagga atatcagtta cccattaatg aacatagttc aaaagccgct
     8341 attcacgggc tgcttgcctg gcgagactgg caaattagtg aattaaccac caccagcgtt
     8401 accctgacag cttttctgcc gcccagttac ggctatccgt ttatgctggc ttcacaagtt
     8461 gtttattcgc tcaacgcaca cacgggtctt tccgttgaga tcgcaagcca gaatatcggc
     8521 actgtcgcag cgccttatgg cgtgggtatt catccttatt taacctgcaa cttaacatct
     8581 gttgatgaat atctgttcca gcttccggca aaccaggttt atgccgtcga tgaacacgta
     8641 aatccgacaa cgcttcacca cgttgatgag ctggatttaa atttcacgca ggcgaaaaaa
     8701 atagccgcga caaaaatcga ccacaccttt aaaaccgcta atgatttgtg ggaaatgacc
     8761 attacccatc cacagcaggc gctttcggta agcctttgtt ccgatcaacc ctgggtgcag
     8821 gtttatagcg gtgaaaaatt acagcgtcag ggactggcag ttgaacctat gagttgcccg
     8881 cctaacgcct ttaattcagg tatcgactta ttattactgg aaccaggaaa accgcaccgc
     8941 ctgtttttta atatttacgg acagagaaaa taacttgtat tgattagcag tcaaaatcat
     9001 tgaagaacat ttcactcgct aaaggcaaca ttatgtatct tcaatttatt aagttaaaaa
     9061 cagctcagct tctgtaaagt ttttatatat cagttttatg agaatcattt tacataagcc
     9121 tgtaataaca ggaaggcaac aatctcgttc tcaactctat ggagattagt tatggatacg
     9181 ccacgtccac agttattaga ttttcaattt catcagaata acgacagttt taccctacat
     9241 tttcaacaac gtcttatttt aacccataac aaagataatc cttgtttatg gattggctca
     9301 ggtatagcgg atatcgatat gttccgcggt aatttcagca ttaaagataa actacaggag
     9361 aaaattgcgc ttaccgacgc catcgtcagc cagtcaccgg atggttggtt aattcatttc
     9421 agccgtggtt ctgacattag cgccacgctg aatatctctg ccgacgatca gggccgttta
     9481 ttgctggaac tacaaaacga caaccttaac cacaaccgta tctggctgcg ccttgccgct
     9541 caaccagagg accatatcta cggctgcggc gaacagtttt cctacttcga tctgcgcggc
     9601 aaaccgttcc cgctatggac cagtgaacaa ggcgttggtc gcaacaaaca aacctatgtc
     9661 acctggcagg ccgactgcaa agaaaatgcg ggcggcgact attactggac tttcttccca
     9721 cagcctacgt ttgtcagcac gcagaagtat tactgccatg ttgataacag ttgctacatg
     9781 aacttcgact ttagcgcccc ggaataccat gaactggcgc tgtgggaaga caaagctacg
     9841 ctacgttttg aatgtgctga cacatacatc tccctgctgg aaaaattaac tgccctgctg
     9901 ggacgccagc cagaactgcc cgactggatt tatgacgggg taacgctcgg cattcagggc
     9961 gggacggaag tgtgccagaa gaaactggac accatgcgta acgcgggcgt gaaggttaac
    10021 ggcatctggg cgcaggactg gtctgggatc cgtataacct cttttggcaa gcgcgtgatg
    10081 tggaactgga agtggaacag cgaaaactac ccgcaactgg attcacgcat taagcagtgg
    10141 tatcaggagg gcgtgcagtt cctggcctat atcaacccgt atgttgccag cgataaagat
    10201 ctctgcgaag aagcggcaca acacggctat ctagcaaaag atgcctctgg cggtgactat
    10261 ctggtggagt ttggcgagtt ttacggcggc gttgtcgatc tcactaatcc agaagcctac
    10321 gcctggttca aggaagtgat caaaaagaac atgattgaac tcggctgcgg cggctggatg
    10381 gctgacttcg gcgagtatct gcccaccgac acgtacctgc ataacggcgt cagtgccgaa
    10441 attatgcata acgcctggcc tgcgctgtgg gcgaagtgta actacgaagc ccttgaagaa
    10501 acgggcaagc tcggcgagat cctcttcttt atgcgcgccg gttctaccgg tagccagaaa
    10561 tactccacca tgatgtgggc gggcgaccag aacgtggact ggagtctcga cgatggcctg
    10621 gcgtccgtag tcccggcggc gctgtcgctg gcaatgaccg gacatggtct gcatcacagc
    10681 gacattggcg gttacaccac cctgtttgag atgaagcgca gcaaagagct gctgctgcgc
    10741 tggtgcgatt tcagcgcctt cacgccgatg atgcgcaccc acgaaggtaa ccgtcctggc
    10801 gacaactggc agtttgacgg cgatgcggaa actatcgccc attttgcccg tatgactacc
    10861 gttttcacca ccctgaaacc ttatctgaaa gaggccgtcg cgctgaatgc gaagtccggt
    10921 ctgccggtca tgcgcccgct gttcctgcat tacgaagacg atgcacatac ctattccctg
    10981 aaatatcagt acctgttagg ccgcgacatt ctggtcgctc cggtacacga agaaggtcgc
    11041 agcgactgga cgctctatct gccggaggat aactgggtac atgcctggac gggtgaagcg
    11101 ttccggggcg gggaagttac cgttaatgcg cccatcggca agccgccggt cttttatcgc
    11161 gccgatagcg aatgggcggc actgttcgcg tcgttaaaca gcatctaatc acatccgccc
    11221 gtggggaaac tcatgggcaa agggagaaca tcgatgagtc acatcacaac ggaagatcca
    11281 gcaactttac gcctgccctt taaagagaaa ctctcttacg gtattggcga cctggcctct
    11341 aacatcctgc tggatatcgg tacgctttat cttttgaagt tttataccga cgttctgggg
    11401 ctgccaggca cctatggcgg cattatcttt ttgatttcaa aattctttac tgcgtttacc
    11461 gatatgggta ccggcattat gttggattcc cgacgcaaga tcggtccaaa aggtaagttc
    11521 cgtcctttta ttctgtatgc gtcattcccg gtcaccttac tggcgatcgc caactttgtc
    11581 ggcacaccgt ttgatgtcac cggtaaaacg gtgatggcca ctattctgtt tatgctttac
    11641 ggactgtttt tcagcatgat gaactgctcc tacggcgcaa tggtcccggc aatcaccaaa
    11701 aaccccaacg aacgcgcctc actggcggca tggcgtcagg gcggcgctac gctgggcctg
    11761 ctgctgtgca cggtgggatt cgtgccagtt atgaatctta tcgaaggtaa tcagaaactt
    11821 ggctatatct tcgccgccac gctgttttca ctgttcggcc tgctgtttat gtggatctgc
    11881 tactcaggcg tgaaagagcg ttatgtcgaa acccaaccaa ccaatccggc gcaaaagcct
    11941 ggcctgttgc aatctttccg cgcaattgcc ggtaaccgcc cactgttcat tctgtgtatt
    12001 gccaacctct gcaccttagg ggcgtttaac gtcaagctcg ccattcaggt ctattacacc
    12061 cagtacgtac ttaacgatcc catcctgttg tcgtatatgg gatttttcag catgggctgt
    12121 attttcatcg gcgtgttcct gatgcctggc gcagtcagac gttttggtaa gaagaaggtc
    12181 tatatcggcg gcctgctgat ttgggtgctg ggcgatctgc tcaactattt cttcggcggc
    12241 ggctcggtca gctttgtggc gttctcctgc ctggcgttct tcggctcagc gcctgaactg
    12301 ggcgctggtt tccgacaccg tcgagtacgg cgagtggcgc accggcgtgc gttcggaagg
    12361 cacggtctac accggcttca ccttctttcg caaagtgtct caggcgctgg ctggtttctt
    12421 ccccggctgg atgctgacgc aaattggcta tgtgccgaac gtcgcacagg ctgaccacac
    12481 cattgaaggg ttacgccagt tgatcttcat ctacccaagc gcactggcgg tagtcaccat
    12541 tgtggcgatg ggttgcttct acagcctgaa cgagaagatg tatgtccgca ttgtggaaga
    12601 gatagaagcc cgtaaacgca cggcgtaata ataataaacg acgccctgcg gggcgttata
    12661 aggagtgatt atgtctgacc ataatccact gacattaaaa ctgaatctgc gggaaaaaat
    12721 cgcctatggt atgggcgacg tcggttcgaa tttaatgctc tgcatcggta ctctgtatct
    12781 cctcaaactt tataccgatg aattagggat gcctgcttac tatggcggga ttatctttct
    12841 ggtcgcgaag ttttttaccg cgtttaccga tatgctcacc ggatttttac tcgactcgcg
    12901 gaaaaatatt gggcctaaag gcaaatttcg tccctttatt ttatatgcag cagttccggc
    12961 ggcgttaatt gcaacgctcc agtttatcgc caccaccttt tgtttgccgg ttaaaacgac
    13021 gattgccacc acgctgttta tgatgtttgg actttcatac agtctgatga actgctcgta
    13081 tggtgcgatg atcccggcaa ttaccaaaaa cccgaatgag cgcgcgcagc tcgcggctta
    13141 ccgccagggt ggtgcaacca tagggctatt gatttgtacc gtggcgttta ttccgttgca
    13201 gtcgcttttt tctgactcaa ccgtcggtta tgcctgtgcg gcacttatgt tctccattgg
    13261 cggctttatt tttatgatgc tgtgctacag aggcgtcaaa gagcattatg tggacacagc
    13321 gccaaccgga cataaagcca gtattctcaa atctttttgc gcgatatttc gtaatccacc
    13381 attgctggtt ttatgcattg ctaacctgtg tactctggcg gcatttaata tcaaactggc
    13441 gattcaggtc tattacaccc agtatgtgct gaatgatatt aatttattgt cgtggatggg
    13501 attcttcagt atgggatgca tcctcgtcgg cgtattactg gtgccagtaa ctgtaaaatg
    13561 ttttggtaaa aaacaggttt atttagctgg catggtgcta tgggcggtgg gtgatatact
    13621 gaattatttc tggggaagta actctttcac tttcgtcatg ttctcttgtg tcgccttttt
    13681 tggcacggcc tttgtaaaca gcctgaactg ggcactggta ccagataccg tagattacgg
    13741 tgaatggaaa accggtattc gtgccgaagg ttctgtttat accggttata ccttctttcg
    13801 taaaatttct gccgcacttg ctggcttctt gccaggcatt atgctgacgc aaattggtta
    13861 tgttcccaac atcgcgcaaa gcgatgcgac attacagggt ttgcgtcagc tcatttttat
    13921 ctggccttgt gcactggcaa ttattgctgc attgacgatg ggattctttt acacactcaa
    13981 tgaaaaacgg tttgcattga ttattgagga aatcaaccaa cgcaaaaata aagagatggc
    14041 gacagaaaaa aaaacggctt ccgtaacgtt ataaatgtca atagccgcta tttccatcct
    14101 ggtgggtggc ggcctcccta cgtttaaaaa atggacttat tatgaaaaag attaatgcaa
    14161 taattttatt atcttctctg acctctgcct cggtatttgc tggcgcatac gtcgaaaaca
    14221 gggaggcata taatcttgcc tccgatcagg gcgaggttat gctacgtgtg ggttataact
    14281 tcgatatggg cgcgggtatt atgttaacca atacctacac ctttcagcga gaagatgaac
    14341 taaaacatgg ctataatgaa attgaaggtt ggtatccttt attcaaacca accgataaat
    14401 taaccatcca gcccggtggc ttaattaatg ataagagtat cagttcgggt ggtgcagtgt
    14461 atctggac
//
LOCUS       DAJTUI010000091        14452 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTUI010000091 DAJTUI010000000
VERSION     DAJTUI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.091"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(114..380)
                     /locus_tag="OO464_003120"
     CDS             complement(114..380)
                     /locus_tag="OO464_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6228176.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            654..4178
                     /gene="nifJ"
                     /locus_tag="OO464_003121"
     CDS             654..4178
                     /gene="nifJ"
                     /locus_tag="OO464_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6228177.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4545..5674
                     /gene="ompN"
                     /locus_tag="OO464_003122"
                     /pseudo
     CDS             4545..5674
                     /gene="ompN"
                     /locus_tag="OO464_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5815..6249
                     /gene="uspF"
                     /locus_tag="OO464_003123"
     CDS             5815..6249
                     /gene="uspF"
                     /locus_tag="OO464_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6228178.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6425..7360
                     /gene="ttcA"
                     /locus_tag="OO464_003124"
     CDS             6425..7360
                     /gene="ttcA"
                     /locus_tag="OO464_003124"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6228179.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7489..8856)
                     /gene="dbpA"
                     /locus_tag="OO464_003125"
     CDS             complement(7489..8856)
                     /gene="dbpA"
                     /locus_tag="OO464_003125"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6228180.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9334..10317)
                     /gene="zntB"
                     /locus_tag="OO464_003126"
     CDS             complement(9334..10317)
                     /gene="zntB"
                     /locus_tag="OO464_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6228181.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10572..11804
                     /gene="dgcM"
                     /locus_tag="OO464_003127"
                     /pseudo
     CDS             10572..11804
                     /gene="dgcM"
                     /locus_tag="OO464_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11825..12388)
                     /gene="smrA"
                     /locus_tag="OO464_003128"
                     /pseudo
     CDS             complement(11825..12388)
                     /gene="smrA"
                     /locus_tag="OO464_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12642..14108)
                     /locus_tag="OO464_003129"
     CDS             complement(12642..14108)
                     /locus_tag="OO464_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR6228182.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGIACGLAWNIQI
                     MILFRALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLT
                     DNLDWRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYT
                     LEEGARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFS
                     FSGGVGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRW
                     LLMAGLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKER
                     LKLASGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSAL
                     FFNRSRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14105..>14452)
                     /locus_tag="OO464_003130"
     CDS             complement(14105..>14452)
                     /locus_tag="OO464_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6228183.1"
                     /translation="TGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRA
                     DILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLL
                     RPGLSVTVSVDER"
BASE COUNT         3856 a         3645 c         3406 g         3545 t
ORIGIN      
        1 aacaccatta gcgaaatgct gaaagaaggt gcacaagtgg atctgaccgc gaaccagtta
       61 acgctggcga ccgcgaaaca gacattaact tataagctgg cggatttaat gaattaatag
      121 ctgccacagc tcccggcggc aagtgactgt tcgctacagc gtttgccgtt gggtaatgca
      181 cacatcccaa tcgccgtacc atccagttga cgggcaacag aaagcgaacc gccgatcatt
      241 gcacaatttg cttctccact actggacatc gacgctttta aacttggcgc tacgtgcgcc
      301 gcagtcgcct gctgaacagg ctcactacta cacgccgaca acaataaagc ggcacaccct
      361 acccaaaacg ctgctcgcat ctcttcttcc tctgatcttc aagccaaacg acaccgccat
      421 aaataatagg cagcacagag ggcggcgtcg agagctgtcc tgcgcgttgc cccgcatttt
      481 tactttttta tggctatttt tttgccctct gtttgatcaa aacattcatt actctgatgt
      541 gggggacaca aaagcgaaaa tgcagaagaa agccatttgc taaaattgaa ggattactac
      601 tgggcgcgca gcaatttcgt gcgcccctca tttgcgcaat gtaagggtgt catatgatta
      661 ctattgacgg taatggcgcg gttgcttcgg tgacgtttcg catcagtgaa gttatcgcca
      721 tctaccctat tacccccagt tccacgatgg cagaacaggc tgatgcctgg gccggaaacg
      781 gcttgaagaa cgtttgggga gacacaccac gcgtggttga aatgcagtcg gaagcgggtg
      841 ctatcgctac cgtgcatggc gctttgcaga cgggtgccct ttcaacatcg tttacgtcat
      901 cgcagggttt gctgctgatg atcccgacgc tgtacaaact ggcaggcgaa ctgacgccat
      961 ttgtcctgca tgtagcggca cgtaccgttg ccacacatgc actttctatt tttggcgatc
     1021 attccgacgt tatggcggtg cgccagacgg gttgcgcgat gttgtgtgcg gctaacgtcc
     1081 aggaagcgca agactttgct ctcatttcgc acatcgcaac gctgaagagc cgcgtgccat
     1141 ttattcattt ctttgatggt tttcgcacgt cccacgaaat caataaaatt gtcccgctgg
     1201 ccgatgacac tattcttgat ctcatgccgc aggccgaaat tgatgctcat cgcgcccggg
     1261 cgctcaaccc ggaacatccg gtgatccgcg gtacgtccgc caatcctgac acttatttcc
     1321 agtctcgcga agccaccaat ccctggtaca acgcggtcta tgaccatgtt gaacaggcga
     1381 tgaatgattt cgctgccgcg acaggtcgtc agtatcagcc gtttgagtat tacgggcatc
     1441 cgcaagcgga acgggtgatt atcctgatgg gctctgccat tggcacctgt gaagaagtgg
     1501 ttgatgaatt gctaacccgt ggcgaaaaag ttggcgtgct gaaagttcgt ctgtaccgtc
     1561 ccttctccgc caaacacttg ctgcaagctc tgccgggatc cgtacgcagc gtggcggtac
     1621 tggacagaac caaagaaccc ggtgcccagg cagaaccgct ctatctggat gtgatgaccg
     1681 cactggcaga agcctttaat aatggtgagc gcgaaaccct gccccgtgtc attggtgggc
     1741 gctatggtct ttcatccaaa gaatttggcc cggactgtgt actggcggta tttgccgagc
     1801 tcaacgcggc taaacctaaa gcacgcttta cggttggtat ttacgatgat gtgaccaatc
     1861 tgtcactgcc gttgccggaa aacactctgc caaactcggc gaaacttgaa gccttgtttt
     1921 atggccttgg tagtgatggc agcgtttccg caaccaaaaa caatatcaag attatcggta
     1981 attccacgcc gtggtacgca cagggctatt ttgtttacga ctccaaaaaa gcgggcggcc
     2041 tgacggtttc tcaccttcgc gtgagcgaac agccgattcg ttccgcttat ctcatttccc
     2101 aggctgattt tgttggctgc caccagttgc agtttatcga taaatatcag atggctgagc
     2161 gtttaaaacc tagcggcatt ttcctgctta atacgccgta cagcgcagat gaagtgtggt
     2221 cgcgcttgcc gcaggaagtt caggccgtgt taaaccagaa aaaagcgcgc ttctatgtga
     2281 ttaacgcggc gaaaatcgcc cgcgaatgtg gcctggcggc ccgtattaat accgtcatgc
     2341 agatggcttt tttccatctg acgcaaattc tgcctggcga tagcgccctc gcagaattgc
     2401 agggtgcgat tgccaaaagt tacagtagca aaggtcagga tctggtggag cgcaactggc
     2461 aggctctggc gctggcgcgt gaatccgtag aagaagttcc gttgcaaccg gtaaatccgc
     2521 acagcgccaa tcgaccgcca gtggtttccg atgccgcccc tgatttcgtg aaaaccgtaa
     2581 ccgcggcgat gctcgccggg cttggtgatg ccctccccgt ttcggcgctg ccgccagacg
     2641 gcacctggcc gatgggcacc actcgctggg aaaaacgcaa tatcgccgaa gagatcccca
     2701 tctggaaaga ggaactctgt acccaatgta accactgcgt cgccgcttgc ccacactcag
     2761 ctattcgcgc aaaagtggta ccgcctgaag cgatggaaaa cgcccctgcc agcctgcatt
     2821 cgctagatgt gaaatcgcgt gatatgcgcg ggcagaaata tgtcttgcag gtggcaccgg
     2881 aagattgcac cggttgtaac ctgtgcgtcg aagtttgccc ggcgaaagac cgtcagaatc
     2941 cagagattaa agccatcaat atgatgtctc gccaggaaca tgtcgaagaa gagaaaatca
     3001 attacgattt cttcctcaac ctgccagaaa tcgaccgtag caaactggaa cgtattgata
     3061 ttcgtacatc gcagctgatt acaccgctgt ttgaatattc aggtgcttgc cccggttgtg
     3121 gcgagacgcc gtatattaaa ttgctgactc agctctatgg tgaccggatg ttgatcgcta
     3181 acgccaccgg ctgttcttca atttatggcg gtaacctgcc ctctacaccg tataccaccg
     3241 atgccaacgg tcgtgggccg gcatgggcga actctctatt tgaagataat gccgaatttg
     3301 gccttggttt ccgcctgacg gtcgatcaac accgtgtccg cgtgctgcgt ctgctggatc
     3361 aatttgccga taaaatcccg gcggaattac tgacggcgtt gaaatcagac gctacgccag
     3421 aggttcgtcg agaacaggtc gcagctttac gccagcaact caacgatgtt gccgaagcac
     3481 atgaacttct gcgtgacgca gatgcactgg tggaaaaatc aatctggctg attggtggcg
     3541 acggctgggc ttacgatatc ggctttggcg gtctggatca tgtattgagt ttgacggaaa
     3601 acgtcaacat tctggtgctg gatacgcaat gctattccaa caccggtggt caggcgtcga
     3661 aagcgacacc gctgggtgca gtaactaagt tcggcgagca cggcaaacgt aaagcgcgta
     3721 aagatcttgg cgtcagtatg atgatgtacg gtcatgttta tgtggcgcag atttctctcg
     3781 gcgcgcagct gaaccagacg gtgaaagcga ttcaggaagc ggaagcgtat ccgggaccat
     3841 cgctgatcat tgcttatagc ccgtgtgaag agcatggtta cgatctggct ctcagccacg
     3901 accagatgcg ccaactcact gctaccggct tctggccgct atatcgcttt gatccgcgtc
     3961 gtgccgatga aggcaaactg ccgctggcgc tggattcacg cccgccgtca gaagcactgg
     4021 aagaaacgtt acttcacgag cagcgtttcc gtcgtctgaa ttcgcagcag ccagaagtgg
     4081 cagaacagtt atggaaagat gctgcagcta atttgcaaaa acgctatgac ttcctagcac
     4141 aaatggccgg aaaagcggaa aaaagcaaca ccgattaaat gctctggata aggattatcc
     4201 aattctaaaa aaaaagcccg gacgactgtt cgggcttgtc tttttatatg ttggaaaatc
     4261 agtggcaatg caatggccca acagaaaata aaatctgtga caataaaggc atataacccg
     4321 cgcagaataa cgtataggaa attaaaagta taatttttat tttgtatgta ttatcttcga
     4381 tacattcacc agacttatta tcatttcaga ggaattattt gattaaggtt ttacttaagg
     4441 cgtaacaaat gatatttatc ggctaactga acttctcctt taggatgttt tcactccccg
     4501 aaatggggat aaaaggcaaa taaaataact aaaggattta ttcaatgaaa agaaaagtac
     4561 tggcactttt aattcctgcc ctgctcgccg caggtgctgc acatgcagcc gaagtttata
     4621 ataaagacgg caacaaatta gatctgtatg gcaaagttga tggcctgcat tatttttctg
     4681 ataattcagc gaaagatggc gaccagagct atgcgcgtct gggttttaaa ggcgaaaccc
     4741 aaattaacga tcaacttact ggctacggtc aatgggaata caatattcag gcaaacaaca
     4801 ctgaatcttc aaaaaaccag tcatggaccc gtctggcatt tgccggactg aaatttgcag
     4861 attacggttc tttcgattac ggacgtaatt atggcgtaat gtacgacatc gaaggctgga
     4921 ccgatatgct gcctgaattt ggcggtgact cttataccaa tgcagacaac tttatgactg
     4981 gtcgagccaa tggcgtcgcg acttatcgta atactgattt cttcggtctg gtaaatggtc
     5041 tgaacttcgc ggtgcagtat caaggtaaca acgaaggagc cagtaatggt caggaaggca
     5101 ccaataacga acgcgatgtt cgtcatgaaa acggtgacgg ctggggtctt tccacaacat
     5161 atgatttagg catgggcttt agcgctggtg cggcatacac ctcttctgac cgcaccaatg
     5221 accaggttaa ccatactgcg gcgggtggtg ataaagcaga cgcgtggact gctgggctaa
     5281 aatacgatgc taacaatatt tacctagcaa ccatgtattc agaaacgcgt aatatgaccc
     5341 cgtttggcga cagcgattat gcagttgcga acaaaaccca gaactttgaa gttactagta
     5401 ccagtttgat ttttgtctgc gtccggcagt ctctttcctg atgtctaaag gccgtgacct
     5461 tcacgctgct ggtggtgcag acaacccggc aggtgttgat gataaagatc tggttaaata
     5521 cgccgatgtt ggcgcgactt actatttcaa taaaaacatg tccacctatg ttgactataa
     5581 aatcaacctg ttggatgaag atgacagttt ctacgctgcc aacggcatct ctaccgatga
     5641 tattgtcgct ttaggtctgg tttatcagtt ctaaatcctc ctgcccgctg ttatggcggg
     5701 ctttttctgc ttattcttcc tcctttgatc taaattaaaa atgtgaactc cgtcattaca
     5761 caaaaagtgt catctggcgt tacactttat gcggatacta aaacaggagg ttttatgaac
     5821 agaacgattc ttgtccctat cgatatttca gattcagaat taactcaacg cgtgattagc
     5881 cacgttgagg cagaggcaaa gattgatgat gcagaggttc atttcctgac ggtaatacct
     5941 tcactgccct actatgcctc tctgggttta gcgtattccg cagaattacc ggcaatggat
     6001 gacctgaaag cggaagccaa atcgcaactg gaagagatca ttaaaaaatt taaactgcca
     6061 accgacagag tgcatgtcca tgttgaggaa ggctcgccca aagaccgcat tctggaattg
     6121 gcgaagaaga tccccgctca tatgatcatc attgcttccc atcgaccgga tatcaccact
     6181 tatctgctcg gttccaacgc cgcagctgta gtgcgtcacg cagagtgctc cgtgctggtt
     6241 gtgcgctgac actaacgccc gcacattgct gcgggctttt tgattcattt cgcaaatgtg
     6301 ctgacatttt cccctctaat ccgtaccata cacgccacag tttttatatc agatttctta
     6361 tgctgagcgt tccggcatga cgcatactct tctgatgcca tataacgaat tgagtcgctt
     6421 ttaaatgtcg caaaatcaag aaattagtaa gaaagaacaa tacaacctga acaaattaca
     6481 aaaacgtctg cgtcgtaacg tgggcgaagc cattgctgac ttcaatatga ttgaagaagg
     6541 cgatcgcatc atggtttgcc tctccggggg taaagacagc tataccatgc tggagattct
     6601 gcgcaatttg cagcaaagcg cgccaatcaa tttttcgctg gtggctgtta acctcgatca
     6661 aaagcaaccg ggcttcccgg aacacgttct gcccgagtat cttgaaaagc tgggcgttga
     6721 gtacaagatt gttgaagaga acacttacgg tatcgtgaaa gagaagattc cagagggcaa
     6781 aaccacttgc tcactgtgtt ctcggctacg tcgcggtatc ctttatcgca ccgcaacgga
     6841 actggggacg acgaagatcg cgttgggtca ccatcgtgac gatatcctgc aaacgttgtt
     6901 cttaaatatg ttctacggcg gcaagatgaa aggtatgcct ccgaaactga tgagcgatga
     6961 tggcaaacat atcgttattc gtccactggc ctactgccgc gagaaagata ttcagcgatt
     7021 tgccgatgca aaagcgttcc cgattattcc gtgcaacctg tgcggttcac agcctaacct
     7081 gcaacgtcag gtgattgctg acatgttgcg tgactgggat aaacgttatc cagggcgtat
     7141 cgagacgatg ttcagcgcga tgcagaatgt ggtgccgtcg catctgtgcg ataccaacct
     7201 gttcgatttc aaaggcatta cccacggttc tgaagtggtt aacgggggtg atctggcgtt
     7261 tgatcgcgaa gagatcccac tacaaccggc gggctggcag ccagaagaag atgaaaatca
     7321 gttggatgag ttacggctga atgtggttga agtgaaataa ccaggatagc gcccgatgcg
     7381 caagcgtatc gggctactct tatggaggcc ggataagacg cggccagcgt cgcatctggc
     7441 aatcccgaat aagatgttta ctcttgcacc cggcaattca acatttcatt attttaataa
     7501 ccgcacccgg cacgtttttc ctttaatctt cccgccctgt aactgtttcc atgctttatg
     7561 agcaacagcc tgacggaccg cgacatagac atgcgccgga tgcacggcga ttttgccaat
     7621 atctgcgcca tcaagcccga tatctcctgt cagtgcacct aatacatcac ccgggcgcat
     7681 tttggctttt ttcccgccat cgatacacaa cgttgccatt tctgcttcca gcgtcacaat
     7741 ggaactatta gctggcggcg tttgccagtt aagttttatc tgcaacatgt cagaaatgat
     7801 attggcccgc tgtgcttctt ccggagcaca gaaactgatc gccagaccgc tatttcctgc
     7861 acgagctgta cgaccgatgc gatgtacatg aacttcaggg tcccacgcca gctcaaagtt
     7921 caccaccagc tcaagcgatt taatatccag accacgcgca gcaacatcag tcgcaaccag
     7981 tacacgggcg ctaccgttag caaaacgtac cagggtctga tcgcgttgct ccagatcgcc
     8041 gtgtaacgac aatgcacttt gctctacttc attcagcgcg tcgcagacag cctggcaatc
     8101 ttttttggta ttgcaaaaca ccacacaaga ggatggctga tgcaagctta ataaccgttg
     8161 caacagagga attttgcctt tactggatgt ctcataaaat tgttgttcaa tgggtggcaa
     8221 agcatctgtt gagtcaattt caatcgccaa aggatcgcgt tgcactcgtc cgctgattgc
     8281 ggcgatggct tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg caggcgcaaa
     8341 acggatgaca tcatcaatgg catcgctaaa tcccatatcc agcatgcggt cggcctcatc
     8401 catcaccaaa gtattcagcg catccagtga taccgtgcct ttttgcaagt gatccagcaa
     8461 acgacccggc gttgccacga taatatgcgg cgcatgttgc agcgaatcac gctgcatacc
     8521 gaacggttga ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac gcgccagccg
     8581 acgcaattca cctgccacct gatccgccag ttcacgcgta ggacacagca ctaaagcctg
     8641 ggtttgaaat agcgacgcat caatttgctg taacaagccg aggccaaaag ccgccgtttt
     8701 gccgctgccg gttttcgcct gcacgcgaac atcttttccg gcaaggatcg ccggaagcgc
     8761 ggcggcctgc accggcgtca tggttaaata acccaactca ttaaggttcg tgagttgggc
     8821 gggaggcaaa acattcaggg tagaaaaagc ggtcacaatc tattctcgtg gtcatcgacg
     8881 caaagttagc aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa tttaatcggt
     8941 tcttcatcgg gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc gggcactgga
     9001 acaggatcgc caggaatcgg ttcagggaca ggaatttgca aataaataag tgtcgtcata
     9061 tttccctccg gtcattgggt ggactcttaa agggtagacg ctgataaata acaggcaaaa
     9121 aaaagccgac tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa
     9181 aaaggaagta agacaatatg gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa
     9241 gggagatatt gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt
     9301 tttgtataca gacagccgga aaaattgctt tttttacaac catttactac ggtgcaacca
     9361 taaagcaaca ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg
     9421 ccacccgccg ccagggatcc caccaaggtt gacgccaaat aacccggtca gaaaggtact
     9481 gggtaaaaag accattgcca tcaacgacat tgtataggta cgacgagcta aattttcctg
     9541 catcacctga gcgatttcat ccgccatcac gccagtccgt gctatacagg cgtcgatttc
     9601 gtcaaggccg cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct
     9661 catccacggc aaacgttcac tggcaagacg agcataaaca tcacgttgcg gtgccatata
     9721 gcgacgcatc acaattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg
     9781 ctgatcaagg agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc
     9841 actggaatga tcggtcaacg catcgcacac atccaccagc catcccccgc aatcggtcgg
     9901 acccgtgccc tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg
     9961 ggtcgaaaca attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg
    10021 ttcatcggtg ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac cgaaacggct
    10081 gactcggggc cgtgtgctct cgcccgccag cgcatcacgt acgttattgg gaagcagcgg
    10141 tgttgtcgcc agccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg
    10201 atgcgcttca tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag
    10261 catccaggca aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccactgt
    10321 gcctccatca tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct
    10381 catcacccca ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca
    10441 aaatgctttt ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat
    10501 tatctgatca tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata
    10561 acgggatctc aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct
    10621 ggattgtttc gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga
    10681 tgcaagacct cacgtttagt cagctacgaa ccggacccta ttccgtctcc tcacaaaaag
    10741 aactgccgaa atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg
    10801 ttcagcgtaa agaagaggaa acagcattaa gctgtcggct tgttttgcga gagctgacag
    10861 aaacagaacc ggtgattatt ttcgaaggta tcgaagcgcc ggcaacgctg ggtttaaaag
    10921 ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga
    10981 ctaactctgc accaatgttg ttgattgacc cgtcacgaga tggacaaatc gtcgatgcta
    11041 acctcgccgc gctcaatttc tatggttata accatgaaac gatgtgccag aaacatacct
    11101 aggaaataaa tatgcttggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac
    11161 ccggcggtca taaccctttg aattttgttc ataaactggc ggatggatct actcgtcatg
    11221 tgcagaccta tgccggaccg attgaaattt atggcgacaa gctcatgtta tgtattgtgc
    11281 atgatattac tgagcaaaaa cggctggagg agcagctgga acatgctgct caccatgacg
    11341 cgatgaccgg attactgaat cggcgacagt tttatcacat tacggaacca ggccaaatgc
    11401 agcatctcgc catcgctcag gattacagct tgttgctcat cgacaccgat cgttttaaac
    11461 acattaacga tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgcc
    11521 ccctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg
    11581 tcttattgct accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgcg
    11641 taagcgtggc aaaagtgagt atttcgggct taccacgctt taccgtcagc attggtgtgg
    11701 cgcatcacga aggaaatgaa agcatcgatg aactgtttaa acgcgttgat gatgctttgt
    11761 atcgggcgaa aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc
    11821 gagatcaacg actgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt
    11881 tacgtagtgc gacgtaacac gccccgctgc cgccatgatg cggtagcgcg gtgcaatatg
    11941 cctgaacatc atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat
    12001 gtgatttatc ttcccgccct ttaccatgaa taatcagcac attacgcaaa ccatccgcca
    12061 gggcttgttg aatgaaactg aacaccattt tgcggcactc ttccaccggc tggcgcaaaa
    12121 gattcaggct cgcctgttgc ggatatttac cactgcgcag cttatccagc accccatgct
    12181 acaacccttc ctgccgaaac tccagcggct gacttagcgg gatgatgtcg agaaatccgg
    12241 tagtgaggaa attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg
    12301 ttggatgcca gtggacatct gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa
    12361 gaaacagcga tttgtcgtca aggttcatgt aacctcctat gcaccttcac acgtcgtgat
    12421 tcagacgggt taattgttca tacttactgc atcgttccct tcacagattg aaacaatact
    12481 ctacttcata cctgtgagta tctgcgcagg cggggcattt tatactagcc tgttagcagt
    12541 atcgaacagt ttagcccgag tttaattcag cacaggctcc gatcaggatg atttattcat
    12601 agcaggaacc aacaaaaatg cgataaataa cagaccactg attagcagaa aggtaccgaa
    12661 aatgccatag tttgcgcctc cctcagcatc aattgactca ataatttggt tgaagccagt
    12721 atttccgatg tctggtcact tctactgcga ttaaaaaata aagcacttct ggaaatgaag
    12781 tcattcaccg tatggggaac agaaaccatt ttttcaccca ttctcgaaaa atgaaaatta
    12841 gttcgattat tgagaataga gccacacagg gcaataccaa tcgcgccccc aagattacgt
    12901 gttaaattaa atacaccaga ggctaacttc agccgttctt ttggaattcc ccccagcgtt
    12961 aatgttacga tgggggccat cgcaaactgc tgcgaaatcc ctctaattgc ctgtggaaac
    13021 agtaattcct gccatcccca ttcgtgggtt ataggggtaa aaagatacat tgaaaacaca
    13081 aatccgccca gccccgccat caacagccat cgcagattta tttttttact taaccagaag
    13141 taaaagggaa cagaaaataa ctggaaaata cctgttgtgc aaacggcaaa acctatctct
    13201 tcggcattta atccgcgaat ctgccccaaa aacacaggta ttaaatatac agtagagaat
    13261 atcccaaccc ctccagaaaa agaaaaaaaa catcccagag tgaaattttt atccttaaaa
    13321 gcatgaagat ccattatcgg attggatatt gtaagtgtac gcgcagcaaa taaaataaaa
    13381 ctcaccaatg ccagaactga tgtcagcaaa atagtattat catccagcca tccccagcgc
    13441 gctccttctt caagggtata ttccagacat cccagagtca tggccaacag gataatactc
    13501 ggataatcag caactttcag taaagagaga tcaggtttat caaaattaac cagaaaagga
    13561 atactcaaaa ctaaatatat gccaggtaat atattgatat aaaatagcca tcgccagtcg
    13621 aggttatcag tgagccagcc ccccagagtt ggccctagcg tcggtgacag tgaggccagc
    13681 gcactgacta ccgcagcagc caaccccaac tctttaccct ggtaataaat aaacgccatc
    13741 gtgaaaacta aagggatcat ggatgcgcca gcagctccct gtaaagcacg aaacaaaatc
    13801 atgatctgaa tattccatgc aagtccacac gcgattccac acgcgatact cattaaagta
    13861 aatattcccg cggatagagt aaaaagccaa cgagtggaaa atacccgtga cagccaccca
    13921 gagagcggaa taacaataat ctcagcaatt aaatagctgg tctgaagcca ggccatttcg
    13981 tctttacctg cggacagacc accacctatt tcatttaggg atgacgatac aatttgaata
    14041 tcaagctgag caataaataa tcccatacac atactggcga aagcaatatg tttacgcata
    14101 gacatcatct ctcatcaaca ctgacggtga cggacagtcc cgggcgaaga agttgcttta
    14161 attcctttgc gtcgtcaaat gcaatccgta ccgggacacg ctgaacaatt ttggtgaaat
    14221 tgccagtggc attttctatc ggaatcaagc tgaaactggc tcctgtcgct ggggaaaggc
    14281 tttcaatgtg accgtgaaac acttcccctt tgagaatatc agcacggatc tcagccttca
    14341 ttcctggctt cattcccgct atttggtttt ctttataatt tgcatcaacc cacaactcac
    14401 tgactggtac tagtgaaact aacgatgtgc cgccttccac ccaactgcct gt
//
LOCUS       DAJTUI010000092        14379 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTUI010000092 DAJTUI010000000
VERSION     DAJTUI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.092"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..133)
                     /locus_tag="OO464_003131"
     CDS             complement(<1..133)
                     /locus_tag="OO464_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6228184.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
     gene            316..459
                     /locus_tag="OO464_003132"
     CDS             316..459
                     /locus_tag="OO464_003132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228185.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(678..2216)
                     /gene="aldB"
                     /locus_tag="OO464_003133"
     CDS             complement(678..2216)
                     /gene="aldB"
                     /locus_tag="OO464_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR6228186.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2381..3532)
                     /gene="yiaY"
                     /locus_tag="OO464_003134"
     CDS             complement(2381..3532)
                     /gene="yiaY"
                     /locus_tag="OO464_003134"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR6228187.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3722..5566)
                     /gene="selB"
                     /locus_tag="OO464_003135"
     CDS             complement(3722..5566)
                     /gene="selB"
                     /locus_tag="OO464_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6228188.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5563..6954)
                     /gene="selA"
                     /locus_tag="OO464_003136"
     CDS             complement(5563..6954)
                     /gene="selA"
                     /locus_tag="OO464_003136"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6228189.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(7052..7660)
                     /locus_tag="OO464_003137"
     CDS             complement(7052..7660)
                     /locus_tag="OO464_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6228190.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7731..8816)
                     /locus_tag="OO464_003138"
     CDS             complement(7731..8816)
                     /locus_tag="OO464_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6228191.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8819..9181)
                     /locus_tag="OO464_003139"
     CDS             complement(8819..9181)
                     /locus_tag="OO464_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6228192.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9718..11631
                     /gene="mtlA"
                     /locus_tag="OO464_003140"
     CDS             9718..11631
                     /gene="mtlA"
                     /locus_tag="OO464_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6228193.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11726..12874
                     /gene="mtlD"
                     /locus_tag="OO464_003141"
     CDS             11726..12874
                     /gene="mtlD"
                     /locus_tag="OO464_003141"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6228194.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12874..13461
                     /gene="mtlR"
                     /locus_tag="OO464_003142"
     CDS             12874..13461
                     /gene="mtlR"
                     /locus_tag="OO464_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6228195.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13473..13682)
                     /locus_tag="OO464_003143"
     CDS             complement(13473..13682)
                     /locus_tag="OO464_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228196.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13966..14328
                     /locus_tag="OO464_003144"
     CDS             13966..14328
                     /locus_tag="OO464_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR6228197.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         3517 a         3827 c         3615 g         3420 t
ORIGIN      
        1 gaatggcgtc ggcatgagga tgattacgtt ttttagcaat caggtaggga atatcatgta
       61 aaataataat cccatagaat atcaccagaa atacaaaaat aagcactccc agtgcaaaat
      121 agtccaggaa catatttccc tcaaagaata taaaaaagaa caattaacgc atattatgaa
      181 atgccatgat gcagtgcaat ttccttattt atataacgac atattgtttt ttatatactt
      241 tctgaaatgt gctgtctggt ttttcaacag gacgaggttt atcatattga aatagtggaa
      301 ctattggcca aactaatgaa taactccagt aaaacatcca cagtacagat taagcgtatt
      361 aaaccttcaa ttatctaccg tttattgctg attggcctcg gatcaccaat ggtgatttac
      421 ggcctggttc gcccgctcac catcgaaacg cgagattaaa ctcctgacaa aacacgcccc
      481 agaaacgcac ttctgttggc gtagtctggg ttattgcgca cgtaggttta aaacgtaaat
      541 tgtaacaacg tgcgctttgt ttatgccgga tgcggcgtgt acgccttatc cggcctacgg
      601 tcaggttccc gtaggcatga taagacgcgt aagcgtcgca tcaggcaatg aatacccaat
      661 gcgaccagct tcttatatca gaacagcccc aacggtttat ccgagtagct caccagcagg
      721 cacttggttt gctggtaatg ctccagcatc atcttgtggg tttcgcgacc gatacctgat
      781 tgtttgtagc caccaaacgc agcatgtgcc gggtaagcgt gataacagtt ggtccacacg
      841 cgcccagcct gtatgccgcg ccccatctta taggccagat taccgttgcg gctccagacg
      901 cccgcgccca ggccatattg cgtatcgttc gccagctcca gcgcttcttc catcgttttg
      961 aaggtggtca ccgccagcac cgggccaaaa atctcctcct ggaacacccg catattgttc
     1021 tgaccaaaca gaatcgtcgg ttcgaggtag tagccgtctt tcagttcacc ttccagcagc
     1081 ttgcgccgcc cgcctgtgag cacgtcagcg ccctcttttt taccgatatc aatgtagttg
     1141 aggatggttt ccagttgccc gtgagaaacc tgcgcgccca tttgcgtcac gctgtcgagc
     1201 gggttaccgc tgcgaatgct ttcgacacgg cggatggcgc gttccataaa gcgttcgtag
     1261 attgactcct gcactaaggc acgactcgga caggtgcaaa cttcgccctg gttaaaggca
     1321 aacagtgcaa agccttccag cgctttatcg aaaaaggcat cttcttcatc catcacatca
     1381 gcaaagaaga tatttggcga cttaccgccc aactccagcg tcaccggaat aatgttttgc
     1441 gttgcgtatt gcataatttg ttggcccact tccgttgagc cggtaaacgc cactttggcg
     1501 atgcgtttcg aggtcgccag atattcgcca atttccccac ctgcgccatt gaccacgttc
     1561 accacgcccg gcggcagtaa atcaccgaca atttccatta gcagcagtac agaaagcggg
     1621 gtaagacgtg cgggtttcag caccacacag ttgcccgccg ccagcgcggg agccattttc
     1681 cagctcgcca tcagcagcgg gaagttccac gggataatct gccccaccac gcctaacggt
     1741 tcatggaaat gataggccac ggtttcgcta tcaacttcac tgatcccacc ttcctgcgcc
     1801 cgaatacacg aggcgaaata gcggaaatgg tcaatcgcca gcggtacatc cgcagcactg
     1861 gtttcgcgaa tgggtttgcc gttatcccag gtttcagctg tcgctaacag ctcgaggttt
     1921 tgttccattc gatcggcaat cttaaacaga atcgccgcgc gatcctgcac cgaggtgtgc
     1981 gcccatttat ctttcacttt gtgcgcagca tccagcgcca gatcgatgtc tcgtttgccc
     2041 gaagacgcca cttcgcacag cagctgcccg gtcaccggcg tcagattctg gtaatactca
     2101 ccatcggcag gggctaccca ttcaccgcca ataaagttgt cgtagcgggc ttttaacttg
     2161 atggggaaac catactcgcc gggcttaatc tgtgctgaag ggggattatt ggtcatgatc
     2221 gtctccttgc ggtgtgaggt ataacaaggg tagacgtgac tggcgaaatc ttcgccagtc
     2281 ggtaacagct ttacgacagc tatcacgaat ttacgggcaa ggattacaga gatagttgaa
     2341 aaagcggctg acaatttgcc agccgttgtt ggaatgatga ttacattgcg gcgcgataaa
     2401 tcaccacaat ttcttcgtga gttgcctgga tcgggttagt aaagccacag gcatctttca
     2461 gggcattagt cgccagaacc gcgaaatctt cttctttcac gttcaggtcg cgtaggcctg
     2521 ccgggatatc cactttcttc gccagttcac ggatggcgtt aatgcaggct tcagcacctt
     2581 ccgcgtcgtt tttacctgtc acgttcacgc ccattgcagc ggcacagtca cgcagacgtg
     2641 cagcggcgac tttgctgttg aatacctgaa catgcggcag cagaacggcg ttacatacac
     2701 cgtgtggcag gttgtagaaa ccgcccagct ggtgcgccat cgcatgaaca taacccagag
     2761 aagcattatt gaacgccata ccggcaagga actgggcata agccattgct tcacgcgctt
     2821 tcgcattact gccatcttca acggctaacg gcaggttttc ggcaatcatg gtcacggctt
     2881 tcagtgcaca agcgtcagtg atcggcgtgg cggcaataga aacatacgct tcgatagcgt
     2941 gcgttaaggc atccataccc gttgcggcgg tcagtgactt cggcatacca atcatcagag
     3001 aggagtcatt gacagaaagc agcggagtca cgtgcttatc aacaatcgcc attttgttat
     3061 gacgcgcttc gtcagtgatg atgcagaaac gggtcatttc agaggccgta cccgccgtgg
     3121 tattgatggc gatcatcggc agctgcggtt ttgcagagcg gtcaacgcct tcgtaatcgc
     3181 gaatatcgcc gccattggct gccaccagcg caataccttt tgcgcagtcg tgtggagaac
     3241 caccgcctaa ggagatcacg ctatcgcaat tattttcttt aagtaatttc aaacctgcgg
     3301 cgacgttttc cgtggtgggg ttaggttggg tgccatcata aataacgcta aaaatattgc
     3361 gttcttccag tgctttttgc acatcacccg ccatacctaa tttcgttaac atattgtcag
     3421 tgacaattaa ggtacgggta aatccataat ctgccatcat attcattgca tcagtcaatg
     3481 aatcagcgcc gatgacattc acagaaggaa taaagaaagt tgaagatgcc ataacacact
     3541 cctgaaagtg taaaaaaggg tgggcagctt actgaggatt ttcattcgaa aacatgatca
     3601 ttattattaa ttatcaaaat taatctaata aaaagcacat tgtattcatt aaacaatgtg
     3661 ctttatttaa aatcataaga aatttgtatt aatttatttt aaaaattaat taatcatttc
     3721 cttatttttc cggaaataat aatgcgtcgc gcaataaatg atcatttcca cgacgacgcg
     3781 taaagccaat gcggtcaaaa tattccagaa tctgaattgc cagctttcgg cctacgccta
     3841 agcgatcgcg gaaatccgcc gcgcaggttg aaccacactc ctgatcgaga tcgcggatca
     3901 tattggcaaa ttcgacaatc cgatcgttac ggtaataacg atctttgacg atcgcggtaa
     3961 ttattccttg ctgcgccgcc tggcgtagcg tcaggcgcat tgcctgctcg tcggtacccg
     4021 tctcttttgc cagatcacgc acccaccacg gctcgtcacc aaacagcggc tctgcttttt
     4081 gccagatggc ctgctgctct tcgctgaagc ccgctttgtg atccggcaga tgcagccaac
     4141 cgtgatgact gaggatgtcg ccgctttcgc gcatcttttc aatcagcaac agtactagcg
     4201 cttcatcttc cattggcaac gccatacgtc gcagacgttc gcgcccaggc ccaggttcat
     4261 cgcgatgttg ctcatgataa gtcgctaatg tgtcgagaat tttccgttgc cagcgagcgg
     4321 caaccggcgc attcaacaag ctataaccag cctgaatata gccaggctgt tgcagcagtt
     4381 cgcgcatccc ttcgccgttg agctggcgcg cccaagcgaa atcaggaaga ttaaccgcgc
     4441 cgcgttgcag gtgaacggct aacgcttcgc tatcattctg tgccctggcg agggaagcca
     4501 gccattgcag atactctggt ttacgtttac cgcgacgcgg cgggttaagc atcacgacgc
     4561 gcgctccggc cagcgtgttg cgggcagaga tatcgcgcaa taccaggcgg tcgttatctg
     4621 ccagccataa cggggtgtcg aagaccagtt cagcaaggtt atcttccagc agtgaaacgc
     4681 gtcccgtgac gtggctggcg gcgtggtgaa tatgcagcgg ctgccactgg gtcagcggtg
     4741 catgggtttg aagctcgaca atcacccgtg tgaacggctc tggcggcaca tcggcaagca
     4801 gccagtcgcc acggttaatc tgctcttttt ccgcatcacc cgcgatgtca agcgcgatac
     4861 gctgcccggc attggcggtt tctgttggct ggttttgcgc atgcagcgca cgtacacgca
     4921 tcggtttatt tacaccagtc agccagagtg aatcgcctac cttcacttcc ccgcttaacg
     4981 ccgtaccggt gacgaccagc ccggcacctt ttacggtaaa tgcgcggtca atcgcgaggc
     5041 ggaaactatg ttggctggcg tgctcgcgtt ccggcaactg aagcagatgc tcgcgcaggg
     5101 catcaattcc ccgaccttcg gttgctgcgg tgataaacag ttttgcctca gcaaaaccgt
     5161 attcccgcag aacctccttt acctggcgtt caacctcatc aacacgcgct tcgtccacgc
     5221 gatcggcttt ggtcagcgcc actgtcagca tcgggttacc ggtcagctgc aaaatcgcca
     5281 gatgctcacg ggtctgtgcc atcacgccgt catcgcacgc caccaccaac agcgcgtgat
     5341 cgataccacc aacgcccgcc agcatgttgg aaagaaactt ttcatgaccg ggaacgtcga
     5401 taaaaccagg cacgcgacca tccggctgcg gccagtaggc atagccgaga tcgatggtca
     5461 tgccgcgctt tttttcttcc ggcagacggt cagcatttac gccagtaatc gcctgcaata
     5521 aggttgtttt gccgtggtca acgtgtccgg cagtcgcaat aatcatttca acaacatctc
     5581 caaaaaccgt tgctcatctt caaggcagcg taaatccagc cacaatcgtc cgtcataaat
     5641 acgaccaatc accggcactg gcaattcacg ccagcgggcg gctaatgact caaggtggct
     5701 accgcgtcca tcatggggtg taaacgttaa tgccgcgctc ggcaggcgat caaccggcag
     5761 cgaaccactg ccaatctgcg aaagacatgg cataacctgt accgcaaact ccgcgccgta
     5821 atgtgcgaca aggggagcct gtaaacgttg tgcctggatt tgaatgacct ctgcgctgcg
     5881 ggtaagcagg cgcagggtcg gtaatttttc actcagagct tcagggtgta aataaagacg
     5941 caacgtggct tccagcgccg cgagggtcat tttatccgcg cgtaatgcac gcttcagcgg
     6001 gtggctttgc aggcgggcga tcatctcttt tttaccaacg ataatcccgg cctgcggccc
     6061 gcctaacaac ttgtcgccgg agaagctcac cagactgacg cccgccgcaa tcaactcctg
     6121 cggcattggc tctttcggca aaccgtactg gctaagatcg accagcgagc cactgcctaa
     6181 atcagtcacc actggaacat ccagctcttt gccgagcgcc accagttcag cttcgtctat
     6241 cgctttggtg aacccctgaa tgctgtagtt actggtatgt actttcatca acagtgcggt
     6301 attttcattc accgcctgac gataatcatt cgcgtgcgtg cggttggtgg tccctacttc
     6361 gtgtagggtg cagcctgcct gacgcataac atcgggaata cgaaacgcgc cgccaatctc
     6421 caccagttcg ccgcgagata ccaccacctc tttcccgctg gcagtggccg ccaacatcaa
     6481 taacaccgcc gccgcattgt tattgacgat acaggcatct tccgcccccg taatacggca
     6541 cagcagctgc gccagcgccc gatcgcgatg tccgcgtccg gcgtcgtcca gatcgtactc
     6601 gagggtcact ggcgaacgca tagcctgcgc aacggcttcc accgcggctt ccgcctgtaa
     6661 agctcgccca aggttggtat gcagcacggt tcccgtcagg ttgatcaccg gacgcagcgc
     6721 gctctgcgcc tctttcgtca accgggcatc gacttcttgc gcccagtttt cacaccacgc
     6781 aggcagcgtc tggctgccac gaatcacttc tcgcgcttcg tcgagcatct gacgcaacaa
     6841 ttccaccacg cgggtgtgac cataagtatc acgcaaagaa aggaaggagc tatcgcgcaa
     6901 taagcgatca atagccggaa gttgactata gagggaacgc gtttcggttg tcataggaaa
     6961 cctggctgat caaggccctc tcacacggag aagggcgttt aacataacca cggattgtaa
     7021 cgtgagatgg gtcaggagga catatcgcgc atcaagcctt tggcggttcg gtgcgagcaa
     7081 aactttcgcg gctaaacagg ttttcgacca gtttgactaa gtgtgggcgg tcgacacacc
     7141 aacctggcgc aacgcggcgg aaattgagat atccgacagc acaggcaatg gcgatagtcg
     7201 ccagattgac cgtatcggtt ttgagtgtgc catcgaccag atatccttcc agcacatcca
     7261 gactgcggtt gattttctcc cgctggcgta acaattcatc ttcagactgc tgcgccgccg
     7321 gacgcgcctg ttcacgcacc gataccagcc cggcatccat aatgccatcc gccagcgcct
     7381 caattttgcg cacccgcaac gactccagcg gatcgcgcgg caacatcgcc ggagcgacat
     7441 tcattaactc aatatattca gcgatgatcg gcgaatcaaa ccagcattcg ccctcttcgg
     7501 tcaccagcac cggcactttc cctaacgggt taaattgcgc cacgccgtta tccgcgttat
     7561 agggcagttc attaataaat tcgaaagtta tgcccttttc taacaacaga ataaaaagtt
     7621 tgcgtacaaa cgggctggtg tagctaccga cgagtttcat gccgagtcct ttgtgcgagg
     7681 aaaaatatca gtatggctca ggctcacgca aaacacaggt gatataagtc tcaatgatcc
     7741 aaataaagat aatgcatcca actggtcatt cttagcagca ctttccgcat caccgaaaca
     7801 tggctgaaat ggtcggagta aaccgccacc tgggcgtaga tgccgtcggg taaggcatcg
     7861 atatcatcgt tagggtccag ttcaatggtt cccagcacac cgtccgtgcc gggaacgacc
     7921 gttaatgatt gcaatacccc ctgcgcctga taagaaccgc ctggcacgac aggtaaaata
     7981 ctagtcaatt tgccgtggaa cacctgccca ggtagcgcgt taaacaccac ttccgcatca
     8041 tcgccaggtt tcagacgtaa cagcgagttt tgccgaaatt gggcgacaat ttgccgtttt
     8101 tgctcgggga tgaagaccat caccggacgc agcggcaagg cagctgcgta tgtacctggg
     8161 cggatcagta cctgagtgac gtagccattg cttggtgcgc gaatgacagt ctgctcaaga
     8221 ttatattttg cttcagtaag ttgcgctctt aagctcacaa tctgcgattg ctcgccgttg
     8281 accatactgt cgagctgact ctggatctgc gcctgctccg ccaccgagcc tttcactaac
     8341 gcatcctgcg cgaggaaatt ttgccgcgca tcgtcgatgt cacgttccga gaacggattc
     8401 accgccgcct ggctgccttt caggtaacgt tgataatttt taaacagacg gtcgcgctcc
     8461 gctgaaacct gggtggtgtt ggcctgcgct tcagtgagct gcgcacgcag cgtctttata
     8521 ttatgcgtcg ccgtcatcag gtcagcctga agtctgtcaa ctcgcgcctg gtaacgaaca
     8581 gggtcgagct taaaaagcac ctcgcccttt tgaataagct gattattctt gtcagtgact
     8641 tcagtaacaa ttcccgtcac ctgtggcgtg atagggatcg ctatcactgc cttttgcgcg
     8701 gtaaaagtgt aagggtggtt gtagttcatc aacaaaatca aaccactcac cagaaacacg
     8761 cctcccagcg ccgccgtcgc cagcgccacg taagttaaaa caatcaatag atccataatt
     8821 agatctccgg gaacgtggaa tagtcagtct ttttctcggc ggcgagttga tgctccagcc
     8881 gggaaattcg gtcagaaagt gcggcgattt ccggttcagt cgctttctcc tgcgacgcaa
     8941 catgtgactg cataccccag ccacgctccg gttgatagag cgtcgcccag atccacagaa
     9001 acggccagat aacatgcaga gtaaacaggc tcacccagcc cgccgtatga atagcgtcgg
     9061 catgggggtg attgcgcttt ttggcaatca gataagggat gtcatgtatc gcgatgattc
     9121 cataaaaaat caccagaaag acgaagatca gcactcccaa cgcgaaatag tttagaaaca
     9181 tatctgcctc ggattcacgt ttatcagtgt tgttttttgg ctggcagcca gaagggagtc
     9241 aggctgatat tttgacaata atccgggttc gcgattctcg ccataacacc aaagaataat
     9301 ttttagaggt gatgagttgc ttagttacat aacgattgta tgacgaaggc ataacatgct
     9361 gtagatcaca tcaggtgaac gccgtaagaa aatatcttgt gattcagatc acaaagattc
     9421 aacaaaccat caaaacaaaa atgtgacatt actcacattt aaatgccatt tttagcgaaa
     9481 atcaccgcct tgtttctttt ttatacaggc gttttgtgat gagcgtcacg tcaattacct
     9541 ctctaccccc tatatttatg tgactgatat cacacaaaag cccgtcgact ggacagttaa
     9601 ccgattcagt gccagatttc gcagtatcta caaggtccgg ctacctctgc cgccacatta
     9661 acaaaaaacc tcgggcttcc agcctgcgcg acagcaaaca taagaagggg tgtttttatg
     9721 tcatccgata ttaagatcaa agtgcaaagc tttggtcgtt tcctcagcaa catggtgatg
     9781 ccaaatatcg gcgcgtttat cgcgtggggt atcatcaccg cgttatttat tccaacaggg
     9841 tggttaccga acgagacgct ggcgaagctg gtcgggccga tgatcactta tctcctaccg
     9901 ctgctgatcg gttataccgg tggtaagctg gtaggcggca aacgtggtgg cgtagtcggt
     9961 gccattacca ccatgggcgt tatcgtcggc gcagacatgc cgatgttcct cggttctatg
    10021 attgcaggtc cgctgggcgg ctggtgcatt aagcacttcg accgctgggt agacggtaag
    10081 atcaaatccg gttttgagat gctggtgaat aacttctccg caggcatcat cgggatgatc
    10141 ctcgctattc tggcattcct cggcattggc ccgattgttg aagccctgtc caaaatgctg
    10201 gctgcgggcg ttaacttcat ggttgtccat gacatgctgc cgctggcgtc tatctttgtt
    10261 gaaccggcga aaatcctgtt cctcaacaac gccattaacc acggtatctt ctcgccgctg
    10321 ggtattcagc agtcccatga actgggtaaa tcaatcttct tcctgattga agctaaccca
    10381 ggtccaggta tgggcgtgct gctggcgtac atgttctttg gtcgtggtag cgctaaacag
    10441 tctgcgggcg gtgcggcaat catccacttc ctgggtggta tccacgaaat ctacttcccg
    10501 tatgtgctga tgaatccgcg tctgatcctc gcagtcatcc tcggcggtat gactggcgtg
    10561 ttcacgctga ctatcctggg cggtggtctg gtttctccgg catctccggg ttctatcctt
    10621 gctgtactgg cgatgacacc aaaaggtgct tatttcgcta acatcgcggg tgtgtgtgcg
    10681 gcgatggctg tctccttcgt tgtctctgct attttgctga aaaccagcaa agtgaaagaa
    10741 gaagatgata ttgaagcagc aactcgtcgt atgcaggaca tgaaagctga gtctaaaggc
    10801 acatctccgc tatctgcggg cgatgtgact aacgacctga gccacgtacg taaaatcatc
    10861 gttgcctgtg acgccggtat gggttcaagt gcgatgggcg caggcgtgct gcgtaagaaa
    10921 attcaggatg caggtctgtc gcagatttcg gtcactaaca gcgcgatcaa caacctgccg
    10981 ccagatgtgg acctcgtcat cactcaccgt gacctgaccg aacgcgctat gcgccaggtt
    11041 ccgcaggcac agcatatttc gctgaccaac ttcctcgaca gcggcctgta caccagccta
    11101 accgaacgtc tcgttgctgc ccagcgccat actgaaaacg aagtgaaagt gaaagacagt
    11161 ctgaaagaca gctttgacga ttccagtgct aacctgttca aactgggcgc ggagaacatc
    11221 ttcctcggtc gcaaagcagc aaccaaagaa gaagcgattc gttttgctgg cgagcagctg
    11281 gtgaaaggcg gttacgttga gccggaatac gttcaggcga tgctggatcg tgaaaaactg
    11341 accccgactt atctgggtga gtctatcgcg gtgccacacg gtacggttga agcgaaagat
    11401 cgcgtactga aaacgggcgt ggtgttctgc cagtacccgg aaggcgtgcg cttcggtgaa
    11461 gaagaagatg acattgcccg tctggtgatt ggtattgcag cccgtaacaa cgagcacatt
    11521 caggttatca ccagcctgac caatgcactg gatgatgagt ccgtcatcga gcgtctggca
    11581 cacaccacca gcgtggatga agtgctggaa ctgctggcag gtcgtaagta atccaatcct
    11641 accctctcct cacggagagg gtttgggtga gggaaaagcc tcaccccagc cctctcgggt
    11701 aaaaacattg atgaaggtta atactatgaa agcattacat tttggcgcag gtaatatcgg
    11761 tcgtggcttt atcggtaaac tgctggcaga cgcgggtatc caactgacgt ttgccgatgt
    11821 caatcaggtg gtacttgatg ccctgaatgc ccgtcatagc tatcaggttc atgtggtcgg
    11881 tgaaaccgag caggtagata ccgtttccgg cgtcaatgct gtcagcagca ttggtgatga
    11941 tgtcgttgat ctgattgctc aggtcgattt agtcactacc gccgttggcc cggttgtgct
    12001 ggaacgtatt gctccggcta tcgccaaagg gctggtgaaa cgcaaagaac aaggtaatga
    12061 atccccgctg aacatcatcg cctgtgaaaa catggtacgc ggcaccacgc agctgaaagg
    12121 tcatgtgatg aacgccctgc cggaagacgc caaagcgtgg gtagaagagc acgttggctt
    12181 tgtcgattcc gccgttgacc gcatcgtacc gccttcggct tcggcaacta acgatccgct
    12241 ggaagtgacg gtagaaactt tcagcgaatg gattgtcgat aaaacgcagt tcaaaggcgc
    12301 actgccgaac atcccaggca tggagttaac cgacaacctg atggcatttg tcgaacgtaa
    12361 actcttcacc ctgaacacgg gtcatgctat aaccgcgtac ctcggaaaac tggccggtca
    12421 tcagaccatt cgtgacgcta ttctcgacga gaaaatccgc gcggtggtaa aaggtgcgat
    12481 ggaagaaagt ggtgcggtac tgatcaagcg ctacggcttt gacgcagaca aacatgcggc
    12541 gtacatccag aaaatcctcg gtcgttttga gaacccgtat ctgaaagatg atgtagagcg
    12601 cgtaggccgt cagccgctgc gtaaactgag tgctggcgac cgtctgatca agccactgct
    12661 cggtacgctg gaatacagcc ttccgcacaa gaatctgatt caggggattg ctggtgcaat
    12721 gcacttccgc agtgaagatg atccgcaggc tcaggaactg gcagcactga tcgctgacaa
    12781 aggtccgcag gcggcgctgg cacagatttc cggtcttgat gccaacagcg aggttgtatc
    12841 cgaggcggta accgcttata aagcaatgca ataatggtgg accaggcgca ggacaccctg
    12901 cgcccgaata acagattgtc agatatgcag gcaacaatgg aacaaaccca ggccgttgaa
    12961 aaccgtgtgc ttgagcgtct gaatgctggc aaaaccgtgc gaagctttct gatcaccgcc
    13021 gtcgagcttt tgaccgaggc ggtaaatctt ctggtgcttc aggtattccg caaagacgat
    13081 tacgcggtga agtatgctgt agaaccgtta ctcgacggcg acggtccgct gggcgatctt
    13141 tctgtgcgtt taaaactcat ttacgggttg ggcgtcatta accgccagga atacgaagat
    13201 gcggaactgc tgatggcatt gcgtgaagag ctaaatcacg acggcaacga gtacgccttt
    13261 accgacgacg aaatccttgg accctttggt gaactgcact gcgtggcggc gttaccaccg
    13321 ccgccacagt ttgaaccagc agactccagt ttgtatgcaa tgcaaattca gcgctatcaa
    13381 caggctgtgc gatcaacaat ggtcctttca ctgactgagc tgatttccaa aatcagctta
    13441 aaaaaagcct ttcaaaagta agcaacgtct gcttactgcc cctctacctg cttcggccga
    13501 taaagccgac gataatactc cagacgttgt agatataacg gcacgctttc atcgggtatt
    13561 ccggacggaa tcgcgttacg gggaggaagt tttttcagat actcccggaa cgcctggctt
    13621 gatgccatga aatctacggc tttatcgata agaagcggaa cgttttcacc taatttgccc
    13681 atgatgatat ctccgtatta ccccgcgccg ggaatgcgcg gccgccaatt ttagtttagg
    13741 ttcccggtaa agatgacatt aaggaaaacg tgctgaatcc tcaataatca tccgctgctg
    13801 tttattatct ttttctatag atttcttaat taatcaacga attgcgtagt ttttgagcat
    13861 gttaatgatt acgttgcaga actattacaa tgtcatcagt tgtgaacaaa gcacctggtc
    13921 gcgcatacta ggggctataa atttatcttt atcagaagcc atcacatgaa agaagtcgaa
    13981 aaaaacgaaa tcaagcgtct cagcgatcgc ctggacgcca tccgccacca gcaggccgat
    14041 ctgtcgctgg ttgaagccgc agacaaatat gccgagctgg aaaaagagaa agccacgctg
    14101 gaagcagaaa ttgctcgcct gcgtgaggtt catagccaaa aactgagtaa agaagcacag
    14161 aagctgatga agatgccgtt ccagcgcgca attaccaaaa aagagcaggc tgatatgggc
    14221 aagctgaaga aaagtgttcg cggactggtg gttgtgcacc caatgaccgc gctggggcgc
    14281 gaaatgggcc tggaagagat gactggattt tctaagacta cgttttaagc ccctcagacg
    14341 actggttcga tcgtatcaac ccgcatctgg cttgcctta
//
LOCUS       DAJTUI010000093        14035 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTUI010000093 DAJTUI010000000
VERSION     DAJTUI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.093"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..2770
                     /locus_tag="OO464_003145"
                     /pseudo
     CDS             1..2770
                     /locus_tag="OO464_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            2780..6426
                     /locus_tag="OO464_003146"
                     /pseudo
     CDS             2780..6426
                     /locus_tag="OO464_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6487..6894
                     /locus_tag="OO464_003147"
     CDS             6487..6894
                     /locus_tag="OO464_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228198.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7436..8524
                     /locus_tag="OO464_003148"
     CDS             7436..8524
                     /locus_tag="OO464_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR6228199.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8535..10814
                     /locus_tag="OO464_003149"
     CDS             8535..10814
                     /locus_tag="OO464_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228200.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            10807..11943
                     /locus_tag="OO464_003150"
     CDS             10807..11943
                     /locus_tag="OO464_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6228201.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            11940..13943
                     /locus_tag="OO464_003151"
     CDS             11940..13943
                     /locus_tag="OO464_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228202.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3459 a         3692 c         3602 g         3282 t
ORIGIN      
        1 aaaatagtgg cgactgatga gcacattctt gcctcattag aaaaatatca cgagccatat
       61 gccatttttg atgattacta ttgtggtgcg atatggtcag ccaccgtatt acaggagcaa
      121 ggtgttgcag ccctgccccg atttgctccc tatgccgcaa gtgactactg cgccgatgtg
      181 ttgcgtcata tcaatcatcc gttcgcatta acactgctta tacgtgtagc cgggcatact
      241 aaacgctgtc acgatcgaat gacgaaagcc tgcgctgcgt tcccacacgc agcactggca
      301 gcactggcgg aacttctagt gcaaaaagaa gagaatagtt ggcgcattat gctaatgacg
      361 atgtttatct cgcaaccagc actggcagaa caggtcattc cctggctttc gacacccgca
      421 gttgcagtgc tgaaatcatg ccagcaccaa ctgacacagc cctcaaacca tgccagcgcc
      481 gatctactgc cagccatagt agtctcccca ccatggattt cgaaaaagaa aaaatcgccg
      541 attccggtgc tggatttagc gccattaggc atcgagccaa tctgttatct gacagaagaa
      601 atcagtaatc aacttttggc gaaatatatc tggtattcaa aacacatcac ggttagccat
      661 gaagaaagta ctaccaacct gttggcaagg atgggttttc aacgacggat cgctggtaca
      721 tatattaaag ctcccgaagc ggtagttgag gcatggctaa atgaagatta ttcaacctta
      781 ctaagtgaat ttaaggtgtt tcattcacct accgggcatt attggcagtt ggggattttg
      841 acaacattgc cgctggaggc tggagaaagc agtaaaagca tggaatgccc ttactctatc
      901 tccacatacc gataccgaat acgccatgtt acattttgga ctcaaaggtt tacctggttt
      961 agttaactca attacacgct atccacagga agccttgccc atcacgaatt acttcgcagc
     1021 gagtgagctg gctcctgccg tcgcccgtgc cttcaacaaa ctcaaaacgc tacgcgaaaa
     1081 cgcccgcacc tggctgttga aatacccgga acatgccctt accggcctgc tgcctgcggc
     1141 gctcggcaaa gccggtgaag cacaggataa cgcccgcgct gccttgcgta tgcttaccga
     1201 aaacggtcat cagccattac tgcaagaaat cgcccggcgt tataaccagt cggaagtaac
     1261 agacgcggtg aacgctctgc ttgcgctcga tcccttagat aatcacccga caaaaatccc
     1321 cactcttccg accttttatc agccatcgat ctggacgcgc ccggtattaa aagcaaacgc
     1381 ccaatcactg ccagataacg ccctcctcca cctcggcgaa atgctccgct tccctcagga
     1441 agaggctctg tatccgggat tattgcaggt gaaagacgcc tgtaccgctg actctctggc
     1501 tgaatttgcc tgggatctgt ttaccgcctg gcagaccgct ggcgcgccgt cgaaagagag
     1561 ttgggcgttc actgcgttag gcgttctcgg taacgatgac accgcccgca aactgacgcc
     1621 attaatacgc gcctggcctg gtgaatccca gcataaacgc gccaccgttg ggttggatat
     1681 tctcgccgct atcggtagtg atatcgccct gatgcagctt aacggcattg ctcagaaact
     1741 gaaattcaaa gcattacagg agcgggcaaa agaaaagatt gccgacattg ccgagagccg
     1801 cgaactcacg gtagcggagc ttgaagatcg gttagcaccg gatctcggtc tggatgataa
     1861 cggttcgttg ctgctggatt ttggcccacg ccagttcacc gtcagctttg atgaaacctt
     1921 aaaaccgttt gtgcgtgatg tttccggcag ccgcctgaaa gacctgccaa aaccaaacaa
     1981 aagcgattat gagtcacagg cgaacgatgc tgttaaccgc tacaaattgc tgaaaaaaga
     2041 tgcgcgtacc gtcgcggccc agcaggtagc aaggcttgaa tccgccatgt gcctgcgtcg
     2101 ccgctggtcg ccggaaaact tccaactctt cctggttgtg catccgctgg ttcgccactt
     2161 aacccgccgt ctgatttggg gcgtttatag cgccgacaac cagctactgg cctgtttccg
     2221 ggtagcggaa gataacagct acagcaccgc tgacgatgat cttttcaccc tgccggaagg
     2281 cgatatctct atcggcattc ctcacgttct ggaaatatca ccgacggatg ctgtcgcctt
     2341 tggtcagctt tttgccgact acgaactgct accaccgttc cgtcagcttg accgtaacag
     2401 ttacgccctg acagaagccg agcgcaacgc cagcgaactg acccgctggg caggcagaaa
     2461 atgcccgagc ggccgggtca tggggctggc aaataaaggc tggataaagg gcgaaccaca
     2521 ggatggaggc tggatgatca aacctctcgg tcgctggtcg ttaatcatgg aaatcgacga
     2581 aggttttgcg gtgggtatat caccagccga acttagcgct gagcagctct taagcaagct
     2641 gtggctatgg gaaggcaaag cggaaagcta tggctggggg agtaattcaa cacaggaagc
     2701 gcagttctcc gtactcgatg ccatcaccgc cagcgagcta attaacgata ttgaagccct
     2761 gtttgaataa ggaaagagca tggacaagga attaccgtgg ctggcggata acgcccaact
     2821 ggaactgaaa tataaaaaag gcaaaacacc actcagtcat cgcaattggc cgggcgaacc
     2881 agtgcctgtt atcactgaaa gcatcattca gacactgggt gatgaattgc tacaaaaagc
     2941 tgagaagaaa aaaaacattg tctggcgtta tgaaaatttt tcaccggagt ggcagtccgc
     3001 cattacgcag gccatcaact tgatcggcga acacaaaccc tcagtcccgg cccgcacaat
     3061 ggcggcacta gcctttatcg cgcaaaatga caaccaacag ttgctcgacg aaatcgtcca
     3121 acaagagggg ctggaatatg cgactgaggt ggtgattgca cgccagttta ttgcacggtg
     3181 ttatgagagt gatcctctgg tagtcacatt gcagtatcag aacgaggact atggctatgg
     3241 ttatcgctca gaaacctata acgaattcga tctccgactg cgtaaacacc tctctctggc
     3301 agaggaaagc tgctggcagc gttgcgccga caaactcatt gccgcactgc caggaataac
     3361 caaagttcgc cgccctttta ttgcgctgat cctccccgaa aaaccagaaa tcgccaatga
     3421 gttggtaagc cttgaatgcc cgcggactca ttttcattct aaggagtggt taaaagttgt
     3481 tgctactgac cccaaagcgg tgagaaaact cgaacgctac tggagccagg atatatttag
     3541 cgatcgagaa gccagctaca tgtcgcatga aaaccacttc ggctacgcgg cctgcgctgc
     3601 ccttttgcgc gaacaaggac tggcagtcgt tccgcgcctc gcgatatatg cccataaaga
     3661 agattgcggc agtctgctgg tacaaattaa ccatccgcaa gtcatccgca ccttgctgtt
     3721 agttgctgat aaaaacaaac ccagcctgca acgtgtggct aaatacagta aaaacttccc
     3781 ccatgcgaca ctcgccgcac tggcagaact gctggcgtta aaagaaccac cagcccgccc
     3841 aggttatcca atcatcgaag acaaaaagct gcctgcacag caaaaagctc gagatgaata
     3901 ctggcgtacg ctgttacaaa cgctgatggc atcgcagcca caactggcag cagaagtgat
     3961 gccgtggtta agtactcaag cccgggcagt gctgaatagt tatttatcgg caccgcccaa
     4021 accagttatt gatagcaccg ataacagcca aatgcctgaa atcctcgttt caccaccatg
     4081 gcgtgggaga aagaaaaaga cttccatacg tcacttttcg ctgcctgatc ttacgcttgc
     4141 accgcaaacg tacaggtcgc cgaataaaca ctggacgccg gaacagcaaa ctgcaattga
     4201 ccattttagc gccatgtctt ttgacgaacg tcttgctacg agaggagcgg atatcttctt
     4261 gcgggaatta ggttttgacg ttcatagcct ggaatttaat aagcgtcttc ttaatggaca
     4321 gttggatgcc tttgatgctc agcaactgca agaatacaca tcaataggac tattgcgtac
     4381 ttatcacgcg catcaggcca aatctaaaca gtaccgggaa gacgccacca atgcattact
     4441 ggcacaagat agcattgcgt tgatgggagg gtggcgaact tttcagcacg gattttacta
     4501 tggtaatgaa cagcgttggg ggatttggaa tctgtacctt atcgcgcaga tgccgcgcga
     4561 aatggcggta tcgtgctggc aacaaattgt cgaggctgat tttcattaca ccggggtgga
     4621 atatttgtta agtgtgctgg gtaccgatgc tttgcctgga ttaaacaccg cctttgcccg
     4681 acatccaaaa gagatatttc cgctgctgat tcatttcggc gcaacagaac tggccctgcc
     4741 cattgcccgc gtctggcacc tttttgcggg ccagcgtaat ctggctcgcc agtggatttt
     4801 acaatggccg gaacatacgg ctactgcact tatccctctt gtcttcacca aatctagcga
     4861 taaaagcgaa gctgcattac ttgccctgcg tttactttac gaacatgggc atggcgaatg
     4921 gctacaaact gtagccaatc gttggcagcg tacagatcta tggcctgccc tggaacaatt
     4981 acttaaacag agtccaatag agatttaccc ggcacgcatt ccaaaagccc ctgatttctg
     5041 gcatccgcaa atgtggtcca ggccgcgcct tatcactaat aatcaacctg ttaccgatga
     5101 cgctctggaa attatcggcg aaatgctgcg ctttacccag gggggacgtt tttatagcgg
     5161 gctggaacaa ctgaaaacgt tctgccagcc acaaacgctg gcagcttttg cctgggatct
     5221 cttcactgcg tggcaacaag ctggtgcccc cgcaaaagac aactgggcat ttctggcgtt
     5281 aagtctcttt ggtgacgaaa gcacggcacg ggatctaacg acacatatcc tcgcctggcc
     5341 acaagaaggc aaatctgccc gtgcggtcag tggcatgaac atccttaccc tgatgaataa
     5401 tgatatggcg ctgatgcagc tgcatcatat atcgcaacgg gcgaaatccc gccccttacg
     5461 tgataacgcg gcggaatttc ttcaggtagt cgcagaaaat cgcgggctaa gccaggaaga
     5521 gttagcggac agattagtcc caacactggg ccttaatgat ccgcaggcgt tgagttttga
     5581 ttttggtccc cggcagttta ccgttcgctt cgatgaaaac ctcaacccgg ttatctttga
     5641 tcagcaaaac gttcgccaga aaagtgttcc ccggttgcgc gccgatgacg atcaactgaa
     5701 agcgcccgag gcactggccc gactaaaagg gttaaaaaaa gatgctactc aggtgagcaa
     5761 aaacctgctc ccgcgtcttg aagctgccct acgtaccacc cgacgctggt cgctggcaga
     5821 ttttcattct ctgtttgtta atcatccctt tacccgtctg gttacccagc gattaatatg
     5881 gggggttatc cggcaaatga accgcgtcgt ttactcaacg cctttcgtgt ggccgcagag
     5941 ggggagttct gcaatgcgca agatgagcca attgacctgc ctgcggacgc tctgattggc
     6001 attgcccacc cgttagaaat gacagcagaa atgcgcagtg aatttgcaca gctttttgcc
     6061 gattacgaaa ttatgccgcc ttttcgccag ttgtcgcgcc gcacggtgct gctcacacct
     6121 gacgagtcaa ccagtaacag cctgactcgc tgggaaggta aatccgctac cgttgggcaa
     6181 cttatgggaa tgcgatacaa aggctgggag tcaggttatg aggacgcatt tgtctatgac
     6241 ctgggtgagt accggctggt ccttaagttt tcacccggtt ttaaccacta caatgttgat
     6301 agcaaagcgc taatgagctt ccgttctctt cgagtgtacc gtgacaataa atccgtcact
     6361 tttgccgaac ttgatgtgtt tgatttgagt gaggcgttaa gcgcacctga cgtcattttc
     6421 cattaaaaca aagcgggcag tgatgcgact gcccgttatc tacacgactt accagcgggg
     6481 aaagcgatga tcgtgcaaaa agagctggtt gctatttacg attatgaggt ccctgtacct
     6541 gaagatccgt tttccttcag acttgagatc cataaatgct ctgaattatt tacaggttcc
     6601 gtctaccgac tggaacggtt ccggctacgt ccaacatttc atcaacgtga tcgagaggat
     6661 gttgacccgc taataaatga tgcgttgatt tatataagag atgagtttat tgatgagcaa
     6721 aaattacgag gcgaatcacc tgaacctgta atagtaattt ttaattgtaa actacagaat
     6781 atattcaacc aacaaataga attatatact ctagataatt caagtcgcag cacatgcaac
     6841 ttgaagtatg acgagtatag ctttattaaa ggggggaacg gtattccccc ttagatgcgt
     6901 ttcaatttgc ccgttacttc aacaaatcaa tcaaccgtaa cagagccact aatagctcca
     6961 gtacagcaat gagcacggtt aacttttgtg tcagcgtgtc catccgctct ccttattaag
     7021 gagcacaaca ccagcacctt acccttacac tgcctgtcga tactggttta cgtttgttga
     7081 gtcgccatgt tgcactccgg atcagagcac tacctgcggc accacccgtc cggtagccag
     7141 gacttgaaat aggttcgatc cggcgcaact tcccaacagt caggattata aatctcatta
     7201 tcttaattgc aacactgtcc agaggtggtt ataattcgtt cgttggaaca cgatgttgca
     7261 caggcagtgg tgttaggcct gaaaatagta aataaaagaa cctgcctcac cagcagtttt
     7321 ttttatttac tgtgatctgc tttccagata tttttcgctc aaacaactaa tgcgccaaac
     7381 atttattgcg cgtaaaatat cgtttatttc attaatacat ttcagggatg aatatatgtc
     7441 accgcagaat aatcatctgc agcgtccgcc tgctgctgtg ttatacgccg atgaactggc
     7501 aaaattaaaa caaaatgata acgcaccttg cccgcccggt tggcagttaa gtttgcctgc
     7561 ggcccgtgct tttatccttg gcgatagcgc gcaaaatatt agccgtaaag tggtgattag
     7621 tccctccgct gtcgaacgta tgttagtcac tcttgccacc ggacgcggtt tgatgttggt
     7681 gggggaacct ggcaccgcaa aatctctcct gtctgaatta ctggcaacct ctattagcgg
     7741 cgatgccggg ttaaccatcc agggcggcgc atctactacc gaagatcaaa tcaagtacgg
     7801 ctggaactac gccctgctta tcaatcatgg tccatcaacg gaagcgctgg tccctgcccc
     7861 gctttaccag ggaatgcgcg acggcaaaat cgtccgtttt gaagagataa cgcgtacacc
     7921 gctggaagtg caagactgtc tgttaggtat gctttctgac agggtgatga cggtgccgga
     7981 actcactggt gaagccagtc agctctatgc gcgagaaggc tttaatatta ttgccactgc
     8041 caatacccgc gatcgcggcg tcaatgaaat gagtgctgcg ctaaagcgtc gcttcgattt
     8101 tgaaactgtc tttccgatta tggatttcgc ccaggagctg gagcttgtcg ccagtgcttc
     8161 ggcgcgtttg ctggcccata gcggtattcc gcataaagtg cctgacgcgg tactggagtt
     8221 actggtccgc accttccgcg atctgcgtgc caacggcgaa aagaaaacgt caatggatac
     8281 actaacagcg attatgtcca ccgctgaagc cgtgaacgtg gcccacgccg taggcgtccg
     8341 cgcctggttt ttagcgaatc gcgcaggtga gccagccgac ctggtggact gcattgccgg
     8401 aaccatcgtc aaagataacg aagaagatcg cgctcgtctg cgccgttact tcgaacagcg
     8461 tgtcgccaca cataaagaag ctcactggca ggcttattat caagcccgcc accgcctgcc
     8521 gtgaggaaag atgcatgagc gagccgttaa ttgtcggcat ccggcatcat agtccggcct
     8581 gcgcccggct ggtgaaatcg ttaatcgaaa gccagcggcc acgatacgtg ttgattgaag
     8641 gccctgctga ttttaatgac cgggtagacg aactgttttt agcccaccag cttccggtag
     8701 ctatttacag ttattgccag tatcaggacg gtgcagcccc cgggcgtggt gcctggacgc
     8761 catttgctga attttcgccg gagtggcagg cgctacaagc cgatcgtcgt attcaggcac
     8821 aaacttactt catcgatttg ccttgctggg cgcagagtga agaagaggac gattcgcctg
     8881 atacgcaaga ggaaagccag accttgctgc tgcgtgccac ccgcatggat aacagcgaca
     8941 ccctgtggga tcacttgttc gaagatgaaa gccagcaaac tgcattaccc tctgcactgg
     9001 cgcactattt tgcccaactg cggggcgact cccccggcga tgctctaaat cgtcagcgcg
     9061 aagcctttat ggcccgctgg attggatggg cgatgcagca aaataatggc gacgtgttag
     9121 ttgtctgcgg tggctggcac gctccggcac tggcaaaaat gtggcgcgaa tgtccgcagg
     9181 taattaacac gccagaattg ccgtcgctgg cagatgccgt tacaggttgt tatctcaccc
     9241 cctacagtga aaagcgcctt gatgtgctgg caggatacct ttcaggaatg cctgccccgg
     9301 tctggcaaaa ctggtgctgg cagtggggct tacagcaggc gggagaacaa ctgctaaaaa
     9361 cggttctcac ccgtttgcgc cagcacaagt tgcctgcttc gaccgcggat atggctgccg
     9421 ctcatctgca tgcgatggca ctggcacagt tgcgcggtca tacactaccg ttacgcactg
     9481 actggctgga tgccatagca ggatcgctga ttaaagaagc cctgaatgcg ccgttgccgt
     9541 ggagctatcg cggcgttatt catcccgata ccgatccgat tctgctaacg ttgatagaca
     9601 cattagcggg tgacggattc ggtaaacttg ccccttctac gccacaaccg cctctgccaa
     9661 aagatgtcac ttgcgaactg gaacgtaccg caatctctct tccggcggag cttaccttaa
     9721 atcgctttaa ccccaatgga ctagcgcaaa gtcaggtgtt acatcggctg gcaatactgg
     9781 agatcccagg gattgtacgc cagcagggaa gcacactgac acttgcaggc aacggtgaag
     9841 aacgctggaa attaacccgc ccgcttagcc agcatgcggc attgattgag gccgcctgct
     9901 ttggtgccac actcctggaa gccgcacgcc ataaattaga agccgatatg ctggacgcgg
     9961 gtggaatcgg cagcattacc acatgtctta gccaagcggc gtcagcgggt ctggcgtcct
    10021 tcagtcaaca attactggag caactcacat tattaatcgc ccaggaaaat caatttgccg
    10081 aaatgggcca ggcgctggaa gtgctatatg ccttatggcg gctggatgaa attagcggta
    10141 tgcaaggcgc gcagatatta caaacgacgt tatgcgcggc tatcgatcgc acgctgtggc
    10201 tgtgtgaatc caacggcaga ccggatgaaa aggagtttca cgctcacttg catagctggc
    10261 aagcgttgtg tcatatcctg cgcgatctac atagcggtgt gcaactcccc ggcgtttcgc
    10321 tttctgcggc ggtagccttg ctggagcgac acagtcaggc gattcatgtc ctggcactgg
    10381 atcgcggcgc gactcttggt gcgctgatgc gtctggaaca tcccaacgct agtgccgaag
    10441 cggcgctgac gatgctggcg cagttatccc cggcacaatc cggcgaggcg ctgcacggtt
    10501 tgctggcatt agcccgtcat caactggcct gtcagccggc atttatcgcc ggtttcagca
    10561 gtcatttaaa tcaactaagt gatgccgatt ttaccaatgc cctgcccgat ttacgcgcgg
    10621 caatggcctg gctaccgcca cgagaacgcg ggacgctggc gcatcaggtg cttgagcatt
    10681 atcaactggc gcaacttccc gtttcggcac tgcaaatgcc gttgcattgt ccaccgcaag
    10741 ccattgcaca tcatcaacaa ctcgaacagc aggcactggc atcgctgcaa cactggggag
    10801 ttttccatgt ctgaactgaa cgatcttctg accacccgtg agctacaacg ctggcgatta
    10861 attcttggcg aagcggcaga aacgacgctt tgtgggctgg atgacaacgc ccggcagata
    10921 gatcacgcgc tggagtggct gtatgggcgc gatcctgaac ggctccagcg tggtgaacgc
    10981 tctagtggat taggtggctc aaatctcacc actcctgagt ggatcaacag tattcacacg
    11041 ctgtttccgc aacaggtgat tgagcggctg gaaagcgatg ccgtactgcg ctacggcatt
    11101 gaagatgtgg tgacaaatct cgacgtgctg gaacgtatgc agccttccga aagcctgcta
    11161 cgcgccgttt tgcacaccaa acatctgatg aaccccgaag tactggctgc cgcccgcagg
    11221 attgtgcgcc aggttgtgga agaaattatg gcgcgactgg caaaggaagt tcgtcaggct
    11281 ttttctggtg tccgcgatcg tcgtcgccgc tcatttattc cactggcgcg aaactttgat
    11341 ttcaaaagta ctctgcgcgc caacctgcaa cactggcacc cgcaacacgg caagttgtat
    11401 atcgaattcc cccgctttaa cagccgcatt aaacgccaaa gcgagcaatg gcaactggtc
    11461 ttactggttg atcaaagcgg atcgatggtc gattcggtga tccactctgc ggtgatggcg
    11521 gcctgtttat ggcagttacc cggcattcgt acccatctgg tggcgtttga cacaagcgtc
    11581 gttgatctca cggcagacgt tgccgatccg gtagagttat taatgaaagt gcaattaggc
    11641 ggcggaacca atatcgccag tgccatggag tatggtcggc aacttattga acaacctgca
    11701 aaaagcgtca ttatcctcgt gagcgatttt tatgaagggg gttcatcatc attgctgacg
    11761 caccaagtga aaaagtgtgt ccagagcggc atcaaagtgc tgggactggc agcgctcgat
    11821 agcacagcaa caccttgcta tgaccgcgat acggcccagg cgctggttaa tgtcggcgca
    11881 caaatagccg ccatgacacc gggcgagctg gcatcatggc ttgcggagaa tcttcagtca
    11941 tgaattcact acgtccggaa ttattagaac tgacaccgca ggcactgacg gcgttaagca
    12001 atgccggttt tgttaagcgc agccttaagg aactggaaaa tggcaacgtc ccggaaatca
    12061 gccatgagaa cagcgcttta atcgccacct tcagtgacgg tgtccgtacc cagctggcga
    12121 acggccaggc actgaaagag gctcagtgca cctatggggc cagcggcatg tgccgtcatc
    12181 gcgtgatgct ggtgttaagt tatcaacgac tttgtgctac cgcccagcct acgggaaaag
    12241 aagaggaatg ggatccggca atctggctgg aagaactggc tacccttccc gatgccaccc
    12301 gcaaacgcgc acaggcgctg gtcgctaaaa gcatcaccat tgagttgttc tgtgcgccgg
    12361 gtgaaattcc ctctgcccgc ttaccgatga gcgatgtgcg tttttattcc cgcagcagta
    12421 ttcgtttcgc ccgttgtgat tgtattgaag gcacactttg cgaacatgtc gtactggcag
    12481 tacaggcctt tgtccaggca aaagcgcagc aagcggaatt gactcattta atctggcaga
    12541 tgcgcagcga acacgtcaca tcatctgacg atccgtttgc cagcgaagaa ggcaaaacgt
    12601 gtcgtcaata tgttcaacaa ctaagccagg cattatggct gagcggcatc agccagccgc
    12661 ttattcacta cgaggccgct ttcagccgcg cgcagcaagc agcggaacgc tgcagctggc
    12721 gatgggtgag tgaatcgcta cggcaactgc gcgccagcgt tgatgccttc cacgcccgcg
    12781 ccagccacta tcatgccgga gaatgcttac gtcagcttgc ggcattaaac agtcgattaa
    12841 attgcgcaca agagatggcc cggagcgaca gtgttggtga agttcctcct gtgccgtggc
    12901 gcacggtcgt tggctctggc attgccggag aagcaaagct tgatcatctg cggctggtgt
    12961 ctttaggtat gcgttgctgg caggatattg agcattatgg tttacgcatc tggtttaccg
    13021 atcccgacac cggcagtatt ttgcaccttt cgcgcagttg gccgcgaagt gaacaggaag
    13081 actcaccggc agctacgcgt cggctgttta gttttcaggc tggcgcactg gcgggtgggc
    13141 aaattgtttc acaagcagca aaacgcagtg ccgatggcga gctgctgtta gctacccgca
    13201 accgcttaag cagcgttgta ccgctgtcgc ctgatgcctg gcaaatgttg agcgcgccgt
    13261 tacgccagcc gggcattgtg gctttgcggg aatatttacg ccagcgtccc cccgcctgca
    13321 tacggcctct taatcaggtc gataacttat ttattctgcc ggtcgctgag tgtatttcgc
    13381 tcggttggga tagcagccgc cagacgctgg atgcgcaggt aatcagcggc gaaggtgaag
    13441 ataatctgct gacgttatca ctgccagcct cagccagcgc gccttatgcc attgaacgca
    13501 tggcggcgct tttgcaacaa acagacgacc ccgtgtgtct ggtttctggc tttgtcagtt
    13561 ttgttgatgg gcaattgaca ctggaaccac aggtaatgat gacaaagact cgtgcctggg
    13621 cgctggacgc agaaactgcg cctgtggtgg tatcactacc ttctgccagc gttctgcctg
    13681 tgccgtctac tgctcatcag ttgctgatgc gctgccaggc gttacttatt caactgctcc
    13741 ataacggctg gcgctatcag gaacagagcg ctattaatca ggcagagttg ctggcgaatg
    13801 acctcaccgc ggtgggtttt tatcggctgg cacatgtgtt ggcacaattt cgtaatacag
    13861 aaagcgaggc acgggtagaa gcaatgaata acggtgtttt gctttgcgaa caattattcc
    13921 ccatgcttca gcaacaagga tgaaatagtg ctttttacta agagttctac tccagttccg
    13981 gactgctcac gccacggtat taggcatatc ctatatagcc cctggtgaga gtcac
//
LOCUS       DAJTUI010000094        13964 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTUI010000094 DAJTUI010000000
VERSION     DAJTUI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.094"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OO464_003152"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OO464_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6228203.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OO464_003153"
     CDS             2219..2767
                     /locus_tag="OO464_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6228204.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OO464_003154"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OO464_003154"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6228205.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO464_003155"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO464_003155"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6228206.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO464_003156"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO464_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6228207.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO464_003157"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO464_003157"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6228208.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="OO464_003158"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="OO464_003158"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6228209.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="OO464_003159"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="OO464_003159"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6228210.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO464_003160"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO464_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6228211.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="OO464_003161"
     CDS             complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="OO464_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR6228212.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3549 a         3563 c         3528 g         3324 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc ataggaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagagtg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcctggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatggt ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgct
//
LOCUS       DAJTUI010000095        13844 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTUI010000095 DAJTUI010000000
VERSION     DAJTUI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.095"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            543..1334
                     /locus_tag="OO464_003162"
     CDS             543..1334
                     /locus_tag="OO464_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228213.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1720..1926
                     /locus_tag="OO464_003163"
     CDS             1720..1926
                     /locus_tag="OO464_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6228214.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            2012..2584
                     /locus_tag="OO464_003164"
     CDS             2012..2584
                     /locus_tag="OO464_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228215.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2586..2870)
                     /locus_tag="OO464_003165"
     CDS             complement(2586..2870)
                     /locus_tag="OO464_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228216.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            2841..2984
                     /locus_tag="OO464_003166"
                     /pseudo
     CDS             2841..2984
                     /locus_tag="OO464_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            4153..5172
                     /locus_tag="OO464_003167"
     CDS             4153..5172
                     /locus_tag="OO464_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6228217.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
     gene            5282..6154
                     /locus_tag="OO464_003168"
     CDS             5282..6154
                     /locus_tag="OO464_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR6228218.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            6488..9610
                     /gene="ag43"
                     /locus_tag="OO464_003169"
     CDS             6488..9610
                     /gene="ag43"
                     /locus_tag="OO464_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR6228219.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVRG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHG
                     SAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            9731..12247
                     /locus_tag="OO464_003170"
     CDS             9731..12247
                     /locus_tag="OO464_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228220.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            12323..12778
                     /locus_tag="OO464_003171"
     CDS             12323..12778
                     /locus_tag="OO464_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228221.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(12897..13108)
                     /locus_tag="OO464_003172"
                     /pseudo
     CDS             complement(12897..13108)
                     /locus_tag="OO464_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13189..>13844
                     /locus_tag="OO464_003173"
     CDS             13189..>13844
                     /locus_tag="OO464_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR6228222.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     T"
BASE COUNT         3718 a         3423 c         3419 g         3284 t
ORIGIN      
        1 ttaacaaaga acattacagt cagtgtgtac attacagatt tatattcagg tgttgcggga
       61 ctacattaca gcagagaggg gtaaggacag agttacaagt actactagag agaatatcac
      121 tgcacagata cttctatgag tatatcttta tgagagatcc gtctacagga ttgggttata
      181 aggtgtattt acaaggtata atcacaggat catttacatg accatacttc tgtaaataat
      241 actaattaac cttaattatt tttttatggt acatgaacta cctgtagctg taacgataaa
      301 caaatcaccc aaaatgaaaa atactggtta tcacagttca gagaactcgt atgagtgtcc
      361 ccctgagcaa tgcttcattt tctgtgattc tgtgtgatag agttatactg agtacagtgg
      421 cagtaacact gctttccatc acttgaggat aaaattcatt aaaaaattaa ttgcatgaat
      481 taacaggttt ttttatgaaa tgttatgcat ctcaaccaat atgattaaca gaaaacttca
      541 tcatgacaac acgtataacg ttcagtatga ccagtgatga gacgctgcga attgtggatg
      601 agtactgtca tacccacaaa ctttcgcgct ctaaggtaat caacgcatta ctcagcgcca
      661 cagcacctgt ccttaatgat attaactgtt attatcaact tgcaggcgaa ctgcagtcac
      721 gcttattaaa tggagtgtat caacgggatc ttcctcgcaa acgcaatgtc gtcagtgcag
      781 agaagtattg tctggaaatc tgggaaaaca aacttttcac cagaagaatc ctggagtttg
      841 acagttcaaa tggtgttctg tacgcgctga aacataaaag acactatcgc cgggataaga
      901 tgattggaag ggttgaaagt cggtgtatca aagacatttg cgaataccag atgcagctat
      961 ccggggagaa gacgaaatat gcctgtttta tttatatcga aagaacaata tacaatcacg
     1021 ataatccatc tgataaaata cctgtaaaag cggctgttgg taatgcagtc atcctgctgg
     1081 caaaggatgt gatatataac gagtactttt ttgatttgcg gcagtcattt tttgtgagcg
     1141 ttacagatct gatggtctct ggcgcaaaag gtattccgga gacacagaca tatccggacg
     1201 tttattgctg gatccctctt ttttccataa attccggcgt gctcattacc cctgtatata
     1261 aaattgaccc tctgaaaccc gtaacggtaa aaaaaccgga taagatcact gttgtttgta
     1321 attacagaga ataatttcag ccttctacct tcaccgcgac agtgtttacc tctgctttat
     1381 cttccgttca gtaaatacaa accaccgctc tggcaggtac agggcgtggt ttatctccga
     1441 tactcccctg tgcagtaata atcatcactg aaaatactga tcccgtcacg gcaaaacgcc
     1501 atcccatatt ccggcaacac aaaatcatct tccgtttata ccggattcac tgacaaaaca
     1561 gacaacctgt attgcctgta cacaacagtt tctctcctgg caggaatatt cccctactca
     1621 ttttttacgt ttctccccgg ttgttatgcc ggtgaaggtg gtgcgtcgtt ttcatcacca
     1681 acgccggttg agattaacaa catccggagg aacattctca tgaatacccc agtttcactg
     1741 atggatgacc agatggtcga catgtcgttt atcactcagc tgaccggcct gaccgataag
     1801 tggttttata aactcatcaa gcatggagcc tttccgcccc ccatcaaact gggccgtagc
     1861 tcccgctggc tgaaaagcga agtggaagcc tggctgcagg cgcgtattgc acagtcccgt
     1921 ccgtaattcc tgaccgttat ccgttccacc gcagcaaagg catgatccgg cccccggagg
     1981 cattttgctg cgcgctaaag gagagaatat tatgcaacaa cttgaaaaca ggtgtgacct
     2041 gctgctcatc cagcatcaga aatggatgac aagcgtgacg aggcttatcg tcgctcatgg
     2101 aatggggtct ccgcatcttc atggttatca ccgtctgacc ctggcacact tttttctgcc
     2161 ggaaaaaggc tccgtcattt cggtggcccc ccagggactc tatcaggtcg tcaacccggg
     2221 aacaccaccg ttcattcctg ccattcagga agggttaatg accagcattc agacccatga
     2281 aatcatgctg cttacacatt ttaatctggg cggcgtttta ctcagtgagc tacatcgtct
     2341 gggtgaaaac cggctggcaa acaggctgaa cagcctgctc cggcgtttcg acgatcgtga
     2401 tctctaccac acgctgatat ggctgtgctg gtatgatctg atgtgtgccc actccatgca
     2461 gccctggact gaagagttaa agcataaatc acatgcagag cttgaaaact gggctgtggc
     2521 gcgcaaacgg gaaaagcgcg agctggaact gatgattgac gagtacctgt tatacgcctg
     2581 ctgattcata acgttcccgg ggttccttcc tcatcgttac acacagccgg acggctatct
     2641 atggctgtcc gccaaatgac catcgccgca tttcgttccg ctgaaaacgg tacgtagtca
     2701 gtgataccgg aatttccgga gagggccgca ggtatattgc ttctgtgcaa ttgatgctta
     2761 tttacattgg atgtagttat ttctgcatcc aatgctatcg gaaagagatt aaccagcaaa
     2821 tggttcctca aatcgtattg atatttaccc ggcgtattga gcaccaccat ctgacactcc
     2881 gttcccgcat caaacgactg gaccgaaaaa cgatttactt ttcacgtgct gtggaaattc
     2941 acgaaaaaat tattgggtca ttcatcgaaa aacatatgtt ctgatgactg gagtcatcct
     3001 ccagaaatat acgtcaactg aacaatatcg attttttatg ggattctata cacttttcca
     3061 tatccctctg aaaatcagtc atcagctcac caacacctct tctgaaactt gtattaaact
     3121 tattgcttaa atagatgata taacatctct tccattgcac tgaatcggcg aaaactgccg
     3181 tttcaataat ctcaacaata ttatttatcg gctaacttta tcagtaaaca atggaatgtc
     3241 acactgatga aatgaacatt cagagtaaga tgataaaatt catataaacg ccattgctga
     3301 atattattta tttcatacag ataaacgata aaatacaaaa gggtatattt ctcaatgtaa
     3361 cagtcaatta ttcatctcca ctggcgagca cggtcagcac acaaacaaag ttagtcctcc
     3421 tgcttaatca ttgcgtatca gaaactaaaa aagaaaaaat tctgcagaca acttaacaat
     3481 cttcatcaca tttcctgaag acagacatat ccacaaacgg tagtcagcgt tgaattaaca
     3541 agtcttacag agctatacgt agcttataaa gccactcaat gttcatcatc acccgactac
     3601 ttatctggaa taccaccagt ataaactcat caataaaaac acctgttgtt aaaataagaa
     3661 tactgtcagc agtaaattat taaaacaaca tctgtccaca ctatctgttc ttttaacgcc
     3721 aacggcagcg aaaatagtgc atcacagcct tcttttttct ttcgccacca ctcagacaaa
     3781 cacaacactc acacccacac cacaaaacag aatcaaaaca taaaattaac aataacagtc
     3841 aaaaggaaaa cacagaatat tgaggtggac tgtcaccagg acagcgcata cgacgcatgt
     3901 gatcagcaca tcccgttctt cgctggcatc cccaaaatgc agaacaaaca atcataacat
     3961 tatgaatata aaagaaaata tgatttttca ccatgaaaaa aacatcaatg tatctctgta
     4021 taaaaataat gataacatga cataaacatt taaaaacata aataaacagc aacaaaaaca
     4081 tttgataatc acttagtgat taaatacaat taagacgatt ttgttataag agaggcacca
     4141 tggagtgaga taatgaataa cgaacagatg cttatcaaat gcagagatcc ccgtaaatgt
     4201 cggttttatg ccgaatatct gaatcacaaa taccctaatg aattccctca ctttgcgcct
     4261 gacaacccac aaaattgtat caggaatgtt aatttatttt taaaggaaaa gtattgtaat
     4321 gtttcacctc ccccctttgg tacttttggt actactgaag aagaagcact caataacgaa
     4381 attaatgagc ttgaaattat tgccgggcag gcaatactgc ctgaagagag ttttctctgg
     4441 ttgaaaactg acgaacttgc gactttcttt acctggacta ctatttatct gtcgaaggaa
     4501 gaccatacaa aattcccgaa aataaaccat aattcatcag gaataaaaaa cggaattgaa
     4561 gtcaggaaaa tacgaacagt taccgatgaa acgttaaaca cttacaaaaa aagtaacctt
     4621 ccggataatc catcatccca taacgaacga ataaaagtaa tcatttcata ttttgactcc
     4681 tgccattctt taattaataa aaatgcaaaa agcatatacc tcgaggaact caggcaggcg
     4741 tggcttaaaa agaaaagcgc ttgtcaaaag tttaagtggc tccttcctga cgatgatgaa
     4801 atgtgtcagt ggttctggac aagactgaaa caaaaacagg atgaaaaaca tccagtacat
     4861 aacccagtga cccggtggtt cataccatct tcaacgaaag aatattatct tgcctctctt
     4921 gtcgcctttg ctttatggaa agaagcgcct gataccattg aactgttcag aacacgaata
     4981 aatcatgcct ggcatcagaa aaaacaaaag gacaaatatc ggagtgatgg taaaaaggcc
     5041 attaatatac atataagaag caatgtgaaa gaaatgcttg atgagctcag aactgtttac
     5101 ggtctgagaa caggagagtt ttttgaaatg ctgattacag agaaatatct tgaacacaag
     5161 cgaaggaagt aattcattac ctgttacatt ctaaataatc tcagacagat attatctccg
     5221 tgaatccccc gccacctttc cggtgcgcgg ggttttgtct tttttcaccg ggaatacatg
     5281 tatgaatccg tctgatgcca ttgaggcaat tgaaaaaccg ctctcctccc tgccttactc
     5341 actttcccgt cacatcctgg aacatctgcg caaactcacc agtcacgaac ccgtgattgg
     5401 cattatgggt aaaagcgggg ccggtaaatc ttcactctgt aatgcactgt ttcaggggga
     5461 ggtcacaccg gtcagtgatg ttcacgccgg cacccgggaa gtgcagcgct tccgtctgag
     5521 tggtcacggt cacagcatgg ttatcactga cctgcccggg gtgggcgaga gccgggacag
     5581 ggatgcagag tatgaagccc tgtaccgtga cattctgcct gaactggacc tggtgctgtg
     5641 gctgattaaa gccgatgacc gggccctgtc tgtggatgag tatttctggc gacacatcct
     5701 gcagtgcgga catcagcagg tgctgtttgt ggtgacgcag gctgacaaaa cggaaccctg
     5761 ccatgaatgg gatatggcag gtattcagcc ttctcccgca caggaacaga acattcgtga
     5821 gaaaacggag gcggtattcc gtctgttccg gcccgtacat ccggttgttg ccgtatcggc
     5881 ccgcaccggc tgggaactgg atacgctggt cagtgcgctc atgacagcgc ttcccgacca
     5941 tgccgccagc cccctgatga cccgactgca ggacgagctg cgcacggagt ctgtccgctc
     6001 tcaggcccgt gaacagttta ccggtgcggt ggaccggata tttgacacag cggagagcgt
     6061 ctgtgttgcc tctgttgtcc gtacggccct gcgcgctgtt cgtgacaccg tggtctctgt
     6121 tgcccgcgcg gtatggaact ggatcttctt ctgaacctgt tgtggatgat gtcctccctg
     6181 cctctgagtc tgctcacaaa agcgctgttt tcgttactgt ctctcttgtc cgtgcaatag
     6241 actgataata gaataaaacg atcgatacct attttaccga tcgttaatat cgatcgttat
     6301 gctaataata actcctgtta gcaacgtgcg cagatacaca cagacatgag attcagggaa
     6361 caacagagcc acacgtcaga aacttccgtc agctggacct ccggcactgt aaccctttac
     6421 ctgccggtat ccacatctgt ggataccggc ttttttattc accctcactc tgattaagga
     6481 aatgctgatg aaacgacatc tgaatacctg ctacaggctg gtatggaatc acattacggg
     6541 cgctttcgtg gttgcctccg aactggcccg cgcacagggt aaacgtggcg gtgtggcggt
     6601 tgcactgtct cttgccgcgg tcacgtcact cccggtgctg gctgctgaca tcgttgtgca
     6661 cccgggtgaa acagtgaatg gcggaacact ggtaaaccat gacaaccagt ttgtatccgg
     6721 aacagctgat ggcgtgactg tcagtaccgg gcttgagctg gggccggaca gtgacgaaaa
     6781 caccggcggg caatggataa aagcgggtgg cacaggcaga aacaccactg tcaccgcaaa
     6841 tggtcgtcag attgtgcagg caggaggaac tgccagtgat acggttattc gtgatggcgg
     6901 agggcagagc cttaacggac tggcggtgaa caccacgctg gataacagag gtgagcagtg
     6961 ggtacacggg ggagggaaag ctgccggtac aattattaac caggatggtt accagaccat
     7021 aaaacatggc ggactggcaa ccggaaccat cgtcaacacc ggtgcagaag gtggtccgga
     7081 gtctgaaaat gtgtccagcg gtcagatggt cggagggacg gctgaatcca ccaccatcaa
     7141 taaaaatggc cggcaggtta tctggtcttc ggggatggca cgggacaccc tcatttacgc
     7201 cggtggtgac cagacggtac acggagaggc acataacacc cgactggagg ggggtaacca
     7261 gtatgtacac aacggtggca cggcaacaga gacgctgata aaccgtgatg gctggcaggt
     7321 gattaaggaa ggaggaactg ccgcgcatac caccatcaac cagaaaggaa agctgcaggt
     7381 gaatgccggc ggtaaagcgt ctgatgtcac ccagaacacg ggcggtgcac tggttaccag
     7441 tacagctgca accgtcaccg gcacaaaccg cctgggagca ttctctgttg tggagggtaa
     7501 agctgataat gtcgtactgg aaaatggcgg acgcctggat gtgctgaccg gacacacagc
     7561 cactaatacc cgcgtggatg atggcggaac gctggatgtc cgcaacggtg gcaccgccac
     7621 caccgtatcc atgggaaatg gcggtgtact gctggccgat tccggtgccg ctgtcagtgg
     7681 tacccggagc gacggaaagg cattcagtat cggaggcggt caggcggatg ccctgatgct
     7741 ggaaaaaggc agttcattca cgctgaacgc cggtgatacg gccacggata ccacggtaaa
     7801 tggcggactg ttcaccgcca ggggcggcac actggcgggc accaccacgc tgaataacgg
     7861 cgccatactt accctttccg ggaagacggt gaacaacgat accctgacca tccgtgaagg
     7921 cgatgcactc ctgcagggag gcgctctcac cggtaacggc agcgtggaaa aatcaggaag
     7981 tggcacactc actgtcagca acaccacact cacccagaaa gccgtcaacc tgaatgaagg
     8041 cacgctgacg ctgaacgaca gtaccgtcac cacggatgtc attgctcagc gcggtacagc
     8101 cctgaagctg accggcagca ctgtgctgaa cggtgccatt gaccccacga atgtcactct
     8161 cgcctctggt gccacctgga atattcccga taacgccacg gtgcagtcgg tggtggatga
     8221 cctcagccat gccggacaga ttcatttcac ctccacccgc acagggaagt tcgtaccggc
     8281 aaccctgaaa gtgaaaaacc tgaacggaca gaatggcacc atcagcctgc gtgtacgccc
     8341 ggatatggca cagaacaatg ctgacagact ggtcattgac ggtggcaggg caaccggaaa
     8401 aaccatcctg aacctggtga acgccggcaa cagtgcgtcg gggctggcga ccagcggtaa
     8461 gggtattcag gtggtggaag ccattaacgg tgccaccacg gaggaagggg cctttatcca
     8521 ggggaataag ctgcaggccg gtgcctttaa ctactccctc aaccgggaca gtgatgagag
     8581 ctggtatctg cgcagtgaaa atgcttatcg tgcagaagtc cccctgtatg cctccatgct
     8641 gacacaggca atggactatg accggattct ggcaggctcc cgcagccatc agaccggtgt
     8701 aagaggtgaa aataacagcg tccgtctcag cattcagggc ggtcatctcg gtcacgataa
     8761 caacggcggt attgcccgtg gagccacgcc ggaaagcagc ggcagctatg gcttcgtccg
     8821 tctggagggt gacctgctca gaacagaggt tgccggtatg tctgtgaccg cgggggtata
     8881 tggtgctgct ggccattctt ccgttgatgt taaggatgat gacggctccc gtgccggcac
     8941 ggtccgggat gatgccggca gcctgggcgg atacctgaat ctgatacaca acgcctccgg
     9001 cctgtgggct gacattgtgg cccagggaac ccgccacagc atgaaagcct catcggacaa
     9061 taacgacttc cgcgtccggg gctggggctg gctgggttcg ctggaaaccg gtctgccctt
     9121 cagtatcact gacaatctga tgctggagcc gcaactgcag tacacctggc agggactctc
     9181 cctggatgac ggccaggata acgccagtta tgtgaagttc gggcatggca gtgcacaaca
     9241 tgtgcgtgcc ggcttccgtc tgggcagcca ccacgatatg aactttggta aaggcacctc
     9301 atcccgtgac accctgcgcg gcagtgccaa acacagtgtg cgtgaactgc cggtgaactg
     9361 gtgggtacag ccttctgtta tccgcacctt cagctcccgg ggggacatga gcatgggtac
     9421 agccgcagcc ggcagtaaca tgacgttctc accgtcacag aatggtacgt cactggacct
     9481 gcaggccgga ctggaagccc gtgtccggga aaatatcacc ctgggcgttc aggccagtta
     9541 tgcccacagc atcaacggca gcagcgctga aggttataac agtcaggcca cactgaatgt
     9601 aaccttctga taattctgca ttgcctctct gtggtcccgg tcatgatgac cgggacccgg
     9661 acgggggaaa acgtttcagt gccacattca ctggcattca caataacatg atattcatca
     9721 cggagtgact atgttacaga tagtcggcgc gctgattctg ctgatcgcag gatttgccat
     9781 tcttcgcctt ttgttcagag cattaaccag cacagcgtct gcgctggcag ggttcatatt
     9841 gctgtgtctg ttcggtccgg ctttactggc tggctatatc actgaacgca taacccggtt
     9901 attccatatt cgctggctgg caggcgtatt tctgacgatt gccggaatga tcatcagctt
     9961 catgtgggga cttgatggta aacatatcgc actggaggct catacttttg actctgtaaa
    10021 atttattctg accaccgctc tcgccgctgg tctgctggct cttcccgtgc agataagaac
    10081 cattcagcag aacgggctca cacctgaaga tatcagcaag gaaattaacg ggtattactg
    10141 ctgtttttat actgcttttt tccttatggc gtgttctgca tacgcaccat tgatcgcatt
    10201 gcagttcgat atttcaccct cactgatgtg gtggggcggg ttgttgtact ggctggctgc
    10261 attagtgacg ctgctatggg cggccagcca gatccaggcg ctgaaaagac tgaccagtgc
    10321 catcagccag acactggaag aacaaccggt gctcaacagt aaatcgtggc tgagcagttt
    10381 gcaaaacgat tacagccttc ctgaaacgct gacggagcgc atctggctga cactcatttc
    10441 acaacggatt tcccggggag aactgaggga atttgaactg gcagacggaa actggttact
    10501 gaacaatgcc tggtatgaaa gaaacatggc agggtttaac gaacagttga aagagaacct
    10561 gtcattcaca cctgatgaac tgaaaacgct cttccggaac cgcctgaatt tatcaccgga
    10621 agcgaatgac gattttctcg atcgttgcct ggacggcggt gactggtacc ccttttcaga
    10681 aggccgccgt tttgtatcat tccaccacgt ggatgagctt cgtatctgtg cctcctgcgg
    10741 gctgacagaa gtacatcatg ccccggaaaa tcataagccg gctccggaat ggtactgctc
    10801 ctctctttgt cacgaaacag aaacactgtg tcaggacatt tatgaacgtt cttacaccgg
    10861 ttttatttcc gatgcaacgg cgaatggtct gattctcatg aaactgccgg aaacctggag
    10921 tacaaatgag aaaatgtttg cttccggagg gcagggacat gggtttgccg ctgaacgggg
    10981 aaaccatatt gtcgacagag tccgtctgaa aaacgcacgg atcctcggtg ataataatgc
    11041 cagaaatgga gcagacagac tggtcagcgg aacagaaatc cagacgaaat attgttcaac
    11101 tgcagcccgt agcgtcggtg cggcattcga cggacaaaac ggacagtatc gttacatggg
    11161 aaatcatggt cccatgcaac tggaagtccc ccgtgatcag tatgccggcg ctgtggaaac
    11221 catgaagaat aagatccgcg aaggtaaagt acccggtgta accgatcccg cagaagcgtc
    11281 ccggctgatt cgtcggggac atctgactta tacccaggcc cgtaatatca cccggttcgg
    11341 gaccatcgaa tcggtcactt atgatattgc cgaggggtcg gttgtcagtc tggcggccgg
    11401 agggatcagt tttgccctga cggcatcggt cttctggctc agcaccggcg atcgcgatgc
    11461 tgccctgcag acagctgctg tccaggcagg aaaaaccttc acccgcacac tggctgtcta
    11521 cgtcacaacc cagcaacttc accggctcac tgttgttcag ggtatgctga agcatattga
    11581 tttttcgacg gccagcccga ctgtccggca ggcgcttcag aaggggaccg gtgcaggaaa
    11641 tatcagtgcc ctgaacaaag tgatgaaggg tacgctggtg acatctctgg cactggtagc
    11701 tgtcacaacc agccctgaca tgatcaaaat gttgcgggga cggatctccg gtgcgcagtt
    11761 catcaggaat cttgccgtgg catcttccgg tgtggcaggt ggtgctgtcg ggtcagtggc
    11821 gggcgggata ttgttcagtc cactgggacc atttggtgca ctgacagggc gtgtggttgg
    11881 cggtgttctg gggggaatga ttgcctccgc tgtatcagga aaaattgccg gagcgctggt
    11941 tgaagaagat cgcgtcaaaa ttctggcaat gattcaggag caggtgacat ggcttgccgg
    12001 cagtttcctg ctgaccggac atgagattga aaatctgaac gcgaatctgg cccgtgttat
    12061 cgatcagaat gctctggaga tcattttcgc cgccggtata caacaacggg ccgcgaccaa
    12121 tatgttaatc aaaccactgg tggtcagtat catcaggcaa cgccccgtca tggaatatga
    12181 tgcatcccat ctcggcaaga tggttaaccg gctggaagaa gcattccccc cggagctccc
    12241 ggcataacgt gtgttctgct ccttttaggt tttatcacct gatttgccag tgtcatcgtc
    12301 atgactcaga gaggtaaata ccatgattca cctgttcaaa acctgcatga ttaccgcctt
    12361 cattctgggg ttaacgtggt ctgccccact ccgggcacag gatcaacgtt acatcagtat
    12421 acgcaataca gatacgatat ggctcccggg aaatatttgt gcttaccagt tccggctgga
    12481 taatggcgga aacggtgaag gatttggccc cctcaccatc actctgcaac tcaaagacaa
    12541 atatggtcag acgctggtga ccagaaaaat ggaaacggaa gcttttggtg acagtaatgc
    12601 cacgcgaacc acagacgcat ttctggaaac ggagtgcgtg gaaaatgtcg ccacaaccga
    12661 aatcattaaa gcaactgaag aaagtaacgg ccatcgtgtc agtctgccgt tatcggtttt
    12721 cgatccccag gactaccatc cactgctgat taccgtttcc ggaaaaaatg ttaactgatc
    12781 cctgaagtca gtttcatgct tcagcctctg ccacaacgta tggcagaggc tatttatcag
    12841 gagaaaccag atatgccggg ttacacacag agtatgtcct ggctgaagga tctttctcat
    12901 acgggcaggc ggttgctgtc ataaccgctt accgcaatgt gtttatccag gatgacccgg
    12961 gaatgcattt ccgtcgggtt atccgcaacg cggaaggtca gcgacgctga cgcagccgga
    13021 actctgaagc tgatgccgga aaacagctta atgcgtggct cgccagtgga gggcttctca
    13081 gacaataaac ttcattgatt tcatccatca ggccgcgtct tctccgggag acgcggcttt
    13141 tttcatttgt tgcaccagta aatcttaacc accgataagg agcaaagtat gcaattagtc
    13201 agtcgttttg gttatgcgaa ccagatacgc cgtgaccgtc cgctgacaca tgaagagctg
    13261 atgcaccatg tgccggggat tttcggggaa gagaaacaca cgtcccgaag ccagaactat
    13321 acgtacatcc ccaccatcac cgtactggaa agtctgcagc gggaaggctt ccagcccttt
    13381 ttcgcctgcc agacccgtgt gcgcgacccg ggccgccggg gatacacaaa acacatgctg
    13441 cgtctgcggc gggccggaga gataaacgga gaacatgtcc ctgaaattat tctgctcaac
    13501 tcccatgacg gtacctccag ctaccagatg ctgccgggtt acttcagatt cgtctgccag
    13561 aacgggtgtg tctgtggtca gtctctgggg gaagtgcgtg ttccgcaccg gggaaacgtg
    13621 gtggagaaag ttattgaagg ggcttacgaa gtggtgggcg tgtttgaccg gattgaggag
    13681 aaacgtgatg ccatgcagtc gctggtcctg ccgccaccgg cacgccaggc gctggcacag
    13741 gcggcactga cttaccgtta tggtgacgaa catcagcccg tcaccaccgc cgacattctg
    13801 acgccacgac gccgggagga ttacggtaag gacctgtgga gtac
//
LOCUS       DAJTUI010000096        13248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTUI010000096 DAJTUI010000000
VERSION     DAJTUI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.096"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(16..998)
                     /gene="kdgT"
                     /locus_tag="OO464_003174"
                     /pseudo
     CDS             complement(16..998)
                     /gene="kdgT"
                     /locus_tag="OO464_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="OO464_003175"
     CDS             complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="OO464_003175"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6228223.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2163..3197
                     /gene="rhaT"
                     /locus_tag="OO464_003176"
                     /pseudo
     CDS             2163..3197
                     /gene="rhaT"
                     /locus_tag="OO464_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3194..4060)
                     /gene="rhaR"
                     /locus_tag="OO464_003177"
     CDS             complement(3194..4060)
                     /gene="rhaR"
                     /locus_tag="OO464_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR6228224.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="OO464_003178"
     CDS             complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="OO464_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR6228225.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5240..6709
                     /gene="rhaB"
                     /locus_tag="OO464_003179"
     CDS             5240..6709
                     /gene="rhaB"
                     /locus_tag="OO464_003179"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6228226.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6706..7965
                     /gene="rhaA"
                     /locus_tag="OO464_003180"
     CDS             6706..7965
                     /gene="rhaA"
                     /locus_tag="OO464_003180"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR6228227.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8207..9031
                     /gene="rhaD"
                     /locus_tag="OO464_003181"
                     /pseudo
     CDS             8207..9031
                     /gene="rhaD"
                     /locus_tag="OO464_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9041..9355
                     /gene="rhaM"
                     /locus_tag="OO464_003182"
     CDS             9041..9355
                     /gene="rhaM"
                     /locus_tag="OO464_003182"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR6228228.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9656..10102
                     /locus_tag="OO464_003183"
     CDS             9656..10102
                     /locus_tag="OO464_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR6228229.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10113..11564
                     /locus_tag="OO464_003184"
     CDS             10113..11564
                     /locus_tag="OO464_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR6228230.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11554..12624
                     /locus_tag="OO464_003185"
     CDS             11554..12624
                     /locus_tag="OO464_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6228231.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12624..>13248
                     /locus_tag="OO464_003186"
     CDS             12624..>13248
                     /locus_tag="OO464_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR6228232.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3158 a         3458 c         3491 g         3141 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc cagcttcttc tttcgtaccg
      601 tactgctgca tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc
      661 gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc
      721 gaggcaattg ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat
      781 ttacgcagta ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac
      841 accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc
      901 cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc
      961 ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt
     1021 tttaagggct gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga
     1081 cattcattta atcaataaaa gtgatcaaaa tcacatcaat tgaaacgctg ttttattttt
     1141 ataggaccat tgtttttatt ttggaattga tcacaaaaaa accaccgccg ttggcgatgg
     1201 ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg caaatgatta
     1261 ttttttcgcc gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt
     1321 gatgtagtcc ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag
     1381 gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg
     1441 gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga
     1501 accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa
     1561 gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt
     1621 ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc
     1681 tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg
     1741 caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg
     1801 gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga
     1861 tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc
     1921 gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat caatgccgta
     1981 cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact
     2041 taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag
     2101 cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga
     2161 agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag
     2221 cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag
     2281 tcggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg
     2341 atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg
     2401 gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt
     2461 cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa
     2521 ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg
     2581 gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc
     2641 gcaagatggg cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga
     2701 tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg
     2761 aagccgctgc cgcacttggc gtcgatccac tgtatgttgc tctgccaagc tatgttgtca
     2821 tcatgggcgg cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga
     2881 aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt
     2941 tactctcggc actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc
     3001 acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg
     3061 tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc
     3121 cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca
     3181 tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc
     3241 cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg
     3301 aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga
     3361 cctgccgcag atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa
     3421 cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc
     3481 tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg
     3541 aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag
     3601 ccatttcgtt agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct
     3661 gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc
     3721 taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa
     3781 taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa
     3841 tataaaagag atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat
     3901 taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt
     3961 gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga
     4021 gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg
     4081 caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc
     4141 tggcgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta
     4201 ctgtcgctga atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagt
     4261 agatgtcggg ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt
     4321 tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca
     4381 tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt
     4441 gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc
     4501 ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc
     4561 atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac
     4621 ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat
     4681 cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga
     4741 tgccgatcat gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca
     4801 ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga
     4861 tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac
     4921 ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg tcaacacggc
     4981 gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa
     5041 aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac
     5101 cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
     5161 ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga
     5221 aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc
     5281 cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga
     5341 aatccatcgt tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga
     5401 tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat
     5461 cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca
     5521 gcgtgtgggc ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc
     5581 acaacagcaa ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt
     5641 caatacgctt tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat
     5701 tgctcacgct ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg
     5761 ggaatatacc aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga
     5821 gtcgctactg gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg
     5881 taatgtcatt gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc
     5941 cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta
     6001 tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga
     6061 cacggcgctg gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct
     6121 gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga
     6181 tctcccggcg cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc
     6241 caatgacgat cgctttatta atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg
     6301 ggaaacggcg caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag
     6361 tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt
     6421 ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc
     6481 cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat
     6541 cggcatcgag ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt
     6601 cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta
     6661 tgtggcgcag attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg
     6721 gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag
     6781 gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt
     6841 tccggttttg aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat
     6901 ccgggcaaag cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg
     6961 attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc
     7021 tcgcgcgacc agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat
     7081 cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt
     7141 acgctctcgc atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc
     7201 cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg
     7261 atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg
     7321 gcagcactgg atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt
     7381 gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac
     7441 atggggtatg ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg
     7501 actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtaccgca gttgctgctg
     7561 cacgtcagcc gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc
     7621 caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt
     7681 gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg
     7741 aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg
     7801 gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgcggcag
     7861 gcggtctggg aaatgtattg ccaacatcac gatacgccag caggtagcga atggctggag
     7921 agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg
     7981 gcacgagcgc cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc
     8041 ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa
     8101 caccgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc
     8161 cgcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac
     8221 ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctagc tgaaaggctg
     8281 ggacgagcgc aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta
     8341 tcacgacaac ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact
     8401 ggcaaatacg ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga
     8461 tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct
     8521 ctgggggtta accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca
     8581 ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac
     8641 caacctgatc gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact
     8701 gtgggaaggc agcaccgaat gtctggtagt attcccggat ggcgttggca ttctgccgtg
     8761 gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc
     8821 gctggtgttg tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt
     8881 tggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg
     8941 cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt
     9001 tacaccacta gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt
     9061 catgcaggta aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc
     9121 ggaactggaa gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa
     9181 agcgcgtaat ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt
     9241 agccagtact gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa
     9301 cccagataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc
     9361 caatgtgccg gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat
     9421 attacatctc ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat
     9481 gaataaccca ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc
     9541 aaatctgacc tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc
     9601 acttaatcgc tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc
     9661 agctcttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct
     9721 gaagcagttg gcgacaatag cgttacaaaa cggttttatc accgactcac accagttcct
     9781 gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga tttggttccg gtgtcgcagt
     9841 gccgcacggt aaaagcgcct gcgttaaaca accgttcgta ttattcgccc gcaaagcgca
     9901 ggctattgac tggaaagcca gcgatggcga agacgtcaat tgctggatct gcctcggcgt
     9961 gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc gcaaaattat
    10021 tcaccaggaa tttattcatc aactgcaaca gggcgatacc gaccaggtgc tggccttgtt
    10081 aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc atccttacgt attgtcgcga
    10141 tcaccaactg ccccgccggg atcgctcaca cctacatggt ggcggaagcc ctggaacaga
    10201 aagcccgttc tctcggccat accataaaag tggaaactca agggtccagt ggcgttgaaa
    10261 accgcttatc cagcgaagag attgccgctg ccgattacgt cattcttgct accgggcgtg
    10321 gcctgagcgg tgatgatcgc gcgcggtttg ccgggaagaa agtttatgag attgccatct
    10381 ccctggcgtt gaaaaatatc gaccagattt tcagcgaatt accgacaaac tcgcagcttt
    10441 ttgccgcaga tagcggcgtg aagcttggca aacaggaagt gcaaagcggc agcgtgatga
    10501 gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggt ggtatcctgg
    10561 tggcgcttgc caacatgctg gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg
    10621 cgccgtcgtt tacctgggta gttgaatcca tcggttacct tggcttcacc tttatgatcc
    10681 ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc gctccggcgt
    10741 ttctggtttg ctatctggcg aacgacaaag cgctgctcgg cacccagtcg ggtgcgggat
    10801 tcctcggcgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg ttccgcaaag
    10861 tgcgccttgg caaagcatta caaccgctgc tcggttcaat gctgatcccg ttcgttactc
    10921 tgctggtttt cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc gacctcatgg
    10981 gcgggctgct ccacttcctg aataccattc ctccatcaat gaagtttgcg gcagcgtttc
    11041 tggtcggtgc gatgctggcg ttcgatatgg gcggccccat caataaaacc gcctggttct
    11101 tctgcttctc actgctggaa aaacacattt atgactggta cgccatcgtc ggcgttgtcg
    11161 cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc
    11221 gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac
    11281 cggctatacc ttacgctctg gcagctccgt tgccgatgat taccgccaat acgctggcgg
    11341 gcggcattac cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca cctggtttgg
    11401 gtatctttga cccgctgatc ggcctgatgt cgccggtagg ttcgttttat ctggtgctgg
    11461 cgattggcct cgcgctgaac atttcattca ttatcgtcct gaaaggattg tggttacgcc
    11521 gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca ttgagttact gcaacagttg
    11581 tgcgaagcca gcgccgttag cggcgatgaa caggaagttc gcgacatcct gataaacacg
    11641 ctggaacctt gcgttaatga aatcaccttt gatggtctgg gcagctttgt tgcccgtaag
    11701 ggaaataaag gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg ctttatggtc
    11761 acccacatcg acgagagcgg ttttctgcgt ttcaccacca ttggtggctg gtggaatcag
    11821 tcgatgctca accatcgggt aaccatacgc acacacaagg gagtgaaaat ccctggtgtg
    11881 attggtttcg tcgcgcctca tgcgttaacg gaaaagcaaa ggcaacagcc gctgtcattt
    11941 gatgagatgt tcattgatat tggcgcaaac agtcgcgatg aggtggaaaa acgcggtgtg
    12001 gcgatgggcg attttattag cccggaagcc aattttgcct gctggggcga agataaagtg
    12061 gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga tggctgaact attgcagacg
    12121 gtgaataatc ccgaaattac gctgtatggc gtcggcagcg tggaagaaga agttgggcta
    12181 cgcggggcac aaacctcggc ggaacacatt aaaccggatg tagtgatcgt gctggatacc
    12241 gctgtcgcag gcgatgttcc gggcattgat aacattaaat acccgctgaa actgggccag
    12301 gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact ggtagcagcg
    12361 ttaaaaaact gtgccgcaca taacgattta ccgctgcaat tttccaccat gaaaaccgga
    12421 gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt tgcgctgtgc
    12481 ctgccaaccc gctatctgca cgctaacagc ggcatgattt caaaagccga ttacgatgct
    12541 ctgctcacgt tgatacgggg ttttctgacg accttaaccg cggagaaagt caacgcgttt
    12601 agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc
    12661 tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca gactggcgtt tcaggcagga
    12721 ccatcctgcg tgatattgac tatctcaact ttacccttaa cggcaaagcc cgcattttcg
    12781 ccagtggcag tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc
    12841 tgcaaaagca cgataacgac gatcggctgc tggcgctgtt attactgaat actttcactc
    12901 cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac gtgggtagca gagcgtctgc
    12961 cccagttaaa acagcgttat gaacgcactt gttgcctggc cagccgccct ggtttgggcc
    13021 atttcattga tgagacagaa gagaaactcg ttatcttgct ggcgaacttg ctgcgcaaag
    13081 atccgttttt aattccgctg gcgggcataa cacgagacaa ccttcagcat ttatccacgg
    13141 cctgcgacaa ccaacaccgc tggccgctca tacagggtga ttatctctcc agcctgattc
    13201 tggcgattta cgccctgcgt aatcaactga ccgatgagtg gccgcaat
//
LOCUS       DAJTUI010000097        13086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTUI010000097 DAJTUI010000000
VERSION     DAJTUI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.097"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..890)
                     /locus_tag="OO464_003187"
     CDS             complement(<1..890)
                     /locus_tag="OO464_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6228233.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1934
                     /locus_tag="OO464_003188"
     CDS             1131..1934
                     /locus_tag="OO464_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6228234.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLLVTRASDHNPLLVEFSPGKPDK"
     gene            2012..2782
                     /locus_tag="OO464_003189"
     CDS             2012..2782
                     /locus_tag="OO464_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6228235.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2830..4176)
                     /gene="mltD"
                     /locus_tag="OO464_003190"
     CDS             complement(2830..4176)
                     /gene="mltD"
                     /locus_tag="OO464_003190"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6228236.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4248..5003)
                     /gene="gloB"
                     /locus_tag="OO464_003191"
     CDS             complement(4248..5003)
                     /gene="gloB"
                     /locus_tag="OO464_003191"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6228237.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5037..5759
                     /locus_tag="OO464_003192"
     CDS             5037..5759
                     /locus_tag="OO464_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6228238.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5756..6223)
                     /gene="rnhA"
                     /locus_tag="OO464_003193"
     CDS             complement(5756..6223)
                     /gene="rnhA"
                     /locus_tag="OO464_003193"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6228239.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6288..7019
                     /gene="dnaQ"
                     /locus_tag="OO464_003194"
     CDS             6288..7019
                     /gene="dnaQ"
                     /locus_tag="OO464_003194"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6228240.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7152..7228
                     /locus_tag="OO464_003195"
     tRNA            7152..7228
                     /locus_tag="OO464_003195"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7186..7188,aa:Asp,seq:gtc)
     gene            complement(7359..8405)
                     /locus_tag="OO464_003196"
     CDS             complement(7359..8405)
                     /locus_tag="OO464_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR6228241.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8419..8978)
                     /gene="tssG"
                     /locus_tag="OO464_003197"
                     /pseudo
     CDS             complement(8419..8978)
                     /gene="tssG"
                     /locus_tag="OO464_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8975..10858)
                     /gene="tssF"
                     /locus_tag="OO464_003198"
     CDS             complement(8975..10858)
                     /gene="tssF"
                     /locus_tag="OO464_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR6228242.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10874..11368)
                     /gene="tssE"
                     /locus_tag="OO464_003199"
     CDS             complement(10874..11368)
                     /gene="tssE"
                     /locus_tag="OO464_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR6228243.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11365..12195)
                     /locus_tag="OO464_003200"
                     /pseudo
     CDS             complement(11365..12195)
                     /locus_tag="OO464_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12182..13084)
                     /gene="tagK"
                     /locus_tag="OO464_003201"
                     /pseudo
     CDS             complement(12182..13084)
                     /gene="tagK"
                     /locus_tag="OO464_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596542.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3007 a         3392 c         3480 g         3207 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattattggt tacgcgcgct tccgatcaca atccgctact cgttgaattc agtcccggca
     1921 agcctgataa ataaggtatg tcaggtctgc cacagggcag accaacgttt ggcgctgtcc
     1981 ttttatactc aaacaacaaa aggacagcgc aatgacaaca caatcccacc atgaccacgt
     2041 agaaaagcag tttagctctc aagcctgcga atatctgacc agtaccgtac atgcatccgg
     2101 acgagattta cagcgcctgg cggtgcgtct ggctgattat cctggtgcaa gtgcacttga
     2161 tatgggctgc ggagcagggc atgccagctt tgttgctgcg caaaacgtga gcgcggtggt
     2221 ggcgtatgac ttatctgccc aaatgctgga tgtcgtggca caagctgccg aagcccggca
     2281 actgaaaaat atcaccaccc gccagggata tgccgaaagt ctgccatttg ccgataacgc
     2341 atttgatatt gttatcagcc gttattctgc ccatcactgg catgatgttg gtgcagcact
     2401 gcgtgaagtg aaccggatat tgaaacctgg cggtaggatg attgtgatgg acgtaatgtc
     2461 tccgggtcat ccagtgcgcg acatctggtt acagacggta gaagcattac gcgatacctc
     2521 tcacgtacga aactacgcca gcggtgagtg gttgaggtta atcaatgaag ccaatctgat
     2581 agttgataat ttaattacag ataagttacc gctggaattt tgttcatggg tcgcgagaat
     2641 gcgtacgcca gaagcgttag tagacgctat tcgcatttac caacagagcg catcgacaga
     2701 ggtgagaacg tattttgcct tgcagaatga cggctctttc accagtgata tcatcatggt
     2761 agaagcacat aaagcggcat aaataaaaaa ggcaccgggg gaatcggtac ctttttatta
     2821 tctggtttgt caggaatctg gcatgttgtt gtttttcaca aacaacgtca gcttgtcgcc
     2881 tggttgcaga ttcgcagtgt cgctgttcca gcgcatcaca tctttgatgt tcacgccgtg
     2941 gcgtttagca atgcttgaaa gcgaatcgcc tttgcgcaca cgatacgtaa tgctatcgct
     3001 gttgtttgcc agtcgctgtg caccaatcgt caaactttgg cctggcttca gcttagatcc
     3061 gcgcagtttg ttccactgct gcaaatcttt ggtgcttacg ccgagacgtg aagcgatgct
     3121 tgaaagcgtg tcgccagagc gtacggtgta aacacggctg ttaagcggcg tattgttggc
     3181 aaccagcgtc gactgtacag cagcaatttc gcctgaagcg agagattcac gcagttgatc
     3241 tgcatgcttc tttggcacca tcacgtactg cggcccactt gcgcccagcg tggagccttt
     3301 cacgccagcg ttgaatgtct tcagcttgct gacggaaatc cccgccatat ctgcaacctt
     3361 cgccatttca accgggctac tcaggtgcac acgcgccaga gcacggcttt catcggtcgt
     3421 tggcagacgt acgccataac gcttgctgtt tttgagaata tcactcaatg ccagcatttt
     3481 aggcacgtac tgcttcgttt cctgcggcaa cggtaacgac cagaagtccg tggatttccc
     3541 acgcgctttg ttcgttttaa ttgccttcat gacccgacct tcgccgctgt tataagccgc
     3601 tacggtcaga agccagtcgt cgtcaaacat tttgttcaga cgctgcatca tattcagcgc
     3661 ggcagttgtt gaagcaacaa catcgcgacg cgcgtcataa ttgcgggtct gtttcaaacc
     3721 ataattgcgc cccgtgctcg gaatgatctg ccagatgcct gcggcattgg cgccagacgt
     3781 tgcgtgagga tcaaaagcgc tctccactat gggtagtagt accagttcca taggcatgtt
     3841 acgtttttta acttgccctg ctatccagta catatacggc tctgcccgta aagttacatc
     3901 gtggagatag ctcttattgc gtaaatattt ctgtttctgt tcgcgaatcc ggtcattttc
     3961 cggaattccc atctttagct cgtcgccaat gaaagcccac aagtcaccat ctggcgcgat
     4021 agacgtccca tcgtccatcc atcgtgcctg acttgtaaac tttgctgctt ccccttgacc
     4081 agctgcagaa aggctctgtg cgtgctgttg aacgttgccg gtactctggc aacccacgag
     4141 caggacagag gcgagtaata tcgcttttgc cttcatgtgt gtgtcaatag ttgcttaaaa
     4201 gacgaccgat cataacggcg aacggagccg atgacaagaa agttttatca gaacctatct
     4261 ttctttgacc ttaaccatgc aaaacgctct tcaggttgtt gcaataatgt ttcttcatta
     4321 attccattaa ttaaatcaat atcttccgtt cttaaaaaaa cattaatttg ccgctcattt
     4381 ttcagaatta cgggtagtgt tatttgattt tttgcccgta actccttaac tttacgataa
     4441 taatcattta tggacaaatc gtgcggaaaa atactcaaag caaacttcat atttgataac
     4501 gtatattcat gagcacaaca taccaatgta tcgtcaggta acgcacttag ctttttaagt
     4561 gattgataca tttgtaaagc tgtcccttca aacaaccgac cacacccacc agaaaacagc
     4621 gtgtcgccgc aaaatagata aggtttactg aagtaacaga tatgtcctaa agtgtgaccc
     4681 ggtgtggtaa ttacactaaa ttcatgccct aaaacgaagg cagtttcgcc atctttgact
     4741 acctgtgttg ttcccttatc ttgtgtctct tgtggaccat acactacaat ttgcggaaac
     4801 ttttccacca gttcttttac gccgccaacg tgatcgtggt gatggtgggt tagaaatatg
     4861 gcctccggtt gccagttatt ggcggtaatg gcgtttaata ctggctctgc gtctccggga
     4921 tcgacaatca ggcagcgacc tgcctcatca ttcaaaaccc agatgtaatt gtcatcaaag
     4981 gcgggaatac tgttaagatt catagattac ctctcagtgt aaaacggaag gttgtgatga
     5041 aaccggcaag agtccctcaa actgtcgtgg ctcctgattg ctggggcgat ttgccctggg
     5101 gagagcttta tcgcaaggcg ctggagcgcc agctcaaccc gtggttcact aaaatgtatg
     5161 gttttcatct gcttaagatt ggcaatttaa gcgcagaaat caattgcgaa gcgtgcgcgg
     5221 tttctcatca agtgaatgtt tctgcgcaag gaatgcccgt ccaggtgcag gcggatccac
     5281 ttcatcttcc ttttgccgat aaatccgttg atatttgtct actggcacat accttgccgt
     5341 ggtgcaccga tccgcatcgt ttattacgtg aagccgatcg ggtactgatt gatgatggct
     5401 ggctggtgat tagtggcttc aaccccatca gtttaatggg attacgcaaa cttgtgccgg
     5461 tattgcgcaa aacctcgccc tataatagcc ggatgtttac tctgatgcgg caactggact
     5521 ggctctcttt gttgaatttt gaagtgctgc acgccagccg tttccacgtt ctcccgtgga
     5581 acaaacacgg aggaaaacta ttgaatgcgc atattcctgc gcttggttgc ttacaactta
     5641 ttgttgcccg gaaacggact attcctttaa cgctaaatcc gatgaaacag agtaaaaaca
     5701 agccacgaat tcgccaggcg gttggagcca cccggcaatg tcgtaaacca caggcttaaa
     5761 cttcgacttg gtagcccgta tcttccagtg tgggattcat cgccgcggca cgagccagtt
     5821 catcacagcg ttcgttttcc gggtgtccgg catggccttt aacccattcc catttgattt
     5881 gatgctgccc caatgcagca tcaagacgtt gccagagatc gacatttttt actggttttt
     5941 tgtctgctgt tttccagcca cgttttttcc agttatggat ccactgggtg ataccctggc
     6001 ggacatactg gctgtcggta ctcaaaatga cttcgcaatg ttcttttaac gcctccagcg
     6061 cgacaatagc ggccatcaac tccatacggt tgttggtggt gcgggtgtag cctgcgctaa
     6121 aggttttctc gcgtccgcga tagcgtaaaa tagcgccgta acccccaggt ccaggattac
     6181 ccagacacga accatcggtg aaaatttcta cctgtttaag catctctggt agacttcctg
     6241 taattgaatc gaactgtaaa acgacaagtc tgacataaat gaccgctatg agcactgcaa
     6301 ttacacgcca gatcgttctc gataccgaaa ccaccggtat gaaccagatt ggtgcacact
     6361 atgaaggcca caagatcatt gagattggtg ctgttgaagt agtgaaccgt cgcctgacgg
     6421 gcaataactt ccatgtttat ctcaaacccg atcggctggt ggatccggaa gcctttggcg
     6481 tacatggtat tgctgatgaa tttttgctcg ataagcccac gtttgccgaa gtagccgatg
     6541 agtttatgga ctatatccgc ggcgcggagt tggtgatcca taacgcagcg ttcgatatag
     6601 gctttatgga ctacgagttt tcgttgctta agcgcgatat tccgaagacc aataccttct
     6661 gtaaggtcac cgatagcctt gcggtggcga ggaaaatgtt tcccggtaaa cgcaacagcc
     6721 tcgatgcgct atgtgcccgc tacgaaatag ataacagtaa acgaacgctg cacggggcat
     6781 tactcgatgc ccagatcctt gcggaagttt atctggcgat gaccggtggt caaacgtcga
     6841 tggcttttgc gatggaagga gagacacagc agcaacaagg tgaagcaaca attcagcgca
     6901 ttgtacgtca ggcaagtaag ttacgcgttg tttttgcgac agatgaagag ctggcagcac
     6961 atgaagcccg tctcgatctg gtgcagaaaa aaggcggaag ctgcctctgg cgggcataaa
     7021 tacatgtaaa aggcgctaaa aatagcgact tgggcgattt ttgcagcaaa cgattcaaaa
     7081 gatgagaaaa atcgttgacg aaggtcgagg caatccgtaa tattcgcctc gttcccaacg
     7141 gaacacaacg cggagcggta gttcagtcgg ttagaatacc tgcctgtcac gcagggggtc
     7201 gcgggttcga gtcccgtccg ttccgccaat attcagaaag cccgaatcag taatgattcg
     7261 ggctttcgtc gttttggtga agtgagaatt tttcactccg aagtaaaacg gatatgattt
     7321 aaaaaatggc cagacattat aaattctggc cacttctgtt actccagcgc atcccatttc
     7381 tccagcagtt ctactggaat ttcatttatt acctccgaga cgcgttttcc gaccaggcgt
     7441 tctgcctggc gcagcaaagg aatggtcggg ctgctcggtt cgctgtgctc aaaccagcga
     7501 cggatgacac gcaggcgttc cagcgcgtcg tttcggtcgc ggatctgccg gggttctgcg
     7561 ccggtcatcg gtgatatttc ctccgcgata tgcaccggta gcgcaatctg ctctgcctgc
     7621 atctgttcag tatgaacgat ggtggcgttt tccggctgct cagcctgtgg ttgtggcagg
     7681 atctccggcg tggtgctctg cactgctccc ggtaacaggg ccagcagttg cgtcagacgg
     7741 gagaagtccg gtgcctggtc attcagcgtt tcacgcgcat ggcgttgcag ccgctcagtg
     7801 gtctctgctg cctggcgaaa ggcatccagc ggcagtgtgc cgcgcgcttc caggtctgcc
     7861 agttgctggc gtaccgattc tggtgccagc gcatccgccg ggcgtaatgc agatagcgaa
     7921 cgctccacat cccgcacctg aaggcgcatg gcagcattat ttgacagtgt aatgccacga
     7981 atatccgcca ttacaccttc gtggtccagc aacgccgcca gcgcgttact gcgtgccacc
     8041 gcagcatctt cggcggtgat atcttcagtc gcgagcagtt gaggatggat ggcgtccggg
     8101 tacgtggagc acaacgtttc cagcagcgtg agcgcctccg acagtcccct ggcaccggcc
     8161 tgctggatac ggcagcgcag caggacaacc agaatgcgga tatctttgct gcgcatcagc
     8221 agacgatggg cgtcccgctc aagctccgcc cagttaatgt tttccggcgt actgacaaaa
     8281 tcgccatatt gcgcttcttc acgcggtgcg gcgcgggtga acagcagcag gtattccgga
     8341 tcgtactcca gatccgggcc gcaggggcat tccgccgaaa ttgggtcaga caggaatatt
     8401 tccatttaat tatcctcagt gcgccagcgt ggtggccaga ccgtccggat tgctggactc
     8461 acgcaccaga tgggccgatg ccgccagacg agcgtgatct ggcaacgggc tgtcagcgct
     8521 ctcccgcaaa tcctgcccgc tgaggctggc gacataaaag gcaaagcggt catgttgcgg
     8581 tttatccaga ccgcccccag cggactgggc gctgcgccat gcctggtaaa actgcgtcag
     8641 ccagcggtga tgaaaaagat cagaaaaacg ggtcagcgtc ggctttcagt ccggttacgc
     8701 gccagttcgg tgtagtgcag cggcagtgga ccgttcggcc cccacagcac gaggctgtac
     8761 agactgatgt gcaggcgccc ttcctgccag cccacactgg cgatttctcg tggcgcaaag
     8821 gtcagcgcgg gcgtttgtcc gaggcgaaat ttttccatcc gtggttgcca ggtgttgccc
     8881 gccggaacgg tacacaactg cacatccacg cggcgcatca ggttcagaaa accgtaacgc
     8941 cagggggggg cctgcgcctg agtgaaatcg ttgctcatac gcttccccgc tgtcccgccc
     9001 tgacaggcca ggtcataatt ttgccgcgct gcatgctgtg cagcgtcatc tgcgagaagg
     9061 tattaatgga aacatggcgg gcgatatagt gttccagcac caggccgaac agatacggac
     9121 tgatgccgga aaaaccttct tcatccaccg tcagttcaca actgacgcca cgcccataga
     9181 ccagtagtcc ggaaccgggc agacggcggg tgaccggggt cgttttacaa ccaatcaggc
     9241 tgcgcacctg gcgcgattgc ggactgtcat gagcaggaat aaacaggttc agcagatctc
     9301 gcaacgcctg gccgccggta cggtgatcca gatcggccag cggcagataa ttaaaagata
     9361 actggcggat caaccgccat gccatctcac gttcagccat cgggggctgc ggcgagcgtg
     9421 gcgggcggat aagccctacg cccgccaccg ggatggctgc atccacggtt agatcgtccc
     9481 ggccattgcg ggcaataaga cagggcagat cgcggttagt gaccatggcc gtgatggtga
     9541 tgtggcgcag attttccggg tacggcgctt catactggtc aaccagcgac aggaacactt
     9601 ccgagcccgt atagggggta cgggtgccgt aacggcgggc gttttctgat aagcggcggg
     9661 gctcccggcg cagggaaaaa taacgtccgt gattgccttc atcattattg cgggtgtggt
     9721 agagcgggcg aaaggccatc ttccgtgtgg tgtcggtttc cagcccttcc acttcctgga
     9781 ccgagaacac ctcataatcc agcggatggg ttctgtccac caccagatgc tgttccgtcg
     9841 tgctgtgcgt gacatcgatc cgggcggtga cgcggggaaa caggttgata accggggtac
     9901 agaacaggct gaactgtgcc gcatttgtct ggtggatcag ccagtccggc ggcaggcggt
     9961 tcagcaggat aacaatctcc gccacgccac cgtcaatttt ctgtaatccg gcagacaggc
    10021 ctgtcggggt gaaaaaatag aagcgttccg gacaggcaaa atattcatgc agcaggttat
    10081 gaccgtgaaa cacgttccac gccagcggca gcagcccctg atccggctcc aggccttcgt
    10141 acataacggg ctgttgcaga ttcacatcca gcgcgccgtc aaaatgcccc ggaatgccag
    10201 ccaggggcgc gatggcgctg gtatgtagaa gttctaacag gtgtgaggcc gtacgctcct
    10261 cgccgcagag ataaaacggc agcctgtcca gtccggcaag ctgactgaac gttagttcac
    10321 caaaggtgcg cagggtaata cgcagcgccc cggcgacgtg aatgttggcc ggcaggtagc
    10381 ggtgcagggc aggcatatcc ggtggcgcgg cggtcagtcg cgcctcttcg atggcgagcg
    10441 gccacagcgt tacatcctgg ctgctgcgaa actggcaggc agtgctttcg ccttccggaa
    10501 tggcggagaa aaaggctgtg tcgcgcggga cggtaaatcc tttcgccaga tcgccttctt
    10561 ccgtatcggg atgcaactgc gccacgctca ttgagggcgt gggtgtgacg taattggggc
    10621 tgaccacttc cagcaggcgc tgggtgaaac gggggaattc agcgtcaatt tttagctgcg
    10681 tgcgggcggt caggaagctg aaggcctcga ccattcgctc tacatacgga tcggcaatat
    10741 ccgttccctg catgcccagc cgggcggcga ctttgggatg acgggcggca aattcggctc
    10801 ccgtttcccg cagatagctc agttcgcggt tgtagtattc cagtagccgt ggatccatcg
    10861 gtctctctct gaattaaaaa aacgtaatgc gactctgctc catatccagc gcgctacgta
    10921 cccggaatgc agtcggatac ggctgcgtga ggatctcgcc gcgaatttca aactgtaatg
    10981 tgttatggcc actctggcgg cgctcatcct gaagtgggat gatttgcaga gtggacgctt
    11041 tcagccgggg ttcaaaccgg gtgatggttc gcaaaatggc cctgcggata tcgtcccact
    11101 tgtgctcata cagaaagctg cccgccagtg gcggcaggcc gtagttaagt acggacgctg
    11161 ccgcgtacgg ataacgtgca gcatcaatat catcttcatg gctgacggtg ttgagcagaa
    11221 atgacaaatc ccggcggatg atctccttaa tctgaaccgg actgacgctg ataccgtggt
    11281 cgcgtttctg gtttggggcg tcgtcacaca ggcggtcaaa cagcgtgggc agcaggtgat
    11341 tgcctggacg ctggcgggaa gcgctcacgc gtcgcttcct tcagcggtgt taaaggtaca
    11401 ggtggacaaa tccagcaggc tgatatcgcc gtgaccggtg agccacaccc tctgacccag
    11461 cgcctgtacc agtgtctctc ccggaccgtc ctgccagacc gtttcccggc acagacgcag
    11521 ggagtcggca gccttttcag agccgctgta gcggggaaac agctaggcac tgtgtgtatc
    11581 gccattgttc agggtgacgt tgacgggttt ccagatcaaa tcgatcagat gcgcaggccg
    11641 cggcgatttc agcgaacaga tttgtgaaaa cggcagccag atatatgctc cgcccgcgat
    11701 cagttcgagt accggaccgg tacgggagtc gctgtcgcta atccagtcaa atgcgccgcc
    11761 attccactgc ccggaagtgt ctgttatggc ctcaagcgcg cggctgcggt gagcatcagc
    11821 ttcctgcgct tcaccacggg cgttacaggc gagggcggtc agcagatcct ccatccaggc
    11881 gggcggcggg agaataacac cgggccgctg ttcgccctga aagcaggcgt ggcggtatat
    11941 ctcacagcgg atcagctcgc caaatacctg cgcttcgcga gtataatttg cgtccatccg
    12001 ggcgcatagc tggatctgtt gcagtgcccg cgaccagtcg cctgccacgc acaataactg
    12061 aaacaggctg tggcggcggg aggcctgcgc tgggtgcatc ctgacctgtt gctctgccat
    12121 ctgaatgccc tccaccacgg aataatcccg cagcagcgcc gacaaggtaa tcggtagctc
    12181 gttattgttt gtcatgatat atctctccat gttgtgccgg gtttatccgg taatggctgt
    12241 caatgccaat gatgcgatgc tctttccgtg tcagatctgg cagtacggtg ttatgcgctg
    12301 tttccggggc gtattccggg gaaagtaaat gcaggatatc agggggagtc ttttcgacag
    12361 gccagatagt ttcatcttcg ccagtggcat ccagtacatt gagaatagcg tcaatgctta
    12421 acccaccagt gaccatatcc tgcaatgtct cggcttcggt gaccaggtca cgcaatgaat
    12481 ccagatcctg cgaggagggc tcttcgttaa aaggaaaagg atcagcatgg cgattaagat
    12541 cttgttcggg agataacaac gcctcgcggt attcctgata aagtcgggtc agggcatttc
    12601 ctttttccgc accttctccg gtggaggagg ctccatgaac aggaatgata tcgaatggat
    12661 tttgtgatgt cagatgctgc tgcctgaacc aatccagatc cagatattcg gttattgctt
    12721 ccgacgtgat ggcagtctgc gatatcgttt ttactgtgct caggttcgga gatgatggat
    12781 tgccatgtac aaggcactat gaagataacc cccattctat caggtcgcct tcattcagac
    12841 gcatacggtg atgaggttcc acaatctggt cgtttaccgt acagcaatat tcggtagact
    12901 gattgataat ccaccatgct tcttcgtggc gggtcagagt gagggcaatc tttacatcat
    12961 cattcacctt aaaaacagga atccagggag aggtaacggt gaagataatt tgtgttcctt
    13021 cagtcgcgta aattgtgtta tcggcgatcc cgtgcgcctg tattttttgg agtccccacg
    13081 taatat
//
LOCUS       DAJTUI010000098        12980 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTUI010000098 DAJTUI010000000
VERSION     DAJTUI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.098"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(5..1066)
                     /locus_tag="OO464_003202"
     CDS             complement(5..1066)
                     /locus_tag="OO464_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6228244.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1279..2742
                     /gene="bepA"
                     /locus_tag="OO464_003203"
     CDS             1279..2742
                     /gene="bepA"
                     /locus_tag="OO464_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6228245.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            2763..3122
                     /gene="arsC"
                     /locus_tag="OO464_003204"
     CDS             2763..3122
                     /gene="arsC"
                     /locus_tag="OO464_003204"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6228246.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3255..4001)
                     /gene="hda"
                     /locus_tag="OO464_003205"
     CDS             complement(3255..4001)
                     /gene="hda"
                     /locus_tag="OO464_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6228247.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4051..5340)
                     /gene="uraA"
                     /locus_tag="OO464_003206"
     CDS             complement(4051..5340)
                     /gene="uraA"
                     /locus_tag="OO464_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6228248.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5426..6052)
                     /gene="upp"
                     /locus_tag="OO464_003207"
     CDS             complement(5426..6052)
                     /gene="upp"
                     /locus_tag="OO464_003207"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6228249.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6376..7413
                     /gene="purM"
                     /locus_tag="OO464_003208"
     CDS             6376..7413
                     /gene="purM"
                     /locus_tag="OO464_003208"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6228250.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7413..8051
                     /gene="purN"
                     /locus_tag="OO464_003209"
     CDS             7413..8051
                     /gene="purN"
                     /locus_tag="OO464_003209"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6228251.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8223..10289
                     /gene="ppk1"
                     /locus_tag="OO464_003210"
     CDS             8223..10289
                     /gene="ppk1"
                     /locus_tag="OO464_003210"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6228252.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10294..11835
                     /gene="ppx"
                     /locus_tag="OO464_003211"
     CDS             10294..11835
                     /gene="ppx"
                     /locus_tag="OO464_003211"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6228253.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(11874..>12980)
                     /gene="pdeF"
                     /locus_tag="OO464_003212"
     CDS             complement(11874..>12980)
                     /gene="pdeF"
                     /locus_tag="OO464_003212"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6228254.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         3356 a         3368 c         3271 g         2985 t
ORIGIN      
        1 aagattattc ttgcgcgatt tgcccatcgg gccaggcgtg gatcacggct ttgatcagcg
       61 tcgccaatgg aatggcgaag aatacgcccc agaatcccca caaaccaccg aagatcacca
      121 ccgataaaat aatcaccagc ggatgcaggt taaccgcttc ggagaacaac accggtacta
      181 gcaggttgcc gtccagcgcc tgaataatca gatacaccgc gaagcagctc cagaattcag
      241 tacctgcgcc aaactggaat agcgccacgc caaccaccgg aatggtcacc acaaatgcgc
      301 caatgtacgg aataagaacc gagaagccga ccagcactgc cagcagcagc gaatagttca
      361 gcccgaagag caagaacccc agccaggtgg cgatccccac cacgatcatc tccagcactt
      421 tgccgcggat atagttggtg atttgttgat tcatctcctt ccacacctgt cctgccagcc
      481 cacggttacg cggcagcacc cggcgaacgg cgttcagcat ctgctctttg tctttcagca
      541 ggaagaagac catcaatggc accagcacca gatagacggc tatggtcagc aaaccgacca
      601 gcgaggcgag ggaaattttc accaccgaat cgcccatggt cagcatccga ctgcgcatat
      661 tttcggccat tgcatcaata atgcccgcgt ccattaacac cggatagcgg cgcggcaacg
      721 tggcggcaaa gtcagaaagc ttattgagca tccccggcat atcgcggatt aagtagatgc
      781 cctgttgcca ggcgataggc agtaccacga acgccatcag tagcaatata ccgacaaaaa
      841 ccaccaatac aatcgacgtc gcccagcggc gggagcagcc aatagattgc aggcgcacgg
      901 ttggccattc cagcaaataa gccagcacaa tagccaccag caacggagca agcaggccac
      961 taaagaaaaa gataatgcca aatccggcaa ctaaaataac cagcaaggca atcgcttccg
     1021 ggtcgctaaa acggcggcga taccattgca tcaacatttc gagcataaag accttccctg
     1081 aacctcaaga gcgggattgc gatccgcaat tgtatcgaaa tgtcacaaaa aagacttcgc
     1141 tttttatgac ggattcagga aactgaaaag tcatttgagt gggctaatct tcgccgttac
     1201 actcaaaggc ggcgaggtgg gaacgatatt tcacagtatc ggtcaaatga ctatcttcag
     1261 aaatacagga tagaggttat gttcaggcag ttgaaaaaaa acctggttgc aaccctcatt
     1321 gctgctatga ccattggtca ggtagccccg gcgtttgccg acagcgcaga caccttgccg
     1381 gatatgggaa cctccgcagg aagcacgctt tccattggtc aggaaatgca gatgggcgac
     1441 tattatgtcc gccagctacg cggcagcgcg ccgttaatta atgacccgct gttaacgcaa
     1501 tatattaatt cgctggggat gcgtctggtt tcgcatgcca attcggttaa gacaccgttt
     1561 catttttttc tgatcaacaa cgacgaaatt aacgcctttg ctttctttgg cggcaacgtg
     1621 gtgctgcact ctgccctgtt ccgttattcc gataacgaaa gtcaactggc ttcagttatg
     1681 gcgcacgaaa tctcccacgt cacccaacgt cacctggcgc gagcgatgga agatcaacaa
     1741 cgcagcgcgc cgctgacctg ggtcggcgcg ttaggttcta ttttactggc gatggccagt
     1801 ccgcaggcgg ggatggcggc gctgaccggt acactggcgg gaacgcgcca ggggatgatc
     1861 agtttcaccc agcaaaatga acaggaagcg gaccgcattg gtattcaggt gctgcaacgc
     1921 tcgggattcg atccacaggc gatgccgacc ttcctggaaa aattgctcga tcaggcgcgt
     1981 tactcctcgc gcccgccgga aattttattg actcacccgt tgccggaaag tcgtctggca
     2041 gatgcccgca accgtgctaa tcagatgcgc ccgatggtgg tgcagtcgtc ggaagatttc
     2101 tatctggcga aagcgcgcac actggggatg tataattccg gacgtaacca gctcaccagt
     2161 gatttgctgg atgaatgggc gaaaggaaac gttcgtcagc aacgagcggc gcaatatggt
     2221 cgtgctttac aggcgatgga agccaataaa tacgacgagg cgcgtaaaac gctgcaaccg
     2281 ttactggcgg cagaacctgg caacgcatgg tatctcgatc tggctaccga tatcgatctt
     2341 gggcaaaaca aagccaatga ggcaatcaat cgcctgaaaa atgcccgcga tttacgcacc
     2401 aatccggtgt tgcagctcaa cctggcgaac gcttatctgc aaggcggtca accacaagaa
     2461 gcggccaata ttctgaatcg ctacaccttt aataataaag atgacagcaa cggctgggat
     2521 ttgctggcac aggcggaagc cgcgctaaat aaccgcgatc aggagctggc tgcgcgagca
     2581 gaaggttatg cgctcgccgg acgactcgat caggccattt cgctgttgag tagcgccagt
     2641 tcgcaggtga aattaggcag cctgcaacaa gcgcgttacg atgcgcgcat cgaccagttg
     2701 cgccagctgc agaaacgctt taagccttat accaagatgt aattcgcatt aaaggaagaa
     2761 caatgaccaa acaggtaaaa atctaccata acccacgctg ttcaaagagc cgggaaacgc
     2821 tgaacctgct gaaagaaaac ggcgtggagc cagaagtggt gctctacctt gagacacccg
     2881 ccgatgcggc aacgctgcgc gatttgctga aaatgctggg gatgaatagc gcccgtgaat
     2941 tgatgcgcca gaaagaggat ctttataaag aactaaacct ggctgacagt tcgcttagcg
     3001 aagaggcgct gattcaggca atggtggata atccgaagct gatggagcgc ccgattatgg
     3061 tcgcgaatgg caaagcgcgg attggtcgtc caccggagca ggtactggaa atcgtgggtt
     3121 aagatgtgct ccatcgctag ttgaagcaca ttgccggatg cgacgcttgc cacgtcttat
     3181 ccggcctacg aatactatgg attaacttcg cgtaggtcag gcgttcgcgc cgcatccgac
     3241 aataaacacc ttatctacaa cttcagaatt tctttcacaa acggaatggt cagcttacgt
     3301 tgcgcggtaa tcgacgcacg atccaactga tccaacgtca taaatagcgt gcgcatttct
     3361 ctgtcgagcc gcttcagcaa gaaacgcccc acatcttccg gcagttcaaa accacgcaaa
     3421 cgcgcgcgta actgtagcgc ctgcaactta tcttcatcag aaagtggctg caatttgtag
     3481 atctgccccc agtcgagacg cgacgcgaga tccggtaatc ccagattcaa ctgccgcggt
     3541 ggacgatcgc cggtgatcaa caaccgtgtt ttgcccgatt ccagaattcg attgtagaga
     3601 tcgaaaatcg ccatctccca caactcatcg cctgcaatac actcaatgtt gtcgatacag
     3661 accagcgaca aatgctccat accgtcgagc acttccggaa caaaccaggt gcgtttatcc
     3721 agcgggacat agcccaccgc atcgccacgc tgcgacaatt ccgcgcaagc cgcgtgcagc
     3781 agatggctgc gccccgcgcc ttcgcgtgcc cagagataga tgtaaccgct atgttcctga
     3841 cgcagcacgt tttgcagcgc ggccagtaaa gaggagttat cccccggcca gaaacttgca
     3901 aaggtttcgt cgtcaggaag ataaagtggc aaagagagct gtgccggtgt gttcagagat
     3961 acctcaacca ggatttcaca aaatcgcgag aagtttacca cagaatccca tgatgtttga
     4021 accgggcagc aacactgccc ggtcggtaca ttatttgtct gttatatccg cgtcttctgc
     4081 gtccagcacc acttcttcag ggcgcagcac actgatcagt ttgaaaatca aacttaagcc
     4141 gataccgacg atggtcgcca gcgccatacc ttttaactcc gccgcgccga tgtttacctt
     4201 cgcgccactg acgccgatga tcaaaatcac ggaagtcagg atcaggttct gtgctttgtt
     4261 gtaatccact ttcgattcga tcaaaacacg aataccggaa gcaccaatga caccataaag
     4321 cagcagcgaa acgccgccca tcaccggcaa tgggatcatc tggatagcgg cagccagttt
     4381 accgacgcag gaaagcagga tagcgaaaat cgccgccccg ccgataaccc aggtactgta
     4441 aacacgggtg atcgccatca cgccaatgtt ttctccgtaa gtggtatttg gcgtagagcc
     4501 aaagaagccg gagatcacgg tcgacaggcc gttagcaaac atcgaacggt gcagacctgg
     4561 atcgcgcagc agatcttttt tgacgatatt agccgttact accaggtgcc ctacgtgttc
     4621 ggcaataacc actaacgccg ctggcagaat agtcagaatg gcaaaccact cgaagcgcgg
     4681 cgtatagagg gttggcagtg caaaccagtg agcattaata atcggcgtgg tatcgacaat
     4741 tcccattgcg aaagagagcg cgtaccccac cagcacgcca attaaaatcg ggataattgc
     4801 caggaaacca cgaaacagca cggaacctaa aaccgtgacc gccagggtgg taatagagat
     4861 gatgatggtt ttggagtttg gcgtttgccc ttcagccggg agtaaacccg ccataccggc
     4921 agctacgccc gccagctcca gaccgatgac ggcaacgatt gcgcccattg ccgcaggtgg
     4981 aaacagcacg tccaaccagc cggtccccgc tttcttcacg ataaaagaaa ccaggcagaa
     5041 cagcacgccg cacataataa agccgcccag cgcgacttca taccctaacg gcaacagtaa
     5101 caataccggt gaaataaagg caaagctgga accaagataa gccggaattt tccctttaca
     5161 gatgaagaga tacagcagcg ttccaatacc gttaaataac agtacagtcg ccgggttaat
     5221 atgaaataag acgggcacca ggacggttgc accaaacatg gcgaacaaat gttgcaaact
     5281 aagcgggatt gtctgtaaaa gtggcggtct ttcactcacc ccgatagcac ggcgcgtcat
     5341 agtattatcc tctgtattat gtgttatagg cgctttactc aaaaaaaagc cgactcttaa
     5401 agtcggcttt aattattttt attctttatt tcgtaccaaa gattttgtca ccggcatcgc
     5461 cgaggcccgg aataatgtat ccgtgctcgt tcagtccctg gtcaatcgat gcagtataca
     5521 gttcgacgtc cgggtgcgct ttttccagcg cagcgatacc ttctggcgca gcgaccagca
     5581 ccagaacttt gatgctgctg cagcccgctt ttttcagcag gtcgatggtc gcgataacgg
     5641 aaccaccggt tgccagcatt gggtcaacga tcagcgccat acgctcatcg atgttagaaa
     5701 ccagtttctg gaagtacggt accggctcca gcgtttcttc attacggtac ataccgacaa
     5761 cgctgatgcg cgcgctcgga acgttttcca gcacaccgtc catcatacca agacccgcac
     5821 gcagaattgg cacaacggta attttcttac ctttgatctg gtcgatttct accgggccgt
     5881 tccagccttc gatagttact ttttccgttt cgaggtcggc ggtcgcttcg taagtcagca
     5941 ggctacccac ttcggaagcg agttcgcgaa agcgcttggt gctgatatct tgctcacgca
     6001 tcagtcccag cttgtgtttg acgagtgggt gtttgacttc cacgatcttc atactctttc
     6061 tcctttgagg ggcagccaca aaaaaatcga cggattatac ctcctttctt cagggcggca
     6121 atattatttt cgttgacttt agtcaaaatg ataacagttt gagataaagt tattttatat
     6181 tcaaatggtt atgaaagaag attattccat ccgaaaacta acctttgccc tggcacaagt
     6241 cttctttcgc cgcgcgcccg gggaaaagac gtgcaaaaag gttgtgtaaa acagtctcgc
     6301 aaacgtttgc tttccctgtt agaattgcgc cgaattttat ttttctaccg caagtaacgc
     6361 gtggggaccc aagcagtgac cgataaaacc tctcttagct acaaagatgc cggtgttgat
     6421 attgacgcgg gtaatgctct ggttggaaga atcaaaggcg tagtgaagaa aacgcgtcgt
     6481 ccggaagtga tgggcggtct gggcggcttc ggtgcgctgt gtgcattgcc gcaaaaatat
     6541 cgtgaacccg tgctggtttc cggcactgac ggcgtaggta ccaagctgcg tctggcaatg
     6601 gacttaaaac gtcacgacac cattggtatt gatctggtcg ccatgtgcgt taatgacctg
     6661 gtggtgcaag gtgcggaacc gctgtttttc ctcgactatt acgcaaccgg aaaactggat
     6721 gttgataccg cttcagcggt gatcagcggc atcgcggaag gttgtctgca atcgggctgt
     6781 tctctggtgg gtggcgaaac ggcagaaatg ccggggatgt atcacggtga agattacgat
     6841 gtcgcgggtt tctgcgtggg cgtggtagaa aaatcagaaa tcatcgacgg ctctaaagtc
     6901 agcgacggcg atgtgctgat tgcactcggt tccagcggtc cgcactcgaa cggctattcg
     6961 ctggtgcgca aaattcttga agtcagcggt tgtgatccgc aaaccaccga acttgatggt
     7021 aagccattag ccgatcatct gctggcaccg acccgcattt acgtgaagtc agtgctggag
     7081 ttgattgaaa aggtcgatgt gcatgccatt gcgcacctga ccggcggcgg cttctgggaa
     7141 aacattccgc gcgtattgcc agataatacc caggcagtga ttgatgaatc ttcctggcag
     7201 tggccggaag tgttcaactg gctgcaaacg gcaggtaacg ttgagcgcca tgaaatgtat
     7261 cgcaccttca actgcggcgt cgggatgatt atcgccctgc ctgctccgga agtggacaaa
     7321 gccctcgccc tgctaaatgc caacggtgaa aacgcgtgga aaatcggtat catcaaagcc
     7381 tctgattccg aacaacgcgt ggttatcgaa taatgaatat tgtggtgctt atttccggca
     7441 acggaagtaa tttacaggca attattgacg cctgtaaaac caacaaaatt aaaggcaccg
     7501 tacgggcagt tttcagcaat aaggccgacg cgttcggcct tgaacgcgcc cgccaggcgg
     7561 gtattgcaac gcatacgctc atcgccagcg cgtttgacag tcgtgaagcc tatgaccggg
     7621 agttgattca tgaaatcgac atgtacgcac ccgatgtggt cgtgctggct ggttttatgc
     7681 gcattctcag cccggcgttt gtctcccact atgccgggcg tttgctgaac attcaccctt
     7741 ctctgctgcc gaaatatccc ggattacaca cccatcgtca ggcgctggaa aatggcgatg
     7801 aagagcacgg tacatcggtg catttcgtca ccgatgaact ggacggtggc ccggttattt
     7861 tacaggcgaa agtcccggta tttgctggtg atacggaaga tgacatcacc gcccgcgtgc
     7921 aaacccagga acacgctatt tatccactgg tgattagctg gtttgccgat ggtcgtctga
     7981 aaatgcacga aaacgccgcg tggctggatg gtcaacgtct gccgccgcag ggctacgctg
     8041 ccgacgagta atgcccccgt agttaaagcg ccagctctgc cgctggcgtt tttcaattca
     8101 cctgtaaatc gcaagctcca gcagtttttt tccccctttt ctggcatagt tggacatctg
     8161 ccaatattgc tcgccataat atccaggcag tgtcccgtga ataaaacgga gtaaaagtgg
     8221 taatgggtca ggaaaagcta tacatcgaaa aagagctcag ttggttatcg ttcaatgaac
     8281 gcgtgcttca ggaagcggcg gacaaatcta acccgctgat tgaaaggatg cgtttcctgg
     8341 ggatctattc caataacctt gatgagttct ataaagtccg cttcgctgaa ctgaagcgac
     8401 gcatcattat tagcgaagaa caaggctcca acgctcattc ccgccattta ctgggcaaaa
     8461 ttcagtcccg ggtgctgaaa gccgatcagg aattcgacgg cctctacaac gagctattgc
     8521 tggagatggc gcgcaaccag atcttcctga ttaatgaacg ccagctctcc gtcaatcaac
     8581 aaaactggct gcgtcattat tttaagcagt atctgcgtca gcacattacg ccgattttaa
     8641 tcaatcctga cactgactta gtgcagttcc tgaaagatga ttacacctat ctggcggtgg
     8701 aaattatccg tagcgatagc atccgttacg cgctgctgga gatcccatca gataaagtgc
     8761 cgcgctttgt gaatttaccg ccagaagcgc cgcgtcgacg caagccgatg attcttctgg
     8821 ataacattct gcgttactgc cttgatgata ttttcaaagg cttctttgat tatgacgcgc
     8881 tgaatgccta ttcaatgaag atgacccgcg atgccgaata cgatttagtg catgagatgg
     8941 aagccagcct gatggagttg atgtcttcca gtctcaagca gcgtttaact gctgagccgg
     9001 tgcgttttgt ttatcagcgc gatatgccca atgcgctggt tgaagtgtta cgcgaaaaac
     9061 tgactatttc ccgctacgac tccatcgtcc ccggcggtcg ttatcataat tttaaagact
     9121 ttattaattt ccccaatgtc ggcaaagcca atctggtgaa caaaccactg ccgcgtttac
     9181 gccatatttg gtttgataaa gcccagttcc gcaatggttt tgatgccatt cgcgaacgcg
     9241 atgtgttgct ctattatcct tatcacacct ttgagcatgt gctggaactg ctgcgtcagg
     9301 cttcgttcga cccaagcgta ctggcgatta aaattaacat ttaccgcgtg gcgaaagatt
     9361 cacgcatcat cgactcgatg atccacgccg cacataacgg taagaaagtc actgtggtgg
     9421 ttgagttaca ggcgcgtttc gacgaagaag ccaacattca ctgggcgaag cgcctgaccg
     9481 aagcaggcgt gcacgttatc ttctctgcgc cggggctgaa aattcacgcc aaactgttcc
     9541 tgatttcacg taaagaaaac ggtgaagtgg tccgttacgc acacatcggg accgggaact
     9601 ttaacgaaaa aaccgcgcgt ctttatactg actattcgtt gctgaccgca gatgcgcgta
     9661 tcaccaacga agtacggcgg gtatttaact ttattgaaaa cccataccgc ccggtgacat
     9721 ttgattattt aatggtgtca ccgcaaaact ctcgccgtct gttatatgaa atggtagacc
     9781 gcgaaatcgc caacgcgcag caagggctgc ccagtggtat caccctgaag ctaaataacc
     9841 ttgtcgataa aggcctggtt gatcgtctgt atgcggcctc cagctccggc gtaccggtta
     9901 atctgctggt tcgcggaatg tgttcgctga tccccaatct ggaaggcatt agcgacaaca
     9961 ttcgtgccat cagtattgtt gaccgttacc ttgaacatga ccgggtttat atttttgaaa
    10021 atggcggcga taaaaaggtc tacctttctt ccgccgactg gatgacgcgc aatattgatt
    10081 atcgtattga agtggcgaca ccgctgctcg atccgcgcct gaagcagcgg gtgctggaca
    10141 tcatcgacat attgttcagc gatacggtca aagcacgtta tatcgataaa gaactcagta
    10201 atcgctacgt tccccgcggc aatcgccgca aagtacgggc gcagttggcg atttacgact
    10261 acatcaaatc actcgaacaa cctgaataac cctatgccaa tacacgataa atcccctcgt
    10321 ccgcaggagt ttgctgcggt cgatcttggt tcaaacagtt ttcacatggt catagcccgt
    10381 gtggtagatg gtgccatgca gattattggc cgcctgaaac agcgggtgca tctggcggac
    10441 ggcctggggc cagataatat gttgagtgaa gaggcaatga cgcgcggttt gaactgtctg
    10501 tcgctgtttg ccgaacggct acaagggttt tctcctgcca gcgtctgtat agtcggcacc
    10561 catacgctgc gtcaggcgct gaacgccact gactttctga aacgcgcgga aaaggtcatt
    10621 ccctacccga ttgaaattat ttccggtaat gaagaagccc gtctgatttt tatgggcgtg
    10681 gaacataccc aaccggaaaa aggtcgcaaa ctggttattg atattggcgg cggatctacg
    10741 gaactggtga ttggtgaaaa tttcgaacct attctcgttg aaagccgccg gatgggttgt
    10801 gtcagctttg cccagcttta ttttcctggc ggggtcatca ataaagagaa ttttcagcgc
    10861 gctcgcatgg cggcagcaca aaaactggaa actttaacct ggcaattccg tattcagggc
    10921 tggaacgttg caatgggcgc ttccggtacc ataaaagccg cccatgaagt gttaatggaa
    10981 atgggcgaga aagacgggat aattaccccg gaacgtctgg aaaaactggt aaaagaaatt
    11041 ttacgtcacc gtaatttcgc atcgctgagt ttaccgggtc tttccgaaga gcggaaaaca
    11101 gtcttcgttc ctggactggc gattttatgt ggtgtgtttg atgctttagc catccgtgaa
    11161 ctgcgccttt ctgacggggc gttacgcgaa ggcgtactgt atgaaatgga aggacgtttc
    11221 cgtcatcagg atgtgcgtag tcgcaccgcc agcagcctcg ccaaccagta tcacatagac
    11281 agcgaacagg cccgacgagt gctggatacc actatgcaaa tgtacgaaca gtggcgggaa
    11341 cagcaaccga agctggcgca tccgcaactg gaggcgctac tgcgatgggc cgccatgctg
    11401 catgaggtcg ggttgaatat caaccacagc ggtttgcatc gccactccgc ttatattctg
    11461 caaaacagtg acttgccggg ttttaatcag gaacagcagc tgatgatggc gacactggtg
    11521 cgctatcacc gtaaagcgat taagctcgac gatctgccgc gctttacctt gtttaagaag
    11581 aaacagttcc tgccactgat acagctattg cgccttggcg tattactaaa caatcaacgt
    11641 caggcaacca ccacaccgcc aacattgaca ctgattactg atgacagtca ctggacactg
    11701 cgtttcccgc atgactggtt tagtcagaat gcgctggtac tgcttgatct ggaaaaggag
    11761 caagaatact gggaaggcgt ggctggctgg cggttgaaaa ttgaagaaga aagtacccca
    11821 gaaatcgccg cttaatgctt tgcgggccga cgagaaatgt cggcccgcat tattcaggca
    11881 ctttcgcgaa tgggttcgat ttcattcagc gtatcaatta acggttgcgg cttaccaata
    11941 agataaccct gcatataatc gatccccaaa gagagcaccg cctcgcggat ctcttcgttt
    12001 tcaacgtact ctgccactac ccgcattttc ttcattcgcg ccagatggca aatcgacgcc
    12061 acaatctgat aatccagact atttgacaca atattgcgga taaagctgcc gtcaatttta
    12121 agcagatcgg cattcacatt tttaagccgt gcatagctgg cgtagccggt gccgaaatca
    12181 tcaatcgcaa tctggcagcc taattcctga agatgctgca aggtgatttg cgcctgctta
    12241 acattggtca gagcattact ttcggtgact tcaaaaataa gttgccacgc ttcaatctga
    12301 tatttagcca gcaactgact gacttcaaca ggaaaacgag cctgacatac cgaggttgga
    12361 gacagattaa tagcaaaacg gtgagcgggc atcttcgctc tgttttcagc cataaattgc
    12421 agcgtatgct caatgaccca catgtcgata ctcgacgata aaccaaattc gtgcgcgacc
    12481 ggcaagaagc taccggggcc gatcagttca tcattctcac ctttcatgcg cagaagaatt
    12541 tcatggtaaa catcaccacg cataccggta atcggctggg ccatcaggaa aaaatggttg
    12601 tgttccagcg cctgctgtag tcgattcatc atcgcgactt tatctttcaa ttcgcgttgc
    12661 aaatacattg ccccgcaacg ctgcatattt tccggggcgt tggtcacgat ggaaagttcg
    12721 gcgaccgtat ttagctctcc cagcagcagg tagatatgat tcactggcga gcgcacatag
    12781 cagtaactga cgccaatctg cggttgcatc ggcatcccat cccaaaagaa acgaaattgc
    12841 ttgagatgac tatccagtgc ggtaatgcgc tcctggtgcg attctgtatt cagtcgcagc
    12901 gcgagatcgt tacccgaaag ctgataaaca tcttcacccg gttccagcaa gggtgacagc
    12961 cagtgagaaa gtttttgctt
//
LOCUS       DAJTUI010000099        12903 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTUI010000099 DAJTUI010000000
VERSION     DAJTUI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.099"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO464_003213"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO464_003213"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR6228255.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="OO464_003214"
     CDS             complement(1806..3224)
                     /locus_tag="OO464_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6228256.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="OO464_003215"
     CDS             complement(3378..3722)
                     /locus_tag="OO464_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR6228257.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO464_003216"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO464_003216"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR6228258.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="OO464_003217"
     CDS             complement(4712..5647)
                     /locus_tag="OO464_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR6228259.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="OO464_003218"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="OO464_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR6228260.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="OO464_003219"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="OO464_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR6228261.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO464_003220"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO464_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR6228262.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9080..9514
                     /gene="marR"
                     /locus_tag="OO464_003221"
     CDS             9080..9514
                     /gene="marR"
                     /locus_tag="OO464_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR6228263.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9534..9917
                     /gene="marA"
                     /locus_tag="OO464_003222"
     CDS             9534..9917
                     /gene="marA"
                     /locus_tag="OO464_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR6228264.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9949..10167
                     /gene="marB"
                     /locus_tag="OO464_003223"
     CDS             9949..10167
                     /gene="marB"
                     /locus_tag="OO464_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR6228265.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10198..11097)
                     /gene="eamA"
                     /locus_tag="OO464_003224"
     CDS             complement(10198..11097)
                     /gene="eamA"
                     /locus_tag="OO464_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR6228266.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11361..12479
                     /gene="ydeE"
                     /locus_tag="OO464_003225"
     CDS             11361..12479
                     /gene="ydeE"
                     /locus_tag="OO464_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR6228267.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12606..12701
                     /gene="mgtS"
                     /locus_tag="OO464_003226"
     CDS             12606..12701
                     /gene="mgtS"
                     /locus_tag="OO464_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR6228268.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3399 a         3298 c         2950 g         3256 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcatttga cttatacttg cctgggcaat attaccccct acaactaatt
     9061 acttgccaag gcaactaatg tgaaaagtac cagcgatctg ttcaatgaaa ttattccatt
     9121 gggtcgctta atccatatgg ttaatcagaa gaaagatcgt ctgcttaacg agtatctgtc
     9181 tccgctggat attaccgcgg cacagtttaa ggtgctctgc tctatccgct gcgcggcgtg
     9241 tattactccg gttgaactga aaaaagtgtt gtcggtcgac ctgggagcac tgacccgtat
     9301 gctggatcgc ctggtctgta aaggctgggt tgaaaggttg ccgaacccga atgataagcg
     9361 cggcgtactg gtaaaactta ccaccagcgg cgcggcaata tgcgaacaat gccatcaatt
     9421 agttggccag gacctgcatc aagaattaac aaaaaacctg acggcggacg aagttgcaac
     9481 acttgagcat ttgcttaaga aagtcctgcc gtaaacaaaa aagaggtatg acgatgtcca
     9541 gacgcaatac tgacgctatt accattcata gcattttgga ctggatcgag gacaacctgg
     9601 aatcgccact gtcactggag aaagtgtcag agcgttcggg ttactccaaa tggcacctgc
     9661 aacggatgtt taaaaaagaa accggtcatt cattaggcca atacatccgc agccgtaaga
     9721 tgacggaaat cgcgcaaaag ctgaaggaaa gtaacgagcc gatactctat ctggcagaac
     9781 gatatggctt cgagtcgcaa caaactctga cccgaacctt caaaaattac tttgatgttc
     9841 cgccgcataa ataccggatg accaatatgc agggcgaatc gcgcttttta catccattaa
     9901 atcattacaa cagctagttg ataacgtgac aacgtcactg aggcaatcat gaaaccactt
     9961 tcatccgcaa tagcagctgc gcttattctc ttttccgcgc agagcgttgc ggaacaaacc
    10021 acgcagccag ttgttacttc ttgtgccaat gtcgtggttg ttcccccatc gcaggaacaa
    10081 ccaccgtttg atttaaatca catgggtact ggcagtgata agtcggatgc gctcggcgtg
    10141 ccctattata atcaacacgc tatgtagttt gttctggccc cgacatctcg gggcttatta
    10201 acttcccacc tttaccgctt tacgccaccg caagccaaat acattgatat acagcccggt
    10261 cataatgagc accgcaccta aaaattgcag acccgttaag cgttcatcca acaatagtgc
    10321 cgcacttgcc agtccgacta cgggcaccag taacgataac ggtgcaaccc gccaggtttc
    10381 atagcgtccc agtaacgtcc cccagatccc ataaccaaca attgtcgcca caaacgccag
    10441 atacatcagc gacaagatgg tggtcatatc aatagtaacc agactgtgaa tcatggatgc
    10501 ggaaccatcg agaatcagcg agacaacaaa gaagggaatg attgggatta aagcgctcca
    10561 gattaccagc gacatcaccg ccggacgcgt tgagtgcgac atgatctttt tattgaagat
    10621 gttgccacac gcccaactaa atgctgcagc cagggtcaac ataaagccga gcatcgccac
    10681 atgctgaccg ttcagactat cttcgattaa caccagtacg ccaaaaatcg ctaaggcgat
    10741 ccccgccaat tgtttgccat gcagtcgctc cccgaaagta aacgcgccaa gcacgatagt
    10801 aaaaaacgcc tgtgcctgta acaccagcga agccagtcca gcaggcatac caaagttaat
    10861 ggcgcagaac agaaaggcaa actgcgcaaa actgatggtt aatccatacc ccaagagcaa
    10921 attcagtggt actttcggtc gtgcaacaaa aaagatggcc ggaaaagcga ccagcataaa
    10981 gcgcaaaccg gccagcatca gcggtggcat gttatgaagc cccactttga tgaccacaaa
    11041 atttagcccc catacgacca ctaccagtag cgccaacacc ccatcttttc gcgacattct
    11101 accgcctctg aatttcatct tttgtaagca atcaacttag ctgaatttac ttttctttaa
    11161 cagttgattc gttagtcgcc ggttacgacg gcattaatgc gcaaataagt cgctatactt
    11221 cggatttttg ccatgcgtcc gatggttaat aacaacgacg aggcaagaag ggcgctggta
    11281 gagcgtcgta gggataagtt catttgtctg tccggcagtg cgtttcgtta tgttttattt
    11341 tagagatgta aagaaatagc atggctacgc tgccatttat gaccatttac ttgagtcgcc
    11401 agtacagcct gagtgtcgat ctaatcggtt atgcgatgac aattgcgctc actattggcg
    11461 tcgtttttag cctcggtttt ggtatcctgg cggataagtt cgacaagaaa cgctatatgt
    11521 tactggcaat taccgccttc gccagcggtt ttattgccat tcctttagtg aataacgtga
    11581 cgctggttgt gctctttttt gccctcatta actgcgccta ttctgttttt gctaccgtgc
    11641 tgaaagcctg gtttgccgac aatctttcgt ccaccagcaa aacgaaaatc ttctcaatca
    11701 actacaccat gctaaacatt ggctggacca tcggtccgcc gctcggcacg ctgttggtga
    11761 tgcagagcat caatctgccc ttttggctgg cagctatctg ttccgcgttt cccatgcttt
    11821 tcattcaaat atgggtaaag cgcagcgaga aaatcatcgc cacggaaaca ggcagtgtct
    11881 ggtcgccgaa agttttatta caagataaag cactgttgtg gtttacctgc tctggttttc
    11941 tggcttcttt tgtaagcggc gcatttgctt catgcatttc acaatatgtg atggtgattg
    12001 ctgatgggga ttttgccgaa aaggtggtcg cggttgttct tccggtgaat gctgccatgg
    12061 tggttacgtt gcaatattcc gtgggccgcc gacttaaccc agctaacatc cgcgcgctga
    12121 tgacagcagg caccctctgt ttcgtcatcg gactggtcgg ttttattttt tccggcaaca
    12181 acctgctatt gtggggtatg tcagctgcgg tatttactgt cggtgaaatc atttatgcgc
    12241 cgggcgagta tatgttgatt gaccatattg cgccgccagg aatgaaagcc agctattttt
    12301 ccgcccagtc tttaggctgg cttggtgccg cgattaaccc attagtgagt ggcgtagtgc
    12361 taaccagcct gccgccttcc tcgctgtttg tcatcttagc gttggtgatc attgctgcgt
    12421 gggtgctgat gttaaaaggg attcgtgcaa gaccgtgggg gcagcccgcg ctttgttgat
    12481 ttaagtcgaa cacaataaaa atttaattca gccttcgttt aggttatctc tgctaatatc
    12541 tttctcattg agatgaaaat taaggtaagc gaggaaacac accacaccat aaacggaggc
    12601 aaataatgct gggtaatatg aatgttttta tggccgtact gggaataatt ttattttctg
    12661 gttttctggc cgcgtatttc agccacaaat gggatgacta atgaacggag ataatccctc
    12721 acctaaccgg ccccttgtta cagttgtgta caaggggcct gatttttatg acggcgaaaa
    12781 aaaccgccag taaaccggcg gtgaatgctt gcatggatag atttgtgttt tgcttttacg
    12841 ctaacaggca ttttcctgca ctgataacgg atcgttgaca cagtagcatc agttttctca
    12901 atg
//
LOCUS       DAJTUI010000100        12682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTUI010000100 DAJTUI010000000
VERSION     DAJTUI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.100"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(99..737)
                     /gene="rutR"
                     /locus_tag="OO464_003227"
     CDS             complement(99..737)
                     /gene="rutR"
                     /locus_tag="OO464_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR6228269.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1025..2116
                     /gene="rutA"
                     /locus_tag="OO464_003228"
     CDS             1025..2116
                     /gene="rutA"
                     /locus_tag="OO464_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR6228270.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2116..2808
                     /gene="rutB"
                     /locus_tag="OO464_003229"
     CDS             2116..2808
                     /gene="rutB"
                     /locus_tag="OO464_003229"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR6228271.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2820..3206
                     /gene="rutC"
                     /locus_tag="OO464_003230"
     CDS             2820..3206
                     /gene="rutC"
                     /locus_tag="OO464_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6228272.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3214..4014
                     /gene="rutD"
                     /locus_tag="OO464_003231"
     CDS             3214..4014
                     /gene="rutD"
                     /locus_tag="OO464_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR6228273.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4024..4614
                     /locus_tag="OO464_003232"
     CDS             4024..4614
                     /locus_tag="OO464_003232"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR6228274.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4625..5119
                     /gene="rutF"
                     /locus_tag="OO464_003233"
     CDS             4625..5119
                     /gene="rutF"
                     /locus_tag="OO464_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR6228275.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5140..6468
                     /gene="rutG"
                     /locus_tag="OO464_003234"
     CDS             5140..6468
                     /gene="rutG"
                     /locus_tag="OO464_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR6228276.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6550..6657)
                     /locus_tag="OO464_003235"
     CDS             complement(6550..6657)
                     /locus_tag="OO464_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228277.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7030..7626
                     /gene="wrbA"
                     /locus_tag="OO464_003236"
     CDS             7030..7626
                     /gene="wrbA"
                     /locus_tag="OO464_003236"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6228278.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7647..7874
                     /locus_tag="OO464_003237"
     CDS             7647..7874
                     /locus_tag="OO464_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR6228279.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7912..9153)
                     /gene="agp"
                     /locus_tag="OO464_003238"
     CDS             complement(7912..9153)
                     /gene="agp"
                     /locus_tag="OO464_003238"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR6228280.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9446..10702)
                     /locus_tag="OO464_003239"
     CDS             complement(9446..10702)
                     /locus_tag="OO464_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR6228281.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10963..11883
                     /gene="cbpA"
                     /locus_tag="OO464_003240"
     CDS             10963..11883
                     /gene="cbpA"
                     /locus_tag="OO464_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR6228282.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11883..12188
                     /gene="cbpM"
                     /locus_tag="OO464_003241"
     CDS             11883..12188
                     /gene="cbpM"
                     /locus_tag="OO464_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR6228283.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12281..>12682)
                     /gene="torD"
                     /locus_tag="OO464_003242"
     CDS             complement(12281..>12682)
                     /gene="torD"
                     /locus_tag="OO464_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6228284.1"
                     /translation="RDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVE
                     AGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEF
                     AARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3029 a         3238 c         3196 g         3219 t
ORIGIN      
        1 cgctgagtgt gaataccgct gccgctggcg gtaacgccag tttaatgacg atcggttaaa
       61 caggtagccg gaggatgtta caacctcctc cgtcatcttt aacgtggtcg aatcccctca
      121 ataataatcc gctgcacgtt ttcaaccgtt tgattgaaaa atacctcatc gcgcaacgtc
      181 gcgcctgtca ccgcctccac ctgaggggcg aaatcggcgt aatgttgagt ggaagcccaa
      241 atcataaaaa tcaaatgctg cggatcaatc ggcgcgagtt tgccgctttt gacccaaccg
      301 gcaatcagcg ccgatttctc atcaattaat gccttcaaat cgcccgtcag ttcatccatt
      361 aacaacggcg cgcctgccag catctccata cagaacaggc gcgaagcctg cggataatcg
      421 cgtgagactt ccagcttcag acggatgtac tcttttatcg ccgccagcgg ggcgaaatct
      481 tcacgaaacg cttttaacgg tgccagccag atatcgagaa tctgccgcag cacggcaata
      541 tacagcgcct cttttgacgg gaagtaatac agcagattgg tttttgaaac acccgccaac
      601 tctgcgatct gctccagcct tgtgccgtga aaaccgaatt gtgaaaaagt gtccagtgct
      661 gcgctaagaa tcgctttttt cttcgcgctt actgcgcgcg aacgtttacc cgttgttttc
      721 actgcgcctt gcgtcatgcg ctctcccctc ttgtagattg gtaacaggat agcaaaaccc
      781 ccgcccggcc tcgaccttct gcactctcat cgcgcacgga tgcatgtttt atgtgcaact
      841 gttttgaccg tttagtccac tttttaccag atattttaga taacacaatg ctaacacatt
      901 aataatattc actttttaaa aactggcatc cgctttgcga acaagccaat acgcggctga
      961 aaaggatatg caggatgcag cgccccgtct gactttcact ttacgagacg aagagaggtt
     1021 gatgatgaaa attggcgtat tcgtacctat tggcaacaac ggctggctca tttcgaccca
     1081 cgcgccgcag tacatgccga cctttgaact gaataaagcc attgcgcaaa aagcggagca
     1141 ttaccatttc gatttcgccc tgtcgatgat caaactgcgt ggctttggcg gcaaaactga
     1201 gttctgggat cacaaccttg agtcgttcac cttgatggcg ggtctggcag ccgtgacctc
     1261 gcgcattcag atttacgcca ctgctgccac cttaacgtta cctccggcaa tcgtcgcccg
     1321 tatggccgca accatcgact ccatctctgg cgggcgtttt ggcgtcaacc tcgtgactgg
     1381 ctggcaaaag cccgagtatg agcagatggg tatctggcct ggcgatgact atttctcccg
     1441 tcgttacgac tatctcaccg aatatgttca ggtgctgcgc gacctgtggg gcacggggaa
     1501 aagcgatttt aaaggcgatt ttttcaccat gaatgattgt cgcgtcagtc cgcaaccgag
     1561 tatccccatg aaagtgatct gcgccgggca aagcgacgct ggcatggcgt tctccgccca
     1621 gtatgccgat ttcaacttct gtttcggcaa aggcgtaaat acacccacgg ctttcgcccc
     1681 gaccgctgcg cggatgaaac aggccacaga gcaaaccgga cgcgacgttg gctcttatgt
     1741 gttgtttatg gtgattgccg atgaaaccga cgatgccact cgcgccaaat gggaacacta
     1801 caaagcgggc gcggatgaag aggcgttaag ctggctaacc gaacaaagtc agaaagatac
     1861 ccgctccggt actgacacca acgttcgtca gatggccgat cccacttcgg cggtaaacat
     1921 caatatgggg acgttagtcg gttcttacgc cagtgtcgcg cgcatgttag atgaagtcgc
     1981 aagcgtgcct ggtgcagaag gcgtgctgtt gaccttcgat gattttctgt cgggaatcga
     2041 aaccttcggc gagcgcattc aaccactgat gcagtgccgc gcccatctct ctgcgctgac
     2101 tcaggaggtg gcatgatgac aaccttaacc gctcgaccgg aagccattac cttcgatccg
     2161 cagcaaagtg cgcagatcgt ggtggatatg caaaacgctt atgccacgcc aggcggctac
     2221 ttagatctcg ccgggtttga tgtctcaacc actcgcccgg tcattgccaa cattcaaacc
     2281 gctgtgaccg cagcgcgagc ggcagggatg ttgatcatct ggtttcaaaa tggctgggat
     2341 gaacagtatg tcgaggctgg cggccccggc tcaccgaatt ttcataaatc gaacgccctg
     2401 aaaaccatgc gtaagcagcc gcagctgcag gggaaattgc tggcgaaagg ctcctgggat
     2461 tatcaactgg tggatgaact ggtgccgcag cctggcgata ttgtgctgcc gaagccgcgc
     2521 tacagcggtt tcttcaatac gccgctggac agcattttac gcagccgtgg aatacgccat
     2581 ctggttttca ccggtatcgc taccaacgtc tgcgtcgaat cgacgctacg cgatggcttt
     2641 tttctggagc atttcggcgt ggtgctggaa gacgcaacac accaggcggg gccggaattt
     2701 gcacagaaag ccgcgttgtt caatatcgaa accttttttg gctgggtcag cgacgtcgaa
     2761 acgttctgcg acgcgctttc tcccacgtcc tttgctcgta tcgcttaagg agtttagcga
     2821 tgccaaaatc cgtaattatt cccgctggca gcagcgcacc gctggccccc ttcgttcccg
     2881 gcacgctggc tgatggcgtg gtgtatgtct ccggtacgct ggcttttgat caacataata
     2941 acgtgctgtt tgccgatgac ccaaaggcgc aaacccgcca cgttctggaa actatccgca
     3001 aggtgatcga gacggcgggt ggcacgatgg cggatgtgac cttcaacagc atctttatta
     3061 ccgactggaa aaattacgcc gcgattaacg aaatctacgc cgagtttttt ccgggtgata
     3121 aaccggcgcg attctacatt cagtgcggac tggtaaaacc tgacgcgctg gtggaaatcg
     3181 ccacaattgc gcatatcgcc aagtgaggcc gcgatgaaac tttcactctc atctccccct
     3241 tatgctgatg cacccgtagt ggtgttgatt tcgggtcttg gcggtagcgg cagttactgg
     3301 ttaccgcaac tggcggtgct ggagcaggag tatcaggtag tctgttacga ccagcgcggc
     3361 accggcaata atcccgacac actggcagaa gattacagta tcgcccagat ggcagcggaa
     3421 ctgcatcagg cgctggtagc cgcagggatt gagcgttacg cggtggtcgg ccatgcgctc
     3481 ggtgcgctgg tggggatgca gctggcgctg gattatcccg cgtcggtaac tatgctggtc
     3541 agcgttaacg gctggctacg aataaacgcc catacgcgcc gctgttttca ggttcgcgaa
     3601 cgattactgt atagcggcgg cgcgcaagca tgggtggaag cgcagccgtt gttcctctat
     3661 cccgccgact ggatggcggc ccgcgcacct cgcctggagg cagaagacgc gctggcactg
     3721 gcgcattttc agggcaaaaa taatttacta cgtcgactta acgccctcaa acgcgctgac
     3781 tttagtcacc atgcggatcg catccgctgc ccggtgcaaa tcatctgcgc cagtgatgat
     3841 ctgctggtgc caacagcatg ttccagtgaa cttcatgccg ccctgcccga tagccagaaa
     3901 atggtgatgc cctatggcgg acacgcctgc aacgtgaccg atcccgaaac gtttaatgct
     3961 ctgttactca acgggcttgc cagcctgtta catcaccgtg aagccgccct gtaaggaatt
     4021 gctatgaacg aagccgttag cccaggtgcg cttagcaccc tgttcaccga tgcccgcact
     4081 cacaacggct ggcgggagac acccgtcagc gatgagacgt tacaggagct ttatgccctg
     4141 atgaaatggg ggccgacatc agctaactgt tctccggcac ggatcgtgtt tatccgcact
     4201 gcagaaggaa aagaacgtct gcgcccggcg ctttccagcg gcaatctgca aaaaaccctg
     4261 accgcgcccg tcaccgctat cgtcgcctgg gacagtgaat tttatgaacg gttaccacta
     4321 ctgtttcccc acggtgatgc ccgcagttgg tttacctcca gcccacaact tgccgaagaa
     4381 acagcgtttc gcaacagttc catgcaggcg gcttatctga tcgtcgcttg ccgtgcgctg
     4441 ggactggata ccggcccgat gtcgggtttt gaccgtcaac acgttgacga cgcctttttt
     4501 gcgggcagca cgctgaagag caatctgctg attaatatcg gctatggcga tagcagcaaa
     4561 ctttttgcgc gcctgccacg tctgtccttt gaagaagcct gcgggctgtt gtaaggagca
     4621 aactatgaac atagttgatc aacaaacctt tcgcgatgcg atgtcctgta tgggcgcggc
     4681 ggtcaatatc atcaccacgg acggtccagc cgggcgcgcc gggttcaccg ccagcgccgt
     4741 ctgcagcgtg accgacacgc cgcccacatt actggtgtgc ctgaatcgtg gggcgtccgt
     4801 ctggccggta ttcaatgaaa atcgaacgct gtgtgtaaat acactgagcg ccgggcagga
     4861 gccgctttca aatctttttg gcggcaaaac gcccatggaa caccgctttg ccgccgcccg
     4921 ctggcagacg ggtgtgaccg gatgcccgca actggaagag gctctggttt cgtttgactg
     4981 ccgtatcagc caggtggtga gcgtcggcac ccacgacatt ctgttttgcg ccatcgaagc
     5041 gattcatcgt cacaccacac cctacgggct ggtgtggttc gatcgcagtt atcacgcgct
     5101 gatgcgccct gcttgttaac ctccttaagg agacagctga tggcaatgtt cggttttcct
     5161 cactggcagt taaaatcgac ctctacagag agcggcgtgg tcgcgccgga tgaacgattg
     5221 ccgtttgctc agacggcagt aatgggcgtt caacacgcgg tggcgatgtt tggcgcgacg
     5281 gtgttaatgc cgattctgat ggggctggat cccaatcttt ccattttaat gtcggggatc
     5341 ggcacgttac tgtttttctt tattaccggc gggcgcgtcc ccagttatct cggctccagc
     5401 gcggcttttg tcggcgtggt gattgccgcg acgggcttta acggtcaggg cattaacccg
     5461 aatatcagca tcgcgctggg cgggattatc gcctgtgggc tggtttatac cgtcattggc
     5521 ctggtggtga tgaaaattgg tacacgctgg attgaacgac taatgccgcc ggtggtgacg
     5581 ggtgcggtgg tgatggcgat tggcctgaac ttagcgccta tcgcagtgaa aagtgtatcg
     5641 gcttcggcct ttgatagctg gatggcggta atgacggtgc tgtgtattgg gctggtggcg
     5701 gtgtttaccc gtggcatgat ccagcggtta ttgattctcg taggattaat tgtcgcctgt
     5761 ctgctgtatg gcgtgatgac caacgtttta gggctgggaa aagccgtcga ttttactctc
     5821 gtcagctatg ctgcctggtt cggtctgccg catttttcca ctcctggttt taatggccag
     5881 gcgatgatgt tgattgcgcc cgtggcggtg attctggtgg cggaaaactt aggtcatctc
     5941 aaggcggtcg ccgggatgac cggacgcaat atggacccgt atatggggcg agcattcgtc
     6001 ggtgatggtc tggcaacgat gctttccggc tctgtcggcg gtagcggcgt caccacctat
     6061 gctgaaaata tcggtgtgat ggccgtgaca aaagtctact cgacgctggt gtttgttgca
     6121 gctgccgtca tcgctatgct gttgggcttt tcgccgaaat ttggcgcgct gatccatacc
     6181 attccggccg cggtgattgg tggtgcatca attgttgtgt tcgggctgat tgccgtcgca
     6241 ggcgcaagga tatgggtaca aaaccgtgtc gatttaagcc agaacgctaa tttgattatg
     6301 gtcgcagtaa ccttcgtgct gggcgcgggt gattttgctc tcacgcttgg cggttttacg
     6361 ttgggaggga tcggtacagc aacctttggc gcgattttac tcaatgcgtt attaagcaga
     6421 aggttggttg acgttccacc gccagaagtt gttcatcagg agccataaac agaaaaccgc
     6481 cgggttaccg gcggtgatga ctttttgacg agcttaagaa aaaaatgcat tcgtcattga
     6541 ttagcccggc tagttgtcgc ttttgccgtg actgctcttg ccaccttttt tacctgcttc
     6601 tgatgctctt tcgcggtctt ctgcaaaatt gccggaaccg cctcgatggt ttgccattac
     6661 tgacctccgt ctgggtatag acatcatatt gcatagaatt aacgcggcgt atggcggctg
     6721 ccatattttc gctacgtggg actaagctta gtgaactgac gcggacagac ccgcgtttgg
     6781 taatatcctg caacagcttc ttagcgagaa tagatatcat ctccaataat tatccataag
     6841 ccgctcttat gattaaaaaa acgcgatgag ataaaaatgt gtcattttgc gacaaaatta
     6901 cgtgcttgtg aaagttgtta taaatcaaat aagtggttgt gaaatttgca ctctgaaaag
     6961 gacgtcttat ctttaaataa gtggtagcga atcgctacgg aatagagata acacgaggag
     7021 tggttagaaa tggctaaagt tctggtgctt tattattcca tgtacggaca tattgaaacg
     7081 atggcacgcg cagtcgctga gggtgcaagc aaagtggatg gcgcagaagt tgtcgttaag
     7141 cgtgtaccgg aaaccatgcc gccgcaatta tttgaaaaag caggcggtaa aacgcaaact
     7201 gcaccggttg caaccccgca agaactggcc gattacgacg ccattatttt tggtacacct
     7261 acccgctttg gcaacatgtc cggtcaaatg cgtaccttcc tcgaccagac gggcggcctg
     7321 tgggcttccg gcgcactata cggaaaactg gcgagcgtct ttagttccac cggtactggc
     7381 ggcggtcagg aacaaaccat cacatccacc tggacgaccc ttgcgcatca cggcatggtg
     7441 attgtcccca ttggctacgc agcgcaggaa ttatttgacg tttcacaggt tcgcggcggt
     7501 acgccgtacg gcgcaaccac catcgcaggc ggtgacggct cacgccagcc aagccaggaa
     7561 gaactgtcta ttgctcgtta tcaaggggaa tatgtcgcag gtctggcagt taaacttaac
     7621 ggctaatctt caacaggagg atacgcatgc caactcaaga agcgaaagct catcacgtcg
     7681 gtgaatgggc atctctgcgc aatacgtcgc cggaaatagc cgaggccatt ttcgaagtcg
     7741 ccgggtacga cgaaaaaatg gcagaaaaga tttgggaaga aggtagtgat gaagtgttag
     7801 tcaaagcctt tgctaagacc gataaagatt cgcttttttg gggcgaacag accatcgaac
     7861 gtaaaaacgt ttaaccagcg actcccccgc ttctcgcggg ggagttttct gttatttcac
     7921 cgcctcattc aacacgctat caaacttatc catcgggcag aaaccgttgg catctatcgg
     7981 gcaaccgctt aattccagcg tcacgcgctg cgcaggtgcc tgcagggtta acgcatcggc
     8041 attacgtaac tgttccgcac tctggtacac atattcaatt ttcatcaaat cgcgattggc
     8101 tttgctgtca tgccaacgct ggaaaacgat tttgccgcca atcggcgtgc gttcgttctg
     8161 gtcatgcaac tgatacggtt tgaaatccag cgccgttaac agagaggcta tgttggagtc
     8221 gtgcccaact aacaccgtga ttttcggagc actggcgcga tcggtgacca gggctttgtc
     8281 gatataactg accagcggtt tcgcaacatt gcgcgccact tccggtgagg taaacagact
     8341 gtcctggtaa ccgtttttca gcttcgacaa taccttccac tgctggtcag atttgatttc
     8401 tccccaggcc acctgatcca tcggaaaacc ttcgtagtat tgcaaagtaa acgcatctac
     8461 cagcgagttg ccgactttca gcggcccgga aacacctggt tcttgttgat acttggcgct
     8521 aaaggtattt ttgccatcca ccagcgaaca ctgttgtttc tctttacagg caggggaatc
     8581 tttatagtta acgatttttt ccagtagctg gtagctgtcg gtaagctgga gtttgctgag
     8641 ctctttctcc attgccgcca ccgcctgttc actgaatgcg gcggaatcat cggtgatcat
     8701 cgggttaaag gttgggtcca tagtgcccat tttttcctgg tgatgcacag gaatatcaca
     8761 cccagggaat gcaccagtaa taaagaactg ggcggtagcg acggtacgtt gcagactatt
     8821 ggcataggca taaacggtgt ccggcggcgg gcattccccc gatttcacta tcccctgctc
     8881 tgccagccat tcacgcatgt aatggcccat atacacttcg agcacgccac ctttggtggt
     8941 gagttgccca ccggggacgt cccattcggg ccatttattc ggcgtcgact gctccagcac
     9001 actgccattg ttcgccagcg gcgcacgtaa gttatggcgg ctcatcatga gcacttgctg
     9061 tagctgatag ccttccggta cggtttgtgc ctgagcattt gaagcgagta aaactatccc
     9121 tgccacagtt gcggcgatta gcgttttgtt cattcctggc acctcttttg ttatcaatca
     9181 ttaaagagtg tgacagaaat atgacttttt tccgggaaca ggttcgcaaa atggcttgtt
     9241 gaacgcctgt ttttctatat acatcttcaa aaacaggcaa ggtttgacgc aaactatgcc
     9301 tgattagccc ctgcctcgcg tacgccgctg ataattgcac ctgtggctaa acagaatatc
     9361 tgcggcgtta ccaatacaga gacaatattt accaataaaa aagtgtgaga ctaataacaa
     9421 tggtttttag catatgcctt tgccattaag ttgcataacg atttaaatag tcaagttgcg
     9481 cattcatttc cttatttttt aacttataag cctgagaaaa aagttcagga gggaggtaaa
     9541 catcataatg attgccagtg tttgttaaat atccatactc aagatcttca tctgttgaaa
     9601 aggattcatt tcccaaggag attgatgcca cattactatt aacaataaat aatgagcaaa
     9661 tgctatctga aagaacagca agaaaattct tcaccgcttt aatcctatcc gacgtgtcgg
     9721 tatctaaagc aagcaaattc ttgttatcat taaatataaa ctcgggtaaa tgcagtatgc
     9781 attttttgtc attcaagcaa ctatcaatga attgtttttt aaaaaacaga aagctccatg
     9841 acataaaact acccacaccc tgaattttgt tgtcagattg cagatcaacg ccaaataatg
     9901 tctttacatc ttccctgaga ttatagaatg ccgtatcaac aagagcctcg atcgtttgtt
     9961 tatcaacaat tttaataaac tcttcgtata cttgcttttg cgtcttatgg ctgcccagcg
    10021 cccatgaata ctcatgttct tttaaaacca gtatggctct taacagacaa tatccatcgc
    10081 catctactgg agcgtatttt aatccatatg ttttattaat ttcactctga ataaaagata
    10141 tgccagacaa atcaatacca gtatctaaat cagatattcc cggcatattt atctgttctg
    10201 gctggatact catattttca ttatctaaac aatactcaga aaaaacatca tcacacgcat
    10261 taatgttttc aatatattcc ataaagccat accaataata acctttaaaa tctgtgatag
    10321 actctgaacc tctgcccggc aaggacactc taacaatatc aacatcttga acatattgta
    10381 tagtatatgt aatacctcct gaaacccctg aataaatttg tgtatcatta gtacgcaatg
    10441 ttctcataaa taatgataga tcattatcat tctgcatttc ctcaaattct ttttctagtg
    10501 attcacattc cagacatgga gtatcctctt catttacttt acgtctgagt tcattgtaca
    10561 accgcataaa acacaccagt gtgttttcaa aaagcgtgat tgaatatgtt tgattttttt
    10621 cgtactcatt ttttatatcg ttccaggcct gtttcaaata attatttgca gtgcaactaa
    10681 ttccatgtat attactaccc atatatagcg tctataaaat ttaataaata atgacgccct
    10741 agttaaactt aaagtgcctg gttcaactat caaaaatcgc tcaccctttt tcacctgttt
    10801 aaaatatgtt cagcaaccca tcttgatggc gacctcctct ccgcgatgat ttcaataaca
    10861 tattctgtgt tggcatatga aattttgagg attaccctac acttatagga gttaccttac
    10921 aggggttcct tcaatttgtg ttgatttacg cgagataacg ctatggaatt aaaggattat
    10981 tacgccatca tgggcgtgaa accgacggac gatctcaaga caatcaagac cgcctatcgt
    11041 cgactggccc gtaaatacca tcctgatgtc agcaaagaac cggatgccga agcccgcttc
    11101 aaagaggtcg ctgaagcctg ggaagtgtta agtgatgaac aacgtcgcgc tgagtatgat
    11161 cagatgtggc aacatcgcaa cgatccgcaa tttaaccgtc agttccatca tggcgacggt
    11221 cagagtttta atgccgaaga ttttgacgat atcttctcgt caattttcgg tcagcatgcc
    11281 cgccagagcc gtcaacgccc cgccgcacgt ggccacgata ttgaaatcga agtggcggta
    11341 ttcctcgaag aaacgcttac tgagcataag cgtaccatca gctataacct gccggtttat
    11401 aacgcctttg gcatgatcga acaggaaatc ccgaaaacgc tgaatgtgaa gatcccggca
    11461 ggcgtcggca atggtcaacg catccgcctg aaaggccagg ggacgccggg cgaaaacggc
    11521 ggtccaaatg gcgatttgtg gctggtgatt catattgcgc cacatccgct gttcgatatt
    11581 gtcggccagg atctggaaat tgtggtgccg gttagcccgt gggaagcggc gctgggtact
    11641 aaagtcaccg ttccaacact gaaagaaagc attttgctga ctatcccgcc aggcagccag
    11701 gccgggcaac gattgcgcgt taaaggcaaa ggtctggtga gcaaaaaaca gaccggcgat
    11761 ctgtatgcgg tactgaaaat cgtgatgccg ccgaaaccgg atgaaaacac tgccgcgctg
    11821 tggcagcaac tggcagacgc ccagtcgtct tttgatccac gtaaagattg ggggaaagca
    11881 taatggctaa tgttacggtg acttttacta ttaccgaatt ttgcctgcat accggcatct
    11941 ctgaagagga gttgaatgaa attgtcggtt tgggggtggt tgaaccgcgt gagatccagg
    12001 aaacaacctg ggtatttgac gaccatgccg ccattgtggt gcaacgcgcg gtacgcctgc
    12061 gtcatgaact ggctctggac tggccgggga tcgcggtggc gctgacgtta atggatgata
    12121 ttgcgcacct gaagcaggaa aaccgcctgc tacgccagcg gctttcccgg tttgtagctc
    12181 atccgtgatg tgtgtttgcc ggatgtgatg acgcatccgg caactgcgcc ataatgcctg
    12241 atgcgacgct aaacgcgtct tttcaggcgc ataaacgacg ttatctgttt tggtggtcgc
    12301 actccactaa caccagcaat aactggctta gtgccgcgta aaaaccaaag ctgtcatact
    12361 gtcgacaacg cgcagcaaac tctggtaacc attgccgcag cgccgtcagt gttttttgcc
    12421 gcaaactgtc gattcttcgc gcaggaacgg tcccctctcc cagcgaaaaa tgcagataac
    12481 tcaacaactc gagatagatc gccagatgat ctgccggttc gttgaaattg ccactggttt
    12541 ccatccctgc ctcaactaac aagcgtttaa tctcttgctc gtcctgtttg taggccgatg
    12601 catacggcag cgccgcttgt ttgtcggtca tcagaaacag gccgcaaaag tcagcggcca
    12661 gttccagacg ggcatcgtca cg
//
LOCUS       DAJTUI010000101        12629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTUI010000101 DAJTUI010000000
VERSION     DAJTUI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.101"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(139..858)
                     /locus_tag="OO464_003243"
                     /pseudo
     CDS             complement(139..858)
                     /locus_tag="OO464_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(874..1206)
                     /gene="cusF"
                     /locus_tag="OO464_003244"
     CDS             complement(874..1206)
                     /gene="cusF"
                     /locus_tag="OO464_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR6228285.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1230..2612)
                     /gene="cusC"
                     /locus_tag="OO464_003245"
     CDS             complement(1230..2612)
                     /gene="cusC"
                     /locus_tag="OO464_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR6228286.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            2769..3452
                     /gene="cusR"
                     /locus_tag="OO464_003246"
     CDS             2769..3452
                     /gene="cusR"
                     /locus_tag="OO464_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR6228287.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3442..4884
                     /gene="cusS"
                     /locus_tag="OO464_003247"
     CDS             3442..4884
                     /gene="cusS"
                     /locus_tag="OO464_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR6228288.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5034..7271
                     /gene="nrfB"
                     /locus_tag="OO464_003248"
     CDS             5034..7271
                     /gene="nrfB"
                     /locus_tag="OO464_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR6228289.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7258..10226
                     /gene="nfrA"
                     /locus_tag="OO464_003249"
                     /pseudo
     CDS             7258..10226
                     /gene="nfrA"
                     /locus_tag="OO464_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10227..11117
                     /locus_tag="OO464_003250"
     CDS             10227..11117
                     /locus_tag="OO464_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR6228290.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11300..12061
                     /gene="envY"
                     /locus_tag="OO464_003251"
     CDS             11300..12061
                     /gene="envY"
                     /locus_tag="OO464_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR6228291.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12107..12183)
                     /locus_tag="OO464_003252"
     tRNA            complement(12107..12183)
                     /locus_tag="OO464_003252"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12147..12149),aa:Arg,seq:tct)
     gene            12426..>12629
                     /locus_tag="OO464_003253"
     CDS             12426..>12629
                     /locus_tag="OO464_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR6228292.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3081 a         3287 c         3212 g         3049 t
ORIGIN      
        1 acagcgcaca gtaaacaact atatttgacg ttcgtagaca tactcaggtg aaaaaaaggt
       61 tgtttttcga gttataacag acagttaatt aactacagtg gacaatgcta gacaatgaca
      121 gacacagata agcaacctac cttcctcttt cgcgaccacg ttatctttgg cgatattcat
      181 tcccgcgcgc agatcaaacg cggtgatcac gccatcaatg ggcgctttga gcgtaaagcg
      241 agtctggatt ttttgcgtgg cgatcaggtg gcgaatatcc gcctccggca ttcccgccag
      301 tcgcagccgc tcaagaatgc cttcagtctg ggtcgccgta ccgccggttt cgcgcaacag
      361 taaatactca ctctgcgctt ccacccagtc gggaatggtc aggtcgagaa gcggtgtgcc
      421 cttttgtact ttatcgccca cggtaagcgg atacaccttg tcgataaacc cggcagcgcg
      481 agcctgcaca atggcatact gatactcgtt gtaactgaca ttcgtcggga aactctgggc
      541 aaaagtcagc ggcccgcgcg tgacggtagc cgttttcacc cccagattct gcgtctgagt
      601 cgggtcaatg cgcacaccag acgcagaact ctcttcatcg gcatatttcg gcaccagatc
      661 catatccata aacggcgatt tacctggttt atcgaaccgc gtattgggat acatcgggtc
      721 gtaccagaat aagattttac gttctgcggt cggcattttt tctgcgtgcg gttccgcctg
      781 gtcaaaccag gtaaaacctg ccgctgaaat aataccgccc gcgatcatgc tgccgataat
      841 aagcgcgatt tttttcatct cattaaacct gggttactgg ctgactttaa tatcctgtaa
      901 taaagaaagg ttgccctgct ggacaaaatt aaacgccact ttgtcgccgg ttttaatttc
      961 actcattttc gtctgcgggg tgatggtaaa gcgcatggtc atctccggcc agttgacggc
     1021 agcaatcgga tcgtgatgga tggtgatttt tttgctttcc agatcaacac cttttaccac
     1081 accggtggca ctaatgacct gtggttgtgc ttcgctcatg gtttcatgat gatgttcgtt
     1141 agcctgggca ttaaagccaa taacggtaaa cagactgaac attgcgactt gcagtgcttt
     1201 tttcatttat tctctcctgg agttaaaaat tattgctgcc aaccgccccc taaggcggta
     1261 tacaaataaa tttcgttaac ctgacgggca taattcaggt caagtaaagt ttgtcgggtt
     1321 gcaaataaag aacgctcggc atccagcact tccagataac ttaccgcgcc gtgctgatat
     1381 aatgcccgcg cccgttgcag ggtaatttgc agcgacgcca gataacgctg ctgggcgctg
     1441 atttgatcgt ccaggctttg acgtaatgcc agcgcatctg ccacctcttt aaaggcgttc
     1501 tggattttct gttcataatt caccaccgac tgctgctggc gaatttcggc gatatccaga
     1561 ttggcctggt tgcgtccggc attaaaaatg gggatctcaa ttttaggaat aaaattccac
     1621 atcccgctgc tagcgttaaa taatgacgac agatcgctgc tggcggttga tattccgctg
     1681 gtcaggctga tagacgggaa aaatgccgca cgcgcagcac caatattagc attagccgcc
     1741 attaacgcgt gttcagcttc cataatatcc gggcgctgca ataagatttg cgacgacaag
     1801 cccgccggta atttaacgct ttgcaggctg ccgctgttta ctgtctgcgc ttgcggtagc
     1861 ttgccgtagc ttcccaataa cagttgcaat gcattattcg cctgcgccag ttccccctga
     1921 cgtttagcga tgtcactgcg ggtactttct atcaccccgc gagcctgttc cagcgccaga
     1981 acattgctgc taccggtcaa cagttgtttt tcgacaaacg catatgactg ctgataatta
     2041 cgcagcgttt cttcggctat ttgcaattgc gcatacgcca gctgctgatt gaaatagctt
     2101 tgcgcgacat tagaaaccag caaaatatgc accgcgcgct gggcttcctc agtggctaaa
     2161 taattttggc gctcggcttc gctcatgttc tttaagcgac cgaaaaaatc gagatcaaag
     2221 ctggcgttaa ggccagtcga gaactcccgc gtcgtggctg aatcgccttt aagattgccg
     2281 ctccagctgc cgctgccctc gccattgagc tgtgggtagc ggtcggcatc ggtcagacga
     2341 tattgcgccc gtgcctcctg cactttcagc gtcgccatgc gcaaatcccg gttattcacc
     2401 agcgcctcgc taatcagcgt cttcacctga ttatcaacaa aaaaggtgcg ccagcccacg
     2461 ttctggtagt tatccgctgc gttaaccagg ccgttctggc tgagtgagaa ctgctgcggt
     2521 acgggcatta ccggacgctg ataatccggt gccagtgaac aaccagttag cgcaagggcc
     2581 acacaaaatg gcagaagttt acaaggagac ataggctcat aatttctggt gattttatgt
     2641 cgccaacttt actcgccagg ctctgatttt ccggtgacag gaaaatgaca aaattgtcat
     2701 tttgccaata agcgattgcc atctgatccc gctactctag aattgcccgg gcaacatgcg
     2761 gaggaaatat gaaactgttg attgtcgaag atgaaaagaa aaccggagaa tacttgacca
     2821 aagggttaac cgaagccggt tttgtggtcg atttggccga caacgggctg aatggctacc
     2881 atctggcgat gaccggtgat tatgacctga taatcctcga tattatgctg ccagacgtga
     2941 acggctggga tatcgtgcgc atgttgcgct ccgccaataa agggatgccg attctgttgc
     3001 ttaccgcgct tggcaccatt gaacatcgcg tcaaggggct ggagttgggg gcagatgact
     3061 acctggtgaa accgttcgct tttgctgaac tgctggcgcg ggtgcgcact ctcctgcggc
     3121 gcggggcggc ggtgattatc gaaagtcagt ttcaggttgc cgatctgatg gtcgatctcg
     3181 tcagccgcaa agtcacccgc agcggcacgc gcatcactct gaccagtaaa gaatttactc
     3241 tgctggagtt ttttctccgc catcagggcg aagtgctgcc ccgctcgctt atcgcctcgc
     3301 aggtatggga catgaatttc gacagcgaca ctaacgccat tgatgtggcg gtgaagctgc
     3361 tacgcggcaa aatcgacaac gactttgagc cgaagctgat tcaaaccgtg cgcggcgtgg
     3421 gttacatgct tgaggtgccg gatggtcagt aagccatttc agcgcccgtt ttcgctggca
     3481 acccgcctga ccttttttat cagccaggcc accatcgcgg cgtttttcgc ctttgcatgg
     3541 atcataatcc actcagtaaa agtgcatttt gccgagcagg acattaatga tttaaaagag
     3601 attagcgcca cccttgaacg ggtactaaat caccctgacg aaacgcaagc ccgacgctta
     3661 atgacgctgg aagatatcgt cagtggttat tccaacgtgt tgatttccct ggcagatagc
     3721 cacggcaaaa cggtgtatca ctcccccggt gcgcctgata tccgcgagtt tgcacgtgat
     3781 gccatacccg ataaagacgc gcggggtggc gaggtgtatc tcctttccgg cccgacgatt
     3841 atgatgccag gccacggtca cgggcatatg gaacacagca actggcggat gattaacttg
     3901 ccggttggcc cgttggtgga cggcaaaccg atttatacgc tctacatcgc gctgtcgatc
     3961 gattttcatc ttcattacat aaatgatttg atgaataaac ttattatgac cgcatcggta
     4021 atcagcatcc tgatcgtctt tatcgtgctg ttggcggtac ataaaggtca cgcgccgatc
     4081 cgcagcgtca gccgtcaaat ccagaatatc acctcgaaag atctcgacgt tcgccttgac
     4141 ccgcagaccg tgccgattga gctggaacag ctggtactgt cgttcaacca tatgatcgag
     4201 cgtattgagg atgtctttac tcgccagtcc aatttctcag cggatatcgc ccacgaaatt
     4261 cgcacaccaa ttactaatct cattacgcaa acggaaatcg ccctcagcca gtcgcgcagc
     4321 cagaaggagc tggaagatgt gctctactct aatctcgaag agctgacgcg aatggcgaaa
     4381 atggtcagcg atatgctgtt tctcgctcag gccgataaca accagctaat ccccgaaaag
     4441 aaaatgctca acctggcgga tgaagtcggc aaagtgttcg attttttcga ggcgctagcg
     4501 gaagatcgcg gcgtggagtt gcggtttgtt ggcgacaaac gtcaggttgc gggcgatccg
     4561 ttgatgctgc gtcgggcgct aagcaacctg ctttctaatg cccttcgtta tacgccaacc
     4621 agagagacaa ttgtagtacg ctgtcagacg gtcgatcacc aggtgcaagt ttccgtcgaa
     4681 aaccccggta cgcccattgc gcccgagcac ttaccgcgat tgtttgaccg tttctatcgc
     4741 gttgacccct cccgccagcg aaaaggtgaa ggtagcggta ttgggctggc gatagtgaaa
     4801 tcgattgttg tcgcgcataa aggcacggtt gcggtaacgt cagatgcgcg ggggacaagg
     4861 tttgttatca cattacccgc ttaattattc agcaaacggc aacttttata accagtgtaa
     4921 aaataacgtg ccgcaatagt catacatatt aatggtaaaa agctgtcaca attcataaaa
     4981 aaccttaata tacgccaccc taaacataac cagcgttaat gtaaggtttt tgtgtggact
     5041 ggcttcttga tgtttttgct acctggctct acggcttaaa agtaatcgcg ataacgttag
     5101 cggtcatcat gttcatcagc gggctggacg atttttttat tgatgtcgtc tactgggtac
     5161 gccgcattaa acgcaagttg agtgtttatc gccgctaccc gcgaatgagt taccgcgaac
     5221 tgtataaacc agatgaaaaa ccgttagcga ttatggttcc ggcgtggaat gaaacgggcg
     5281 tcatcggcaa tatggccgag ctggcggcga ccacgctcga ctacgaaaac tatcatatct
     5341 ttgttggcac ctaccccaac gaccccgata ctcaacgtga tgttgacgaa gtgtgcgctc
     5401 gctttccgaa cgtgcataag gtagtctgcg cgcgtcctgg tcccaccagc aaagccgact
     5461 gcctgaacaa cgtgctggac gccatcaccc agtttgaacg tagcgccaat ttcgcttttg
     5521 ctggttttat tctgcatgac gccgaagatg tgatttcacc gatggaattg cgtctgttca
     5581 actatctggt cgagcgtaaa gatctgattc agatcccagt gtatccgttt gaacgcgaat
     5641 ggacgcactt caccagcatg acttacattg atgagttttc agaactgcat ggcaaagatg
     5701 ttccggtgcg tgaagccctc gccggacaag tgcccagcgc aggcgtcggc acctgtttca
     5761 gccgccgcgc cgtgaccgca ctgttagctg acggtaacgg tattgctttc gacgtgcaga
     5821 gtcttactga agattacgac attggcttcc gcctgaaaga aaaaggtatg acggaaattt
     5881 ttgtccgttt tccggtggtg gacgaagcca aagaacgcga gcagcgtaaa tttttacagc
     5941 acgcacggac gtcaaacatg atctgcgtgc gcgaatattt ccccgatacc ttttcgactg
     6001 cggttcgaca aaaatctcgc tggatcatcg gcattgtttt ccagggcttt aaaacccata
     6061 aatggacctc cagcctgacg ctgaactact ttctctggcg cgaccgcaaa ggggcaatca
     6121 gtaactttgt cagcttcctc gcgatgctgg tgatgatcca gcttttgctg ttgctggcgt
     6181 atgaaagttt gtggcccgat gcctggcatt tcctttctat ttttagcggc agcgcatggt
     6241 taatgaccct gctgtggcta aactttggct tgatggttaa ccgcatcgtg cagcgagtga
     6301 ttttcgtcac tggctactac ggcctcacgc agggcctgct ttccgtcctg cgtcttttct
     6361 ggggcaacct gattaacttt atggctaact ggcgcgtgct gaaacaggtt cttcagcacg
     6421 gcgatccgcg tcgcgtcgcg tgggataaaa caacgcatga cttccccagc gtcactggcg
     6481 atacccgctc gttgcgcccg ttaggtcaaa ttctgctgga aaatcaggtc atcactgaag
     6541 aacaactcga tacagcactg cgtaatcgcg tcgaaggtct acgcctgggc ggttcaatgc
     6601 tgatgcaggg gctgattagc gccgagcagc tggcacaggc gctggcagag caaaacggcg
     6661 tggcgtggga atccatcgat gcctggcaga tcccgtcctc gctgattgcc gaaatgccag
     6721 cctccgtagc gctgcattat gcagtactgc cgctgcgtct ggaaaatgat gagttaattg
     6781 tcggcagtga agatggtatt gacccggttt cgctggcggc cctgacgcgt aaagtcggac
     6841 gcaaagtgcg ttacgtcatt gttctgcggg gacaaattgt cacagggtta cgccactggt
     6901 atgcacgccg acgcggtcac gatccgcggg caatgttgta caatgcggtt cagcatcagt
     6961 ggctcacgga acagcagacc ggtgaaatct ggcggcaata tgtgccgcat cagttcctgt
     7021 tcgccgaaat actgaccacg ctcggtcata ttaatcgttc agcaattaac gtgttgttat
     7081 tgcgccatga acgcagttct ctgccgctcg gcaagttttt ggtcaccgaa ggcgttatca
     7141 gccaggaaac gttggatcgc gtcctgacaa ttcaacgcga attacaagtt tcgatgcaat
     7201 cactattact caaagcaggt ttaaacacag aacaggttgc gcaactggag tccgaaaatg
     7261 aaggagaata accttaatcg cgtcatcgga tggtctggtt tactgctgac gtctttattg
     7321 agtaccagcg cactcgcaga caatatcggc accagcgcag aagagctggg gctgagcgat
     7381 tatcgccatt ttgttattta tccccgtctc gataaggcgc tgaaggcaca gaaaaataac
     7441 gacgaagcaa ccgccatccg cgaatttgaa tatatacacc aacaggtgcc ggataatatt
     7501 ccgctgactt tataccttgc ggaagcctat cgtcattttg gtcatgatga ccgggcgcgg
     7561 ctgttgcttg aggatcaact gaaacgtcac ccaggagatg cccgacttga gcgcagtctg
     7621 gcggctattc cagttgaagt gaaaagcgtt acgactgttg aagaactgct tgcccagcaa
     7681 aaagcgggcg acgctgcgcc gaccctgcgt tgtcgcagtg aagtcgggca gaatgccctg
     7741 cggctggcac aattacctgt cgccagagcg caactgaacg atgcgacgtt tgctgcatcg
     7801 ccggaaggaa aaacgctgcg aaccgatctg ctgcaacggg caatctacct gaaacaatga
     7861 tcccaggcag atacgctata caatgaagca cgccagcaga acacattaag cgcggcagaa
     7921 cgccgtcagt ggtttgacgt gcttcttgcc gggcagctgg acgatcggat cctggcactg
     7981 caatcacagg ggatcttcac cgatcctcag tcatatatta cttacgcgac cgcgctggct
     8041 tatcgtggcg aaaaagcacg cctccagcat tatctcattg aaaataagcc gctgtttacc
     8101 acgggcgcac aagagaaaag ttggctctat ctgttatcta aatacagcgc caaccccgtt
     8161 caggcattgg cgaattatac ggtgcagttt gccgataacc gccagtatgt tgttggcgcg
     8221 acgctaccgg tgctgttaaa agaaggtcag tacgacgcag cgcaaaaact gctcgccacc
     8281 ctccccgcca atgaaatgct tgaggagcgt tatgctgtca gcgtggcgac ccgtaacaag
     8341 gctgaatctc tgcgtctggc acgattgctg tatcagcaag aaccgacaaa tcttccccgc
     8401 ctggatcaac tgacctggca actgatgcag aacgagcagt cacgcgaagc tgccgattta
     8461 ttgctgcaac gctatccttt ccagggcgat gcgcgtgtca gccagacttt aatggcgcga
     8521 ctggcgtctc tgctggaaag tcatccttac ctagcaacgc cggcgaaggt ggcgatttta
     8581 tcgaaaccct taccgctggc ggagcaacgt cagtggcaaa gtcagttgcc gggtattgca
     8641 gataattgcc cggcaatagt tcgcttgctg ggcgatatgt cgccttccta cgatgctgcc
     8701 gcctggaacc gtctggcaaa gtgttatcgg gacacgctac ccggtgtggc gttgtatgca
     8761 tggcttcagg ccgaacaacg acaaccgagc gcctggcaac atcgtgcggt agcctatcag
     8821 gcgtatcagg ttgaggacta cgccaccgca ctggcggcct ggcagaaaat cagtcttcac
     8881 gacatgagca atgatgatct gctcgccgcc gccaacaccg ctcaggcagc gggaaatggc
     8941 gcagctcgcg atcgctggct tcaacaggca gaacaacgtg agctgggaaa caatgctcaa
     9001 tactggtggc tgcatgcgcg acgttatatc ccagctcagc cagaactcgc actgaacgat
     9061 ctcacgcgct caatcaatat tgccccttct gccaactctt acgttgcgcg ggcgacaatt
     9121 tatcgccaac gtcataatgt tccggccgcg gtgagtgatt tgcgcgccgc gctggaactg
     9181 gaaccaaata atagcaacac ccaggcagcg cttggttacg ccttgtggga tagcggtgat
     9241 atcgcacagt cgcgggaaat gctcgaacag gcgcataaag ggcttccgga cgatccggca
     9301 ctgatccgac aactggccta cgtgaaccag cgtctggatg acatgcctgc gacgcagcac
     9361 tacgcccggc tcgtgattga tgacattgat aatcaggcat aaccccactg accccagagc
     9421 aaaatcagca acgcttcaat ttccgccgtt tgcatgagga ggtcggtcgc cgctggacgt
     9481 tcagtttcga ttcttccatc ggcttgcgtt ccggcgcaat gagtaccgct aacaataatg
     9541 tcggcggtgc agcgcctggg aaaagctatc gtagctacgg acaactggaa gccgagtacc
     9601 gcatcggacg caatatgctg ctggaaggcg acctgctctc agtttatagc cgcgtctttg
     9661 ccgataccgg agaaaacggg gtgatgatgc cggtgaaaaa tccgatgtcc ggcaccggtc
     9721 tgcgctggaa gccgctgcgc gatcagatct ttttcctcgc cgtcgaacag cagttgccgc
     9781 tgaacgggca aaatggcgca tccgatacca tgctgcgcgc cagcgcctca ttctttaatg
     9841 gcggcaaata cagcgacgaa tggcacccga acggttcagg ctggtttgcc caaaacctgt
     9901 acctcgatgc ggcgcaatat atccgccagg atattcaggc gtggacggca gattatcgcg
     9961 tcagctggca tcagaaggta gctaacggac agactattga gccttacgct cacgttcagg
    10021 acaacggcta tcgtgataaa ggcactcagg gcgcgcagct tggcggtgtc ggggtccgct
    10081 ggaatatttg gaccggcgag acgcactacg acgcctggcc gcacaaagtc agtctcggcg
    10141 tcgagtatca acataccttt aaggcgatta atcaacgtaa cggagagcgc aacaacgcgt
    10201 ttctcaccat tggagtgcac tggtaaatgc gtaagttcat tttcgtattg ctgacactgc
    10261 ttttggtcag ccctttttcc tttgcgatga aaggtattat ctggcaacca caaaaccgag
    10321 atagtcaggt taccgatacc cagtggcagg ggctgatgag tcagttacgt tttcaaggct
    10381 tcgataccct ggttttgcaa tggacccgtt acggcgatgc atttacccag ccagaacagc
    10441 gcacgttatt gtttaagcgt gccgcagctg cgcaacagac tggcctgaag ctgattgtcg
    10501 ggctgaacgc cgatccggaa ttttttatgc accagaaaca gtcgtctgca gcgctggaaa
    10561 gctatcttaa tcgcctgctg gctgccgatc tccagcaagc cagattatgg agcgccgcgc
    10621 ctggcataac gccggatggc tggtacatca gcgcggaaat tgacgacctg aactggcgca
    10681 gcgaagccgc ccgtcaacct ttgctaacat ggttaaacaa cgcgcagcgg ctgattagcg
    10741 atgtttcagc aaaaccggtt tatatcagta gttttttcgc cggaaacatg tcgcccgatg
    10801 gctatcgcca actgctggaa caagttaaag caaccggagt taatgtctgg gtacaggatg
    10861 gcagcggcgt ggataaactt accgctgaac agcgtgaacg ttatctacag gccagcgccg
    10921 attgccaaag ctccgcccca gcaagcggca ttgtttatga actgtttgtc gccggcaaag
    10981 gcaaaacctt tacagcgaaa ccgaaaccgg acgcagaaat tgcctcgctg ttagcgaaac
    11041 gttcctcttg cggtaaagac atgctctatt tctctctgcg ctatttgccc gtcgcgcacg
    11101 gcattctcga gtattaaatc ccctccaggt aagtcgggta cgacctggct tacctctttc
    11161 gctctcgtca gaattccatc aagattaatt gctaaaaagc atgctaaaga aagaagatcg
    11221 ttatcggcga acgggccgat cactcatgca ggaagcatag atcctttgag cggagtttct
    11281 gatatctgag gagtgcgaaa tgcaattgag cagcagtgaa ccttgcgtgg tgatcctgac
    11341 cgaaaaagag gtagaggtaa gcgtcaataa ccatgctacg tttacccttc cgaaaaacta
    11401 cctggccgcc ttcgcgtgca acaataacgt cattgaactc tcaacgttaa atcacgtatt
    11461 aatcacccac atcaaccgta acatcatcaa cgattatctg ttgtttttaa ataagaaatt
    11521 aacctgtgta aagccctggt cgcggctagc aaccccggtt atcgcttgtc atagccgtac
    11581 accggaagtg ttccggctag ccaccaacca cagcaagcag caatccagca aaccctgcga
    11641 agcggagttg acgcgcgcat tactttttac cgtattgtct aactttcttg agcaatcgcg
    11701 gtttattgcc ctactgatgt atatcttacg cagcagcgtc cgcgacagcg tctgccgcat
    11761 tattcaaagc gatattcagc attactggaa tctgcgaatt gtcgccagtt cgctatgttt
    11821 aagccccagc ctgctcaaaa agaaattaaa aaacgaaaat accagctata gccagattgt
    11881 cacagagtgt cgtatgcgtt acgccgtaca gatgttattg atggataaca aaaatatcac
    11941 tcaggtggcg caattatgtg gctatagcag cacgtcgtac tttatctctg tttttaaggc
    12001 gttttacagc ctgacaccgt tgaattatct cgccaaacag cgacaaaaag tgatgtggtg
    12061 aagggcaaag cggaaacgga taagacgggc ataaatgaag aagaaatggc gcgccctgca
    12121 ggattcgaac ctgcggccca cgacttagaa ggtcgttgct ctatccaact gagctaaggg
    12181 cgcgttgata ccgcaatgcg gtgtaatcgc gtgaattata cggtcaaccc ttgctgagtc
    12241 aatggctttt gatctggttg ctgaacaagt gaacgaccgc gtctgatttt ctgatttatt
    12301 tcgctatagc ggcaaacaaa cgcacaccgc tgcgcgtctg aatcaagaaa acccgtattt
    12361 tcatgtatca aagtacaatt taccgcccta acggaaaatt gtccgctcct atgagactgg
    12421 taactatgaa accaacgtcg gtgatcatta tggatactca tcctatcatc agaatgtcta
    12481 ttgaagttct gttgcaaaaa aacagtgaat tgcagattgt cctgaaaacg gatgattacc
    12541 gcataaccat cgattatctc cgaacccgtc ctgttgattt aatcattatg gatatagact
    12601 tgcccggaac agacggtttt accttcctg
//
LOCUS       DAJTUI010000102        12539 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTUI010000102 DAJTUI010000000
VERSION     DAJTUI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.102"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            118..1815
                     /gene="maeA"
                     /locus_tag="OO464_003254"
     CDS             118..1815
                     /gene="maeA"
                     /locus_tag="OO464_003254"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6228293.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1949..2959
                     /gene="adhP"
                     /locus_tag="OO464_003255"
     CDS             1949..2959
                     /gene="adhP"
                     /locus_tag="OO464_003255"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR6228294.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3105..3389
                     /locus_tag="OO464_003256"
     CDS             3105..3389
                     /locus_tag="OO464_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6228295.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3617..4270)
                     /gene="fdnI"
                     /locus_tag="OO464_003257"
     CDS             complement(3617..4270)
                     /gene="fdnI"
                     /locus_tag="OO464_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6228296.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4263..5147)
                     /gene="fdnH"
                     /locus_tag="OO464_003258"
     CDS             complement(4263..5147)
                     /gene="fdnH"
                     /locus_tag="OO464_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6228297.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5160..8207)
                     /gene="fdnG"
                     /locus_tag="OO464_003259"
     CDS             complement(5160..8207)
                     /gene="fdnG"
                     /locus_tag="OO464_003259"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7620..7622),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6228298.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8439..9320
                     /gene="yddG"
                     /locus_tag="OO464_003260"
     CDS             8439..9320
                     /gene="yddG"
                     /locus_tag="OO464_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6228299.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9575..10675
                     /gene="ompC"
                     /locus_tag="OO464_003261"
     CDS             9575..10675
                     /gene="ompC"
                     /locus_tag="OO464_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6228300.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10839..11324
                     /locus_tag="OO464_003262"
     CDS             10839..11324
                     /locus_tag="OO464_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6228301.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11434..12423
                     /locus_tag="OO464_003263"
     CDS             11434..12423
                     /locus_tag="OO464_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6228302.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3284 a         3107 c         2926 g         3222 t
ORIGIN      
        1 accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac gccctggcgg
       61 taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt gagtgacatg
      121 gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc tgtactgctg
      181 gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg taacttcaac
      241 ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga acgagcatgg
      301 atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg taacatccag
      361 gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga gatgatgcct
      421 gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat ctaccgccgt
      481 tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat tctgcaaaac
      541 gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat tctggggctt
      601 ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct ctataccgcc
      661 tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg aacgaacaac
      721 caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat cactgacgat
      781 gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg gccggacgtg
      841 ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa ccgctatcgc
      901 aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac agtcggcaca
      961 ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat cgtcttcctt
     1021 ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac ccagcgcgaa
     1081 ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt tggcctgctg
     1141 acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa gcgcgaaaac
     1201 ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt gcgcaatgta
     1261 aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga agagatcatc
     1321 cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa cccgacgtct
     1381 cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc gctggtcgcc
     1441 actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat cgcccagtgt
     1501 aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg cgcgtcacgt
     1561 atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc gccgctggtc
     1621 ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt ctcccgcgca
     1681 attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa aacgtctgct
     1741 gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg cgactaccgc
     1801 cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt ccctctccct
     1861 ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct cacctgctat
     1921 gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga aggatcatca
     1981 tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc tgctgaaaat
     2041 ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt ttggtgacaa
     2101 aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg gcccaggtgt
     2161 cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag gatgcggtca
     2221 ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa atgccggata
     2281 cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg cggtaaaagt
     2341 gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg tcaccaccta
     2401 caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct acggtcttgg
     2461 cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca aagtgatcgc
     2521 cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag atttagcgat
     2581 taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg gcgctcacgc
     2641 tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg ctgtccgtgc
     2701 aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg atatcccacg
     2761 tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc aggatctaac
     2821 cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc tgcgtccgtt
     2881 agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg gccgtatggt
     2941 gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt cgcagcatcg
     3001 cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg aacaataatg
     3061 atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa atggccaatc
     3121 atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc agcctgcgcg
     3181 agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg accggaaaag
     3241 cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt tcgccgcaaa
     3301 tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg gtggttgtgt
     3361 cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg ccatttgaaa
     3421 ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca cgttcacttc
     3481 aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc atccgggatg
     3541 aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc attttcagct
     3601 taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct gccttctcga
     3661 tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc ttcccttcga
     3721 tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc aggatggcgt
     3781 ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca acctgcatcg
     3841 ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc agcacgaaaa
     3901 tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta ccaacatccg
     3961 ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac ggaatatctt
     4021 tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt gcgacgaaaa
     4081 tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg aaggtttgcg
     4141 tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc aggaagaagc
     4201 aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca atcattttcg
     4261 acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc aataccgatg
     4321 tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag cggtttcaac
     4381 gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag accgtgatac
     4441 agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc gaccccttcc
     4501 gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac gcgctgttcc
     4561 gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg acaggttttc
     4621 acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca tttatatacc
     4681 cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat gcagtaacca
     4741 cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg aatgattgca
     4801 ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca gccgtcttta
     4861 cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat caccgtccag
     4921 gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc cacttcatca
     4981 cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat acaggtggaa
     5041 acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg cggcgtgatg
     5101 gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg ttcgccccct
     5161 tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga gtttgcatca
     5221 ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc ctcaaagccc
     5281 cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag cggtttcaga
     5341 cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac agtgacacga
     5401 tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac aaactgttcc
     5461 ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg ctcggtcaga
     5521 cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag cgcgtcttgt
     5581 tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt agtgcccagc
     5641 ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt gttgatggca
     5701 aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt acccggtgcg
     5761 gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca ctggatcagc
     5821 atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt gtagagtacg
     5881 cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc ggacgggtcg
     5941 gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt gtagatccag
     6001 caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag caactgacct
     6061 ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata gccgttgttc
     6121 tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca gctcatcttc
     6181 atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag atgatggtag
     6241 atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg acctttccag
     6301 tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc aaagcaggtc
     6361 gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt cgactcgccg
     6421 tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac catgtacttc
     6481 agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac cgggttaaag
     6541 ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata cttgatgacg
     6601 tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc tttctgcgcg
     6661 gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt gctccagtag
     6721 ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga ctgcaaatca
     6781 acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag cagacctaag
     6841 tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc accggccata
     6901 cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg cgcacccacg
     6961 gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg tgcgctggtg
     7021 gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca gatgttttca
     7081 acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac acagcgcgga
     7141 tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata gttccaggac
     7201 gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc ttcgaaagca
     7261 aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc ggcgttgatt
     7321 ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc cgtaccggaa
     7381 cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg atcgacaacg
     7441 atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc gacgggatgc
     7501 gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat atccacccag
     7561 tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt
     7621 cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa tttctgggtc
     7681 agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga aagccaacgg
     7741 tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc agctttcatc
     7801 agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt gtcagaacct
     7861 ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc cagcaaaccg
     7921 gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc ttcaatgtga
     7981 taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa tagcccgcaa
     8041 cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa tttgtagttt
     8101 cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt tgttccagcc
     8161 ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc tttcctcttt
     8221 tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt tttgcgcgaa
     8281 atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga cctacctcaa
     8341 tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct ataaaattca
     8401 gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa aaagcaacgc
     8461 tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt cgcggtgtca
     8521 gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc gggctgctgt
     8581 taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat ttactcgccg
     8641 ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg tatgcggcga
     8701 cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc ctgacaattc
     8761 tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca ggattattat
     8821 tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat tatgatgaaa
     8881 tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt ggtgcgttta
     8941 tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac ggaattaccg
     9001 tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg ccacaaccag
     9061 aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt accttaggat
     9121 ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata atggcggtag
     9181 gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc
     9241 tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg ctctgctggc
     9301 tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt aatcagataa
     9361 attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat atctcatttc
     9421 gacttcgatc acatatcgat aaataatatt attctatttt tctcattgtt tttaatcacc
     9481 attctatttt aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt cgtttagaaa
     9541 atacgtttaa cgataattaa aaggattctg ttaaatgaaa ttaaaaatag ttgcggtggt
     9601 tgtaactggt ttgttagctg cgaacgtagc acacgctgcc gaagtctata acaaggatgg
     9661 taataaactc gacctttatg gcaaggttac cgctctacgt tattttactg atgataagcg
     9721 tgacgatggt gataaaactt atgcccgtct cggctttaaa ggagaaacgc aaatcaatga
     9781 tcaaatgatt ggttttggtc actgggaata tgattttaaa ggctataacg atgaagccaa
     9841 cggctcgcgc ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta gtgaatttgg
     9901 ctctctggac tatggtcgta actacggtgt cggctacgac attggttcat ggaccgatat
     9961 gttgccagaa tttggtggcg atacctggag tcagaaagat gtcttcatga cataccgtac
    10021 caccggtcta gcaacctatc gcaactacga tttctttggc ttaattgaag ggctgaactt
    10081 tgccgcgcaa tatcaaggca aaaataaacg tactgacaac agtcatcttt atggtgctga
    10141 cgacacgcgt gccaacggtg acggtttcgg tatctcctca acttatgttt atgatggctt
    10201 tggtatcggt gcggtatata ccaaatccga tcggacaaat gcgcaggaaa gagccgcagc
    10261 taatcctctc aatgcctccg gtaagaatgc agaactatgg gccacaggta taaagtatga
    10321 tgccaacaac atctactttg cagctaatta cgatgaaaca ttaaacatga ccacctatgg
    10381 ggatggttat atctctaaca aagcacaaag ttttgaagtg gtggcgcaat atcaattcga
    10441 cttcggcttg cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc tgggccgcta
    10501 cgccgaccag gacatgattg agtatatcga cgttggtgcg acgtatttct tcaacaaaaa
    10561 tatgtcgacc tatgttgatt ataaaatcaa cctgattgat gaaagcgact ttacccgtgc
    10621 cgtagatatt cgcaccgata acatcgtcgc tacgggcatt acctatcagt tctaacgtcc
    10681 tctcctgacc agcccttctc tgggctggtt tttgatttaa gtgctgctgt atgactcctt
    10741 aagtaaaaat gagcaattct tgatatttct ggtcattcac ctcaattaat attattccat
    10801 atcctccggg gacatcttac tataatattt attgatgagt gattatgacc aatattaata
    10861 cagcttgtgt aaaaaataat gccagttatc aacttaataa cgcattacct aacaaagaaa
    10921 ccatctccag caatttttgt gaacgactag aacaatgggg taataaatcg gttaataatg
    10981 gtgaagaaag agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg aacatggcat
    11041 ctcttgattt gtcatatctt gatttgagtg aattaccgcc tatcccctcg acagttaata
    11101 cgttaaatct ggaaaacaat tgtctaactt gtcttgactt agctgacaat gccagtctcg
    11161 ttaatatcaa cctcagcgtt aataaaatta acacgataac ctttccaaaa gaaccaaaac
    11221 tggaaaacat ttatattaat cataacaatc tggaaagttt agatttaaaa aatcagcatt
    11281 cattggttaa tctggaagcg caaaataata gcctaaaaaa atgatatttc tgatagttat
    11341 aaactgaaat ttcttaatct tgattataac aagctagcat cgctggatgt ctcccggcaa
    11401 gaatctctga ttgagttaag tgcccatcac aatatgatca ctgaccttat attacacaat
    11461 catcccagga tgaaaacaat cactttaaac gacaaccata ttgcacattt aaacgccaaa
    11521 aacactacaa aactggaata tttaaactta agcaataaca atttactgcc aaccaatgac
    11581 attgatcaac taatatcatc aaaacatctt tggcatgtat tagttaacgg catcaacaat
    11641 gatccacttg cacaaatgca gtactggact gcagtaagaa atataattga tgacactaat
    11701 gaagtgacca ttgatttatc aggacttaat ttaaccactc aaccaccagg gctgcaaaac
    11761 ttcacctcta tcaatcttga taataaccaa ctcacacatt ttgatgcaac caactacgat
    11821 agactcgtaa agctaagtct gaatagtaat gctcttgagt caataaattt tcctcaaggc
    11881 agaaatgtaa gtattacaca tatatctatg aataataatg ctctcagaaa tattgatata
    11941 gataggcttt catcagttac ttattttagt gcggcacata atcaactaga gtttgtgcaa
    12001 ttagaatctt gcgaatggct gcaatacctg aatctcagcc ataatcaatt aactgatatt
    12061 gttgcaggta ataaaaatga actcttactg ctggatctat cccataataa actaacaagt
    12121 ttacacaatg tcttatttcc caacttgaat acgttactta ttaacaacaa tttgctttct
    12181 gaaattaaaa tattctatag caacttccgc aatgttcaga cattaaacgc tgctaacaac
    12241 cagttgaaat atataaatct tgatttcctg acttatcttc catctatcaa aagtttaaga
    12301 ctggacaata ataaaataac ccacattgat actaataata catccgatat tggaacttta
    12361 ttccccataa taaaacagag caaaaactta aattttttaa atgtttctgg gaagaacaat
    12421 tgacctacta tgcagctcat gttatttaat ttgttttccc cagcacttaa gcttaatact
    12481 ggcctggcaa ttctttcgcc tggtgcattt gaagttcact ctgacggaat agatgcgga
//
LOCUS       DAJTUI010000103        12525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTUI010000103 DAJTUI010000000
VERSION     DAJTUI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.103"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..1029
                     /locus_tag="OO464_003264"
     CDS             <1..1029
                     /locus_tag="OO464_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6228303.1"
                     /translation="NLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVW
                     FDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYD
                     AEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVI
                     GGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPH
                     DGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFN
                     TCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAP
                     IRPIARRT"
     gene            1030..3195
                     /locus_tag="OO464_003265"
     CDS             1030..3195
                     /locus_tag="OO464_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR6228304.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3209..4120
                     /locus_tag="OO464_003266"
     CDS             3209..4120
                     /locus_tag="OO464_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR6228305.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4120..5415
                     /locus_tag="OO464_003267"
     CDS             4120..5415
                     /locus_tag="OO464_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR6228306.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5460..5690
                     /locus_tag="OO464_003268"
     CDS             5460..5690
                     /locus_tag="OO464_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228307.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5668..6168
                     /locus_tag="OO464_003269"
     CDS             5668..6168
                     /locus_tag="OO464_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR6228308.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6168..7586
                     /locus_tag="OO464_003270"
     CDS             6168..7586
                     /locus_tag="OO464_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR6228309.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7586..8434
                     /locus_tag="OO464_003271"
     CDS             7586..8434
                     /locus_tag="OO464_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228310.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8434..8889
                     /locus_tag="OO464_003272"
     CDS             8434..8889
                     /locus_tag="OO464_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR6228311.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8892..9584
                     /locus_tag="OO464_003273"
     CDS             8892..9584
                     /locus_tag="OO464_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6228312.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9594..10925
                     /locus_tag="OO464_003274"
     CDS             9594..10925
                     /locus_tag="OO464_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6228313.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10926..>12525
                     /locus_tag="OO464_003275"
     CDS             10926..>12525
                     /locus_tag="OO464_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR6228314.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRN"
BASE COUNT         3364 a         3103 c         3303 g         2755 t
ORIGIN      
        1 aaccttgttg atcaccttct ggttaagcat cacacggctg atggcgttga agatggcatt
       61 tcaatctgct acttcaagcc atactcgcaa ggccgtgctc gctggcaggg tgacacaatc
      121 cacggcgtgt ggtttgacga agagccacca tacagcattt atggcgaagg tcttacccgt
      181 accaacaaat acgggcaatt ctcaattctg acgtttaccc cgctgatggg gatgtctgac
      241 gttgttacca agttcctgaa gaaccccagc aagtcgcaga aagtggtcaa catgaccatc
      301 tatgacgctg agcactatac cgacgagcag aaagagcaaa tcatcgcatc atatcccgag
      361 catgagagag aggcgcgtgc tcgcggtatt cctacgatgg gtagtggtcg aatcttccag
      421 ataccggaag agacgattaa gtgtcagccg ttcgagtgtc ctgatcactt ctacgtaatt
      481 ggcgggatgg atttcggatg ggatcaccca caggcgcaag ttcagctttg gtgggataag
      541 gaggcagaca caatctacgt ttcacgcgtg tggaaggcga aagaaaaaac agccgttcag
      601 gcgtggggag ccgttaaatc atgggcgcat aaagtgccaa ccgcatggcc tcatgatgga
      661 aaccagcacg agaagggcgg cggtgagcag ctcaaagggc agtatgcaga cgctggattt
      721 atgatgttgc aggagcatgc gacatggcct gatggcggta acgctgttga gccaggcatc
      781 actgaattgc gcgacatgat gctcgatggt cgcttcaaag tattcaatac ctgtgagcca
      841 ttctttgagg agttccgcct ctatcaccgt gatgaaaacg ggaaaatcgt caagcttaac
      901 gacgacgtgc tatccgccgt tcgctatgca tacatgatgc gccgcttcgc aaaaatgatg
      961 cgcgacatca aaaaaccaaa agagaaaaag ataccagccc caatcaggcc catcgcacgg
     1021 agaacttaaa tggccgacga aaacagactc aattccattc tgtgtaagtt tgacgcagac
     1081 tggatggcga gcgatgaagc cagaaccgag gcgacaaatg acctgtattt tagccgagtg
     1141 tcgcaatggg atgactggct atcaaactac actaccctgc aatatcgcgg acaattcgat
     1201 gttgttcgcc cggaggtcag gaaactggtc gcagagatgc gccggaaccc tgtcgacgtt
     1261 ctcttccgac ccaaagacgg cgctaatcct gatgcagccg atgtattgat ggggatgtat
     1321 cgtactgata tgcgccataa cacggcaaag attgccgtta acgttggcgt tcgtgagcag
     1381 atagagtcag gcgttggtgc atggcgtctg gtcacgcagt acgaagacaa cgatccaaca
     1441 agcaacaatc aggtaatccg acgcctgcca atccatgaag cctgctcaca cgtcatatgg
     1501 gacgccaaca gcaagcagat ggataagagc gacgctaagc actgcacggt gattaacgcc
     1561 ttgtcgcgca atggctggaa agagttcgca gaggattacg gtattgaccc ggataccctg
     1621 ccatctttcc agaatcctaa cgatacatgg ctattcccgt gggtgtcgaa tgatgtcgtc
     1681 tacgtcgctg agtattacga ggtagaagag gagaaggaga aggtcttcat ctaccgcgac
     1741 ccgctgacag gtgagccggt cagctattac cagcaggata tcaaagacgt catcgacgac
     1801 ctggctaatc gtggattcat taaggtagca gagcgcaagg tgaagcgtcg gcgtgtgtat
     1861 aagtcgatca tcacctgtac gcagatactg aaagaccgcg agaagatagc cggagagcat
     1921 attccaatcg ttccagtgta tggcgaatgg tcattcgctg gtgacaagga gtgctacgag
     1981 ggtgtggtaa ggctgacgaa agacggtcaa cgccttcgta acatgattat gtcgttcaac
     2041 gccgatattg ttgctcggtc accgaagaag aaaccgacct tcttccctga gcaaatcgaa
     2101 ggctacgaat acatgtacgg cggaaatgat gactatccgt actatctgca gaacaggact
     2161 gatgaaaacg gtaacgacct gccgattggt ccaatctcct acatggaaaa ccctgaagtg
     2221 ccgcaagcca acgcttacat gcttgaggct gccaccaacg cagtgaaaga agtggctagt
     2281 cttggcgtgg atgcgcaggc ggcaaatggt caggtcgctt ttgataccgt caatcaactg
     2341 aacatgcggg cagaccttga gacatacgtg tttcaggata acctggctac cgcaatgcga
     2401 cgtgatggcg agatttatgc ctcaatggtc aacgatattt atgacgttcc tcgtcatgta
     2461 acgctgacac ttgaagatgg aagcgagaaa gacgttcaac tctatgcgca agttgtcgat
     2521 taccagtccg gcaatgtggt cacactcaac gacattcgcg gtcgatatga gtgctataca
     2581 gacgttgggc catccttcca gagtatgaag gaacagaacc gcgcagagat tcaggagttg
     2641 ctcaccaagg ttccgcaagg tactccagag ttccagatgc tgatgctgca atacttcacg
     2701 ctgcttgacg gtaaaggcgt cgagatgatg cgagagtacg cgaacaagca actggtgatg
     2761 atggggctga agaaaccaga aacacctgaa gagatggaga tggtgcagca ggcacaacaa
     2821 cagccgcagc agccatcagc agagcaaatt caggcgcagg gtatccttct gcaaggtcag
     2881 gctgaattgc tcaaggcaga gaaccaacag gcgcagattc aggttgaagc tgccaaggtt
     2941 gaagcccaaa accaactcaa cgccgcgaag attgcagaaa tattcaacaa tatggacctc
     3001 gacaagcagg cagaactgcg tgagtacctc aggctcgtag gtcaattcca gcaacagcgc
     3061 agcaaagatg ctcgtgctaa cgctgagctg cttcttaaag atgcagacca gactcattca
     3121 caacgcatgg atttcgcgaa tcttatgcgt caagttcaaa tcccctccgg cggagtagcc
     3181 gagacacctc aataagagag agttaatcat ggaccaaacc accgacattc aggcttctga
     3241 agaattaacc ctgcccggca atcatgcagc ggcatctgct gatggcttag ttgtcgataa
     3301 tgccaacgac agcgcaggtc aggaagaagg ctttgagatt gtccggaaag acgatgagaa
     3361 accaaaacaa gacccggcaa ctaatgctga atttgcccgt cgccgcatcg aacgcaaacg
     3421 ccagcgtgag cttgagcagc agatggaagc ggttaagcgt ggagagttgc cggagcacct
     3481 gcgggtgaac cctgagttac caaaacaacc agaccctaac gattatcttt ccgaagatgc
     3541 actggctaag tacgactatg accagagccg cgcactggct gccttccagc aggcaaacag
     3601 tgaatggcag atcaaggcta tggacgcacg aagccaggct gtcgccgagc agggtcgcaa
     3661 aactcaggag ttcacccagc aatcagcgca atacgtcgag gcggcccgta agcactacga
     3721 ggcagcggaa aagctcaata tccctgacta tcaggagaaa gaggatgcat tcatgcaact
     3781 ggtgccgcca gcagtcggtg ccgacatcat gcgcctcttc ccggagaaat ccgccgctct
     3841 catgtatcac cttggtgcta atcctgagaa aacacgccag ttgctggcga tggacgggca
     3901 atccgcgctg attgaactca ctcgactgtc agaacgttta actctcaagc ctcgagccaa
     3961 gcctgtttca gaagccccgt tacctgatga acccattcag ggacatgctg ttgctgcaaa
     4021 tatctctgcg attgaaaagc agatggaggc ggcagcaaac aaaggggatg tagagacgta
     4081 ccgcaagctc aaggcgcaac tgaataaagg aattcgataa tggcattaaa tgaaggtcaa
     4141 ctggtcacgt atgctctgga tgaaatcatc ggaaccgtcc agaacctgac gccaatggcg
     4201 tcaaaagtga caaaataccc ccctccggca gaatccatgc agcgttcaag caacaccgtg
     4261 tggatgcctg ttgagcagga agcgccaacc cagactggct gggatttaac tggcaacgca
     4321 accgggattc tggaactctc cgtgaagtgc aacatgggcg atccggataa cgatttcttc
     4381 gagcttcgtg cagatgacct gcgtgatgag cgttcttacc gtcgccgcat ccaggcatcc
     4441 gccaaaaaac tggcgaataa cattgagtca gcgattgcca aacaggcaac tgaaatgggc
     4501 tcgcttgttg ttcacgatac ccgcgcaatt ggtccatcga ctggcctgcc tggctgggat
     4561 tttgtgtctg atgcagagcg actgatgttc tcccgtgagc taaaccgcga catgggcatc
     4621 agttacttcc tgaaccctga cgattaccgc aaagcaggcc gcaacctggt agatggtgac
     4681 atcttcgggc gcgttcctga agaagcatat cgaaacggta ctattcagcg tcagattgct
     4741 ggctttgatg aaattcttcg ctcaccgaaa cttccggcag ttaccaagtc aaccgcgact
     4801 ggtgtaactg tttctggtgc gcagaagttt aagccgcagg catacaccct tgataccgat
     4861 ggtaacaaag agaacgtcga caaccgtgtt gcaacggtga ccgtatcctc caccaccgga
     4921 tttaagcgcg gcgacaaaat cagcttcatt ggtgtgaagt tcctgtctca gatggcgaag
     4981 aatgtgctga ctgatgacgc gactttctca atcacccgtg tgatcgatgg tactcacatc
     5041 gaaatcacgc cgaagccgat tgcgctggat gacgcgtcac tgacaaaaga agagaaggct
     5101 tacgctaacg taaacacctc tcttgctgat accactccgg taaacgttct gaacgtggca
     5161 acaaccaccg ctaacgtgtt ctgggctgat gactcaatcc gcctgctgtc tcagccgatc
     5221 ccggtaaccc atgaactgtt tgctggcatg aaaacgtctt ccttcagcat tccaggtatt
     5281 ggtgttaacg gcatcttcgc aacgcagggt gatatcaaca ctctgtctgg taagtgccgt
     5341 attgctgtgt ggtattcagc atgtgctgta cgaccagagg caattggtgt tggtctgcct
     5401 aaccagaccg cgtgataacc agagggagct tcggctcccg tttttatctg gagacaagca
     5461 tgacacacat gatctttcgt catggcgaca tgaagaagtg gaaaggcgtt ggctacgact
     5521 ttgaaatcgt gaaagccgaa gagcttcagg aatatctgga tgctggctgg ttttcacatc
     5581 ctgatgacct tttgaaggat gttgcagagc cagagccaga agaaaaacag cgtaaaaagc
     5641 ctggtcgaaa acctaaggcg gcagcagatg aacctgacaa cgaagggtga tttagttctt
     5701 gcggcattac gtaagctcgg tgttgcatca aatgccacgt taaccgatgt cgaaccgcag
     5761 tctatggaag acggcgtcaa cgaccttgaa atgatgatgg ctgaatggct tggcggtgat
     5821 gcgtcacctg gtatcaacgt tggctacatt tttgctgatg cagatgttgc tccggatccg
     5881 ggcgatgagc acggtttatc aaataacgct atcaatgccg tcattttcaa ccttgcctgc
     5941 cgaattgctc cagattatgc gctggaagcg tctgcaaaac ttataaccac tgccagatac
     6001 gggaaagagc gactcgtcaa actgtctgca atggacaggg caaaagcagc taaatgtaag
     6061 tccggttatc caaaccgtat gcctgttggt agtggtaacc agttggcgaa gtggaatggt
     6121 tggaattact tccaccgaaa ggaaccttgc gataacggga gcgaataatg cctattcagc
     6181 aacttccgct tatgaaaggt gtcggcaaag acttccgaaa cgccgactat atcgactatc
     6241 tgccagtgaa tatgctggct acacccaaag aaatcctgaa cagcagcgga tatcttcgct
     6301 cattcccggg cattgccaaa cgttctgacg tgaacggtgt atctcgcggc gtcgagtaca
     6361 acatggcgca gaatgctgtt tatcgcgtgt gtggcggcaa gctgtacaaa ggagaaagtg
     6421 aagtcggtga tgttgccgga agtggtcgtg tatcaatggc gcatggtcgg acatcacagg
     6481 cggtaggcgt taatggtcaa ctggtcgagt atcgctatga tggcacggtc aaaaccgtct
     6541 caaactggcc tacagacagt ggattcactc agtatgagtt aggttcagtt cgcgacatta
     6601 cgcgcttacg tgggcgttat gcgtggtcaa aagacggaac tgattcatgg tttatcactg
     6661 accttgaaga cgaatctcat cctgaccgtt acagcgcaca atatcgtgcc gagtctcagc
     6721 ctgacggcat catcggtatc gggacatggc gagacttcat cgtctgcttt ggttcatcga
     6781 ctattgaata tttctccctg actggcgcaa ccaccgttgg tgccgctttg tatgttgcac
     6841 aaccatcgct gatggtgcag aaaggcattg ccggaactta ctgcaaaacg ccattcgctg
     6901 attcgtatgc gtttatcagc aatccggcaa cgggtgcgcc gtctgtgtat atcatcgact
     6961 ccggtcaggt gtcaccaatt gccagcgcga gcattgagaa aatcctccgc tcctacactg
     7021 ctgatgaact ggctgatggt gtgatggaat cgctgcgatt tgatgctcat gagttgctga
     7081 ttatccacct tccgcgtcac gtcctcgtgt acgacgcatc ttcaatcgcc aatggcccac
     7141 aatggtgtgt gctgaaaaca ggtctgtatg acgatgtgta ccgcgctatc gacttcgttt
     7201 acgaaggcaa tcagataacg tgcggcgata agctggaatc tgttaccggg aaactgcaat
     7261 tcgatatcag cagccagtac gacaagcaac aggaacacct gctgtttact ccactcttca
     7321 aagcggataa cgccagagtg ttcgaccttg aggttgaatc gtcaactggc gttgcgcagt
     7381 acgccgaccg cctttttctc tctgcaacca ctgactgcat caattacggg cgtgagcaga
     7441 tgattgagca gaatgaaccg ttcgtttacg acaagcgtgt tttgtggaag cgagtagggc
     7501 gcatcaggaa aaatgtcggc ttcaaattgc gcgttatcac gaagtcacct gtcactctgt
     7561 ctggcgctca gataaggatt gagtaatggc tgattcgaat ctcaatgtgc cggtaatcat
     7621 ccaagctacg cggctcgata catcagtcct tccacgcaat atcttctcgc agtcgtatct
     7681 gctttacgtt atcgcacagg gcactgatgt tggtaacgtg gctaacaaag ccaacgaggc
     7741 cggacagggc gcttatgatg cacaggtcag gaacgatgag caggatgtga ttctcgctga
     7801 ccatgagcag cgaatttctg ctgcggaagc aacgcttgtt aatcatgagg agcgaatcag
     7861 acaggcagaa tcaactcttc aggaccatga aacacgaata gctcagaatg aaagcgatat
     7921 ttcgtcgctt gacacaagag ttcagtcgct ggagtcgcag gtttcagacc atgaaacgcg
     7981 catcgatgct ctggagtatg caaccacacg caagaagtca gaggttgttt actctggtgt
     8041 atctgtaacc atcccgacag cgccgaccaa ccttgttagc ctgctgaaaa cgctcacacc
     8101 gtcatccggc tcgttggcac cattcttcga caccgttaac aacaagatgg ttgtgttcaa
     8161 cgagaacaaa accctgctct tcaagttgtc gattgtcggc acgtggccca gcggaaccgc
     8221 caacaggtca atgcagctaa ccttttccgg ctctgttcct gacacactgg tcagcagtcg
     8281 taatgcggcg acaacaaccg acaacatcct gttagctacg ttcttcagcg tggataaaga
     8341 cggctttctt gccacaaatg gcagtacgtt aaccattcag tcgaatggtg cggcgtttac
     8401 tgccacaacc atcaagataa tcgcggagca gtgatgatta cattcaaacc aacgcgaaac
     8461 atcgacctga tagaagcagt cggaaatcac cctgacatta tcgccgggag caacaacggt
     8521 gatggatacg actacaaacc tgaatgccgt tactttgagg tgaacgtgca cggtcagttt
     8581 ggcggcattg tttactatca ggagattcag ccgctgacat tagattgcca cgccatgtac
     8641 ctgccagaga ttcgcggctt cagcaaggaa attgggctgg cgttctggcg atacattctg
     8701 actaacacca ccgttcagtg cgtaacatcg ttcgctgcac gcaaattccg acacggtcag
     8761 atgtactgcg caatgattgg ccttaagcgt gtaggaacca tcaagaaata cttcaaaggc
     8821 gtggatgacg tgacttttta cagcgccaca cgcgaagaac taatcgactt cctgaatcac
     8881 gggagatagc catgttatat gcatttaagc tgggcagaaa actgcgcggc gaggaacctt
     8941 attgccctga aaaaggcggg aaaggtggca gttctgataa aagcgcaaag tatgcagcag
     9001 aagcccagaa gtatgctgca gacctgcaaa atcagcagtg gcagacgatc atgaaaaacc
     9061 ttgctccgtt cacgcctctt gcggagcagt atgttaacca gcttcagaac ctttccagtt
     9121 tagaaggtca ggggcaggca cttaatcagt attacaactc taagcagtat aaagaccttg
     9181 cagggcaggc gcgttaccag agtcttgctg ctgcggaggc gacgggtgga cttggttcga
     9241 cagccacaag caatcaactg gctacgatcg cgccgacact cggtcagtct tggttatcaa
     9301 atcagatgag caattacaac aatctggcaa acgttgggct tggtgcgctg caaggtcagg
     9361 caaacgccgg gcagacgtac gccaacaaca tgagcagcat tgcacagcaa agcgcagctc
     9421 ttgccgctgc taatgccaat aaaccatcaa gtcttcagac tgcaattagc ggtggcacgt
     9481 ctggtgcgat tgccggtgca ggtcttgcca gccttttggg aacatcaacg ccttggggcg
     9541 ctggtattgg tgctggtatc ggattgcttg gctcgttgtt ttaaggggga atcatggcta
     9601 cttggcaagg aacaaacggc ggattgttgg ctggtatcgg cggcgtcaac tcaaacgctc
     9661 cgagcgtaaa tgacatcggc aatacgcttc agcttatcag gcagaacaat gatattgagc
     9721 gttcaggcgc taacaatgtt gggctgactg ctttgcaagg tctttcaggt attgcgggtg
     9781 tttttcagca ggaaaagcag gctcagcggc agaaagaatt tcagcaggcg tacgctaatg
     9841 cttatgcgtc tggtgatcgc ggtgctttgc gtcagttggc tactcaatat ccagaccaga
     9901 ttgaatccgt tcgtaaaggc atgggattca ttgatgaaga gcagcgtaat tctatcggca
     9961 ccttagcggc tggcgcacgc cttgcgtcat cgtctccaga agcaatgcaa tcatggctgc
    10021 aaaacaacgc cggtgagtta gctcgtgttg gcgttaatcc tcatgacgtc gctcagatgt
    10081 accaacagaa cccgcggcag ttcggcgaat ttgtcgatca cctggggatg aacagtctcg
    10141 ggcccgaaaa atactttgac ctacaggata aaatgcaggg tcgccaggtt accatgcgcg
    10201 gtcaggatct ggattcgcaa accgccgctc ggaatcaggc aatcacaatg cgcggacaag
    10261 atatccaggc gaatttaggt cagcagcgca ttaatctgga cgcagaaaca aaccgcatta
    10321 acaacgaaaa taagcgcctt gaccggatgc tatcagcaga aactaacgac ctgaagcgcc
    10381 aggaaataca gagccgcata gcagccaaca accagcagtt gcagcagaag cagcaagcgc
    10441 taaatgatgg ctacaaagac ggcatcaaca ccctcacaac cagcatgttc actctgaacg
    10501 atatcgttag ttctccttca cttaagagca ttacaggctt acgtggagta atccccaacg
    10561 ttccaggctc acaggctgca gacactcagg cacgacttga tacctttaaa tcacaagcat
    10621 acctgacagc ggttcaggcc atgcgaggca tgggcgcact ttctgatgcc gagggcaaaa
    10681 agctcgacca ggctgttggt tcgctgcaga actcgcagag cgaggagtcc tttcgtcgca
    10741 acgctggcgt catcctgaac acgctcaacc agaagcgtaa tgaggcggtt ggtaagtacg
    10801 ttcagcaaaa cggtatcaag cgagtggaag cgcctcaggc ttctatagat tacctgaagc
    10861 agcaccccga gctgtcaatc gacttcatta atcgctacgg atatcttcca tctttggggc
    10921 agtaaatggc taattaccgt gatttgttag agcaggctgg cgcacgttac ggtgtgccag
    10981 aagggttgat gactgcactg ggtgccaagg agtcttctta caaccctgcc gcagtaagct
    11041 ccgccggggc tgtaggattg actcaggtca tgcctgggac atggcgtgat atgggttata
    11101 ccgatgagca aatgcaaaac cccgaatatc aggctgacgc tggcgcgcgc tatctggcaa
    11161 agatgtacca gcagtttggt aactggcgtg actctcttca ggcttatcac gacggtcccg
    11221 gcaacgttat gaaggcaaag cgtggtgaat atacgccagg acctgaaggc cgcggttacg
    11281 ttgatgatcg ctttgctcaa tgggctggtg acccggtgac agactcaaca gtcgaacagc
    11341 gcgccacctc tgcaaaggta catcctcagc aagaccctaa caacccgttt gcacaactgg
    11401 aagtacagtc atccgaacaa gtatcggcat caggtgtgca gtcagaccca aataatccat
    11461 ttgctcagat tgagcagcag gcagccagtc agcagccacc tcaacccgta agttctgtcg
    11521 caccgaaacc tgttcagcaa caaacagtta atcaggccaa taatgcacca acacgggaag
    11581 aaccatcatt gatgcaacaa gctggcgatt ggctcactgg tggtcaaagt gcagggcaaa
    11641 ttgcagaaca ggctggtcgt ggtctggtaa acataccatt tgacgtattg cagggtggcg
    11701 caagtctgat taatgcaatc agtcaggggc ttggggggcc aaaagtattg gatgatgttt
    11761 atcgtccagt agacagaccg acagacccct acgcgcaagc tggagagtca ataggcggtt
    11821 atcttgttcc tggagcaggt gtagctggaa acatggtcat tggttctctc gctgacgcgg
    11881 cgaatcaacg gggtgatttt gccgaaaatg ccgctattaa tgccggactt agcattgcta
    11941 cgcatggcct gataaatggc gttacccgtg gtgttcgtgg tgcatcaaat ataattagtg
    12001 gcaataaaac atctgcacag agagcgacca ctgcgccaac agaaacatca ccattctccg
    12061 gtgatgccgc tgcagcaaca aatcctgcgg ttcatgccgc agaggcaaga gtagcacaag
    12121 gtgtaccaat gacgcctgcg acgaagaacc cagaggaagt cgttcgcaca gtagcagcac
    12181 aaaaaaggcc aaatctcgct tcatcgcttg atgaactaga tatcaatcct caggctgaag
    12241 ttctggagtc tgctgaaagg cttaatgttg attcattact cccttcacac ttttccggga
    12301 acgagcaata caaggcagtt gagcaagcaa tcaagtcccg tgcgggttct gctctacagg
    12361 tgcaggaaaa tgaagcaatc aggcagctag cacagggcgc gggtgagata attgatcgcg
    12421 tttccggtgc aaaagatgct cttggtatga gcgacaagtt tattgatacg gtcaatggaa
    12481 gaatgtctgc gctgatgaaa cgaagcgacc agctttatcg caatg
//
LOCUS       DAJTUI010000104        12398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTUI010000104 DAJTUI010000000
VERSION     DAJTUI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.104"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            508..591
                     /locus_tag="OO464_003276"
     CDS             508..591
                     /locus_tag="OO464_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6228315.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(645..1997)
                     /gene="gorA"
                     /locus_tag="OO464_003277"
     CDS             complement(645..1997)
                     /gene="gorA"
                     /locus_tag="OO464_003277"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6228316.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2069..2911)
                     /gene="rlmJ"
                     /locus_tag="OO464_003278"
     CDS             complement(2069..2911)
                     /gene="rlmJ"
                     /locus_tag="OO464_003278"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6228317.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3114..5156
                     /gene="prlC"
                     /locus_tag="OO464_003279"
     CDS             3114..5156
                     /gene="prlC"
                     /locus_tag="OO464_003279"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6228318.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5164..5916
                     /gene="rsmJ"
                     /locus_tag="OO464_003280"
     CDS             5164..5916
                     /gene="rsmJ"
                     /locus_tag="OO464_003280"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6228319.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(5965..7434)
                     /gene="dtpB"
                     /locus_tag="OO464_003281"
                     /pseudo
     CDS             complement(5965..7434)
                     /gene="dtpB"
                     /locus_tag="OO464_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(7752..8186)
                     /gene="uspA"
                     /locus_tag="OO464_003282"
     CDS             complement(7752..8186)
                     /gene="uspA"
                     /locus_tag="OO464_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6228320.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8580..8915
                     /gene="uspB"
                     /locus_tag="OO464_003283"
     CDS             8580..8915
                     /gene="uspB"
                     /locus_tag="OO464_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6228321.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(8986..10485)
                     /gene="pitA"
                     /locus_tag="OO464_003284"
     CDS             complement(8986..10485)
                     /gene="pitA"
                     /locus_tag="OO464_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6228322.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10717..11916
                     /locus_tag="OO464_003285"
     CDS             10717..11916
                     /locus_tag="OO464_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6228323.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3042 a         3129 c         3294 g         2933 t
ORIGIN      
        1 gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt cagctggaga
       61 agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat cgggtcgctg
      121 ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc ggttttttta
      181 ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc taacgcatca
      241 atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc taattacact
      301 gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat aaacgctttc
      361 aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg gagtgagatt
      421 gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa gcggatgcat
      481 tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt tcttggggcg
      541 ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg atagcggaaa
      601 cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg catggtcacg
      661 aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt tttggttgcg
      721 cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa gccaatgccg
      781 tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg gcacggctgg
      841 cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac tttcacctga
      901 tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc aatcggcgga
      961 tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg cttgttatta
     1021 aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag ttccactgca
     1081 cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata tttatcgacg
     1141 acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat gttgtcattg
     1201 gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg accatcttcc
     1261 agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat ggcgttggta
     1321 tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat catcgggtcg
     1381 aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc gaggccgtta
     1441 atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac gcgctctggc
     1501 aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc tggaatatcc
     1561 gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat ggtttcgccg
     1621 ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac atcaacgtta
     1681 tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc ggtacggctg
     1741 gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata atcagggccg
     1801 tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt cggcacgcag
     1861 ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca tttctggccg
     1921 tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat ggcgatgtaa
     1981 tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt gtagcgcgag
     2041 acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt acggtggcgt
     2101 gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta ttcatctgct
     2161 gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg ccacggcgat
     2221 cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg gtcgcttcca
     2281 ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac aatgcgtaag
     2341 tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc tgatagtcgg
     2401 ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc ggcggcagtt
     2461 tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg tctttctgaa
     2521 atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc aggctgtctt
     2581 gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc aactggccgc
     2641 tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc aaatcgtcct
     2701 gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca tgttcgctac
     2761 ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt ttatctttct
     2821 ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg gcgtggttgc
     2881 cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta agtaaaaatt
     2941 ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag aggccgccgc
     3001 cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc tacacacatt
     3061 atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg ctaatgacga
     3121 atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg gaacatgtcg
     3181 ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc gtagtagcgc
     3241 aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg gacgatgttt
     3301 tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc ccggaactgc
     3361 gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg gtagggcaac
     3421 atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac gccacgctga
     3481 acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc tctggcatag
     3541 gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt tctgaactgg
     3601 gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa ctcgttaccg
     3661 acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa gcccaggccg
     3721 aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac ctgccggtaa
     3781 tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac agcacccgcg
     3841 cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg gaagagatcc
     3901 tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc tttaaatccc
     3961 ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc gatctggcaa
     4021 aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc gctaaagccg
     4081 aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa aaacagaaac
     4141 agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa aacaaagcgg
     4201 ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa gagcgtaaag
     4261 atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa aataacgaac
     4321 tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc ggggcgtgga
     4381 tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa ccggtcgcgt
     4441 atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc actcacgacg
     4501 aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg acccgcatcg
     4561 aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa ctgccgagtc
     4621 agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct ggtcactatg
     4681 aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg aagaactacc
     4741 aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc cgccttcatg
     4801 ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa atcaagaaac
     4861 tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc agccatattt
     4921 tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta ctggcggcag
     4981 atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg cagtcgttcc
     5041 tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc aaacgcttcc
     5101 gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag ggctgatcat
     5161 tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt atctgttctg
     5221 gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt gttaacgccg
     5281 gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt tgttgatttt
     5341 gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga ggcggtggcg
     5401 aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac cgcagggctg
     5461 gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct ggagcgtaat
     5521 ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga tgcggaaatc
     5581 ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac ggcgctaacc
     5641 gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca taagcagaaa
     5701 agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc ggatcttgat
     5761 gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt ggtaaagcgc
     5821 ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt gactaaaggg
     5881 catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt agcgataacg
     5941 ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca tgcgtttcag
     6001 ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac caatcttacc
     6061 aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt ccggcaccgc
     6121 ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca ggaaccacat
     6181 cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg ccaggccaag
     6241 ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga taaaccatgg
     6301 cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg tcaaaaagcc
     6361 cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt ctttgccttt
     6421 gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca cccagaacgg
     6481 gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat gatgcacgtt
     6541 gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt aaaacaccac
     6601 cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt tatccagctt
     6661 gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca gcaccagatt
     6721 ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca cgctgccaag
     6781 taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag aaccaatgtc
     6841 tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc ccgccccgca
     6901 caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca gcgataacgc
     6961 tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat caagccgcgg
     7021 atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa acaggccgtt
     7081 accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta gcgacatgcc
     7141 ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc gtttggtccc
     7201 caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca gtgcagcaaa
     7261 agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa cgaagaaaac
     7321 cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga caaaaaagat
     7381 catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat taatatttat
     7441 taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg cgaaaaagtt
     7501 tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac tattacaaca
     7561 gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa ttttgtattg
     7621 gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta cgctgcatca
     7681 ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag gaacacgtcg
     7741 tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc aacgtgaacg
     7801 gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg gtggtgacca
     7861 caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg gcctaggtcg
     7921 ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag ctcggtcagc
     7981 gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac atcaataagc
     8041 ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt cgcattgtag
     8101 gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga gagatcgact
     8161 gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg tcgatgactg
     8221 gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac gtgcgcgggg
     8281 ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg acccggatga
     8341 tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga atattatatt
     8401 gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc tatagagccg
     8461 ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac tgattggcag
     8521 cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg ggaggaggta
     8581 tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt aacatggcgc
     8641 gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat ccattgctct
     8701 atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac aaacaggtgc
     8761 gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat gagtttattc
     8821 gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt ggtctggtgg
     8881 tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa taacgtataa
     8941 aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca ggaactgcaa
     9001 ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa acacccaggc
     9061 catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca ccatcgtccc
     9121 cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat aactcgccag
     9181 gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg tcatgccttt
     9241 cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca tcgtaccgat
     9301 acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga tggtgctaag
     9361 catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac caggcatctt
     9421 caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct ggctacgttg
     9481 atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt tcaggcggtt
     9541 gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg ttgcgccagc
     9601 ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg caggatgctg
     9661 ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt cgtagccagt
     9721 ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca acataaccag
     9781 accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga tagcggaaag
     9841 gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct tttcacgctc
     9901 cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc gacgcagcaa
     9961 gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca gagaaccgaa
    10021 aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg tcatcaacgc
    10081 attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg atgcaggtaa
    10141 accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag agaacaccat
    10201 ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca tatgcacaat
    10261 ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca ccgccgccat
    10321 aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca cggcgttggc
    10381 tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg caagcaataa
    10441 taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac gttacgccat
    10501 tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca aagtgaaata
    10561 tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt atcttatata
    10621 attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg aggaatcttt
    10681 acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga tgccattatt
    10741 ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt aggacgccgg
    10801 gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc tggcggtggg
    10861 cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca gaatccgcat
    10921 ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct ggtcaataaa
    10981 cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga ctccgcgcag
    11041 cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt cagattgcgt
    11101 agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct gaacggcatg
    11161 actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc ggggctgggc
    11221 gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct gccgacccgc
    11281 gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca ggtgctggcg
    11341 ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg tgagaactta
    11401 ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag ctactggcaa
    11461 ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac cttcctgaat
    11521 gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt tcatctaccg
    11581 aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc gctaaaacag
    11641 ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg cgtacaaccc
    11701 aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga caccaacgaa
    11761 ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat cggtgaagtg
    11821 atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc gagtgcatgg
    11881 gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc cgtcagccgt
    11941 ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt ctggtgttaa
    12001 ctcgctgttt tgttagcgaa ttaacgctat tgccgttgtg gttattttat agtgtattta
    12061 ttctcagaga atatatatta ctgaaataaa aaacacaaag aataaccaaa ggaaattatt
    12121 tgaaacgtaa aaggtatata tttttaatct gtaaaattta aaatcacctc ctctggattg
    12181 aatcaacttg ctctatttaa gacgattgtt tttacatttt caccacctgt atcaggtggt
    12241 gtttttttat tggcggcaat gcctttatct tatttttatt acgttgtaaa tatggctatt
    12301 cctaaatgaa atttgatttg ctcagtttat ttgtgatgtc aggggagtgc ttgccatgag
    12361 agtcgtgata ttattattca tcatttttag gtaatgct
//
LOCUS       DAJTUI010000105        12222 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTUI010000105 DAJTUI010000000
VERSION     DAJTUI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.105"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..492)
                     /locus_tag="OO464_003286"
     CDS             complement(<1..492)
                     /locus_tag="OO464_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6228324.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(523..1215)
                     /gene="fimC"
                     /locus_tag="OO464_003287"
     CDS             complement(523..1215)
                     /gene="fimC"
                     /locus_tag="OO464_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6228325.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="OO464_003288"
     CDS             complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="OO464_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6228326.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2458..3324
                     /gene="folD"
                     /locus_tag="OO464_003289"
     CDS             2458..3324
                     /gene="folD"
                     /locus_tag="OO464_003289"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6228327.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3326..3538
                     /gene="ybcJ"
                     /locus_tag="OO464_003290"
     CDS             3326..3538
                     /gene="ybcJ"
                     /locus_tag="OO464_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6228328.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3646..4155
                     /locus_tag="OO464_003291"
     CDS             3646..4155
                     /locus_tag="OO464_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6228329.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="OO464_003292"
     CDS             complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="OO464_003292"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6228330.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5750..6244
                     /gene="ppiB"
                     /locus_tag="OO464_003293"
     CDS             5750..6244
                     /gene="ppiB"
                     /locus_tag="OO464_003293"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6228331.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6247..6969
                     /gene="lpxH"
                     /locus_tag="OO464_003294"
     CDS             6247..6969
                     /gene="lpxH"
                     /locus_tag="OO464_003294"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6228332.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7087..7596
                     /gene="purE"
                     /locus_tag="OO464_003295"
     CDS             7087..7596
                     /gene="purE"
                     /locus_tag="OO464_003295"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6228333.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7593..8660
                     /gene="purK"
                     /locus_tag="OO464_003296"
     CDS             7593..8660
                     /gene="purK"
                     /locus_tag="OO464_003296"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6228334.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8797..9690)
                     /locus_tag="OO464_003297"
     CDS             complement(8797..9690)
                     /locus_tag="OO464_003297"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6228335.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9687..10334)
                     /locus_tag="OO464_003298"
                     /pseudo
     CDS             complement(9687..10334)
                     /locus_tag="OO464_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10337..11569
                     /gene="allC"
                     /locus_tag="OO464_003299"
     CDS             10337..11569
                     /gene="allC"
                     /locus_tag="OO464_003299"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR6228336.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11580..>12222
                     /gene="allE"
                     /locus_tag="OO464_003300"
     CDS             11580..>12222
                     /gene="allE"
                     /locus_tag="OO464_003300"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR6228337.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYN"
BASE COUNT         2967 a         3160 c         3117 g         2978 t
ORIGIN      
        1 agcgctactc aacaacgcta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc
       61 aaagttaatt gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg
      121 ctgggctaac tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt
      181 aaaacagggc attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac
      241 gatatcctgt gaaccaatga actcatcatt acgccagaga tccacccgat acacgcccgc
      301 aggttgatga ttacctctct caaagcgcga taaatcagca acggatgtgc cattctctaa
      361 taaaaatgcc gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag
      421 agatgaataa caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt
      481 gggtattttc ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc
      541 tctgaccggg gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac
      601 tcctgagggt aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt
      661 ttggctgccc acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt
      721 gatatagtta ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg
      781 taattgaatt ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt
      841 cctgccgttc aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag
      901 agactcacga tctgccgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct
      961 tttgggttca ctaacaaata gtggcggtgt aatgatgaat gacttttctt ttacaccgct
     1021 gctgttttca atccacgaat tgaccagaaa gagctcattg gtatggctat ttttaatcca
     1081 taccgcagtc tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc
     1141 tccggcagca tgggcgctgg ccgaaatgat taaataaaat atcatgagca tcaataactt
     1201 tattttagtc atcatagtta atacgccttg tgtattcctg acggcaaaaa taaggagtat
     1261 aaagaaatga ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg
     1321 catgatacaa agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg
     1381 gtacattact ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc
     1441 gtatctcata ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt
     1501 gcctttataa cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt
     1561 tgagaagcta ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc
     1621 gacacccgtt gctgttgttg tatttgtact ggatacaatg gccaataaat tatcattgat
     1681 tgcatcagcc tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc
     1741 gttcaactga atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc
     1801 ggtacgatat tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc
     1861 agcattcacc agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact
     1921 acccgtagcg gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata
     1981 tattccttca atctcattta tcgactccac atccgtatat aaccgattac tttatttaag
     2041 acactgatag tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca
     2101 ctctattcta aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcac
     2161 cacaaatata gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg
     2221 tatttcttaa attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg
     2281 ccatcatgta cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac
     2341 cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct
     2401 tctgacaaaa tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg
     2461 gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa
     2521 aaagttcagg ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg
     2581 ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc
     2641 gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag
     2701 cttatcgatg cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta
     2761 ccggcgggta ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac
     2821 ggtttccatc cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc
     2881 accccgcgcg gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac
     2941 gccgtggtga ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc
     3001 gcaggttgca ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa
     3061 aatgccgatc tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc
     3121 aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg
     3181 ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc
     3241 ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa
     3301 tatcatgatc cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt
     3361 tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat
     3421 cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa
     3481 aatcgtcgcc ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc
     3541 cctttcccgg cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc
     3601 gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt
     3661 acgcacgccg ccgttcccct ttgcattggt ttagggttgg gttcgaaagg tctgttattt
     3721 gccggaatca tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa atttggcgtt
     3781 gcttacggca atgtttttgg tcatcgcggt tttacccatt cgctggtgtt tgcgtttgtt
     3841 gtcccgctct tatgtgtgct gattggacgt cgatggttca gggctgggct gattcgctgc
     3901 tggctgtttt taaccgtctc gttgctatcg cacagcttac tggattcggt aaccactggc
     3961 ggtaagggcg ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag
     4021 gtgattaaag tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg gcatcaggtg
     4081 attatttccg aattgatgtg ggtgtggttg ccggggatgc tactgatggg aatgttgtgg
     4141 tggcgcagac gataaccgga tgcgaaaact cgcatccggc aattgcgcaa ttacttacga
     4201 cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca
     4261 cgtgccgcat ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt
     4321 aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct
     4381 tccggttctt gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt
     4441 gcattcgccg ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac
     4501 agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct
     4561 tcaaacgctt cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcagtgtag
     4621 aggcgctcca gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagctggctg
     4681 cgatagtggc ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca
     4741 tcgcgcacgg taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc
     4801 attccggagt gcatccagta gttcacatac tgaccatcat gggcacaggt ggactgcgcg
     4861 atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg
     4921 ttacccagct gcttgcagtt catggccgaa cattcaatgt gccagccagg acgacccgcg
     4981 ccccacggag acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc
     5041 atccggttgc gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag ctggtcgaga
     5101 tcctgacgcg acagcacgcc ataagttgga tcggtcggga cgtcgaacat cacgtcgctg
     5161 ttgtccgcca cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct
     5221 gcgatatggt gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa
     5281 tctttgtgca tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca
     5341 ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta
     5401 tagccgagga aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga
     5461 ccgatatgac agagatcgta aacggtgatt ccacacacgt acatgccgac ttccccggcg
     5521 tgaataggct taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa
     5581 gattccgttt agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg
     5641 aatcagcata cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat
     5701 atgacccgaa ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt
     5761 ccacaccaat cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt
     5821 taaaaacttc ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt
     5881 tatcaacggc tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac
     5941 caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg gtacgctggc
     6001 aatggcacgt actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga
     6061 taacgacttc ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc
     6121 tgaagtggtt gacggcatgg acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtaa
     6181 cggtatgcac caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga
     6241 gtaatcgtgg cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc
     6301 accgccggtt ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt
     6361 cttggcgatc tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag
     6421 atggcggcag cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc
     6481 aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg
     6541 gaagaaaagg tgctcgaact ttatggtcgc cgggtgttga ttatgcatgg cgacacgctg
     6601 tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa
     6661 atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgcgcg
     6721 aacagcaaag aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgct
     6781 gtggtcagtg cgatggaaaa acatcaggtg caatggctaa tccacgggca tacccatcgc
     6841 ccggcggtgc atgaacttat cgccaaccag caacctgctt ttcgcgtggt actgggtgcc
     6901 tggcatacgg aaggttcgat ggtgaaagtc acggctgatg acgttgagct gattcatttt
     6961 ccgttttaaa aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc
     7021 tctttccgtg ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta
     7081 agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc
     7141 gactgggcta ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt cccgcaccac
     7201 gttgaagtgg tttctgctca ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc
     7261 gaagagaacg gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgccaggc
     7321 atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg
     7381 agcggtgtcg atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg
     7441 ctggcgattg gtaaagctgg cgcggcaaac gcggcattac tggcagcaca aattctcgcg
     7501 actcacgata aagagctaca ccagcgtctg aatggctggc gcaaagccca gaccgacgaa
     7561 gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg
     7621 ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct gtctggccgg
     7681 tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga
     7741 tcgaacgctg gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga
     7801 accgcgatgt gttcccgatt attgctgacc gtctgactca gaaacagctt ttcgataagc
     7861 tccacctgcc gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt
     7921 ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg
     7981 gtcaatggag tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt tacggtgaat
     8041 gtattgtcga gcagggcatt aacttctctg gtgaagtatc gctggttggc gcgcgcggct
     8101 ttgatggcag caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc
     8161 gcaccagcgt cgcttttccg caggccaacg cacagcagca ggcgcaagcc gaagagatgc
     8221 tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca
     8281 ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt gcataacagc ggtcactgga
     8341 cacaaaacgg tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc
     8401 cgttaccgca accagtagtg aataatccgt cggtgatgat caatctgatt ggtagcgatg
     8461 tgaattatga ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc
     8521 gtccggggcg taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgactg
     8581 cgacgctgga agccttaatc ccgctgctgc cgccagagta tgccagcggc gtgatttggg
     8641 cgcagagtaa gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct
     8701 tatccggctt accgctccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat
     8761 caggcatggt gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc
     8821 ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg gtttactacg
     8881 gcttctgaca taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc
     8941 aaatggcgct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca
     9001 gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc
     9061 ggcgagcaac gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc
     9121 cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa
     9181 ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa
     9241 atatttacca tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc
     9301 tggctgataa accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga
     9361 aacctcaatg cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct
     9421 ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagtacat ccagcggata
     9481 cggttctacc tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt
     9541 gccgtgaaca atcgccagcc gataagaacg ggccaggcgt gccagcgcgg gtacagcact
     9601 ggcgatattg cgatattgat tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt
     9661 gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc
     9721 cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc
     9781 gcaacagcgg tacgtttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg
     9841 aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga
     9901 agattgtcgg aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac
     9961 caggccgcca gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct
    10021 ttacccagcc agtgccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga
    10081 cgaaactggc gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggcccgaaa
    10141 agtccggttt cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc
    10201 ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga
    10261 atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg
    10321 aattgcgtgc ggcgaaatta cacattcccg tcaagctata gaagaaacgc tgccctggct
    10381 ttcctctttt ggcgctgacc caacgggtgg gatgacccgt ttactttatt cgccggaatg
    10441 gctggaaacc cagcagcaat ttaaaaaaag aatggcagca agtgggctgg aaacacgttt
    10501 cgatgaagtg gggaatttat acggtcgcct gagtggcacc gaatatccac aggaagtggt
    10561 tctgagcggt tcgcatatcg ataccgtggt taacggcggt aacctcgacg ggcaattcgg
    10621 cgcgctggcg gcgtggctgg caattgactg gttgaaaacg caatacggcg caccgttacg
    10681 tacggtcgaa gtggtggcga tggcagaaga agaaggcagc cgcttcccgt atgtcttctg
    10741 gggcagtaaa aatatctttg ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc
    10801 caaaggaaat agttttgccg atgcgatgaa ggcttgcgga tttactctgc cgaacgcccc
    10861 actaactccg cgtcaggcta taaaagcctt tgttgaactg catattgaac agggctgtgt
    10921 gctggaaagt aatgggcaat caattggcgt ggtgaatgca attgtcgggc agcgtcgcta
    10981 tacggtgacg ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg gttatcgtcg
    11041 tgatacggtt tacgctttca gtcgcatttg ccatcagtcg atcgaaaaag cgaaaaagat
    11101 gggcgatccg ctggttctga cctttggcaa agtagagccg cgcccgaata cggtaaatgt
    11161 ggtgccgggt aaaaccacgt tcaccattga ttgtcgtcat accgacgctg ccgtgctgcg
    11221 cgatttcacc caacagttag aaaacgacat gcgggcgatt tgcgatgaaa tggacattgg
    11281 tattgatatc gatttatgga tggacgaaga acccgtgccg atgaataagg agctggtcgc
    11341 caccctgaca gaattgtgtg aaagagaaaa actgaattac cgggtgatgc acagtggtgc
    11401 cgggcacgac gcgcaaattt tcgcgcctcg cgtaccaacc tgcatgattt ttatccccag
    11461 catcaacggg atcagccata acccggcgga acgcaccaat attactgacc ttgccgaagg
    11521 ggtcaaaacg ttggcactca tgctttatca acttgcctgg caggaataag gagtcataaa
    11581 tgggatattt aaataacgtc accggttacc gcgaagattt actggctaac cgtgcgattg
    11641 ttaaacacgg taatttcgca ctgttaaccc cggatggtct gataaaaaat attattccgg
    11701 actttgaaaa ttgtgacgcg acaatcctct ccacgccaaa gctgggtgcc tcttttgttg
    11761 attatctggt cacactgcat caaaacggtg gcaaccaaca gggcttcggt ggcgaaggca
    11821 ttgaaacgtt cctgtatgtg atctctggaa atatcactgc caaagccgaa ggcaaaacat
    11881 ttgccttaag cgaaggtggc tatctttatt gcccaccagg ctccttaatg acgtttgtta
    11941 acgcccaggc cgaagacagc caaatctttt tatataagcg ccgctatgtt ccggtagaag
    12001 gccatgcacc gtggctgttt tctggcaatg ccagcgaact ggaacgcatt cactatgaag
    12061 gtatggatga tgtgatcctg ctggattttc tgcccaaaga gttaggtttt gatatgaaca
    12121 tgcatatcct ctcttttgca ccaggtgcca gccacggtta tatcgaaaca cacgttcagg
    12181 aacatggtgc ctatattctt tccggtcagg gggtttataa cc
//
LOCUS       DAJTUI010000106        12072 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTUI010000106 DAJTUI010000000
VERSION     DAJTUI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12072
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.106"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="OO464_003301"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="OO464_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR6228338.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="OO464_003302"
     CDS             complement(2005..2928)
                     /locus_tag="OO464_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6228339.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3145..4488
                     /locus_tag="OO464_003303"
     CDS             3145..4488
                     /locus_tag="OO464_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6228340.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4713..6368
                     /gene="opgD"
                     /locus_tag="OO464_003304"
                     /gene_synonym="mdoD"
     CDS             4713..6368
                     /gene="opgD"
                     /locus_tag="OO464_003304"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6228341.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="OO464_003305"
     CDS             6508..6732
                     /locus_tag="OO464_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6228342.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="OO464_003306"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="OO464_003306"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6228343.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8306)
                     /locus_tag="OO464_003307"
     CDS             complement(7326..8306)
                     /locus_tag="OO464_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228344.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8430..9422
                     /gene="tehA"
                     /locus_tag="OO464_003308"
     CDS             8430..9422
                     /gene="tehA"
                     /locus_tag="OO464_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6228345.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9419..10012
                     /gene="tehB"
                     /locus_tag="OO464_003309"
     CDS             9419..10012
                     /gene="tehB"
                     /locus_tag="OO464_003309"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6228346.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10315..10971
                     /locus_tag="OO464_003310"
     CDS             10315..10971
                     /locus_tag="OO464_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR6228347.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="OO464_003311"
                     /pseudo
     CDS             complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="OO464_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11503..12072
                     /locus_tag="OO464_003312"
                     /pseudo
     CDS             11503..12072
                     /locus_tag="OO464_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3125 a         2825 c         3032 g         3090 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cttcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agtagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagacc
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat
     3001 attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcaga
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cacgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata
     8221 atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt
     8281 tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta
     8341 gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac
     8401 tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg
     8461 caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca
     8521 gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca
     8581 tctggggatt attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc
     8641 tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga
     8701 tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt
     8761 ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat
     8821 ctcaccctga agaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta
     8881 tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg
     8941 caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg
     9001 gagaattacc cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg
     9061 cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt
     9121 tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc
     9181 agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg
     9241 gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt
     9301 ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc
     9361 agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat
     9421 gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga
     9481 agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa
     9541 tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa
     9601 tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca
     9661 cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc
     9721 gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca
     9781 acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta
     9841 tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga
     9901 aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa
     9961 cggtaatcgt attaaactgc gtttcgccac gatgctggca cgtaaaaaat gacccggtaa
    10021 gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag
    10081 catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt
    10141 aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt
    10201 cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag
    10261 cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt
    10321 accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc
    10381 tctaaaatca cccagccaga taaatataac aattactctg atttaaaaga aacaacctca
    10441 gcaacaggta aacctgtttt acgttgggta gacccgagtt ttgatcaaag caaatatgac
    10501 agcatcgtct ggaacccaat cacttattat ccggtaccaa aaccttctac ccaggtaggg
    10561 cagaaagttc tggataaaat tttgaactat accaacaccg aaatgaaaga agcgatagcg
    10621 cagcgtaaac cagtggttac caccgctggg ccgcgtagtc tgattttccg tggggccatt
    10681 accggtgtag ataccagcaa agaagggctg caattctatg aagtggttcc tgttgcatta
    10741 gtggttgcgg ggacgcaaat ggctacaggc caccgtacca tggatactcg cctctatttt
    10801 gaaggtgagc tgattgatgc agcgactaat aaaccggtta tcaaagtcgt tcgtcagggc
    10861 gaaggtaaag acctgaataa cgaaagtacg ccaatggctt tcgaaaatat taaacaagtt
    10921 attgatgaca tggcgaccga tgccaccatg tttgacgtta acaaaaagta gttccagacg
    10981 cgccatcgtt agatggcgct ttttatccgg tgcgccgtaa aaccccatcc ttcagggcgg
    11041 ggatataagg cgcggttttc cacctgacca ggtgtttgct gttgttcaat gtattggcgg
    11101 atgacggata ttggcgcacc tccgcagcta ctggcaaaat agccaggact ccacaaaacg
    11161 cctttgtagt aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa cctacccgat
    11221 acgcctttga ggctgtttac cagactggat atcgccagtt tgggaggtta gttaattaac
    11281 aaatggacgt gatctggttc gccatccatt tcaaccagtt cagcttcaaa ataagcacat
    11341 acatttgaaa agtaagtgcg tagtttttct gtcgcgtcat ggtcaaaaat ctggcgtcgg
    11401 tatctggtga caaagactag gtgaacatgc atcaggaaaa cacaatgcct tccacgccgg
    11461 atatcggttt cttttttcac agaccaaagt atcatttgac ctgtgaaacg attacaggca
    11521 tttaaattcc agttaagacc aggtggtcaa caggagtgtg aaatgaggcg cttcgcaggc
    11581 gcatgtcgtt tcgttttcaa tcgtgctctg gcacgtcaga atgagaatca taaggccggt
    11641 aataaataca tcccttacgg gaaaatggct tcctggctgg ttgagtggaa aaatgccact
    11701 gaaacgcaat ggcttaaaga ttctccctca cagccattgc aacagtcact gaaagacctt
    11761 gagcgggctt acaaaaactt cttccggaag cgggctgctt ttccccgatt caaaaagcgg
    11821 tgacagaatg atgcattccg ctacccgcag ggcgttaagc tcgatcagga aaacagccgt
    11881 atttttctgc cgaaactggg ctggatgcgc taccggaata gccgtcaggt cacgggtgtt
    11941 gtgaaaaatg tcactgtcag ccagtcctgc ggtacgtggt acatcagtat tcagacagaa
    12001 agtgaagtat ccactccggc tcacccttca gcatcaatgg tcgggctgga tgctggcgtg
    12061 gctaaactcg cc
//
LOCUS       DAJTUI010000107        11990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTUI010000107 DAJTUI010000000
VERSION     DAJTUI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.107"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(335..979)
                     /gene="cheZ"
                     /locus_tag="OO464_003313"
     CDS             complement(335..979)
                     /gene="cheZ"
                     /locus_tag="OO464_003313"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR6228348.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(990..1379)
                     /gene="cheY"
                     /locus_tag="OO464_003314"
     CDS             complement(990..1379)
                     /gene="cheY"
                     /locus_tag="OO464_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR6228349.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1394..2443)
                     /gene="cheB"
                     /locus_tag="OO464_003315"
     CDS             complement(1394..2443)
                     /gene="cheB"
                     /locus_tag="OO464_003315"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR6228350.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2446..3306)
                     /gene="cheR"
                     /locus_tag="OO464_003316"
     CDS             complement(2446..3306)
                     /gene="cheR"
                     /locus_tag="OO464_003316"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR6228351.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3325..4926)
                     /gene="tap"
                     /locus_tag="OO464_003317"
     CDS             complement(3325..4926)
                     /gene="tap"
                     /locus_tag="OO464_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR6228352.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(4972..6633)
                     /gene="tar"
                     /locus_tag="OO464_003318"
     CDS             complement(4972..6633)
                     /gene="tar"
                     /locus_tag="OO464_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR6228353.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6778..7281)
                     /gene="cheW"
                     /locus_tag="OO464_003319"
     CDS             complement(6778..7281)
                     /gene="cheW"
                     /locus_tag="OO464_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR6228354.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7302..9266)
                     /gene="cheA"
                     /locus_tag="OO464_003320"
     CDS             complement(7302..9266)
                     /gene="cheA"
                     /locus_tag="OO464_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR6228355.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9271..10197)
                     /gene="motB"
                     /locus_tag="OO464_003321"
     CDS             complement(9271..10197)
                     /gene="motB"
                     /locus_tag="OO464_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR6228356.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10194..11081)
                     /gene="motA"
                     /locus_tag="OO464_003322"
     CDS             complement(10194..11081)
                     /gene="motA"
                     /locus_tag="OO464_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR6228357.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11208..11786)
                     /gene="flhC"
                     /locus_tag="OO464_003323"
     CDS             complement(11208..11786)
                     /gene="flhC"
                     /locus_tag="OO464_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR6228358.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11789..>11990)
                     /locus_tag="OO464_003324"
     CDS             complement(11789..>11990)
                     /locus_tag="OO464_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR6228359.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2744 a         3361 c         2913 g         2972 t
ORIGIN      
        1 caatccaagc ggttcggtaa cgaagatttt acgcactatt tttactaagc aggtaaagag
       61 tttagggccg gaactctgtc atttcttgca ccatgaactg acgaaaaaac gttatgaagt
      121 atggcatcac tacgatgtac tgcactacca gtcatcgtca ttttctcgtt cacaatttaa
      181 tgcattgata aatttactgg cgcaacaatc acgcttcgct gatctaaaat tatcaaacta
      241 caaataacca tcctccacgt actccctgaa cgatacccgc caccctgcta tcggcttggc
      301 atcatcgcgc aggccgaagt tcaggcaata caaatcaaaa tccaagacta tccaacaaat
      361 cgtccacctg atcctgactg gctaccacgc cggctttgct ggtatcgacc tgaggtccat
      421 taagcaaact ctggttttca cgttttggac gcgactcctg ttccgggatg ttttccaaca
      481 gcaccatcag caactggcgt tcgatctcct gaatgacatc catcatccgc ttaataacct
      541 gcccggtgag atcctgaaaa tcctgcgcca tcatgatttc cagcagttgc gcgttagtaa
      601 agctggtatg cgcgggtaca tctgccagaa attgtcgtgt atctgttacc agttcacggg
      661 cgtcggcaag gtcaatcgga tcggcaaacc agtcatccca acgttgggtt aacgcttttg
      721 ctgatttctc catttgatcc tgatgcggtt gtgacgcctc aacactgttc agcgcccgct
      781 ccgcagcctg ggcggtcatc tgcacaacat agtacaaacg atcgcgcgca tcggggatgg
      841 cttccgccgc ttcggcaatg gcctgaacca gccccagttc ccgcaaactg tcgcgcagca
      901 tatgcgtcag gctgccgatg cgcgcaatga tatcgccagc tgaatgctcg tcagcaggtt
      961 tgattgatgg ttgcatcata gtcgcatcct cacatgccca gtttctcaaa gattttgttg
     1021 agtttttcct ccagcgtcgc ggcggtaaat ggcttcacca catagccact ggcccccgct
     1081 tgcgccgcag caatgatgtt ctctttcttc gcttctgcag tcaccattaa cactggcaat
     1141 gccgacatcg cgccatccgc acgaattgtt ttcagcaatt ccaggccatc catattgggc
     1201 atgttccagt cggagataac aaatccataa ccgcctgcct gcaacttatt gagagcgtcg
     1261 acaccatctt ccgcttcctc aacattattg aatcccagct ctttcagcag gttacgcact
     1321 atgcgtcgca tggtggaaaa gtcatccaca accaaaaatt taagttcttt atccgccatt
     1381 tcacactcct gatttaaata cgtatcgcct gtccggcact aattgttgcc aacatttgct
     1441 ggcttacctg gctaagatcg accacttcgc agacaccacc catattgatg gcctcgcgcg
     1501 gcatgccgaa caccacgcaa cttgcttcgt tttgcgcaag ggtccatgcc cccgcctgac
     1561 gcatcgccaa cattcccgcc gcgccgtcgt tgcccatacc ggtcaggatc accccaaccg
     1621 cattacgccc cgcctgtttg gcgacagaat ggaacaacac atctaccgaa ggccgatgac
     1681 ggttaaccgc cgggccgtcg tgaattttga tttgataatt tgcgccacta cgcgccagtt
     1741 ccatatgccg atcgcccggc gcaatatagg catgccccgg caagacacgt tctccgtctt
     1801 cggcttcttt aaccccgatc tggcaaagct tattaagtct gtcggcaaaa gagcgggtga
     1861 aaccgggcgg catatgttgg gtaattaaca atgccgggct ggaaagcggc aacggttgca
     1921 gtacgtgacg aattgcctca gttccacccg ttgaagcacc aatcgcaatc agtttttcag
     1981 aactcaacaa cggccctgcc ttcagcgttg tcggtgctga caatggctta tgtgctgcaa
     2041 ggctcgcctt tgctgccgta cgcacctttt cagcaatcat ttcgctatac gccagcattc
     2101 cttcgcgaat acccagttgc ggtttggtga caaaatctat tgcccccagc tccagcgcgc
     2161 gcagcgtgac ttctgatcct ttgccggtca aggaagaaac catcacaacg ggcattggac
     2221 gcaaacgcat taatttttcg aggaaatcca gtccgtccat ccgcggcatt tcaacatcca
     2281 gcgtcagcac atcgggattg aatttcttaa tcaagtcacg cgcaaccagc ggatcaggcg
     2341 cggtcgccac catttccatg tcgctatggc tgttgatgat ttctgtcatg atctggcgca
     2401 tcagtgccga atcatcgaca gataacaccc tgattttgct catcgttaat ccttacttag
     2461 cgcatacacc gtctgaccac gcagcgtgaa gcggcgctcg aggtggctaa agttttcaga
     2521 gtgacccgca aacagcaatc cgtcgggttt aaggagcgga acaaagcggc gcaaaatctc
     2581 ctgctgggta gtttgatcga agtagatcat gacgttacga cagaagatcg catcaaacgg
     2641 ccccggcacg gtgtactgtt tcgccagtaa attcagcggg gcaaaatcaa catagttcgc
     2701 cagctcctga cgcacgcgta ccagcccttc atgcggtccc gtccctcgca tgaaataccg
     2761 ttgcagttgc tgcggcgtca ggtttttcaa ctcttcatgg cgatagatac cgcttctggc
     2821 tttttccagc acttcggtgt cgatatcact ggcaaacact ttccagcgtc cgggcacggt
     2881 gcccaatgtg tcagccagag tcatcgcaat gctgtacggc tcttcgccgg tcgaagccgc
     2941 cgcgctccat acgcgatcct cgccagaacg gcgacgtgcg tgatccgcga gcagagggaa
     3001 atgatgagcc tcacggaaaa atgccgtcag attcgtggtc agcgaattga taaacgcctg
     3061 ccactcaccg ctgtgctgat tagattccag caagttcaga taatgaccga aatccgtcag
     3121 tcccagagaa cgcaaacgac gaaccagtcg gttgtaaacc atgtcgcgtt tatggtcagc
     3181 cagaacgatc ccggctcgtt gatagatcaa ttgacttatc cgccgaaaat gcgcgtcgga
     3241 aagcgccagg cgctcggtca tctgtaacaa taaagacgtt tgcccacagg gcagagatga
     3301 agtcatagcg ccttctcaat cacttcagga taccactggc gcaatttgta actgcgccga
     3361 ctcatgtcgt gctacttcat gttcttcaag ggtaaatacc gccacacgcg acgaaagatg
     3421 gtcggcctga ttcgccagtt gttccgttgc caccgccgct tcttctacca gcgaggagtt
     3481 ctgctgagtc acctgatcca tctggctgac agcctgtgca acctgctcaa tcccccgccg
     3541 ttgttcttcc gacgccgaag caatttctcc cataatgtcg ttcacgcggg tcaccgaact
     3601 gacaatatcg gtcatggtcg cggcggcgtt attcaccagt ttcgaaccct gctggacacg
     3661 attgactgac tcttcgatca gccctttgat ctcttttgct gcctgcgcgc tacggctggc
     3721 aagattgcgc acttcacctg ccactaccgc aaaaccacgc ccctgctctc cggcgcgagc
     3781 cgcttccact gccgcattca gggccagaat attggtctgg aaagcaattc cgtcgataac
     3841 gctgataatg tcgccaattt tttgcgagct ggtggcgatc tcctgcatgg tgtgagtcat
     3901 ggtactgacc tgaacaccgc ccgcctgcgc cgttgtcgcg gcattttttg ccagttccga
     3961 cgcctgtcgt gcgttatcgg cgttttgccc taccgtggcg gttaattgct ccatactggc
     4021 ggccgtttgt gccagcgatg ccgcctgctg ttcggtacga cttgagagat cgttattgcc
     4081 tgcgacaatc tccgcgatcc caatgtgcat ctcatggctt cccttacgca catcactcac
     4141 cgtcccacgc aaagcctgct gcatggtctt caggctggca aaaatggcgg tgatctcatt
     4201 acgaccatat accgcaatcg gacgcgccag attacccgca gcaatgctgt caaaatggct
     4261 accgataatg gccagtggtt gaacaatcat cttgcgcgtc caccacagcg cactgctgat
     4321 gtagatcgcc gcaacaataa tcatgctgat aaacaccagc gccgaaatct gatagttacg
     4381 ctggctttgc gcactggcgg cttccagcac atggttgatc tccagctgcc aggcctcaaa
     4441 gttaacgtca aacgcattct gtgatccctg caccggcgca gtgaggaaat ccgaaagttg
     4501 attactttca agccaggtcg cctgatgttc gagatcgttg tgccagcggg caaaactttt
     4561 ctccgtctct ttttgcaatg ccctgacgtg ctcgttgccc gcagtcatcg ccataaaact
     4621 tttaaacagc gtggtggatt gcgtcagact ggcgcgcgcc gtcgtcatca acgttttaat
     4681 gtcatccgcc ggatagctaa gcgctgtcag agtgcctgct ttgttcagcg cggtactggc
     4741 ctgtaacatt accgcccgag tttgcgctaa tgccgcacgt tgctgattgc tctgctcgcc
     4801 gtgattcagt cgttgcaaat cgtcgcgaaa tgcccaaaaa gacatgccgt tactgccaat
     4861 ctgcaagatc ccgcagagaa tcaaaattaa aaacagcgtg gtcgaaattc gaatacgatt
     4921 aaacatcaac gctccccatc aggcggcaat gaccgcgtta gtaaaaactc gtcaaaatgt
     4981 ttcccagttt ggatcttgtt cagtaattcg cagtcgtggc tgtgccggtg gttgctcact
     5041 ggcaggacgg gatggtgttt gcggtttatt ggtgagtggg ctggctgcca gacggaacgc
     5101 ggaaaccgct tgcgttaaac gactcgcctg ttcttccagc gcagctgcgg cggcagctga
     5161 ttcctgcacc agcgatgcgt tctgttgcgt gacgcgatcc atttccgaaa ccgccaatgc
     5221 gacttgatcg atgccacggc tctgttcatc cgatgccgat gcaatctcgc ccataatgtc
     5281 agtcacgcga gtgacagcat tgacgatatt gttcattgtt tccccggcgc tttcgaccag
     5341 caccgaaccg gtatcaatgc gcgagacgga gtcttcaatg agggctttga tctcttttgc
     5401 cgccagggcg ctgcgactgg caagattacg cacttcaccc gccaccacgg caaaaccacg
     5461 gccctgttca cccgcacgcg cggcttcaac cgcggcattc agcgcgagga tattagtctg
     5521 gaaggcaata ccgtcgataa cgctgataat gtcggcaatt ttcttcgaac tatcggcgat
     5581 ctcatgcatc gttttcacta cgccatccac cactttgccg ccgtgctggg cggtgtcgga
     5641 ggcactttgc gccagttgcg aggcctggcg ggcgttatcg gcgttttgct tcactgtcgc
     5701 ggtgagctgc tccatgctgg cggcagtttc ttccagcgcg gatgcctgct gttcagtacg
     5761 ggaggaaaga tcggtgttgc ccgccgcaat ttcacgggta ccggcataga tggcatctga
     5821 accttcgcgg acatgagtga cggtgtcagt caaagagcgt tgcatatgtg aaacgctctg
     5881 cgccaggtca cccatttcac tgcgcccgtc aatggtcagg gtattcgcca ggttaccacc
     5941 ggcgatttcg caaatgtgag caataatttt tgccagcgga gtaagcaaca tacggcgaat
     6001 gccgtaccac gccaccagca gaatcaatac caccaccagc gcgataaccg ccagttgcca
     6061 ctgggcaaat cggtaatcat ctgcgttgtc agtgacgata tcgcgataca gtttttcact
     6121 gctgagggcg tactgagcaa acgcttcgcc cattgcattt tgcattccct gggttggctg
     6181 agcgaaataa gctccagtat tgccataatc aagataatca atcagttcag ttaacgctgt
     6241 gtaatagttt ttatattttt catcaaaatt acggctggta gcgaccattt caggtaacgg
     6301 tgccatgctt ttgaattttt tataatgcgt cgcggcctgg gccaatgttt tcctggcgct
     6361 atcgagcaat tcaactttgg cgttactttg ttgattggag gaatccatca tcatccgcac
     6421 cgctgaacga ctcaggttaa tgcgcgtttg cagcattaaa tcccaggttg acgtcagctc
     6481 gccctgctgt tcccgtaatt gattggaaac cacaaagctc ttctggctat ggtgaaggga
     6541 agaaaaaaac aaactgccgg aaataagctg taacagtgcg aataccccca gcaccattac
     6601 caacagcgtg actacgcgga tacggttaat cataaggcac cttcctgaaa acaagttgat
     6661 ctcgttatcg gcaagaaggg gggaaacttt attgctgatg ccacccgccg cgaaattgaa
     6721 ataaaaaacc cgatgcgcag atcatcgggt tcatttcaat tgaggaaatc gggagaatta
     6781 cgccacttct gacgccgcgc tatctaacag cgccatctct tcgctgttca gcagtttttc
     6841 gatgttcacc agaatcaaca tacggtcgcc cagtgcgccc agtccagtga gatattctgt
     6901 tgaaagcgtc acggcaaact ctggtgccgg acgaatttgc tccgccgtca atgaaagcac
     6961 gtctgaaacg ccgtcaacca cgatgccgac cacccgctgt ccgagattca ggacgataac
     7021 taccgtgttg tcgttatagt ccacatccac ctggctgaac ttaattcgta agtcaacaat
     7081 cggcacaata acgccgcgca gattcgtgac gcctttgata aacgctggcg tgttcgcaat
     7141 ccgtgttacc tgatcgtagc cacggatctc ctgcactttc aggatatcaa taccgtactc
     7201 ttcatcacca agggtaaata ccagaaattc ctggcctgac ggctcgctgg ccagctttgt
     7261 tacattcgtc ataccggtca tattgttacc tttttactca ttcaggcggc ggtgttcgcc
     7321 atacgttgtt cgcggtttat cgcctgcaag gcggagacat caacaatcag tgccacgctg
     7381 ccgtcgccaa gaatggtcgc agcagaaatg ccggggactt tgcgatagtt actttcaagg
     7441 tttttaacca caacctggtg ttgaccaatt aattgatcca ccagcaaggc gtagcggcga
     7501 ccgccacttt gtaagatcac cacaattccc tgggtggctt cggttttcgc gcccgcgacg
     7561 ttgaacactt tccacagttc gacgatgggc agatattcac cccgcacttc cagcacccgc
     7621 tcgccgccgg ccagtggatg gagatcggct tcacggggtt gcagtgattc cataacagca
     7681 ttcagcggca gaatgaaaac ttcatccgca acgcgtacgg acatgccgtc gaggatggcc
     7741 agcgtcagcg gcagtaaaat gcggatcgta gtgccagtac cctgcttcga ctggatttcg
     7801 acatgaccgc ccatctcctg gatattacgt ttaacgacgt ccatgccgac gccgcgcccg
     7861 gagacgtcgg tgacctgctc tgccgtggag aagcctggtg caaatatcag cattgcaact
     7921 tcgtcgtcgc tcatgttttc gctgacagtc aaaccttgcg aggccgcttt tgccagaatt
     7981 cgctcacggt ttagccccgc cccatcgtcg gtcacttcaa tgcaaatgtt gccgccctga
     8041 tgttcggcag acagaattaa atttccgacg ctgtttttac ctgcggcgag ccgtttttct
     8101 ggcagttcaa taccgtgatc gaggctattg cgtaccaggt gggtcagcgg gtcgataatg
     8161 cgttctatca ggctcttgtc gagctcggtg gaactgccca ccagcgtcag ttctacctgc
     8221 ttgccgagtt ttcccgccag atcacgcacc agccggggat agcgactaaa gacatattcc
     8281 atcggcatca tgcgaatcga catcactgat tcctgcaaat cacgggcgtt acgttgtaac
     8341 tgccccatgc tggtaatcaa atcaccatga ttaaccgggt ccagttcgct ggaacgctgg
     8401 gcaagcatgg actgggtgat aaccagctcg ccgacgaggt taattaattg atcaaccttt
     8461 tctaccgcta cacggatgct ggtggattca ctgctgcgcg tcgttttttc ccgctccacg
     8521 cggccggttg gcgcttgttc ggctgccagt ttaagcacag gtggggtagc tatttttggc
     8581 gaaacttcta ctgtttcaaa ggtaatctga tcggcttcaa tcacaaaaca gagtaccgct
     8641 gtgatgtcat cttcggcgat atcgcccggt aatattgccg agagagaatc cgcccctttc
     8701 accacgtcat ttaacgttgt cagatgcccc agctcttctt ccagcaggtc gacttcgccg
     8761 gccttcaggc gcgaaaggat aattcgtcgc ggcaactgac tgcgactttg ctcatcttgc
     8821 ggttcacttt tggcaaccac acttaatcgg gtcactgcgg atggcgtttc gcctttcgct
     8881 tctaatgcca gttgacgcaa ggcctggcag atataatcgt agctggcggc atccggctct
     8941 tgcgactgtt tataagcgtc gagctgttct tgcatgatgt ccttcgtttc caaaaacaga
     9001 ttgataatgt cggtgttgag ttgcatctca cctcgtctgg cttcatcgag caggttttcc
     9061 atcagatgcg tggtttcctg caaaacgctg aagccaaaag ttcctgcccc tcctttgatc
     9121 gagtgggcag cccgaaagat ggcattcaat tgttcggcat ctggcgcttc cggctgcaaa
     9181 accagcagat gctgctccat atcagccaac agttcgtccg cttcatcaaa aaatgtctga
     9241 taaaaatcgc ttatatcgat gctcacgctg tcacctcggt tcggctgatg gcattgtggg
     9301 aacactgacc tgtggtgcaa cctcaggttt ttctagggcg cttactggct cattctggct
     9361 ttcggcgttt tcatgcaaaa tggcctgttc ggcttgtttg ttcagtacca acaggctgat
     9421 gcgacggttg acggcatcat caggtccgcg atcgcttaag cgcatcgttg ccgccatgcc
     9481 gacgacacgt aacactttgc cgctatccaa ccctccgacc atcagttcgc ggcgggatgc
     9541 attggcccga tcggcagaaa gctcccagtt gctgtaacct ttctcaccgc tggcgtaggg
     9601 aaaatcatcg gtatgacctg aaaggctaat acggttggga ataccgttca gcacaggcgc
     9661 gatggcgcgc agaatgtcgc gcatataggg ttcgacatcg gcactgccgg ttctaaacat
     9721 cgggcgattc tggctatcga tgatctgaat acgtagacct tcctggacca gatcgatttt
     9781 gagatggaga cgtaacgccc gcagtttcgg atcggactct atcaactggt cgagatcacc
     9841 ccgcaatttc cgcaatcgac tttgctccat gcgttttttc agctcttcga tgttcggctg
     9901 cttattcact tccccctggc tttgggtgta atcatcacca ccgccgggaa ttgggctttc
     9961 actattggaa atgcgatcgc cgcccgtaac cgctgtcgcc agtggagtcc ggaagtactc
    10021 cgcaatctga atcaactctt ttgggctgga gatggagatc agccacatca ccagaaaaaa
    10081 ggccatcatc gcagtcataa agtcggcata agcaatcttc cacgatccat gtgctgcccc
    10141 gtggcttttg gctttgcgtc gtttgacgac aataatcgga tgcgcttgat tcttcatgct
    10201 tcctcggttg tcgtctgttg ttgcggattt ttcaccgcac gcacatgctc ttccagttca
    10261 ataaacgacg gacgttcgct ggaatagagc gttttgcgac caaactcaac ggcgataggc
    10321 ggtgcgtaac cgttcagatt agaaagcaga gtgactttga cgcactgcat cattttgctg
    10381 gtttcggcgc ttttctgacg taaaacactc gctagtgggg aaataaatcc gtaagccaat
    10441 aaaataccga ggaaagtccc caccatcgca tgtgcgataa gcgcccccag ctcggcggca
    10501 ggacgatcgg ctgaacctaa cgcgtgaacg ccccccatta cagccgcgac aataccaaac
    10561 gccggaagtg agtccccgac cagcgccaga ctgttcgccg ggacttctgc ctcgctttcg
    10621 tgcgtctcaa tctcttcatc catcagggct tcgatttcga aggtgttcat gtgaccgctg
    10681 ataatcaggc gcagataatc gacgataaaa tcaagcatga cgctatccgc gaggatgcgt
    10741 gggtagctgg cgaagatctc gctctcacgg ggattttcaa tatcacgttc cagcgaaaac
    10801 atccccatct gccgcgattt cgccatcaac cgataaagca gagccagcag atccatatac
    10861 attgctttgg tgtatttgga gcgacgaaac agcaacggca gcgctttcag cgtgccttta
    10921 atcgctttgc cattattgcc gacgataaac gacccaatcc ctgcaccggc aataatcacc
    10981 agttcagcgg gttgatagag tgctccaagg cttccaccgg tcatcaaata accgccgaaa
    11041 actgtaccga gaacaaccag gtaacctaat aagataagca cgacatcatc cttccactgt
    11101 tgaccatgac aggatgttca gtcgtcaggc gttaacgcgc gattggggaa aaaaaaagca
    11161 gcggtacgtc gttaccgctg ctggaatgtt gcgccacacc gtatcagtta aacagcctgt
    11221 actctctgtt catccagcag ttgtgggata atatcggcag gattctggga aagtttacgt
    11281 ctttttactg cccgggatgg cggttgacat aagctgcagg caaagctgcc aacaggctgg
    11341 tgagcgtggg taataaaatt gccgccgcag cagttgcagc tggaaagttg cagtaatcca
    11401 ctttcaacaa accgcaccaa tgtccaggca cgggttaatg ccagcagtgg tccttcttct
    11461 gcttgtgggc actgttcaag gtataaacgg taggctttga tcaccgcatc gacgccatta
    11521 cacaaaccgg ttttcagtaa aaactgccat gcattacaga acatcgaagc atgaacgttt
    11581 tgttcccagg tcataaacca gtcggttgag aatggcagca tgcctttcgg cggtgggctt
    11641 ccgcgcagtt ctttataaag ttttatcagg cgtccgcgac ttaactgtgt ttcgctttcc
    11701 agcatctgca aacgagcgcc cagggtgatc aattccattg ccagctgaat atcccgcgct
    11761 tcctgaacaa tgcttttttc actcatgatc aggccctttt cttgcgcagc gcttcttcag
    11821 gctgattaac atcattcagc aagcgtgttg agagcatgat gccggtatga atttgctgga
    11881 gatcgtcaac gcgggaatct tgcgtcaact gagtaatcgt ctggtggctg tcaaaacgga
    11941 agtgacaaac cagctgattg gtttctgcca gcttaaccat ttgcggaaga
//
LOCUS       DAJTUI010000108        11773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTUI010000108 DAJTUI010000000
VERSION     DAJTUI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.108"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            116..985
                     /locus_tag="OO464_003325"
     CDS             116..985
                     /locus_tag="OO464_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR6228360.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1188..1541)
                     /locus_tag="OO464_003326"
     CDS             complement(1188..1541)
                     /locus_tag="OO464_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6228361.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1679..3325)
                     /gene="groL"
                     /locus_tag="OO464_003327"
     CDS             complement(1679..3325)
                     /gene="groL"
                     /locus_tag="OO464_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6228362.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3369..3662)
                     /locus_tag="OO464_003328"
     CDS             complement(3369..3662)
                     /locus_tag="OO464_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6228363.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3938..5194
                     /gene="yjeH"
                     /locus_tag="OO464_003329"
     CDS             3938..5194
                     /gene="yjeH"
                     /locus_tag="OO464_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6228364.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5210..5686)
                     /gene="fxsA"
                     /locus_tag="OO464_003330"
     CDS             complement(5210..5686)
                     /gene="fxsA"
                     /locus_tag="OO464_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6228365.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6023..7459
                     /gene="aspA"
                     /locus_tag="OO464_003331"
     CDS             6023..7459
                     /gene="aspA"
                     /locus_tag="OO464_003331"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6228366.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7577..8878
                     /gene="dcuA"
                     /locus_tag="OO464_003332"
     CDS             7577..8878
                     /gene="dcuA"
                     /locus_tag="OO464_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6228367.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8994..9332
                     /gene="cutA"
                     /locus_tag="OO464_003333"
     CDS             8994..9332
                     /gene="cutA"
                     /locus_tag="OO464_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6228368.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9308..11005
                     /gene="dsbD"
                     /locus_tag="OO464_003334"
     CDS             9308..11005
                     /gene="dsbD"
                     /locus_tag="OO464_003334"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6228369.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11042..11617
                     /locus_tag="OO464_003335"
     CDS             11042..11617
                     /locus_tag="OO464_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6228370.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2826 a         3042 c         2901 g         3004 t
ORIGIN      
        1 atagagtgag cacggctatt tatgttattt taacaactat tactaccaag cccatttatc
       61 tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa cctacatggc
      121 cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt tcattgcact
      181 ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt ctgtcatgga
      241 actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata aactggatgc
      301 tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa tggcagaaaa
      361 catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc gggttaacac
      421 gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc tacatgacgg
      481 tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc gagcaatcat
      541 tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct cactgttaga
      601 tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat atgacaccta
      661 ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc tttaccgctt
      721 taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc gtgaagcaac
      781 gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc cacgactgaa
      841 aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga ataacgccca
      901 accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac aatccaccag
      961 caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg gagccatcca
     1021 gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa acgccttatc
     1081 cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc ctgataagcg
     1141 aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca gttcgggcac
     1201 ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa cattccctgc
     1261 gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg gttgcgcaga
     1321 tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga aagccagttg
     1381 ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg ctcgtccaca
     1441 atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc cgccatcagt
     1501 agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc cataatcaat
     1561 gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga tgaccaaaag
     1621 aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg cagggcaatt
     1681 acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct aagtcagctg
     1741 catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg ccagccacag
     1801 aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata cccatgtcga
     1861 tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg ccgcctttaa
     1921 cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga cgcagcggag
     1981 cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc tgaccacgca
     2041 ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca accacgcctt
     2101 cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt ttctctttca
     2161 tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc agtttcgcta
     2221 cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc tgctgacgga
     2281 tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg atagtggtgg
     2341 tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt gctttttcca
     2401 gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg atatcctgca
     2461 gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact ttcacgatgc
     2521 cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct tcagcgatga
     2581 tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt tcgcggatgt
     2641 tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact gcgccagttt
     2701 ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata ccttcaacca
     2761 cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct tctttaccga
     2821 ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta gcggagatgg
     2881 taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct ttcagttctt
     2941 caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg ttcatgcccg
     3001 cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt gcagtggtgg
     3061 taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc tgcgcaccca
     3121 tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca tctttggtga
     3181 tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga ccgagggtaa
     3241 ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga gcgtcgttac
     3301 cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg ttcagtgtcg
     3361 tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat caacacttct
     3421 tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac gatgtcgcca
     3481 actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt gccgacagcc
     3541 agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat gccgccagca
     3601 gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg acgaatattc
     3661 attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg agcttacgtg
     3721 gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa gggggaaaaa
     3781 gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct cacatttcat
     3841 cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga aaatctacgc
     3901 agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca aacaagaact
     3961 ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg gcgtgtttgc
     4021 cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt ggcccgtttt
     4081 gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc actatcccag
     4141 cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg agcgagtcac
     4201 cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac aaattgccgc
     4261 cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg cagaactcgg
     4321 tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg ctaatctaca
     4381 aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg cgggcgatat
     4441 caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg ggttatttgc
     4501 tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc atcttgcctc
     4561 ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc tgctgctggc
     4621 aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct atggtgaaca
     4681 aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg taggagcgtt
     4741 atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca tttatataca
     4801 gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact acctggcacg
     4861 cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct gctgcgtggt
     4921 gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta tttatgccaa
     4981 tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat tattgcaagg
     5041 tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac tggcaatggt
     5101 cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt ttctgccaaa
     5161 acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat taatcctggc
     5221 gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac tcaccatcaa
     5281 aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga gaaaagcgca
     5341 aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa agcagaagac
     5401 cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata atcagcgaaa
     5461 cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc tgcatcagca
     5521 cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac gtgaatataa
     5581 ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag attgaaatct
     5641 caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt ttctcctgta
     5701 atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca gcttatattg
     5761 tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa tcccaaagcg
     5821 gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa gtgatccaga
     5881 ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg caggggataa
     5941 tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc aaatcattgg
     6001 cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag atctgttggg
     6061 taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag cgattgaaaa
     6121 cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg gtatggtaat
     6181 ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta aaagtgtagc
     6241 gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca tggatcagtt
     6301 cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca ccaacgaagt
     6361 gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc agtacctgaa
     6421 cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga ccggtttccg
     6481 tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac tgcgtgaagg
     6541 ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta cccagctgca
     6601 ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc tgctgaaaga
     6661 agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc ttggcgcaac
     6721 agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag tgaaaaaact
     6781 ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag cgacctctga
     6841 ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga agatgtccaa
     6901 aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga acgagatcaa
     6961 cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc cggttgttcc
     7021 ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg ttaccatggc
     7081 agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc aggccatgtt
     7141 cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat gcattaacgg
     7201 catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg gtatcgttac
     7261 ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa tctgtgccga
     7321 aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg aagcggaact
     7381 tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa aacgctatac
     7441 tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac gtcagatgac
     7501 gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa cttgttaaaa
     7561 aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg cgatcttctt
     7621 gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg tgctggttct
     7681 tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca ttatcatggc
     7741 ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc tggttcatca
     7801 gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac cgatcgtgac
     7861 ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac tgccagttat
     7921 cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta ctgcagtggt
     7981 atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt acatgtcttc
     8041 cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca tcccgtccac
     8101 cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact ccaaactctc
     8161 tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc gcggtgaaaa
     8221 gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc tgggcgtagt
     8281 tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg aaaaaccgct
     8341 gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc tgaccaccgt
     8401 tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag caggtatgag
     8461 cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt ccaacaacat
     8521 cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc tgctggccgt
     8581 catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa aagcactgat
     8641 gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt tcgctgcggt
     8701 gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac agatggatga
     8761 cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca tcccgggtac
     8821 tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca tgctgtaatg
     8881 atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc cttcccccgt
     8941 ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg tttatgcttg
     9001 atgaaaaaag ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg gatgaagcga
     9061 cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc gcgaccttga
     9121 tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa tacgaagtgc
     9181 agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc ctgaagtctc
     9241 atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga gacacagatt
     9301 acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca cttccgtttt
     9361 tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc aagcctttgc
     9421 ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca aagacggtta
     9481 ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg ccgacgagca
     9541 gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga tttaccgcga
     9601 tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt taactgtcac
     9661 ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa ccgttccgtt
     9721 aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc agcaagagca
     9781 gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta ttggtatcgc
     9841 ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc tgggtggtaa
     9901 acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc aggggatggc
     9961 actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt tccaggcggc
    10021 gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc tggcgatgtc
    10081 aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc tcacgttgat
    10141 gagcaatcgc caacagggcg gctcacctag cggtgtgttt gttatggggg cgattgccgg
    10201 actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt atatcgccca
    10261 aagcgggaac atgtggccgg gcggcggcac gctttatctc tatgcgttgg gcatgggcct
    10321 gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg gcccgtggat
    10381 ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct tcctgctgga
    10441 gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg tcgcattctt
    10501 tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg tggtgcaaat
    10561 aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg catttggtgc
    10621 gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa cggtagatga
    10681 gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc tttatgccga
    10741 ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc aggtgcaaaa
    10801 agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg cacaagatgt
    10861 ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt ttgacggaca
    10921 aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa ccttcagcgc
    10981 acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg aggaaaatag
    11041 cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac aagggattgc
    11101 caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg agctacgccg
    11161 cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta gccaacagat
    11221 agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac aaaagctgct
    11281 ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg gctgtctatt
    11341 tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac tggccgatca
    11401 gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg aagttgacga
    11461 tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa gccgaatgct
    11521 ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag atatcctgcg
    11581 cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa aagaagcgat
    11641 ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg agatgtgcag
    11701 attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag gggattgaaa
    11761 atccccgtgt cct
//
LOCUS       DAJTUI010000109        11514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTUI010000109 DAJTUI010000000
VERSION     DAJTUI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.109"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            38..778
                     /locus_tag="OO464_003336"
     CDS             38..778
                     /locus_tag="OO464_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR6228371.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            837..1223
                     /locus_tag="OO464_003337"
     CDS             837..1223
                     /locus_tag="OO464_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6228372.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1232..1561
                     /locus_tag="OO464_003338"
     CDS             1232..1561
                     /locus_tag="OO464_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6228373.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1533..4574
                     /locus_tag="OO464_003339"
     CDS             1533..4574
                     /locus_tag="OO464_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6228374.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4574..4903
                     /locus_tag="OO464_003340"
     CDS             4574..4903
                     /locus_tag="OO464_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6228375.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            4903..5601
                     /locus_tag="OO464_003341"
     CDS             4903..5601
                     /locus_tag="OO464_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6228376.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5606..6349
                     /locus_tag="OO464_003342"
     CDS             5606..6349
                     /locus_tag="OO464_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6228377.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6286..6888
                     /locus_tag="OO464_003343"
     CDS             6286..6888
                     /locus_tag="OO464_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6228378.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            6949..10428
                     /locus_tag="OO464_003344"
     CDS             6949..10428
                     /locus_tag="OO464_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6228379.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10496..11095
                     /locus_tag="OO464_003345"
     CDS             10496..11095
                     /locus_tag="OO464_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6228380.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11117..>11514
                     /locus_tag="OO464_003346"
     CDS             11117..>11514
                     /locus_tag="OO464_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6228381.1"
                     /translation="MTARWFRRAFCGVNMAVKISGVLKDGAGKPVVNCAIELRARRTS
                     PTVVAHVVATCVTDNNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNA
                     FLNAPKDGDLRPEVMKRFEEMVAQAQQSAGA"
BASE COUNT         2694 a         2717 c         3703 g         2400 t
ORIGIN      
        1 acgtaccaga tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc
       61 aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc
      121 gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg
      181 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
      241 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
      301 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
      361 tcgtatcaaa tatcccaacg gcactgttga tgttttccgc ggctggctga gctcactggg
      421 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
      481 tccgtatctg gcagaggaag gcactgaaac cgtgggtgtt accggactga cggtggcacc
      541 ggcatctgtc agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga
      601 cggggccagt gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgac
      661 cctgagcggg cttgtggcca cggtgaaagg cgtgaagcag ggcagtgtca gcatcgtggg
      721 catgacctct gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca
      781 ggacgatact catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt
      841 ttctgaaaca ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg
      901 ggctgcagag aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac
      961 aggagggaga actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata
     1021 tcaatgcctg gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga
     1081 cgctttgcgc atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga
     1141 gggttctgtc gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg
     1201 cgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga
     1261 gttccggcgg gcagactggc ggcggatgct gtcggatatg tcggccactg agcttggtga
     1321 gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc
     1381 ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agcgatgctg cggtggctga
     1441 tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca
     1501 tcttggggaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg
     1561 atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga
     1621 atggtgcagc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc
     1681 agactcaggc ggcccggcag acaatgcagg cggcttcttc ggctgcaaca gcagcatcag
     1741 cccatgcgca gacggtggag aagaatgcac gggctcatga acgcatggcc cgtgaggtgg
     1801 aacaaacccg tctgcgcgtg gatgccctga atcagaaaat gcgcgaggaa caggcgcagg
     1861 ccagggcact ggcggaggcg caggataaag cggctgccgc cttttatcgc cagattgaca
     1921 gtgtgaaaca ggccggtgcg gggcttcagg aattacagcg tattcagcag cagatccgac
     1981 aggccagaaa cagtggcggg gttggtcagc aggattatct ggcgctgatt tcggagatca
     2041 cggcgaaaac ccgtgccctg acgcaggcag aggaacaggc cacccggcag aaagcggcgt
     2101 ttatccgcca gcttaaagag caggcaaccc gccagaatct gtcgtcttct gagttgcttc
     2161 gcgccagggc ggctcagctg ggggtaagca gtgctgcaga agtgtatatc cgcaaaatgg
     2221 agcgggcagg aaaagccaca cattcgctgg ggctgaaaag tgcggcagcc cggcgggagc
     2281 tgggggtgtt aatcagtcag atggcgcgag gcaattttgg tgcgctgagg ggatccggga
     2341 taacgctggc taaccgtgcc ggatggacag gcgcactgat gtcgccgaaa ggcatgatga
     2401 ctggcggcgt tattggcgga cttgtcgcgg cggtcctggg tctgggtaaa gcctggcatg
     2461 acggccggaa ggagggcgag aaatttaacc gtcagctggc gctgacggga cattatgccg
     2521 gtgtcactgt cgggcagttg tggaaactca gccgggccat atccgggaat ggtatcacgc
     2581 aacatgcggc agccggtgcg ctggcacagg tggtgggcag tggggcattt catggaaacg
     2641 atattggtat ggtggcgaaa gctgccgcac agatggagcg atcggttggc cagtcggtca
     2701 gcgataccat aaatcagttt aagcggctga aggatgatcc tgtaaatgcc gcgaaggcgc
     2761 tggacaatgc gctgcatttt ttgaatgcca ctcagcttga gcagatacgc gtccttgggg
     2821 agcaggggcg gtccagtgat gcggcccgga tcgccatgtc tgcgctggca gaggaaaccg
     2881 gtaaacgcac atctgatatt gataataatc tcaatgcgct gggtagtacg ctgcaaacct
     2941 tgtctgactg gtggaagcag ttctgggatg cggccatgaa tattggtcgt gaagactcgc
     3001 tggatgcgca gattgatgcg ttacaggaaa aaattcagcg cgcgaaaaaa tatccgtgga
     3061 caaacgcctc cacacaggtg gagtacgatc agcagcgtct taacgatctt caggagaaaa
     3121 aacgccggaa ggatttgcag gatgcaaaag cgcaggcaga acggaattac caggagcaac
     3181 agaaacgccg gaatgctgaa aatgccgcgc tgaaccggat gaatgaaacg gaagctgcac
     3241 gacatcagcg ggaaattgcg cgtattaatg ccatgcagta cgccgaccag gctgtcaggg
     3301 atgcggcgat acagcgtgaa aatgaacgtt acgaaaaagc cattaagaaa aatacacggg
     3361 caacccgtaa tgatgaggcc acccggttat tgctgcagta cagtcagcag caggcacagg
     3421 tggaagggca gattgctgct gccagacagt cagcaggcat tgccactgaa aggatgactg
     3481 aagcgcataa acagcttctg gccctgcagc agcgcatcag cgacctggac gggaaaaagc
     3541 tgacggcaga tgaaaagagt gtgctggccc gtaagaatga gctgattcag gcgctgacgc
     3601 tgctggatgt gaaacagcag gagctgcaga aacagacagc gcttaacgac ctgagaaaaa
     3661 aaacggttca gctgaccagc cagctggcag acaaagaacg tgcactgcgt gagcagcaca
     3721 atctggatat tgccactgca ggtatggggg ataagcagcg gcagcgctac caggcacagt
     3781 tgcgcatccg gcaggaatac cggcaacagt tgcaacagct tgagaatgac agtcgccaga
     3841 aaggcactta cgggacggag gactaccgga gggctgagga ggtgctgaag gggagcctga
     3901 agcgacaact gaatgaaaac aaacgctact ggcaggaact ggaagtggcg cagggcgact
     3961 ggaaaaacgg tgccatgcgg gcgtttcaga attttacggc ggatgcggat aatgcggcgg
     4021 gaacggcaga acagatgttt acagtggcat tcagcagtgc cggtaatgca ctggcgacat
     4081 tctgtaccac cggtaagctg aattttaaat ccttcacctc ttccctgttg tcagatatgg
     4141 ccagaattat ggcacagatg gccatgatgc aggcggtaaa gggcgtcggt tctttattcg
     4201 gcttcacgac taatgctgat ggcggtgttt accagtctgc tgatttgagt cgctacagtg
     4261 gcacggtggt taaccgtccg acgttttttg cttttgcaaa aggcgcgggt gtgatggggg
     4321 aagcgggacc tgaagccatt ctgccactgc gtcgcggtgc tgatggtaag ctgggggttg
     4381 tggcggatac tggtggttca ggcatggtga tgtttgcccc gcagtacaac attgagatca
     4441 ataacgacgg cacgaacggg cagataggtc cggctgccct gaaggtggtt tatgacctcg
     4501 ggaaaaaagc ggccgcggac tttatgcaac agcaggcccg tgatggtggc cggttaagtg
     4561 gagcataccg gtaatggaga cgtttcactg gaaagtgcgc ccggatatga atgtggtatc
     4621 agagccgaaa gtggtgacag tgaagctggg cgatggttat gaacagcgtc gtgcggcggg
     4681 actgaataac cagttgtcga cttacagcgt gacgatacgt gttcgtaaag gtgaacaccc
     4741 atctttaaaa gcctttctgg aacggcacgg tggcgtccgc gcatttcagt ggacgccacc
     4801 ttatgactgg aaactgatca gggtggtttg tcgtaaatgg tcggcaagcg tgggggcgtt
     4861 atgggtgact gtaacggccg attttgaaca ggtggtgaac tgatgcagga tatccggcag
     4921 gaaacactga atgaatgcac ccgtgcggag cagtcagccc gggtggagct ctgggaaatc
     4981 gatctgacag aggtcggtgg tgagcgttat tttttctgta atgagcagaa cgaaaaaggt
     5041 gagccggtca cctggcaggg gcggcagtat caggcatacc ccattcaggg gtcggggttt
     5101 gaactgaacg gcaggggctg tgctgcccgt ccgacactga cggtctctaa cctgcacggc
     5161 atggtcaccg ggatggcgga agatctgcag agtctggtcg gcggaacggt ggtccggcgt
     5221 aaggtttacg cccgttttct ggatgcggtg aacttcgtca acggaaacag cgacgccgat
     5281 ccggaacagg aggtgatcag ccgctggcgc atcgagcagt gcagcgaact gagcgcggtc
     5341 agtgcctctt ttgtgctggc cacaccaacg gagacggatg gcgcggtttt cccggggcgt
     5401 atcatgctgg cgaatacctg tatgtggact taccgttctg atgagtgtgg ttacacgggc
     5461 agggcagtgg ctgacgagtt cgacaaacca acaacggata tccggaagga caaatgcagc
     5521 aagtgtatgc gcgggtgtga gttgcgcaac aataccggta atttcggcgg tttcctttcc
     5581 atcaataaac tttctcagta aatccatgac acagacagaa tcagcgattc tggcgcacgc
     5641 ccggcgatgt gcgccagcgg agtcgtgtgg cttcgtggtg agaacgccgg agggagacag
     5701 gtatcttccc agcgagaata tctccggtga gccggaggaa cggttccgga tggctccgga
     5761 ggactggctg cgggcacaaa tgcagggtga gattgtggca ctggtccaca gtcatcccgg
     5821 tggtctgccc tggctgagtg aggctgaccg gcggctgcag gtgcagagtg atttgccgtg
     5881 gtggctggtc tgccggggga cgattcacaa gttccgctgt gtgccgcatc tcaccgggcg
     5941 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc
     6001 ggggattgag atgccggatt ttcatcgcgg ggatgactgg tggcgtcacg gtcagaatct
     6061 ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcat cagcacaacc
     6121 gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg
     6181 cggcgatggt gagctgctgc accatattcc tgaacaactg agtaaacgag agaggtatac
     6241 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggaatggc acgcatctgc
     6301 ctttacgggg atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggggct
     6361 gaagccatcc gcgcactggc cacacagctc ccggcgtttc gtcagaaact gagtgacggc
     6421 tggtatcagg tgcgcattgc cgggcgtgat gcaggtgaaa ccgaattgtc tgcccgtctt
     6481 aatgagccgc tggcaaatgg tgcagtgatc cacatagtac cgcgtctggt gggagctaaa
     6541 agtggcggtg tgtttcaggc ggtgctgggg gcagctgtta tggcggttgc tatatggatg
     6601 ccgggggtag gaattatggc gagtaatctg ctgttttctc tcggtgccag tatgacgctt
     6661 ggcggtgttg cacagatgct ggcaccgaaa gccagaactc cccgtacaca gacaacggat
     6721 aacggcaaac agaacacctg tttctcctca ctggataaca tggttgccca gggcaatgtt
     6781 ctgcctgttc tgtacggtga aatgcgcgtg gggtcgcggg tggtatctca ggagatcagc
     6841 acggcagacg aaggggatgg tggtcaggtt gtggtgattg gtcgctgatg caaaatgttt
     6901 catgtgaaac cgcctgcggg cggttttgtc gtttatggag cgtgaggaat gggtaaaggc
     6961 agcagtaagg ggcatacccc gcgcgaagcg aaggacaacc tgaaatccac gcagttgctg
     7021 agtgtgattg atgccatcag cgaagggccg gttgacggtc cggtggatgg attaaaaagc
     7081 gtgctgctga acggtacgcc ggtcctggat agcgagggga agacaaactt ttccggtgtt
     7141 acggtggtgt tccgcgccgg cgagcaggag cagacaccgc cggaggggtt tgaatcttcc
     7201 ggctcagaga ctgtgctggg tacggaagtg aaatacgaca cgccgatcac ccggaccatc
     7261 acgtcggcaa acattgatcg tctgcgcctg accttcggtg tgcaggcact ggtggaaacc
     7321 acctcaaagg gggaccggaa tccgtcggaa gtccgcctgc tggttcagat acagcgtaac
     7381 ggtggctggg tgacggaaaa agacatcacc attaagggca aaaccacttc gcagtatctg
     7441 gcctcggtgg tggtgggtaa cctgccgccg cgcccgttta atatccggat gcgcaggatg
     7501 acaccggaca gcaccacaga ccagctgcag aacaaaacgc tctggtcgtc gtacaccgaa
     7561 atcatcgatg tgaaacagtg ctacccgaac acggcgctgg tcggcgtgca ggtggattca
     7621 gagcagttcg gtaaccagca ggtgagtcgc aattatcatc ttcgcgggcg cattctgcag
     7681 gtgccgtcga actataaccc gcagacgcgg caatacagcg gtatctggga cggaacgctt
     7741 aagccagcat acagcaacaa catggcctgg tgtctgtggg acatgctcac tcatccgcgc
     7801 tacggcatgg ggaaacgtct tggtgcggca gatgtggaca aatgggcgct gtatgtcatc
     7861 ggccagtact gcgaccagtc agtgccggac ggatttggcg gcacggagcc gcgcatcacc
     7921 tgtaacgctt acctgaccac acagcgtaag gcgtgggatg ttctcagtga tttctgctcg
     7981 gcgatgcgct gtatgccggt atggaacggg cagacgctga cgttcgtgca ggaccgaccg
     8041 tcggataaag tgtggaccta taaccgcagt aatgtggtga tgccggatga tggcgcgccg
     8101 ttccgctaca gcttcagcgc cctgaaggac cgccataatg tcgttgaggt gaactggatt
     8161 gatccggaca acggtcatga gacggcgaca gagcttgtgg aggacacgca ggccattgtc
     8221 cgttacggtc gtaacgtcac gaagatggat gcctttggct gtaccagccg gggacaggcg
     8281 caccgcgccg ggctgtggct gattaaaacg gaactgctgg aaacgcagac cgtggacttc
     8341 agcgtcggcg cagaagggct tcgccatgta ccgggtgatg tcattgaaat ctgcgatgat
     8401 gattatgccg gtatcagcac cggtggtcgc gtactggcgg tgaacagcca gacccggacg
     8461 ctgacgctcg accgtgaaat cacgctgccg tcctccggta ccacgctgat aagcctggtt
     8521 gacggaaatg gcaatccggt cagcgtggag gttcagtccg tcaccgacgg cgtgaaggtg
     8581 aaagtgagcc gggttcctga cggcgttgca gaatacagcg tgtgggggct gaagctgccg
     8641 acgctgcgcc agcgcctgtt ccgctgcgtg agtatccgtg agaacgacga cggcacgtat
     8701 gccatcaccg ccgtgcagca tgtgccggaa aaagaggcca tcgtggataa cggggcacac
     8761 tttgacggtg accggcgcgg cacggtgaat ggtgtcacgc cgccagcggt gcagcatctg
     8821 accgccgaag tcaccgcaga cagcggggaa tatcaggtgc tggcgcgatg ggacacgccg
     8881 aaggtggtga agggtgtgag cttcctgctt cgcctgaccg tggcagcgga cgacggcagt
     8941 gagcggctgg tcagcacagc caggacgacg gaaaccacat accgcttcag gcagctggcg
     9001 ctggggaatt acagtctgac agtccgggcg gtaaatgccc gggggcagca gggcgatccg
     9061 gcgtcggtat cgttccggat tgcggcaccg gcagcgcctg tcactattga actgataccg
     9121 gggtattttc agataacggt ggtcccgaaa ctggctgtat atgacccgac ggtgcagttt
     9181 gagttctggt tttcggaaaa gcggattgcg gatatcaggc aggttgaaac cagcgcccgc
     9241 tatcttggca cggcgctgta ctggatagcc gccagtatca atatcaggcc gggccatgat
     9301 tattattttt acgttcgcag tgtgaacacc gttggcaaat cggcatttgt ggaggccgtc
     9361 ggtcgggcga gcgatgatgc ggaaggttat ctgagttttt ataaagggtt gatcaataaa
     9421 acgcatctcg gcaaggagtt gtggacgcag attgataacg gtcagcttgc gccggacctg
     9481 actgaaatca ggacgtccat tacgaatgtc agcaatgaaa tcacgcaaac cgtcaataaa
     9541 aaactggaaa atcagagtgc tgcaatccag cagatacaga aagttcaggt tgatacaaat
     9601 aataacctga acagcatgtg ggctgtgaag ctgcaacaga tgaaggacgg acgcctttat
     9661 attgcgggta tcggtgccgg tattgagaat acgccagcag gaatgcagag tcaggtgctg
     9721 ctggcggcag acaggattgc gatgattaat cctgcgaatg gcaacacaaa gccgatgttt
     9781 gttggtcagg gcgatcagat attcatgaac gacgtgttcc tgaaacgcct gacggctccg
     9841 accattacca gcggcggtaa tcctccggca ttttccctga cacctggcgg acggctgacg
     9901 gcgaaaaatg ccgatatcag cggtaacgtg aacgcgaact ccgggacgct caacaacgtc
     9961 acgattaata agaactgtcg ggttctggga aaattgtccg cgaaccagat tgaaggcgat
    10021 ctcgttaaaa cagtgggcaa acctttccca cgggactccc gggcaccgga gaggtggcca
    10081 tcagggacca ttaccgtcag ggtttatgac gatcagccgt ttgatcggca aattgttatt
    10141 cccgcggtgg cgtttcgcgg tgctaaacat gagcggaaga ataacaatat ttattcgtca
    10201 tgccgcctga tagtgaagaa aaacggtgct gaaatttata accgaacgac cctggataat
    10261 acgctgatat atacgggtgt tattgatatg cctgccggtc acggtcacat gacgctggag
    10321 ttttctgtat cggcatggct ggtaaatggc tggtatccca cagcaagtat cagcgatttg
    10381 ctggttgttg tgatgaagaa atccacagca ggtatcagta tcagctgaat tttataaccc
    10441 agatacgggc accagaaatg gtgccttttt tattgcagaa aagcgagagg taattatgcg
    10501 taaactttat gctgccattt tgtccgcagc catctgtctg gctgtatccg gtgcgcctgc
    10561 atgggcgtct gaacatcagt ccacgctgag cgcggggtat cttcatgcct cgacgaacgc
    10621 tcccggcagt gatgatctga acgggattaa cgtgaaatac cgttatgagt ttacggacac
    10681 gctggggctg gtgacgtcat tcagctatgc caacgctgaa gatgagcaaa aaacgcatta
    10741 cagtgatacc cgctggcatg aggattccgt gcgtaaccgc tggttcagcg tgatggcggg
    10801 gctgtctgtg cgcgtgaatg aatggttcag cgcgtatgcg atggcgggcg tggcttacag
    10861 ccgtgtgtcg accttctccg gggattatct ccgcgtaact gacaacaagg ggaaaacgca
    10921 cgatgtgctg accgggagtg atgacaaccg ccacagcaac acgtctctgg cgtggggggc
    10981 tggcgtgcag tttaacccga ccgaatccgt ggccattgac cttgcttatg aaggttccgg
    11041 cagtggcgac tggcgcactg acggtttcat cgtgggtgtc ggttataagt tctgattagc
    11101 caggtaacac agtgttatga cagcccgctg gttcaggcgg gctttttgtg gggtgaatat
    11161 ggcagtaaag atttcaggtg tactgaaaga tggggcgggt aaacctgtcg taaattgtgc
    11221 gattgaactg cgggccagaa gaaccagtcc gaccgttgtg gcacacgttg ttgccacttg
    11281 cgtgacggac aataacggtg cttatgtgat tgaggctgag ccggggtatt acgaggttgc
    11341 gcttcactgt aacggctggc agccaacccg tgtcggggat attgatgtgg caccgactga
    11401 tgcaccgggg acactgaacg cgtttctgaa tgcaccaaag gatggtgatt tacgtccgga
    11461 ggtgatgaag cgctttgagg aaatggtggc gcaggcgcag cagagtgccg gggc
//
LOCUS       DAJTUI010000110        11307 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTUI010000110 DAJTUI010000000
VERSION     DAJTUI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.110"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(88..363)
                     /gene="yjfN"
                     /locus_tag="OO464_003347"
     CDS             complement(88..363)
                     /gene="yjfN"
                     /locus_tag="OO464_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6228382.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(512..841)
                     /gene="bsmA"
                     /locus_tag="OO464_003348"
     CDS             complement(512..841)
                     /gene="bsmA"
                     /locus_tag="OO464_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6228383.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1023..1772
                     /gene="yjfP"
                     /locus_tag="OO464_003349"
     CDS             1023..1772
                     /gene="yjfP"
                     /locus_tag="OO464_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6228384.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(1769..2524)
                     /gene="ulaR"
                     /locus_tag="OO464_003350"
     CDS             complement(1769..2524)
                     /gene="ulaR"
                     /locus_tag="OO464_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6228385.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2632..3696)
                     /gene="ulaG"
                     /locus_tag="OO464_003351"
     CDS             complement(2632..3696)
                     /gene="ulaG"
                     /locus_tag="OO464_003351"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6228386.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPSTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4051..5448
                     /gene="ulaA"
                     /locus_tag="OO464_003352"
     CDS             4051..5448
                     /gene="ulaA"
                     /locus_tag="OO464_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR6228387.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5464..5769
                     /gene="ulaB"
                     /locus_tag="OO464_003353"
     CDS             5464..5769
                     /gene="ulaB"
                     /locus_tag="OO464_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6228388.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            5779..6243
                     /gene="ulaC"
                     /locus_tag="OO464_003354"
     CDS             5779..6243
                     /gene="ulaC"
                     /locus_tag="OO464_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR6228389.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6257..6907
                     /gene="ulaD"
                     /locus_tag="OO464_003355"
     CDS             6257..6907
                     /gene="ulaD"
                     /locus_tag="OO464_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6228390.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            6917..7771
                     /gene="ulaE"
                     /locus_tag="OO464_003356"
     CDS             6917..7771
                     /gene="ulaE"
                     /locus_tag="OO464_003356"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR6228391.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            7771..8457
                     /gene="ulaF"
                     /locus_tag="OO464_003357"
     CDS             7771..8457
                     /gene="ulaF"
                     /locus_tag="OO464_003357"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6228392.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8586..8861)
                     /locus_tag="OO464_003358"
     CDS             complement(8586..8861)
                     /locus_tag="OO464_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6228393.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9188..9583
                     /gene="rpsF"
                     /locus_tag="OO464_003359"
     CDS             9188..9583
                     /gene="rpsF"
                     /locus_tag="OO464_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6228394.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9590..9904
                     /gene="priB"
                     /locus_tag="OO464_003360"
     CDS             9590..9904
                     /gene="priB"
                     /locus_tag="OO464_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6228395.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            9909..10136
                     /gene="rpsR"
                     /locus_tag="OO464_003361"
     CDS             9909..10136
                     /gene="rpsR"
                     /locus_tag="OO464_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6228396.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10178..10627
                     /gene="rplI"
                     /locus_tag="OO464_003362"
     CDS             10178..10627
                     /gene="rplI"
                     /locus_tag="OO464_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6228397.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(10698..11261)
                     /locus_tag="OO464_003363"
                     /pseudo
     CDS             complement(10698..11261)
                     /locus_tag="OO464_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2688 a         2795 c         2962 g         2862 t
ORIGIN      
        1 gattaactaa atgaaatatg tgaaaattgt aggccggaca aggcgctcgc gccgcatccg
       61 gcattgttca tttgctgtag atgacactca tgcatacagt atcgcctgta cgcgccagtt
      121 atcaggctgc tggtcttcgt acatggtaat aatgcggtac catgacgcac cttgttcatc
      181 ggcctttaac gccacgatac gttccacgtc ctgcggatcc cctgaaatat tgctaactga
      241 gatctgacct atttcattca ggcccgtcac gcagtcagca ctggcgaatt ctgcagactg
      301 tgccgtggcg ctcgcaggat aagtcactaa ttgcagcgag ggtgaggcaa gtaattgttt
      361 catcgtcagc tccatttttc gtttccccac atgttgcagg gcattccatc gcggtaataa
      421 acgcccaagc ttccagccgg taatttattg tgcacaggta aacgcatagg gacgcaaagc
      481 aatgtaaagc tgactatgta agttgtctga tttatttacg gtacaaaatg gcttgtgaat
      541 accactgtcc tgtcacgatg gtttcgtcca ccatgacgac aacgtaataa tcagcttttg
      601 cagcgacagc ttttgctctg atttctgcta atgcgtcatc cggagaaccc cgaaccatcg
      661 tgcttacgct acctattcgc tgtaaccctt gcgtctggtc gcggcgaatc tcttgcggat
      721 ggtccgttac tggcggtgct ggctgtggcg taccttgcag tgcgctacag gcacttaaca
      781 tcaacaccaa taataaactg gcaaaccggt aaataacgct attacgtttc ctgctaacca
      841 tagtgtagtg ccttattaat ttaactttgg gggaatgttc ccgaattgtt acctggtacg
      901 cgattttcga atgaaaacgt gacgaaagcg taacccacat ctcaacatta tgaactaata
      961 ccgccagcag aaacttgtgc taaggatttc ttcatgtcat accagacaaa aaggagagac
     1021 agatgattga aatagaatca cgcgagctgg cagatattcc cgttcttcat gcttatcctg
     1081 tcgggcaaaa agataccccg ttaccgtgcg taatttttta tcacggcttt acttcatcca
     1141 gcctggtgta tagctatttt gccgttgcgc tggcgcaggc tggtttgcgg gtgatcatgc
     1201 cggatgcgcc cgatcacggt agccgtttta gtggcgacga agcgcggcgg ttaaatcaat
     1261 tctggcaaat cttgctacaa agtatgcagg aattcactac tttacgtgcg gcaatagccg
     1321 aagaaaactg gctgcttgat gaccgtctgg cagtcggtgg cgcgtcgatg ggcgcgatga
     1381 cggcactggg aattaccgct cgccacccca cggtgaaatg taccgccagc atgatgggat
     1441 cgggctattt tacatcgctc gcccgttcac tgtttccacc gctgatacct gaaacggcag
     1501 cacagcagaa tgaattcaat aaaattgtcg cgccgctggc agagtgggaa gcgacaaacc
     1561 acctggaaca acttggggac agacctctac tgctgtggca tggcctcgac gatgacgttg
     1621 tgcctgccga cgaatcacta cgtttgcagc aggccttaag cgagacggga cgggataaac
     1681 tgctaacctg ttcatggcag ccaggcgtgc gtcaccgcat tacgcctgag gcgttagatg
     1741 ctgccgtgac atttttccgc cagcatcttt aaacacgcag aatgctgacc ccttgcgctt
     1801 ccagttgttg caggatttcc gggttagcat ttttgccggt gatcagcata tcgatttgat
     1861 cggcacggct aaaaagcatt cccgcgcgtt cgccaatctt actgctatca accagtacca
     1921 ccagtttccc taccacgctc agcatcttct gctctgccat tgctgtcagc atatcggttt
     1981 tatacaaccc ttctgcggtc agcccttttc cgctggtaaa catccagtgc ccggcataga
     2041 gactgttttc gctgccctgc gggcttaaag tgatggactg acttttgttg tactgtccac
     2101 ccataatgat cacgctgtca tgttcttgat cgatcaggta atttgccagc ggtagataat
     2161 tagtgatgat ttgcactggc ttgccacaca tttcccgccc aagcagaaac gcggtggagc
     2221 cgcagttgat gactacgctt tcgcccggat taaccagctg cgaggccgct ttagcaatac
     2281 gtactttttc atcgtgattc tgcgcctgat gcagattcat cggcgtccag cgcgggcgct
     2341 gttgggtaat agcttctgcg ccattgcgca cctttttcag tttgccgctt tcgccaagtt
     2401 tattgatatc gcgtcgcgca gtggcaggcg aaattcccag acgctcaacg actttctcaa
     2461 cggtcacaaa gcccaattgt gcgagcattt ccaggaggat ttgatgtctt tgtgcttcag
     2521 tcatgagcta ttccgataaa atttgatttt tttagatgat atttgaaata gccaggaaat
     2581 actacgccgg atagcgcgaa cttctccacg ctatccggta taaacgacag attacaggaa
     2641 tgacttgaac ggcagatccg gttcaatagt gaagcaatca tcgaaaccgc gcggatagtg
     2701 gtactcgaag ttgtctttat ccagcggcca ggtaaatttg ccgccaacct gccagataaa
     2761 cggcttgaag ccatacttca gacgatcttt tttgatctcc cacagcacgc ggatctcttg
     2821 cggatcggcc tggaagtttg accagatgtc gtggtggaac gggataacca ctttcgcatt
     2881 cagggcttca cccatacgca gcatatcggc gctggtcatt ttgtcggtaa taccgcgcgg
     2941 gttctcacca tacgatccta acgccacatc gatctgatgt tcgttaccgt gcttcgcata
     3001 atagttagag tagtgggaat cgccactgtg atatagggaa ccgccagggg ttttgaacag
     3061 gtagtttact gcgcgatcgt ccatgccatc tggcagtacg ccagccgctt tttgatcggc
     3121 aggcagggtg atcagtgcag tacggtcgaa agcgtcaagc gcatgaattt caatgtcttt
     3181 cacttttaca acatcgcccg gtttgaccac gatgcaacgc tctttcggta cgccccagcc
     3241 aatccacaaa tcaacgcagg ttttcggtcc aatgaatggt acgtcatcag cacagttctg
     3301 catcacggca gcagcgacgt taacgtcgat atgatcgttg tgatcgtgag tcgccagtac
     3361 cgcgtcgatc tggcgaatcg caaacggatc aagaacaaac ggggtggtgc gtaggtttgg
     3421 ctgcagtttt ttcacgccag ccatgcgctg catctggtga ccctgtttca ttaacgggtt
     3481 accgtgactt tgtttgccag tgccgcacca gaaatcaacg caaacgttgg tgccaccttc
     3541 tgatttcaac cagatcccgg tgcagcctag ccaccacatc gcaaatgtgc taggggcgac
     3601 ctgctcttgt tcaatttctt cattcagcca gctaccccac tccgggaaag tgctcaggat
     3661 ccaggattct cgggtgatac ttttcacttt actcatcgcc atttaccttc atgatagttc
     3721 aattcgaatc aacatgtgat tggttttgat taatcctgac actattcctt caggaaggta
     3781 atgaccattt tttgactttt tgccagggaa gttgttgttg attttgagtg gggaaagatt
     3841 taatggaatg tgtagttaaa ttaactaaat gaatttaaat aaataattgt ttcgttgtgt
     3901 gaatcccact ctatccatgt ggaattattt gcgggtcgcg tcacatttaa tcataaataa
     3961 tcttgttgtg attacttttg aaaattagag tgagtgcaca acattccggg tgtgcggaat
     4021 acccggttac ctcttcttca ggagatcgtt atggagatcc tctacaacat ctttaccgtg
     4081 ttttttaacc aggtcatgac caatgccccg ttgttgctgg gtattgtgac ctgtctgggc
     4141 tacatcctac tgcgcaaaag tgtcagcgtt attattaaag gcacgattaa aaccataatt
     4201 ggtttcatgt tgttgcaggc agggtccggc atcctcacca gcaccttcaa accggtggtg
     4261 gcgaaaatgt ccgaagtcta cggcattaac ggcgcaattt ccgatactta cgcgtcgatg
     4321 atggcaacca tcgaccgcat gggcgatgcc tatagctggg taggttacgc ggtgttgtta
     4381 gcgctggcgc tgaacatctg ttacgtgctg ctgcgtcgca ttaccggtat tcgtaccatc
     4441 atgctgactg gtcatatcat gttccagcag gcggggctga ttgccgttac gttgtttatt
     4501 ttcggctact ccatgtggac aaccattatc tgcacggcga ttctggtttc gctctactgg
     4561 ggcattacct ccaacatgat gtacaagccg actcaggaag tgacggacgg ctgcggtttc
     4621 tccatcggtc accagcaaca gtttgcatca ttaattgcct ataaagttgc gccgttcctc
     4681 ggcaaaaaag aggaaagcgt tgaagacctc aaactgccag gctggctgaa tattttccac
     4741 gacaacatcg tctccacggc gattgtcatg accatcttct ttggtgccat tctgctctcc
     4801 ttcggtatcg acaccgtgca ggcgatggca ggcaaagtgc actggacggt ctatatcctg
     4861 caaaccggtt tttccttcgc ggtggcgatc ttcatcatca ctcagggtgt gcgcatgttt
     4921 gtggcggaac tctctgaagc gtttaacggt atctcccagc gcctgatccc tggtgcggtt
     4981 ctggcgattg actgtgcggc tatttatagc ttcgctccga acgccgtggt ctggggcttt
     5041 atgtggggca ccatcggtca gctgattgcg gttggcatcc tggtcgcctg cggttcctcg
     5101 atcctgatta tcccaggctt tatcccgatg ttcttctcca acgccaccat cggcgtgttc
     5161 gctaaccact tcggcggctg gcgtgcggcg ctgaagattt gtctggtgat ggggatgatc
     5221 gaaatctttg gttgcgtctg ggtggtgaaa cttaccggta tgagtgcctg gatggggatg
     5281 gcggactggt cgattctggc accgccgatg atgcagggct tcttctccat cggtatcgcc
     5341 tttatggccg tcatcattgt aattgcactg gcttatatgt tcttcgctgg ccgcgcactg
     5401 cgcgcagaag aagatgcaga aaaacaattg gcagaacagt ctgcttaata aggagttttg
     5461 attatgaccg tacgtattct ggctgtgtgt ggcaacggac aaggcagttc catgatcatg
     5521 aagatgaaag tggaccagtt tttaacccaa tcaaacattg accacacggt aaatagctgc
     5581 gcggttggcg aatacaaaag cgagttgagc ggcgcggata tcatcatcgc ttctacgcac
     5641 attgcgggcg aaatcaccgt gaccggcaac aaatacgtgg ttggcgtgcg caacatgctc
     5701 tctcctgccg actttggccc gaaactgctg gaagtgatca aagcgcattt cccgcaggat
     5761 gtgaagtaag gacgcgccat gaaattacat gattcgctgg cggagaataa atccatccgc
     5821 ctgcaggctg aagctgagac atggcaggac gcagtaaaaa tcggcgttga cctgctggtg
     5881 gcggcggatg ttgtcgagcc gcgttactac caggcgattc tggatgcggt tgaacagcac
     5941 ggtccgtact ttgtgctcgc accaggcctg gcaatgccgc acggtcgccc ggaagagggc
     6001 gttaagaaaa ccggttttgc gctggtgacg ctgaaaaagc cgctggagtt caaccacgaa
     6061 gataacgacc cggtggatat cctcatcacc atggcggcgg tcgatgccaa cacgcaccag
     6121 gaagtgggca tcatgcagat cgtcaacctg tttgaagatg aagaaaattt cgaccgttta
     6181 cgtgcctgcc gtaccgagca ggaagtactg gatctcattg accgcaccaa cgcggcagct
     6241 taagaaggaa ttgaagatgt cattaccgat gttgcaagtc gcgctggaca accagactat
     6301 ggatagcgcc tacgaaacca ctcgcctgat tgccgaagaa gtcgacatta tcgaagtggg
     6361 taccattctg tgcgtgggcg aaggcgtgcg tgcggttcgc gatctgaaag cgctctaccc
     6421 gcacaaaatc gtgctggcag acgccaaaat tgccgatgcg ggcaaaattc tttctcgtat
     6481 gtgcttcgaa gccaacgctg actgggtgac cgtgatttgt tgtgcggata tcaacaccgc
     6541 caaaggtgcg ctggacgtag caaaagagtt taacggcgac gtgcagatcg aactgaccgg
     6601 ttactggacc tgggaacagg cgcaacagtg gcgcgacgca ggtattcagc aggtggttta
     6661 tcaccgcagc cgtgacgcgc aggccgctgg cgtggcgtgg ggtgaagccg atatcactgc
     6721 gatcaaacgt ctttccgata tgggcttcaa agtcaccgtc accggtggcc tggcgctgga
     6781 agatctgccg ctgttcaaag gcattccaat tcatgtgttt atcgccggtc gcagcatccg
     6841 tgatgccgct tctccggttg aagccgcgcg tcagttcaaa cgttccatcg ccgaactgtg
     6901 gggctaagga gcggatatgt tgtccaaaca aatcccgctt ggcatctatg aaaaagcgct
     6961 ccccgccggg gagtgctggc tggaacgcct gcgactggca aaaacgttgg gcttcgattt
     7021 tgtcgagatg tcggtagatg aaactaacga gcgcctgtcg cgccttgact ggagccgcga
     7081 acagcgtctg gcgctggtga atgctattgt tgaaaccggc gtgcgtgtgc cgtccatgtg
     7141 cctttctgcc catcgtcgtt tcccgttggg cagcgaagat gacgcggtgc gggcgcaggg
     7201 gctggagatt atgcgtaaag ccatccagtt cgcccaggac gttggcattc gcgtgatcca
     7261 gctggcgggc tatgacgttt actatcagga agccaataac gaaacgcgtc gtcgtttccg
     7321 tgacggcctg aaagagagcg ttgagatggc gagccgcgcg caggtgacgc tggcgatgga
     7381 gatcatggat tatccgttga tgaactccat cagcaaggcg ctgggttacg cgcactatct
     7441 caacaacccg tggttccagc tctacccgga tatcggcaac ctgtcggcgt gggacaacga
     7501 tgtgcagatg gagttgcagg ccggaatcgg gcatatcgtc gcggtgcatg tgaaagacac
     7561 caaaccgggc gtcttcaaaa acgtgccatt tggcgaaggt gtggtggatt tcgaacgttg
     7621 cttcgaaacg ctcaaacaga gtggctattg cgggccgtac ctgattgaga tgtggagcga
     7681 aacggcggaa gacccggcgg cagaagtggt gaaagcgtgt gattgggtga aagcgcgcat
     7741 ggccaaagca ggcatggtgg aggcggcata atgcaaaagc taaaacagca ggtgtttgaa
     7801 gccaacatgg atctgccgcg ctatgggctg gtgaccttta cttggggcaa cgtcagcgct
     7861 atcgaccgcg aacgcgggct ggtggtgatc aagcccagcg gcgttgccta cgaaaccatg
     7921 aaagcagacg atatggtggt ggtcgatatg agcggcaagg tggtggaagg ggagtatcgc
     7981 ccatcttccg acactgcgac gcatctcgaa ctctaccgtc gttacccgtc gcttggcggc
     8041 attgtccata cccactccac tcatgccacc gcatgggcgc aggcggggct ggcgatcccg
     8101 gcattaggca ccacgcacgc tgactacttc tttggtgata tcccgtgtac gcgtggatta
     8161 agcgaagaag aggtgcaggg tgagtatgaa ctgaacactg gcaaagtgat tatcgaaacg
     8221 ctgggcaacg ccgagccgct gcatacgccg ggaattgtgg tgtatcagca cggaccgttc
     8281 gcctggggga aagatgccca cgacgcggtg cataacgcgg tagtgatgga agaagtggcg
     8341 aaaatggcgt ggattgcgcg cagtattaat ccacaactca atcatatcga cagctacctg
     8401 atgaataaac acttcatgcg taagcacggt cctaacgctt attacgggca gaagtaaaac
     8461 acgcgctgcg gaaatttcct tcctcgggag ataactggtc taattccgca gccgtttttc
     8521 aaaaaaagcc ccctgcgaag ggggcaaagc aaactatggc aatgtttcgt tggttatacc
     8581 tggtgctagc gataaatatc cgcgctggcg tgcatattgc cgttactccc cggttcccgc
     8641 atcagaatta cgtggtagta cgttgcgcct tgcgcatctg tttcttcatt taatgcctga
     8701 cgtgcttcgc tttcagtggc gaaattatga ttgatataaa tcacgcctaa gctttgcaca
     8761 tcgtccatat ttctggcctg gtggttatta atttcaatgg ctgcccatgt atttgcactt
     8821 agcaaaagca cagccagaag ggctaaaact cgactgaaca tagatacctc ctcgacggct
     8881 gactttgtgt gctctcctct gtgatgatct tctgatttaa ttttaatcaa tgataaagaa
     8941 gttgatggtg accatttctg atgcagttgt tcaaaaaaac actgtgacgg aatgtgatga
     9001 acttcaaatc agcggtttag aggttatttg cgaaagggga gatttatttc tgctccgccc
     9061 ttgagtttag cggggtatgc cagtactata acgcgtcatt tttcagccga cctttaacac
     9121 gttccttgcc tccccgggat tcggctgacc cagacaggag gctgaataat ccgtaaggag
     9181 caattcgatg cgtcattacg aaatcgtttt tatggtccat cctgaccaga gcgaacaggt
     9241 tccgggcatg atcgagcgct acactgctgc catcactggt gcagaaggca agatccaccg
     9301 tctggaagac tggggccgcc gtcagctggc ttacccgatc aacaaactgc acaaagcaca
     9361 ctacgttctg atgaacgttg aagctccgca ggaagtgatc gatgagctgg aaactacctt
     9421 ccgcttcaac gatgccgtta tccgcagcat ggttatgcgt accaagcacg ctgttaccga
     9481 agcatctccg atggttaaag cgaaagacga gcgccgtgag cgtcgcgatg atttcgcaaa
     9541 cgaaaccgct gatgatgctg aagctgggga ttctgaagag taatttctga tgaccaaccg
     9601 tctggtgttg tccggcaccg tgtgcaggac tccccttcga aaggtcagtc catcaggaat
     9661 tcctcactgc cagttcgtgc ttgagcatcg ttctgtgcag gaggaagccg gctttcaccg
     9721 gcaggcatgg tgtcaaatgc ccgttattgt tagcggacac gaaaaccagg ccattactca
     9781 cagtataacg gtcggcagtc gcataaccgt tcaggggttc atttcatgcc acaaggcaaa
     9841 gaacggactg agcaaaatgg ttttgcatgc cgagcagatt gaattgatag attctggaga
     9901 ctagccatat ggcacgttat ttccgtcgtc gcaagttctg ccgtttcacc gcggaaggcg
     9961 ttcaagagat cgactataaa gatatcgcta cgctgaaaaa ctacatcacc gaaagcggta
    10021 agattgtccc aagccgtatc accggtaccc gtgcaaaata ccagcgtcag ctggctcgcg
    10081 ctatcaaacg cgctcgctac ctgtccctgc tgccgtacac tgatcgccat cagtaatcgg
    10141 tcacggtcca ttaatacgac tttgagagga taaggtaatg caagttattc tgcttgataa
    10201 agtagcaaac ctgggtagcc tgggtgatca ggtaaacgtt aaagcgggct atgctcgtaa
    10261 cttcctggta ccgcagggta aagctgttcc agctaccaag aaaaacattg aattcttcga
    10321 agcacgtcgc gctgaactgg aagctaaact ggctgaagtt ctggcagctg ctaatgctcg
    10381 cgctgagaaa atcaatgcac tggaaactgt taccatcgcg tctaaagctg gcgacgaagg
    10441 taaactgttc ggttccatcg gtactcgcga catcgctgac gctgtaactg cagctggcgt
    10501 tgaagtggct aagagcgaag ttcgtctgcc gaacggcgtt ctgcgtacca ctggcgaaca
    10561 cgaagtgagc ttccaggttc acagcgaagt attcgcgaaa gtgatcgtaa acgtagtagc
    10621 tgaataattc gttattcaac gagacgtaaa aacgccgacc attggtcggc gttttgcttt
    10681 ctatttttcg tcaggcatta atttcgcaag tagatccatt caccattctc ttgtgtccat
    10741 aagtcaggat ggtttgctgg cgttagatgc atacgatcac tccttatgtc tcggtcctgc
    10801 ggcactcgct cgaaacctaa acccgagtag attgcggctg aatttgataa ggaacgtaga
    10861 ccaattttca aatcatgggg taaatcagaa tacgcgttta gcaatgcagc aatgatgaat
    10921 ccgagggcat tagaaacgcc tgtaacagtg tgaagttggt cgatataaag atcgattgtt
    10981 ggtagttcag atgagcagca attaggcatt tgggcaggaa gggttacaac gtatcctatc
    11041 gtttggccgg tatagtctgg agtactataa ccaggtccca aaacaacctc gtgtattgtg
    11101 tatttactgt tgggttcgaa ggattcgaca tcttgcaata aatatttcag ttctgcggtt
    11161 aacctcgcac ccgcgtaagc tttattgtat ttctgggcgt gaatattatc taatgtagct
    11221 tcatggttcc atccaaaaga attatcacca ttcattgtta ctgaatcgcc acgggtttaa
    11281 cagacacctc agagtcattt aagatgg
//
LOCUS       DAJTUI010000111        11215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTUI010000111 DAJTUI010000000
VERSION     DAJTUI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.111"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            587..784
                     /locus_tag="OO464_003364"
     CDS             587..784
                     /locus_tag="OO464_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228398.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(734..1927)
                     /locus_tag="OO464_003365"
     CDS             complement(734..1927)
                     /locus_tag="OO464_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6228399.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2063..3787
                     /gene="iucA"
                     /locus_tag="OO464_003366"
     CDS             2063..3787
                     /gene="iucA"
                     /locus_tag="OO464_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6228400.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3788..4735
                     /gene="iucB"
                     /locus_tag="OO464_003367"
     CDS             3788..4735
                     /gene="iucB"
                     /locus_tag="OO464_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6228401.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4735..6477
                     /gene="iucC"
                     /locus_tag="OO464_003368"
     CDS             4735..6477
                     /gene="iucC"
                     /locus_tag="OO464_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6228402.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6474..7811
                     /gene="iucD"
                     /locus_tag="OO464_003369"
     CDS             6474..7811
                     /gene="iucD"
                     /locus_tag="OO464_003369"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6228403.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7817..10012
                     /gene="iutA"
                     /locus_tag="OO464_003370"
     CDS             7817..10012
                     /gene="iutA"
                     /locus_tag="OO464_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6228404.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10563..10706)
                     /locus_tag="OO464_003371"
     CDS             complement(10563..10706)
                     /locus_tag="OO464_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228405.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10945..>11215
                     /locus_tag="OO464_003372"
     CDS             10945..>11215
                     /locus_tag="OO464_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HCR6228406.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFS"
BASE COUNT         2822 a         2806 c         2897 g         2690 t
ORIGIN      
        1 aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa tcatgactcc
       61 tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag ttttacagga
      121 tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc ccaaaaaaca
      181 taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt caggtgtttg
      241 cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca gtaaaaatac
      301 ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa aagaaacctg
      361 gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc ctgataccca
      421 tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac gcgcagcgca
      481 gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca gagcaacgct
      541 gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg agattacacc
      601 gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca gaacactgcc
      661 tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact ccagtaaggt
      721 gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac acaatggtaa
      781 ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct gttttcgata
      841 ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta gcgagccagc
      901 ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg ggtgttgcca
      961 gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc tgttcggaga
     1021 gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg gccaccggag
     1081 tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact gcggacagac
     1141 ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac tggcgagtaa
     1201 gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat attgcgcaaa
     1261 gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt gtgggctgtg
     1321 gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct gccatcagca
     1381 gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc ggcccgaata
     1441 gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga ttcccttctc
     1501 cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg gtcagcccgt
     1561 aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg gcgctggtag
     1621 ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct cccagagccc
     1681 acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac ggggacgatg
     1741 gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga ctcagatttg
     1801 tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata ccgagaagcc
     1861 cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct tcgatccctg
     1921 gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa attaatttat
     1981 ctgacaataa catttattgt tgataatgag aatcattatt gacataattg ttattatttt
     2041 actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca cagatgtggc
     2101 tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac tgacagaata
     2161 cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt tcagagtggc
     2221 ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg tcacggcatc
     2281 aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata agctacgcca
     2341 tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc tggaaagcca
     2401 tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc gtgaaaaagc
     2461 gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc accctgcgcc
     2521 taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg atatggcacc
     2581 ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg aaagtttgca
     2641 tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc agttactcaa
     2701 cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg aatatctttt
     2761 gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact taggtgaggc
     2821 cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca ccagccgcga
     2881 tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc tgtccgtgaa
     2941 agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct ggcagatgct
     3001 acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg ggctgctcga
     3061 tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc tgctggtgga
     3121 tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac ctgatggcgg
     3181 tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta tcactgtgca
     3241 acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc cgctgtttac
     3301 ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc ttgtccagat
     3361 gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg cttttatgcc
     3421 tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata ttttcaccca
     3481 tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt ttgctgtgac
     3541 cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc gtgtacgagc
     3601 atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact acgtgctgga
     3661 aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc ataacgagaa
     3721 caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc aggctcagga
     3781 ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct gtgaaaaact
     3841 cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc actgtcccgg
     3901 ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg ccgcaccaca
     3961 actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg cgctgacgct
     4021 ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt taccgttatg
     4081 gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag agcgtgagat
     4141 ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt acgatccacg
     4201 tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag aacgtttcac
     4261 ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct cactggaggt
     4321 acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc tgattgggtg
     4381 cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag accgcattgg
     4441 tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg gtgaacagca
     4501 atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt acctgctgct
     4561 ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc agcgcctgtt
     4621 ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc cacataagcg
     4681 ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc tgtaatgaat
     4741 cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc tgagatggag
     4801 tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat taacctgccg
     4861 ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg gattgatgcc
     4921 caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat gcagctaaag
     4981 ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt gtatgccacg
     5041 ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag tgacctgatt
     5101 aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt cgtttttaat
     5161 aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga gtataccaac
     5221 accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg ctgcgataac
     5281 gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt tacacgcttc
     5341 agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc ggtacatccg
     5401 tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga aggcaggatg
     5461 gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg taccctgacc
     5521 aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta caatacctca
     5581 tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc acgctggctg
     5641 caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat ccttggtgaa
     5701 ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc tccctatcgc
     5761 taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct gaaaccggat
     5821 gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca gccgctggca
     5881 ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca attgttccgg
     5941 gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct tattgcacac
     6001 ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct gctgaaagac
     6061 ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc tttgcctcag
     6121 gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga tttgcagacg
     6181 ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct tggcgtacct
     6241 gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa caaacatcca
     6301 caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat tcgcgtggtg
     6361 ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat gctgccgaat
     6421 taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga atcatgaaaa
     6481 aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt gctgcattgt
     6541 cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat ttttcctggc
     6601 atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa gatctggtca
     6661 gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag cacaaaaagt
     6721 tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt tctgactatc
     6781 tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt gaaaatattg
     6841 atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa tattttgccc
     6901 acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg aagcatgtga
     6961 cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg agtggaaaac
     7021 ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat gcattacgcg
     7081 gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat tttaacgcac
     7141 tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca ggcttctccg
     7201 gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca tcggatggca
     7261 tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt gaagttctga
     7321 gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg gaaagtagtg
     7381 gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag agcctggaaa
     7441 gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata cttccctcac
     7501 tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat gacttcactc
     7561 tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc atgcaaactc
     7621 atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt attcttaatc
     7681 gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt cagtggcgca
     7741 gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat acagcaaata
     7801 ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc cgctgcttct
     7861 tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca tagtgtctgc
     7921 cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta tcgaaaatgc
     7981 cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg ctcagttaat
     8041 ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca tgcgtggccg
     8101 cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg acagccgaca
     8161 actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg gcgcgacggc
     8221 cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa aaggtcagcc
     8281 ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca gtaaagatca
     8341 cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg gacgtctttc
     8401 cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca ccctgcttga
     8461 taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa ccggtacgct
     8521 gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa gtcagggaga
     8581 cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga gcagcacacc
     8641 atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc atttgatcag
     8701 cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg tttactaccg
     8761 cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac aggcgacggc
     8821 tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc tgaacagcca
     8881 actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc gctttacctc
     8941 caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga acaaccataa
     9001 gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg ccttcctgca
     9061 atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct atcagtatac
     9121 tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg ctaccgggaa
     9181 ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact ttctgttcaa
     9241 tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt tttcccaggg
     9301 ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg cagcagtgaa
     9361 cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg aaggcgtgaa
     9421 agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga ctcaaatcgc
     9481 ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga ccatcagtgt
     9541 gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc tgatcccgga
     9601 tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga aagtgaacgg
     9661 tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag cttacattaa
     9721 ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg acgtaagcga
     9781 tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta gttggcagct
     9841 tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact ataccactgt
     9901 ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt cactgtacga
     9961 ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct gacggggatg
    10021 cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt tgtttacagc
    10081 cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt tcaccactga
    10141 ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc ctcatttcat
    10201 tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct ggcagatcct
    10261 gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg ctgcaatgaa
    10321 ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa aaagaggtac
    10381 ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct gtcataaagt
    10441 ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc cggcccggag
    10501 gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg ctataggcaa
    10561 agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt ctgaataacg
    10621 cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat cgctaattcg
    10681 tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg aaatattaaa
    10741 cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag tattgattca
    10801 cataatctct catcattaat gagaacattg tcacatattt acaatataga actgttactt
    10861 ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata ctttgttcat
    10921 tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata tagtgctgcc
    10981 actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa aacaaaccga
    11041 aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc agcaaatatt
    11101 gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa aggtattttc
    11161 cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt ctctt
//
LOCUS       DAJTUI010000112        11160 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTUI010000112 DAJTUI010000000
VERSION     DAJTUI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.112"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="OO464_003373"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO464_003373"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO464_003374"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO464_003374"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR6228407.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO464_003375"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO464_003375"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6228408.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO464_003376"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO464_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO464_003377"
     CDS             complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO464_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6228409.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6641
                     /gene="garD"
                     /locus_tag="OO464_003378"
     CDS             5070..6641
                     /gene="garD"
                     /locus_tag="OO464_003378"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR6228410.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO464_003379"
     CDS             complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO464_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR6228411.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO464_003380"
     CDS             7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO464_003380"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR6228412.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9069..9542
                     /gene="agaV"
                     /locus_tag="OO464_003381"
     CDS             9069..9542
                     /gene="agaV"
                     /locus_tag="OO464_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR6228413.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9553..10332
                     /locus_tag="OO464_003382"
     CDS             9553..10332
                     /locus_tag="OO464_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR6228414.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10322..11131
                     /locus_tag="OO464_003383"
     CDS             10322..11131
                     /locus_tag="OO464_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR6228415.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
BASE COUNT         2670 a         3034 c         2802 g         2654 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccaccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacacc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaagg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccaag ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatcccta aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact
     5521 atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5581 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca gcagttgtac
     5641 ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga
     5701 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5761 aagctattcc agtagaaagc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5821 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5881 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat tgcggtggca
     5941 gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg ctatgcgtct gatctactgg
     6001 tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc
     6061 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6121 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6181 acaagaaagg cggtctagca aacgtggtag agaaagcgct cggctccatt gctaaatcgg
     6241 gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6301 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6361 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6421 tcattaaaat ggcaccccgc accgagctgg cgaaccgctg gtttgattta atggatatta
     6481 atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggagggctgg aagttgttcc
     6541 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6601 ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat tcgtattatg
     6661 cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg atccccgacc
     6721 agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca
     6781 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     6841 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggctt
     6901 aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca
     6961 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     7021 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     7081 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtacttg
     7141 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     7201 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagtgcg
     7261 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca
     7321 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc
     7381 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     7441 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     7501 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     7561 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc aagctactct
     7621 cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact attatctttc
     7681 gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa gatcaatatc
     7741 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     7801 agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     7861 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     7921 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     7981 tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg gtgaccatct
     8041 ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     8101 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     8161 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     8221 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     8281 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     8341 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     8401 tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg tcgaattcga
     8461 tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga
     8521 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatctct
     8581 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     8641 cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac cagcgaaagc
     8701 ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     8761 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca gctactcaga
     8821 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     8881 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc cgctgcagta
     8941 cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta taaaccatat
     9001 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
     9061 aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
     9121 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
     9181 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
     9241 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
     9301 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
     9361 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca
     9421 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
     9481 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
     9541 gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt
     9601 atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
     9661 ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg cgatacgctg
     9721 gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc
     9781 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
     9841 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
     9901 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
     9961 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10021 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    10081 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    10141 ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca
    10201 acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    10261 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    10321 gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac
    10381 tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga
    10441 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    10501 gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    10561 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    10621 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    10681 tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt
    10741 ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc
    10801 tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt
    10861 tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa
    10921 taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt
    10981 ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtagttaa
    11041 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    11101 gaccatgttc tggctggtac gccgcggcta gagtccgctg cgcctgattg cggttaccgt
//
LOCUS       DAJTUI010000113        11046 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTUI010000113 DAJTUI010000000
VERSION     DAJTUI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.113"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            409..1239
                     /locus_tag="OO464_003384"
     CDS             409..1239
                     /locus_tag="OO464_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6228416.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO464_003385"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO464_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR6228417.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="OO464_003386"
     CDS             complement(2368..3828)
                     /locus_tag="OO464_003386"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6228418.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO464_003387"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO464_003387"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6228419.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="OO464_003388"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="OO464_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR6228420.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO464_003389"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO464_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR6228421.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO464_003390"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO464_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR6228422.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO464_003391"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO464_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR6228423.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..>11046)
                     /gene="fimD"
                     /locus_tag="OO464_003392"
     CDS             complement(9005..>11046)
                     /gene="fimD"
                     /locus_tag="OO464_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR6228424.1"
                     /translation="IGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKW
                     QHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVI
                     HGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFT
                     VPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLA
                     DRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQ
                     LVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTV
                     TQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRD
                     QMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSY
                     SVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANG
                     VTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTL
                     ADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSS
                     GIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2685 a         2827 c         2682 g         2852 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gagtgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgtgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgacgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc
     3901 ggtgggtatt aacggataaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt tgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc gtcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaata gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtgga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatcc
//
LOCUS       DAJTUI010000114        11028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTUI010000114 DAJTUI010000000
VERSION     DAJTUI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.114"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..1599)
                     /locus_tag="OO464_003393"
     CDS             complement(<1..1599)
                     /locus_tag="OO464_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6228425.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKP"
     gene            complement(1533..2156)
                     /locus_tag="OO464_003394"
     CDS             complement(1533..2156)
                     /locus_tag="OO464_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR6228426.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2153..3841)
                     /locus_tag="OO464_003395"
                     /pseudo
     CDS             complement(2153..3841)
                     /locus_tag="OO464_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(3990..6617)
                     /gene="gyrA"
                     /locus_tag="OO464_003396"
     CDS             complement(3990..6617)
                     /gene="gyrA"
                     /locus_tag="OO464_003396"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6228427.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEYIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6764..7486
                     /locus_tag="OO464_003397"
     CDS             6764..7486
                     /locus_tag="OO464_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6228428.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(7614..11028)
                     /gene="yfaL"
                     /locus_tag="OO464_003398"
                     /pseudo
     CDS             complement(7614..11028)
                     /gene="yfaL"
                     /locus_tag="OO464_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2546 a         3081 c         2718 g         2683 t
ORIGIN      
        1 cggtttatcg gcggcgggga gatttaacgc cacatgaccg ctggcgtcgg acaccgtttc
       61 gctgccttcc agcgacacgg ggaaacgtcc ggcataacgc aaatcgttac cgaccattga
      121 taattgctga gcgcgcaaac ttaactgcac gcgggcattt tttaccggct cgccatccgg
      181 gtagagcagt tgcagtttgc cgctgaccgc ttcgccagtt ttgaactctt ttttgtcgag
      241 agctaaacca atctcgaaat gtggcttgat gtagtttgcc acgcgaaaac tgctgctata
      301 gacctgattg cggtaagcaa gacgtaactc ataacctccg gcaacggcat tttctggcag
      361 gcggaaactt ccctgcccgc cattgcgcgc atccagcgtg acatcgacgg tttgcaacag
      421 actgccgtta gcgtccagca ccgaaagctt cgccggggcg ctgacgatgg gggatgaatg
      481 caacggatcg cggaactcgc ggcccatcac tttaacatcg acacgatcgc ctgcgcgata
      541 tagcgggcga tcggtaaaaa tatacaagcg ggtgttgtag atttcgcttt cgtagaagaa
      601 gttctcggag acaaaaacgc cgccttcagc atccttaccc agaatgtatg aacgttctgg
      661 cgatatatgt tgtaactgca aagtaccgct gtcatcggtc accccgcggg tcatcacgcc
      721 aagaccttca gtccacaaga tctctgagcc gggcttcgct tcaccctgtt ttttacccgc
      781 ggtccatacc agaagctcgt tacctgacac tttgctaagc gccacggtat cggaaacaaa
      841 caccaccgtc gtcgcccgat acccacctac catcgcctcg acgaggtaca gccccggctc
      901 ttgttggccg agaggaatat aaatattacc cggctgcggc gagatgaaat tgctggatgc
      961 gccttccagt tttacccctt gctgcggctc gaccggttta gcctgccata gtggataacg
     1021 gaactgttcc accaagggat attttttcag cggggaaaac tggttgttct gtacataacg
     1081 ggaaggttta ataatggcat tgccgagatg taattcgggt aatgcctgag tcacattctg
     1141 ccgtgactga gaagagaaag tacgctgcat cacgcggcga gatttgccgt accagttatc
     1201 ccacagccag gtcagcgtat tgttcagccc gtcgcccaga tattgcggtt gcaccacaat
     1261 gcgatgcagg tttttctgct ggcgcaaaaa tgccatcggg tcaggaatac gatataggcg
     1321 aacgtccacg ccgccgtact cttccatctg atagcgccga taatcacgcc ccggcgcttc
     1381 cagtcgcact ttcgcctctt cactgctgct aaaactgctg tcagcaagca aaaagaatgt
     1441 tcccccggcg ggcggcgcat agttactgga aggaagcgaa tcatcagctt tagcaagccc
     1501 tgttcccaca agagacaggg acaagcacgc tgctatcgcg ccagaaaatt taaacgataa
     1561 atgccaatga aattgggatt ggaatcgttg ggtatccatc gggtgtcctt ccatgtcata
     1621 agttgttgca gactgactgc gcgcattccg ttgtcagttt tcgtggcgct tccggtgtgg
     1681 tagatgacgt aacgccccat ccagaccatt aagtgctggg catcgccctg atcgaaaaaa
     1741 atcatatcgc caggcagcgc ctggtttatg tcttggccaa taaactggct gttgtactga
     1801 atcaaattaa tcgcggtcac gtaggggccg tttttcccgc tcccctgatt ccagttttgc
     1861 gccagttgac gctgttcagg tgttagcgtc atctctggcg gcaaatactg gctagataaa
     1921 ccgttacttt ttagccattt actgtcgtga actttcagcg tctcgttcgc cgcaaatcgc
     1981 accaggcccg cacaatcctg ctgataccag cgtggacttg gcccctggcg gagctgttct
     2041 tgtgcaatgc gtacaaacca ggcgcgaaac agcctggatt gttcgacatt cagcatttcg
     2101 ctatgggcaa caacacaaca cagccagcaa atcagcgcca gcagcccgtg tctcatagcg
     2161 gctgccaggt tatgggtagc cactgccagg cgacaccggg ttccatctgg gccagcttca
     2221 tgacataacg cggttgttga gataaagcgt ccagcttcgg cattaataaa gtttgtgcgg
     2281 cgttataaaa aaccggttcg agattcttcg gcagactggt caacgtttcg ttacgcagca
     2341 gtttcgccac gccttgtgga ttgatataga gcggaacgat gccatcagtg ggtattacat
     2401 ccaccattgc cgggcgggtt ttattcagtg tttgcagggc gttattaacc agcgtgtcat
     2461 cgagggagaa aagcagcgtt ttgttttgca tcgccagcga cacacggaaa aaataatccg
     2521 acattaattg atcgggttgc gccgcctgcg ctttcggata ctgaccgtat cgggaactca
     2581 cttcgcgaca ccagatttgc gcttcgccct gctgagtctg gcttaccggc aaaacacctt
     2641 ctggcgcttt gctttcgtgc gcaccaatat tttgcgtaaa cagtttccct ggcaattgcg
     2701 cctgttcggc agtgccatca aactgaccga caaacagcgg ggtttgcaat tttgagtctt
     2761 cataccagca cagccccgcg gcaccgtcta acgccccatt cagcttgtcg ttttcctggc
     2821 tgatgtgcga aagcatctct tcggcaatac cgtgtgaata cggcaccgcc acacagaagc
     2881 tggctccggc aggcatactg ttccagaccg gcgtaaaatc gaaactggca tctacgctgg
     2941 cggattcatc atttaacgcc acaaaactgt gccagccgtc gttacccatt tcgaagcgta
     3001 cgccagcaaa agaaggcatt aaccgctggt agccaaaacc cagcaacctg gcgctgacca
     3061 cgatgcgcag gcgtactggc gttttttcgg ctgtacgctc ttccaggcca aagctggctt
     3121 gccagcgttt tttaccgctc aacaaatcac ttgcgatcgc cgtggcttcg gtatcctgct
     3181 gatcatcttt aaacaacata tccgtactgg aaaacaccag catcttgtcc tgatatgtcg
     3241 cgaacatcag ggcgttattg ccgttatagc gcaactgata aacaggaata gtttcactat
     3301 ttatcttgat gctactgatt tccgttttgc ttaactagct gtcgctggta gcggcaaaca
     3361 acaatggctc cagcagtttg cttaaaccac tgcgctggat cagcaccatg taatgtgaaa
     3421 gatggccttg tttatcgtgc catagcgccg cctgcgcggg ctgatctaag agtgacgaaa
     3481 agagcttatc tttcagcgtg agatcgtgtt catagacaat gcggcgaatg ctgccttcaa
     3541 tgcccagacg atcggcatga ttctgataat agaaaacgaa atcttcgctc agaacatcgt
     3601 ggagaaacgg aatggtgagg agatctttgg gaagctggct cagagagtcg ctgtctagaa
     3661 agaggtccgg ctcattgaga tcgatttgca gattgttgtg caccaccagc ggcgacaacg
     3721 ttttttctgg cccactgcca gcatattgca acgcccagac gccagcggaa agcagtgcta
     3781 ttgcgccaaa acctacaaga ccatagaacc gccagccttt cgcctttttt tcaccactca
     3841 ttgccacatt ccttgtgtat agccagccat tttttacggg cacagccaaa ctttaccgcg
     3901 ccctaatacg acaaaagccc agactttgca gcctggactt ttcaattcaa acaagggaga
     3961 tagctccctt ttggcatgaa gaagtaaaat tattcttctt ctggctcgtc gtcaacgtcc
     4021 acttccggag caatttcatc atccccttcc gcggcactgc cgtcgatggt atccagatct
     4081 tcctcgtcaa ccggttcagc aacacgctgc agacccacta cgttttcatc ttccgcagta
     4141 cggatgagga tcacgccctg ggtgttacgg cccacgatgc tgatttctga aacgcgagta
     4201 cgtaccagcg taccggcatc ggtgatcatc atgatctggt cgcagtcatc tacctgcacc
     4261 gcgccaacca ctaaaccgtt acgttcggta accttgatgg agataacccc tttcgtcgca
     4321 cgcgacttgg ttgggtattc cgccactgca gtacgtttac cgtaaccgtt ttgcgttgcg
     4381 gtgaggattg cgccatcgcc acgaggcacg atcagagaga cgactttatc gccttcacct
     4441 aagcgaatac cgcgcacacc agtggtattg cagcccatcg cacggacaga agactcttta
     4501 aagcgcacca ctttaccttc ggcggagaac agcattactt catcttcgcc actggtcaga
     4561 tcaacgccga tcagctcatc gccgtcaacc agtttgatcg ccactttacc ggcggtacgc
     4621 agacggttga attcggtgag gacggttttc ttcacagtac cgttagcggt cgccatgaag
     4681 actttcacgc cttcttcaaa ctcggtcacc ggcaggatcg cggtgatacg ttcgtcctgt
     4741 tccagcggta gcaggttgac gatcggacga ccgcgcgcgc cacgagtggc ttccggcaac
     4801 tggtaaactt tcatcgaata gacgcgacca cggctggaga agcacagaat atggtcgtgc
     4861 gtgttcgcca ccagcagtcg gtcgataaag tcttcttctt taatacgtgc ggcagattta
     4921 cctttcccgc cacgacgctg cgcttcgtat tcagaaagcg gctgatattt aacgtagccc
     4981 tggtgagaga gcgtcacgac cacatcttcc tgggtgatca gatcttccag gttgatgtct
     5041 gcgctgttgg cggtgatttc agtacgacgt ttgtcaccga actgttcacg aaccagctcc
     5101 agctcttcgc ggatcacttc catcagacga tcggcgctac caagaatacg caacagttcc
     5161 gcgatctgat ccagcagctc tttgtattcg tcgagcagtt tttcgtgctc aaggccggtc
     5221 agtttctgca aacgcagatc cagaatcgcc tgagcttgct gttcggtcag gtagtacaga
     5281 ccatcacgca cgccgaactc tggctccagc cattccggac gcgcagcatc gtcgccagca
     5341 cgttccaaca tggcggcaac gttgcccagc tgccacggat tagcaaccag cgcagttttc
     5401 gcttctgcag gcgtcggcgc atgacggatc agttcgatga tcgggtcaat gttcgccagc
     5461 gccacggcta atgcttcaag gatatgagca cgatcgcgag ctttacgcag ttcgaaaata
     5521 gtacgacggg tcaccacttc acggcggtga cgaacaaacg ccgcgatgat gtctttcagg
     5581 ttcatgatct tcggctgacc atggtgcaat gccaccatgt tgataccgaa agaaacctgc
     5641 aactgggtct gggagtagag gttgttgagc acaacttcac cgaccgcatc gcgtttcact
     5701 tcaatcacga tgcgcatacc gtctttgtca gactcgtcac gcagcgcgct gatgccttcc
     5761 acgcgttttt cttttaccag ttccgcaatc ttctcgatca ggcgcgcttt gtttacctga
     5821 tacggaattt cgtggacgat aatggtttca cgtccggttt tggcgtcaac ttccacttca
     5881 gcacgggcgc ggatatatac cttgccgcga ccggtacggt aagcttcttc aataccgcga
     5941 cgaccgttaa tgattgccgc cgtcgggaag tccggccccg ggatgtattc catcagccct
     6001 tcaatgctga tgtcttcatc atcgatatac gccagacaac cgttgatgac ttccgtcagg
     6061 ttgtgcggcg ggatgttggt tgccatacct acggcgatac cggaagaacc gttcaccagc
     6121 aggttaggaa ttttggttgg catgacgtcg ggaatttttt ccgtgccgtc atagttatca
     6181 acgaaatcga ccgtctcttt ttcgagatcg gccatcagtt catgggcaat tttcgccaga
     6241 cggatttccg tataacgcat tgccgccgca gagtcgccgt cgatggaacc gaagttaccc
     6301 tgaccgtcta ccagcatgta acgcagcgag aatggctgcg ccatacggac gatcgtgtta
     6361 taaaccgcca agtcaccatg gggatggtat ttaccgatta cgtcaccaac gacacgggca
     6421 gattttttat aggctttgtt ccagtcattg cctagtacgt tcatggcgta aagtacgcga
     6481 cggtgtaccg gcttcaggcc atctcggaca tctggcagcg cacggccaac aatgaccgac
     6541 atcgcataat ccagatagga gctctttagc tcttcctcaa tgttgaccgg tgtaatttct
     6601 ctcgcaaggt cgctcatcta accgctatcc ctctactgta tcccggattc aaaggtcgca
     6661 aattataaca cagccgcgca gtttgaggta aacctatacg ctttattcac atccaatgcc
     6721 tgatatactc gtttgtcttg ccaattacgg agtagaagtg ccaatgaatg ccgaaaaatc
     6781 gccggaaaac cataacgtag accacgaaga gatcgctaaa tttgaagccg tcgcctcccg
     6841 ctggtgggat cttgaaggtg agttcaaacc gttgcaccgc attaacccgc tgcgtctggg
     6901 ctatattgcc gagcgtgctg gcggtttatt tggcaaaaag gtgctcgatg tcggttgtgg
     6961 cggcggcatt ctggccgaga gtatggcgcg cgaaggcgcg acggtgaccg gtctggatat
     7021 gggctttgag ccattgcagg tggcaaagct gcacgcgctg gaaagcggca ttcaggtgga
     7081 ttacgtgcag gaaaccgtgg aagagcacgc ggcaaaacat gccgggcagt atgatgtggt
     7141 gacctgcatg gagatgctgg agcacgtacc cgatccgcag tcagtggtca gagcctgtgc
     7201 gcaactggtg aaaccaggcg gcgatgtctt tttctcgacg cttaaccgca acggcaagtc
     7261 atggctgatg gcggtggttg gcgcggaata tattttgcgc atggtgccca aaggtacgca
     7321 tgatgtgaag aagtttatta aaccggcaga gctgcttggc tgggtggatc agaccagttt
     7381 aaaagagcga catataaccg ggctacatta caacccgatc actaattcct ttaagctcgg
     7441 ccccggcgtg gatgtgaact atatgctgca cacgcagaat aagtgaggtt gatgtttggc
     7501 cgcgccaatg cctgatgcga cgcttgcagc gtcttatcag gcctacaaat gctctccgta
     7561 ggccagataa ggcgtttacg ccgcatccgg caattgtgat ggtcagacag cgattaccat
     7621 ttcaccgtca tcgacaaaaa ccctgccgtc tgggcaaaat catcactccc tttctgccac
     7681 gccacgctgc cgcgcaggga cactcgctga ctgatattgc ccgtgactcc cacttttatt
     7741 tcaccccgta gcttcaccgc atcgtcactg atagtgctgc catcttcttc aatttccgtc
     7801 gcatggggat cgtgatagta attcagatct aatgttggtg tgacaccaac cgcggtacgc
     7861 cattcgctgt gtaaacccag ccgcgtctga atatcatccc cctgcgattg tgacacgcgc
     7921 gcatggttag cggcggtaaa atcatcctgc tgcacgccct gataaatcac ctgcgcctgc
     7981 ggttcaatca ccacaccacg ccccggtaac cactgatacc cagcttccag cgaggcgata
     8041 atccccgacg aatggtaacg atccacgcca tcttcatgtt cggaaacatc attgttaaac
     8101 cacgcgtatt gcagccagct atccagccag gccccctgct tctgattacc gtgctgatac
     8161 cagcttgagg tcagcccaac ggcataaccg tggttctggt tatcggcgcg agttccggtc
     8221 atattcgagc ggctgtcgcc ctgattatca ctgtagccac caaccgcccc aagcatccac
     8281 tcgccatccg tgccccagcg cccgctaaac aagccgccgc taagctgcac cgtagaagtg
     8341 tcttcctgct gagccagttg ccctgccgcc gtgtaatgat aacgtccgcc gataacacgt
     8401 aaattcagcg tctgaccatc gccaccagcg tgatcgcgtc gctccatcat aaacgcctga
     8461 tttgccgccc gcaggttatt gagataaccg ccaactttgg cattcagcac cggctggtaa
     8521 gcaggcgtag gggccgggtc gggggttggg tcaggatccg gcgtgggatc aggatcggga
     8581 gtcgggtctg gctcaggtgg cgatggcggc gttacttctt gcgatcgcag ataccagtcg
     8641 ttgttatctt ccactagcgt gtagtcatac gctcccatgt tgacgtagcc actgccagcc
     8701 agactgaact gtgcattgtt ttgaaactgc gtgggatcag ctgcgaaatc aaccacttta
     8761 atgcctgtcg atgtcggctc accaatccct gtaatggagt taaccaccac agttgtgttg
     8821 ccagcagtat taccgttcat caccaattga tcgcttaccg agtcatcgcc gtttaattcg
     8881 ctgtcgaata gtagcgtacc gccgccggta taatcaccgt ttaccgtcag cgtatcgcca
     8941 gcgacgccat tttgcaggct aaccgcaccg gacgtattgg tcagatcacc attaaccgtc
     9001 acgtcagcaa gataggtcag gctgtcagtt tcaaggttgg tattaagctc gcctgtcaac
     9061 gtcacgccat cggtagcatt taccagcgtc ccatcgccgt taaatagtga ggcattaagg
     9121 gcgacggaag tatcctgccc ggtcagacgc aaaaccgtac catcgctgat gtcgatagtg
     9181 ccgctggaag tagcatcaat tgactgaata tcctgatccg cgccagtaac gaacgttgcc
     9241 ccatcaccaa cccacagtga cgaagcataa ggaaagatat ccgcaacatc gcctttcagg
     9301 gtgccttctt cgacacgtac cgccgactgc gttgtaccgc tggcggtcag ctccagcgta
     9361 cccgcccccg ttttagtaaa tgtgctaccc tcatcctgat gctgcccgct gctgtcagcc
     9421 atcagtgcgc cccactgcgt gtctaccccc gcatcaactg ccacttcacc gtcggcgcgc
     9481 atttcaatat cacgcccatg ctcagtcgtc gtagagcgag tatcgacatt ggcagtaatc
     9541 tccatcacgc tttcttgtta cttatcggta aagataacct ggcggttata gtgggtatct
     9601 ccaagctgcg agttgtcgct caccatcagc gtaccggagg cgatttgtgt tgtgcccaaa
     9661 taactattat tgtttgccag acttacctgc ccgtcgctcg catcgaccac cacgtagacg
     9721 ccatctttcc cgtcgccgcc gatatgcaaa ttgtcgccac ctgcaagatc taccacatcc
     9781 tgactgccga taaccgttcc gctgctgcca ctgacttcaa ggccatcatt gtatgatgtg
     9841 ccgctctgcc aagtggagaa atcagagaga tcgagcacac cgccgtttaa cacgatcgac
     9901 tgcggatcgt cctggagagc ggtaagatct gccgcgtcgc cttccagcga aagcaccgca
     9961 ccatcatcaa cgactatatc gccggttagc gccatcgact gcgcccctgc cagcacgtag
    10021 ctgccgtttt gcgcaatggt gagctgacca gcaccttcga tgatgccagc aaaactgccc
    10081 tgattaacag taacgttgcc accagcatcg atatttaaag tgccattctg aaagcccgtc
    10141 aatgcgtgca caaaagtttg ttgggtcgag ccaacgttta gctcagcctg attctgatac
    10201 tgatcaatac tccctatcgt cagaccgtag cagtcttgcg gatcgtcctg gcaatgcgta
    10261 tcgccgacat tcatcaggga gttgctgcga cccagggtaa cttcaccgtt ttcaatctgc
    10321 atctcaccag taaagtcatt gttatctgca ttaagtacca gatcgccgga acctgttttg
    10381 gtgattaacc cggtaccagc aatagagtca acagctccgt cattctctgt attgccgata
    10441 accagcgttt ttccgtcggc aatatcaaag gtaacttcac ttaagccgag atacataaag
    10501 ccacccgccg cagtggaggg accatctcca tagcctgctg cgctattgtt ctcatcgact
    10561 aacacgccgc cgttctggct gtagctgtca tcaacagaaa tatcaataag atagggagcc
    10621 gtcgcgctat tggtatatat cgccccgcca taaccttcgg ctgtgttatt tgtaaaggcg
    10681 gtgttattta ttatcgtata acctgaagga tgcttgctgt cgctattatt atcggtaaca
    10741 tcgattgccc cgccatcgcc atcactgtaa cttgttgatg tatatgcctg gttattgtta
    10801 aaaacatcat cacttaaata gacgtcatta ttaatggtat aaattgcgcc acctttgcca
    10861 tcattagcga tattattgat ggcgttagtg acacgtaaat cgacggcacc agtatcatta
    10921 gtaccagaag aatagattgc gccaccatag ccgcctgcga cgttaccgga aaaaataaca
    10981 tcagtaagat tcagcgttga gttttcttta gcaaatatgg ccccgccg
//
LOCUS       DAJTUI010000115        10963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTUI010000115 DAJTUI010000000
VERSION     DAJTUI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.115"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..241
                     /locus_tag="OO464_003399"
     CDS             <1..241
                     /locus_tag="OO464_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228429.1"
                     /translation="NGSVRYCQYTSMFGASYRYSGAGVGYQVASVVGGGFTPFIAAAL
                     ITYFAGNWHSIAIYLLAGCLISAMTALLMKDNQRS"
     gene            343..1797
                     /gene="amn"
                     /locus_tag="OO464_003400"
     CDS             343..1797
                     /gene="amn"
                     /locus_tag="OO464_003400"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6228430.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2140..2856
                     /locus_tag="OO464_003401"
     CDS             2140..2856
                     /locus_tag="OO464_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6228431.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3312..3387)
                     /locus_tag="OO464_003402"
     tRNA            complement(3312..3387)
                     /locus_tag="OO464_003402"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3352..3354),aa:Asn,seq:gtt)
     gene            complement(3488..5115)
                     /gene="yeeO"
                     /locus_tag="OO464_003403"
                     /pseudo
     CDS             complement(3488..5115)
                     /gene="yeeO"
                     /locus_tag="OO464_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5120..5195
                     /locus_tag="OO464_003404"
     tRNA            5120..5195
                     /locus_tag="OO464_003404"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5153..5155,aa:Asn,seq:gtt)
     gene            complement(5233..6183)
                     /gene="cbl"
                     /locus_tag="OO464_003405"
     CDS             complement(5233..6183)
                     /gene="cbl"
                     /locus_tag="OO464_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR6228432.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6285..7202)
                     /gene="nac"
                     /locus_tag="OO464_003406"
     CDS             complement(6285..7202)
                     /gene="nac"
                     /locus_tag="OO464_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR6228433.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7530..7605
                     /locus_tag="OO464_003407"
     tRNA            7530..7605
                     /locus_tag="OO464_003407"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7563..7565,aa:Asn,seq:gtt)
     gene            complement(7661..8596)
                     /gene="ldtA"
                     /locus_tag="OO464_003408"
     CDS             complement(7661..8596)
                     /gene="ldtA"
                     /locus_tag="OO464_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6228434.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8658..9737)
                     /gene="cobT"
                     /locus_tag="OO464_003409"
     CDS             complement(8658..9737)
                     /gene="cobT"
                     /locus_tag="OO464_003409"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6228435.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9749..10492)
                     /gene="cobS"
                     /locus_tag="OO464_003410"
     CDS             complement(9749..10492)
                     /gene="cobS"
                     /locus_tag="OO464_003410"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR6228436.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10489..>10963)
                     /gene="cobU"
                     /locus_tag="OO464_003411"
     CDS             complement(10489..>10963)
                     /gene="cobU"
                     /locus_tag="OO464_003411"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR6228437.1"
                     /translation="SQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELI
                     HADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRC
                     PAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3046 a         2787 c         2373 g         2757 t
ORIGIN      
        1 aaatggtagt gtccgctatt gccagtacac ctcaatgttt ggtgccagtt atcgctacag
       61 tggtgctgga gtcggttatc aggttgccag tgtggttggc ggtggattta caccttttat
      121 tgccgctgca ctcatcactt actttgccgg gaactggcat agcatcgcca tttatttgct
      181 ggctggatgc ctgatttctg caatgaccgc tttattgatg aaagacaatc aacgctcgtg
      241 atagcctggc gaagatcatc cgatcttcgc cttatacttt tgtttcacat tcctgtgaca
      301 tactatcgga tgtgcggtaa ttgtatggaa caggagacac acatgaataa taagggctcc
      361 ggtctgaccc cagctcaggc actggataaa ctcgacgcgc tgtatgagca atctgtagtt
      421 gctttacgca acgccattgg caaatatatt acaattggcg aattacctga tgaaaacgcc
      481 cgtaaacaag gtctttttgt ctatccatca ctgaccgtaa cctgggacgg tagcacaaca
      541 aatcccccca aaacgcgcgc atttggtcgt tttactcacg caggcagcta caccaccacg
      601 attactcgcc ctactctctt tcgttcgtat ctgaatgagc aactaacgtt gctttatcag
      661 gattatggtg cgcatatctc tgtgcaaccc tcgcaacatg aaatccctta tccttatgtc
      721 atcgatggct ctgaattgac acttgatcgc tcaatgagcg ctgatttaac tcgctacttc
      781 ccgacaacag aactggcgca aatcggcgat gaaactgcag acggcattta tcatccaacg
      841 gaattctccc cgctatcgca ttttgatgcg cgccgcgtcg atttttccct cgcacggttg
      901 cgccattata ccggtacgcc agtagaacat tttcaaccgt tcgtcttgtt taccaactac
      961 acacgttatg tggatgaatt cgttcgttgg ggatgcagcc agatcctcga tcctgatagt
     1021 ccctacattg ccctttcttg tgctggcggg aactggatca ctgccgaaac cgaagcgcca
     1081 gaagaagcca tttccgacct tgcatggaaa aaacaccaga tgccagcatg gcatttaatt
     1141 accgctgatg gtcagggtat tactctggtg aatattggcg tgggaccgtc caatgctaaa
     1201 accatctgcg atcatctggc agtgctacgc ccggatatct ggttgatgat tggtcactgt
     1261 ggcggattac gtgaaagcca ggccattgga gattatgtcc ttgcacacgc ttatttacgc
     1321 gatgaccacg ttcttgatgc ggttctgccg cccgatatac ctattccgag cattgctgaa
     1381 gtgcaacgtg cgctttatga cgctaccaag ttagtcagcg gcaggcctgg tgaggaagtc
     1441 aaacagcggc tgcgtacagg tactgtggta accacagatg acaggaactg ggagttacgt
     1501 tactcagctt ctgcacttcg ttttaactta agccgggccg tagcaattga tatggaaagt
     1561 gcaaccattg ccgcgcaagg atatcgtttc cgcgtgccat acgggacact actgtgtgtt
     1621 tcagataaac cgttacatgg tgagattaag cttcccggcc aggctaaccg tttttatgaa
     1681 ggcgctattt ccgaacacct gcaaattggc attcgggcga tcgatttgct gcgcgcagaa
     1741 ggcgaccgac tgcattcacg taaattacga acctttaatg agccgccgtt ccgataataa
     1801 gttatgaacc ttcttatcag aaatagatgt gaaggattaa gtaagacatc tggcaaatag
     1861 cctgcaataa acgcgggctg cttgcttcct tgggcagatg cgagttttat tatcgtctta
     1921 atgatttcca catattaaaa gcaagtatgc tttcaaaaca caattataaa aaatcccgcc
     1981 aacaacatta gtttttataa aattaaatat aagattatgg ctttagaata tttttatttc
     2041 taatagacga gatttttcct gttatgatat aatatgccga attaacacat gttaacgatt
     2101 taccagtaat gtaaataaat tttcgaggag atcattccag tgggacgtaa atgggccaat
     2161 attgttgcta aaaaaacggc taaagacggt gcaacgtcta aaatttatgc aaaattcggt
     2221 gtagaaatct atgctgctgc caaacaaggt gaacccgatc cagaattaaa cacatcttta
     2281 aaattcgtta ttgaacgtgc aaagcaggca caagttccaa agcacgttat tgataaagca
     2341 attgataaag ccaaaggcgg cggagatgaa acgttcgtac agggacgtta tgaaggcttt
     2401 ggtcctaatg gctcaatgat tattgctgag acattgactt caaatgttaa ccgtacgatt
     2461 gctaacgttc gcacaatttt caataaaaaa ggcggcaata tcggagcggc aggttctgtc
     2521 agctatatgt ttgacaatac gggtgtgatt gtatttaaag gaacagaccc tgaccatatt
     2581 tttgaaattt tgcttgaagc tgaagttgat gttcgtgatg taactgaaga agaaggtaat
     2641 attgttattt atactgaacc tgctgacctt cataaaggaa tcgcggcgct aaaagcagct
     2701 ggaatcactg agttctcaac aacagaatta gaaatgattg ctcaatctga agttgagctt
     2761 tccccagaag atttagaaat ctttgaaggg cttgttgatg cccttgaaga tgacgacgat
     2821 gtacaaaaag tttatcataa cgtcgcaaat ctctaattat attttaaaga aatctgtctt
     2881 tatggcagat ttctttaatc tcatataatt cttataaaaa atataatatt taactcgtca
     2941 tattgcttat aaaaaatccc cgtccccaga gaaataatat ttattaaaat ggcaggtctt
     3001 ctttttctga ttaccgaaga caaagtggca attacgtata gtttcccgat aaagacgcga
     3061 cagctacgtc ccgcataagg catttttctc tttatctttg tacggtactt catggaacag
     3121 aatttttgac cttgtgaatc gtgatatctg ttggggaggg gcaatttgct cactgaagcg
     3181 tgagactcga ttaagcgcac gaaacacaga aatcaaaaaa cccggccact tttttacaaa
     3241 gtggccgggt aaaaataatt tttatttttt aactgtcctg agtctcatag agatgtctca
     3301 aaactaaaat ttggctcctc tgactggact cgaaccagtg acatacggat taacagtccg
     3361 ccgttctacc gactgaacta cagaggaatc gtgagaacga ggcgaatatt agcgatgccc
     3421 acccacaatg tcaaagcctg ttttttaaat ttgaaatcgt ttgctgaaat aatctgcatt
     3481 ttgtcgttta ttccgacaca actggctttt tttcacactt ttgcggctcg gatcgagggt
     3541 atttccacag ccaacgtcca gtaaccattc gccagtaaaa cagcacggcc cgcacagccc
     3601 agtcggcaaa cattcccatc cagacaccaa ccacacccca gccaagcatg attccctgca
     3661 cataaccgac tacaacccgg caaccccaca tgctcaacat agaaacccac atggcgtaac
     3721 gggcatcacg agcaccctta aatccagcgg gtagcaccca tgaggcggac caaataggca
     3781 taaataaagc atttagctaa atcagaatca caacgacgtg tttaacctgt ggatcctggg
     3841 tgtaaaacga tgccataacc ccggcaaagg gagccgttag ccaggcgatg gccgttaatc
     3901 caagagtgga cagccagaac acatgccgca actgaatctc tgcttgcgct atctgcccta
     3961 cccccaacct tcggcctgta atgatcgtag aagcagaacc gagcgcactt ccgggtaagt
     4021 tgataagagc cgcaattgag aacgcgataa aatttccggc aataacgctg gtccccatcc
     4081 cggcaacgaa catttgggtt aataaccgac cactggtaaa taacactgat tcgacactcg
     4141 ctggaatacc aatccccatg acttcccaga taatgctaaa attcagcggt ttaaaatagc
     4201 tctttaacga aatccttagc gcaggattaa aaccaatcgc cagcacccac aaaattgcaa
     4261 ctgcgccaat ataacgagaa atggttaaac ccaacccagc cccgaccagg agaaaaggcc
     4321 gtaaatcaat atgccgctaa taataatatt aagaatattc aggctaccgt taatcagtag
     4381 cggtattttc gtattccctg caccacgaag tgccccgcta ccaataagag tgatggcagc
     4441 ggctggataa ctgagtacgg tcagctccag ataagtcaac gccagtgctt taacttctgt
     4501 cgtggcatca cccgcgacga aatcaataat ttgttcgcca aaatgatgaa ttagcgttgc
     4561 caacagtacg gcaaacaacg tcatgatcac caatgactgc cgcgtcgcca ccctcgctcg
     4621 tcgtcgatcc cgcttaccga gactaaatgc tacaacgaca gtagtaccaa gatcgatagc
     4681 tgcaaaaaaa gccataatga ccatattgaa gctgtccgcc aaccccacgc cggccatcgc
     4741 atcttttccc agccaactga ccagaaaagt gctcaggacc cccatcaaca ggacacaggc
     4801 gttctccatg aagataggaa cagcaagcgg ggttatctcg cgctagaaca acactttgta
     4861 gctcttgcgt ttagagtgcc agcgagtgcc gtgaacaacc tggcgtaaag cagaggagat
     4921 attcaaagcc gaccttaatt gcagaaagtg aaaccacatt tcaaatgatg agggagaatc
     4981 agcaaagctg caaagatttt cgccaacaaa ttgtctgcaa atgcaacaaa ctgttgatag
     5041 aaacggcaaa cagttgggaa atttaaaaat cgggtttgac aaaatatttt tcgccgttaa
     5101 gatgtgcctc aacagcgatt cctctgtagt tcagtcggta gaacggcgga ctgttaatcc
     5161 gtatgtcact ggttcgagtc cagtcagagg agccaaattt ctgaaaagcc cgcttttata
     5221 gcgggctttt tgctatatct gataatcaat ttcctcttca ctgttttcca tcacctggcg
     5281 cttgatatcc tcaactgaca gtcctgcatt acaaagttcc agaaagcgcc aaacatagtt
     5341 acgctgaagt tgtcctcgct tcagtcccaa ccagacagta ttagcatcga aaagatgccg
     5401 cgtatccagg cggattaaat tctcttcctc ttgttcgccg ctggattgtt cggcaactaa
     5461 tccgatccca agcccaagag caacataggt tttaatgaca tcagaatcct gtgcacttaa
     5521 tacaatatct gccggcaaac ctttgcgggt aaatgcgtca tcagtgcgtg agcgccccgt
     5581 aattccctgt cggtaagtaa ttaacggcca cttcgctatt gattccagcg tcaatggtgt
     5641 aatttgcgtt aagggatgat cgagtggaac aagcaaacta tggtgccaac aaaaccatgg
     5701 gaaggcgacg agctgcgggt cattactcaa acgctcgctg gcgataccaa tatcagcttc
     5761 gccattttgc aacaacgtcg caatttcctg tggcgaaccc tggattagct cgagccgaac
     5821 ctccgggaaa agttcgcgaa aagctttaat gacctctggc aagctataac gtgcctgagt
     5881 atgcgtcgtt gcaatagtga gaacaccaga cgtatcgttg gtaaacaggt ctgcaagccg
     5941 acgaacatta ctggcttcat tcagaatacg ttctgcaatg accagtaatg ctttgcccgg
     6001 ttcagtcatc cccagcagtc gcttacctcg tcgaacaaat atttcgatgc caaattcatc
     6061 ctccagttcc cgaatatgac ggctaacgcc tgactgtgag gtaaaaagca tattcgcaac
     6121 ctctgtcagg ttgtaatcct gacgtgcagc ctcgcggatt atctttagtt gttggaaatt
     6181 cacggtaaac tccgggcagt tcagatatcc cgttattgtt aaagtctaat gcccggcata
     6241 acaaataata aaaacccgca tcttattcca tcccgatata acgcttagct caccaattgc
     6301 cactgccttt tttccatcac tggagaacta atcactgaca ttaacaactc tttcactgcc
     6361 tgtgcctgtg gtgacaagtt tgctctggcg ggtaaattta aggacaaaga gagactcatt
     6421 gaaggagtgg taatgcgtga catccaccca tttactgcgc cacataacga acgcgcagcc
     6481 gattcaggta atactgcaac gcccatgccg ctggcaatcg ctgcggtaag cgtggcaata
     6541 gactcaattt caccaatgac ttttgccgtg agccgccgta gggaaaaagc ctcatcaaca
     6601 cgaagtctaa cagcgctgta atcactgggg agaaaaaggt tcatttgcgc aatagcatta
     6661 acatcaacgt tttgccccgg gcaatcttga gttcctacca ggaaaagatc ttctttcagc
     6721 aaagcctgac tggatacccc agccacaggg gaatgctcat aaatcaccgc catatcgagt
     6781 tggtgattta tcagtttttc gttaagcact gcaccactat tttcatgaag atagataacg
     6841 acctccggga attcagcgcg aaccgcctgt aataagggca tggtgatgga tgacgcagcg
     6901 gttcctggcg caaagccaat cgagacttgc cccgataatg actgaccaac gttatgcacc
     6961 gccagttggg cctgctcaca ctgacgtaaa atggcccgtg catgggtata gagaattttt
     7021 ccggcgtctg ttggcgtaac gccccgtttt gtgcgaatca aaagctgttg atttaactca
     7081 ccttccagta tggcaacctg ttggctgagc gctggttgtg cgatatgcaa tacttcagca
     7141 gcctgggtca ggctaccaat atctacaatt tttacgaagt atttcaggcg tctgaagttc
     7201 atgttgcctc cggtttttaa gaatcggccc aagtgccgcc attacttaca accagtatgc
     7261 aagatgcctg ccagttctat tttggcgttg atgcgcaagc taaccaactg tcaaataaga
     7321 gattatgata gattcgtcat tggccctttt tatcagcagt cgcattcacc tgccctataa
     7381 aaggagggta tgcaccacga tggttcatta cccaataaga ttgaaagctc accactttgt
     7441 tgaaattggc agcaaacaaa caaaaaaaat gcatttcacc ctttgacatc accatgcact
     7501 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     7561 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     7621 gctttcgagc aggcttttta ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     7681 cagaactaat ttgcacaggc attcccgatc gatgttgcaa cgcagcattt gcgcgattta
     7741 catcaacttc ttgcccgttg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     7801 ttcggtcaga ctcatattct gcacgattgc gcgacagcgg ctcatgaact tccagccact
     7861 ttgaaccatc cggttccgtt gtgtatttca ctggctgatc aataatttgt acacgagtgc
     7921 ctacaggaac attatcaaac agatatttga tatcgtcatt acgcagacga atacagccct
     7981 gacttacacg gagcccaata ccaaaattgg cattggtacc gtggatggca tacaacctgc
     8041 caatataaat cgcgtacagc cccatggggt tatcgggccc cgcaggaaca aatgcgggca
     8101 aactctcccc tcgtttcgca tattcgcgcc ggatgttcgg cgttggcgtc caggttggtg
     8161 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     8221 cgataccaat aggaaagact tccactgtat tactgtctgg cggatagtaa taaagacgca
     8281 tttcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     8341 gaatggtgag ttgtgagcca ggcttcggca aaaaaacatc agcgcccgga ttcgcttcca
     8401 gcatattact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgagtattgt
     8461 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     8521 gataagttac cgccaggcta ttttggctgg caaaaagcag agcaaatgag caaagaatat
     8581 ttacacgacg catcatgtcc ctttcctaag tcgcgaatac tatccgataa gtatagcttt
     8641 tatcagactt ttcgttttta gctgttcaaa tcagaagtcg tattccccgg tagaacaata
     8701 ttactggcag caagttcgcc catgttgttg tatatcgcac aagcagcttc gatgatggac
     8761 atcgccagag ctgcaccact cccctcacct aaacgcatat ccatattgag ataaggctcc
     8821 agccccaaat gcgagagcgc tatacgcgcg cctttttctg ccgacaagtg agaaggaatg
     8881 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcagcagc ataagaaaga
     8941 aatccatcca gcaacacggg taaaccgcag gaagcagcac ctaacatcac tccagctatt
     9001 ccgaccaaat caaatccacc cacttttgcc aggacatcaa caccatcctg aggatttggt
     9061 tgattcaacg taatcgcccg acgcacaaca tcaattttat tagccagttt atctgtcggc
     9121 aggtttgcgc caatcccaac cacttcttca ggatcccggc cagtgattgt gctgactatt
     9181 gccgctgccg gtgtcgtgtt tgccatcccc agttcaccta caccaaacag cgtgacaccg
     9241 tttttcgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc agcctgacgg
     9301 cgactcattg ccggagctga agcaatattg ccgctacctc gtgcgacacg catgttgata
     9361 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtggac gttagcgccc
     9421 gcttgtgctg ccagcacaca cacgccggtt gttccacggg tcatattttc agcctgtatg
     9481 gctgtcactt cttttggaga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     9541 ataaccagta ccgctttttt gcccacatgc ggtatgccat tcacccccgg cattcctgcc
     9601 agttgtatgg caagcgcctc cagctttcct aggctaccaa caggtttgaa taacccgtca
     9661 acatgccgtt gtgcacgcga catagcggca gaattgatgg ctgggatcgt attcagtaaa
     9721 tcggcaatta tttgcatgtc acgtttcttt atagcagtgc cagcaggaag accagttcac
     9781 caagctctat ggctgctccc agcgtatcgc ccgtttgccc gcccagcgta cgtttgagaa
     9841 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     9901 ccagcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     9961 catcaatttt gccaatgaac acattgccaa gcccttcttc ccgcgcataa cgatgacgat
    10021 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
    10081 tcggttcgcc acgcagagcc aactcgctta acaccagaat ctttgccagt accacaaaaa
    10141 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
    10201 cgcgactacg tgcagaaaat acgccatcac aggtatcagc gaggccgtcc agatggaatt
    10261 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt gcgccacacc
    10321 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaatcaaag
    10381 gaaaagtaat aataccgcga gaataatgtt cgaaatccag tccctgggac cagcgacgcg
    10441 gtacaggcag acgcgtaata aatgagagca tcgcccaaaa taatttgctc atttgatttt
    10501 tactccaata cccgaaacca ccagccatac ttcatttgcc gcagcggcca actgctgatt
    10561 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
    10621 tcccacttcg ttagtcacta atacaacctt tgcggggcaa cgttggcagg cagcaatcaa
    10681 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
    10741 tttatcgccg ccataatcaa acaacagatt agtcaccatt gtggtaacgc attccagcaa
    10801 cacagcctca ttcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
    10861 cactgtgcgc cagtgctccg ggcggctttg ccgatgatgt tctatccgtg cagccatctc
    10921 atcatcaagg atttgcgagg tagcgatata cagaacctgt gaa
//
LOCUS       DAJTUI010000116        10916 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTUI010000116 DAJTUI010000000
VERSION     DAJTUI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.116"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            144..494
                     /locus_tag="OO464_003412"
     CDS             144..494
                     /locus_tag="OO464_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR6228438.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            547..942
                     /locus_tag="OO464_003413"
     CDS             547..942
                     /locus_tag="OO464_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR6228439.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            983..1726
                     /gene="cmoA"
                     /locus_tag="OO464_003414"
     CDS             983..1726
                     /gene="cmoA"
                     /locus_tag="OO464_003414"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6228440.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1723..2694
                     /gene="cmoB"
                     /locus_tag="OO464_003415"
     CDS             1723..2694
                     /gene="cmoB"
                     /locus_tag="OO464_003415"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6228441.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2859..5288)
                     /gene="torZ"
                     /locus_tag="OO464_003416"
     CDS             complement(2859..5288)
                     /gene="torZ"
                     /locus_tag="OO464_003416"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR6228442.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5313..6413)
                     /locus_tag="OO464_003417"
     CDS             complement(5313..6413)
                     /locus_tag="OO464_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6228443.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6801..7547)
                     /gene="cutC"
                     /locus_tag="OO464_003418"
     CDS             complement(6801..7547)
                     /gene="cutC"
                     /locus_tag="OO464_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6228444.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7561..8127)
                     /locus_tag="OO464_003419"
     CDS             complement(7561..8127)
                     /locus_tag="OO464_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6228445.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8343..10076
                     /gene="argS"
                     /locus_tag="OO464_003420"
     CDS             8343..10076
                     /gene="argS"
                     /locus_tag="OO464_003420"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6228446.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10253..10741
                     /locus_tag="OO464_003421"
     CDS             10253..10741
                     /locus_tag="OO464_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR6228447.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2667 a         2787 c         2602 g         2860 t
ORIGIN      
        1 ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact catagctaac
       61 cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta ggaaccatct
      121 gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt catgcatcac
      181 gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt ccggtacatg
      241 ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat atgacgcaaa
      301 accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag accactacgc
      361 cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta cataccctgt
      421 gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt ccacagcaat
      481 cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat tttaaaggga
      541 gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat gaagttctct
      601 tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg tggttttagc
      661 gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc catcgcgatt
      721 gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca tatttgcggc
      781 atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg tctgtttcgc
      841 tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt gctggcgaat
      901 ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat acgccacttt
      961 ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg cccctatcgc
     1021 cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg atatgatcca
     1081 gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag ccgagcgctt
     1141 cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg cgacgctctc
     1201 ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca actccccggc
     1261 gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc cagtagacgt
     1321 cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg tgctgaattt
     1381 taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa tttatcaagg
     1441 actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag atgccaaagt
     1501 tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt acagcgaact
     1561 ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt ccgtggaaac
     1621 ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt tccagtgctt
     1681 taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga ctttggtaac
     1741 ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct gcccgcgcag
     1801 attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc caacacggtg
     1861 gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt aaccgccgaa
     1921 agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat gcggaacctg
     1981 atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac cgaatggcgt
     2041 tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg acgcaccatt
     2101 ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc aggcgcgcac
     2161 ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc ggtgcgtaaa
     2221 ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca gcttccggca
     2281 ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg ttcgccgctg
     2341 gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt gctggaaacg
     2401 ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta cgcgcaaatg
     2461 cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa gaagtgtggt
     2521 tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca gcgacgcact
     2581 gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc gagtaagaca
     2641 gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc gtaaaggtct
     2701 ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct tgctaattca
     2761 atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt tttacgtttg
     2821 cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac agcaggctta
     2881 tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac cagcgcagag
     2941 ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa cacgttcgca
     3001 ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg cacgcatacc
     3061 acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc acgtttgttc
     3121 cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc ggtgtgaata
     3181 gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata gttaagctga
     3241 ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc gtcggcggta
     3301 cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt atagccaaat
     3361 ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag cgcatttttc
     3421 accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc gctgcggcgc
     3481 atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt gacgcgttgc
     3541 gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat ttcatcttta
     3601 ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc ggcaaacacg
     3661 tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg cacaatatgc
     3721 tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt ggtgatcggt
     3781 aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac gacgatcatc
     3841 tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt aaagttaccg
     3901 ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc gttatgctga
     3961 tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat cgccgtcatt
     4021 ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat ttccggcagc
     4081 acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa accaaaaccg
     4141 ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat ccagtgtttc
     4201 tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt gcgattcgcc
     4261 gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat ttccgccgtc
     4321 cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc aaactgcgga
     4381 tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt catcagggta
     4441 tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat ccaggtggca
     4501 ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat cactggtttg
     4561 ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact gctccaggca
     4621 atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct gttttccaga
     4681 atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac atgcggcatg
     4741 atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc gcccgccagg
     4801 ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga gcgccagccg
     4861 taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc atgttgctca
     4921 tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc gccacgacct
     4981 ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg ctgaatacgc
     5041 gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat ggttttcgcc
     5101 agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat cgctccccaa
     5161 cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag cggtgctgcc
     5221 gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc acgtcttgtt
     5281 aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat atctttggcg
     5341 ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct ggttcgtgcc
     5401 cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc ggcaatagag
     5461 gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca tagcggttta
     5521 cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg ccattgattg
     5581 ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc ttcttcactt
     5641 aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc actgccctgc
     5701 atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac gacggtgaga
     5761 tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa aggataaata
     5821 tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc cgcgttgtca
     5881 tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat acaggtttcg
     5941 ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga ggcaatatcc
     6001 atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt taattctttc
     6061 catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact gtctattttg
     6121 ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa taaataatcc
     6181 atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt ctggttcgaa
     6241 aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca ggaaaggcaa
     6301 aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc gccgccacca
     6361 accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg catagattcc
     6421 tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac gaattaaccg
     6481 cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga tttattacaa
     6541 tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata tttatcagtt
     6601 ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc acaacgctat
     6661 gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac tgctcaggga
     6721 taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc atattgggcg
     6781 acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc tttcatttca
     6841 gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc atctgatgac
     6901 atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc cgcggagcta
     6961 tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg gactcctgct
     7021 ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt tgataaacct
     7081 tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa ttccgcaaga
     7141 ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa agtcactgcc
     7201 agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt cccgtcaaca
     7261 tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg cacatcctca
     7321 agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg gcgaattatc
     7381 ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa cgacggcgtt
     7441 aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt ttgctgcgcc
     7501 gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt tgctccttaa
     7561 ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa acttaatagt
     7621 tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct caccttttgg
     7681 tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa gcaatgccag
     7741 cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat gttcccaacc
     7801 ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa actgccagtg
     7861 cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat tgatcatttt
     7921 ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag cggtgacgtt
     7981 ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca accccagacg
     8041 gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa tatcctgcag
     8101 ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa aagcgttaat
     8161 ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga tgcaatagtg
     8221 aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca gtatactccc
     8281 gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg taaggtattc
     8341 cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt gcggcaggcg
     8401 cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc ggcgactatc
     8461 aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga caattagcag
     8521 agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag atcgccggtc
     8581 caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt cagcaggcgc
     8641 tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg gttgactact
     8701 ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc attattggtg
     8761 acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca aaccacgtcg
     8821 gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag cagcaggaaa
     8881 acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg aaaaagcatt
     8941 acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa ctgcaaagcg
     9001 gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg acgcagaacc
     9061 agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg ggcgaaagcc
     9121 tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt ctggcagtag
     9181 aaagcgaagg ggcgaccgtc gtattccttg atgtgtttaa aaacaaggaa ggcgaaccga
     9241 tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact gatatcgcct
     9301 gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac atcgactccc
     9361 gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc tatgtaccgg
     9421 aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac ggcaaaccgt
     9481 tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa gccctggagc
     9541 gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag ctggaaaaac
     9601 tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa aaccgcacca
     9661 cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc gcgccataca
     9721 tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt aacgaagagc
     9781 aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg gcagctcgcc
     9841 tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg catgtaatgt
     9901 gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac tgcccgatcc
     9961 tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa ctgacagcga
    10021 agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt atgtaatcga
    10081 tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta ttgccatcta
    10141 aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga ggccagaagc
    10201 gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg aagtgtttaa
    10261 atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag atacggttat
    10321 agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt cagatgatga
    10381 tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg tcgatgcgca
    10441 attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac gcacaggtgc
    10501 aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag cctggctgga
    10561 ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg atttaagtga
    10621 cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata cgcgaactcg
    10681 ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga caagaggata
    10741 attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat tgcaacaaaa
    10801 catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt tttgtctttc
    10861 agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt aaccgc
//
LOCUS       DAJTUI010000117        10893 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTUI010000117 DAJTUI010000000
VERSION     DAJTUI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.117"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..242)
                     /locus_tag="OO464_003422"
     CDS             complement(<1..242)
                     /locus_tag="OO464_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCR6228448.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPV"
     gene            complement(404..478)
                     /locus_tag="OO464_003423"
     tRNA            complement(404..478)
                     /locus_tag="OO464_003423"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(443..445),aa:Arg,seq:cct)
     gene            complement(552..1484)
                     /locus_tag="OO464_003424"
                     /pseudo
     CDS             complement(552..1484)
                     /locus_tag="OO464_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1778..2533
                     /gene="mlaA"
                     /locus_tag="OO464_003425"
     CDS             1778..2533
                     /gene="mlaA"
                     /locus_tag="OO464_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR6228449.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2715..3773)
                     /locus_tag="OO464_003426"
     CDS             complement(2715..3773)
                     /locus_tag="OO464_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228450.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4139..5479)
                     /gene="fadL"
                     /locus_tag="OO464_003427"
     CDS             complement(4139..5479)
                     /gene="fadL"
                     /locus_tag="OO464_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6228451.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            5851..6135
                     /locus_tag="OO464_003428"
     CDS             5851..6135
                     /locus_tag="OO464_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6228452.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6315..7625
                     /gene="fadI"
                     /locus_tag="OO464_003429"
     CDS             6315..7625
                     /gene="fadI"
                     /locus_tag="OO464_003429"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6228453.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7625..9769
                     /gene="fadJ"
                     /locus_tag="OO464_003430"
     CDS             7625..9769
                     /gene="fadJ"
                     /locus_tag="OO464_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR6228454.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            9972..10457
                     /gene="sixA"
                     /locus_tag="OO464_003431"
     CDS             9972..10457
                     /gene="sixA"
                     /locus_tag="OO464_003431"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6228455.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2725 a         2488 c         2706 g         2974 t
ORIGIN      
        1 accggatcga tgccagccga gatgtctttc ctcgcagtcc atgctttatc ccttgcctcc
       61 tgcaaagtca taagcggata ttttccgacg gtcaggattt tctccttacc gtcaatcttg
      121 tagcgaagct gccatacctt tttcccggat acagggacat aaaggtacag gccattacca
      181 tcgagaaggc ggtatggttt ttctttcggc tttgctgctt caatctgctt aacggtgagc
      241 atgggtaaaa atccggtggg taaaattatt ttatccactt tttacccgtc atggagtgcg
      301 gctgtcaacg atctgacgcg aaccatgacg aactgtgaat atacggaagg cttgatattc
      361 aggggatttt gcggactggt acggatggga gcgaactgat aaatggtgtc ccctgcagga
      421 atcgaacctg caattagccc ttaggagggg ctcgttatat ccatttaact aagaggacaa
      481 tgcggcgagt atacccgcta atggactgcg gggtaagtac gctgccgctc gattgcttaa
      541 accctcgcca tttatgctgg gtttttatca tttttcttaa tgttttccgc acgttctgct
      601 ttttggcgtg cttctgcttt acgcttgttg ctcatgtcgt tacgaatctg tgcatgactc
      661 attaacgcga agataaaggt gccgccgcag atgttccccg ctaaagtagg tagtgcgaag
      721 ggccagatga aatcgctcca gtgcagcgta ccgttaaaca ccagatagag gatttcaaca
      781 gaaccgacca cgatatgggt ggtgtcaccc agggcaataa gccaggtcat caatataatc
      841 accacaatct ttgccgcacc tgctgcagga aacatccata ccatagtggc gatcagccag
      901 ccggaaatga tcgcgttggc aaacatctcg ctgggggtgt tcttcatcac atccatgccg
      961 attttgacaa atgcatcgcg agtttcttca ttgaagatag gcatatattc aaatgcccac
     1021 gcagcaatac cagtcccgag aatattaccc agcagcacga ctccccataa ccgcataagt
     1081 aagccaacgt tgctcattgt cggtttttgc atgacgggta gtaccgcagt cacggtgttt
     1141 tcggtaaata attgctggcg ggccattatg acgataataa aaccaaaggt ataaccgaga
     1201 ttctccagca agaagctgcc cggcacacct tccagttcgg catgaaatat cccttttgcc
     1261 agtaacgaag cgcccatcga cagacccgcc gcaatggctg accacagtag cgccattgcg
     1321 tcgcgttcta gctctttttc accatcctgg cggatatgct catgaattgc catcgctcgg
     1381 gaggggagtc ggtcttcatc tatttctatt tttttgccgc gctctttttc ttcgctctaa
     1441 acttcaattt cgtcgctgtg ttgatcaatt ttgtcgttgt ccatggtctc ttctcttgaa
     1501 ttagcacgta tagctaaagc gtagcggctt ttttgctcgc aactggcggg agttactctg
     1561 aaaatgtaga aaaggctgcg tttgcctttt tctgtttcta tagaatcaag tagcctacag
     1621 ggcggcgatt accaggctat gatcaaatca gcaaatcagg gcgtctggac atcagttgac
     1681 gtgctgttac aatcgcccac acctaaacag gcggatacgg tatcgttccg tcatggatgg
     1741 caaactgcat aagccataaa aaaacaggga gacatttatg aagcttcgcc tgtcggcgct
     1801 tgctctggga actacgcttc tggtggggtg tgcgagttcc ggtacagatc agcaagggcg
     1861 ttctgacccg ttagaagggt tcaaccgcac catgtacaac ttcaacttca acgtattaga
     1921 cccgtatatt gttcgaccgg tcgctgtcgc ctggcgtgat tatgttccgc aaccggcgcg
     1981 taatggtttg agcaacttta ctggcaacct tgaagaacct gcggtgatgg ttaactactt
     2041 cttgcagggc gacccttatc aggggatggt ccactttacc cgctttttcc tgaacaccat
     2101 tttggggatg ggcggcttta ttgatgttgc agggatggcg aacccgaaac tgcaacggac
     2161 tgaacctcac cgcttcggta gtacgcttgg tcattatggc gtgggttatg ggccttacgt
     2221 tcagttaccg ttctacggta gcttcacgct gcgtgatgac ggtggtgata tggcggatgc
     2281 tctttacccg gttctttcct ggctgacctg gccgatgtct gtgggtaaat ggacgcttga
     2341 agggatcgaa acccgcgctc agctgctgga ttccgatggt ctgctgcgtc agtcgtccga
     2401 tccttatatt atggtgcgcg aagcgtactt ccagcgtcat gatttcatcg ctaatggcgg
     2461 cgaactcaaa ccgcaggaaa atccgaacgc gcaagcgatt caggatgatt taaaagatat
     2521 cgattctgaa taagaaacaa ataaaaaagg tgagtcgcaa aactcacctt ttttgttgtt
     2581 atccctcaat taccacaagt aaaaggagat aaaccgacaa tgacccggac gtaatgatgt
     2641 aaaaatactg atgtttgtca ctgactattt ccgatattca ggcgttgaaa aatcaaaaaa
     2701 ctggaaaaat aattttaaat atcatcaaaa ttaacaccta gtgcagatag ttgattaatt
     2761 tcattttgat atttcgtctg gcgattttga agtctttttt caactttatc ttcgagattc
     2821 ttaaagtctt tgtatttatt ttcaggatgg tcaacgtcaa ctttgttagc gaataaagta
     2881 gaaattattt cttttacatt atcgcgtcta aattttgcat cgtccacttg aaaattatat
     2941 cttattgctc gacctaaacg tttaaaaact tcgttaggtt ctctcagtct ttccccgttg
     3001 agctcgttta caacctgatg atcggaacca gttgcaaatg attctgccat gatagcttta
     3061 ctgctttcaa accacgctga agcactcctg gcgttttcac tacaatattg ggtgagacta
     3121 tcaccattaa tacgaattcc tctttgaatg tggtagtcgt cctgatcgta gtaaccgaga
     3181 gataagtaat catttccgtt tttattttta attacaatag cgagagagtc gcctttttta
     3241 tctttaccgt accaggtaaa taagtcagtt ggttcgcttt tcccgctctc atcaaattcc
     3301 gttaccgtat gtctgagttt gtgtgatatc ttatcgaaga taaaaccagg atctccatca
     3361 tatatttctt ttaatgcgct gaagatcatt tccttgttat tgatatcatg agtcttctca
     3421 actatgttgt cattaatgat agtacggacg agttctttgt ctttatagtt cgcaggtaat
     3481 tctattggtt cttggttgtc gataccaata gtaatatggt ctgtatcatt ggagccaata
     3541 tcaatataag taactgagga attattaata ttagatctat aaggatcgtc gtttactcgt
     3601 atggcgtttt ttataagctc aagtagatgt ttaatacagg tgcttattcc ttccttacca
     3661 ttaaaaaggt ttttgaactc ttgtactaat ccattaattt catcattgtt attttcattg
     3721 atggattctg ggtaagctgc gctggtattg ttgattgaaa tagatggtag catgatttta
     3781 ttccatgttg attgatttgt tacaaatata tattacctaa tgaaatagac tatcaaaaaa
     3841 cgctcattta tttagaacct atctttaatt tgagtgtata agcgcacaat agccaggggg
     3901 gttgagtatc ctggaaatgc agaaaagtaa actctttgag aaggacgagc agaacgcttg
     3961 aaaatatgca tttcccttca ggtttgtctg tggataccac ttactgattt gaaagggcaa
     4021 gctgccatca ggctgtttgc aaaactcagg aggcaagcaa tgaggaaact actttcggtg
     4081 aagtggggga gttaaagtca cctgcatagc aggtgacttt atccaggcga acgcgttatc
     4141 agaacgcgta gttaaagtta gtaccgaaca gccaggcttt accttcagac tcgaactggt
     4201 atgggccttc gttaattttc acgctctgac cgtgcatata agaaacacca acgtcgactg
     4261 aagcatcttt attaaatgcg taagtcgtac ctgcactcag ccagaaacgg tcctggtccg
     4321 gaatggagat agaacgattc tgagccggaa ctgggctgtc atcaaaggcg ataccggtac
     4381 ggaaggtcca gttatcatcg tagtaataag tggtacccaa cgcgatgcgg taagcatctt
     4441 taaagccttc atgtttctgg aacagcgtgt cgccactggt tgaggtcgct ttcagctgct
     4501 ggaactgact ccagctggtg taagccaggc tatagtgaat cgcccactgt ggatcaacac
     4561 ggttataacc tgacacttcc cacatttcag gcaggttcag cgtcagataa cccgattgcg
     4621 ttgcgccacc tgtcgcggta ggaattggca aaccgtagtt attaaacgca cgattaagat
     4681 cgctgctgta gttacctttg aagtcaattt tcacttcaga acggtaggtc agtgcatagc
     4741 ggttattttt atccagttca tacaggattc cggcgttcca gccaaagccc cactggttac
     4801 cgttcagatg agcgattttg gtattactgt cgataccgtt ggcggtagct gccaatgctt
     4861 gcccttgagg agtttgcccg gcaggagatt gcataatctg accagcaacc aactgcccca
     4921 gatcgcctgc gaaacgttca attttcgcgc gagcgtagat ggcgttgaaa ccaagaccaa
     4981 agctccatgc attatttaag cgatacgcac cgcttaagtt caggttcatg gtttcaaggt
     5041 cagttgtacc cccgacagag ccgcctgcat aagtatcatt aaactctgtt gccagaccat
     5101 agttagaggt aatagaagcg ccccaaccaa attggtcgtt aatcggtgca acaaagtgca
     5161 tgttcggaac ccatgccgta ggcgcgatgt tatcggcttt caggctacga ccagacggag
     5221 acgttccgct gatattcaca tccgggtcaa tataaaccgc acccgcagaa aatgtcgggc
     5281 ggtcaaacat ggtaatcaat gcggggttac ggctaacgtt acctgcatca tcggcaattg
     5341 cgccttcccc tgaataagcc cggcccaggc cagaggaaga aaattcgttt aactgaaagc
     5401 ctgccgacca ggcctgggtg gagataagtg ccactgcgac tgcgagagca gactttgtaa
     5461 acagggtttt ctggctcatg accataacct caatgattta tttttataca aactatgtta
     5521 cgtgctgtaa caggagcgcg aagtgtaggg tctgaggtac atcttagaaa tcagaccagt
     5581 ggcgagagta taggtcggac cagctagaat gttgcaagta tgtttccatc ttttttcaaa
     5641 tatgatctaa gaaacgcgat ccagctggca aaatttggag gttgcgcact cgcggattcg
     5701 ggtgaatttt gttttagatc atttttaagt gtgatttcgg tcacttatcc gattttgcag
     5761 aattaacaac tggagcagca ctttccggaa gcgtaaaata tccacaacgt ttatctggca
     5821 ccttgggtgc acatacagag gaaatcaacg atgagtaaat gtagtgctga tgaaaccccg
     5881 gtttgctgct gtatggatgt tggcaccatt atggacaact ccgactgcac cgcgtcttac
     5941 agccgcgtgt ttgcaaaccg cgcagaagca gaacaaacgc tggcggcgct gactgaaaaa
     6001 tcccgtagcg tgaaatccga accgtgcaaa atctccccga ctttcactga agagtctgac
     6061 ggtgtgcgcc tggatatcga tttcactttt gcctgcgaag ctgaaatgct gatcttccag
     6121 ctgggtctgc gttaattcct ctcgggtacg tacgcccctg tcagttgctg gcaggggcgt
     6181 tttcttttct ttcttctgtc ttcactgtgt catagctccc actttccttt ttctccgctt
     6241 ggctaaatgt aaaaaaatgg ttaagactat gatcaggtca gaccacttta tttatttttt
     6301 tacaggggag tgttatgggt caggttttac cactggttac ccgccagggc gatcgtatcg
     6361 ccattgttag cggtttacgt acgccttttg cccgccaggc gacggctttt catggcattc
     6421 ccacggttga tttagggaag atggtggtag gcgaactgct ggcacgcagc gagatccccg
     6481 ctgaagtgat tgaacagctg gtctttggtc aggtcgtaca aatgcctgaa gcccccaaca
     6541 ttgcgcgtga aattgttctc ggtaccggaa tgaatgtgca taccgatgct tacagcgtca
     6601 gccgcgcttg cgctaccagt ttccaagcgg ttgcaaacgt cgcagaaagc ctgatggcgg
     6661 gaactatccg agcggggatt gccggtgggg cagattcctc ttccgtattg ccaattggcg
     6721 tcagtaaaaa actggcgtac gtgctggttg atgtcaacaa agctcgaacc atgagccagc
     6781 gcctgaaact cttctctcgc ctgcgtttgc gcgacttaat gcccgtaccg cctgcggtag
     6841 cagaatattc tactggcttg cggatgggcg acaccgccga acaaatggcg aaaacctacg
     6901 gcatcacccg agaacagcaa gatgcattag cgcaccgttc gcatcagcgt gccgctcagg
     6961 catggtcaga cggaaaactc aaagaagagg tgatgactgc ctttatccct ccttataaac
     7021 aaccgcttgt cgaagacaac aatattcgcg gtaattcctc gcttgccgat tacgcaaagc
     7081 tgcgcccggc gtttgatcgc aaacacggaa cggtaacggc ggcaaacagt acgccgctga
     7141 ccgatggcgc ggcagcggtg atcctgatga ctgaatcccg ggcgaaagaa ttagggctgg
     7201 tgccgctggg gtatctgcgc agctacgcat ttactgcgat tgatgtctgg caggacatgt
     7261 tgctcggtcc agcctggtca acaccgctgg cgctggaacg tgccggtttg acgatgagcg
     7321 aactgacatt gatcgatatg cacgaagcct ttgcagctca gacactggcg aatattcagt
     7381 tgctgggtag tgaacgtttt gctcgtgaag tactggggcg tgcacatgcc actggcgaag
     7441 tggacgatag caaatttaac gtgcttggcg gttcgattgc ttacggacat cccttcgcgg
     7501 cgaccggcgc gcggatgatt acccagacat tgcatgaact tcgccgtcgc ggcggtggat
     7561 ttggtttagt taccgcctgt gctgccggtg ggcttggcgc ggcaatggtt ctggaggcgg
     7621 aataatggaa atggcatcag cgtttactct taatgttcgt ctggacaaca ttgctatcat
     7681 caccatcgac gtaccggatg agaaaatgaa taccctgaag gcggaatttg cctcgcaggt
     7741 gcgcgccatt attaagcaac tccgtgaaaa caaagagttg cgcggcgtgg tgtttatctc
     7801 cgcgaaaccg gacaatttta tcgccggagc agacatcaac atgattggca actgcaaaac
     7861 ggcgcaagaa gcggaagcac tggcgcggca gggccaacag ttgatggcgg agattcatgc
     7921 tttgcccatt ccggttatcg cggctattca tggcgcttgc ctgggcggtg ggctggagct
     7981 ggcgctggcg tgccacggtc gcgtttgtac tgacgatcct aaaacggtgc tcggtttgcc
     8041 tgaagtacaa cttggattgt tacccggttc aggcggcacc cagcgtttac cgcgtctgat
     8101 aggcgtcagt acagcattag agatgattct caccggaaaa caacttcggg cgaaacaggc
     8161 attaaagctg gggctggtgg atgacgttgt tccgcactcc attttgctgg aagccgctgt
     8221 tgagctggcg aagaaggatc gcccatcttc ccgccctcta cctgtacgcg agcgtattct
     8281 ggcggggccg ttaggtcgtg cgctgctgtt caaaatggtc ggcaagaaaa cagaacacaa
     8341 aactcaaggc aattatccgg cgacagaacg catcctggag gttgttgaaa cgggattagc
     8401 gcagggcacc agcagcggct atgacgctga agctcgcgcc tttggcgaac tggcgatgac
     8461 gccgcaatcg caagctttgc gtagtatctt ttttgccagt acggacgtga agaaagatcc
     8521 cggtagtgat gcaccgcctg cgccattaaa cagcgtggga attttaggtg gtggcttgat
     8581 gggcggcggt attgcttatg tcactgcttg taaagcgggg cttccggtca gaattaaaga
     8641 tatcaacccg cagggcataa atcatgcgct gaagtacagt tgggatcagc tggagggcaa
     8701 agttcgccgt cgtcatctca aagccagcga acgtgacaaa cagctggcat taatctccgg
     8761 aacgacggac tatcgcggct ttgcccatcg cgatctgatt attgaagcgg tgtttgaaaa
     8821 tctcgaatta aaacaacaga tggtggcgga agtggagcaa aattgcgccg ctcataccat
     8881 ttttgcttcg aacacgtcat ctttaccgat tggtgatatc gccgctcacg ccgcgcgacc
     8941 tgagcaagtt attggcctgc atttcttcag tccggtggaa aaaatgccgc tggtagagat
     9001 tattcctcat gcgggtacat cggcgcaaac catcgccact acggtaaaac tggcgaaaaa
     9061 acagggcaaa acgcccattg tcgtgcgtga caaagccggg ttttacgtca atcgcatctt
     9121 agcgccttac attaatgaag ctatccgcat gctgaccgaa ggtgaacggg tagagcacat
     9181 tgatgccgcg ctagtgaaat ttggttttcc ggtaggccca atccaacttt tggatgaggt
     9241 aggaatcgac accgggacta aaattattcc tgtactggaa gccgcttatg gagaacgttt
     9301 tagcgcgcct gcaaatgttg tttcttcaat tttgaacgac gatcgcaaag gcagaaaaaa
     9361 tggtcggggt ttctatcttt atggtcagaa agggcgtaaa agcaaaaaac aggtcgatcc
     9421 tgccatttac ccgctcattg gcgcacaagg gcaggggcga ctctccgcac cgcaggttgc
     9481 tgaacggtgt gtgatgttga tgctgaatga agcagtacgt tgtgttgatg agcaggttat
     9541 ccgtagcgtg cgtgacgggg atattggtgc ggtatttggc attggttttc cgccatttct
     9601 cggtggaccg ttccgctata tcgattctct cggcgcgggc gaagtggttg caataatgca
     9661 acgactggcc acgcagtatg gttcccgttt taccccttgc gagcgtttgg tcgagatggg
     9721 cgcgcgtggg gaaagttttt ggaaaacaac tgcaactgac ctgcaataag aaggtcaaag
     9781 ctatatgaat ccgcgctgaa tggcggagtg ttggtcaaaa tgtaaacgca tattgactat
     9841 acttacgcca ttgaggtaaa aaacagcgtt tcattcggtg aatggataag gcacaatgcc
     9901 ggccaccgtt ttctttctct ggtttcagat gaaagaaaac gggcgaatct ggttaacaaa
     9961 agcggtgcaa tatgcaagtt tttatcatgc gtcacggcga cgcagccctc gattccgcca
    10021 gtgattcggt tcgtcctctg accactaatg gttgtgacga atctcgcctg atggcgaact
    10081 ggctgaaagg tcaaaaagtg gaaatcgaac gcgttctggt gagcccgttc ctgcgagccg
    10141 agcaaacact ggaagaagta ggggattgtc tgaacctgcc ttccagtgca gaagttctgc
    10201 cggagttaac gccctgcggc gatgttggtc tggtcagtgc ctatttgcag gcgttgacca
    10261 atgaaggtgt tgcttcggta ctggtgatct ctcacttacc gttagtcggg tatctggttg
    10321 ccgagttatg tccgggcgaa acgccgccta tgtttaccac ctcggccatt gccagcgtaa
    10381 cgcttgatga aagcggaaac ggtacattca actggcagat gagcccgtgt aatctgaaga
    10441 tggcaaaagc tatctgatgt gttccaaatt atgcccaatg aggtagattc attgggcata
    10501 attgctcatt aggcagcgga tatctgtcaa cctcactact atcgaagact ctccttatct
    10561 ataaataaaa cactttctac attcattcgg aaattattct tctacgcgta tcttgatttt
    10621 gcccaaagtt acatgttgaa atgaataata tctgtttgac actcattgtc atttattaat
    10681 acagataata gagaaaatag agttgaataa tcaggcagga tgtaattgaa acttgattaa
    10741 attctggcta tagtgtttat atattatatt gtcaaaagtg gataaatgcc aatcgcatta
    10801 tatgttcatt gtttgttttt taagctgttg atattcatta ttgatatact ttctattttt
    10861 tctaaatact tattccgtat tttttaagga tgt
//
LOCUS       DAJTUI010000118        10800 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTUI010000118 DAJTUI010000000
VERSION     DAJTUI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.118"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..154)
                     /locus_tag="OO464_003432"
     CDS             complement(<1..154)
                     /locus_tag="OO464_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6228456.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHV"
     gene            complement(266..1294)
                     /gene="malI"
                     /locus_tag="OO464_003433"
     CDS             complement(266..1294)
                     /gene="malI"
                     /locus_tag="OO464_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6228457.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1469..3061
                     /gene="malX"
                     /locus_tag="OO464_003434"
     CDS             1469..3061
                     /gene="malX"
                     /locus_tag="OO464_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6228458.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3071..4243
                     /gene="malY"
                     /locus_tag="OO464_003435"
     CDS             3071..4243
                     /gene="malY"
                     /locus_tag="OO464_003435"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6228459.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4347..5348
                     /gene="add"
                     /locus_tag="OO464_003436"
     CDS             4347..5348
                     /gene="add"
                     /locus_tag="OO464_003436"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6228460.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5384..6424)
                     /locus_tag="OO464_003437"
     CDS             complement(5384..6424)
                     /locus_tag="OO464_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6228461.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6667..6792
                     /gene="blr"
                     /locus_tag="OO464_003438"
     CDS             6667..6792
                     /gene="blr"
                     /locus_tag="OO464_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6228462.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7065..7280
                     /gene="ydgT"
                     /locus_tag="OO464_003439"
     CDS             7065..7280
                     /gene="ydgT"
                     /locus_tag="OO464_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6228463.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7366..7806
                     /locus_tag="OO464_003440"
     CDS             7366..7806
                     /locus_tag="OO464_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6228464.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7883..8464
                     /gene="rsxA"
                     /locus_tag="OO464_003441"
     CDS             7883..8464
                     /gene="rsxA"
                     /locus_tag="OO464_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6228465.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8464..9042
                     /gene="rsxB"
                     /locus_tag="OO464_003442"
     CDS             8464..9042
                     /gene="rsxB"
                     /locus_tag="OO464_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6228466.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9035..>10800
                     /gene="rsxC"
                     /locus_tag="OO464_003443"
     CDS             9035..>10800
                     /gene="rsxC"
                     /locus_tag="OO464_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6228467.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKA"
BASE COUNT         2495 a         2791 c         2742 g         2772 t
ORIGIN      
        1 caacatggtt agctgcgtcg gcgttaatat cactgaccac cacagatgcg ccagctgtcg
       61 cgaatgtaat ggcgatttct ttaccaatac ctgcacccgc acctgtgatg atggcgcatt
      121 ttccgtcgag tctcaggttg tcagaattaa acacataaac ctcctgtgaa cttcagtaaa
      181 aataaaaggc accgcctgtg tgccacattt tttatcatta accttgagga aaggacacta
      241 ccaagtatag cagataaaga gaggattatt tcgccgcaat gagccgggcg ggaataataa
      301 gattgcgtga atgcgtctct tcatgggtga ttttttgcat catgcgatcc gcaagtgtga
      361 taccaagttc ccgcgctggc gtgctggccc aggtaacagg tatatcatca agtgtggttg
      421 gtgtcgcatc ggtaaatgcc gccagcgaaa cctgttgctc aaagtaacga tcgactccgc
      481 tttccccgct ttgccgccct gctttcagca aaccaaacca tgcccccatc gcaatagttt
      541 cgttatagca aaccacggca ctgatggtcg ggttatgacg taaaagcgcc gtgatagctt
      601 ccgcggcttg cttctggctg gaagtgcact ccaacaccca atcgctgtga aacggcaggc
      661 caaattttag tagagttgca caatagcccc ccacccgttc tgcacgggtt aatgaggaac
      721 tttgccctcc cagccaggcg atccgctgat gcccattgcg aatgagatgc tccgtcaaca
      781 actgtgcagc ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga taactggcac
      841 gggaagcgaa aatcaccggg atagcttttt cttctgccat ccgccgcagg tcatcgctgc
      901 ttcctgcagc cccggcaatt accacaccat cgacaccctg attcagtaac agtgaaaacc
      961 gctgtgccag ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc cgtccctgtg
     1021 cttccagagc ttccgtcaat ccggccgtca attcggcgta aaacggcgca gataaatcac
     1081 ggacgatcaa accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc tggcgattgc
     1141 gcacaaatcc cagctcttca atggcggcgt taacgcgttc tcctgtggcg gtagagattc
     1201 gccctttgcc attaagcacc agcgaaacgg tacttaccga cacgcccgca gccagcgcaa
     1261 catcatgaat ggttattttt ttggcggtag ccatgcagat gacctactcc ctgattatgt
     1321 gacagataaa acgttttacc ttttatttat cttatacccg ctattatcgt tgcgtaatgt
     1381 gatttatgac tcactaaaat ttgataaaac gttttatctt ctcgcgcaat ttactgaatc
     1441 cagattgttc tctacgagga gtcgttttat gacggcgaaa acagcaccga aagtcacgct
     1501 gtgggagttc ttccagcagt taggcaaaac cttcatgtta cccgtggcat tattgtcgtt
     1561 ctgcggcatt atgctcggca ttggtagttc tcttagcagc catgatgtca taaccctgat
     1621 cccggtcctg ggcaaccccg tgttgcaggc tatctttacc tggatgagta agattggctc
     1681 gtttgctttt agtttcctgc ctgtcatgtt ctgtatcgcc atcccgctgg gcctggcacg
     1741 cgaaaataaa ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa tgaacctcgc
     1801 ggtaaacttc tggttgacca ataaaggcat tctgccaacc acagatgccg ccgttctgaa
     1861 agccaataac atccagagca ttcttgggat ccagtcgatc gataccggga tcctcggtgc
     1921 ggtgatcgcc ggtattatcg tctggatgct gcatgagcgt ttccataata tccgcctgcc
     1981 ggatgcgctg gcattcttcg gcggtacgcg cttcgtacca attatctcct cgctggtgat
     2041 gggccttgtc ggcctggtga ttccattagt ctggccgatt ttcgccatgg gtattagcgg
     2101 cttgggccat atgataaaca gcgcgggtga tttcggaccg atgctgtttg gtaccggtga
     2161 acgtctgctg ttgccgtttg gtctgcatca cattctggtg gcattaattc gctttaccga
     2221 cgcaggcggc acgcaggagg tctgcggtca aaccgtcagc ggcgcactga ccatcttcca
     2281 ggcgcaattg agttgcccga ccactcacgg tttttctgaa agcgtcacgc gtttcctttc
     2341 gcaaggtaaa atgcctgcgt ttctcggcgg tctaccaggt gcagcgttag ctatgtatca
     2401 ctgcgcgcgc ccggaaaatc gccataaaat taaaggtctg ctgatttctg gcctgatcgc
     2461 ctgcgtcgtt ggcggcacta ccgaaccgct ggaattcctg ttcctgttcg tagcgccagt
     2521 tctgtatgtc atccacgcgc tgttaaccgg cctcggcttc accgtcatgt ctgtgctcgg
     2581 cgtcaccatc ggtaataccg acggcaatat catcgacttc gtggtgttcg gtattttgca
     2641 tggtctgtca accaagtggt acatggtgcc agtggtggcg gcaatctggt ttgtcgttta
     2701 ctacgtcatc ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg ggcgcgatag
     2761 cgaagttgcc agctcaatcg aaaaagccgt tgccggtgcg ccgggtaaat caggttacaa
     2821 cgttcctgca atcctcgaag cattaggcgg tgccgacaat attgtcagcc tcgataactg
     2881 cattacccgt ctgcgtttgt ctgtggaaga tatgtcgctt gttaatgtgc aggcactgaa
     2941 ggacaatcgg gcaattggcg tagtacaact taatcaacat aacctgcagg ttgttatcgg
     3001 gccacaagtt cagtcagtaa aagatgaaat ggccggtctg atgcatactg tccaggcata
     3061 aggataagat atgttcgatt tttcaaaggt cgtggatcgt catggcacat ggtgtacaca
     3121 gtgggattat gtcgctgacc gtttcggcac tgctgacctg ttaccgttca cgatttcaga
     3181 catggatttt gccactgccc cctgcattat cgaggcgctg aatcagcgcc tgatgcacgg
     3241 cgtatttggc tacagccgct ggaaaaacga tgagtttctc gcggctattg cccactggtt
     3301 ttccacccag cattacaccg caattgatac gcagacggtg gtgtatggcc cttctgtcat
     3361 ctatatggtt tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg tggtgatcca
     3421 cacgcccgcc tatgacgcat tttacaaggc cattgaaggt aaccagcgca cagtaatgcc
     3481 cgttgcttta gagaagcagg ctgacggctg gttttgcgat atgggcaagc tggaagccgt
     3541 tttggcgaaa ccagaatgta aaattatgct cctgtgtaac ccacagaatc ctaccgggaa
     3601 agtgtggacg tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc atgatgtgcg
     3661 ggttatttcc gatgaaatcc atatggatat ggtctggggc gagcagccgc atattccctg
     3721 gagtaatgtg gcgcggggag actgggcgtt gctgacatcg ggatcgaaaa gtttcaatat
     3781 tcccgcgctg accggtgctt acgggattat agaaaatagc agcagccgtg atgcctattt
     3841 atcggcactg aaaggacgtg atgggctttc ttccccttcg gtactggcgt taactgccca
     3901 tatcgccgcc tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct atctgaaaga
     3961 taacctgacg tatatctcag ataaaatgaa cgccgcgttt cctgaactca actggcagat
     4021 cccgcaatcc acttatctgg cctggcttga tctacgtccg ttgaatattg acgacaacgc
     4081 gttgcaaaaa gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt atacctatgg
     4141 tgaagaaggt cgtggttttg tccgtctcaa tgccggctgc ccacgttcga aactggaaaa
     4201 aggtgtggct ggattaataa acgccatccg cgctgttcgc taaccccaat tgcgcaacgt
     4261 aaaaaatcgt tgcgcaatcg tggattttta ccctgctttg tttttataat ggtgcgcact
     4321 tttatatcca gaaaaagagt gcgaccatga ttgataccac cctgccatta actgatatcc
     4381 atcgccacct tgatggcaac attcgtcccc agaccattct tgaacttggc cgccagtata
     4441 atatctcgct tcctgcacaa tcccttgaaa cactgattcc ccacgttcag gtcattgcca
     4501 acgaacccga tctggtgagc tttctgacca aacttgactg gggcgttaaa gttctcgcct
     4561 ctcttgatgc ctgccgccgc gtggcatttg aaaacattga agatgcagcc cgtaacggcc
     4621 tgcgctatgt cgagctgcgt ttttcaccag gctacatggc aatggcacat aagctgcctg
     4681 tagcgggtgt tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc acctttggtg
     4741 tgcaggcgaa gcttatcggc attatgagcc gaaccttcgg cgaagccgcc tgtcagcaag
     4801 agctggaggc ctttttagcc caccgtgacc agattaccgc acttgattta gctggtgatg
     4861 aacttggatt cccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt gatgcgggct
     4921 ggcatattac cgtccatgca ggcgaagctg ccgggccgga aagcatctgg caggcgattc
     4981 gtgaactggg tgcggagcgt attggacatg gcgtaaaagc cattgaagat cgggcgctga
     5041 tggattttct cgccgagcaa caaattggta ttgaatcctg tctgacctcc aatattcaga
     5101 ccagcaccgt agcagagctg gctgcacatc cgctgaaaac gttccttgag catggcattc
     5161 gtgccagcat taacactgac gatcccggcg tacagggagt ggatatcatt cacgaatata
     5221 ccgttgccgc gccagctgct gggttatccc gtgagcaaat ccgccaggca cagattaatg
     5281 gtctggaaat ggctttcctc agcgcagagg aaaaacgcgc actgcgagaa aaagtcgccg
     5341 cgaagtaaca aaaatggatg gtgcaaattg caccatccat ttttcacgca aggcacaacg
     5401 tcgcgcgatg tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct
     5461 ggcttgccgg aaccggattt tcaccatcgc catttaacgc atcacgaatc gccgcatagt
     5521 aagccggata attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca
     5581 cgcgggtaag tacgccatca cgcatatcgt agccccagtc ttcctgcggc agacgctcgc
     5641 catttttcag acgttcttcc tgtggatcga ggccatattt cacataactg cctcgggatc
     5701 catgcacgat ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc
     5761 gccgctgtgg ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta
     5821 actgtgccaa atctaccgtc atgctgaccg gtaaaccaaa tagcgtaatg gcctgatcaa
     5881 gaagatgcgg tgctaaatcg taccagatac cgctgcctgg gccgccctgt tcacgccaac
     5941 gatcgcgcac ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca
     6001 gcacgccttc cgcgagcaaa ccttttagcg tcaagaaatc gctatcccaa cggcggttat
     6061 ggaatacaga cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg
     6121 acagtgtcac ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt
     6181 tggctaacgg gaaatgggta tcgttgggtg taggaatgac aatcaggtct atgttgggat
     6241 cgttaaacag atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg gcttttactt
     6301 ttgtttcatc actgctggag attaccgcca gttccagccc gggcgtgccc gcaatcaggg
     6361 gcgcatggaa ggttttgctc gcataaccat acccaatcaa cccaacacgg atgttgtcgc
     6421 tcatgtcatt gcctctcatt gaagtatgat ggctatttga cactatcctt tacccacgct
     6481 caacagttta ataacctgcc agcaataagg gatgttgttt aacttaagtc aaaaaaatag
     6541 cgaattttcc aacgacaaaa gctaaatatc gcaaaaacct cagtaaaaat cttgctggag
     6601 ctattattgc taagtaacat ttaccccctg aagttaatgg atcaatcaag agagatgtgg
     6661 gctgtaatga atcgtcttat tgaattaaca ggttggatcg ttcttgtcgt ttcagtcatt
     6721 cttcttggcg tggcgagtca cattgacaac tatcagccac ctgaacagag tgcttcggta
     6781 caacacaagt aagctctgca cttgtggagc gacatgctgc ccgtccgggt gcatgttttc
     6841 acttgtcgga tattaaacca ggaatttatt atcttgttcg atgttgttgg tgattgtcag
     6901 ggatagtaaa gcgcgttact cttttcagag cagtttttca tccctgaata tttgccatca
     6961 gcgcattctt attattgtaa taacacaagc gttcactttt ggtgaaattt tatcatcact
     7021 aataatacct acccctttgg tattaattat tttatggacc ttttatgact gttcaggact
     7081 acttattaaa atttcgcaaa atcagttcac tcgaaagtct ggaaaaactc tacgaccatc
     7141 ttaattacac cctgacggac gatcaggaac tgatcaatat gtatcgtgct gccgatcacc
     7201 gtcgcgcaga gctggtttct ggcgggcgtt tgtttgacct cggccaggta ccgaagtccg
     7261 tctggcacta tgtccaataa agaaagtagc gatcggttat aaaagcctta taataacgcc
     7321 cctaaaatta gatttgcctg tttgcgtcgc tattctggag aacctatgac caccacaacg
     7381 ccacaacgta ttggaggctg gttgcttggc ccgttggcct ggcttttagt cgctctgttg
     7441 agtacgacgc tggcgttgtt gctgtatacc gctgcgttat cttctccgca aacatttcaa
     7501 acgctcggcg gacaagcact gacgacacaa atcttatggg gcgtttcttt tattaccgct
     7561 atcgccatgt ggtattacac actgtggttg accatcgctt tctttaaacg tcggcgttgt
     7621 gttcctaaac actatattat ctggctgctt atttccgtat tgctggcagt aaaagcattc
     7681 gctttttcac ccgtggaagt tggcatagcc gttcgccagt tgctgtttac tttgttggca
     7741 accgcattga tcgtccccta tttcaaacgc tcgtcgcggg taaaagcgac gtttgtgaat
     7801 ccgtaataac cttacagtta acctgttgtc gcctgctctg gattaacgga taataggcgg
     7861 cttttttatt tcaggccgaa aaatgactga ctacctgtta ctgtttgtcg gaactgtact
     7921 ggtcaataac tttgtactgg tcaagtttct cggtctctgt ccgtttatgg gggtttccaa
     7981 aaaactggaa accgcgatgg gcatggggct ggcaacaacg tttgtgatga cgctgacgtc
     8041 tatttgcgcc tggcttatcg atacgtggat tttgatccca cttaatctga tttacctgcg
     8101 caccctggca tttattctgg tgattgctgt ggtcgtgcag ttcaccgaga tggtggtgcg
     8161 caaaaccagc ccggtgcttt accgtctgct ggggattttt ttgccgctta tcaccaccaa
     8221 ctgtgcagtg ctcggcgtgg cgttgctgaa tatcaatctc gggcacaatt tcttgcagtc
     8281 ggcgctgtac ggtttttccg ccgctgtcgg tttctcgctg gtgatggtgc tcttcgccgc
     8341 catccgcgaa cgccttgctg tggctgatgt cccggcacct tttcgcggta atgccattgc
     8401 gttaattacc gcaggtctta tgtctctggc ctttatgggc tttagtggtt tggtgaagtt
     8461 gtaatgaatg ctatctggat tgccgttgcc gccgtgagcc tgctgggcct ggcgtttggc
     8521 gccattctgg gttatgcctc ccgccgtttt gcggtggaag acgatccggt cgttgagaaa
     8581 attgacgaaa tcttaccgca gagccagtgt ggtcagtgcg gttatcccgg ctgtcgcccc
     8641 tacgcggaag ccatcagctg taacggtgaa aaaatcaacc gttgcgcccc aggtggcgaa
     8701 gctgtgatgc taaaaattgc cgagttgctt aatgtcgagc cgcagccgct ggatggcgaa
     8761 gcgcaagagc taacgcctgc gcggatggtg gcggttattg atgaaaataa ctgtattggc
     8821 tgcactaaat gtattcaggc ttgtccggta gacgccatcg ttggcgctac ccgtgtcatg
     8881 catacggtaa tgagtgatct ctgtacgggc tgcaatttat gtgttgatcc gtgcccgacg
     8941 cactgcatct cgttgcaacc ggtcgcagaa acacctgact cctggaaatg ggatctgaac
     9001 accattcccg tgcgtatcat tcccgtggaa caccatgctt aagttattct ctgcattcag
     9061 aaaaaataaa atctgggatt tcaacggcgg catccatcca ccggagatga aaacccagac
     9121 caacggtacc cccctgcgcc aggtactact ggcgcagcat tttgttattc cactgaaaca
     9181 gcatattggc gctgaaggtg agttgtgcgt tagcgtcggc gataaagtat tgcgcggcca
     9241 gccgcttacc cgtggtcgcg gcaaaatgct gcctgttcac gcgcccacct cgggtaccgt
     9301 tacggctatt gcgccccact ctacggctca tccttcagct ttagctgaat taagcgtgat
     9361 tattgatgcc gatggtgaag actgctggat cccgcgcgac ggctgggtcg attatcgctc
     9421 tcgcagtcgc gaagagttaa tcgagcgcat acatcagttt ggtgttgccg ggctgggcgg
     9481 tgccggattc ccgacaggcg ttaaattgca gggtggtgga gataagattg aaacgttgat
     9541 tatcaacgct gctgagtgcg agccgtacat taccgccgat gaccgtttga tgcaggattg
     9601 cgcggctcag gtcgtagagg gtattcgcat tcttgcgcat attctgcagc cacgcgaaat
     9661 tcttatcggc attgaagata acaaaccgca ggcgatttcc atgctgcgcg cggtgctggc
     9721 ggactctaac gatatttctc tgcgggtgat tccaaccaaa tatccttctg gcggtgctaa
     9781 acaattaacc tacattctga ccgggaagca ggttccacat ggcgggcgtt catccgatat
     9841 cggcgtatta atgcaaaacg tcggcactgc ttatgcagtg aaacgtgccg ttattgatgg
     9901 cgagccgatt accgagcgtg ttgtaaccct gactggcgaa gcaatcgctc gcccgggcaa
     9961 cgtctgggca cggctgggga cgccagtgcg tcatttattg aatgatgccg gattctgccc
    10021 ctctgccgat caaatggtga ttatgggtgg cccgctaatg ggctttacct tgccatggct
    10081 ggatgtcccg gtcgtaaaga ttaccaactg tctgttggct ccctctgcca atgaacttgg
    10141 cgaaccacag gaagaacaaa gctgcatccg gtgtagcgcc tgtgctgacg cctgccctgt
    10201 ggatcttttg ccgcaacagt tgtactggtt cagcaaaggt cagcaacacg ataaagctac
    10261 cacgcataac attgccgact gcattgaatg tggggcttgc gcgtgggttt gcccgagcaa
    10321 tattcccttg gtgcaatatt tccgtcagga aaaagctgaa attgcggcta ttcgtcagga
    10381 agaaaagcgc gccgcagaag ccaaagcgcg tttcgaagcg cgccaggctc gtctggagcg
    10441 cgaaaaagcg actcgccttg aacgacataa gagcgcagcc gttcaacctg cagccaaaga
    10501 taaagatgcg attgctgccg ctctggcgcg ggtgaaagag aaacaggccc aggctacaca
    10561 gcctattgtg attaaagcgg gcgaacgccc ggataacagt gcaattattg cagaacggga
    10621 agcccgtaaa gcgcaagcca gagcgaaaca ggcagaactg gagcaaacta acgacgcagc
    10681 aaccgttgct gaaccacgta aaactgccgt tgaagcagct atcgcccgct ccaaagcgcg
    10741 caagctggaa cagcaacagg ctaatgcgga accagaagaa caggtcgatc cgcgcaaagc
//
LOCUS       DAJTUI010000119        10724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTUI010000119 DAJTUI010000000
VERSION     DAJTUI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.119"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(215..290)
                     /locus_tag="OO464_003444"
     tRNA            complement(215..290)
                     /locus_tag="OO464_003444"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(255..257),aa:Ile,seq:cat)
     gene            849..3101
                     /locus_tag="OO464_003445"
     CDS             849..3101
                     /locus_tag="OO464_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228468.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3437..4369
                     /gene="csiD"
                     /locus_tag="OO464_003446"
     CDS             3437..4369
                     /gene="csiD"
                     /locus_tag="OO464_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR6228469.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4389..5657
                     /gene="lhgO"
                     /locus_tag="OO464_003447"
     CDS             4389..5657
                     /gene="lhgO"
                     /locus_tag="OO464_003447"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR6228470.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5680..7128
                     /gene="gabD"
                     /locus_tag="OO464_003448"
                     /pseudo
     CDS             5680..7128
                     /gene="gabD"
                     /locus_tag="OO464_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7136..8422
                     /gene="gabT"
                     /locus_tag="OO464_003449"
                     /pseudo
     CDS             7136..8422
                     /gene="gabT"
                     /locus_tag="OO464_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8660..10060
                     /gene="gabP"
                     /locus_tag="OO464_003450"
     CDS             8660..10060
                     /gene="gabP"
                     /locus_tag="OO464_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR6228471.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10081..>10724
                     /gene="csiR"
                     /locus_tag="OO464_003451"
     CDS             10081..>10724
                     /gene="csiR"
                     /locus_tag="OO464_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR6228472.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQ"
BASE COUNT         2758 a         2626 c         2696 g         2644 t
ORIGIN      
        1 tgcccgcttg gcatagcggg cattttttcc aggtactttt gaatgagtac tgatgaataa
       61 atacattgca gtggcgtgcc acgtaccaaa acaccagccc tcattcgaaa ccacccaccg
      121 cacttcttcc ttgaaatggc gttagtcatg aaatatagac cgccatcgag taccccttgt
      181 acccttaact cttcctgata catgaatgat gatttggtgg cccttgctgg acttgaacca
      241 gcgaccaagc gattatgagt cgcctgctct aaccactgag ctaaagggcc ttaagtatgc
      301 aataacaata cttataaacc acgcaataaa catgatgatc ttatgatgta aataacagat
      361 ttttatgcgt tcccattcac cttgggtcgt gattacacgc gacataaaac ccgacaccgc
      421 ctccattcgc aaagtcgata ctcgcagtca acaagcaaat gttaataatt agcactatct
      481 atagttatca tcgattcaat gatactttgt aatgattttg tatctaataa tataacttta
      541 ttacattagc tgaagagttt tcgcatcatt atgatatctg ttacttttca ctccataaaa
      601 ataaacttca tatagcaata tattctttca tagatcttat tctgctaatc attagtttcg
      661 tatgcgcgat ttttgacagt tgcctctcca gaccacatcg ataattaaaa aacagattta
      721 aacattatcc ttttccatat agatattgga taaaaatagg acatctgttt gcaattactt
      781 tcacaacaat taaacatttt tatgttttcg tatacatcat attactctac cattagagga
      841 acgttattat gttttctata aaaccaggac ctggaaattt acctatcgac aaccccacat
      901 tgttatcatg gaacattact gatggggatc taaattccaa atttaataca ttagaatatc
      961 taaactgtat aacaaatatt attaatgctt gtggagttta tcctcaggat ttaaaagaca
     1021 gagaaattat atcaactttt cacgcagaaa aagtcattaa tgatctgtta aaaaacgatt
     1081 ataaaatttc cctttctcca gatacaactt accgagagct gaataaagct gcacagagta
     1141 gcattacagc gccagacagg ataggagaag ggaaaacatg ggtttatcaa cgagatacaa
     1201 tggttgaaag aggtgataac agtggtgttc atcagtatgg tccagctgaa catttcactc
     1261 acattatatc tgacaaaccg tccccaaaag ataagtatgt tgcatatgct attaacattc
     1321 ctgactatga gctagcagcc gatgtatata atattaacgt gacgtcacct tccggacagc
     1381 aagaaacatt taaaatacta atcaatccag aacatctacg gcaaacactt gagcgtaaat
     1441 ctcttactgc tgttcagaaa tcacaatgtg aaatcatcac ccccaaaaaa cctggcgaag
     1501 cgattcttca tgcttttaat gccacctacc agcaaatcag ggaaaatatg tctgagtttg
     1561 cacgttccca ttatgggtat atacaaatcc ctccagtgac aactttccgc gccgacggac
     1621 cagaaactcc cgaagaagaa aaaggttact ggtttcacgc ttatcaaccc gaagatcttt
     1681 gtaccatcca taatccaatg ggggatttgc aggattttat cgcattggtt aaagatgcta
     1741 aaaaatttgg tatcgatatc attcctgatt atacctttaa ctttatggga atcgggggaa
     1801 gtggtaaaaa tgacctggat tatccctctg ctgatatacg agcgaagatc agtaaagata
     1861 tagaaagtgg tatccctggc tattggcaag gtcaggtttt gattccattc attaaagatc
     1921 cagtaacaaa agaacgtaaa caaatccatc cagaagatat acatctcact gcaaaagact
     1981 tcgaagcaag taaagataac atctctaagg atgaatggaa aaacctccat gcattaaaag
     2041 aaaagagttt aaatggaatg cctaaaacaa cccccaaaag tgaccaggtt attatgttgc
     2101 aaaatcaata cgttcgtgaa atgcgaaaat atggcgtacg aggtttacgt tatgatgcag
     2161 caaaacactc aaaacatgaa caaatagaaa gatcaataac cccaccgctt aaaaattata
     2221 atgagcgatt acacaatact aacttattta acccaaaata tcatgaaaaa accgttatga
     2281 attacatgga atatctggta acttgtcagt tgaatgaaga acaaatgtca tcgctgcttt
     2341 atgaaagaga tgatttaagc gccattgatt tttcattgct catgaagacg ataaaagcct
     2401 tttcattcgg gggagatctc ctaacccttg catcaaaacc gggttccaca atctcaagca
     2461 tcccatcaga aagacggata ttgattaaca ttaaccacga ttttcctaac aatggcaatc
     2521 ttttcaatga ctttctattt aaccatcaac aagatgaaca attagcaatg gcatatatgg
     2581 ccgctctccc gttcagcagg cctttagttt actgggatgg ccaggtatta aaatcaacga
     2641 ctgaaattaa aaattatgat gggcccacgc gtgtcggtgg tgaggcgtgg cttaataaag
     2701 gttgctctac ctatcagcag ctctacaatg aattccacgc attatatata gataaagcag
     2761 gaatatggag cgcatttgag ggtgtatttg caactaaaaa cgttctggcc tttagtcgtg
     2821 gggattctgt gaacattaat cactctcctc atgatggact agttataata aataaaggaa
     2881 acgaagaagt tgaaggtacc tggcctaaca aattgcaacc tggaatatat aaaaacatgg
     2941 ggagtaatag cgttaacatt attattaata atacccgaaa aattatcccc ccttgtaaag
     3001 tatttacgct tagaggcgga tctctaaata tcaatattcc taggcgtagc gctcttcttt
     3061 tagggaaaac aggagaaccg ccgaactatc tctatttata atttaatgtt atatctgccc
     3121 cgataaaacg gggcagataa tatgtttagt ttactaacgg tcattttgta gtgaagccat
     3181 ttactgtttt ttatcgacca gataatctgt tctctaatgt taactccccc taacctgttg
     3241 ctttagttat tcatttcctg tctcactttg ccttaatacc ctacgttaaa tgttactaat
     3301 ttgttgcttt tgatcacaat aagaaagcaa tatgtcgctt ttgtgcgcat ttttcagaaa
     3361 tgtagatatt tttagattat ggctacgaaa tgagcatcgc catgtcaccc tacatctcat
     3421 aagaggatcg cttctgatga atgcactgac cgccgtacaa aataacgctg tcgattcagg
     3481 ccaggactat agcggattca ctctcatccc gtcggcgcaa tccccgcgtc tgctggaact
     3541 caccttcacc gaacagacga ccaaacagtt tctcgaacag gttgccgaat ggcccgtgca
     3601 ggcgctggag tacaaatcgt ttctgcgttt tcgggtaggc aaaattcttg acgatctgtg
     3661 tgcgaatcag ctgcaaccat tgctgttgaa gaccctgcta aaccgtgctg aaggtgcgct
     3721 gttgatcaat gcggtaggta tcgatgatgt ggcgcaggca gatgagatgg tgaagctggc
     3781 aacggcggta gcgcatctga ttggtcgctc aaatttcgat gcgatgagcg gtcagtatta
     3841 cgcgcgtttc gtggtgaaaa atgtcgataa ctcagacagc tatctgcgtc agccgcaccg
     3901 cgtgatggaa ctgcacaacg acggcacgta tgtcgaagag atcaccgatt acgtgctgat
     3961 gatgaaaatc gatgactggg aacatctgga ccactatttc cgccatccac tggcgcgtcg
     4021 cccgatgcgc tttgccgcgc cgccgagcaa aaacgtcagc aaagatgttt tccatccagt
     4081 gttcgacgtt gatcaacagg gtcgcccggt gatgcgctat atcgaccagt tcgtccagcc
     4141 aaaagatttt gaagaaggcg tgtggttgag cgagctttcc gatgccattg aaaccagcaa
     4201 aggcattctt tctgtacccg ttcccgttgg caaattcctg ttgataaaca acctgttctg
     4261 gctgcacggt cgcgaccgct ttactccgca cccggatctg cgccgtgaac tgatgcgtca
     4321 gcgtggctat ttcgcttacg ccactcacca ctaccagacg catcagtaag cgcaaaggaa
     4381 ttgagcggat gtatgatttt gtgattattg gcggcggcat catcggcatg tcgaccgcca
     4441 tgcaactgat tgatgtttat ccggatgccc gcattgcgtt gctggaaaaa gagtccggcc
     4501 cggcctgtca ccagacgggc cacaacagcg gcgtgatcca tgccggggtc tattacacgc
     4561 ccggcagcct gaaggcgcag ttttgcctgg cggggaaccg tgccaccaaa gccttttgcg
     4621 atcaaaacgg cattcgctat gacaactgcg gcaagatgct ggtcgccacg tctcagctcg
     4681 aaatggaacg gatgcgcgcc ttatgggaac gcacagcggc gaacggtatc gagcgcgagt
     4741 ggataaacgc cgtggaactg cgcgagcgcg aaccgaatat caccgggctt ggtggcattt
     4801 ttgtgccgtc cagcggcatt gtcagctacc gcgaagtgac ggcggcaatg gcaaaaatct
     4861 tccaggccag aggcggcgag attatttata acgccgaagt cagcgccctc agtgagcata
     4921 aaaacggcgt ggtgatacgc acccgtcagg gcggtgaata tgaagcatcg acgctgatta
     4981 gctgttccgg gctgatggct gaccggctgg tgaaaatgct cggcctcgaa ccgggcttta
     5041 ttatctgccc gttccgtggc gagtatttcc gccttgcgcc ggagcataac cagattgtta
     5101 accacctgat ttaccccatt cccgaccccg cgatgccatt tttgggcgtg catctcaccc
     5161 gcatgatcga cggcagcgtg accgtcgggc caaacgcggt gctggctttc aaacgcgaag
     5221 gctatcgcaa gcgcgacttc tcatttagtg acacgctgga gattttgagc tcgtcgggga
     5281 ttcgccgggt gctgcaaaac catctacgct caggactggg cgagatgaaa aactcgctgt
     5341 gcaaaagcgg ctatctgcgg ctggtgcaaa agtattgtcc ccggctttcg ttaagcgatc
     5401 tccagccctg gcccgccggt gtgcgggcgc aggcagtatc gccggacggc aagctgattg
     5461 acgattttct gtttgtcacc accccacgca cgatccacac ctgcaatgcg ccctccccgg
     5521 cagcgacatc agcaattcct attggtgcgc atattgtcag caaggtacaa acgctgttgg
     5581 caagccagag taaccccgga cgcacgctgc gagcggcacg tagtgtggat gccttacacg
     5641 ccgcatttaa tcaataacct ttgaaaacag gacgtaacaa tgaaacttaa cgacagtaac
     5701 ttattccgcc agcaggcgtt gattaacggg gaatggctgg acgccaacaa tggtgaagtc
     5761 atcgacgtca ccaatccggc gaacggcgac aagctgggta gcgtgccgaa aatgggcgca
     5821 gatgaaaccc gcgccgctat cgacgccgcc aaccgcgccc tgcccgcctg gcgtgcgctc
     5881 accgccaaag aacgcgccac cattctgcgc aactggttca atttgatgat gcagcatcag
     5941 gacgatttag cgcgcctgat gaccctcgaa cagggtaaac cgctggctga agcgaaaggc
     6001 gaaatcagct acgccgcctc cttcattgag tggtttgctg aagaaggcaa acgtatttat
     6061 ggcgacacca ttcctggtca tcaggccgat aagcgcctga ttgttatcaa gcagccgatt
     6121 ggcgtcaccg ccgccatcac gccgtggaac tttccggcgg cgatgattac ccgtaaagcc
     6181 ggtccggcgc tggcggcagg ctgcaccatg gtgctgaagc ccgccagtca gacgccgttc
     6241 tctgcgctgg cgctggcgga actggcgatt cgcgcgggca ttccggctgg cgtgtttaac
     6301 gtggtcaccg gttcggcagg cgtggtcggt aatgaactga ccagcaaccc gctggtacgc
     6361 aaactgtcgt ttaccggtta gaccgagatt ggccgccagt taatggaaca gtgcgcgaaa
     6421 gacatcaaaa aagtgtcgct ggagctgggc ggcaacgcgc cgtttatcgt ctttgacgat
     6481 gccgatctcg ataaagccgt ggaaggcgcg ctggcctcga aattccgcaa cgccgggcaa
     6541 acctgcgtct gcgccaaccg tttatacgtg caggacggcg tgtatgaccg ctttgccgaa
     6601 aaattacagc aggcggggag caaactgcac atcggcgacg ggctggagaa aggcgtcacc
     6661 atcgggccgc tgatcgatga aaaagcggta gcaaaagtgg aagagcatat tgccgatgcg
     6721 ctggagaaag gtgcgcgtgt ggtttgcggc ggtaaagcgc acgaacgcgg cggcaacttc
     6781 ttccagccaa ccattctggt ggacgttccg gccaacgcca aagtgtcgaa agaagagacg
     6841 ttcggccccc tcgccccact gttccgtttt aaagatgaag ccgatgtgat tgcacaagcc
     6901 aatgacaccg agtttggtct tgccgcctat ttctacgccc gtgatttaag ccgcgtcttc
     6961 cgcgtcggcg aggcgctgga gtacggcatc gtcggcatca ataccggcat tatttccaat
     7021 gaagtggccc cattcggcgg catcaaagcc tcgggtctgg gtcgtgaagg ttcgaagtat
     7081 ggcatcgaag attacttaga aatcaaatat atgtgcatcg gtctttaact ggagaatgcg
     7141 aaggaacagc aataaagagt taatgcagcg ccgcagtcag gcgattcccc gtggcgttgg
     7201 gcaaattcac ccgattttcg ctgaccgcgc ggaaaactgc cgggtgtggg acgtttaagg
     7261 ccgtgagtat cttgatttcg cgggcgggat tgcggtgctc aataccgggc acctgcatcc
     7321 gaaggtggtg gccgcggtgg aagcgcagtt gaaaaaactg tcgcacacct gcttccaggt
     7381 gctggcttac gagccgtatc tggagctgtg cgagattatg aatcagaagg tgccgggcga
     7441 tttcgccaag aaaacgctgc tggttacgac cggttccgaa gcggtggaaa acgcggtaaa
     7501 aatcgcccgc gccgccacca aacgtagcgg caccatcgct tttagcggcg agtatcacgg
     7561 gcgcacgcat tacacgctgg cgctgaccgg caaggtgaat ccgtactctg cgggcatggg
     7621 gctgatgccg ggtcatgttt atcgcgcgct ttatccttgc ccgctgcacg gcataagcga
     7681 ggatgacgct atcgccagca tccaccggat cttcaaaaat gatgccgcgc cggaagatat
     7741 cgccgccatc atgattgagc cggttcaggg cgaaggcggt ttctacgccg cgtcgccagc
     7801 ctttatgcag cgtttacgcg ctctgtgtga cgagcacggg atcatgctga ttgccgatga
     7861 agtgcagagc ggcgcggggc gtaccggcac gctgtttgcg atggagcaga tgggcgtggc
     7921 gccggatctt accacctttg cgaaatcgat cgcaggcggt ttcccgctgg cgggcgtcac
     7981 cgggcgcgcg gaagtgatgg acgctgttgc accgggcggt ctgggcggca cctatgccgg
     8041 taacccgatt gcctgcgtgg ctgcgctgga agtgttgaag gtgttcgagc aggaaaatct
     8101 gctgcaaaaa gccaacgatc tggggcagaa gctaaaagac ggattgttgg cgatagccga
     8161 aaaacacccg gagatcggcg acgtacgcgg gctgggatcg atgattgcca tcgagctgtt
     8221 tgaagatggc gatcacagca aaccggacgc aaaactcacc gccgagatcg tggcacgcgc
     8281 ccgcgataag ggcctgattc ttctctcctg cggcccgtat tacaacgtgc tgcgcatcct
     8341 tgtaccactc accattgaag acgctcagat ccgtcagggt ctggagatta tcggtcagtg
     8401 ttttgatgag gcgaagcagt agcaccgtcc ccatgcagga ggcgacgctg cgcgtcttgt
     8461 ccggcctacg agaatccaag tcggataagg cgtttacgcc gcatccgaca atctgtacgt
     8521 gaacaggaat aaatctatgt cggccggata aggcggagcc gctctccggc aaaaagaatc
     8581 aataacaatt aaacgcgtga cccgacgcgg gaaatgtcgg ggcgctctcc caagtgacac
     8641 actttcgaga ggattcagga tggggcaatc atcgcaacca cacgagttag gcggcgggct
     8701 gaaatcacgc cacgtcacca tgttgtctat tgccggtgtt atcggcgcaa gtctgtttgt
     8761 cggttccagc gtcgccatcg ccgaagcgga cccggcggta ttactggcct atctgttcgc
     8821 cggattactg gtggttatga ttatgcggat gctggcggaa atggcagttg ccacgcccga
     8881 taccggttcg ttttccacct atgccgataa agccattggc cgctgggcgg gctataccat
     8941 cggctggttg tactggtggt tttgggtact ggttatccca ctggaagcca acatcgccgc
     9001 catgatcctg cactcgtggg ttccaggcat tcccatctgg ttattttccc tcgtcattac
     9061 cctcgcctta actggcagta atttattaag cgttaaaaac tacggcgaat ttgagttctg
     9121 gctggcgctg tgcaaagtca tcgctatcct ggcctttatt ttccttggtg cagtcgcaat
     9181 tagcggtttt tacccttatg ccgaagtgag cgggatctca agattgtggg atagcggcgg
     9241 ctttatgccc aacggtttcg gtgcggtatt aagcgcgatg ttgatcacca tgttctcgtt
     9301 tatgggcgca gaaattgtca ccattgccgc cgcggaatcc gacatgccgg aaaaacatat
     9361 tgtccgcgcc accaactcgg ttatctggcg tatttctatc ttctatttgt gctctatttt
     9421 tgtcgtcgtg gcattaatac cgtggaatat gccggggctg aaagccgttg gatcttatcg
     9481 ctcggttctg gaattgctca atattcccca tgcgaaatta atcatggact gtgtgatatt
     9541 actttccgta accagttgcc tgaactcggc cctgtatacc gcctcaagga tgctctactc
     9601 cttaagccgt cgcggtgatg ctcccgcggt aatgggtaaa atcaaccgca gtaaaacccc
     9661 gtacgtggcg gtgttactct ccaccggcgc agcattctta acggtggtgg tgaactatta
     9721 cgcgcctgcg aaggtattta aatttctgat cgacagctcc ggcgctatcg ccctgctggt
     9781 ttatttagtc atcgccgttt cacagttgcg gatgcgcaaa attctgcgag cagaaggaag
     9841 cgaaattcgc ttgcgaatgt ggcttgatcc ctggctcacc tggctggtca tcggctttat
     9901 tacctttgtg ttggtagtga tgctattccg tccggcgcaa cagttagaag tgatctccac
     9961 cggcttatta gcgataggga ttatctgtac cgtgccaatt atggctcgct ggaaaaagct
    10021 ggtattgtgg caaaaaacac ccgttcataa tacgcgctga taatgatcag gagtcacacc
    10081 atgaccatta cgtctctgga tggctatcgc tggctgaaga acgatattat tcgcggtaat
    10141 tttcaaccgg atgaaaaatt acggatgagt ttactgacat cgcgttatgc acttggcgtt
    10201 gggccgttgc aggaagcact ttctcaactg gtggcggaac ggctggtcac ggtggtgaat
    10261 caaaaagggt atcgggtggc gtctatgtca gagcaggagc tgctcgatat tttcgacgcc
    10321 cgcgccaata tggaagcgat gctggtcagt ctggcgattg cccgcggtgg cgatgaatgg
    10381 gaggcagacg ttctcgcaaa agcgcatctg ctcagtaagc tggaagcctg tgacgccagc
    10441 gagaaaatgc ttgatgagtg ggatctgcgt catcaggcgt ttcatacagc aattgtggcg
    10501 ggctgtggtt ctcactattt gctgcaaatg cgtgaacggt tgtttgatct ggcggcgcgt
    10561 tatcgattta tctggctgcg gcgaacggtg ctttcggtgg aaatgctgga ggataaacac
    10621 gatcagcacc agaccctgac tgcggcggta ctgccgcgag atacagcgcg cgccagtgag
    10681 ttaatgcgcc agcatttact gacgccaatt cccattatcc agca
//
LOCUS       DAJTUI010000120        10464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTUI010000120 DAJTUI010000000
VERSION     DAJTUI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.120"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            3..203
                     /gene="cspG"
                     /locus_tag="OO464_003452"
     CDS             3..203
                     /gene="cspG"
                     /locus_tag="OO464_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR6228473.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            214..402
                     /locus_tag="OO464_003453"
     CDS             214..402
                     /locus_tag="OO464_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6228474.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            377..607
                     /gene="ymcE"
                     /locus_tag="OO464_003454"
     CDS             377..607
                     /gene="ymcE"
                     /locus_tag="OO464_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCR6228475.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            597..770
                     /locus_tag="OO464_003455"
     CDS             597..770
                     /locus_tag="OO464_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR6228476.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(819..1892)
                     /locus_tag="OO464_003456"
     CDS             complement(819..1892)
                     /locus_tag="OO464_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6228477.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1994..4705)
                     /gene="torS"
                     /locus_tag="OO464_003457"
                     /pseudo
     CDS             complement(1994..4705)
                     /gene="torS"
                     /locus_tag="OO464_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4788..5816
                     /gene="torT"
                     /locus_tag="OO464_003458"
     CDS             4788..5816
                     /gene="torT"
                     /locus_tag="OO464_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR6228478.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5789..6481)
                     /gene="torR"
                     /locus_tag="OO464_003459"
     CDS             complement(5789..6481)
                     /gene="torR"
                     /locus_tag="OO464_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR6228479.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6568..7740
                     /gene="torC"
                     /locus_tag="OO464_003460"
     CDS             6568..7740
                     /gene="torC"
                     /locus_tag="OO464_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR6228480.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7740..10280
                     /gene="torA"
                     /locus_tag="OO464_003461"
                     /pseudo
     CDS             7740..10280
                     /gene="torA"
                     /locus_tag="OO464_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10277..>10464
                     /gene="torD"
                     /locus_tag="OO464_003462"
     CDS             10277..>10464
                     /gene="torD"
                     /locus_tag="OO464_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6228481.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2523 a         2685 c         2789 g         2467 t
ORIGIN      
        1 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
       61 atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat gaattccgca
      121 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
      181 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
      241 ccatgttgtc atggttcaca gtaccgtaca tcggcattcg atgtgacgga gcgaaaccct
      301 ttgggcgcta agtgtatttt ttgtaaatcg actatgatca cctttgataa cgtcgcgctg
      361 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcgtgg
      421 agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa gcgataacct
      481 gttgattatt gaatctttcg ggaagatggc ttataacatt tcttacctga ccagggtacc
      541 gggaaccaac accttactgg cgtgttgctg tctttcaaga ccagacgagg ttaacagtga
      601 atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc gcgcaaaaaa
      661 tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc atcgcacgag
      721 aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga ttttgtgaac
      781 atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat cattggcgct
      841 tgtggaatta agcgtcggta caattcctcc ggcaccgggc tttgccatac tcccgcatac
      901 attgcgtaac caatcaccgc aaacataatc cccagaacca gtagcgtcat taaccagcca
      961 gacaacgcaa aggctttttt atttgctgca ggtttttgca gtgaaaaggt caatgttgag
     1021 gctaccggac atgactctac gcaagtcata cagccggtac attccactgt tcgtacctga
     1081 attaatttat ctaccgggat ccgtgatggg cacttttttg cgcatttgcc acaatcgata
     1141 caactttcgg cattgcgacg aatcttaaac ggcgacaata gcgaaaccac gcccatcagc
     1201 gcgccatatg ggcaaagata acgacaccag gcatggcgaa taaacaggct ggcaatcagc
     1261 aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc gagcatttta
     1321 acgtccatca ccacgctata gggcgacaac ataaaatagt gaatcgcctg agcgggcatc
     1381 aataacgcga tatagagaaa taaactcaac agcaaatact tcacgccgcg cagaggaata
     1441 tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag atcgccgatt
     1501 aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt gagtaataga
     1561 ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga cggtaactga
     1621 cctgttgcaa ggctatattt cagattcatc agaccggcaa tcggtagcca gccttcgata
     1681 cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt cgtaatagcg cacccaatac
     1741 cagaacgtga tggcaatata aatattcatt gccaatagta agaattgcgt cgctttacgc
     1801 caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc
     1861 cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta agttgcatac
     1921 caaatgccac tattctagtt attcttaact ggctgatatt gattcaaatc gcgttcaggt
     1981 ctttcttatt caatcatgct tccagagcgg caacactgcg tgtaatttct tcgtgtggaa
     2041 ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctgactggcg ctacgcattc
     2101 ccagactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc tcgctgtcct
     2161 ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt ttaaataatg
     2221 ccagccattc gtggatcttc tccgtcccca ttaactgagc gtcttcattg agttgcgaga
     2281 catccagtgg tagatcgtta ttggcttgca gttggagata gtgcgccagt aactgaccga
     2341 gcacttcacg cggcaccggt ttagggataa tcccgcgaaa tagtgaactg gtacgctggc
     2401 gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac ggatattgct
     2461 gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg cagatcaaaa tccaccagtg
     2521 cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca ttgccaatag
     2581 caacaacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg gttagcgggt
     2641 tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg gctgtttttg
     2701 gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca aaaacagctt ccaacctccg
     2761 gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta ctgatagtca
     2821 gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg aagatttctg
     2881 ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc
     2941 catcagtgcg actacgcagg ataatgtgcc cttcgtcagt aaaacgcagg gcattgctca
     3001 ataggttggt aatgacctga cgaatacgtc gtggatcgcc cattaacgcg ctcggtacat
     3061 catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt
     3121 gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgact gaaacatttt
     3181 tgccacctgc ttcgatagcc gaataatcga gaatatcgtt gaggatggtc agcaacgatt
     3241 cgccagagtc ggtaatcgcc tgcaaatcat cacgctgggc gttaagtgcg gggttatctg
     3301 ccagcagttg agcagtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg
     3361 ccgtcagaaa cgccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt
     3421 gttctatcac cagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt
     3481 cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc atccatcagc cgcccgatgg
     3541 tatccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca ccgtccagca
     3601 gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gaatgagcga taaaccacgc
     3661 gccagaggat cataatcagt gcgcaaagtg aaaccatccc cagcaataac aggctgtatt
     3721 gcccgcgtgc actggctttt tccagatgtg ccagtccatg ctgattgtgc agctcaatgg
     3781 tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgata ttattttggg
     3841 cgagagtttg caggtgattg ctgatttcac tgtcctgctg gaacagcgcc aacagatcgc
     3901 tatattggct aacggtagtt aacgttgttg cgacctgcgc acgaactccc ggatcttcaa
     3961 tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg
     4021 gtgcattttt ctggatctgc tccagcccca gattcatcac catttgctgc acccgcagag
     4081 cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca atcagccgat
     4141 cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa atcccggcct
     4201 gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgctccagg cgtgcgatct
     4261 catcggcggc agctatctgc tggctgagtt gccgttgttg ctggcgtagt tgcagacgcc
     4321 gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc tgttgttcaa
     4381 tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta atcttcaggc
     4441 tttatgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg gcactggtca
     4501 ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc gccatattca
     4561 tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag cgcaggttgt
     4621 accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag cccatccaga
     4681 gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag gttgagtcgc
     4741 aaagtggaat gcatctagca taaagcctta ttattgatga ggctatcatg cgcgtactgc
     4801 tatttttact tctttccctt ttcatgttgt cggcattttc ggctgataac ctgttgcgct
     4861 ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaagcc aaacgcgtat
     4921 ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg
     4981 gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag gcaggcggct
     5041 acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg
     5101 ccattttgct cggtagtagc acgacgtcat ttcccgacct gcaaaagcag gtagcaaatc
     5161 tgccggtgat cgaactggta aatgctcttg atgatcccca ggtgaaaagc cgcgttggtg
     5221 taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg gcgcacggta
     5281 aaccactgaa cgtgctgctg atgcccggac ccgataacgc cgggggcagt aaggagatgg
     5341 tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat attgcgcttg
     5401 gtgataacga tattgaaatc cagcgcaacc tgttgcagga gatgctggaa cgccacccag
     5461 aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt gaagggcgta
     5521 acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc
     5581 tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg
     5641 cagttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat gtcagcccac
     5701 cgattttagt tctgacgccg aaaaatgccg accgagaaca tatccgccgc tcgctgtcac
     5761 cggggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag aaataacctt
     5821 caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga cgaattaaca
     5881 catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata cgcagcagac
     5941 gctcacggct gagaatttcg cccggattag tcacaaatgc caccaacatt tcatactctg
     6001 cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac acattcaggc
     6061 aataacccgc aaagcgatag cagtagtcct gagtgtgcgg ttgagcttgt cgcgcgaggt
     6121 cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc agcggtttgg
     6181 tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc
     6241 cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc
     6301 cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga ttctgcataa
     6361 tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc tgagtgaagt
     6421 aggattgtaa tcgcgcctgg gtaaccggct tatcttcaac aataacaatg tgatgtggca
     6481 tcagagggtt ttactcattc tgttcatatc tgttcatatt tgctcattaa gatcgcttca
     6541 ctaaaccata attctacagg ggttattatg cggaaactct ggaacgcgct acgccgaccc
     6601 agtgctcgtt ggtcggtact ggcgctggtc gcaattggga ttgtgattgg cattgcgctg
     6661 attgtattgc cacacgttgg gatcaaagtc accagcacaa ccgaattttg tgtcagttgc
     6721 cacagtatgc aaccggtgta tgaagaatat aaacagtcgg tgcatttcca gaacgcctcc
     6781 ggcgtgcgag ctgaatgcca tgactgtcat atcccgccgg atattccagg catggtgaag
     6841 cgcaaactgg aagcgagcaa cgacatttac cagaccttta ttgcccactc cattgataca
     6901 cctgaaaaat tcgaagccaa acgcgcggaa cttgccgagc gtgaatgggc gcgaatgaaa
     6961 gaaaacaact cggcaacctg tcgctcctgc cataactacg atgcgatgga tcatgccagg
     7021 cagcatcctg aagcggcacg tcagatgaag gtggcagcga aagataatca atcctgcatc
     7081 gactgtcata aaggtattgc ccaccagtta ccggatatga gtagcggctt ccgtaagcag
     7141 ttcgatgagc tgcgcgccag tgctaatgac agtggtgaca cgctgtactc tattgatatt
     7201 aagccaattt atgcggcgaa aggcgataaa gaagcctctg gttctctgct gcctgcttcg
     7261 gaagtgaaag tccttaaacg tgacggcgac tggctgcaaa ttgaaattac cggctggacg
     7321 gaaagcgccg gacgtcagcg tgtactcacc caattcccag gtaaacgcat ctttgttgcc
     7381 tcgattcgtg gtgatgtgca gcagcaggtg aaaacgctgg agaaaaccac cgttgccgac
     7441 accaataccg agtggagcaa gttgcaggcc accgcgtgga tgaagaaagg cgacatggtg
     7501 aacgatatca aaccgatctg ggcctatgcg gattcgttgt acaacggcac ctgtaacgag
     7561 tgccacggcg caccggaaat cgcccacttt gacgctaacg gttggatcgg cacgctcaac
     7621 ggcatgattg gctttaccag tctcgataaa cgtgaagaac gcactttgtt gaaatatctg
     7681 caaatgaatg cgtctgacac cgcaggtaag gctcacggcg ataagaagga agaaaaataa
     7741 tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct
     7801 taaccgtcgc cgggatgctg gggccgtcat tgttaacgcc gcgccgtgca actgcggcgc
     7861 aagcggcgac tgaggctgtc atctcgaaag agggcattct taccgggtcg cactgggggg
     7921 ctatccgcgc gatggtgaag gatggtcgct ttgtggcggc aaaaccgttc gaactggata
     7981 aatatccgtc gaaaatgatc gccggattgc cggatcacgt acacaacgcg gcacgtattc
     8041 gttatccgat ggtacgcgtg gactggctgc gtaagcgcca tctgagcgac acctcccagc
     8101 gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc cctcgacatg ttctatgaag
     8161 aactggaacg cgtacagaaa actcacgggc cgagtgcctt gctgaccgcc agtggttggc
     8221 aatcgacggg gatgttccat aacgcttcgg ggatgctggc gaaagctatt gccttgcatg
     8281 gtaatagcgt tggtacgggc ggagattact ctaccggtgc tgcgcaggtg atcctgccgc
     8341 gcgtagtcgg ttcaatggaa gtgtatgaac agcaaacctc ctggccgctg gtattgcaga
     8401 acagcaaaac cattgtgctg tggggctccg atttgctgaa aaaccagcaa gcgaactggt
     8461 ggtgcccgga tcacgatgtt tatgaatatt acgagcagct aaaagcgaaa gtcgccgccg
     8521 gtgaagttga ggtcatcagc atcgatccgg ttgtcacatc cacccatgag tatctggggc
     8581 gcgagcatgt gaagcacatt gcggttaacc cgcaaactga cgtgccgctg caactggcgc
     8641 tggcacatac gctgtacagt gaaaacctgt acgacaaaaa cttcctcgct aactactgtg
     8701 tgggttttga gcagttcctg ccgtatctgc tgggtgagaa agacggtcag ccgaaagatg
     8761 ccgcatgggc tgaaaaactg accggcattg atgccgaaac cattcgtggg ctggcgcggc
     8821 agatggcggc gaacagaacg caaattattg ctggctggtg cgtgcagcgt atgcagcacg
     8881 gtgaacagtg ggcgtggatg attgtggttc tggcggcgat gctggggcaa attggcctgc
     8941 caggtggtgg ttttggctgg cactacaacg gcgcaggcac gccggggcgt aaaggcgtta
     9001 ttctgagtgg tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgactaca
     9061 aaggctacag cagcactatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga
     9121 aagtgatcaa ctggaacggt aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg
     9181 ccggaactaa cccattccat cgccatcagc agatcaaccg cattattgaa ggctggcgca
     9241 agctggaaac ggttatcgcc atagataacc agtggacctc aacctgccgc tttgccgata
     9301 tcgtgctgcc tgcgaccacg cagtttgagc gtaacgatct cgaccagtac ggcaaccact
     9361 ccaaccgtgg cattatcgcc atgaaacagg tggtgccgcc gtagttcgag gcgcgcaacg
     9421 actttgatat tttccgcgag ctgtgccgtc gctttaatcg tgaagaagcc tttaccgaag
     9481 ggctggacga aatgggctgg ctgaaacgca tctggcagga aggtgtacaa caaggcaaag
     9541 gacgcggcgt tcatctgcca gcgtttgatg acttctggaa taacaaagag tacgtcgagt
     9601 ttgaccatcc gcagatgttt gttcgccacc aggcattccg cgaagatccg gatctcgaac
     9661 cgctgggcac gccgagtggc ctgattgaga tctactcgaa aaccatcgcc gatatgaact
     9721 acgacgattg tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc
     9781 ccggctcgca gaagtatccg ttgcatctgc aatctgtgca tccggatttc cgacttcact
     9841 cgcagctatg tgagtcggaa acgctgcgtc agcaatatac ggtagcgggt aaagagccag
     9901 tgttcattaa cccgcaggat gccagcgcgc gcggtattcg taacggtgat gtggtacgcg
     9961 tctttaacgc tcgcggtcag gtgttggcag gagcagtagt ttctgaccgc tatgcacccg
    10021 gcgtggcgcg aattcacgaa ggggcatggt acgatccaga taaaggcggc gagcctggtg
    10081 cgctgtgcaa atacggtaac cccaacgtgc tgaccatcga catcgggact tcgcagctgg
    10141 cgcaggcgac cagtgcgcac actacgctgg tggaaattga gaagtacaat ggaacagtgg
    10201 agcaggtgac ggcgtttaac ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg
    10261 cgtcgcaggt gaaatcatga ccacgctgac agcacaacag attgcctgtg tttacgcctg
    10321 gctggcgcag ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc
    10381 gcagatggct gaatggtttt cgttgctgaa aagcgaaccg ccgctcactg cggcggtgaa
    10441 tgatctggaa aacagtattg ccac
//
LOCUS       DAJTUI010000121        10435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTUI010000121 DAJTUI010000000
VERSION     DAJTUI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.121"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..272)
                     /locus_tag="OO464_003463"
     CDS             complement(<1..272)
                     /locus_tag="OO464_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6228482.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="OO464_003464"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="OO464_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1132..1338)
                     /locus_tag="OO464_003465"
     CDS             complement(1132..1338)
                     /locus_tag="OO464_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR6228483.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1445..2008)
                     /locus_tag="OO464_003466"
     CDS             complement(1445..2008)
                     /locus_tag="OO464_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6228484.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3383..5218)
                     /locus_tag="OO464_003467"
     CDS             complement(3383..5218)
                     /locus_tag="OO464_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228485.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5319..5606
                     /locus_tag="OO464_003468"
     CDS             5319..5606
                     /locus_tag="OO464_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6228486.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5578..7107
                     /locus_tag="OO464_003469"
     CDS             5578..7107
                     /locus_tag="OO464_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR6228487.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7277..8494)
                     /locus_tag="OO464_003470"
     CDS             complement(7277..8494)
                     /locus_tag="OO464_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6228488.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8861..9847)
                     /locus_tag="OO464_003471"
     CDS             complement(8861..9847)
                     /locus_tag="OO464_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228489.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9844..10372)
                     /locus_tag="OO464_003472"
                     /pseudo
     CDS             complement(9844..10372)
                     /locus_tag="OO464_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3249 a         2386 c         1965 g         2835 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg gggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagagaga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acagacacac aataccataa ctccaactaa taatactaat taaccttaat
     2761 tatttatggt atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga
     2821 tgatcactgt ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg
     2881 agtgaccagt aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata
     2941 agccactaac ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat
     3001 catgatttgg cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca
     3061 agagcttaca ccgaattaca gattatgtat taatgtcaaa actggcatat tttacagata
     3121 caatgaacat gcaacccatc aatctaatag gggcggtatg tacatctgcc catgaatggg
     3181 atggcgctaa gaatacaaaa caattagaca tagatcacaa aaacacacca ttctaattga
     3241 aaaacaaaac aaacaaataa atcatactat tatagaattt tctcttgcca gacatagata
     3301 atacccctgc taattttgct aatggttagc aaaattagca gggtatataa agattattga
     3361 cccaaaacac actcagatga attcacgaca gctcaggagg gttattcctg atgtcatctt
     3421 cacaaataat ggttaacgca tcatatatca ttctgcgtct gttcctgttt tcggccttat
     3481 cgccatacaa catcatcaca tttttaattt catcgaccgg tgatttcaca tagtgaacta
     3541 atacaccaag cttctcaaca cattgcagta aagtatattt cttactgcct gtgaaaagcc
     3601 ctatcatagt ttctttacgt tcgcgactgg ataatacttt gttttccata atgacttctg
     3661 ccagcgtctt accatcataa acatactctt tatctatcag aatatttatc ttatccagca
     3721 ccttctctac gtcatcaagc aaacctgtaa tatatggggg cgcaatatat ggtttcgtac
     3781 tgatcttttc aagcatacaa ttatacatct taataacacg catcagggta aaattgttcg
     3841 catcatactg tatttcaccc tccttatata caggatcaat aagtggtatt ttttcaataa
     3901 atgcatcctg ataggctttt acatggtcaa gccaataaaa caacgagcca tactttatat
     3961 tttttctatt tactttaacc tccagtgctg tccgaagcaa cgataatgcc cccctgacat
     4021 atcctgaagg taattcacca aacaatcctt ttctttccat gtcatcataa tgctgcagca
     4081 catcattaat tttatttttc cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc
     4141 tggtcacatg aacagcctca ttctgcaact gcccaaaata gcttataatc tttttcagat
     4201 atgcgtcatc acaaccattc ttatcattaa tattaaatac aagatctttc agacgggaaa
     4261 tatattcctg agatttatca tcaagtgaaa cactataacg agacaatgtt ccttctgcac
     4321 tgttttgaaa cttataaaac atttccagta atttttttgc ataaggcgtt gtatcaaagc
     4381 tggtaaagtc ataaagatat tgcgcagcgt aaccatacaa accatgaatc cagagtcctt
     4441 ctctcaccaa cactctgaga taatatgcaa ggctttgaag tatcttcgtc tttcgttgta
     4501 tatcaagtaa gacacttaat gaataaaatg cattatagaa tttatcaata ttcctgagca
     4561 ctttctccag ttcatcctta tccagcttct tctcccggta atccttttta tattcatttt
     4621 tgatagccat gatatattgt tgcatcaggc tcttgctttt cccgttatcc ttccgtatag
     4681 ttcctgtata tttccccaga gcatacagct ttccattttc atgaaaatct tccttgttga
     4741 atcccagttc agccagatat tcccgaagat aacgtttaac agcagcttca tcgctttctg
     4801 tatcagcgtg atcaagacaa ggacaatacg atttctgtaa aaaattataa atatgataat
     4861 aaaaagcaga gtcattctct ggtctgtatg tattatacat atctacaaca agaaaaataa
     4921 ccccaaaagt gcgcagacaa tagctgcgaa ggaattcagg ttttagtgtg gaatgcactg
     4981 gataagaatt catcatgtaa taaaaatgtt catatcgggc atatatgatc cgccgttgct
     5041 cctgactgaa caccccttca gcatcaataa tacgactgat ctcattataa cgttgatccc
     5101 agaatttttg atgcatttca aaattggtac tcttccgctt gtgtgccgga ttctcgcctt
     5161 ccttaaacaa ccccaaaaca gtacaaatat catacaacat atttgtaaat gtgcgcatat
     5221 gctgtgctct ccggttggtt tgcattttat cgcagaggac attctgagct taacgtatca
     5281 aactgacaca acgacatgca atgcatatgg agaacaatat gtcaaatcaa accggacagc
     5341 tttacacgcc ttcacgcatc ctgcgtatgg ctgaacttac ccgactgctg ggtatatcaa
     5401 gatctactat ttacgtgaaa ataaatccgg cctcgaaata ccacgaccct acctttccca
     5461 aaccaataag gcttggtagt gcatccgtag gctggcgggc atctgccatc gatgagtggc
     5521 tgatgttaca tacggcacca gcctggtctg aacctgaaaa caaactaaag gaacttaatg
     5581 atgacgacaa aactatcact ccctgaactt gacacaacta aactgcctga caggtgccag
     5641 gcattacttg atgaaatgca tgaagaaaca ggaatacgcc gtgaaatact tctgtctgtt
     5701 atgctgacgg tcatggctgc ctcggtccag gatacgcatg aagttgaact ttccggaggg
     5761 caacgtacca gccttcagat atttatgtgt ctttcatcag catcaggcag tggaaaaacc
     5821 tctgcctgcg caaaattaat cgctcctgtt catgaaacag aagaagaact acatcaggca
     5881 tatatcgacg ataaaaaaaa ctatgaccga atgatggaaa tgtggacaac agataaaaaa
     5941 atcctggagc ggagatataa aaaggaaatt gaaagatccc cggaaaatgc tgctgctgca
     6001 cgagcagcac ttgaaaagtg tatagcaaat aaacccgaac ctcccgtaca acaggttctc
     6061 atcgtgaatg acgcgacacc agaagggata gctttgaaac tcagccagtc accctctctt
     6121 ctgttgctgt ctgatgaagg aggtactatt ctggataagc gtttcgaacg caaatcggca
     6181 ctgtacaaca cattatggag tgggcaacca gtcaacgttg aacgggcatc cagacccggg
     6241 ttccggctga atgatgtccg tctcactata cttatactga ctcaaccggt aatatttaat
     6301 aagtttttta ctctcactgg cgatcagatc cggggcaatg gctttctggc ccggttgcta
     6361 ttttgcgaac ccggagcaat taaaataatg acgacagaac cgcatactgc cactcctgtt
     6421 gtgacgcaac aatgtgcaag ctgttttcgt aagaaaagtt ttggcagtca gatcagagac
     6481 tctctgagag catccagaga aagacgtgca aaaagtaaag agcgcatctg catgacatta
     6541 tcaaatgcag catcccgcgc actggagcgc tttcacaaag aaaatatgga tactgtcaga
     6601 cagaatcctc atatgggtac atttgaagac attattgtca gaaaaagaga gcaggttgtc
     6661 aggatagcag cacttctgga actggagaaa gatccagact cgacagtaat aacgctggaa
     6721 agtactaata gcgccattta tctcattgaa ttttatttca agcatcttat atataaactt
     6781 gaatcccttc gggaaatatc cccggctgag aaacttgata aatggcttca gaaaagaatt
     6841 atcacaacag caggttacat attccagaaa agttatattt tacaatatgc cccatatgct
     6901 ctcaggaaaa aatgtgttct ggatgaagca cttgacatac tggcggaaca aaggaaaata
     6961 aggattgatg ataacctggt tgtttatata ggaaacacga ttacaccatc cgaactggca
     7021 aaaaaactaa atattcctgc attcgatgcc ggagttttca tttgtgatca tcaaaacata
     7081 ctgaagtatc accgaaacag actatagata ctatcggaca aaaaataaat tatcacaagt
     7141 gtattattcg ttataataat gaataatcgt tatctgagtg caatgccagg ttttcattac
     7201 gaactggcat tgcatttatt tttcaaaaaa gaaagcagta accatttggc tactctgagt
     7261 atgccagtcc atgaaactaa gcgaattcct tagtgttttt agcaagttga gacatcacat
     7321 aatccgccca tgcctgcatc atcggtacac gctgctccag tagatcagta cgatgatatg
     7381 ccgcctcaac cttatttttc agcgtatgag cgagcgccct ttccgccaga tcccgcgaat
     7441 acccctgttc actacaccag tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc
     7501 gccccggaat gtcactgacg gctttctttt tacgcagaaa acttgtcaac accatatcgg
     7561 aaaggatctg ctgctttctg ggtgaaggga acaccagttc atcatgcagg ccacgtatat
     7621 tttccagaat gtaaatagcc tgccgggata aaggaacacg atgctgtagc ctagctttca
     7681 ttctttctgc aggtatagtc catacccgct tatgaaaatc aatttcagcc cagcgcattc
     7741 ccctggcttc gcccgagcga gttgctgtaa gtatcaccat taataacagt gcgcgggtaa
     7801 cattataagg ttcatcacta tacacactgg tcgccacaaa aagcggtaac tgcctccagg
     7861 gcattgcggg ttggtgttca tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca
     7921 catcaacagg atttgccaca caaaaaccgt gcgcccatcc ccactgcata acaacatgaa
     7981 tgcgctgttt aacccggctt gccgtttctg acaaggttaa ccagactgga cgcagtgttt
     8041 ctgccacgtc cgcagccgta atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt
     8101 aattctcaag cgtcgataac cactgcctta catgctttgg attctcccat ccaggagaca
     8161 gttctgcatg tacacgcctg gctgcatcgg caaatgttgg gataacgact ttctcagatt
     8221 cagccttttt aatctccaga ggatcatcac ctgcagcaag ttgctctcgc attatccggg
     8281 cagtacgtgc agcttcagca atactgacct ctgggtaagt tcccaatcca gcattacgtc
     8341 ttttttgtgt caccggactt acataacgaa aaacccattt cccccgcccc tttactgaag
     8401 aaggatgaag ggtcagtccg gtaattcccc catggggcaa tggtttgtca tcaggtttga
     8461 tatgtcttgc tttcgtatcc gtcaatactg ccatacgcta attcccgtca ctctggtatg
     8521 ccatccagta tgccattagt ccctcgcttc actaagaata cattagataa catcggacaa
     8581 caaaaagtgt aatacattgt ttataaagaa attatataac acccctggat taagtcagat
     8641 ttatttcagg cggtccccct caccgccata tttaaagaag agcgcgtacg aaagtacggg
     8701 cttttttttc gtatattgta cacaccgggt gcccccgccc acgtctgtta gggcgagggg
     8761 aaattgtgca catcaactgt ctggttactc agtcaacaat caaaatgcgt aagatagtac
     8821 agaacatata tgcgttatct ggtcacttcc tgtaacttcc tcacttatta aaatgccccc
     8881 aacggaaata catgtcacat gccatcataa aatcgttatg ccagaggaat cgacaatgga
     8941 aaaaatagta aattaagcac ggtacatagc agagtaaaaa ataggcttca aaccaaatcc
     9001 gccattccac aagcgttgcc aataccgaca ccagcgtaat ccacatcatt tcaataaccc
     9061 gcaatatgaa taaccgaagc caaagcggct tggcaaaaaa ggtcgacaac gtattttcac
     9121 tggagaaaaa cagatcgatt tcctgcaaaa aaaacctgcc cagaaaagcg ggccgcttgg
     9181 agaagcgcag aaaaaacaga ttaatcgcca acactacgcc acaaagaatg agcaatgcaa
     9241 ttatcgattt aaacgttaac cactgcccct ctgtcacaaa agaatcactg ttacgcagcg
     9301 ggctgatagt aagcaggtta acaaccgttg agatgcacac cgaaagcagc acatagcgag
     9361 tcagactttt atattttact tttttcgcta ctgaaatagc gacctgcttg acataactgg
     9421 tgcggatcat ccgaaacagc agaaagatga ccaacggccc aagtaacgaa atcaggaaca
     9481 gcgcatataa cgagggtaga agctggtata gcccccaact cattgcactc aacatgatta
     9541 acgacagaaa tcctgccacc agaggttgtg taagaaataa ttctattttg tggttctgct
     9601 ttttcttaaa ccacgaaaaa tcgatattct tcgtgacgca tttataagcc cagtacgcct
     9661 tgagatcaaa aataaaatta tacagcacca gggccagaat cccactaact acaccaatat
     9721 cctctgatgg aaaagtaaac cctgcatttt tccaataaaa gcctatcaga ataagtccaa
     9781 tagcataaat gtatatcaga tttcgtttat cctgaaagcg aataaaagtt aaataatcag
     9841 gaatcatttg aatacctttg catcactaaa tgctttacga caatgcataa ctcgttgctg
     9901 atccacgcca gcaaaatcat ttaacatatt ccccaaatag ggcttattac ttatttcaag
     9961 gaaagctacc ggggttggca tattcagaat atgtatcgag agattcagtc catttcaccg
    10021 tatgagttaa gtgtaatgag agattttctt tgataattga cggagcgacc tcgctttttg
    10081 ctgttacgtt catcagaacc tgatgctctg gcgaagcgat atccagtccg gcaagataat
    10141 cgcgcattgc ctgaacgccg tcttccatca aacgcgtgtg ccaggcaccg ctcacaccga
    10201 gtttaaccgg ttcgtatcca gcggccatca gcagcgtggt aaattcattc aacgcagcct
    10261 gcgttccccc aataacctgc tggcgcggcg tgttatcaca gctaatatcc agcgcaatgc
    10321 ctgattccgt aatcatcgtc tgcagttgtt cgcggttgat gcctttaacc gcctgatctt
    10381 acccagcaat agtggacacg cggctaagtg agtaaactct cagtcagagg tgact
//
LOCUS       DAJTUI010000122        10207 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTUI010000122 DAJTUI010000000
VERSION     DAJTUI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10207
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.122"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            405..536
                     /locus_tag="OO464_003473"
     CDS             405..536
                     /locus_tag="OO464_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR6228490.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            796..1791
                     /gene="pgl"
                     /locus_tag="OO464_003474"
     CDS             796..1791
                     /gene="pgl"
                     /locus_tag="OO464_003474"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6228491.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1832..2785)
                     /locus_tag="OO464_003475"
                     /pseudo
     CDS             complement(1832..2785)
                     /locus_tag="OO464_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2969..4021
                     /locus_tag="OO464_003476"
     CDS             2969..4021
                     /locus_tag="OO464_003476"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR6228492.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            4097..5530
                     /locus_tag="OO464_003477"
     CDS             4097..5530
                     /locus_tag="OO464_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR6228493.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDEVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5713..7893
                     /locus_tag="OO464_003478"
     CDS             5713..7893
                     /locus_tag="OO464_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6228494.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(8034..9317)
                     /locus_tag="OO464_003479"
     CDS             complement(8034..9317)
                     /locus_tag="OO464_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6228495.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9452..10144)
                     /locus_tag="OO464_003480"
     CDS             complement(9452..10144)
                     /locus_tag="OO464_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6228496.1"
                     /translation="MLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPVAATRA
                     AKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGY
                     LYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVS
                     RYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRD
                     DRGREWDKIEIK"
BASE COUNT         2502 a         2372 c         2554 g         2779 t
ORIGIN      
        1 aatgagtgtt atatcatgct ttctaccctg gtaagtatat ttgctttttt cgtatatttg
       61 gttcatcttt ttttgatgtt ttacatattc acaatgcaga ggatttatac cagaagacat
      121 tagtcttgag taagatacta caacatctcc aactatttta gccagcctga ctacttgact
      181 tggtaatatt tgttgctaat aatcatgtat aaaataagct tatatacata gtaacttcag
      241 caaaggattt agtaatgatc aaaatcatat ctcaatgtac tgtttttttc tttaaaaaaa
      301 cagtacattc tattttataa tgactgtgta ttcccatgga aggagatcac ccaattctcc
      361 cccccgactt ttgacgcagt gtcttgttaa cagggggatt gaccatgctt attggctatg
      421 tacgcgtatc aacaaatgac cagaataccg atctacaacg taatgcgttt atttgtgccg
      481 gaagtgagtt gattgttgcg cgaatgggaa gttggccaga agttgttatg gcatagtgtt
      541 tacggtcgtt gatacaggta aacaaaagcg gggagagtca tactccctga atagtcgcga
      601 aaatatctgt ggcattgtca aattcgtgtc ggtgactaat attttagtaa tatgccattc
      661 gaaatggagt gctaacttat tgatttaatt gatgtgcgat attgtatttt atcgtgtttt
      721 gttgtctgtt ttttatccag taagtgttgt tatatgtatg aaagataagg gatttatcat
      781 ttttgaggtg tgttcatgaa gcaaacagtt tatatcgcca gccctgagag ccagcaaatt
      841 cacgtctgga acctgaatca tgaaggcgca ctgacgctga cacaggttgt cgatgtgccg
      901 gggcaggtgc agccgatggt ggtcagcccg gacaaacgtt atctctatgt tggtgttcgc
      961 cctgagtttc gcgtcctggc gtatcgtatt gccccggacg atggcgcact gacctttgcc
     1021 gcagagtctg tgctgccggg tagtccgacg catatttcca ccgatcacca ggggcagttt
     1081 gtctttgtag gttcttacaa tgcgggtaac gtgagcgtaa cgcgtctgga agatggcctg
     1141 ccagtgggcg tcgtcgatgt agtcgagggg ctggacggtt gccattccgc caatatctca
     1201 ccggacaacc gtacgctgtg ggttccggca ttaaagcagg atcgcatttg cctgtttacg
     1261 gtcagcgatg atggtcatct ggtggcgcag gaccctgcgg aagtgactac cgttgaaggg
     1321 gccggcccgc gtcatatggt attccatcca aacgaacaat atgcgtattg cgtcaatgag
     1381 ttaaacagct cagtggatgt ctgggaactg aaagatccgc acggcaatat cgaatgtgtc
     1441 cagacgctgg atatgatgcc ggaaaacttc tccgacaccc gttgggcggc tgatattcat
     1501 atcaccccgg atggtcgcca tttatacgcc tgcgaccgta ccgccagtct gattaccgtt
     1561 ttcagcgttt cggaagatgg cagcgtgttg agtaaagaag gcttccagcc aacggaaacc
     1621 cagccgcgcg gcttcaatgt tgattacagc ggcaagtatc tgattgccgc tgggcaaaaa
     1681 tctcaccaca tctcggtata cgaaattgtt ggcgagcagg ggctactgca tgaaaaaggc
     1741 cgctatgcgg tcgggcaggg accaatgtgg gtggtggtta acgcacacta accgctgatt
     1801 taaccggcgc agtctctcct gcgccggtgt attaacttat ctcctgtaac gcatgtctct
     1861 ggcgttcgac gatattggtc cacaaattgt ctttatcgtc agtccacaaa ttaatcagca
     1921 aggaaaaaaa gcgttcagct gccggagaaa gcacggcatc tttgcggcga ataatcccca
     1981 atgtccgacg tataacgggt tccaccagtg ggataccaat aatcgaagaa tagggcgcat
     2041 ggggcattgc cagaccagga agcgccgaaa tccccagtcc tgcctccact agtcctaatg
     2101 acgtcgaaag atgacgcact tcgtaaaacc agtccagctt ccagggcttg tcggctagct
     2161 gttgttctat caacaatcgg ttgccgctgg aggaacgtac gccaatcatt ttgtaaccca
     2221 ctaactcctg ccattctaca agctactttt tggccaatgg atggtcacgc cgacaggcca
     2281 gcacgaatgg ctcgttgacc agtggggtaa aatcaatgga tgaatttgtg acgttgttca
     2341 tgtttatgcc aaagtctgat tcgttacaca gcaccgattc catgcaatta ttcgtgcctt
     2401 gttccagaat ccgcacttta atattgggat acagctcatt aaatttaccg attgccagcg
     2461 gtaaaaaata aaatactgcg gtcggaatac acgctaatgt caccatacca cgatgataag
     2521 cattcatatc acgaatatta aaaagcgttt catcgaattt ctttattaat tcccgcgcct
     2581 ccgggagcaa cctttttcct gcttttgtta acgtaacctt gcgagttgta cgctcaaata
     2641 gttgaatatg taaatcttcc tccatctttt ttattcttcg cgttaatgca ggttgcgtaa
     2701 tattgagtaa tttagcggca ttgttaaatg aactggactc tgccagtatg acaaatgcct
     2761 tcatacttga taattcatgc ttcattatta ctccggaaaa tggaagcgac gattttgggg
     2821 ggctggtcgt taaaaatttt aactgcattt agccaactta aattaatgaa aaaatgttat
     2881 taatcgttga gctaaagtca ttagagatgc tttgtcctta atgtaaccat atcgcaataa
     2941 gttatgtttt taaattgagg gcattattat gaaaaaaata ccctgcgtga tgatgcgagg
     3001 tggaacctcg aggggcgcgt tcctgttagc ggaacattta cccgaagatc aaacgcagcg
     3061 cgataaaata ttgatggcaa ttatgggttc cggtaacgat ctggaaattg acggtattgg
     3121 cggcggtaat ccactgacca gtaaagtcgc cattattagc cgttccagcg atccgcgtgc
     3181 tgatgtcgat tatctgtttg ctcaagttat cgtccatgag caacgtgtcg ataccacgcc
     3241 taactgcggc aatatgctgt ctggcgtagg ggcattcgcc attgaaaatg gtttgattgc
     3301 agcgacttcg ccagttaccc gtgtacgtat ccgcaacgtc aatacgggta cgttcatcga
     3361 agctgatgtg caaacgccaa atggtgttgt cgagtacgag ggtagcgcca gaattgacgg
     3421 cgtaccgggt actgccgcac cggttgcgct cactttcctg aatgccgctg gaacgaaaac
     3481 tggaaaagtt ttcccgactg ataatcagat tgattatttt gacgatgtcc cggtgacctg
     3541 tatcgatatg gcgatgccag tcgtcattat tccggctgaa tatctgggaa aaacaggtta
     3601 tgaattaccg gcggaactgg atgccgacaa agcattatta gcccgcattg aatctatccg
     3661 gctacaagcg ggtaaagcaa tgggcttagg tgatgtcagt aatatggtta tccctaaacc
     3721 tgtgcttatt tctccagcgc agaaaggcgg ggcaattaat gtgcgttatt ttatgccgca
     3781 ttcttgccat cgcgcgctgg cgataaccgg tgctattgct atttccagta gttgtgcatt
     3841 ggaaggcacc gtcacccgac aaatcgtccc ttctgtagga tatggcaata tcaatattga
     3901 acaccccagt ggtgcgctcg acgttcattt aagtaatgaa ggtcaggatg ccacgacgtt
     3961 acgcgcatct gttattcgga caaccagaaa aatattttcc ggtgaagttt atcttccctg
     4021 aaaaaattcg ttgtcaggat aaggacaatc aataaaggac ttctgtatga gtcatacaga
     4081 aagaacagga ttttaaatga ataagaaatc gttatggaag ctaattctga tattagcgat
     4141 cccatgtatt attggtttta tgccagctcc ggcaggatta agcgaactgg cgtgggtgct
     4201 ttttggtatt tacctggcgg ccattgtggg tctggttatc aagcctttcc cggaacctgt
     4261 cgtactgtta attgccgttg ctgcctcaat ggtggtggtc ggtaacttat ccgacggtgc
     4321 gtttaagacc accgccgtat taagcggtta ctcttcaggt actacctggc tggtgttctc
     4381 ggcgtttacc ttaagcgccg catttgtgac caccggttta ggtaaactta ttgcctatct
     4441 gctgattggt aaaatcggta acaccatgct gggtctgggt tacgttacgg tattcctcga
     4501 tctggtactg gctccggcaa caccgtctaa caccgcgcgt gcgggcggca ttgtgttacc
     4561 gatcatcaac agcgtggcgg tggctttggg gtccgaaccg gaaaaaagtc cgcgtcgtgt
     4621 cggacattac ctgatgatgt ccatttacat ggtcaccaaa accaccagct atatgttctt
     4681 taccgcaatg gcggggaaca ttctggcgct gaaaatgatc aacgacattc tgcacctgca
     4741 aattagctgg ggtggatggg cgctggccgc cggattgccg ggcatcatta tgctgctggt
     4801 caccccgctg gtaatttaca ccatgtatcc accagaaatt aaaaaggtgg ataacaaaac
     4861 catcgctaaa gcgggccttg ccgaactggg accgatgaaa atccgcgaaa aaatgctgct
     4921 cggtgtcttt gtgctggcgc tactgggctg gattttcagt aagtctctgg gggttgatga
     4981 atccactgtg gcaatcgttg ttatggcgac catgctgctg ctgggtatcg ttacctggga
     5041 agacgaggtt aaaaataaag gcggctggaa taccttaatc tggtacggcg gtattatcgg
     5101 cttaagctcc ttattatcga aagttaaatt tttcgaatgg ttagctgaag tctttaaaaa
     5161 taacctggca tttgatggtc acggtaacgt tgctttcttc gttattattt tcctcagcat
     5221 catcgtgcgt tatttcttcg cttccggtag tgcctatatc gttgctatgt tacctgtatt
     5281 tgccatgctg gcgaacgtct ccggcgcacc gttaatgtta accgcgctgg cactgttgtt
     5341 ctccaactcc tatggcggca tggttactca ctatggcggc gcggcaggtc cggtcatctt
     5401 tggcgtgggt tacaacgata ttaaatcctg gtggttggtc ggtgcggtac tgacgatatt
     5461 aaccttcttg gtgcatatca ccctcggcgt gtggtggtgg aatatgctga tcggctggaa
     5521 catgctgtaa atatactcgt catacttcaa gttgcatgtg ctgcgtctgc gttcgctcac
     5581 cccagtcact tacttatgta agctcctggg gattcactca cttgtcgcct tcctgcaact
     5641 cgaattattt agagtatatc catttattat ctttctgcgc acttcacggt gcgcagatat
     5701 ctggagcatt tgatgatcaa gttatctgaa aaaggcgtgt ttctcgccag taataacgaa
     5761 ataattgccg aagaacattt caccggcgaa attaaaaaag aagaagcaaa aaaaggcact
     5821 attgcctggt ctattctctc ttctcataat acgtccggaa atatggataa acttaaaatt
     5881 aagtttgatt cattagcctc tcacgatatt acctttgttg gtattgtaca gaccgctaaa
     5941 gcgtccggaa tggaacgttt cccgctgccg tatgtgctga ccaactgcca taactcactc
     6001 tgcgccgtcg gcggcaccat taacggtgat gaccatgttt ttggtttatc ggcagctcag
     6061 cgttatggcg gtatttttgt gcctccgcat attgcggtca tccatcaata tatgcgtgag
     6121 atgatggctg gcgggggcaa aatgatcctc gggtcagaca gccacacccg ttacggtgca
     6181 ttagggacaa tggcagtcgg tgagggtggc ggtgagttgg taaaacagct gcttaatgac
     6241 acctgggata tcgactatcc gggcgtggtt gcggtgcatc tgaccggaaa acctgcgccg
     6301 tatgtggggc cacaggatgt ggcgctggct atcattggcg cggtgttcaa aaacggttac
     6361 gtcaaaaaca aagtcatgga gttcgttgga ccgggcgtta gcgcgctctc taccgatttc
     6421 cgtaacagcg ttgacgtgat gaccacggaa accacctgtt taagctccgt ctggcaaact
     6481 gatgaagaag tgcataactg gctggcgctg cacggtcgcg gtcaggatta ctgccagctt
     6541 aaccctcaac cgatggcgta ctacgatggc tgcatcagcg ttgatttaag cgccatcaaa
     6601 ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg aaatcgacac actgaaccag
     6661 aacctgaccg acattctgcg tgagattgaa attgagtccg aacgcgtggc gcacggtaaa
     6721 gccaaactct cgctgctgga taaagtggaa aatggtcgcc tgaaagtgca gcaggggatt
     6781 atcgcgggct gttctggcgg taactacgaa aacgtcatcg cggcggcgaa tgcactgcgc
     6841 ggtcaatcct gtggcaatga caccttctcg ctggcggttt acccgtcatc acagccggtg
     6901 tttatggatc tcgccaaaaa aggtgtggta gcagatttga ttggcgcagg cgcaatcatc
     6961 agaaccgcat tctgtggccc atgctttggc gcgggcgata cgccaatcaa caacggtttg
     7021 agtattcgcc acaccacgcg taacttcccg aaccgcgaag gctctaagcc agccaatggg
     7081 cagatgtcag cggtggcgtt gatggacgct cgttctatcg ctgcgactgc ggcaaacggt
     7141 ggctatttaa cctctgccag cgaacttgat tgctgggaca acgtgccgga gtacgccttc
     7201 gatgtaacgc cgtataaaaa ccgtgtttat cagggctttg tgaaaggggc aactcagcaa
     7261 ccgctgattt acggaccgaa cattaaagac tggccggaat tgggtgcgct gactgacaat
     7321 atcgtcctga aagtgtgctc gaagatcctc gacgaagtca ccaccaccga cgaactgatt
     7381 ccttccggtg aaacctcttc ttatcgttca aatccgattg gtctggcggg gaatgtcagc
     7441 gagctgacag aggtgtttgc gcgcattaag cagattgctg gtcaggagca tattgatccg
     7501 ctgcaaactg aaattggcag catggtctat gcggtgaaac caggcgatgg ttctgcgcgt
     7561 gaacaggcgg cgagctgcca gcgtgtgatt ggcggtctgg cgaatattgc cgaggagtac
     7621 gcgactaaac gctaccgttc taacgtcatc aactggggga tgttaccgct gcagatggcg
     7681 gaagtgccaa cctttgaagt gggggattac atttacatcc ctggcattaa agcggcgctg
     7741 gataatccgg gtacgacgtt taaaggttat gtgatccatg aagatgcgcc ggtaacggaa
     7801 attacgcttt acatggaaag cctgacggct gaagagcgcg agattatcaa ggcgggtagt
     7861 ttgattaact tcaataaaaa ccgtcagatg taaaaagcgc catgtgaatg taggtcgcat
     7921 tcggcactta ttgtcggatg cgatgctggc gcatcttatc cgacctacaa atcgcatcga
     7981 accgtaggcc ggataaggcg tttacgccgc atccggcaaa tagttaatag ctcttacttc
     8041 ttctcctctg caaccacttt gctgcccacg ccgcggttat tgtattccca catgcggttg
     8101 tagttagtgt cattcagatt gcgctgtatt tcgtcgttat catctacgct gccggtatta
     8161 cccgcaaacg gacgattaga gatcaccgca tcggcccacg gtttagccgt gttaaaacct
     8221 tcgttgatgg cgctatcacg gatcaccacc tgaccgttgg tattagcatc aacatccagc
     8281 gagcggccca gttgcgccac gccatcaccg gaagcattga aacggctgtt tacggcgagg
     8341 aaaccgtaat agatgttaga cagcgtagcc ggtgcaaaca catatgcttc ttgctgggta
     8401 cgtgagttca ccacgcggaa ttcggtgtta tcgaacacca ctgcgccgcg accagaaacg
     8461 atatccacat ccccttcaat atagctgttg gtcaccagcg tacgcggctg acggttggtt
     8521 tccagacggt tctgcacacc gctgttggtg acaaagaagg tgttctgacg accgagaatg
     8581 ttaacgttgt taatctgtac ttggtcaccg tcagtacgca gtgctaccgc cggatggtta
     8641 cctgcatcta cgctatcgcc cagcgtgttt tcgatggtca gattttgcag ttgcagtcca
     8701 ttgttttgtg accagaagac cgcagaacac agaacaccga tactgtcgct gcgtttgctc
     8761 tggcagctat cgtacatata ccacgctggt ttacctggca tatatttgcc gcgcgggttg
     8821 acgtcgtgac gccagtcggc agggctcatg cccccatcaa gggaaagccc aatcttcaca
     8881 tcaatcggtt tttcacctgt accgtacaga gtgattccac ctggagcagc agggacatac
     8941 accgttccct gatactcacc aggcatcacg gcaatatact ggcgcttgtt ggtgcgcttg
     9001 ataattgccg catctaccgc cgcctgaatc gtggtatgcg ttacaccttg agtacccgcc
     9061 gggccgacaa caaagtcagg ttgcgcaggc agggttatcg gggacggatt ccacgctgca
     9121 gcacctggtg tcagggatgc aaaatagtgt tgcgcatcga aattcagcgc ttcttttgcc
     9181 gacagaatcg ggcgagaaga ggtaccaggc gcagtttgat cagaaggacg ttgatcgggt
     9241 ggtgttgagc tacaggcggt cagcgtcacg ccaaaagcca atgccagcgc cagacgggaa
     9301 actgaaaatg tgttcacagg ttgctccggg ctatgaaata gaaaaatgaa tccgttgaag
     9361 cctgcttttt tatactaagt tggcattata aaaaagcatt gcttatcaat ttgttgcaac
     9421 gaacaggtca ttatcagtca aaataaaatc attatttgat ttcaattttg tcccactccc
     9481 tgcctctgtc atcacgatac tgtgatgcca tggtgtccga cttgtgcccg agaagatgtt
     9541 gagcaaactt atcgcttatc tgcttctcat agagtcttgc agacaaactg cgcaactcgt
     9601 gaaaggtagg tggatcccct tcgaaggaaa gacctgatgc ttttcgtgcg cgcataaaat
     9661 accttgatac tgtgccggat gaaagcggtt cacgacgagt agatgcaatt atggtttctc
     9721 cgccaagaat ctctttgcat ttatcaagtg tttccttcat tgatatcccg agagcatcaa
     9781 catgcaatgt tgtagggatg gcaattttta cgcctgtttt gctttgctcg acataaagat
     9841 atccatctac gatatcagac cacttcattt cgcataaatc accaactcgt tgcccggtaa
     9901 caacagccag ttccattgca agtctgagcc aacatggtga tgattctgct gcttgataaa
     9961 ttttcaggta ctcgtcagcc gtaagtcttg atctccttac ctctgatttt gctgcgcgag
    10021 tggcagcgac cgggtttgtt gttatatggc cttcagctat tgcctctcgg aatgcatcgc
    10081 tcagtgttga tctgattaac ttggctgatg ccgccttgcc ctcgtctatg tatccattga
    10141 gcattgccgc aatttctttt gtggtgatgt cttcaagtgg agcatcaggc agccccctcc
    10201 ttattgc
//
LOCUS       DAJTUI010000123         9906 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTUI010000123 DAJTUI010000000
VERSION     DAJTUI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.123"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(160..900)
                     /gene="pflA"
                     /locus_tag="OO464_003481"
     CDS             complement(160..900)
                     /gene="pflA"
                     /locus_tag="OO464_003481"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR6228497.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="OO464_003482"
     CDS             complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="OO464_003482"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6228498.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3429..4286)
                     /gene="focA"
                     /locus_tag="OO464_003483"
     CDS             complement(3429..4286)
                     /gene="focA"
                     /locus_tag="OO464_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6228499.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4693..6453)
                     /locus_tag="OO464_003484"
     CDS             complement(4693..6453)
                     /locus_tag="OO464_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6228500.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6583..7209
                     /locus_tag="OO464_003485"
                     /pseudo
     CDS             6583..7209
                     /locus_tag="OO464_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7408..8496
                     /gene="serC"
                     /locus_tag="OO464_003486"
     CDS             7408..8496
                     /gene="serC"
                     /locus_tag="OO464_003486"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6228501.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8567..9850
                     /gene="aroA"
                     /locus_tag="OO464_003487"
     CDS             8567..9850
                     /gene="aroA"
                     /locus_tag="OO464_003487"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6228502.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2521 a         2368 c         2483 g         2534 t
ORIGIN      
        1 ttcgatgggg aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat
       61 atggtagatg cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa
      121 aaagcggcaa ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc
      181 gtactgctca agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc
      241 atcgagtttg tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg
      301 gagaagttcg attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc
      361 cgaatcgtca tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt
      421 tttgttcgcc agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt
      481 ttggtggatc tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag
      541 cagttcatca atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg
      601 aatgccttct tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc
      661 ttcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac
      721 cacttccttc atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc
      781 gcggttatga caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat
      841 acctgggccg tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat
      901 tgcggtgttt ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta
      961 cgtcgagtct gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt
     1021 gctaaaaaag gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca
     1081 gtcaaatcta attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct
     1141 ttagtcagcg agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat
     1201 ttttccgggt tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg
     1261 tgctgaccac cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg
     1321 ttggtcttac gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag
     1381 gagataccat ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca
     1441 gcacctttct ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca
     1501 cgacgaccgt ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc
     1561 agaacagact gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg
     1621 aaacgttcta ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc
     1681 gggtattcgc cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta
     1741 actttcgcat atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca
     1801 cacgccatgg tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg
     1861 tacttgtcgt gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag
     1921 tccatgaagt gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc
     1981 ggttcagact tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg
     2041 tacagcatgg ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg
     2101 atcatcgggc ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc
     2161 aggtcatcgt tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc
     2221 ttgaagttca gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc
     2281 cccatagtgt acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca
     2341 tcgaggccca taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca
     2401 tcgtattccg gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc
     2461 atttcctgcg cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca
     2521 tccaggaagg tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca
     2581 gccaggtagc cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag
     2641 atgtcgtagc cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca
     2701 gcgatttctt cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc
     2761 tgtagagagg tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca
     2821 acgcgacggt agtcaccgat gatacggcca cggccataag catctggcag accggtcaga
     2881 acaccggatt tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg
     2941 tgagttttac ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac
     3001 gctttgcagg aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc
     3061 ggagcttcag tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg
     3121 tgagaggtga tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt
     3181 tccagtttaa caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca
     3241 gccaggaagg actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg
     3301 tttacttcat tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca
     3361 ttaagctcgg acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc
     3421 gacaaccatt aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc
     3481 aacaaaccac cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag
     3541 ttcatcacgg tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc
     3601 ggagatgcga agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg
     3661 tgctcaaaac cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc
     3721 atcaggctgc ggccagaata actcatccat actgccagac ataccatcag gtttgccagg
     3781 ataccaagac agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg
     3841 acgtttagtc cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt
     3901 acaaacagca gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac
     3961 tgacaccagg tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag
     4021 agatcggctc cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc
     4081 agttttgcca tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg
     4141 aatgcgattg agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt
     4201 ttcgttgctt tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa
     4261 agatcaaaag ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat
     4321 actaacaaag cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc
     4381 ccgtaactcg catggttttt atgcaaatac ggagtaacta tttgattatc caaaataaaa
     4441 ataaatttta aaaattaaca aatgagttga attttttccg catcctccgc taaaacagtt
     4501 aattaaaagg gagcatcagg cgaataaagt aacaatatcg atcgtattta ttaaatacaa
     4561 attaccgata tttaacctta taattacaat tattttatta atgcaaatat atgtaaagcg
     4621 gggcattaaa aaaacgcccc gtaatataac tcagactaat cattaagcct acattgcgta
     4681 ggctatttga ttttattttg cccagaatgc tgctttggcg cgctgcagct tttcgtaggc
     4741 cttcaacaac gactgatgtg cagcaaacgc gtgcagatcg ctatctaccg gttgcaggcc
     4801 gtaaaacgcc gcttcgccgc tcattgccgc actggcggct tctacggcat ctgcgccgta
     4861 catgcgaaca aacgcattca gatattgcag cggctggcga tcttcttcct gtgccagtaa
     4921 taacaacgtt tgcaggcagc gataatagtt ggcgcgttcc ggactaaata ctgatgagtt
     4981 aaactccatc gtccattcgg tccagaccag agcctgttcc agatcgccac cagccagcgc
     5041 cagcatggct tttaattcac cgatacgcag ggtgtaccaa ccgttatccg acccggtcgc
     5101 cagacccaac agctcacgca cgcgggtaaa gtcatcaaaa ccttcttcat ccagttgctc
     5161 gatgaggttc aggtaatctt ctttttccca ctcgctgcct ggtagcgaaa gaatcgtttc
     5221 acgtaaatgg ctgcccatac tgttattcgc gagccacaga tcttcagccg gataaatatc
     5281 ggacatgcca ggcacgataa tacggcaagc ataaacgccc agatgctcgt aatcggcaat
     5341 ataaacttct ttatcttctt tgttgaagat agccatcagc gtagcaaact cttcttccgt
     5401 ggtgccggag aaattccagt ccacaaacgg ataatcggca tcctgcttga acaggtccca
     5461 ggagattaaa ccgctggaat cgataaagtg cgtttcgagg ttggtatgtt cagcgacttc
     5521 ttcatcatcg aaggttggcg gagtaaacac atccaaatct ttcaggccac gaccttgcag
     5581 cagctcggtc acggtacgtt ccagtgctac gccaaaatca ggatgcgcac cgaaagaggc
     5641 aaagcaggtg ccgtttgcag gattaaacag taccacgcaa atcaccggat actggccgcc
     5701 aagggaacca tcatatgcga agatcgggaa accttctgtt tccagtgttt cgatcgcttc
     5761 aaccaccgct gggtaacgcg ccagcacgtc tgccgggatt tccggcaagc tgatgctttc
     5821 agcaataatg cggtttttca cgtagcgttc gaaaacttcg gacaacccct gaacgcgtgc
     5881 ttcgttgcgg gtattgccag cggacatacc gttagaaacg tacaggttac caatgatatt
     5941 catcggaata taaacggtct gattgtcgga ctggcgcgta aacggcagac cgcaaatacc
     6001 acgatcttcg ttaccggatt gtaggtcaat cagcatactg ccggtcaatt cattctccgg
     6061 atcgtaaaat gcgcgcagac ggtcatcgag cagcccttct ggcacatcgt cattttcgct
     6121 cagtgggaac catttttcgt tgggataatg cacaaatgga ccgttggcga tggtttcgcc
     6181 cagccagaaa tcagcaaaaa agtagttggt tgagagacgc tcgaaatatt caccgagtgc
     6241 agaagccagc gccgctttct tggttgcgcc tttaccgttg gtaaaacaca gtgcgcactc
     6301 tttgtcgcga atatgtacag accagacgtt aggcacggga ttcagccagg aggcctcttc
     6361 aatctgaaag ccgaggtctg aaagtttttg ctggaagcga gcgatggaat cttccagagc
     6421 ggcatctttg ccggggataa atgtttgcgt catgaaaatc actttagtcg tacggaaagc
     6481 gcgcaataat acgggtttta tctcaaaggc gctatcaccg ccgccatgcc gatgaactgt
     6541 tgactatgct tttagcggat aaaccacgtt aagagcataa aaatgaaggc gttcgatctc
     6601 caccgtatgg catttgataa agtgcctttt gatttccttg gcgaagttgc actacgtagt
     6661 ctttatacct ttgtactggt tttttgttcc tcaaaatgac cggaagacgc ggtgtgcgac
     6721 agatgtcgct gtttgaagtg ttaatcattc tcacgctggg ttcagcggcg ggagatgtgg
     6781 cgttttatga tgatgtgccg atggtgccgg tacttatcgt ctttatcact ctggcgttgt
     6841 tataccgcct ggtaatgtga tggcgagctg gcctggtcga aactcaataa ctccaacatg
     6901 acggaatttg agttctttat ggagctacga ttgcgtggcg tggagcagct ggggcaggta
     6961 cgtctggcga ttctcgaaac caacgggcaa atcagtgtct atttctttga agatgacaag
     7021 gtgaaaccgg gtttacttat tttacccagt gattgtacgc agcgttacaa agtggtgccg
     7081 gagtcggcgg actatgcctg cattcgttgt agtgaaatca ttcatatgaa cgcgggggaa
     7141 aaacaattat gtccgcgctg tgcaaatcca gaatggacga aggcaagtcg ggcaaaacgg
     7201 gtgacctgac agtaaaaaca tcggcttttt gctaataatc cgagagattc ttttgtgtga
     7261 tgcaagccac atttttgccc tcaacggttt tactcattgc gatgtgtgtc actgaatgat
     7321 aaaaccgata gccacaggaa taatgtatta cctgtggtcg caatcgattg accgcgggtt
     7381 aatagcaacg caacgtggtg aggggaaatg gctcaaatct tcaattttag ttctggtccg
     7441 gcaatgctac cggcagaggt gcttaaacag gctcaacagg aactgcgcga ctggaacggt
     7501 cttggtacgt cggtgatgga agtgagtcac cgtggcaaag agttcattca ggttgcagag
     7561 gaagccgaga aggattttcg cgatcttctt aatgtcccct ccaactacaa ggtattattc
     7621 tgccatggcg gtggtcgcgg tcagtttgct gcggtaccgc tgaatattct cggtgataaa
     7681 accaccgcag attatgttga tgccggttac tgggcggcaa gtgccattaa agaagcgaaa
     7741 aaatactgca cgcctaatgt ctttgacgcc aaagtgactg ttgatggtct gcgcgcggtt
     7801 aagccaatgc gtgaatggca actctctgat aatgcggctt atatgcatta ctgcccgaat
     7861 gaaaccatcg acggtatcgc catcgacgag acgccagact tcggcaaaga tgtggtggtc
     7921 gccgccgact tctcttcaac cattctttcc cgtccgattg acgtcagccg ttatggtgta
     7981 atttacgctg gcgcgcagaa aaatatcggc ccggctggcc tgacaatcgt catcgttcgt
     8041 gaagatttgc tgggcaaagc gaatatcgcg tgtccgtcga ttctggatta ttccatcctc
     8101 aacgataacg gctccatgtt taacacgccg ccgacatttg cctggtatct atctggtctg
     8161 gtctttaaat ggctgaaagc gaacggcggt gtagctgaaa tggataaaat caatcagcaa
     8221 aaagcagaac tgctatatgg ggtgattgat aacagcgatt tctaccgcaa tgacgtggcg
     8281 aaagctaacc gttcgcggat gaacgtgccg ttccagttgg cggacagtgc gcttgacaaa
     8341 ttgttccttg aagagtcttt tgctgcaggc cttcatgcac tgaaaggaca ccgtgtggtc
     8401 ggcggaatgc gcgcgtctat ttataatgcc atgccgctgg aaggcgttaa agcgctgaca
     8461 gacttcatgg ttgagttcga acgccgtcac ggttaatgcc gaaattttgc ttaatcccca
     8521 cagccagcct gtggggtttt tatttctgtt gtagagagtt gagttcatgg aatccctgac
     8581 gttacaaccc atcgctcgtg tcgatggcac tattaatctg cccggttcca agagcgtttc
     8641 taaccgcgct ttattgcttg cggcattagc acacggcaaa acagtattaa ccaatctgct
     8701 ggatagcgac gacgtgcgcc atatgctgaa tgcattaaca gcgttagggt taagctatac
     8761 gctttcagcc gatcgtacgc gttgcgaaat tatcggtaac ggcggtccat tacacgcaga
     8821 aggtgccctg gagttgttcc tcggtaacgc cggaacggca atgcgtccgc tggcggcagc
     8881 tctttgtctg gatagcaatg atattgtgct gaccggtgag ccgcgtatga aagagcgccc
     8941 gattggtcat ctggtggatg cgctgcgcct gggcggggcg aagatcactt acctggaaca
     9001 agaaaattat ccgccgttgc gtttacaggg cggctttact ggcggcaacg ttgacgttga
     9061 tggctccgtt tccagccaat tcctcaccgc actgttaatg actgcgcctc ttgcgccgga
     9121 agatacggtg attcgtatta aaggcgatct ggtttctaaa ccttatatcg acatcacact
     9181 caatctgatg aagacgtttg gtgttgaaat tgaaaatcag cactatcaac aatttgtcgt
     9241 aaaaggcggg cagtcttatc agtctccggg tacttatttg gtcgaaggcg atgcatcttc
     9301 ggcttcttac ttcctggcag cagcagcaat caaaggcggc actgtaaaag tgaccggtat
     9361 tggacgtaac agtatgcagg gtgatattcg ctttgctgat gtgctggaaa aaatgggcgc
     9421 gaccatttgc tggggcgatg attatatttc ctgcacgcgt ggtgaactga acgctattga
     9481 tatggatatg aaccatattc ctgatgcggc gatgaccatt gccacggcgg cgttatttgc
     9541 aaaaggcacc accacgctgc gcaatatcta taactggcgt gttaaagaga ccgatcgcct
     9601 gtttgcgatg gcaacagaac tgcgtaaagt cggcgcggaa gtggaagagg ggcacgatta
     9661 cattcgtatc actcctccgg aaaaactgaa ctttgccgag atcgcgacat acaatgatca
     9721 ccggatggcg atgtgtttct cgctggtggc gttgtcagat acaccagtga cgattcttga
     9781 tcccaaatgc acggccaaaa catttccgga ttatttcgag cagctggcgc ggattagcca
     9841 ggcagcctga atgaacaacg ggcaataaat agccaaatct ttctttatca aaacgtcggc
     9901 acattg
//
LOCUS       DAJTUI010000124         9797 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTUI010000124 DAJTUI010000000
VERSION     DAJTUI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.124"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            429..1547
                     /locus_tag="OO464_003488"
     CDS             429..1547
                     /locus_tag="OO464_003488"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6228503.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1613..1861
                     /locus_tag="OO464_003489"
     CDS             1613..1861
                     /locus_tag="OO464_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR6228504.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1926..2294
                     /locus_tag="OO464_003490"
     CDS             1926..2294
                     /locus_tag="OO464_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6228505.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2388..3041
                     /gene="nfsB"
                     /locus_tag="OO464_003491"
     CDS             2388..3041
                     /gene="nfsB"
                     /locus_tag="OO464_003491"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR6228506.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3149..4396
                     /gene="ybdG"
                     /locus_tag="OO464_003492"
     CDS             3149..4396
                     /gene="ybdG"
                     /locus_tag="OO464_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR6228507.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="OO464_003493"
     CDS             complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="OO464_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR6228508.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5941..9084)
                     /gene="cusA"
                     /locus_tag="OO464_003494"
     CDS             complement(5941..9084)
                     /gene="cusA"
                     /locus_tag="OO464_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR6228509.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9096..9614)
                     /gene="cusB"
                     /locus_tag="OO464_003495"
                     /pseudo
     CDS             complement(9096..9614)
                     /gene="cusB"
                     /locus_tag="OO464_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2400 a         2711 c         2377 g         2309 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagggttg atagaaatat
       61 ctcctggggc ttcatctttc cagacctcag agtaaacctg aaatcggaaa gcttcaggca
      121 cccgcagtta tcttaccctt cctcaacaaa atagctgttt atttatacag caaatcctgt
      181 ttcggacttc tcactcccca tttgatcaac tgaagctgaa cccgtttttt tctgcatgcc
      241 tcaatcgtca aaattcttcg ccaccagcca gctctgccat aacttgccac attgaaaaag
      301 tagccaaagt gcttcaggca ccatcagttc accatcacca aaaaaaagaa acattccaga
      361 acttccgcat ctcgcccagc cgctccgact tctatactga atagaaaacg tcaacttaag
      421 agaaacctat gccattaccc gattttcatg tttctgaacc ttttaccctc ggtattgaac
      481 tggaaatgca ggtggttaat ccgccgggct atgacttaag ccaggactct tcaatgctga
      541 ttgacgcggt taaaaataag atcacggccg gagaggtaaa gcacgatatc accgaaagta
      601 tgctggagct ggcgacggat gtttgccgtg atatcaacca ggctgccggg cagttttcag
      661 cgatgcagaa agtcgtattg caggcagccg cagaccatca tctggaaatt tgcggcggtg
      721 gcacgccccc gtttcagaaa tggcagcgtc aggaggtatg cgacaacgaa cgctatcaac
      781 gaacgctgga aaactttggc tatctcatcc agcaggcgac cgtttttggt cagcatgtcc
      841 atgttggctg tgccagtggc gatgacgcca tttatttgct gcacggcttg tcacggtttg
      901 tgccgcactt tattgccctt tccgccgcgt cgccatatat gcagggaacg gatacgcgtt
      961 ttgccccctc acgaccgaat attttttccg cctttcctga taatggcccg atgccgtggg
     1021 tcagtaactg gcaacaattt gaagccctgt ttcgctgtct gagttacacc acgatgatcg
     1081 acagcattaa agatctgcac tgggatattc gccccagtcc tcattttggc acggtggagg
     1141 ttcgggtgat ggataccccg ttaaccctta gccacgcggt aaatatggcg ggattaattc
     1201 aggccaccgc ccactggtta ctgacggaac gcccgttcaa acatcaggag aaagattacc
     1261 tgctgtataa attcaaccgt ttccaggcct gtcgctatgg tctggaaggc gtcattaccg
     1321 atccgcacac tggcgatcgt cgaccgctaa cggaagatac cttgcgattg ctggaaaaaa
     1381 tcgccccttc agcacataaa attggcgcat cgagcgcaat tgaagcactg catcgccagg
     1441 tcgtcagcgg tctgaatgaa gcgcagctga tgcgcgattt cgtcgccgat ggcggctcgc
     1501 tgattgggct ggtgaaaaag cattgtgaga tctgggccgg tgactaagcc gggaattgcc
     1561 ttacagaaga atatccctga caatggtgtt ttaacttaca tttaacaatg ttatgaaaca
     1621 ccctttagaa accttgacca ccgcagcagg cattttgctg atggctttcc tctcttgcct
     1681 gctgctgccc gcccccgcac tggggctgac gctggcacaa aaactggtga ccatgttcca
     1741 tctgatggat cttagccagc tttacacttt attgttttgt ctgtggtttt tagtgctggg
     1801 cgctattgag tattttgttc tgcgctttat ctggcgacgc tggttctcgc tggcggatta
     1861 aacgtcgatt atcgccgaac ttcgcggcat actttgctta ttctccttcg cctaaaggaa
     1921 cgtttatgga taagcaatca ctgcacgaaa cggcgaaacg cctagctctt gagttaccct
     1981 ttgtcgagct ttgctggcct tttggcccgg agttcgatgt ttttaaaatt ggcggcaaga
     2041 tttttatgct gtcgtcggag ctacgcggcg tcccctttat caatctgaag tccgatccac
     2101 aaaaatccct gttaaatcag caaatatacc caagcattaa gccagggtat cacatgaata
     2161 aaaagcactg gatttcggtg tattccggcg aggaaatctc cgaagcgtta cttcgcgatc
     2221 tgatcaacga ttcgtggaat ctggtggttg atggtctggc taaacgcgat caaaaaagag
     2281 tgcgtccagg ctaaagcggg aatctatagc gcatttttct cgcttaccat ttctcgttga
     2341 accttgtaat ctgctggcac gcaaaattac ttacacatgg agtctttatg gatatcattt
     2401 ctgtcgcctt aaagcgtcat tccactaagg catttgatgc cagcaaaaaa cttaccccgg
     2461 aacaggccga gcagatcaaa acgctactgc aatacagccc atccagcacc aactcccagc
     2521 cgtggcattt tattgttgcc agcacggaag aaggtaaagc gcgtgttgcc aaatccgctg
     2581 ccggtaatta tgtgttcaac aaacgtaaaa tacttgatgc ctcgcacgtc gtggtgttct
     2641 gtgcaaaaac cgcgatggac gatgcctggc tgaagctggt tgttgaccag gaagatgccg
     2701 atggccgctt tgccacgccg gaagcgaaag ccgcgaacga taaaggtcgc aagttcttcg
     2761 cctatatgca ccgtaaagat ctgcatgatg atgcagagtg gatggcaaaa caggtttatc
     2821 tcaacgtcgg taacttcctg ctcggcgtgg cggcgctggg tctggacgcg gtgcccatcg
     2881 aaggttttga cgccgcaatc ctcgatgcag aatttggtct gaaagagaaa ggctacacca
     2941 gtctggtggt tgttccggta ggtcatcaca gcgttgaaga ttttaacgct acgctgccga
     3001 aatctcgtct gccgcaaaac accaccttaa ccgaagtgta attctctctt gccgggcatc
     3061 tgcccggcta tttcccctca gattctcctg atttgcataa ccctgtttca gccgtcatca
     3121 taggctgctg ttgtataaag gagacgttat gcaggattta atatcccagg ttgaagattt
     3181 agcgggtatt gagatcgatc acaccacctc gatggtgatg attttcggta ttatttttct
     3241 gaccgccgtc gtggtgcata ttattttgca ttgggtggta ctgcggacct tcgaaaaacg
     3301 tgccatcgcc agttcacggc tttggttgca aatcattacc cagaataaac tcttccaccg
     3361 tttagctttt accctgcagg ggattatcgt caatattcag gcggtattct ggctgcaaaa
     3421 aggcaccgaa gcggcagata ttctgactac ctgcgcgcag ttgtggatca tgatgtatgc
     3481 gctgctttca gtcttctcgt tgctggatgt tattttgaat ctggcgcaga aattcccggc
     3541 ggcatctcag ttaccgctga aagggatatt tcaggggatt aaactgatcg gcgcaatcct
     3601 ggtcggcatt ttgatgatct cgctgctgat tggtcagtcg ccagcgattc taatcagcgg
     3661 tcttggtgca atggctgccg tgctgatgtt ggtatttaaa gatccgattc ttggtctggt
     3721 ggcaggtatt cagctttccg cgaacgatat gctgaaactg ggcgactggc tggaaatgcc
     3781 gaaatacggc gcggatggcg cggtgatcga tattgggtta accaccgtca aagtgcgtaa
     3841 ctgggacaat accattacca ctattcccac ctggtctctg gtttctgact cctttaaaaa
     3901 ctggagcggg atgtcagcat ctggcgggcg acgtattaag cgcagtatca gtattgatgt
     3961 caccagtatt cgttttcttg atgaagacga aatgcaacgt ctgaataaag cgcatttgtt
     4021 aaagccttat ttaaccagcc gccatcagga aattaatgag tggaatcgcc agcaaggttc
     4081 tacggagtcg gtattaaacc tgcgccgaat gaccaatatt ggaacctttc gtgcctatct
     4141 gaacgaatat ctgcgtaacc atccgcggat tcgtaaagat atgaccttaa tggtacgcca
     4201 actggctcca ggtgataacg gtttaccgct cgagatctac gcgtttacta acaccgtggt
     4261 gtggctggaa tatgaaagca ttcaggctga tatattcgat cacatatttg cgattgtcga
     4321 agagtttggt ctgcgacttc atcagtcgcc aaccggcaat gatattcgct ctctggcggg
     4381 tgcatttaag cagtaattaa aaaaaccgct ctcatcgaat ggatgagagc ggacatcgtt
     4441 ttgtcggatg tagcgtgaat gccttatttc cgacgcagcg ttttaaatgc cacaaacagg
     4501 aatacaatcc acaccggcag caggatcgct gacaagcgca tatcatccat cgtgcacatc
     4561 agcagcaaaa tcatgccgag gaaggcgatg caaagatagt tgccgaacgg atagagcagc
     4621 gccttaaact gtgtttcacg cccctgacgt cgcatcgccg cgcgaaaacg cagatgcgcc
     4681 aggcagatca taatccagtt caacagcaac gttgctacca ccagcgccat cagcagacca
     4741 aacgcttttt gcggcagcag atagttgatt aacaccacca gcgaagtgat cgctccggaa
     4801 agcatcagcg agttaatcgg cacaccgcga cggctgacgc gagtcaaaaa cttcggcgca
     4861 ttaccctgca cagaaaggcc aaacagcatg cggctgttag agtaaacccc gctgttatac
     4921 accgacagcg atgccactag aatgacgaag ttcagcgcag aagctaccac attgctgtcg
     4981 agattatgga aaatcatcac aaacgggcta ctgttggatt tcacttccac ccacggatag
     5041 agtgccagta aaaccaccag tgaaccgata taaaacagca ggatgcgata caccacctga
     5101 tttaccgctt ttggaatgct tttttccgga tcgcgcgctt cagcggctgt aatcccaatc
     5161 agctccagac cgccgaagga gaacataatt accgccagcg acaaaatcag cccattccag
     5221 ccggtggcga agaaaccacc gtagcgccag aggttgtcga tactggcttt ctcgccgccg
     5281 taaccagaaa acagcagcca caggccaaag ccgatcatac cgatgatcgc cagcacttta
     5341 atcagcgcaa accagaactc ggtttcgcca tataagcgca cgttcaccag attaacggcg
     5401 ttgatgataa taaagaaggc ggcagcccaa atccacgttg gaacatccgg gaaccagtac
     5461 tgcatataga tgcccgcagc ggtcagctct gccattccca ccagcacgaa cattacccag
     5521 tagttccagc cagagaggaa gcccgcaaac ggtccccagt atttataggc aaagtgggca
     5581 aatgaaccgg ataccggctc ttcgaccacc atctcgccaa gctggcgcat aatcaggaaa
     5641 gcgatgatcc cggcgacgcc gtagcccagc aatacagccg gacccgccat ctgaatcgcc
     5701 gggccaatgc caagaaacag accagtacca attgcgccac ccagcgcaat cagttgaata
     5761 tgacggttat gtaatccgcg atgaagcgtc ggctcttgat tcgacgcagt atcttccgat
     5821 acggttgacg cgtttttcac gcctttcccc tgtgtgtctt ttttgttgag gggcaccttt
     5881 taacatttag tgcccatcgt cgcaagacac aatccacacg gttaaacgag gtatcctgct
     5941 ttaattccgt acccgatgtc ggtgcagcca catcagctta tacgccgccg ggataataaa
     6001 cagcgacagc aaaggtgcgg tgatcatgcc gccaatcatc ggtgcggcaa tccggctcat
     6061 cacctctgaa ccagcccccg ttccccacag aatcggcagc agaccggcga taatcaccgc
     6121 caccgtcatc gctttcgggc gcacgcgcag gaccgcgccg tgatacaacg cctcatccag
     6181 cttctgctcg ctgaatgttt gtggattatt caacgacggc tcggcctcta tggcgtgacg
     6241 taaatacatc agcatcacca cgccaaattc ggcggcgacc ccggcaaggg caataaagcc
     6301 ggtacccgtc gccacagaaa gatgaaagcc catccaccac agtaaccaga tgccgcccac
     6361 cagcgcaaac ggtacgctgc tgataatcag caacgcttcg cccacccgac ggaatgccag
     6421 atacaacagc acgaagatga tcatcagtgt catcggcacc atgagtttca gcttatggtt
     6481 ggcgcgctcc agtagctcga actgcccgga gaatgccacg ctggtgcccg gttttaactg
     6541 cactttttca gctatcgctt tttgcaaatc gtgaaccacc gacaccatgt cacgatcgcg
     6601 ggcatcgata taaatccagc tcgtcgggcg cgcattctcg gttttcagca tcgacggtcc
     6661 ggtagagact ttaacgtcgg ccacgtctgc cagggtgatt tgctgcttca tcggcgtcag
     6721 gatcggcaac tggcgcagtg cctgcggact atcgcgccag ctttgcggat aacgcagatt
     6781 aattggataa cgggcaatcc cttccaccgt ttcgccaacc atcgccccgc ccaccgcaga
     6841 agtcacaaac aactgcacat ccgccaccgt cataccgtaa cgtgcggctt tttcacggtt
     6901 aatctcaacg ttgatatagc gcccaccttc cagccgctca gcaagcgcag aagctacgcc
     6961 tggcaccgtt cgcgccactt cttcaatttg ctcagccatc gcgtcgatat ccgccagcac
     7021 agtgccggaa actttaatgc cgatggggct tttaatgccg gttgagagca tatcgatacg
     7081 gttacgaatt ggcggcaccc acagattcgc cagccccggc agacgcacgg tgttatccag
     7141 ttcctcaatg attttgtcca ttgtcatgcc tggccgccac tgatcctgtg gcttaagctg
     7201 gatggtcgtt tctaccatct ccagcggagc agaatcggtg gcggtttccg ctttcccggt
     7261 tttgccaaat acccgcgcca cttcaggtac gctcataatt agcttgtcgg ttttttgcag
     7321 catactcgcc gcctctgctg cggaaatccc cggcagcgtc gatggcatat acaacaagtc
     7381 gccttcattg atctgcggta aaaattcccc gccaacttta ttgagcggcc agagaaccgt
     7441 cagcaccgaa agcgccgcca ccagcagcgt ggttttcggc cagtgcagta ctttcagcaa
     7501 cagcggatga taaacacgaa tcaaaaagcg attgagcgga ttactgcttt ccggcggaat
     7561 tttgccacgg atccagtaac ccatcaggat agggatcact acgatcgcca gcagcgccgc
     7621 acccgccatc gcatacgttt tggtgaacgc caacgggcca aacagacgcc cttcctgccc
     7681 ttccagggtg aagatcggga taaacgacaa cgtgataatc agcagactga taaacagcgc
     7741 cggccccact tcaacagacg catcggtgat cacctgccag cgcgttttat tatccagcgt
     7801 ggcgtcagga tgctggtgct gccactcttc cagctgttta tgcgcattct cgatcatgac
     7861 gatagcggca tcgaccatcg ccccgacggc aatcgcaatg ccacccagcg acataatatt
     7921 ggcattcagc ccctggaagg gcatgacaat aaaagcaata cacaacccca gcggcaacga
     7981 aataatcgcc accagcgccg agcgcacatg ccagagaaac ggcgcgcaga ctaccgccac
     8041 cacaataaac tcttccagca acttgccgct gaggttgtcg atggcccggt caatgagctg
     8101 gctgcggtcg tatgttgtaa ctatctccac gccttccggc agactacttt tcagcgtttc
     8161 cagtttgtcc ttcacggcgg cgatcacttc tcgggcgttt ttgccggatc gcaggatcac
     8221 caccccgccc gccacttcgc cttcgccgtt tagttcggca atgccccggc gcatcttcgg
     8281 gccaatctgg accttcgcaa catcgcgcag ataaacgggc acgccatttt cactggcttt
     8341 taaaacgatg tgattaaagt cgtcgagcgt ttgtagatag ccgctggcgc gcaccatata
     8401 ttctgcttcc gccagttcga ttgacgaacc gcccgcttcc tggtttgaag catccagcgc
     8461 gctttttact tcggcgagac tgatgccata ctgcgccagg cgctgaggat cgataaccac
     8521 ctgatactct ttcaccacac cgcctaccga cgccacttcc gcaacgtcag gaatggtttt
     8581 cagctcatat ttgagaaacc agtcctgcaa tgagcgtaaa tcggcaagat cgtgcttacc
     8641 gctgcgatcc accagtgcat attcatagat ccagccaaca cccgtggcat ctggtcccag
     8701 ctcggcgctg actcccgcag gcagcttacc ctgtacctgg ttgaggtact ccagcacccg
     8761 cgaacgcgcc cagtacggat cggtgccatc ttcgaaaatg acatacacat aagagtcgcc
     8821 aaactgcgag aaaccacgca cagtcttcgc gccaggcacc gacaacatgg tggtggttag
     8881 cggataagtc acctgatttt caacgatttg cggtgcctgg ccgggatagc tggttttaat
     8941 aatcacctgc acatcggaga gatccggcaa cgcatccact ggcgtattaa tgatggtcca
     9001 ggtgccccag atgctcagaa acaacgcgcc catcagcacc aaaaaacggt tcgccaccga
     9061 gcgacgaata atccattcaa tcattggtta ttccctcaat gcgcatgggt agcactttca
     9121 gagcgcatcc gctccagtgc gccagaaata ttggcttcgg aatcaatcag gaacaggccg
     9181 ccggaaacca ccttttcacc ttccgccaga ccagagcgta atgcggtgac gccttgcgat
     9241 gcctggaaaa cagcaacgcg tttcggtaca aagcgcccgt cggcatcaac ggtaatcacc
     9301 cgctgttcgt tgccggtatc aatcagcgcc tgtgacggaa tgagcagcat cggttcgctg
     9361 gcggtgttga gttgcaacca ggcgttcatg cccggtttta gcgcctcgtc ggcgttgtcg
     9421 acttccagac gcagctgcag cgtgcgggtc gcggcatcca cgccaggtag cagcgtccat
     9481 ttgcggatgg tgagtgtttt atccggtcgc gccggaacgg tgagggtaaa ctgcgaggca
     9541 tctttcacca gccaggcgat ggactccggg atcgcagcag tgacccacac cgggtccata
     9601 ccctgaattc gcgattacgc gcaatccccc tgagaaccgc catgaacaag ggtttcatga
     9661 tttgtgattt tagtttggta cgcaatttgg tacacaacac tattttcact tcagcgggta
     9721 gtcatcaaac tcaccataac gagcatcgtt gatgatgtat gtgatcacca caaatatcac
     9781 atctggacta gttccgt
//
LOCUS       DAJTUI010000125         9797 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTUI010000125 DAJTUI010000000
VERSION     DAJTUI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.125"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..885)
                     /gene="bglB"
                     /locus_tag="OO464_003496"
     CDS             complement(<1..885)
                     /gene="bglB"
                     /locus_tag="OO464_003496"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR6228510.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFI"
     gene            complement(904..2781)
                     /gene="bglF"
                     /locus_tag="OO464_003497"
     CDS             complement(904..2781)
                     /gene="bglF"
                     /locus_tag="OO464_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR6228511.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2915..3736)
                     /gene="bglG"
                     /locus_tag="OO464_003498"
     CDS             complement(2915..3736)
                     /gene="bglG"
                     /locus_tag="OO464_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR6228512.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4023..4748)
                     /gene="phoU"
                     /locus_tag="OO464_003499"
     CDS             complement(4023..4748)
                     /gene="phoU"
                     /locus_tag="OO464_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR6228513.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4763..5536)
                     /gene="pstB"
                     /locus_tag="OO464_003500"
     CDS             complement(4763..5536)
                     /gene="pstB"
                     /locus_tag="OO464_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6228514.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5628..6518)
                     /gene="pstA"
                     /locus_tag="OO464_003501"
     CDS             complement(5628..6518)
                     /gene="pstA"
                     /locus_tag="OO464_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6228515.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6518..7477)
                     /gene="pstC"
                     /locus_tag="OO464_003502"
     CDS             complement(6518..7477)
                     /gene="pstC"
                     /locus_tag="OO464_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6228516.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7564..8604)
                     /gene="pstS"
                     /locus_tag="OO464_003503"
     CDS             complement(7564..8604)
                     /gene="pstS"
                     /locus_tag="OO464_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6228517.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8852..>9797)
                     /locus_tag="OO464_003504"
     CDS             complement(8852..>9797)
                     /locus_tag="OO464_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6228518.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2524 a         2554 c         2268 g         2451 t
ORIGIN      
        1 gatgaaatct acagtatgtt ttaaatcttc tgcgtcactt tcagtcatct caatggtgat
       61 attgtggtcg cggaagaaac gctgcatata gccgggatat tggccacgcg cctgaacatc
      121 accaaagaac atccagcgcc ggttctcttc catggcctgc aacatatcct gtggctggca
      181 ggtgaggggg taaaccagcc caccgagaag catattgccg attttcgctt cggggatcag
      241 gctatgatag gctttaactg cccgcgcact ggcaaccagt tgatgatgga tcgcctgata
      301 aacttccgcc tcgccactct cttctgccag ccccacgccc gtgaatggcg cgtgtaacga
      361 catattgatt tcattaaacg tcagccataa cgctacttta tgttggtagc gggtaaagac
      421 cgtgcgggcg taatgctcga agtgatcgat gaccgcccga ttagcccaac cgccgtagtt
      481 tttcaccagc ccatatagca tttcgtaatg ggataacgtt accagcggct tgatccccgc
      541 ctgcgccatt tcatcaaaca gccgatcgta aaacgctaac cccgcttcat tcggttcggc
      601 ttcgtcgccc tgagggaaaa ttcgcgccca ggcaatggaa atacgcagac aggtgaagcc
      661 catctcggca aataacgcga tatcttccgg gtaacggtaa taaaaatcga tggcgacatc
      721 tttgatattc tctttcccca ggatgcgcgg ttccattttt cccattacgc catgaggctg
      781 taaatctgag gtcgtgatcc ctttgccatc ttccagccag gcaccttcca cctgattggc
      841 agctgttgcg ccaccccaaa gaaatgtttc tggaaatgct ttcataatta actcctttta
      901 tcgttagcga atgatggata acagcggttc acctgcgctt atctgcgccg tgccgtgggg
      961 taatacgtct gtaaaatcat cgctattact gattaatacc ggcgtcgtca gatcaaatcc
     1021 ggcctcgcga atagcaggga tatcaaaaga aatcagccga tcgcctgtat tgaccttgtc
     1081 acccacgttg acgtgagcgg aaaagaattt gccgtccagt tttacggtgt cgataccgac
     1141 atgaatcagg atctccacac catcatctga ctcaatgcca atggcgtgta atgtggcgaa
     1201 caacgaagca attcgacccg caaccggaga acgcacttca ccaaccgagg gcagaatggc
     1261 gataccttta cccaacaggc cactggcaaa cgtggtatca gcgacgtgaa tgagcggcac
     1321 aatctctccc gtcatcggtg aacagatacc gccctgctca ggtggtgtaa taacctctgg
     1381 tgttttctct tgcggggcac cctgcgctgg ctgacgttta ctggtgatga aatgaagcat
     1441 caacgtacca acaaatgcgc aacctatggc aatgacaccg ccaataacgc tggcccagac
     1501 ggtgaaatca atccccgttg acgggatggt ctgcatgaag gtgaaaatac ttggcaaacc
     1561 aaaggagtaa actttcgttt gcgcgtagcc aatgatggtg gcccccaatg ctccactgat
     1621 acaggcgata acaaaggggt acttacgcgg caggttgacg ccatataccg ctggttcggt
     1681 gataccaaac agacccgtca acgccgctga tcccgccacc acttttttct gcgcatcgcg
     1741 ttcgcagagg aagacgccga gcgccgcccc gacctgcgcc ataatggcgg gcattaacag
     1801 cgggatcatg gtgtcgtagc ccagcacggt gaagttattg atacacagcg gcaccaggcc
     1861 cctgtgcagt ccgaacatga cgaagatttg ccagaagccg cccattaccg cgcccgcaaa
     1921 tgcaggaacc gcctgataaa gccagagata accggcggca atcatttcgc ttatccaggt
     1981 tgatagctgc cccaccagca gaaaggtgac gggtgtgata accatcagac atagcaatgg
     2041 tgtgaagaaa tttttgattg ccgacggtaa ccacgcatta agtcggcgtt ccagaatgct
     2101 gcacaaccag gcagaaaaaa taatgggaat aaccgatgac gagtaattca acaatgtgac
     2161 cggaataccc aggaaatcca gccccggcgc atccgctttt tgcccgttct cgaaggcagt
     2221 cagaattaat ggatgcacta acgctccacc aatcaccatg gcagtaaatg gattaccgcc
     2281 gaagcgtttg cccgcggtgt atcccaggat tatcgggaag aacccaaaca aggcatcact
     2341 ggcgctgaat aaaattaaat aagtaccact ttgttcggtc gtccactgaa aagtgagcgc
     2401 cagagccagc atacctttca ggatcccggt tgccgccatc agaccgatca gaggcgtaaa
     2461 aatacctgaa ataacataaa caaagcggtt tagcagatta cctttctcat cattttccgg
     2521 tgcctgttgc gctttttcgc caaggcctgc cacgctgtta accgccagga agacatcggc
     2581 cacatggtta cctatgacca cctgaaactg accaccgctt tccaccacca taataatacc
     2641 gggggtcttt ttcagtacct cagcttgcgc tttgctttca tcctttaact taaaacgtaa
     2701 tcgcgttgcg caatgcatca gactcacaat gttatctgcg cccccgactc ctgcgactat
     2761 ttttctggct aactccgtca taacttgccc tctaccgctt tgcggcaaaa ctccaaaaaa
     2821 aaacctgaaa aaaacggcct gacgtgaatc aagcaatttt tttcaggttt tgcccgctta
     2881 gtgcggtaac aatcctttac tcagtaataa tatttcagtg ttctttgcgc acgcgctcta
     2941 tatttatggc taaaaacata atctctgcgg gtgaaatttt acgttgatac tgcaaaccaa
     3001 taaaaatggc gatccgttcc gcacattgcc atgcttgcgg gtaattttgt tttactgctt
     3061 gttgtaatga ttcatcacta tcgttaattg aagcatgttc aagaatacgc caggataaaa
     3121 acttcagatg tgtaaccagt cgctgataac tcaagctttc ttcctggtaa ttaaggctga
     3181 actgaaattt tattaattgc agcatttcgc gcattaactg cgtgacacct gcaacatcct
     3241 ccatatttcc gctcatttgg gcactgacca gatgcatggc aataaagccc acttcatctt
     3301 tcggtaactg cacgcccaac cgtttatcaa taatggttaa cgcttcttcc cctagctgga
     3361 actctttcgg gtaaagccgc tggatatccc acagcaacac gttttgctga aagcgtttaa
     3421 tcgcaaactg gcaatggtca gttagcgaga tataaatact gtcctgtaat tttcccaagc
     3481 gctcctgcgc taaagagata agacgatcac aggttgccat cacctcaaga ggaatatgac
     3541 ttaagagttc gcttaatcgc ccgttcagtt catgactgct caaggcatac tctttttcta
     3601 ttccacttga gttaattctt tcgccagcgc gtttttgaaa gccaattccg cgccccatga
     3661 cgactttttc ccgctgttga tcatcaataa ccaccacaac attattgttg agaattttgg
     3721 tgatttgcat gttcatagca aggacctttt ttataaacaa aaaaaaaccg acttcaccag
     3781 tattctctgg ttatgtcagg ttttgcctgc gaatgcagta acaatccagt catttattaa
     3841 tggtttttat aacgaacatc caggttcgga aattaattta attgcgtgca gagaaaatag
     3901 caatgcgcta ttgataaaaa tatgaccatg ctcgcagtta ttaaccttgt gtaattttag
     3961 gaatttataa agttatatat aacaaatccc aataattaag ttattgggat ttgtctggtg
     4021 aattatttgt cgctatcttt tcccgccagc agtttatcca gctcatcacc gcctacatga
     4081 cggaaatcct gacccttcac gtagtagaag ataaactcac aaatattctg gcagcggtcg
     4141 ccgatacgtt cgattgaacg cgcacagaac agcgcggtaa gtacgctcgg aatagtgcgc
     4201 gaatcttcca tcatgtaggt catcagttga cgaacaatac cttcgtattc ctgatcgact
     4261 tttttatctt cacgataaat acgtaccgct tcgtcaatgt ccatccgcgc gaacgcgtcc
     4321 agcacgtcgt gcagcatctg gatggtatga cggcccagcg actccagact taccagcaac
     4381 ggctgatgct gctgggagaa tttctccagc gcagtacggc agattttgtc cgccacgtcg
     4441 ccaatacgct ccagctcggc aatggtttta ctgatcacca taaccagtcg caggtcgctc
     4501 gccgtcggct gacgtttggc gataatgcgc acgcaggctt catcgatcgc cacttccatc
     4561 atgttgacgt tcttgtcgcc ttcgatcacg cgcttcgcca gatcgctgtc ctggttatgc
     4621 atcgcggtga ttgcatcaga aagctgctgc tccaccatgc cgcccatggt catcacctgc
     4681 gtgcggatac tttccagttc ggcgttgaac tggccggaaa tatgtttatt aagattgaga
     4741 ctgtccataa cgcactcctg aatcaaccgt aacgaccggt gatgtagtct tctgtttgtt
     4801 tcttcgctgg cttggtgaac agatcgtccg tgttgctgaa ctcaatcaat tcgcccaggt
     4861 acataaatgc cgtgtggtcg gaacaacgcg cagcctgctg catgttgtgg gtgacgatca
     4921 ccacggtgta atcctgcttc agttcggtga tcagctcttc aatacgcccg gtagagatgg
     4981 ggtcgagcgc cgaacacggt tcgtcgagca gcagcacttc cgggcgaatg gcgataccac
     5041 gcgcaataca cagacgctgt tgctgaccac cagagagaga gtaaccgctc tggtgcaatt
     5101 tatctttggt ttcgttccac aatgcggctt tggtcaatgc ccactgcacg cgctcgtcca
     5161 tgtcggcacg ggagagcttc tcaaacagac gaacgccaaa ggcgatgttg tcgtagatgg
     5221 acatcgggaa cggcgtcggt ttctggaaca ccatgcccac tttcgcacgc agcagggcga
     5281 tatcctgaga gttggtcagg atgttgtcgc catcaagcag aatttcacct tccgcacgct
     5341 gctccgggta cagttcaaac attttgttga aggtacgcag cagcgtcgat ttaccgcagc
     5401 cggacggccc gataaacgcc gtaacctggt ttttggcgat atccaggttg atgtttttca
     5461 gggcatggaa tttgccgtag tagaagttca aattacgaac ctgaatttta ctcggggcag
     5521 tttcaaccat actcattgca atcttttcct catcgtacct gatgcttttc gctaatcagg
     5581 cttaccgtag ggccgggtaa ggcgttcacg ccgcatccgg aaaagtgtta accgtgttta
     5641 ttcttcgcaa aaacaacgcg cgccagaatg ttcagcagca ggacgcacag ggtaatgatc
     5701 aataccccgg cccaggccag ttgttgccat tccgcaaacg ggctcatcgc aaacttaaag
     5761 atcgtcaccg gcaggttggc gatcggctgc atcatgtccg tgctccagaa ctggttggag
     5821 agcgcggtaa acagcagcgg cgcggtttca ccggcaatac gggcaatcgc cagcaggata
     5881 ccggtcataa tcccggacac cgacgctttc agcgtaatcg cggagatcat cttccacttc
     5941 ggtgtaccca gcgcataagc cgcttcacgc aggctgtccg gcaccagttt cagcatgttc
     6001 tcagtggtgc ggataacgat cggcacctgc aacagcgcca gcgcaattac gcccgcccag
     6061 ccggagaagt gctccatctg tgccaccacg atggtgtaga caaacagacc aaccacaatc
     6121 gacggcgcgg agagcagaat gtcgttaatg aagcgaatca cttctgccag ccaggattta
     6181 cgaccatatt ccgccagata aatccccgcc ataatgccca gcggcgtacc gaataccgtg
     6241 gcccacaaaa ttaacagccc gctacccgcc agggcgttcg ccagaccacc accttccgta
     6301 ttgggcggcg gcgtcatttc agtgaacagc gccagcgaca taccgtcgat accgcgagtg
     6361 atggtggaca ttaaaatcca gatcagccag aacaggccga aggccatcgt cgccatcgag
     6421 agcgtcagcg caatacggtt tttgaggcgg cgacgcgcct gcattttgcg gcgagattca
     6481 gccagcgccg cagtggtttg catttcaacc atagccatta gcgtgccccc tcattcttag
     6541 ccaggcgcat aatcataaac ttcgatgcgg cgaggacgat gaaggtaatc acaaacagga
     6601 tcaggcccag ttccatcagt gcggcaacgt gcagaccgga ttccgcttcc gcaaattcgt
     6661 tcgccagcgc agaggtgata ctattgcccg gcatgtacag cgaggcgctg tcgagctggt
     6721 aggtgttacc gatgataaag gtcaccgcca tggtttcacc gagcgcgcgg cccagcccca
     6781 gcatgatgcc gccgataaca ccatttttgg tgaacggaag aacgatacgc cagataactt
     6841 cccaggtggt gcagccaata ccgtaggccg actctttcat catcaccggg gtttgttcga
     6901 acacatcacg cattaccgcc gcaatgtacg gaataatcat gatggcgagg atcacgcctg
     6961 ccgcgaggat accgatacca aatgcggggc cagagaacag cgcgccaaca atcgggatat
     7021 tcgacatgat attgccgacc ggctcctgaa agtaaacggc gaacagcggc gcaaagataa
     7081 acaggcccca catgccgtaa acgatacttg gaatggctgc cagcagctca atggcgatac
     7141 ccagcgggcg tttcagccag ccaggcgcaa gctcagtcag gaacagggcg ataccgaaac
     7201 tcaccgggac ggcgatcagc agcgcgataa acgaagtcac caacgtaccg tagatcggca
     7261 ccagcgcccc gtagatatcg ttcggtgcat cccactcttt ggtccatagg aaagccagac
     7321 caaatttctg aatgctcggc caggaggaga tgatcagaga gacaataatg ccacccaaca
     7381 tcaatagcac aatcagcgcc gccagtttta ccagcacgct gaaaattatg tcgccctttt
     7441 tacccggtgg gttaaaagcg ggcttggttg cagccataag ttactcttca gttaaacgcg
     7501 tttacaaagt cattttgtaa tgccggatgc ggcgtaaaac accgtacccg gcctggagtt
     7561 ttattagtac agcggcttac cgctactgtc tttaatattg gtcttccacg cagcgcgaac
     7621 ctgttcaact acactatccg gcaggctggc gtaatccagg tcgttcgcct gtttagcccc
     7681 ggttttgtac gcccagtcga agaatttcag cacctctgtg ccttgttctg gtttcttctg
     7741 atctttgtgg atcagaatga acgtggtaga ggtaataggc catgcatctt cgcctttctg
     7801 gttggtcaga tcctgagcga aggttttgct ccagtctgca ccttttgctg cattagcgaa
     7861 gttttcttcg gtcggactaa ccggtttacc atcagcggag atcagtttgg tgtacgccag
     7921 gttgttctgc ttcgcgtaag catattcaac ataaccaatt gcacccggca gacgctgaac
     7981 gaacgcggcg ataccgtcgt tacctttacc gcccagaccg atcggccatt ttacggtaga
     8041 gccagtacca acgttgtttt tccactcttc gttcactttc gccaggtagc tggtgaagac
     8101 gaaggaagtc ccggagccat ctgcgcggcg tactacagca atgttttgtg aaggcagttt
     8161 cagacccgga ttcagtttgg cgatggcttc atcatcccac ttcttgattt tgcccaggta
     8221 gatgtcgccg agggttttac catccagcac cagttcgcca gacttcagcc ctggaatgtt
     8281 aaccgccagc accacgccgc caatcacggt cgggaactgg aacagacctt cctgagccag
     8341 tttttcgtca gacagcggcg cgtcagaggc accaaaatca acggtattag cgataatctg
     8401 ttttacgcca ccggaagaac cgataccctg gtagttaact ttattaccgg tttctttctg
     8461 gtaagtgtca gcccatttgg catacaccgg cgcagggaag gttgcacctg cacctgtcag
     8521 gcttgcttct gcaaacacag agaaagcact catcgataag gtcgcggcga caacagttgc
     8581 gacggtggta cgcataactt tcataatgtc tcctgggagg attcataaag cattgtttgt
     8641 tggctacgag aagcaaaata ggacaaacag gtgacagtta tatataagga atatgacatt
     8701 tttatgacag aaaggaatta ccaaataact tactgatttt aaatgtgaag atgaaaattc
     8761 cacatgccat ctttttgtca tgatgcattt aaaagtgata aagaaatggg tgagatgaca
     8821 acgatgtgat gaacaatcgt tgtcagacct attagcgcac aatctcaaca gttatcgtcg
     8881 ctgttgcagt aaattgcccc ggggctgggc gagctccagt aaggctcgca ggagcagctg
     8941 aaaaagttac actacctgat ttttcaccat acactgttga tttatccatg tctgttggca
     9001 actcaccacc gttcactttg atttccttat tatttttgtc gtaaattttt atccccacgt
     9061 tagagtttga agttacgacc attgaattgt ccgtattggg ttcggtagca taaaaagacg
     9121 tactgatatc atcggtattc agtatcccca ggttttcaca ctcaatagtg actgttttag
     9181 taattttatg tgttgttacc gcacttccaa caacagatga aaaatctgca gctggtattt
     9241 ctttaaaatc aaaacaaact ggctttaaat tatttatttc gcaactttgc ggagctgtaa
     9301 tatcgccccg aatataaaca tcagccatcg gctgtgagta atccaccggg gtgtcacggc
     9361 taattgttcc atacaatgtc actatctttg tttcaggaat aatcaatttt cctgcaaatg
     9421 tcttcttgat ataaaaatat actatcgctt tagccccact ggatatactt aaaggttcaa
     9481 tgcggttgca attgtacttt gtattaccag acgggttgtt agcatgatat tcaaaaggtg
     9541 cattcacata cccaacccca agaatatcta tagcaatccc aacatcaagg ttcggattaa
     9601 gatcataata attaatatta gagtgaaccc cagcataagt taatgcctgg tttatttttg
     9661 acgtataata gatatagggg aattctccta cgttaccagt atcacaatga cagcgtccaa
     9721 gatatgagga tccattagtt acagtggtgt taaatgtcgt tccaacctta ttttcgttcg
     9781 cggtaataag atatccc
//
LOCUS       DAJTUI010000126         9696 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTUI010000126 DAJTUI010000000
VERSION     DAJTUI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.126"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(41..229)
                     /locus_tag="OO464_003505"
     CDS             complement(41..229)
                     /locus_tag="OO464_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6228519.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(448..1425)
                     /gene="epmA"
                     /locus_tag="OO464_003506"
     CDS             complement(448..1425)
                     /gene="epmA"
                     /locus_tag="OO464_003506"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6228520.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1750..3558
                     /gene="frdA"
                     /locus_tag="OO464_003507"
     CDS             1750..3558
                     /gene="frdA"
                     /locus_tag="OO464_003507"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6228521.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3551..4285
                     /gene="frdB"
                     /locus_tag="OO464_003508"
     CDS             3551..4285
                     /gene="frdB"
                     /locus_tag="OO464_003508"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6228522.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4296..4691
                     /gene="frdC"
                     /locus_tag="OO464_003509"
     CDS             4296..4691
                     /gene="frdC"
                     /locus_tag="OO464_003509"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6228523.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4702..5061
                     /gene="frdD"
                     /locus_tag="OO464_003510"
     CDS             4702..5061
                     /gene="frdD"
                     /locus_tag="OO464_003510"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6228524.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5124..6257
                     /locus_tag="OO464_003511"
     CDS             5124..6257
                     /locus_tag="OO464_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6228525.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6346..6879
                     /gene="blc"
                     /locus_tag="OO464_003512"
     CDS             6346..6879
                     /gene="blc"
                     /locus_tag="OO464_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6228526.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6876..7193)
                     /gene="sugE"
                     /locus_tag="OO464_003513"
     CDS             complement(6876..7193)
                     /gene="sugE"
                     /locus_tag="OO464_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6228527.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7369..7515)
                     /gene="ecnB"
                     /locus_tag="OO464_003514"
     CDS             complement(7369..7515)
                     /gene="ecnB"
                     /locus_tag="OO464_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6228528.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7626..7751)
                     /gene="ecnA"
                     /locus_tag="OO464_003515"
     CDS             complement(7626..7751)
                     /gene="ecnA"
                     /locus_tag="OO464_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6228529.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7803..8369)
                     /gene="efp"
                     /locus_tag="OO464_003516"
     CDS             complement(7803..8369)
                     /gene="efp"
                     /locus_tag="OO464_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6228530.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8411..9439
                     /gene="epmB"
                     /locus_tag="OO464_003517"
     CDS             8411..9439
                     /gene="epmB"
                     /locus_tag="OO464_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6228531.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2471 a         2483 c         2469 g         2273 t
ORIGIN      
        1 atgacaaggt catcaggcat tatctgaaca taaaacacta tcagtaagtt ggagtcatta
       61 ccattaaggc gaatggaata aagaataaca atgcagaaaa tatataaaag ggaatcgcac
      121 tataacccat taagtaataa gccgatgggc tattggcaaa tccaaatacg gaagtaaaga
      181 tcatcaatat gagtcctatc agactcatct tttttatcgt gtgaggcatg aaaccatcct
      241 tcatttcagc gcgtgtataa cacgtgccca ctgaactgca ctgcgcaaca ttgagcaggc
      301 agcgtaaaaa ccaatacaga attgatactt ggatgatatc aaatatccag tcttaaattg
      361 tggcctttaa cggaaaaatg aaaacatatg ctgcagaatg gcgcttatca cgccattctt
      421 cgctgttaat tcagtaattt ttcagaatta tgcccggtca acgctaaagg cgatgacttc
      481 agccagtgtc tccgcgccca gcgccaacat caccagacga tcaacaccta atgccacgcc
      541 ggaacagtca ggcataccga ctttcaaggc ttcaatcaga ttctggtcaa tggggtgctg
      601 cggcaaaccg cgcgccgcgc gcttacggtt atcttgttca aagcgttgtt gctgctcacg
      661 ggcatccgtt aattcatgga aaccattcgc cagctcaata cctttataat aaacctcaaa
      721 gcgttcagcg acccgatgat cttcggtact gatttgcgcc agtgatgcct ggctggctgg
      781 aaagtggtac acaaaggtcg gtttttcttt accaatattt ggctctacgc caaaggtaaa
      841 cagcaattgt agcaacgtgt cgcggtcttc ttcggtatca gcaacattgc tcaaatccag
      901 tttcgctgcg acttcccgca gttgcgtttt gtcggcagag agcgggtcaa tttccagata
      961 acgcaagaaa gcttgttgat aagaaaggct ttctgctgcc gggcagtcca gcacctgttg
     1021 taagagatcg tccacctcgt tcatcaaccg gtacatatca tagtgcggtc gataccactc
     1081 cagcatagtg aactcagggt tgtgataacg ccccatctct tcattacgga agctgcggca
     1141 cagctggaat accggcccac aaccagcaac cagcaggcgt ttcatatggt attccgggct
     1201 ggtcattaac cagagattca tcccctgcga atgcccgggg ccaacgaaac gtgtctcaaa
     1261 cgggaccaaa tgaatatcgg ttaccgtcgc ctggctcata caaggcgtct ccacctccag
     1321 cactccacga tcggcaaaga aacgacggat ctccgccata atcgccgcgc gttttaataa
     1381 gttaggaatg gatgcgctcg gctgccagga tgccgtttcg ctcatagtta aatctccagt
     1441 ttttgacaag ggcacgaagt ctactcgcaa cgcgacggcg agacaaattt tacgcaggaa
     1501 tcaaacagcg gtgggcagtg actaaaaaaa gcacgatctg atggtttagt aattaaatta
     1561 atcatcttca gtgataattt agccctcttg cgcactaaaa aaatcgatct cgtcaaattt
     1621 cagacttatc catcagacta tactgttgta cctataaagg agcagtggaa tagcgttcgc
     1681 agaccgtaac tttcaggcac ttaccctgaa gtacggggct gtgggataaa aacaatctgg
     1741 aggaatgtcg tgcaaacctt tcaagccgat cttgccattg taggcgccgg tggcgcggga
     1801 ttacgtgctg caattgctgc cgcgcaggca aatccaaatg caaaaatcgc actaatctca
     1861 aaagtatacc cgatgcgtag ccataccgtt gctgcagaag ggggctccgc cgctgtcgcg
     1921 caggatcatg acagcttcga atatcacttt cacgatacag tagcgggtgg cgactggttg
     1981 tgtgagcagg atgtcgtgga ttatttcgtc caccactgcc caaccgaaat gacccaactg
     2041 gaactgtggg gatgcccatg gagccgtcgc ccggatggta gcgtcaacgt acgtcgcttc
     2101 ggcggcatga aaatcgagcg cacctggttc gccgccgata agaccggctt ccatatgctg
     2161 cacacgctgt tccagacctc tctgcaattc ccgcagatcc agcgttttga cgaacatttc
     2221 gtgctggata ttctggttga tgatggtcat gttcgcggcc tggtagcaat aaacatgatg
     2281 gaaggcacgt tggtgcagat ccgtgctaac gcggtcgtta tggctaccgg cggtgcgggt
     2341 cgcgtttatc gttacaacac caacggcggc atcgttaccg gtgacggtat gggtatggcg
     2401 ctaagccacg gcgttccgct gcgtgacatg gaattcgttc agtatcaccc aaccggtctg
     2461 ccaggttccg gtatcctgat gaccgaaggc tgccgtggtg aaggcggtat tctggtcaac
     2521 aaaaatggct accgttatct gcaagattac ggcatgggcc cggaaactcc gctgggcgag
     2581 ccgaaaaaca aatatatgga actgggtcca cgcgacaaag tttctcaggc cttctggcac
     2641 gaatggcgta aaggcaacac catctccacg ccgcgtggtg atgtggttta tctcgacctg
     2701 cgtcacctcg gcgagaaaaa actgcatgaa cgtctgccgt tcatctgcga actggcgaaa
     2761 gcgtacgttg gcgtcgatcc ggttaaagaa ccgattccgg tacgtccgac tgcacactac
     2821 accatgggcg gtatcgaaac cgatcagaac tgtgaaaccc gcattaaagg tctgttcgcc
     2881 gtgggtgaat gttcctctgt tggtctgcac ggtgcaaacc gtctgggctc caactccctg
     2941 gcggaactgg tggtcttcgg ccgtctggcc ggtgaacaag cgacagagcg tgcagcaact
     3001 gccggtaatg gcaacgaagc ggcaattgaa gcgcaggcag ctggcgttga acaacgtctg
     3061 aaagatctag ttaaccagga tggcggcgaa aactgggcga agatccgcga cgaaatgggc
     3121 ctggcaatgg aagaaggttg cggtatctac cgtacgccgg aactgatgca gaaaaccatc
     3181 gacaagctgg cagagctgca ggaacgcttc aagcgcgtgc gcatcaccga cacctccagc
     3241 gtgttcaaca ccgacctgct ctacaccatt gaactgagcc acggtctgaa cgttgctgaa
     3301 tgtatggcgc actccgcaat ggcacgtaaa gagtcccgcg gcgcacacca gcgtctggac
     3361 gaaggttgca ccgagcgtga cgacgtcaac tttctcaaac acaccctcgc cttccgcgat
     3421 gctgatggca cgactcgcct ggagtacagc gacgtgaaga ttactacgct gccgccagct
     3481 aaacgcgttt acggtggcga agcggatgca gccgataagg cggaagcagc caataataag
     3541 gagaaggcga atggctgaga tgaaaaacct gaaaattgag gtggtgcgct ataacccgga
     3601 agtcgatacc gcaccgcata gcgcattcta tgaagtgcct tatgacgcaa ctacctcatt
     3661 actggatgcg ctgggctaca tcaaagacaa cctggcaccg gacctgagtt accgctggtc
     3721 ctgccgtatg gcgatttgtg gttcctgcgg catgatggtt aacaacgtgc caaaactggc
     3781 atgtaaaacc ttcctgcgtg attacaccga cggtatgaag gttgaagcgt tagctaactt
     3841 cccgattgaa cgcgatctgg tggtcgatat gacccacttc atcgaaagtc tggaagcgat
     3901 caaaccgtac atcatcggca actcccgcac cgcggatcag ggtactaaca tccagacccc
     3961 ggcgcagatg gcaaagtatc accagttctc cggttgcatc aactgtggtc tgtgctacgc
     4021 cgcgtgcccg cagtttggcc tgaacccaga gttcatcggt ccggctgcca ttacgctggc
     4081 gcatcgttat aacgaagata gccgcgacca cggtaagaag gagcgtatgg cgcagttgaa
     4141 cagccagaac ggtgtatgga gctgtacttt cgtgggctat tgctccgaag tctgcccgaa
     4201 acacgtcgat ccggctgcgg ccattcagca gggcaaagta gaaagttcga aagactttct
     4261 tatcgcgacc ctgaaaccac gctaaggagt gcaacatgac gactaaacgt aaaccgtatg
     4321 tacggccaat gacgtccacc tggtggaaaa aattgccgtt ttatcgcttt tacatgctgc
     4381 gcgaaggcac ggcggttccg gctgtgtggt tcagcattga actgattttc gcgctgtttg
     4441 ccctgaaaaa tggcccggaa gcctgggcgg gattcgtcga ctttttacaa aacccggtta
     4501 tcgtgatcat taacctgatc actctgacgg cagctctgct gcacaccaaa acctggtttg
     4561 aactggcacc gaaagcggcc aatatcattg taaaagacga aaaaatggga ccagagccaa
     4621 ttatcaaaag tctctgggcg gtaactgtgg ttgccaccat cgtaatcctg tttgttgccc
     4681 tgtactggta aggagcctga gatgattaat ccaaatccaa agcgttctga cgaaccggta
     4741 ttctggggcc tcttcggggc cggtggtatg tggagcgcaa ttattgcgcc ggtgatgatc
     4801 ctgctggtgg gtattctgct gccactgggg ctgtttccgg gtgatgcgct gagctacgag
     4861 cgcgttctgg cgttcgcgca gagcttcatt ggtcgcgtat tcctgttcct gatgatcgtt
     4921 ctgccgctgt ggtgtggttt acaccgtatg caccacgcga tgcacgatct gaaaatccac
     4981 gtgcctgcgg gcaaatgggt tttctacggt ctggctgcta tcctgacagt tgtcacgctg
     5041 attggtgtcg ttacaatcta acgcatcgcc aatgtaaatc cggcccgcct atggcgggcc
     5101 gttttgtatg gaaaccagac cttatgttca aaacgacgct ctgcgcctta ttaattaccg
     5161 cctcttactc cacatttgct gcccctcaac aaatcaacga tattgtgcat cgcacaatta
     5221 ccccgcttat agagcaacaa aagatccccg gtatggcggt ggcggtaatt tatcagggta
     5281 aaccttatta ctttacctgg ggctatgcgg acatcgccaa aaagcagccc gtcacacagc
     5341 aaacgttgtt tgagttaggt tcggtcagca aaacatttac gggcgtgctt ggtggcgacg
     5401 ctattgctcg aggggaaatc aagttaagcg atcccgcaac aaaatactgg cctgaactta
     5461 ccgctaaaca gtggaatggg atcacactat tacatcttgc gacctacacc gctggcggcc
     5521 tgccattgca ggtgccggat gaagtgaaat cctcaagcga cttgctgcgc ttctatcaaa
     5581 actggcagcc tgcatgggct ccaggaacac aacgtctgta tgccaactcc agtatcggtt
     5641 tgttcggcgc actggctgtg aagccgtctg gtttgagttt tgagcaggcg atgcaaactc
     5701 gtgtcttcca gccactcaaa ctcaaccata cgtggattaa tgtacctccc ccagaagaaa
     5761 agaattacgc ctggggatat cgcgaaggta aggcagtgca tgtttcgcca ggggcgttag
     5821 atgctgaagc ttatggtgtg aagtcgacca ttgaagatat ggcccgctgg gtgcaaagca
     5881 atttaccccc ccttgatatc aatgagaaaa cgcttcaaca agggatacaa ctggcacaat
     5941 ctcgctactg gcaaaccggc gatatgtatc agggcctggg ctgggaaatg ctggactggc
     6001 cggtcaatcc tgacagcatc attaacggca gtgacaataa aattgcactg gcagcacgcc
     6061 cggtaaaagc gattaagccc ccaactcctg cagtacgcgc atcatgggta cataaaacgg
     6121 gggcgaccgg cggatttggt agctatgtcg cgtttattcc agaaaaagag ctgggtatcg
     6181 tgatgctggc taacaaaaac tatcccaatc cagcgagagt cgccgccgcc tgtcagattc
     6241 tcaacgctct acagtaaaat ttcatcgggt ccgaattttc ggaccttttc tgcgcttttc
     6301 cttgctgtca tctacactta gaaaaaaacc agtaaggaaa catttatgcg cctgctccct
     6361 ctcgttgccg cagcgacagc tgcatttctg gtcgttgcct gtagttttcc tacgccgccg
     6421 cgtggcgtga ccgtagtgaa taatttcgac gccaaacgtt atctcggtac ctggtatgag
     6481 attgcccgtt ttgatcaccg ttttgaacgt ggactggaaa aagtcaccgc aacatacagc
     6541 ctgcgtgatg acggcggcct gaatgtcatt aataaaggct ataaccctga cagaggaatg
     6601 tggcagcaga gtgaagggaa agcgtacttt accggcgcac caactcgcgc tgcgctgaaa
     6661 gtgtcattct ttggtccttt ctatggcggt tataacgtta ttgcactcga tcgggaatac
     6721 cgccatgcgc tggtttgcgg cccggaccgc gactacctgt ggatactctc ccgcacgcca
     6781 accatttctg acgaagtgaa acaggagatg ctggcagtcg cgacccggga agggtttgat
     6841 gtcagtaagt ttatttgggt acagcagccc ggtagttagt gagtgctgag tttcagacca
     6901 ataatcccca atacgattaa cgccagactc gccagacgca tcgggttagc ggactcaccg
     6961 agcagcacaa tgccggtgat ggccgcgccg actgcgccaa tacccgtcca cacggcataa
     7021 gccgtcccta ccggtaacga tttcatcgcc caggcaagta gcgccatact gactatcatc
     7081 gccgtcacgg taataacact cggcgtcaaa cggctaaagc cgtgggtata tttcaggcca
     7141 acggcccata ccacttccag cagaccagca ataactaaga taatccagga catatcaggc
     7201 tccggaaatt tggggccgtc cccggtgaaa agaggcgttt gcaggtcgtc ctgcaaagct
     7261 gatggttgaa cggatatttt gccaggtggc aggggaattt caactattac ctttatgacg
     7321 atcgcagaaa aaacaaacgg cacgacactg ctgtcgtacc gttattgctt attgctgcgc
     7381 tttcgttgct gcgccagaaa tcgcgttacc gccatcagaa atgtcttcac caacgccacg
     7441 cgtggtgttg caggcagtta atactgttga aagcaccaga acagaaaaga tcgctgcaat
     7501 tgtcttcttc accataacgt cttcctttta ggtaatgtta tttatgtttg cctatagcaa
     7561 attaataata gacaacatcg ccaaaaatga tggaaatctg atgatttttc ggaagagaag
     7621 aaaaattagc tggcagcgcg agagatggag ttaccgagat gtttgatgtc ttcgccgaaa
     7681 ccgcaagcgg tgttacagcc cgtgagcagc gtgctggcaa gcaaaacaag aacgataagg
     7741 cgtttcatca tctctgacct gaataagtga tggtgcagcc tgcaggccgc accacaaccg
     7801 cattacttca cgcgagagac gtattcacca gagcgggtat ccactttgat gacttcgccg
     7861 atttgtacga acagcggaac tttaaccaca gcgccagtag acagggtagc cggtttgccg
     7921 ccagtacctg cggtatcacc tttcaggccc ggatcggtat caacgatttc cagttcaacg
     7981 aagttcggcg gagtaacgga gatcggctga ccattccaca gagttacgat acactctgcc
     8041 tgatccagca gccatttagc attgtcgcca attgcttttg catcagcaga cagctgctcg
     8101 aaagtttcgt tgttcatgaa gtgccagaac tcaccgtcgt tgtacaggta agtcaggttc
     8161 atatcgacaa catcagcgcc ttcagcggaa tcagtagatt tgaaggtttt ttctacgcga
     8221 gtaccggtca gcagacgacg cagtttaacg cgagcaaatg cctggccttt acccggtttt
     8281 acgaattcac tcgcttcaac cgcgtaaggt tcgccgtcta acatgatttt aagaccagca
     8341 cgaaaatcgt tgctatagta cgttgccata aggccctctg aaatttgtta attggtagct
     8401 aagccacaaa atggcgcata ttgtaaccct aaatacccca tccagagaag attggttaac
     8461 gcaacttgcc gatgttgtga ccgatcctga tgaacttctg cgtcttttga atatagacgc
     8521 ggacgaaaaa ctgttggccg gacgcagcgc caaaaagctg tttgccctgc gtgtgccccg
     8581 ctcatttatc gatcgcatgg agaaaggcaa tccggacgat cctcttttgc gtcaggtact
     8641 tacctcgcaa gatgagtttg tcgtcgcgtc cggattctcc accgacccac tggaagaaca
     8701 gcacagcgta gtgcctggtt tgttgcataa ataccacaac cgggcgcttt tgctggtcaa
     8761 aggcggctgc gcggtaaatt gccgctattg cttccgtcgc cacttcccct atgccgaaaa
     8821 tcagggcaac aagcgtaact ggcagactgc acttgagtat gttgctgcgc atccggaact
     8881 ggacgagatg attttctccg gcggcgatcc gctgatggcg aaagagcacg agctggactg
     8941 gttgctcaca caactggaag ccatcccgca tatcaaacgt ctgcggattc acagccgtct
     9001 gccgattgtg atcccggcac gtatcaccga ggcgctggtt gaacgctttg cccgttctac
     9061 gctgcaaatc ttgctggtga atcacatcaa tcatgccaat gaggtagatg aaacgttccg
     9121 tcaggcgatg gctaaattgc gccgtgtcgg tgtcaccctg cttaaccaga gcgttctgtt
     9181 acgtggtgtg aacgataacg cacaaacgtt ggcaaacctg agtaatgcgt tgttcgatgc
     9241 cggcgtgatg ccgtattacc tgcatgtgct agataaagta cagggcgcgg cgcattttat
     9301 ggtgagtgat gacgaagcac ggcagattat gcgtgagttg ctgacattgg tgtcgggata
     9361 tctggtgccg aaactggcgc gagaaattgg cggcgaaccc agcaaaacgc cgctggatct
     9421 ccagctacgc cagcagtaac tgattaaaca gggaagttaa acattaaaat tgcctgatgc
     9481 gctacgctta tcaggcctac atcgtgcatt gaaattgaat ggatttgcaa tgaactgtag
     9541 gccggataag gcgtttacgc cgcatccggc aagagcaaca aacgcaagta cagcaaccac
     9601 tgagaaattt aatctttgca cgttgcataa cctttaatta ttcatatgca ttgctgttgt
     9661 tactttttat atagctaaca ccaataatta accttt
//
LOCUS       DAJTUI010000127         9686 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTUI010000127 DAJTUI010000000
VERSION     DAJTUI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.127"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            50..244
                     /locus_tag="OO464_003518"
     CDS             50..244
                     /locus_tag="OO464_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR6228532.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
     gene            complement(494..1396)
                     /locus_tag="OO464_003519"
                     /pseudo
     CDS             complement(494..1396)
                     /locus_tag="OO464_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1456..1734
                     /locus_tag="OO464_003520"
     CDS             1456..1734
                     /locus_tag="OO464_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228533.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1735..2262)
                     /gene="gadE"
                     /locus_tag="OO464_003521"
     CDS             complement(1735..2262)
                     /gene="gadE"
                     /locus_tag="OO464_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6228534.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(3061..3633)
                     /gene="hdeD"
                     /locus_tag="OO464_003522"
     CDS             complement(3061..3633)
                     /gene="hdeD"
                     /locus_tag="OO464_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6228535.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3888..4220
                     /gene="hdeA"
                     /locus_tag="OO464_003523"
     CDS             3888..4220
                     /gene="hdeA"
                     /locus_tag="OO464_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6228536.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            4336..4662
                     /gene="hdeB"
                     /locus_tag="OO464_003524"
     CDS             4336..4662
                     /gene="hdeB"
                     /locus_tag="OO464_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6228537.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4726..5372
                     /locus_tag="OO464_003525"
                     /pseudo
     CDS             4726..5372
                     /locus_tag="OO464_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(5414..5944)
                     /locus_tag="OO464_003526"
     CDS             complement(5414..5944)
                     /locus_tag="OO464_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6228538.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(6100..6666)
                     /gene="slp"
                     /locus_tag="OO464_003527"
     CDS             complement(6100..6666)
                     /gene="slp"
                     /locus_tag="OO464_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6228539.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6913..8136)
                     /locus_tag="OO464_003528"
     CDS             complement(6913..8136)
                     /locus_tag="OO464_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228540.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8717..>9686)
                     /locus_tag="OO464_003529"
     CDS             complement(8717..>9686)
                     /locus_tag="OO464_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6228541.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGSDNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2825 a         1922 c         2032 g         2907 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat aaatacattc tggcggctac cttcatccac taatgccgtg acatacatgc
      541 caggcagcaa gtcaccattt gggttgggga aaatcgcccg taacgtcacg gagcccgtgg
      601 tttcatcaac cgtcgggtcg gagaatttca gcgtgccggt ctggctgtaa cgtttaccat
      661 tttccagatt gagctgtact ggcgtactgc cctgaacctg tttgatttgc ccactggcga
      721 cctcttcttt catgcgtaag aaatcttgca ccgactgcgt gagatcgaca taaatcgggt
      781 ccagacgttg tacggtaacc agcgaatctg cctgattagc ggtaacgagt gcgccgacgg
      841 tcaccgacga tttcccgctg acgcccgtaa tcggcgaggt gacattcgcg tattgcagat
      901 tgatcgtcgc ctgttcaaca gccgctttgg cgacggtgac attggcttct gcttcattca
      961 gctgggtgcg cgcggtgtcg taatcctgac ggctaacgta gttggtcttc agcaacgatg
     1021 cctggcggtt aaaggtgatg cgggcattgc tggcggtaga gagcgctttc gccagcgagc
     1081 ctttggcgga gtttagctcg gcctgtaaag gtgcaggatc aatctgatac agcgaatcgc
     1141 cctggttcac tttatcgcct tcgataaagt tgcgtttaat gataataccg cccacctggg
     1201 gacgtatctc ggcaacttca taaggaacgg ttctaccggg caattcgctc aacacattga
     1261 ccgaaccagg ggagagtgtg acgacaccga cctcaggcgt catggcggcg gcgttttccg
     1321 ccgatttgtc atcgcaggcg gtgagcatcg cgccgcagaa taacaacggt attaacagct
     1381 ttcttcttct gttcatttta gtccctgaaa attcttgaga aataacggtt ctttaaattg
     1441 cgcgcgagga gagatatgag ggatttacag accagcggca tagtggggtt gtcagccagt
     1501 aaggtgggat acaggcacag tgatcgacat ggtgaggtca acgacaaaaa aaatgttgcc
     1561 gttctgccaa cagttcctgc cagcattcgg gcaacgaagg gttctactgg tggatacaca
     1621 taccagggga atcaagtcgt tttagcctct cctttagtaa tcaccggggg taatgaaatc
     1681 agcatctgca tccctcgtca tgccagccat caatttcagt tgcttatgtc ctgactaaaa
     1741 ataagatgtg atacccaggg tgacgatgtc gctcatacgt cttagtttca gcttgtacat
     1801 aatgttgaat ttctgaatct ttaatgtttg ttgcgaaata ttgagcgtcg acgtgatatt
     1861 gctttcattt ttatttttca gaatcagttg cataattttg aactgattat gtgaaaaaca
     1921 taattgtgaa ttcttatggg gcaagtgttt accataagta atctcaagaa gatttttgat
     1981 actttctttg cggctaacaa aataaatagg cgctttagct tttagtttac tgatgtgata
     2041 aggtgccaaa acaataattc tctcggcatc taatttctcc agaaatttaa tcgcttcttc
     2101 atcaaggata tgattgtgat aggtatcaat gataaccttg aagggtttat tgcctacttt
     2161 tttgatctct gacagggaat tgatttttat catttcgtga ttatctttca actgccaaaa
     2221 gccctgtaaa agaaaagaat ctttcgtcat gagaaaaatc ataacttgct ccttagccgt
     2281 tatcgtttcg aacatgtcat ccttttcatt atttacatcc ttgtccgaat cgttgttcaa
     2341 tatagtaaac gcctataaga actttgccct tcgtgaacta atacgtctat taacactaat
     2401 caatacttca gatttatcca tcacgcactc catatattga tcctaagtaa gattcctggt
     2461 tgttatcagc ttgtaaatag taaacaaaaa gttaacgttt gcggattact gcccaagaat
     2521 aagttatcaa taaccagttt gtgatctctg aagaatatta ctaaagttaa aatgtaatcc
     2581 gatttaaata tcgagtctcc ttgtttcgac ttaagctggc aattggattg ccagctttct
     2641 tttaacgcat tgaaattacc tgagtaaata acgcattcat gtcttcaggt ttttatatag
     2701 cgtctggaga cacggatatt tatgcaatgt aatcaacgcc agttgcaaaa aaatgaaccc
     2761 ggattactta tttaaccgcc catagcatca acgcttacgg aactctttgt tcactttccc
     2821 acagcacttt taaaacatta aacctgacta cggaaaatat cagccatgat aatgtttgaa
     2881 atggctaatt tgccatggag tgaaaaaaat tagatgaaat tcagtaggtt gaaataatca
     2941 ctagcaggta attatttcaa tgatagtgcg caattgatct acaacactgc gtagcggaga
     3001 gagtattaat cggatcatag tcacatcaag tgactatgat ccgggtgaca accggggtaa
     3061 ttattgctgc ttaacgaaca aactggcgaa gctgaacagg ctggcggcgc taaatatcag
     3121 ttcaattccc accagtgtgg aaaccagcgt tacagacacc atcggcgttg caccaaggaa
     3181 tatccaggca atgacgatat ccagcacacc aataacgagc tgtagccagc tgcctttcat
     3241 tgaacgctga cgataccaac tcatcaggcg aataacccct gcaacacaga acaaaccggc
     3301 aataaatgcc gcaatggcaa aaatgcccag ctccggtgca cggatgaaga aatagccgat
     3361 caataaatag gcgactgcga cgaggaaacc ggataatacc ggccagaaat tatgactgcg
     3421 gttgctgaat aacccgacaa taagcgcaat acccgagcag attaataatg cacccactac
     3481 tgtgcttaaa atatcgccag agacgaacgg gaaactgata cacagcaacc cgacgataaa
     3541 cagcagcacg gcaataaact ggattgctct gcgatgcttt ttcagcatct ccagatcaaa
     3601 cttcaaaatt gttgccttat ctatatataa catagaacca ccctataaaa ttaagaagaa
     3661 aatcccctgc tatcaatcta tgccaaaaac gcgtctaaga atgcagtcga tttaataaaa
     3721 atttcctaat tgcagtatct gatgcatctg taactcattg tattgaaata aaaatatctg
     3781 attttgatat tttccatcaa catgacatat acagaaaacc aggttataac ctcagtgtcg
     3841 aaattgattc gtgacggctc tttcacttta tagttgagga tattacgatg aaaaaagtat
     3901 taggcgttat tcttggtggt ctgcttcttc tgccagttgt gagcaatgca gcggatgcgc
     3961 aaaaagcagc tgataacaaa aaaccggtca actcatggac ctgtgaagat ttcctggctg
     4021 tggacgaatc cttccagcca actgcagttg gttttgctga agcgctgaac aacaaagata
     4081 aaccagaaga tgcggtttta gatgttcagg gtattgcaac cgtaacccca gctatcgttc
     4141 aggcttgtac tcaggataaa caagccaact ttaaagataa agttaaaggc gaatgggaca
     4201 aaattaagaa agatatgtaa ttccgggaat gcgttacatc gtacttcctt gcatattgaa
     4261 caggccggaa tatcttcttt aaaagcagct attcctcctg ttcatatata atctctatat
     4321 tgaatgggtt acaaaatgaa tatttcatct ctccgtaaag cgtttatttt tatgggcgct
     4381 gtagcggctt tgtcactggt gaacgcacaa tctgcgttgg cagccaatga atctgctaaa
     4441 gatatgacct gccaggaatt tattgatctg aatccaaaag caatgacccc ggttgcatgg
     4501 tggatgctgc atgaagaaac agtatataaa ggtggcgata ccgttacttt aaatgaaacc
     4561 gatctcactc aaattcctaa agtgatcgaa tactgtaaga aaaacccgca gaaaaatttg
     4621 tataccttca aaaatcaagc atctaatgac ttgccgaatt aatgaggtac aagtaaaaag
     4681 gagtagcaag ttgagccatc ttgctgctcc tttttgcatt tttatatgac agcagaattt
     4741 attatacgtc ttatactcgc ggcaattgcc tgtggcgcta ttggcatgga aaggcaaatg
     4801 cgcggcaaag gagcagggtt acgcacacat gtattaattg gcatgggaag cgccctgttt
     4861 atgattgttt cgaaatatgg ttttgctgac gtgctgtctt tagatcacgt cggactcgac
     4921 cccagccgta ttgctgctca ggtggtgacg ggcgtcgggt ttatcggtgc aggtaacatt
     4981 ctcgttcgta accaaaatat tgtaggtctg acgacggcag cggatatctg ggtgaccgcc
     5041 gcataggtat ggttattggc agcggtatgt acgaactggg tatttatggt tcagtgatga
     5101 ccttgttggt gctggaagtc ttccatcaat taaccttccg cctgatgaac aaaaattacc
     5161 atttacagct gacattagtg aacggtaata cggtatcaat gctcgactgg tttaagcagc
     5221 aaaaaataaa aactgattta gtttcgttgc aggaaaatga agatcatgaa gtggtcgcca
     5281 ttgatatcca gcttcatgcc acaacgttga tagaagacct gttgcgatta ttaaaaggta
     5341 tggcaggggt gaaaggcgtt tcaattagct aatctgtgtg gcagcgtagc cagactcacc
     5401 gtaagcctga aattcacacc agataatcaa tatgctgatg gcgtaccagc tcattgatcc
     5461 gcttaagatt gaggatcatc atgatattgt gtttatggca gtagaacgtt tttagcgata
     5521 ttttcagttt ctccagaata tcttcagttg tcatttgatt cgcaatgcag ctcagaacct
     5581 gatgttgctt gcgcgaaagt gacacattcg cttcaggcac agagcagtaa ctgccattga
     5641 tgagtgcgac aaaatcaatg atagtgatag tccgcggaac aaaggtcacc ggtactttcc
     5701 ccagacagcg tttaatatta aagggggaga aaacaatgac atggacaggt tttaatcgtt
     5761 caatttgagt gagaaaatta gaatgaaata cattattcat taatgtatca ataatgacga
     5821 aggcattctg atgcaacatt acgtcgatct cttcttcgtt ctgtatatga acgacattac
     5881 ctttactcag aatgtttttc atcgcggtga agaacatcgt atccctggta attataagaa
     5941 acataaccga ctcctggtgc ggacattaac tttataatca gtttgccaaa ggtaattaac
     6001 aggacagggc ttataataat aatagttagc ttactgtata ttaattttat gtaatacatg
     6061 atgcagatgc tgagaaagca tctgcatctt tcggtggtgt tatttgacca gctcaggtgt
     6121 tacctgactc accgcattgg tgtagtaagg cgcaccccag cccggttccg gccagaatgc
     6181 accatagccg taatcccaca ggttataggt ggtattaacc acttctctca aatgccacac
     6241 ctggatgccc tgcatattca cttccaggaa gttatacggg actttattga taaagcctgg
     6301 ttgttcaccc tgaatggtac cgaggatggt aacaaagtga ttacgatagt tcactggatc
     6361 aaggaagccg ctttgtctgg cgagcagtcg gccctgatag ttggcttcaa tatcaggctt
     6421 cgcatagcta tccaacggta atacagcgat ttctaacaac gtatccgttt tgccattgat
     6481 aacgttgata accttcccac caaagcgcgc ttgttgacca acatataacc ccggctggtt
     6541 atgaacagca acaaaacttt tttgaatatc aggttggtta ttgcctttga tattttgcgg
     6601 aattgaacta catgcggcaa gcaaaaatga aaggctgagg atgagtgcac cttttgtcat
     6661 gttcatgtta ctatccttat caacaaatat taaaaccata atagatattt ctttatagtt
     6721 ttcatctgat tctgaggtga tatgcctgat agtaacgtaa acctgtctga tgtttatatc
     6781 ccttctgttt agttcaattc cgttattttg gggcgtggta tgagtatcca tcgcccaata
     6841 atataaccct ttgttttaaa attacataaa atctattttt attcaatgat tataaagtgc
     6901 taataatcaa tattatggta ttatattctg ataatgattt gataagcgaa ttcttttgcc
     6961 agatggtatt tctatttcca ccccataatt tatatctttt tcattaggca gatctctctt
     7021 taaataaaag gggtatgaaa tcttttcggg ttgaagtgtc tcgtaatgaa attctatgaa
     7081 ttcattttta gacattgttg cccatttttg aattttattg tatagagagg gtgttgtaaa
     7141 ctcttgaagt tgtttataac atattttttt gaactcttgc ttatatttta atatgtcatc
     7201 aaatgaaaga ttcactacct ttgcctctaa gaaatcaatt attctcgaag caatttccgc
     7261 tttcgcgcgt aaagagttgt cattaatagt taacaatgta gaaaacaaac cattgatttg
     7321 tttgtcaaga tgttgttcat atttatgtag tttggggatg ggattttcct tgtgtgaaaa
     7381 aatagtgtta aataaatttg cgtcagtgaa atttatatta agtagatctt caaaataaaa
     7441 ttttgataag gttaggacta gcttggacat atcttgccct gtcatgttga aatcaataaa
     7501 gtcgcatcca gcaagggctt ccatcctatt ttcattttta ctgtgctcaa tatacagctt
     7561 cgccattttt aagtacaagc tttgaatgtc tcttatatca gttggtaagt cagctcgttg
     7621 cccagcaaaa tttttattat cggaagaaac ggtttggaca ctgaatcttt cgggtattcc
     7681 ttcttctacg agaattttag ttatgattgg attgttgggt tctggattaa cgacaacaat
     7741 gatttgacat acagaatcct tatccttttc gtatctaatg ttatgaatct gattaagtat
     7801 tgattgtgtc tgttgcgtaa gtggtgtcga acttatcgtt tcaatgttat taataagact
     7861 tacaagttga accacaactt ttgagtagtc tgcatttgta tatgtggtgt tcgtgtggca
     7921 tgcattaatt tgttggagtt gttgttgcgc taaatcaaat tgttcagggt tgtatccttc
     7981 tccagagaat gtggaattaa tgggttgttg ttcttcccaa ttatcacaag gccacttcgg
     8041 tgtgtctggc gttttaacat tatcggtatt tgttgtgcag ttataaatat cgccgtgata
     8101 tgtatttatt atacctgggg taaagtcaat tgacataaca ttccttattc atcatctgtt
     8161 ttatgctaat gtttttaaat tggataatta gaacaggaat ggattttctt ctacacacaa
     8221 taaatataat gcgttatatc ttcctgatat aattaccttt aaattccctt tcatttccac
     8281 ccggtaatat ttgtgtataa aaaaagggca gtgatagtat cgtaaaggaa aacgttaaca
     8341 actatcaaaa aacgctcatt ttaaaaagtt attatgttct ccgatagtgt gtgaagtaat
     8401 atttttgtga tgttttagat gtgtaataat gaaccaggtt aaagtgtatt taaaaatgtg
     8461 ttgtattagc gactgaaatc tgccttttaa aaaggaggag ggtactgaga caaagagtca
     8521 ttgcttctcc gataaaatgt cagagaagca tgattacgtg gttattgtct gttgatgact
     8581 tgttgaccga gccgtcatca ttctcaatgc attagttaaa tcatttgtga tttttaagga
     8641 ttatcctgtc tatgattttc tacttagaag gatatggcac taactatgca tcttatagtt
     8701 tactgctggc atctatttaa aatagcaatt gagtcaaata gcccatcaag atagccatac
     8761 ctaatataac cgtcaggaat gcagctatca tcggcattct gaacattgat ttaagtaata
     8821 tcacttccgt caggcttgcg ccggcactgc cgatgattaa tgccattaat gctcccagac
     8881 ccattccttt tgtcatcaga acagatgcca gagggataac cgcctcagcc cggatataca
     8941 gcggaatacc gacaacggcg cttaatggga tggcgaaggg attatctgat cctgcatgag
     9001 cggcaatcca cgctgaggga ataaagccat aaataaaaga tcctattaat actcctaaca
     9061 aaagatatgg cagtacatct ttaaactgtt gcaacgcatc tttggtggct agttttatgg
     9121 gattaatact tactacagct ttggtactgc aacaggtaac tacggggttc tcaatggctt
     9181 tagccgctgg actacaacaa ctcactttta tgggcgtata tgtcgttccc ggcgaagttt
     9241 ttgctggagc acaacaattt gctgatgagc ttttactggc aatgatatga cgttcaaacc
     9301 ccagagaatc caggataata ctggcaagaa cggagacgcc ggctgcgata atcgcgtaca
     9361 acaaggtgac tttccaacca aaggtcatcc acattagtcc gacgataatg ggattaagta
     9421 atggtgaaac aaataaaaaa gtgagagtcg gaccaaaccc ggctttcgct gacaacaatc
     9481 cacgtaacat gggtattgtc gagcaactac agaacggggt aacggctccc aacagagcag
     9541 ccaggagata gcctctccct tttctggccc ccatcatctg ctggatttta tggtctggca
     9601 ccttttgtct tatcaggctg acaccggcac taatcactat aaacagcaac gaaagctcga
     9661 cggcgagaaa cacaaacatc tctgcg
//
LOCUS       DAJTUI010000128         9494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTUI010000128 DAJTUI010000000
VERSION     DAJTUI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.128"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            112..1743
                     /gene="oppA"
                     /locus_tag="OO464_003530"
     CDS             112..1743
                     /gene="oppA"
                     /locus_tag="OO464_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6228542.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            1829..2749
                     /gene="oppB"
                     /locus_tag="OO464_003531"
     CDS             1829..2749
                     /gene="oppB"
                     /locus_tag="OO464_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6228543.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            2764..3672
                     /gene="oppC"
                     /locus_tag="OO464_003532"
     CDS             2764..3672
                     /gene="oppC"
                     /locus_tag="OO464_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6228544.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3684..4697
                     /gene="oppD"
                     /locus_tag="OO464_003533"
     CDS             3684..4697
                     /gene="oppD"
                     /locus_tag="OO464_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6228545.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4694..5698
                     /gene="oppF"
                     /locus_tag="OO464_003534"
     CDS             4694..5698
                     /gene="oppF"
                     /locus_tag="OO464_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6228546.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(5751..6080)
                     /locus_tag="OO464_003535"
     CDS             complement(5751..6080)
                     /locus_tag="OO464_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6228547.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6115..7575)
                     /gene="cls"
                     /locus_tag="OO464_003536"
     CDS             complement(6115..7575)
                     /gene="cls"
                     /locus_tag="OO464_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6228548.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7718..7891
                     /locus_tag="OO464_003537"
     CDS             7718..7891
                     /locus_tag="OO464_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6228549.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(7946..9199)
                     /gene="kch"
                     /locus_tag="OO464_003538"
     CDS             complement(7946..9199)
                     /gene="kch"
                     /locus_tag="OO464_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR6228550.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2464 a         2243 c         2367 g         2420 t
ORIGIN      
        1 aacgataaag taacctgaca gcagaaagtc tccgagcttg tgcagggtcc caatccggga
       61 ttacacatgc tggttaatac cagtaattat aatgagggag tccaaaaaac aatgaccaac
      121 atcaccaaga gaagtttagt agcagctggc gttctggctg cgctaatggc agggaatgtc
      181 gcgctggcag ctgatgtacc cgcaggcgtc acactggcgg aaaaacaaac actggtacgt
      241 aacaatggtt cagaagttca gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct
      301 aatatcagcc gagacctgtt tgaaggctta ctggtcagcg atcttgacgg tcatccagca
      361 cctggcgtcg ctgaatcctg ggataataaa gacgcgaaag tctggacctt ccatttgcgt
      421 aaagatgcga aatggtctga tggcacgcca gtcacagcac aagactttgt gtatagctgg
      481 caacgttctg ttgatccgaa cactgcttct ccgtatgcca gttatctgca atatgggcat
      541 atcgccggta ttgatgaaat tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa
      601 gctattgatg atcacacatt agaagtcacc ttaagtgaac ccgttccgta cttctataaa
      661 ttacttgttc acccatcaac ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa
      721 aaatggaccc agcctggtaa tatcgtcacc aacggtgcct ataccttaaa agattgggtc
      781 gtaaacgaac gaatcgttct tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt
      841 attaaccagg taacctattt gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt
      901 agtggtgaaa tcgacatgac gtataacaac atgccgatcg aattgttcca gaagctgaaa
      961 aaagagatcc cggacgaagt tcacgttgat ccatacctgt gcacttacta ttacgaaatt
     1021 aacaaccaga aaccgccatt caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg
     1081 gaccgcgata tcattgttaa taaagtgaaa gcgcagggcg acatgcccgc ctatggttat
     1141 actccaccgt atactgatgg cgcaaaattg actcagccag aatggtttgg ctggagccag
     1201 gaaaaacgta acgaagaagc gaaaaaactg ctggctgaag cgggttatac cgcagacaaa
     1261 ccgttgacca tcaacctgcg gtataacacc tccgatctgc ataaaaagct ggcgattgct
     1321 gcctcttcat tgtggaagaa aaacattggt gtaaacgtca aactggttaa ccaggagtgg
     1381 aaaacgttcc tcgacacccg tcaccagggc acttttgatg tggcccgtgc aggctggtgt
     1441 gcagactaca acgaaccaac ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat
     1501 accgcgcatt ataagagccc ggcctttgac agcattatgg cggaaacgct gaaagtgact
     1561 gacgaggcgc agcgcacagc tctgtacact aaagcagaac aacagctgga taaggattcg
     1621 gccattgttc ctgtttatta ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc
     1681 tataccggca aagatccgct ggataatacc tatacccgga atatgtacat tgtgaagcac
     1741 taatggcaat acgtggggca ggagtgtcct gctccacggt gtctgatttt tatcgcatta
     1801 cagaaggcac aggccagaag gtagggcaat gttaaaattt attctacgtc gttgtctgga
     1861 agcgattccg acgctattta ttcttattac tatttcgttc tttatgatgc gcctcgcacc
     1921 gggaagccct tttaccggcg aacgtacttt accgccagaa gtgatggcca atatcgaagc
     1981 gaaatatcat cttaatgatc cgatcatgac acagtatttc agctacctga aacaactggc
     2041 gcatggcgat ttcggtccat cgtttaaata taaagattat tcggtcaatg acctggtggc
     2101 atccagtttt cccgtttctg ccaaactggg agccgccgca tttttccttg cggtaatact
     2161 gggtgttagt gctggcgtta ttgccgcatt aaaacaaaac accaaatggg actataccgt
     2221 gatggggctg gcaatgaccg gggttgttat ccccagtttt gtggttgcgc cattattagt
     2281 catgatattt gcgatcattt tgcattggct gccgggcggt ggctggaatg gtggggcgct
     2341 taaattcatg atattgccga tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg
     2401 tattacccgt ggctctatga ttgaagtatt acactccaac tttattcgta ctgcccgggc
     2461 gaaagggtta cctatgcggc ggatcatttt acgccatgca ttaaaacctg ctctgttacc
     2521 cgtgctctcc tatatgggcc ccgcatttgt cggcattatt accggttcta tggtcatcga
     2581 aaccatttat ggtttgccgg ggattgggca actgttcgtt aatggcgcat tgaaccgtga
     2641 ctattcctta gtgttaagcc tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc
     2701 cattgtcgat gtgctatatg cggttatcga cccgaaaatc cgttactgat actggagctc
     2761 gcgatgatgt taagtaagaa aaacagcgag acgctggaaa atttcagtga aaagctggag
     2821 gtcgaagggc gcagcttgtg gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg
     2881 gttgccagtc tgatagtgct ggtgctgatc gcgttatttg taatcctggc accgatgctt
     2941 tcccagtttg cctatgacga tactgactgg gcgatgatgt ccagcgcccc ggatatggag
     3001 tccggtcact actttggtac tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt
     3061 ggcgggcgta tctcactcat ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg
     3121 acactttatg gttcgctttc cggttatctg ggcggtaaag tggattcggt aatgatgcgt
     3181 ctgctggaaa tcctcaactc cttcccattc atgttcttcg tcattttgct ggtgaccttt
     3241 ttcggccaaa acatcctgct gattttcgtg gcgattggca tggtttcctg gctggatatg
     3301 gctcgtattg tgcgtgggca aaccctgagt ctgaagcgca aagagtttat tgaggcggca
     3361 caagttggcg gtgtatcgac gccgggcatt gttattcgcc acattgtgcc gaacgtactc
     3421 ggtgtggtgg tggtctacgc atcgctactg gtgcccagca tgatcctctt tgaatctttc
     3481 cttagcttcc tggggttggg tacgcaagag ccgttaagca gctggggggc attgctgagt
     3541 gatggcgcga actcgatgga agtctctcca tggttactgt tgttcccagc gggattcctt
     3601 gtggtgacac tgttttgttt caactttatc ggcgatggct tgcgtgatgc cctcgacccg
     3661 aaagatcgtt aaggagtgca gccatgagcg taattgaaac tgcaactgtg ccgctcgcac
     3721 aacaacaggc tgacgcactg ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg
     3781 acggcgacgt cacggcggtc aatgatttaa atttttccct acgtgccgga gaaacgctgg
     3841 gcattgtagg tgagtctggt tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt
     3901 tggctgccaa cggacgcatt ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt
     3961 tgccagagcg tgaactcaat aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc
     4021 caatgacttc gttgaatccc tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc
     4081 tgcataagaa catgagcaaa gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg
     4141 taaaaatgcc ggaagcacgt aaacgcatga aaatgtaccc gcacgaattt tctggcggca
     4201 tgcgtcagcg agtcatgatt gcgatggcat tgctatgtcg acctaagttg ctgattgcgg
     4261 atgaacccac tacagcgctg gacgtcaccg tacaggcgca gatcatgacg ctattgaatg
     4321 aactgaagcg ggaatttaat actgccatca ttatgatcac ccacgatctt ggcgtggtgg
     4381 cggggatctg tgacaaagtg ctggtaatgt acgccgggcg cacgatggaa tatggcaatg
     4441 cgcgcgatgt cttttatcaa cccgttcatc cttattctat cggtttgctc aacgcggtgc
     4501 cgcgtcttga tgcggaaggt gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc
     4561 tgcgattacc gaaaggttgc ccgttccagc cacgttgtcc acatgcgatg gaaatttgta
     4621 gtagcgcacc gccgctggaa gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg
     4681 tggaggaact gttatgaatg ctgtaactga aggaagaaaa gtcctccttg aaatcgccga
     4741 tcttaaagtg cactttgaaa tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac
     4801 gctcaaagcc gtcgatggtg tcactcttcg cctgtatgaa ggggaaacat taggtgtggt
     4861 aggggaatcg ggatgcggta agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc
     4921 gaccgacggt catgttgcct ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg
     4981 gcgtgccgtt cgcagtgata ttcagatgat tttccaggat ccgttggcat cgctaaaccc
     5041 gcgtatgacc atcggcgaga tcatcgctga accactgcgt acttatcatc cgaaaatgtc
     5101 acgccaggaa gttcgcgagc gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa
     5161 cctgattaac cgctatccgc atgagttctc cggtgggcag tgccagcgta tcgggattgc
     5221 tcgtgctctt attcttgaac cgaagctgat tatctgcgat gagccggttt cggcgctgga
     5281 cgtgtcaatt caggcgcagg tggttaacct gctccagcag ctgcaacgtg agatgggatt
     5341 gtcattaatt tttattgctc atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt
     5401 ggtgatgtat ctcggccatg cagtagaact ggggacctat gatgaggtct accacaatcc
     5461 actacatcct tacaccaggg cattgatgtc ggcagtcccc atacctgatc cggatctgga
     5521 gaagaacaaa accatccagt tactggaagg ggaattaccg tcgccgatca acccgccttc
     5581 cggttgtgtt ttccgtaccc gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc
     5641 tgttctggag gggagtttca gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa
     5701 acaataaggg ctgacagttg tcagcccttt ttcacgctaa aagcgattat ttattcccgc
     5761 cagatgatat ggcaaagttt gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca
     5821 tcgctgattt caccatcttc actgtccgcc agaccaatca ccacttcggc aaaaaagtcc
     5881 gggttcaaat cgaaatcaac atgttcctgc cagtcttccg ccggatcgaa taactccgcg
     5941 ccgccgcgct cttcaaactg aagattgaac agcagaacat cagctggatc gagattgtcg
     6001 gcagccagtt cgagaaaaat atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga
     6061 ttgtttagat ccatatccat agtcactacc tgtttaacct ctgttggcga cgttttacag
     6121 caacgggctg aagaagtaaa acagtcgctc ggcgacacgt tgccataatg gacgttttag
     6181 ccataaacgg gcatcgagca gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag
     6241 gtctgcacca aaacctttat cgtcgattgc caaggtaatc tcgaaattca gccacagact
     6301 acgcatatca aggttaacgg tgccaaccag acttagttcg ccatcgacca gcacgctctt
     6361 ggtatgcagt aacccgcctt caaactgata aattttaacc ccagcagcaa gcagttccgt
     6421 aaagaatgcg cgactggccc agccgaccag catcgagtca ttttttcgcg gaaggataat
     6481 actgacatcc accccgcgct gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg
     6541 cacaaagtag ggcgtggtca tgatcaaata ttcatgcgcc gaataagccg cagtcaataa
     6601 tgcctggtga atgagatctt ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg
     6661 accgctggcc tgttcaaacg gcataatatt gacatctggt ggtggcggca gaatacgttt
     6721 tccggtttca atctcccagt cgcaggaata aataatcccc atcgcggtgg cgatggggcc
     6781 ttccatacgc gccatcagat caatccattg ccctacgccc gcatcttgtt tgaagtagcg
     6841 aggatcgacc atattcatgc tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt
     6901 gcgatgttgg cgcaggtcca tacggcgtaa aaacacacgc atcagattga cctttaaggc
     6961 ttcgaccact tcaataccgg cattacgcat tagctcgggc cacgggctgc ggaaaaaagc
     7021 cacactcccg gcggagtcga gcatcaatcg gcaatgaata ccacgccgtg cagccgccat
     7081 taatgattca gccacctggt ccgccatgcc gccgggctgc cagatataaa acaccatctc
     7141 aatattatgg cgcgcgagct ggatgtcgcg gattaacgcc tgcatcacat catctgactc
     7201 ggtcatcagc tgtagctgat tccctttgac cccagcgatc ccctgacgac gctcgcaaag
     7261 cttgaataat ggcgcagcga cactgctatt ttcttcggcg aagatatgct tacaggcttt
     7321 aaggtcgtta agccattttg cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg
     7381 tttgcctaaa tggagctcgc caacggcaag ataggcaata attccgacta acggcagaat
     7441 gtaaataatc aacagccagg ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg
     7501 taaagttacg cctgcaatga gcaaccagta tcccagaatg gccaaccaac tcaccaacgt
     7561 ataaacggtt gtcatagatt agaaatcctt ttgaaagcgc attgttatga gtttacgcat
     7621 atggattcat ctggcaaata aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg
     7681 tataatgacc attctgttat tgcatagagt agttaacatg aagcggagta gaacggaagt
     7741 ggggcgctgg cgcatgcagc gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca
     7801 atcgcgccga aatatgcgta tccacagcat caggaagtgc attctaaaca aacagcgtaa
     7861 ctcgttattg tttgcgatct acaatatcta aatgtactgg gcaccattgc ggtgcccaat
     7921 aagtttactc ttccgtcttt aaactctatt tttgcgccga ttctttactg tcagctttcg
     7981 tttcttgttc atcgttatcg ctgaaaatac catgacctga gttcaacaac attgaaacga
     8041 gcatatcatt attaatctct tcaccattta aaactcgcgc cagaatttcg ctgccaaaca
     8101 gttgcggtga gagaatgata tccggatgta ccatcttaat cttattcagg tttttgctat
     8161 cactgacggc gagaactgtt ttgacatcac tgctcatatc ttttgccgag agtacaacaa
     8221 acgcgttatc tgcatcgtta tcactcagcg ccagaatggc ccggcaacga tcgattcccg
     8281 ctttctttaa tactgaactg tcattactgt caccggggat aacatcagcg ttatcgccta
     8341 agcgttgctc aagttgcttg atatcatctt caggcaagtt gctgataacc gttacgtttt
     8401 gtccgcgttg attcagttgc aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa
     8461 taaaatgatc tttacgatgc attgtatgat tgtttccttt taaaagtttg ttgaatcccc
     8521 cgcggataag cggaccaaaa attgaggtca tagatgtggc aaaaacggta atgccggaaa
     8581 taatgaccga aatagtgaac aatcgtgctg attcagaaac agggacaata tctccatagc
     8641 cgacggttga catggtttct atcgaaaaat agaacgcggt catcaaactt tctattcgcg
     8701 gattaaaacc ttcgcttaaa taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga
     8761 aactaataaa tgcaaaaatt gtcccggctg cggcgctact gtgggagaag tctttgcgca
     8821 gtatcagcaa aaacaccagc gtaaaaatgc aaaatccaat actaaatttc agccagggat
     8881 aaaaatgcag ggtgtaaatt agcgctatca acaacaaaat gatactgatt gcccatgcga
     8941 gctttgcccg gaacagtaag ccaatggagt tcaggacaag aaagacgccg agcataaata
     9001 gtggggcgtt tgccagcaag gagagatcca actctgaaaa tgctttgata tggaaaatat
     9061 caagcaaatt gaccgacata ccatagattg ttttaaaaat aagcaatcca tttaaaaaga
     9121 cggccagcgc gagaatgtcg tgccgtaggg tcacccataa atttgttgct gtttgtttga
     9181 atgtagccca gtgactcact gatacctctg taaccacaat tttgtttaat tatcttagcg
     9241 tgcacatttt ctgaacatac atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt
     9301 atccgcagta atcaaaggtt ttgagtgagc aactctgcgt caggaaaggg cttttttcct
     9361 gaacatgctc acatcatata aagaaataca gataaagtta ttatctgctt gtggtagtga
     9421 atgcactgac cggctataag gaaaggccaa acaagaacac ggttgcaaaa accgtgccct
     9481 taaatattgg atct
//
LOCUS       DAJTUI010000129         9480 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTUI010000129 DAJTUI010000000
VERSION     DAJTUI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.129"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..179)
                     /locus_tag="OO464_003539"
     CDS             complement(<1..179)
                     /locus_tag="OO464_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228551.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLG"
     gene            699..1394
                     /gene="minC"
                     /locus_tag="OO464_003540"
     CDS             699..1394
                     /gene="minC"
                     /locus_tag="OO464_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6228552.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1418..2230
                     /gene="minD"
                     /locus_tag="OO464_003541"
     CDS             1418..2230
                     /gene="minD"
                     /locus_tag="OO464_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6228553.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2234..2500
                     /gene="minE"
                     /locus_tag="OO464_003542"
     CDS             2234..2500
                     /gene="minE"
                     /locus_tag="OO464_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6228554.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2872..3216)
                     /locus_tag="OO464_003543"
                     /pseudo
     CDS             complement(2872..3216)
                     /locus_tag="OO464_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3251..3370)
                     /locus_tag="OO464_003544"
     CDS             complement(3251..3370)
                     /locus_tag="OO464_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6228555.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3331..3516)
                     /locus_tag="OO464_003545"
     CDS             complement(3331..3516)
                     /locus_tag="OO464_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228556.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3617..3790
                     /locus_tag="OO464_003546"
     CDS             3617..3790
                     /locus_tag="OO464_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228557.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3792..4136
                     /locus_tag="OO464_003547"
     CDS             3792..4136
                     /locus_tag="OO464_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6228558.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4140..4475
                     /locus_tag="OO464_003548"
     CDS             4140..4475
                     /locus_tag="OO464_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6228559.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4532..6853)
                     /locus_tag="OO464_003549"
     CDS             complement(4532..6853)
                     /locus_tag="OO464_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6228560.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6970..7179)
                     /locus_tag="OO464_003550"
     CDS             complement(6970..7179)
                     /locus_tag="OO464_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228561.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7930..9453)
                     /locus_tag="OO464_003551"
     CDS             complement(7930..9453)
                     /locus_tag="OO464_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6228562.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2658 a         2202 c         2076 g         2544 t
ORIGIN      
        1 ccgagatatt tccttgttaa ctctggggaa atgccatctg agttcacaca aatatacggg
       61 tcgcaaacaa cgccgcacgt cggagattga agtccgccag cgtcatgtcc agacgccatt
      121 gatgagaaca tcatccccac acctgacaat ccgatataaa taatacgcat catattcata
      181 gcacatcctg ataatccaac cgtacaacac ttatgctcat ttcgtcatca atccgtaatc
      241 aaaaccgctg acctggacga gttacgatga cctgacagaa gtatcagacg tcttctgcgg
      301 tttctatgat agagcaattc tgtggcggat attaagataa cgggtatctg ttctttaatg
      361 atgctgcaaa aaatggaaag ttaagaaagc ctggcgtggt gaaaaaatac acttgcctca
      421 atactggata tttaaacagg tctggagatg ctgtcacgat tagggtgcgg aaaaaggaag
      481 cgtaaataag aagaatcaaa agtacgacgg caatgggttg attgacaagg gtatttttta
      541 agctatgaat cagcgccatt tatcacagaa tagactttta ctctgaataa atgggagggt
      601 gacttgcctc aatataatcc agactataac atgccttata gtcttcggaa catcatcgcg
      661 cgctggcgat gattaatagc taattgagta aggccaggat gtcaaacact ccaatcgagc
      721 ttaaaggcag tagcttcact ttatctgtgg ttcatctgca tgaggcagaa cctaaggtta
      781 tccatcaggc gctgaaagac aaaatcgctc aggcccccac atttttaaaa catgcccccg
      841 ttgtactcaa cgtcagtgca ctggaagacc cggtaaactg gtcagcgatg cataaggcgg
      901 tttcggcaac cggtttgcgg gttattggcg taagtggctg caaagatgcg caacttaaag
      961 ccgaaattga aaaaatgggg ctgcctatcc tgacggaagg aaaagaaaaa gcgccacgtc
     1021 cagctcccgc accgcaggct ccagcgcaaa atacaatgcc ggtcacaaaa acgcgtttaa
     1081 tagatacccc ggtgcgttcc ggtcagcgta tttatgctcc acaatgtgat ctgattgtta
     1141 caagccacgt tagcgctggg gccgaattga ttgccgatgg gaacattcat gtctatggca
     1201 tgatgcgcgg tcgtgcgctg gcaggggcaa gtggtgaccg ggaaacgcaa atattttgta
     1261 cgaacctgat ggcggaattg gtgtccatcg caggtgaata ctggctgagt gatcaaatcc
     1321 cagcagaatt ttatggcaaa gcggcgcgac tgcagttagt cgaaaacgct ttgaccgttc
     1381 aaccgttaaa ttgatccctt tttaacaagg aatttctatg gcacgcatta ttgttgttac
     1441 ttcgggtaaa gggggtgttg gtaagacaac ctccagcgcg gccatcgcca ctggtttggc
     1501 ccagaaggga aagaaaactg tcgtgataga ttttgatatc ggcctgcgta atctcgacct
     1561 gattatgggt tgcgaacgcc gggtcgttta cgatttcgtc aacgtcattc agggcgatgc
     1621 aacgctaaat caggcgttaa ttaaagataa gcgtactgaa aatctctata ttcttccggc
     1681 atcgcaaaca cgcgataaag atgccctcac ccgtgaaggg gtcgccaaag ttcttgatga
     1741 tctgaaagcg atggattttg aatttatcgt ttgtgactcc ccggcaggga ttgaaaccgg
     1801 tgcgttaatg gcactctatt ttgcagacga agccattatt accaccaacc cggaagtctc
     1861 ctcagtacgc gactctgacc gtattttagg cattctggcg tcaaaatcac gccgcgcaga
     1921 aaatggcgaa gagcctatta aagagcacct gctgttaacg cgctataacc caggccgcgt
     1981 aagcagaggt gacatgctga gcatggaaga tgtgctggag atcctgcgca tcaaactcgt
     2041 cggcgtgatc ccagaggatc aatcagtatt gcgcgcctct aaccagggtg aaccggtcat
     2101 tctcgacatt aacgccgatg cgggtaaagc ctacgcagat accgtagaac gtctgttggg
     2161 agaagaacgt cctttccgct tcattgaaga agagaagaaa ggcttcctca aacgcttgtt
     2221 cggaggataa gttatggcat tactcgattt ctttctctcg cggaagaaga acacagccaa
     2281 cattgcaaaa gaacggctgc agattattgt tgctgaacgc cgtcgcagcg atgcagaacc
     2341 gcattatctg ccgcagttgc gtaaagatat tcttgaggtc atttgtaaat acgtacaaat
     2401 tgatcctgag atggtaaccg tacagcttga gcaaaaagat ggcgatattt ctattcttga
     2461 gctgaacgtg accttaccgg aagcagaaga gctgaaataa gcccgctgta aaaacgcatt
     2521 tatcttcaag gcagagtcat ttctgccttg agtttttcat ccctctcatc cacgttgtgg
     2581 taaagcggcg agtattcttg ctgatactcc tcattgctat ttccacctcc ccctttacac
     2641 cttaaggctg ttcccacgca cagaacaaaa tgtttatgcc tggttgagta aataaccatg
     2701 ttattagtgt gtatatactg ggagatgata accccgcaat ttcattattc attgttctcc
     2761 cataggatga ggtttcccgc cgtaactggc gggctttttt tgccccagaa ttttctcttt
     2821 caacatcctg taaacgaaaa ctgcgccgaa gcgcagttta atcgttgatg gtcagaacga
     2881 ccagttcaca ccagccaccg cgttccacgg ggactccaca ccggcaccat ggctataccc
     2941 caccccgaaa tgcccgctta acgtactgct gaatgaggct ttaatacctg cctggtatat
     3001 tccacgtctg cccgacaaat cattgacaaa attaccgtca ctattcactt tcacccgatt
     3061 atcatcgaca aattctttgc gcacagccgc cttcaggcag ggttcaattt ccataccgtt
     3121 ccccagacgc atgttgtaac tcagcgttgc acccagttca cgatatatac tgcgggtatc
     3181 gactgatttc gattccatgc cattggataa atgatagtag cgtctaagtg acctaagctc
     3241 tgctgcaaca tcagcccgca ggcaccagac caggggattc atcctgaaga gacagcgcag
     3301 gtgtattgtg ttcaccgctc accaaagaca tcatgatgaa atgatgatat cccgcataag
     3361 aaagaggcat tttttacacg cagtgcgctg aagtgtagtt ggataaaaaa tcactctttt
     3421 cagataatac tgtcgtacgc ttatctttag acaatgaggc cgtcggaaaa ataaaatgac
     3481 taatgtaatc gttgctgatg aatatcttct gctcacataa aaatcacaca ataatattga
     3541 taaatcagat tatcttacct ttacaacatt gcgttaacag tcaatgacac ttcttgaata
     3601 aaaatattgt caatcaatga gttattcctc atttaagatt attctcattc acgtaaaaaa
     3661 cataattcct atcatcacag ccacgctcat tttgaattat ctgaataata gcgagcgttc
     3721 tttagttaag cagatcttaa ttgaagacga aattattgtg tgtgcaactt acttaatacc
     3781 ggacatctaa gttgaagaaa aaaatacttg ccttcgggct tatctcagca ttgttctgct
     3841 ccacgccagc gatagcggat atgaaccgca ccacgaaagg tgcattgcta ggcgcaggcg
     3901 ttggtttact tactggcaat ggtgttaatg gcgtactgaa aggtgcagct gttggcgctg
     3961 gtgttagtgc agtaacagaa aaaggccgcg acggtaaaaa tgcgcgtaaa ggtgcaaaag
     4021 tgggtgccgc cgttggtgct gtcactggcg ttctgaccgg caatggcctc gaaggggcga
     4081 ttaaaggtgc cgtgattggc ggtacgggtg gtgcaattct gggtaaaatg aaataaggaa
     4141 tgatgatgaa aaaatttgct ttactggcgg gcctgtttgt tttcgcacca atgacctggg
     4201 cgcaggacta caatattaaa aacggtttgc cgtcagaaac ctatattact tgcgcagaag
     4261 ccaacgaaat ggcaaaaact gacagcgcac aggttgcaga aattgttgcg gtaatgggta
     4321 atgccagcgt tgccagccgt gatttaaaaa ttgagcaatc tccggaactg agcgccaaag
     4381 tggtagaaaa actgaatcag gtttgcgcta aagacccgca aatgctgttg atcacggcaa
     4441 ttgatgacac catgcgtgct atcggcaaaa aataatattt catatgtacc gcgtcgatga
     4501 atacgacgcg gttttttagc taacggcggg atcagaactt atatttcagg cccaccatca
     4561 ctgcggtgtc attgtagcca ttatcaccca gctgcacgcc cacattcccc cagacgctgg
     4621 ccgctggatt caggttgcca tttacgccaa gtttaatttc ccccaaatta ctcaccccat
     4681 cttgttcgac tttcacaccg ttcattgaga cagcaaaatc tttactgtta tggatccagt
     4741 tcatttcaac aaaaggttca aagtcgtcac cgtgcggacc gctgtttttc tcctgagtac
     4801 gaataaaggt gcggaagccg agtcgggtct ggatgttgtt atttgcgtca ttctcaatac
     4861 gtgagccatt ggcttccgtg tgcgcattat gatcgacgcc catccagata gcctgtgcct
     4921 gtggctggaa gatccacgta tatttcgccg cttccagacc aaaggattta ttcagggcaa
     4981 aaccgtaacc cgcttccagc gagacggttg cacctttcgc gctataggat tcataggcta
     5041 attcatcccc tttcacggag tttttaaacc agctatattg cgcccaggag tcaatgtatg
     5101 cgcctttttt actgatgtca tcggcaaacc aagtggcata cagccctgcg ctatagcctc
     5161 tgacactccc tttcgaacga taatccgaca cgctggaatg agttaagttg tagtcgcggg
     5221 cataaccagc catcactccc aaacgccaac tgtcactatc ggtaaaacca ccggttaaca
     5281 gatcgccccc taactgcgag acgtagcgat gcgaggtcgt tctcaactgt ccgttgctgt
     5341 cacgccaggc attatgcccg ccaatttgac gtagccaaag tcggcttgaa cgctcctgtt
     5401 cagttacagg atcgatgtag cgcgtttcac ccgcacggtc gttcagacgc ataacaaaaa
     5461 gagtgttagc ggctgccagg ttggcgacgt aacttccagc ttcggggcgc aggacggggg
     5521 taggcgcaac agtaggcgct gaacttcctc cggagtttgt ttcaccgccg ctcgttgcct
     5581 ggaagttagt cagataccag tttttattgg tcgcttcggt attacctcgg gttaatgaat
     5641 attcgtaggc accggcgaaa atcctcgaat ccttaataaa ttccccattt gattccccct
     5701 caacgctgat aatctctatc ccatttaacg tgtaagctcc gctgcctcct gcattggtaa
     5761 ctttaactcg ggttgttcca gaggtatttc cattaataac gactttatcc gtagctgaat
     5821 tatcatcatc caattcagtt ctgagatgca atacgccatt attaccaacg taattttcag
     5881 ttattgttaa tcttgttggt tcgacatccc gtccatctgc ccgggaaata tagaccgtac
     5941 tattatcaac cgtgaggttg ctcaccgttg agtcagcttt aatatcccag gtactattat
     6001 ctgaaagcga cagataggta tgagtgttat catctgttga aatattagct gaacccgtta
     6061 tttttgaatt agttgcatcg acggtcaaat gacctttaat attattgata aaaacatctg
     6121 cagccgatga tacctcacta tccgtaatgg tcacgttact cgtctcatct ttatggtaga
     6181 ggatttcatt tgctgaccgt agtgagctac cagataaaat atctaccgtt gcttcctgac
     6241 catttatgga agactccagc acaattccag acccctgatg cgcgactgca gatgtatttt
     6301 tcagcgtcac cgtgccgcca ttataaattt tagcaatcgc ggcgtcagga cccgttgtat
     6361 aaatttctcc ctggttaaat gaaactgtcc cctggcgggc agcgatggca tgagcattgt
     6421 ccccatgcgt ggtgatatca acatcggtta atgtaacgct ggcattggta taatcaagcg
     6481 tgtcaccagc cttgacgaca gtgccattag cgtacgcggc ataactttct gttccgtggg
     6541 ttgtgatctc cccttttgcc atatccagtt ttgctatggc attattttca ttacttcctg
     6601 tatcggcagt actttcttca tcaagagaag atacacacgc acacgcgctt ccgggagatg
     6661 cacacgcaag aacgccgact gcgtgattac ccgcagtaac aataaatgta tcatctccgt
     6721 cagaaaaaga gggggtaacc gaaccgtcag agttgactct tattatggtg tcagtattca
     6781 ggtttccact cgtttgactt agcaaaccaa ttgagtaatc ccccgttgtg acaatcgtgt
     6841 ttttaccctg caaaataaca gcaccgccaa attcacttac gattgcgcta gctgttaatg
     6901 ctgggcttag aggactattg tcagaaggat ataaaccgcc cgttgttaca atgacgccat
     6961 tgttaatggt taacttacta ccctctcctg caacggatac ccgtaacttc cattacccgt
     7021 tgttataact agcgcattat caatatcttt tacttttcca tcttttattg ccacagcatg
     7081 tttatattca tcgtgctgaa taaactgttc agcatatgca ttacccaccg ccagagcaat
     7141 aagtcccaaa ctaaacccgg ggagtttttt cagtttcata gattaactat ccattcattg
     7201 gattaacaaa cattcctggt caaataacct atgagctcag attaccaaaa cggagggtaa
     7261 agacaaaaac aaaagcacaa aaaatttaaa tagataaaca gcgacaatat ataaatgata
     7321 aataagtcgg cattatcatt tttcacctta ttttcatgac attgatcact ttggggtgac
     7381 tattttgttt aacaaataac tcttcgacat caaaattatt aatagttgcc atggtaatta
     7441 tttcggtgat atataaaaac aactgtgata acaccaaggt tgacaactat ttgcgcaata
     7501 attcctttcc ttcaatgatc caggcgttca ctgccagcaa cccattcagc cgcatactga
     7561 cagtgagaaa ctgaaagatt attgctgatc cctgtggcta acgagtcttt ctcttaaatc
     7621 gattaaccag attgatcgcc agccaaacaa gggcattaag caaatttatc gtcacaagca
     7681 cttctcccag cccggtactg ggttttgcaa taaggatgac caggagggtg atcagcataa
     7741 atgcaatgga aaagatgata tagagagtga aataatccag attattacta ttattcatgc
     7801 tactaatggc aatcctgtca aaactttcaa acaattatag caaacaaaat aatgaacaat
     7861 agtaacaaga ggcaacgagc taattagctg tctataatta caccgcacta tggccttact
     7921 gatcaatttt cactcaacca caaccttcac cttcggttta gaaaggataa ttttgaccag
     7981 gtcgatgtat gtaacaggtt tatagaaata ataaccctgc tgaaatgtga tattattttg
     8041 gttcagatag ccaagttgtt ctttcgtttc gacaccttca gcgacgatac tgatcgaaag
     8101 tttacgcgcc agatccaata cacaatccag aattcgggtt gattctgggt ctgcgtttac
     8161 gcggccaacg aaactgtgat caattttgat ataatcaata tgcaggtcat gaagataaga
     8221 aagccctgag tagccagtac cgaaatcatc cagcgcgatg acaaaaccat tttcatgcag
     8281 aatgttcaac cgctgaacca gactttcatc aacattcaat ggctcacgct cggtgacttc
     8341 cagaacaagg tttaaatcgc ggcgggtaaa actgtcacgg aaatttaaac actcgtcgac
     8401 aaacgtcggc gaaataatat gcgaagcgct aaaattaatt ccaatatgaa aaccttccgg
     8461 cagtttactc gcgatagcgt tcatctgtct ggcaacctga ttcatcaggc tttgtgtaag
     8521 cggaacgatt aatccggatt tttcagcaag tggaataaat gccgcgggtg aaatatatcc
     8581 accgtgaggt tgtttccagc gggctaacac ctcaactccc cgcaatgtcc cttcccgacc
     8641 atttaccaca ggttggtaaa aagggacgat ctccccatta tttattgccc gtcgcaggat
     8701 ctcttcaggc gttgcgcttt tattgaaata tctatcaagc aggaaggcag cggcgctggc
     8761 gattaacaga ataaatatta aaatcccaaa gcctttatcg ataagtctgt agaagctaaa
     8821 gagtggcggc gggttaaaga taatagtgaa atgatatttt gtggagtgaa ccttctcggt
     8881 atagggataa tgtccattaa gcgtcatcac atcagccgtt ggtccaataa tgtcatccgc
     8941 cacgcgtaat acatacctta cccctttcaa gggtacatta agcgccccgc gaatatgttg
     9001 atcacttatc gtaaccaaaa tgcgcgtatc tgcaaattgg ctctgataga gcaggatagg
     9061 taatctatta acggtgtcga ttgccggagc caacagtaaa ttactgtccg ggacaacagg
     9121 aatacgagac agcaggaccc gattcccagg cagggatgta caccagacta ttccctgttt
     9181 gagaatgatg attgtgcgca ggtgaggttt cagagctgct tcagtgccaa gctgatattg
     9241 ctcctcgagg tcgcattcct tcccggcaat gttaaccgct gtttgagtcg cctgtgacgc
     9301 ttcttcaagt atatgattga tattgttggc ggcatatctt gctcccgcca ggtactctgc
     9361 ttttgcggaa taccagagct ggatgttcaa aatagcgaca ccggtaataa acaagcatat
     9421 cgcaacgagg atgggtatga gtgtattgcg catttgcttg tcccggccaa agggtttaat
//
LOCUS       DAJTUI010000130         9442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTUI010000130 DAJTUI010000000
VERSION     DAJTUI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.130"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            39..1154
                     /locus_tag="OO464_003552"
     CDS             39..1154
                     /locus_tag="OO464_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228563.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            1833..2051
                     /locus_tag="OO464_003553"
     CDS             1833..2051
                     /locus_tag="OO464_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR6228564.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2269..2511
                     /locus_tag="OO464_003554"
     CDS             2269..2511
                     /locus_tag="OO464_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228565.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="OO464_003555"
     CDS             complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="OO464_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6228566.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="OO464_003556"
     CDS             complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="OO464_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6228567.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="OO464_003557"
     CDS             complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="OO464_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6228568.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="OO464_003558"
     CDS             complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="OO464_003558"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7777..7779),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6228569.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8558..9391
                     /gene="fdhD"
                     /locus_tag="OO464_003559"
     CDS             8558..9391
                     /gene="fdhD"
                     /locus_tag="OO464_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6228570.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2311 a         2315 c         2447 g         2369 t
ORIGIN      
        1 ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa
       61 cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga
      121 tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg
      181 tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct tcatcaattg
      241 tgttatgtgc tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc gggataatag
      301 gaaatgtgcc aaaaaataat aaaccaaaaa caattatttt tgatttgcgt tttcttagtg
      361 caattaagca gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag cataccgcaa
      421 tgatgaggga ggcatgtaag gaattaccgc agtcagtgga atatgttcct ttagcctgtg
      481 aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc atcatggcga
      541 caggtgtggg cttggcatta atgtatgcaa catcatcagg accgtattat gcgcaaagtc
      601 tttctggttc gccggatatc gagatgataa aaaatcgcat ggctgaattg tttaaccagt
      661 cggatatttc tgtgcgcaac aaatatagcc cggcttttga taaatggaat gaactggtag
      721 ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attttagcga
      781 cggcgataaa tgaaactctt gagggggata cgaatgttgc cgttgtgatg gggtgtaaat
      841 ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactccttg
      901 aaaaacgcct cgccgatagt ggagaaatag aaaatctttt tgatcaacag ggatatttta
      961 gttgcgattc attaacaccc gaagaattaa tgatgttaaa agaccttttt gatattcgag
     1021 ttcttcatgt ctctaacaaa ttcaatgttg gattgcaagg taatatcaac actgatgttt
     1081 tacaagattc gttttttaaa aatgtcgatt tcattcatga atataactcg tataccgtag
     1141 ggatgggagt ctgacgagta aaactcatta ggattatcag aattcggaca atgcattgca
     1201 aaaatgacat acaaagagag atgtgtgcag gtacttagcc gcgaggggca tggcgaagcc
     1261 atacagaaag taccagtatt cagaactcca gtgaaagcgg catagtggcc gcctgctcag
     1321 gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc gattaaaaaa
     1381 actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg
     1441 aaggaattcc ttgtttcccg aactgttacc aacaaaatta taacgatgag caagtaaaga
     1501 ccggttggtg gcagtactga ccggtctgtg atgaaagatt aatttcacct taaatcctgg
     1561 tgctgttagg atgctgacgt ttttcggcta caccattcag attatgtaaa taaatatccc
     1621 tgaatataat atattcatag cacccataac tcatcctggg cgataactaa accatttttg
     1681 agtaataaat gaaacaactg caacgctctt attatttctg gaaaacgcct cgcaaaaaca
     1741 aaatgacatg caaaaatccg cattttttta aatgcttttc cggcgtaatt ttcgcacata
     1801 cattaatgag taatatatgt aaggtggtgg taatggcgat gaatacggtt tttcttcatt
     1861 tatcagaaga ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag gtttcacgat
     1921 ctgcaatttt acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta
     1981 aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc
     2041 ataaggaatg aaaaaaattt ttcattcttg aatataaaaa acagatgccc ttattctggt
     2101 attaatacaa ggctgtttta cttgaactta taataactgc aactgttaca tcgtatctgg
     2161 aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa gattcggcaa
     2221 gggtactttt ctctccatag attcaaaaat gatattggcg agatatttat gaactctctg
     2281 gcaggtatag atatgggcag aattttactc gatttatcga atgaggtgat taagcaactt
     2341 gatgatctcg aggtgcagcg taatcttcct cgtgcggacc tattaaggga agcggtagat
     2401 caatacctga taaatcaatc gcaaacagca agaaccagtg ttcctggcat ctggcaaggg
     2461 tgtgaggaag atggtgtcga atatcagcgt aagctgcgcg aggaatggta atgggaaaaa
     2521 atgatgtcaa tcagattgct gacaacgtgc gcgttgttca tgccggatgc ggcgtgaacg
     2581 ccttatccgg cctacaaagt catgcaaatt caatatattg aagtttattg gtaggcctga
     2641 taagcgcagc gcatcaggca atcatacgct tgtcatcagt gacataccca taaccttatc
     2701 gatgtgttgg agggaagaac gaggcaaaca gacaattgcg gcatgatctt gaatgggtaa
     2761 cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg cgacctgcgc
     2821 cacccggaaa acgtcagaga ttactctcct tcacccggaa acaggaacgg gttaatggaa
     2881 ctacgggcgt agccttcttg ctccattcgc gcgtccagta ccagagaggc gaggtcatct
     2941 gccacggctt caactttcgg ttctttttcc tgataaagaa tcttcaggta agtgccgcag
     3001 tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt
     3061 ttgccgctct gctcgcagtt gctgcatttt acgcgcacta cgtgccattc ggtttcacac
     3121 aggttgcagt gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc
     3181 ggcatagagc cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg
     3241 ccggggatca gattggccat ctgcgcccag tagagcgaca gcgcagccca gataaacggc
     3301 gctttatcgc tgctgacgga cgagaaatca gaggcaaaca gcgcgctggc catatcttcc
     3361 agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt
     3421 tcaggtttca gctcagcaat tagcgccatc agcagctttt gccagtgctt atcacgcggc
     3481 agaacgtgaa tatccagcgg gggcttgccc tgtgcgctgg cttctttaat gcgcgcggtc
     3541 agatccatct ccagcggatg gtcgtacagc accactttct gggcgtgggc gataagcgca
     3601 gcaaagcgca ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg
     3661 cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta
     3721 cgtttctcgc tcgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct
     3781 tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc
     3841 tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga
     3901 tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga
     3961 atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata atcacgccgc
     4021 tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt
     4081 tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc
     4141 aaaagatatc atcccgattg attaggtttt gatgccagta acggaaaaac atgatgatga
     4201 acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc tgcggtgtgc
     4261 ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg
     4321 ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca
     4381 cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc
     4441 ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg aagatactgg ccgcaaaggt
     4501 agccgcaaag ccaacagccg cgagcggttt ccagatgcct ttccagaatt tcacggtttc
     4561 gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag
     4621 cacgtacatg acgtgtgtac caccgacgcc tgccggatcg tacagacccg cattgtcgta
     4681 accgcgggtt ttcagctcag caacgcgctc gctcgccagc gttttcatcg actctttcgt
     4741 gccgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt gcccaaccac
     4801 cacgcggtca acgcacagcg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg
     4861 aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct cggactggaa
     4921 gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg ctttcaggca
     4981 gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt
     5041 ctgctccact tccgagaagc gcattaccgt ccacgatttg gcgcttaaat cattggggtt
     5101 gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc
     5161 cacctgacag gctttacagc cgatacaggt ggtaacgtcg atgagtttcg ccacttcttc
     5221 ctggaagtcc cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat
     5281 atcttgagat tgataagcca tatgtcgtct ccgttacacc ttttccacat tcacaaggaa
     5341 ggacttaaac tccggcgtct gcgtgttcgc atcaccaacg aatggcgtta aagtattggc
     5401 aataaagcct tttttagcta cgccttcata gccccagtga atcggaatac cgatggtatc
     5461 gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg ctttggcttt
     5521 gatatagcca cggttggagg agactttcac ggtatcgccc tgggcaatgc caagtttatt
     5581 cgccagcgac tccccgattt ccacaaactg ctctggctgc aaaatcgcgt tcaacagcgc
     5641 gtgtttggtc cagtagtgga agtgctcggt cagacgatag gtggttccga catacgggaa
     5701 cttatcggct ttacccaatg cttcggcgtc gtctttaaag atacgtgcag ctgggttcga
     5761 gataacgttt ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc
     5821 cgggaacgga ccttccgcca tcttatcgag ggcaaacaga cgccccatgc cttcctgctg
     5881 catgataaac ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg gaatatccca
     5941 gccggtccac ttagtgccgt cccatttcag caactgacgc ttcggatccc acgggttacc
     6001 ctgcggatct gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc atgcccatgc
     6061 ccagcccagc gtgttaccga ggccagacgg atcggcgtta tcacgacgcg ccatctggtt
     6121 gccttccggc gtccagctac cggcgaaaat ccagcagcca caggaggttg taccgtcatc
     6181 gcgcaattgg gcgaacgagc taagttgttg gcctttcttg acgataaccg caccggttgc
     6241 cggatcggta atatcggcca gcgccttacc gttgctctcc atcgctactt cttccgactt
     6301 cggctcatgc ggaatggcgt agttccaggt catgttcagc acctgatccg ggttcgcgcc
     6361 accctgttcg gcatacatct tgcgcaagcg caggaagata ccggagagga tctcaccgtc
     6421 agtcagcgca atccccgggg cgtccgcacc tttccagtgc cactgcaacc agcgacctga
     6481 gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga acacttcggt
     6541 ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga agttagaggt
     6601 ttcagtgttc agtgggtcga tggtgacgag gaacttcagt ttcgacagac aaccgatcac
     6661 tttgtttttg ttcgggaatg aggcaacagg gttaaagccc tggcagatat agccattgac
     6721 cttgccctct ttcatcatct cgaagtattg caggacgtcg tagcctttat cccacttcgg
     6781 caaccagtca aagccccagc tattttccgc cgtcgcttta tcaccaaaga aggccttcat
     6841 catagagacg aagaatttcg ggtagttgcc ccagtagttt acctggcctt ccagcagcgg
     6901 ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc ttggcagcgt
     6961 catgtaacct ggcagactct gcgacagtag ccccaggtcc gtcagcccct gaatattgga
     7021 gtgaccgcgc agggcgttaa cgccgccgcc ggccatcccc atattgccga gcagcagctg
     7081 gatcatcgcc atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc agccgagggc
     7141 atacaggaac gaggcagttt tatcgtgagc actggtttct gcgatgtatt cgcagacttt
     7201 caggaacgcg tcttttggcg taccacagat gttttcaacc acatctggcg tgtagcggga
     7261 aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg tatcgcgttt
     7321 ggcgaagccg ttttcgtcca gttcataagt ccaggaggat ttatcgtact tgcgtttttc
     7381 cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca cgatcaggct
     7441 ggcgttggta taggcttcgg tgtattcgtg gttgaatttt tcattgttca gcaggtacag
     7501 caatacgcct gacaggaaag caatgtcagt accggaacga ataggggcat agtagtcagc
     7561 taccgccgcc gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc cgttgtgaat
     7621 tttggcttcc atcgcccagc ggaacccgac cgggtgagct tcagcggcgt taccgcccat
     7681 caccacgacg aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc
     7741 aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac
     7801 cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa tagccggttt cgttactcga
     7861 cgcggaagca cacagcatcc cggtggagag ccagcggtta acagtcacgc cttcggcgtt
     7921 ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc
     7981 ctcttcccaa ctgatttgct gccatttatc agaacctggc gcacggtatt ccggaaactt
     8041 cagacggctt tcggagtgga tgaaatccac caggccagcg cctttcggac aaagtgcacc
     8101 gcggttgacc gggtgatccg gatcgccttc gatatggaag atagatgctt tggcgttttt
     8161 tgctccgtca ccgaggctgt acatcaacag cccacagcct acggaacaat aggtgcaggt
     8221 attacgggtt tcgcgggtgc gcagcagttt atactgccgg gtttccgcga gtgctacgct
     8281 gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata ccgccagcgc agatcttaaa
     8341 gaactgcctt ctgctgacct gcatggattg ctccttgttt cgacattgtc acgtcccatt
     8401 tacattcgct tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg aaggggccgc
     8461 aaaggtccag aattggctca atttccctcc atctaggaag ggtttgtaac agaataccat
     8521 aatgttggtg tgtatgttct tatctggtta agagaaagtg aaaaaaacac agcgaaaaga
     8581 aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc ggcgtgatga
     8641 tttacaacac ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc tggtctacaa
     8701 cggcatttcg catgtggtga tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg
     8761 tttttcgctt tccgagggga taatagaaag tccgcgcgat atcttcggca tggatgtcgt
     8821 tccttcctgt aatggtcttg aagtacaaat tgagctttcc agccgccgct ttatggggtt
     8881 gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg gcgtggagca
     8941 acttaatgac atcggaaaac cggtgcagcc gctaccgttc acccagacgt ttgatctcaa
     9001 caaactggat gatgcattac gtcatctcaa cgattttcag ccagtggggc gactgactgg
     9061 ttgtactcac gccgctgcct ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga
     9121 cgtgggtcgc catgtggcgc tggacaaact gttaggccgc cggtcacaag aaggggaaag
     9181 ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg tgcaaaagtc
     9241 ggcgatgtgc ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacgc ttgctgtaga
     9301 agtggctgag cgctgtaatc tgacactggt aggtttttgt aaaccgggtc gggcaacggt
     9361 ttatacccat ccgcagcgtt tgagcaatta attaaaataa gtcctgcgat atatattaaa
     9421 ttgcaggact tattcatttc gt
//
LOCUS       DAJTUI010000131         9291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTUI010000131 DAJTUI010000000
VERSION     DAJTUI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.131"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="OO464_003560"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="OO464_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR6228571.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="OO464_003561"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="OO464_003561"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6228572.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="OO464_003562"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="OO464_003562"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6228573.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="OO464_003563"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="OO464_003563"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6228574.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="OO464_003564"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="OO464_003564"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6228575.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="OO464_003565"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="OO464_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6228576.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6164..6799)
                     /gene="nth"
                     /locus_tag="OO464_003566"
     CDS             complement(6164..6799)
                     /gene="nth"
                     /locus_tag="OO464_003566"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6228577.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="OO464_003567"
     CDS             complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="OO464_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6228578.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="OO464_003568"
     CDS             complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="OO464_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6228579.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="OO464_003569"
     CDS             complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="OO464_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6228580.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2384 a         2326 c         2357 g         2224 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat
     5581 taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga
     5641 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     5701 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     5761 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg
     5821 cggcgatatg ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     5881 agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa
     5941 cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata
     6001 gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta
     6061 cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat
     6121 aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct
     6181 ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc
     6241 aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa
     6301 ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac
     6361 gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt
     6421 ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat
     6481 cttccggaac ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt
     6541 ctgctttgct gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta
     6601 gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa
     6661 cactgatatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac
     6721 tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca
     6781 gacgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc
     6841 agctgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat
     6901 cagtcccaag ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt
     6961 gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc
     7021 aaacaacgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt
     7081 tgcgcccgta ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc
     7141 gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc
     7201 taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac
     7261 cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag
     7321 cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag
     7381 agcgttagtg gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag
     7441 tgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta
     7501 ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc
     7561 gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa
     7621 atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc
     7681 cgcaaaatgg gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt
     7741 ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag
     7801 cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg
     7861 tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt
     7921 taccagatag caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc
     7981 aaataacgtc ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt
     8041 gatggccgca gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt
     8101 tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta
     8161 gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg
     8221 atagccgccg aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag
     8281 ccgaccacga ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag
     8341 catagtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa
     8401 ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg
     8461 aatcgctttc tgccatagca accacacgcc accagccagc caggcgagat ttacccattg
     8521 ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga
     8581 atgacctgcg cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc
     8641 aatacctaag cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac
     8701 ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct
     8761 ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa
     8821 cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc
     8881 cagtacgacc atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt
     8941 cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac
     9001 gagagcttcg gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca
     9061 accaaaaaac cgcagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat
     9121 aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat
     9181 ttagtcctcg tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc
     9241 cgcggcaact gcggctttgc gcggatcgac ctgttcttct ggttccgcat t
//
LOCUS       DAJTUI010000132         9290 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTUI010000132 DAJTUI010000000
VERSION     DAJTUI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.132"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            17..868
                     /gene="hchA"
                     /locus_tag="OO464_003570"
     CDS             17..868
                     /gene="hchA"
                     /locus_tag="OO464_003570"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6228581.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(976..2334)
                     /gene="hprS"
                     /locus_tag="OO464_003571"
     CDS             complement(976..2334)
                     /gene="hprS"
                     /locus_tag="OO464_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6228582.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2334..3005)
                     /gene="hprR"
                     /locus_tag="OO464_003572"
     CDS             complement(2334..3005)
                     /gene="hprR"
                     /locus_tag="OO464_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6228583.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3138..3551
                     /gene="hiuH"
                     /locus_tag="OO464_003573"
     CDS             3138..3551
                     /gene="hiuH"
                     /locus_tag="OO464_003573"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6228584.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3660..4664
                     /gene="msrP"
                     /locus_tag="OO464_003574"
                     /pseudo
     CDS             3660..4664
                     /gene="msrP"
                     /locus_tag="OO464_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4665..5300
                     /gene="msrQ"
                     /locus_tag="OO464_003575"
     CDS             4665..5300
                     /gene="msrQ"
                     /locus_tag="OO464_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR6228585.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5558..6208
                     /gene="zinT"
                     /locus_tag="OO464_003576"
     CDS             5558..6208
                     /gene="zinT"
                     /locus_tag="OO464_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6228586.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(7286..7555)
                     /locus_tag="OO464_003577"
     CDS             complement(7286..7555)
                     /locus_tag="OO464_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6228587.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            7612..8280
                     /locus_tag="OO464_003578"
     CDS             7612..8280
                     /locus_tag="OO464_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6228588.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            8596..>9290
                     /locus_tag="OO464_003579"
     CDS             8596..>9290
                     /locus_tag="OO464_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6228589.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT         2667 a         2092 c         1897 g         2634 t
ORIGIN      
        1 caataaggaa tacactatga ctgttcaaac aagtaaaaac ccgcaggtcg atattgctga
       61 agataatgcg ttcttccctt cagaatattc gcttagccaa tatacgagtc ccgtctctga
      121 tcttgatggc gtggactatc caaaaccgta tcgcggtaaa cataaaattc tggtgatcgc
      181 cgcggacgaa cgttatttgc cgaccgataa cggaaaactg ttctcgaccg gtaaccatcc
      241 gattgaaacg ttgctgccgt tgtatcatct ccatgctgca ggtttcgaat tcgaagtggc
      301 gaccatttcc ggtctgatga ccaagtttga atactgggct atgccgcaca aagatgaaaa
      361 agtaatgcca ttctttgaac agcataaatc gttattccgt aatccgaaga aactcgcgga
      421 tgttgtcgcc agcctcaacg ctgatagcga atatgcagca atctttgttc ctggtgggca
      481 tggcgcactt attggtttac cagaaagcca ggacgtggct gccgctttac agtgggcaat
      541 caaaaatgac cgttttgtta tctccctttg ccacggtccg gcggcttttc tggcacttcg
      601 ccacagcgat aacccactga atggttattc tatttgcgca ttcccagacg ccgcagacaa
      661 acaaacgcca gatattggct atatgccggg tcatctcacc tggtacttcg gcgaagagtt
      721 gaagaaaatg ggcatgaaca tcattaatga cgacatcacc gggcgagtac ataaggaccg
      781 taaacgtctc accggcgaca gtccttttgc agcgaatgcg ttgggtaaac tggcggcgca
      841 ggaaatgctg gcagcttacg cgagttaatc cctgttacat tgtgatgtaa gcgcataatc
      901 aatcgcagcc acgttaatac tcacgacgtg gctgcgtttt ttcacggtta acttatggcg
      961 tactgaagcc cgatgttaat ttctttgcgg taacgtaatt cggaacacgt tatgcttact
     1021 gagatagtga tacgtagcac ttcctccatg taattcggca atcgctttga ctaaagaaag
     1081 gccaagtccc tgacctacgg aatgacgcga attatctccc cgccaaaatc tacggaagag
     1141 tttttcaggc tcattaattt tcgttccagg actggcgata tcaatattaa gagagccgtt
     1201 ggcatcaaga aaactggtta tatgaatacg cgatttttct ggtgaatatc gaatggcgtt
     1261 aacaataaga ttcgataaca ttcgttgcag taaaatttta tccgcaaaga tttgttgatt
     1321 gcacttgacc ttaaagcaaa tctctttctc gtctgaaaga tattcaagat agtccaaaag
     1381 attttcaact tccttattga gagaaagcga atccagtttc accaaaacat tgtttttgtc
     1441 tgccctggca agaaacagta tattctctgt taaccgcgaa atattttcca gctcttcaat
     1501 gtttccggca attgtttttt gatattcagc gatacttctg atttgactga gcgtaacctg
     1561 attctgaccc agtaatgcat taattggcgt tctaagttca tgagcgagat cgtcagcaaa
     1621 ttgactaagg cgctcaaaat ctttgactaa agcatgatgc attttattca acgcctgccc
     1681 aagaggtttt agttctctcg gtaatgcgtt aacctcaacc ggcactcggc tatcgttata
     1741 attcagcgct tccgttacgc cactcaactt tttgatctct cgcaaccccg tcctgattaa
     1801 caacggactt aatactgagc acagtataat ggcgacaatg caaattataa tgctattaat
     1861 tttatactgt tcaagcatat tatgtctggc tgaagccaat ttagccacag taaccgttaa
     1921 tgatggcgaa acttcatcaa tgttaattcg taaagcatct atctctgtat cattaataat
     1981 gcttctgtaa atgcctgctg cgctgattgt ctcactagca ggtatgttat ttaacatgtc
     2041 atcactgaca tttgtccggt taacaatttt attgatgcca tcactatgaa tgatcaaaat
     2101 atcctgacta acatccatca tccggttaaa gtacacaggt aacgtatctg gatttacccc
     2161 atcaattaac aactgcttga tctgcgctgt ccggttaatg agtgttgtat catcgcgcca
     2221 tttcaactca cttgccaggc cattatagag agtccataca attccagcgc cagcaacaga
     2281 ctgcagaaat ataaaaagca aggttaaacg gacggttata gataatcttt tcattatttt
     2341 tttaccgcta cgaatgaata gcccatcccc cggattgtgg cgattagctt ttcaggaaaa
     2401 ggatcatcaa cttttgcgcg gagcctgcga atggcgacat ccaccgtatt ggtatcacta
     2461 tcaaagttga ttccccaaat ttcactcgca ataaccgttc tgggtataat ctcgccagct
     2521 ctggaggcca gtagccaaag taactgaaac tccttgcgcg tcagtgtaat actgatattg
     2581 tccctgctga cactttgact gacagagtcc atttttaagc cactgatttc taatgttgaa
     2641 ttcagagcgt gatgttgcct taattgtgcc cgaacccttg ctagcaactc agaaaatgaa
     2701 aaaggtttta ccagataatc atttgcccca ctgtccaacc ctctgactct gtcatcgaca
     2761 gaatcccttg cagtaaggca aataacaggg gtttgctttg ccgttcttaa cgtttgtaag
     2821 atctgccagc catccatacc cggaagcata atatccagaa tgatcaatgc ataatcatcc
     2881 ttcagcgcaa gataaagccc atctctgcca tcagaaacgg catcaatgac ataacccgct
     2941 tcggaaagcc cctgcgttac ccattcctgg gtcctttgat tatcttcaat aagtagaatc
     3001 ttcatgaaaa taatatgcca taaatcatat gttatagaaa caacctggtt cattacaaaa
     3061 ttgtaatgct gctgtaaggt taccctggac gttttttcgc tatcctcaaa actcattcac
     3121 atgacaagga tataaacatg ttaaagcgtt atttagtact ctcggtagca acggcagcct
     3181 tttcattacc ttctttggtt tatgccgcac aacaaaacat tcttagcgtg cacattttga
     3241 accagcaaac aggaaaaccc gctgccgacg tgacagtcac tcttgaaaag aaagcggata
     3301 acggctggtt acaacttaat accgccaaaa cagataagga tggacgaatt aaggcactgt
     3361 ggcccgagca aactgcgact actggcgatt accgtgtcgt atttaaaacc ggggactatt
     3421 tcaagaaaca aaatcttgaa agtttcttcc ctgagattcc cgttgaattt catattaata
     3481 aagtgaacga gcattatcat gtgcctttgt tactcagcca atatgggtat tcaacctatc
     3541 gtggcagtta atgcgtaaat atgtaaaagc tgatctctgc tactgttagg gaggtgactc
     3601 cctgacagat taacagtaaa cagctcttgt tggttaacga caaaaaagtg tgatggctta
     3661 tgaaaaagaa gcaattttta aaagaatcag atgttacggc cgagtcggta ttctttatga
     3721 agcgtcggca ggtgttaaaa gcactgggca tcagcgcagc tgcattttct ttgcctcacg
     3781 ctgcgcatgc cgatctgctt agctggttta aagggaacga tcgtccgccc gcccccgccg
     3841 gaaaaccgct ggagttcagc aagcctgccg cctagcaaaa taacctgcca ctgacgccag
     3901 cagataaagt ctccggttat aacaacttct atgaattcgg gctggataaa gacgatcccg
     3961 cggctaatgc tggtagcctg aaaaccgatc catggacact gaaaatcagc ggcgaagtgg
     4021 caaaaccatt gaccctcgat catgatgatt taacccgtcg cttcccgctg gaagagcgta
     4081 tttatcgtat gcgctgcgtg gaagcgtggt cgatggtggt gccgtggatt ggttttccgc
     4141 tacacaaatt gctggcgctt gccgaaccca ccagcaatgc gaagtatgtc gctttcgaaa
     4201 cgatttatgc gccggaacag atgcctggcc agcaggaccg ctttatcggc ggcgggctga
     4261 aatatcctta tgtcgaagga ttgcgtctcg acgaagcaat gcatccgctc acactgatga
     4321 ccgtaggtgt ttatggcaag gcgttaccac cgcaaaatgg cgcgccggtg cgactgattg
     4381 tgccgtggaa atatggcttt aaagggatta aatcgatcgt cagtattaag ctgacccgcg
     4441 agcgtccgcc aaccacctgg aatctggcag cgcctgacga atacggtttt tacgccaacg
     4501 ttaatccgca tgttgatcac ccgcgctggt cacaggctac cgaacgattt attggttcag
     4561 gcggcattct cgatgttcag cgccagccaa cactactgtt taatggttac gccgaccagg
     4621 tggcatcgct gtatcgtggc ctggatttgc gggagaattt ctaaatgcgt ctgacagcaa
     4681 aacaagtgat atggctgaaa gtttgcctgc atcttgccgg attgttgccg tttctctggc
     4741 tggtctgggc gatcaatcac ggtggactgg gtgccgatcc ggtgaaagat attcagcatt
     4801 ttaccggtcg cactgcactg aaatttttgc ttgcggcctt gttaatcaca cctctggcac
     4861 gctacgcaaa acagccgtta ttgatacgca ctcgccgcct gttaggctta tggtgctttg
     4921 cctgggcgac actgcattta accagttacg cattgctgga gttgggcgtg aataatctgg
     4981 cgttattagg taaggagtta attacccggc cttatttaac gttaggaatt atcagctggg
     5041 ttattttgct tgctttagcg ttcacttcaa cccagtcgat gcagcgaaaa ctcggcaaac
     5101 actggcaaca gttgcataac ttcgtctatc ttgtcgcgat cctggcccca atacattatc
     5161 tgtggtcagt gaagattatt tcaccgcagc cactcatcta cgccgggctg gctgtactgc
     5221 ttttagcctt acggtataag aagttgcttt cccttttcaa ccggttacgc aagcaggccc
     5281 acaacaaatt atctctataa cttcatattg ccgacaaact tcgcgttatc ccggccggat
     5341 gcggcgcgag cgccctatcc ggcccacaca gatcgcatca agttatgttt cgtttgtcat
     5401 caacctttca tcaccagaat aatttatttt atttcaataa gatagttcaa tacacctgag
     5461 aataccatgc tctcatttcc taatgagttg ttgcattttt tcatatgtta caatataaca
     5521 ttacacataa tgtacattaa ctctggagga aattgttttg gcgattcgtc ttcataaact
     5581 ggctgttgct ttaggtgtct ttattgttag cgctcctgcc ttttcgcatg gtcatcattc
     5641 tcatggcaaa cccttaacag aggtcgaaca aaaagctgct aatggtgttt ttgatgatac
     5701 caatgtacaa aaccgaacgc tcagtgactg ggatggagtc tggcaatccg tttacccttt
     5761 actgcaaagt ggcaaacttg atcccgtctt tcagaagaaa gcggatgcag ataaaaccaa
     5821 aacatttgct gaaattaaag attattatcg caaaggttat ccgacagata tcgaaatgat
     5881 tggcattgag gacggcattg tcgaattcca tagaaataat gaaacaacat cctgtaaata
     5941 tgattacgat ggatacaaaa tactcaccta taaatcaggc aagaaaggcg ttcgttactt
     6001 attcgaatgt aaagatcctg aaagcaaagc ccctaaatat atacaattta gcgatcatat
     6061 aattgcacca cgaaaatcat ctcattttca catttttatg ggtaatgatt cccagcaatc
     6121 tttattgaat gaaatggaaa actggccaac gtattatccc tatcagttga gtagcgaaga
     6181 agtggtcgag gagatgatgt ctcattgatt tttttgcgct gacttcagtt atttaacaga
     6241 acagtgcctg gtagtaacaa caataattta ccaagacaaa taagtaagac aaactcagac
     6301 acgctcatac ttgagtcgtg tcttttttat tatgtctgta gaaaaaaact gagaaaaagg
     6361 ctaacaataa atacacaact cagccataca gactggcagc gcaaatgttt ccaggagcaa
     6421 gtcatctgtc atggtaaagc taaccagaaa tataggttaa ttttggtgtt taaacctttt
     6481 ttacttgtta gtatgataca aaggctttca aaaaagctgg gaaattcaac aactcttgga
     6541 aattatggac tttgtcccgc ggctttaatc tgacggccgc gttccttttt tggctcaatt
     6601 attgacccct tctctacgca acttcagttc ccgccagcaa ctttgcggca gtttcatagg
     6661 atcaatgtct aaaagaataa tggtgaccga taagaaaacg actgaataac tgtagatttt
     6721 cgctcgaaac cttcctgtca gctccatagc gaatcaagcg ctgaatgtca cagtatcgaa
     6781 cagaaaacag tgacgatcta acccttcaag aatattctac gattgttctg tttaggaaaa
     6841 gcaaggcggg aagtcgggag ataagtcatt gataaaatgg cggagagagg gggatttgaa
     6901 cccccggtag agttgcccct actccggttt tcgagaccga tgatacagag tcgaaaaatc
     6961 aaagagttaa aaatttttct tggaaaaaat gtcttaaaat cgtacacgaa aaatcaatga
     7021 attacataac attaaagaag aatattccaa gccttcttga gtccaccacc tgttttcagg
     7081 tgtcattcag acgtgatttg ccccagattg ggaaagcacg cttttttctc agcttattcc
     7141 gttttgcctg tgtgttgaat aattccactg cagttatata aaatcggtaa cggctggaaa
     7201 tcattcaata ctcgcactat cgaaagttta ccagccaacc gcagcacgtc ctgcatacgt
     7261 cgtgtctgcg gtttttcttt ttcgcttaca ttgtgtctgg ttcttccggc cactcaatat
     7321 caggtgcagt tgatgtatca acacggttca gcaacacccg atacttcttc caggcttcca
     7381 gcaacgagtt ttcttcctcc gttgcgatct ccagatctac agcatcctga agtggcgcaa
     7441 tatgctcact gaatccctgg atgtagaact gtgtggtgac ggtcttccag ccattcggct
     7501 cctgctgtat cgaagcatac caggctattt caatatcgct atgctgcggc agcatttaac
     7561 cccttgtaat tcatcgccat aattgattta attcacaaat aaaactataa tatggtgaaa
     7621 ttaatgaaaa aaaacacaga tgatggggct aaaatttaca caccacttac cctaaagctt
     7681 tatgactggt gggttttggg agtatcaaat cggcttgcat ggggatgtcc tacaaaggaa
     7741 caccttcttc cacactttct ggaacattta ggtaacaacc atctggatat tggtgttgga
     7801 actgggtttt accttactca cgtacctgag agtagtctga tatctttaat ggatttgaac
     7861 gaagctagcc tgaacgcggc atctacaagg gctggggaat caaaaattaa acataaaatt
     7921 agccatgatg tttttgaacc ttatcctgcg gcgttacatg gtcaatttga ttccatttcc
     7981 atgttttacc ttcttcactg cctgcctgga aatatatcta caaaaagctg tgtaatacgc
     8041 aatgcggcgc aggccttaac tgatgatgga actctatacg gagccacaat tcttggcgat
     8101 ggagttgtgc acaatagctt cggtcaaaaa ctgatgcgca tttacaatca gaaaggcatc
     8161 ttttcaaaca caaaagattc cgaagaaggc ttaacacata tactctcaga gcatttcgag
     8221 aatgttaaaa ccaaggttca aggtactgta gtaatgtttt ccgcttcagg gaaaaaatag
     8281 catccaaccg cagcacgttc ttgcttaaga cgtgctacgg tttcctttac gatttggggt
     8341 tgcgacttta cctctattga taatgcattc cggcagaacg ttcaaatatg aacgtacgat
     8401 atttaactaa ccgaaaaaca aaatatagca caggcgagac atttaccatc taagaaaata
     8461 gtatcgtttt tttacagtta aatcaacatc acttccttaa aatgaaaaca ataaatttca
     8521 atcctgaaac attttatttc agcttattat tccccagtat cacattttat caggatatcc
     8581 ttctgcgagg ttattatgct tcctgtaaat aatccccccc tatccactgg aaacgtctct
     8641 ttttacagaa ctacatcaat cgacaatgtt cacaataatt atctctccga atgggttgaa
     8701 tggactaaaa acagcatttc cggagaaaac agggaaactg cttttacccg gctccaatta
     8761 tgtctggaga acagtgaaac atcgttggac ttatcttgtt taggtctcag atctctacca
     8821 cgattgcctg acaatcttga tgaaattaat gtaagcaata accaactatc aatgctcccc
     8881 gagctaccaa gggcattgaa agagctgaat gcaagcagta atcaattatc tgcacttcct
     8941 gaattaccag tgtcgctgga atatataaat gtgagtgata accatttgtt cgcacttcct
     9001 gaattacctg cgtcactaga atatattaat gtaagtgaca atcacctgtc tgtacttccg
     9061 aggttaccaa tgtcattgga attacttgat gcagccagaa atgctttgga agtaatacca
     9121 gattttccag aaagagatga tcatattata agaatattct ggcttaatca gaaccggatc
     9181 acggcaattc cggaaagcat acttggcctc agttctgata gcgttgtcaa tcttagagaa
     9241 aatcaactat ctcccagaat aatgcaaact ttgttacaac aaaccgccca
//
LOCUS       DAJTUI010000133         9243 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTUI010000133 DAJTUI010000000
VERSION     DAJTUI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.133"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO464_003580"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO464_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6228590.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO464_003581"
     CDS             1828..3147
                     /locus_tag="OO464_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6228591.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO464_003582"
     CDS             3445..3486
                     /locus_tag="OO464_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228592.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO464_003583"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO464_003583"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR6228593.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO464_003584"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO464_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO464_003585"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO464_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO464_003586"
     CDS             5815..6711
                     /locus_tag="OO464_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6228594.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="OO464_003587"
     CDS             6708..7682
                     /locus_tag="OO464_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6228595.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO464_003588"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO464_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO464_003589"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO464_003589"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6228596.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2260 a         2257 c         2317 g         2409 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctca
     9241 aga
//
LOCUS       DAJTUI010000134         9235 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTUI010000134 DAJTUI010000000
VERSION     DAJTUI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9235
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.134"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..613)
                     /locus_tag="OO464_003590"
     CDS             complement(<1..613)
                     /locus_tag="OO464_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCR6228597.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRVMLPT"
     gene            complement(680..1405)
                     /gene="fimC"
                     /locus_tag="OO464_003591"
     CDS             complement(680..1405)
                     /gene="fimC"
                     /locus_tag="OO464_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6228598.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1442..1981)
                     /locus_tag="OO464_003592"
     CDS             complement(1442..1981)
                     /locus_tag="OO464_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6228599.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2046..2594)
                     /gene="fimA"
                     /locus_tag="OO464_003593"
     CDS             complement(2046..2594)
                     /gene="fimA"
                     /locus_tag="OO464_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6228600.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3075..3671)
                     /gene="fimE"
                     /locus_tag="OO464_003594"
     CDS             complement(3075..3671)
                     /gene="fimE"
                     /locus_tag="OO464_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR6228601.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(4149..4751)
                     /gene="fimB"
                     /locus_tag="OO464_003595"
                     /pseudo
     CDS             complement(4149..4751)
                     /gene="fimB"
                     /locus_tag="OO464_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            6205..6921
                     /gene="nanC"
                     /locus_tag="OO464_003596"
     CDS             6205..6921
                     /gene="nanC"
                     /locus_tag="OO464_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6228602.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6941..8047
                     /gene="nanM"
                     /locus_tag="OO464_003597"
     CDS             6941..8047
                     /gene="nanM"
                     /locus_tag="OO464_003597"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6228603.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8112..9092
                     /locus_tag="OO464_003598"
     CDS             8112..9092
                     /locus_tag="OO464_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6228604.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2711 a         2010 c         1940 g         2574 t
ORIGIN      
        1 aagttggcag cattacccga ttctgtacac tatttccgct gaaattataa ttgagcaatc
       61 cggcattaat accgggatcc cataactcag gaggaatata accacgcgcg cgattactca
      121 taaatgcctg agggatcgtc aggttcagtc gctgctgacc aacatccaga tgcgcagtag
      181 cgtcctggac cattgtggtt aatggcacac aggcatcatc cgccagcaga ttcataccgg
      241 cgacagaagc cgtattcagc cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa
      301 tcccttgttc actgtcgccc gtattaaatg tgacatcacg cgttgccata taaccattat
      361 tcaaatagat atcgacgcga tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg
      421 ataaatcggc cacagcctgg ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg
      481 cagatgacaa aggtgcctgt gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac
      541 cagccaaacg atgcttacga atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat
      601 tcagatatga cattattcct ccggtttaaa atcacccggc agtcaattct ttttattcag
      661 gcgaaaaatt ccccctgcgt tattccatta cgcccgtcat tttgggggta agtgcgccat
      721 aatcatttat tgttcggtaa gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc
      781 tttcgcccat tggaggcacc aatgcatttt caagaacccg ggttccggca ttcaactctg
      841 ttaccgtcag gtaatagggt gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa
      901 atcttaattt ttctgcggcc tgatcgggtg gcaacgctaa tttagccggg cgatagtaca
      961 gtttaatgcg gctgataatt gcgagctgta gcatattctc agtcaatttt gatttatcca
     1021 ttgacggaat cgctttaacg ttcatccaga ataaactttc ccggtcctgt ggcaattggt
     1081 tatttgttgc atcaagaata cgtaaggtat tctctttttt tcccttcatc gcaaacagag
     1141 gaggcgtcac gataaaacga ccatccttta caccatcggc attttccacc catgattgaa
     1201 ttaaataggt actattttca tcattatttg tcacggcaag ttgcacttgt ttttgccctg
     1261 ccggataaat tacgcgagtc gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca
     1321 tcattgccat gaacatcagg atacctgcca gcaagcagaa tgttatttcc tgcgatttcc
     1381 ttacattgac gtttttatta ctcactgtcc tgttcctgtt atcacattat ctactgaaca
     1441 attactgata ggttaaagag aaccaggcct gggcattggc gatgccgcca gtaacccgac
     1501 gcccggtagc acgatatttg gcgatgaaat gtagcgaagt agagcctgag taaagccgtt
     1561 tccagtttgc tggaggacga ttaatcggta cgaggtttcc ttcatcatca aacaacgcta
     1621 caccaatatt ggaggctatc cctggcccct ctcccacgga gagcacatcc ggatttttac
     1681 catccgcgac accgtgaaac gccacaccca cacgttcact caccaccgtg ctacattccc
     1741 gtaaatgaat aacaaaaggc accggtgcgc tatcttcccc tgccgcatga aaccggttac
     1801 tgctgatttg ccccatattg accgtcattt gtttatcacc ggcttcaatc cggcaagttt
     1861 ccgcaataat gacgccctga aattgcatat ttccgccggg cagcgtggta ttccatttat
     1921 ttccggccag agcaaacatt ggcagcaacg acgctaacag aaataatctc ttccttttca
     1981 tcgttatttt tatcgcacaa gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg
     2041 gtaggttatt gatactgaac cttgaaggtt gcatccgcat tagctgcacc cggggttgcc
     2101 tcgccgattg cataataacg cgcctggaac ggaatggtgt tggtaccgtt attcagggtt
     2161 gtttgtgcac taaatgtcgc accgtccagc gtcagagcat tgcctgttct gtctaggatc
     2221 tgcacgccaa cgtttgttgc actacctgca gcagaactct gcagagccag tacatcagta
     2281 cgcgtagcat caattgccgt acctaagaag gcaacagcgg cttttgtggc aacagtggta
     2341 tcgcaatcat tcagctgaat gttaaaacca acggcagagc tggttgctcc agcctgcttc
     2401 aggctagcgg tacgaacctg tcccaactga acggtttgat caacagagcc tgcatcaact
     2461 gcgcaagcgg cgttaacaac ttccccttta aagtgaatgg tcccaccatt taccgtcgta
     2521 gtatcggcca gagccgctgc ggaactgagg gacagagccg acagaacaac gattgccaga
     2581 gttttaattt tcatgctgct ttcctttaaa aaaactattt ctaaatcgac atgggcagtc
     2641 gttctgtaca ctttgttttg tcaacgagtt tgactgccaa cactgcacag ttttccccca
     2701 aaagatgaaa catttggggc cattttgact catagaggaa agcatcgcgc acaaactttt
     2761 tcagtttatt tgttggctta atattctatt attatcttta tttatagatt tttatattgc
     2821 atgaggtggt ttttggagag aagaatgagg aagatgcgtc gagccacaga aacgttagct
     2881 ttacatatag cggaggtgat gtgaaattaa tttacaagag aaataattta catatcaaat
     2941 agttagatgc tttttgtcgt tttttaatat ttttatgctt gagaaaaaat acgtaactta
     3001 tttatgatat ggacagtttg gccccaattg tcttgtgttt atttgttttt taactttatt
     3061 atcaataagt taaatcaaac ctcttctctt tttaattttt cgtttatgag attatttctt
     3121 tcccataatc cggcaaaacg agcagcatta ctggcggtat aacgcacagt atggcgaata
     3181 tttcgatgcc cgagataatc ctgaattaaa cgggtatctg caccacgctc cgccagttca
     3241 taaccgcaag catgccttaa catatgagga tgagtctgcg ttacggttcc agcttcaata
     3301 ccggcatcgc gaataatgcg ataggcctgc tggcgagaaa gccgactccc gcggcgagaa
     3361 ataaatatag cgtcggtccg gtcagcgcct ttccagttag cacgttcctg ggtccagcgt
     3421 tccacggctt cacgctcatc aaaacgtaac gggtgaacgg tagaaaatcc gttcttcagt
     3481 cggcgaatat ttattcgacc ttcattaagg tcaaggtcct gataatgcag atcaagcagt
     3541 tcactaatac gcatcccatg ccgatatgcc aacagaataa gacaataatc tctggctccc
     3601 gttgccccgt aacaaaccgc ctgcatcatg gcctgaactt ctttaccggt aagataacga
     3661 cgtttactca caatagtagt actcctaact gagatatatt tgaatgaata cctataggaa
     3721 acctcaatcg gtcaaaatta gcccaataga taaaaagata accacacacg aagttatgtg
     3781 gctatcagtc aattacatta aacatcatac aaaaataaaa tatcactgat gtgacaacat
     3841 ctattttcat tgatttgatt caatataaca atagattaca gaggattaac ttccagttac
     3901 ttaaaaaata aagaaaaaag tgtggaaaca ggagaattta agtgatgtgt aaacttatga
     3961 atgattttgt ttatttattt tgaagaacat aaatcattag attttgtatg ggtatggtta
     4021 acttaatgtt tattatgata attaagaatc acacttacaa taacatcaat taataaatgg
     4081 ttaatcattt ttgatatatc gtaagaataa tgtagttttt aacaccatcc ctggtatctc
     4141 aactatctct ataaaacagc atgacgctgt cgtcctctgg ctctatccca gatgccgtaa
     4201 aaacgccctg cattgctggc ggtataccag acagtatgac gaatattgcg atgcccaaga
     4261 taatcctaga taagtcgcgt atctattccc atattcgcca aagcaaaacc acacgaatag
     4321 cgtaacatgt gcggatgaat ctccagtgac aacccggcat taccaccaga agtcgagata
     4381 atatggtaaa actgttgccg agaaagcgga ttccccttac gtgataaaaa tacccactcg
     4441 ctctcagcat gcggataaga agtacggata ctcaaccagt tttttaaagc ctgaacttct
     4501 ttgttcaata gcgggtgcgt tgttgaaaag ccttttttta atcgatggat atatatacac
     4561 tttgctttaa gatcaatatc cgaaatcctc aatcgacaaa tttcactcgc ccggaaacca
     4621 tgaataaaac aaagcaaaat cagacaataa ttacgtgttg catgaggccc ggtatttgct
     4681 gctttaagga gtgactcgat ttcactatgg gtaaggaagt tccttttttt gttatcagcc
     4741 ttattcctca tcgttttccc ttataattac agacgcgcac tagctgtgct gggttagcaa
     4801 taatccaaca tttttatcct gattatgttt ataaaagcga acgtttgctt aataacttac
     4861 gtaagtggac cagttcttct gagcgaactt aaatggagta gcgatagtta cttataataa
     4921 ctttgccata caaacgccat gctcttgcat gctatgtacc tttatatatt tatcaatctg
     4981 atacgaatct aataataacc ctcatcatta atgcaactaa tcttatctat atatcatgtg
     5041 atatgttctg taacaagtaa aatcaacata aaaaattcag caaaatttaa tatttgttaa
     5101 actagccgtc tcaaatatga cattattagt taactgcatt tgccacaaaa tggctatttc
     5161 atggaacgca gtaatttctt agtggctatt atcatgctaa ttcttagatc cctagcgatt
     5221 attcctgcct ggttcgtcag cttaggccat gagattgtta cattcctttt tatcctggag
     5281 atatggggga acgcaggcga cgctttttca tctacatccg ttggtaaata tagctttggt
     5341 cactacgact taagccttcc tcaatcacag ctattcgccg ggcgatacgg caaacaaact
     5401 aagtgaaaca tcatcctgaa aacaccaaca tcaacaagcc tctccagatc gacatcagga
     5461 gtgaccagtt acaagccaca aaccaagaaa catatttcat gatgagaatt atgctcagcc
     5521 tgatggcgag taaaccctta ttttcaccac tctcactgag ttagcttgca aagctttctt
     5581 tgtttagatt ctaaataaat tatttataaa acgtatagtt aaaagaaaat ctttagtttt
     5641 tgaagaccac cgcaagtgtt cgtctggctt cacatggcat cttcttctta gaaaaagatc
     5701 gacatatttt gtgacgcgaa ttgcaaatct ggttttgttg tatggattgc gtgatttttg
     5761 atctggtata acaggtataa agatgcacca cgatagtcaa tgagactggg catctcgtaa
     5821 tctatggcaa acatcacttc agttctttca catcgggtga tgaaaatgca cttcagtctg
     5881 aaaggaatat gaaaatgaga tcaacagaca ctctatttta tgactctggg taaaatgggt
     5941 tgagtaagtg atataactta cgaatattca aatcaattaa aaatcagaag agattttata
     6001 ctcaggtatt taatctggat ctctgtttat ttaaataatg ttaaaagaga attttcacag
     6061 gagaccttat acaaaaaata taaaatacag ttacccgttg ccaaagacac tataagcctg
     6121 gcaaaaaaat attacacaac ataaatgcta attgtttatg cgggctttgt attgctttct
     6181 gtatcctaca aatgagtgaa atttatgaaa aaggctaaaa tactttctgg cgtattatta
     6241 ctgtgctttt cgtccccatt aatttctcag gctgcgacac tggacgtacg tggtggatat
     6301 cgtagtggaa gccacgccta tgagactcga ctcaaagtca gtgagggatg gcaaaatgga
     6361 tggtgggcaa gcatggaaag taatacctgg aataccattc atgataataa aaaggaaaat
     6421 gccgcactca atgatgttca ggttgaagtt aattacgcaa ttaaacttga tgatcaatgg
     6481 acggtgcgcc cgggaatgtt aacgcatttt agcagcaacg gcacacgcta cggaccctac
     6541 gtaaaactgt cctgggacgc gacaaaagat cttaattttg gcattcgcta tcgttacgac
     6601 tggaaagctt accgacaaca agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt
     6661 tgggatggat atgtcactta ccatattaat agtgatttca ccttcgcatg gcaaacgacg
     6721 ctatacagca aacagaatga ttatcgctat gcaaaccaca agaaatgggc gacggaaaat
     6781 gcctttgttc tacaatacca tatgacgccc gatattacgc catacataga atatgactac
     6841 cttgaccgcc agggtgttta caacggcaga gataatttat cggaaaacag ttatcgcatt
     6901 ggtgtgtcat ttaaactgta gtagacagga gacagtcaca atgaataaaa ctataatggc
     6961 gcttgctatc atgatggctt catttgccgc aaacgcgtct gtattaccgg aaactcctgt
     7021 gccatttaaa agtggtaccg gagcaattga taacgacact gtctacattg gtttaggtag
     7081 cgcaggtaca gcatggtaca agctagatac acaggccaaa gataaaaaat ggactgcgtt
     7141 agctgcattc cctggtggac cgagagagca agcaacctct gcatttattg atggcaatct
     7201 gtatgtgttt ggcggcattg gcaaaaacag cgagggattg actcaggtat ttaatgacgt
     7261 acacaaatac aaccccaaaa ccaatagctg ggttaaattg atgtcgcacg cgccgatggg
     7321 catggcgggt catgtgactt ttgtacacaa cggcaaggct tatgttactg gcggtgttaa
     7381 ccagaatatc ttcaatggct attttgaaga tctcaacgag gctggaaaag attcaaccgc
     7441 tatagataaa atcaacgccc actattttga caaaaaagca gaagattatt tcttcaataa
     7501 gtttctgttg tcttttgatc cctcaacaca gcaatggagt tacgctggcg aatcgccctg
     7561 gtacggaacg gctggtgcgg cggttgtgaa taaaggtgat aaaacctggc ttattaatgg
     7621 cgaagccaaa ccaggattgc gaacggatgc cgtatttgaa cttgatttca ccggtaataa
     7681 tttaaaatgg aataagcttg atcccgtctc atcaccagat ggcgtcgctg gcggttttgc
     7741 ggggataagc aatgattctc ttatatttgc tggaggggcc ggattcaaag gttcacgaga
     7801 aaattaccag aacggtaaga actatgcgca tgaaggcctg aaaaaatcat atagcactga
     7861 tattcatctt tggcataacg ggaaatggga taaatcgggt gaattatcgc aaggtcgggc
     7921 ctacggagta tcattgccct ggaataatag tttattaatt attggcggtg aaactgcagg
     7981 cggcaaagcg gtgacggatt cagttttaat ttctgtgaag gataataaag tcacagtaca
     8041 aaactaacgc ttcagggccc cggtaaaggg gccctgctat caatttgtca tttgtattac
     8101 gcgaaaatta tatgaacgca ataatatcac ccgattatta ctatgttctt accgttgctg
     8161 gtcagtctaa tgccatggcg tatggcgaag gactgccatt accggacagg gaagatgcgc
     8221 ctcattccag aattaaacaa ttagcgagat ttgcgcatac gcatcccgga ggcccgccat
     8281 gtcactttaa cgacattatt ccactgactc actgcccaca cgatgttcag gatatgcagg
     8341 gttatcacca tcctctggca acgaatcatc aaacacagta cggcaccgtt ggccaggcac
     8401 tgcatattgc acggaaatta ctgcccttca ttcctgataa tgcagggatt cttatcgttc
     8461 cgtgttgccg tggcggatcg gcttttaccg cgggcagcga agggacatat tcagaacggc
     8521 acggagccag ccatgatgct tgtcgttggg gaacggatac tccgctgtac caggatttag
     8581 tcagcagaac gcgagccgca ctggcaaaaa atccgcagaa caaattcctc ggcgtatgct
     8641 ggatgcaagg cgaatttgac ttaatgacca gtgactacgc gtcacaccct caacacttta
     8701 atcatatggt tgaagccttt cgtagggatc taaaacaata ccattctcag cttaataata
     8761 ttactgacgc gccgtggttt tgcggcgata ccacctggta ctggaaagaa aatttcccgc
     8821 attcgtatga agctatttat ggcaattatc aaaataatgt tttagccaat attattttcg
     8881 tcgacttcca gcaacaaggt gaaagaggac tgacgaacgc gcctgatgaa gacccggacg
     8941 atttaagcac gggatattac ggttcagcgt accggtcacc ggagaactgg acgacggcac
     9001 tgcgaagcag tcatttcagc acggcagccc gtcgggggat tatttctgac agatttgtag
     9061 aagcaatttt gcagttttgg cgcgaaaggt gagcggggag tttattcatg ggggatgtcc
     9121 ttgttcgttc tcagagtaga caacaaaaaa caaatgagat caaggtaata tctcttcact
     9181 tgcgggctcg aacagcaggc aattgccaat ataataatct ggcacaaggg tgaaa
//
LOCUS       DAJTUI010000135         9216 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTUI010000135 DAJTUI010000000
VERSION     DAJTUI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9216
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.135"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(252..443)
                     /locus_tag="OO464_003599"
                     /pseudo
     CDS             complement(252..443)
                     /locus_tag="OO464_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            441..765
                     /locus_tag="OO464_003600"
                     /pseudo
     CDS             441..765
                     /locus_tag="OO464_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(795..3707)
                     /locus_tag="OO464_003601"
     CDS             complement(795..3707)
                     /locus_tag="OO464_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228605.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3710..3826
                     /locus_tag="OO464_003602"
     CDS             3710..3826
                     /locus_tag="OO464_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6228606.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            4190..5032
                     /locus_tag="OO464_003603"
     CDS             4190..5032
                     /locus_tag="OO464_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6228607.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            5035..6123
                     /gene="capU"
                     /locus_tag="OO464_003604"
     CDS             5035..6123
                     /gene="capU"
                     /locus_tag="OO464_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR6228608.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            6128..7078
                     /gene="virK"
                     /locus_tag="OO464_003605"
     CDS             6128..7078
                     /gene="virK"
                     /locus_tag="OO464_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6228609.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            7143..8087
                     /gene="lpxM"
                     /locus_tag="OO464_003606"
                     /gene_synonym="msbB"
     CDS             7143..8087
                     /gene="lpxM"
                     /locus_tag="OO464_003606"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6228610.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(8241..>9216)
                     /locus_tag="OO464_003607"
     CDS             complement(8241..>9216)
                     /locus_tag="OO464_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR6228611.1"
                     /translation="CLKKEQMVVSAIASIPQLHRGDRVSDVARTLCCARSSVGRWINW
                     FTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGDFGYQRSRWSTELLAIKINE
                     ITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEKMAAIHKALDECSAEHPVFY
                     EDEVDIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSA
                     LFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNH
                     VERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT         2425 a         2123 c         2213 g         2455 t
ORIGIN      
        1 acgtgggtgg actggtataa caatcgacga ttgcaggaaa ggctgggcca catccctccg
       61 gcagaagcag aaaaagctta ttatgcttcc atcggaaacg atgatctggc agcctgagtt
      121 cacagataaa acactctcca ggaaacccgg ggcggttcaa agggccttcc cgagagtggt
      181 aaaggagagg ccctggaaat accccacagc cccgaaaaag agccagtcag ttgcttaact
      241 gactggcatt aaggccgccg gggacgtttt tttaccaccg ggagtttggg cataacggtg
      301 ctttcagtaa gtacgacagg tactgtggag gcacttccgt aaagaagggc atcaagctcc
      361 tgctccgaca tacctgcagg caaaggccat gaaagccctg atttccggaa gcgagcgaac
      421 agtccacaaa ctgttgaacg tggtgtcagc ggcggacagg gataagccct cgtcagcagt
      481 tccgtcagca ctgcgacagc gttctttacc cggtcaaaac agcgttgcgg tgcaccacgc
      541 ctgacggatg aactgcgtgc tcagggttac cactttaacg taaaaaccgt ggcggcaagc
      601 ctgcgccgtc aggggctgag ggcaaaggcc tcccggaagt tcagcccggt cagttaccgc
      661 gcacatggcc tgcccgtgtc agaaaatctg ctggagcagg atttttacgc cagtggtccg
      721 aaccagaagt gggcgggaga catcacgtac ttacgtacag atgaagttcg gttacatcct
      781 gtcagcacgg aacctcacgc tttttgagcg gcacgacgga tcccccccac ccagtatccg
      841 acagactttt cactgcgttc gtaaaaaccc ttcttccctc ccagcatatc cactaattcc
      901 ggtgacatat cctgcagata agtctgcaaa tcatgcccca gatcttccgg tgactgcaac
      961 tcataaaccg cgcgccacac atcacccttc cttattttaa gccccgggtt aagaatatac
     1021 tgtccgtggt tcagcctcag aaaaagcttt tttccataag gatttttgct gttaacttca
     1081 tgttgagaga gctggctgct gatataactc cttcgtttgc ggcgttccgg cagcaacgct
     1141 tcaggaagat gaaccacaca ctctgccagt cgtgccgcac tgaaggcttc cgaaaaaaac
     1201 agagcacttc ccagatgcgt gtggaacagc gcctgcatca ggatcaccag aaacattgcc
     1261 atctgccggt tgtccagttt gatgaggcgt ccttccacct gcagtgacag actttcaggt
     1321 ttcagcaggg tatacaactg cgcacttttc tgttgcgcat aacggggcgt actgaccgcc
     1381 gctgacagaa cttgatgata agccgccagc ccgtcattac cggtcagcaa gggatctgca
     1441 ccacgctcca gcagtaactg aaccagtgca atattacctg cccgggctgc tgacatcagc
     1501 ggagtaagat taaacggcga acggtgatca acaccataac gttcaatatc ccgcaacacg
     1561 gcaaccgggt tattcattga gtatggccgg aaatacttca gctccagtaa ctgcagagtc
     1621 ttcgttctgg gctgccttgt gggcttaaaa tcttcccggt acagtgcatt atacagcggg
     1681 aacagggaat aaatcagact gtactccagc gcctgcagtt gtatggtttt atcggctgta
     1741 cctgcgagga tatgctgttt ccactgtcgt gcatcctctg cggtgataat ctgccatggc
     1801 atctcagatg tctgcaggat ccggctacgg atggtgtccg cctgctcctg cttcccctgt
     1861 ttttcaagca gatgtgcggt tttctgccac tcatcacggc tggaaatctc ttcctgcaga
     1921 ttcagaggct cttcctgatg cgtgagcgca agcagactcc acagaggatg ctccacctgc
     1981 tgttcaacaa gatacacatt gtgtttagca cgggtgagcg caacatacag cgcgttggta
     2041 aaaaatttat agatttccgc tgaacgatcc tgcctgtccc gcggacggga ataacgggcc
     2101 tcgccctcaa gatcagccgg tgtaagaccc tcgcaaatat catcaaatgc ctgccgggca
     2161 gcgcttacta tattgtaaag gatcaccgtt tcgtattcca gccccttaac ctcctgaacc
     2221 gaaaagacca gcggcgtact gaaccagcat cgcgcctgtg ctttctgttc cggatgcatc
     2281 acaatgaccg ctacacgatt cgaaagtgag gttttggcat tcagttcctg ctttgtctct
     2341 tccctatcgt ccagcaaccg gatggtccct tctgcctgac cgcagcttct gacaaaatgg
     2401 tgactttccc gatcaatagc actaaagcgg acctgtttga gtctcagcaa ccggttagcc
     2461 agtgcagtga cgtggtgccc gttacggtaa ttcgcctgca ggatattaac ggttgtgtcg
     2521 tttccctgtt gctgccggaa aaagagactt ttgagcgaac tccatgagaa aaagctcgga
     2581 tgaactatct gattggcatc accacagagt aaaaactgac cgggatgacg tagcgttttc
     2641 agtaccagtt gcagttgaac aggcgtcata tcctgaactt catcaacaaa aatgacatca
     2701 taacggggag ttgccaacga aagatactga tgactgagca gattactgtc atactgatgc
     2761 gactgtttca gccatgcgat ataacgttca aacaggacat agaccgtcgg gcggtcctcc
     2821 atgccataga gcgactgccg tatccccagt gacagatacg cctcccggga cagcggccca
     2881 ttcccggagg caaccgcacc gatcacgccc ctgaactcct catacagggt atgggcagcc
     2941 cccagtgcgg cgatagccct gttgcggggc agccagtcgc tgaacgcact gaaagtcacc
     3001 tccctgcctt ccggaatgcg cagagtttcc agaaattccg tcagcgaaag aaagtcaata
     3061 ttctgtacct cactcccttc ccctgaagcg tcatagagag tacgcgcttt ttccaccaga
     3121 aatgaggaaa ggctgagata cagtatatca ccagcagcct gcttcatttt ttccagtacc
     3181 agcgccgtct taccacttcc cgccgagcca acaatcacca gcggtagcgg gtgttcgtac
     3241 agtgcctgct gatcatcatc aaaggacagc attttatcca ggcggtggaa acgcccatga
     3301 gacggattga tatacgtcag cgcttcagtc gcgatatcaa caggatccgg cactggttgt
     3361 tgttgtaagc gcccttcatc aatcaccacg tttctgcgaa gaaaacgtga agtgttatag
     3421 gcatgattgc gaatatattc cagaacaagg cagatagttt caccgtggta gcgatacagc
     3481 gaaaacagta atcggtcccg gatactgagc cgggcgcggt aaagattggt gtcaatttta
     3541 cgaacatcag cctgaacaaa gttatcggtg gcaatagcct gtgcaaattt tttataaccc
     3601 gggatcgtgt caggattcag agtatgataa agacagattt tcatggctgc cggtttccgt
     3661 cgtgcaaaaa acatgctgat tttacgaagg aatgccttac tgaacaacct tgcccggaat
     3721 accccccgaa atcagatgga acaatcagaa aaatgctctg cagttatgga ataaagaagg
     3781 tggggtggta cgccgggagg gcgatccgct ggaaatggcg cagtagctgc aggcctgtca
     3841 tgatgcagga atagaagttc gtgtacaggt taatgaaagc gtcaccccgt aaataaactc
     3901 tgcgctgaaa ttcctttttt tcattggagt acctgtgttg ttctgaggtg aacatatcac
     3961 ctctgttcag gtagccaaat tcagtgtgcc acctcaaggt tcttagtgag tcgtttttaa
     4021 aagatatgat aatatatcat tgtttatgaa ttgttgatgt ttaatttagt aaattatatc
     4081 aaatatgcca tgggtttgct atgttattca tccagtatct ggaaataaaa tgcaccagct
     4141 ttattgggtt ttccttttgt ctctgcttgc taggtgccag aaactttaaa tgttaaacga
     4201 agggggcatt ttgttcaaag ccaatcatgt tccggtatta atgtatcatc atgtcagtca
     4261 ttgccccgga ctggttacac tttctcccgt gacattccgg aagcaaataa aatggttggc
     4321 tgaaaacaac tggaaaacac tgtcatcaga tgaactggag tttttttatc ggggcggaaa
     4381 actcccccgg aaaagcgtta tgttgacatt tgatgatgga taccttgaca actggtttca
     4441 ggtatatcct ttattaaaag agtttaatct caaagcacat atttttttaa ttaccggttt
     4501 tattgggaat ggtccggttc gtcactctcc tggaaaggaa tattctcatc gcgattgcga
     4561 acatcaaata gcgacaggga atgctgataa tgttatgctt cgctggtctg aagtaaacga
     4621 aatgttgcaa tctggtctgg tcgaatttca tgttcatacg catactcata cacgctggga
     4681 taaaaagttc agttcacgag aagaacagtg taaacatctt cggcaggact tgttgagcgg
     4741 gagggaatat ttgaaagaaa tgacaggaaa atgcagtaaa catttgtgtt ggccggaagg
     4801 ttattataac aaggattata ttcaggtcgc tgaagaactt ggattctatt atttatatac
     4861 aacagaaaga agaatgaatg ctcctgctaa aggaactaca cggataggaa gaataagcac
     4921 caaagaacgg gaaagttgcg catggctgaa aagacgactt ttttattata caaccccgtt
     4981 tttttcatcg ttgctggcct ttcataaggg gccaagatta ccggatgatt aataatgaat
     5041 atactattta cggaatcatc accgaatata ggtggtcagg aattacaggc tgttgctcaa
     5101 atgaaggccc tgaagaaaat ggggcattca gttctgcttg tctgcaggga aaacagcaaa
     5161 attgcttttg aagccagtaa attggggatt gatatcacat tcgcgttatt tcgaaacagt
     5221 cttcacatcc ctactgcatg gagattactc ggaatagttc atggttttca gcccaatgca
     5281 atcgtttgtc acagtgggca tgatagcaat attgttggtt tagtacggtt attcactagg
     5341 aaacatccat tcagaattat caggcaaaag acatatttga cacgaaaaac aaaagttttt
     5401 tcaataaatc atttttgcga tgaggtgatt gttcccggaa caagtatgaa gacacatctg
     5461 gagcaggaag gatgtcgaac ccgggttact gttgtgcctc caggctttga tttccagaaa
     5521 ttatacgttg attcccgaaa cagtttgcct ccaaatgttc tttcttggct ggcgtcccga
     5581 aggggatgcc ccgttattgc tcaggtagga atgttgcgcc cggaaaaagg gcacgaattt
     5641 atgttgaatt tactgttcca tttaaaaatg aatggacgac agttctgttg gttgattgtg
     5701 gggtctggtt cgcctgaact gcgggagcat ttacagtatc agattgacag tatgggcatg
     5761 catgatgatg tttttattgc tgacaatgtt tttcctgccg cccccgtata tcgggttgcc
     5821 agtctggtgg ttctgccttc agaaaacgaa tcgtttggta tggtactggc agaagcatcg
     5881 gcattttctg tgcctgtact ggccagtcag attggtggaa tccctgatgt tattcagaat
     5941 aaccagaccg ggacattgtt accagcaggt aataagcacg catggatgtg cgccctgaat
     6001 gattttttta atgaccctgg gcgtttttat cagatggctc gccaggcaaa acaggatata
     6061 gaagagcggt ttgatattaa taaaactgcg ttaaaaatac tcacattagc gaagcacaag
     6121 taacaatatg ttttctgtaa gtaacttatc atttatcggt ttccttaaaa ggattgtttt
     6181 ttcctcagat tcactcccgg ggaagtggga acacagaaaa ttccggttca tgtacatttt
     6241 gcgatgtgct ataaatccgg ttgccagtat tcgatattat tacgaactgc gttccttgca
     6301 gtgcattgag gatattctgg caatacaacc cacgttgcca gcaagaattc atcgacctta
     6361 tttgcataaa ggggggcgag cctggtcccg ggggcaatat attcttgagc attatcgctt
     6421 tgtgcaaaat ttgccagaaa aatattctga atttttgttt ccacaaaaat cggtatcatt
     6481 agtccaattc atcgggaaag atggagagga ttttgatatc cagtgttctc ccagtggttt
     6541 tgaccgggaa ggagagttga tgttgtcatt gtttttcaat aaaatagtga ttgcccggtt
     6601 aacattttca gtcattctga ctcaaaatgg acacaccgct tttattggcg gattacaggg
     6661 agcccccaaa aataccggac ctgatattat tcggtgcgca acccgggcct gttatgggct
     6721 tttcccaaaa cggattattt ttgaagcatt ttgtgcgttg atgaaagctt gtaatgtctc
     6781 tgagtgtctg gcagtcagtg agcacagtca tgttttccgg caattgagat actggtatca
     6841 gaaacgcaaa acctttgttg ctgtctacag tgatttctgg gagtctgtag cagggaaaac
     6901 ctgtggtgac tggtacaaat taccaacaca ggtggttcga aagccgctaa gcaatatagc
     6961 tagtaaaaaa cgctctgagt atcgaaaacg atacgctttg ctggattata tccatgaaac
     7021 tgctatccgc tctttggatg catatcctgt aaactcagaa catcaggact taaattaagg
     7081 tttgatgtat tctctgaata aaatattaat gatgattttg gtaggggcat tcgcactaaa
     7141 taatgaaaaa atacaaatcc gagtttattc ctgaatttaa gaaaaattat ctttcccctg
     7201 tttactggtt tacatggttc gttttgggaa tgattgcagg tatttcaatg tttccccctt
     7261 cattcagaga tcctgtcttg gccaaaatag ggcgttgggt gggcagattg agcagaaaag
     7321 ctcgtcgcag ggcgacgatt aatttatcgc tttgtttccc ggaaaagagt gatacagaac
     7381 gggaaataat tgtcgacaat atgtttgcca cagcattgca atccatagtg atgatggcag
     7441 aactggcaat tcgtggtccg gaaaagttcc agaaacgagt gttctggaaa ggcctcgaaa
     7501 ttcttgagga aattcggcac aataacagaa atgtgatttt tctggttccc catggctgga
     7561 gcgtggatat tcctgcaatg ttgctggcag ctcaggggga aaaaatggcc gccatgtttc
     7621 atcagcaacg aaatccagtg attgattatg tctggaattc tgtacggcgt aaattcgggg
     7681 ggcgcttaca ttcccgggag gatgggataa aaccatttat tcagtcagta cgccagggat
     7741 actgggggta ttaccttcca gatcaggatc atggtcctga atacagtgaa tttgctgatt
     7801 tttttgcgac ctataaagcg acattaccaa ttattggacg tctgatgaac atcagtcagg
     7861 ctatgattat accgcttttc ccggtttatg atgaaaaaaa acatttcctg actattgaag
     7921 ttcggccacc aatggatgca tgcattgcca gcgcggacaa taaaatgatt gcccgacaaa
     7981 tgaacaaaac agtggaaatt ttggtggggt cacatccgga acagtatatc tgggttttaa
     8041 aattgttaaa aacgcgcaaa tcaaacgaag cggacccgta cccttgaaat tataaagtac
     8101 aggtatttcc actagttgtt tcttacaggt taccaatcga aacacatccc ccttccgtgt
     8161 gttgcccgtt tggcacggag gactgtgtag atgcagttcg ttaaatttaa tttatgccta
     8221 aatagctgcg cttaataccg ctacactttt gccagcccat gtttgcctcc ggggaatggg
     8281 ctgacggttt ccataaaatg gcgaactttt ttcaacagtt gccacattga gcggcactga
     8341 tgattacgcg ttattgtgtc gtgaagtgcc tgccatagcc gttcaacatg attcacccat
     8401 ggcgagtaaa ccggctgata aatgcccctg aacttcgggt tctccttcag ccagctctgt
     8461 gtttcccggc ttttgtggat aatgtagttg tccacgatca gcgtgatggt tttcgcacga
     8521 cggtatgtcg ctttaagccg cttcagcagg ctgatgaaca gcgccgaact tttgctgttg
     8581 ccgcccacat agctgacttt acctgtcccg ctgtgcagcg ctccggccag ataatatttt
     8641 tcattctgtc ccggcgtgac cacccgtttt tgctgtccgc gcagttgcca gtccgcaccg
     8701 attttgggat gaagatggat atccacttca tcttcataaa agaccggatg ctctgcgctg
     8761 cattcgtcca gtgctttatg gattgctgcc atcttttcat ctttatgcgg gtcacggata
     8821 cgcagagttg gcgcagccct tcgccacaca atccccgcag acggcaacca gcggcgaacg
     8881 gttccggcat ttaactggca gccggttatc tcattgattt ttattgccag cagttctgta
     8941 ctccagcgtg aacgctggta gccaaagtcg ccgggagaat gttttaccag ctcacgtaac
     9001 agtgtgcaga tatgctcaaa cggccagcga cgggcacgcc cggcaggtaa tgatttcagt
     9061 ccctcaacac ccgactgcgt gaaccagtta atccagcgtc caacagagga gcgggcgcag
     9121 cagagcgttc tggcaacgtc gctgacacgg tcgccccggt gcagctgagg tatactggca
     9181 atagcggaca ctaccatttg ttcttttttt aagcag
//
LOCUS       DAJTUI010000136         9182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTUI010000136 DAJTUI010000000
VERSION     DAJTUI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.136"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(43..1458)
                     /gene="phoA"
                     /locus_tag="OO464_003608"
     CDS             complement(43..1458)
                     /gene="phoA"
                     /locus_tag="OO464_003608"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR6228612.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1559..1819)
                     /gene="iraP"
                     /locus_tag="OO464_003609"
     CDS             complement(1559..1819)
                     /gene="iraP"
                     /locus_tag="OO464_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6228613.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2001..2204)
                     /locus_tag="OO464_003610"
     CDS             complement(2001..2204)
                     /locus_tag="OO464_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228614.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2282..3376
                     /gene="ddlA"
                     /locus_tag="OO464_003611"
     CDS             2282..3376
                     /gene="ddlA"
                     /locus_tag="OO464_003611"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6228615.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3400..3612)
                     /locus_tag="OO464_003612"
     CDS             complement(3400..3612)
                     /locus_tag="OO464_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6228616.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            3872..4180
                     /locus_tag="OO464_003613"
     CDS             3872..4180
                     /locus_tag="OO464_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6228617.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4239..5333)
                     /gene="yaiW"
                     /locus_tag="OO464_003614"
     CDS             complement(4239..5333)
                     /gene="yaiW"
                     /locus_tag="OO464_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6228618.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5346..6566)
                     /gene="sbmA"
                     /locus_tag="OO464_003615"
     CDS             complement(5346..6566)
                     /gene="sbmA"
                     /locus_tag="OO464_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6228619.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            6918..8075
                     /gene="ampH"
                     /locus_tag="OO464_003616"
     CDS             6918..8075
                     /gene="ampH"
                     /locus_tag="OO464_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6228620.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8076..8579)
                     /locus_tag="OO464_003617"
     CDS             complement(8076..8579)
                     /locus_tag="OO464_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6228621.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(8667..>9182)
                     /locus_tag="OO464_003618"
     CDS             complement(8667..>9182)
                     /locus_tag="OO464_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6228622.1"
                     /translation="SHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDAGYV
                     TPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPYFKL
                     AYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQD
                     WSANVGVKYTW"
BASE COUNT         2288 a         2344 c         2192 g         2358 t
ORIGIN      
        1 aaaaaaccac ccggcagcga aaattcactg ccgggcgcag ttttatttca gccccagagc
       61 ggctttcatg gtgtagaaga gatcggtctg gtcggtcagt ccaacaacat tggcggcatg
      121 cgggccatac gccgcaatac gcaactgact gccggtatgt tcttgtgaat cctcttcgga
      181 attcccgtaa ctcatcacca tcactgcgcc atctttagta tttagcgcct gagtgaggcc
      241 cggcgctttg gtatccggcg caacaacctg gctggcgtgg gcgtgatcag cggtgactat
      301 gaccagcgtg ttaccctcct ttttagcgaa ttccagcgcc cgttgtacgg cttcatcgag
      361 atcgaccgtc tcgccaattt gcccacaagg gttcgcagcg tgatcctgtt tatcgattga
      421 cgcaccttca acttgcagga aaaatccttt ctcattttta ctcaacaatt caatggcttt
      481 gtcggtcatc tgtgccaggg ttggtacact gtcattacgt tgcggattag gcgtataggt
      541 gactgcgggc ttatcgatat tgccgtggta cgttgctttc ggccctagcc agcgcactgg
      601 catattgccg tcagcaaaca ggccaagcag gggtttttgc tgattcgctt ccgtcaccga
      661 attcagtgag gcagcatcgt tcaccaactg ataaccacgc gcctgtgcct gttcacgcag
      721 cgtttttccc tgccattcac cagcggttgc cgtttcagca aaggtttttg cgccgccgcc
      781 aagcgtaacg tcggcacgag cgttaagcag ctgttcggta atcgatcctt ttccgccttt
      841 ttccagagcg ttacccggac atttttcact ggtcgcgctc ggaccgtagc atttgcgcga
      901 agtcacatgt gccaccagcg cagcgggcgt ggcatcctgc aactctgcgg tagaaacgtt
      961 accggtcgcc agacctgcgg cttttgccat ttccagaatc gttgggtgat ctttttcgtg
     1021 aatatcgacg cccagcgcgc cgttataggt tttgacatcg gttgaccagg cggttgctga
     1081 tgcagccgag tcggtgacgt agtccggttt gccagttttt ttattcagcg catagtgagt
     1141 gtattgcccg gtaagcggta aggcatcgat acctttaaaa aagccgcccg caccttcggc
     1201 ataattacgt gcggcagtaa tttccgaatc ccccatccca tcgccaatca gcaaaataat
     1261 attttttgca ggtttatcgc taagagaatc acgcagagcg gcggtctgat cacccgttaa
     1321 gcggcgagca ccgccgggtg cagtaatatc gccctgagca gcccggtttt ccagaacagg
     1381 catttctggt gtccgggctt ttgtcacagg ggtaaacagt aacggtaaga gtgccagtgc
     1441 aatagtgctt tgtttcactt tattttctcc atgtacaaat acattaaaaa ataaaaacaa
     1501 agcgactata agtctcggcc gtgacaactt tatgacagct gttgaaaaga ttaacttttt
     1561 actgacaagg atgcttcaat aacttcttta cgtaatcgcg cagcagctct gtatcgtcgt
     1621 caggaatgct ggcatcgggc tttacctcgt acagcgcccc ctctacctga tcaatcaacc
     1681 gctgttggtc attttgcgcc atattgcgaa gcattgcagt gacgataatc tccaaagctt
     1741 ctacctgcgc acacagttct ttcgactctt cttctttttg ggcaagctta aataacaact
     1801 cagcaatgag atttttcatg tctgtatttc cttatccaaa gtatggagaa gttaacatta
     1861 tgtttcacaa cttcatagtg tctggaagta ttatttttat gacaaaggaa atactttgcg
     1921 caaatatttt ttgtttgatt gccagcgaaa cgtttctctt atttaaataa ttacccacac
     1981 aaaatatagg cttttaatta tcaaccttta ttataaataa aagctatgaa atatattctc
     2041 ttcgttaact tataattaag agaaaaaaac tcaccgcatt gccatttaag ccaggaataa
     2101 attagcttcc cgttcatcat ttttgtgatc ttgcgcacat ctttgagata acgcactttc
     2161 ttcaccagta cgttgcacgc tttgtcagca gaagattgca acaatctttc agaaggccgc
     2221 gaatcccccg ttttgtcgtg gtaatgtatg cactttgtgt tagacgggat aggtttttaa
     2281 gatggaaaaa ctgcgggtag gaatcgtttt tggtggtaaa tcagcggaac atgaagtgtc
     2341 tctgcaatcg gcaaaaaaca ttgtcgatgc cattgataaa agtcgcttcg acgttgtgct
     2401 gctgggcatt gataaacaag ggcaatggca cgtcagcgat gccagcaatt atctgctaaa
     2461 tgcagacgat cctgcccata ttgcgttgcg cccttcggcg accagccttg cgcaggtgcc
     2521 aggtaaacat gagcatcagc ttatcgacgc gcaaaacggt cagccgttgc cgacggtgga
     2581 tgtcattttc ccgattgtcc acggtacgct gggcgaagat ggttccttgc agggaatgct
     2641 gcgggtcgcc aatttaccgt ttgtaggttc tgatgttctg gcttcagcag cctgtatgga
     2701 taaagatgtc accaaacgtc tgctacgcga tgccgggctg aacattgcgc catttattac
     2761 cctgacgcgc gctaatcgtc acaacatcag ttttgccgaa gtggagtcta aactggggtt
     2821 accactgttt gtaaaaccgg ctaatcaggg ctcttctgtt ggtgtcagca aagtaaccag
     2881 tgaagaacag tacgcaattg ccgtcgatct ggcgttcgag ttcgatcata aagtgatcgt
     2941 tgagcaaggg atcaaaggtc gtgagatcga atgcgcagtt ctgggcaacg acaatccgca
     3001 agccagcacc tgtggcgaga tcgtactcac cagcgatttc tatgcctacg acaccaagta
     3061 cattgacgaa gatggcgcga aagtggtagt tccggcagcc attgcaccag agatcaacga
     3121 taagatccgg gcgattgccg ttcaggctta tcagacgttg ggatgcgcag gcatggcgcg
     3181 tgtagacgtg tttttaaccc cagagaacga agtggtgatc aacgagatca acacactgcc
     3241 tggcttcacc aacatcagta tgtatccgaa gctgtggcaa gccagcggtc tgggttacac
     3301 cgatctgatc acacgtttga ttgagctggc gctggagcgt cacgctgcgg ataacgcatt
     3361 gaaaaccaca atgtaatatt ttaacgcccg ataatattct tattgggcgt tttcagtttc
     3421 ttcatcgcga cgacgaataa tcaaccccgc cagccagaaa ctaatcaccc acgtcaccag
     3481 accgacggca taggtctgcc aaccttttgc ttcaaatccc agtaccccca ccacgccatt
     3541 aagaatgaat ataaggccaa tggcgaacgc atagtagtgc cagtcacggc ggatttttac
     3601 aggcagattc atggcagctc cgttaacgaa aaacaacatc cttgcatatt ctgtaacaac
     3661 cgtctgtggt gtagacaaca ttttcgtaat tattaataaa tttaacttaa ggttgccatt
     3721 ttgtgactca caacgaagat tcacgcttcg ggaatattcc caacatcaat ttaccatgat
     3781 tctgatattg acaatctaaa tgacctgtca gacttgccat aagccgctgg tatcacacca
     3841 gaagagaagc agatgacttc cggaggtttt tatggctgat ttcaccctgt caaaatcgct
     3901 gtttagcgga aaatatcgca atgcctcttc aacgcctggc aacattgcct atgcgttgtt
     3961 tgtgctgttt tgcttttggg ctggggcgca attgctgaac ctgttagtgc atgcgcccgg
     4021 cgtctatgag cgtttaatgc aggtccagga aacaggtcgc ccacgggtgg aaattggttt
     4081 aggtgtcggc accattttcg ggctgatccc gtttttagca ggctgcctca tttttgcagt
     4141 ggtggcgcta tggctgcact ggcgacatcg ccgccagtaa aatttacgtt gccagatgcg
     4201 gcctgaaacg ccttatctgg cctgacattc ctgtttgatc actgtttcat acaacgcgcc
     4261 cgccgttcgt ctacgcgctt cgcaaaccag gccgtagtca ggttgcgggt gattttcggg
     4321 ctttccagtt gaatgccagg caacatttcg cggggtagtg atttacccgt tttcgcttcg
     4381 gcaagttgat aaactttctt gtacagcgcc gtctcctcaa aagagaagct gtcacctttc
     4441 tctaactgac ggcgaatttc gctgtcgctc atccccagtt ttcccgccag tttgcgcgtt
     4501 gccagttcgg ttttcccggg ttctttactg tcgtaacgaa ttaaatcacc gtccagtgcc
     4561 agcttcacgc cgctggcctt actgactgcg ttctgaaacg cggcattacg gctggcgtac
     4621 cagccagcgt taaaatcagc aaagcggtat atcggtgcgc tatagctggc gggataattc
     4681 agtaaatggt aagtaccaaa ccacaacccg ccgcggcgac tgaagacttc ctgacgaact
     4741 gtaccgtcca ttttccacgg ataccctttg gtatgctgtt cggcaaaagc aatgctgacc
     4801 tgcatcggcc caccggtgcg caccggatta agcgaaccaa acagcgtctg ccccattggt
     4861 accatgctga tcagatcgtc aaaaatcgcg ctcaattgct tttctgtacg caccgaatct
     4921 aaccgttcgc tataactttt gccgtttggc gatttgattt ttagcgcggt atgaaccaga
     4981 aaagcaggaa tgtgcatccg ttcagcacga cggtcaatct cttgccaggc gatcttgctt
     5041 aagcccggaa cggccggatc tgcctgataa tttgactcct gctgcgccac cgccagcacc
     5101 gagtagacgt tttccagcgt cggtgccagc ccctgacttt caaaggtggt ggcgagatct
     5161 ttcgcccagg catcgcggtc tttcacgctc gcaggcatct tctgccgcac gacgctcgct
     5221 acatctatcg ctttttcgcc ctttttcaac ggttgcggag cctgactact acaccccgct
     5281 aaaaccagaa ccgccaacaa tgacagcgaa gatagatgat tgacgcgcga cattacttct
     5341 cctttttagc tcaaggtatg ggttacttcc tgaattttgt caccatccag ctcatgttca
     5401 aagctgcgca gacgtttgta gatagacatc aactcaacca gtgtggtcca tgagttaatc
     5461 aggtactgga aagcaccgcg aacctgacca aaaacgttgg taatctgcgt catcaggccg
     5521 agcgtaatcg taccggcaac aattgacgga aacagcaaga acaaaccgaa aacgttatcg
     5581 acctgcaaat agagaatgcg ggcgatgttg aaatacatat agtgaaaata gaggcgaaaa
     5641 tagtttttcc gtacggcgct aaacagctcg cgtaccgtag gcggcgtcgc gcgcgtggca
     5701 tcgtcttcac cataaaccag ctctttacgg taggcggcct ctactcgctg gtttttaaac
     5761 tccagccccg gcagtttgat ccctaccact gccagcaatc cggtccccat cagcgaccag
     5821 acgatagcgg caatcaccag accatacgga atgtgcccga taatcggcag ctccggcaca
     5881 tgcgcggaga gcgttaccag caccggcagg aaggcgatca acgtcatgat ggcgttgata
     5941 aaactgacgc ccatattctc cagcgttgaa gcaaaacgca tggtgtcttc ctgcacacgc
     6001 tgtgcggcac cttcgatatg acgcagttgt tgccagttcg ccatgtaata ttcgttcatc
     6061 gccgtacgcc agcggaacac gtagtgactg acaaagaagt tgttcagtac actgatcacc
     6121 accgcgatca gcgcaatccc cagaaagacg cccacttcgc ggtaaaattg ttcgatagtg
     6181 actttatgcg gcgaacttag cgcggtttga atcagatcat agaacggcgc ataccaggcg
     6241 ttgacggcga ccccgacttc caccaaaaac caagtgacga agataatcag cgcagtaccg
     6301 agtattgacc agtattgcca acggtgcggg ctgtagataa accagaaaaa tgcaaaaagt
     6361 cctacgcaaa caatgtagta agcgtaaaaa atcaggaaat ccaacgacca gaaacgcgcg
     6421 gcgctaatcg ggatctgccc ggaagcgccg gtgatacgtg ccacccagtc acccccaccg
     6481 gcttgccaga agataacggc aatcaatgcc caaacaaagg ccgagagaaa aaacgttccc
     6541 ggctttggga aaaaagactt aaacatatgc actcctgcta acttcttatc gttttaacaa
     6601 ctgctgtgta ttaaccgcat gaccgctaca aagcgtcagc caaatgaaac cgcccattgt
     6661 gacccatagc aaggcgctta gttcgccagc ctgaccataa gaattgtttc gacggatttc
     6721 acaaaccaaa gattgtttac ataagcggat taacacgcgc aggatcacaa atgcaggcat
     6781 tggtgagcag gaaaaagatc gtgtttacgc gccgttacgt ttttaacact attattgcac
     6841 taattcgccc tttgcaatcc atcaatgagt agtataaata cgctcagtta ccttcattca
     6901 atctatggac accaccgttg aaacgtagtc tgcttttttc tgccgtgctg tgtgcggcgt
     6961 cattgacctc cgtccatgcg gcgcagccga tcactgaacc ggagtttgcc tctgatattg
     7021 tcgatcgtta tgccgatcat attttttacg gcagcggtgc cacggggatg gcgctggttg
     7081 ttatcgacgg taaccagcgc gtctttcgta gttatggtga aacgcgacct ggtaataacg
     7141 ttcgcccgca gctggattcg gtcgtgcgta ttgcttccct caccaaacta atgaccagtg
     7201 aaatgctggt gaaattgctc gaccagggga ccgtaaagtt aaacgatccg ctaagtaaat
     7261 atgctccgcc aggcgcacgc gtgccgacct acaatgggac gcccattacc ctagtgaatc
     7321 tggcaaccca taccagcgcc ctgccccgtg aacagcccgg tggcgcggca catcgtccgg
     7381 tgtttgtctg gccaacgcgc gagcaacgct ggaaatacct ttctacggcg aagctgaaag
     7441 ccgcgccagg cagtcaggcg gcgtactcta accttgcgtt cgacctgctg gcagatgcac
     7501 tggcgaatgc gtccggcaag ccttataccc agttgtttga agagcagatt acccatccat
     7561 tggggatgaa agacaccacc tacaccccct caccggatca gtgccgccgt ttaatggttg
     7621 ctgagcgcgg tgccagtccg tgcaataaca cgctggcggc aattggcagc ggcggcgtct
     7681 attccacgcc tggcgatatg atgcgctgga tgcagcagta tttgtcatct gatttttatc
     7741 agcgcagtaa ccaggcagat cgcatgcaga cgttgattta tcagcgcgcg cagtttacga
     7801 aagtgattgg catggatgtc cccggcaaag cggatgcgct tggtttaggc tgggtatata
     7861 tggccccgaa agagggtcgt ccgaggatta ttcagaagac aggcggtggt ggcggtttca
     7921 ttacctatat ggcgatgatc ccgcagaaga atattggcgc gtttgtggtg gtcactcgtt
     7981 cgccgttgac gcgctttaaa aatatgagcg atggcatcaa tgatctggtg accgagctaa
     8041 gcgggaataa accgttggtt atccccgcat cctgattaat actctgaagg caaacggttg
     8101 atagcgacca gtttgccttt attcatttcg atatagccgc ctttacgcaa agcagcgaga
     8161 acttctgcga cgacagaacg cgatatgcgt gtacgttgat ggatataatt catcacacca
     8221 atacgtgatc gcaattcttc attccagtca atcattgata ataatgttgc gcgggtttgt
     8281 ttggctggta gatgatatcc cgtacaattt ccttctgata ttaatttgta tggtatatcg
     8341 tttttcatta aaccatcagc cagccccata atataaggag cctgggtaat accgataagt
     8401 acgttttctt ctctacgcag agagataact ccttctaaaa taacgaaagt atcttcgtta
     8461 ttgacatcac tggaaaatat aacttgcttt tcattattaa actcgaagcg cgtaccgtat
     8521 ctggacaaac atttatcgag cttaccaaat tcctgaagag gtttaactac agataacatt
     8581 tgcgcgtcct ttgcagtaat gcccgtcaaa tccttgacgg gcattattta gattaaatta
     8641 ccagtatttc ttcggagtga agaatattac caagtatatt taacacccac gttcgcggac
     8701 cagtcttgat ctacgtcacc accaccgagg tagttagcat cggtataggc gctgaagttc
     8761 ttggtgaagc tgaactgagt acccagacca acacgtaccg cagacccttc agtaccgtta
     8821 tcgatggaat caccgttcac atcgttatcg ttgttagagt cgtcgtagac gtaagccagt
     8881 ttgaagtacg gagtcagagc ctgatcttcg ctgtaggtga aggtataacc tgcatctaca
     8941 cccagttcat aacgcatgct gtcgtaaggc tgaccgtcaa ctttcatgtc gttgctcagc
     9001 tggtagtcat caccagactg gaacagacca gaaatgctgc cgtaaggagt cacgtaacca
     9061 gcatcaccca gtttgaagtc gtaaccggtt ttcaaaccga agccccaagc gtcggagttg
     9121 gtgctaccgt caacgtaagt accgttgctc atggttgcag acaggtcgtt gttgaagtga
     9181 ga
//
LOCUS       DAJTUI010000137         9155 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTUI010000137 DAJTUI010000000
VERSION     DAJTUI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.137"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..913
                     /locus_tag="OO464_003619"
     CDS             <1..913
                     /locus_tag="OO464_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6228623.1"
                     /translation="EAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPPALV
                     LMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQLQF
                     SEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIPFLA
                     AAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVAFIS
                     MALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTTHSF
                     NLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1165..2082
                     /gene="oxyR"
                     /locus_tag="OO464_003620"
     CDS             1165..2082
                     /gene="oxyR"
                     /locus_tag="OO464_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6228624.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2065..3465)
                     /gene="sthA"
                     /locus_tag="OO464_003621"
     CDS             complement(2065..3465)
                     /gene="sthA"
                     /locus_tag="OO464_003621"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6228625.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3786..4436
                     /gene="fabR"
                     /locus_tag="OO464_003622"
     CDS             3786..4436
                     /gene="fabR"
                     /locus_tag="OO464_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6228626.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4436..4795
                     /locus_tag="OO464_003623"
     CDS             4436..4795
                     /locus_tag="OO464_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6228627.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4835..5935)
                     /gene="trmA"
                     /locus_tag="OO464_003624"
     CDS             complement(4835..5935)
                     /gene="trmA"
                     /locus_tag="OO464_003624"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6228628.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6049..6239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6304..8148
                     /gene="btuB"
                     /locus_tag="OO464_003625"
     CDS             6304..8148
                     /gene="btuB"
                     /locus_tag="OO464_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR6228629.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8093..8950
                     /gene="murI"
                     /locus_tag="OO464_003626"
     CDS             8093..8950
                     /gene="murI"
                     /locus_tag="OO464_003626"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6228630.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2091 a         2220 c         2470 g         2374 t
ORIGIN      
        1 cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg cacaaaaaga
       61 acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg ctatcgcgcc
      121 tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg ttatcatcgg
      181 cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc gggcgcagtt
      241 tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc gtccacagct
      301 gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga tgattttagg
      361 tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg gttatttgca
      421 agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc ctttcctcgc
      481 ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga aaaaaggcta
      541 cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt cggcattagg
      601 cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta tctcaatggc
      661 gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg tgatggtgtc
      721 agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg tttttgcttc
      781 ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta atctggcgct
      841 ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta ttgtcaaaga
      901 tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga ataaaaaaaa
      961 gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt tgagaaactc
     1021 tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct gtgagcaatt
     1081 atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat cgccatgaac
     1141 tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct ggtggcattg
     1201 gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca gccgacgctt
     1261 agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga gcggaccagc
     1321 cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg taccgtgctg
     1381 cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat gtccggaccg
     1441 ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat tatcccgatg
     1501 ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac ccaccagtta
     1561 ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt gaaagagagc
     1621 gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat ctatgaagat
     1681 cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga aaaactgctg
     1741 atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt tgaagccggg
     1801 gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa catggtggcg
     1861 gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg caaacgcgat
     1921 ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg cctggtttat
     1981 cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat ccgcgcaaga
     2041 atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta acgcagctac
     2101 ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt actcaatagt
     2161 gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt cggcagcgcg
     2221 ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca aaattttcag
     2281 cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact gggcgcggcc
     2341 cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca cagagctgat
     2401 ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg tggcttcgcc
     2461 ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg ccaggctcgg
     2521 ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat acatgctgtt
     2581 gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg ccagcgaatc
     2641 ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt ttttacccga
     2701 cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact cttcgttgtg
     2761 acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca tctcttgatc
     2821 gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat ccataccgcg
     2881 gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta catggcgcgg
     2941 ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg tgaaatcaac
     3001 atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag cggttagtgt
     3061 ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa agcgagcgtt
     3121 cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc gcgtttgttg
     3181 attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga gcagtcggga
     3241 atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg cgtgacggag
     3301 agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt gataacgctc
     3361 gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt cgccgccggg
     3421 gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag ggcttacctg
     3481 ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc aattgatccc
     3541 cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt acttttgccc
     3601 tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg tttccgctgt
     3661 acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc acaatactgg
     3721 agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc aaggattcag
     3781 acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg ctggtggaag
     3841 ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg cgtgaagtgg
     3901 cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac gtagacgaac
     3961 tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg cgccaggcgc
     4021 gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca tttatggagt
     4081 tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc ggcacctccg
     4141 ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa cttgcggact
     4201 atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc gaagcaatgg
     4261 tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa caacgtcggc
     4321 aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct tattactggt
     4381 atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac gagtaatgaa
     4441 acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt tatcgattaa
     4501 tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc cgattatttc
     4561 cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca tgtcggtagg
     4621 cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca gcacggtagt
     4681 tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct ccgtcattat
     4741 ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg agtaatcggt
     4801 atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt aatacgccgc
     4861 actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg accttgtgcg
     4921 tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag atatacagaa
     4981 tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg cgcggtgggt
     5041 cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg cggttaaact
     5101 cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata atttgtacgt
     5161 tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt ttggcgattt
     5221 cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta ccgttgccgc
     5281 agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc cattccagca
     5341 tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga cgatagatca
     5401 tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc gcgattttgg
     5461 ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt gcatcgcgca
     5521 gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc agggaaacca
     5581 ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg cgcagaacgg
     5641 gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt tcactggcgg
     5701 cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg atgtgataca
     5761 ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga ctgaccggcg
     5821 agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc aaacgtacca
     5881 ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg gtcatgatgt
     5941 atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg tccagatctt
     6001 gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct gtagcatcca
     6061 cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga cgcgcagcgg
     6121 taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga agtcatcatc
     6181 tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt cgcatctggt
     6241 tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg tggatgcttt
     6301 acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt ttccgcttgg
     6361 gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga acagccgcgc
     6421 agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg ctggcagtcg
     6481 acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca aaacggcggt
     6541 tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt gctggtgtta
     6601 attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct tagccagttc
     6661 cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc tgtttatggt
     6721 tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc cggaacggaa
     6781 atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac gcagcaacaa
     6841 ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca tggttatgat
     6901 gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg ttttttaagt
     6961 aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg ctttgttcgc
     7021 ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg ttcaccgttg
     7081 ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta taacggcgaa
     7141 ctgattaaat cacaactcat taccagctat agccatagca aagattacaa ctacgatccc
     7201 cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata caccgtccag
     7261 tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga ctggcagaaa
     7321 cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg taataccggc
     7381 atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc agcacgcagc
     7441 gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg ttgggaattc
     7501 atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact aaatctgggg
     7561 caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa acagtgggaa
     7621 ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata tcgtaacgat
     7681 gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga agggaaagcg
     7741 cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac gcatactgtg
     7801 agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt acgccgtgct
     7861 aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg gggtattact
     7921 tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta tcaaaccgtt
     7981 aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac ctctcacctg
     8041 acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt ctatggctac
     8101 caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac cacgtcccac
     8161 cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga tccggcatct
     8221 cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt atggcgaaaa
     8281 aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc aagaacgtta
     8341 tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac ttcctgcatt
     8401 acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac ctgctgcacg
     8461 tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta aacgttctta
     8521 tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc tgggctcggc
     8581 agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc tggatgcact
     8641 aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg ttgtattggg
     8701 ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag agggaacccg
     8761 gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag aacatgaagc
     8821 cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga cgccagaagc
     8881 tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa aactggcagt
     8941 tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt tcctcttgtc
     9001 aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc aaacacgccg
     9061 ccgggtcagc ggggttctcc tgagaattcc ggcagagaaa gcaaaaataa atgcttgact
     9121 ctgtagcggg aaagcgtatt atgcacaccc cgcgc
//
LOCUS       DAJTUI010000138         9084 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTUI010000138 DAJTUI010000000
VERSION     DAJTUI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.138"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..83
                     /gene="hokE"
                     /locus_tag="OO464_003627"
     CDS             <1..83
                     /gene="hokE"
                     /locus_tag="OO464_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6228631.1"
                     /translation="GDSLCEFTVKERNIEFRAVLAYEPKK"
     gene            434..586
                     /gene="hokE"
                     /locus_tag="OO464_003628"
     CDS             434..586
                     /gene="hokE"
                     /locus_tag="OO464_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6228632.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(708..1328)
                     /gene="entD"
                     /locus_tag="OO464_003629"
     CDS             complement(708..1328)
                     /gene="entD"
                     /locus_tag="OO464_003629"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR6228633.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1503..3743)
                     /gene="fepA"
                     /locus_tag="OO464_003630"
     CDS             complement(1503..3743)
                     /gene="fepA"
                     /locus_tag="OO464_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR6228634.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            3986..5188
                     /gene="fes"
                     /locus_tag="OO464_003631"
     CDS             3986..5188
                     /gene="fes"
                     /locus_tag="OO464_003631"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR6228635.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5191..5409
                     /gene="ybdZ"
                     /locus_tag="OO464_003632"
     CDS             5191..5409
                     /gene="ybdZ"
                     /locus_tag="OO464_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR6228636.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5406..>9084
                     /gene="entF"
                     /locus_tag="OO464_003633"
     CDS             5406..>9084
                     /gene="entF"
                     /locus_tag="OO464_003633"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR6228637.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATL"
BASE COUNT         1971 a         2405 c         2511 g         2197 t
ORIGIN      
        1 ccggagactc gctgtgtgag tttacggtga aggaacgtaa tattgagttc agggctgttc
       61 tcgcttacga accgaagaag tagccgttgt gcggggagta atccccgcat atccggttgt
      121 caggtcagga tggtaaggca cctgctttac actttcgccc gcggtcagtg atggctgcgg
      181 gcgatttcgt tttaagagca atgatgagtt ttgccgacag aagtccgaaa caggactcac
      241 tgtataaata aacagctatt ttgctaagga agggtaagat aacggcgggt gcctgaagct
      301 ttccggtttc aggttaactc tgaggtttgg aaagatgaag ccccaagaga tatttctatc
      361 aatcctgggg ctgccactcc gaacctcaac aactcggatg gtagtccctt cttcgcatgg
      421 aggcaatata aacatgctga cgaaatatgc ccttgtggca gtcatagtgc tgtgtttgac
      481 ggtgctggga tttacgcttc tggtcggaga ctcgctgtgt gagttcaccg taaaggaacg
      541 taatattgag tttaaggctg ttcttgctta cgaaccgaag aagtagccgt tgtgcgggga
      601 gtaatccccg catatccggt tgtcaggtca gagtggtaag gcacctgctt tacactttcg
      661 cccgcagtca gtgatggctg cgggcgaatc gtaccagatg ttgtcaatta atcgtgttgg
      721 cacagagtta tgactatttt ttcttttatc tgccagtgca cagcaaacat ctcattctca
      781 cgatgaatga tgacctgctg tttattccag ctaattatct gatagtccag aaaacctgca
      841 tcagtttgga tctcacttgc cttaaatgcg ctctctttgg cggaaaatgc aagtgtcagc
      901 gccagagaaa aggctaaacc gcagtctgcg agtcgctcgt gttctgctgg tgtaataatg
      961 ttgtctgtca attcggtcgc tgtttgcgca gaaaaaattt cttcgatatc aacgccaatc
     1021 ggttgacggg aaaccacggc caatgccgta gccccgcagt ggctaatact gccgtatacc
     1081 tccgcaggcc acaccggttg gcgtagctcg ccgattgcgg gcacacattt atagccatat
     1141 tcccgcaagg cataaacagc agcgatccgt ccggctaaat gctctgtttt acgtttacgt
     1201 ccagcgtgtt gcagttgtgc gtagtgcggc agccagagta aatcctgctc acaaaaattc
     1261 gccggatcga actcaacaaa atgcagcgta tgtccggcaa aggggaggga ggtatgcgta
     1321 gttttcatat cgaccatatt cgagactgat gacaaacgca aaactgcctg atgcgctacg
     1381 cttatcaggc ctacgtgatc ctgcaatata ttgaattggc aagatctttg taggccggat
     1441 aaggcgttca cgccgcatcc ggcgtgaacg acgcgcactt tgtcaacaat ctgacgttag
     1501 catcagaagt gagtattaat gctcatatac cacgtacgtc ccggctcgtt ataggtatac
     1561 gcgccggcac cggcgatata gttggctcct gccagatcgc ccgtggtctg ggcattacct
     1621 gcacgccaca aacgtttgtc gaacaggttg tccacgccgc cggtcagact gacattcttc
     1681 gtcacatccc aggtcgcgct cagacccaca atgctgtaag gactgatttc tttggtttcc
     1741 gggccaaccg ctggctgacc tttatagttg tacttcttcg gctgctgctt gccgtaccag
     1801 gtgaaggtcg tttgcatcga caaatcttcc cgtgcctgcc agctcagcgt tgagttcaac
     1861 gtgtactccg ggatgatcga caaacggtcg cccgtggttt tgttttcact cttcagcata
     1921 taagtgatgt tattggtcca catcaccgtt tcgctaactg gtacgtttaa cgatccttcc
     1981 agaccttcaa ccactgcttt cggcacgtta tcccactgat agagatcagt gccgactgcg
     2041 ttttgcccta cagccacata gcccgcttca atcttattgc gataatcgtt acggaaccag
     2101 gtgacgcccg ccagccagcc gtcgcgtttg aactccagac caatctcttt gttgatgctg
     2161 gtttctactt tcaggtcatc gttaccttgc agatagcagc cgcccgcgct ggcatagcag
     2221 ccctgacctt tactgtagag aatgtagttc gggttagtct ggtataggct cggcgcttta
     2281 taggcgcggg caatgcctat tttcagcgtg aagtcatcgc ctaaaccttg cgatatgttc
     2341 agcgccgggc tccagttatt gccgacaata ctgtgatgat cgaaacgcag ccccggcgtt
     2401 acgatggtgc tgtcagtcag ctccatgttg ttttcggcaa acagcgagaa aatttctgct
     2461 tttgaatacg ggctacggtc ggtggcactc acgccatcaa tagcgccacc ggtattcgtt
     2521 ccggtcagtg cttgggtgtt ggaacttaag tccttcatcc gttgctgatt ccactccgta
     2581 cccagcgtca gcgtctggtt aacgaggaaa tcaatcggca ggttaacttc gctgtgcagc
     2641 atcacgtcat cgagatcgat atcgacgaaa tcctgtgtcg ctttttcgtt aaatttccct
     2701 tcggtaccgc ccgccagacc ttccggaata cgcgagttac gggtgtgttc atactgcacc
     2761 cagttgctgg tggtcacgcc gttatcccag ccaccgttcc aggtcagcgc gtagttctgg
     2821 cgatacaggc ggttggtttc atcgccatat ttcgagcgag tgtaagcgtc agagttggtg
     2881 ttctgggtat cgcccgcata caggttaccc tggcggctgt aacccgcttc cagttccagc
     2941 gattgcaatg gtgcgaagtc ccagcgcacc acgccgttaa tatctttatt gatcacccct
     3001 tcgcgcccgg ctggtaacgt cgtggcatac gttccggcac gcgcggactg atggccctgg
     3061 ttgatatccc acgcgtcagc ctgagttttg tcgaggttgc catacaagcg gaagctgaat
     3121 tcgtcgccca gcggaccggt caggctaaag ttagtgcgtt tggtggcacc ttcctcttta
     3181 tgttctggcg cattgaaata tgcgtcccag gagccgtgcc actcgccgct gccttttttg
     3241 gtaatgatgt taaccacgcc gcccgccgcg ccgttgccat aacgcgcagc tgccggacca
     3301 cgcagaactt caatacgttc aatcatttca ggtggcaccc aggaggtatc accacgggta
     3361 tcgcgctcgc cacgccagcc ctgacgcacc gagttacggc tgcttaccgg cttgccgtca
     3421 atcaaaatca gcgtgttttc cggacccata ccgcgaatat caatctgtcg gttattccca
     3481 cgctgaccac tggtggagtt accggtcagg ttaacgcctg gcatggtacg gatgatctcc
     3541 gacacatcgc gggcaaccgg gtttttgcgg atttcatctg cggtgatggt cgaaacgcca
     3601 ggagcctgca agttctgctc ggcggcggta acgacaatgg tatcgtcatg tgaaacaggt
     3661 gtatcggtcg gctcttgtgc ctgcgctacc ccataaatcc ccagattgac caacaaggcc
     3721 agggaatgaa tcttcttgtt cattgtttta ttcctgcatt tttgccacga attgcaactg
     3781 tcgggcatgg tcgtcatcaa cacgacgcat cccgttaccg cgaaaacctt tgatcctgaa
     3841 agacacgcag tgcagttggt taattaatgt ccgcgcttcc cacggcgcgc cattacgcta
     3901 ttgcaaatgc aaatagttat taataacatt atcaatatat ttctgcaatc aatgaaaaat
     3961 tgcacagtaa acatggggtt atggtgtgac ggcgttaaaa gtaggaagtg agagctggtg
     4021 gcagtcgaaa catggcccgg aatggcagcg tctgaatgac gaaatgtttg aggtcacttt
     4081 ctggtggcgt gatccccaag gttctgaaga atactcgacg ataaagcgcg tatgggtcta
     4141 catcactggt gtgaccgatc atcatcagaa cagccagccc cagtcgatgc agcgaattgc
     4201 aggtactgat gtctggcagt ggacgacaca actcaatgcc aactggcgcg gcagctactg
     4261 ctttattccc accgaacgcg atgacatttt ttctgcacca tcccccgatc gcctcgaatt
     4321 acgtgaaggc tggcgaaaac tattacccca ggcaatagcc gatccgctga atccacaaag
     4381 ctggaaaggc gggctagggc acgctgtttc tgcactcgaa atgccgcaag cgcctctgca
     4441 accgggatgg gattgtccgc aagcgccaga aataccagcc aaagaaatta tctggaaaag
     4501 tgaacggttg aaaaattcac gtcgtgtatg gatttttacc accggcgatg taacagcaga
     4561 agaacgcccg ctggcagttt tgctcgatgg cgaattttgg gcgcaaagta tgcccgtctg
     4621 gcctgtgctg acttcgctga cccatcgtca gcaacttcct cccgccgtgt atgtgttgat
     4681 cgacgctatc gacaccacgc accgcgccca cgaactgccg tgtaatgcgg atttctggct
     4741 ggcagtacag caagagttat tacccctggt gaaagttatt gcccctttta gcgatcgcgc
     4801 cgatcgcacc gtggtcgccg ggcagagttt tggtgggctg tccgcgctgt atgccggact
     4861 gcactggcct gaacgctttg gctgtgtatt aagccagtca ggatcgtact ggtggccgca
     4921 tcggggcggg cagcaagagg gcgtgttact tgaaaagcta aaagctggtg aagttagcgc
     4981 cgaaggtctg cgcattgtgc tggaagcggg tattcgcgag ccgatgatca tgcgggccaa
     5041 tcaggcgctg tatgcgcaat tacaccctat aaaagaatcc attttctggc gtcaggttga
     5101 cggcggacat gatgcgcttt gttggcgcgg tggcttgatg caggggttaa tcgacctctg
     5161 gcaaccactt ttccatgaca ggagttgaat atggcattca gtaatccctt cgatgatcca
     5221 cagggagcgt tttacatttt gcgcaatgcg caggggcaat tcagtctgtg gccgcaacaa
     5281 tgcgccttac cggcaggctg ggacattgtg tgtcagccgc agtcacaaga gtcctgccag
     5341 cagtggcttg aagcccactg gcgtactctg acaccggcga atttcaccca gttgcaggag
     5401 gcacaatgag ccagcattta cctttggtcg ccgcacagcc cggcatctgg atggcagaaa
     5461 aactgtcgga tttaccctcc gcctggagcg tggcgcatta cgttgagtta accggagagg
     5521 ttgatgcgcc attactggcc cgcgcggtgg ttgccggatt agcgcaagca gatactttgc
     5581 ggatgcgttt tacggaagat aacggcgaag tctggcaatg ggtcgatgat gcgctgatat
     5641 tcgaactgcc agaaattatc gacctgcgaa ccaatattga tccgcacggt actgcgcagg
     5701 cattaatgct ggcggatttg caacaagatt tgcgcgtcga tagcggtaaa ccactggtct
     5761 ttcatcaact gatacaggtg gcagataacc gctggtactg gtatcagcgt tatcaccatt
     5821 tactggtcga tggcttcagt tttccggcca ttacccgcca gatcgccaac atttactgcg
     5881 cattgctgcg tggcgaacca acgcctgctt cgccgtttac gcctttcgct gatgtagtgg
     5941 aagagtacca gcaataccgc gaaagcgaag cctggcagcg tgatgcggca ttctgggtgg
     6001 aacagcgtcg ccaactgcca ccgcccgcgt cactttctcc ggcaccttta gcggggcgca
     6061 gcgcctcggc agatattttg cgtctgaaaa tggaatttac cgacggggaa ttccgccagc
     6121 tggctacgca actttcaggt gtgcagcgta ccgatttagc ccttgcgctg acggctttgt
     6181 ggctggggcg attgtgcaat cgcatggact acgccgccgg atttatcttt atgcgtcgac
     6241 tgggctcggc ggcgctgacg gctaccggac ccgtgctcaa cgttttgccg ctgggtattc
     6301 atattgcggc gcaagaaacg ctgccggaac tggcaacccg actggcaacc cgactggcag
     6361 cacaactgaa aaaaatgcgt cgtcatcaac gttacgatgc cgaacaaatt gtccgtgaca
     6421 gcgggcgagc ggcaggtgat gaaccgctgt ttggtccggt actcaatatt aaggtatttg
     6481 attaccaact ggatattcct ggtgttcagg cgcaaaccca taccctggca accggtccgg
     6541 ttaatgacct tgaactggcc ctgttcccgg atgaacacgg tgatttgagt attgagatcc
     6601 tcgccaataa acagcgttac gatgagccaa cgttaatcca gcatgctgaa cgcctgaaaa
     6661 tgctgattgc ccagttcgcc gcggatccgg cgctgttgtg cggcgatgtc gatattatgc
     6721 tgccaggtga gtatgcgcag ctggcgcaga tcaacgccac tcaggttgag attccagaaa
     6781 ccacgctgag cgcgctggtg gcagaacaag cggcaaaaac accggatgct ccggcgctgg
     6841 cagatgcgcg ttacctgttc agctatcggg aaatgcacga gcaggtggtg gcgctggcga
     6901 atctgctgcg tgagcgcggc gttaaaccag gggacagcgt ggcggtggca ctaccgcgct
     6961 cggtcttttt gaccctggca ctccatgcaa tagttgaagc tggcgcggcc tggctaccgc
     7021 tggataccgg ctatccggac gatcgcctga aaatgatgct ggaagatgcg cgtccgtcgc
     7081 tgttaattac caccgacgat caactgccgc gctttagcga tgttcccaat ttaaccaacc
     7141 tttgctataa cgccccgctt accccccagg gcagtgcgcc gctgcaactt tcacaaccgc
     7201 accacacggc ttatatcatc tttacctctg gctccaccgg caggccgaaa ggggtaatgg
     7261 tcgggcagac ggctatcgtc aaccgcctgt tgtggattca aaatcattat ccgctcacag
     7321 gcgaagatgt cgttgcccaa aaaacgccgt gcagttttga tgtctcggtg tgggagtttt
     7381 tctggccgtt tattgccggg gctaaactgg tgatggctga accggaagcg caccgcgacc
     7441 cgctcgctat gcagcaattc tttgccgaat atggcgtaac gaccacgcac tttgtgccgt
     7501 cgatgctggc agcatttgtt gcctcgctga ctccgcaaac cgctcgccag agttgcgtga
     7561 cgttgaaaca ggttttctgt agtggtgagg ccttaccggc tgatttatgc cgcgaatggc
     7621 aacagttaac cggcgcgccg ttgcataacc tgtatggccc gacggaagcg gcggtagatg
     7681 tcagctggta tccggctttt ggcgaggaac tggcacaggt gcgcggcagc agtgtgccga
     7741 ttggttatcc ggtatggaat acgggtctgc gtattcttga tgcgatgatg catccggtgc
     7801 cgccgggtgt ggcgggtgat ctctatctca ccggtattca actggcgcag gggtatcttg
     7861 gacgacccga tctgaccgcc agccgcttta ttgccgatcc ttttgctcct ggtgaacgga
     7921 tgtatcgtac cggagacgtt gcccgctggc tggataacgg cgcggtggag tacctcgggc
     7981 gcagtgacga tcagctaaaa attcgcgggc agcgtatcga actgggcgaa atcgatcgcg
     8041 tgatgcaggc gctgccggat gtcaaacaag ccgttaccca cgcctgtgtg attaaccagg
     8101 cggcagccac tggtggtgat gcgcgtcagt tggtgggcta tctggtatcg caatcgggcc
     8161 tgccgttgga taccagcgca ttgcaggcgc agcttcgcga aacattgccg ccgcatatgg
     8221 tgccagtcgt tctgctgcaa ctgccgcagt tgccacttag cgccaacggc aagctggatc
     8281 gcaaagcctt accgttgcct gaactgaagg cacaagcgcc agggcgtgcg ccgaaagcgg
     8341 gcagtgaaac gattatcgcc gcggcattcg cgtcgttgct gggttgtgac gtgcaggatg
     8401 ccgatgctga tttctttgcg ctgggcggtc attcgctact ggcaatgaaa ctggcagcgc
     8461 agttaagtcg acagtttgct cgtcaggtga cgccggggca ggtgatggtc gcgtcaaccg
     8521 tcgccaaact ggcaacgatt attgatggtg aagaggacag ctcccggcgc atgggattcg
     8581 aaaccattct gccgttgcgt gaaggtaatg gcccgacgct gttttgtttc catccggcgt
     8641 ccggttttgc ctggcagttt agcgtgctct cgcgttatct cgatccacaa tggtcgatta
     8701 ttggcattca gtcgccgcgc cctcatggac ccatgcagac agcgacaaac ctggatgaag
     8761 tctgcgaagc gcatctggca acgctacttg agcaacaacc gcacggcatt gcggcgcggc
     8821 tgcgtgcccg tggcgaacag gtggcatttc ttggtttgct ggatacctgg ccgccagaga
     8881 cgcaaaactg gcaggaaaaa gaggctaatg gtctggaccc ggaagtgctg gcggagatta
     8941 accgcgaacg cgaggccttc ctggcggcac agcagggaag tacttcaacg gagttgttta
     9001 ccaccattga aggcaactac gctgatgctg tgcgcctgct gacgactgct catagcgtac
     9061 cgtttgatgg taaagcgacg ctgt
//
LOCUS       DAJTUI010000139         9019 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTUI010000139 DAJTUI010000000
VERSION     DAJTUI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.139"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(87..1463)
                     /gene="trkA"
                     /locus_tag="OO464_003634"
     CDS             complement(87..1463)
                     /gene="trkA"
                     /locus_tag="OO464_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6228638.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1485..2774)
                     /gene="rsmB"
                     /locus_tag="OO464_003635"
     CDS             complement(1485..2774)
                     /gene="rsmB"
                     /locus_tag="OO464_003635"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6228639.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2820..3767)
                     /gene="fmt"
                     /locus_tag="OO464_003636"
     CDS             complement(2820..3767)
                     /gene="fmt"
                     /locus_tag="OO464_003636"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6228640.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3782..4291)
                     /gene="def"
                     /locus_tag="OO464_003637"
     CDS             complement(3782..4291)
                     /gene="def"
                     /locus_tag="OO464_003637"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6228641.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4456..5580
                     /gene="dprA"
                     /locus_tag="OO464_003638"
     CDS             4456..5580
                     /gene="dprA"
                     /locus_tag="OO464_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6228642.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5552..6025
                     /locus_tag="OO464_003639"
     CDS             5552..6025
                     /locus_tag="OO464_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6228643.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6054..6596
                     /locus_tag="OO464_003640"
     CDS             6054..6596
                     /locus_tag="OO464_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR6228644.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6601..7173
                     /gene="tsaC"
                     /locus_tag="OO464_003641"
     CDS             6601..7173
                     /gene="tsaC"
                     /locus_tag="OO464_003641"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6228645.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7178..7996
                     /gene="aroE"
                     /locus_tag="OO464_003642"
     CDS             7178..7996
                     /gene="aroE"
                     /locus_tag="OO464_003642"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6228646.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7993..8250
                     /locus_tag="OO464_003643"
     CDS             7993..8250
                     /locus_tag="OO464_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6228647.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8226..8780)
                     /locus_tag="OO464_003644"
     CDS             complement(8226..8780)
                     /locus_tag="OO464_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6228648.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2254 a         2190 c         2251 g         2324 t
ORIGIN      
        1 ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa agggaaataa
       61 taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg gaagagtctt
      121 tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc gccttgctca
      181 atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc tccaataatc
      241 gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga ggtgctttca
      301 tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga ggaaacacca
      361 acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg ttgtggtgaa
      421 attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg ctggatcaat
      481 accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat attggcctcg
      541 tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc ggccagcagt
      601 tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc cgccagttcg
      661 gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc cagacgacgc
      721 gccagccctg caccgatatt accgccacca accagcatga tccgtttata cggtttttcc
      781 agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat aaagaacact
      841 tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc gtggcggaaa
      901 attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga aagtgcatta
      961 ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt accctcagcg
     1021 aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt atcgataacc
     1081 aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa tagcttatcg
     1141 gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt gttgaaaagt
     1201 gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac agcaaccagc
     1261 atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc atgcccctgc
     1321 acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt ggtatcgaca
     1381 acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc aacctggccg
     1441 gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt tttgattagc
     1501 ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt acccggttgc
     1561 tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa ggctttaatc
     1621 tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt tccaccggat
     1681 tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag ttccgggata
     1741 tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc cgttgctgaa
     1801 caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg ggaagggtaa
     1861 cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt gtcgtaaatg
     1921 cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg tgccacctca
     1981 aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg ttcaccgttt
     2041 tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt aacccatccg
     2101 tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac agcatcaggg
     2161 taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag ccagctgtcg
     2221 cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt attggcttcg
     2281 gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa ccaggaagga
     2341 tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg ccgacgctgg
     2401 aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat tgcgatagcg
     2461 ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag cagttgatac
     2521 aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg acgggccatt
     2581 aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa gcacaactct
     2641 tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag aatgttgctt
     2701 aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct acgtaaatta
     2761 cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag agtggactat
     2821 cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag gtcttgcgcg
     2881 ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc atcaccagtc
     2941 gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc gttggttgcc
     3001 gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc cagccagctc
     3061 attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga aagtgaccag
     3121 tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt ttcgtcctga
     3181 acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag cccttgtggg
     3241 ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt aatcgggcag
     3301 gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat aatggttaca
     3361 ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc gcgccagcgt
     3421 ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag cacagcttta
     3481 ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc ggcgaccagt
     3541 tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc ttcttcctca
     3601 gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg tcggtctggc
     3661 tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag atgacgcgct
     3721 gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt agttcttatc
     3781 cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt cgttgttgtt
     3841 tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc tcatgctgaa
     3901 tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg cggtcaaggg
     3961 cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg atcgacaggc
     4021 aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg attaacacca
     4081 gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg atatcaacct
     4141 gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata tcatcgacga
     4201 tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga agccgctcgt
     4261 ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt caggagttag
     4321 aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc acttgattga
     4381 cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag gcttgcttat
     4441 ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag catcagcagc
     4501 ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc gcatattgat
     4561 gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt tctttcattt
     4621 ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca tcatttaatc
     4681 cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta ccccggcgca
     4741 ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt agtggggagt
     4801 cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac tctggcgacg
     4861 tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt gcataaagcg
     4921 gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa taccattcat
     4981 ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc tctcgtctcg
     5041 gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa tcgcattatc
     5101 agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg ttcgctggtg
     5161 acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg tccaataggg
     5221 aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct tgtgacggaa
     5281 ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga cgcccctgaa
     5341 aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga gctcctggct
     5401 aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg ccaacctgtg
     5461 ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc agctgtaccc
     5521 ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt atttgtttga
     5581 aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg aacaggatct
     5641 taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc tggaaaaact
     5701 tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc ctctctccat
     5761 gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg ggtttctgct
     5821 tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga tagagcgagt
     5881 gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga tcctgatggt
     5941 gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat tactctttga
     6001 agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt gttatggcaa
     6061 aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg gctgaactgg
     6121 ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg gcgtgtgact
     6181 acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg gaggggcagg
     6241 tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt atgtttattg
     6301 gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg gacgaaacag
     6361 caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc cgttatggca
     6421 aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac ttcaaaccaa
     6481 tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa accgcgcagg
     6541 gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg gaataataac
     6601 gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct caatgaaaaa
     6661 cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc tgatagcgaa
     6721 acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg gctgatttta
     6781 atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt gactgacgtg
     6841 cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt tcccgcgcct
     6901 gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg agtcaccgac
     6961 catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc taccagtgcc
     7021 aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca atttggcgcg
     7081 gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga aatccgcgat
     7141 gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg ctgtttttgg
     7201 taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc agcaactgaa
     7261 tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca acacactgaa
     7321 cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta aagaagaggc
     7381 ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag ttaataccct
     7441 caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag gcttgttaag
     7501 cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta tcggcgctgg
     7561 tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg tgacaatcac
     7621 taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca ctggcagtat
     7681 tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta ttaatgcaac
     7741 atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc atccaggcat
     7801 ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat ggtgtgagca
     7861 gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg cggctcatgc
     7921 ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc aattgcagga
     7981 ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg ggacgagaat
     8041 aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg cgcgatctct
     8101 ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt agcgagtttt
     8161 cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga acaaagtgaa
     8221 gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca tttcacgtaa
     8281 ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg gatctgtttg
     8341 acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg gaccagacta
     8401 cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat tgagcccatc
     8461 ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg accaacagtg
     8521 acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt atgagtgaca
     8581 tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata atgtacatct
     8641 ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac gtcaccaatc
     8701 acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag atcgcggtat
     8761 gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat gactttgggg
     8821 gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt gtgtgcgatt
     8881 gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat acttgtgcaa
     8941 aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca cttcacagga
     9001 tggtcggaac aacgaagag
//
LOCUS       DAJTUI010000140         8754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTUI010000140 DAJTUI010000000
VERSION     DAJTUI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.140"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..428
                     /locus_tag="OO464_003645"
     CDS             <1..428
                     /locus_tag="OO464_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR6228649.1"
                     /translation="LNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFAVQ
                     SGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKL
                     NVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(575..2737)
                     /gene="uvrD"
                     /locus_tag="OO464_003646"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(575..2737)
                     /gene="uvrD"
                     /locus_tag="OO464_003646"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6228650.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2821..3537)
                     /gene="yigB"
                     /locus_tag="OO464_003647"
     CDS             complement(2821..3537)
                     /gene="yigB"
                     /locus_tag="OO464_003647"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6228651.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3537..4433)
                     /gene="xerC"
                     /locus_tag="OO464_003648"
     CDS             complement(3537..4433)
                     /gene="xerC"
                     /locus_tag="OO464_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6228652.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4430..5137)
                     /locus_tag="OO464_003649"
     CDS             complement(4430..5137)
                     /locus_tag="OO464_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6228653.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5134..5958)
                     /gene="dapF"
                     /locus_tag="OO464_003650"
     CDS             complement(5134..5958)
                     /gene="dapF"
                     /locus_tag="OO464_003650"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6228654.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(5995..6198)
                     /locus_tag="OO464_003651"
     CDS             complement(5995..6198)
                     /locus_tag="OO464_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6228655.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6387..7865
                     /locus_tag="OO464_003652"
                     /pseudo
     CDS             6387..7865
                     /locus_tag="OO464_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7862..>8754
                     /locus_tag="OO464_003653"
     CDS             7862..>8754
                     /locus_tag="OO464_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6228656.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPE"
BASE COUNT         2032 a         2388 c         2246 g         2088 t
ORIGIN      
        1 cgttaaatct cgcttcaggt gaagggaatt tctttatccg tcctggcggc gtgttttatg
       61 tcgcgggaga taaagtcggc atcgttcgtc tggatgcttt taaagccagt aaagatattc
      121 agtttgcggt gcagtcagga ccaatgttga tggaaaacgg tgtaattaat ccgcgtattc
      181 atcccaacgt cgcctcacgt aaaattcgta acggtgttgg gattaataaa catgggaatg
      241 ccgtgttttt gttgagccag caggcgacaa atttttatga ttttgcctgt tatgccaaag
      301 cgaaactgaa cgttgagcag ctgctttatc ttggtggcac gatttcacat atgtatatga
      361 aaggcggggc aatcccgtgg caacgctatc cttttgtcac catgatttcc gtcgagcgga
      421 aaggttagaa tgatagcttt gtgtcagata ctaaatatgc tgctgatgcc ggatgcggcg
      481 taaacgcctt atccggccta catgacgttg caatttattg aatctgaatg attttttagg
      541 ccaaataagg tgcgcagcac cgcatccggc aacgttacac cgactccagc cgggcgtaag
      601 ctgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
      661 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgt acacgctggc
      721 cgagcttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctgaccg
      781 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc aactcgccaa
      841 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
      901 tcagcgtcag tttctgcatc gcgcgggtta cgccaacgta ggccagacgg cgttcttctt
      961 ccagacgccc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1021 cgataaacac ctgcgggaac tccagacctt tcgccgagtg catggtcatc agttgtaccg
     1081 catcctgcca ggtatccgcc tgcccttcgc ctgcttccag cgccgcatgg gataggaacg
     1141 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     1201 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     1261 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     1321 cagtttcctg cgctaaggcg tcgatcagtt ccataaaccg ctgtaaggcg ctggcagcac
     1381 gtccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     1441 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     1501 tattcaccac acgctcaaag gccgcgtcgt cgttacggtt ggcaatcagg cgcagatacg
     1561 agagcgcatc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     1621 gcatactcgc ctgcaacagt gcttcttcca gcacacgcga ctgggcgtta ctgcggtaga
     1681 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg cggttaacca
     1741 caaaacgcgc ttcatcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     1801 cgccatcggt ccacagtttt ttacccagac gcccgttatt gttttcaatc agggcgttag
     1861 cggcgctcag aatattgctg gtagagcggt agttttgctc cagacgaata atttcggcac
     1921 cggggaaatc attaaggaaa cgctgaatat tctccacctg cgccccgcgc cagccgtaga
     1981 ttgactggtc gtcatcaccg acgatcatca ctttgccggt gtcgcccgcc agcaggcgga
     2041 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggata ttggtaaaac
     2101 gttcgcggta gtgttgtagg atatgcggct tgttaagcca cagttcgtga gcgcgcagca
     2161 gcagctcggc gaagtccacc aggcccgcgc gatcgcacgc ttcctgatac gcctgataca
     2221 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     2281 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     2341 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttcg ctatcgagga
     2401 tctggaaatc ctgcggcaga ttggcgtcca tatggtgcgc acgcagcagg cggtgcgcca
     2461 gcccgtggaa ggtgcctacc cacatgccgc cctggctggt gcccatcagt tgtccgatac
     2521 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     2581 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     2641 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct tcgcgttgtt
     2701 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     2761 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttgctga
     2821 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgtggca gtaaacggct
     2881 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     2941 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3001 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3061 gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     3121 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     3181 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     3241 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     3301 cgaacgaaaa cgccaacgtg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     3361 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     3421 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     3481 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     3541 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     3601 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagtagctc ctgcacacca
     3661 cgaagatcgc cgctcgactc cagcatatgc gtagcgaacg agtgacgtaa tttatgtggg
     3721 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     3781 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     3841 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     3901 cggcgctctt tgctgccttt ccccatcacc cacacttcgc cggactccag gtcgaggtgt
     3961 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4021 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tatccagcag ccgattcata
     4081 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aactcctttc
     4141 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     4201 gaaagccgta acgccagact tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     4261 ttgcgtacca tcgctgcatc gcattgttgc cagctttgca ggccgttttc gctggcaaaa
     4321 ttgatgatcg cctcaagctg acgctggtag ttaagtaggg ttatcgggct aagctgacgc
     4381 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     4441 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     4501 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacaaca cccaaatcag
     4561 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     4621 ccagcagctc cggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     4681 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     4741 ctaagcgcca gcgatctgga aacaggcgca gactcgcacc agctaggccg agatcgcgcg
     4801 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     4861 gcaggtagag taggcgataa aacagccctt cgttggcgat agcctgctcc atcaacaacg
     4921 ccatgttctc ttccagaaca tgaatgtgat tacgtgcgcg ggccatgtgc cactcgacca
     4981 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattgc
     5041 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     5101 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc catcgtagac
     5161 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     5221 acgaccgccg gggagttcca cgcgtacttc ttcgtccagc aaaccttgct gaatccctac
     5281 cgccaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     5341 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     5401 cggaaaacgc tcgtgacttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     5461 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     5521 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     5581 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     5641 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     5701 cagacgcaca aaacgggcaa agcagcgcgc accgttaccg cactgcgcca cttcactgcc
     5761 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     5821 cagcagctgg tcaaacccta cccccaggtg ccgatccgcc aggcgacgaa tcagctccgg
     5881 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     5941 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6001 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6061 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     6121 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     6181 cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg ctttctatca
     6241 tcgcaggaga aggcgtaaaa gcaagcgttt agcgactctt tatgaagtct gaaaagtgag
     6301 gaaatgctgc gagtataacg cttactcgtg aggatttatg ttctgctggc agtttcgtga
     6361 tcaacacctc gcgccaggat ggctttatgc actatcgcaa tgtggtttgt ttgttgtctt
     6421 gttcattgtt tttatcgtcg gcatggggat gtcggctttc cgcacctgaa cataatattt
     6481 accagaaaca gggaaaaggc gtggtgtacc tgcgccctta taaagaaaca aatctttctc
     6541 tacctgacgt gaattacaag agtttgcgtc ggttacccaa tttattaata gacccgacaa
     6601 cactagacga atgggataaa gaaccgcctc tgactgattt gacaaccgac tacctctatg
     6661 agggcgcaca ggcctggtat cctcattatt catggcatag cgatggtcgc aatatcctct
     6721 acgcgggtga agttgtgcaa aaccctcccg gcacacctcc cgtcgatgtc gcctcgttca
     6781 aggcatgggg tgattttgcc gcagataaac acagtctcta ttttgaaggc aagcgcaccg
     6841 atgacaatgg tggtggaaat agcttggata tcaaaacgct gcatcaggtg gaattccggc
     6901 ctccctggga tcctgactta ctggggctga tattacgtga cgctaatttc ttgtatatca
     6961 atgggcatcg cctcgctgac cccgagagtt tccgtgtgct ggcgcagaag tcctgggatc
     7021 agcgaggaaa gttttccacc acgtttaacc cctgcattgc cgtaccattt ggcccctggg
     7081 ataccctggc tcgtacacgg accaaaatcc tgctcaacgg cgaacagctt gatgccgacc
     7141 cggacacctt ttccgtcgtg cgctggatgc ccggctcact cttgacctgg cgtgataaaa
     7201 acgggctgca gcgtaaagtc ctcgacaagg aaaatctggc gtgggatgaa gatttaacaa
     7261 agcactgtct ggatttttct ctgctggaaa agaaagtgtt ctggcgtaaa ggacctgctt
     7321 gtaaacagga agaattaccc ggactcgatc cggagcagtt tcaacccatc agtgatgctg
     7381 tcgcccagca tcaggactcg ctttatacca tcatcgaaac agagtctggt aaccgtaagc
     7441 tggagatcgt gaaacttgat gatcccaatc ttattatcaa caaacgtttc aacgccggga
     7501 aacgccacgg ctatttactt acgcgtgccg aagggtagcc ataccattcc ggtttacacg
     7561 tgtttgaatc tgacggaccg ctgatcttac tggataaccg ttctccggat gaacgcgaag
     7621 cccatcttaa tgaccatccc tttttgcgca gatggtatgc ccgcgataac cgctacattt
     7681 acagctttga tggcgcgcag ctctggcgat accgcaccgc tgatccgaaa caagttcgct
     7741 taatctggaa ggaacaacat tcgggatatg tctatggcgt aaattacaaa acgggatatc
     7801 tggacggaaa aattactgat gacggcgaat ttattcctgc cccgcgcaat gaggcgacaa
     7861 aatgaagggt caaataatca cagttgcact catcctgttg ggacacacat ttctgacgcc
     7921 tgctgtacag gccatagggt tcgactatta caatgatcac ggtgttatgt cttacggtaa
     7981 aggttattgg ggggataaga aaatcattgc ccagtttccg gagatgagca gggccggttt
     8041 gcgccttgtg aaaaatatct ctgggaaaac gggttatata ccttccgatg aaataagcat
     8101 agaaaatgag gaataccact gggtgacaga cggccgcgtt attctctggc gtggcaaaat
     8161 cgttagcaat ccaccgggaa cactcactgt cgatattgcc agctttcagg ctatgggccg
     8221 cttcgcggtc gataaatata gcctctattt tgacggacag cgcaccgaaa gtaatagcgg
     8281 cgcgtcacgt gttgagctgg cgacactaaa agctatcgaa ggtaactcta ccacgctggt
     8341 ggatagtaaa aatctctact tgtccggtcg acgtcagggg agcagcagtg atgttactgt
     8401 attagaaaaa agatggtggg gtattaatcc acgtcttatg agtgtaaaca gaaattcata
     8461 ttccaatgat ctgcttatcc gcagtgggca gaatatttat ttaaatggcg ttcaccttac
     8521 ggcgaatgca gactcatttg agataattcg ctggatacct cattcactgc tggtttttcg
     8581 cgacaataag ggtctgcatc gttatccctt tggtcaatta tcaggcaaag cgatacccgt
     8641 agatgatgac gtctcttttg aagtagggga aagtcgcgtt cgctggcgta aacagctcac
     8701 gcccgaccgt cagtggagca agtggataga cctaccaggt attgaacctg aaca
//
LOCUS       DAJTUI010000141         8644 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTUI010000141 DAJTUI010000000
VERSION     DAJTUI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.141"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(766..1962)
                     /gene="hemY"
                     /locus_tag="OO464_003654"
     CDS             complement(766..1962)
                     /gene="hemY"
                     /locus_tag="OO464_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6228657.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1965..3182)
                     /gene="hemX"
                     /locus_tag="OO464_003655"
     CDS             complement(1965..3182)
                     /gene="hemX"
                     /locus_tag="OO464_003655"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6228658.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQGDTPAAAPQGE"
     gene            complement(3204..3944)
                     /gene="hemD"
                     /locus_tag="OO464_003656"
     CDS             complement(3204..3944)
                     /gene="hemD"
                     /locus_tag="OO464_003656"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6228659.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3941..4882)
                     /gene="hemC"
                     /locus_tag="OO464_003657"
     CDS             complement(3941..4882)
                     /gene="hemC"
                     /locus_tag="OO464_003657"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6228660.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5269..7815
                     /gene="cyaA"
                     /locus_tag="OO464_003658"
     CDS             5269..7815
                     /gene="cyaA"
                     /locus_tag="OO464_003658"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6228661.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7855..8175)
                     /gene="cyaY"
                     /locus_tag="OO464_003659"
     CDS             complement(7855..8175)
                     /gene="cyaY"
                     /locus_tag="OO464_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6228662.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8223..>8644)
                     /locus_tag="OO464_003660"
     CDS             complement(8223..>8644)
                     /locus_tag="OO464_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6228663.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1967 a         2235 c         2225 g         2217 t
ORIGIN      
        1 tgacggggta tctgctgcgc tggccatgag aggaagagcg gcggcgacag caacaacaag
       61 acgtttgggt gaaaacgaaa attccatgca aaatgctccg gtttcatgtc atcaaaatga
      121 tgacgtaatt aagcattgat aattgagatc cctctccctg acaggatgat tacataaata
      181 atagtgacaa aaataaatta tttatttatc cagaaaatga attggaaaat caggagagcg
      241 ttttcaatcc tacctctggc gcagttgata tgtaaggcag gtttattata tcgcgttgat
      301 tattgatgct gtttttagtt ttaacggcaa ttaatatatg tgttattaat tgaatgaatt
      361 ttatcattca taataagtat gtgtaggatc aagctcaggt taaatattca ctcaggaagt
      421 tattactcag gaagcaaaga ggattacaga attatctcat aacaagtgtt aagggatgtt
      481 atttcccgat tctctgtggc ataataaacg agtagatgct cattccatct cttatgttcg
      541 ccttagtgcc tcataaactc cggaatgacg cagagccgtt tacggtgctt atcgtccact
      601 gacagatgtc gcttatgcct catcagacac catggacaca acgttgagtg aagcacccac
      661 ttgttgtcat acagacctgt tttaacgcct gctccgtaat aagagcaggc gtttttttat
      721 gcatcagaaa ggtcccggag gtgctttcct ccgggtgaga aggaactact gtggcgggtt
      781 attctgcaac gttaacatca aaccgtcgcg acgcatcgct gcagcttctt ccggcttgtg
      841 cagtctgtcc agcgcgtcgg caagccatgc gtaatcgtag gcgtccggac gttgtttcag
      901 cgccgcgcgg aaggcgagcg atgcttcctg ccattcaccg tgcttcatca gtgactgacc
      961 caatgtgctc cacaacagcg ggcgatcacc gacgtttttg atttgctggc gtagcacttt
     1021 ttccagctgt tccgggttat tggttttcag acgcgggatc ggcagcagta ggcgatcgtc
     1081 atactggcgt ttcaggccgt cgatgataat ttgctgggca gtatcatgat cgtcacattc
     1141 aataagatgt tccgccattg ccacctgcaa cgctacctga tgacgcgttt tccggctttg
     1201 gtttttccac cagttacgca aaccttcact accgttatcg gcacgcgcct gatccatcag
     1261 gccaatccat gcctgttgtt ccagcattgc acgatgttct tcatcaccaa catgggcttt
     1321 cgccattgat gggatgatat ccagcagaga actccatgcc cctgtgcgga tatacgcctg
     1381 ttccgccaga cgtaatactt caggatggcg tggcgtaact tccagcagct tatccacgcc
     1441 gtggcgtgca gcatggtttt cattacgggc cagttgcaga cgtacacggg tgatttctac
     1501 cggaatggtg tcattgccgg ccagctccgc tgcgcgttcc agatgttggt tggcgcgtgc
     1561 ttcatcacca cgttgttgcg cggcttcggc agccagtaga tagttcacca ccggttgttc
     1621 agcgtgatcg gcatttttcg ccatcagctt ttcaacttgc tgataatcac cttccgccag
     1681 tttcagcagc gcctgttcgg tctgcttacg cgcacggcga cgcttacgtc cgacaaacca
     1741 tccacgggtg tgcgcgccag tgcggaagat ccgccgcagt agccactcaa tggcaaacag
     1801 cactaccatc gccagaatca atatgatcgc caggcccgtg acgctggttt cgatattgta
     1861 gttgtcggtc tggatcagca cataaccctg atgaccggca atcatcgggc caaccactat
     1921 ccccgcaatc agcaaaacaa agagtaataa cacttttagc atggttattc tccttgcggc
     1981 gcggctgccg gagtatcacc ttgcggtgca ggtgcaggtg caggtgcagg tgcaggtgca
     2041 ggtgcaggtg caggtttagc ttccgttgtc cccgctgccg gttgtgccag caggttacgc
     2101 acgcgagtct gcatcagttt ttccagcatc gcctggcttt gcagggtttc cggaagatcc
     2161 atcgagatat tttgctggct taactggtcc acctcgtcga ggaacgcttt ggtggtggca
     2221 tcatcagtat cgtagtaagc acgtacccag gtggagacgt tctccagcgc ctggcgataa
     2281 gtctcttcct gatgacgcgg tacagcttgt gctgcgacca gcaggcgaga gcgaatattt
     2341 tcgcgcagat agatatcctg atttggcgct aacagcggta cggcggtgtc atcacgacgg
     2401 cgaatcgtaa tgaagttgtc cataaagttc tgccagcttt tttgcagatt gatacgccat
     2461 tcgctgatgg aactggaaag ctcttcaccg tctgaatcca tcggcgaacc atcgctgtca
     2521 ttatcggcca gacgcaggtt atctacctga tttgaaagct gattaagctt aaggatgatg
     2581 ccgtcataat ccacctgcga tactgcagaa aggctggcga tatcatcggt aattgcccga
     2641 cgaacggtaa tcagactcgg gtcattcata tccgccaggc tggcgtctgc acttttcagc
     2701 aacgctgcag aggtcgtgac gtcctgatcg ctccacagct tccgtccggc gagtttcacc
     2761 agaaaatcgg cctgagccag cagccaggtt ttagcatcgc tgccggaaat ggtggcgatc
     2821 ttttgttgga cttcatccaa ctgttttgcc agcgtttctt gctgacgatt cgcctgctta
     2881 agctgcgcag cttgttgctt aataatgcct tccagctcgg ctttttggct ctcctgggct
     2941 ttttgcaatg ccgtcagttg gttagccagg gcatcgctgg tggcggtctg attgacggcc
     3001 tgttgtttac cccagccata caaaccgaca cccgccgcca gagcaatagc gatagccacc
     3061 gcgctgagaa tcaatgcagt attgttctta ctcttttttt ctgttgcgac aggttgtgac
     3121 gtggtgtcca cggcctccct ggtctcttca accacggcgg aggttttttc ttgttccgtc
     3181 attatggctt cctgttatga gagttattgt aatgcccgta aaagcgcatc gttgtcagcg
     3241 ttatcggcga ccttaatgtc ttgccagccc agttcccggg cgagtttcgc caaacgctca
     3301 ctgacgacca atagtcgaca gtgtagtaac cagtgctcac gataccattg tgggatcagc
     3361 gaccagagtt gctgcaacat ttcaccgctg gtaacaacga ccatcgtcac ctcgcgggat
     3421 tgccagcgca tcgcttcttc tgcaccatcg taatggattg cgcatcgttg ataacattca
     3481 caaaaagtga cctcagcacc gcgcaccgtc agggtatccc caattagctc acgaccgcca
     3541 ttgccacgta atatcagcgc acgtttgccc gcaatatttt gtaattcagg taattgtagc
     3601 aagacttcgc tgatttcccg atcctgtggg tagagaatct tctgtccgct tacggtatgt
     3661 agcgccagtg cggtggtgcg tccaatggcg aaataagtag gtagttgggg ccatttacga
     3721 tcttgctgat gcagctgtga ttgggcaaaa gcaaccgcgt gttgcgagag ggcaaacaac
     3781 agatcgctct cccccagcgc tgccagttga tcagcaagtt gcggtaattg tcgacccgga
     3841 gaaaactcaa tcagcggaaa atgccaggcc acctgcccca gtgtgcgcag acggctcact
     3901 aactcttctc cagcgggaga cgggcgggtg accaggatac tcatgccggg gcctctccgt
     3961 tatagacttc agcgaggatc tcgcgcgcgc cgttattcag tagctcttct gccagcgaaa
     4021 tccccatttg ttcggcatct tgcggcgcac cgcggcgttc accgcgaata atctgcgaac
     4081 cgtccggcgc gccgaccagc gcacatagcc agatttcgcc atcaataagc tcggcgtagc
     4141 taccaattgg cacctgacat ccgccttcga gacgggtatt catggcgcgt tctgcggtaa
     4201 cgcgcagtgc agtttcgtgg tgattcagcg cggcaagcag ctcgcgagtg cgtgtatcat
     4261 caaggcggca ttcaataccg actgcacctt gtcctaccgc cggaagagaa atctcgggtg
     4321 gtaacgcggc gcgaatgcgg gactccagac ctaaacgttt tagtccggct acggcaagaa
     4381 tgatggcatc gtattcgccg ttatctagtt tgctcaggcg agtgccgacg ttgccgcgca
     4441 gggagcggat aatcagatcc gggcggcgtt cagccagttg gcactggcga cgtaaactgg
     4501 acgtcccgac gatactgcct gctggtaacg catccagact gtcatagtta ttggacacaa
     4561 aggcatcgcg aggatcttca cgctcgcaaa tagtgaccag ccccagacct tgcgggaatt
     4621 caaccggcac atctttcatt gaatgtacgg cgatatcagc gcgattctcc aggagcgcga
     4681 cttccagctc ttttacaaat aagccttttc cgcctacttt cgccagcggc gtatcaagaa
     4741 tcacatcgcc gcgcgtcacc atcggtacca gttcaacgac caggcccgga tggctcgcca
     4801 tcaacttgtc tttgacatag tgtgcctgcc agagtgcaag tgggctttgg cgtgtggcaa
     4861 ttcttaaaac attgtctaac atgcttgtta ccgtcattat catccgtggt ccatcctaac
     4921 atccttgcca gagtgatgtc agtgttgtgg tgaaacgtag acgcctgcgc aaaccgtaaa
     4981 atgaggtctg gcagtggatc ctgacaggcg tttcacgccg tcgtaataag gaatttacag
     5041 agaataaacg gtgctacact tgtatgtagc gcatctttct ttacggtcaa tcagcaaggt
     5101 gttaaattga tcacgtttta gaccattttt tcgtcgtgaa actaaaaaaa ccaggcgcga
     5161 aaagtggtaa cggttacctt tgacatacga aatatcccga atgccgcgtg ttaccgttga
     5221 tgttggcgga atcacagtca tgacgggtag caaatcaggc gatacgtctt gtacctctat
     5281 attgagactc tgaaacagag actggatgcc ataaatcaat tgcgtgtgga tcgcgcgctt
     5341 gctgctatgg ggcctgcatt ccaacaggtc tacagtctac tgccgacatt gttgcactat
     5401 caccatccgc gaatgccggg ttaccttgat ggtaacgttc ccaaaggcat ttgcctttac
     5461 acgcctgatg aaactcaacg ccactacctg aacgagcttg aactgtatcg tggaatgtca
     5521 gtacaggatc caccgaaagg tgagcttcca attactggtg tatacaccat gggcagcacc
     5581 tcgtccgtag ggcaaagttg ttcctctgac ctggatatct gggtctgtca tcaatcctgg
     5641 ctcgatagcg aagagcgcca gttactacaa cgtaaatgca gcctgctgga aagctgggcc
     5701 gcctcgctgg gtgtggaagt cagcttcttc ctgattgatg aaaaccgctt ccgtcataat
     5761 gaaagcggca gcctgggggg cgaagattgt ggctccaccc agcatatact gctgcttgac
     5821 gaattttatc gtactgccgt gcgtctcgcc ggtaagcgta ttctgtggaa tatggtgccg
     5881 tgcgacgaag aagagcatta cgacgactat gtgatgacgc tttacgcgca gggcgtgctg
     5941 acgccaaatg aatggctgga tctcggtggc ttaagctcgc tttctgctga agagtacttt
     6001 ggtgccagcc tttggcagct ctacaagagt atcgattccc catacaaagc ggtgctgaaa
     6061 acactgctgc tggaagccta ttcctgggaa tacccgaacc cacgtctgct ggcgaaagat
     6121 atcaaacagc gtttgcacga cggcgagatt gtatcgtttg gcctcgatcc atactgcatg
     6181 atgctggagc gtgttactga atacctgacg gcgattgaag atttcacccg tctggattta
     6241 gtacgtcgct gcttctattt aaaagtgtgc gaaaaactca gccgtgaacg cgcctgtgta
     6301 ggctggcgtc gcgcagtgtt gagccagtta gtgagcgagt ggggttggga cgaagctcgt
     6361 ctggcaatgc tcgataaccg tgctaactgg aagattgatc aggtgcgtga ggcgcacaac
     6421 gagctgctcg acgcgatgat gcagagctac cgtaatctga tccgctttgc gcgtcgcaat
     6481 aaccttagcg tctccgccag tccgcaggat atcggcgtac tgacgcgtaa actgtatgcc
     6541 gcgtttgaag cattgccagg taaagtgacg ctggtaaacc cgcagatttc acccgatctc
     6601 tcggaaccga atctgacctt tatttatgtg ccgccgggcc gggctaaccg ttcaggttgg
     6661 tatctgtata accgcgcgcc aaatattgag tcgatcatca gccatcagcc gctggaatat
     6721 aaccgttacc tgaataaact ggtggcgtgg gcatggttta acggcctgct gacctcgcgc
     6781 acccgtttgt atattaaagg taacggcatt gtcgatttgc ctaagttgca ggagatggtc
     6841 gccgacgtgt cgcaccattt cccgctgcgc ttacctgcac cgacaccgaa ggcgctctac
     6901 agcccgtgtg agattcgcca tctggcgatt atcgttaacc tggaatatga cccgacagct
     6961 gcgttccgca atcaggtggt gcattttgat ttccgtaagc tggatgtctt cagctttggc
     7021 gagaatcaaa attgcctggt aggtagcgtt gacctgctgt accgcaactc gtggaacgaa
     7081 gtgcgtacgc tgcacttcaa cggcgagcaa tcgatgatcg aagccctgaa aactattctc
     7141 ggcaaaatgc atcaggacgc cgcaccgcca gatagcgtgg aagtcttctg ttatagccag
     7201 catctgcgcg gcttaatccg tactcgcgtg cagcaactgg tttctgagtg tattgaactg
     7261 cgcctttcca gcacccgcca ggaaaccggg cgtttcaagg cgctgcgcgt ttctggtcaa
     7321 acctggggct tgttcttcga acgcctgaat gtatcggtac agaagctgga gaacgccatc
     7381 gagttttatg gcgctatttc gcataacaaa ctgcacggct tgtcagtgca ggttgaaacc
     7441 aatcacgtca aattaccggc ggtggtggac ggctttgcca gcgaagggat catccagttc
     7501 tttttcgaag aaacgcaaga cgagaatggc tttaatatct acattctcga cgaaagcaac
     7561 cgggttgagg tgtatcacca ctgcgaaggc agcaaagagg agctggtacg tgacgtcagt
     7621 cgcttctact cgtcatcgca cgaccgcttc acctacggct caagcttcat caacttcaac
     7681 ctgccgcagt tctatcagat tgtgaaggtt gatggtcgcg aacaggtgat tccgttccga
     7741 acaaaatcga ttggcaacct gccgcccgcc aatcaggatc acgatacgcc gctattacag
     7801 cagtattttt cgtgatgagc gtgccggaaa gcgaggctta tccggcatgc aatcttagcg
     7861 gaaactgact gtttcacccg cctgttgcgt cgccgcctgt tccagcaaat cccagaaggt
     7921 ttcgccgctg cgatcgcaaa tccattcatc gcctttcagg tcaaaatggt agccgccctg
     7981 tttggttgcc agccacacct ggtgcagcgg ctcctggcgg ttaataatga ttttgctgcc
     8041 gttttcaaag gtaatggtca gtacgccgcc gttgatttcg cagtcgatat cgctgtcgcc
     8101 atccctgtcg tccagacgct cttcaatggt cagccacagt tgatcagcca ggcgatgaaa
     8161 ttcactgtcg ttcattgttg tatcctgttt ttaagtgatg gcggcagtat agcggcatgg
     8221 ggtcagggct tcaaagtttg cacctctgct gctgcgttcc ggcatgattc atccgtcacc
     8281 ggaataatga tgtctctgtt ttgaacttca cgtacgttct cctctctgac acgagttttc
     8341 gccgggcagg gggagggcgt aatccttacc tctgtatcgg tccatgtata aacattgcga
     8401 tcgtcataag cgaagcgatc gtttttagtt agcgaaccgt taactggaat aatttgcaag
     8461 ctctttgacc gtaattggcg gataaagtaa gcatgctgtt tgccgctatt aaatgagcga
     8521 tcaatgacca ggtctggcgt atccagcggg attatctcaa gctggtcttc accaaatgtc
     8581 gataattttg ttacatacag ccgatcttta tattgcgcaa tattgccagt aatcagatgg
     8641 aatt
//
LOCUS       DAJTUI010000142         8431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTUI010000142 DAJTUI010000000
VERSION     DAJTUI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.142"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..188
                     /gene="csgC"
                     /locus_tag="OO464_003661"
     CDS             <1..188
                     /gene="csgC"
                     /locus_tag="OO464_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR6228664.1"
                     /translation="SSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSD
                     GQALHLSQQWTPSSEKS"
     gene            309..620
                     /locus_tag="OO464_003662"
     CDS             309..620
                     /locus_tag="OO464_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6228665.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            715..1248
                     /gene="ymdB"
                     /locus_tag="OO464_003663"
     CDS             715..1248
                     /gene="ymdB"
                     /locus_tag="OO464_003663"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR6228666.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1190..2671
                     /gene="clsC"
                     /locus_tag="OO464_003664"
     CDS             1190..2671
                     /gene="clsC"
                     /locus_tag="OO464_003664"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR6228667.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2679..3836)
                     /gene="mdoC"
                     /locus_tag="OO464_003665"
     CDS             complement(2679..3836)
                     /gene="mdoC"
                     /locus_tag="OO464_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6228668.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4230..5765
                     /gene="mdoG"
                     /locus_tag="OO464_003666"
     CDS             4230..5765
                     /gene="mdoG"
                     /locus_tag="OO464_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6228669.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPLPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5758..8301
                     /gene="mdoH"
                     /locus_tag="OO464_003667"
     CDS             5758..8301
                     /gene="mdoH"
                     /locus_tag="OO464_003667"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6228670.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2135 a         1996 c         2197 g         2103 t
ORIGIN      
        1 gcagttcagg gcaaagtcag acgaagcaag aaaagaccct ctcattgcct gctaatcaac
       61 ccattgcttt gacgaagttg agtttaaata tttccccgga agatcgggtg aaaatagttg
      121 ttactgtttc tgatggacag gcacttcatt tatcacaaca atggacgccc tcttcagaaa
      181 agtcttaatt tgttgaaata tcgagcataa gatgaatctg gagagaatgg tctgctgcga
      241 atcagccaac ctgaaagtat ggataacaca accttcaagg atgactaatc attgaggaaa
      301 tagaataaat gttcagacct tttttagact ctcttatgct cggcagtttg ttttttcctt
      361 ttattgccat tgcaggaagc accgcgcaag ggggcgtgat ccatttttat ggccaaattg
      421 tggaaccggc atgtgacgtc agcacccagt catcacccgt agaaatgaac tgcccacaga
      481 atggttctat tccgggcaaa acctactcca gcaaagcgtt aatgagcggc aatgtcaaaa
      541 acgcgcaaat agcctcagta aaagtgcagt atcttgataa acagaaaaag ctggctgtaa
      601 tgaacatcga atataactaa gtttctgaac aactcacgtt gctgagcaga aaaatgcgat
      661 ttaaccaaaa agcctgctgt acacttaaga aacaagaagg tgtaaggagg cgttatgaaa
      721 acgcgtattc atgttgtgca gggtgatatt accaaactgg ccgttgatgt gattgtgaat
      781 gcggctaatc cgtcattaat gggaggcggc ggcgtcgatg gggccattca tcgcgcagcg
      841 ggtccggccc tgctggatgc ctgtttaaaa gtcaggcaac agcagggcga ttgccctacg
      901 gggcatgccg ttattacgct tgcaggcgat cttcccgcta aagccgtagt acacaccgtc
      961 gggccagtct ggcgtggtgg ggaacaaaac gaagaccagc ttttgcagga tgcctatctg
     1021 aatagcctac gactggtggc ggcaaacagc tatacgtcag tggcttttcc tgcaatcagt
     1081 actggggttt atagttaccc tcgtgcggca gcggctgaaa tcgcagttaa aaccgtttca
     1141 gaatttatta cccgtcacgc tttacccgaa caggtatact ttgtctgtta tgatgaagaa
     1201 aacgcccacc tctacgaaag actccttacc caacaaggag atgaatgatt tgccccggct
     1261 ggcgagcgcg gtgctgccac tgtgttcgca acatcccggt cagtgtggtc ttttccctct
     1321 ggagaaaagt ctggatgcgt ttgccgcccg gtatcgtctg gccgaaatgg cagagcatac
     1381 gctggatgtt cagtattaca tctggcagga cgatatgtcg gggcggttac tgttttccgc
     1441 cctgttagcc gcagcaaagc gtggcgttcg cgtccgtttg ttgctggacg acaacaatac
     1501 gcctggactt gacgacattt tgcgcttgct tgacagtcat ccacgcattg aagtccggct
     1561 ttttaatcct ttctcgtttc gcttgctacg tccgcttgga tatatcaccg acttttcccg
     1621 tctcaatcgc cggatgcaca ataaaagttt cactgtcgat ggcgtggtta ccctagtggg
     1681 aggacgaaat attggtgatg cctattttgg tgcaggggaa gagccacttt tttcggattt
     1741 agatgtcatg gcaataggcc ccgtggtaga ggatgttgcc gatgatttcg cccgctactg
     1801 gtattgcaaa tcggtttcac ccttacagca ggtgctggat gtcccggaag gtgaaatggc
     1861 ggatcgcatc gagttacccg cctcctggca taacgatgcc atgacgcatc gttatttacg
     1921 caaaatggaa tccagtccat ttataaatca tctggttgat ggaacgttgc cgcttatctg
     1981 ggcgaagaca cgtttattaa gtgatgatcc ggcgaaaggg gagggcaagg caaaacggca
     2041 ttcactgtta ccgcagcgcc tgttcgatat catgggctca cccagtgaac gcatcgatat
     2101 tatctcttcc tattttgtac cgacacgcgc aggtgtggcg caactcttac ggatggtgag
     2161 aaaaggggta aagattgcga tcctaaccaa ttctcttgcc gctaacgatg ttgctgtcgt
     2221 ccatgccgga tacgcgcgct ggcgcaaaaa attgctccgc tatggcgtgg aattatatga
     2281 actcaagccg acgcgtgaac aaagcagtac gttacacgat cgcggcataa ccggtaattc
     2341 cggtgccagc ctgcatgcta aaacttttag catcgatggt aaaacggtgt ttatcggctc
     2401 tttcaatttc gatccgcgtt caacattgct caatactgaa atgggcttcg tgatagagag
     2461 cgaaacgctg gcacagttaa ttgataaacg ctttattcag agccagtacg atgcggcctg
     2521 gcagctccgt ctggacaggt ggggacggat caactgggtt gatcgtcatg caaagaaaga
     2581 ggttattctc aaaaaagaac ccgccaccag tttctggaag cgggttatgg tcagactggc
     2641 gtcgatattg cccgtggaat ggttattgta aatgtggctt aacgggctgg tgctttatcg
     2701 ttctcacgct taacaaccgg tttaccagag aacaaaaact tcagtaacgg gatgcgtaaa
     2761 tgaatttcat acagaattat cgcaatccct actacgaata tcaggccaca gagaaaacca
     2821 agccagttgg aggtgatgtg cggtgtaatg tatgcgccga aaaacagcgt taacgggtgg
     2881 tgaaccagat agataaacag cgatgcgtta acaaaataag tcacccgcgc tgactggaag
     2941 ttaagcaaac ggtggccaaa ggagaagacc acattcacca tccacaggcc gaggaccatg
     3001 gtgatcaccg actcggtttc gtacatccag gcatcgccac tgccatagcg ctggttgagt
     3061 aaataagcga caaacgccaa tgctgcagcg agggtacagc cacgagacgg cgtggtaaac
     3121 aaggctttaa gatgagggaa aatgaaagcc agtgcgccga ggataaagaa cggtaaataa
     3181 aatagcgttt gcatgacaat aaaattgaac atgccattac tcagaatggg tggatacaca
     3241 ataaaaatcg ttcttcttat taccgcatag ccgatgccga ggcataaaaa aatcaccgat
     3301 agttttacca tcgagaattt tttattcgtt ttatcagaat tttctaaatt atttctgatg
     3361 cgcttaaata tccatacaca cagcgtcgtc atgactacta acaccagtaa aaaccacagg
     3421 tgtgatatta attcccaggc caacgtatta tatttgtcat acaatgatag cccaggccaa
     3481 ctttccgctt tccctttgac atattgcagc ataataaatt gcggtaatgt cagtaggggg
     3541 atggctgtta acatcgggat acctacacgt tcgacacgta ctttccacca ttttttcaag
     3601 ggatagcgta aaaaaagcat gtaggaaaag tatccggata taacgaaaaa tacctgcatg
     3661 cggaacgagt ggatgaagtc attaaaaagg gttagccaca atgacggttc ggcgctattc
     3721 acatgccatg tatggctcga atagattaaa gaaatatgaa aagggatccc taacaacatc
     3781 agccaggcgc ggatggagtc gaggaaatat tcacgttgcg cgggtactgg attcatatat
     3841 ggttaactaa tctcggattt ttcgtcttat ccctgtcggg ttatgccttt aggcttgttg
     3901 ccatagcgac accgacctga ccgcgccagg cgcaggcttc aaggttttta tgcatagcat
     3961 catcgctacc actaaccaga atggaagcgt ctgtaagacg gttgataaat aaatttgctg
     4021 gcaaacccta cacgaagtcg atgcttctgt ctttaggaga agcacggaaa gtgaaaacgg
     4081 ttgcaatcag gtgcttaatc catgagccag cgtgctgaac gataccggga ttcagttgtc
     4141 ggaatggctg gttatccatt aaaatagatc ggatcgatat aagcacacaa agggggaagt
     4201 gcttactaat tatgaaacat aaactacaaa tgatgaaaat gcgttggttg agtgctgcag
     4261 taatgttaac cctgtataca tcttcaagct gggctttcag tattgatgat gtcgcaaagc
     4321 aagctcaatc cttagccggg aaaggctatg aggcgcctaa aagcaacttg ccctccgttt
     4381 tccgcgacat gaaatacgcg gactatcagc agatccagtt taatcatgac aaagcgtact
     4441 ggaacaatct gaagacccca ttcaaactcg agttctacca tcagggtatg tacttcgata
     4501 ccccggtcaa aataaatgaa gtgactgcca ccgcagtcaa acgaatcaaa tacagcccgg
     4561 attatttcac tttcggcgat gttcagcatg acaaagacac ggtaaaagac cttggttttg
     4621 ccggttttaa agtgctttac ccgatcaaca gcaaagataa aaacgatgaa atcgtcagca
     4681 tgctcggggc cagctatttc cgcgtgattg gtgcaggtca ggtttatggc ctttctgccc
     4741 gcggcctagc aattgatacc gctttgccat cgggtgaaga atttccacgc ttcaaagagt
     4801 tctggatcga gcgtccaaaa ccgactgata aacgtttaac catttatgca ttgcttgact
     4861 cgccgcgtgc gacaggtgct tacaaattcg tggttatgcc agggcgtgac acggttgtgg
     4921 atgtgcagtc gaaaatctat ctgcgcgata aagtcggcaa actgggggtt gcaccgttaa
     4981 ccagtatgtt cctgtttggg ccgaaccaac cgttgcctgc aaataactat cgtccggagt
     5041 tgcacgactc taacggtctg tctatccatg ctggtaatgg cgaatggatc tggcgtccgt
     5101 tgaataaccc gaaacattta gcggtcagca gcttctccat ggaaaacccg cgaggctttg
     5161 gtctgttgca acgaggtcgt gatttctccc gctttgaaga tctcgatgat cgttacgatc
     5221 tccgtccaag cgcatgggtg actccgaaag gggagtgggg caaaggcagc gttgagctgg
     5281 tggaaattcc aaccaacgat gaaaccaacg ataacatcgt cgcttactgg acgccggatc
     5341 agctgccgga gccgggtaaa gagatgaact ttaaatacac catcaccttc agccgtgatg
     5401 aagacaaact gcatgcgcca gataacgcat gggtgcaaca aacgcgtcgt tcaacggggg
     5461 atgtgaagca gtcgaacctg attcgccagc ctgacggtac tatcgccttt gtggtcgatt
     5521 ttaccggcgc agagatgaaa aaactgccag aggatacccc ggtcacagcg caaaccagca
     5581 ttggtgataa tggtgagata gttgaaagca cggtgcgcta taacccggtt accaaaggct
     5641 ggcgtctggt gatgcgtgtg aaagtgaaag atgccaagaa aatcactgaa atgcgtgctg
     5701 cgctggtgaa tgccgatcag acgttgagtg aaacctggag ctaccagtta cctgccaatg
     5761 aataagacaa ctgagtacat tgacgcaatg cccatcgccg caagcgagaa agcagcattg
     5821 ccgaagactg atatccgcgc cgttcatcag gcgctggatg ccgaacaccg cacctgggcg
     5881 cgggaggatg attccccgca aggctcggta aaggcgcgtc tggaacaagc ctggccagat
     5941 tcacttgctg atggacagtt aattaaagac gacgaagggc gcgatcagct taaggcgatg
     6001 ccagaagcaa aacgctcctc gatgtttccc gacccgtggc gtaccaaccc ggtaggccgt
     6061 ttctgggatc gcctgcgtgg acgcgatgtc acgccgcgct atctggctcg tttgaccaaa
     6121 gaagagcagg agagcgagca aaagtggcgt accgttggta ccatccgccg ttacattctg
     6181 ttgatcctga cgctcgcgca aactgttgtc gcgacctggt atatgaagac cattcttcct
     6241 tatcagggtt gggcgctgat taatcctatg gatatggttg gtcaggattt gtgggtttcc
     6301 tttatgcagc ttctgcctta tatgctgcaa accggtatcc tgatcctttt tgcggtactg
     6361 ttctgttggg tgtccgccgg attctggacg gcgttgatgg gcttcctgca actgcttatt
     6421 ggtcgcgata aatacagtat atctgcgtca acagttggcg atgaaccatt aaacccggag
     6481 catcgcacgg cgttgatcat gcctatctgt aacgaagacg tgaaccgtgt ttttgctggc
     6541 ttgcgtgcaa cgtgggaatc agtaaaagcc accgggaatg ccaaacactt tgatgtctac
     6601 attctgagtg acagttataa cccggatatc tgcgtcgcag agcaaaaagc ctggatggag
     6661 cttatcgctg aagtcggtgg cgaaggtcag attttctatc gccgccgccg tcgccgcgtg
     6721 aagcgtaaaa gcggtaatat cgatgacttc tgccgtcgct ggggcagcca gtacagctac
     6781 atggtggtgc tggatgctga ctcggtaatg accggtgatt gtttatgcgg cctggtgcgc
     6841 ttgatggaag ccaacccgaa cgccgggatc attcagtcgt cgccgaaagc gtccggtatg
     6901 gatacgctgt atgcgcgctg tcagcagttc gcgacccgcg tgtatgggcc actgtttaca
     6961 gccggtttgc acttctggca acttggcgag tcgcactact ggggacataa cgcgattatc
     7021 cgcgtgaaac catttatcga gcactgcgca ctggctccgc tgccgggcga aggttccttt
     7081 gccggttcaa tcctgtcaca tgacttcgta gaagcggcgt tgatgcgccg tgcaggttgg
     7141 ggggtctgga ttgcttacga tctcccgggt tcttatgaag aattgccgcc taacttgctt
     7201 gatgagctaa aacgtgaccg ccgctggtgc cacggtaacc tgatgaactt ccgtctgttc
     7261 ctggttaagg gtatgcaccc ggttcaccgt gcggtgttcc tgacgggcgt gatgtcttat
     7321 ctctccgctc cgctgtggtt tatgttcctc gcgctctcta ctgcattgca ggtagtgcat
     7381 gcgttgaccg aaccgcaata cttcctgcaa ccacggcagt tgttcccggt gtggccgcag
     7441 tggcgtcctg agctggcgat tgcacttttt gcttcgacca tggtgctgtt gttcctgccg
     7501 aagttattga gcattttgct tatctggtgc aaaggaacga aagaatacgg cggcttctgg
     7561 cgcgttacat tatcgttgct gctggaagtg ctgttttccg tgctgctggc tccggtacgc
     7621 atgctgttcc atacggtctt cgttgtcagc gcgttccttg gctgggaagt ggtgtggaat
     7681 tcaccgcagc gtgatgatga ctccacttca tggggcgaag cgttcaaacg ccacggctca
     7741 cagctgctgt tggggttagt gtgggctgtt gggatggcgt ggctggatct gcgtttcctg
     7801 ttctggctgg caccgattgt cttctcgttg atcctgtcac cgtttgtttc ggtgatttcc
     7861 agccgtgcca ccgttggtct gcgcaccaaa cgctggaaac tgttcctgat cccggaagag
     7921 tattcaccgc cgcaggtgct ggttgatacc gatcggttcc ttgagatgaa tcgccaacgc
     7981 tcccttgacg atggctttat gcacgcagtg tttaacccgt catttaacgc tctggcaacc
     8041 gcaatggcga cagcgcgtca ccgcgccagt aaggtgctgg aaatcgcccg tgaccgccac
     8101 gttgaacagg cgcttaacga gacgccagag aagctgaatc gcgatcgtcg cctggtgctg
     8161 ctaagcgatc cggtgacgat ggcccgtctg catttccgcg tctggaattc ccctgaaaga
     8221 tattcttcat gggtgagtta ttacgaaggg ataaagctca atccactggc attgcgtaaa
     8281 ccggatgcgg cttcgcaata aaaacgtagt tgcctgatgc gctacgctta tcaggcctac
     8341 atcgttcctg caatttattg attttgcaag attttgtagg tcggataagg cgttcacgcc
     8401 acatccggca aaaacaacga gccaataaaa a
//
LOCUS       DAJTUI010000143         8366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTUI010000143 DAJTUI010000000
VERSION     DAJTUI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.143"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..948
                     /gene="espL1"
                     /locus_tag="OO464_003668"
                     /pseudo
     CDS             1..948
                     /gene="espL1"
                     /locus_tag="OO464_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1120..1227
                     /locus_tag="OO464_003669"
     CDS             1120..1227
                     /locus_tag="OO464_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228671.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1280..2038)
                     /locus_tag="OO464_003670"
     CDS             complement(1280..2038)
                     /locus_tag="OO464_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR6228672.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2325..3254
                     /gene="pfkB"
                     /locus_tag="OO464_003671"
     CDS             2325..3254
                     /gene="pfkB"
                     /locus_tag="OO464_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6228673.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3355..3645
                     /gene="ghoS"
                     /locus_tag="OO464_003672"
     CDS             3355..3645
                     /gene="ghoS"
                     /locus_tag="OO464_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6228674.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3751..4611
                     /locus_tag="OO464_003673"
     CDS             3751..4611
                     /locus_tag="OO464_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6228675.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4652..5188)
                     /locus_tag="OO464_003674"
     CDS             complement(4652..5188)
                     /locus_tag="OO464_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6228676.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5335..6003
                     /gene="hxpB"
                     /locus_tag="OO464_003675"
     CDS             5335..6003
                     /gene="hxpB"
                     /locus_tag="OO464_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR6228677.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6166..6756
                     /locus_tag="OO464_003676"
     CDS             6166..6756
                     /locus_tag="OO464_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6228678.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6889..8279
                     /gene="tcyP"
                     /locus_tag="OO464_003677"
                     /pseudo
     CDS             6889..8279
                     /gene="tcyP"
                     /locus_tag="OO464_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT         2168 a         2002 c         2008 g         2188 t
ORIGIN      
        1 cctgggctga taggtatgtt cgcaactgtg caattgctat tctgccccta ttacctgtgg
       61 gagttctagg ggctattttc gcgggtattg catcacgaat gccatttgcc cctatacatc
      121 catcggcaat gttgttaata atggctacag gccagactca acagcttatt acattattca
      181 aacagttgcc catactccct gaaaaagaaa tcattgaaat aataactgcg cagaatagcg
      241 ttggtacacc tgctttattt ttggctatga tgaacggaca tactgacaac gtgaaaatat
      301 ttatgcaaga aattcagtca ctggtagata atcacatcat tcatgaagat aatctggtta
      361 aattactgca aactaaaagt gctaacgaaa cacctggact ttatatctcc atgttgtatg
      421 gattcgatga aataatcgat atctttctga atacattagc cactccaata gcactaagag
      481 cttttaaaca aaaaactggt gatgagtatt ttagccatga aaatacatga tggtgagcca
      541 ggattatacg ccgcaatgga aaataatcac cctttgtgtg tcacacggtt cctctctaaa
      601 attaatggca tcgcctttaa atacaagttg agcaaagcta acatcatgga tttattaaaa
      661 ggagctacag cacagggaac ccctgcatta tacatcgcca tgagcaaggg taatgaagac
      721 gtcgtgttat cttatatatc gacgctgggt gcttttgcaa aaaaacattc ttttagtcaa
      781 catcagttat ttacactatt ggccgctaaa aatcatgaca acatgtcagc tgttcatata
      841 gccattcatc ataagcatta taaaactgta gaaacatatt atgctgctat aaatgtcatc
      901 agccaaagcc tgagttttag tgctgatgaa ataaagacgt atttataacc agctatttac
      961 gatatatatc gatacataat tttatttttt tcataaaacg cagaaacagt gaccatgtgc
     1021 acaacgccac ccgacggctg taataacaaa aaatcccttg atgcctgtcc cttttgttac
     1081 actccgttat cacgcacaag agatatgcag gacactggta tgccgactaa acgctttgat
     1141 aaaaaacact ggaagatggt ggtggtgcta ctggcaatct gtggcgctat gttgttgcta
     1201 cgttgggcag caatgatttg gggctgagca gtgacaaacg aagacagcca gaccgcataa
     1261 cacggtccgg cagataattt tacatgctgt agcccagcga taaggttgtc cgacggtcgg
     1321 tatgttccgg tgcagactct ggaggttcag agttccatgt gacgttgtaa gccaccttca
     1381 gtccaaaatg ttcattaata gcaacattta atgcgctttc ggagttcagc gttgtgtctt
     1441 ccgcgccaaa gaccgaaaca ccctgcgtaa atttagcgtt gtcagtcaac tgccatgcat
     1501 aagccccgga ggcatagccc agcggctggg tttcactggc attgtcggta tatttgtcgt
     1561 aacgcacacc tggaccgaat tcaaagcgga aactgtgtac cgggccattc aaaaactgac
     1621 gaccataacc cgcggtcaac acatcgcgct ctcgatagcc gttataacgg tctgtcagcc
     1681 agcttgcctg accaaataaa tagtcataat cagttaaatt aaagcggcta cgcccgcccg
     1741 ccgcatattt ttctgaagaa cgctcatcat tagaagaagt attactggcg ttcccccaca
     1801 gcgaccaggc agtggtttgt ccataccagg tcatggtggt gtcagccgta agtgaggagc
     1861 ttttcgtatt gcctgattgt gcaagatatc ctgcgttcag attaccttcg aaaggttttt
     1921 tagcgctggc aggatcgtcc atgacagtaa aaacggaatc atcggcagct gcattcagtg
     1981 acgcaaacat gccccccgcc aacataacga tggcaggaac tgtcttcaaa agcttcattt
     2041 atcaagagtc cgtacaacaa aaaaagagac catcgcggtc ccggaaactt tcttaaggat
     2101 caaagattag cgtccctgga aaggtaacga attataaaaa ggcgcgaata acttagcaat
     2161 gtattcttat ttcatttttt gaataagcat gtggcgaaaa cagattttta tttatatata
     2221 tttatctgca aaattttaaa taaagctcta ataaatcata ttgttaattt cttcactttc
     2281 cgctgattcg gtgccagact gaaatcagcc tataggagga aatgatggta cgtatctata
     2341 cgttgacact tgcgccctct ctcgatagcg caacaattac cccgcaaatt tatcccgaag
     2401 gaaaactgcg ctgtaccgca ccggtgttcg aacccggggg cggcggcatc aacgtcgccc
     2461 gcgccattgc ccatcttgga ggcagtgcca cagcgatctt cccggcgggt ggcgcgaccg
     2521 gcgaacacct ggtttcactg ttggcggatg aaaatgtccc cgtcgctact gtagaagcca
     2581 aagactggac ccggcagaat ttacacgtac atgtggaagc aagcggtgaa cagtatcgtt
     2641 ttgttatgcc aggcgcggca ttaaatgaag atgagtttcg ccagcttgaa gagcaagttc
     2701 tggaaattga atccggggcc atcctggtca taagcggaag cctgccgcca ggtgtgaagc
     2761 tggaaaaatt aacccaactg atttccgctg cgcaaaacca agggatccgc tgcatcatcg
     2821 acagttctgg cgaagcgtta agtgcagcac tggcgattgg taacatcgag ttggttaagc
     2881 ctaaccaaaa agaactcagt gcgctggtga atcgcgaact cacccagcca gacgatgtcc
     2941 gcaaagccgc gcaggaaatc gttaatagcg gcaaggccaa acgggttgtc gtttcccttg
     3001 gtccacaagg agcgctgggt gtcgatagtg aaaactgcat tcaggtggtg ccaccaccgg
     3061 tgaaaagcca gagtaccgtt ggcgctggtg acagcatggt cggcgcgatg acactgaaac
     3121 tggcagaaaa tgcctctctt gaagagatgg tccgttttgg cgtagctgcg gggagtgcag
     3181 ccacactcaa tcagggaaca cgtctgtgct cccatgacga tacgcaaaaa atttacgctt
     3241 acctttcccg ctaacaaaaa cattccccca gcattggggg aatcaccacc aacctgtcgg
     3301 caacgcgttt ctccgactat gctcaaaagt catgtgataa caaaggggtg aactatggcc
     3361 agtggcgatc ttgtccatta tgtcataacc gtcatgttgc atgaggatac attgactgaa
     3421 attaacgagt tgaataatta cctgactcgc gacggttttt tgctcaccat gacggatgat
     3481 gagggaaata tccatgagct ggggactaac acttttggac ttatcagtac ccaaagtgaa
     3541 gaagaaatta gagaactggt ttcggggctt acccaaagtg caaccggcaa agatcctgaa
     3601 atcaccatca cgacctggga ggaatggaat agcaacagaa aataaatggt ttttgggcaa
     3661 taatcagtct gtggtgtgcg ttagctcgtg tttttacacc gcattcttgc gctaacctta
     3721 tgatctggca gacaacatgg gagagacatc atgtggcagg caatcagtcg tcttttgagc
     3781 gagcagttag gtgaaggcga aatcgaactg cgtaatgaac tgcctggcgg agaagtccat
     3841 gccgcatggc atttgcgcta tgcaggacat gactttttcg tcaaatgtga tgaaagggaa
     3901 ctgcttcccg gttttaccgc cgaagccgac caactggagt tactgtcgcg tagtaaaacc
     3961 gtcaccgtgc ctaaggtttg ggcagtaggc gctgaccgtg actacagttt tctggtgatg
     4021 gattatctcc cacctcgtcc gctggatgcg catagcgcat ttattcttgg tcagcaaatt
     4081 gcgcgtttac atcaatggag tgaccaacca caatttggcc tcgattttga taactcgctc
     4141 tccacaactc cacagcccaa cacctggcaa cgtcgctggt caacgttttt tgctgaacaa
     4201 cggattggct ggcagttgga actggcagca gagaaaggga tcgctttcgg caatatcgac
     4261 gccatcgtcg agcatattca gcagcgtctg gcctcgcatc aaccgcagcc ttctctgttg
     4321 cacggcgatt tatggtccgg caactgtgcg ctgggtccgg atggcccgta cattttcgac
     4381 cctgcctgcg actggggtga ccgagagtgc gacctagcga tgttaccgct gcatactgaa
     4441 cagccgccac aaatctatga cggctatcag tcagtatccc cgctacctgc cgatttcctt
     4501 gaacgtcaac cggtttacca actctacacg ctgttaaatc gtgcaagatt atttggcggt
     4561 cagcatttgg ttattgctca gcagtcattg gatagattat tagcagcatg atatgggttg
     4621 aggataatgg ccgctccgtg cggccttttg attaaataaa cccaagcaga gaaaagaaca
     4681 cataacctgc agcaatacaa ataaccggca ggatatatag tgagaaaaac tgcaggaaaa
     4741 tagtatggtg cggaacaaca atacgggact caatttgttc acgcgttagc ccctcttccc
     4801 ctttggcttt ttccagaatg agttgatctt caacaccttc ccgcaagaag cgcgcctggc
     4861 gactcatccg ggcaccggaa tcctgcaacg ccagtccgac aaaaatcagg atgaaaatca
     4921 cccagaacat aacgttcaca ccaccattaa aattaggggt cggggagtta taccagaaca
     4981 gattcaaaaa aggcgtattc gcctgcatca tatcgatcat gacatgagca aagtcgagca
     5041 tgaccgcatt aatgccttcc tggttttcct gccgggtatt cataaatttc agcaaagaaa
     5101 tcaatgtaga gatcaatgca ggtataaaaa tcacccaacc caaaatcctt ttcaaaacag
     5161 caatgcgtcc agcttgttga tacgtcatga gttctccttg attaagacgc gtcgtttcac
     5221 ttagtttacc tgtagatatc tgttttcgcc cattctttaa aggcgatatg ataggcgctt
     5281 aatcataagc acggcttaat accttacaca taatgctcta aaggagaggt tgtaatgtca
     5341 accccgcgtc agattcttgc tgcaattttt gatatggatg gattacttat cgactcagaa
     5401 cctttatggg atcgagccga actggatgtg atggcaagcc tgggggtaga tatctcccgt
     5461 cgtaacgagc tgccggacac cttaggttta cgcatcgata tggtggtcga tctttggtac
     5521 gcccggcaac cgtggaatgg gccaagccgt caggaagtag tagaacgggt tattgcccgt
     5581 gccatttcac tggttgaaga gacacgtcca ttattaccag gcgtgcgcga agctgttgcg
     5641 ttatgcaaag aacaaggttt attggtggga ctggcctccg cgtcaccact acatatgctg
     5701 gaaaaagtgt tgaccatgtt tgacttacgc gacagtttcg atgccctcgc ctcggctgaa
     5761 aaactgcctt acagcaagcc gcatccgcaa gtatatctcg actgcgcagc aaaactgggc
     5821 gttgacccac tgacctgcgt ggcgctggaa gactcggtaa atggcatgat cgcttctaaa
     5881 gcagcccgca tgcgttccat cgtcgttcct gcgccagaag cgcaaaatga tccacgtttt
     5941 gtattagcaa acgtcaaact ctcatcgctg acagaactca ccgcaaaaga ccttctcggt
     6001 taatgaccag gggcagtgat cacgctgccc ctggttcttt atctgaattt gcattcaact
     6061 gacggattaa tcgtcaattt aagagaaaga gttacaccgt caccacttcc gtgcactgta
     6121 taaaaatcct atactgtacg tatcgacagt ttagtgagtt ttatcatgac ggcggaaggt
     6181 caccttctct tttctattgc ttgtgcggta tttgccaaaa atgccgagct gacgcccgtg
     6241 ctggcacagg gtgactggtg gcatattgtc ccttccgcaa tcctgacgtg tttgttaccg
     6301 gacatcgatc acccaaagtc gtttcttggg cagcgattaa aatggatatc aaaaccgatc
     6361 gcccgcgctt ttgggcatcg tggttttacc cacagtctgc tggcggtatt tgcgctgctg
     6421 gcaacctttt accttaaggt tccggaaggc tggttcattc cggctgatgc gctacaagga
     6481 atggtgctgg gttatttgag ccacatactt gccgatatgc tgacacccgc cggtgttccc
     6541 ctgctctggc catgccgctg gcgtttccgc ttgcctatcc tggttcccca aaagggcaac
     6601 caactggaac gttttatctg catggcatta tttgtctggt cggtatggat gccccattca
     6661 ttacccgaga acagcgctgt tcgttggtca tcgcaaatga tcaatacctt gcagatccag
     6721 tttcatcggc ttattaagca tcaggttgaa tactaaaaag gcaaaaatca cctttctgga
     6781 ataagcaatt ccatttgaat ataagagcca gctcacagtt ctgttaatct tgcgccaaca
     6841 ctatgactgc tacgcagtga tagaaataat aagatcagga gaacggggat gaactttcca
     6901 ttaattgcga acatcgtggt gttcgttgta ctgctgtttg cgctggctca gacccgccat
     6961 aaacagtgga gtctggcgaa aaaagtgctg gtgggtctgg tgatgggtgt ggtttttggc
     7021 cttgccctgc ataccattta tggttctgac agccaggtac ttaaagattc tgtgcagtgg
     7081 tttaacatcg ttggtaacgg ctatgttcaa ctgctgcaaa tgatcgttat gccgttagtc
     7141 ttcgcctcta ttctgagcgc ggttgcccgt ctgcataacg catctcagtt aggcaaaatc
     7201 agttttctga ccatcggtac gcttttgttt accacgctga ttgcggcgct ggtcggtgtg
     7261 ctggtcacca acctgtttgg tttgacggct gaaggtctgg ttcagggtgg tgcagaaact
     7321 gcacgtctga acgccattga aagtaactat gttggtaaag tctctgatca gagcgttccg
     7381 cagctggtct tgtcctttat cccgaaaaac ccgtttgccg atcttaccgg agccaatccg
     7441 acgtcaatta tcagcgtggt aatttttgcc gcattcctcg gcgtagctgc attgaaactg
     7501 ctgaaggatg atgcgccgaa aggggaacgc gtcttaaccg ctatcgatac cctacaaagc
     7561 tgggtgatga aactggttcg ccttgtcatg cagttgaccc cttacggcgt tctggctcta
     7621 atgaccaaag tggttgcagg ttctaacttg caagacatca tcaaactggg aagtttcgtt
     7681 gtcgcgtcct acctcggtct gctgattatg tttgctgtgc atggcattct gctgggcatt
     7741 aatggcgtga gtccgctgaa gtacttccgt aaggtatggc ctgtgctgac gtttgccttt
     7801 accagccgtt ccagtgctgc gtctatccca ctgaatgtgg aagcacaaac gcgtcgtctg
     7861 ggcgttcctg aatccatcgc cagtttcgcc gcctctttcg gtgcaaccat tggtcagaac
     7921 ggctgcgccg gtttgtatcc ggcaatgctg cggtgatggt tgcgcctacg gttggcatta
     7981 acccgctggg cccgatgtgg attgcgacgc tggtcggtat tgttaccgtt agttccgcag
     8041 gcgttgccgg tgtcggtggt ggtgcaactt tcgccgcact gattgtgctg cctgcgatgg
     8101 gcctgccagt aaccctggtg gcgctgttaa tctccgttga accgcttatc gacatgggcc
     8161 gtacggcgct aaacgttagt ggctcgatga cagctggcac gctgaccagc cagtggctga
     8221 agcaaaccga taaagccatt ctggatagcg aagacgacgc cgaactggca caccgttaat
     8281 tcttatgctg gcaacggtcc gttttgtata ggggccgttg ctttactttt taaatgttcc
     8341 atcgcggcga tgcatactga ctcata
//
LOCUS       DAJTUI010000144         8333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTUI010000144 DAJTUI010000000
VERSION     DAJTUI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.144"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..158
                     /locus_tag="OO464_003678"
     CDS             <1..158
                     /locus_tag="OO464_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228679.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(167..565)
                     /gene="vapC"
                     /locus_tag="OO464_003679"
     CDS             complement(167..565)
                     /gene="vapC"
                     /locus_tag="OO464_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR6228680.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(565..792)
                     /gene="vapB"
                     /locus_tag="OO464_003680"
     CDS             complement(565..792)
                     /gene="vapB"
                     /locus_tag="OO464_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR6228681.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            874..1245
                     /locus_tag="OO464_003681"
     CDS             874..1245
                     /locus_tag="OO464_003681"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR6228682.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1266..6127
                     /gene="traI"
                     /locus_tag="OO464_003682"
                     /pseudo
     CDS             1266..6127
                     /gene="traI"
                     /locus_tag="OO464_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6147..6893
                     /gene="traX"
                     /locus_tag="OO464_003683"
     CDS             6147..6893
                     /gene="traX"
                     /locus_tag="OO464_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR6228683.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6948..7508
                     /locus_tag="OO464_003684"
     CDS             6948..7508
                     /locus_tag="OO464_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR6228684.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7639..7851
                     /locus_tag="OO464_003685"
     CDS             7639..7851
                     /locus_tag="OO464_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR6228685.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8095..>8333
                     /locus_tag="OO464_003686"
     CDS             8095..>8333
                     /locus_tag="OO464_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6228686.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1913 a         2086 c         2671 g         1663 t
ORIGIN      
        1 tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga ggcatggcag caggaaaatc
       61 atccggacac ctggcagcag atgcagcgtc gtgaagaggt gaacatcaat gtgcaccggg
      121 aacgcgggga ggatgttgag ccgggagatg atttctgatg aacaggtcag ctccagtctt
      181 cagttctcag gccgcccaca cgttcaaatt cccgggtgtt attagtcaca ataatcagcc
      241 cccgactgcg ggcatgacct gcgatcattt gatcaaatgg cccgacaggg cgtccctgac
      301 gggcaagttc tgctcttatc tggccggtgt gtgtggctgc agcagcgtcg taatccagaa
      361 catcaatgcg ggaaacaaat ccctcgatca cagcgagatt acgttcaggc atctggcttt
      421 tttctgcacc atatatcagc tccatcaggg tgaccgaact gatacacatt ttcccctggt
      481 tcaggttaaa acgctccctg acgctggcgg gtttgttctt tatcgtaaaa atgcagatgt
      541 tggtatcgag cataaacttc agcatcagaa tgactccctt tcttgcatgc cgggctgttc
      601 cctgttatcc ataaaatcgg tgctgacgct gtgtccgtcg aaccattcgt cccatgtctc
      661 tccggctggc gtaatgattc tggttcgtcc gacagcaatc acttcaacgc gttttacgtt
      721 ttccggcaat gcaaccgctt ttggcagtct gaccgcctgg ctgcggttgc tgagaaatac
      781 ggtggtttcc attatttgcc tccttatgtg gatatgtctt gtgtatatct attgtgggat
      841 tgataccggg atatgtcaaa gggatatacg tttatgatga gtatcgcgca ggtcagatcg
      901 gccggaagtg ccggtaatta ttataccgac aaggataatt actatgtgct gggcagcatg
      961 ggagaacgct gggccggcca gggggctgaa caactggggc tgcagggcag tgtcgataag
     1021 gatgtcttta cccgccttct ggagggcagg ctgccggacg gggcggattt aagccgcatg
     1081 caggatggca gtaacaggca tcgtcccggc tacgacctga ccttctccgc tcccaaaagt
     1141 atctccatga tggccatgct cgggggggac aaacgtctta ttgaagcaca taaccaggcc
     1201 gtggattttg ctgtccgtca ggtggaggcc agtgcctcca cctgacggac ggacagtcag
     1261 aaacggtgct gaccggtaat ctggtgatgg cactgtttaa ccacgacacc agtcgcgatc
     1321 aggaaccaca gttacacacg catgcggtgg tgactaatgt tacgcagtat aacggcgaat
     1381 ggaagacact gagcagtgac aaagtgggga aaacggggtt cattgagaat gtgtacgcta
     1441 atcagattgc ctttggcagg ctctaccggg aaaaactgaa agagcaggtt gaggcgctgg
     1501 gctatgaaac tgaagtggtc ggtaagcacg gtatgtggga aatgccgggt gtaccggtgg
     1561 aggccttttc cggacgctca cagactatcc gggaggccgt cggggaagac gcctcgctga
     1621 aatcccggga tgtggcggcg ctggatacgc gtaaatccaa acagcacgtc gatcctgaag
     1681 tcagaatggc cgagtggatg cagacgctga aggaaaccgg gttcgacatc cgggcatatc
     1741 gtgacgcagc ggaacaacgg gcatataccc gcacgcagac acccggaccc gcttcacagg
     1801 acgggccgga tgtgcagcag gcggtgacac aggccattgc cggattaagt gaacgcaaag
     1861 tgcagtttat gtatacggac ctgctggcca ggacggtcgg catactgccg ccggaaaatg
     1921 gtgtgattga acgggcacgc gccggtatcg atgaggccat cagccgtgag cagcttatcc
     1981 ccctcgatcg tgagaagggg ctgttcacgt tcgggattca tatgctcgat gagctgtcag
     2041 tccgggcact cagtcgtgac atcatgaaac agaaccgggt gaccgtacat ccggagaaaa
     2101 gtgtcccccg gacggccggt tacagcgatg ccgtcagtgt tctggcacag gaccgtccgt
     2161 cgctggccat tgtgtccggg cagggcggtg cagccgggca gcgtgagcgg gtggctgaac
     2221 tggtcatgat ggcccgggag caggggcggg aggtgcagat tatcgctgct gaccgtcgct
     2281 cgcagatgaa cctgaagcag gatgaacggc tgtccggtga actgataacc ggacgtcgtc
     2341 agttgctgga aggaatggcc ttcacgccgg gcagtaccgt tatcgtcgac cagggcgaaa
     2401 aactctccct gaaagagacg ttaaccctgc tggatggtgc cgcacgtcat aacgtacagg
     2461 tcctgataac cgacagcggg cagcgaaccg gtacaggcag tgcactgatg gccatgaagg
     2521 atgccggggt gaacacatat cgctggcagg ggggagaaca gcgaccggcc accatcatca
     2581 gtgaaccgga ccgtaatgtc ggctggccgg agattttgcg gccagcgtga aagccggaga
     2641 agagagcgtg gcacaggtca gcggagtacg ggaacaggcc atactgacac aggccattcg
     2701 cagtgagctg aaaacacagg gcgtgctcgg acacccggag gtaaccatga ccgccctgtc
     2761 accggtctgg ctggacagcc ggagccgtta tctgcgggat atgtaccgcc cgggaatggt
     2821 gatggagcag tggaacccgg agacacgcag tcatgaccgc tatgtgacag aacgtgtgac
     2881 ggcgcagagt cacagcctga ccctgcggaa tgcgcagggc gaaacgcagg tggtgcgtat
     2941 ttcttccctg gacagcagct ggtcgctgtt ccggccggaa aaaatgccgg tggcagacgg
     3001 tgagcgtctg agggtgacag ggaaaattcc cggactccgc gtctccggcg gtgaccgcct
     3061 gcaggtggca tccgtcagtg aagatgcgat gacggttgtt gtgccgggac gggctgaacc
     3121 ggccaccctg cctgtgagcg attcaccgtt cactgcactg aagctggaga acggctgggt
     3181 ggaaacgccc gggcattccg tcagcgacag tgcgacggtt tttgcctccg tcacacagat
     3241 ggcaatggac aatgccaccc tgaatggtct ggcccgcagt ggtcgtgatg tccggctgta
     3301 ttcctcactg gatgaaaccc gtactgcgga aaaacttgcc cgccatccct cctttacggt
     3361 ggtttctgag cagataaagg cgcgtgccgg tgagacattg ctggaaaccg ctatcagtct
     3421 gcagaaagcc gggcttcaca cgccggcaca gcaggccatt catctggccc ttcctgtgct
     3481 ggaaagtaaa aacctggcct tcagcatggt ggacctgctg acagaggcga agtcgtttgc
     3541 tgcagaagga accggttttg ctgacctggg aggggaaatc aatgcgcaga taaaacgggg
     3601 tgatttactg tatgtggatg tggcaaaagg ctatggcaca ggcctgctgg tttcccgtgc
     3661 gtcgtatgag gcagaaaaga gcattcttcg ccatattctc gaaggtaagg aggcggtcac
     3721 gccgctgatg gagagagtac ctggcgaact catggagaaa ctgacatcag gacagcgcgc
     3781 cgccacccgt atgatactgg aaacgtctga ccgtttcacg gtggtgcagg gctatgccgg
     3841 tgtgggtaag accacacagt tccgggcggt gatgtcagcc gtgaacatgc tgccggagag
     3901 tgaacgtccc cgtgtcgtgg ggctggggcc cacacaccgt gcggtcgggg agatgcgcag
     3961 cgccggcgtg gatgcacaga cactggcgtc ctttctgcat gacacgcagc tgcagcagcg
     4021 cagcggagaa actccggatt tcagcaacac gctgttcctt ctcgatgaga gctctatggt
     4081 gggcaatacc gacatggcac gggcatacgc cctgattgcg gccggtggcg gtcgggctgt
     4141 ggccagcggt gacacggacc agctgcaggc catcgcgccc ggtcagcctt tccgtctcca
     4201 gcagacgcgc agtgctgccg atgtggtcat catgaaggag attgtgcgtc agacgccgga
     4261 actgcgggag gcggtataca gcctgattaa ccgggatgtg gaaagggcgc tgtccgggct
     4321 tgagcgtgtg aaaccgtctc aggtgccacg tctggagggc gcatgggcac cggagcactc
     4381 cgtgacggag ttcagtcaca gccaggaagc gaaactggca gaagcgcagc agaaggcgat
     4441 gctgaaaggc gaggcttttc cggatgtccc catgacactg tatgaagcca ttgtccgcga
     4501 ctataccggc aggacaccgg aagcacggga gcagacgctg attgtcacgc acctgaatga
     4561 ggaccggcgc gtactgaaca gcatgattca tgatgcacgg gaaaaggccg gagaactggg
     4621 gaaggtgcag gtcatggtgc ctgtcctgaa cacagcgaat atacgcgacg gggagctgcg
     4681 tcgtctctcc acctgggaga ataacccgga tgcccttgcc ctggtggata atgtgtatca
     4741 ccggattgcc ggtatcagta aggatgacgg gctgataacc ctgcaggatg cggaaggtaa
     4801 cacgcggctg atttcgcccc gggaggcggt ggctgaaggc gtcacgctgt acaccccgga
     4861 caccatccgg gtggggaccg gtgacaggat acgcttcacg aagagtgacc gggagcgcgg
     4921 ttatgtggcc aacagcgtct ggacggtgac agcggtttcc ggtgacagtg tcacactttc
     4981 tgacggtcag cagacccgtg tgattcgccc cggtcaggag cgggcagagc aacatattga
     5041 cctggcctat gccatcaccg cccacggtgc acagggggcg agtgaaacct ttgccatcgc
     5101 gcttgaaggc acggaaggta accggaaact gatggccggc tttgagtcag cctacgtggc
     5161 cctgtcgcgt atgaagcagc atgtgcaggt gtacaccgat aaccgtcagg gctggacgga
     5221 tgccatcaac aatgccgtac agaaaggaac tgcccacgat gtgtttgagc cgaaaccgga
     5281 ccgggaggtc atgaatgcag agcggctgtt cagtacggcg cgggagctgc gggacgtggc
     5341 ggcaggccgt gccgttctcc gtcaggcggg gctggccggg ggagacagtc ctgcacggtt
     5401 tattgctccg ggacgtaaat atccgcaacc gtatgtggca ctgccggcgt ttgaccgtaa
     5461 cggcaagtcc gccggtatct ggctgaaccc gctgaccacg gatgacggaa acgggctgcg
     5521 gggattcagc ggtgaaggtc gggtaaaagg cagcggggat gcgcagttcg tggccctgca
     5581 gggcagccgt aacggagaaa gcctgctggc tgataatatg caggatggcg tccggattgc
     5641 ccgtgataat cctgacagtg gtgtggtggt aagaatcgcc ggtgaaggcc gtccgtggaa
     5701 tcccggtgcc atcaccggtg gccgcgtgtg gggggatatc ccggacaaca gcgtccagcc
     5761 gggagccgga aatggtgaac cggtcacggc agaggtactg gcacagcggc aggctgaaga
     5821 ggccatccgc cgtgaaacgg aacgtcgcgc agatgaaatt gtccgtaaaa tggcagagaa
     5881 caaacctgac ctgccggacg gcaaaacaga gcaggctgtc agggaaattg ccgggcagga
     5941 gcgtgaccgg gctgccataa ctgaacggga agccgcgctg ccggagagtg tactgcgtga
     6001 accacaacgg gtgcgggaag cggtccggga agttgcccgg gaaaatttgc tgcaggagcg
     6061 tctgcagcag atggagcggg atatggttcg tgacctgcag aaagagaaaa ccccgggcgg
     6121 agactgatac aggaagacaa acactgatga caacggataa cacgaacacg acacgtaacg
     6181 attcactggc tgcacggacc gatacctggt tgcagtcatt tctggtctgg tcacccggac
     6241 agcgggatat catcaaaacg gtggcactgg tgctgatggt actggaccac atcaatctga
     6301 tattccagct gaagcaggaa tggatgtttc tggccgggcg aggagccttt ccgctttttg
     6361 ccctggtgtg ggggctgaat ctgtcccgtc atgcgcatat ccggcaaccg gccattaacc
     6421 ggctgtgggg atggggaatt attgcccagt tcgcatatta tctggcaggc tttccttggt
     6481 atgaggggaa tatcctgttt gcctttgcag tggcggcaca ggtgctgacg tggtgtgaaa
     6541 cgcgcagtgg gtggcgtaca gcagcggcca tactcctgat ggccctgtgg gggcctttgt
     6601 ccggcaccag ttacggtatt gccgggttgc tgatgctggc agtcagcaac cggctgtacc
     6661 gggcggaaga cagagcggaa cgtctggcgc tggtcgcctg cctgctggct gttattccgg
     6721 cgcttaacct tgccaccagt gatgcagcgg cggtggccgg tcttgtgatg acggtgctga
     6781 ccgttggtct ggtgttgtgt gccggaaaat cattaccccg tttctggccc ggagactttt
     6841 tcccgacgtt ctatgcctgt catctggctg tgctgggcgt tctggcgctg tgacgggtgt
     6901 ggtatctttg gccgcaagag gatgattcgt cagaggcaga acacagcatg acagagcaga
     6961 agcgaccggt actgacactg aagcggaaga cagagggaac agcgcctgtc cgcagccgga
     7021 aaaccatcat caatgtcacc acgccaccaa agtggaaggt gaaaaagcag aagctggccg
     7081 agaaagcggc ccgtgaagca gagctggcgg caaaaaaagc gcaggccaga caggcgctgt
     7141 ccatttatct gaacctgccc acgctggatg aggctgtgaa taccctgaag ccctggtggc
     7201 cgggattatt tgacggtgac acgccccggc ttctggcctg cggtatccgg gacgtgttac
     7261 tggaagacgt ggcgcagcgg aatatcccgc tctcgcataa aaaactgcgc agggcgctga
     7321 aggccatcac ccgttcagaa agctatctgt gtgccatgaa agccggtgcc tgccggtatg
     7381 acacggaagg gtatgtgacg gagcatattt ctcaggagga ggaagcgtat gcgggagcgc
     7441 gtctggcgaa aattcgtcgc cagaaccgga taaaggcaga acttcaggcc gtgcttgatg
     7501 agaaataaaa aaagcctccc ccagagagag gaggcaggga aataaggctt aaaaggaaat
     7561 aacttccgct tccgaaagga agccagtcca ctgaattatt tatcacaata atactgacaa
     7621 ctaaaggggg acaagaaaat gaatggattc agaaacagtt cacggaacgg ccaggtctgg
     7681 cgttaccagc gtgccggcgg gcgggccgtt attctggaag tcagtggacg ctggatggaa
     7741 gcggcagaag catggcgacg ggctgcctgt gttgcgccta ggacagactg gcagcagttt
     7801 gcccggaaaa gggctgaaca ctgccaccgg cgctgccggg gaagggtgta acgaaaaaag
     7861 cagccttcat tactctagga gtcagaaggc tgaaaggagt ttatgagatt tgcttcttaa
     7921 tatccggtcg ggcagtatga ccggacagtt ctgagtagca cagagtcttt tccgcattaa
     7981 gtcgattttt tatattttgt gattagtttc acaaaataag gtgttattca atgtgtgctg
     8041 cactattcag gggggctgaa atacaggtgg catttatctg atatggagaa taacatgagg
     8101 aaatatattc cactggcatt atttatcttt tcatggccgg tattgtctgc agatattcat
     8161 ggacgggttg ttcgtgtact ggacggtgat accatcgagg tcatggactc actgaaggcg
     8221 gtgcggatca ggctggtaaa tattgatgca ccggaaaaga aacaggatta tggccggtgg
     8281 tcaacagaca tgatgaaatc actggtcgca ggaaaaacgg ttaccgtcac ata
//
LOCUS       DAJTUI010000145         8225 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTUI010000145 DAJTUI010000000
VERSION     DAJTUI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.145"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..306
                     /locus_tag="OO464_003687"
     CDS             <1..306
                     /locus_tag="OO464_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR6228687.1"
                     /translation="LLIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMAL
                     SSTLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            278..2188
                     /locus_tag="OO464_003688"
     CDS             278..2188
                     /locus_tag="OO464_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6228688.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            2189..2392
                     /locus_tag="OO464_003689"
     CDS             2189..2392
                     /locus_tag="OO464_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6228689.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2389..3982
                     /locus_tag="OO464_003690"
                     /pseudo
     CDS             2389..3982
                     /locus_tag="OO464_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3972..5477
                     /locus_tag="OO464_003691"
     CDS             3972..5477
                     /locus_tag="OO464_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR6228690.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5514..5861
                     /locus_tag="OO464_003692"
     CDS             5514..5861
                     /locus_tag="OO464_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR6228691.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5919..6947
                     /locus_tag="OO464_003693"
     CDS             5919..6947
                     /locus_tag="OO464_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR6228692.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6999..7385
                     /locus_tag="OO464_003694"
     CDS             6999..7385
                     /locus_tag="OO464_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6228693.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7397..7774
                     /locus_tag="OO464_003695"
     CDS             7397..7774
                     /locus_tag="OO464_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6228694.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7761..>8225
                     /locus_tag="OO464_003696"
     CDS             7761..>8225
                     /locus_tag="OO464_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6228695.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
BASE COUNT         1906 a         1933 c         2540 g         1846 t
ORIGIN      
        1 ctactcattg ccagatggga actgaccgca gaacaggctg tcgcacagca gttaaaaaat
       61 caggtgtcaa aaggaaatct tatcgatacc ggattttgta tttttgccct tagtaagctg
      121 gcgatggctt tatccagtac gcttgactcc attcctttgt ccatgcaacg acagttcccg
      181 gatttaacgc cgcgtcatat tgatcatctg aaaatcctta ttgctaaggg agcgaatcag
      241 tgcgcgcgtg cgggggataa attaccggat ttactcgatg aatatatcag aacaacaact
      301 gagtaatatg atggtggcag tcactatcgc actgcagcca ctggtcaggg tattgccggt
      361 gacggctgta gaatgggccg atcaaaatta ttacctgcct aaagaatcgt catacggtga
      421 gggggaatgg aaaacgctgc agtttcaggt cgccatcatg aactgcatgg gcaatgacga
      481 gattcgtaca gttaacctga ttaaatctgc ccgtgttggt tatacaaaaa tggtgcttgg
      541 tgtgatcggg tattttattg agcacaaatc ccgcaacact cttctttttc agccgacgga
      601 ctcagctgct gaagatttta tgaagtctca cgtggaagca acaattcgtg atgttccttg
      661 cctgaaaaaa ctttctccct ggctgggccg gaaacaccgg gataatacgc tcacactgaa
      721 gcgtttttcc tctggtgtgg ggttctggtg tttgggtggt gctgctgcca aaaactaccg
      781 tgaaaaatct gtcgatgtgg tctgctatga cgaactctcc tcgtttgaac cggatgtgga
      841 aaaagaaggt tcgccaacac tgcttggcga taaacgtatt gaaggttcgg tatggccaaa
      901 atccatacgc ggttcaacgc caaaaattaa aggctcctgt cagattgaaa aagctgcgaa
      961 tgaatctgcg catttcatgc ggttttatgt cccttgccct cattgcgggg aggagcagta
     1021 tctgaagttt ggcgatgatg cgacgtcgtt tggcctgaaa tgggagaagg gtaaaccgga
     1081 aacggtgtat tacctgtgtg aacataatgg ctgcgtgatc cgtcagtctg aacttgatca
     1141 gaccgggggg cgctggattt gtgacaatac cgggatgtgg acgtgtgacg gtctgacgtt
     1201 tttcagtgct tcgggtaatg aaattccgcc gccgcgctcc atcactttcc atatatggac
     1261 ggcgtacagt ccgttcacca ccagggtaca gatagtctgt gactggctgg atgcactgaa
     1321 agatcccaac ggcgtgaaaa cttttgtgaa caccacgctg ggcgagacct gggaagaggc
     1381 cgtgggcgaa aaactcgatc accaggtact gatggataag gttgtgcctt acacggcaac
     1441 ggtgcctgtc cgggtggttt atctgacggc gggcattgac tcgcagcgaa accgttttga
     1501 gatgtatgtc tgggggtggg ctccgggaga ggaagccttt ctggtggata aaatcatcat
     1561 catgggacgt cctgatgagg aagagacgct gttacgtgtg gatgcggcga tcaataaaaa
     1621 ataccgccat gcagacggta ccgaaatgac catttcccgt gtctgctggg atatcggggg
     1681 gatcgatggc gaaatcgtgt atcagaggtc aaaaaaacac ggtattttcc gggtactgcc
     1741 ggtaaaaggt gcctctgtct atggcaagcc ggtgatcacc atgccgaaaa cccgcaatca
     1801 gcggggtgtg tatctgtgtg aggtggggac ggataccgca aaagaaattc tctatgcccg
     1861 aatgaaggcc gatcccaccc ctgtgaatga ggccacatcg tatgccatcc gctttcctga
     1921 taatccggag attttttcgc agacagaggc acaacaactg gtggcggagg aactggtgga
     1981 aaagtgggaa aaaggaaaga tgcgtctgtt gtgggataac aaaaagcggc gtaacgaagc
     2041 gctggactgc ctggtgtatg cctatgcggc attacgtgtg tccgtgcaac gctggcaact
     2101 tgatctggct gtgcagttgc atcccgcaag gatgcaactg cacaaccgac ccttgaagaa
     2161 ctggcagcga agctgtccgg aggagtgaat ggttacagtc gctgaactgc aggcgctgcg
     2221 tcaggcgcgc cttgatttat taaccggtaa acgggttgtg tcggtacaga aagacggtcg
     2281 caggatcgaa tatacggcag cgtccctgaa tgagcttaac cgtgcgatca acgatgcgga
     2341 gtcggttctg ggtacgaccc gacgccgccg accgctggga gtgaggttat gaaacgaacg
     2401 cctgtcctga ttgatgtgaa cggcgttccg cttcgtgaga gtctcagcta caacggaggc
     2461 ggcgcaggat ttggcgggca aatggcagag tggttaccac cggcgcagag tgccgatgcg
     2521 gccctgttgc ctgcattgcg tctggggaat gcccgggcag atgatctggt gcgcaataac
     2581 ggtatcgcgg ctaatgcggt ggccctgcat aaggatcata tcgtcgggca catgtttctt
     2641 atcagctacc gtctgaactg gcgttggctg gggatgcggg agaccacggc aaaaagtttt
     2701 gtcggggagg tggaagcagc ctggtcggaa tacgcagaag gaatgtttgg cgagatcgac
     2761 gtggaaggga aacgcacgtt tacggaattt atccgtgaag gggtgggcgt gcatgccttt
     2821 aacggcgaaa tctttgtgca gccggtctgg gatacggaaa ccacgcagtt attccgtacg
     2881 cgttttaaag ccgtgagtcc gaaacgggtg gacacgccag gacacggtat ggggaaccac
     2941 tttctgcggg ccggggttga ggtcgatcgg tatgggcggg cgattgcgta ccatatctgt
     3001 gaggatgatt ttccgttctc tggtgccgga cgctgggagc ggatcccgcg tgagcttccc
     3061 accgggcgtc cggccatgct gcatattttt gagccggtgg aggacgggca gacccgtggg
     3121 gctaatcagt tttacagcgt aatggagcgg atgaagatgc tggactccct gcaggccaca
     3181 cagcttcagt cggccattgt gaaggcgatg tatgcagcca caatcgaaag tgaacttgat
     3241 accgaaaagg catttgaata tatcgcgggg gcaccgcaag gtcagcagga taatccgctt
     3301 attaatattc tggagaagtt cacccgctgg tatgacacga ataacgtgac gctgggcggt
     3361 gttaaaattc cgcacctttt ttcctggcga tgcgctgaac ctgcagacag cgcaggattc
     3421 agacaatgga ttttcggcgc ttgaacaggc gctgctgcgg tatattgcag ccggtcttgg
     3481 cgtttcctac gaacagttgt cccgtgatta ctcgaaggtc agttactcaa gtgcccgcgc
     3541 ctccgccaat gagtcgtggc gctattttat ggggcggcga aaatttattg cggcccggct
     3601 ggccacgcag atgttttcct gctggctgga agaggcgctt cttcggggga ttattcgtcc
     3661 gccacgggcg cgttttgatt tttaccaggc gcgttcagcc tggtcacggg cagagtggat
     3721 tagctccgga agaatggcca ttgacgggct caaggaggtc caggaatcgg tgatgcgcat
     3781 tgaggccgga ctgagcacgt atgagaaaga actggcactg atgggcgaag attatcagga
     3841 cattttccgc cagcaggtca gggaatccgc agagcgcgaa aaagccggac tctcacgtcc
     3901 ggtgtggata gcccaggcgt atcagcagca gatagcggag agtcgcaggc cggaagagga
     3961 gacaacatca cgtgagacgt aatctttcac atattatcgc tgcggcattc aatgagccgc
     4021 tgcttctgga gcccgcctat gcgcgggttt tcttttgcgc gctcgggcgt gagatgggcg
     4081 cagcaagcct ttcggtaccg caacagcagg tacagcttga cgctcccggg atgctggctg
     4141 aaacggacga gtacatggcc ggaggcaaac gacctgcccg tgtttaccgg gtggtgaacg
     4201 gtattgctgt actgccggtg accggcacgc tggtacaccg gctgggtggc atgcggccat
     4261 tttccggaat gactggctat gacggcattg tcgcctgtct tcagcaggca atggcggaca
     4321 ctcaggtacg gggcgtactg ctggacattg acagttctgg cgggcaggcc accggcgcgt
     4381 ttgactgcgc tgacatgatt taccgtctcc ggcagctgaa gcctgtctgg gcactgtgca
     4441 atgacacggc ctgttcagcc gccatgttac tggcatcggc ctgctcccga cggctggtta
     4501 cccagacatc ccgtatcggc tccattgggg tgatgatggg ccatgtcagc tatgccggtc
     4561 atctggcaca ggccggtgtg gatatcacgc tgatttacgc cggatcgcac aaggtggatg
     4621 gcaatcagtt tgaatccctg ccagcaaagg ttcgccagga catgcagcag cgcattgatg
     4681 cggcgcgccg gatgtttgct gaaaaagtgg cgatgtatac cggtttatct gtggatgcgg
     4741 ttatgggaac agaggccgcc gtttttgaag gtcagtccgg tattgaggcc gaactggcgg
     4801 atgaattaat caatgcgtcg gacgccatca gcgtgatggc tgcgacgctg aacacacacg
     4861 atacaggagg cactatgccg caattaactg caacggaggc tgccgcgcag gagaaccagc
     4921 gagtgatggg gatcctgacg tgtcaggaag cgaaaggacg tgaacaactg gccacgatgc
     4981 tggcagggca acagggtatg agcgttgagc aggcccgggc gattctggcc gtggcggcac
     5041 cgcaacaatc tggtgtatct gcgcagagtg aagccgatcg cattatggca tgtgaagagg
     5101 cgaaaggacg tgaacaactg gcggcaacgc tggcggcgat gccggagatg acggtggaaa
     5161 aagcccgtcc gattctggct gcttcaccgc aggcgaatgc cggaccctca cttcgtgatc
     5221 agattatggc cctggatgag gcaaaagggg cagaggcgca ggctgaaaaa ctggcggcct
     5281 gtccggggat gaccgtggag aatgcccggg ctgtactggc ggcagcctca ggcagggcag
     5341 aaccggtttc tgcatccaca accgccatgt ttgaacattt catggcgaac cattcaccgg
     5401 cagcggtaca gggtggcgtg ccacagacgt cagcagacgg tgatgcggac gtgaaaatgc
     5461 tcatggccat gccatgaagt cagtgctgac catcaatatg aggtttttac aatatggtaa
     5521 cgaaaaccat cactgaacag cgtgcggaag tacgtatttt tgccggtaat gatccggccc
     5581 ataccgccac aggctgcagc gggatttcct cggcaacacc ggctctgacg cccctgatgc
     5641 tggatgagga taccgggaaa ctggtggtct gggacggaca gaaggcaggc catgtggttg
     5701 gcatactggt actgccgctt gaaggtacag agacggtgct gacgtattac aagtcgggga
     5761 cctttgcgac ggaggccatc cactggcctg agagagtgga tacacacaaa aaggcgaatg
     5821 cctttgccgg cagtgccctg agtcacgcag cgctgccgta acattttatc aggtcactgc
     5881 ggtagcctga ctgatttctg aatgaaagga actgatttat gggattgttt acgacccgcc
     5941 agttactcgg ttataccgaa caaaaagtta aatttcgtgc gctgtttctg gagctgtttt
     6001 tccgccgtac ggtgaatttc cataccgaag aggtaatgct ggacaaaatt accggaaaaa
     6061 cgccggtggc ggcctatgtc tccccggttg ttgaaggaaa ggtgttgcgt caccgtggcg
     6121 gtgaaactcg tgtactgcgc ccgggctacg ttaagccgaa acacgaattt aattaccagc
     6181 aggcggtgga gcgtcttccc ggtgaagatc cggcccagct gaacggcccg gcttaccgtc
     6241 gcctgcgtat catcaccgat aacctcaaac aggaagagca cgccattgtc caggtggaag
     6301 agatgcaggc ggtgaatgcc gtgctgtatg gcaaatacac catggaagga gagcagtttg
     6361 aaactgttga ggtcgatttt ggccggtcgg tgggaaataa cattacgcag gcaggaggga
     6421 cggagtggag cgctcaggac cgcgatacct ttgatccgac gcatgatctg gatgcgtact
     6481 gtgattttgc ttccggcacc atcaatattg ccattatgga tggtaaggtc tggcgtctgc
     6541 tgaatggctt taagctgttc cgcgaaaaac tggatacccg tcgtggctca aattcacagc
     6601 tggaaacggc agtgaaagac ctgggggcgg tggtgtcctt caaagggtat tatggtgatc
     6661 tggcaatcgt ggtggcaaaa acgtcctatg tggcagagga cggtaccgaa aaacgttatc
     6721 tgccggacgg cacactggta ctgggaaata cggccgcaga gggtattcgt tgctacggtg
     6781 ccattcagga tgcgcaggca ttgtctgaag gcgtggtggc gtcttcccgt tatccgaaac
     6841 actggctgac cgtgggtgat ccggcgcgtg aattcaccat gacgcagtcc gcgccgctga
     6901 tggtgctgcc ggatccggat gagtttgtgg tggtgcaggt gaaataatcc gtgagcggga
     6961 gctaaatgct cccgtttttc attcaatcag gagcgggtat ggctacgaaa gaagaaaacc
     7021 tgaaacgtct ttgtgagctg gctgagaggc tggggcgtga accggatgtc tccggtagtg
     7081 cggcagatat tgcgcagcga gtggcggaac tggaggaaga gcttggagat gcgggggagg
     7141 tcgcagagcc ggatacgtac tcaccatcga aagatgatcc gaccgcgcat aaaggcgagt
     7201 cagttcctga acagccaggc attgttaccg gagacgaaac ggggcaggtt acggtggtgg
     7261 cactggcgac actgcataca aaatgtaatg gttcagtgat atttgtttca ccgggaacgt
     7321 cgtttcgtgt ttctgctgag gtggctgcca gcatggcggc aggtggtctg gcaaaaaggc
     7381 agtaacagga gatgtcgtgg acggtttcga taatctgttt gatgctgcgc ttgccggtgt
     7441 caatgaagtc attctccgtg atatggggat cagcgccgtc attacgtccg gagaacttga
     7501 ggggacacac ctgacagggg tttttgatga tcccgaaagt atttcttttg ttgccggggg
     7561 gatccggctt gaggattcct caccgtgcct gtttgtgaaa acggcagata tatcgcagtt
     7621 acgccgtcag gatacgttaa ctatcgggga tgattctttc tttgtggatc gtattactcc
     7681 ggatgacggg ggatgctgtt atatccggtt aaggcggggc agtcctgccc cacagaataa
     7741 tgccagaatg aggcgatatg atgaagggac ttgagaatgc catccgcaat ctgaacagcc
     7801 tcgatacccg catggtgcca cagaccagcg catgggcggt aaaccgtgtg gcggcaaaga
     7861 ttgtttctgt cgccacacgg caggttgcgc agaataccgt tgccggggat aaccaggtga
     7921 aaggtattcc cctgaaaacg gtcagggagc gtgtcaggct gcttaaagcc agcccctcag
     7981 gaaaaatgta tgcccgcatg cgtgttaacc ggggtaacct gcccgccatt aagctgggta
     8041 ccgcacaggt cagactggcc cgctcccggc atggcagcaa ctcacgtcat cgtggcagcg
     8101 tactgaaagt ggggaaatac cttttccggg atgcgtttat tcagcaactg gcgaatggtc
     8161 gctggcatgt gatgcggcgt attgacggca aaaatcgtta ccccattgat gtggtgaaaa
     8221 tcccg
//
LOCUS       DAJTUI010000146         8058 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTUI010000146 DAJTUI010000000
VERSION     DAJTUI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.146"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..436
                     /locus_tag="OO464_003697"
     CDS             <1..436
                     /locus_tag="OO464_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228696.1"
                     /translation="LLLSRKNPFGDIKSDGTVKTALENLGLGEAAKRNVGTGANQIPD
                     MSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPMTFSRVIAVVVTLNV
                     TGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            408..842
                     /locus_tag="OO464_003698"
     CDS             408..842
                     /locus_tag="OO464_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6228697.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1615..2664)
                     /locus_tag="OO464_003699"
     CDS             complement(1615..2664)
                     /locus_tag="OO464_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6228698.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINTINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3115..4305
                     /locus_tag="OO464_003700"
     CDS             3115..4305
                     /locus_tag="OO464_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCR6228699.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4695..6155)
                     /gene="gtrII"
                     /locus_tag="OO464_003701"
     CDS             complement(4695..6155)
                     /gene="gtrII"
                     /locus_tag="OO464_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCR6228700.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6152..7081)
                     /locus_tag="OO464_003702"
     CDS             complement(6152..7081)
                     /locus_tag="OO464_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6228701.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(7078..7440)
                     /locus_tag="OO464_003703"
     CDS             complement(7078..7440)
                     /locus_tag="OO464_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR6228702.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            complement(7702..>8058)
                     /locus_tag="OO464_003704"
     CDS             complement(7702..>8058)
                     /locus_tag="OO464_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6228703.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
BASE COUNT         2479 a         1540 c         1617 g         2422 t
ORIGIN      
        1 gctgctgtta agccgcaaga atccgtttgg cgatatcaaa tcggatggca cggtgaaaac
       61 ggctctcgaa aaccttggtt tgggagaagc agctaaaagg aatgtaggta caggggcgaa
      121 tcagatacct gatatgagcc tgttcgcgtc aattaatacc gtaacggctg ctgcgcaaaa
      181 atttccgtct ggattaattt tacagtgcgg tcagttgaat ggtgccccga atgtatcttc
      241 aacatacggg atgaggttcc cgatgacgtt ctcaagagta attgctgtcg tagttacatt
      301 gaacgttact ggcgcggctg ggcagccgac tgtatcggcg acaagtgtcc agaacactgg
      361 atttaatatt actgtgtcgc ccggttcagg atacggttca tctgctgatg cgtattacat
      421 tgcaatggga tattaacaaa atgtcatatt tttattctgc atcgacaaac ggattttatt
      481 cgactgaatt tcacggcacc aatattcctg atgatgcagt ggaaatctcg gaatcagagt
      541 ggaaaacact gattaatgca cagagtgtaa caaaaatgat tacctgtggt gagaacggtc
      601 atcctgtcat tgttgaccgt ccttctccaa caccagaaca attagcctta ataaatgatg
      661 aaaagaaatc tgcactgata gcagaggcaa cgaatgtaat agctccgctt caggatgcgg
      721 ttgatttagg tatggcaaca gatgatgaaa cgaaactgtt actggcatgg aaaaaatatc
      781 gggtgctgct tatgcgtgtt aatgtagtaa aacccgagtg gcctatgcat ccaaataaat
      841 agacttgtta atctaggata attgttggaa taatactcgt ttttcagtag gttcaggtag
      901 cctgggtata atgctcactt ctacaatgat ttcaacttat tttatttaat tgatattacg
      961 aaacttaact catacataaa caatatgact caatactgtc ctaaatagct gcgcagaaaa
     1021 gtgctccact ttcgctcagc ccttgctggg cgaagcatca ggaaggcgtt ctcagctagg
     1081 ttgtgttata tctggcttga gttttgttgc ccatacgcaa cacattaccc atctgtaaac
     1141 gacaagtgtg gcagaattag gtgcagctat ttagactata tcgacgcagt aacgataata
     1201 gatatcgaaa ctgtactaga tattagctac ttatggttac cggggggagt cttttgagca
     1261 ctggtgttat caattggctt caaataattc gccaataaat tatattgcga tagctcatca
     1321 ccttccaggt tgtttatagc cagtttatca gcgtcaccac ctactgttta tgcttttatt
     1381 tctttgatta ataagtatat gatattttaa ttcatctggt gtatttcttt ctcatgtgtt
     1441 ggtcgactga atatctgttc agtgaagggg taattttatg tttgcacttc gaacattccc
     1501 ttcagttgct aggaaagaaa accgtaacat ttgcgcatta attttaacag tataatctga
     1561 gcataaatat gcgctatata tctataaaaa ggcgcaatta attgcgcctt tttatcattt
     1621 tatcaaaaat ggcccaccga ctgtcctcga tagccaatgg ccaattttat ctgatacata
     1681 tacacaaata caggtatata tgattgcgca gataggattt ggaatagtca cgctgaagcc
     1741 tataattgct aaagccatag ggataaataa tttggtgata tactccattc cacatatgtt
     1801 aagagtattt cttccagctc ttacaataaa gtcatttcca caaattattt ttgctatgaa
     1861 aatattaaag attatcaagc ctaccgtggt gataatagta ttaatagctt gaatataact
     1921 acttgatatt atgtctgcat tgaatattga gataatctta aaaagtaaag gtttctggtt
     1981 aagtagttga tacgcactca ttatagcggt aattgagaag acaatgaaac caattttaga
     2041 ttttccaaaa aacctgtcac gtgttaattc taaaaacata catctaccga gaggcaataa
     2101 ccaccagtat gccatagcgg agtctatgtt ccaaaaccat tgaggatcta gtaatgggtt
     2161 atgattgagc actgtttgtg acaacatgaa cgataacagt gatatgatta aaatcactat
     2221 attgttcttg actatttctc taaagattgc atcaattgct attataacaa aaaggcagtt
     2281 tataaaccat attgttccta cgaattggtt atttctaaca ccatatatta tatcataaat
     2341 gtggttgtat atataatcaa tcgtctcacc tgagttaatt gtgtttatta ttaatataga
     2401 aaatgcaaaa gtgaaatatg gaatcattaa tctgtaaaac tttgacttga tataatcgaa
     2461 aacagataag tcattttttt tgatagtaaa aaaaccagca gcgaaaaaga atagtgggac
     2521 gtgataacta aaaacaaaag gatacaattt gccagccccc aacccaaggt gacctaaata
     2581 tatagcaaaa ataccgagaa attttaaagc atcaacccaa tcatgtctta tttttctgtt
     2641 gctgtcgatt ttgttattat acatttaaag taggctctcc agttttgtgt gatttttaaa
     2701 cactcattgc aaatatgaat gcctgaaagt ttatgattat taatcaacga gttcaatatt
     2761 aatttattat ttttcaaagt cgcactatat aggcatttac ttccgcagta ccaccgatag
     2821 tatcgatatc aaatcagcct gcggccgcga taactttcgt aacattctgc cagcggcttt
     2881 gttgaataaa tcaggttcaa gataatcgtc tctgcatcag gttagagttt caggcaatac
     2941 atcctctttc tggcatacct gccactctcg caccatggtc atagccttta cgatctgcat
     3001 actcaccaga ccagagcccg ctccctttta ggctgaggaa tccccagaaa agaagacagg
     3061 catagagttt tgtgatctac tgattgcgct aacaaattca atgagatcac ctctatgcct
     3121 gcccgtaaag tatgccagac ttttttccgt aatgctttag cttccacgca tcagtatcga
     3181 caaaatgcca ttattgattc tgctgctgct ttagtcggtg gcgcatctct ttctcttacc
     3241 agcattggcc ggcatttacc cggtcctgct cgcgtgaaag ataaaataaa acgcgttgac
     3301 tgtctgttgg gtaataaacg acttcataat gatatccctc tgatattcaa aaatattaca
     3361 tccatgatga caaataaact gtcgtggtgt gttatcgctg ttgactggag cggttaccct
     3421 tttcaggaat atcatgtcct tcgtgccagc ctcctctgcg acggtcgctc tatccccctg
     3481 atgagtcagg tcttcccgtc aaaaaaaaat aacgaggcgg tggaaatcgc ttttctggat
     3541 gcgctttccg gggccatttc tccccggact cgtgttgtta ttgttactga tgctggtttt
     3601 caaagtgcat ggttccgtca tatcaaatca cagggatggg attttattgg tcgtatcagg
     3661 ggggtagtga agttcaggct ggatagtgac aaagataagt ggctggacat aaaaatgtgc
     3721 agggggagtt cagaggcaaa atacctgggg acgggaaccc tggcccggaa aaaacgctcg
     3781 cagtgtgaag gacattttta tctttataaa cattcaccta aaggcagaaa aagcaggcgt
     3841 gccagaggga ggccgggctt accaacaacg gaaaaagaac aaaaagccgc aggcagagaa
     3901 ccatggttaa tattcagcaa tacagctgag tttaatgcca aaaaaatcat gaaactgtac
     3961 agtcgaagaa tgcagataga gcaaaacttt cgggatgaaa aaagtgaacg ctttggtttt
     4021 ggccttcggg cgagcagaag tcggagggga gagcgttttc tggtattaag tctactggtt
     4081 acactggcca gtatcgtact ctggctactt ggttatcatt ttgaaaataa aggatttcat
     4141 ttaaagtatc aggctaatag tctcaaaaaa cgaagagtat tgtcttttct gacgctggct
     4201 gaaaacgtcc tgagatttaa ccctgaactt ctacgacgag cacaacctga aaagatattg
     4261 tgccggttgg ccagcactta ccgaagtatg gtgctggctt actgatgaaa attttctggg
     4321 gatccctcag cctttcgggg aggaatgagg gctctgatat tcttttgccg tagttcatcg
     4381 tgaaaccgtc ggatgtcatt tggcctgagt gacccatgcc tcccaaaagc agagtcgcta
     4441 gtgttatgta attaggcggt taaacttcaa ttctcagtgt taagtttggg taaacgcttg
     4501 taaatggtat tgctaatttt ttaattgact aatattagaa acggttagtg tgattgtata
     4561 tagatatttg agtgtaaggt atttaaaggg gcattcagcc cctccgtcta tttgtgattg
     4621 catccattga tgtgtgtatt attttgagct tgaaaaaggg aggcgctctt taataataaa
     4681 taaatctatc aaagctaaat attaaatgga agccaccctt ttttctctga tagttttatt
     4741 atcaccaaac catttatttc agctatcgaa tctggattcg gccaggaagg taaactcttc
     4801 acaattggat aaatcttctc tatttgttct ttatttgctg gtttgcaatt acagattcca
     4861 tagtaattca taaatgcaac catcctaaaa tagttgccac catcccacca aaaaaaagat
     4921 tttccaaaag tgtctgcgct catggagagc atatttcctg agtcgtttga accatatata
     4981 tatatatatt ttccgttaag ggaaatgcct tttgtttgag atgtgtatga tatttcttta
     5041 gctaaagaga tatctttctg tcttgcgaga gtatcattca aaaatagatt tttggatgtt
     5101 gaaacgccat taaatataat aaataaaaaa aacatgcagt ttaggtattt tatagagcga
     5161 aaattgctta agcaagaaaa caaaattact gctactatag gcattaaaac aaacaggcga
     5221 ggtggcgccc ctgagccaac aacaacgata aatataaccg gaagtatata aatgaaaatg
     5281 attgatgaaa tcaaataaat tgatcttgtt tttaatttat atgttagggt aaacatcaga
     5341 attaataaag gaatcagtaa ccacttgtat aggtttaaac cagtgggagg attgttatag
     5401 aagttatata tattatctat ggctgattta acaagccatt taatatcgct tgcattttgt
     5461 atataatttg agatgtactc gttcgattca agcgaaaaat aatgcttgat agctttggtt
     5521 aatagcaggt aaattaaggt agataaagct atgagggata gagataaaca tgaactataa
     5581 aaagattcac gaatattttt ctcatttcgt attatcgaat ttatctgcaa cccaatgacg
     5641 aatgcaataa tatatgttac gaatgtttgg tagattgcca ttgaaagaat ggacagtact
     5701 ataccagaaa atatcacaat cctatttttt tgcgagtgaa aaataattgc tgatatcgct
     5761 gccagtagaa aagcaattcc cacagtatca gcttggttaa gaaactctaa ttgataggag
     5821 atctgaggga aggtaataaa tactaacata cctatcaata attcataaga ttctagcttt
     5881 agcgatctgc agattataaa tgctgaaatg atgataaaag ataaggctat taatggtgtt
     5941 atataaagtg aaaaaggctc agggagaaag taatgtcgca aaaaagtgtg aaaccaccgc
     6001 ccaagcgtga ttgtttgata aaaattattt gtaaactcgc catcaatatt aagtgttcca
     6061 tcgtacaacc acccccaata acaataaatt gatattgcaa aacatgatat gaaggcaagg
     6121 agatttgcat ttttaaataa attaatttta atcatgggtt acctcgatga gattttttta
     6181 tgatgtattt gggtctattt tttacttcaa tatagattct acctatatat tctccgagaa
     6241 caccaatccc gatcagttgc actccaccca agaaaagtat tgatacaagc agggagggat
     6301 acccgcgtac tgggttacca aagacaaggg tgtctataat catccatgca ccatataaaa
     6361 atgaaatgct tgcaacaaac aagcctatat aagtccatac gcgaagagga aaggttgaaa
     6421 aacttgtgat accttccagt gccagattcc ataatttcca gccattaaat tttgagatgc
     6481 cagcaacacg ctctgcgcgt acatattcaa cgacatccgt cggaccaccc acccagctca
     6541 gtatgccttt catgaaaaga ttgcgctcag gcaacagttt aatgttctcc acaacctcac
     6601 gagacatgag tcgaaaatct ccgacatttt cctcgatctt tggggtgctt attttgttgt
     6661 gtaatttata gaaccactca gctgtcttac gtttcagtcg tccatcagtt gagcggtctg
     6721 aacgtttagc aagcaccatg tcagcacctg cctgccactt ttcaataaga tgagggataa
     6781 cctcaattgg gtcttgcagg tcgacatcaa taggaattac agcatcgccg cttgcatggt
     6841 ctaaccctgc aaataaggct ggttctttac caaagttgcg tgtaaatgac agcggaacaa
     6901 ctagcgggtc tgaaacagcc agcgcgttaa taattgactc tgtggcatct ttacttccgt
     6961 catttatgaa tacaatttct acttcatatg gcttcaactc ttggaattca cgtaccgttt
     7021 tatagaaaac aggtatcgct tcttcttcat tgaagacagg aacgacaaga gatattttca
     7081 tttcgcatcc ctaaagacaa tgaactttga atagacgaaa ccgcacacca gactgatggc
     7141 ggagaaggtg ataagagtga ctattggagg aagtgagcat ttatcagctg cccaaccaac
     7201 aatcacactc aatattccca taaatcccac gtataacatg tagcgcatcg ctgtagtcaa
     7261 tgctttgaat gtgaatcttg cattcgcgaa gaagctaaag ctcacagcca ctacgaaacc
     7321 tgtgaagttt gccagagcct gactggtatg tgcggcatag atacatacac caaaaaccac
     7381 ccagtgtata agtgtgttca gcacacctat agatgtgtac tttacaaata actttaacat
     7441 ttatttaatc aatgagctct gaaaggcatg aagtctatca tccaagtctc aattgatcga
     7501 tacttgctat gtctgatgag acaaaactga gacacataag gcctcacaat ggcttgcaag
     7561 gctttacatg ttttgatgtg gtgggacgtg tgagcgcagt gttgatggga taatcctttg
     7621 aattacaagc ggattcttat aattcgtaat gcgaaggtcg taggttcgac tcctattatc
     7681 ggcaccattt aaatcaataa gttacctcgc atttaagtaa actacgttct cctcttgtgc
     7741 cgtatttgtg ccattgcgac ttataatcgc atcgattttg ctcgcgtgct cggtaagatg
     7801 cccggctgaa aggtgggcgt atctttgaac catttcgaga gtttcccatc ctcccatctc
     7861 tttaagcgca agaagagaga caccggactg aaccagccag cttgcccagg tatgcctcag
     7921 gtcatggaag cggaagttgc taatgcctgc ccgctttaac gctcccttcc atgccttgtt
     7981 gctgtcggtt ctcatcttcc ttaccgctgc tgtttttgtt ccgtcgcttc ggtaggcagg
     8041 tttggtgtgg acaaacac
//
LOCUS       DAJTUI010000147         7990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTUI010000147 DAJTUI010000000
VERSION     DAJTUI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.147"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..106)
                     /locus_tag="OO464_003705"
     CDS             complement(<1..106)
                     /locus_tag="OO464_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR6228704.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(147..602)
                     /gene="csgB"
                     /locus_tag="OO464_003706"
     CDS             complement(147..602)
                     /gene="csgB"
                     /locus_tag="OO464_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR6228705.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            1356..2006
                     /gene="csgD"
                     /locus_tag="OO464_003707"
     CDS             1356..2006
                     /gene="csgD"
                     /locus_tag="OO464_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6228706.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2011..2400
                     /gene="csgE"
                     /locus_tag="OO464_003708"
     CDS             2011..2400
                     /gene="csgE"
                     /locus_tag="OO464_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR6228707.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2425..2841
                     /gene="csgF"
                     /locus_tag="OO464_003709"
     CDS             2425..2841
                     /gene="csgF"
                     /locus_tag="OO464_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR6228708.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2868..3574
                     /locus_tag="OO464_003710"
                     /pseudo
     CDS             2868..3574
                     /locus_tag="OO464_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3707..4198)
                     /locus_tag="OO464_003711"
     CDS             complement(3707..4198)
                     /locus_tag="OO464_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6228709.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4300..4854)
                     /gene="ycdY"
                     /locus_tag="OO464_003712"
     CDS             complement(4300..4854)
                     /gene="ycdY"
                     /locus_tag="OO464_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6228710.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4878..5615)
                     /gene="ycdX"
                     /locus_tag="OO464_003713"
     CDS             complement(4878..5615)
                     /gene="ycdX"
                     /locus_tag="OO464_003713"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6228711.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="OO464_003714"
                     /pseudo
     CDS             complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="OO464_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6843..6930
                     /locus_tag="OO464_003715"
     tRNA            6843..6930
                     /locus_tag="OO464_003715"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6877..6879,aa:Ser,seq:gga)
     gene            complement(7080..7361)
                     /locus_tag="OO464_003716"
                     /pseudo
     CDS             complement(7080..7361)
                     /locus_tag="OO464_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7643..>7990)
                     /locus_tag="OO464_003717"
     CDS             complement(7643..>7990)
                     /locus_tag="OO464_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6228712.1"
                     /translation="PITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLG
                     ISKRGDKNLRRLLVQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARI
                     AWAITTQKKEYQA"
BASE COUNT         2168 a         1919 c         1737 g         2166 t
ORIGIN      
        1 cgccaccacc gtggttaccg ccgccgccgt actgaggaac aacacctgcc agagcgctac
       61 cggagaatac gattgctgca attgctgcta cttttaaaag tttcatgtaa aacccccatc
      121 ggattgattt aaaagtcgaa tggtaattaa cgttgtgtca cgcgaattgc catttgcgac
      181 tgtctctgca ctacaactgc cgttttttga gtaccatact gtgtaatatt tgctttatta
      241 ccagaacctt tctggataat catcgcagta ttaccataag caccttgcga aatactggca
      301 tcgttggcac tgcccgcctg atcaatatat gcaaggttat aatctcctgt ctggtcaatc
      361 tttgcccgat tgctactacc ttcttgcgca acaaccgcca aaagttttga gcctccctgc
      421 cgtaactgag cactattatt agtcccagcc tgaccaatta tggctgcctg attaaatgaa
      481 gacttactca attcatttac cgcgaagtta tattctgaat tagctaaatc ataacctgct
      541 gcggctgcaa tcccaggcgc acccagtatt gttaacatca taaataacaa tttgtttttc
      601 atgttgtcac cctggacctg gtcgtacatt taagaaatta aatcatttca acttggttgt
      661 taacgcaacc tgtattttgt taacgctgcg ttacgatgga aagtatgtct gcggaaatat
      721 ttttaataac tcacccgcgc gttgtatttt tctttttttg ttcataccaa agtattaaaa
      781 cccgcgtaaa tacgctgtta tctacgcaaa aaatattttg ttttctctta aatctccgtt
      841 ttccgctatc aaaaatcacc agacagtcat tcttcttgcc cgtcgctgat tgctgcgata
      901 tgtcttgcgc acaagccgtg acaaacctcg tctttttgca gtaaaaaaat tgtccacgga
      961 ggtgtggaga aaaaacaaaa acgttttaca tgacgaaagg actacaccga aatatttttt
     1021 atatgtatta ttagtaagtt agcaccattt gtatgatttt ttaaaattgt gcaataaaaa
     1081 ccaaatgtac aacttttcta tcatttctaa acttaataaa accttaatgt taacatttta
     1141 atataatgag ttacattttg ttacatgttt aacacttgat ttaagatttg taatggctag
     1201 attgaaatca gatgtaatcc attagtttta tattttaccc atttagggct gatttattac
     1261 tacacacagc agtgcaacat ctgtcagtac ttctggtgct tctattttag aggcagctgt
     1321 caggtgtgcg atcaataaaa aagtggggtt tcatcatgtt taatgaagtc catagtattc
     1381 atggtcatac attattgttg atcactaaac cttctttgca ggcgacagct ctcttgcagc
     1441 accttaaaca atcgctagca attacaggaa aattacataa tattcaacgt tctctggacg
     1501 atatctcttc aggctctatt attcttctgg atatgatgga agcggataaa aagcttatcc
     1561 attattggca ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata
     1621 cgcctgaaga ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt
     1681 atgccatgga ggatcaagaa cgtgttgtca atgggttgca aggcgtcctg cgtggcgaat
     1741 gctactttac gcaaaagctt gccagttacc tgattacaca ttctggtaac tatcgttata
     1801 acagcacgga atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta
     1861 tcggcgcgtc taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa
     1921 cgcatcttta taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct
     1981 gggcaaacga taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg
     2041 gcagaatttc tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg
     2101 ttaactgacc atactgtttc atctattggc catgattttt accgagcctt tagtgataaa
     2161 tgggaaagtg actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga
     2221 agctggatca caataacggt caatcaggat gttattttcc agactttttt attcccgttg
     2281 aaaagagact ccgagaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat
     2341 cgtcgccaga taaatcaggc gttattaagt acggacgatt tggcgcatga tgaattctaa
     2401 ataaaaaatt gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt
     2461 atttcgccat taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt
     2521 ggtaacccaa ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa
     2581 gatccgagct ataacgatga ctttggtatt gaaacaccct cagcgttaga taactttact
     2641 caggccatcc agtcacaaat tttaggtggg ctactgtcga atattaatac cggtaaaccg
     2701 ggccgcatgg tgaccaacga ttatattgtt gatattgcta accgcgatgg tcaattgcag
     2761 ttgaacgtga cagatcgtaa aaccggacaa acctcgacca tccaggtttc gggtttacaa
     2821 aataactcaa ccgattttta agccccagct tcataaggaa aataatcatg cagcgcttat
     2881 ttcttttggt tgccgtcatg ttactgagcg gatgcttaac cgccccgcct aaagaagccg
     2941 ccagaccgac attaatgcct cgtgctcaga gctacaaaga tttgacccat ctgccagcgc
     3001 cgacgggtaa gatctttgtt tcggtataca acattcagga cgaaaccggg caatttaaac
     3061 cctacccggc aagtaacttc tccactgctg ttccgcaaag cgccagggct tacaaaacct
     3121 gcttaacgag cgcaaggtta ttcgtgcggc acaagaaaac ggcacggttg ccattaataa
     3181 ccgaatcccg ctgcaatctt taacggcggc aaatattatg gttgaaggtt cgattatcgg
     3241 ttatgaaagc aacgttaaat cgggcggtgt tggggcaaga tattttggga tcggtgcaga
     3301 cacgcaatac cagctcgatc agattgccgt gaacctgcgt gtcgtcaatg tgagtaccgg
     3361 tgagatcctc tcctcggtga acaccagtaa gacgatactt tcctatgaag ttcaggccgg
     3421 ggttttccgc tttattgact accagcgctt gctggaaggg gaagtgggtt acacctcaaa
     3481 cgaacccgtt atgctgtgtc tgatgtcggc tatcgaaaca ggggtcattt tcctgattaa
     3541 tgatggtatc gaccgtggtc tgtgggattt gtaaaataaa gcagaacggc agaatgacat
     3601 tctggtgaaa taccgccata tgtcggttcc accggaatcc tgacagataa aaatgccgga
     3661 attaataatc cggcatttac tgtttaagcg aaagcgtgag tgcccctcac gctttatttt
     3721 tgatttcctg ctcacggtga gccgtctttg cactacgcgc cgccagccac aggccgctgt
     3781 ttttcattgc gtaaccaaat accagcccaa gcgccagcga aggtaacacc agtttccagt
     3841 caccctgccc ggcaaacgtc gcgcatgcgc ctataaacgt ccccggaaca aatgaaagca
     3901 gcagctgttt ggcctgaata cacatcagaa acgcgacaat accggttatg acataaccga
     3961 gaatttccag atgtggtgcc agtgcactac cgtaaataat gaccatcgcc cacaccacgc
     4021 cactaagcag ggttgcagcg gagatcgcca gccctttcag cccaccttgc gggcaagcaa
     4081 agtaggccgt acagcccagg aagcccgccc agcttagtaa gcccagggaa acagccaccc
     4141 atccccagat accggagaga atgcctgttg tgattgcaat agagagaagt atgttcatgc
     4201 cgcgcatgat agcagaaaac gcggccatga atgagatcga gcacacattt tatgcaacaa
     4261 caaattatct gttgcagtta tgagtgacga tgatcacatt tactcttcag aatcttcttc
     4321 cagctcgtcc cacattgcac taatggcatc acgggttagc ggtgccatgg tgcgccagaa
     4381 aggcgtggtt gcatgggcct ccactttgcc gaggaacgca ccacaccagg gtaacagata
     4441 ctcactgaac agtgtttcca gtgcttcgct ctcatcttcc gttgactgat cttccagcca
     4501 ggaagccgca agcagcaatg tgccgatgtg atcggctggc gtatccgcca gtagcatccc
     4561 tcgttcggaa agaaaagcgc gcacttccgc ttccgttgcg tcctcaaccc atgcgctacg
     4621 atatggcggc acggcacatt catcgccgat aaacaacgca ttgtaatcgg cagagacttg
     4681 cgtcatatca cagcttttct gtaaacgcgt cagtaactca tcctgctcca gtggccagtt
     4741 cgcagccagt ttcccctcac gaatcagagt aaacagcggc accagtaaag gatcttgcgg
     4801 ttggcggtaa tagagcgaac ccagcacacg acagaggata gaaaactcgt tcattaatat
     4861 atttccatta ctagtaatta aaggtctgca aattccgcaa tcggtgccat accgcgagat
     4921 tcaaggaagt tcagtaagcg gcgcggagaa acattcaaaa tgcgctctag cggaaaatct
     4981 accgcgtcga ggattttaag acactcttca aattccccca tggtaaacgc ggtgtgagaa
     5041 tccgagccta atgccaccca acctccagca tcacgtaccg ctgcagctac cgcacgacag
     5101 ttgtcttcac tgcccttacg tgagtgtaaa aatgaggaat tattgatttc cagcgccacc
     5161 tgatgtttcg cggctgcttc agcaacggct ttaacatcta tttcatattt cggatttccg
     5221 ggatggctta ttatatgcac attgccgctg gcgatagtgg agatcatcgc ttgtgtattg
     5281 gtcgctttgt catgtggcgc aaaaaccggc tcatgaaaac cggcaataat cagatccagc
     5341 gagtcaaaca ttttaccgct gcagtcaatt tcaccatcaa catttttaat gttagcttca
     5401 atgccgcgca ggatccctac cccatcaacc actcgcggcc agatacgcat gttaatgaag
     5461 tgccagtgat gcggcgcatc ttccatatcc gggccatgat cggtgatcgc aaaaagttta
     5521 atgccctttt gtttggcctg ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg
     5581 gcaacggtat gcatatgaag gtcgacggga tacataactc tctcctgtat tcaattcccg
     5641 ccaaggatag caggaatcct gatgctttat tagtagccac gtgcgcggtc gacttgcccg
     5701 cagaccctct cccctttttc gagctgggca atagtgcgag aaatgtactc cacagcttca
     5761 gcgggacggg taatcgcggc gacatgtggt gttatcgtca cgcgtggatg ttgccagagc
     5821 ggactttcag gcggtaaggg ttcacgatta aaaacatcca gcattgcgcc tttaactttc
     5881 ccgctatcca gcgccgcgag caggtcatct tccacaacat gaacaccacg cgccaggttg
     5941 aggagatacg cgccatcagg taatttttcg agtaattgtt gattaataat gccgacggtt
     6001 tcaggggtat tcggtaacaa attaatcaat acccgacatt ggctcagaaa tgcagacagt
     6061 tcttcccgtc cggcaaagct ttgcacgcca ggccacgatt tacgggttcg actccagcaa
     6121 cgcagcggaa agcgctaggt ttgcagactc tgagcaactt tacagcccag tacgcctgcg
     6181 cccaaaatgc cgatggtaaa atcttcccga tgatattcag gcagcggttg ccaatgcgaa
     6241 ctattttgct ggatgcgata atcgtcaaaa cgtcgaaacc aatgcagcac ctgactgaca
     6301 gcatattcct gcatttgctc gcccataccg gtatcttcca ggcgaaaaag tggaacagaa
     6361 gggttcagca tttcagggtg tgcctgtagc ttgctcaaaa tagaatcaac accggccccg
     6421 agtgcgaaca ccgctttaag atcgcgccct gccagcattt caacaggagg atgccagact
     6481 aaaacataat cagcagagtc attatctccg cttttccatg ccctgactct tgcctgagga
     6541 atagctttgc gcagtgcctc aatccaccat tgggtatcga acgttgggtg ataaaagatg
     6601 atatccatac tgactcccga aaagcgttgt gcgaaattta ttcgcactta tcgttatgat
     6661 ctacaaaggc caccagcata acaaatccgt ggtcggtggc aaaaaaagca gatttcgctt
     6721 attaaaacca cacattgatt gaatgtttga ataaacgcgc gattttttca aaaagtttgt
     6781 tgacctcagg tcatgatttc cctaaattag cgcccgttcc agcaagacag gaacgacaat
     6841 ttggtgaggt gtccgagtgg ctgaaggagc acgcctggaa agtgtgtata cggcaacgta
     6901 tcgggggttc gaatcccccc ctcaccgcca gattataagt gcatgttatt gtttaacatg
     6961 cgctttttta tttttccttc cagacagtat gccatcgggt atgccatctg aaaccggttt
     7021 atatgccccg cgatcatatc ttatcggaca atttccgatc tttgagcata ctggcaaagc
     7081 tatactgcca gcatatcagg atagtgtttt gcgataatat cattcagtct gtcaaccagt
     7141 tctggttttc tgaacgtgat atgcgcggag cccttcttaa agtatctgat gctgaacatc
     7201 tcatcttcat agctctcttt accctgaaca gactgaatgt gatcatccag acgaagggtg
     7261 atgtttcccc ggttgtcggg gattggttta ccgctgaaca ggtgcaacat tctttccaga
     7321 tcggcgagtc ggtcagcctg ttgtccggtg ataagatgaa atctatggtc acccccgttt
     7381 ttgcaacact gatttttgat taatggttgg cttgcttaaa tctatccggc gtctctgtgg
     7441 gcaaggatgc ccgcgccccg atgagttccg cacctgataa tcctaaaaat cccctcggct
     7501 tcagtgagcc atttcatctg ttaggttttt aatcaggtcg atttgccgtt catgtcatca
     7561 ataatcagtc ttcgcaaaac cagaaaggta acttgtatct tattgtttaa aataaactaa
     7621 ttgagttata tcagcatcta tatcaggcct gatattcttt tttctgagta gtgattgccc
     7681 aggcaattcg agccagttta tttgccagag cgcaggtaac cacattcgaa tgcttcttgt
     7741 tgagttgctc ccgaacccac tcagcaagtt ttccatgctt gcgttcaagt cgcattatga
     7801 atgagcgggc acactgaacc agtaaccggc gtaaattttt atcacctcgc ttactgatac
     7861 ccaacagagt cgattttcct ccagtgctat actgccgggg aacaagtcct gtggaagctg
     7921 caaaatccct gctgcatgag aactgtttcc cgtcaccaag ttgcgacgat agtaagctgg
     7981 cagtaatcgg
//
LOCUS       DAJTUI010000148         7818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTUI010000148 DAJTUI010000000
VERSION     DAJTUI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.148"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..1333
                     /gene="yjeM"
                     /locus_tag="OO464_003718"
                     /pseudo
     CDS             1..1333
                     /gene="yjeM"
                     /locus_tag="OO464_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1484..4795)
                     /gene="mscM"
                     /locus_tag="OO464_003719"
     CDS             complement(1484..4795)
                     /gene="mscM"
                     /locus_tag="OO464_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6228713.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4817..5785)
                     /gene="psd"
                     /locus_tag="OO464_003720"
     CDS             complement(4817..5785)
                     /gene="psd"
                     /locus_tag="OO464_003720"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6228714.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5882..6934)
                     /gene="rsgA"
                     /locus_tag="OO464_003721"
     CDS             complement(5882..6934)
                     /gene="rsgA"
                     /locus_tag="OO464_003721"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6228715.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7029..7574
                     /gene="orn"
                     /locus_tag="OO464_003722"
     CDS             7029..7574
                     /gene="orn"
                     /locus_tag="OO464_003722"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6228716.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1770 a         2027 c         2116 g         1905 t
ORIGIN      
        1 ccttaatgat ggctgaaatg ggagctgctt atcgcaaaga agaaggtggt atctattcct
       61 ggatgaataa tagtgtcgga ccacgttttg ccttcattgg tacgtttatg tggttttcct
      121 cttatatcat ctggatggtg agtacctccg cgaaagtttg ggtaccgttc tcaacattcc
      181 tctatggtag tgacatgacc cagcactggc gtattgccgg actggagcct acgcaggtgg
      241 ttggtctgct ggcagtagca tggatgattc tggtcacagt cgttgcttca aaggggatta
      301 ataaaattgc ccgcattact gcggtgggcg gtattgcagt aatgtgtctg aatttagtat
      361 tgctgttagt aagcattact attttgttat taaatggtgg gcatttcgcg caggatatta
      421 atttccttgc atcaccgaac ccgggttatc agtccggtct ggcaatgcta tcgtttgtgg
      481 tatttgctat ttttgcctat ggcggaattg aagcggttgg tggtctggtc gataaaacgg
      541 aaaatccaga aaagaacttt gccaaaggta ttgtttttgc cgctattgtt atttcaatcg
      601 gttattcgct ggcaatattt ttatggggcg tcagcacaaa ctggcagcag gtattaagta
      661 atggttccgt taaccttggc aatattacct atgtgctgat gaagagcctc ggggtgatgc
      721 tgggtaacgc actgcatttg tcacctgaag cgtcattgtc gctgggcgta tggtttgcgc
      781 gtattaccgg actttcgatg ttcctcgctt ataccggtgc gtttgctatt caccgctgaa
      841 agccatcatc caggggacgc cgaaagcgtt gtggccggaa ccgatgacgc gcctgaatgc
      901 gatggggatg ccgtctatcg ccatgtggat gcagtgcggg ttggttactg tcttcattct
      961 gctggtttcg tttggtggcg gtaccgcatc ggcgttcttt aacaagctga cgctgatggc
     1021 gaacgtgtct atgacgcttc cttacctgtt cctcgcgctg gctttcccgt tttttaaagc
     1081 acgtcaggat ctcgacagac catttgtgat tttcaaaacg cgtatgtcgg caatgattgc
     1141 gacggtggtt gtcgttctgg tggtgacatt tgcgaacgtc ttcaccatta ttcagcctgt
     1201 ggttgaagcc ggagactggg acagcacatt gtggatgatt ggcggccctg tcttcttctc
     1261 gctgttagcg atggcgattt accagaacta ttgcagtcgc atggcgaata aacctgagtt
     1321 agctctcgac tgatcatatg aaatcctgca actcatcgtc tctccggcga tgagttgtca
     1381 ggaagtaatg cgtatggatg atactctcca gggccggtga taactgaaga tgatgttaag
     1441 ctgctaaatg agccacgttg acatcatttg gcagcttcat gcattacaaa cttcccgcct
     1501 gacgaccttt gcccgcagac gtcagcgttc tccccgtttg tttaccgttg aggctttcca
     1561 gacgcatctg gaagggcggg aatggcatat cgataccgtg ggcgtggaag cctgccagaa
     1621 tcagctggtg gatctcatgg cgtagcggca tacggtgacc catctcagcg gcgtaaatac
     1681 gcagctcgaa aatctgaatc ccctgttgca gatccaccag gaagacttcc ggtgccgggt
     1741 tgtcgatcac cagcgagcag cgacgcgctg cggtgagcag gatttccgtc acttcttcac
     1801 tgttggcatc ggcaggggcc ggaatcgtca acactacgcg cgtgaccgag tcagagagcg
     1861 accagttgat aaactgctcg gtaataaacg ccttgttcgg cacgattatc tctttacggt
     1921 cccagtcgct gatggtggtg gcgcgggtgt taatttttgt cacgctacca gtaagatcgc
     1981 ggatcgttac cgtatcgcca atgcgaatcg gtttttcgaa caggatgatc aggccagaga
     2041 taaagttggc gaaaatttcc tgcaaaccaa aaccgagacc aacaccgagc gcggcaacca
     2101 gccactgcaa tttcgaccac tcaataccaa tcattgagaa gccgaccagc ccgccaatca
     2161 gcatcagcag atattttgtg atggtggtga tggcgtaacc cgtacccggc gttaaatcca
     2221 ggtgctgcaa aatcgccagt tccagcagcg cgggcaggtt gcgcaccagc tgcgtggtga
     2281 tgataaatac cagaatggca atcagcaccg caccgagggt aattggctcc agactttcta
     2341 cgccctgtac cgtggaggtg acatcccaca gcgaaatatt ttcgaggaag ccgaaagcgg
     2401 aatggatttc tgaccacagc acaatgaccg aaagcagggc gatcaacatc aaaattgagc
     2461 gcaccagccg caaggattgc gcactgatgg catcgagatc gacttcgctt tcatcgactt
     2521 caattgctcc ttccgggcta ctgtgatgat gcgcttcctc ttcgccacgc gcacgttgcg
     2581 ctaacatctc tgcccggcga tgcttcgccc ggtcaaacgc cagcctgcga cgctggatca
     2641 gcatccagcg gcggataacg tgataaacca ccagtagcag gaaccagatg gcaaccgagg
     2701 tttcaagcct cgctaacagt gcctgcgccg ttgccagata acccaccgcc gacgccagaa
     2761 tggcaaccag tggtgcgcca atcatcatgt tccacagcat atggttggta atgttgtcgc
     2821 cgctgccttc tttgttgaga tacaacggaa taccggcttt tttcaggctt agggtgacca
     2881 ccgccagcgc accgcaaatg aggataaagc aaagccgtcc cagcgaaccg gaaaattcgc
     2941 ggtcgtcgag gttatcgaac atcatcagcg ccataatcag cccgatgctc atcaggtagt
     3001 agcgcatccc acgggaaaca cgttcgcgcg gccagccaaa atgagcgata aacaagccgt
     3061 tcgggcgggc aaaggtggcg caaatcatca ctacccacag cagcggcacg gtggccgtta
     3121 caccatcgcc aatcgcgacc gccagcggat aaggccacgc ctcgcgcaag ccgtaaccca
     3181 gcgtcatcca cagcaccggt aacggtgacg cgacgagaat cgaccagaaa agagtgcgca
     3241 acgtcagcca gaagtgatcc tgagtcactt tgccgacttt cgccgccgaa cgttcaagaa
     3301 aacgggtgaa atagcggcgc gagtaaatac tgcaaccgac cagaatcaac gcgccaaaca
     3361 gcggcaaaat tgtctctttg ctggtcagca tcatcacact ggctttgccc aactgactga
     3421 aggtgtccag cgaaatgaga cgacgcagat cctgggcgat ttccagcggc caggcgatgg
     3481 tcatcgggcg cacgtcagag gcccagaaca gatagcggtg cgttgcttcg ttcacctctt
     3541 tcagcgcatc ctccagttgc ctgttggaga ctttcagctt ggtcagttcc aatagtagcg
     3601 tgtcgccacc ctgcaacaat gagttcagca actcacgctg agtgcgcagt tgtgcttcca
     3661 gaatacggtt ttgctcggcg gtcagcggct gaccgtcggc ctgatggatt tgccgtagca
     3721 gcggctgttt attaagtaaa tcctcataac gtaaccgttg cacacgcaac tgcgccattt
     3781 cggtatcaag ctgttgtggt tttggcattt ctggtagccg tgccacctgc gcccgcagcg
     3841 cttcgccgag caggttggac gatcccagcc attgcgactg ttcacgcagc gtattcaacg
     3901 cctgccggac ctgtaacgtc tggctggcag cctgacgctg ttgcgaggca acgagctcca
     3961 tccgctgcgc ctgttgattc agcgctgccg atagttcgcg gttaattttg aattgcgcga
     4021 cgatatcttt cggcaaatcg gcactgtttt ctgccagtaa ttcggtactt tccagcgccc
     4081 gctccgcctc aagctgacgt tggctattca actgattacg taaggcctgc aaatacgcat
     4141 ccagttgctg gctctctttt tccgccagct ctgagcgtaa gcgcgctaat tcctggcggt
     4201 tattggcaga cagctgcgcc agctccagtt catcaacgag cgccttaaga cgtgcagagt
     4261 cagactgcaa cgcgaaattt tgtgcctgat tgagcggagt attgccggta agcgttccca
     4321 ggcggcgctc gatctcattt aactgacggc gggcgtcggt ttgctgttgc ggcagttgat
     4381 tcagcgaatc ggcaatctcg cgggcgcgct cctgctcttg ctgggcctga cggcttttat
     4441 ccagcaactg gctgctgacc tggagaattt cctgattcag cgcgtcggta gacattcccg
     4501 gcgacacgct gcgcggctcg tcacgaatgt tgtttaattg tgcgcgcaga gtagcggaga
     4561 gtttcggata attatcgata acttgctgat attgtttgat gcgctcaagg gaaccttttc
     4621 gttcctcaag cgcatttaag gcagactgga gcgcctctac gacttccggc tgtgcgggtt
     4681 tcgccgcttt tgcctgctcc agttcctgag tgatttgttt gctatcgggg gccgtcgcgg
     4741 cgtacgcccc ccaactgagg caccaggcca tcagaaaagt gataatcagg cgcacgtcag
     4801 cgtttccttt gatggattag acctggtctt ttttatcgtc aaccaatggg ctggcgtcgt
     4861 gttctgcttc gatctcttca gcaggaagtg gggcgggttc agcgtctggc gtaacaaagg
     4921 tttcggtaga tactgccagc ggctggccaa ttttcgtgac agacaggctt tccagttgct
     4981 caaccagatt cactttaccc ggtgcaaaca ggttgataac ggtggaaccg agtttaaagc
     5041 gacccatttc ctggcctttc agcagtgcca cagaaccgtc gttttccccg gcaggccagg
     5101 tccagcgctt gatgatacct tcgcgcggcg gcgtaatggt gcccgcccag accgtctcaa
     5161 tgctgccaac aatcgtcgct ccgaccagaa tctgcgccat tgggccaaat tcggtatcga
     5221 aaaggcaaat cacgcgttcg ttacgggcaa acagatttgg cacgttctga gcggtgagat
     5281 ggttaacgga aaagagatcg cccggcacgt agatcatctc acgcagaata ccgttgcacg
     5341 gcatgtgtac gcgatggtag tcacgcgggg agaggtaagt ggtcacaaac gtaccgttgc
     5401 ggaacaggtc cgccatcaga tagttgcctg ccagcagggc ttcgaggctg tagttgtggc
     5461 ctttggcttg caggatttta tcttcttcga ttttacccaa ctggctgata acgccatcgg
     5521 caggcatgac cagtacattc ggatcggtat cgattgggcg tacttcgtca cgcagcggac
     5581 ggacaaagaa ttcgttaaag gtgcggtagc tggcggtgtc cggcttttgc gcctctttca
     5641 tgtcgacctt gtagtattta acgaacagat cgataaccag ttttgtcagc catcctgccc
     5701 gcttgcttgc gccccaaccc gccaggcgag taagccatag tttcggcaga atgtactgta
     5761 gcgaaagttt aaatgaattt aacaaggtag cctccaggcc attgttttgt cgttcctgat
     5821 ccggccaaca tgccggatcc tgaaaaaaag gggacgattc taacgacggt tagcttaatt
     5881 gtcagtcatc cgtatcagaa aagtttttac gcgtttttac ctgcgccatg ctttccagaa
     5941 tacggtgata gttttcgaaa cgggtttccg cgattttccc ttcctcaacc gcttcccgga
     6001 tagcgcagcc cggatcggta tcgtgtttgc aatcgcgata tttacacaga cctaaatagt
     6061 catggaattc gacaaagccc tgagtgattt gttccggctc caggtgccag aggccgaact
     6121 cacgcacacc aggggaatca atcacatcac cgccgtgcgg gaagtgatac agccgagcgg
     6181 cggtcgtggt gtgctgaccg agccccgagt tgtcagagac atcgttggtc aggatctctt
     6241 tttgcagccc cagcaacgcg ttcagcaggc tggatttacc gacgccagac tgcccggcaa
     6301 aaatgctgat gcgcccggtc aacgcctctt ccagcggttt cagcccatcc tgagtatggc
     6361 tggaaaccat caatacgcga taaccgatgt tgcggtagat atccatctgc tcgttgacga
     6421 acgccatgcc ttcgtcgtcc agcaggtcta tcttgttgag cacgataatc ggctcaatct
     6481 gcaaggtttc gcaggccacc aggtaacggt cgataatatt gagcgacagc tccggcaaaa
     6541 tggcggagac aatgacaatc tggtcgatgt tggcggctat gggtttcacg ccgtcgtaga
     6601 aatccggacg cgttaacacc gaggtgcgct catgcaccgc ttccacgatc cctttgacat
     6661 ttacgccttc cgccgccggt ttacccggac gccagactac gcggtcgccg gttaccagcg
     6721 aacggatggt acggcgaata ttgcagcggt gaacgtcgcc atcggcggat tccacatcag
     6781 cgtgcatacc aaagcggctg atgacgatac cttcatcagg ctcgccaaac agattgtcgt
     6841 cgtagtcggg cttctcctta gacgttttaa gacgacgctg gtgattggcg ttcacgcggc
     6901 gctgctggcc tttggagagt ttatttttac tcaatcgtac tgactcctgg tcgcccctga
     6961 tgggcaaaac atctatgata cacgcaattg tggatcaata tagtcactgt gaatgggtgg
     7021 aaaatagcat gagtgccaat gaaaacaacc tgatttggat cgatcttgag atgaccggtc
     7081 tggatcccga gcgcgatcgc attattgaga ttgccacgct ggtgaccgat gccaatctga
     7141 atattctggc agaagggccg accattgcag tacatcagtc cgatgaacag ctggcgctga
     7201 tggatgactg gaacgtgcgc acccataccg ccagcgggct ggtagagcgc gtgaaagcga
     7261 gcacgatggg cgatcgggaa gctgaactgg caacgctcga attcttaaaa cagtgggtgc
     7321 ctgcgggaaa atcgccgatt tgcggtaaca gcatcggaca ggaccgtcgt ttcctgttta
     7381 aatacatgcc ggagctggaa gtctacttcc actaccgtta tctcgatgtc agcaccctga
     7441 aagagctggc gcgccgctgg aagccggaaa ttctggatgg ttttaccaag caggggacgc
     7501 atcaggcgat ggatgatatc cgtgaatcgg tggcggagct ggcttactac cgcgagcatt
     7561 ttatcaagct gtaaaatttt tcctggtcac gtaagcgccg gaatccactt gcggcggcgt
     7621 gattttgacg ctaaatcgaa cgtcttgctg ataatttcag cacttgagat aaaaacgcaa
     7681 aaaaaacttt tttggggggt tgcagaggga aagatttctc gtataatgcg cctcccgtaa
     7741 cgacgcagaa atgcgaaaat tacgaaagca aaattaagta gtacgcggga atagctcagt
     7801 tggtagagca cgaccttg
//
LOCUS       DAJTUI010000149         7743 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTUI010000149 DAJTUI010000000
VERSION     DAJTUI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.149"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..1015
                     /gene="ydfJ"
                     /locus_tag="OO464_003723"
     CDS             <1..1015
                     /gene="ydfJ"
                     /locus_tag="OO464_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR6228717.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1104..2564
                     /locus_tag="OO464_003724"
     CDS             1104..2564
                     /locus_tag="OO464_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6228718.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="OO464_003725"
     CDS             complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="OO464_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6228719.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2981..3667)
                     /gene="ydfH"
                     /locus_tag="OO464_003726"
     CDS             complement(2981..3667)
                     /gene="ydfH"
                     /locus_tag="OO464_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR6228720.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="OO464_003727"
     CDS             complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="OO464_003727"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6228721.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4639..6684
                     /gene="dcp"
                     /locus_tag="OO464_003728"
     CDS             4639..6684
                     /gene="dcp"
                     /locus_tag="OO464_003728"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6228722.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6729..7247)
                     /locus_tag="OO464_003729"
     CDS             complement(6729..7247)
                     /locus_tag="OO464_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR6228723.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7523..>7743
                     /gene="ydeI"
                     /locus_tag="OO464_003730"
     CDS             7523..>7743
                     /gene="ydeI"
                     /locus_tag="OO464_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR6228724.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISH"
BASE COUNT         1940 a         1812 c         1955 g         2036 t
ORIGIN      
        1 catcttgctg gtgacgctgc gtattattca gggattgggt gcaggtgcgg aaatttccgg
       61 tgccggtacg atgctggcgg aatatgcgcc aaaaggtaag cgcggaatta tctcctcatt
      121 tgtggctatg ggaactaact gcggcacctt aagcgcaacg gcaatctggg cctttatgtt
      181 cttcattctc agtaaagagg aactgctggc gtggggatgg cgtataccgt tcctggcgag
      241 cgttgtcgtg atggtctttg ctatctggtt gcgtatgaat ctgaaagaaa gcccggtctt
      301 tgagaaggtt aacgacagca accaaccgac agcaaaacct gcacctgctg gtagcatgtt
      361 ccagagcaaa tccttctggc tggcaacagg gctgcgtttt ggtcaggcgg gtaactccgg
      421 tttaattcag actttccttg caggctattt agtacagacg ttattgttta acaaagcaat
      481 tccaacagat gcattgatga tcagttcgat tctcggcttt atgaccattc cgttccttgg
      541 ttggttatcc gataaaattg gtcgccggat cccgtatatt attatgaata cctctgcgat
      601 tgtgctggcg tggccaatgc tttctatcat cgtagataaa agctatgccc cgagcaccat
      661 tatggttgca ctgattgtga ttcataactg tgcggtgctg ggattatttg ctctggaaaa
      721 cattaccatg gcagaaatgt tcggctgtaa aaaccgcttc acccggatgg ctatttcaaa
      781 agaaattggt ggtcttatcg cttccggttt tggtcctatc ctggcgggta ttttctgcac
      841 catgacggaa tcctggtatc cgatcgccat tatgatcatg gcatattcag tgattggttt
      901 aatctctgcg ctgaaaatgc cagaggtgaa agaccgtgat ttaagtgcgc tggaagacgc
      961 tgcggaagat caaccgcgtg ttgtaagagc tgcgcaacct tccagaagtc tgtaaaccct
     1021 caatcccttc tcttaccgga gaggggattt ttattcatat aacaaaacat atagcttgcc
     1081 atatttaaat ttaaggaatt gttatgggaa ataatttgtt atcagcaaaa gcgacactcc
     1141 ctgtttatga tcgtaataac ctggccccaa gaattgttca tttaggcttt ggtgcatttc
     1201 accgtgcgca tcagggcgtg tatgccgata ttcttgctac agaacatttc agtgactggg
     1261 gatattatga ggtcaactta atcggcggcg aacagcaaat tgccgattta caacagcaag
     1321 ataatcttta taccgttgcg gaaatgtcgg ccgatgcgtg gacggctcgc gtcgttggcg
     1381 tcgttaaaaa agccttgcac gtacagatgg atggcttaga aaccgtgttg gctgcgatgt
     1441 gtgaaccgca aatcgcgatt gtctctctga caatcaccga aaaagggtat ttccactctc
     1501 cggcgaccgg acagttaatg ctcgagcacc cgatggtcgc tgccgacgtg caaaatcccc
     1561 accagccgaa aacagcaaca ggggtgattg ttgaggcgct ggctcgccgt aaagcggcag
     1621 gacttcccgc atttaccgtc atgtcatgtg acaacatgcc agaaaacggt catgttatgc
     1681 gtgacgttgt cacttcctac gcgcaagccg ttgatgtaaa actggcacaa tggatcgaag
     1741 ataacgtgac tttcccatca acaatggtgg accgtattgt gcccgcagtg acagaggata
     1801 cgctggcgaa aatcgaacaa cttaccggtg tgcgcgatcc tgctggcgtt gcctgtgaac
     1861 ctttccgcca gtgggttatc gaagataact ttgtagccgg acgtccggaa tgggaaaaag
     1921 cgggagccga actggttagt gatgtgctgc cttatgaaga gatgaagttg cgcatgctca
     1981 acggcagtca ttcattcttg gcatatctgg gttatcttgc tggatatcag cacattaatg
     2041 actgtatgga agatgaacat tatcgtcatg cggcgtatgg catgatgttg caggaacaag
     2101 cgccgacgct gaaagtgcag ggcgttgatt tgcaagatta cgctaaccga ttaattgcac
     2161 gctatagcaa cccggcgtta cgtcatcgaa cctggcagat tgcgatggat ggcagccaga
     2221 aattgccaca gcggatgttg gattctgttc gctggcatct ggcgcatgac agcaagttcg
     2281 atctgctggc gctgggcgtc gcgggttgga tgcgttatgt cggtggtgtt gatgaacagg
     2341 gaaatccgat agaaatcagt gacccactgt tacctgttat tcagaaggct gtacaaagta
     2401 gtgccgaagg gaaagcgcgc gtccagtcat tgctggcgat taaggcaatc tttggtgatg
     2461 atttgccaga caatagcttg tttactgcaa aagtgacgga agcgtacttg tctttattag
     2521 cgcatggcgc gaaagcgacc gtggcgaaat attccgtgaa gtaaaaaaag caatgccgcc
     2581 atcggcggca ttcacacact tagttcatgc cgagacgaat caggtccaga ggtgccagtt
     2641 tctcttcaca accttcaaca actacggttt ttccgcggcg gatttgctca gcggtcagac
     2701 cttcagtatc aatcgacagg atcttgccag tgtgaccggt gccgctaacc ataacacggc
     2761 tgccagtggt gattgcgtta cggttacgat cgtaggtcgt cataggtgtt ttctcctttc
     2821 tgatttacag tcgaagccca cgtaacgggc gcaatataaa tactcctgtc gagtgaaata
     2881 ttttttgttt ttgatcaagc tcacatcttt tttgatgcat gcatgagccg taaggcgggg
     2941 ggaaaagcct ctcatcccgg atgggatgag agtaaatgaa ttactcttcg ctaaaccagt
     3001 cgctgttttc ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa tgttgcgtca
     3061 ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc agagaaaata tcaagatgct
     3121 ggcgtaacag catttctggt ggagaaacat ggtcgaaact catatagcgc acgcgatcaa
     3181 cggtcgcttt caggttctca atggtatccc acgccagttg acagtcggca atctgcgtca
     3241 ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg cgctcaatgg
     3301 caatgcgttg ctggtgaaga ttttgttcca gttggtagca ctggctttcg gtaatcatgc
     3361 tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg gataaaactg ccgttgcgca
     3421 cctgggccat ggaaattttg ttgacgtagc tgccacgctg cggacgaatt tgaatcaggc
     3481 cgttttccgc cagtttaata aaggcttcac gaaccggctg gcgtgacaca ttgaaacgaa
     3541 cagaaacttc tttttccgac aacggtgtgc ctggtgcaat caggcaatgg acaatgtcgc
     3601 gacgaagaat acgataaatc tgctgattga caggctgtgt gggattaagt tgcgtttcga
     3661 cggtcattcg ttcttgcttg tgagtgagtt aactgcatga gtctaccact tttttgtgac
     3721 aagcaataac ccggcagtta cgccgggtat aaaaattact gacggtggac attcagtccg
     3781 gcatagcttt gggtaaccgg catcatttcc agggtattga tattgacgtg agcaggcagc
     3841 gttgacaccc accagacggc ttcgctgaca tcttctggcg tcaatgcaac ggtattttga
     3901 taggtttttt ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa ctcggtgcca
     3961 cccaccagac ccggttcgat gtcggtgacg cgcaccgccg taccatgcag atccgtacgc
     4021 agattcaggc taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt accaccggca
     4081 tacggccagc tgcctgccgt tgagccaatg ttaataatat gaccatgatt acgttcaacc
     4141 atacccggta agacggcgcg cgtcatatat actaggcctt tgttgttggt atcaatcatc
     4201 gtttcccagt cttcaacgct ggctttatgc gcaggctcca tgcccaacgc caggccggca
     4261 ttatttacca ggatatcaat attgctccac tcggcaggga gcgatgccag catctcttca
     4321 atagcggcgc ggttgcgaac gtccagttgg gcgatataca gattatctcc cagttcgtct
     4381 gttaactcct gcaaccgctc ctggcgacgg ccagtggcga taactttatg cccttgttga
     4441 ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg ctccagttac taaaacgatc
     4501 atctcactgt tcctcaacgc ttttgtgtgt caataacata gcacgtgatg cttaagagcg
     4561 ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa gcctacgcta tggaacacct
     4621 cgcgttcagg agaagaaaat gacaacaatg aatcctttcc ttgtgcaaag cacactgccg
     4681 tatctggctc ctcattttga tcaaattgcc aatcatcact atcgcccggc attcgatgag
     4741 ggaatgcaac aaaagcgggc agaaattgct gccatcgcgc ttaacccgca aacgcctgat
     4801 ttcaacaata ctattctggc actggaacaa agcggagaat taattacccg cgttaccagc
     4861 gtcttttttg cgatgactgc ggcgcatacc aatgatgaat tacagcgtct tgatgaacag
     4921 ttttccgctg aactggcgga actggctaat gatatctatc tgaacggtga attattcgcg
     4981 cgggtagatg ctgtctggca gcgccgtgaa tccctggggc ttgatagtga atccatccgc
     5041 ctggtggagg ttattcatca acgttttgtc cttgccggag ccaaacttgc acaagctgat
     5101 aaagcaaaat taaaagtact gaatacagaa gctgcgaccc tgaccagcca gtttaaccag
     5161 cggttacttg cagcaaataa atccggcggt ctggttgtga acgatatcgc gcagctggca
     5221 ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg ctcgcgagaa aggtctggat
     5281 aacaaatggc tgattccgct gctgaatacc acccaacaac cggcgcttgc cgaaatgcgc
     5341 gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga cgcgagcgga aaaaaatgat
     5401 gccaatgata cccgcgctat cattcaacgt ctggtagaga ttcgcgcaca gcaggcgaaa
     5461 ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg atcagatggc aaaaacgcca
     5521 gaagcagcac tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg tgcgagcgat
     5581 gaattagcct ccatacaggc ggttatcgat aagcagcagg gcgggtttag cgcgcagccg
     5641 tgggactggg cattttatgc ggaacaggta cggcgggaga aatttgatct tgatgaggcg
     5701 cagctcaagc catattttga attaaacacg gtgttaaatg aaggtgtatt ctggaccgcg
     5761 aatcagctct tcggtattaa gtttgtcgaa cgttttgata ttccggtcta ccatcctgac
     5821 gttcgtgtgt gggaaatttt tgatcataat ggcgtgggac tggcgttatt ttacggtgat
     5881 ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg gcaattttgt tgagcaatca
     5941 acgcttaatg aaacgcatcc ggtaatttat aacgtctgca attatcagaa acccgctgcc
     6001 ggtgagcctg cgttgttact ctgggatgat gtaataacct tattccatga atttggtcat
     6061 acgctgcacg gcctttttgc ccgccagcgt tatgccacgc tttccggcac caacacgccg
     6121 cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca tccgcaggta
     6181 ttcgctcgct acgcccggca ttatcagagc ggggcagcaa tgcctgacga actgcaacag
     6241 aaaatgcgta atgccagcct gttcaacaaa gggtatgaga tgagcgaact gcttagcgcc
     6301 gcacttctcg atatgcgctg gcattgcctg gaagaaaacg aagcaatgca ggatgtcgat
     6361 gattttgaat tgcgggcgct ggtggcggaa aatatggatc ttcctgctat accgccacgc
     6421 tatcgcagca gttatttcgc ccatattttt ggtggcggat atgctgcagg ttattacgct
     6481 tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt ggtttgttga gcagggcgga
     6541 ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc tttccagagg taacagcgaa
     6601 gatctggaac gcctgcatcg acaatggcgt ggtaaggctc ctcagattat gccgatgctg
     6661 caacatcgtg gcttgaacat ataaacacca ccaatggctg gcctggagat atcgccaggc
     6721 cagcctggtt atagcagtaa ttgcagcagc tgggcgaggg caaacctcac cgctaaagct
     6781 aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag tgtagttctg gcctttaata
     6841 tgccacgcga accagacggt acccgctggc gtaccatctt caccgccctc cggtccgccg
     6901 tagccggtaa tggcaatact gacatcagca tccgcacgct ctatggcacc tgttgccatt
     6961 tctgctgcca ctttctcact caccgcagaa tatcgttcaa gagactgctg gcttacgctg
     7021 aggattttca tctttgcctg atcggtgaaa gtaacaaagc ctgcaccgta aaatttgggt
     7081 gtatcttcag ctgcacacag ggcgctagcc aacttaccgc cggtgcatga ttcggcggta
     7141 gttaagcgta attgatccgc gataagtctt tgactcaacg cggatgtcag gttttcaata
     7201 gtgtcggtat ctgcgagttg tacaatttta tctttattaa tgttcactgt tttttgtcca
     7261 cgctattata tgtaaaggtg atttgctttt tctgtatgct cgtccaatat aataattttc
     7321 aaacatataa atccgattat tctgaaaaca tgtttaagac tcttcaatct tcttaacttt
     7381 agtgagtcaa attctctgaa tatttgacag ctagtgggct gattttaaat gccttataac
     7441 aattggcagt attcttattt ggcaattata aaaatagcaa ccagacttaa taagtcaaac
     7501 caaacgcaat taaggagtta gtatgaaatt tcagtccatt gtattagcaa gttttcttgt
     7561 catgccttat gcgttggcag atgaccaggg cggtttaaaa caggatgcag cgccaccgcc
     7621 gcctcatgca atagaagatg gctatcgcgg aaccgatgat gcaaaaaaaa tgaccgttga
     7681 tttcgcaaaa aacatgcacg atggcgcttc ggtttcacta cgcggtaatt tgatttctca
     7741 caa
//
LOCUS       DAJTUI010000150         7721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTUI010000150 DAJTUI010000000
VERSION     DAJTUI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.150"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..832)
                     /locus_tag="OO464_003731"
     CDS             complement(<1..832)
                     /locus_tag="OO464_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCR6228725.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     E"
     gene            1014..2438
                     /gene="sbcB"
                     /locus_tag="OO464_003732"
     CDS             1014..2438
                     /gene="sbcB"
                     /locus_tag="OO464_003732"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6228726.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2481..2708)
                     /locus_tag="OO464_003733"
     CDS             complement(2481..2708)
                     /locus_tag="OO464_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR6228727.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2722..3780)
                     /gene="yeeE"
                     /locus_tag="OO464_003734"
     CDS             complement(2722..3780)
                     /gene="yeeE"
                     /locus_tag="OO464_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR6228728.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="OO464_003735"
     CDS             complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="OO464_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6228729.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="OO464_003736"
     CDS             complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="OO464_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6228730.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5530..6513)
                     /locus_tag="OO464_003737"
     CDS             complement(5530..6513)
                     /locus_tag="OO464_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6228731.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6559..7383)
                     /locus_tag="OO464_003738"
     CDS             complement(6559..7383)
                     /locus_tag="OO464_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6228732.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="OO464_003739"
     CDS             complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="OO464_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR6228733.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2067 a         2012 c         1812 g         1830 t
ORIGIN      
        1 gttcgaagat tggacggcgg ataacattac ccatcttgct atgctctttc ggtacggtcc
       61 atacgttgtc cagcaggtca aattctgtct ttgttgccag cctaagctct gagagcctcg
      121 ccccccacag cataagcatc tgatgaagga gcttatttga cgtagacgca cggcttcttt
      181 caatagcaag ccaaatctta gccagttcgt gatacgacag tacccgatcc cctacctcag
      241 cgcgggagcc gaagtccctt ggctggatgc tcataattgc gcaactatct atcaactgac
      301 gccgcatgca ccaactaatt gctgatctta gttgacttag cacctgcctt gctcggcgtg
      361 gattatcttt ttcttcttcg gtaagcaggt caacccattg cttaaccgtg atagaagatg
      421 ccggacgatt agggaaggcg tcatgcatgc gcttcataac cgttgatcga taaagtgcct
      481 gggtcttttc tctgagagtt gtagagacgt agttgtcgaa ccagtagtcg agacactggg
      541 cgaccgtcat ggagttctcc accttctctt caaaataggt gcgtggatcc gttcctgaga
      601 aatagagctt tcgcaagtca gcagtgatct gtctggcatc cttcaaagac agggatgggt
      661 atcgaccaag cccaagtcga ttaggcttgc catgccagcg atagcggtac tggaactgga
      721 tgaccccctt cggtgaaatt cgtacgctga ggccatcggc atcagccact tcttgtgggc
      781 ccgaatatgg tttaccataa atagtacgca gttttgtgtc gcttatagcc ataaataata
      841 tttggtacgc tcagaaataa tagtttggca cacatctggt acacaatatc acatggacaa
      901 cagcgcacag taaacaacta tatttgacgt tcgtagacat actcaggtga aaaaagggtt
      961 gcttttcgag ttataacaga cagttaatta actacagtgg acaatgctag acaatgacag
     1021 acacagataa acaacctacc ttcctctttc acgattacga aacctttggc acgcaccccg
     1081 cgttagatcg ccctgcacag ttcgcagcca ttcgcaccga tgacgaattc aatgtcatcg
     1141 gcgaacccga agtcttttac tgcaagcccg cggatgacta tttaccccgg cctggagcag
     1201 tattaattac cggtattacc ccgcaggagg cacgggcgaa aggagaaaac gaagccgcgt
     1261 ttgccgcccg tattcactcg ctttttaccg taccgaagac ctgtattctg ggctacaaca
     1321 atgtgcgttt cgacgacgaa gtcacacgca acgtttttta tcgtaatttc tacgatcctt
     1381 acgcctggag ctggcagcat gataactcgc gctgggattt actggatgtt atgcgtgcct
     1441 gttatgccct gcgcccggaa ggaataaact ggcctgaaaa tgatgacggt ctaccgagct
     1501 ttcgccttga gcatttaacc aaagcgaatg gtattgaaca tagcaacgcc cacgatgcga
     1561 tggctgatgt gtacgccact attgcgatgg cgaaactggt aaaaacgcgt cagccacgcc
     1621 tgtttgatta tctctttacc catcgtaata aacacaaact gatggcgttg attgatgttc
     1681 cgcagatgaa acccctggct cacgtttccg gaatgtttgg ggcatggcgt ggcaatacca
     1741 gctgggtggc accgctggcg tggcatcctg aaaatcgcaa tgccgtaatt atggcggatt
     1801 tggcaggaga catttcgcca ttactggagc tggatatcga cacattgcgc gagcgtttat
     1861 ataccgcaaa agccgatctt ggcgataacg ccgccgttcc ggttaagctg gtgcatatca
     1921 ataaatgtcc ggtactggcc caggcgaata cgctacgccc ggaagatgcc gaccgactgg
     1981 gaattaatcg tcagcattgc ctcgataacc taaaaattct gcgtgaaaat ccgcaagtgc
     2041 gtgaaaaagt ggtagcgata ttcgcggaag ccgaaccgtt tacaccttca gataatgtgg
     2101 atgcacagct ttataatggc tttttcagtg acgccgatcg tgcagcaatg aaaattgtgc
     2161 tggaaaccga gccgcgtaat ttaccggcac tggatatcac ttttgttgat aaacgaattg
     2221 aaaagttgtt gttcaattat cgggcacgca acttcccggg gacgctggat tatgccgagc
     2281 agcaacgctg gctggagcac cgccaccagg tcttcacgcc agagtttttg cagggttatg
     2341 ctgatgaatt gcagatgctg gtacaacaat atgccgatga caaagagaaa gtggcgctgt
     2401 taaaagcact ttggcagtac gcggaagaga tcgtctaatt tgaagccgcc gccgctggta
     2461 cggcggagga caatgttgag tcaggctttt tgaacggtga tgctccacgc tgcatcgcca
     2521 atctgctgat aatcggtgat ggcatgtcct tcttctgccg cccactgtgg gattgcttca
     2581 gttgcctggg tgcaatcaaa ctcaattacc aactcatcgc cgctggccat ttctgccagt
     2641 gctgcttttg cttctatgag tggaaacgga cagacctgcg tcaccacatc cagcttttta
     2701 atcgccatct ttacacctta cttaatttgc cgcagcagtt gccagtcgcg ccttacgctg
     2761 cggtcgaaca tacacaagcc aggacgcagt ccagactccg agaatcataa atacaaggcc
     2821 aatccagccc tgccaggtca tcattgcagt cataaccagt ccattaccga tagagcaacc
     2881 acctgcaata ctggcaccga agcccatcag tacaccacct aacccgctac gtaatgttgt
     2941 ttgtgcatca gctgcgcgaa cgcggaactc acggctcgct ttggctgcaa taaatgcctc
     3001 cacgaagatc cctaacacca ggaaaacgcc ccagttaatg tatttcacgt cacccgcgac
     3061 cagaaattga agaatattgg ccgttgggga agtgactcct aacccaaaca tgcgtccggt
     3121 tgcttcactc aggggccagg ctaaaagcgc aatcaaaccg atgagtacag cagtgacaaa
     3181 gggatgccag cgtttttcaa acagaatatg agcgatcccg gttcggcgcg gcggtaaggt
     3241 cgcgactttg agttttggct tcttcaactc tttcatcacc acccagagcg ttatcaccag
     3301 caaaacggta accaacggcc acacagacaa attaaaagtc tcagcaatag agttatgttc
     3361 agtagtgtag tgctgcaagg tttgatttaa accactggcg tgtggagaac gcatcaccgc
     3421 actcatcacc atataagtga aaagcgcgat ccaactgccg atcaatccct cacccgcgcg
     3481 ataccaggtc ccggtggcac atccacccgc cagaacaatt cccagcccga aaagataccc
     3541 acctataaca gtaccaagcc acgggaacgc cccggcttcg taagtcagta ggcccgcctg
     3601 aattaacgca aaaaccccca cgctttgtgc cgaaattgca atcagcaagg cgtaaaacat
     3661 gcgattattt ttcgcgatat acatatcgcg aaaaccacca gtcaggcaaa aacgcccacg
     3721 ctgcatgaca aatcccagca gagcaccaca aattagcccg cttaatatca ttgaaaacat
     3781 aaattaatta accagatgaa tgttaatggg gaaatttttc atgactggtg gaatgcagac
     3841 caataaccaa attctctaaa ttagaacaaa tggttattaa tgaggatttc gataaaaaag
     3901 aataaaaaaa ccggagccaa atgttcattc gactccggtt tattattaga aaagatgatt
     3961 atgctacgtc ttcgtactgc ggaaccggat tacggaagct cttggtgacg caagccaggt
     4021 aaatcagacc gatagcagcc cagatcagac ccagaaccat tgagctttct tccaggttaa
     4081 cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac tggaagtgat
     4141 ctttcagcgt cttgttacgc ttctcacgga tccagaactg cgagatgacc gacaggttaa
     4201 caaaggtgaa cgccaccagc gcaccaaagt taatcagcgc cgttgccatt accaggtcga
     4261 agttgattgc cagcagggca atcgcgccaa ccaggatgat gttcatcgcc ggagtacgcc
     4321 atttcgggtg tacataaccg aagaagcttt tcgggaacac gccgtcacga cccattacgt
     4381 acatcagacg cgctacgcct gcgtgcgccg ccataccgga cgccagtacg gtaatggtgg
     4441 agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg atttcaggct
     4501 gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc aggaagtaag
     4561 ttgcaaagat gaagatcatg ccgccaatca gcgcggtcag gaaaatcgca cgcgggatca
     4621 cgcgctctgc atctttggtt tcttccgaca gattgctgat gccgtcaaag ccggtaaagg
     4681 agaagcacag gattgtcgcc ccggtaatca tcgggataac atgcgcatcg ccagaccaga
     4741 acggacgcgt gctcgccagc gtaccagcgc cttcaccttc aaatacgcca taaacaacca
     4801 tgcccagaat caccgcgatc agcactacct gcaacacgac gattacagtg ttgacgttcg
     4861 ctacggattt cagactacgc aggttaaagg cggtcataaa ggccaccagc gccaccacaa
     4921 acatccacga tggaatggaa ggcaccagag cttcaaaata gattttcgcc agcagaatgt
     4981 tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata aagccgacag
     5041 tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc agcagaagga tagcggcgaa
     5101 ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac gcataggctg
     5161 tcggcacatg accatccgtg aggcctgaaa cgataccaaa tgtatcaaac agcgtcatcg
     5221 gctgcatata ggctagaccc atcattacaa ccggaactaa cgtaagcgtt ttacgcaatt
     5281 ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt tattctcctt tacggtgata
     5341 accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt cctcagcgac aacaactgtc
     5401 gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa tgattggttt
     5461 gaagcaaaaa ataaccgacg ctaattaaac gtcggttttt aatcattttt tgacagcggc
     5521 gcattgtgcc taaatggggg ggaaatgaca agagaatgag aggcttgtca taataatttt
     5581 tctttgaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga tcgcactatt
     5641 tgctaaaatt tcatctcgcc accagccgct ggcaagccct gcggtctgtt cattccagcc
     5701 aatccagaca ggttcaaaga gagtttgggc gaccactttc ttctcgatta acgcgccact
     5761 atcgagaaaa cgttgcgcca gataccgtgg taaatagccg cagccaaggc cgctaatttg
     5821 cagctccagt ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt cgagaagttg
     5881 tgaggccgta gaagcgcctg cctgcgcagt atcccccacc acaatcgccc gatatcgttt
     5941 aatgatacga cggtttaacg gttcttcttc ctgggctaac ggatgatgag gcgcgaccgc
     6001 aaagacttgt tcaagatcgc ccaaccgcga gaaaccaaac tcactggaag atggtggctc
     6061 atgcatcgcg ccaacgataa tatccgctcg cccctgagtc agggcatccc aggaaccacc
     6121 gagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa aagcttcaat
     6181 gagaggcgca agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca gctcgttttc
     6241 ccagccttca tgcagtttga tcgcctgctt ttccagttct cgcacggtat gcagaacttc
     6301 ccgccctttc tctaataaca tttttccggt gcgggtgaat ttagcgcggt gtccgctacg
     6361 atcaagcaat tgaatattta ggtcgctttc cagcttgtga acggtgtaac tcagagcgga
     6421 cggcgtcttg tagagtttgg ctgacgccgc cgcaaaactg ccctcttttt ctatggcatc
     6481 gaggatcatc aacacatcca gcagtggttt catacacgcc cccttgcagt gcatgcggcg
     6541 tcctgctggc gtgatcgttt actccaacgg cattaccagc ggatcgggat actggtattc
     6601 aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc cgctgtccag
     6661 actatttctg aactgcggag gttgcagccc cagtaaacgg gccatctgcg gatagaaaac
     6721 attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt taggtgcctg
     6781 taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa caccatgttc
     6841 accatcaggg gcggtttttc cggcaaagaa gcgtcccgga tgacgtcccg gtccgaccag
     6901 gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc agtcttcgag
     6961 ttcttctaat actcgtccac tgttggttac cggattacgc ggggtagttt ctttaaccgt
     7021 gccttgcgcg tcgccataga cagatgttga gctggtaaaa ataattcggg gaatacgatg
     7081 ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat cgccggggcc
     7141 gctacgacgt gccggaagcg taatgaccag cgcatcggca tccataaggg catccagatc
     7201 gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa tgccactcat
     7261 tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc agcctcgcgc
     7321 tgaaagtgac atcgccagcg gcatacctaa ccaccctaac ccgacaattg cgaccttttt
     7381 catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta tcaaaactga
     7441 caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca atgagcagtg
     7501 aatcatcagt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg gaccagaaac
     7561 ctgacaaatt atgtttcagg ggttctggtt tccccttacc ttcaaatggt gttctgcggg
     7621 tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc tgccagtaca
     7681 gataatcatc ccatgattcc tcagaccaga ttagtttcac t
//
LOCUS       DAJTUI010000151         7536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTUI010000151 DAJTUI010000000
VERSION     DAJTUI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.151"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..1159
                     /gene="rbsA"
                     /locus_tag="OO464_003740"
                     /pseudo
     CDS             1..1159
                     /gene="rbsA"
                     /locus_tag="OO464_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1164..2129
                     /gene="rbsC"
                     /locus_tag="OO464_003741"
     CDS             1164..2129
                     /gene="rbsC"
                     /locus_tag="OO464_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6228734.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2154..3044
                     /gene="rbsB"
                     /locus_tag="OO464_003742"
                     /pseudo
     CDS             2154..3044
                     /gene="rbsB"
                     /locus_tag="OO464_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3170..4099
                     /gene="rbsK"
                     /locus_tag="OO464_003743"
     CDS             3170..4099
                     /gene="rbsK"
                     /locus_tag="OO464_003743"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6228735.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4103..5095
                     /gene="rbsR"
                     /locus_tag="OO464_003744"
     CDS             4103..5095
                     /gene="rbsR"
                     /locus_tag="OO464_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6228736.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5061..6488)
                     /gene="mdtD"
                     /locus_tag="OO464_003745"
     CDS             complement(5061..6488)
                     /gene="mdtD"
                     /locus_tag="OO464_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6228737.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6511..7203)
                     /locus_tag="OO464_003746"
     CDS             complement(6511..7203)
                     /locus_tag="OO464_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6228738.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1917 a         1826 c         1986 g         1807 t
ORIGIN      
        1 aaaccatgta tgtcgaagcg gataaattgc tggctaaact taacctgcgc tttaaaagcg
       61 acaagttagt gggcgatctt tccatcggtg accagcaaat ggttgaaatc gccaaagtgc
      121 tgagctttga gtcgaaagtc atcattatgg atgaaccgac cgatgcgctg accgataccg
      181 aaaccgaatc cctgttccgc gtcatccgcg agctgaaatc gcaaggccac tgtattgtct
      241 atatctccca ccgcatgaaa gaaatcttcg agatttgcga tgacgttacc gtttttcgtg
      301 atgggcaatt tattgctgag cgcgaagtgg catcactgac cgaagattcg ctgattgaga
      361 tgatggtggg ccgcaagctg gaagatcaat atccacacct ggacaaagcg ccaggagata
      421 tccgcctgaa agtcgataat ctctgcggac ctggcgttaa cgatgtctct tttactttac
      481 gcaaaggcga aattcttggc gtctctggtt tgatgggcgc aggtcgtacc gaactgatga
      541 aagtgctcta cggcgcactg ccgcgcacca gcggttacgt caccctggat gggcatgaag
      601 tcgttacccg ttcaccgcag gatggcctgg caaacggcat tgtgtatatc tccgaagacc
      661 gtaaacgtga cggtttagtg ctgggcatgt cagtaaaaga gaacatgtcg ctgacagcgc
      721 tgcgctactt cagccgcgct ggcggcagtt tgaagcatgc cgatgaacag caggctgtga
      781 gtgatttcat tcgtctgttt aatgtgaaaa cgccgtcgat ggaacaggca attggtctgc
      841 tttccggtgg caatcagcaa aaagtggcga ttgcccgcgg tctgatgaca cccccaaagt
      901 attgatcctt gatgagccta cccgtggcgt agatgtcggc gcgaaaaaag agatctatca
      961 actgattaac cagttcaaag ccgatggctt gagtatcatt ctggtgtcat cggagatgcc
     1021 agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc tcagcgggga
     1081 atttactcgt gagcaggcca cccaggaagt gttaatggct gtcgctgtgg gcaagcttaa
     1141 tcgcgtgaat caggagtaaa aaaatgataa cccagactgt ctctggtcgc cgttatttca
     1201 cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg atcgcgattg
     1261 tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt ctccagcaaa
     1321 cttcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg tcgggcatag
     1381 acttgtcggt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct atcgtcggca
     1441 ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcac gttaggtgcc gcaattggtg
     1501 cggtaaccgg ggtgattgta gcgaaaggtc gcgtccaggc gtttatcgct acgctggtta
     1561 tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca gtgaataccg
     1621 gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg ctgggcgtac
     1681 cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg ctgcatcaca
     1741 cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg cgtctttctg
     1801 gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg ctggcatcgc
     1861 tggccgggat cattgaagtg gcgcgtctct cctctgcaca acccacggcg gggactggct
     1921 atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg ggcggaaaag
     1981 gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat aatggattga
     2041 atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg attttgctgg
     2101 cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg aatatgaaca
     2161 tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc agtgcgaatg
     2221 cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg ttctttgtat
     2281 cgctgaaaga tggcgcgcag aaagaggcgg ataaacttgg ctataacctg gtggtgctgg
     2341 actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc gttcgcggca
     2401 ccaaaattct gctgattaac ccgaccgact ccgacgcagt gggtaatgct gtgaagatgg
     2461 ctaactaggc gaacattccg gttatcactc ttgaccgcca ggcaacgaaa ggtgaagtgg
     2521 tgagccacat tgcatctgat aacgtactgg gcggcaaaat cgccggtgat tacatcgcga
     2581 agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgccggt acatccgcag
     2641 cccgtgaacg tggcgaaggc ttccagctgg ccgttgctgc tcacaagttt aatgttcttg
     2701 ccagccagcc agcagatttt gatcgcacca aaggtttgaa cgtaatgcag aacctgttga
     2761 ccgctcatcc ggatgttcag gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc
     2821 tgcgcgcact gcaaactgcc ggtaaatcgg atgtgatggt cgtcggattt gacggtacac
     2881 cggatggcga aaaagcggtg aatgatggca aactagcagc gactatcgct cagctacccg
     2941 atcagattgg cgcgaaaggc gttgaaaccg cagataaagt gctgaaaggc gagaaagttc
     3001 aggctaagta tccggttgat ctgaaactgg ttgttaagca gtagttttaa tcaggttgta
     3061 tgacctgatg gtgacataaa tacgtcatcg acagatgaac gtgtaatata aagaaaagca
     3121 gggcacgcgc caccctaaca cggtggcgca ttttatggac atcccgaata tgcaaaacgc
     3181 aggcagcctc gttgttcttg gcagcattaa tgctgaccac attcttaatc ttcaatcttt
     3241 tcctactcca ggcgaaaccg taaccggtaa ccactatcag gttgcatttg gcggcaaagg
     3301 cgcgaatcag gctgtggctg ctgggcgtag cggtgcgaat atcgcgttta ttgcctgtac
     3361 tggcgatgac agcattggcg agagcgttcg ccagcagctc gccactgata acatcgatat
     3421 tactccggtc agcgtgatca aaggcgaatc aacaggtgtg gcgctgattt ttgttaatgg
     3481 cgaaggtgag aatgtcatcg gtattcatgc cggcgctaat gctgctcttt ccccatcgct
     3541 ggtggaagcg caacgtgagc gtattgccaa cgcgtcagcg ttattaatgc agctggaatc
     3601 accactcgaa agtgtgatgg cagcggcgaa aatcgcccat caaaataaga ctatcgttgc
     3661 gcttaacccg gctccggctc gcgaacttcc tgacgaactg ctggcgctgg tggacattat
     3721 tacgccaaac gaaacggaag cagaaaagct caccggtatt cgtgttgaaa atgatgaaga
     3781 tgcagcgaag gcggcgcagg tactgcatga aaagggtatc cgtactgtac tgattacttt
     3841 aggaagtcgt ggtgtatggg ctagcgtgaa tggtgaaggt cagcgcgttc ctggattccg
     3901 ggtgcaggct gtcgatacca ttgctgccgg agataccttt aacggcgcgt taatcacggc
     3961 gttgctggaa gaaaaaccat tgccagaggc gattcggttt gcccatgctg ccgctgcgat
     4021 tgccgtaaca cgtaaaggcg cacaaccttc cgtaccgtgg cgtgaagaga tcgacgcatt
     4081 tttagacagg cagaggtgac gcttggctac aatgaaagat gttgcccgtt tggcgggcgt
     4141 ttctacctca acagtttctc acgttatcaa taaagatcgc ttcgtcagtg aagcgattac
     4201 cgccaaagtt gaagcggcga ttaaagaact caattacgcg ccatcagctc tggcgcgtag
     4261 cctcaaactc aatcaaacac ataccattgg catgttgatc actgccagta ccaatccttt
     4321 ctattcagaa ctggtgcgtg gcgttgaacg cagctgcttc gaacgcggtt atagtctcgt
     4381 cctttgcaat accgaaggcg atgaacagcg gatgaatcgc aatctggaaa cgctgatgca
     4441 aaaacgcgtt gatggcttac tgttactgtg taccgaaacg catcagcctt cgcgtgaaat
     4501 catgcaacgt tatccgacag tgcctaccgt gatgatggac tgggctccgt tcgatggcga
     4561 cagcgatctt attcaggata actcgttgct gggcggagac ttagcaacgc aatatctgat
     4621 cgataaaggt catacccgta tcgcctgtat taccggtccg ctggataaaa ctccggcgcg
     4681 cctgcggttg gaaggttatc gggcggcgat gaaacgtgcg ggtctcaaca ttcctgatgg
     4741 ctatgaagtc actggtgatt ttgaatttaa cggcgggttt gacgccatgc gccaactgtt
     4801 atcacatccg ctgcgtcctc aggccgtctt taccggaaat gacgctatgg ctgttggcgt
     4861 ttaccaggcg ttatatcagg cagagttaca ggttccgcag gatatcgcgg tgattggcta
     4921 tgacgatatc gaactggcaa gctttatgac gccaccatta accactatcc accaaccgaa
     4981 agatgaactg ggggagctgg cgattgatgt actcatccat cggataaccc agccgaccct
     5041 tcagcaacaa cgattacaac ttactccgat tctgatggaa cgcggttcgg catagattta
     5101 cgctgtcttt tgatcaaatt attaccatcg gttgttttca gaagcatgaa cattgctgct
     5161 gaagcaacag taataatgcc catcgtgata aacgtatagt ggaattgttc gacagtcgtt
     5221 gtgccttcca tcccttcata aatgcgaagg acggccgcac ttacagcaac gcctaaacta
     5281 atcgacagtt gctgcgtgac cgccagaaca ctgttaccgc tgctggcgtt gtcatcggtc
     5341 agatcggcaa gtgtgatggt attcatcgcg gtaaactgcg tcgacatagc catccctaat
     5401 ataaacaacg gcaagatcag catccatatc gccattgccg gtgattgcaa agagaactga
     5461 gcgatcatta gcccaataat caccgtgatc cccactaatg tatggcgata gcccagacga
     5521 cgtaagactt gggtaaccat cgattttgca ataatggaac ctaacgctgt cggtgccatc
     5581 atacagccgg caataaacgc ctgataacca aatcctacct gtaacatcaa tggcataagg
     5641 aacggtacac agccggtccc cagacgggtt gcaatattgc ctacgatacc gatcgagaaa
     5701 gtgcgggttt taaataaatc taatgaaatt aatgggtttg gcgtacgtcg cgcatggaga
     5761 atatagagaa gcagtaaccc gatgctggtg acaattaccg tcaaggcaat ccagctggcg
     5821 acaatctttt ccccgaatag ctctattccg cttgaaaaga gaacaaggct gaggccaaac
     5881 agcaaaaagc cagtgatatc gaatctgcgt cgtgcggtgg tgaaattggg catatgtttg
     5941 cgcgcgtaaa gaaggcccgc aatacctatg gggatattaa ttaaaaatat ccagtgccag
     6001 gttgcccagg tgaccagcac gccgccaaga acggggccta aaattggccc caccagaccc
     6061 ggcatggcga caaaattcaa taccggaaga agttcattac gaggataagc acgcagtaag
     6121 gccggccgag caacaggcat catcattgcg tcgcctatcc cttgaataac ccggaagaca
     6181 accagctgtg gtagcgaatt agaaagtgcg caggccagag aacccaacgt gaacagactc
     6241 acggcaaggg taaaaatgcg acgcgtaccg aagcgatcgg ctagccatcc gcttaccgga
     6301 ataagcatcg ccaccgtcag cgtataactg atgatggctg attgcatcgc gagaggagaa
     6361 cgattaaggc tatgagcgat tgcgggtaag gcggtattca gaatagtggc atcaagtgcc
     6421 tgcatgaaga aggccatcgc cgcgatccac ggcaaacccg acatactacg cttcttttta
     6481 tcgctcattc aatgtcctgt tatcgggtta tcacttatca ggtgagcgta gcagcgcctg
     6541 acaagcttta aatgccgcgt cgccatcgct ttggataatc gcatcgacaa tcgcctggtg
     6601 cagatccagc tttatcactg tgtcgctggt aattgacgtg aagtaagtgt gataaaccga
     6661 atggaatagc gaggcgaatg aggtcaaaaa cggattggcg ctcatttcat agatatgctc
     6721 atgccaggcc atatcgactt cgatccagcg ttcacggcga aagttctctt ttaatgccgc
     6781 catttcggcc attaacgtat tgagatgcgc cttctgttcc gcggtgccaa ccgttgctgc
     6841 cagtaggcag gcttgcggct ccagacagat acgcataacc agaaagtgat cgatgacctg
     6901 atgaaagttc tcttctgtta tccaccaagt aagcaattcc tgatcaagaa aattccagtt
     6961 cgattgtggc atgacccgag taccaattcg cggtcgcggt aaaaccatcc cttttgccgt
     7021 taacgttttg accgcttcgc gtaccgctgt acgactcact ccaaattgct cgcccagctc
     7081 aatctcaccg ggcaaaatgg tgccgggttc atattcacct tttaagatcc gttgcgccag
     7141 cttctcagcc agaacatacg aaaggttttt ctgtgcagct aactgttgtg cgcttaaagg
     7201 cattacttat cttccttttt ctttttattc ctccttagta tgccaccagg aagtgtgatt
     7261 acggttgcaa aaacggcaaa ttgcttgttt tatggcacat taacggggtg ttttgctgaa
     7321 aaaatgcgcg gtcagaaaat tattttaaat ttcctcttgt caggccggga taactcccta
     7381 taatacgcca ccactgacac ggaacaacgg caaatacgcc gccgggtcag cggggttctc
     7441 ctgagaattc cgacagaaaa agcaaaaata aatgattgac tctgtagcgg gaaagcgtat
     7501 tatgcacacc ccccgcgccg ctgagaaaaa gcgaag
//
LOCUS       DAJTUI010000152         7187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTUI010000152 DAJTUI010000000
VERSION     DAJTUI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.152"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(14..707)
                     /gene="ospZ"
                     /locus_tag="OO464_003747"
                     /pseudo
     CDS             complement(14..707)
                     /gene="ospZ"
                     /locus_tag="OO464_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            763..915
                     /locus_tag="OO464_003748"
                     /pseudo
     CDS             763..915
                     /locus_tag="OO464_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2296..3243
                     /gene="icsP"
                     /locus_tag="OO464_003749"
     CDS             2296..3243
                     /gene="icsP"
                     /locus_tag="OO464_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR6228739.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3805..4177)
                     /locus_tag="OO464_003750"
                     /pseudo
     CDS             complement(3805..4177)
                     /locus_tag="OO464_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4421..4540
                     /locus_tag="OO464_003751"
                     /pseudo
     CDS             4421..4540
                     /locus_tag="OO464_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4790..5656
                     /gene="ospB"
                     /locus_tag="OO464_003752"
     CDS             4790..5656
                     /gene="ospB"
                     /locus_tag="OO464_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR6228740.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5985..6725
                     /gene="phoN2"
                     /locus_tag="OO464_003753"
     CDS             5985..6725
                     /gene="phoN2"
                     /locus_tag="OO464_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR6228741.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7085..>7187)
                     /locus_tag="OO464_003754"
     CDS             complement(7085..>7187)
                     /locus_tag="OO464_003754"
                     /inference="COORDINATES: protein motif:HMM:NF017898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR6228742.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2251 a         1356 c         1385 g         2195 t
ORIGIN      
        1 caattaatat acattaagta acaggcattc gagccttgat ttgagaaagc ctgtcattca
       61 tttgttttat gtagctatct atttcctctg atgcataact aaaaagttct cccatttcac
      121 gagcaataat aatctcagat ttaatagact ttaatctctg gcgactcatc tcttaacact
      181 tgcataatct ctctttttat atcatatagt tctataaacc gatttgcccg tagttctcct
      241 tgggaagaat catttctgaa tacaacaatt atatacaaaa aatcactatt ttctttagag
      301 aagatcttac aactaatctc tgggagctca cataatgcat gtaagtcagg atggtacaaa
      361 ctatccacag aaaattgccc cataggacgt tttccagata taactgtcaa ccagcttcct
      421 tccggtgtac tggcttcggc tctggaaata ggctgaaaat cttccggaca tggagaatac
      481 attgtcttca tataattaag tcgtctggat ttctcattaa taaactcctc tatttttaat
      541 tgtgccatac ttctggttat accagagata tacttcttgt catatatgtt tggtgattca
      601 ttaaagtaac catgttcaac tctaggataa atatttctca ctatatattc agtatttgca
      661 gtatttattg gaaatacgtt cttaatattc ttgatgggac taatcatttt aatctctata
      721 ctcaaatata aacattacca tgaacaatat aattgttcct aagtggaatg tctccacgga
      781 gaacacttta tctatcggga aatagtgcgg gcaacagtgt ttaattatat cgagtgcaat
      841 tacaatcgct ggcgacggca ccgttggtgt ggcggcctca gtccggaaca atttgaaaac
      901 cagaacctcg cttaggcctg tgtccatatt acgtgggtag gctcacagag cctcacttac
      961 gactgcgtcc atattatgtg ggtaggatca ataaagacgt tttatatgtg tctgcttgca
     1021 gccagaaaat ttataccctg caggtatcat aaacatgctt gataacttaa ttggggctcc
     1081 ctcttccttt tatggtgtaa ttgtttgatt gaatacttcc ggggatttca gtatgaaatg
     1141 aagtatattt aatatacttt acaaaaagtt tgtattagtg gaataaggac aatagcttat
     1201 ccaaactgcc aagcctcttt attataagta agatctggca ctggaatatt ctcaaaagga
     1261 ctaacatcat caccacgacc ttcaaccaac cattttattg catttaaatc ccagaatagt
     1321 tcagtagtgg tcaatttggg aaacgcacaa tattgtacgg ttacgttatc gcaatagagt
     1381 gaactctcgc cctatacggc gttttcaagg attagtcctt aatcggacaa ccacattaaa
     1441 gcataataaa taattaagct ttaataatat actatagata cagttctatt ataaaaaata
     1501 ctattttagg tgatgcatta aattagtaga atatttattc ttttataaaa gcaccaatga
     1561 attgtccaag taaacatgag ataaaatgtg tagaggataa ctgagtttgg tcactttaac
     1621 tgtattagtc gcagtctgac tctcgacttt aaaaggatgg gaattacctg tactgatagt
     1681 gagaaagctt tccattgaaa taagtcaaat aaaagttgat ggcttcagtg ctaaagtgag
     1741 gtgcccgcta gccgtattca ctcgaatcgg aattcaatgt attgcttctg ccatttccat
     1801 aatatttcac gggaaaaaca aaatctgaaa aactatactt aagtgttgta ttgagtcagt
     1861 tgagatacaa atacatattt atcagcacac gttacgtcaa atacaggagg ttgtcatgtt
     1921 ggtactgaaa ggcacgttgg ctattgcagc cgtgatgaca atttattgtc ttatcgttgt
     1981 tcttatggaa cgcttttctg attgatttca tattggcgag agaacaagaa ggtgagtaga
     2041 attgtcgcgg aatcctgaaa ctatcagcct caggtatgaa caccaaaagg taggtagaga
     2101 aatctatggc ccccgttact ttattcgcac aatgaggtat ttttttattt ttacaaaagt
     2161 tcaataaata ttataataaa agatatgttc ttggtaacat aaacaaatat ttaattaaaa
     2221 taatgcttgg cattgacgtt tatcttataa agtaagaaga tcatggacat ttcaaccaaa
     2281 aaagtagagt tctcgatgaa attaaaattc tttgtacttg cactttgtgt acctgcgatc
     2341 tttactacac atgctaccac taactatcca cttttcatac cggacaacat cagtacggat
     2401 attagtctag gatctctgag tggcaaaaca aaagaacgcg tttatcatcc caaggaaggg
     2461 gggagaaaaa ttagtcaact ggactggaaa tacagtaatg cgactattgt tagaggtggc
     2521 atcgattgga agctaattcc aaaagtgtct ttcggagttt ccggttggac tactttaggt
     2581 aaccagaaag caagcatggt tgataaagac tggaacaatt ccaatacccc tcaggtatgg
     2641 accgaccaga gctggcatcc caatacgcat ctccgtgatg ctaacgaatt cgagctgaat
     2701 cttaaaggtt ggttattaaa taatttggat tatcgacttg gactaatagc aggttaccaa
     2761 gagagtcgtt acagttttaa tgcaatggga gggagttata tttatagtga aaacgggggg
     2821 agcaggaata aaaaaggggc acatcctagt ggtgaaagaa caataggtta caaacagctc
     2881 ttcaaaatac cttatattgg attaactgct aattaccgcc atgagaattt tgagtttgga
     2941 gcagaactga aatatagtgg ttgggttctt tcatctgata cagataaaca ctatcagact
     3001 gagacaattt ttaaagatga aataaaaaac caaaattact gctctgttgc tgcgaatatt
     3061 ggatactatg tcacccccag tgcaaaattt tatatagaag gctccagaaa ttacatttct
     3121 aataaaaaag gtgatacatc tctttatgag caaagtacca atatatctgg caccattaaa
     3181 aatagtgcaa gtattgaata tattggtttt ctcacttccg caggtataaa gtatattttt
     3241 tgaattggtt caccgagttg attacgtttt catggctaaa aattgccgcc cattgtgagt
     3301 catattcgcc ctgactttcc agaaccatac gaactgcccg ttgacggact tcgggggaaa
     3361 aacgtgtatt tttagtcatt ctgtttacct ctttctcagg gcgtttagtc tccaggattt
     3421 ccggggcggt tcaaaaaact cttcgttgat tactgtggac ttactgttgg cattaccgac
     3481 cctgagaccg gagaaataag aaccgcttag atcatcgtgg ctgttctcgg ggtatcaagt
     3541 tacacatggg cagaggctac ccaaggtcta ctattatcgc ctgaaagcgc tgatggaaca
     3601 gtgctatcag ggacatgctg atgaaaatac ctaaataatc agccagcact ctatctttcc
     3661 aaatccacag catagcaaag agagcaaaag agcctgtaaa ttcagaaatt aaggaaacgc
     3721 ctcttggtgt acataatcta tcttatgtta aatgaaatct aatttcttga ttatatgctg
     3781 gatcgttcgt gggtggcttt gcccctatat ttccagacac ctgttatcat tggcccgctg
     3841 ccgcagatat tcccgtggcg agcgataatc cagcgcacta tgcggatgcc attcgttata
     3901 atgctcgaat gcctctgcaa ggttctttgc tgccgttaac ccgtcaggtt tgggcatgat
     3961 actgatgtag tcacacttta ttgttttcac gaagctcttt gctattctgt tactctccgg
     4021 actccgcacc gccgtgttct tcggttcaac aaagtcccaa catccgggtg aactggcgtg
     4081 tttcattagc tcggtagcat gaaccattat ccgtcagtca cttcactgga gacgccggaa
     4141 gctcgttgcc gaagcggtgt tccaccgctc ccagcatgtc gccaacggga gagcatctac
     4201 ggtctgattg aactgtttat tcaaattgtc caccgtctgt cagtacgctc tgaacgtcgg
     4261 ctggttaaaa cactgctcgc tgattttcag agggttcacg gtaaaacagc acttttattc
     4321 cggattgcgg aagcggctct gaataatccg gatgggctgg ttaaagaggt tgtgtaccat
     4381 ctgcatattg ttgaaccaga tcgcagcggt aagagaagtt catcatactt ggcgcagtta
     4441 cgcgatgttt ctgctagagg tgatgccgta aagaacggca gaacattacc ggaacaagat
     4501 agcggtctac ctgcactggt atcagacccg gggcttcccc ggatgatctc cacggtactt
     4561 taggtactgt gtctatatta tgtaggtagg atcaattaac tttgtagttg aaattctttc
     4621 attgaaataa tgaataaaaa agacaaaccg cattaaacaa tgccagaata taattgcatt
     4681 tttgttccgt ttttttaaat catagtatac aaagaagctt gcatactata tgaaaatata
     4741 acacccttta ctatgatgtt tataaaacaa tatatggagt catgtaggta tgaatttaga
     4801 tggtgttaga ccatactgta gaatagtcaa taaaaagaat gaaagcatat cagatattgc
     4861 atttgcacat ataataaaaa gggtaaaaaa ttcatcatgt actcacccaa aagcagcatt
     4921 ggttttttta ggagagaaag gtttttgtga tagcaatgat gttctatcta ttatgggaca
     4981 acaaatacca agagtattta agaacaagat gttatatgat tatgttttta aaaatgaaaa
     5041 aagtaaaaat gattttctaa aaatggctga atcatggcta ccacagagtg aaccaatagt
     5101 aataaataat gatgatgacg cattgaatgc tgctgcttat ttttctgtaa aaaaagcgaa
     5161 aataaaaaca gtaaacgata ctgattttaa agagtataat aaggtttata ttcttgggca
     5221 cggtagtcct ggttctcatc aattaggcct tggttcggaa cttattgatg tacaaacaat
     5281 catttcaaga atgaaagact gtggtattct aaatgtgaaa gatatccgtt ttacttcatg
     5341 cggctccgct gataaagtgg ctcctaaaaa ttttaacaat gcccctgctg aaagtctttc
     5401 ttgtatcctt aactctctgc ctttttttaa ggaaaaagaa tctttgctag agcagataaa
     5461 aaaacacctt gaaaacgatg agtcattgag tgatggtcta aaaatatccg gctatcatgg
     5521 atatggagtt cactatggtc aagagctttt tccctactca cattatcgtt caacttcaat
     5581 tcctgctgat ccggagcata cagtaaaaag aagctctcag aaaaagactt ttattattaa
     5641 taaagaactg gattagtata aaatttttaa cctatagtta attgtttgca tttaagatga
     5701 agttatttta tctttagtag aaaagtgctt ccctatgagg gaaaatcaca aatcatcata
     5761 atacagagac aaaacgatac gaaaaaatag ataaaaaaca tcattctttt accacattat
     5821 tttccgtgaa tatgaaaaat aatgttatta ctttaatata agactatttt ttgtttttcc
     5881 atcactctgt tcaaattttt ccgcatgact tgtgtttttt gtaatacagc tcgtttttta
     5941 cagctgacca aaatcatcaa ttaattatgc taaggaaata aattatgaaa accaaaaact
     6001 ttcttctttt ttgtattgct acaaatatga tttttatccc ctcagcaaat gctctgaagg
     6061 cagaaggttt tctcactcaa caaacttcac cagacagttt gtcaatactt ccgccgcctc
     6121 cggcagagaa ttcagtagta tttcaggctg acaaagctca ttatgaattc ggccgctcgc
     6181 tccgggatgc taatcgtgta cgtctcgcta gcgaagatgc atactacgag aattttggtc
     6241 ttgcattttc agatgcttat ggcatggata tttcaaggga aaatacccca atcttatatc
     6301 agttgttaac acaagtacta caggatagcc atgattacgc cgtgcgtaac gccaaagaat
     6361 attataaaag agttcgtcca ttcgttattt ataaagacgc aacctgtaca cctgataaag
     6421 atgagaaaat ggctatcact ggctcttatc cctctggtca tgcatccttt ggttgggcag
     6481 tagcactgat acttgcggag attaatcctc aacgtaaagc ggaaatactt cgacgtggat
     6541 atgagtttgg agaaagtcgg gtcatctgcg gtgcgcattg gcaaagcgat gtagaggctg
     6601 ggcgtttaat gggagcatcg gttgttgcag tacttcataa tacacctgaa tttaccaaaa
     6661 gccttagcga agccaaaaaa gagtttgaag aattaaatac tcctaccaat gaactgaccc
     6721 cataaagctg gacagcctgt atcaggctat ggagggccca tagacaaatc taccctatat
     6781 gagcatagga ggagtctatg ggcacaccac gttttacccc tgaatttaag ggattactgg
     6841 aaaggctggg acatatccct ccggcagaag cagaaaaagc ttattatgct gccatcggaa
     6901 acgatgatct ggcaacctga gttcacagat aaaacattct ctaggaaact cggggcggtt
     6961 ccgttcacca catgcaatgt ggtgttgcag gggaacggtc tgcaccatcc cctatgtcga
     7021 tcaatataac agaaatgaca acttcagatt cagggcacaa cctaaatata ttttaggtca
     7081 cctctcaaat cgtttgcctg ataccgctcc attttttaat aaaaaaatca atcatttttg
     7141 aatctttgct tttcattgca ttgtcaagca tagtgtcccc actattt
//
LOCUS       DAJTUI010000153         7125 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTUI010000153 DAJTUI010000000
VERSION     DAJTUI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.153"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            41..1531
                     /locus_tag="OO464_003755"
     CDS             41..1531
                     /locus_tag="OO464_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6228743.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1583..2740
                     /locus_tag="OO464_003756"
     CDS             1583..2740
                     /locus_tag="OO464_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6228744.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2706..2828)
                     /locus_tag="OO464_003757"
     CDS             complement(2706..2828)
                     /locus_tag="OO464_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228745.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3031..4716
                     /locus_tag="OO464_003758"
                     /pseudo
     CDS             3031..4716
                     /locus_tag="OO464_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4754..7114
                     /locus_tag="OO464_003759"
     CDS             4754..7114
                     /locus_tag="OO464_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6228746.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         2101 a         1556 c         1510 g         1958 t
ORIGIN      
        1 atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc ttggttatgg
       61 acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc gtgaagttgt
      121 cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat caacgccgac
      181 gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg cacacggtgt
      241 ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct ttggtgtcta
      301 ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc ctgaattatt
      361 ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa aaatcagtaa
      421 tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca ccacattttc
      481 tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat tccacgctgc
      541 aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg tccccgcaaa
      601 aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc gtgccaaaac
      661 acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac caaatgataa
      721 tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag cagataacta
      781 ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac aactgaagaa
      841 aaacggacag tatgacaata caattattct ctttacctcc gataatggtg cggttatcga
      901 tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct atcctggcgg
      961 tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta attatgacaa
     1021 gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata tcagcattcc
     1081 aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata agaaacaagg
     1141 cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg atgaggaaaa
     1201 tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg attacccgca
     1261 taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata acgattattc
     1321 gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg atctacagca
     1381 aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag gcgtggtaag
     1441 agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga agtttaacaa
     1501 tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg atttttccgc
     1561 cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt ttcaatgtaa
     1621 tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc atgaaaaatg
     1681 gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt ctggcaatca
     1741 ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt ttttccgtaa
     1801 agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata cattacaaac
     1861 gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg aatttctggt
     1921 tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca gtaattcagg
     1981 taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat atcaagtaga
     2041 gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg aggtttatca
     2101 ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag aaaccgggac
     2161 gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg atttttctgt
     2221 gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg ttaaaaacga
     2281 tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt tggggaatgg
     2341 gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg aaagtaatgg
     2401 agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa acattaataa
     2461 atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac ggatttcagc
     2521 gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta agcatcgtat
     2581 tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa tccttttttc
     2641 aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag taccgcttta
     2701 ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc ttttaactcc
     2761 attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc tgtcgggtgt
     2821 ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg tcagggcatt
     2881 agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa aatttctata
     2941 tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt gccatagata
     3001 atcattggat ggcacaatca attttcgcta atgataacca ttcccattac gttttgtatg
     3061 ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa taataaaact
     3121 tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa aattcaggtc
     3181 tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga aaccgataag
     3241 ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt aatatccgca
     3301 aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt aacagattac
     3361 taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta cggcgagcat
     3421 aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact gatcaacaaa
     3481 acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac ctttaccgtt
     3541 attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga atggaatatc
     3601 caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt atttacccat
     3661 aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga agcgacattt
     3721 cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag caatgctgag
     3781 tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa ctggcatcgt
     3841 ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg ctcgcttagc
     3901 gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa tctgggcgga
     3961 ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg gtttatttat
     4021 aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga gttccatcaa
     4081 ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt acaagtggct
     4141 aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa ctttcatgtt
     4201 tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac cacactgctt
     4261 aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc tgctgacagt
     4321 tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat tatttgtaaa
     4381 gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca ggttggtctg
     4441 ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct ttccagcggc
     4501 gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg gatatttctt
     4561 gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg tttagtgcgt
     4621 gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt ctggaacctg
     4681 gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc ctgctgtacg
     4741 gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg gcgctgatgt
     4801 ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga tgacggtcta
     4861 tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa gcatcgagcg
     4921 tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg cggtacgcat
     4981 ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga aaatctctat
     5041 tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg caactaataa
     5101 cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg ggatgtcaca
     5161 ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca gttttgtgcc
     5221 ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac gcttcaacgc
     5281 tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt taacatcgca
     5341 tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta cctatttctc
     5401 tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg ctgataactt
     5461 tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg attatgtttc
     5521 gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata ctgcattgag
     5581 caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa aatataccgg
     5641 ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa tgggtaataa
     5701 atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac tacgtaccac
     5761 cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct ggtacaccga
     5821 actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat taggacacat
     5881 ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa aattcgccgt
     5941 gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg atgcatggac
     6001 tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga aaactaacca
     6061 taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc tgtatatggc
     6121 ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg actatgacaa
     6181 ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata tttttgctga
     6241 tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata ttcttgatat
     6301 gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata aaaccctgac
     6361 gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat tgcagcaaaa
     6421 aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca gcaaaagcag
     6481 tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg gcaaaaccaa
     6541 aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc gccaggtaga
     6601 tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt cgttaaacaa
     6661 tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta atctggtctc
     6721 ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct taaacatgga
     6781 tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc aagaagcgcg
     6841 taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat attcagatta
     6901 caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc gcttgtcctg
     6961 gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc tcaatgtact
     7021 ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg ccagtggccg
     7081 tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggatt
//
LOCUS       DAJTUI010000154         7065 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTUI010000154 DAJTUI010000000
VERSION     DAJTUI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.154"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(1..494)
                     /locus_tag="OO464_003760"
                     /pseudo
     CDS             complement(1..494)
                     /locus_tag="OO464_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            637..759
                     /locus_tag="OO464_003761"
                     /pseudo
     CDS             637..759
                     /locus_tag="OO464_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1021..1587)
                     /gene="ipgB2"
                     /locus_tag="OO464_003762"
     CDS             complement(1021..1587)
                     /gene="ipgB2"
                     /locus_tag="OO464_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR6228747.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(1869..2183)
                     /locus_tag="OO464_003763"
     CDS             complement(1869..2183)
                     /locus_tag="OO464_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228748.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(2261..2371)
                     /locus_tag="OO464_003764"
                     /pseudo
     CDS             complement(2261..2371)
                     /locus_tag="OO464_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2695..3372)
                     /gene="ospD1"
                     /locus_tag="OO464_003765"
     CDS             complement(2695..3372)
                     /gene="ospD1"
                     /locus_tag="OO464_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR6228749.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(3744..3881)
                     /locus_tag="OO464_003766"
                     /pseudo
     CDS             complement(3744..3881)
                     /locus_tag="OO464_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(3963..4261)
                     /locus_tag="OO464_003767"
                     /pseudo
     CDS             complement(3963..4261)
                     /locus_tag="OO464_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4850..5009)
                     /locus_tag="OO464_003768"
                     /pseudo
     CDS             complement(4850..5009)
                     /locus_tag="OO464_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5013..6242)
                     /locus_tag="OO464_003769"
                     /pseudo
     CDS             complement(5013..6242)
                     /locus_tag="OO464_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6262..6609)
                     /gene="tnpB"
                     /locus_tag="OO464_003770"
     CDS             complement(6262..6609)
                     /gene="tnpB"
                     /locus_tag="OO464_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6228750.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(6609..6983)
                     /locus_tag="OO464_003771"
                     /pseudo
     CDS             complement(6609..6983)
                     /locus_tag="OO464_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1799 a         1651 c         1535 g         2080 t
ORIGIN      
        1 cgccagcgat tgtaatcaca ttcgatataa ttaaacactg ttgccctcat tatttcccgg
       61 ctgataaagt gttctccgtg gagacattcc actttcagcg aatgaaagaa gctttccacg
      121 caggcattat cgtagcagca gccctttgcg ctcatactgc cacgcagatt atgccacttc
      181 agcagcgcct gataatctgc tgaacagtac tgacttcctc ggtccgagtg aacgataacg
      241 ttccggggtc tcttacgccg ccacagcgcc atctgcaggg catcgcaggc cagttgcgcc
      301 gtcaggcgtg gtgacatgga ccagccaata acggcacgtg accacaggtc aatgaccaca
      361 gccagataca gccagccttc atctgtacgt aagtacgtga tgtctcccgc ccacttctgg
      421 ttcggaccac tggcgtaaaa atcctgctcc agcagatttt ctgacacagg caggccatgt
      481 gcgcggtaac tgaccgtatt gctgggtaag atcaatttgc tcactggtat ttactacgtg
      541 catcgaatga gtgatcaaga gccgtatgac gcgagagtgt cacgtacggt tccgtaagca
      601 gccggagagt gaaactctct ccggctgact cgactcgcag gttccgacag cggtgacgaa
      661 catgcagcgg tgttgttctc gctgatcggc acatgccgtc tgaacaatgt ggagccagaa
      721 aaatggctgc gttacgttat tgaacatatc caggactgac cggcaaaccg ggtacgcgat
      781 ctgttgcctt ggaaagttga tctgacttct cagtaaatat caatatgatt ctgacgagcc
      841 gcttaccttt gagcagagaa aggggagagg gatcccacat ccatgagcta ggaaaataca
      901 aaaactggct atacaacaat tcaatatatt aggacttact tgcgaggaga aatatagcac
      961 cgctcaggca attcctgggt atttactttt cttagtttct ttactcggga tgttctgtca
     1021 tcagaaaggc gattctaaat ttgtaatata gtcactcata ttcccacgta atttctttag
     1081 atcaatatat tgcacatttt tctcaaaaaa ctgatcggaa ataagatttg caacattgct
     1141 tgttgtatca tttttaacgc ttctattcat cccttcacaa agttcgtcca ttttcttttt
     1201 gaaataactg cttcctgata caagatgatt tatggaactt tgtgcagcat tgatgtctag
     1261 agaaatgcga taatctttct caacctgttt aaatatctgt ttacggagat tatcgccgat
     1321 ttttcggctg tgttctgcac aaaacttatc aatacattgg tttaccaccc gggatacgta
     1381 gacttttttt tcatcatttt tccaatctgt aatattttca ccgatttgtt ctgaaacaga
     1441 gctcaaaata ctagtattta ttttggttga tgatattttt actatccttt tccccattgt
     1501 acagcggata atctcatcca cctttcctga aaaatatctt ttgtgagata aactgattcc
     1561 aaaattatta aaagatgttc caagcattat aatcaccttg tcatgtaaaa tagtaacatt
     1621 gtcattattt tagctgattc aaacaattct gtaaatgctt tgagttcttt actcccttgg
     1681 ctcaatgatg gaccgtctgg aatcccagag actaaactct ctgagaaaga gagtatgaaa
     1741 aaacttgggg cagtccctaa atgaagggac caacagcggt aaaataaccg gcatcattca
     1801 taatgatgcc ctgttagttg tagttatgaa gatttacgtc attaaaattg ctgttcgtag
     1861 tgtcagtcct atggtctggc atcagctgag gatcgtagcc gatacatcac tggcttcacg
     1921 acattcgtgt tgaggctatt catgaaaact ccacgctgaa aacgccgttt tgtataaacg
     1981 gtcacggtat gcccggagcc actgttgctg ataaattcga taaaaccttg gcgtttcttg
     2041 aagctatcgt taatgcggat aatgaaacaa cgattggtga gattcgctct tttgccgacg
     2101 ccctggacgc tgtccggttc aatcgcaaca aaatcaaccg gcagctgagc aaacccaacc
     2161 ttgcctctct ggcgctggag catgaagtga tttggctggg acgtagccgc tgacagcttc
     2221 tataaaagta tgatcctacc cacgtaatat gaacacaggc ttaagtgagg ttctggtttt
     2281 caaattgttc aggactgaga cctccacacc aactgtgccg tcgtcagcga ttgtaatcgc
     2341 acttgatata attaaacact gtatatttca tttgtggagt ctccagtctg agagggcttt
     2401 atgcattccg tattgggcac tatgatgcca gaaatctgca aggctccaca ccttcctgta
     2461 tttcccttcc tctatcacag tgcactttac ttaatagggt gggatgtgtt cattacattt
     2521 attgtgtttt ttatgattgt ttttgcgtcc agtgtcgttc gtcatgggaa actggttcta
     2581 tgggtagtat cgctcagtct agttaactgt ttgtttcttt ttagaaatta agcagatcac
     2641 acaattcggg gagagatcct tctaagtgca attctttgca gctatttagc acacttaaaa
     2701 caactcttgt attttgtcag aaataatatt aaacatgtct tgttcattat tgttaagtct
     2761 gtctgctatt tctgtaagtt ttatcccact atgatggcat atatttatat atgccatcat
     2821 agcctcgata ttccctttcc ctgcggccag aaataatcct ggtaacccct cattatctct
     2881 tacatccaat agtttaatcg tttcttttgg tgataaatca aaagttttaa taaaatctcc
     2941 ataagcttga atcgcagcgg aatgaccttc ttgaagagct atgaacaaag cagattcccc
     3001 agcattattt cgtgctgcaa gaacttctag tttttcctct ttgggtattt cggctgccaa
     3061 cagcgattca ccgtatttac gcgctgcatc ggcatttcca tgccaaatca atgaaaacaa
     3121 attatggcgg ttatactcct tattattctt tgtcggaatg gatatgttct gtattttggc
     3181 caattttctg aaagaaacac cactatatcc cccccctgat ttttcttcat tgatggcgtg
     3241 ggatatagcg gtatttgtca cgttaatgat attatttttc attcctttat tttcattagc
     3301 agtattgctg cctattatta agtgcatatt tttgttgttt gctggatgta atccatagtt
     3361 atttattgac atttttgttc atcctttttg ctaaaaaaaa acaataacct atttttgtaa
     3421 tgtaatagat attagtctat tgctaatcta aaaaaaccat tgccacattg aatggctata
     3481 ttcaccaata aattctttcg atttcgtata ttgacatatc gtagatatat cttatcccta
     3541 agattcaata atcgagtccg tttaccattc tgtgcaagcg ccttcactca gaagtgaccg
     3601 tcaaggcgat caccaaactt gataataaag cagctcattg ccatacgcca gtccttaagg
     3661 ggcgttgtcc atttctgtga tgctgcctag atttaactgc cagctccggc ttatctacat
     3721 ctaaatatgt gagcctaata ccgctacact ttgtccagac tatgtttttc tccgggaagt
     3781 gggctaaccg tctccataaa gtgacgaact tttttcaaca gtggccatat tgaacggcac
     3841 tgatgattac gggttattgt ctcatgaaga gccggccaca tcactgacag cagtgacttc
     3901 aggccttcag caccagacag cctgaaccag ttaatccatc gcccgacaaa tttagtaatc
     3961 tagggctggt ttttcttcag ctgttgcggt tcgaaagtgc cattacgatc acgcagagta
     4021 cggatttcca gaggttcatc tccagtagtg ataatcttcg ttaagcaacc attgcgggaa
     4081 ttgctgccag atctaagctg atttttctcg cacccaagat gatgagtcat attagcattg
     4141 agtacggttt cattttcagc atccaatcaa actgactgag atcttcagga tttttgagac
     4201 ttttggctag ttcattagct aaagcccgca acttattaga tgtcaactgt ttttcattca
     4261 taaattcacc ttcatttgat atcaaattaa atagttcaac atcagagaat tatacaaact
     4321 tatgtacagg ctcctatgat cttcgaatcc tgaagattat agtgaaaata agttttcacg
     4381 ttgaaatccc acatagttag tatatgataa ataatcatgg atgacctgat taacctcttg
     4441 gggtagtgct tttatagaag aataaatatt ctactaattt aatacatcac ctaaaatagt
     4501 attgtttttt atggttggtt gaaggtcgtg gtgatgatgt tagtcctttt gagaatattc
     4561 cagtgccaga tcttacttat aataaagggg cttggcagtt tggataaact attgttctta
     4621 ttccactaat acaaactttt tgtaaagtat attaaatata cttcatttca tactgaaatc
     4681 cccggaagta ttcaatcaaa caattacacc ataaaaggaa gagggggccc taattaagtt
     4741 gtcaagcatg tttatgatac ctgcagggta taaattttct ggctgcaagc agacacatat
     4801 aaaacgtctt tattgatcct acccacataa tatggacaca gtcgtaagtg aggctctggt
     4861 tttcaaattg ttccggactg agaccaccac accaacggtg ccgtcgccag cgattgtaat
     4921 cgcactcgat ataactaaac actgttgccc gcactatttc ccgatggata atagtgttct
     4981 ccgtggagac attccatttt cagcgaatga attttcatct gttgctgtat ccagtcatac
     5041 agtgactgca tcagtggcgc ggctctggct tttcttgccg ccagacgctg ttctgctgaa
     5101 catccccgga cttctgcctc gatggcatac agttcaccga tacgctgcag ggcttccgtg
     5161 gtgatgtcgg ttggcactct tgcatgcaca tcgtggattt ttctccggac atgagccata
     5221 catgcggctt ccgttattct gccggattcg tacaacgccc ggtaaccacc gtaagcatcg
     5281 gcctgaagca ctccgctgta accggccaga tgattctgtg gatggatacc tttccggtcc
     5341 ggtgagtacg cgaaccagac cgccggaggc atttccgaac cggcgttacg gtcatcacga
     5401 acgtagaccc acagccgggc agtccttgtt ttaccgctgc ccggctcctg gaccggaacg
     5461 gggatatcat cggcatggac tttaccgggc atcagcacat actggcgcag gatgtcatac
     5521 agcggttcca gcagttcagc aacagcacct gtccagcgcc ccagtgtggc acggctcagc
     5581 tccacgccct gacgacggta tatttctgac tggcggtata acggcagata gtctgcatat
     5641 tttccagcaa caacatgggc cagaagcccc gctccggcat aactgcgtgc aatgggtttt
     5701 gaaggcaccg gtgcctgcac gatatggtca caccggcaac aggccagttt cggacgttgt
     5761 gtttcgataa ccttaaaggc gctgctgata agctccagct gctctgacac atcacatccc
     5821 agagaactga gttcgccacc acaggccggg cagcattcct cttccggacg gatgacccgg
     5881 gtttcacggg gaagtgaggc tggtaacggt ttacgggctg aagactggcg caggggagat
     5941 ggcagtaccg ggtcatattg ctcacccagc gtttccgcca tttcttcctg aagtgcgctg
     6001 attcgctcct gtgcttccag tatctgccgt tcggtttttg cgcgaagttt ttctgagctt
     6061 ttaccgaact gcatgcgctg cagtttcgca accagcgcct tcagccggtt gatttcggaa
     6121 gcataagccg ccacccgctg tgagagcagg cggttgtatt tcgccatctg gcggatggtg
     6181 tcctgttgcg tctgcaacag tgcccgcagg cgggcattct catgagcaag tgaggtgtcc
     6241 atatcctcac tttacaacgg gttatatgcg gatgccagcg cgttccgttc gtttcgggtg
     6301 tttccagtcg atgccctcaa gaagcatgga taactgggcc ggagtaaggt gcaccttgcc
     6361 gtcacgggtg accggccaga cgaagcggcc ccgctccagg cgtctggtaa agaggcacag
     6421 tccgtcactg tcagcccaca acacttttat ctggtcaccc cggcgtccgc ggaagatgaa
     6481 cagatgcccg gagaacgggt catccttcag gacgttctga acttttgaga ccaggccgtt
     6541 aaagccattt cgcatatcgg tgatacctgc aaccagccag atacgcgaac ctgcagggaa
     6601 agatatcatc agtggctgct cccttttatt tcgcggataa gtatctgtaa taacgccggc
     6661 gtcagtttac ctttaagcct gagagttccg gccggcagaa ccagctcaca gcacagactg
     6721 tcggacggtg tatttatctg ctctggttcc tgtgccggag ccgggatttt attatccggc
     6781 tccggcgtta acgtcacggg aagcagtgca ggcatatttt ttccggaagg cagcaggcca
     6841 cctttccggt attgatggcg ccagttgaag agcaggttat cgttgattcc gttttcacgg
     6901 gcgatctgcg ccacacaggc tccgggctgc agtgactgct ccactaaagc gattttaaat
     6961 tcataaggaa aattaggccg ccggtgtaat gccgatcagt taaggatcag ttgaccgatc
     7021 cagtggctgt gtaagaatcc ggaaacgctc acttgtttcc ggatt
//
LOCUS       DAJTUI010000155         6963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTUI010000155 DAJTUI010000000
VERSION     DAJTUI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.155"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(365..946)
                     /locus_tag="OO464_003772"
     CDS             complement(365..946)
                     /locus_tag="OO464_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228751.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(993..4850)
                     /gene="sigA"
                     /locus_tag="OO464_003773"
     CDS             complement(993..4850)
                     /gene="sigA"
                     /locus_tag="OO464_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR6228752.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(5211..6215)
                     /locus_tag="OO464_003774"
                     /pseudo
     CDS             complement(5211..6215)
                     /locus_tag="OO464_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            6200..6577
                     /locus_tag="OO464_003775"
                     /pseudo
     CDS             6200..6577
                     /locus_tag="OO464_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6616..>6963)
                     /locus_tag="OO464_003776"
     CDS             complement(6616..>6963)
                     /locus_tag="OO464_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6228753.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         1735 a         1567 c         1573 g         2088 t
ORIGIN      
        1 catcacctgc tattaatccc cgagtcagta cttcgcggcc tgacgcgatc accctactca
       61 gtacttcaga gcccgcctcc ggtacctgcc tggcgatcgc ccctgtgcaa caaaataaat
      121 caaccgttat caaaccacga tcgttcgtat gcgccgccga tgacgcgcgc ctgaaaaacg
      181 cgtcaatatg agttccccgg aagcttacga taatacgctt atgaacatta caacacgctc
      241 atgcggaacc tggatggggg aaggaacggt gaagctgtta gcgaatgacc ggcgtttata
      301 caaacgccgg tcatgatgaa tgatatttca gaatatacct cgggctaaaa taccgggaca
      361 gagatcaggc gtgattaatc tgcagcattt ttaataacag aagtactgag cttactttac
      421 ctttgatgat gtaatagtta tttccgtcgg tactcatttt tccctgaaat aatgtcacgg
      481 atgtttcttc ctgctgatcc tgcaggcgca gtcgtaaggt catattttta atgagctgag
      541 atccgggtgg caaggggtat ttgctgtcag atactgggcg actgagtctg atgtggcgcg
      601 tatacagccc gttttcgtac agggtggcct ctgttatggt gtcagtgacc agcatgtggg
      661 agacaatgct gtattctttt ttaaagcacc caccagaaac ggtgattgta atgataatgg
      721 tcttctcgat cgagatgtaa atacccgttt cgttatactg tccgtatcgg aaccatgaga
      781 actcagttgc attccagcca tgctccagaa tatgtgcatt tttttgcctg atagtttctt
      841 tttttattcg ctcatgttta aggcataaca ggcagataaa taatgtcaga aaaagaaagc
      901 taataatgag agcaagagcg atatagtctg tcacagggta atacatattc tctccggtag
      961 aagaagggcc gcaaacgcgg cccgggctgt tatcagaaag agtaacggaa gttggcgttg
     1021 atcgcgttat ccacgttgta tttaccaaat gccgatttct caaactcaag accgaagcga
     1081 agattatcgc gaatttcggc gttgagacca acattcatga gaatacgacc gtctttttca
     1141 cctttgatac gtttctcacc ggacgcatca cgcagtacgg tttcaccgtt ggcaaacagg
     1201 tcaaactggt agccaaggcc ggcgcgggct gtgactttcc agtccttacc ggagaaggat
     1261 ttacccacat caacaccggt acgcccaatc agcggattaa agtccttatc cttcatggtc
     1321 acgctcattc cgcgatcctg ccagtcgaac cgtttacctg atacggcccc ataaaccagt
     1381 tctgcctgcg gctcaatcca ggagtcttct gttacatgat agcggtagcc tgcttcagca
     1441 cccacataca aggaatgaga actgtagtct tttgttccga gtccagcaaa ggtcgccgag
     1501 tactcattat catggtgaac atacttacta atcaggtcga tataggcacc agagtcaaat
     1561 atagcggaag catacagacc tgccccgacg gacttcgtct tgccactgaa tgcattactg
     1621 ctggcatgac tgtcggtata cgtcatagtc agaccagtga aaaggtcaag tccatccagc
     1681 tcatgttttt tatccacacc aatctgcaca tgggtgtagt tgtcactgta tccaccagaa
     1741 gctgaacctg ctccgctcat gatgcgggcc catgcaccgg cctcgccatt ggtgtcacgc
     1801 agatccccca ttcgtttatt caggttgttg acttctgtca ggaaggcttt gtaattaccg
     1861 gacatatacg attttgctgc accggaagat gctgtatttt cagccacctt atatccggtc
     1921 agggtccacg tggtatgttc ctgatcctct ttcgtgctga tcacaggaga tatgttgctg
     1981 aaacctatgg cctgagtact ggctttaaat atctctttat tgcttccttt tggggctgtt
     2041 atcaggtcaa cgtttaatcc tgagttatta tttcccactt tctgtatatc gaccgacaga
     2101 ctgttattgc ttccggttaa ctgattttta acaatgagtt tatccgattg atttacagac
     2161 tggctcagat cagcccgcat aatcacagca gtattagtgg catccagtgt gtttactgtc
     2221 acagtatgga aacccgaatt ctctatgtta ctaaacaaca aaagactgtc gcgggttttt
     2281 aatgattgca gggctgactg tccgtttact ctccataagg tgttatacat gctggctgag
     2341 gctgcaggag catcaatacc tccggtataa gaagtatcaa agccacccag caaacctgta
     2401 gccagcggtg tgtgtgtggt tttgtccagg ttatcttctt tatactggtc aacaccaaac
     2461 agaatagatg cttttttgtc tgattttata ttaccattga cccaggcata attttgagcc
     2521 tttaacgtgg caccatcttc tgtcatatta tatccatctg acatatagat tgccgggctg
     2581 tatttattat tttctgtagg ggtgcctgtt aatgtcaggg ttccggccag ttcaatagct
     2641 ttatctacat acatttttcc ctgggcggta acagtagctc ctttgttgat aatcaggtct
     2701 gtattaatta aggataatgc accagtcata gtaacagttg atttatcacc aacagttatt
     2761 ttgctgtttt gagcactcag actgccgttg aaactacttt cgcctgctga atccccggag
     2821 cgaagctgtt gtctgaatcc aaaaccttcg ccagtaatgt ttcttcctga gaacatgtca
     2881 atgtatgcag ttttatttcc cagaacaatt tgagagtttt tagcctctat gtgtcctttt
     2941 acattagcat cccggcctat actcagggtt gcgttttcta aatttaattt ccggaaatta
     3001 aatttacgac tttcccagtc gggctgggaa aaagaggcta tctgattgtt ggacttatat
     3061 tccgtattgt aacgtttatt tacagtgctt tcctggtcct ttatggcagc agaatagtct
     3121 ttttgacata aaaaaggaat tggacagttg ttattgcctt ctttaaatat ggcatgttct
     3181 gtcgcatgtc cttgtaatgt taactgtgca ttccggacag agatatcgtt tttgatatcg
     3241 actgagccat caaagatcag attggcatta ttgttatgtt gctgggtagt attgataata
     3301 tgatttatgc ttatattacc tgatacattc ccatggtaca tatatgcatc agtattagta
     3361 agtgataatg ttgattgctt cacgtttgag ttcgttacgg ttgttcccgc atctgttgct
     3421 gcgattttct gaaatgactg gtcatagccg tttagatcca gtgttccgcc atttctggta
     3481 aaatagattt cgccattacc gctttcgctt aaagcgtctt tggcatttat ttttaccgta
     3541 cctttacctc cggccatgta aattttattg aaggcttttt cagcactaag gatgacagtc
     3601 ccattaccta ttttaaggtt atttccctgt gctattttta catccagagt accactacct
     3661 attttatgca gattatctcc ggcaacgccc ttaacattcc attcaacggt ggtattatta
     3721 tcaatatcta tcccggcacc tttataagac ttatcttttc cgtacacttt atatgtctgg
     3781 ttcttatcga atataagccc gccaatgcca agatcggtat cctgcttgag cgttaatacc
     3841 cctcctcctg agacagacag gtccttatct tttataatcg atgataactc ttgtttttta
     3901 ccatccagct gcatagtgtt tgtgaaattc ttttttaaat tgtttaccag aatattatta
     3961 aataacgagt agtttgtcca ggctatattg gtactactga taaaaccaac tgtactggta
     4021 acaccatgga aaacccattt ttgttgttct ttatcatata gataagaacc agaaccacta
     4081 tcgccaaaaa ttggtgagtt tttaaaaggg gttctgttgg tataaatgta actcttgttt
     4141 ttccagtcca gattaaaaag acttgcgctt aacaagtcgg gattatagtt ttgtccaaac
     4201 agatattgtt tgccgtttaa tatcgttgag gttgttccgg cacctgctct gaagccaatt
     4261 atttgttcct taccgttgta gtttacgcca tatcttttca tcgcatcttc gggagaaagt
     4321 gagaagtcgg cactctccgt aacccctgtt gtttccacga cgaatttatt cagacgatga
     4381 accgaaaagt cgccagatga tactctgtta cttgctttat atgctgtctg atcccatgac
     4441 tgtgttgtta ttgcatgatg ctgtgtaccg ttatgagtcg cagtaacact gtacgcacct
     4501 ccaattgatg ttgttgcacc tttgttggaa acagcagaaa aatcaggtac aggtagttct
     4561 ggaaaatgaa atatctttcc atctttcatc attaattgaa catttgttgc acccggtctg
     4621 aactctcctc tgttttgagc aaggtcaaga taatcgcggg tccatatatt atttatatcc
     4681 agcattgctg caaatgctgt ttcgccataa cttccatata ttgaggataa aataataccg
     4741 aggataactt tctttcttga tgtaccgaca ctaactttcc gggtcagttc agaaacagca
     4801 actaatccac ctgtaatatg actatatttc agtgaataaa ttttattcat acgtgaactc
     4861 cgatatttct gagttgtggt tatgggatag catttaatta caacggggaa ctatcagtca
     4921 tggtacgtaa ctgataaggg tataatgata atcactgtca gttatatttg tatgacagat
     4981 gtaacaaaat gaaaaaaaag ataagcacaa gtgattgatt ggttagatat tgatcacttg
     5041 gtgtttgtat tttgaatttt gcgatcaaaa tttgatcttt gttgtttccg gtcaatcctt
     5101 tacaggtttt ctgtattatc cgggggagtt aagtctgtcg atgtccgaca gacttttgat
     5161 gatggaaaca acatgcagcg ataatgtgca tgctgttttg caggtggaac tcagaaggtg
     5221 aactggaact gtgcgaccgc accgtaagtg ttgtccgtgc cgatcaggcc tgcaacatca
     5281 aggtgaacca catcaaacgg tgagatcccc accccggccg tgaatgcact gccgtcgtta
     5341 ccggccagat tctgacggta accggcgcgc agctgtgccc atttccaggc attaaattct
     5401 gtgccaatgg cggcaaactg acggttgctg tcggaggtga aaccgctggc cggggtcaga
     5461 tccacatcaa atgcagtggt gaacatcgcg ttgtgccagg agacaccggc cgtcacctgc
     5521 gggcggatcc tgaaggtttc cgtgataccg ttcacttctt ttgtctcaat actacgggag
     5581 atcaggttct gtgcgaccag tcccagcgtc cagttgtcat ccaggtccgt actggcaccg
     5641 atgtcggcgt tgatgccatt tttcgtgttg tggtagcggt cgcctttgaa ggcgctgctg
     5701 tcataatttc tgaccagcac gttgtagttg aacaggtcga cgcgctggaa tttggggggg
     5761 aagcccagag accatttctg cccggccgtt tccagttccg tagccagagc aatacccaca
     5821 tcagtgatca gtgccgtgcg ggcacaggcc tgtgatgtca ggcggctttt gtccacctcc
     5881 tgcccggtgc tgtttgccac ctcttccagg taagtgaggt cagcatcaga gactttaccg
     5941 tcgacactga cggtgccgtg tgatttaacc atcagtgcgg cagagaggcg atcaccggga
     6001 agggccgcca cagcagaaac gcccagctgt gcggcggcat gtgtgttacg gaagtcccgg
     6061 aggtgttctt tcaggcgtgc ggcagcctca ggaacacagt ggttactttc gatggcgcgg
     6121 tcgaaggcct tccactcatc actgatccgg tcggcgttgt cggtgatatt atccggattg
     6181 gagagctgcg cgccgacgga cggaagcagg atgctgggct catgcgcgac gcaaaatcca
     6241 cgatgtatat atcagtacta aaagcgcgac ggcggaagaa gctctgaaac taatcggtga
     6301 actgtacgcc atagagcacg atatacgcgg gttaccggtg tctgaacgcc tggcggtcag
     6361 gcaaatgcag agtagaccgc tcctgacttc cctgtataag ctgatgcagg agaaagaaca
     6421 cacgttatcg aagaaatgtc gtctgggaga tgcgttccgg tatatcagga agcactgggc
     6481 tgcgttgtgc aacttctgtg atgatggcct ggctgaggta gataataatg cagcagaaag
     6541 agcgcttcgt gcagtctgtc tcgggaagaa aactttttcg aggatcactc attccgtgtc
     6601 tgactgcttc agggtttaat tttgcttctg ctgactgaat gatgattggg cagcaagtgc
     6661 aatatcccac ataaatccag tcaactcacg agccaccgcc gtaatggcta cttgtgattt
     6721 tttccctttt gctgtcagag ttcggtaacg agaacacaat cgttgttgag ctttccatgc
     6781 aatatctttg gaatattgtg ttacagagtc cggtcggtaa tatataagcc atgctccaac
     6841 ttttgcaggt gtacgataag accaggcggc ttcgtaaagc agacgtctca gttcgctgtt
     6901 tccaactttt gttattcctc tgggacgaat actgtttcca ctggaatatt cacctggcac
     6961 cag
//
LOCUS       DAJTUI010000156         6927 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTUI010000156 DAJTUI010000000
VERSION     DAJTUI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.156"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..797
                     /locus_tag="OO464_003777"
     CDS             <1..797
                     /locus_tag="OO464_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6228754.1"
                     /translation="IGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAAS
                     GGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVD
                     YLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKQGP
                     YEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVV
                     MRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1167..1991
                     /gene="gadX"
                     /locus_tag="OO464_003778"
     CDS             1167..1991
                     /gene="gadX"
                     /locus_tag="OO464_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6228755.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            2359..3087
                     /gene="gadW"
                     /locus_tag="OO464_003779"
     CDS             2359..3087
                     /gene="gadW"
                     /locus_tag="OO464_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6228756.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(3450..6563)
                     /gene="mdtF"
                     /locus_tag="OO464_003780"
                     /pseudo
     CDS             complement(3450..6563)
                     /gene="mdtF"
                     /locus_tag="OO464_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(6588..6845)
                     /locus_tag="OO464_003781"
                     /pseudo
     CDS             complement(6588..6845)
                     /locus_tag="OO464_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1782 a         1669 c         1670 g         1806 t
ORIGIN      
        1 ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag ttcccgcaac
       61 cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc gacatgcaca
      121 tcgacgccgc cagcggtggc tttctggcac cgttcgtcgc cccggatatc gtctgggact
      181 tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt ctggctccgc
      241 tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa ctggtgttca
      301 acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc cgcccggcgg
      361 gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc tataccaaag
      421 tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc aaacaggggc
      481 cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt tgcttcaaac
      541 tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt ctgcgtctgc
      601 gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc gtggtgatgc
      661 gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg gaagactaca
      721 aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc cagcagaaca
      781 gctttaaaca cacctgataa cataacgttg taaaaaccga atgcccagcc tttaaaaaaa
      841 cagctgggca ttcggttgct tattaatcgc aataatatat tggctgtcta ttcatcgtgt
      901 tgataagata tatgttatat gaatgtttat taaatgtagc gattaccttt aatgtcacga
      961 aaagggatta tttgcttgct attaatttca ctgcgttctt aaacacgttt ttgtattata
     1021 aatccagtca tcctgcccgt agaacatcac acattatcat cctgttctcc cgcttcgttt
     1081 aaatttattt atcaatcaat ttgacttaag agggcggcgt gctacattaa ttaacagtaa
     1141 tatgtttatg taatattaag tcaactatgc aatcattaca tgggaattgt ctaattgcgt
     1201 acgcaagaca taaatatatt ctcaccatgg ttaatggtga atatcgctat tttaatggcg
     1261 gtgacctggt ttttgcggat gcaagccaaa ttcgagtaga taagtgtgtt gaaaattttg
     1321 tattagtatc aagggatacg ctttcattat ttctgccgat gctcaaggag gaggcattaa
     1381 atcttcatgc acataaaaaa atttcttcat tactcgttca tcactgtagc agagatattc
     1441 ctgtttttca ggaagttgcg caactatcgc agaataagaa tcttcgctat gcagaaatgc
     1501 tacgtaaaag agcattaatc tttgcgttgt tatctgtttt tcttgaggat gagcacttta
     1561 taccgctgct tttgaacgtt ttgcaaccga acatgcgaac acgagtttgt acggttatca
     1621 ataataatat cgcccatgag tggacactag cccgaatcgc cagcgagctg ttgatgagtc
     1681 caagcctgtt aaagaaaaaa ttgcgcgaag aagagacatc atattcacag ttgcttactg
     1741 agtgtagaat gcaacgtgct ttgcaactta ttgttataca tggcttttca attaagcgag
     1801 tcgcagtgtc ctgtggatat cacagcgtgt cgtatttcat ttacgtcttt cgaaattatt
     1861 atgggatgac gcccacagag tatcaggagc gatcggcgca gggattgccg aaccgtgact
     1921 cggcggcaag tattgttgcg caagggaatt tttacggcac taaccgttct gcggaaggaa
     1981 taagattata gagttttact cagacataaa aaaaacccgg cataggggac cgggaagagg
     2041 atagtctgcc gtctccagac taataaaccg ttataacact ccctgttggc acgggaaact
     2101 ttgtgctctc agtaagttaa atataacttt tacaggaaat aagatcagcc atttttttat
     2161 aaacataagc tatacgctgt gcgaaaatat attcgtgctg catttactta ttatcaatta
     2221 actgttatgc aaaactactt tgtggataaa ttttggtcct accaaatctg gcagtttttg
     2281 cgctaagaaa cagtctgtca tcatttcatt agtatactga aattgaaata atcgcagtat
     2341 gaaatataag ggataatcat gactcatgtc tgctcggtga tcctcattcg tcgttcattc
     2401 gatatttatc atgaacagca aaaaatatcg ctgcataacg agagtatcct gctgctggag
     2461 aaaaatttgg cagacgattt tgcgttttgt tcaccggata cgcgacgact ggatatcgat
     2521 gagctgacag tttgccatta cttacaaaat attcgtcagc taccacgcaa tttagggtta
     2581 catagcaaag accgtctgtt aattaaccag tcacccccca tgccgctggt gacggcgatt
     2641 tttgatagct tcaatgaatc cggggtaaat tcaccgatac tgagcaatat gctctacctt
     2701 tcctgtttat cgatgttttc tcataagaaa gaactgatcc ccttactttt caatagcatc
     2761 agcactgttt caggaaaagt tgaacgcctt attagctttg atatcgccaa acgttggtat
     2821 ctgcgcgata tcgcggaaag aatgtatacc agcgagagtc tcatcaaaaa aaagttgcag
     2881 gatgaaaata cctgtttcag taaaatatta ctcgcttcca ggatgtcgat ggccagacga
     2941 ttactcgagt tacgtcaaat tcctctgcat actattgcgg aaaaatgtgg ctatagcagt
     3001 acgtcgtact ttataaacac atttcgacaa tattatggtg taacgccaca tcagtttgcg
     3061 caacattcgc caggtacctt ttcctgacat attttgcatt tgaatattgg tcaggatctc
     3121 acacctgctt catgtgaaac tcttccctga tgatttctgc cgggctaccg gctagttctc
     3181 tttcgcagtt tgccaggcgg tataggtatt tttaggagga aaagactcgg catgtttggg
     3241 attattaagc tgacaattca tacgattacg ggaatgtggg tcagtattgt gttgttcaaa
     3301 ttgatgacga atggctggag tggtttctac tttcagtgct gtgtactaag cctggtattc
     3361 ctgacggtta gctggttgtt gagtggtgaa tggttagcag gaaagagtaa ggctgaacct
     3421 tcacgttcaa ccttactctc atttacacgt tacgcttttt taaagcgggc aaagagatgt
     3481 tccactacaa cgaaaaagac cggaacgaag taaattgcca gcactgttgc ggcaaacatc
     3541 ccgcccatca cgccggtacc taccgcgttt tgcgcgccag aaccggcacc atgactgata
     3601 accagcggca gcacgccgag aataaaggcc agagaggtca tcaggattgg gcgtaaacgc
     3661 atccgcgccg cttcgatgat tgcctctatc ggcgttttcc cttctttctg catcatctca
     3721 acggcaaatt cgacaatcag gatggcgttc ttggcggaaa gcccgatggt ggtcagcaaa
     3781 ccaacctgga agtagacgtc attacttaag ccgcgcagat cggtggccag taatgcgcca
     3841 acgacgccta acggaacaac caacatcacc gagaacggaa ttgaccagct ctcatagagt
     3901 gcggcgaggg cgaggaacac cacgaccagt gaaatggcat acagtgcagg agcctggttt
     3961 gaggataacg cttcctgata cgacagtccg gtccatgagt agccgacgcc tgccggaagt
     4021 ttagcgacca ggtctgccat aaatttcata gcgtcaccgg tacttttccc ggccgccgct
     4081 tcacctaaaa tctccattga cgggatgccg ttgtagcgtt ccaatcgcgg tgaaccatag
     4141 gtccattcgg tagacgagta ggcagaaagc ggtgccatcg tgccagaggc gttgcgtaca
     4201 taccattggt tgatgttatc cggcaacata cggaacggcg tgcctgcctg gacatacact
     4261 tttttcaccc gcccctggtt gaggaagtcg ttcacgtagc tgctgccgaa ggcggtggaa
     4321 attgtctggt tgatatcaga cagcgccaca cccattgcct cagctttcgc ggcgttgacg
     4381 ttcactttga acatcggcgt atcttccagg ccgttcgggc gtaccccggt gacctggtca
     4441 ggcgattgcg ctgccagtga taacagctcg tttcgtgcct aggttagttt ttcgtgcccc
     4501 aggttaccgt tgtccagcag ttccatatca aaacctgacg cggtacccag ttcagccacc
     4561 gcgggtaagt tgaacgggaa gacgacggct ttattgatac tgcttaacgc aatcattgcc
     4621 cgctgaatga tcgcggtaac cgagttttcc tcaccgacac gttcagacca cggcttgaga
     4681 ctgataaacg ccaggccgtt gttttgcccc tggccgctga agccaaagcc gccaacggta
     4741 aacaccgact gaacattatc tttctctttt gtaagataat aatccgtcac ctgttgcagt
     4801 actttcgtgg tgttaaccat ggtggcaccg gaaggtaact gcgcggtggt cataaatacc
     4861 ccctgatcct cttctggtaa gaaagaggtc ggtgtacgca ggaacagcac cgccatcccg
     4921 gcgcaaatca gcaggtagac caccatgtag cgaccggtac aacgcaacag cgagcgggtg
     4981 ctatcggtat agtgctgagt tgatttttca aacagcgtgt tgaagcgtgc gaacagggcg
     5041 ttaggtttgt gaccgccttc cggcgtggct ttcagaatgg tggcgcacag ggcaggggtc
     5101 aggctcattg ccacaaatac tgaaagccgc atggaggaga tcagcgtgat ggagaactgg
     5161 cggtagatct ccccggttgc accgctcata aaggccatcg gcataaacac tgcagaaaga
     5221 acaacggcaa tcccgaccag cgcacgttgg atctgcccca tcgatttatg cgtcgcttcc
     5281 ttcggcggta gcttatcttc cgcaatgaca cgctcgacgt tctccaccac cacgatggcg
     5341 tcatccacca gtaaccctat cgccagcacc atcccgaaca tagtcaacgt gttgatggtg
     5401 aaaccgaccg ccgacaagat cgcaaacgtc ccgagaataa ccaccggtac ggcaattgtc
     5461 gggatgatgg tggcacggaa attctgcaaa aacagataaa tgaccaggaa gacgaggatg
     5521 atagcctcaa ccagtgtttt gaaaacttcc tgaatagaaa tttcgataaa cggcgtggtg
     5581 tcgtaaggat aaaccgtctt cagacttgcg gggaaatagg ctgataagcg gttcagctcc
     5641 tctttgactg cccgcgaggt atccagggcg tttgctccgg cagccagttt gatggcgatc
     5701 ccggcaacag gtttgccgtt atagcgcgcc acggtggaat aatcttccgc cccaagttcg
     5761 acgcgggcga catcacgcag cagcacttgc gaaccatctt gctgaacttt caacaggatt
     5821 ttgccaaatt cttccggcgt ttgcagacgc gtctgcacaa tgatcgaggc gtttagttgc
     5881 tggtctgccg cctgtggcat gccacccagt tgaccaccag aaatctggtt gttttgcacc
     5941 ttaatctggg aaataacatc ggaaggtacc aggttatatt tattgagttt ttgcgggtcc
     6001 agccagatac gcatggcgta ctcggaacca aagagctgta cgctaccaac gcccgcggta
     6061 cggcttagcg ggtctttgat attagacgct acatagtccg cgatatcgta ctggttgagg
     6121 ctgccgttat cagaaataaa cgccgctacc atcaggatat tactgctcga cttatcgacg
     6181 ctaatcccct gttgctgcac cgcttcaggt aatgaaggca tagcgagttg cagtttattt
     6241 tgcacctgaa cctgtgcgat atcaggagat gccccagtct cgaaggtcag agtgatagag
     6301 gcattacccg ccgcatcact ggttgaagac atgtacatca ggccatcaag cccattcata
     6361 ttttgctcaa tcacctgagt gaccgagtct tctaccgttt gcgcatcggc acccggatag
     6421 gtagcgctga cggtaatggt cggtggcgca atctgcggat actgcgcaac cggtaagttc
     6481 atgatcgcca gaccacctgc aagcatcata ataatggcaa gtacccaggc aaaaaccggg
     6541 cgatcaataa aatagttagc catgaaagtc cccttaagcc tgcaacgtta ttgtttcgat
     6601 tcggtgctgg cgttttcctg gctggaggaa attgctcgtg ctttgatacc cggacgaatg
     6661 cgttgcaaac cggaaacgat cacccgatcg ccagcctgca agccagaggt gacaacccac
     6721 tggtcgccga tggctttgct ggcttcaatt tcgcgtagct gcacgacatc gtctttatcc
     6781 agaatgagcg ccgttgcttt accctgggcg ttgtgggtga cgccttcctg cggcaccagt
     6841 aatataaagc gcgcaaaatt attcaagata tttttgaaaa tcattcaaga taggctatcg
     6901 cgtgtgatag caaaaacatt caagata
//
LOCUS       DAJTUI010000157         6921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTUI010000157 DAJTUI010000000
VERSION     DAJTUI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.157"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(44..946)
                     /locus_tag="OO464_003782"
     CDS             complement(44..946)
                     /locus_tag="OO464_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR6228757.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1025..1978)
                     /gene="lsrR"
                     /locus_tag="OO464_003783"
     CDS             complement(1025..1978)
                     /gene="lsrR"
                     /locus_tag="OO464_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR6228758.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2227..3762
                     /gene="lsrA"
                     /locus_tag="OO464_003784"
     CDS             2227..3762
                     /gene="lsrA"
                     /locus_tag="OO464_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR6228759.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            3756..4784
                     /gene="lsrC"
                     /locus_tag="OO464_003785"
     CDS             3756..4784
                     /gene="lsrC"
                     /locus_tag="OO464_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR6228760.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            4784..5776
                     /gene="lsrD"
                     /locus_tag="OO464_003786"
     CDS             4784..5776
                     /gene="lsrD"
                     /locus_tag="OO464_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR6228761.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            5788..6811
                     /gene="lsrB"
                     /locus_tag="OO464_003787"
                     /pseudo
     CDS             5788..6811
                     /gene="lsrB"
                     /locus_tag="OO464_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
BASE COUNT         1597 a         1756 c         1817 g         1751 t
ORIGIN      
        1 ctacagtctc accacagcga tcactgcttt caaaggatgg gactcatgac ggcatacgga
       61 acggagtaat cggcaactga tgccgggcct cgctgagcgt gcactgctta ttatccgtga
      121 acgtgccgcc aagaaccgcg gtttgtgccg gacgcacaac gcctaaccca aggcaaccaa
      181 gctgcacgtc gcctcctcca acgaccacca gagtgcccgc cttcagaccg cagagttccg
      241 ccgcttgtga acttaccacg cccagcaatg tgccggtttc tttgacagga gaaagaatat
      301 cggcacgcag gccagccata tccagcaatg caggtttcca ggcacgggtg gttagatcaa
      361 gaagtcccgt ggtgccagcg ttagagggat ccaccgccag ttcgccgctg agcatatagg
      421 ccagccagtc gctgatcatg gtgatggttg atgcctgacg gtaaatatcg gaacgatggt
      481 gcgccagcca aagtaatctg gggatggcac ttaaagccag tgtttgtccg gtcgctcgat
      541 aaacttcgtt ttcaaaggta ttgttgtgca gttctttaag ttcgctaact tcgcgtgccg
      601 ctctggcatc cacattggcg caggcccaga tcggtgttcc ttcattgtta tataaaacaa
      661 tgccttcacg catagaacat gccgaaacgg cagcgatata ctccgggtct atgccggcgt
      721 tgtgcagcgc ctggcgcata cactcacacg ccagttgcca gtttttgttg agatcaaatt
      781 ccatagaacc aggaacgtcc ggtactgcca gatgccgcca ctccgcctgt cccactgcta
      841 tttgattgcc ttccaggtcg aatatcacag cccgaatact tccggtgcct gcatccagcg
      901 ccatcaggta gtactttgat tctgaagggg taaagagtcg agccattaga ttatcctcgg
      961 cttatgcgtt ctgtctgttc ctctatacgt tctccatcat tcccggtaat aaggtcatgc
     1021 aaatttaact acgtaaaatc gccgctgctg tgtcctgatc ggtaaccagt gcgttgatat
     1081 aaccgccttt cattgcagcg gcaattgctt cagctttatt ttctccccct gccacgccaa
     1141 cccggacggg tatggtcttc agcgcgctta aaggtaagcc aatcagttcg ttatgtattt
     1201 tgatatccgt gacaacgtca ccttttgcat caaaaaagta gcctaaaatg tcgccaaccg
     1261 ccccttttcg gccaatcatt aactgttcgc cctggctgat ataaccggag cgaatgattg
     1321 tcgcgtcgtc ttgttgactc acggctccaa tgccgacaat cgccacatcc gctgcttgcg
     1381 cggctagcag aacatctttg acgcaatttt cattttttag cgtacgggca atgtcagcgg
     1441 aggatgcccg caacggagcc ggaataatat tcacactgca cgccgcgtta agctgcccga
     1501 tacccgtcat ataagaaccg acgccaccgg agagcgtgac caggcgaatt tgctgtgacg
     1561 aaataaaacc acttaagcgt tgcagcgtat tcatggttgc ctcgccaaaa ccaatcgcca
     1621 gcatctgttg tggttgaagt aaactcatca acatatgcgc cgcgcctatc cctagtcgcc
     1681 cgccgacatc agcatccgca agcccaggga tcacccggac atgttgcagc gaaaactgac
     1741 gacgtaattg agtttcatat tccagacagc cttcaaagcg agaattaatc tgtacgcgaa
     1801 taatgccgga ctgatgccct ttctccagca agcgcgacac tttcaaacgg gtcaggccga
     1861 gacgatcgct gatctcgctc tgggtcagcc cgtcgtgata gtaaaaccac gcgatccgcg
     1921 cgacctgttc ttcttcacac attccctgtt ctgaaattgc cgaatcgttg attgtcataa
     1981 ttcattcttc actttgaaca tatttaaatc tttaatgcaa ttgttcagtt cttgcgcatt
     2041 tatatctgtg atggcaacca cagtttgact ctacgagcat gaacaaacgc aaccgtgaaa
     2101 atcaaaatag cataaattgt gatctattcg tcggaaatat gtgcaatgtc cacctaaggt
     2161 tatgaacaaa ttaaaagtag aaatacattt gttcaaaact cacctgcaaa actgaacggg
     2221 ggaaatatgc aaacgagtga tacccgcgcg ttaccgctac tttgcgcccg ctcggtttat
     2281 aaacagtatt caggggtcaa tgtcctgaaa ggcatcgatt ttacgttgca tcagggggag
     2341 gtccacgccc tgctcggcgg caatggtgcc ggtaaatcga cgttaatgaa gattattgcc
     2401 ggtattaccc ctgctgatag cggtacgctg gagattgggg gcaacaacta cgccagatta
     2461 acgccagttc atgctcatca gctgggcatt tatctcgttc cccaggaacc gctgcttttc
     2521 ccaagcctgt cgataaaaga aaacatcctg tttgggctgg caaaaaaaca gctctccatg
     2581 cagaaaatga agaacttgct ggcggcgctg ggctgccagt ttgatctgca tagtctggca
     2641 ggatcgctgg atgtcgccga tcgccaaatg gtggaaatcc tccgcgggct gatgcgcgac
     2701 tcgcggattc tgatcctcga tgaacctacc gcctcgctta cccctgcgga aaccgaacgc
     2761 ttgtttagtc gcttgcaaga gctgcttgct actggcgtgg gtattgtttt tatctcgcat
     2821 aagctgccgg aaattcgcca gattgccgat cgaattagcg tgatgcgcga cggaaccatc
     2881 gccttaagcg gcaaaaccag cgaactgtct accgacgaca ttattcaggc catcacccca
     2941 gcggtacggg aaaaatcgct ctctgccagc caaaaattat ggctggagtt acctggtaac
     3001 cgcccacaac atgccgtcgg aacgccggtg ctgacactgg aaaatctgac tggcgaaggc
     3061 ttcaggaatg tcagcctgac gctcaatgcc ggagaaattc tgggcctggc tgggctggtg
     3121 ggagccggac gcacagaact ggccgagacg ctctatggtc tgcgtacttt gcgtggcgga
     3181 cgcattatgc tgaatggtaa agagatcaat aaattatcca ctggagaacg tttactgcgc
     3241 ggtctggttt atctgccgga agatcgccag tcatccggac tgaatctcga tgcttcgctg
     3301 gcctggaacg tctgcgccct tactcataac cttcgtggat tctgggcgaa aaccgcgaaa
     3361 gataatgcca ccctggaacg ttatcgtcgg gcgctgaata ttaaatttaa ccaaccggaa
     3421 caagctgcac ggacattatc cggtagcaac cagcaaaaaa tcctcattgc caaatgcctg
     3481 gaagcttcgc cgcaagcatt gattgtcgat gagccgacgc gcggcgtgga tgtctcggcc
     3541 cgtaatgata tctaccagct gttgcgcagc atcgccgcac agaatgtggc tgtgctgttt
     3601 atctcctctg atatggaaga gatcgaactg atggcagatc gtgtgtatgt gatgcatcag
     3661 ggcgaaatta cccactctgc actgaccggg cgcgatatta atgtcgagac tattatgcgc
     3721 gttgccttcg gcgatagtca gcgtcaggag gcatcatgct gaagtttatt cagaacaacc
     3781 gtgaaatcac ggcattgctg gcggtggtgc tgctgtttgt attacccggt tttctcgacc
     3841 gccagtattt aagtgtgcaa acgctgacca tggtttatag cagcgcgcaa atcctgatcc
     3901 tgctggcaat gggcgcgacg ctggtaatgc ttacgcgcaa tattgatgtt tcagtgggtt
     3961 cgattaccgg aatgtgcgcg gtgctgttgg ggatgttact gaacgcagga tattcactac
     4021 ctgttgcctg tgtcgcgact ttactgcttg gtttgctcgc gggatttttc aacggtgccc
     4081 tggtcgcgtg gctaaagatc cctgccattg ttgccaccct tggcacgtta gggttgtaca
     4141 gaggcatcat gttgctgtgg actggcggta aatggattga agggttaccc gccgaactga
     4201 aacagctctc cgccccgctg ctgcttggca tttcagcaat tggttggttg acgataattc
     4261 tggtggcatt tatggcctgg ctgctggcga agacggcgtt tggacgcagt ttttatgcca
     4321 cgggcgataa tttacagggc gctcgtcaac tgggcgttcg tacggaagcc attcgcattg
     4381 tggcattttc gttgaacggc tgcatggcgg cactggcggg aattgtgttt gcttcgcaga
     4441 ttggttttat cctcaaccag accggtaccg ggctggagat gaaagcaatt gcagcctgcg
     4501 tgctgggcgg cattagtttg ctcggtggtt ccggtgcgat catcggtgcg gtactcggcg
     4561 catggttcct gacgcagatc gatagcgtac tggtgctgtt gcgcattccg gcatggtgga
     4621 atgattttat cgcgggtctg gttctgctgg cggtgctggt gtttgatgga cgcctgcgtt
     4681 gtgcgctgga acgtaatcta cggcggcaaa aatatgcccg ctttatgacg ccaccgccat
     4741 ccgttaaacc cgcttcgtca ggtaaaaaac gggaggccgc ataatgcgta ttcgctacgg
     4801 ttgggaactg gctcttgccg cactgctcgt tattgagatt gtcgcatttg gtgcaattaa
     4861 cccgcgaatg ttagatctca atatgttgct gttcagcacc agtgacttta tctgcattgg
     4921 cattgtcgct ctaccgctga cgatggtgat tgtcagtggc gggatcgata tttcgtttgg
     4981 ttcgaccatc ggcctctgcg ccattgcatt gggcgtactg tttcaaagtg gtgtgccgat
     5041 gccgctggcg atactcctga ccttactgct cggcgcattg tgcgggctga tcaacgccgg
     5101 attaattatc tataccaaag ttaacccgct ggtgattacg cttggcacgc tgtatctgtt
     5161 tgccggaagc gctctgctgc tttccggtat ggccggagcg acggggtacg aaggtattgg
     5221 tggattcccg atggcgttta cagatttcgc taacctggat gtgctgggac tccccgttcc
     5281 gctgattatc ttcctgatat gtctcctcgt tttctggctc tggctgcata aaacccatgc
     5341 cggacgtaat gtgtttttga ttgggcaaag cccgcgcgtg gcgctttata gcgcgattcc
     5401 agttaaccgc accttatgtg cgctctatgc catgacgggg ctggcgtctg cggtcgccgc
     5461 tgtgctgctg gtatcgtatt ttggttcagc acgttccgat ctcggtgcgt cgtttctgat
     5521 gcccgccatc accgccgtgg tgcttggcgg ggccaatatt tatggtggtt ccggttccat
     5581 tatcggcacc gccattgcgg ttttattagt gggatatttg cagcaaggtt tgcaaatggc
     5641 aggagtgcca aatcaggtgt ccagcgccct ttccggtgcg ctgcttatcg tcgttgtcgt
     5701 aggtcgttcc gttagcctgc atcgccagca aattaaagag tggctggcgc gtcgggccaa
     5761 taacccattg ccataaagga tatcttcatg acacttcatc gctttaagaa aatcgcctta
     5821 cttagcgctc ttggcattgc cgcaatctct atgaatgtgc aggccgcaga gcgtattgca
     5881 tttattccca aactggttgg cgtgggattt tttaccagcg gtggcaacgg cgcacaacaa
     5941 gcgggtaaag agctgagcgt tgatgtgacc tacgacgggc cgacagaacc cagtgtttct
     6001 ggtcaggtac agttgattaa taacttcgtc aatcaaggtt ataacgccat tattcgtttc
     6061 tgcggtttcg cctgatggct tgtgtccggc actgaaacgc gccatgcaac gtggtgtgag
     6121 agtgctgacc tgggactctg atactaaacc ggagtgccgc tcttactaca ttaatcaggg
     6181 aacgcccgcc cagttgggag gtatgttggt ggatatggcg gcgcgtcagg tgaataaaga
     6241 caaagccaaa gtcgcgtttt tctactcaag ccccaccgtt acggaccaaa accagtgggt
     6301 gaaagaagcg aaagcgaaaa tcgccaaaga gcatccaggc tgggaaattg tcactacgca
     6361 gtttggctat aacgatgcca ctaaatcgtt acaaaccgca gaaggaatat taaaagcgta
     6421 tagcgatctc gacgccatta tcgcccccga tgccaacgcc ctgcccgctg ccgcacaagc
     6481 cgcagaaaac ctgaaaaatg acaaagtagc gattgtcgga ttcagtacgc caaatgtgat
     6541 gcgcccgtat gtagagcgcg gcacggtgaa agaatttgga ctgtgggatg tggttcagca
     6601 aggcaaaatt tcagtgtatg tcgcggatgt attattgaaa aaaggatcaa tgaaaacggg
     6661 cgacaagctg gatatccagg gcgtaggtca ggttgaagtc tcgccaaaca gcgttcaggg
     6721 ctatgactac gaagcggatg gtaatggcat cgtactgtta ccggagcgcg tgatattcaa
     6781 caaagagaat atcggcaaat acgatttctg atgtgcatta cttaaccgga gtaagttatg
     6841 gcagatttag acgatattaa agatggtaaa gattttcgta ccgatcaacc gcaacaaaat
     6901 atccctttta ccctgaaagg t
//
LOCUS       DAJTUI010000158         6795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTUI010000158 DAJTUI010000000
VERSION     DAJTUI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.158"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            240..2672
                     /locus_tag="OO464_003788"
     CDS             240..2672
                     /locus_tag="OO464_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR6228762.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="OO464_003789"
     CDS             2739..4208
                     /locus_tag="OO464_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6228763.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..5869
                     /locus_tag="OO464_003790"
     CDS             4208..5869
                     /locus_tag="OO464_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR6228764.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6090..6431
                     /gene="symE"
                     /locus_tag="OO464_003791"
     CDS             6090..6431
                     /gene="symE"
                     /locus_tag="OO464_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR6228765.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6478..>6795)
                     /locus_tag="OO464_003792"
     CDS             complement(6478..>6795)
                     /locus_tag="OO464_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228766.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         2003 a         1612 c         1554 g         1626 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag
     5701 tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa
     5761 gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa
     5821 aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc
     5881 tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt
     5941 cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga
     6001 tgatgtatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc
     6061 acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt
     6121 tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt
     6181 acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg
     6241 gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg
     6301 cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc
     6361 tgtcggcgcg taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga
     6421 aagtcgcctg atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta
     6481 taccaacgcg ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg
     6541 aagttgtgcg aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt
     6601 tgtcacatct ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga
     6661 caatcgcagt cggctttttt gcggcacaac atcaacaaaa ttcgtttccg ctttaaacgc
     6721 aatatatagc ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa cttcatcgcg
     6781 aagatgatcg aataa
//
LOCUS       DAJTUI010000159         6710 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTUI010000159 DAJTUI010000000
VERSION     DAJTUI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.159"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..795
                     /locus_tag="OO464_003793"
     CDS             <1..795
                     /locus_tag="OO464_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6228767.1"
                     /translation="RYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVL
                     SSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVT
                     LTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSRKSVLFWILT
                     ALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIA
                     IDMDKLKQINDTLGHQEGDLAITLHDAYKASDERLYVNKQNKNSRS"
     gene            838..1341
                     /gene="yeaK"
                     /locus_tag="OO464_003794"
     CDS             838..1341
                     /gene="yeaK"
                     /locus_tag="OO464_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR6228768.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1342..1446)
                     /locus_tag="OO464_003795"
     CDS             complement(1342..1446)
                     /locus_tag="OO464_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228769.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1616..2062
                     /locus_tag="OO464_003796"
     CDS             1616..2062
                     /locus_tag="OO464_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR6228770.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2019..2837)
                     /gene="nimR"
                     /locus_tag="OO464_003797"
                     /pseudo
     CDS             complement(2019..2837)
                     /gene="nimR"
                     /locus_tag="OO464_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            2934..4114
                     /gene="nimT"
                     /locus_tag="OO464_003798"
                     /pseudo
     CDS             2934..4114
                     /gene="nimT"
                     /locus_tag="OO464_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4169..4516
                     /locus_tag="OO464_003799"
     CDS             4169..4516
                     /locus_tag="OO464_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR6228771.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4538..4792)
                     /gene="yoaF"
                     /locus_tag="OO464_003800"
     CDS             complement(4538..4792)
                     /gene="yoaF"
                     /locus_tag="OO464_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR6228772.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            4975..6000
                     /gene="dgcP"
                     /locus_tag="OO464_003801"
     CDS             4975..6000
                     /gene="dgcP"
                     /locus_tag="OO464_003801"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR6228773.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6033..6131
                     /locus_tag="OO464_003802"
     CDS             6033..6131
                     /locus_tag="OO464_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR6228774.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6134..6208
                     /gene="yoaJ"
                     /locus_tag="OO464_003803"
     CDS             6134..6208
                     /gene="yoaJ"
                     /locus_tag="OO464_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR6228775.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6369..6560)
                     /locus_tag="OO464_003804"
                     /pseudo
     CDS             complement(6369..6560)
                     /locus_tag="OO464_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004995861.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1612 a         1627 c         1638 g         1833 t
ORIGIN      
        1 cgctatgtct atttttatga gccggttaat gttgaatact ttgcgatgaa taactggtcc
       61 ttcctgcagt caggaagtat tggcatcgat cgcaaagata ttgaaaaggt atttaccggg
      121 cgtaccgtat tgtcgagcat ttaccaggat cagcgtacta aacagaacgt gatgagtttg
      181 ctgacgccgg tatatgtcgc agggcagcta aaagggattg tgctgctgga tattaacaaa
      241 aacaatttgc ggaatatctt ttacactcat gaccgccctc tcctctggcg ttttctcaat
      301 gtcacgctaa ccgataccga ttcggggcgc gacattatca tcaaccagag cgaagataat
      361 ctgttccagt atgtcagtta cgtccatgac ttacctggcg gcattcgtgt ctcgttatcc
      421 attgatattc tttactttat cacgtcttcg aggaaaagcg ttctgttctg gattttgacg
      481 gcgttaattt tgctgaatat ggtccggatg cacttccgtt tataccaaaa tgtgtcgcga
      541 gaaaatatta gtgatgcgat gactggactg tataatcgca aaattttaac ccctgaactg
      601 gagcagcggt tgcagaaact ggtgcaatcc ggttcttcgg tgatgtttat tgcaattgac
      661 atggacaagt taaagcaaat aaatgacacc ctcggtcatc aggaggggga tttagcgatt
      721 acgttacatg atgcgtataa agcttccgat gagcgtttat atgtcaataa gcagaacaaa
      781 aacagccgtt catgataacc ttctgtggtt gtttgcttgt aatctcagga gcgtgaaatg
      841 actgaaatgg ctaaaggaag cgtgacgcat cagcagttaa tcgctttatt atcacaagaa
      901 ggtgctaact tccgcgtcgt tacccatgaa gcggtaggta aatgtgaagc ggtgtcagaa
      961 attcgtggta ccgctctagg ccagggtgct aaggcactgg tatgtaaagt caaaggcaat
     1021 ggcgtaaatc aacatgttct ggcgatcctc gcagccgatc aacaggccga tctgagccaa
     1081 ctcgccagcc atataggcgg attacgcgct tcgttggcca gcccggcgga agttgatgaa
     1141 ctcacaggct gtgtcttcgg cgcgatcccc cctttcagct tccatccaaa actcaaactg
     1201 gttgccgacc ctctactctt cgaacgattt gatgaaatcg ccttcaatgc agggatgctg
     1261 gataaatccg ttattttgaa aaccgcggat tatttgcgca ttgcgcaacc agaactggtc
     1321 aatttccgcc gcactgcgta gctagcctat ccgttcaata agcagaacgg acaaaaccag
     1381 cacaaccgca atagcaaaaa acgatgatgt gataatcagt gtctcgacga acatttgatc
     1441 gttcatagca tgcccccggg agatagctgt tttcacgtta ttgttagcgt gcacaaatgg
     1501 cagtttgatg acagttcgcc tgatttttta tgcaaaaaac gtaaagattt tctacttcct
     1561 tcctgcagca agcataaagt aagcaggctt attatttttt ggcaaggaaa ccacgatgtt
     1621 tgatgtcact ttgctgatcc tgctcgggtt agctgcgctg ggctttatca gtcataacac
     1681 cactgtcgcc gtttcaattc tggtgttgat cattgtccgc gtcacaccgt taagcacctt
     1741 tttcccgtgg attgaaaaac aggggctaag tatcgggata atcattctga cgattggcgt
     1801 tatggccccc atcgccagcg ggacgctacc gccctcaacc ttgattcact cgtttctgaa
     1861 ctggaaatcg ctggtggcaa ttgcagtagg cgttattgtc tcctggctgg gtgggcgcgg
     1921 cgtgacgtta atgggcagcc agccgcaact ggttgctggg ctgctggtgg gcactgtttt
     1981 aggcgtagcg ctgtttcgcg gcgtaccggt tggcccgctt attgccgcgg gtctggtttc
     2041 gctgattgtg gggaaacagt agttaatccc gcaatatatc ggcctggcgt ttgccccaag
     2101 cctttcttaa acatagtaat aaaggccgtt gttgaatcat accccagcgt atgagcaact
     2161 ttctgtacag tatcgccttt cactaatccc tgaagcgcca taatcagctg taactgctga
     2221 cgccactggc gaaaactcaa ccccgtctct ttcactatca ggcgggcaag attgcgttca
     2281 ctcatcgcaa aaaagccagc ccattgcccc aatgcccccc actcgacggg ccctttcgcc
     2341 atcatctcca ccatcgtgcg gattttagga tgagaagaaa cgggcaggtg cagctgttgt
     2401 tgtggctgct gagggagttc atcaaacagg acctgaatta atctgcgggt catcggctca
     2461 gcacgctgag tcgtcgtcct gtttgccagg gttaaaatca attcccggca taagggcgat
     2521 attttcaatg tgcaacatgg tcggcattgt cactgcgctg ggttcgataa acagaaaaca
     2581 gagttcagcg ttagctgtca cttgattgct atgcttcacc ccgcccggta tccaaaccgc
     2641 atattgtggc ggcaccatcc acaaagcatt ttccaccgta caggtaattg caccatgtaa
     2701 tgccagtatc agctgtcctt tgcggtgctg atgtacagga ctggttaact catccgttcc
     2761 ggcgtgaata caaaatgcga ccgcggcttc atggtgtcgg tgggttcata gccattgaga
     2821 ttcaacctgt gcatcatttt gtccgaactt agcgataatt tgtcatttta gcttgattca
     2881 acataacaat aaaaacggta aggtacagcc tcgtttgtaa caatgagaag catatgacct
     2941 gttcaacttc attaagcggc aaaaacagga ttgtccttat cgctggcatt ctgatgattg
     3001 ccacaacatt acgcgtcacc tttaccggcg cagcgccgtt actggatacg attcgttccg
     3061 cttactcgct gacgacagcg caaaccggct tattaaccac cctgccatta ttggcctttg
     3121 cgctaatctc acctctggct gccccggtgg cgcgacgttt tggtatggaa cgtagcctgt
     3181 ttgccgcgtt acttttgatc tgtgcaggta tcgcaattcg ttctctccct tcgccttact
     3241 tattatttgg cggtacagcg gtcattggcg gtgggattgc attaggcaat gtcttactgc
     3301 caggattaat taaacgcgat ttccctcatt ccgtcgccag acttaccggc gcatattccc
     3361 tgacaatggg agctgcagcg gcactgggat cggctatggt cgtgccgctg gctttgaacg
     3421 gttttggctg gcaaggcgcg ttgttcatgc tgatgtgttt tcctctgctg gctctttttt
     3481 tatggctgcc acagtggcga agtcagcaac atgcaaattt gagtacctca cgcgccttac
     3541 atactcgggg tatctggcgt tcgccacttg cctggcaggt aacattgttt cttgggatca
     3601 actcactggt ctattacgtg attattggct ggcttccggc gatcctcatc agtcacggct
     3661 atagcgaagc acaggcgggt tcactgcatg gtttgctgca actagccaca gcagctcccg
     3721 gtttgctgat cgcacttttc ttacatcatg tgaaagacca gcgtggtatt gcagcgttcg
     3781 ttgccttgat gtgcgcagtg ggcgcggttg ggctctgctt tatgccagcg cacgcgatca
     3841 cctggactct gcttttcggt tttggttccg gcgcaacaat gatactgggg ttgacgttca
     3901 ttggtcttcg gcaagttctg cgcatcaggc ggcggcactc tcggggatgg cacaatccgt
     3961 cgggtatttg ctggcagcct gtgggccgcc gctaatgggt aaaatacacg atgctaacgg
     4021 taactggtcc gtaccactga tgggtgttgc aatactttca ctactgatgg cgattttcgg
     4081 actttgcgcc gggagagaca aagaaattcg ctaatatccg gtgctatagt gacgtgacaa
     4141 atcttgcgtg aaagggagaa caaacacgat gaatattcag tgcaaacgcg tttatgatcc
     4201 ggctgaacag agcgatggtt atcgcgtact ggtcgaccgc ctctggccgc gcggtatcaa
     4261 aaaaaccgat ttagcccttg atgagtggga taaagaaatc acgccgtcaa cggaactgcg
     4321 caaagccttt cacggcgaag tcgtcgattt tgcaaccttt cgcgagcaat atcgcgcaga
     4381 actggcgcaa cacgagcaag aaggaaagcg gctggcggac atcgccaaaa aacagccgct
     4441 gaccctgctc tacgcagcaa aaaacaccac gcagaaccat gcgctggtgc tggccgactg
     4501 gctacgtagc ttgtgatttt agtacggcat ccggcggtta tttttcacca gccggatggt
     4561 cccgtcgcca caatgcccat tcatcaatcg tttcaccgcc cggtaatttg caattgttgc
     4621 tgaccccttg cgctgtctgc actggaatga gcgtcccgcc cttctgctgg caatagaccg
     4681 acgccggatt tgccatacca atctgcggcg gtttaggtgc ttctggctga gaaggggttg
     4741 aacaaccagc caggaccagc aagcaaggca gaacaaaact gataattttc atttattgat
     4801 ctcacatatt tatccaagct tagagtatcg cggtatcgtt ttgttttgca gcactatttt
     4861 tattacattc actcaaaaca tattacgtct tgtttcatct ttgttgatga tgttttatca
     4921 tgcctgcaaa gattaaataa tcagcactta cccgccgtat cctggagttg ttccgtgtca
     4981 gatcagatta tcgcccgcgt ctcgcaatcc cttgccaaag aacagtcact ggaaagtctg
     5041 gtccgacagc ttctggagat gctggaaatg gtcactgata tggaatcaac ctacctgacc
     5101 aaagtggatg tcgaagcgcg cctgcagcat attatgtttg cccgtaacag ccagaaaatg
     5161 cacatcccgg agaattttac cgtcttgtgg gattactcgt tatgcaaacg cgccattgat
     5221 gaaaactgct ttttcagcga tgaagtccct gaccgttggg gcgactgtat tgcggcacgc
     5281 aatcttggca tcaccacatt tctgagcacg ccaattcact taccggatgg atcattctat
     5341 ggcacgcttt gcgccgccag cagtgagaag cgccagtgga gtgaacgcgc ggaacaggtt
     5401 ttacagttat tcgccggact gattgcacaa tatattcaaa aagaggcact ggttgaacag
     5461 ctgcacgaag ccaatgctgc gctgattgcg caatcgtata ccgactcgtt aaccgggcta
     5521 ccgaatcggc gggcgatttt tgaaaatctg acgacgctgt tttccctcgc ccggcatctt
     5581 aaccataaga taatgatcgc gtttatcgat ctggataact tcaaattaat caatgatcgt
     5641 tttggtcata atagtggcga tctgtttctc attcaggttg gcgagcgcct taatacgctc
     5701 cagcaaaatg gcgaagttat tggtcgtctc ggtggtgatg agtttttggt tgtttcacta
     5761 aacaacgaga atgcggatat ttcgtcgctg cgagaacgta ttcaacagca aatacgtgga
     5821 gaatatcact taggtgatgt tgatttgtat tatcccggtg ccagtcttgg catagtagaa
     5881 gtcgatcctg aaacaaccga tgcagacagt gccctgcatg ctgccgatat tgcgatgtat
     5941 caggagaaaa aacacaaaca gaaaacacct tttgtcgcgc atccagcgct acattcctga
     6001 ggcgtattca catccttttg attggtgata acatgcgaat cggtattatt tttccggttg
     6061 taatcttcat tacagcggtc gtatttttag catggttttt tattggctgc tatgctgccc
     6121 cgggagcata aagatgaaaa aaacaacgat tattatgatg ggtgtggcga ttattgtcgt
     6181 actcggcact gagctgggat ggtggtaacg tcacctctaa aaaatagcaa aggctgcctg
     6241 tgtgcagcct ttgtgcaatt taagcgttaa cttttaatct tcctgtagat aaatagcacg
     6301 acaatcgcac caataacggc tgaagcggcg cacgaaaaac gctttcgcgt ttttcgtgcg
     6361 ccgcttcacc cctggttcag catttcgtct gcagtctgaa ccgcaggcaa ccatttttca
     6421 gcaagtctgc gctgggaatc gctggcttta tcccatttgc tccacagtgc ctcacgatcg
     6481 taatcgtgcg cttccagcgt ggggcgggcg atttcaaaat acccctagct tgtttctatt
     6541 tgctcagatt ttaataacaa ctcagcacgt gctgccgctg gtagaactga tatatgaaat
     6601 tccaaagcct ttgaacccgt acgcttgcga cgtagttctg gatgattggc tgcaagcttt
     6661 tcaagattgc ggcgaatact cacctcatgt ttaggaaagc atgacattcc
//
LOCUS       DAJTUI010000160         6686 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTUI010000160 DAJTUI010000000
VERSION     DAJTUI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.160"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(211..1092)
                     /gene="narH"
                     /locus_tag="OO464_003805"
                     /pseudo
     CDS             complement(211..1092)
                     /gene="narH"
                     /locus_tag="OO464_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
     gene            complement(1089..4829)
                     /gene="narZ"
                     /locus_tag="OO464_003806"
                     /pseudo
     CDS             complement(1089..4829)
                     /gene="narZ"
                     /locus_tag="OO464_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            complement(4911..6299)
                     /gene="narU"
                     /locus_tag="OO464_003807"
     CDS             complement(4911..6299)
                     /gene="narU"
                     /locus_tag="OO464_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR6228776.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
BASE COUNT         1601 a         1735 c         1758 g         1592 t
ORIGIN      
        1 aagcgcagaa cttctgcgtc ttcttcgtca aaaaaatcat cttcgtgaag cttcacgaca
       61 tagcggggaa gtttgcttct ttgagaggcg ggtttacgtt tacggggttt agctgaacgg
      121 gccatataac cacctgaaag acaatgacat tgcctgtttt tataacggta attgcagacc
      181 atgacaagcc gcagccgtca ggctgcctac tcgatagagg tcaacttcgc gctcggtgct
      241 cgacgcttcc tcgatgcggt cggcgtcgta aagcagcacg cccagatacc ggatgcgacc
      301 cacgcaggtt tctgagcaca cggtgggttg gcctgactca attcgcggat aacagaagat
      361 acatttttct gacttgccgc ttttccagtt gaagtagatt tttttgtacg gacaaccgct
      421 aatgcacaaa cgccagccac ggcatttatc ctgatcaatc agcacaatgc cgtcttcttc
      481 gcgtttgtag atagcgccgc ttgggcaggt cgccacgcag ctgggattga ggcagtgttc
      541 gcacaggcgc ggcaggtaca tcatgaaggt gttttcaaac tgcccgtaca tctccttttg
      601 catggcctcg aagttgcggt cgcgggcacg tttttcaaac tcgccgccca gcagttcttc
      661 ccagtttggg ccccagatca ctttgtccat ccgtttgccg tcaatcagtg aacgcgggcg
      721 agcagtaggg atatgtttgc cttccggtgt gctatgcaaa tgttcgtagt cgaaggtgaa
      781 aggttcgtag taatcatcaa tctgctgcgg caccaccggg ttggcgaaga ttttggttat
      841 tacgcccatc ttgctgccca gacgcggacg gatcttgcca ttcacatcgc ggacccagcc
      901 gccttgccac tcttcctgat cttcccagtt tttcggataa ccaatgcccg gtttggtttc
      961 gacgttgtta aaccatgcgt actccatgcc ttcgcgtccg gtccagacgt tgttacaggt
     1021 caccgaacag gtatggcagc cgatacattt atcgaggtta agcaccatgc cgacttgcga
     1081 acgtattttc attttttcgc ctcctgtacc tgatcgcgac cttcatcatc cagccagtta
     1141 acgttcttca tcttgcggat catgatgaat tcgtcgcggt tcgaaccgac ggtgccgtag
     1201 tagttaaagc cccaggccag ctgcgcgtaa ccgccaatca tatgcgtcgg tttcgggcaa
     1261 acgcgggtga ccgagttatg aatgccgccg cgcatgccag ttacttccga accaggaata
     1321 ttcataatgc gttcctgggc gtgatacatc atggtcatgc ccggcggtac acgttggctg
     1381 accaccgcgc gggcagtcag cgcgccgttg gcgttaaata cttccaccca gtcgttatcg
     1441 acaatggtca gttctcgggc atctgtttcg ctgatccaga caatcggtcc accgcgagag
     1501 agcgtcagca ttagcaggtt ttcactgtag gttgagtgaa tgccccattt ctggtgcggc
     1561 gtcaggaagt taagtgcttt ttcctggaag ccgtttggcg gtatctggcg catctcactg
     1621 acgctacggg tgtcgatagg cgggcgataa gccaccaggc tttcaccaaa agcacgcatc
     1681 cacggatgat cctgatagag ctgctggcgt cccgacagcg tgcgccacgg aattaactca
     1741 tgaacgttgg tgtatcccgc gttataggat acgtgatcgc tttccagacc ggaccatgtg
     1801 gggctggaga taattttacg cggctgcgcc tgaatatcgc gaaagcgaat cttctcgtcc
     1861 tctttgtgca gcgccagatg ggtatgttcg cgcccggtga tctcgcccag cgcctgccac
     1921 gctttgactg caacatgacc gttggtttcc ggtgccagtg ccagaatcac ttctgaagcg
     1981 tcaatggcgg tgtcaatcag cggacgcccc tgtgccgggc catcacgctt ggtgtaattg
     2041 agtttgccga ggaaatcgat ttcatcctgg gtattccacg aaatcccttt accgccattg
     2101 ccaagtttgt ccatcaatgg cccgagcgag gtaaagcgtt catacgtagc agggtagtcg
     2161 cgctccaccg ccacaatatt cggcgcggtt ttacccggga tcagatcgca ttcgcctttg
     2221 cgccagtcga gcacttcaca cggctgtgag agctctgccg gagagtcatg cagcagtggt
     2281 tgtaatacca cgtcggtttc tttgccaaga tggcccacgc acacttgcga aaatgctttg
     2341 gcaatacctt tgtagatttc ccagtcgctg cgtgattccc aggccggatc gaccgccgca
     2401 gaaagcggat gaataaatgg atgcatatcc gaggtgttca tatcgtcttt ttcataccag
     2461 gtggcggtgg gcagaacgat atcggagaac aggcaggtac tggacatgcg gaagtcgagc
     2521 gtcaccagca ggtcgagctt gccttcaatc gccgcagttt gccactcgac ttcttccggt
     2581 ttgatcccgt cgctggcacc gagttcctcg ccctgaatcc cgctttcggt ccccagcaga
     2641 tacttctgta tatactcgtg gcctttgccg gaggagtcaa gcaggttaga gcgccagaca
     2701 aacaaattac gcggatgatt gctgccgcta tctggttgtt cgcaggccat acgtaaatcg
     2761 cccgatttca gcgcctgggc ggtaaattct gtgggggata atccggcctt gtcggcttca
     2821 gctttaatcc cgagcgggtt acgccccagc tgcggcgcag aaggtagcca gcccatacgt
     2881 tcggcgcgaa cgttgaaatc aatcagatga ccgctgtact tactggcatc ggcgagcggt
     2941 gaaagtaact cctgagcaga gactttttca tagcgccatt ggctggaatg attgtagaaa
     3001 aacgaggtgc tgttcatctg gcgcggtggt cggttccagt cgagcgcaaa ggccagtggc
     3061 aaccagccgg tttgtgggcg cagtttttcc tggccgacat agtgtgccca gccgccaccg
     3121 ctttgcccga cacaaccgca gaagatcagc atattgatca tcccgcggta gttcatgtcc
     3181 atgtgatacc agtggttaac acctgcgccg aggataatca ttgagcgccc atgcgtttta
     3241 tgggcggtat cggcaaattc gcgggcgatg gtttcaatat actggcgcgg tacgccggta
     3301 atttgctcac cccaggctgg ggtgtacggt ttgatttcag cgtaatcttt cgcgctgttt
     3361 tcatcttcca gcccgcgatc gaggccgtaa ttcgccagta ccaaatcata aacgctgacc
     3421 accggacagg tgttgccatc gactaacgtc aggtttttaa cgggcaattg gcgcaccagc
     3481 accggattgt gttttacgct gcgaaaatgc ggattttcaa tgccgccaaa gtaggggaag
     3541 gccacgcctg caacagcgtc atgttgaccg agcagggtta acgacaattc ggtttccgta
     3601 ccggcggcaa tggattccag attccatttg cctttttctc cccagcggaa accaatcgaa
     3661 ccgttcggca ctaccaattc acctgcggta ttaactgcta cggttttcca ctgcggattg
     3721 ttgctttcgc ccagtccatc caccaggtca gatgcgcgga tcatgcgccc gggaacgtag
     3781 ctaccatcgt cgcgaggctc cagcattacc agcatcggca tgtcgctgta gcggcggcag
     3841 tagttgataa agtagtcgct gggattatcg agatgaaact cttttaaaat cacatgcccc
     3901 attgccatcg ccagggcgct atcagtgcct tgtttcggtg ccagccactg gtcgcacaat
     3961 ttggccactt cagagtagtc aggggtaatg gcgatggttt tagtgccttt gtagcgtact
     4021 tcggtaaaga agtgggcgtc cggcgtacgt gtctgcggta cgttagaccc ccaagcgatg
     4081 atgtagctgg agttatacca gtcggcagat tccggtacgt cggtttgctc gccccaggtc
     4141 atcggcgagg cgggcggcag gtcgcaatac cagtcgtaga aactttaaca ggtgccgcca
     4201 agcagcgaca gataacgcgt tccggcggcg taagaaacca tcgacatcgc cgggatcggc
     4261 gagaaaccgg caacgcgatc cgggccgtag gttttgatgg tccagacgtt agcggcggca
     4321 atcagctggt ttagttcctg ccagttggag cggataaacc cgccgcgccc acgcacttgt
     4381 ttgtagctca ggcacttttg cggatcattc ataatcgatg cccacgccag taccggatcg
     4441 ctgtgttgtt tgagggcttc gcgccacagt tcaatcagtc gtttacgaat gagcgggtat
     4501 ttcaggcggt tagcgctgta aagataccag gagtaacttg cgccacgcgg gcagccgcga
     4561 ggttcatgat tgggcaggtc agggcgagtg cgcgggtagt cggtctgttg gatttcccag
     4621 gtcaccagac catttttaac gtagattttc cagctacagg agcctgtaca gttaacgccg
     4681 tgggtggaac gcacgatttt gtcgaactgc caacgctggc gatagctgtc ctcccagtcg
     4741 cggttgctat gcatcacctg tccgtgacca tcggcaaagg tttcgccctt ttgtttgaag
     4801 tagcgaaagc gatccaaaag tttactcatg acatgactcc tgctccagga atgaattggg
     4861 atctgatgcc ttgtcggatg cgacatggtt gtacatcagg catcgatctc ttatttttgg
     4921 ctgaacttcc gccgaccata aaccagccag gtcagcagca cacaaacgat gtagaagatt
     4981 aaaaacactt tcatcgcgcc gaccggagag ccggtcatat tgagcgacat gccaaatgcc
     5041 tgcggaataa aaaagccgcc cactgcgcca atggctgaga taaagcccag cgttgccgcc
     5101 gtttcggtga cggcttctct ctgagcttgc tcatcactac cgcctttcat cttcacccga
     5161 taaatggtta tctggcgaaa gatgacggcg atcatctgga aagtagaacc acttcccaga
     5221 cccgcggtca gaaacagccc cataaatacg gcgtaaaagg cgatgaaatt accggagcct
     5281 gtgcccggta aggtaaggaa cagcagggca ctgaaaatcg ccataaaaat aaagttgatc
     5341 aacgtcaccc gcacgccgcc gaatttatcg gaaatagcac caccaaccga ccgcgcgatg
     5401 gcaccaataa atgggccaaa gaacgccagg cgcagaatat tcacatccgg gaactgggtt
     5461 tttgccagca tggcaaaacc cgcagaaaaa ccgataaacg aaccgaaggt ggcaaggtaa
     5521 agcaggctca gcagccagag atgcaggcgt tgtaagacag ggagctggtc ggcaattgag
     5581 gcgcgtgaac tggcgatatc attcatccct gaccaggcgg cgatcgtggc aatcgccagt
     5641 agcggtaccc aaatccatgc ggcattcgcc agcgacatca ccgaaccgtc ggcctgcggt
     5701 acgccattga cgccgagaaa ggcaaataca ggtacaaaaa tgaccagcgg tgcaaccagc
     5761 tgcattacac ttacacctaa gtttcctaat ccgccattaa tcccaagagc gctcccttgt
     5821 ttggcttttg gaaagaagaa actgatattg cccatgctcg aagcaaagtt tgcacctgca
     5881 aaaccgcata gcaaagcgat aacgataaat atcccaaaag gagtattcgg attttgcacg
     5941 gcaattccga gccagacgca aggaataatc aggattgcag tactaaaaac cggccatcgg
     6001 cgtccaccga atataggcac cataaaggag tagggaacac gcaataatgc gccagaaacg
     6061 gagggtaatg cggttaataa aaagagttga tcggtagtaa aattaaaacc gattttattg
     6121 agattaacgg taactgcgct aaatagcatc cagacacaga aggcaagaag tagacaactg
     6181 actgatatcc agaggtttct tcgagcaata tgttttcctt tattttccca gaaggccgga
     6241 ttttctggtt tccagtcgcg taaaagataa cgactatttt tctcattttg cagtgccata
     6301 ttgttcctca tatgcacaca ttggtaatga aaaaagacaa aacaggaggt aaggcgcaat
     6361 agccagttat tagaattaag gatgaattgg gtgaagtgct gattgaaaga atagataaga
     6421 aagcgtaacc gcgggggcag aatggggatt aagtagccag atatgtgtta ctaaatgtaa
     6481 ctaacaagct aaccttcaaa cgggggttaa tctttgaacg tatgtcatgt ttcaaccctt
     6541 cagatcgtga atctaaaggg ttacatatta actatattct caacaagacc agacacttat
     6601 agaattagtg atgatttatt cactaaaaag gccatgaatt ggaggaaaac gatctgtcca
     6661 gaaagtctat cggttcctct cttatt
//
LOCUS       DAJTUI010000161         6424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTUI010000161 DAJTUI010000000
VERSION     DAJTUI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.161"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..208
                     /locus_tag="OO464_003808"
     CDS             <1..208
                     /locus_tag="OO464_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6228777.1"
                     /translation="TGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWV
                     REQLNKKHSNVVACALANKLARIA"
     gene            complement(547..1317)
                     /locus_tag="OO464_003809"
     CDS             complement(547..1317)
                     /locus_tag="OO464_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228778.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1362..1796)
                     /locus_tag="OO464_003810"
                     /pseudo
     CDS             complement(1362..1796)
                     /locus_tag="OO464_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(1808..2029)
                     /locus_tag="OO464_003811"
     CDS             complement(1808..2029)
                     /locus_tag="OO464_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228779.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2030..2713)
                     /locus_tag="OO464_003812"
     CDS             complement(2030..2713)
                     /locus_tag="OO464_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6228780.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(2777..3088)
                     /locus_tag="OO464_003813"
     CDS             complement(2777..3088)
                     /locus_tag="OO464_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228781.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3085..3987)
                     /locus_tag="OO464_003814"
     CDS             complement(3085..3987)
                     /locus_tag="OO464_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR6228782.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4427..5386
                     /locus_tag="OO464_003815"
     CDS             4427..5386
                     /locus_tag="OO464_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR6228783.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5386..5781
                     /locus_tag="OO464_003816"
     CDS             5386..5781
                     /locus_tag="OO464_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR6228784.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            5964..6344
                     /locus_tag="OO464_003817"
     CDS             5964..6344
                     /locus_tag="OO464_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR6228785.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1756 a         1666 c         1556 g         1446 t
ORIGIN      
        1 cactggagga aaatcgactc tgttgggtat cagtaagcga ggtgataaaa atttacgccg
       61 gttactggtt cagtgtgccc gctcattcat gatgcagctt gaacgccagc atggaaaact
      121 tgctgagtgg gttcgggagc aactcaacaa gaagcattcg aatgtggttg cctgcgctct
      181 ggcaaataaa ctggcccgaa ttgcctaggc aatcactact cagcaaaaag aatatcaggc
      241 ctgatatata gatgctgaca aaactcaatt agcttattgc aaacaataag atacaagtta
      301 ccgtctggtt ttgcgaagat tgattattga tgacatgaac ggcaaatcga cctgattaaa
      361 aacctaacag gggaaaatgg ctcactgaag ccgaggggat ttttaggatt atcaggtgcg
      421 gaactcatcg aggcgcgggc atcccttgcc cacagagacg ccggatagat ttaagcaagc
      481 ccaccattaa tcaaaaatca gtgttgcaaa aacgggggtg accatagatt ttccccgcca
      541 tcgtcatcat ttccggattt ttgccagggc atccgccatg ctgcggattt cggcccgggc
      601 ttcccggaca acttcccgca gaacaccacg gtcaaaccac cggcggggcg gttcatccgg
      661 tatacgcagc acatcgcagg ccagcccact ggacaggata tgctcaatac aatcgctgac
      721 cagatacatt tccaccgccg aaaaacgtgc gccccggttc aggatggcat cgagcaccac
      781 gggcaggttt tcatcgtctt catcgagcca gcaggggtga tcgctttcct tcacgaccag
      841 gcgaaagcgg cgcgtctggc gggtcagcaa cgtctgaccg cttgtggtca gcgccgcccg
      901 gatacgccgc gcatcttctg ccccccggca actgacactg catactgcat cgcgcaccgc
      961 atcaagaact gccctgattt cgtcttcggt cagccattca ccggcatacg ggaaagacac
     1021 ctgcgttccg tccgccggaa gtaccggcag cgactgccac tgactgccgg acagaacaag
     1081 ttccgctccg gttccggtca gtttctgaat ggcggaggcc aggtgcggcg gcagcacgcc
     1141 atcgtggcgg ttaacgagta acgtcgccag ccggggcgca tccagccgca cgtaaatgct
     1201 gatccagccc ggatagttcg ggttacattg gtcaacatcc gtctcttccg gccagccctg
     1261 cgccagcagc aggttacggg cgtgacgaca aatcagagca ttcagcgttt cattcataat
     1321 tcatgaactc cctgtatcgc cgcccacagg cggcgataac atcagtcgtt ttcgtgtgtg
     1381 ttgtttttca gcaggctgcg ggcctgatca accatcgcct gtcggcgggt aacgtattcc
     1441 gggaggggga aaccgtgatc ccgggcagcc cattcacgtt tccacagtgc ctgttcagcg
     1501 atctgagtca gggtctccag cagttcagca gggacggtca cctggcggtt ttcgcgcacg
     1561 ttgctgataa tctccatcgc cgcggcggaa gaaataccgg actcaatccg ctagtcttcc
     1621 gggatgtcct ccaggtgcgc caggacggtc ctgatttcat gatcggacag ggaaatatcc
     1681 attccgtcag cgagggcctg aatctcttcc ggggaccaga taaccagcat cagcggcgtg
     1741 tctcccgaat actttgctgc cagcgcccgg catagcgttt cgcatgttcc gtacatactc
     1801 tctcccctca atctttgaag caggaatcca gttccagaat ttcggtatat gcctccatca
     1861 agaggcggtt tgcgcgaaac aacgtctgac gcaccagacg ttccggcagg gtgtcagcat
     1921 taaagctgta ccgccgctcc atctcattca tggttgtacg caggtcgcac atggcattgg
     1981 ccacctccgc cacttcggcg cgaagttttt cgtgtccggc atagttcatt tacgcagcct
     2041 ccggcataaa ccagtcatca ttcgcggccc cctgctgcat ggcccgctgt acggcagcca
     2101 gtcgttgctg tccggcacgg tgatactgct caagcagctc gataccgata taccggcgtc
     2161 cggactggag ggcggcgacg caggttgaac cgctgcctgc aaacgggtcc agcactatcg
     2221 cattcgggtg tgtgaagctc tcaatcagcg gttgcaggct ggtaacgggc ttttccgtcg
     2281 ggtgatggcg gttgcccgaa tatttccagc ccaacacgtc cggcagcggg ttttgtggca
     2341 gacgtggacg gccttttgcc aggatgtagg cgcattcgtg gcgatagccc acatatgcgg
     2401 ccttcgatgt gtagtttttg gtgaagacca ggtgaccaac aacgctgaat cccgcatttt
     2461 tccaggcaga cataaagcga tcgacgcggt tccagccata gaagctcacc atcagcgcgt
     2521 cttttttgag tacgcggtac atttcattac aggccggttg cagccattca tcagttttat
     2581 caccagcaat ggtacgcccc tgacggtcac gaaaaccgac gagatatggc ggatcggtga
     2641 ggatgaaatc aatggcgtta tcaggaatac gggccataac atcgatgcag ttaccgagga
     2701 caaaacggga cataacagat ccttctctta cagagggcac ccctgtggtg cccgttcccc
     2761 gctgcctgtg ggctgttcat cgcttccggt aacgggtcag gccgaccgtg gaataccgca
     2821 gccgtcgtct ttttgacagc aaggttatgc cgcgaaagcg gcttgaaggg cgttcatcag
     2881 aaaagggcga cgggctggcg tacactgtca ccgacaccag ccgggagcgg taaaagcgcc
     2941 cgtcccgtgc ccgccggacg cgggacactg accacaggca gcgtttatgc cgcgccgcgt
     3001 cagccggcgc agtaaaaaga ggagcacggc gacatccttt gtcggaaaag aaacgggcgg
     3061 ttgcccgccc gtctccgtca tacttcatcc gccataatgc gccacattgt cgactatcca
     3121 cgcaggtccg gtgacttccg gctgctcatc gtcatctgtc ggggaagacc attcaagttc
     3181 cccccacagc acattgacga gctctccacg ctcatacaac tgccagccac agaaatcaca
     3241 gccctgttcg gcataccagt gctccagcgt gcagctgaaa cggcggctta attctgcaat
     3301 aacgtcgctt tccggctgac accacggagt gtcgaaatcc acctgaatga agttttctcc
     3361 ctgactgctg atattcaggt catatccaca aggccacttc acgccatacc gttccgtata
     3421 ccagtgatat gcagaaggaa caccgttcag aacaccacca ttaaagccgt tcacttcaac
     3481 atccaggcgg gtcggcagaa ccgtcagcag gtcaaacggc atggtttttt ccggcactac
     3541 gttgtcacac agacggttcc accacaccga tacatcctcg ttaccccata tgtcacacca
     3601 gtcacctctt tttgcggtga aatgcacagt gatggtttcc ctggcatcat ccggtaatcc
     3661 ttcccaacga cgctggccag taccgctctg taaccagagt tcatgcagca tcaggcagtt
     3721 ttgttcatcc agcaacacac cgttctgcag gtgtgtcagc cagcgggtga acgcgatatt
     3781 ttccgtcgat acagcaccac gtccggcagc cgtcactccg gggcactgtt cagagcggat
     3841 attttcagtg atctgcagaa gtccggcact tccggccaga aacagctgaa taccttcatt
     3901 tgtggcgcgg cgataaagcg gtgtgactgc accgctggcc agtcgtttaa tctcagcgat
     3961 ctgtgccggc tcaccaggaa aatacatacg attcgagcac cagtttggca tgggaattct
     4021 cctctcacat gcggcctcca tcaggccgtg ccgcacctgt aaccgggcga ccgcatacga
     4081 atgcaggtgc ggccccgaag ccgcagcgcg tggaggagga atgacgaaac gtgaccgggt
     4141 gaggaggccg gcgacagcga cacacttgca tcggatgcag cccggttaac gtgccggcac
     4201 ggcctggtta aatagtatcc gtctcagttc gtggcgcaaa atgagaaacc aataagaact
     4261 caaaatgaac cacaacaaga acataaagag actctaaaag agcaacaccg tttgacatag
     4321 actcaaaatg agtcacaata agaaacaata aaaaactcaa aaagatgcac cgacataaag
     4381 gatgctcaat ttgagataaa cacaaactca aaacgaggca aaaagaatgc taaaagtatc
     4441 atgcgatgat ggttcaacta acgtaaaact ggcctggctt gaagatggcg aggtaagaac
     4501 cagtctgtca ggaaacagct ttaaagaagg ctggaacccg ggactgttta atgccggcaa
     4561 agtttacaat tacgtcgtgg atgaaaaaaa atacacctat gatttgggaa gcactgctgt
     4621 cataggtaca acacatgtca gttatcaata cagcaccacc aatctgcttg ccatacatca
     4681 tgcactgctg acatccggcc ttcagccaca ggatgtagaa ctgacagtaa cattacctgt
     4741 gaccgaattc tttgataatg acaatcaacc aaatgaagaa agaatagaaa gaaaaaaggc
     4801 aaatgttctc cgtgagatat ctctgaataa aggggaaaca tttaaaatta aaaaagttaa
     4861 tgtaatgcct gaatctttgc cagctgcatt tgaatcacta aaaaaagata aagtaaacaa
     4921 acttgaacgc agcctgatta tcgatcttgg aggaaccact ctggattgtg gtttaattct
     4981 cggtgcattt gaaggcattt ctgaaatccg tggctattca gaaatcggga catcccgcat
     5041 cactcatacc gtcatgaatg cgctgacgaa agcctccaca ccatgtaatt attttattgc
     5101 tgatgagtta ataaaaaaca gacacgataa tgaatatctc cagacattaa taaatgatgt
     5161 agctgaaata aaaaacattt ctcatgtaat agacagagaa gtgaaatctc tggcagaaag
     5221 catcaggcag gaaatttcaa cattctcagg aatgaaccga atctatctca ctggcggagg
     5281 agccgagctt atttaccctc acataaagca atatttcccg aatctgaaag tgaacaaggt
     5341 ggatgaacct cagtttgcat tggtgaaagc aatggtgcat gcgtaatgga aagcagtgat
     5401 ccaaaaaaaa gaaagaaagt tgtggcatac ctgcacccgg cactttatcc gcaggacaac
     5461 ctgacacaac aaacaataga ctctctccca gtacaaatgc ggggagattt ctacagacaa
     5521 tcccttattt gcggagcagc tctttattct gttgcccccc gactgttaac gctaatcagc
     5581 gtttttttca gtgagaaaat cactgcagaa aatctggtga aacttataga acaaacaaca
     5641 ggatacacat ccacatcaat tgatataagt gtgttaaaaa acatcataga ggcatcttca
     5701 gaaaacaaat cagagagcat cacttcaaaa gatgatttcg aagaacaaac cagacgcaat
     5761 ctttctatgt taaaaaaata ataaaagaag agcaggttca gtcaattacc tgctcttctt
     5821 ttatacatac taaaaaatca gatttttacg aatattcaga ttatcataaa aaatgcaaca
     5881 gacaggaatg caataatttt gcattatctc gttgcttgta atgcaaatat gcattattgt
     5941 cagtgtgtga caataacaga gagatgacga tggcgaccat taacgcacgt attgatgatg
     6001 atataaagaa ccaagcagat gaagtgctga agctgatgaa tatcagtcaa actcaggcta
     6061 ttgcagcttt ttatcagtat attactgaac aaaagaaact gccatttgtg atcacctcaa
     6121 ttgtaaaaac gccgcatgat ttattacgtg aaagtacaga catgttagct gaagctcttg
     6181 cagtgatttc caatctacag gtgtggactg aacagcagga tgggattgga aaagcaaagc
     6241 taatggagta ttaccgccgc ctcgacgcac tgtactgttg tgcaaaagag aaaataggtc
     6301 ttctttcaga taaccgggat gctgaactag gctgtgtccc ttaatcatct gagttatagt
     6361 aactgtctgt ttacacaaca tattgaacta tggctcgcta cgaccttccc gatgaggcat
     6421 ggac
//
LOCUS       DAJTUI010000162         6255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTUI010000162 DAJTUI010000000
VERSION     DAJTUI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.162"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..312
                     /locus_tag="OO464_003818"
     CDS             <1..312
                     /locus_tag="OO464_003818"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6228786.1"
                     /translation="VAMIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKT
                     HDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNV
                     R"
     gene            complement(388..852)
                     /locus_tag="OO464_003819"
     CDS             complement(388..852)
                     /locus_tag="OO464_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6228787.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(849..1724)
                     /gene="nanK"
                     /locus_tag="OO464_003820"
     CDS             complement(849..1724)
                     /gene="nanK"
                     /locus_tag="OO464_003820"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6228788.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1721..2410)
                     /locus_tag="OO464_003821"
     CDS             complement(1721..2410)
                     /locus_tag="OO464_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6228789.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="OO464_003822"
     CDS             complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="OO464_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6228790.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="OO464_003823"
     CDS             complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="OO464_003823"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6228791.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="OO464_003824"
     CDS             complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="OO464_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6228792.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1585 a         1695 c         1552 g         1423 t
ORIGIN      
        1 gttgccatga tttacattga acagcttgaa ttaatccata aaagtggtga tgtgttgtat
       61 ccagtaaaaa taaccagaaa atcatcagga aaaactgcct tccaccttgt tccattcggc
      121 ttaaataaga ctcacgacct tctagaggtg gaagatgctt cagaagcaat tcggctggtg
      181 attgatgaac gtcattccat tcgctgcagt acacttacag caactattac gaataaaaag
      241 ggcaagcgaa ttaagcggac aggtatatac agtatcaagg gtgttaacat aaaagaatac
      301 aacgtcaggt aatggcacag agcggtccgt cagttcaggt ttggctctgt gccaaagatg
      361 ggtcaggtca cacctgatct aataaattta agccattaaa tcagccttaa ccttcaccac
      421 caccttttta atctcacccg gctcgccaac aacgcatccc ggcttatgcg gctctcctgg
      481 cataaacacg gcgaacattc ccggtttcag aatgatggtt tgctcgtttt caatggcgct
      541 acaaagctgg taatcatctt catggtggaa ctcttcacat tggcgcgcag tgcctgccat
      601 gccaaacaga atccgttcct caccgtttaa taacagctgg atatcaatgt attgctcgtg
      661 caattccgct tttttctcga cgggcgattg agtgttaaac gtcatgacat tcataaaaat
      721 attgtcgccc tgtaattcgt aacgacccgg cgctttttct tgcggtctgg cagctaaggc
      781 cagcgttaac gcgtcctgta acgcaggatg taacccagca gacggtaatg actgtacttc
      841 acccatcatc ataatttttc tccctgggcc aacagcgcag ccccaagtaa acctgcatca
      901 tggcggtaat gcgccgccag taaatcaaca tgaaatgccg ctggctcctg cgccagatac
      961 atttccacca acgccagata cccttctgcc agaccaacgc tgccaccaac caccacgcac
     1021 tggcaatcag ttgtggcttt aatatcagcg atcagccttg caagcgtacg tgcggagcga
     1081 tgaatcagtt gctgcgcctg ctcatcgccc tgcccggcgc gtgtgaaaat agttctcgca
     1141 tccgcgccag ccagctcgcc ctgcgctgcc gccgcaatgc cgcgaccaga agcaattgct
     1201 tccacgcaac ctgtgcgtcc acagccgcag actgggccgt gtggatcggc aagcgtatgc
     1261 ccgatgtgcc ccgccagacc gccagggccg gtaagcagtt tgccgccgct cactacaccg
     1321 ccgccgacgc cagtggaaac ggtgataaag accatatcgg ttatatcgcc ttccagcgcc
     1381 tgatactctg cccatgctgc ggcctgcgcg tcgttaatgg caatggtcgg caaattggta
     1441 agttgttcca gcgttttgac taacggaaag tgtagcaatc caccgagatt atgcggattc
     1501 agcgccagca aactgccgtc gcgaataatt ccggttgaag cgatggcaac ccgctgcgca
     1561 tgagcttgca acggagagac taatgcggat aaggcatcac gcaaggcttc tggtgtctgg
     1621 ctggctggcg taggaagttc acgacgatcg cggatctgcc cgtcagcgcc aatcagcgcg
     1681 gcggcaagtt tagtaccgcc gatatcaatc gccagtatgg tcatagcacc gcctttttca
     1741 tcgctgtgtt gtaccactga caaatgtgtt caagacgcgt aatggcagaa ccgaccgtca
     1801 ccgcccacgc gccgtggcgc atcgcatctg ccgcctgagc aggcgtgttg taacgccctt
     1861 cggcaatcac ccgacatccg gcttcgctca acgttttcac cagcgccaga tccggctctt
     1921 ctggcgtttc aggcgtggta tagccagaaa gcgtagtgcc aataatttcg gctcccagct
     1981 tttggcatgc caggccgtct tccggcgttg agcagtcggt catcgccagt aaaccgtgat
     2041 gatggatacg tgccagcagc gtttcaacag gcaccggacg cgggcggtcg gtgccgtcaa
     2101 tggcgataat gtccgcgccc gcctgcgcca gcgcatcgac atcttcaata taggccgtga
     2161 tgcgtaccgg agaatcctcc agatcgcgtt tcacaattcc aataatcggc acgctcacca
     2221 ccgcacgcgt ggcttgcaga tttgccacac cttcgatgcg aatggcaacc gcgcccgcct
     2281 gctctgccgc taatgccatg gctgcgacga tttcgggttt atcgagcggg ctgtcaggaa
     2341 ccggctggca ggagacaatc aggccaccgt tagcagcgat tttttgatcc agttgtgcaa
     2401 gtaacgacat acatcttccc ttagcgaaag gcccggtaca tagaccgggc aacaggatta
     2461 acttttggtt ttgactaaat cgtttttggc gctgccaaat ggcacggcac cgctaaatgg
     2521 tttgccgtcg atagcgtcat gagtacgcaa tgcttccggg cgcagccaac gctgaacgcg
     2581 ggaaggcata tccagaccaa tcagcaggat caccacaaat gtcaggctga acgagagcga
     2641 tgccagcgca gtacccagat ccagacgttg agcgatcaac gcgccgatga ttggggccag
     2701 tgcgccaccc aatgcgccaa cgttgtaggt aaagcccagg cccgctgcac gctggtcggt
     2761 atcgaaataa ccgccaatca gtttcggtaa gatcccggcg atcccttgtc caagcatttg
     2821 ctggaagaac agtaacagac cgagcaccca gacgtttgcg ccgccaatcg caaataccgg
     2881 aataatcagc agctgcgagg ccagcaggct acaaacgtac gctttgcggg ttcccagcca
     2941 gtcaccgagg aagccaccta cgcagcatcc cacggccgcg ccaaagccac taaagaacag
     3001 cacattggcg acagtatgcg ggttataagc cagatcggtt ttcagatacg ttggcagcag
     3061 cgcctgaatc ggccatgagt agaggaaagc aaacaacacg accaccatca gcattacgcc
     3121 cgttggccag cgtttacccg tactttgcac cataaagctg ataaagattg cggcgcataa
     3181 cagcccgaga acagcgacga ttgcagcatt ttgcaggtta ccggcgaagc agaaccacag
     3241 cgcagtagcc gccgccagtg tcattacgat attggcaatg cgatgttcac cacggtagag
     3301 aatatccacc attgtgcgta ctggtgcttt acctgcgtgt ttctctttcc agtcttccgc
     3361 ttccgggatg tttttacgca gccagagagc aaagatgatt ggcaaaatgc cgataaagaa
     3421 cagcgcacgc caaccccaga ccggaaccac caggctatag acctgagcgg caacgacggc
     3481 ccccacagag aagcctgaaa tcaaaaaacc actggctttg ttacgcagat gttttggcca
     3541 gctttcaatg acataggtgg cgctggaacc gtattcaccc gccatcccca tgccgatgac
     3601 cagacgagcg ataaacatgg tgatgtagcc tggcgcaaag ccgcaggcca gcgtcccggc
     3661 cgagaagaga acgatgctgg tgaccattgc cagacgacgc ccgtagcggt cacccatagc
     3721 gccgagcatc aggccgccga accagcgaga gataaaggct gcggagatca gacttgccgc
     3781 ctgcaccgtc gtcagcccga attcaccctg tacttcggtg agtaccaggg cgattaaaac
     3841 gaaatcaaaa ccgtcaagca gatatcccaa ccaggcagcg gaaaatgcgc gccattgtgc
     3901 acggttgaga tggcgatacc acgggatatt ctgggttgta gtactcattg tgagtctccc
     3961 gcggtgggcg atgcccacac gctttggtat gaaaattgta gggtacagat gcgtttattt
     4021 cccctcaccc ggtaggggcg agcgagggga aaacaactca cccgcgctct tgcatcaact
     4081 gctgggccag cgccttcagt tctggcagat atttttcatc taccggtcca aacggtttgc
     4141 ggcacagcgg cacagaaacg acatccatat aatgcaggac agttttcagg ccgcggaata
     4201 cgcccgtttt gatcagtaaa tcaatgactt tgttgcactc agtttgcagt ttctgcgcgg
     4261 tctggatatc gccttctttc agcgccttca cgatcccctg atatcgccag cccatgatgt
     4321 tgtaagtact gccgatacca ccatcagcgc ctgccagcaa gccggaagcg aagatttcgt
     4381 cgtaaccgtt atagagcacc agatcaggat gttcacgacg gatctgttcc atctgataga
     4441 gatcgccaga agtctgtttc agcgcaccga cgccaggcaa cgtaaccagg gtgttgatct
     4501 gatccagggt cagttttacc ccactcaggg ctggaatgtt gtacaccacc atcggcaaac
     4561 catccgctga atcaataatt gcccgatagt gatcgcagtg ttcttcaaag ctgaaaggat
     4621 agtagaacgg cgtgacggcg gagacggcat cgaagccata acgtttagcc gatgccgcaa
     4681 gttgttggct ttcggcggtg ctgacgcaac cgacgtgggc gatgagttta atcttacctt
     4741 tcgcctcttc ggcgacgatt tccagtacct gttcacgctc ggaaaggctt tgtacaaagg
     4801 cttcgccggt cgaaccaccc acgtataaac cgtcgatgcc ctgctgaata ttgaactgaa
     4861 ccaggcggcg cagactcgct ttatccagtg cttgttgttg atcaaaagga gtcaggagtg
     4921 cagccattac gccacgtaaa ttcgttgcca taaatacctc tgaagtgatg cttgtctgat
     4981 aaacgatata cctttatacc tgttatacca gatcaattaa gcaacacccc atacagaaag
     5041 cttataatgc gatctgcttc actaaagtgg cattatttct ttttgttggt ggtctgaccg
     5101 aaagcgtgcc aggtagcaga gacgctgttg agatgcgatt gcaacgcacg atcggcttcg
     5161 tcaggatcat gacggcggat cgcatcaacg atcgcaatat gctgttgata actaacgttg
     5221 ttatgttcgt gcagtgcctg atcggtaacc gttgggcgtg cggcaataag ccagtcgagc
     5281 agggcaacgt ggatcgccat gaagattggg ttaccgggga tctccgccag cacgcggtgg
     5341 aaatcaacgt ctgaacgaat gaatgccgcg ttgttatcca gcgactgact gttgatttcc
     5401 agtgcttttg ccagcaaatc gatttgctca tcggtggcgt gttcagccgc atagcgcacc
     5461 aggctggatt caaagaacag acgtaattgt tcgaaatggg caatcccacc aggatgagaa
     5521 aggaaatctt tcgccatgcc ggaaagctcg ccgatgatag tgtccgcaga aggtcgagag
     5581 acacgagcgc gttcgccgtt gtttatttgc accagacctt tgcgttttaa cgctgccagc
     5641 gcttcacgca cagaaggacg cccgacgtta aagaacgcca tcagttcgcg ttcagacggt
     5701 aattgttcac cttcgccaaa ttcacgacgg cggatcatct gttccagctc ttcttccacc
     5761 atttcggaga gttttttacg cgccagcggg cggctacgca agttgcgacc aattgcaggt
     5821 gaagaatctt cggtttgcga atcaaatgcg ttcataaggc ccattctgta aggtcagtgt
     5881 gattaacatc atcagtgaca tcctatcaca ggattgaaag taggggaaaa tggcaggttt
     5941 ttctctttgt gcctcatcat taccataatt aacggaataa ttaactattg cgaaaaatta
     6001 atgtaacgca gataaacaca tcccgattga attattcata agactattca caagcattat
     6061 gaatattatg aatgtgttct tacaaaataa tcataatcgc atatttttta atgaaaaatc
     6121 acctcaccta caattaaaaa cacgacatcc gcaccataaa tagacttgca aaaaatataa
     6181 catcattgtt ttcaatctgc catttatggg attgaccgtt ttcttttgac acggagttca
     6241 acaatgggta atgct
//
LOCUS       DAJTUI010000163         6152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTUI010000163 DAJTUI010000000
VERSION     DAJTUI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.163"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..171
                     /locus_tag="OO464_003825"
     CDS             <1..171
                     /locus_tag="OO464_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6228793.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="OO464_003826"
                     /gene_synonym="chbF"
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="OO464_003826"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6228794.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="OO464_003827"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="OO464_003827"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR6228795.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="OO464_003828"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="OO464_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR6228796.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="OO464_003829"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="OO464_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR6228797.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5430..6152
                     /locus_tag="OO464_003830"
                     /pseudo
     CDS             5430..6152
                     /locus_tag="OO464_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1577 a         1310 c         1557 g         1708 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagagat
     5281 cttttttctt taattattta tttattttaa aatggttaag ttttgctaat catgatgagt
     5341 tatttttgat agttcatcct gtggcgtcgc tcttaaactt atattgtaaa tgacttagat
     5401 ttaaataaga taataatttt gttaacaata tgaggcggga gatgttttct acaaacttaa
     5461 tacagagtaa ttatggtgat ttaaatatta aaagcctggc tttcgactct tttaaagaaa
     5521 ggcttgagtc taccatggcg gcactcactt tctttatatc aacgggtcaa tgtgactgcg
     5581 atgaaatagc agagtcgaac ttcaatcgta tgattgccta tatgtctaac attaactatg
     5641 atgcctcaaa gctaggagcg ccagcactta gttttgatac gtatctgcag gacaatgtta
     5701 aatatagagt gataattaat aatctttatg gcagtgagtt tcggataaga gatattaatg
     5761 agaattttgt tgggatggat tttacctgtg aattccgacc cgaaaaaatg acgaatttga
     5821 tcaatataaa aaataggttg gttattcatt atttaaggac tatatactat gaaaaaaatt
     5881 atatacatcc tgccgggagc atttttgctg cgattcaaaa aaaatgagag tctcctaaag
     5941 tttccttcga taatatcaat gcttaatatc aatcttcttt ttaatccact aaatcttccg
     6001 ggaatgggaa gtggtgtgct tgaagatatt atgagtattc cagatagttc acttagaaaa
     6061 cgtctcgggt atgaggtttt aagcttctca ctacaggcac attcattgag ccaggaatgt
     6121 attgataagt tggacatttt tttcgctgac ga
//
LOCUS       DAJTUI010000164         6060 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTUI010000164 DAJTUI010000000
VERSION     DAJTUI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.164"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..145)
                     /locus_tag="OO464_003831"
     CDS             complement(<1..145)
                     /locus_tag="OO464_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR6228798.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQM"
     gene            381..653
                     /locus_tag="OO464_003832"
     CDS             381..653
                     /locus_tag="OO464_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR6228799.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(619..1026)
                     /gene="nudG"
                     /locus_tag="OO464_003833"
     CDS             complement(619..1026)
                     /gene="nudG"
                     /locus_tag="OO464_003833"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6228800.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1113..1733
                     /locus_tag="OO464_003834"
     CDS             1113..1733
                     /locus_tag="OO464_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6228801.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1742..3049)
                     /gene="ynjE"
                     /locus_tag="OO464_003835"
                     /pseudo
     CDS             complement(1742..3049)
                     /gene="ynjE"
                     /locus_tag="OO464_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3116..3769)
                     /locus_tag="OO464_003836"
     CDS             complement(3116..3769)
                     /locus_tag="OO464_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6228802.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3769..5240)
                     /locus_tag="OO464_003837"
                     /pseudo
     CDS             complement(3769..5240)
                     /locus_tag="OO464_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5213..6060)
                     /locus_tag="OO464_003838"
                     /pseudo
     CDS             complement(5213..6060)
                     /locus_tag="OO464_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1428 a         1622 c         1599 g         1411 t
ORIGIN      
        1 acatctggcg atattttgga ttttgttcaa gaaaaggcca gagtgtggtc attacttcac
       61 gaacggcttg cgcgaactcg gtttgattcg ggtcgcgctt ttggacatgg ttgaggaatg
      121 actccagaga atatgtctga tccatagata taaaaacctt atatattaat acgattgtgc
      181 ttatgttgtt tatgtcatgt gcttttgcag ttttcgacta taccatcaag aatgtgacgg
      241 taagcaagaa aaaaatgacc caggaaagca aattaataac gagagtaatc tcataaccaa
      301 atgcgataac tcagacgcta aagttttgcc acccggctac cgttatcagt agcagagaca
      361 ataacgttaa ggagttccct gtgagtcgag cattgttcgc cgtagtatta gcatttccat
      421 taattgcact ggctaacccg cattatcgtc cggacgtgga ggtaaacgta ccgccagaag
      481 tgttcagttc aggcgggcag tcagcgcagc cctgtactca gtgctgcgtt tatcaggatc
      541 aaaattattc tgaaggggcg gtaattaaag ccgaaggaat actgctgcaa tgccagcgtg
      601 atgacaaaac ccttagcact aatccgctgc tctggcggcg cgtaaagcca taaacgcctc
      661 taataatgga atgtcagcag gggccagcgg atattgcagc gcctcttcag gtgagcacca
      721 gaccagcgcc tgatgttcat gtgcctgtaa cgtcccgtgg aagtcgggta cgtgccaggc
      781 atgaagatgg ataatccggc ccgaaacttc tcgctgatgg ctggcaacat actcacccac
      841 agttgcttcg atgtccaatt cttcgtttaa ctcacgcacc agcgcctgct gctggctttc
      901 atccagctcg actttacctc cggcaaactc ccataatccc gcctgatcgc tctgggcggg
      961 gcgttgcgcg agtaaaattt tgccatcacg ttcaatgatg gcggcaacaa cttcaatcat
     1021 tttcataaat tacacacggg cagataagcg aaaaatgcca taatatccgc actgttgctc
     1081 aatcagaaag gctttatatt taaggagtgc ctgtgctaga ccgccatctt catccccgga
     1141 ttaaaccgtt gttgcatcag tgcgtgcggg ttcttgataa accgggcatt acgccagatg
     1201 gtttaacatt agtgggattt gccatcggcg tgctggctct gccgtttctg gcgctgggct
     1261 ggtatctggc ggcgctagtc gtcattttgt tgaacaggct gcttgatggt ctggacggtg
     1321 cgctggcgcg gcgcagaggg cttaccgacg cgggcggctt tcttgatatt tctctcgatt
     1381 ttctctttta cgcgctggtg ccgtttggct ttattcttgc tgcaccggag caaaatgcgc
     1441 tggcaggcgg ctggttgttg tttgcgttta tcggcacggg cagcagtttt ctcgcctttg
     1501 ctgcactggc agcaaaacat cagattgata accccggtta tgcacataag tcgttttact
     1561 atctgggggg attaaccgaa ggcacagaaa cgatcttact gtttgtgctg ggatgtttat
     1621 tccctgcatg gtttgcgtgg tttgcgtgga tcttcggtgc gttgtgctgg atgacgacct
     1681 ttacgcgggt gtggagtggg tatctgacgc tgaagtcgct ccagcgtcag taattgcaat
     1741 gttatttgct actgcccggg ccgcgttcac cggttgcaac cggatttttt ggatcgctgc
     1801 tccattcgta ccagccgccg tcatacacgg aaacattctt ccaacccatg gcgcgtgcgt
     1861 acataaaggt ttcggacgcg cgccagccgg tgccgcagta gaatgaaact tgctgatctg
     1921 gtttgatatt ccatgctttc cacatagcgg taatatcatc ggcgctacgc atggtgccat
     1981 ccgggttata gaaatcttcc atatgcgtcg agtcgctacc agcgtgtccc caacgtgctc
     2041 cggctatttc accttttggt ttaatatagc tgtaaccgct ggtcgtaccg ataaattctg
     2101 gccacgaacg aatgctcacc agcgatgcat cctggcgatg cagcagtcca cgggcctgtt
     2161 ccatatcgag catcaactgc ggttgtgccg ggatcttcac gccgaaatcc ggttccgctt
     2221 tcactttcgg tggcgttcct cgctcaacag gcagtcccgc gtcggaccag gtttgccagc
     2281 cgccatccag cagacgcaca tctttcacgc ctgcgtagag cataatttgc gccacacgcg
     2341 ctccagcgta tacgtcacgc ccgtacagaa tgaccgtggt gtcatggcga atgccgtgtt
     2401 ttgccagcat cgcttttagt tgttcatcag aaactttgtt ccacagcggt tcgctttcca
     2461 cttcgttggt atcgatgtag tcagcgccag gaatatggct gataaggtaa agcttaggag
     2521 cgccctaggc cgcttcaatg actttccagt caccgacagg tttcgccgta acctctttac
     2581 cttgttgcag gtcatgcagc cattgcggat aaaccagctg ctcaaagtgc ggcagttttt
     2641 gtagacggga aggttcgctt agcgcgtcac tcagaatgga gatatgcgta aaacctgctt
     2701 tttgcagtcg cgttttgacg gcgtcaacat ctttgccatt accgtacagc gccaccgggg
     2761 catcggcttt caggttatgt tgcttgatcc acgcgttgag ctgttcggtg ctcattttgt
     2821 ccagccagct agcagagagg tttaaggcgg caggttcatg accagaaggg ccatttaagg
     2881 tttgtggcca gccgttataa aacgcgctgg ggcgagtatc tatcgctttg ccattttgtt
     2941 gctgaagttg gtcaagtgta agaggcttcg ccagttcagc ggcccacgaa gaagcgcaag
     3001 ccagccctaa agccattgcc agcgcggtca tttgagaaac acgtttcatc gaaaatcccg
     3061 acattgtagt tttgagtgcg ccattctgaa ctgacgcatt gagaaaaact ttgcgttatc
     3121 gcagtttgtt gtaattttct gaccactgcg ccatatccag aacagaacta tcagcaggaa
     3181 catcctggag atcgtgcgtt acctgaacga cggggatcgc cagggcgcga acttcgctga
     3241 acacccactg gcgaaaatta tcgcgcagag ccacatcaag acggctgaat ggctcatcca
     3301 ggagtaacgc ttttggctgg gcgagaaggg cgcgtagtag agcaacgcgc gctcgctgac
     3361 cgccagacaa agtggcagga tcctgatgga aagctccgtc aaggcctgaa cgctcaagtg
     3421 catcattcac ggcattacgt cgggcattcc ccttaagtgt cgccggtagc gccagcagta
     3481 aattttgccc gacgctgaac tggtcaaata acagtgcatc ctgaaaaaga atgccaatct
     3541 gacgctgtgc ggtgggtagc atgtcaatcc gttgctcatt gagccatagc tcacctgtac
     3601 aagaaaactg tccggccagt gcaccaatca tccatgaaaa cagagtggat tttccacagc
     3661 cagacggccc cattaacgtg acgatgtcac ctttatcaac cgtaaagtta acgtttgtca
     3721 gcaagcggct ttctggtaaa cgtagcgaaa catttttcac gcagagcatt agcggattcc
     3781 ttgtctgaca taacctaccc attttgcgac taacgcggtc agggcaaaaa taataagcgg
     3841 taacaacagt tgccataacg cctgggcggc gagaataccg ttgctgccgc cgctgcttaa
     3901 tgccaccgcc tcagtggtga gcgtcggaaa acgccccgcg cccagccaca gcgtcggcat
     3961 atactgcgca atactgactg aaaatcccac cgcgaaggca atcagcacag ggcgcaacat
     4021 gagtgggcat ttcacgtaaa agaagatttt ggcccgcgac cagcccagtg tttgcgcaat
     4081 caaaattaac cgtgaatcaa tgcgctgcca ggcgggttgc aggataaaca gcatccacgg
     4141 cattacccac agcagatgcc cccagaccac cgccgtccag cttccatcca ggttcagcca
     4201 tagcgccagc gtgtactggc ccgccaccag tggcagagcg ggcaatagaa tgggcaacca
     4261 tagccacaac tggcgacgct gtgcccccca ttccagccac agcagtaaca ggagcaaagc
     4321 gatgaatgtt gctaccagcc ccattgtcag actgttgatg agtgcttcac tattgatcgt
     4381 cgactgatcc gcgaggatcg ccagcagaac cacacacagc acaccggtta agggtaaaaa
     4441 actcgccagc gtagtgcctg gtaataaagg tgtggcaggc ttgcgaatgc catctacgcg
     4501 gggaatagtg cgccgccagc tgcgccaaaa cgtaggcggc gagtaacagc atcagcagca
     4561 gactggcgag tgcgcctttc gtttgttgat ccgcgtcgcc ctgagttaac cactgccagc
     4621 taattactgc cagcgtcggt ggattacctg gcccaagaat aatcgccaca tccacgaccg
     4681 aaagcgacca ggcaacaatc gccagcatcg ccattgccag cggctgtagc ccagcgaatc
     4741 cagcacaatg acctgctgca acagccgttt ttcgctcaat actgccacta agatccacag
     4801 cagaaatgcg ctttctttca ctgcaagggt gaggcccaga ccgatccaaa tcggtccatt
     4861 ggcggagtga aatacgggaa atagtcatat agcagccctc cgtcagcaaa gagcagcagg
     4921 gcgctggtgg caaaagccac atggggaatg gcgagcagcc acggcagacg ggcgcacata
     4981 cgctgccatt tcggcccagg ccatagcgcc acaatcacca gcagggcaat caacaatgcc
     5041 ccgaccgccg cgatggttgt cgacaccagc gttgccagta atgcctgcgg taactgtgga
     5101 tcggcaaata acgcctgcca gcgtgtcaac gacaacgcag gcgatatcag cgtgagagct
     5161 gccgggatca gcggtgcata aatcaccgcc accatcgccc acagcaaaaa gattaatgcg
     5221 taccgtaacg gcgtaaccat tcttgttcca gtgcattcac ccatcctgcg tgcggttcag
     5281 ccagtaccgg cggcagatcc tgcggcattc ttgattgcaa tatttcgcgc tgcccgtcag
     5341 gcagtttttg cggatcgaga acagaaggat cgccccagac aacgggatct gctttacgca
     5401 gttgcgcatc gggtgagagc aggaaattgg caactacctt cgccgcagca ctggcattcg
     5461 cgttggctgg aatggtgacg aaatgcacgt tgccaatcat cccctcgcga aagccaaaac
     5521 tgtaactgct tgccggcaaa tcgccgctgg cgattttttg ctgcgcatgt gcggggttaa
     5581 aggtcagcga caggcgcaat gtgccggctt tcagcagagc atccatccgc gcgggcgaag
     5641 gcgggaaatc ctttccttcg cgccacaaat acggatgcag tgcatcaaga tattgctaca
     5701 agggcgcagt gacacgggcg aaagtcgcat cgtccggcgc ttcttttaat gcggcgggat
     5761 ctggcgtcag cataatcagc aactgttcaa gaaacgccgt gccggtaaag tccggtgggc
     5821 gcggataagt aaccgtgccg ggattggctt tagcaaactc cagtaaggct tgcggcgttt
     5881 gtggtggctg tggcgtaaca tcgcggcggg cgataaacgt cagttgtgcg ccgccccagg
     5941 gcgattccgc accttgtgtc ggcactgaaa aatctccccg caccggcagc tgtgtgtcga
     6001 catagcgcca gttgggcaga gtctccgccc agcccgtttg taataaattg gcctctttta
//
LOCUS       DAJTUI010000165         5983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTUI010000165 DAJTUI010000000
VERSION     DAJTUI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.165"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(1..393)
                     /locus_tag="OO464_003839"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="OO464_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO464_003840"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO464_003840"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR6228803.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="OO464_003841"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="OO464_003841"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR6228804.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..>5983
                     /locus_tag="OO464_003842"
     CDS             5797..>5983
                     /locus_tag="OO464_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6228805.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1840 a         1355 c         1018 g         1770 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg taaattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga aactctgcgt
     5941 gtctgggtac gccagcatga gcgggatacc gggggcggtg agg
//
LOCUS       DAJTUI010000166         5829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTUI010000166 DAJTUI010000000
VERSION     DAJTUI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.166"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            79..258
                     /locus_tag="OO464_003843"
                     /pseudo
     CDS             79..258
                     /locus_tag="OO464_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            783..1961
                     /gene="ltrA"
                     /locus_tag="OO464_003844"
     CDS             783..1961
                     /gene="ltrA"
                     /locus_tag="OO464_003844"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6228806.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            2044..2223
                     /locus_tag="OO464_003845"
                     /pseudo
     CDS             2044..2223
                     /locus_tag="OO464_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2253..2735)
                     /locus_tag="OO464_003846"
                     /pseudo
     CDS             complement(2253..2735)
                     /locus_tag="OO464_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2991..3971)
                     /locus_tag="OO464_003847"
     CDS             complement(2991..3971)
                     /locus_tag="OO464_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6228807.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3971..5170)
                     /locus_tag="OO464_003848"
     CDS             complement(3971..5170)
                     /locus_tag="OO464_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6228808.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5492..>5829)
                     /locus_tag="OO464_003849"
     CDS             complement(5492..>5829)
                     /locus_tag="OO464_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6228809.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1597 a         1339 c         1389 g         1504 t
ORIGIN      
        1 gcttattatg cttccatcgg aaacgatgat ctggcagcct gagttcacag ataaaacact
       61 ctccaggaaa cccggggcgg ttcagttgct gaactgctgg aaccgctgta tgacatcctg
      121 cgccagtatg tgctgatgcc cggtaaagtc catgccgatg atatccccgt tccggtccag
      181 gagccgggca gcggtaaaac aaggactgcc cggctgtggg tctacgttcg tgatgaccgt
      241 aacgccggtt cggaaattgc agcataagtt atccatgctc tgaggtgggt agaggtgggc
      301 cctccggtca gggtttacag gagcacagac caaacgaccc ggtgctgctt cattctaagg
      361 aatgaggtgg tgagcgactc gggaaaaggc agagatctac tgtcacgtgg gttcgggaag
      421 ctgaacgaaa gtaaactgtc cgatgaagca tcgaaatctt agatgatgcc aaaatggggc
      481 cgtacagccg tcccctgata agcgcagaag acacctgctt actgactgcg cggcatccgg
      541 tgtgaaggca gcaggatccc gggccagacc atctcaggga acgtgagaac ctgtttccgg
      601 cacgtatcgg gaaacagggg cggacccatt cgtagtagtg atgaaagatc tgtaatgggt
      661 ctggagcgaa gggatgggga tcacattcat tgcagaagac acaacccaaa aggggatgac
      721 gtaactgatg caacgaaaat catttgaaat accaaaagca ctggtctggg catcatatct
      781 ggatgtgcgt cgcaacaagg gagccccccg gatgcgacgg gcagactttg aaaatgttcg
      841 accagcaacg tgatggcaat ctatacaaga tctggaaccg gctatgttcg ggaacatggt
      901 ttccgccacc agtgctggaa aaacggatcc cgaagtcgaa tggcaaggaa cgcattctgg
      961 ggatcccaac agtatccgat cgaattgctc agggagcgat aaaacttttc atggaagaaa
     1021 agcttgaccc gattttccat gctgattcat atggttaccg accaggcaaa tcagctcatg
     1081 acgctctgaa acaatgtgcc atccggtgct ggcgttatag ctggatactg gaagtggaca
     1141 tcagtgcgtt tttcgaccat gtgagacatg atctggtgct caaagctctg gaacaccacg
     1201 ggatgcctaa atgggtcatc ctgtactgtc gacgctggat ggaagcgcca atgcagagtt
     1261 gtgaaaatgg agaattaata acccgaacac gaggcactcc gcagggcggg gtcattagtc
     1321 cgctacttgc taacttgttc cttcactatg catttgattt gtggatggaa agagaatatc
     1381 ggggggtacc gtttgagagg tacgctgacg atattgtagt acattgttca cgaatgagtg
     1441 atgcaacaag gctaaagaac aggttatcgg agcgcttctc ggaagtcggg ctggtcctga
     1501 atgcagggaa aacgaatatc gcctacattg acacgtttaa aaggagaaac gtcgcaacga
     1561 gtttcacctt tctcggatat gacttcaaag tgcgtacgct gaagaatttc aacggcgaac
     1621 ggtaccgaaa atgcatgccg ggtgcgtcaa atgcagcaat gcgcaaaata acagaaacaa
     1681 tcaagaagtg gcgtatacat cgctcaacag ctgagagttt gctggatttt gcgaggcgct
     1741 acaatgcgat agtgagaggc tggatcgagt actacggaaa gttctggtcc agaaatttca
     1801 actatcgact gtggagtgca atgcagtcac gtctgctcaa gtggatgcag tctaaataca
     1861 gactttcgaa ccggaaggct cagcgaaagc tgacgctggt aaggaaggag tatccgaagc
     1921 tatttgttca ctggtattta ctacgtgcat caaatgagtg atcaagagcc gtatgacgcg
     1981 agagtgtcac gtacggttcc gtgagcagcc ggggagtgaa attctctccg gctgactcga
     2041 ctcgcgggtt ccgacagcga tgacgaacat gcggcggtgt tgtactcgct gatcggcaca
     2101 tgccgtctga acaatgtgga accagaaaaa tggctgcgtt acgtcattga gcatatccag
     2161 gactggccgg caaaccgggt acgtgatctg ttgccctgga aagttgatct gagctctcag
     2221 taaatatcaa tacggttctg acgagccgct tacttttggc gtaattctgc tgcgccttta
     2281 ctgaacacaa tgccgtattc agcaagaagt ccacgaatgt gatttatcag ttgtgtcctt
     2341 tccattgaaa ccaaccgacg catattcaaa aaagtctgga cgtcctgttg ctccagagtt
     2401 tttattggta cgggtcgtat agttccatgt tggcaggcct cagctattgc ctgtgcgtcg
     2461 ttataatcat ttttctggca ccgctgataa gctttaacat gttgtgccgg aagcaatatt
     2521 acatcatggt cgagtttcct gatttcccgg gcccagtaat gagatgcccc gccagcctcc
     2581 atcgcaacag tcattttagg ctgttgcacc agccagttaa gcagttgatt gcgagaaagt
     2641 ttggtctttc ctgcgggttt gccatgagca ttgatactga aaagataaaa gttcgtttta
     2701 gccagatcaa tgccggtgat tttctgttca gacatggtat cccgcttact tttgttgtta
     2761 aagtttatcc atcatggcgc aattgaaggc tgccgtgaaa gagggggaag actatctcat
     2821 cggttactgt taaacggcta agagaaacta tctttttcaa gcaaagaaga tacggtgaaa
     2881 caggcgattt caccgtgaaa tactgttcaa aaaaaacgct aataaaagca aaatttcttt
     2941 taaaacagcc aattaaaaac tggcgagttg ctggcgaaaa acttgaattt tcacagcgaa
     3001 ttcagattat cttttagtac aagagcaatc atacgatcta acttatcctg aacttccaac
     3061 ggtaatctgc cgaattcata tgagaacgta cgtcccttta ctcgttttcg agcaaattta
     3121 tctttactat caaatttcca taacaaagtt acaaccgctt tatcctttac tcctgaatca
     3181 agcaatgaag acatttcctt ggttataagc cgcataatac tgtttttaac ctcgtcttca
     3241 gaaagattgt catcagcagt aataagctca atactcggcg agatattctt cacaacttca
     3301 tcaatagata tatcacccag cctcagtcgt tcacttaatt cagccagttg cctgtaatcg
     3361 gcgtatgtca gctcagactg aacaggaaaa agagacacaa aatctttagg tacggacgct
     3421 gcttggaggg cacgagtcac cttagctgcc gacaatccct ctctttccgc aatttgcttc
     3481 tggttcatac cggcctcttt gagacgtact aaacgcaacc caatttcacg aatattatgt
     3541 tcaagtgacg tctgcaaatc tttcgccaga tgctgagctt cagaaacagt aagctcatca
     3601 tccgttacaa gtacacgtaa tccaaccttg catagcaaag ctgctgcccg acgacgggaa
     3661 ccatccaaaa tctcaataag atcgccagtc cgaatcccaa tacatggata gaattgttgt
     3721 aaacgtatag ttctggtgat gtcttttagt gagtccggcg tcagggctgt ctgttcgcgg
     3781 ccattcacct cttcaaccac aaaagtttga gtatccactt tgtcatgatc aaccttgatc
     3841 tcagtaaatt ttgcctttcg gccagtattg agggtaaata catgaactgg agccgatatt
     3901 tcttcagtat tattcaggat atttgtattc agcgtacggc caatagtcgg tcggtgcttt
     3961 ctgttttcca tcagtagttg gcccttataa attcaatacg atcaaaaact gctttagcga
     4021 aatcttctgc tgccatacgt gcatttttca aagcttcacc actgccaaca taagtaacag
     4081 gattcgcaga tatcacggta tcaaacgact cgccgcttct ctcaaaacca tcaagacgtg
     4141 gcattgaaac atcaagcatg tcaccaccga aaatctcttt tgtcaggcta tggcagtatt
     4201 tatgatctga cttattcgcc agtttcgaca tgaagccaat atttgcctgc aaacggcatg
     4261 aacatccatc ctgttcaata atctgtacaa gttcaggtaa acgagccaaa tatttaagcg
     4321 tggaatggaa atcaacttgt gctggcggca ccggtgtaaa catgatatct gcggcagcaa
     4381 tggcattttt caaaaatgcg tccagatgcg ggcctgtatc aataaggatg aagtcgaaat
     4441 catgcttcag cttatcaata atattctcac gcaatatcgc gtgtttgttt tgtcctaata
     4501 gatgctcctc gcacagagta tcccagtttg atgcaataaa ggcgtcgtca atagaagccg
     4561 gcataacata aacgccaggg ataactgacg gaacgataaa atcttccagt aattcctcac
     4621 gactcacgtt ctgcaacata gcctgcggtg cggttgtatc aaccagcccc accgcatgca
     4681 agtagttcag aaacatagtg gctgatgact ggggatctaa atcaaggagc agaatacgta
     4741 aatcttctga cagcaggtga ggatgagcac gcagggcgtg agctacggaa acactactca
     4801 cggttttaga cacaccgcct tttagactac cgatgaatat ggaatatgcc tcactataac
     4861 gatcgcgata tttaggaatg ccacgatgtg catagatatc gataatgttc tggatggtta
     4921 atgcatattt tgtcgcggtt cctgctgggc gtttttcaaa ctgatagccc tgagcctcca
     4981 tctcaccgac tgcaccatcc acgcttgcgc gcgttaattt tggaagctta gctacagcag
     5041 actttgaata gacttgataa aaaacatcag cctgaaactc ctgcttttgc tcctgaatct
     5101 gttttgttag ggcgagcaac attttatctg cacgctgcgc aaccttactt aactgctcaa
     5161 aactagtcat tccccctccg tgctatgtag gattttaact tattacaaaa aaaactgcat
     5221 tagcagggta atgcatgcaa gtacatattg tacatgctta tattaaaaat gcgcgcattg
     5281 ctgatttatt aatcatcgga atgaatatta ctgtaagata gtatgccttt ccgggagaaa
     5341 gacgctgatt tcccccgaat aatccagttc accaccacac tccggacaac agctttcttc
     5401 aggctccaga tggtgtattt cgcgggaaac atgttccggt aacggacggc gatgacgtga
     5461 ctgtcgcaac aggcgggggt agtgcttcca atcagtagaa catgtgcttt tcaatgaagg
     5521 agccgatgac tttttcgtgg atttccacgg aacgcgagaa gcagattgtt ttacgtgcca
     5581 gccgcttgat gcgggtgcgg agggtccggt tgttacgtgc aatacgttgc gtgaagattt
     5641 tgccggtcag gtgtttctcc ttcggcactt ctctggcgta gctgccccag tcatcgctgg
     5701 tcaccatgcc gatacagaac ggagtcaaca gggctaacag ttcccgacag gtctcatcat
     5761 tccgtggccc gaaagtgtaa gccaacacac cgccagtttt agtgttgtac gcataccaga
     5821 gccagtgtt
//
LOCUS       DAJTUI010000167         5795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTUI010000167 DAJTUI010000000
VERSION     DAJTUI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.167"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            53..571
                     /gene="mddA"
                     /locus_tag="OO464_003850"
     CDS             53..571
                     /gene="mddA"
                     /locus_tag="OO464_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR6228810.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            568..1017
                     /locus_tag="OO464_003851"
     CDS             568..1017
                     /locus_tag="OO464_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6228811.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1018..1251)
                     /locus_tag="OO464_003852"
     CDS             complement(1018..1251)
                     /locus_tag="OO464_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR6228812.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1337..1510)
                     /gene="ortT"
                     /locus_tag="OO464_003853"
     CDS             complement(1337..1510)
                     /gene="ortT"
                     /locus_tag="OO464_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR6228813.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1705..1800
                     /gene="yncL"
                     /locus_tag="OO464_003854"
     CDS             1705..1800
                     /gene="yncL"
                     /locus_tag="OO464_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR6228814.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2202..3011)
                     /locus_tag="OO464_003855"
     CDS             complement(2202..3011)
                     /locus_tag="OO464_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR6228815.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3221..4645)
                     /gene="patD"
                     /locus_tag="OO464_003856"
     CDS             complement(3221..4645)
                     /gene="patD"
                     /locus_tag="OO464_003856"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR6228816.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4667..5461)
                     /locus_tag="OO464_003857"
     CDS             complement(4667..5461)
                     /locus_tag="OO464_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6228817.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5451..>5795)
                     /locus_tag="OO464_003858"
     CDS             complement(5451..>5795)
                     /locus_tag="OO464_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6228818.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1522 a         1471 c         1283 g         1519 t
ORIGIN      
        1 tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag acatgtccat
       61 ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc acgccgtgtt
      121 gtatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca ttgcctggtt
      181 tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg gcgtagttac
      241 gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata ccgtggaaca
      301 ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat tgttaagtcg
      361 attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga tcgaatcgca
      421 aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg cacaaatgcc
      481 gcaggtaggc actaaatttg gtcattggct ggatctgaca tttatgcagt tgcaactcga
      541 cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc ttttttgatt
      601 gccgccggaa ttggtctggt agtccagaac acgctgatgg cgcgcatcac ccagacctcc
      661 tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct ctttgtttct
      721 attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag cgtgcgctgg
      781 tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat cagcggatat
      841 cagaatgtgg gagcggcgac aactattgcg gtactggtcg ccagccagct gattggcggt
      901 ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt cggcccaata
      961 tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc attttaatca
     1021 gaggatggtg ccgcctttgg ttaactgttc gtggcgcgct tccatatctt ctttatgctt
     1081 gcgaccgtga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc gatcttcccg
     1141 acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg caatgacgct
     1201 ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca tactttctcc
     1261 ttagccactt gtgaaattta tcgattaatc caaatgatat aagtctgcgt ggtgccgtca
     1321 agccagtgag taagatttaa aacagtgaaa acaacagcgc caccgggaaa ctcatcggcc
     1381 atgtggcccc caccagaaag gcgcttaaga aacggatgcg tttcttatcg tgagaaagaa
     1441 accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc atatgttgat
     1501 agagaaacat gtacgggcgt ccagccatat gaataaaacg cgcgcaaata tgcgccattt
     1561 tttgacgtgc atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca gcaacttgcc
     1621 cttatccatc ttcagaatga gtattattgc ggattttctt aacccgtact atacacatag
     1681 gggcaatgat aaaaggtggc aaaaatgaat gtttccagta gaactgtagt actgataaat
     1741 gtctttgctg ctgttggttt gtttactctt atctctatga gatttggctg gtttatttga
     1801 tgtagtgatt ttgcacgcag cacgtactgt cagagaaatc aactctctga cagtacgcaa
     1861 gatcttctca tactctccaa gtttgcaaca ttatccttaa tatttacttc tttttgctgg
     1921 catctacatt gaaatgagta gaaaacatcc ccctactgtc gtctatacaa acagcttact
     1981 gattcaaacg gaatcttgtt ttcacatttt tctatcacga atactttata cgcgcgggag
     2041 tctataaatg ctttatactt tcaaggaata acgtaaatat aaaaagatgg cgtaaagtat
     2101 atgcatggca ggatgtttgc aacctccttg atgacgctgc cccattgaaa ttgcaaaccc
     2161 cgccctggtt atcttgccca tttatcatgc aaaacagata attataggcc tctgaatacc
     2221 tcagaaagat tgaatttttt atagtccctc tcttgaatat ttccatttga tatcaatcga
     2281 ttcatctctt caacaaaata atttatatca tcacacttat tattatccgg aagatctgag
     2341 tagttataat tccaccattt tattctcaac agttcatcaa taacatttga atcaaaccta
     2401 aatctgatta tttttgcagg aactcctgca actatggcat atgggggcac atctttggta
     2461 acaacggaat tggcagcaat aacagcacca tcacctattg cgatgccacc tttcaataca
     2521 acatcatttc ctatccagac atcatttcct ataatcggat tttcaggttt ggtaatgaat
     2581 ggtttcaatg tccagctacg ttcaaattca ctcatagcaa acttatcaaa ttcaccgtta
     2641 taagtcatca tgtgagtagt aaaacgattc aaaggatgat ttccacccat tatttttaca
     2701 tttgatgcaa tactacaaaa tcgacctata cgtatattcc ctgaaaaatg gcaggtcgga
     2761 aatgaaaaag cgccgacact ggagaaattt ttcacaggca ttgttgaata ttcttcagca
     2821 ataacattat cttcaattgt tatcttgtca ccttttgaga aaagaaataa attcctttct
     2881 ttatacgact tcttcccctt atgatttaaa aagattttat tatccataca aaactcaaag
     2941 tgttttttcg tccatctgaa ggtaagggtt ttatttttat cttccgtgaa tttgtacaat
     3001 tgaactttca ttgttaatac ttctacttat aaagtattag tttaataacg atactctaaa
     3061 gccttatgct aatcaactgt gacaggatga aaaatcgcca tcaaacattt agcctaattt
     3121 ttacgccagt tattaatgta atcattctaa tacttaaccg ccattaaact cagaacctaa
     3181 aacgatagtg gccgtcctat aacagccact aacatctaac ttaatgttta accatgacgt
     3241 ggcggacgac ggtgtaatcc tccagcccat aaagtgacat atccttgccg taaccagaaa
     3301 gtttctgccc accgtgcggc atttcactta ccagcatgaa atgggtattg acccaggtac
     3361 aaccatattg cagccgtgcg ctgacgcgat gcgccctgcc cacatctttc gtccataccg
     3421 aagatgcaag tccgtactgg ctgtcattcg cccagttcac cacctgttct tcgttgtcga
     3481 agggcgtaac actcactact ggaccaaata cctctttttg cacgatggca tcgtcctgta
     3541 atgcgccagc cagcagcgtc ggcgcatagt aatagccatt acccttgcgc ttttcaccgc
     3601 cagtgatcac tttgatgtgc cctgtcgctt tcgcctcttc tactgccttg ctgacgcgtt
     3661 cgagatgcgc cagcgagctt aaaggtccaa gctccgtaga ctcgtcatcc ggcgcaccag
     3721 attttaacgt tgccaccgca gcacccagtt tttccaccag cgtatcgtaa atgccttttt
     3781 gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag ccaaatgtac
     3841 gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact ggcgctttgc
     3901 caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg atgtgctcgc
     3961 cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg gtcagcggat
     4021 cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg aagatatctt
     4081 tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt ttaagcacta
     4141 cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc agcggataat
     4201 tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc gaagtatgac
     4261 cttcaagata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc gcgaaaaagc
     4321 gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc ggtttgccac
     4381 aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca tccgccagtt
     4441 tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca aatgctgcat
     4501 ctgccgcacg cacagcagca tcgacctgct ctgcggatac ctcggcaatc tccagtaaaa
     4561 cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta acccgttctc
     4621 cgttaatcag taacttatgt tgcatagctt attcctgtat cagtggttat tgaccattgt
     4681 cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc aacataacca
     4741 gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga ttgagcaacc
     4801 acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt tcatcgaacg
     4861 acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc ggcaacacta
     4921 cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca accagactcc
     4981 acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg caaaaagtcg
     5041 catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta aaggcggtta
     5101 atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat aacagcgaaa
     5161 tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc gtccctaaca
     5221 ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg gcatcaagaa
     5281 tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc ggtggaaaac
     5341 taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg ggaaaatgta
     5401 ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt tcagagtgca
     5461 tcgaacgctc ccagacgttt cacgaacgcc aggtacagtg cgatgagaat aatcggcacc
     5521 agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga ataaaccata
     5581 ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc gcccagtgtg
     5641 agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg cagcaccaca
     5701 tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa caatgacggc
     5761 ggcaaacgct caagcgccgc ctgaacgggc aggat
//
LOCUS       DAJTUI010000168         5737 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTUI010000168 DAJTUI010000000
VERSION     DAJTUI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.168"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            5..343
                     /gene="spa13"
                     /locus_tag="OO464_003859"
                     /gene_synonym="spaM"
     CDS             5..343
                     /gene="spa13"
                     /locus_tag="OO464_003859"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR6228819.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            330..1208
                     /gene="spa32"
                     /locus_tag="OO464_003860"
     CDS             330..1208
                     /gene="spa32"
                     /locus_tag="OO464_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR6228820.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            1202..2083
                     /gene="spa33"
                     /locus_tag="OO464_003861"
                     /gene_synonym="spaO"
     CDS             1202..2083
                     /gene="spa33"
                     /locus_tag="OO464_003861"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR6228821.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            2088..2738
                     /gene="spa24"
                     /locus_tag="OO464_003862"
                     /gene_synonym="spaP"
     CDS             2088..2738
                     /gene="spa24"
                     /locus_tag="OO464_003862"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR6228822.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            2753..3013
                     /gene="spaQ"
                     /locus_tag="OO464_003863"
     CDS             2753..3013
                     /gene="spaQ"
                     /locus_tag="OO464_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR6228823.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            3013..3783
                     /gene="spa29"
                     /locus_tag="OO464_003864"
                     /gene_synonym="spaR"
     CDS             3013..3783
                     /gene="spa29"
                     /locus_tag="OO464_003864"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR6228824.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            3790..4818
                     /gene="spa40"
                     /locus_tag="OO464_003865"
                     /gene_synonym="spaS"
     CDS             3790..4818
                     /gene="spa40"
                     /locus_tag="OO464_003865"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR6228825.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            4831..5073
                     /locus_tag="OO464_003866"
     CDS             4831..5073
                     /locus_tag="OO464_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228826.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT         1823 a          824 c         1058 g         2032 t
ORIGIN      
        1 agatgtggag gcattagata aaaggattat atacttcctg caactggaga atgacttaga
       61 acctgtaggc gctcagagtg ttagtcagct tttcaataca agacgtaaga tcgctatagt
      121 aaaaaagcat attatccaat atcagtctga acgaattctt ctaaagggaa gaattgaaga
      181 aattcaaaaa gatattgatg aggcaaatgc ttctaaaaga aaactgcttc ataaagagag
      241 taaaatatgt aaacggattg gcttgattaa aagaaataac tttgccaaac agttgatact
      301 tgatgagtta tcacaagagg atatgaagta tggcattaga taatataaac ctaaatttta
      361 gtagtgacaa acagatagaa aaatgtgaga aactatcttc aatagataat attgatagtc
      421 tcgttttgaa gaagaagagg aaggttgaaa ttcctgagta ctctttaatt gcatctaact
      481 attttactat tgataagcac tttgaacata agcatgataa aggagaaatt tatagtggca
      541 ttaaaaatgc gttcgaactt agaaacgaac gagcgacata ttctgatatt ccagaatcaa
      601 tggccattaa agaaaatatt ttgataccag atcaagatat caaagcaagg gaaaaaataa
      661 atatcggcga tatgaggggg atcttttcat ataataagag tggaaatgca gacaagaact
      721 tcgaaagaag tcatacttct tctgtaaacc ctgataatct gctagaatct gataatagaa
      781 atggtcaaat tggtttaaaa aatcatagct tgtctattga taagaatatt gctgacatca
      841 tttctttact aaatggaagt gttgctaaat catttgagct gcctgtaatg aataaaaata
      901 ctgcagacat aaccccatcc atgtcattgc aagaaaaatc aatagttgaa aatgataaaa
      961 atgtttttca aaaaaatagt gaaatgactt accactttaa acagtggggg gctggacatt
     1021 ctgttagtat ttcagtggag tctggttctt ttgttctaaa accgtcagat caatttgtag
     1081 gaaataaact tgacttaatt ttgaaacaag atgctgaggg taattacaga tttgatagca
     1141 gtcaacataa taaggggaat aaaaataata gtacaggata taatgaacag agtgaagaag
     1201 aatgctaaga attaaacatt ttgacgctaa cgaaaaacta cagattttat atgcaaagca
     1261 actctgcgag cgtttttcga ttcagacatt caaaaataaa tttacaggca gtgaaagttt
     1321 agtcactctt acttccgtgt gtggggattg ggtaattcgt attgatacat tatctttttt
     1381 gaaaaaaaaa tacgaggtat tttcaggatt ttctacacaa gaatctttac tgcatttatc
     1441 aaaatgtgtc tttatagagt cgtcatctgt attttcgatt ccagaactgt ctgataagat
     1501 tactttccgg atcacgaatg aaatccagta tgcaactact gggagtcatt tatgctgttt
     1561 ttcctcttct ttaggtatta tttattttga caagatgccg gtattacgta atcaagtttc
     1621 tcttgactca ttgcatcatc ttttagagtt ttgcttaggt tcatctaatg taaggctggc
     1681 tactttaaaa agaattcgca ctggtgatat aatcatagtt cagaaacttt ataatttatt
     1741 attgtgtaat caagttatta ttggggatta tattgtgaat gataataatg aggcaaaaat
     1801 taatctgtca gaaagtaatg gtgagtcaga acacacagaa gtttctttgg cattattcaa
     1861 ttatgatgat atcaatgtaa aagtggactt tattctttta gaaaaaaata tgacaatcaa
     1921 tgaactaaaa atgtatgtag aaaacgaatt attcaagttt cccgatgaca tagttaaaca
     1981 tgtaaatatt aaagtaaatg gttctttggt tgggcatggg gaacttgttt ctattgagga
     2041 tggttatggt atcgagatta gttcttggat ggtaaaggag taatctcatg ctgagtgaca
     2101 tgtccctcat cgctacactt tcttttttta cgctattacc atttttagtt gctgctggta
     2161 cctgttatat aaagttttct attgtttttg taatggtacg aaatgcactt ggtttgcagc
     2221 aggttccatc aaatatgact ctcaatggta ttgcattgat tatggctttg ttcgtgatga
     2281 aacccattat tgaagctggt tatgaaaatt atctaaatgg ccctcaaaaa tttgatacaa
     2341 tatctgatat tgtccgtttt tctgattctg gcttgatgga atataagcaa tacttgaaaa
     2401 agcatacaga tcttgagtta gcacgttttt tccaaaggtc tgaagaagaa aatgctgatt
     2461 taaagagtgc agagaataat gattattcat tgttttccct cttgcctgca tatgcgctaa
     2521 gtgaaatcaa agatgcgttc aaaataggtt tttatttata tttgccattt gttgttgttg
     2581 atcttgttat ttcaagcatt ttacttgcac ttggtatgat gatgatgagt ccaatcacaa
     2641 tatcagtacc aataaaactt gtattatttg ttgcgcttga tgggtggggt atcctgtcta
     2701 aggccttgat tgaacaatat atcaatattc ctgcttagat gatggaggta atgtgtctga
     2761 tatagtttat atgggtaata aggctcttta tttaatcctt atcttttcct tatggccagt
     2821 aggtatagct acggttattg gattatctat tggtttgtta cagacagtga ctcaactcca
     2881 agagcagaca cttccttttg gtataaagct tataggtgtc tcaatatctt tgctacttct
     2941 ttctggatgg tatggtgagg ttttattgtc tttttgtcat gaaataatgt ttttaattaa
     3001 gagtggggtt tgatggacat ttcaagctgg ttcgaaagta ttcatgtgtt tttaatactc
     3061 ctgaacggcg ttttttttag attggctcca ttatttttct ttcttccatt tttaaataac
     3121 ggtataattt ctccatctat tagaatacct gtgatttttc ttgttgcatc aggattaatt
     3181 acttctggta aggtagacat aggttcttct gtttttgaac atgtttattt ccttatgttc
     3241 aaggaaataa ttgttggcct ccttctctct ttttgcttgt ctcttccctt ttggatattt
     3301 catgctgttg gtagcattat tgacaaccag cgtggggcaa cgcttagtag ttcaattgat
     3361 cctgccaatg gtgttgatac gtctgagttg gcaaaatttt tcaatctttt ttctgcagtt
     3421 gtatttctat acagtggtgg tatggtcttt attttagaat ccatacaatt gtcttataat
     3481 atatgcccgt tattttctca atgttctttc cgtgtctcaa atatcttaac atttctgact
     3541 ttattggcaa gtcaggctgt tattttagcc agtcctgtta tgatagtatt gttactatca
     3601 gaagtattac ttggtgtatt atcgagattt gctccgcaga tgaatgcttt ttccgtatca
     3661 ttaactatta aaagtttact tgcaatattt attattttca tctgttcttc tactatttac
     3721 ttttctaaag ttcaattttt cctcggtgaa cataaatttt tcacaaatct atttgttaga
     3781 taaaatatta tggcaaataa aacagaaaag ccgacaccta aaaaactaaa ggatgccgca
     3841 aaaaaaggac agtcatttaa atttaaggat ttaacgactg ttgttattat tctggtaggg
     3901 acatttacta taatatcatt cttttcctta agtgatgtaa tgctcttata cagatatgta
     3961 ataattaatg acttcgaaat taatgagggt aaatactttt ttgcagtggt tattgtcttt
     4021 tttaagataa ttggcttccc actttttttc tgtgttcttt cggctgtgtt gccaacattg
     4081 gttcaaacaa agtttgttct tgcgactaaa gctatcaaga ttgatttttc agtattaaac
     4141 cctgttaaag ggttaaaaaa aatatttagt ataaagacaa taaaagaatt tttcaaaagc
     4201 attctgcttc ttattattct agcattaaca acctatttct tttggattaa tgaccgaaaa
     4261 ataatttttt ctcaggtgtt ttctagtgtt gatggcttat atcttatttg ggggaggctg
     4321 tttaaggata taatattatt tttcttggca ttttctattc ttgttattat tcttgacttt
     4381 gtgattgagt tcattttata catgaaagat atgatgatgg ataaacagga gataaaaaga
     4441 gaatatatag agcaagaggg acactttgag acaaagtcga gaaggcgtga gttgcatatc
     4501 gagattcttt cagagcagac taaatctgat atacgtaatt caaaattagt ggtaatgaac
     4561 ccgactcata ttgcaattgg tatttatttt aatccagaaa tagcgcctgc accttttatt
     4621 tctctcattg aaactaacca gtgtgccttg gctgtcagaa aatatgcaaa tgaagttggt
     4681 ataccgactg tgcgtgatgt gaaattagct agaaaactat ataaaacaca tacaaaatat
     4741 agttttgttg attttgaaca cttggatgaa gtcctacgtc ttattgtttg gcttgagcag
     4801 gttgaaaaca ctcattaata agtaaaggag atgtgttata tgggagttaa tttctgtaat
     4861 aaaataggta ttgatcagtc tgaatttgaa atagaatctt ccatcattaa ctccattgct
     4921 aatgaggtat tgaacccaat atctttcctt agcaataagg atataataaa tgttttactc
     4981 agaaagattt catctgaatg tgacctggtt agaaaagaca tttatcgttg tgctctggag
     5041 ttggtcgttg aaaaaacacc tgatgattta taatcattca taataactct tttgctgata
     5101 taaatatttt tgttgaagat atttatgtag tcacaggatc aattgtagaa aattgttatg
     5161 tttttgttag gaactgcact aaataatata tgataagaca gcaaaaacga ctgaccataa
     5221 tccttctttt attaggagtt gataagagag actattcatc ctgtaatgtt aaaactctgt
     5281 tatatagtat tcgcgattat gcaaagtctg taaatgatca tgagatacta accgagagta
     5341 atcgtttatt atctcattgc atttcagata gtaacggagc tttctttaaa agtagtaaat
     5401 acgttcccct taagtactta cgtaaaagga gaatagcacg aaagatacca aatgattagc
     5461 actttgctag taagcttgct ggtaaactat attgaccgtt gggtttgttt tgagactgta
     5521 atttaaattg tgtaattgcc tgtttttgat atgtttactc caacaacgga gacaggtaaa
     5581 ttatggacga aaagaagctt aaagcccttg cggctgaact ggctaaaggc ctcaaaactg
     5641 aggccgacct caatcagttt tcccgtatgc tgacgaaata tcccggctct taaaacgata
     5701 gtggctcagg ctttcatttt tcagcgttcc ccagaag
//
LOCUS       DAJTUI010000169         5670 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTUI010000169 DAJTUI010000000
VERSION     DAJTUI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.169"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            420..1328
                     /locus_tag="OO464_003867"
     CDS             420..1328
                     /locus_tag="OO464_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR6228827.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1374..3365)
                     /gene="tkt"
                     /locus_tag="OO464_003868"
     CDS             complement(1374..3365)
                     /gene="tkt"
                     /locus_tag="OO464_003868"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6228828.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3643..4401
                     /gene="loiP"
                     /locus_tag="OO464_003869"
                     /gene_synonym="yggG"
     CDS             3643..4401
                     /gene="loiP"
                     /locus_tag="OO464_003869"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6228829.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4607..5527)
                     /gene="speB"
                     /locus_tag="OO464_003870"
     CDS             complement(4607..5527)
                     /gene="speB"
                     /locus_tag="OO464_003870"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6228830.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
BASE COUNT         1399 a         1479 c         1467 g         1325 t
ORIGIN      
        1 aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc cttattttat
       61 ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg aaagcaattg
      121 ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata acaacaatat
      181 gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa atcaacattt
      241 tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt cgaaaacata
      301 cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt ctcacctgtc
      361 ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg aagattgtca
      421 tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct atcgccattg
      481 gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat gaaggtgaac
      541 gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg ctgggtttta
      601 agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct gtggtctatc
      661 agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta tcagtcagct
      721 tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact attgaagcat
      781 taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt ttcggccaat
      841 ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg caaaatgcca
      901 tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa aatattgatt
      961 tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc gccattgcca
     1021 cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta actcaggccc
     1081 aggcagaagc agacagtaaa ctggcggcag ccaaagcaga agcggaaact attcgcgtaa
     1141 gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca gaagcaatcc
     1201 gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc actgccgaac
     1261 gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg tttatctcta
     1321 ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac tgattacagc
     1381 agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc aaacagcagc
     1441 tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt caggccaaca
     1501 tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt aaccgctttc
     1561 ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt agacggcatg
     1621 gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc aaccgccagt
     1681 tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca gtctttcagc
     1741 acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg ctgcgccagg
     1801 ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc gtatttccac
     1861 gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg ggtcacgcgc
     1921 agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag accgatggag
     1981 tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg tacggcgtta
     2041 cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc accgtgcagg
     2101 gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg gatgtagtta
     2161 cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt agacggtgcc
     2221 aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc gatagcattc
     2281 tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc gataaactct
     2341 ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt aaattcagcc
     2401 gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc agattctttc
     2461 gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat ttcgaacggt
     2521 gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc cagcggcgca
     2581 ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat ggttttgcac
     2641 atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg tttgatggat
     2701 gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc gaaacgcatt
     2761 gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc gttgtcatcg
     2821 tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac ttcgtgggag
     2881 atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc gacaatgtcg
     2941 tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat accgactgcg
     3001 ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt ataacctact
     3061 tccggatggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc ttccatcggc
     3121 agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg gccgttggac
     3181 agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt caggaaatca
     3241 cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg accggatttg
     3301 gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc tttacgtgag
     3361 gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg acgacagcgc
     3421 gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg tcatgtacat
     3481 ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg cgcaatctgc
     3541 tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac ctgcaaacgg
     3601 cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat tcgcgcctta
     3661 ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga ctccaacgga
     3721 ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc gcaggtgaaa
     3781 accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc gccagccaat
     3841 agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa tatcaacggt
     3901 cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc aatggctaac
     3961 ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga agtcgaagcg
     4021 gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg gatgcaggtg
     4081 gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat tgtcggcagt
     4141 ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca attctcccag
     4201 cgccaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg cggcatcagc
     4261 ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg tcgtcaaagc
     4321 tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg cgatcgcatc
     4381 agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat aaggcgttca
     4441 cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg atgcgatgct
     4501 ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt tcacgccgca
     4561 tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac tcgccctttt
     4621 tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga gcggtgattt
     4681 ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg ttgagatctt
     4741 tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc actggcgtac
     4801 cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg taaaccggca
     4861 tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga tcgttcacct
     4921 ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga ataccaatct
     4981 gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac atagtgccgt
     5041 ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt accagcgcca
     5101 ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag tggtcaccac
     5161 cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc tgcagctttt
     5221 cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc acgacgttca
     5281 gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc agattcgtcg
     5341 aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa gtggccatat
     5401 cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg aagttcatcg
     5461 gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga tgacctaagg
     5521 tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg cgtcgttagg
     5581 cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc gccttaaatg
     5641 ggcgaggcat caagcgaggt tgctatttcc
//
LOCUS       DAJTUI010000170         5601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTUI010000170 DAJTUI010000000
VERSION     DAJTUI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.170"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..411
                     /locus_tag="OO464_003871"
     CDS             <1..411
                     /locus_tag="OO464_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6228831.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="OO464_003872"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="OO464_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OO464_003873"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OO464_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR6228832.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1402 c         1333 g         1488 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc g
//
LOCUS       DAJTUI010000171         5548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTUI010000171 DAJTUI010000000
VERSION     DAJTUI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.171"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..642
                     /locus_tag="OO464_003874"
     CDS             <1..642
                     /locus_tag="OO464_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR6228833.1"
                     /translation="VVGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAIT
                     TGKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQN
                     AVLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCA
                     LGLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(696..1301)
                     /gene="azoR"
                     /locus_tag="OO464_003875"
     CDS             complement(696..1301)
                     /gene="azoR"
                     /locus_tag="OO464_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6228834.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1502..5404
                     /gene="hrpA"
                     /locus_tag="OO464_003876"
     CDS             1502..5404
                     /gene="hrpA"
                     /locus_tag="OO464_003876"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6228835.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1374 a         1357 c         1540 g         1277 t
ORIGIN      
        1 gtcgtaggcg cgtgcattgt gttgatggag ctaatgatga tggttcagtt atggtggtca
       61 gtctggttat gttggccagt attatcgcta ctcattattg gccgtgggtg cggtgggctt
      121 ggcgcgataa caacagggaa agatagtcag gggaaactcc cgcccgccgt ttactggctg
      181 acattgccct ggcgcatcgg gatgtggcta tctatgcgtt ggttttgtcg tcgcctggag
      241 ccggtgagca aaatgactgc tggtgtttat ttaagggcgt ttccacgaca tattccggca
      301 cagaatgcgg ttctggacgt cacctttgaa ttccctcgtg gacgagcgac aaaagatcga
      361 ctctatttct gtgtaccgat gttggatctg gtggttccgg aagaggggga gctccgacag
      421 gccgtggcga tgctggaaac attacgcgaa gagcaaggca gcgttctggt ccattgcgca
      481 ttgggattat cgcgcagtgc gctggtagtg gcggcatggc tgttatgtta cggacactgt
      541 aaaacagttg atgaagcgat tatctttatt cgagccagac gctcgcatat tgtgcttaag
      601 gaagagcaca aagcgatgtt gaaattatgg gaaaacaggt aagtggattg agatgtggac
      661 tgaatatcta cagtccacat caagaccgtg tccggttatg cagcaacaat gctgtcgatg
      721 gctgcttttg cgtcagactg tgctttcgct gccatttccg gaccgtatgc gatcccttcg
      781 gcgaagacaa atttcacatc ggtaatgccg ataaagccga ggaacgtgga cagatacggc
      841 gtcaccaggt ccgttggtcc atctttgtgg atcccaccgc ggctggtaat aacgatggct
      901 tttttacccg ttaccagacc ttccggaccg ttctcggtat agcggaaagt aacgcctgcg
      961 cgtgcaacca ggtcaaaata gtttttcaac tgagttgaga tgttgaagtt atacatcggt
     1021 gccgcaataa cgataacgtc gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc
     1081 agagcttcct gctgacgcgg agtcagcggc gcatcgctcg gacgcagagc gccaaccagt
     1141 tcgccatcca gtaccggaat cggatttgca gctaggtcgc gaacggtgat ttcatcagcg
     1201 gagtgctttt cgctccattg ttcaacaaaa taatcggaca actgattaga ctgagagtac
     1261 cctgccagga tgctggattt aagaactaat accttgctca tggtgtttcc ttatagatgt
     1321 ttgaatggac gatgccccgt tgcttgctga cactttattc acaatcctgc cacagagatc
     1381 gcgcaataaa tcgaagtcta tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt
     1441 ggtactctat atctatcatt taaaagaaaa ttaatcaggc agactactgc ccactaacgt
     1501 tatgacagaa caacaaaaat tgacctttac tgccttgcaa cagcggctgg attcgctgat
     1561 gctgcgcgac agactgcgtt tttctcgccg tctgcattgt gttaagaagg ttaaaaatcc
     1621 tgatgcacaa caggccattt tccaggagat ggcgaaagag attgaccagg cggcagggaa
     1681 agtcctgctg cgtgaagcgg cacgaccgga aatcacttat cctgacaatt taccggttag
     1741 tcagaaaaaa caggacatac tcgaagcgat tcgtgatcac caggtggtga tcgtcgccgg
     1801 ggaaaccggc tccggtaaaa cgactcagct accgaaaatc tgtatggagc tggggcgcgg
     1861 tattaaaggg ctgatcggtc atacccagcc gcgtcgcctg gcggcacgaa cggtggccaa
     1921 ccgtattgcg gaagagctga aaacggagcc gggcggttgc atcggttaca aagtgcgttt
     1981 cagcgatcac gtaagtgata acacgatggt caagctgatg accgacggga tcctgctggc
     2041 ggagatccag caagaccgcc tgctgatgca gtacgacacc atcattattg acgaagcgca
     2101 cgaacgcagc ctgaatatcg attttctgct cggctatttg aaagagttgc tgccgcggcg
     2161 tcctgaccta aaaatcatta tcacttccgc gactatcgac ccggaacgct tttcgcgcca
     2221 ctttaataat gcgccgatta ttgaagtctc cggtcggacc tatccggtgg aagtgcgcta
     2281 tcgcccgatt gttgaagaag ccgatgacac cgagcgcgat cagttgcagg cgatttttga
     2341 cgccgtagac gaactaagcc aggaaagccc cggcgacatt ctgatcttta tgagcggcga
     2401 gcgggaaatt cgcgataccg ccgatgcgct gaacaagctg aacttacgcc ataccgaaat
     2461 cttgccgctt tatgcgcggc tttcgaacag cgaacagaac cgcgtgttcc agtcgcacag
     2521 cggacggcgc attgtgctgg cgaccaacgt cgcggaaacc tcgctgaccg taccggggat
     2581 taaatacgtc atcgaccccg gtacagcgcg tatcagccgc tacagctatc gcaccaaagt
     2641 gcagcgtttg ccgattgaac ccatttcgca ggcgtctgcc aatcagcgta aaggccgctg
     2701 tggtcgtgtg tccgaaggga tctgtattcg tctctattcc gaagacgatt tcctctcgcg
     2761 cccggagttt accgatccgg agattctgcg taccaacctg gcctcggtta ttttgcagat
     2821 gaccgcgctg gggctgggcg atatcgctgc gttcccgttt gtcgaagcac cggataaacg
     2881 caatatccag gatggcgtgc gtctgctcga agaactgggc gcgatcacca cggatgaaca
     2941 ggccagcgcc tataaactga cgccgctcgg tcgccagctc tcgcagttgc ctgtcgaccc
     3001 acgtctggcg cgtatggtgc tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat
     3061 tatcacgtcc gcgctctcca tccaggatcc gcgcgaacgt ccgatggaca aacagcaggc
     3121 atcggacgaa aaacatcgtc gcttccacga caaagaatcc gacttcctcg cgtttgtaaa
     3181 tctgtggaat tatcttggcg agcagcaaaa ggcgctttct tccaacgcct tccgtcgcct
     3241 gtgtcgtacc gattacctca actatctgcg cgtgcgcgaa tggcaggata tctacaccca
     3301 gttgcgccag gtggtgaaag aacttggcat tccggtcaac agcgaaccgg cggagtatcg
     3361 tgaaattcac atcgccttac tgaccggttt gctttcccat atcggcatga aagatgccga
     3421 taaacaggaa tataccggtg cacgtaacgc gcgtttctcc atcttccccg gttctggttt
     3481 attcaaaaag ccgccgaaat gggtaatggt ggcggaactg gtagaaacca gccgcctgtg
     3541 ggggcgcatt gctgcgcgta tcgacccgga atgggtagaa cccgttgctc agcatttgat
     3601 taaacgcacc tacagcgaac cgcactggga acgggcgcag ggcgcggtga tggcaacgga
     3661 aaaagtcact gtttatggtt tgccgattgt tgctgcgcgc aaggtcaact acagccagat
     3721 cgattcggcg ttatgtcgtg aactctttat tcgccacgct ctggtggaag gtgactggca
     3781 gacgcgtcac gcattcttcc gtgaaaacct gaaactgcgg gccgaagtgg aagagctgga
     3841 acacaaatca cgtcgccgcg atattctggt tgatgacgaa acgctgtttg agttctacga
     3901 ccagcgcatc agccacgatg taatctccgc tcgtcacttc gatagctggt ggaaaaaagt
     3961 cagccgcgaa acgcctgatt tgctcaactt tgaaaagagc atgttgatca aagaaggggc
     4021 ggaaaaaatc agcaagctgg attacccgaa cttctggcat cagggcaatc tcaagctgcg
     4081 tttgagatat cagtttgagc caggcgcgga cgcggacggt gtgaccgtac atattccgct
     4141 gccgttactt aaccaggttg aggaaagcgg gtttgaatgg cagatccccg gtctgcgccg
     4201 cgaattggtg attgctctga ttaaatcgtt gccgaaaccg gtacgccgta attttgtgcc
     4261 tgcaccaaac tacgccgaag cgtttttagg ccgcgtcaca ccgctggagt taccgttgct
     4321 cgacagcctt gagcgcgagt tacggcggat gaccggcgtt accgttgacc gcgaagactg
     4381 gcactgggat caggtgcccg atcacctgaa aatcaccttc cgcgtggtgg atgacaaaaa
     4441 caagaagcta aaagaagggc gctcgctaca agatctgaaa gatgcgctga aaggcaaagt
     4501 gcaggaaacg ctgtccgcgg tggcggatga cggtatcgag cagagcggct tacatatctg
     4561 gagttttggt cagctgccgg aaagttacga acagaagcgt ggcaactata aagtgaaggc
     4621 gtggccggcg ctggtggatg aacgcgacag cgtgacgatc aaactgtttg ataacccgct
     4681 ggagcaaaaa caggcaatgt ggaatggtct tcgccgtcta ctgctgctaa atatttcctc
     4741 gccgatcaag tatttacatg agaagttacc aaacaaagcc aagctggggc tgtactttaa
     4801 cccgtatggc aaagtgctgg agctgatcga cgactgtatc tcctgcggtg tggatcaact
     4861 gatcgacgcc aatggtggcc cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa
     4921 agtgcgcgcc gaactgaatg acacggtggt ggatattgcg aagcaggtcg agcaaatcct
     4981 cactgcggtg ttcaatatca acaaacgtct gaaagggcgg gtggatatga ccatggcact
     5041 ggggctttct gatattaaag cgcagatggg cgggttggta tatcgcggtt ttgtcactgg
     5101 taacggcttc aaacggctgg gcgacacgct gcgatatttg caggcgattg aaaaacgact
     5161 ggaaaaactg gcggttgatc cacatcgcga tcgtgcacag atgctgaaag tcgaaaacgt
     5221 ccagcaggcg tggcagcaat ggatcaacaa actgccgcct gcacgtcgtg aggatgaaga
     5281 cgtgaaagag atccgttgga tgatagaaga gctgcgcgtt agttacttcg ctcaacaact
     5341 tggtacgcct tatccgattt cagataagcg tattttgcag gcgatggagc agattagcgg
     5401 ttaaccctgc tatttgccta ataaatctaa aacccggtaa gcatttagcg ccgggttttt
     5461 tattaattct aaaacggtaa ggacaaaaat tcaggaattc agaaaaatac aattctcagc
     5521 tgcaagatga ataatgttta tctacagc
//
LOCUS       DAJTUI010000172         5523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTUI010000172 DAJTUI010000000
VERSION     DAJTUI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.172"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..65)
                     /locus_tag="OO464_003877"
     CDS             complement(<1..65)
                     /locus_tag="OO464_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCR6228836.1"
                     /translation="MDQMEKITTGVSYTTSAVGTG"
     gene            complement(215..376)
                     /locus_tag="OO464_003878"
     CDS             complement(215..376)
                     /locus_tag="OO464_003878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228837.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(373..561)
                     /locus_tag="OO464_003879"
     CDS             complement(373..561)
                     /locus_tag="OO464_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6228838.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            821..1156
                     /gene="iraM"
                     /locus_tag="OO464_003880"
     CDS             821..1156
                     /gene="iraM"
                     /locus_tag="OO464_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6228839.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1437..1545)
                     /locus_tag="OO464_003881"
                     /pseudo
     CDS             complement(1437..1545)
                     /locus_tag="OO464_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2068..2144)
                     /locus_tag="OO464_003882"
     tRNA            complement(2068..2144)
                     /locus_tag="OO464_003882"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2108..2110),aa:Arg,seq:tct)
     gene            complement(2246..2321)
                     /locus_tag="OO464_003883"
     tRNA            complement(2246..2321)
                     /locus_tag="OO464_003883"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2286..2288),aa:Ile,seq:cat)
     gene            complement(2466..3287)
                     /locus_tag="OO464_003884"
     CDS             complement(2466..3287)
                     /locus_tag="OO464_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6228840.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3302..3658)
                     /locus_tag="OO464_003885"
     CDS             complement(3302..3658)
                     /locus_tag="OO464_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6228841.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3671..4720)
                     /locus_tag="OO464_003886"
     CDS             complement(3671..4720)
                     /locus_tag="OO464_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6228842.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            complement(4722..5000)
                     /locus_tag="OO464_003887"
                     /pseudo
     CDS             complement(4722..5000)
                     /locus_tag="OO464_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5067..5318)
                     /locus_tag="OO464_003888"
     CDS             complement(5067..5318)
                     /locus_tag="OO464_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228843.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
BASE COUNT         1484 a         1476 c         1156 g         1407 t
ORIGIN      
        1 tagcccgttc ccaccgctga cgtggtgtat gacacacctg ttgtgatttt ttccatctgg
       61 tccatacccc gtctcccgtt atccggaagc tgacaacaat aaaaaagcca ccagttaact
      121 actgatggct ctgataactc atgcaagcgt ctcagacgac ccactgacac taccggtgag
      181 tttaacgata ccttccattt gactggctca ctttttatga tgatgccggt gcatttatct
      241 ccagcaccag actttctatc tcaacgccat acgttgcatt tttggtaata tccgtcagcg
      301 tcagtgcatt tagtcccact gccagactgt cttttatggc ctggaatgcc gggccagtac
      361 gatgacgtag tatcactccg gctcagttgc accactgacc accacatcac cttctgctgc
      421 aatcgcctgc atcagggtat aaggggttat ggccaccgga ctaccaaacg gctgccagcc
      481 ctctttcagt ttatgtgtca gcttttccgc aagatctgac ggcggcgccg ccctgacaac
      541 atcatagtgt ttaaatgcca tggttctttc caacatctga aaaataattc tttaaaatac
      601 ctgacatgta atacagaaaa aacacaaaac cataccttaa ataaaaacct gattatcaag
      661 cagatatgca tggataaact acaagacgag atataaacca ccctgcattt aaataaacaa
      721 taaacaacat cagaaaaata attctgttct atggtttaat tcaaaaatat catttatact
      781 tttcagaaca tcaccagcaa ggcataaaca aggaaactaa atgaagtgga ttgtgattga
      841 tacagttatc cagccatcat gcggaatatc tttttcagtc atatggagta aaataaaatt
      901 aataatctgg tatcaatcgg atgctttctt acctcctgaa agtatattta cactgactca
      961 cacaggcatc atgctcaata acaaagtgct acctgtaacc atttacaacg tagtaccatt
     1021 caataaaaca ttctggaatt taatcaaaaa cagccaggaa tgccctacaa atacagataa
     1081 cgtattgaat gaatgcttta ataaccgttg cactctgcaa atatgccctt atggactaaa
     1141 acaacaaagt ccataaggag tttactcaca tctgacaaaa tcaatataaa cagcccctcc
     1201 ggagaggggc tggagagtgg cgctatgtgc cattccatgg tgccgggtgc ctcccggtga
     1261 attcagtacc agcacctgaa tccgcgatta tcccatatac ctactcgctg attgcccctc
     1321 cgcacagggg gattcaccat gccagtttct tttaacaaac tccccgcaaa ccagacaaca
     1381 gtcaaccgcc tgaattgtga agtatttaaa aatttctccc gctaactgat acccggctaa
     1441 cagtctggcg ttttcttttt cagcaacggg aaagcaacaa ccaccacacc cgccaccagc
     1501 acaccgtcag ccggcactga cattatccgg ctgctgcaat gccattaaca aaaacagtaa
     1561 gcaatcactt tttaccgtaa caggtgataa tccagatatg tatctacccc agatgagtaa
     1621 tccgaagttc atccatacca caggtcctgg ctattctgtt gtactcctga acaagagcaa
     1681 ataattctga attagcaacc atgaactcat cgcaaaccct ctgtatagca tcactattca
     1741 gaataataac gtctcttccc gaaagacgat caggagtaca gaacaaaact gtcaaacggc
     1801 tgaaggcctt tgctcgtgct gcattgacta tatcaatacg ctgcctaagg atgaaacacc
     1861 ccgacgcctc atcaatattc actctaccca caccatatga atgataaata cttaatgctg
     1921 aaaaaaccat tagaccgtat aacaaatact caatcaatac ttaacagaac ttttattttt
     1981 gacaaatata taatattttc aacaatatcc tgagccaggt atatttcagt ataaggctct
     2041 gccggaagga atctggaaga atgaatatgg cgcgctgtac tggattcgaa ccagtgaccg
     2101 attgcttaga aggcaattgc tctgtccggc tgagctaaca acgctgaata ccgataatgg
     2161 accgccatcg gggacccgcc ccccgcacca acaaccctgt tatcgtgtcg tctgctcttc
     2221 ctgataagct aatggcggtt tgtgatggtg gcccttgctg gatttgaacc agcgacctgg
     2281 cgattatgag tcgctcgctc tcaccactga gctaaagggc cgggagcaga ataataacgg
     2341 tccgtaatta atttcgcaat aaaaaaaccc gctcagcggc gggttgtaga aactcttcta
     2401 acgccaggca taaaacgccc atcattatga cgaatttacc acagattccg gaaaaatcaa
     2461 ccttgttacc tagttacctt ttttaactgc cgctcagccc atgcttcttc aatatcaaac
     2521 cgggtcacca gcgcatcata aaatttctta actgtttttt cccatgacgc gcgtgttatc
     2581 tggtttgtca cctcgcatat agcattaaat gcctccgttg atggtagtct ttcatagcca
     2641 cgaccaccac aacgctggca gtctctgata acaggcatac cacgttttac cgactcttca
     2701 cgatgaatgg cgacaccacg cccacggcaa tccttacagg cggtggaaac ctcacccttt
     2761 ccgccacact ccggacaggc aacttttacc acctccctga cttttttcca ttcttcccag
     2821 taaaacggat acacaccttt cgtacacttt gcccataccg gcggcttacc atccggatac
     2881 tggatcttgt ttgtaaaaac tatgctttca ataaattttt ccccatagca acaagggcac
     2941 tgctttttac tcgctgcgct gcgggcataa tcctcaaaag cgtacgaagc cataatgcgc
     3001 atcactaccg gttttatttc tgccggaagt tttctcaacg ccgccacacg atcgcaccga
     3061 ctgagtgcat aatctgccag taattctgtt gcccgcgccc tgtcattcat actgatgccc
     3121 attttcccca ggaacgcaga aaaacccatc tcagcccgat tctgtgtcat gccctgcgcg
     3181 gccatcacat cagtgatact cagcgcatct tttgacgttg aggccgatgc atcggtcagg
     3241 ccaggggatt ttggggagta gtatttcggt aaatcttcca gtttcatttt ttgacctgct
     3301 cttaatgcat tatggggtaa atcttcaccc ccagacgtcc accagatact ggctgaccac
     3361 gaacgatatt gatttcatca aactgctcat cgtccattaa cactcccgca tgcgtcagcg
     3421 catccagcgg tgctttcagg atattgtcca ggtcgcgacg acgcttatcc ggtggctctg
     3481 caatcacctt tatcgccagc cttccggaca ggcttaattt cagccgctgc tggcgaacaa
     3541 taagcgccac agcccggcga taacgctttc cctcctccga gataaaatat gtgctgccac
     3601 ggcgtcgcca gtaagtgttc accgtcggcg ggtaaggtaa aaccaaatct atgagcatca
     3661 gtcacctctt ttacccaagc acgccagttg caaaggcgtg atcaagaaaa cgaaaaatta
     3721 aatcaacctg agaaccatgc ttttcttcga acgccagagg atccgcatga agctcgttgt
     3781 gatgctcccg acacagcggt agcgtgaaaa tatcgtgaga tttggtcccc attccgccct
     3841 gaccatgacc aatcaggtga tggggatcgt cggctggctt accacaacac gcacacggct
     3901 gtgtcttcac ccagcgtttg tatttctcgt taacccagcg gcgacgttta ggtcgtttca
     3961 tgaaagattc cggagactca ggatcaacgg caatgctgac caccgtcttt tcctgttgtg
     4021 ggttctgttg ctggtgggcg tgaggcagcg gcgcaagatt ttttgtgcgc tgcttcagta
     4081 tgctggtggc ggtctgctct cccggcacga tgtcgctttc acggtacatt gagcggattt
     4141 tttccgcacg caaccccagc gaacgacgta ataccgcttc cggtagcgcg tccgccacct
     4201 gattgcggac cgcccaccag gataattcag ccagagataa ttcacgctcc tgcgtaccgc
     4261 ttattgcgtg accgatgacg tcaatcatcc atgctgacag gttttgatga gcaagttgct
     4321 cgagtgattc ggatgtctgg tcacgcagct ggttgtcgca gtgccagcac aataccattg
     4381 cgccggtacc ataacggtga atgacggttt cgctgtgatg ataatcgccg tgtggccact
     4441 ggcaggattt aatatggcgc aacagccagt cagacaatgc gccagcacca ccagcagcac
     4501 gaatcacccg tgcgttactg aaaaacggca gcaatgtttt gtcttccact agcggctggc
     4561 gaacggcagg aacgaccccg gacggcagat tacgcatgct tttcggttcc ggctccacca
     4621 gtacccgggt attgtggaat accggcatgg attcacgacc gggcttaagg accaccagcc
     4681 cgagttccgg aaccagaaca ggtcgaagta atacccgcac gttacctcca gatccgttgc
     4741 tggaatgtgc gggacggacg cggtggctgt tcggagtaag ggagcctgac ggagattatc
     4801 tagtgacggt agtcgaggct aagggctttt ttaacctcgc atccgcgcct gcggtaacac
     4861 tgaataagcc attcggcctg ttcttcagtg catgagggat gctggtacca gtctgactta
     4921 aatgcgtgag aataccgctc gtgcgtgtgg gcaagaacgg tcgaattatc atgattgtaa
     4981 tattttgcgt tgcgtgccat cggttttctc cggtggcacg gtgttactca gcgggagttc
     5041 agccccgcgc aagattgtag atgagtttat tcttctgaaa aagcagaaaa gccagctttt
     5101 attccgatct ctttcaatgc ctgtaatgaa gtgacaaact caccgtcgcg caagataaat
     5161 ccgtccgtca ctcgggcatc cacaaaatta attaacgcag ccccattttt ttgcaaacac
     5221 acaatgcggt aatgactaac aatatttcca ttttcaacgc acacagcata gaggccatct
     5281 tcacaaaaaa ttttacgcag ttcttcgatg ttcatcatca gaatccttcc agataagtag
     5341 cactcccctg ttcggggtcc atccctttta tccctgcgcg ctacttaagt atttttgatt
     5401 ctattccggc accatccaga acttcaaatg cgttgaaaat aaaaacaaaa aaccgccgaa
     5461 gcgggttaag tgcgggtgcg ttgaggatgc ctgccacatc agaggtggcg agggatttct
     5521 cac
//
LOCUS       DAJTUI010000173         5486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTUI010000173 DAJTUI010000000
VERSION     DAJTUI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.173"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(144..1853)
                     /locus_tag="OO464_003889"
     CDS             complement(144..1853)
                     /locus_tag="OO464_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR6228844.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2285..3004)
                     /locus_tag="OO464_003890"
     CDS             complement(2285..3004)
                     /locus_tag="OO464_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR6228845.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3129..3685)
                     /locus_tag="OO464_003891"
                     /pseudo
     CDS             complement(3129..3685)
                     /locus_tag="OO464_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3669..3927
                     /locus_tag="OO464_003892"
                     /pseudo
     CDS             3669..3927
                     /locus_tag="OO464_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(4036..4614)
                     /locus_tag="OO464_003893"
     CDS             complement(4036..4614)
                     /locus_tag="OO464_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228846.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(5035..5272)
                     /locus_tag="OO464_003894"
                     /pseudo
     CDS             complement(5035..5272)
                     /locus_tag="OO464_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         1556 a         1180 c          954 g         1796 t
ORIGIN      
        1 tcaggaagga gtgtccgtta aactcaggct acctcaggtt gccaattgag gtggttgcct
       61 gaaagttggc agttacccgg tcactacacc ttgttggagt gaacatatca aaaacaggta
      121 attacacaat ttaaattaca gtctcataaa aaatgattaa atctatttat tatttcaata
      181 aacgtttttt tcatcttctt tgaaaattgt tctgctatag tatccggttc cagattgttc
      241 tcttttatta ttctacaata ctctaatatt gtttctttgt ctctatttac tacagataca
      301 aatacaccag gaataccatc tttattagtt gcggacagca tttcgaccaa tcgtcttgtg
      361 gtaaaactat agttttttag aatattacaa aaggcacgta ttgtttcggt atggccatta
      421 taaaatgcca taaaaagtcc tgatgtaccg ttgggatttt ttatccataa taattcatct
      481 atatattcct ggtgaggggg gagtattttt agtatatctc catacgccat aatggccttt
      541 gtgtgcccat tttgaagtgc cataaacaac ccatgcttta ctttcaataa ttctttaatt
      601 tgttcactac taagcatttt cttattaagc agacttccga acatacgaat actatctgca
      661 tgtccattct gcaatgcaaa aaataaccca ggtaaatcat ggctgtcttt cgcagctaat
      721 agatataatc tcttatcgga agttaacggg gttttaaaga ctatttcccc ataggctttt
      781 attgtgtcta catgtccgtt cataaaagac atgcataaac ccggcacatt gtttgcccct
      841 accgcagcaa gcaatgtaag tattgtttct tgactaagat tgcacatatt taatatctta
      901 ccatatactt gaataatgtc tgaatatccg ttttgaagtg ctatataaaa tgcaggagta
      961 ccatcagagc ttctcgtggc cagaaggttc accagtttag attgagaaat ttcaccattt
     1021 ttgtattttt ccatcagtat aaataatact tctgtcagat tctttgttat cgcatgatat
     1081 ataactctgg aattgatcaa ttcatctggt aaaagtttca ctgtgtttgt tagaggggaa
     1141 ttatgcctat cttcaaatat tacctgcccg ggttgctcca gaccataaat tttccaataa
     1201 aaatcatcaa aaaaatcctt agcggataat tgactcagtt cgaaatggct atcacaagta
     1261 aatacagtac gtttatgagt aacagttcga ttaggatcga aaaactgaac aacccatttt
     1321 tcactttctt gtacgcgctt tattttcaac cctaacgcca tttggtggtt taatgtgcat
     1381 gctgataatg ttctgacaaa gtctccattt tttttcatgt tataaaaaaa attagtaata
     1441 actgttcccc actttttatt ttccacaaaa taaatataag ctggtgattt tgaaaaatct
     1501 tccgtttttt ccaaacatac attttttgca atagcatcag gggtgctaag aagctgatag
     1561 tttaccttcc catttgtttc cataaaattc atttcccaat aggaagctag gtgacggcaa
     1621 actatttgtc ttccatatat atcttttgcc actccattta tatttacctc cacatttctc
     1681 ttttctgaaa aataaggtgc tgttttattc ttaaactgaa ttagttcact ggagtttgag
     1741 accctcataa tagaacttgt tattgttcta ttatctctgt taacagacat atctgttgat
     1801 aacgaatttt ttgttgaaaa tatactagat gaaaatgttt tatttaacgg catagaagac
     1861 aaccatagag atgttataat actattagat tgcagttact aatagtaaaa gcctccttac
     1921 aaggaggtag cttattcaga atgctcctat aagatagcga gctcgtcaac gatacagatg
     1981 atctgattat attgtgcaaa aggagaacag gaaaataggc actggaaaag atgaaggcct
     2041 gatgggagca ttaactaatg caaaatccga caggttaata tcccgaaaag caatcaaacg
     2101 actcaaacag gagatgcacg ctgaaatggt ggacaactgt tcggatttca gatgagtagc
     2161 tcagcagttt atgagccgaa tgctctgtca cgggcaggaa tcaggctgca tacgcagtgc
     2221 caaatctaat cgtagttatt ctaaccagtt ttcaagctac aaggtggtgt agctggcatc
     2281 ttctctactc tatcatcaaa cgataaaatg gttcctctcg taacatttgc tcttgccttt
     2341 cacttccatc tcgatcgctt cgtagttcat tacgatagct gacatatttc cagtcttcag
     2401 gtctaacatc tgacgccgga tactccccgg gagcgatttt gcttcttaat agattagact
     2461 cgagacacat tataaattgt cgtattttat gaagtaataa agcactatat tgcgagcatt
     2521 cctgatctga ttttacatat agcgtaaact gagctcctat ccccacacga gattgctgag
     2581 aaactcgatt catatcagtt atcttccatt tatctatagg actgtcctct gaaaataata
     2641 aaccagacaa aatttggaat gccagaggaa cttgctccct agctatacta atatgaaact
     2701 tgtccccaac aaaatccccc ttactctgag gattttcacg acatgcatga ataaacacat
     2761 ctccactatg gttcacaatc tggaatcctt ggtacatgac agaaaaagat tgacatatat
     2821 cagagtaagt tggcacgttc gatgctactt tgaaatagta cgctgggtat tgccgtattt
     2881 ggttgtacaa tatattgaag ttatttttca atctttcatt cgcagaaagc atctgcggta
     2941 tttcagacct cacaacattc aaagaatcta aatttagttt tagacagggc ttttttatgg
     3001 gcatagaaaa cgtcctctat aaaatagata tattatctta tattgattgt ttatcatctt
     3061 ttataaaacg atacattata tgtaatgtat gtaagcgtaa gggtcctctc cgaacatggt
     3121 gggaaggctt agaacgccat attcagtttc ccatagtgga acatcactcc cagcttaaag
     3181 cgctcccggt tgcggtagcc cttggctttt attctcagca gcctgatctt gctgttcagt
     3241 gcttccgcat ttccgtttga tacgccatgg cgcatggcgt tcaggatacc gtacaacctt
     3301 ttccctatcg ttttagccac attcttcatc atggggacgt cactgttggc cgcagccatc
     3361 tctgccagtc gttgcgtctt tcctcgctcc atggtctgct ccagatatcc tttgccagtt
     3421 ctttcagcgc ccagcacagg ctcgtcagct tcatctgcgc acgcagccat atcagctttt
     3481 cctgcctgga ttcggtcatc catttatcgc tgtgctgcca caggaagcga gttccctttg
     3541 cctgacgacg actttcaaca ggaagatgcg gatgttcatt ctggcgggtt ttatccacca
     3601 cctcacccgg ttgtttcgcc acatgaaagc gatcgaaggc tattttctca accgcattgg
     3661 gtaagtggat gcgcgctgct cttagtttgc tgagccagtt tagcattgcc ttctacatga
     3721 ccgatgcctg agccggtata ccgaacttta ttgtcctcaa ccagtcatgt tataagcaag
     3781 aaatgcactc agcggataga gcgacataat ctgaacctgc ggacacatct taagcggctt
     3841 acccgcaaaa ccatatgctt ctcaaagtct gatgatatgc actacaagat catcggctgg
     3901 tatctgacaa tcaaccacca ccactaaatc tgcgtcacga cctgcaactg agtctggttg
     3961 aacaggctga ggccctgctg aagcaacgcg aacctctcga ctcttgttgg attttaccaa
     4021 atagtttttc taaaattaaa cacatttata taagttcttt gctgaacaaa acgcagcccg
     4081 ccatcctttt tcattcataa attcagcaat ggcagataaa actttttcat caagcatata
     4141 aaaaataatg ccagtagact caatatgcct catacaaata aggccgcaca tatccgtaat
     4201 atatttgtca atgctgctac catttgcatg gtaatatgtc atgctattat ctgatgattt
     4261 tacatatgca gtatgacata tttttccttc acgattaaca aaactgacaa aaccactttc
     4321 accaggagta atatataaag atgaatttaa cgtgcgctca ctaatattca ttaattgttg
     4381 aaattcaatg gaatagtttt cagttaatgt atctattatc ttggctttgt ctttctttgt
     4441 tatctcattg tctttttcgg caagaaaaat tgccacttta tgacatgcat tcagctcgtt
     4501 aattttacct gataaaactg cttttcttac cacatcgttg aactcatcga tagtgtgttt
     4561 tttattatat atatcatgaa aaatatagcc taaagactta aacgagactt tcattttata
     4621 ttctccttaa atattaacag gatatatata tcacattttc ctctatattt tcagaaacca
     4681 gctatcattt tccccattac tggtacgtcg cttcagatat ttccgcagca agcgataacc
     4741 ctgcgcacta tgaggatgcc attcattata atgtttgaac accttcgcga gactacttta
     4801 ctgctgttaa cacgtcaggt tcgaacataa cactgattta atcatatttc atcgttttga
     4861 cgaagttttc tgccattcca ttttgcaccg ccgtgtgttt gggggcaagg ccgaccatct
     4921 gtgtttcatt cgccagatat aagtgatgag atggcattct ccttccttca cggcagaagc
     4981 attttgaccg tttaggggat gcggacgatt ttcatgcctg ctaagatact ttcgttactg
     5041 atgctgtccg ttttccacca tttcatcatc actgattggc gttgtgtttt tggtcagcac
     5101 tccagcttta cgtttctctt ttaaccggta actctctcct ttgatattca gcgtgtagca
     5161 gccgatctag gatcgctgtt gccagcacgt gatctccgaa catttctccc cagtctgcga
     5221 cccctttgtt tgacgtcagt atgatgctcg ctttttcata tcgacagttc atcgatagga
     5281 atttaaatcc ccaaaagatt aaaaaagcac cgaagaatgc gcgtttattc aacacaacct
     5341 gatcatcaag taatcggtat agacacttaa tattgcttac attgattgat tcaatgtatc
     5401 tctgatggtt ttttgctccg ttcccctcaa catggacgca acttcccctc tgcgactctc
     5461 agccgcacca ccgtattcga gccagc
//
LOCUS       DAJTUI010000174         5391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTUI010000174 DAJTUI010000000
VERSION     DAJTUI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.174"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..487
                     /locus_tag="OO464_003895"
     CDS             <1..487
                     /locus_tag="OO464_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR6228847.1"
                     /translation="FPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIAN
                     EPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAA
                     PSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESA
                     H"
     gene            509..2500
                     /gene="cyoB"
                     /locus_tag="OO464_003896"
                     /pseudo
     CDS             509..2500
                     /gene="cyoB"
                     /locus_tag="OO464_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2490..3104
                     /locus_tag="OO464_003897"
     CDS             2490..3104
                     /locus_tag="OO464_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR6228848.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3104..3433
                     /locus_tag="OO464_003898"
     CDS             3104..3433
                     /locus_tag="OO464_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR6228849.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3445..4335
                     /gene="cyoE"
                     /locus_tag="OO464_003899"
     CDS             3445..4335
                     /gene="cyoE"
                     /locus_tag="OO464_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR6228850.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4519..4830
                     /locus_tag="OO464_003900"
     CDS             4519..4830
                     /locus_tag="OO464_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6228851.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4814..4972
                     /locus_tag="OO464_003901"
     CDS             4814..4972
                     /locus_tag="OO464_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6228852.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4965..5363
                     /locus_tag="OO464_003902"
     CDS             4965..5363
                     /locus_tag="OO464_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6228853.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1174 a         1342 c         1424 g         1451 t
ORIGIN      
        1 tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga tgaactcctt
       61 cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga ctcgcctgca
      121 tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca gcgggccggg
      181 cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat tcgaccaatg
      241 ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt tcgaaaaact
      301 ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac cagacttgtt
      361 tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga cccagccaga
      421 aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg cggaatccgc
      481 ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg atgcagtccc
      541 gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag gtctggcgct
      601 cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt ggctgacctc
      661 cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga tgttgctgcg
      721 tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg cgggtgaagc
      781 gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg tgattatgat
      841 cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc cgctgcagat
      901 cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt ttactgttgt
      961 tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga ccggctggct
     1021 ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg attactggat
     1081 atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact tcttcgttac
     1141 cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat ttacctaggc
     1201 atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg ttaccgtcgc
     1261 gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata tgggtggcaa
     1321 catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt acatcctgat
     1381 cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta aacgtctgtt
     1441 tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt tcatcgtttg
     1501 gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct ttggtatcac
     1561 cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt tcaccatgta
     1621 tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta tcgtcacctt
     1681 ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact tcgttctgca
     1741 taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg tggtcttcgg
     1801 ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac tgaacgaaac
     1861 ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct ttatgccgct
     1921 gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg acccgcagtt
     1981 ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg gtattctctg
     2041 cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg acctgactgg
     2101 cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc cgttctataa
     2161 ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga aagagaaagg
     2221 cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa acagcggtgc
     2281 aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct ggcatatctg
     2341 gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga aaagcttcga
     2401 cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa accagcattt
     2461 cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg cacgcgactg
     2521 cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc ggattttgga
     2581 tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat gccgttctgg
     2641 tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg ttcgttctgg
     2701 ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct atcgccatgt
     2761 acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg tttggtgccg
     2821 gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc atggggccgg
     2881 atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt ctgcacgtca
     2941 cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc ggcctgacca
     3001 gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg gatgtggttt
     3061 ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc attctaccga
     3121 tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta tcctgtcgat
     3181 cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc cggccgtaat
     3241 tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg tgtgcttcct
     3301 gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct tcaccgtgct
     3361 aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca actacaacat
     3421 gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt aacgaaacca
     3481 ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc ctcaaagggc
     3541 agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt tgtggcgtcg
     3601 ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga aaggacgaag
     3661 aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta cgccaccttg
     3721 ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc ctgctggctg
     3781 ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa acgccactca
     3841 gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat tggctactgt
     3901 gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt cagcctgtgg
     3961 cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca ggcggcaaac
     4021 attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat cacgctgtat
     4081 atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc tgggtataaa
     4141 tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct gcgcggttat
     4201 aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat catcgccatc
     4261 actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca tacgctgctg
     4321 gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct ttttattgtc
     4381 tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga tgcacataaa
     4441 ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga tttcattcag
     4501 ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt cgataaaacg
     4561 ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa acttcgtgaa
     4621 gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc tatggaacgc
     4681 gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag ctgggaactg
     4741 atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc gttaatgaaa
     4801 aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag tcctctcctg
     4861 taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg gtaaccctgt
     4921 tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt aatagcgact
     4981 gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc tgtactttta
     5041 cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca caaccttcag
     5101 aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa aagcggggct
     5161 ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac ccggcattac
     5221 caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat gctcgtctcg
     5281 gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg tttttggcga
     5341 ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggcaag t
//
LOCUS       DAJTUI010000175         5390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTUI010000175 DAJTUI010000000
VERSION     DAJTUI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.175"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..241
                     /locus_tag="OO464_003903"
     CDS             <1..241
                     /locus_tag="OO464_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228854.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="OO464_003904"
     CDS             complement(238..1179)
                     /locus_tag="OO464_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR6228855.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="OO464_003905"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="OO464_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="OO464_003906"
     CDS             complement(1684..2904)
                     /locus_tag="OO464_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR6228856.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="OO464_003907"
     CDS             complement(2908..3651)
                     /locus_tag="OO464_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR6228857.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="OO464_003908"
     CDS             complement(3542..5008)
                     /locus_tag="OO464_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR6228858.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTAMGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5241)
                     /locus_tag="OO464_003909"
     CDS             complement(5008..5241)
                     /locus_tag="OO464_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR6228859.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
BASE COUNT         1145 a         1517 c         1362 g         1366 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc catcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa
//
LOCUS       DAJTUI010000176         5388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTUI010000176 DAJTUI010000000
VERSION     DAJTUI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.176"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..935
                     /gene="tauA"
                     /locus_tag="OO464_003910"
     CDS             <1..935
                     /gene="tauA"
                     /locus_tag="OO464_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6228860.1"
                     /translation="AALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVD
                     WRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKK
                     TISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDI
                     DGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSA
                     IDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPV
                     NKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            948..1715
                     /gene="tauB"
                     /locus_tag="OO464_003911"
     CDS             948..1715
                     /gene="tauB"
                     /locus_tag="OO464_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6228861.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1712..2539
                     /gene="tauC"
                     /locus_tag="OO464_003912"
     CDS             1712..2539
                     /gene="tauC"
                     /locus_tag="OO464_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6228862.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2536..3387
                     /gene="tauD"
                     /locus_tag="OO464_003913"
     CDS             2536..3387
                     /gene="tauD"
                     /locus_tag="OO464_003913"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR6228863.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3493..4467)
                     /gene="hemB"
                     /locus_tag="OO464_003914"
     CDS             complement(3493..4467)
                     /gene="hemB"
                     /locus_tag="OO464_003914"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6228864.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            4991..5359
                     /locus_tag="OO464_003915"
                     /pseudo
     CDS             4991..5359
                     /locus_tag="OO464_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1253 a         1356 c         1490 g         1289 t
ORIGIN      
        1 ttgccgcact ggcattcatc gcttttcagg cacaggcggt gaacgtcacc gtggcgtatc
       61 aaacctcagc cgaaccggcg aaagtggctc aggccgacaa cacctttgct aaagagagcg
      121 gggcgagcgt ggactggcgt aagtttgaca gcggagccag catcgtgcgg gcgctggctt
      181 ctggcgacgt gcaaatcggc aacctcggtt ccagcccatt agcggttgca gccagccaac
      241 aggtgccgat tgaagtcttc ttgctggcgt caaaactggg taactccgaa gcgctggtgg
      301 taaagaaaac tatcagcaaa cccgaagatt tgattggtaa acgcattgcc gtaccgttta
      361 tctccaccac ccactacagc ctgctggcgg cgctgaaaca ctgggggatt aaaccagggc
      421 tagtggagat tgtgaacctg cagccgcccg cgattatcgc cgcatggcag cggggtgata
      481 ttgatggtgc ttatgtctgg gcaccggcgg ttaacgccct ggaaaaagac ggcaaggtgc
      541 tgaccgattc tgaacaggtc gggcagtggg gtgcgccaac gctggacgtc tgggtggtgc
      601 gcaaagattt tgccgagaaa catcctgagg tcgtgaaagc gttcgccaaa agcgccatcg
      661 atgcacagca accgtacatt gctaaccctg acgcgtggct gaaacagccg gaaaacatca
      721 gcaaactggc gcgtttaagc ggcgtgcctg aaggtgacgt tccggggctg gtgaagggaa
      781 atacctatct gacgccgcag caacaaacgg cagaactgac cggaccggtg aacaaagcaa
      841 tcatcgacac cgcgcagttt ttgaaagagc agggcaaggt cccggctgta gcgaatgatt
      901 acagccagta cgttacctcg cgcttcgtgc aataaaagga ggcgcagatg ctgcaaatct
      961 ctcatctcta tgccgattat ggcggcaaac cggcactgga agatatcaac ctgacgctgg
     1021 aaagcggcga gctactggtg gtgctggggc cgtccggctg cggtaaaacc accctgctga
     1081 atctgattgc cggttttgtg ccttatcagc atggcagcat tcaactggcg ggtaagcgta
     1141 ttgagggacc gggagcagag cgtggcgtag tttttcagaa tgaagggctg ctaccgtggc
     1201 gcaatgtaca ggacaacgtg gcgttcggcc tgcaattggc aggtatagag aaaatgcagc
     1261 gactggaaat cgcgcaccag atgctgaaaa aagtggggct ggaaggcgca gaaaaacgct
     1321 atatctggca gctttccgat ggtcaacgtc agcgggtggg gattgcccgc gcgctggcgg
     1381 cgaatcccca gctgttatta ctcgacgaac cgtttggtgc gctggacgcc ttcacccgcg
     1441 accagatgca aactctgctg ctgaaactct ggcaggagac gggcaagcag gtgctgttga
     1501 ttacccacga tatagaagaa gcggtgttta tggcgactga actggttctg ctttcatccg
     1561 gccctggccg tgtgctggag cggctgcggc tcaactttgc tcgccgcttt gttgcgggag
     1621 agtcgagccg cagcatcaag tccgatccac aattcatcgc catgcgcgaa tatgttttaa
     1681 gccgcgtatt tgagcaacgg gaggcgttct catgagtgtg ctcattaatg aaaaactgca
     1741 ttcgcggcgg ctgaaatggc gctggccgct ctcgcgtcag gtgaccttaa gcattggcac
     1801 gttagcggtt ttactcactg tatggtggac ggtggcggcg ctgcaactga ttagcccgct
     1861 gtttttgccg ccgccgcaac aagtactggc aaaactgctc accattgccg gaccgcaggg
     1921 atttatggac gccacgctgt ggcagcatct ggcagccagc ctgacgcgca ttgtgctggc
     1981 gctactcgcg gcggtggtga tcggtattcc ggtcgggatc gccatggggc taagcccgac
     2041 ggtgcgcggc attctggatc cggtaatcga actttatcgt ccggtgccac cgctggctta
     2101 tttgccgctg atggtgatct ggtttggtat tggtgaaacc tcgaagatct tactgatcta
     2161 tttagcgatt tttgcgccgg tggcgatgtc ggcgctggca ggggtgaaaa gcgcgcagca
     2221 ggttcgcatt cgtgccgccc agtcgctggg tgccagccgt gcgcaggtgc tgtggtttgt
     2281 cattttgccc ggcgcgctgc cggaaatcct caccggatta cgcattggtc tgggtgtggg
     2341 ctggtctacg ctggtagcgg cggagctgat tgccgcgaca cgcggtttag gatttatggt
     2401 tcagtcagcg ggtgaatttc tcgcgactga cgtggtactg gcggggatcg cggtgattgc
     2461 gattatcgcc tttcttttag aactgggtct gcgcgcgtta cagcgccgtc tgacgccctg
     2521 gcatggagaa gtacaatgag tgaacgtctg agcattaccc cgctggggcc gtatatcggc
     2581 gcacaaattt cgggtgccga cctgacgcgc ccgttaagcg ataatcagtt tgaacagctt
     2641 taccatgcgg tgctgcgcca tcaggtggta tttctgcgcg atcaagctat tacgccgcag
     2701 cagcaacgcg cgctggccca gcgttttggc gaactgcata ttcaccctgt ttaccctcat
     2761 gccgaagggg ttgacgagat catcgtgctg gatacccata acgataatcc gccggataac
     2821 gacaactggc ataccgatgt gacatttatt gaaacgccac ccgcaggggc gattctggca
     2881 gctaaagagt taccttcgac cggcggtgat acgctctgga ccagcggtat tgcggcctat
     2941 gaggcgctct ctgttccctt ccgccagctg ctgagctggc tgcgtgcgga gcatgatttc
     3001 cgtaaatcat tcccggaata caaataccgc aaaaccgagg aggaacatca acgctggcgc
     3061 gaggcggtcg cgaaaaatcc tccgttgcta catccggtgg tgcgaacgca tccggtgagc
     3121 ggtaaacagg cgctgtttgt gaatgaaggc tttactacgc gaattgtcga tgtcagcgag
     3181 aaagagagcg aagccttgtt aggcttttta ttcgcccata tcactaaacc ggagtttcag
     3241 gtgcgttggc gctggcaacc gaatgatatt gcgatttggg ataaccgcgt gactcagcac
     3301 tatgccaatg ccgattacct gccacagcgg cggattatgc atcgggcgac gatccttggg
     3361 gataaaccgt tttatcgggc ggggtgatac gagtgcggac ggttccctcg cccccttggg
     3421 gagagggtta gggtgagggg gcgttcactg tactttcaac aggttaactc cccctttctg
     3481 agaggaaaaa aattaacgca gaatcttctt ctcagccaaa tccagcgcaa agtagctgaa
     3541 aatcagatcc gcacccgcac gcttaattga acctaagctt tcgagcacga ctttctcttc
     3601 atctatagca cccgccagcg cggcgaactt aatcatcgcg tactcaccgc tcacctgata
     3661 cgcgccaatc ggcaattcag tacgttcacg cagctcacgc acgatgtcga ggtacgctcc
     3721 agcaggttta accatcaggc agtctgcgcc ctgggcttca tccagcagtg actcacgaat
     3781 cgcctcgcga cggttcattg ggttcatctg atagcttttg cggtcgcctt ttaatgcgct
     3841 tcctgcagct tcacggaacg ggccataaaa ggaagaggcg aacttggtcg aatacgacat
     3901 aatcgccgta tctttaaagc ctgcagcgtc cagcgcctga cgaatcgcct gtacctggcc
     3961 gtccatcgcg gcggaagggg cgatgaagtc tgcgcctgca gcagctgcaa ccacggcttg
     4021 cttgcctaaa ttttccagag tcgcgtcgtt gtcgacgcca tgctcgcaca gcacaccgca
     4081 gtgaccgtga gaagtgtatt cacagaaaca agtgtctgac atgacgatca tttctggcac
     4141 agtctgcttg cagatgcgcg acatacgcgc caccagtcca tcttcccgcc aggcgtcgct
     4201 gccggtttca tcggtatggt gagagatgcc aaaagtcatt acggaacgga taccggcgtt
     4261 ggcgatgcgt tcaatttcgc gtgccagatg tttctctgga atgcgcatca cgcctggcat
     4321 ggcttcaacg gctttgtagt cgtcaatttc ttcttcaaca aagatcggca acaccaggtc
     4381 gttaaggcta agtgttgtct cttcaaacat agcgcgcagc gcaggagatt tgcgcaggcg
     4441 acgagggcgt tggattaagt ctgtcatggt ctgcctgatg tttgtggaat taaggtgcac
     4501 agtatacctg aagcggggca ggagtgtttt acgaaagttg tcgcgatgtt gacaagaaga
     4561 gaatgaaaga gagaccagga catctttgta atagaaatag caataatttc atattcactg
     4621 aaaatatttt taatctttat ttatagctct tggttattat tttttggcgt ttggttgtgc
     4681 tgctataaat tgaataattc aaactttgtt gtatattgat aagatattta tctggtttgt
     4741 atttttgata tttataacgc gtatgcatca aaaataacat tatctgcaac atactgaaat
     4801 atcgtccttt tatcaatttg atgcgtgttt ttctccataa attatatatg tccacattcg
     4861 gacttagggg aaagaataat tgaaccattc gtctgtaacg cagcataatc gttagcgcga
     4921 aacataatat gttttctatg cattgataat tgatggatca acttattacg tccctgagga
     4981 gggatgacaa atgcactcct ggaaaaagaa acttgtagta tcacaattag cattggcttg
     5041 cactctggct atcacctctc aggctaatgc agcgaccaac gatatttctg gtcaaactta
     5101 caatactttc catcactaca acgacgccac ctatgctgat gacgtttact atgatggtta
     5161 tgtaggctgg aacaactatg ccgctgatag ctattacaac ggcgatatct acccggtcat
     5221 taataacgct accgttaacg gcgtgatttc tacctactat ctggacgacg gtatttctac
     5281 caataccaac gccaatagtc tgacaatcaa aaacagcact attcacggta tgatttattc
     5341 cgagtgcatg acaactgatt agactggccc cctgaatctc cagacaac
//
LOCUS       DAJTUI010000177         5327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTUI010000177 DAJTUI010000000
VERSION     DAJTUI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.177"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(23..760)
                     /gene="bamD"
                     /locus_tag="OO464_003916"
     CDS             complement(23..760)
                     /gene="bamD"
                     /locus_tag="OO464_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6228865.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            895..1875
                     /gene="rluD"
                     /locus_tag="OO464_003917"
     CDS             895..1875
                     /gene="rluD"
                     /locus_tag="OO464_003917"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6228866.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1872..2603
                     /gene="pgeF"
                     /locus_tag="OO464_003918"
     CDS             1872..2603
                     /gene="pgeF"
                     /locus_tag="OO464_003918"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6228867.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2733..5306
                     /gene="clpB"
                     /locus_tag="OO464_003919"
     CDS             2733..5306
                     /gene="clpB"
                     /locus_tag="OO464_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR6228868.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1308 a         1286 c         1453 g         1280 t
ORIGIN      
        1 cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg attttcgcta
       61 ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc agcggcagcg
      121 catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctatg cggttaacga
      181 cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca tatttcgcca
      241 gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga ctgttcggat
      301 agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga ggatcgcgat
      361 cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc atattggtca
      421 ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg cgaataaaac
      481 gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg tagatgagat
      541 ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac gcttccagtt
      601 gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc gcgtaaattt
      661 catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc aaaaacaggc
      721 ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga ctttcctcaa
      781 aatgtaatac gggagattct ctgttcctgc acccggttaa gaccagctac aatagcacac
      841 tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt atatatggca
      901 caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg cttagatcag
      961 gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg gatcctcgac
     1021 cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt attgggtggc
     1081 gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc gcaggatatc
     1141 cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc gcgcgaccta
     1201 gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt gcttcattac
     1261 tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga taaagacacc
     1321 actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt cgaatctttg
     1381 caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat gaccgctggc
     1441 ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat ggcggtgcat
     1501 ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg tgtgcacacg
     1561 cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca tatggcccat
     1621 atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc gccaaaaggt
     1681 gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct tcatgcaacc
     1741 atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc gcctattcca
     1801 caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca taaggatgaa
     1861 gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa ggtgttgcgg
     1921 cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca ctcaacctcg
     1981 gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga ctttttgctg
     2041 cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa gatgtgctta
     2101 agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc aatacgcctg
     2161 gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc aatcgagcgg
     2221 gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc gtgctggaag
     2281 agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta gggccggcaa
     2341 ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg gcagtagacg
     2401 ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat atttatcagc
     2461 ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc gaccgttgta
     2521 catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc ggtcgtatgg
     2581 caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg cgtgattttc
     2641 ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca tttaatctcc
     2701 agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt actaataaat
     2761 tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac caattcatcg
     2821 aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt agtcctttat
     2881 taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag gcattaaatc
     2941 gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat ctggtgcgcg
     3001 ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc tcgtcagaac
     3061 tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg aaagcaacag
     3121 gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt gaaagcgtga
     3181 acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc gaccttaccg
     3241 aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa attcgccgta
     3301 ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt gaacccggcg
     3361 tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc gaagtgccgg
     3421 aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg gctggggcga
     3481 aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt gccaaacagg
     3541 aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg ggtaaagccg
     3601 atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt gaattgcact
     3661 gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat gctgcgctgg
     3721 aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc attgcgattc
     3781 tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact gacccggcaa
     3841 ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg ccggataaag
     3901 ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac tcaaaaccag
     3961 aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa caggcgttaa
     4021 tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa gaactgagcg
     4081 acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag gcatcgcttt
     4141 ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt gaacaggctc
     4201 gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc ccggaactgg
     4261 aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg ttgcgtaata
     4321 aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt ccggtttctc
     4381 gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg caccatcgcg
     4441 tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt agccgtgcgg
     4501 ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca acgggtgtgg
     4561 ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac gaggcgatgg
     4621 tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg gttggtgcgc
     4681 ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg cgtcgtcgtc
     4741 cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc ttcaatattc
     4801 tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg gtcgacttcc
     4861 gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag gaacgcttcg
     4921 gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc cataacttcc
     4981 gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg ggtgaacagc
     5041 acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg gaagaacgtg
     5101 gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac ggttacgatc
     5161 cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa aacccgctgg
     5221 cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg gaagttaatg
     5281 aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggg
//
LOCUS       DAJTUI010000178         5194 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTUI010000178 DAJTUI010000000
VERSION     DAJTUI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.178"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            66..2627
                     /gene="mutS"
                     /locus_tag="OO464_003920"
     CDS             66..2627
                     /gene="mutS"
                     /locus_tag="OO464_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6228869.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2733..3385
                     /gene="pphB"
                     /locus_tag="OO464_003921"
                     /pseudo
     CDS             2733..3385
                     /gene="pphB"
                     /locus_tag="OO464_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3436..4203)
                     /locus_tag="OO464_003922"
     CDS             complement(3436..4203)
                     /locus_tag="OO464_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6228870.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4399..5194
                     /locus_tag="OO464_003923"
                     /pseudo
     CDS             4399..5194
                     /locus_tag="OO464_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
BASE COUNT         1268 a         1341 c         1360 g         1225 t
ORIGIN      
        1 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
       61 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      121 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      181 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      241 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      301 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      361 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      421 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      481 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      541 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      601 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      661 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      721 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      781 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
      841 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
      901 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
      961 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1021 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1081 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1141 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1201 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1261 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1321 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1381 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1441 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1501 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1561 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1621 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1681 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1741 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1801 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     1861 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     1921 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     1981 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2041 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2101 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2161 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2221 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2281 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2341 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2401 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2461 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2521 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2581 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2641 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2701 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2761 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     2821 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     2881 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     2941 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3001 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3061 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3121 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3181 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3241 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3301 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3361 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3421 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3481 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3541 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3601 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3661 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3721 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3781 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     3841 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     3901 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     3961 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4021 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4081 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4141 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4201 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4261 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4321 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4381 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4441 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4501 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4561 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4621 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4681 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4741 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4801 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     4861 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     4921 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     4981 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5041 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5101 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5161 gccaaagccc tgcacttccc gctgccattg gcct
//
LOCUS       DAJTUI010000179         5172 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTUI010000179 DAJTUI010000000
VERSION     DAJTUI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.179"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..736
                     /locus_tag="OO464_003924"
     CDS             <1..736
                     /locus_tag="OO464_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6228871.1"
                     /translation="SQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNVT
                     YPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLGS
                     GNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSFY
                     LSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGLS
                     ATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1070..3349
                     /gene="ydeP"
                     /locus_tag="OO464_003925"
     CDS             1070..3349
                     /gene="ydeP"
                     /locus_tag="OO464_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6228872.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3596..3799
                     /gene="safA"
                     /locus_tag="OO464_003926"
     CDS             3596..3799
                     /gene="safA"
                     /locus_tag="OO464_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6228873.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3867..4628
                     /gene="ydeO"
                     /locus_tag="OO464_003927"
     CDS             3867..4628
                     /gene="ydeO"
                     /locus_tag="OO464_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6228874.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5030..>5172
                     /locus_tag="OO464_003928"
     CDS             5030..>5172
                     /locus_tag="OO464_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6228875.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
BASE COUNT         1478 a         1102 c         1154 g         1438 t
ORIGIN      
        1 gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg atcatataaa
       61 cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat cgctgtactg
      121 gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg atattggtga
      181 taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg cggccggtgg
      241 agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa ttgcaacatt
      301 aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca gtgttgttat
      361 gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc cggactttcc
      421 tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc tctcatttta
      481 tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag cacctgatgc
      541 aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac tggctacggg
      601 agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt taagcgcaac
      661 ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca ttggtgtcac
      721 ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc acgaaaattg
      781 cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc cgcgcacaat
      841 tgaagccaga gtcttatttt ttatattgga aaatatgtta atttaaacac tatgagagat
      901 gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa aatagaaaga
      961 gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat gtactgtgca
     1021 tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga tgaagaaaaa
     1081 aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg tagcgaatgc
     1141 agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca tgaataagcc
     1201 agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg cgtcattcga
     1261 catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc aggtaaatgc
     1321 ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc acgagcttga
     1381 ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact gttacaagcc
     1441 attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct atagtgatcc
     1501 caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct ttctttatca
     1561 gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca tgtgccatga
     1621 accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg tgttgctgga
     1681 agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta caaaccaccc
     1741 tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga tcgccatcaa
     1801 tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt ttgaaatgct
     1861 gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg gtggcgatat
     1921 ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg caagtgccgc
     1981 aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg gctttgacga
     2041 gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt ctggactaag
     2101 tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca ccattatctg
     2161 ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc aactggtcaa
     2221 tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc cactacgtgg
     2281 tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt ctgcagagtt
     2341 tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg gacatgctgc
     2401 aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct gcatgggggg
     2461 caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa cgcaattaga
     2521 tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg cacggcatag
     2581 ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg gtgcgcaggc
     2641 ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt taaaacccgc
     2701 cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg cagcactacc
     2761 ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc gcaatgacat
     2821 tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc ccggtggttt
     2881 tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta aggctaattt
     2941 cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta agctggtcat
     3001 ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg atgatcgcta
     3061 tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag ctaaaatttg
     3121 ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg gtaagcgcag
     3181 ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc gctcactggt
     3241 gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc cattaagtgg
     3301 tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat gcctcttctc
     3361 atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta ttgcgcaatt
     3421 tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc ctacagctgt
     3481 aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc agcctgttat
     3541 attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata tttgaatgca
     3601 tgcgaccaca gtgaaaaaca aaatcacgca aagagataac tataaagaaa tcatgtctgt
     3661 aattgtggtt gtcttattac tgacacttac gttgatagcc attttttcgg caattgatca
     3721 gctgagtatt tcagaaatgg gtcgcattgc aagagatctt acacatttca ttatcaatag
     3781 tttgcaagac tggaaataaa cagcaaatta taaatgtgaa atgtttaaaa ggtatcgata
     3841 aaactttatt gttttaagga gataaaatgt cgctcgtttg ttctgttata tttattcatc
     3901 atgctttcaa cgctaacatt ttagataaag attacgcctt ctccgacggc gagatcctga
     3961 tggtagataa cgctgttcgt acacattttg aaccttatga gcggcatttt aaagaaatcg
     4021 gatttaatga aaataccatt aaaaaatatc tacaatgcac taacatccag acagtgacgg
     4081 tgcctgttcc tgcgaagttt ttacgtgctt caaatgtacc gactggattg cttaatgaaa
     4141 tgattgctta tctcaactcg gaagaacgca atcatcataa tttttcagaa cttttgcttt
     4201 tttcttgcct gtctattttt gccgcatgca aaggtttcat tacactatta actaacggtg
     4261 tgctatccgt ttccgggaaa gtgagaaata ttgtcaacat gaagctggcg cacccatgga
     4321 agctgaaaga tatttgtgac tgcctgtaca tcagtgaaag cctgttgaag aaaaaactaa
     4381 agcaagagca aacgacattc tcacagattc tattagatgc cagaatgcag cacgcaaaaa
     4441 atttgatacg cgtagaaggt tcagtcaata aaattgccga acaatgtggt tatgccagta
     4501 catcttattt tatttatgcg ttccgcaaac atttcggcaa cagtccgaag agagtttcta
     4561 aggagtaccg ttgtcaaagg cacacgggta tgaatacggg caacacgatg agtgctttag
     4621 ctatttgatt atttgctaac gagtagtcaa ccacgcacgc tacgtaagaa ttatatgggg
     4681 cagccattcc ctgccccgcg ttgtttttag gcgatatatt tattgaaata aataagtgac
     4741 atccatcaca tatttatgca cttgcataac ctgttgcatg attatttatg atctcaattc
     4801 ggcattttgt cagtaaaatg caataattta ttaaatatca ataaattagt tgtttatcgg
     4861 cgagaaatta cttaatagaa cagaaagtaa tgtcaacgct ttatggactg ttatttccct
     4921 ttttttagct aaatctgcta tctctttatg tgactaactt cacttacatc cacttatttc
     4981 tcttcgtaaa attacttcgg aattaagtac aataagaaga ggaacattta tgaagtctgc
     5041 attaaagaaa agtgtcgtaa gtacctcgat atctttgata ctggcatctg gtatggctgc
     5101 atttgctgct catgcggcag atgatgtaaa gctgaaagca accaaaacaa acgttgcttt
     5161 ctcagacttt ac
//
LOCUS       DAJTUI010000180         5142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTUI010000180 DAJTUI010000000
VERSION     DAJTUI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.180"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..210
                     /locus_tag="OO464_003929"
     CDS             1..210
                     /locus_tag="OO464_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6228876.1"
                     /translation="MFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(382..711)
                     /locus_tag="OO464_003930"
     CDS             complement(382..711)
                     /locus_tag="OO464_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR6228877.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(716..901)
                     /locus_tag="OO464_003931"
     CDS             complement(716..901)
                     /locus_tag="OO464_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6228878.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(898..3537)
                     /locus_tag="OO464_003932"
     CDS             complement(898..3537)
                     /locus_tag="OO464_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6228879.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3745..4734
                     /gene="ldhA"
                     /locus_tag="OO464_003933"
     CDS             3745..4734
                     /gene="ldhA"
                     /locus_tag="OO464_003933"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6228880.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4845..>5142
                     /gene="hslJ"
                     /locus_tag="OO464_003934"
     CDS             4845..>5142
                     /gene="hslJ"
                     /locus_tag="OO464_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR6228881.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLN"
BASE COUNT         1300 a         1318 c         1200 g         1324 t
ORIGIN      
        1 atgtttgccg ataaaggttt ggtagtcgcg caatatattc gtaaccgtcg tctcgatttt
       61 tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac tggcaggcat cggctttcat
      121 tggggatttt ctgaccagag tcatttttca acggtattta agcaacgctt tgggatgacg
      181 ccaggcgagt atcgacgtaa attccgctaa ctaattggcg ttgcatgtac tgcaacgcca
      241 attagttaaa acagatattt gactatgcat ttttcagggc gaaaggcaat ttgacaggag
      301 tttttgaata ttcaccttgt ttcaggaaaa tagatcaatc gctttctcct gtagcgttat
      361 cccgatattg cggttttgct attaaaactt tcgcacccat ttaccgttta tcccttgtac
      421 atattccccc ggtttggccc gagctacgag cttttgcccg gcgagtttcg cgatatcatc
      481 tacagacaca ttattttgct ttgcccgttg ttggtaactc gctttacgtt cggcattaat
      541 ttcggttact aatttctctg tttcagcatc cgttttcagc gcaaccagat aaccataaaa
      601 tgtttcacct acccgcccct gagttctggc ttcatccaga gtcaatgcca ttacattgct
      661 gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca tcgcctcaga
      721 aaagatcgct acgagtttca agcagctctt cgacatcttt gtctgccttg atgatgatct
      781 catgctcaat tttaacgttc atattgatag tgatcggttc cttaggtgca gcgacttcaa
      841 tgcgaggcgt acagccaacc agcataaaag atgacgtcaa cgcagccagt aaaattttca
      901 ttgtttttcc tcacactctt ttccttgcgg acagtcattt cccggcagac gtgcgttctg
      961 ctccaaccat gcctggagat tatcgccaaa gcgtaaactg cgccacagcg taaaaatatt
     1021 ctcttcatga tggtaattaa ggtttaccgt accgctttta ccatcaacgc gactggtacc
     1081 tgtaatgttg gcctgcatgg ttaataaacc gagattatct aagttaattt ttgtcgatga
     1141 ccggctaatt tccatatagc gcaaccagtt aattgctgaa cccgcagtca tattgtcttt
     1201 caccaccgca tctgcggtat ctttatccag ccgcaaggtc attagcccgc tatttgccag
     1261 ccaaccatct ttcactatcc atttttcgtt gtttagccat aacggcaatg caccactaaa
     1321 tgccccggac atggcgaatt gtttcggatt gacggcgcta accagttcgc tggatgagag
     1381 attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt tcatcagcac
     1441 gttaccgcct aacacatcga cgctaacatc agtcaacagc aagggttctt cttcagtcca
     1501 cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat taatcacttc
     1561 ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat cggcaaaacg
     1621 gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg cactaccgcc
     1681 tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag aaaatgcaac
     1741 ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca ccagcggctg
     1801 gaatacagtc agtgattgtt ttggccacca ggcgttaccg cgcagacgaa taccgtccca
     1861 gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt taaagaggaa
     1921 ataggtcgga ttgcgcccat cgacgctaaa ttttaaggtt gatggcggta acacactgcc
     1981 gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg agggatgctg
     2041 tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt ctaccgtcat
     2101 cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa tcgtgctgtc
     2161 atgccactca ccttttcctg cgacatccca ggtggcattc atcggtgtaa aactcccttt
     2221 tccccagtag cgccactgcc agcggccagc gtcagggaga aaatcattcg ccagcccatc
     2281 catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg cctgcaaacg
     2341 tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga tttcatcgat
     2401 gtccagcgaa tcgatgactc ttccctttga acgaagtaac gcgcctggct caaaggtcag
     2461 cgttgggtca gacagacttc cacttagctg cgcaggtaaa cgggcatata aaatgaggtc
     2521 cgcctgtttc gcttcaccgg tcagctgcag aggcagttga ctgttatcca tgcttaattt
     2581 tcctgggcca aaattaagca ccgcgttgcc cttacccgct tgcccctggg tcagcacatt
     2641 cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc cagttgtcga ctttgacacc
     2701 cagtcggcca ctcaaaggaa aaccagcata cagccagctc cagcgaccat cgcttacggt
     2761 caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt tatcccgtgc
     2821 cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca ccagtgacgg
     2881 ttcctgcggc aagtttaacg tcgcagtagc atgcccactt acaggaagtc catccggcac
     2941 gagcggcata gtaaattccc ccaccagttt aaccggcggc tgattttcaa acgcgacgac
     3001 atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt taactttttc
     3061 gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt ttccctgcca
     3121 ttcctgccag ggagaaaaaa tcagtttatc gatattgatc caggtgttag gcagcatgga
     3181 ctgccactgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg gcaatttcgc
     3241 cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc tgggatgtga
     3301 aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac ggagatcggg
     3361 aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac gtgtcccgag
     3421 cggtagccag atgtccgcca gcgtgggaac ccacagcccg agcgtcatca gcagcgtcaa
     3481 cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac ccagcatggg
     3541 tagttaatat cctgatttag cgaaaaatta agcattcaat acgggtattg tggcatgttt
     3601 aaccgttcag ttgaaggttg cgcctacact aagcatagtt gttgatgaat ttttcaatat
     3661 cgccatggct ttcaattata tttgaaattt tgtaaaatat ttttagtagc ttaaatgtga
     3721 ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa acagtacgac
     3781 aagaagtacc tgcaacaggt gaacgagtcc tttggctttg agctggaatt ttttgacttt
     3841 ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg tattttcgta
     3901 aacgatgacg gcagccaccc ggtgctggaa gagctgaaaa agcacggcgt taaatatatc
     3961 gccctgcgct gtgccggttt caataacgtc gaccttgatg cggcaaaaga actggggctg
     4021 aaagtagtcc gtgttccagc ctatgatcca gaggccgttg ctgaacacgc catcggtatg
     4081 atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga cgctaacttc
     4141 tctctggaag gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt tatcggtacc
     4201 ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg tctgctggcg
     4261 ttcgatccgt atccaagtgc agcggcgctg gaactcggtg tggagtatgt cgatctgcca
     4321 accctgttct ctgaatcaga cgttatctct ctgcactgcc cgctgacacc ggaaaactac
     4381 catctgttga acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat cgtcaatacc
     4441 agtcgcggtg cattgattga ttctcaggcg gcaattgaag cgctgaaaaa tcagaaaatt
     4501 ggttcgttgg gtatggacgt ctatgagaac gaacgcgatc tgttctttga agataaatcc
     4561 aacgacgtga tccaggatga cgtattccgt cgcctgtctg cctgccacaa cgtgctgttt
     4621 accgggcacc aggcattcct gacagcagaa gctctgacca gtatttctca gactacgctg
     4681 caaaacttaa gcaatctgga aaaaggtgaa acctgcccga acgaactggt ttaatcttgc
     4741 agctcccctg caatccaggg gagctgattc agataatccc caatgacctt tcattctcta
     4801 ttcttaaaat agtcctgagt cagaaactgt aattgagaac cacaatgaag aaagtagccg
     4861 cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag taatgacaaa attgctgtta
     4921 cgccagaaca gctacagcat catcgctttg tgctggaaag cgtaaacggt aagcccgtga
     4981 ccagcgataa aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt tccggcagca
     5041 tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca gccaaagggc
     5101 tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa tg
//
LOCUS       DAJTUI010000181         5141 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTUI010000181 DAJTUI010000000
VERSION     DAJTUI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.181"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            190..447
                     /locus_tag="OO464_003935"
                     /pseudo
     CDS             190..447
                     /locus_tag="OO464_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO464_003936"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO464_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6228882.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO464_003937"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO464_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6228883.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO464_003938"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO464_003938"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6228884.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="OO464_003939"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="OO464_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3605..4420)
                     /locus_tag="OO464_003940"
     CDS             complement(3605..4420)
                     /locus_tag="OO464_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6228885.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4517..>5141
                     /locus_tag="OO464_003941"
     CDS             4517..>5141
                     /locus_tag="OO464_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6228886.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDL"
BASE COUNT         1370 a         1172 c         1226 g         1373 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctggcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg g
//
LOCUS       DAJTUI010000182         5024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTUI010000182 DAJTUI010000000
VERSION     DAJTUI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.183"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            49..123
                     /gene="tnaC"
                     /locus_tag="OO464_003942"
     CDS             49..123
                     /gene="tnaC"
                     /locus_tag="OO464_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR6228887.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            344..1759
                     /gene="tnaA"
                     /locus_tag="OO464_003943"
     CDS             344..1759
                     /gene="tnaA"
                     /locus_tag="OO464_003943"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR6228888.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1850..3097
                     /gene="tnaB"
                     /locus_tag="OO464_003944"
     CDS             1850..3097
                     /gene="tnaB"
                     /locus_tag="OO464_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR6228889.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3229..4404
                     /gene="mdtL"
                     /locus_tag="OO464_003945"
     CDS             3229..4404
                     /gene="mdtL"
                     /locus_tag="OO464_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6228890.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4379..>5024
                     /gene="yidZ"
                     /locus_tag="OO464_003946"
     CDS             4379..>5024
                     /gene="yidZ"
                     /locus_tag="OO464_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR6228891.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1144 a         1208 c         1255 g         1417 t
ORIGIN      
        1 ttctgagtgt aataatgtag cggcgtgtct ttcgaggata agtgcattat gaatatctta
       61 catgtatgtg tgacctcaaa atggttcaat atcgacaata agattgtcga tcaccgccct
      121 taatttaccc ttctgtagcc atcaccagag ccaaaccgat tagattcaat gtgatcgatt
      181 agtttgctat atcttaattt tgccttttgc aaaggccatc tctcgtttat ttacttattt
      241 tagtaaatga tggtgcttgt atatatacat ggtgaattaa tggatattgc agatgtaata
      301 ttcacaggga tcactgtaat taaaataaat gaaggattat gtaatggaaa actttaaaca
      361 tctccctgaa ccgttccgca ttcgtgttat tgagccagta aaacgtacta ctcgcgctta
      421 tcgtgaagaa gcaattatta aatccggtat gaacccgttc ctgctggata gcgaagatgt
      481 gtttatcgat ttactgaccg acagcggcac cggggcggta acccaaagta tgcaggcagc
      541 gatgatgcgc ggcgacgaag cctacagcgg cagccgcagc tactatgcgt tagccgagtc
      601 agtgaaaaat atctttggtt atcaatatac tattccgact caccagggcc gtggcgcaga
      661 gcaaatctat attccggtac tgattaaaaa acgcgagcag gaaaaaggcc tggatcgcag
      721 caaaatggtg gcattctcta actatttctt tgataccacg cagggccata gccagattaa
      781 cggctgtacc gtgcgtaacg tctatatcaa agaagccttc gatacgggcg tgcgttacga
      841 ctttaaaggc aactttgacc tcgaaggatt agaacgcggt attgaagaag ttggcccgaa
      901 taacgtgccg tatatcgttg caaccatcac cagtaactct gcaggtggtc agccggtttc
      961 actggcaaac ttaaaagcga tgtacagcat cgcgaagaaa tacgatattc cggtggtaat
     1021 ggactccgca cgctttgctg aaaacgccta tttcatcaag cagcgtgaag cagaatacaa
     1081 agactggacc atcgagcaga tcacccgcga aacctacaaa tatgccgata tgctggcgat
     1141 gtccgccaag aaagatgcga tggtgccgat gggcggcttg ctgtgcatga aagacgacag
     1201 cttctttgat gtgtacaccg agtgcagaac cctttgcgtg gtgcaggaag gcttcccgac
     1261 atatggcggc ttggaaggcg gcgcgatgga gcgtctggcg gtaggtctgt atgacggcat
     1321 gaatctcgac tggctggctt atcgtatcgc gcaggtacag tatctggtcg atggtctgga
     1381 agagattggc gttgtctgcc agcaggcggg cggtcacgcg gcattcgttg atgccggtaa
     1441 actgctgccg catatcccgg cagaccagtt cccggcacag gcgctggcgt gcgagctgta
     1501 taaagtcgcc ggtatccgtg cggtagaaat tggctctttc ctgttaggcc gcgatccgaa
     1561 aaccggtaaa caactgccat gcccggctga actgctgcgt ttaaccattc cgcgcgcaac
     1621 atatactcaa acacatatgg acttcattat tgaagccttt aaacatgtaa aagagaacgc
     1681 ggcgaatatt aaaggattaa cctttaccta cgaaccaaaa gtattgcgtc acttcaccgc
     1741 aaaactgaaa gaagtttaat taatactaca gagtggctat aaggatgtta gccactctct
     1801 taccctacat cctcaataac aaaaatagcc ttcctctaaa ggtggcatca tgactgatca
     1861 agctgaaaaa aagcactctg cattttgggg tgttatggtt atagcaggta cagtaattgg
     1921 tggaggtatg tttgctttac ctgttgatct tgccggtgcc tggtttttct ggggtgcctt
     1981 tatccttatc attgcctggt tttcaatgct tcattccggg ttattgttat tagaagcaaa
     2041 tttaaattat cctgtcggct ccagttttaa caccatcacc aaagatttaa taggtaacac
     2101 ctggaacatt atcagcggta ttaccgttgc cttcgttctc tatatcctca cttatgccta
     2161 tatctctgct aatggtgcga tcattagtga aacgatatca atgaatttgg gctatcacgc
     2221 taatccacgt attgtcggga tctgcacagc cattttcgtt gccagcgtat tgtggataag
     2281 ttcgttagcc gccagtcgca ttacctcatt gttcctcggg ctgaagatta tctcctttgt
     2341 gatcgtgttt ggttctttct tcttccaggt cgattactcc atcctgcgcg atgccaccag
     2401 caccactgcg ggaacgtctt acttcccgta tatctttatg gctttgccgg tgtgtctggc
     2461 gtcatttggt ttccacggca atattcccag cctgattatt tgctatggca aacgcaaaga
     2521 taagttaatc aaaagcgtgg tatttggttc gctgctggcg ctggtgattt atctcttctg
     2581 gctctattgc acgatgggga atattccgcg cgaaagcttt aaggcgataa tctcctcagg
     2641 cggcaacatt gattcgctgg tgaaatcgtt cctcggcacc aaacagcacg gcattatcga
     2701 gttttgcctg ctggtgttct ctaacttagc tgttgccagt tcgttctttg gtgtcacgct
     2761 ggggttgttc gattatctgg cggacctgtt taagattgat aactcccacg gcgggcgttt
     2821 caaaaccgtg ctgttaacct tcctgccacc tgcgttgttg tatctgatct tcccgaacgg
     2881 ctttatttac gggatcggcg gtgccgggct gtgcgccacc atctgggcgg tcattattcc
     2941 cgcagtgctt gcaatcaaag ctcgcaagaa gtttcccaat cagatgttca cggtctgggg
     3001 cggcaatctt attccggcga ttgtcattct ctttggtata accgtgattt tgtgctggtt
     3061 cggcaacgtc tttaacgtgt tacctaaatt tggctaaatc cttcaagaag ccagccattc
     3121 gctggcttct tgcctctcag gaaatcactt atgtccaaat ggcaactcgc cttatcctcc
     3181 ttcaccacgt atgctttgcg tcaccttact atcaggacgc tttagcccat gtcccgcttt
     3241 ttgatttgta gttttgccct ggttttactt tatcccgccg ggattgatat gtacctcgtt
     3301 ggtttaccgc gcatcgccgc cgatctcaat gccagcgaag cgcagttgca tattgcgttc
     3361 tccgtatatc tggcggggat ggcagctgcg atgctatttg ccggtaaagt ggccgatcgt
     3421 tcagggagaa agccggtcgc catacccggc gcggcgctat ttattattgc ctcggtgttc
     3481 tgttcactgg ctgaaaccag cacgttattt cttgcaggcc gatttctaca ggggttgggc
     3541 gcaggctgtt gttacgtggt ggcgttcgcc attttgcgcg acacgctgga tgatcgacgt
     3601 cgggctaaag tgctgtcatt actcaacggt attacctgca tcattccggt gttagcgcca
     3661 gtgctcggac atctgattat gcttaaattc ccgtggcaga gtctgttctg ggcgatggca
     3721 atgatgggca tcgcggtact gatgttgtct ttgtttattt taaaagaaac gcgcccagcg
     3781 tcccccgctg cttcggacaa accacgagaa aatagcgagt cgctgcttaa tcgttttttc
     3841 ctcagccgtg ttgttatcac caccctcagc gtttcggtga tcctcacttt cgtcaataca
     3901 tcgccggtat tgctgatgga aatcatgggg tttgagcgcg gagaatacgc caccattatg
     3961 gcgctgaccg ctggcgtcag catgaccttt tcattctcga cgccatttgc gctgggaatt
     4021 tttaagccac gtacgttgat gatcacctcg caggtgttat tccttgcggc ggggatcacc
     4081 cttgccgttt caccttccca tgcggtttct ctgtttggta tcacgctgat ttgcgccggt
     4141 ttctcggtag gttttggcgt agcgatgagt caggcgttag gaccattttc attacgcgcg
     4201 ggcgtagcca gctcgacctt aggtattgcg caggtttgcg gttcgtcact gtggatttgg
     4261 ctggccgcgg tggttggtat cggcgcatgg aatatgctga tcgggattct gattgcctgt
     4321 agcatagtga gcctgttgct gattatgttc gtcgcgcctg gacgccccgt tgccgctcat
     4381 gaagaaatcc atcaccacgc ttgatctcaa tctgctgctc tgtctgcaac tgctgatgca
     4441 ggagcgcagc gtgaccaaag cggcgaagcg gatgaacgtg accccttcag cggtgagtaa
     4501 gtcgctggca aagttaagag cgtggtttga cgacccgctc tttgtgaact caccgctggg
     4561 gctgtcgccc acacagctaa tggtcagcat ggagcaaaat ctggcggaat ggatgcaaat
     4621 gagcaatctg ctgctggata aaccgcacca ccagacaccg cgcggcctga agtttgagtt
     4681 ggcggcggaa tcgccgctga tgatgatcat gcttaatgcg ctgtcgaaac ggatctacca
     4741 acgttacccg caggcgacca tcaaactacg taactgggat tacgattcct tagatgccat
     4801 tactcgtggt gaagtggata ttggtttttc cggtcgcgaa agccatcctc gctcgcggga
     4861 gctgttaagc tcgctaccgt tagccattga ttatgaagtg ctgtttagtg atgtaccctg
     4921 cgtctggtta cgcaaagatc atccggcact gcatgaaacg tggaatctgg acaccttttt
     4981 acgttatccg catatcagca tttgctggga acagagcgac acct
//
LOCUS       DAJTUI010000183         4996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTUI010000183 DAJTUI010000000
VERSION     DAJTUI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.184"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(1..279)
                     /locus_tag="OO464_003947"
     CDS             complement(1..279)
                     /locus_tag="OO464_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228892.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="OO464_003948"
     CDS             220..405
                     /locus_tag="OO464_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228893.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO464_003949"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO464_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6228894.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="OO464_003950"
     CDS             complement(1254..1670)
                     /locus_tag="OO464_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228895.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="OO464_003951"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="OO464_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2862
                     /locus_tag="OO464_003952"
     CDS             1837..2862
                     /locus_tag="OO464_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6228896.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2915..3004)
                     /locus_tag="OO464_003953"
     tRNA            complement(2915..3004)
                     /locus_tag="OO464_003953"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="OO464_003954"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="OO464_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6228897.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="OO464_003955"
     tRNA            3996..4071
                     /locus_tag="OO464_003955"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..4996
                     /locus_tag="OO464_003956"
                     /pseudo
     CDS             4233..4996
                     /locus_tag="OO464_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
BASE COUNT         1336 a         1179 c         1147 g         1334 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaa
//
LOCUS       DAJTUI010000184         4953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTUI010000184 DAJTUI010000000
VERSION     DAJTUI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.185"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(176..760)
                     /gene="kptA"
                     /locus_tag="OO464_003957"
     CDS             complement(176..760)
                     /gene="kptA"
                     /locus_tag="OO464_003957"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR6228898.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1010..1693
                     /locus_tag="OO464_003958"
     CDS             1010..1693
                     /locus_tag="OO464_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR6228899.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1690..2151
                     /locus_tag="OO464_003959"
     CDS             1690..2151
                     /locus_tag="OO464_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR6228900.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2164..3336
                     /gene="iadA"
                     /locus_tag="OO464_003960"
     CDS             2164..3336
                     /gene="iadA"
                     /locus_tag="OO464_003960"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6228901.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3401..4312
                     /gene="hypT"
                     /locus_tag="OO464_003961"
                     /gene_synonym="qseD"
     CDS             3401..4312
                     /gene="hypT"
                     /locus_tag="OO464_003961"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR6228902.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4305..4697)
                     /gene="iraD"
                     /locus_tag="OO464_003962"
     CDS             complement(4305..4697)
                     /gene="iraD"
                     /locus_tag="OO464_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR6228903.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4694..4777)
                     /gene="idlP"
                     /locus_tag="OO464_003963"
     CDS             complement(4694..4777)
                     /gene="idlP"
                     /locus_tag="OO464_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR6228904.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1170 a         1302 c         1233 g         1248 t
ORIGIN      
        1 aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt aatttcagca
       61 gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa caaacgctac
      121 attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat tgaatttatt
      181 gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca accgttgatg
      241 tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc atttcctgtg
      301 ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt gccgttgctt
      361 catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt ttttttatct
      421 cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt ttttcggcaa
      481 aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac ctaccatcac
      541 tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc aatgcacggg
      601 ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc gcccatcctt
      661 cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa cttaaaaact
      721 tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg tccctggatt
      781 ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg caactcaatc
      841 ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac gcatagccag
      901 aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag ttccaggaat
      961 tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta tgacacaaca
     1021 aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag ggtatctggc
     1081 tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca gctggtggcg
     1141 cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg cgaatggagc
     1201 caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc tctttgcgct
     1261 ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg atggtcttgg
     1321 cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt tgggcattcc
     1381 agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg ccgccggtat
     1441 gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga ttttcgccgc
     1501 atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg tcgcggtgtt
     1561 tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt ttaaatttgt
     1621 cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc caacgcaagg
     1681 agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat cgatggcgcg
     1741 cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat ggcgtttgtc
     1801 atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat ctgtgaaccc
     1861 gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc cgcattaatg
     1921 agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc attaaccggc
     1981 cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt gcaaaatgtc
     2041 ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat cattacggtc
     2101 tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta acaaggagtt
     2161 accatgattg attataccgc agccggtttt accctgctgc agggagcgca tttgtatgcg
     2221 ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat cgccgttgcc
     2281 agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag cgggcagatc
     2341 ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg cgaagcaggt
     2401 cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg cgtcacgtca
     2461 gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct gctcgccaag
     2521 acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc ttatcatgtc
     2581 ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga tcgtgtgatt
     2641 ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta tcacctggct
     2701 aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg cgtcaccgtg
     2761 ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct ggaaaactgc
     2821 gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc gttgtttgag
     2881 caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag cattgacgaa
     2941 ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc gctggcacgc
     3001 gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga agggaattta
     3061 acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt gctggtcaaa
     3121 gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt agccggtttc
     3181 cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt actggtcatg
     3241 acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt caaagacggc
     3301 aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg ccctaatgga
     3361 cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact gtggtgcggt
     3421 tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa aatgccgcaa
     3481 tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc gccgcatccg
     3541 tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc cactgcaact
     3601 ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac agttagaaag
     3661 caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca agatagccgc
     3721 tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc cgccgctctt
     3781 tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc gtgaagggca
     3841 aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt ttgaccacat
     3901 tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg gagaagcact
     3961 ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact cctacatggt
     4021 gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca ccttttttgt
     4081 ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga ttgcctggct
     4141 gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc tgaatcggga
     4201 cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca tgaatccagt
     4261 tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct gacattctca
     4321 agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag cggttcatcg
     4381 tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc ttctttcaaa
     4441 ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg cagctgacgg
     4501 caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc gggcatgcct
     4561 gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag gagatcgctg
     4621 cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa aacagcctga
     4681 agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg gcttgccctg
     4741 aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt tgttcattac
     4801 atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac cttacttgcc
     4861 tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat catcaaactc
     4921 aaaaaaaaaa cgctagtata ttctgaatat tcc
//
LOCUS       DAJTUI010000185         4787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTUI010000185 DAJTUI010000000
VERSION     DAJTUI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.186"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(281..1138)
                     /gene="sitD"
                     /locus_tag="OO464_003964"
     CDS             complement(281..1138)
                     /gene="sitD"
                     /locus_tag="OO464_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6228905.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1135..1992)
                     /gene="sitC"
                     /locus_tag="OO464_003965"
     CDS             complement(1135..1992)
                     /gene="sitC"
                     /locus_tag="OO464_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6228906.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1989..2816)
                     /gene="sitB"
                     /locus_tag="OO464_003966"
     CDS             complement(1989..2816)
                     /gene="sitB"
                     /locus_tag="OO464_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6228907.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2816..3730)
                     /gene="sitA"
                     /locus_tag="OO464_003967"
     CDS             complement(2816..3730)
                     /gene="sitA"
                     /locus_tag="OO464_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6228908.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4162..>4787)
                     /locus_tag="OO464_003968"
     CDS             complement(4162..>4787)
                     /locus_tag="OO464_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6228909.1"
                     /translation="TEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVDFEKG
                     IVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFYHREY
                     GRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHTFACW
                     LLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1266 a         1173 c         1131 g         1217 t
ORIGIN      
        1 atcgccgtca gcgggagctg aaggaagagc tgaatagtct gaaggatttg aattcggtta
       61 ggaattttat cgtggaataa cagaagctgc ggcacgtcgt atgcaggaac gtgccgcggt
      121 tggctggtaa actttcgata gtgcgagtat tgaatgattt ctaggcgtta tcgattttac
      181 gtattttttg catgatagaa ttcgtacctc ctcccaacga ccttccatgg ctttccggac
      241 ttctgtaggc tggcggacga tgctgtcctc ggatgttgtg ctaattgggt gataacagat
      301 cctgtgtatg ggcgtttccc tttgtgcgtg cgttgatgct ggtaacggta aacgtcatga
      361 tgaacacgac cgcgaataag acgacaatcg tcggagctgg tgcgctgtcg ataaaaaagg
      421 acagccagac accagtcatt gacactatta ccgatatgcc agtcgccagt aggagcgcaa
      481 tatgaaagcg ttgtgtcagg agtactgcaa tggcgccagg cgcaatgagc agagaaatag
      541 agagaatgat acctactgct ttcagcgtcg ccacaatggt cagggaaacc atgcacagca
      601 gcccataatg cagccatctc gtatgcaaac cgcttacctg cgcctgttga taatcaaagc
      661 tgaacagcaa aaaatcccgc cattttacgc taataacaag tgtaaccagc ccggcaataa
      721 tcaccgtctg gattatgtcg ccgatggtta tcccgagcat gtcgccgaaa agaatatggt
      781 caagatgtac gtctggttta attgcaatat acaagatcag acctgcggca aacatgccag
      841 aaaaaacaat ccccataact gtatcttgct tgattcggct gttgtctttc aggtatcccg
      901 tcgccaccgc gcagaatact ccggcgacga atgcccctgt agccagaggt aaccccagaa
      961 tccaggtaag cacgatgcca ggaaaaaccg catgactcat cgcgtcaccc atcagtgccc
     1021 aacctttcag gaccagaaag accgacagca gcgcacaggg tatggaaact accagtgaaa
     1081 tcagcagtgc gtgactcata aaggtgaact gcagtggctc cagtaataat gccatcatga
     1141 gcgatcctcc agcgctttat gcgcacgccg acgatttgcc aaaagtccat gggtgggcgc
     1201 aaatacaaat gctaaaagaa acagcagtgt ctgcgccacc acgatgattc cacctgtcgc
     1261 gccgtccaga aagtaactga cccacgcgcc gaggaaactg gttacactgc caatagttac
     1321 agcaattatg agcaggcggg gaaaacgatc ggttaacaac catgctgttg caccgggagt
     1381 caccaccaga cagatcacca gaaaggcacc aaccgtttgc agcgccgcaa cggtcgaaac
     1441 cgacagcagg gtgaagaaga ggatttttaa tctgcccgga tgtaatccaa tagcacgggc
     1501 gtgattctcg tcgaaaaagg tcaccataag atctttccat ttgaaaaaaa gcacgatgat
     1561 cgagagaatg ccaatgatag tgagttgcag gatatccgcc ggatcgattg ccagaatatt
     1621 gcccaggacg atggtctgaa tgttcacgga tgtcgggttc aatgacacca taaacagccc
     1681 caggccaaaa aaggacgaga agatcaggcc gatgatggca tcttctttta agcgggtgcg
     1741 ttggttaaga aacagcatgc tgcccgctgc cagtccgccg gaaaagaagg ctcccagaga
     1801 aaacggaagc cccagcatat atgcccccgc aacacccggg acaatagagt gcgacagcgc
     1861 atcgccaatc agcgaccagc cttttagcat cagatagcat gacaggaaag cgcagagacc
     1921 gccgaccatt gccgagaccc acatcgcatt gagcatatat tcatagctga agggttccag
     1981 tagcacgttc atcttgcttc cctctgcacc gctgacggtc gatgtgccac aaaagggcgt
     2041 tcatcgtcag taataatgct ttcttccgag ccgttaagtg tgacatggcg cagtacgccg
     2101 ctaaaagcca gctccaggtt ctcggctgta aaagtggtgt ctgtcggccc gcttgccagg
     2161 acggtgcctt tgaccatgac cgtataatcg caaaatgtcg tgactgagcc gagattgtgc
     2221 gttgagacaa gcatcgtttt gccttccgcg cgtaactccc tcaacaggct gatgattttc
     2281 gcttcggttt tcacatcaac gccggtaaaa ggttcatcca gcaggatcac atcaccctgc
     2341 tgtgcaattg ctctcgccag aaagacgcgt ttcttttgtc caccggaaag ctcgccgatt
     2401 tgacgatggc gaaaatccac catatcgaca cgttccagcg catcggtgac aatctgacga
     2461 tccctctttt tggctatacg caacataccc atatgcccat agcggcccat catcaccaca
     2521 tcttccacca gtacaggaaa tgaccagtca acctcttctg actgagggac gtaggcaacc
     2581 aggtttttct gtagcgcctg tcgcgtggga atacccagaa cagatatctt tccgctggtc
     2641 agacgcacaa atcccataat cgctttaaac aacgtcgatt ttccggaacc gtttacccca
     2701 accagagcgg caatagatcc acttggtact gtgaaggatg catcgcgcag tgctgtgtgc
     2761 ccgttacgcc aggtgacagt aacatcatta acgacaatgc ctgcagattg catcattatt
     2821 tctccctttt cccggcttta attccctgta ccagcgtact ggtagtaacc ttaagaaggt
     2881 cgatatacgt aggtaccggg ccgttttctg tgctcaggga atcgacatag agcacaccgc
     2941 cgtagtgtgc gccggtttca cgcgcaacct gacgcgctgg tttatcggaa atcgtactct
     3001 cgctaaagac tgccgggata tgatttttct taactatatc aacaacctta cgtacctgct
     3061 gcggtgttcc ttgttgatcg gcattaatcg gccacagata aagctctttt agccccaaat
     3121 cgcgtgcgag ataagaaaaa gccccttcac tggtgaccat ccatcgctga ttctcaggga
     3181 gttccgtaat ctgcttacgc aggggggcaa gggtttgggt aatcttggct ttataagtat
     3241 cggcattacg ttggtaggtt tgtgcatttg ccggatcgta ttttatcaac gcatcacgaa
     3301 tattatcgac gtaaatcaga gcattatctg gcgacatcca tgcatgggga ttaggtttgc
     3361 cctcataggg tccttcggtg atccctactg gcgtcacacc cgaagagaca attacttctg
     3421 gaaccccatt gagatgctgg taaaagcgtt ggaaccacaa ttccagattc ataccattgg
     3481 cgagaatcag ttgtgccccc tgcgcacgtt taatatcgcc aggggtaggc tgatactcat
     3541 gaatttctgc accaggcttg gttatggatg agacttctgc agcatctcca gccacgtttt
     3601 tggccatatc cgcgatgatg gtaaatgttg taatgacctt gaatttttca gttgcacttg
     3661 cctgaaatgc gatcgagcag gtgagggcga ggcaccccaa gagcatggtt acttttttta
     3721 ttgagtgcat aatttagtac ctatagtgag caaaattaga acaaacatag caacggctat
     3781 gttttatagc ataacctata caaaatgaaa ataattatca ttaacagctt tgaggcctaa
     3841 aacagggatt aggttgtttc ctgctggcga catgatattg aggaaaaaag tagagcagct
     3901 attaggcttt gaaatgactt tatgaggaaa attgttgatg gcaaaaatgg ggcaaaaaag
     3961 agtgggttat cgtagcttat tgttgtcgct gataatgttt aacatattga aaaataaata
     4021 aaatacttat gagtcagata gttgtgattt ttgcccttac ttgttcaggt tgtattgttc
     4081 tttctttgta atttgttgat tttcttgcat tatttcagtt ctctggtact aaatggggca
     4141 aattgggggc aaactttgca actacgataa ccgcgcattc aacatggcta tctgttcgtc
     4201 gttcatgtca tcaatccaca taccgtaaat ttcatacacc atttgcgcag tttcatgccc
     4261 catctggctg gctataaatg ccgggttcgc tcctgccgtc aacagccagc aggcaaaagt
     4321 atgtcgcgta tggtacggat tacggcggcg aataccagca cgttttactg ctgcattcca
     4381 tcttgtaccc aaactgctta ccgagtaata aggtttctgt ttttcgttac acaccctggg
     4441 catgaaaaca aaatgcagtt tttgtttttc agttctgccg tactcccgat gataaaaagt
     4501 gatttcgctt ttgcgatgat gcccggtcag tttgtattgc tccttcagtg cttcaagagc
     4561 cggctgcagt aatgctaccg tccggatccc ggcatttgtt tttgggggac cgaacatatc
     4621 aagtatcgtc aggtttcttc tgacattcac aattcccttc tcgaaatcca catcctccca
     4681 cgccagagct gccagttccc cgtgacgaag cccggagtaa acggcaaatt tccacaagtt
     4741 ttggctctgt cctttttcac tttccattaa tgcattgaat tctgttt
//
LOCUS       DAJTUI010000186         4761 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTUI010000186 DAJTUI010000000
VERSION     DAJTUI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.187"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..1303)
                     /locus_tag="OO464_003969"
     CDS             complement(<1..1303)
                     /locus_tag="OO464_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR6228910.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSD"
     gene            complement(1319..2203)
                     /locus_tag="OO464_003970"
     CDS             complement(1319..2203)
                     /locus_tag="OO464_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6228911.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2214..2819)
                     /locus_tag="OO464_003971"
     CDS             complement(2214..2819)
                     /locus_tag="OO464_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6228912.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(2990..>4761)
                     /locus_tag="OO464_003972"
     CDS             complement(2990..>4761)
                     /locus_tag="OO464_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HCR6228913.1"
                     /translation="VRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIFKRSNI
                     RMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMRTLPRL
                     KGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDREPLSVE
                     IMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPESIIDGY
                     IRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRMTPQES
                     DKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVIHSDAA
                     LQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETSADMIL
                     SELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPRNDIWA
                     KFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLTFFKFG
                     ALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPELDLVR
                     QLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1380 a         1182 c          994 g         1205 t
ORIGIN      
        1 tatcgcttac ggcaggctcg tttgccagcg catccagtac atagcgtcca tgccctgcgg
       61 caatgtcaac cacgcggacg gctaaacctt tggcgtggag atcggcaacg gcttgtttaa
      121 tcaatatttg caaatgggtt ttgcgctggc gaataccgcg ccatccaaca ctgttcaggt
      181 agattttgtc gattaaacgc ccgaatgcgt tactgccttg cggttgattt tgatagacat
      241 aatccaggga actgcctgaa tcaaagccgg tactcattcc gagatgcagg cccgcagagt
      301 gcgcaccgaa caatttcatc gcctttcgca taaagcgata cgctaaatca accggcgata
      361 atggcacggg tccaccagaa aggagccgcc atcgatccgc tgatggtcct gtgcggtctt
      421 catgctgata atcaaatttt tgcgatttgt tagcatataa gcggcagata aagctttgca
      481 ttttttcaaa tgcctgcgcc ctgttctctt cacccaatgt gtcgtgataa aagcctggca
      541 gcagatgcag ctctttcaga gggctacgta atctctgata aaaatcaatc tgcggttggc
      601 gatgcaccac atagtcatca cctgatatca gaagttgcgt ggggagcgta atcgccgccg
      661 catcgctaac aatacgttca gacgttttat agagatcgag caagatattg acggcaatcg
      721 cccgtgtgat cagcggatca ttattgaaac tcgccacccg ttgccgatcg tgggtcaaat
      781 agcgcccttt cacataggaa ttaataaaaa atagaccacg cagacgatgc cataacgcca
      841 gcgcaggacg tgccagcggc acatacaatt taaccttaaa ggccggagaa gccagcacca
      901 gcccacgaat tgcaggtgca taatcatgaa cccatgtggc aacaagcact gcgccgacgc
      961 tttgcgcgat cactaccacc tcttccagtc cgacctggct gtcgctggca gcaaaacgga
     1021 caaattcatc gacatcctgc actgagcgca taagagacgg gctataacca cgcggccccg
     1081 aattttttcc atgcccccgg gcatcccagg cataaaaagc agtatcaggc atcgccagtt
     1141 catcaacgag atgttgtaga cgaccagaat gttcatgccc gcgatgaaat aagacgatga
     1201 cctttttcgc ccctggctgt aaagtgggcc agtgccgata atacaatgct gtattatcac
     1261 tggtggtaaa aaaatgttcc ccagggatgc gtgaattttc cattttctct attttccttc
     1321 agtaacagca gtagcgaata aaataaaaaa ataccggagc ggtgaaaatt aatgagtcaa
     1381 tcctgtcaag gagtccgcca tgtcctagta atagttttcc actatcttta acaccaatat
     1441 ctcgtttgat ggctgacatc actacatcgc cgcaaaaacc actaatacca attaacaaac
     1501 ccgctaataa tgcctgtaat gtatttagcg gtgtcagcaa cggtccaata atcagtgacg
     1561 caatcatggt ggtgattacg ccccccagca gaccttccag cgtttttccc gggctgactt
     1621 tggggaccac ttttcttctg ccgcaggatt tcccccataa atactgtgca atatcgtttg
     1681 actcggttaa cgccaaaaga aataacacca gtaacgcccc gccctggata tttatggttg
     1741 gcaacattaa tagccaggcg gcatgactca aggcaaaaac ggtcattatc catccccaaa
     1801 aaatggcgct cacggtatgc tgaaaaccgg aggggtcacc cacaaaaact cgccaggtgg
     1861 ctaatatcag aaacccagtc aaaggtataa ataacagaaa cagctcaaag caattaaaac
     1921 cgataagcaa atagttaaga ggaataaccc aatataacca acgcgaaaaa tgtacggata
     1981 taagcgtaca atattctttc aatgccagaa aacttatcag cgtaaaaaac gtcaacgtca
     2041 tccagcgtgg gcctgaaatc accagtgaaa aacaaatgac gatgccccac caggtacgaa
     2101 tacgtaatgt aacttcatcc ccttttctct caggtaaccg ccatagcaga aaacggttta
     2161 tcagcttgct aaaattaaca acgcgaaaag tgttgccaga gatttttcca gcattatctt
     2221 tcagcgctca taagaagcac actccgacaa cgattaatcg cagtacagat aagcagaatt
     2281 gaagcaatgc tccataaaag attattccat tgcatccact gtggataaat ggcaacggcc
     2341 agaccccaca gaccaaatat taacgcgcga tcacttttgc caaatggacc ggcataactg
     2401 cgaacaccgt taatcgtctg cgcgagtaaa ccgcaaaact cggtcaatat ggtgcaaaat
     2461 aacatgagta tcacgagtga tgcgttactt tccggtaaaa ataaaaacgg taaatagagc
     2521 gcaatatcgg aaataacatc gccagtctca ttcaaaatcg cccccagacg tgtttgctga
     2581 ttgcactcac gcgccaacat gccatccagc gcattgagcg ccatacggat aaaaagcacg
     2641 atgggcaata gcaaaaagag gatgggttgt gccaccaaca ccaacagcaa tccggtaaaa
     2701 agagaaagcg ccagtgccgt aagagtgatg tgattcgctg taacgtggtg cttataaagc
     2761 caaaacatcg tcggccttaa cagcgactga aagagcggtt ttatctggta gagcgtcatt
     2821 atcaatcctt gataattcgg tttgcgctat ggtgcatcaa ttcccctgac ggagatatcg
     2881 caacacgccg cttcattaaa aattcagaat atatcaaggt attgggggct cactgtcagg
     2941 aatgattatc cccaataaat caagtagtta aaaattatta cgctgttatt caatacaact
     3001 catcaagttt cctcatcgct tgttcccgta ttgcgcgcgt cttaccgtta agcgccagct
     3061 ggcggactaa atccagttca gggttaacaa gctctaacgc cagcaaagga tcacgtttca
     3121 gcctcgcctt caatacatca actggaaagc ggggattatt catcgccaca atccaccact
     3181 cgggatcaat ttctgccgcc agtgccgcag agggggtgtg gcggttgcta agtgcgccga
     3241 acttgaaaaa tgtgagattg cttttcgcca gtgtactcca ccatatacga tccggttgtt
     3301 ttgccagtac atccagttgt gctaacgcat cggggtcgag tgcataataa acacgtaatg
     3361 cttcatcaac ctgcgcacgg gagctatttt cctgcctcag aaaaaacttc gcccaaatat
     3421 cattgcgtgg cgtgttttgc tgtaattcct tgagaacaac agcgggtaaa ttatcccaac
     3481 taactaatgc actctctaaa aagtcccagt cttccaaacg tatcagggcg gcggcattta
     3541 atagcatctg gtcatcaact tcgccgtggc taatcagctc agacaaaatc atatcagcgc
     3601 tggtttcata tgcatctgaa tctgctttcg gattattttt ccgatgcgat aaagcacgtt
     3661 gccaaatttt ttgtttcatc gacgtgctca gatactgatt tttcgccaga ttactccacc
     3721 cctcaggatc gttgaaacgt gtcaataacc gttgtaaagc agcgtcagag tgaattacct
     3781 gcatatcccg acagcaaaag taggcagggg attttttctc catcctttta acaaaattaa
     3841 actgtaagtt atctggcaat gctaaaaata acacacgtat tagatcatcg tcattctcga
     3901 atttttggta taaataggct gcaactttgt cactctcttg tggcgtcatc cgcaataatt
     3961 gaccatggtg tttcatctct gccagcttga acgctaagct tgtgacaaca ttgtttgatg
     4021 aatcgttcgc caactgcatt gcctgattgt aagtgagttg tcctccagca ccgacctgac
     4081 gaatagagga atcatcactg cggatatagc catcaataat ggattcgggc aataaccacg
     4141 catctttata aagctcctga atgatcatat cccaatctct tttcgccaga gtcatgacga
     4201 tatccagagg gagattttca cgtagcgcaa gattcaacgg accgacacca tcaggtagac
     4261 gactcattcg cgtcattatt tcgacagaga gcggttcacg gtcgagcaac cccagaacaa
     4321 caggctggta acccaattgc tgatcggtca actccagaat ctcattgtca ggcatatatc
     4381 tcgccatact ttcaatcgtt tgttcatcat tgctattaat aagttgtttg cgtaggttag
     4441 ctgaaattcc tttaagcctt gggagagttc gcattatggc aagatcaaat gtcgcaatat
     4501 ctcgtccttc aaatgcagca acgttgcctt catccagcat ttgcaattcc tgcactaact
     4561 caggagaagg cttagggcga aacagcgctt cttttttcat ctgattacag acattccctg
     4621 ccatgcgaat attgcttcgc ttaaaaattt ctttcagagc atcttcatca tttattagac
     4681 cccaataata tgcatcagga tttattatca tcagcgcttt tttgacattt tcagtgtcat
     4741 ttttgtcact cgcacggacc a
//
LOCUS       DAJTUI010000187         4734 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTUI010000187 DAJTUI010000000
VERSION     DAJTUI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.188"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            223..4341
                     /gene="pic"
                     /locus_tag="OO464_003973"
     CDS             223..4341
                     /gene="pic"
                     /locus_tag="OO464_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCR6228914.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGDTVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4492..>4734)
                     /locus_tag="OO464_003974"
     CDS             complement(4492..>4734)
                     /locus_tag="OO464_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR6228915.1"
                     /translation="AYPTDIRKVIYTTNAIESLNSVIRHAIKKRKVFPTDDSVKKVVW
                     LAIQSASQKWTMPLKDWRMAMSRFIIEFGDRLDGHF"
BASE COUNT         1272 a         1074 c         1251 g         1137 t
ORIGIN      
        1 ggcagaagca gaaaaagctt attatgcttc catcggaaat gatgatctgg cagcctgagt
       61 tcacagataa aacactctcc aggaaacccg gggcggttca gttcacaaaa acacattaat
      121 gcagtaacta tattttcctt tctggtgata acgtcgggtt atcattagct tcttcagcta
      181 ttttactttt atatcccttg taaacatcat ggagaatcca tagtgaataa agtttattct
      241 cttaaatatt gccccgtcac cggggggctt attgctgtct ctgaacttgc ccgcagggta
      301 ataaaaaaga catgccgaag attaacgcat attcttctgg ctggcattcc agcaatctgt
      361 ctgtgttact ctcagatatc ccaggcgggt attgtccgtt ccgatattgc ctatcagatt
      421 tatcgtgatt tcgccgaaaa caaagggctt tttgtacctg gtgccaatga tattccggta
      481 tatgataagg acggaaaact tgtgggaaga ctgggtaaag ccccaatggc cgatttcagc
      541 agtgtgagct caaatggcgt tgctacgctt gtatcgcctc agtatatcgt cagcgtaaag
      601 cataacggag gatatcggag tgtgagcttt ggtaatggga aaaatacata ttcccttgtt
      661 gaccgtaata accacccttc tattgacttc catgctccac gtctgaataa actggttaca
      721 gaagttattc cctcagcggt aacatcagaa ggaaccaaag ccaatgctta taaatacact
      781 gaacgttaca ccgcttttta tcgggtgggt agtggtacgc agtacactaa ggacaaggac
      841 ggaaatttag ttaaggttgc cggtggatat gcttttaaaa caggaggaac cacaggagtt
      901 cctctgatat ctgatgcaac aatagtctct aatcccgggc aaacttataa tcctgtaaac
      961 ggccctttac ctgactatgg agcccctggg gatagtggtt ctcctttgtt tgcttatgat
     1021 aaacaacaaa aaaaatgggt tattgttgct gtattaagag catatgcagg tattaatggt
     1081 gctacgaact ggtggaatgt cataccaaca gattatctga accaggttat gcaggacgat
     1141 ttcgatgccc ccgtagactt tgtttccgga ctgggccccc tgaactggac atacgacaaa
     1201 acatcaggca caggtaccct gagccagggc agtaaaaact ggaccatgca cgggcagaaa
     1261 gacaatgacc tcaatgccgg taaaaatctg gtattcagcg ggcagaatgg tgcaattatc
     1321 ctgaaagaca gtgtgactca gggtgccggt tatctcgaat ttaaagacag ttacaccgta
     1381 tctgctgaat ccggaaaaac atggacgggt gccggcatta ttactgacaa ggggacgaat
     1441 gtaacctgga aggtcaacgg cgttgccggt gacaacttgc ataagctggg ggaaggaacc
     1501 ctgaccataa acggaacagg tgtaaacccg ggaggactga aaacgggaga cggtatcgtt
     1561 gtacttaacc agcaggcaga cactgcaggt aatatccagg ccttcagttc agtgaacctc
     1621 gccagcggac gtccgaccgt ggtgctcggg gatgcccgtc aggtcaatcc ggataacatt
     1681 tcatggggat accggggagg taagcttgac cttaatggta atgccgttac cttcacccga
     1741 ctgcaggctg ctgattacgg ggcggtgatt acaaataatg cacagcaaaa atcccagctt
     1801 ttactggatc ttaaggctca ggatacaaat gtcagtgaac cgacgattgg aaatatatcc
     1861 ccctttggtg gtaccggcac tccaggaaac ctgtacagca tgatactcaa cagccagacc
     1921 cgcttctata ttctgaaatc tgccagctat ggtaacactc tgtgggggaa cagcctgaat
     1981 gatccggctc agtgggagtt tgttggcatg aacaaaaaca aagcagttca gacagtaaaa
     2041 gataggatcc tggccgggcg ggcaaaacaa cccgttatct ttcatggtca gctgaccggg
     2101 aatatggatg tcgccattcc acaggtgccg gggggaagaa aggtaatctt tgatggtagc
     2161 gtgaacctgc cggaaggtac cctgagtcag gacagtggca ccctgatatt ccagggacat
     2221 ccggttatcc atgcctccat cagtggcagt gcaccggtca gcctgaacca gaaagactgg
     2281 gaaaaccgtc agtttacaat gaaaacactg tcgctgaaag acgctgactt ccatctttca
     2341 cgtaacgcct cgctgaacag tgacattaag tcggataaca gccatatcac actgggaagt
     2401 gacagggcat ttgtggataa aaatgacgga acaggaaatt atgtcattcc ggaggaaggt
     2461 acctctgtcc cggacaccgt gaatgacagg agccagtatg aagggaatat tacgctgaac
     2521 cataactcag ccctggatat cggcagcagg ttcaccgggg ggattgacgc ttatgacagt
     2581 gccgtcagca tcacctctcc ggacgtcctg ttgacagccc cgggtgcttt tgccggcagt
     2641 tcactgacag tgcatgatgg cggtcatctt acagcactga acggtctttt cagcgacggg
     2701 catattcagg ccggtaagaa cggcaaaatc accctgagcg gtacaccggt taaagatacg
     2761 gctaatcagt atgcccctgc tgtatatctg acggacggat atgacctgac cggcgataac
     2821 gcagcactgg aaattacccg tggagcacat gcttccggtg atattcatgc ctctgcggca
     2881 tcaacagtta ccatcgggtc tgacacgccg gcagaactgg cttctgcgga aacggctgca
     2941 tcggcgtttg ccggcagtct tcttgagggc tataacgcag cattcaatgg tgccataacc
     3001 ggtggcaggg ctgatgtcag tatgcataat gcactgtgga ctctgggtgg ggactctgcc
     3061 atccacagtc ttaccgtcag aaacagccgt attagttctg aaggagaccg tacattccgt
     3121 accctgacgg tgaataaact ggatgcaaca ggcagtgatt ttgttttgcg tacggacctg
     3181 aaaaatgccg ataaaattaa tgtgactgaa aaagccactg gttcagataa cagcctgaac
     3241 gtcagcttta tgaataatcc tgctcaggga caggccctga atattcctct ggtcacggca
     3301 ccggcgggaa cttcagcaga gatgtttaag gccggcaccc gggtgacagg tttcagtcgg
     3361 gtgaccccaa ccctgcatgt tgataccagt ggtggcaata cgaagtggat actggatggt
     3421 tttaaagcgg aggctgataa agccgctgcc gcgaaggctg acagtttcat gaatgccggg
     3481 tataaaaact tcatgacgga agttaacaat ctgaacaaac gtatgggtga cctgcgtgac
     3541 acaaacggtg atgccggtgc ctgggcgcgc atcatgagtg gtgccggttc tgcagacggt
     3601 ggttacagtg ataattacac ccatgttcag gtcggctttg acaaaaaaca tgaactggac
     3661 ggtgtggacc tgtttaccgg tgtcacgatg acctataccg acagcagtgc agacagccat
     3721 gcattcagcg gaaagacgaa atcggtgggg ggcggtctgt atgcttcagc attgtttgag
     3781 tccggtgcct atatcgattt gattggtaaa tatattcacc atgacaatga ttacacaggt
     3841 aactttgcta gcctgggaac gaaacactac aacacccatt cctggtatgc cggtgctgaa
     3901 acgggttacc gctatcacct gacagaggac acgttcattg agccgcaggc tgaactggtt
     3961 tacggcgccg tgtccgggaa aacattccgc tggaaagacg gtgatatgga cctgagcatg
     4021 aagaacaggg acttcagtcc gctggttgga agaacagggg ttgaactggg caagaccttc
     4081 agtggtaagg actggagtgt gacggcccgt gccggaacca gctggcagtt tgacctgctg
     4141 aataatggag ataccgtact gcgtgatgcg tccggggaga aacggataaa aggagagaag
     4201 gacagccgga tgctgtttaa tgttggtatg aatgcgcaga taaaggacaa tatgcgcttt
     4261 ggtctggagt ttgagaagtc agcctttggt aaatataacg tggataatgc ggtaaacgcg
     4321 aatttccggt atatgttctg atgtgggggt aaagggctgc cggaaggcag cctttctcag
     4381 agactcttaa aatataaaac tgaaggtctt cggtaagggg gagtgtatta gacgtcaact
     4441 agttttgccg actggcgcga gcctgtttaa gattctgtgt aaatgccttt ctcagaagtg
     4501 accgtccagg cggtcaccga actcgataat aaagcggctc attgccattc gccagtcctt
     4561 caacggcatc gtccatttct gggacgcaga ctggattgcc agccacacca cttttttcac
     4621 cgagtcgtct gtcgggaaca ctttacgctt tttgatcgca tggcggatca cgctgtttag
     4681 cgactcgatg gcattcgtcg tatagatcac tttgcggatg tccgttggat aagc
//
LOCUS       DAJTUI010000188         4680 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTUI010000188 DAJTUI010000000
VERSION     DAJTUI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.189"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..517
                     /locus_tag="OO464_003975"
     CDS             <1..517
                     /locus_tag="OO464_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR6228916.1"
                     /translation="RAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITLLNQ
                     AVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFIN
                     QLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAV
                     LIEAMESRSLR"
     gene            714..2153
                     /gene="aldA"
                     /locus_tag="OO464_003976"
     CDS             714..2153
                     /gene="aldA"
                     /locus_tag="OO464_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR6228917.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2195..3195)
                     /gene="gap"
                     /locus_tag="OO464_003977"
                     /pseudo
     CDS             complement(2195..3195)
                     /gene="gap"
                     /locus_tag="OO464_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3384..3914
                     /gene="cybB"
                     /locus_tag="OO464_003978"
     CDS             3384..3914
                     /gene="cybB"
                     /locus_tag="OO464_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR6228918.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4159..4332
                     /locus_tag="OO464_003979"
     CDS             4159..4332
                     /locus_tag="OO464_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228919.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4404..4553)
                     /gene="hokB"
                     /locus_tag="OO464_003980"
     CDS             complement(4404..4553)
                     /gene="hokB"
                     /locus_tag="OO464_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR6228920.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
BASE COUNT         1197 a         1076 c         1179 g         1228 t
ORIGIN      
        1 cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata ttccgcatga
       61 aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct ttagcatcac
      121 gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg accccaccat
      181 gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg atgcaccacg
      241 ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca gtgtaatctt
      301 tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac tcactggcga
      361 gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag attttatcgt
      421 tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac atgatgcggt
      481 gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt gtgaaccact
      541 tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct tttacacttg
      601 tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg atgattgatg
      661 ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg cccatgtcag
      721 tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt ggagacgcat
      781 ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc gatggtcagg
      841 ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa tgggaagcgt
      901 tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc cgcgaacgcg
      961 ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag ctagctgaag
     1021 tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca cggcgttacg
     1081 agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt aaacgtgcgc
     1141 ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt gcccgcaaaa
     1201 tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa tttacgccaa
     1261 acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc ggcgtgttta
     1321 accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac ccaaaggtcg
     1381 caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg actgcggcga
     1441 aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc gtaatggacg
     1501 atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt aatagtgggc
     1561 aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat cagttcgtca
     1621 atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa cgcaacgaca
     1681 ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa aaagtggcgc
     1741 gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag gggaaaggat
     1801 attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt atgcatgagg
     1861 aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat gctatctcaa
     1921 tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat ctgaacgtcg
     1981 cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt gaaaacttcg
     2041 aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc gcagatggta
     2101 aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct taatgagtga
     2161 aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat ttttcgaggg
     2221 tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc gttttcacca
     2281 gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact gaaccgaaat
     2341 ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat gactcgttat
     2401 tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt ttccgagaat
     2461 ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat gacctttcag
     2521 tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg tagtgtgggg
     2581 aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg ggccatccac
     2641 cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt ctatcccgaa
     2701 actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg acgccacgga
     2761 aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga tggttttcat
     2821 ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat gcgcctgcga
     2881 tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc ctttcgcttt
     2941 ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc catcaacgat
     3001 aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg aatcatgttt
     3061 cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa cgtctatgtt
     3121 gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa aaccgttaat
     3181 accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt gatcctttcc
     3241 aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa cgtaacgtac
     3301 tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg ttaacggaaa
     3361 attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa atcagcattc
     3421 actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt cgtggtttct
     3481 tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc atctcaattc
     3541 tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg ccgattatac
     3601 ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg atttatctcc
     3661 tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc aacccgtggt
     3721 ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg gttgatagct
     3781 taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg ttgcacgctg
     3841 cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc atgatgccgc
     3901 gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac ccgtggtaca
     3961 ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat gacaaataaa
     4021 accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac ccgatctttg
     4081 atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgcac ataagattcg
     4141 ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg aacgcttgtt
     4201 tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg gatctcacac
     4261 tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc gaaaaaaaca
     4321 tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag tcagatgcgc
     4381 ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc agcaacctcc
     4441 ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag tgtgaatgcc
     4501 agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgctt catagccttt
     4561 ctccttgccg gatggcgggt aagaggctaa caatctgaat tgcttggttc attcgttggc
     4621 ctcggtttgt actgacccca aaaagttgga cagttaaaca cgaggcatat aggtctgatt
//
LOCUS       DAJTUI010000189         4675 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTUI010000189 DAJTUI010000000
VERSION     DAJTUI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.190"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(337..582)
                     /locus_tag="OO464_003981"
     CDS             complement(337..582)
                     /locus_tag="OO464_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228921.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1359..2201)
                     /locus_tag="OO464_003982"
     CDS             complement(1359..2201)
                     /locus_tag="OO464_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR6228922.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2286..2483)
                     /locus_tag="OO464_003983"
     CDS             complement(2286..2483)
                     /locus_tag="OO464_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228923.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2503..2991)
                     /locus_tag="OO464_003984"
     CDS             complement(2503..2991)
                     /locus_tag="OO464_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228924.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(2988..3365)
                     /locus_tag="OO464_003985"
     CDS             complement(2988..3365)
                     /locus_tag="OO464_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR6228925.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            complement(3412..3789)
                     /gene="cbtA"
                     /locus_tag="OO464_003986"
     CDS             complement(3412..3789)
                     /gene="cbtA"
                     /locus_tag="OO464_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCR6228926.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            complement(3869..4090)
                     /locus_tag="OO464_003987"
     CDS             complement(3869..4090)
                     /locus_tag="OO464_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR6228927.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4177..4653)
                     /locus_tag="OO464_003988"
     CDS             complement(4177..4653)
                     /locus_tag="OO464_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR6228928.1"
                     /translation="MQQLSFLPGEMTTRERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
BASE COUNT         1093 a         1120 c         1230 g         1232 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgttgg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctgggtggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gccggactcg gaatcgaaaa tttcttttat
     1261 catattgata ttaatgcaat ttgtttggtt tgattaaaaa tgtaccctca attatacccg
     1321 cagtcccttt tgatatcgaa agggaatcac tctcgggatc aggctgagag cacagaagga
     1381 aagtacctgg ctattatatc gttaattctg tcaatcagtt ccagcctttt gaaagtgatg
     1441 tgtgcagatc ccttcttaaa gtatttaatg atgaacatct cgtcttcata gcgctcttta
     1501 ccctgaacgg actggatgtg accatccaga cgaatagtga tgttttcccg gttgtcgggg
     1561 atcggtttgc cgctgaacag atgtagcatt ctttccaggt cgacgagtcg gtcagtctgt
     1621 tgcccggtga taagatgaaa cccccaccgg tcccacctca ccaggttgtt gacgataatt
     1681 ttactgccaa atttacaggg tgagttggtt ttgtaatccc agctcagccc tttgaagacg
     1741 ttgatcactc cccgttcaaa cacttcatct ttgttctggt gcagttgttc aaatgtgctc
     1801 agaatgttcg cttcgctgat ttccggaaaa ttatcatatt ccagtgacct gtaccatgtg
     1861 tcccgggtct gcgcatccat cagactcagc atcccggaac gttgcatcag atcacgccag
     1921 agttcgcgat caagattgcg tgtgatggct ttcattgcgg tttccggttt ctccatcagc
     1981 cagcaaccgc agcgaaaatc ctgtttcatg gcccagtcca gagccgtttt acctctaata
     2041 ctttctgtca ttgctgatac ttcagccagt tgtcggataa gtgtgtcgat ttgcagcaat
     2101 gtactctttc gtacagccag aatgtggcgg atactggttg agcagataac gtccgtatgc
     2161 ccgttgacca cttccggacg agaggctgtg atttcagaca taatcgatat tcctttttgc
     2221 agataaacga aaacgccagc cgtattcagg ctggcgtcag tggaatgaag tctggtgagt
     2281 aagactcacc ggttctgttg cagaaactgc agctgacgca gggtacggat atcttccctg
     2341 gcctgttcaa tacagggcag cagtgctgcc gaatccgttt tatcctcatc gttgtcgaag
     2401 ataatccctg attcgcagtc aatattgtcc tgcagccacg caatcagaat atccagcgcc
     2461 gtttctgtgg ttaataattt catccagtgt cttttgtcct gattaccagt cgaaagtggg
     2521 gaaccccggc agacattctg cgccggcgtc cagatgaatg agactgacgc cataacgccg
     2581 ggtgagagtg acgaccagac gacggaacgt tctggacagg cccaggcgtt taagccgcag
     2641 aaccgggtac gaccatgcat cggtacgtaa cagatagccg ctgcctgtaa aatgaatcca
     2701 ttctgtttcg ccgtcgtcat tagtctggtg tgacagcgag tacagccagg cgttgtccgt
     2761 ttccgtgata tgtgcggtgc tgcaacgtat gcaggtaagg gttgttaacg gtggtggtgt
     2821 actggttgcg tgttggtcgg atttatcgac cacgcggagt gaataaccgt tttcagagac
     2881 cttgttaatc agttcactga gattaacacc atcgacgtca acgacaatgc gccccatgtt
     2941 cagtgcctgc acgttaacgc tgtcggcttc cagcgtcagg gccagtttca ttgtttcgct
     3001 tccggatgct tcccccgggt aatgttattt accgttctgt agttatcgcg ggtcatcagg
     3061 ccggttgccc ggcgtgcccg gagaatatca atgctgttga ttaactgcga cggggctcct
     3121 gcgctgaatc ccggctggtc ggtacgtacc agcgcgtatt tttcaacgag aaagttcacc
     3181 gcgtcacaca gtgaaattcc tgcctcaata tgctgctcaa tcacacgttc atcagcgaac
     3241 ggtgtgtcat tcagcgtgag gccatagtgc tggtccagca gtcgggtgag cagtgtctgc
     3301 cagatggtga tgggagacgg gcagcccgat gcctcccgta cgtgagtgtc gggtaatgta
     3361 ttcatcttaa gttctctgta acaggtggtt ataaagggga gatatccggt gttatacggt
     3421 ggttgcgggc gctgccggtg tcggataaac agccagataa acgtaaccac aggagccgag
     3481 ggtgtcggct tcgcaggtca gccctttcgc atacagtgtg acggtatgct gatggcgggg
     3541 attcagttca ccgccagtga gcatgagttc cagttgtttc atcagcagcg gaaaggcctg
     3601 gtccaggtgg tacgcatcta cgtcgctgaa cctgcctctg ataccggcgc ggtctgcaag
     3661 gtaatgcagg cggttaccct cctgtaccag tcgggccccg aaacagggcg tcacggtgca
     3721 gggcagcccc caccaggggc ggtcgtgatt gtcgtcggga ggcgttgtcc cggggagtgt
     3781 gtctgacacg ataaagtcct cacagaaaat cggttaagaa tggtccggta gtggcgataa
     3841 ttctgctcat cagaattccc ttccagactc agttcagggt gacgctcatc agccggacat
     3901 acggaccaaa actgtcctta cggcgttctg caaacacagc cagtacaccg ggaatatcct
     3961 gcacttcacg gccggtatat gtatccgtgc taccgtgcca gcggtattta ccggtacaga
     4021 acggaaaaag acgggatgca ggatgctgac ggtgaatacg catggcttca ccacgggtga
     4081 tgattttcat gagaatagtc ctcctgaatg tgaaagtaag aaacagctga gaaagagaaa
     4141 ctgaagtgat atgaaggtga ttgtgacggt gacgggttaa agcagaccgt gttccgcaaa
     4201 ggagaaaacc tggctgccac cgactatcag atggtccggt acccggatat caaccagggc
     4261 cagtgcctgt accagacgct cagtgataag gcggtctgcc ttgctggggg ggacttcacc
     4321 ggacgggtga ttgtgcgcca gcaccacggc agcggcattg tggtacaggg cgcgtttaat
     4381 cacttcccgg ggatggactt ccgtacggtt gatggtgccg gtgaagaggg tttcaccggc
     4441 aatcagctga ttctggttgt tcagatacag cacccggaac tcttcacgct ccagtcccgc
     4501 catgttcaga atcagccatt cccgtgccgc atgcgtggag gtgaaggcca cgccgggttc
     4561 atgaagatgg cggtccaggg ttttcagggc ccgcagaatg aggctgcgct ctctggttgt
     4621 catttctccg ggcagaaagg aaagctgctg cattgtccct gccaccattc agtcg
//
LOCUS       DAJTUI010000190         4663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTUI010000190 DAJTUI010000000
VERSION     DAJTUI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.191"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..212
                     /locus_tag="OO464_003989"
                     /pseudo
     CDS             1..212
                     /locus_tag="OO464_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            355..576
                     /locus_tag="OO464_003990"
                     /pseudo
     CDS             355..576
                     /locus_tag="OO464_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(597..659)
                     /locus_tag="OO464_003991"
                     /pseudo
     CDS             complement(597..659)
                     /locus_tag="OO464_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1109..2521
                     /gene="ospC1"
                     /locus_tag="OO464_003992"
     CDS             1109..2521
                     /gene="ospC1"
                     /locus_tag="OO464_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR6228929.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2625..2774)
                     /locus_tag="OO464_003993"
                     /pseudo
     CDS             complement(2625..2774)
                     /locus_tag="OO464_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2850..4547
                     /gene="ospD3"
                     /locus_tag="OO464_003994"
     CDS             2850..4547
                     /gene="ospD3"
                     /locus_tag="OO464_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR6228930.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1625 a          838 c          865 g         1335 t
ORIGIN      
        1 ccgcagccgt caggctgcct actcgagtca caaggctctt tgtgtccgat atgccggcag
       61 cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt tatgggggcg
      121 gtgaagaatt atctaacctt gtcttgttac atccaaattg ccatcgacag ttacacagtt
      181 gtgaagccgg ctctctcaaa aagggcttat aaaggcttga gcggtgtacc gggaaaccgg
      241 tatgcaccgt tcttagggga gggcagtcca gcaatgaact gtccttaccc gacaaacatc
      301 caaaacgaac ggaacgtgct ggaatccgca tataacccgt tgtaaagtga ggaaatggac
      361 acctcacttg ctcatgagaa cgcccgcctg cgggcactgt tgcagacgca acaggacact
      421 atccaccaga tggctgaata caaccgcctg ctctcacagc ggatggcggc ttatgcttcc
      481 gaaatcaacc gactgaaggc gctggttgcg aaactgcagc gtatgcagtt cggtaaaagc
      541 tcagaaaaac ttcgcgcaaa aaccgaacgg cagattccat tttcacgggc gatctacgcc
      601 acacaggctc tgggttgcag tgactgctcc attaaggcga ttttgaactc ataagggaag
      661 taacaaaaaa actgcaaaag tacaataatt atatatggcc aaactaacct ctattaaaaa
      721 acatcagttt ttcacactta gttgagcttc atgaaaaaat catcgaggat ttcattgcag
      781 aatacacatt ccactaactg gaagcactcc ataagaggaa tcagagaaag tatcgttttt
      841 ttatagtaaa attcattgct gttcaataaa attgatatag ttattaaatt atgaaaataa
      901 tttcacaaaa aagattaact tgagttctta agcatcggat tttagatatt ttgaaatatc
      961 tggcatcaca taacatcaaa agtatataat actcttatct aattacatgc agtatattgt
     1021 atatatactc aaaaattgta attcataccc tgctatatct tgtagggaaa gatattaatt
     1081 aaaactgttt tcatataagg ttcattttat gaatatatca gaaacactga actcagcaaa
     1141 tacccaatgc aatatagatt ctatggataa cagattacat acattgtttc caaaagtgac
     1201 atcagtgcga aacgctgcac aacaaactat gccagatgaa aaaaatttaa aagatagtgc
     1261 aaatattatt aaaagtttct ttaggaaaac tatagcagca cagagttata gtagaatgtt
     1321 ctctcaaggc tctaacttta aatctttaaa tatagcaatt gatgcaccat cagacgctaa
     1381 agcctcattt aaggctattg agcaccttga cagattatcg aagcattata tatctgaaat
     1441 aagggaaaaa cttcatcctc tttctgcaga ggaactcaat ttgctttcgc taattattaa
     1501 ttctgattta atcttcagac atcaaagtaa ttctgatttg tctgataaaa ttttaaacat
     1561 taagtcattc aataaaattc agtctgaagg aatatgcaca aaacgaaaca catacgctga
     1621 tgatataaaa aaaatagcta atcatgactt tgtgtttttt ggcgttgaaa tctctaacca
     1681 tcagaaaaaa caccccctga atacaaaaca tcacactgtt gattttggtg caaatgcgta
     1741 tatcattgat catgactctc catatggata tatgacatta accgatcact ttgataatgc
     1801 tattccacct gttttttacc atgagcacca atcattttta gataaatttt cagaggttaa
     1861 taaagaagtt agtcgatacg tacatggaag taaaggaatt atagatgtac caatattcaa
     1921 tactaaagat atgaagttag ggctcggatt atacctgatt gactttatta gaaaaagtga
     1981 agaccaaagc ttcaaggagt tttgctatgg aaaaaatctt gcccctgtgg atctggatag
     2041 aatcataaac tttgtttttc agccagagta ccatatacct aggatggtaa gtacagaaaa
     2101 cttcaaaaaa gttaagatta gagaaatatc cttagaggag gctgttacag catctaatta
     2161 cgaagaaatt aacaagcagg tcactaacaa aaaaattgct ctccaggctc tttttctttc
     2221 gattactaat caaaaagagg atgtcgcctt atatatatta tctaattttg agataactag
     2281 acaagatgtt atttccataa agcatgagtt gtatgatatt gagtatctac ttagcgctca
     2341 taattcaagc tgtaaagtac ttgagtattt tatcaataag ggattggttg atgtaaacac
     2401 aaagttcaaa aaaactaata gtggggattg tatgttggat aacgcaataa aatatgagaa
     2461 tgcagaaatg ataaaactat tattgaaata tggtgcaaca tctgacaata aatatattta
     2521 atcaaaattg aatatcgttt agcaaaaggc agtatttgca caaaatatcg atatattgtt
     2581 tattgtcagt atggctcaat gtgataatag ttggaaagtt tgatgggttt cgccccgttg
     2641 tagcggtagt cgaactggaa ggtcttcagg cactgcttac agcgatagag cagcccccct
     2701 gaactggaat ggccgttccg gatacccccc tgagtttcag agtaacggtg acaaaccaca
     2761 tcaatctttg ccatcaatca tccaaagggc aaagagtaca acaacactaa gtctgcgtca
     2821 caacccatca atgaaaggaa tatatacata tgccatcagt aaatttaatc ccatcaagga
     2881 aaatatgttt gcaaaatatg ataaataaag acaacgtctc tgttgagaca atccagtctc
     2941 tattgcactc aaaacaattg ccatattttt ctgacaagag gagtttttta ttaaatctaa
     3001 attgccaagt taccgatcac tctggaagac ttattgtctg tcgacattta gcttcctact
     3061 ggatagcaca gtttaacaaa agtagtggtc acgtggatta tcatcacttt gcttttccgg
     3121 atgaaattaa aaattatgtt tcagtgagtg aagaagaaaa ggctattaat gtgcctgcta
     3181 ttatttattt tgttgaaaac ggttcatggg gagatattat tttttatatt ttcaatgaaa
     3241 tgatttttca ttccgaaaaa agcagagcac tagaaataag tacatcaaat cacaatatgg
     3301 cattaggctt gaagattaaa gaaactaaaa atggggggga ttttgtcatt cagctttatg
     3361 atcccaacca tacagcaact catttacgag cagagtttaa caaatttaac ttagctaaaa
     3421 taaaaaaact gactgtagat aattttcttg atgaaaaaca tcagaaatgt tatggtctta
     3481 tatccgacgg tatgtctata tttgtggaca gacatactcc aacaagcatg tcctccataa
     3541 tcagatggcc tgataattta cttcacccca aagttattta tcacgcgatg cgtatgggat
     3601 tgactgagct aatccaaaaa gtaacaagag tcgtacaact atctgacctt tcagacaata
     3661 cgttagaatt acttttggca gccaaaaatg acgatggttt gtcaggattg cttttagctt
     3721 tacaaaatgg gcattcagat acaatcttag catacggaga actcttggaa acttctggac
     3781 ttaaccttga taaaacggta gaactactaa ctgcggaagg aatgggagga cgaatatcgg
     3841 gtttatccca agcacttcaa aatgggcatg cagaaactat caaaacatac ggaaggcttc
     3901 tcaagaagag agcaataaat atcgaataca ataagctgaa aaatttgctg accgcttatt
     3961 attatgatga agtacacaga cagatacccg gactaatgtt tgctcttcaa aatggacatg
     4021 cagatgctat acgcgcatac ggtgagctca ttcttagccc ccctctcctc aactcagagg
     4081 atattgtaaa tttgctggcc tcaaggagat atgacaatgt tcccggactt ctgttagcat
     4141 tgaataatgg acaggctgat gcaatcttag cttatggtga tatcttgaat gaggcaaaac
     4201 ttaacttgga taaaaaagca gagctgttag aagcgaaaga ttctaatggt ttatctggat
     4261 tgtttgtagc cttgcataat ggatgtgtag aaacaattat tgcttatggg aaaatacttc
     4321 acactgcaga ccttactcca catcaggcat caaaattact ggcagcagaa ggcccaaatg
     4381 gggtatctgg attaattata gcttttcaaa ataggaattt tgaggcaata aaaacttata
     4441 tggaaataat aaaaaatgaa aatattacac ctgaagaaat agcagaacac ttggacaaaa
     4501 aaaatggaag tgattttcta gaaattatga agaatataaa aagctgaata ttatctcgtg
     4561 gtgatacact gaatttgagg taaccggctc atttaaaccg tctggtctgt tacctccggt
     4621 tccacaaaaa taatgtccat cattttgaag tggacactat cgt
//
LOCUS       DAJTUI010000191         4592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTUI010000191 DAJTUI010000000
VERSION     DAJTUI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.192"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(68..856)
                     /gene="phoH"
                     /locus_tag="OO464_003995"
     CDS             complement(68..856)
                     /gene="phoH"
                     /locus_tag="OO464_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR6228931.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1463..2728)
                     /gene="efeB"
                     /locus_tag="OO464_003996"
     CDS             complement(1463..2728)
                     /gene="efeB"
                     /locus_tag="OO464_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR6228932.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2734..3861)
                     /gene="efeO"
                     /locus_tag="OO464_003997"
     CDS             complement(2734..3861)
                     /gene="efeO"
                     /locus_tag="OO464_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR6228933.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3919..>4592)
                     /locus_tag="OO464_003998"
     CDS             complement(3919..>4592)
                     /locus_tag="OO464_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR6228934.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1075 a         1295 c         1108 g         1114 t
ORIGIN      
        1 gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct ctgcaccgat
       61 aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt acgcaatcct
      121 cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc agcgcgtcac
      181 ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg ttgacgataa
      241 ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca gtcacattct
      301 gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata taggcgaacg
      361 gcgcggtttc caccttccct atttccggtc gcaggcagta ctgcataaag gaagccccta
      421 agcgccggac cagcaagtcg tagaccgggc gaaaataggg agcaaacttt tctgcgatat
      481 caccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg acgataatcc
      541 tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc caggtttttc
      601 cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc tgcagatagt
      661 gcaattgcgc ttcatttcgc gcaaggatgg gcgaggtatc gcgactgtcg cgggccatac
      721 caatggcttc tacgccgctc atctgcacaa gcgaggtgac cgattcttct tcacgctgct
      781 tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct ttgatcactg
      841 ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac gcgccgttgg
      901 cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca tagtttgctt
      961 ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta acacgagtaa
     1021 agaaaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga aatctccgcg
     1081 agtggtggag cgttgcttaa caaaacattg tccagaacag gacgttacca tccgcgatct
     1141 ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag tacaacagaa
     1201 ctcgcattca ttgcaacaat atattcactt atatttaacg ataacaccca tttcggtgac
     1261 attaatttct actggaaaga tgacagagtg atgacagtga tggaaaaagc tgtgtgcttt
     1321 ccgcaggatt cgcagggctg gtaaaatgtg cctcgtgttt gccggattcg gcgcgaacgc
     1381 cttacccgac ctacaagtga ttaataattt gtgcacaaaa ctcatgcccg ataaacctgc
     1441 gcatcgggca tatttgcgtc gttcaaacct gcaatagcgc ccttcccaga taatcgttcg
     1501 cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta acgtattcct
     1561 ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc agatcgtgtt
     1621 ggtagcagac aaacagcaac cccatatcca gttgtccgga gttggtgacc cccaacgaat
     1681 aactgtagcc gcgacgcagc atcaggctgg actcactctc cgccgtgcgg ggattcgcca
     1741 gccggatatg gctgtccagc gcgatcacct tcccttccgg gtcgctggcg taatcaggca
     1801 catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacgg ccaaaaatcg
     1861 tctgctgttc tttcagcggc gttctgtccc aaaattccac tcgaaactga atcaagcgta
     1921 ctgcctgata gctgccaccg attgtccacg caggctcctg ctgatctgcc gttacccaca
     1981 ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg tctttgaaac
     2041 ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg gaaataaacc
     2101 cttcccgctt ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata tcgcgcagcg
     2161 catggttcac cgtgtcctgg gtgttggcgc aaatctgtag caacacatca ccatgacata
     2221 acgccgcatc cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt tttggcatct
     2281 gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac gtgatggtga
     2341 gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc aggcgcggat
     2401 ttggcgtttc tggtgctgct ccgccctgac tcagaaaagc aaaacgctga gtcaacaagc
     2461 gaaacaaccg ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc accagcatca
     2521 ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac ggctgtttct
     2581 cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt gcatgagcga
     2641 ccggacaact tcccgccagc gcaccaatcc ctttcagtaa acgtcggcgt gacggttcat
     2701 tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca caccgcgaag
     2761 ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc ggtcggcatc
     2821 ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca gaatggtatc
     2881 gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt tttgcagttg
     2941 tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga aatcccacag
     3001 atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca cttcctcaat
     3061 cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac tgatgcgttt
     3121 ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc ctttggtggt
     3181 gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg ggtcggcggc
     3241 tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg agaacagttc
     3301 agcaatcggt tcaatacgct catagtgctg gcgcgtcggc gcatacagtg ctttcgcttt
     3361 ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca ccagctgcgt
     3421 ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac ttaacagcgc
     3481 atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc ctttcgggtt
     3541 agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg tcattttctg
     3601 gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt tgaggatctc
     3661 ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc cggcgttaac
     3721 cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct gcggcacatc
     3781 agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact gcaatgcgtt
     3841 acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt aactaagcaa
     3901 gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc gtggtggcag
     3961 agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt cgctgacgct
     4021 cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg agtgagttga
     4081 gaggaccgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc cggcttcatg
     4141 aaatgcgcga atggcaccag ccgccagccc tgcggcgacg aagagaataa acaggctggt
     4201 ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca ggaagccgag
     4261 caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc cgacatcttg
     4321 ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa cggcaaaaaa
     4381 gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc tatcgactgc
     4441 ctgttccagt tgcactttga cgttgcgcga cactttgcgc atccagaaaa ccatccaggt
     4501 aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgct ctttttgcgg
     4561 aaattcgccg gtggtttcgt taatgaagat cc
//
LOCUS       DAJTUI010000192         4542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTUI010000192 DAJTUI010000000
VERSION     DAJTUI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.193"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            126..1214
                     /locus_tag="OO464_003999"
     CDS             126..1214
                     /locus_tag="OO464_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR6228935.1"
                     /translation="MSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLI
                     FVASLLCWCVGISNYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSF
                     EATFPQALEDVGVTNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRL
                     KMTNNNAHHQCEKWFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQ
                     VRIPKTVRLGILPVIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKY
                     LTQAERLERDQHLLIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRR
                     GDYLTAEKIRKRAARVFVNDERFTRGGN"
     gene            1214..1948
                     /locus_tag="OO464_004000"
     CDS             1214..1948
                     /locus_tag="OO464_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6228936.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2415..3176
                     /locus_tag="OO464_004001"
     CDS             2415..3176
                     /locus_tag="OO464_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6228937.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3220..4299
                     /locus_tag="OO464_004002"
     CDS             3220..4299
                     /locus_tag="OO464_004002"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6228938.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4296..>4542
                     /locus_tag="OO464_004003"
     CDS             4296..>4542
                     /locus_tag="OO464_004003"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6228939.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDWICPY"
BASE COUNT         1246 a          864 c         1119 g         1313 t
ORIGIN      
        1 gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc ggcgtagcgt
       61 tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg ttgtcgttat
      121 accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt ggtttatttt
      181 gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt ccccttagcg
      241 gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt atctcaaact
      301 atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat gaccagcgta
      361 tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc ggttgggggt
      421 taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg acaaacatag
      481 agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct gaaggtggca
      541 tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg tgtatatgcc
      601 gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt attgccttaa
      661 tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg tctgttcccc
      721 atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg atgaatcagg
      781 tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc attatgaccg
      841 ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc agagctgaag
      901 ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa tacctgacgc
      961 aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg gggttcgttc
     1021 gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg gcaatccaca
     1081 atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt gattatctga
     1141 cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa cgttttactc
     1201 gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca caaacatcaa
     1261 ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga ttggcctgga
     1321 tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag tgaaacttca
     1381 ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca tcgtgatatg
     1441 ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga aggtttgtcg
     1501 tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag gaatgacgaa
     1561 tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag tggatatttt
     1621 attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt tgtttataaa
     1681 acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga ttagcacacg
     1741 taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg agtccgcaat
     1801 tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac gtaatgctca
     1861 acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggtatg aaagatgtaa
     1921 caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct ctttttcgat
     1981 gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat gttttgccac
     2041 gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa agagagttaa
     2101 taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt tgttttttaa
     2161 attttcaggt tttggttaaa tatttgcttg caaatatagg tgatttttta ttgtaggctt
     2221 ttgaaggcta ttataactgt gcgctaaatt aatgcgttga ataaaagaaa taacaaaatt
     2281 aagaaaagcc atatttacga tatgggcaac ataaatataa tctaaaaaag agtaaggcgt
     2341 tgtaggttgg ttccgcgttc attaccttgt tagtgaacgc ggatttatca gttgtcgaaa
     2401 tggtttttat aataatgaaa aattttaatg tactaaccaa agtcgtattt tgcttgtcgt
     2461 ttttatcaag cgcatttccc acctatgctg cggatgatgt caaagtgact caaaacactt
     2521 cagtttttaa actgaagctt agtgcaacgc gattaatcta tgaacaaggg tcggatggcg
     2581 cagcgataac tgtgacaaat ccgcaggatt ggccgatgct tgtgcagagt aaagtctttg
     2641 aagaagataa agtaactgca gcgcccttta ttgtcactcc gccactgttc aggcttaatc
     2701 caggccagca aaataaaatg tgggttgtgc agactgcgcc agctttacaa gacagggaaa
     2761 aattacactg gctatgcgcc accggcatta cgcctaaaaa gagtgatgtt tgggccgaga
     2821 aagataaaga aaaaattgct gaaaatgtga acctgaacat ccaggttaac gtacatacct
     2881 gcataaaact tattaccagg ccatctggta ttgaaggcaa gatggttaat gcgagtgaga
     2941 agttatcgtg gaaaaagcag ggtgataaaa ttgtcgcggt caatcagtcg ccgttctaca
     3001 tcaacctttc ttctttacag gttggcggta gcaagattga gaaactagat tatattgcgc
     3061 cgttttcgca acatgagttt gttatgccaa aaactagcgg ccaggatatt aaatggtctg
     3121 tgattactga tgaaggtggt gagagtcgtt tgtttatatc tcatttgcaa tcataatgta
     3181 ttacattttt attgaatatt tttatgccat gcatattcca tgttttatcg actgtcatta
     3241 attttgataa tggccttact tgcgggtcag ttaagtgccc aggagtggtc atttgattct
     3301 tcacagttgg aaggaaacgt tagtgcggac actgtggcaa tgtttaatca gggagagcaa
     3361 ctccccggaa attatcgtgt ggaaatttat cttaacggtg aaaaagttga tgttggggag
     3421 tttccctttc atcgtccgga atcccctgaa gaaaaagagc ttgttccatg tttaaccgtt
     3481 gatgacctta tccactatgg cattaaaatt gataaaagca gcagtgatac tgacaataaa
     3541 aagaatcagt gttttaaatg gaactctatt gaaggtttga aagtaaatta tgattttgat
     3601 agccagcgcg tacagataac cgttccacaa ctgtatctgc aagataaaaa aagtagttta
     3661 gcaccagtgt cgttatggaa tgagggtgta gctgcattcc gcatggtgta tcagacaaat
     3721 attgatattt ccaaacaaaa tgataatcaa tctaccacgc gtaattctcg ttatggcagg
     3781 ttcactccag ggtttaatct tggtgcctgg aggtttcgta gttcagtgac atggagcaag
     3841 gaacttggcc agtcagaaag atggcaaagg ggttacatgt ggtttgagcg aggcataaat
     3901 gcgattaaaa gccgattaac gcttggagaa tcttatacgt cgtctgaggt gtttgacagc
     3961 ataccttttc gcggcgggat gttagcgacc gacgatgcga tgactccgcc ggaggacagc
     4021 tactacacac ctgtggtgca tggaatagca caaagtgagg cgcaggttat tattaaacaa
     4081 aatgggcaaa ttatttttac ccgttctgtg ccgccagggc cttttgcgct ggataattta
     4141 ccaacgctgg ccgttggcgg ggagcttgat gttacagtga gagagagtaa tggcgaggag
     4201 cagtattttt ccgtgccatt tcaaacgccg gccatcgctc tgcatgaagg atatttcaaa
     4261 tatagtgtga tgggggggaa tataaagaag aaggtataaa aagctcccct gtctccgtgg
     4321 gcgaaatcac ggcgatgtac gggcttcctt atggcgtcac ggtgtatgga gggattcaaa
     4381 acgccactca ttttaatgca atatctacgg gtattggcat ttctctgggg ctgttaggga
     4441 gtttatcgac agatattacg cgttcgattg cgaatttata ttatgggaat aaatatcgta
     4501 ttcgttatag taaatccatt agtgattgga tttgccccta ta
//
LOCUS       DAJTUI010000193         4455 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTUI010000193 DAJTUI010000000
VERSION     DAJTUI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.194"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..2388
                     /locus_tag="OO464_004004"
     CDS             <1..2388
                     /locus_tag="OO464_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR6228940.1"
                     /translation="IVLNQTIVDTRNVNFVEITPEKGIAACLTTESLDAMGVNTDAFP
                     AFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNARGYVPPERWDEGIN
                     ALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNSTWSRSSGQTAEWK
                     NLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNMLPDSLKGFAPVVR
                     GIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVEIKEADGSVNSYSV
                     PFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGPWGTTWYGGGQYAE
                     YYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLYAKTLNQLGTNFQL
                     MGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFYTKRGKLQVNISQQ
                     LGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNYSKSRGQPDADQVF
                     ALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLGLTETLLDDGNLSY
                     SVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNYALSGSLVAHSQGI
                     TLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSYRENRIALDAASLK
                     RNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRFGAIATLDGIQTNS
                     GIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINSAITRMDAICR"
     gene            2379..3362
                     /locus_tag="OO464_004005"
     CDS             2379..3362
                     /locus_tag="OO464_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6228941.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3460..4000
                     /locus_tag="OO464_004006"
                     /pseudo
     CDS             3460..4000
                     /locus_tag="OO464_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4008..>4455
                     /locus_tag="OO464_004007"
     CDS             4008..>4455
                     /locus_tag="OO464_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6228942.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLR"
BASE COUNT         1182 a          984 c         1155 g         1134 t
ORIGIN      
        1 attgttctga atcagacaat tgtagatacg cgcaacgtca attttgttga gataacgcca
       61 gagaagggga ttgccgcctg tttgacgact gaaagcctgg atgcaatggg tgttaatact
      121 gatgcgtttc cggcttttaa acaactggac aaacaagtgt gtgtgccatt ggcggagatt
      181 attccggatg ccagcgtaac gtttaatgtg aataaactcc gtctggaaat ttcagtaccg
      241 caaatcgcca tcaaaagtaa tgcccgtggt tatgtccccc ctgaacgttg ggatgaaggg
      301 atcaacgcgc tattactggg atattcgttt agcggggcta acagtattca tagcagcgca
      361 gacagtgatt ctggcgacag ctattttctg aatttaaaca gtggcgttaa tttaggccca
      421 tggagattac gcaacaattc aacatggagt cgcagtagtg gccaaaccgc agaatggaag
      481 aatctcagca gctatttgca gcgggcggtt attccactaa aaggcgaact gaccgtaggt
      541 gatgattata ctgcaggcga ttttttcgat agtgtcagct ttcgtggtgt gcagctggcg
      601 tcagatgaca acatgctgcc agacagcctg aaagggtttg cgcctgtggt gcgtggtatc
      661 gccaaaagca atgcccagat aacgattaag caaaatggtt acaccattta ccaaacttat
      721 gtatcgcctg gtgcttttga aattagtgat ctctattcca cgtcgtcgag cggtgatttg
      781 ttagttgaaa tcaaagaagc ggacggcagc gtcaatagct acagcgtacc gttttccagc
      841 gtgccattac tccagcgtca ggggcgaatc aaatacgcgg tgacactggc gaaatacaga
      901 accaatagta atgaacagca ggagagcaaa tttgcccaga ccacgttgca gtggggcgga
      961 ccgtggggaa cgacatggta tggtggtgga caatatgctg aatattaccg tgccgccatg
     1021 tttggtctgg gatttaacct tggcgatttc ggagcaattt cgttcgatgc gacccaggcg
     1081 aagagtacgc tggcagacca aagcgaacat aaaggtcagt catatcgttt tctgtatgcc
     1141 aaaacgctca accaattggg cactaatttt caattgatgg gctatcgcta ttcgacgtcg
     1201 ggtttctaca ccctttccga caccatgtat aaacatatgg atggctacga atttaatgac
     1261 ggtgatgatg aagatacgcc gatgtggtcg cgttattaca atttgtttta caccaaacgt
     1321 ggcaaactgc aggtcaatat ctcccagcaa ttaggcgagt acggttcgtt ttatttaagt
     1381 ggtagccagc aaacttactg gcataccgat caacaggatc ggctattaca gtttggctac
     1441 aacacgcaaa ttaaagatct ctcgctgggg gtttcctgga actacagtaa gtcccgtggt
     1501 caacctgatg ctgatcaggt gtttgcacta aatttttccc tgccgctcaa tctgttgctc
     1561 cccagaagta atgatagcta taccaggaaa aaaaattacg cctggatgac ctctaacacc
     1621 agtatcgata acgaagggca cattacacaa aacctgggtt taacggagac actactcgat
     1681 gacggtaacc tgagctacag cgtgcaacag ggatataaca gcgaggggaa aacggctaat
     1741 ggtagcgcca gcatggacta caaaggggcg tttgcagatg cccgagtggg ctacaactac
     1801 agcgataacg gcagtcaaca acaactgaac tacgctcttt caggcagttt agttgcccat
     1861 tcacagggca ttaccctggg gcaatcgctg ggggaaacta acgtgctgat tgcagcacca
     1921 ggcgcagaga atactcgtgt ggcgaacagc accgggctga aaactgactg gcgcggatat
     1981 accgttgtcc cctatgccac ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg
     2041 ttaaaacgta acgtggatct tgaaaatgca gtagtcaacg tggttcccac caaaggggcg
     2101 ttggttctgg cggagttcaa tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag
     2161 cagggtatac cgctgcgttt tggcgcgata gcgacgctgg acggcataca gactaatagc
     2221 ggcattattg atgatgatgg ctcgctctat atgtccggtt tgcccgctca gggagcgata
     2281 actgtacgct ggggcgaagc tcccgatcaa atttgtcata tcagttacca gcttaccgaa
     2341 caacaaatta actctgcgat tacgcgaatg gatgccatat gcagataatc tttggagaaa
     2401 aatgcgtgtt attactacga ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa
     2461 ctgctgctta tatcgggcag tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt
     2521 ttggcgttaa aactctggat gaagaagaaa atacggctgg agtagttaaa gacaaatttt
     2581 atcagtggaa cgaatcgaat gattattatg tttcctgtga ttgcgataaa gacaatgtca
     2641 gaagtggccg atgggcattc gccgcggatt caccgttagt ctatttaggc gacaactggt
     2701 acaaaattaa tgactatctt gccgccaaag ttttattgca ggttaaaggc agttctccta
     2761 ctgcggttcc tttcgaaaac gtgggcacag gggcagatac acgatggcat atttgcgatc
     2821 ccggcggtca acgtttaggt ggccaggggg ctagcggtaa tagcggtagc ttttccctga
     2881 aaatattgca gccgttcgtt ggttcggtcg tcattcctcc tatggcgctg gcgcgattat
     2941 ttgaatgcta caacataccc gcaggtgatt cctgcacgac tactggtaca ccggttttag
     3001 tgtattacct gtctggtacg atcaattcac ttggctcatg ttccgtcaat gccggagaaa
     3061 caatcgaggt tgatctgggc gacgtatttg cggctaactt tcgtgttgta gggcataagc
     3121 ctcttggggc cagaacggca gaacttgcaa ttccggtcag gtgtaacacg ggaaacgcag
     3181 ggttagttaa cgtcaatctg agtctgacgg caacctcaga ccccagctat ccccaggcga
     3241 ttaagacgtc acgtcctggc gtgggcgtgg tggtgaccga tagccagcac aacattattt
     3301 cccctgctgg tgggacacta ccgctctcta ttcctgatga tgcagacagt atccgcgaat
     3361 gaatgtctat ccagtcagca cgacaagcgt accaccagaa accgggcgat ttgaagccac
     3421 ggcaacggtg agaataaatt ttgattaacg cgtgaacgta tgaagaaaaa aacgatatat
     3481 cagtgcgtta ttttgttctt tagccttctt aacatccatg tcgggatggc tgggcctgaa
     3541 caagtcagta tgcatattta tgggaatgtg gtcgatcagg gctgtgatgt cgccaccaaa
     3601 agtgcattac aaaatattca tattggtgat tttaatatca gtgattttca ggccgcgaat
     3661 accgtaagca ctgctgctga tttgaatatt gatatcaccg gttgtgccgc tggtattact
     3721 ggcgcggacg tcctttttag cggcgaggct gacacccttg cgccgacact gctcaaacta
     3781 actgacacag gcggaagcgg tggtatggca acggggattg ccgtgcattc ttgatgcgca
     3841 aagtcagcaa gaaatcccgc tcaatcaggt ccagcctctt acgcccttaa aagccgggga
     3901 taacacactc aaatatcaac ttcgttataa gtccacaaag gcgggagcaa cgggcggtaa
     3961 tgcgacggcg gttctctatt ttgatctggt ttaccagtga aggagtgatg ttgaaacgga
     4021 taatctggat tctgttctta ctgggattaa cggggggctg tgagctattt gcccatgatg
     4081 gcacggtcaa cattagcgga tcgtttcgcc gtaatacatg cgtgctggca caggatagca
     4141 agcaaatcaa tgtgcagtta ggcgatgtct cgctgacacg tttttttcat gggaattatg
     4201 gcccggagaa atctttcatc attaatttgc aggattgcgg aacagatgtc agcacggttg
     4261 atgtcacctt ttcaggaacc cccgacggag tgcagagcga aatgctgtcc attgaaagtg
     4321 gtacggatgc agccagtggg ctggcaattg cgattctgga tgatgcgaag atattgattc
     4381 cgcttaacca ggcgagtaaa gactacagcc tacatagcgg taaggtgcca ttaacttttt
     4441 atgcgcaatt gcgac
//
LOCUS       DAJTUI010000194         4199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTUI010000194 DAJTUI010000000
VERSION     DAJTUI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.196"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..199)
                     /gene="tuf"
                     /locus_tag="OO464_004008"
     CDS             complement(<1..199)
                     /gene="tuf"
                     /locus_tag="OO464_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6228943.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTS"
     gene            complement(314..389)
                     /locus_tag="OO464_004009"
     tRNA            complement(314..389)
                     /locus_tag="OO464_004009"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(354..356),aa:Thr,seq:ggt)
     gene            complement(396..470)
                     /locus_tag="OO464_004010"
     tRNA            complement(396..470)
                     /locus_tag="OO464_004010"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(435..437),aa:Gly,seq:tcc)
     gene            complement(587..671)
                     /locus_tag="OO464_004011"
     tRNA            complement(587..671)
                     /locus_tag="OO464_004011"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(635..637),aa:Tyr,seq:gta)
     gene            complement(680..755)
                     /locus_tag="OO464_004012"
     tRNA            complement(680..755)
                     /locus_tag="OO464_004012"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(720..722),aa:Thr,seq:tgt)
     gene            1117..2067
                     /gene="coaA"
                     /locus_tag="OO464_004013"
                     /gene_synonym="panK"
     CDS             1117..2067
                     /gene="coaA"
                     /locus_tag="OO464_004013"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6228944.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2096..3061)
                     /gene="birA"
                     /locus_tag="OO464_004014"
     CDS             complement(2096..3061)
                     /gene="birA"
                     /locus_tag="OO464_004014"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6228945.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3058..4086)
                     /gene="murB"
                     /locus_tag="OO464_004015"
     CDS             complement(3058..4086)
                     /gene="murB"
                     /locus_tag="OO464_004015"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6228946.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1086 a         1044 c          936 g         1133 t
ORIGIN      
        1 gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg atctggtcga
       61 atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca gcggtcagcg
      121 ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt tttgtacgtt
      181 caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg tgatatcacc
      241 acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga aaaaacaggg
      301 aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc cttaccaagg
      361 gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg gaatcgaacc
      421 cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc atcctagaac
      481 tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt attcgagccg
      541 gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt ggtgggggaa
      601 ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt tggccgctcg
      661 ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac ctactgatta
      721 caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca ttctatggag
      781 acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca cattttatgc
      841 tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca acatcattgt
      901 gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat atctgtcaca
      961 ttgcttttca acgatagctt cctggcagag attttttctt attattcctc cccatctggt
     1021 gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg caaatatcgc
     1081 tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga gcaaacgtta
     1141 atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga ttccgtacct
     1201 atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga tctctcgtta
     1261 gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta tataagctcg
     1321 aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca acgcattcct
     1381 tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc ccgtgtattg
     1441 caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac tacagatggc
     1501 ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa aggcttcccg
     1561 gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc cggcgtgcca
     1621 aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga tggagataaa
     1681 actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca gagcgggatg
     1741 gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt ttcgatatat
     1801 gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct gaaattccgc
     1861 gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt aactaaagaa
     1921 gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa cttaaagcaa
     1981 aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc taatcatgtg
     2041 gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc ctttcttatt
     2101 tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt ccatcctgct
     2161 caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt tctttatcac
     2221 caatgataag tttcactggg cgattaataa aattatccag cttttcccag cgcgacagat
     2281 aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca cgtattagca
     2341 tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg atccacccct
     2401 gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct ccaatgacta
     2461 tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc agcttgcgat
     2521 cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc agctttcgta
     2581 atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc gccgggcctt
     2641 gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa aaccatttcc
     2701 gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg cccgatttaa
     2761 gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc agcacggcta
     2821 cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg atgggctcag
     2881 gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca cgcagtgtct
     2941 gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac tgctcgccag
     3001 agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg ttatccttca
     3061 tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga cttcaggctc
     3121 aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca gttgcaccac
     3181 atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac ggtgcaccgc
     3241 agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac ctgctgccag
     3301 ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg ccagtaatgc
     3361 tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat tgccattcac
     3421 ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata cttgctgtgg
     3481 cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag gttgccactc
     3541 ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt catgtttaaa
     3601 aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt gcttgcctgt
     3661 cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc cataagcacc
     3721 aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat tttccagacc
     3781 aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc cggctcctac
     3841 atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc ggttgatgat
     3901 caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca gaataagaac
     3961 gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt cgtcttcggc
     4021 acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt ttaaggagtg
     4081 gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg tggggggatg
     4141 gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg actgggctt
//
LOCUS       DAJTUI010000195         4188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTUI010000195 DAJTUI010000000
VERSION     DAJTUI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.197"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            84..752
                     /locus_tag="OO464_004016"
     CDS             84..752
                     /locus_tag="OO464_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228947.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="OO464_004017"
     CDS             complement(995..1690)
                     /locus_tag="OO464_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR6228948.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="OO464_004018"
     CDS             complement(1683..3110)
                     /locus_tag="OO464_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR6228949.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="OO464_004019"
     CDS             complement(3121..3840)
                     /locus_tag="OO464_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR6228950.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1115 a         1084 c          866 g         1123 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagt
//
LOCUS       DAJTUI010000196         4130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTUI010000196 DAJTUI010000000
VERSION     DAJTUI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.198"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..104)
                     /locus_tag="OO464_004020"
     CDS             complement(<1..104)
                     /locus_tag="OO464_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6228951.1"
                     /translation="MSRKTRRYSKEFKAEAVRTVLENQLSISEDASRL"
     gene            130..393
                     /locus_tag="OO464_004021"
                     /pseudo
     CDS             130..393
                     /locus_tag="OO464_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            439..648
                     /gene="hha"
                     /locus_tag="OO464_004022"
     CDS             439..648
                     /gene="hha"
                     /locus_tag="OO464_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6228952.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            687..1277
                     /locus_tag="OO464_004023"
     CDS             687..1277
                     /locus_tag="OO464_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6228953.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1517..1777
                     /gene="repA"
                     /locus_tag="OO464_004024"
     CDS             1517..1777
                     /gene="repA"
                     /locus_tag="OO464_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR6228954.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            2001..2075
                     /gene="tap"
                     /locus_tag="OO464_004025"
     CDS             2001..2075
                     /gene="tap"
                     /locus_tag="OO464_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR6228955.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            2068..2925
                     /gene="repA"
                     /locus_tag="OO464_004026"
     CDS             2068..2925
                     /gene="repA"
                     /locus_tag="OO464_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR6228956.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3626..3817
                     /locus_tag="OO464_004027"
     CDS             3626..3817
                     /locus_tag="OO464_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228957.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT         1111 a         1019 c         1021 g          979 t
ORIGIN      
        1 aataatcggg aagcgtcttc actgatcgaa agttgatttt caagaaccgt tctgacagct
       61 tcggctttga actctttaga gtaacgtcgg gtttttctgc tcattattag ctccttctga
      121 tgccattcta tttcaggaag gagtgtccgt taaactcagg ctacctcata ttttcagcga
      181 gatcgttatg gtcgtattct cgggcaggtt tatgcaccag acggaatgaa tattaatcag
      241 tttatggtcc gggccggggc tgcctgggta tatgagcagt acaacacaga tcccgtgcta
      301 cctgtccttc agaacgaagc cagacaacaa aaacgtgggc tctggtcaga tgctgatccg
      361 gttcccccct ggatatggag gcaccgtaaa taaagcgatg gcaatcctat cattcatctg
      421 ttctcagcgg gaataaaaat ggcaaaaaca aaacaggaat ggctgtatca gttacggcga
      481 tgttcatcag tcaatacgct ggaaaaaatt attcataaaa acagagacag tttatcaaac
      541 agtgaacgtg aatcatttaa ttcagcagca gaccaccggc tagcggaatt aatcaccgga
      601 aagttatatg acaggatacc aaaagagata tggaaatatg taaggtaata attcagcaat
      661 aattaattgc caggagcaga taaataatga agttgattat ttttattctc attgttctga
      721 ttattgcagc gctacttatc cggattatcc tcagaagtgt aaatcagcat tctcctttgc
      781 tgatgcaatt gcatgcggca ggtatacgga ctggtgatgc agagcgaata ctgtccagcg
      841 gtgaatactg gcaacgtcag aagacgctgc tgacagaaag ggaagtcagt tttatgaaag
      901 gactgttcag aattgtggat atgaagcggt ggtatctgtg tccgcaggta cgggtagcgg
      961 atatcgtcca gctgaacggg aatatccggc cacgatcacg ccagtggtgg cagttattca
     1021 ggatggtgtc tcagtggcat gttgatgtgg ttatcgttga gctgcgttcg ttcagtattg
     1081 ttgcagcagt agagctggat gatgccagcc atttacgacc ggaacgcaga cgccgggata
     1141 ttcttctgga agaggttctg aggcaggctg gtattccgtt gctcagaagc cacgatgcca
     1201 gaaaactgct gcagatgacc ggagaatggc tgaatacaac aggggctgat cagcagtcac
     1261 cggaacatcg tagctgacgc cttcgcgttg ctcagttgtc caaccccgga aacgggaaaa
     1321 agcaagtttt cctcgctccc ggcgtttcca taactgaaaa ccatactatt tcacagttta
     1381 aatcacatta aacgacagta atccccgttg atttgtgcgc caacacagat cttcgccaca
     1441 attctcaagt cgctgatttc aaaaaactgt agtatcctct gcgaaacgat ccctgtttga
     1501 gtattgagga ggcgagatgt cgcagacaga aaatgcagtg acttcctcat tgagtcaaaa
     1561 gcggtttgtg cgcagaggta agcctatgac tgactctgag aaacaaatgg ccgctgttgc
     1621 aagaaaacgt cttacacaca aagagataaa agtttttgtc aaaaatcctc tgaaagatct
     1681 catggttgag tactgcgaga gagaggggat aacacaggct cagttcgttg agaaaatcat
     1741 caaagatgaa ctgcagagac tggatatact aaagtaaaga ctttactttg tggcgtagca
     1801 tgctagatta ctgatcgttt aaggaatttt atggctggcc acgccataag gtggcaggga
     1861 actggttctg atgtggatgt acaggagcca gaaaagcaaa aaccccgata atcttcttta
     1921 actttggcga gtcagaaaga ttaccggggc ccacttaaac cgtatagcca acaatcaagc
     1981 tatgcgggga gtatagttat atgcccggaa aagttcaaga cttctttctg tgctcgctcc
     2041 ttctgcgcat tgtaagtgca ggatggtgtg actgatcttc aacaaactta ttaccgccag
     2101 gtaaagaacc cgaatccggt gttcacaccc cgtgaaggtg cccggacgct tccgttctgc
     2161 gggaaactga tggaaaaggc ggtgggcttc acctcccgtt ttgatttcgc cattcatgtg
     2221 gcacatgccc gttcgcttgg tctgcgtcgg cgcatgccac cggtactacg tcgccgggcc
     2281 attgatgcgc tgctgcaggg gctgtgtttt cactatgacc cactggccaa ccgcgtccag
     2341 tgttccatca ccacgctggc cattgagtgc ggactggcga cggagtctgc tgccggaaaa
     2401 ctctccatca cccgggccac ccgggccctg acgttccttg cagagctggg actgattacc
     2461 taccagacgg aatatgaccc gcttatcggg tgctacattc cgaccgatat cacgttcaca
     2521 tctgcactgt ttgctgccct cgatgtatca gaggaggcag tggccgccgc gcgccgcagt
     2581 cgtgtggaat gggaaaacag acagcgcaaa aagcaggggc tggataccct gggtatggat
     2641 gaactgatgg cgaaagcctg gcgttttgtt cgtgagcgtt tccgcagtta ccagacagag
     2701 cttaagtccc gtggaatgaa gcgtgcccgt gcgcgtcgtg atgcggacag gcagcgtcag
     2761 gatattgtca ccctggtgaa acggcaactg acgcgtgaaa tctcggaagg gcgcttcact
     2821 gccagtcgtg aggcggtaaa acgtgaagtg gagcgtcgtg tgaaggagcg catgattctg
     2881 tcacgtaacc gtaattacag ccggctggcc acagcttccc cctgaaagtg acctcctcag
     2941 aataatccgg cacgcgccgg aggcattcgc acgcctgaag cccgccagtg cacaaaaaat
     3001 cagcgctaca tacaaaaaac aacctcatca tccaccttca ggtgcatccg gtcccccctg
     3061 tttttgatac aaaacacgcc tcacagacgg ggaattttgc ttatccacat taaactgcaa
     3121 gggacttcac cataaggtta caaccgttca cgtcataaag cgccagacgt caaccctaca
     3181 gggtgcaatg tatcttttaa acacctgttt atatctcctt taaactactt aattacattc
     3241 atttaaaaag aaaacctgtt cactgcctgt cctgtggaca gacaaatatg cacctcccac
     3301 cgcaagcggc gggcccccaa ccgaagccgg tttagttaca acatgcaaac ataaacccct
     3361 aaaaaaccca agtccaacgc aaaactgaaa ccacaacgcc cccctcataa ctgaaaagcg
     3421 ccgccgcccc cgcccaaagg gcgggaacag agtcgctttt aattatgaat gttgtaacta
     3481 catcatcatc gctgtcagtc ttctggctgg acgtatcgaa tacacgctcg taagcggccc
     3541 tcacggcccg ctaacgcgga gatacgcccc gacttcgggt aaaaccctcg tcgggaccac
     3601 tccgaccgcg cacagaagct ctatcatggc tgaaaacggg tatggcttgg cagggctggg
     3661 gatgggtaag gtgaaatctg tcaatcagta ccggctgacg ccgggcttcg gcggttttac
     3721 tccggtatca catgtaacca cagcgtgccg ccttccatgc cgctggcgcg gcatcagaat
     3781 aatacaagca gcatttaatg catttgcaaa agtgtgagta atattaagtg gtcgtttcag
     3841 ggatatcgtt aagacttgag cgataggaat taaaacccca aaaagattaa aaaaacacca
     3901 caaaacggat gtttcttcaa caccactttt gctccatatg aacggaaccg acgattaaac
     3961 tggatggctc tgattgattc agggtatgaa tggcggtttt ttgctccgtt tccctcaaaa
     4021 tggacgcaac ttcccctctg cggctctcag ccgcaccacc gcatccgggc cagcggctca
     4081 tgcatcagga cctgctctgc cagacggtag ccccgcttca gccccgtaaa
//
LOCUS       DAJTUI010000197         4062 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTUI010000197 DAJTUI010000000
VERSION     DAJTUI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.199"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(207..2240)
                     /gene="metG"
                     /locus_tag="OO464_004028"
     CDS             complement(207..2240)
                     /gene="metG"
                     /locus_tag="OO464_004028"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6228958.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2372..3481
                     /gene="apbC"
                     /locus_tag="OO464_004029"
     CDS             2372..3481
                     /gene="apbC"
                     /locus_tag="OO464_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6228959.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3743..4024
                     /locus_tag="OO464_004030"
     CDS             3743..4024
                     /locus_tag="OO464_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR6228960.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT          994 a         1002 c         1046 g         1020 t
ORIGIN      
        1 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
       61 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      121 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      181 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      241 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      301 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      361 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      421 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      481 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      541 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      601 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      661 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      721 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagt
      781 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      841 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
      901 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
      961 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1021 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1081 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1141 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1201 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1261 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1321 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1381 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1441 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1501 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1561 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1621 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1681 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1741 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1801 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1861 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     1921 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     1981 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2041 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2101 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2161 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2221 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2281 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2341 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2401 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2461 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2521 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2581 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2641 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2701 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2761 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2821 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     2881 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     2941 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3001 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3061 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3121 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3181 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3241 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3301 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3361 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3421 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3481 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3541 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3601 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3661 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3721 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3781 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3841 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     3901 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     3961 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4021 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAJTUI010000198         4060 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTUI010000198 DAJTUI010000000
VERSION     DAJTUI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.200"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..236)
                     /gene="flhe"
                     /locus_tag="OO464_004031"
     CDS             complement(<1..236)
                     /gene="flhe"
                     /locus_tag="OO464_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR6228961.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSR"
     gene            complement(236..2313)
                     /gene="flhA"
                     /locus_tag="OO464_004032"
                     /pseudo
     CDS             complement(236..2313)
                     /gene="flhA"
                     /locus_tag="OO464_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2306..3453)
                     /gene="flhB"
                     /locus_tag="OO464_004033"
                     /pseudo
     CDS             complement(2306..3453)
                     /gene="flhB"
                     /locus_tag="OO464_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3689..>4060
                     /locus_tag="OO464_004034"
     CDS             3689..>4060
                     /locus_tag="OO464_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR6228962.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNP"
BASE COUNT         1014 a         1170 c          997 g          879 t
ORIGIN      
        1 cgagattgcg aacacaagcg aacccgcagt cctgcaggtg tcgggccgat aagctgataa
       61 cgccacgcca ccagcgtcat caatcctgaa gcaggttgtc gcgcagaaag aggtgcagac
      121 gacatcgact caccgcgatg atttagcgta attccgacac tacttgcctg ccacatcccc
      181 tccccggcgg cttgcaccag cagcggaaac aataatatcg ctaataaggt tctcatcatt
      241 taccgccaat tgtcgccgtc atgcggatat gtcggttatc agacagttcc agattcgaca
      301 ggaccactaa ctgcggcaag ctgcggcgca ggaagcgaga caataatggt cgcagcgcgt
      361 ggttcaccaa caatactggc ggcgcaccca gcatctcctg acgggataga gcttcctgag
      421 tttgcgccag taaacgatcc gccagccctg gctccagtcc tcccccgccc tgcagcgcct
      481 gtagtaacaa acgttccagc ggtgtatcga ggccaataac atggacttca tctttgccag
      541 gaaaccactg ctgggtaatc gcccgtccca acgccacgcg cacgacggcg cttaattcat
      601 gtggatcgct ttggatgggc gcatgttccg ccagcgtttc gagaatggtg cgcatatcgc
      661 gaatcggtac tttttcatcg aggagatttt gcagcacttt atgcagtgtg gtgagcgtga
      721 cgacgccagg aacgagatct tccgtcagct ttggcatctc ctgggcgacg cgatccaaca
      781 gctgttgcgc ctcctgacga ccaaacagct ctgcggcatg ctggctaatg aggtggttaa
      841 gatgcgttgc taccaccgtg ctggcctcaa ccactgtgta cccctgaatc tgcgcctgtt
      901 cttttagcgc actttcaatc cagatagcat tcaggccaaa tgccggatcg acggtcgcct
      961 caccaggtaa cgtcccggca gcggttccag ggttaatcgc cagccagcgc cccggataag
     1021 catcaccact gccaatctcc acgcctttca tcaaaatgcg atagcgggca ggttgcagat
     1081 ccatattgtc gcgaatgtgc accactggcg gcagaaatcc catctcctgg gcaaatttct
     1141 tgcggatact gcgtatacgg cccaacaact caccatcctg ctggaaatcg accatcggga
     1201 tcagtcgata acccacttcc attcccagag aatcttccag ttgtacatcg ttccacgtcg
     1261 cttcgacaac ggtattattc tctgccattt ttaccggttt gggttcggca ggcgcttttt
     1321 gttcgcgtcc gcgtatccac caggccagcc ccagcaatcc ggcagtgaac agcaaaaata
     1381 ccaggttcgg cattccaggc accaggccga gtaaaccgag cacggcggcg cttaacaaca
     1441 taacgcttgg gttactgaaa agctgattca ccatctgctc gccaacatcc tgatcggtgc
     1501 tgacacgcgt aacgatgccc cccgcggcgg tagaaatcac cagcgccgga atttgtgcca
     1561 ccagaccgtc gccaatggtc aatagcgtat aactttccgc cgcgtgtccc atgctcatgc
     1621 cagttgcagc acgccgacca gcaacccgcc gacaacgtta atgaccatga tgaggatccc
     1681 ggcaatggca tcgccgcgaa caaacttact tgccccgtcc attgagccgt aaaaatcggc
     1741 ttcctgagtc acttcggagc ggcgtttttt cgcctcatct tcaccaatca atccagcgtt
     1801 aaggtcggcg tcaatcgcca tctgcttacc cggcatacca tcgagaacaa agcgcgcacc
     1861 cacttctgct atacgcccgg cacctttggt aatgaccata aagttgatga tcacgagaat
     1921 gacaaacacc acgataccga tagcgaaatt gccaccaacg aggaagtgac cgaacgcttc
     1981 gaccaccttc ccagccgccg ccgcgccggt atgcccttcc atcaaaatga tacgggttga
     2041 agccacgtta agtgccagac gcaacaacat agtaaacaac agaatggtcg gaaacgcagc
     2101 aaactcaagc gtgcgctggg taaacatcgc caccagcaac accatgatcg acaaggcaat
     2161 attgaaggta aacaacaggt cgagtatgaa tgcgggcagt ggcagcacca tcatcgacaa
     2221 gatcaacagg atcaaaatcg gtccggcaag gatctgccat tatgtcgatt tcaggtttgc
     2281 gggcaggcgc agcatcgcgg ccagattact catgggtcgg tttctcgtta ataaaatcca
     2341 gggctgccgg caccggaaga tgagtaggtg tacagggcgc tgtccaccag ccagacgcca
     2401 gcgtttcagt tgccagaccc aggccagcac ttccgccacc gcggcgtaca gttgacccgg
     2461 gatttgttga ccaatctccg catgtcgata cagcgctcgc gccagcggcg gcgcttcaag
     2521 cgtcgggacg ttattttcag cgccaatttc acgaatgcgc agcgcgacca gccctgcacc
     2581 tttagcgacc actttcggtg cgctcatttt gttttcgtca tactgcaacg ctaccgaata
     2641 gtgggtcggg ttattgacaa tgacatccgc tttcggcaca tcggccatca tccgacgccg
     2701 tgcggcagct cgctgcatct gacagatccg tcctttaaca tgggggtcgc cttcgctttg
     2761 tttgaactca tcacgaatat cctgccgcga cattcgcagc tttttcaggt ggctgaagat
     2821 ttggaaaaag acgtcaaatc ccaccattgg aatgacacca agcaccacca gcagtgcgca
     2881 tagccctacc aaatccatcg cattacccat ggcggtaatc ggtgactcgg ccattaagcg
     2941 catcatctgc ggccagtgat gccagagaaa aaaccccgtc acgctgccaa ccaggatggt
     3001 tttcaaaatt gccttaagca actccgcgct agtctgtgcc gagaatatcc gtttaatgcc
     3061 cggtagcggg ttgagtttgg aaaacttcgg ctgcaaggat ttgccgctaa ataccagccc
     3121 ccccagcata accggagaaa tgagcgccac cagcaccacg ccgctaatca gcggtagcag
     3181 cgccagcatg gcttctctga tcagcagaat aatttgcccg aggatcagat tcgggtcttt
     3241 gataatactg tgatcaaaat gcagcccagc ggagagcatg cccgacaatc gacgggccag
     3301 cgacacaccg ccaaaccaga taacactaac gcccaccagc aaaatcagca gtgaggtcag
     3361 ttcacgggaa cgcgggattt gcccctcttc ccgcgctttt tctagtcggt gaggtgtggg
     3421 ggcttctgtt ttgtcgtcgc tctcgtcaga cacgtcgcca atcctggaaa gagtattaag
     3481 cgtaaatgat gccagagcgc aaagcgttca atggtttaag taaggggcaa aacaggcggg
     3541 atttagggcg tttatgcaaa aataggttca gttgcagaac gttttgcgaa ccattttcca
     3601 gttctgatat ttagccattt agcctataga atcgcactcc gcctctctgt tagtttgcta
     3661 ataattaaat tattacaaag gtgatcaaat ggaatggcaa caattgctta actgcaaccg
     3721 tcggaaagat aatctaaaaa aaaccaacat agcattttta catccccttg ctagcggaag
     3781 acaagagatc gagcgggatt acgaccgatt actttttgct gcaccaactc gtcggcttgc
     3841 ggataaaaca caagttttcc cgttagatca aaatgacagc atacggacga ggctgactca
     3901 ttctcacgaa gtagcaaact ttgctcgcgg gataggaatg aggctcgctt tcgagatgaa
     3961 ggagcacatc tttggcgaaa tacccacggg gattgtcgtt gaacgcgacg ttcctgctct
     4021 gttagcagcg atcggactgg cacacgatct cggtaatcca
//
LOCUS       DAJTUI010000199         4056 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTUI010000199 DAJTUI010000000
VERSION     DAJTUI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.201"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(350..997)
                     /gene="ychE"
                     /locus_tag="OO464_004035"
     CDS             complement(350..997)
                     /gene="ychE"
                     /locus_tag="OO464_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6228963.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1474..>4056
                     /gene="adhE"
                     /locus_tag="OO464_004036"
                     /gene_synonym="adhC"
     CDS             1474..>4056
                     /gene="adhE"
                     /locus_tag="OO464_004036"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6228964.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDT"
BASE COUNT         1122 a          994 c          930 g         1010 t
ORIGIN      
        1 tcaattcaat caaatagaga ttggctaagc atgtctatct tttctgatta cagttccagt
       61 agtgaaatgc acaataattt aacaattgat tattatttag cactttcctc taccaaaggc
      121 agtggaatta ctaatatcat ttcaattatt cttcagcaag cccaggatta tgatgtggcg
      181 aaaataacat gatggtgagg agaagcagtt aaggaaatcc ttataactaa ctgtaatttg
      241 agaagaatat ttttacctat tccttttgct tttgataact gcgcgggaac aaagtaaaaa
      301 taatgacgaa gtaaacggag catggcagct ccgtttcatt tgaaaggaat taattaagca
      361 ggccggggaa aatcccctta ataccagtaa cgataaattc aatccccaat gccatcagca
      421 atagccccat aatacgcgta atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc
      481 acggtgccat gcggaacaat ccccagcaac ataaagcgaa caatgcaata gccacaaaga
      541 aaccaaacag atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg
      601 cccctggccc cgccatcaac ggcaacgcca atggcaccac accaatactt tcacgtaccg
      661 cggtttctga tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg
      721 acatcgctat cgtcaccacc aggatgcccc cggcgatacg gaacgaatca attgatatac
      781 caaaaagttg tagaatcgtg tcgccgagaa aaagcgagat ccacaagata atggccacag
      841 acaggttggc tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc
      901 tgataaagac gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt
      961 tgaagtaaac gggaaaatca aaaagggtct gaatcacggt tcgctccgaa gcaaaagccg
     1021 gataatgtta gccataaata aggttgaaaa gacgcgctga caatacgcct tttgacagca
     1081 tttttcacct cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa
     1141 aacatgctaa tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg
     1201 aaaagcgatg ctgaaaggtg tcagctttgc aaaaatttga tttggatcac gtaatcagta
     1261 cccagaagtg agtaatcttg cttacgccac ctggaagtga cgcattagag ataataactc
     1321 taatgtttaa actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt
     1381 tcatcggtta agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt
     1441 actaaaaaag tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt
     1501 aacgcactcg tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag
     1561 caagtagaca aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc
     1621 gcgaaaatgg ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac
     1681 cactttgctt ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg
     1741 tctgaagacg acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt
     1801 atcgttccga ccactaaccc gacttcaact gctatcttca aatcgctgat cagcctgaag
     1861 acccgtaacg ccattatctt ctccccgcac ccgcgtgcaa aagatgcaac caacaaagcg
     1921 gctgatatcg ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg
     1981 atcgatcaac cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg
     2041 atcctcgcga ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct
     2101 atcggtgtag gcgcgggtaa cactccagtt gttattgatg aaactgctga tatcaaacgt
     2161 gcagttgcat ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa
     2221 cagtctgttg ttgttgttga ctctgtttat gacgcagtac gtgaacgttt cgcaacccac
     2281 ggcggctatc tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaat
     2341 ggtgcgctga acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc
     2401 ttctctgtac cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc
     2461 gaaccgttcg cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc
     2521 gaagacgcgg tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct
     2581 tgcctgtaca ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg
     2641 aaaacagctc gtatcctgat taacaccccg gcttctcagg gtggtatcgg tgacctgtat
     2701 aacttcaaac tcgcaccttc cctgactctg ggttgtggtt cctggggtgg taactccatc
     2761 tctgaaaacg ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa
     2821 aacatgttgt ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc
     2881 gcgctggatg aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc
     2941 ctgttcaaca atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa
     3001 actgaagtct tcttcgaagt agaagcggac ccgaccctga gcatcgttcg taaaggtgca
     3061 gaactggcaa actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg
     3121 gacgccgcga agatcatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg
     3181 gcgctgcgct ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa
     3241 gcgaaaatga tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg
     3301 gttgtaactg acgacgctac tggtcagaaa tacccgctgg cggactatgc gctgaccccg
     3361 gatatggcga ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc
     3421 ggtggtctgg acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag
     3481 ttctctgatg gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc
     3541 taccacgaag ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc agcaactatc
     3601 gcgggtatcg catttgcgaa cgccttcctg ggtgtatgtc actcaatggc gcacaaactg
     3661 ggttcccagt tccatattcc gcacggtctg gcaaacgccc tgctgatttg taacgttatt
     3721 cgctacaatg cgaacgacaa cccgaccaag cagactgcat tcagccagta tgaccgtccg
     3781 caggctcgcc gtcgttatgc tgaaattgcc gaccacctgg gtctgagcgc accgggcgac
     3841 cgtactgctg ctaagatcga gaaactgctg gcatggctgg aaacgctgaa agctgaactg
     3901 ggtattccga aatctatccg tgaagctggc gttcaggaag cagacttcct ggcgaacgtg
     3961 gataaactgt ctgaagatgc attcgatgac cagtgcaccg gcgctaaccc gcgttacccg
     4021 ctgatctccg agctgaaaca gattctgctg gatacc
//
LOCUS       DAJTUI010000200         4042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTUI010000200 DAJTUI010000000
VERSION     DAJTUI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.202"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(886..1077)
                     /locus_tag="OO464_004037"
     CDS             complement(886..1077)
                     /locus_tag="OO464_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6228965.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="OO464_004038"
     CDS             1142..1465
                     /locus_tag="OO464_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR6228966.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1407..2165
                     /locus_tag="OO464_004039"
                     /pseudo
     CDS             1407..2165
                     /locus_tag="OO464_004039"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="OO464_004040"
     CDS             2143..2673
                     /locus_tag="OO464_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR6228967.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="OO464_004041"
     CDS             2680..2913
                     /locus_tag="OO464_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6228968.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="OO464_004042"
     CDS             complement(3459..3851)
                     /locus_tag="OO464_004042"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228969.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          917 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAJTUI010000201         3806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTUI010000201 DAJTUI010000000
VERSION     DAJTUI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.203"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            437..2089
                     /locus_tag="OO464_004043"
     CDS             437..2089
                     /locus_tag="OO464_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6228970.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="OO464_004044"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="OO464_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="OO464_004045"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="OO464_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3526)
                     /locus_tag="OO464_004046"
                     /pseudo
     CDS             complement(2596..3526)
                     /locus_tag="OO464_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1052 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gaacagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaacgaag cctttaatat cgacttccac cacccagtca aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgta
//
LOCUS       DAJTUI010000202         3800 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTUI010000202 DAJTUI010000000
VERSION     DAJTUI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.204"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(216..419)
                     /locus_tag="OO464_004047"
     CDS             complement(216..419)
                     /locus_tag="OO464_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228971.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(421..576)
                     /locus_tag="OO464_004048"
     CDS             complement(421..576)
                     /locus_tag="OO464_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR6228972.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(779..1186)
                     /locus_tag="OO464_004049"
     CDS             complement(779..1186)
                     /locus_tag="OO464_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6228973.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1263..1490
                     /locus_tag="OO464_004050"
     CDS             1263..1490
                     /locus_tag="OO464_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6228974.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1474..1896
                     /locus_tag="OO464_004051"
     CDS             1474..1896
                     /locus_tag="OO464_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228975.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1974..2751
                     /locus_tag="OO464_004052"
                     /pseudo
     CDS             1974..2751
                     /locus_tag="OO464_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2758..3504
                     /locus_tag="OO464_004053"
                     /pseudo
     CDS             2758..3504
                     /locus_tag="OO464_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3519..>3800
                     /locus_tag="OO464_004054"
     CDS             3519..>3800
                     /locus_tag="OO464_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6228976.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT          971 a          834 c         1042 g          953 t
ORIGIN      
        1 acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc ccccatggag
       61 atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc ggtgatattt
      121 atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat cgcgcgtctg
      181 tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag acccggtgaa
      241 tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc gccagattcc
      301 agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc cagttgctgg
      361 aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt ctcgttcatt
      421 ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt taaacgtttt
      481 gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca cgccgtacac
      541 cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc cccttaacgc
      601 cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat aaaatttaga
      661 ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt ttgatgtaaa
      721 aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa aaaggggatt
      781 atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg ttgaaatttt
      841 caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc agtgcgtcga
      901 acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta tccgggatcg
      961 gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag cactgaagcg
     1021 cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt tcccattgag
     1081 aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac agttttaatt
     1141 ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta gggaattcta
     1201 atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct aaggagctcg
     1261 atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt gccaatgccg
     1321 caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa ggtcgcgcga
     1381 tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt tatgacgaat
     1441 atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga gcacatcgaa
     1501 tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa cgcaattact
     1561 gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa ggactggaac
     1621 aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca aagggaaaaa
     1681 attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg tcaccgactc
     1741 tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc gttgagtacg
     1801 gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca tttcagcggt
     1861 ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt gccggattgt
     1921 tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa caaatggctt
     1981 caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc aggcttgctg
     2041 agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc tgggcatggg
     2101 cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt acaaaaagtg
     2161 caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag gttggatggc
     2221 tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac ggaaaaagct
     2281 ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg aaacgaaaag
     2341 gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa gaggaagagg
     2401 aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg tctaaaaaat
     2461 tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg catgagtggg
     2521 tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat acgggaactg
     2581 gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag catcgccaat
     2641 gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct ccgacaggtt
     2701 aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta acggaccatg
     2761 aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc gcaacatgta
     2821 accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga aggccagaaa
     2881 cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat tctgaatcgc
     2941 tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt gcagaacgac
     3001 ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat gaccgggtgt
     3061 acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc ggcagctatc
     3121 gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc tgatgttatg
     3181 agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt gcgggagctg
     3241 tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac gaaaaacgag
     3301 caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag cgtggggatg
     3361 ctgacaaacc tgaactatga ggtgatgaaa acattgctcg gcgagcgggt gatggatcgc
     3421 atggtcatga acggcgggcg ctgggttaat tttaactggg agagctggcg tccgaatgtt
     3481 agccattcga gggttgttaa gtagtttcag gaggatttat ggcgaaacct tttactcccg
     3541 aacagcggga agaactgaag acgcgaattg tggaactcgt gcatcaggac ggtcgggtca
     3601 cgattcggca gttgtccgat gaaacaggta tcagtcgtgc gtctgtcggt cgcttatgca
     3661 tagaactggt cgcaagtggt gatgtatata attctggcta cggcttattc ccgtctgaac
     3721 aggctcgcaa ggactggcaa agcgcccgca aaaaactctc gagagtaaag gtgaggaaac
     3781 cggttgttgt tgatccggac
//
LOCUS       DAJTUI010000203         3697 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTUI010000203 DAJTUI010000000
VERSION     DAJTUI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.205"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            11..487
                     /locus_tag="OO464_004055"
     CDS             11..487
                     /locus_tag="OO464_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR6228977.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            550..771
                     /locus_tag="OO464_004056"
     CDS             550..771
                     /locus_tag="OO464_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR6228978.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            934..1308
                     /locus_tag="OO464_004057"
     CDS             934..1308
                     /locus_tag="OO464_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR6228979.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            1355..1729
                     /locus_tag="OO464_004058"
     CDS             1355..1729
                     /locus_tag="OO464_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR6228980.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            1726..2217
                     /locus_tag="OO464_004059"
     CDS             1726..2217
                     /locus_tag="OO464_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228981.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            2229..2426
                     /locus_tag="OO464_004060"
     CDS             2229..2426
                     /locus_tag="OO464_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228982.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            2511..3071
                     /locus_tag="OO464_004061"
                     /pseudo
     CDS             2511..3071
                     /locus_tag="OO464_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            3143..>3697
                     /locus_tag="OO464_004062"
     CDS             3143..>3697
                     /locus_tag="OO464_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR6228983.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHY"
BASE COUNT          908 a         1004 c          915 g          870 t
ORIGIN      
        1 ggcagggaca atgcagcagc tttcctttct gcccggaaac atgacgcccg gcgagcgcag
       61 tctcattctg cgggccctgc aaaccctgga ccgccatctt catgaacccg gcgtggcctt
      121 cacctccacc cgtgcggcac gggaatggct gattctgaac atggcgggac tggagcggga
      181 agagttccgg gtgctgtatc tgaacaacca gaatcagctg attgccggtg aaaccctctt
      241 caccggcacc atcaaccgta cggaagttca tccccgggaa gtgattaaac gcgccctgta
      301 ccacaatgcc gctgccgtgg tgctggcgca caatcacccg tccggtgaag tcacacccag
      361 taaggcagac cggcttatca ccgaacgtct ggtacaggca ctgggcctgg tggatatccg
      421 ggtgccggac catctgatag tcggtggcaa ccaggttttc tcctttgccg aacatggtct
      481 gctttaaccc gtcacaacca catcacacct gttttcactt ttatcttctg tcttcagagg
      541 tatcacatca tgagaatcat cacccgtggt gaagccatgc gtattcacca acagcatcct
      601 gcatcccgtc tttttccgtt ctgtaccggt aagtatcgct ggcacggcag tgctgaagcg
      661 tatactggtc gtgaagtgca ggatattccc ggcgttcttg ccgtgtttgc agaacgccgt
      721 aaggacagtt ttggcccgta tgtccggctg atgagtgtca ccctgaactg atgttccgtc
      781 acccgggaaa cgagtacgac cagatgacct cagacgccgc tgtggatgag gccattctca
      841 tcaatgcata catcttcacc gaaaccggtc agcgcgccgg ctgacctgtc atcgttaaac
      901 cattcttaac cacttttcag agaggatttt atcgtgtcag acaaactctc cgggataact
      961 catcccgatg acaatcacga ccgcccctgg tgggggcttc cctgcacagt gaggccctgt
     1021 tttggcgctc gtctggtgca agagggtaac cgcctgtatt accttgccga ccgcgccggt
     1081 atcagaggcc gcttcagcga cgcagatgca taccatctgg accaggcctt tccgctgctg
     1141 atgaaacaac tggaactcat gctcaccagc ggtgaactga atccccgata tcagcatacc
     1201 gtcacgctgt atgcaaaagg gctgacctgc gaagccgaca ccctcggctc ctgtggttac
     1261 gtttatctgg ctgtttatcc gacaccagca cccgcaacca cctcataacc ccgaagctct
     1321 cacctttagc actaccagtt acagagaact taagatgaaa acattatccg acacacatgt
     1381 acgggaggta tctcgctgcc cgtctcccgt caccatctgg cagacactgc tcatccgact
     1441 gctggaccag cattatggcc tcacactgaa tgacacaccg tttgtcgatg aacgtgtgat
     1501 tgagcagcat attgaggcag gcatttcact gtgtgatgca gttaattttc tcgtggaaaa
     1561 atacgcgctg gtgcgtaccg accagccggg attcagcacc tgtccccgct ctcagttaat
     1621 aaacagtatt gatatcctcc gggctcgccg ggcgaccggc ctgatgaccc gcgacaatta
     1681 cagaacggta aataacatta ccctgggtaa gtatccggag gcgaaatgat gaaactggcc
     1741 ctgacactgg aagccgacag cgttaacgta caggcactga acatagggcg cattgtcgtt
     1801 gacgtcgatg gtattgagct cgctgaactg attaacatgg tctgcgataa cggctactcc
     1861 cttcgtgttg ttgatgaatc tgaccagacc tcagcagaat gcacgccacc atttgctacc
     1921 cttaccggca tacgctgcag taccgcacat atcacggaaa cggacaacgc ctggttgtac
     1981 tcgctgtcac accagaccag tgacttcggt gaatcagaat ggattcattt tacaggtagc
     2041 ggatatctgt tacgtaccga tgcgtggtca tacccggttc tgcggcttaa acgcctgggg
     2101 ctgtcaaaaa cgttccgtcg tctggtcgtc acactcatcc ggcgttatgg cgtcagtctc
     2161 attcatctgg atgccagcgc tgaatgcctg ccgggtttac ccactttcga ctggtaacca
     2221 ggaacaacat gaaatcatta accacggaaa cagcgctgga tattctgatt gcgtggctgc
     2281 aggacaatat cgactgcgga tcgggaatta tctttgacaa cgatgaggat aaaacggatt
     2341 cagcagcact gttgccctgt atcgaacagg cccgggagga tgtccgtacc ctgcgtcatc
     2401 tgcagcttct gcaccagaac cggtgaagct cactcaccag acttcattcc tctgacgcca
     2461 gcctgcacag ggctggcgtt ttcatttatc tgcaaaaagg aatatcgatt atgtcagata
     2521 tcacaatctc ccgtccggaa gtggtcaacg ggcatacgga cgttatctgc tcaaccagca
     2581 ttcgccacat tctggctgta cgaaagagta cactgttgca aatcgacaca cttatccgac
     2641 aactggctga aatctcggtg ctgacggaaa gcatcggagg taaaactgcc ccggactggg
     2701 ccatgaaaca ggattttcgc tgcggctgct ggctgatgga gaaacccgaa acagcaatga
     2761 aagccattac ccggaatctt gatcgcgaaa tctggcgtga cctgatgcaa cgttccggga
     2821 tgctgtcatt aatggatacg caggcccgtg atacatggta ccggtcactg gagtacgata
     2881 attttccgga aatcagcgaa gcgaacattc tgagcacatt tgaacaactg caccagaata
     2941 aggatgaggt gtttgagcga ggggtgatca acgtcttcag agggctgaac tggaattaca
     3001 aaaccaattg cccctgtaaa tttggcagta aaattatcgt caacaatctg gtgagatggg
     3061 accggtgggg ataatgatat ggtcaccccc accttaacag cagtacgctg ttaaggtaaa
     3121 tccctttaac ggagagacca tcatgcagaa catcacactt attggtatcg atctcggaaa
     3181 gcactccttc cacattcatt gtcaagacaa gtctggtaag gctcttctgc gtaaaaaatt
     3241 tacccgtact aaactgatgc agtttctggc gacatgtccg ccctcagttg ttgtaatgga
     3301 agcctgtgcg ggtgcacact ttatggctcg ctgtatctct gattttgggc atgaaactaa
     3361 gctgatttct ccacagttcg ttcgcccgtt tgtcaaaagt aataagaatg attttgttga
     3421 tgcagaagcc atatgcgaag cggcttcacg accatcaatg cgttttgtcc agccaagaac
     3481 tgagacccag caggccatga gggcgttaca ccgggtcaga gaatcgctca tcagagataa
     3541 ggtgaaaaca accaaccaga tccacggttt cttgctggaa tttggaatca gcctgccgat
     3601 aggtgatgct gtaataaaac ggttgtctct cgtgttggct gaacacgaaa tccccgaata
     3661 tctgagccgc cttttggtga ggttgcatac ccactac
//
LOCUS       DAJTUI010000204         3678 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTUI010000204 DAJTUI010000000
VERSION     DAJTUI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.206"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            58..2055
                     /gene="betT"
                     /locus_tag="OO464_004063"
     CDS             58..2055
                     /gene="betT"
                     /locus_tag="OO464_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCR6228984.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2118..3395
                     /locus_tag="OO464_004064"
     CDS             2118..3395
                     /locus_tag="OO464_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR6228985.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          890 a          835 c          929 g         1024 t
ORIGIN      
        1 tttgtgagag gtagtacctt ctatccagtg cgaatttaat taatggaata aatgattatg
       61 agtgaaaatg atacaatccc aaagaagtct acaagtcaga ttaacaaagc ggtattcttt
      121 acatctgctt tgctaatttt ccttcttgtc gcctttgccg ccgtattccc ggatgtcgcc
      181 gacaaaaatt ttaaactact tcagcaacaa atcttcacga atgccagctg gttctacatc
      241 cttgctgtgg ccctgatttt actgagtgtc acgttccttg gactctcacg ctacggtgat
      301 atcaagctgg gcccggacca tgcgcagcct gatttcagct accactcctg gtttgcgatg
      361 cttttttcgg cagggatggg gatcggcctg atgttctttg gcgttgccga acctgtaatg
      421 cattatcttt cgccacccgt tggcactcca gaaaccgttg cggcagctaa ggaagcaatg
      481 cgtctgacct ttttccactg gggactgcac gcatgggcaa tttatgccat tgtggcgctg
      541 attctggcgt tcttcagtta ccgtcacggt ctgcctttaa ctctgcgctc cgcactctat
      601 cccattattg gcgatcgcat atacggacct gtaggacatg cggttgatat tttcgctgtt
      661 ataggcacgg tctttggcgt tgcgacatca ctgggttacg gtgttttgca ggtgaatgcc
      721 ggtttgaacc atcttttcgg ggtgcccatc aatgaaacgg tgcaggtaat tctgatcgtg
      781 gtcatcacgg ggttagcgac gatttcagtg gtgtccggtc tggataaggg aatacgtatc
      841 ctgtctgaac tcaatctggg tctggctttg ttgctcctgg cgctggtcct gtgtctggga
      901 ccaaccgtgc ttctgctgaa gtcatttgtg gaaaatacgg gcggttatct ttcggaactg
      961 gtgagtaaaa cgttcaacct ttacgcgtat gagcccaagt cgagcaactg gctggggggc
     1021 tggacattac tgtactgggg atggtggctt tcatggtcgc cgtttgtggg gatgttcatc
     1081 gcacgggtct cccgcgggcg aaccattcgc gagtttgtca ccggcgtgct gtttgttccc
     1141 gcgggtttta cgctaatgtg gatgacggtg tttggtaaca gcgcgatcta tctcattatg
     1201 aaccaggggg ccacagacct cgccaatact gttcagcagg atgtggcgct ggccctgttt
     1261 aatttcctgg agcatttccc gttctcttct gtgctgtcat tcattgcaat ggcgatggtc
     1321 atcgtcttct ttgtaacgtc tgctgattcg ggggcaatgg ttgtggatac tctggcatca
     1381 ggtggagtgg caaacacacc cgtctggcag cgaatattct gggcctcgct catgggcatt
     1441 gttgcaattg cgcttctcct tgccggaggg ctaagtgcgc tgcaaacggt gacaatagcg
     1501 agtgcattgc ccttctcagt gatcttacta atatctatat acggactttt aaaagctttg
     1561 cgccgggatt tgaccaagcg tgaaagcctg agcatggcga caattgctcc tacggctgca
     1621 cgtaacccaa ttccttggca gagaaggtta cgcaatatcg cgtatctgcc gaagcgatct
     1681 cttgtgaaac gttttatgga cgacgttatc cagcctgcca tgacgctggt tcaggaggaa
     1741 ctgaacaagc aggggacgat aagccacatt agtgatgcag tcgacgatcg tattcgtctt
     1801 gaagtcgatt tgggcaacga gctgaatttt atatatgaag tgaggcttcg cgggtatatc
     1861 tcaccgacct tcgcgctcgc cgcaatggat aatgatgagc agcagactga acaacatcga
     1921 tattatcgcg ctgaggttta tctcaaagaa ggcggtcaaa attatgatgt gatgggctgg
     1981 aaccaggaac agctgattaa tgacatactg gaccagtacg aaaaacacct gcacttcctg
     2041 cacctggttc gttaatagca acatgccgtc ctgggggcgg caattattat cccggccgca
     2101 atatgaggga atgcagaatg atttcacgct ggaaatggat gctgaagcag acaattaaaa
     2161 aactatggtt cagggcaacg ttattcgcaa ttgtcgcgat aataacggcc cttttatcaa
     2221 ttctttttaa atcaatgata cctgagtcgg tttccgtgaa ggttggtgcg gaagcagtcg
     2281 ataacattct gaacatactg gcatcgagta tgctggcagt gaccacattt tcgctgagta
     2341 tcatggtcac agcctacggt tcagccacta ctaatgtgac tcccagagct acgcgtttag
     2401 ttgttgaaga cgtcaccaca caaaatgtac tggccacctt catcggttct tttctcttca
     2461 gtctggtagg gattattgcc ctcaatatgg gagcttatgg agaaaggggg agagtcattt
     2521 tattcattgt cacactggtt gtcattgcct taatcctcat cacattgctt cgctggatac
     2581 agcatttgac ctctctgggg agggttggtg agacaacggc acaagtagaa caggcggcca
     2641 tcgaaacatt tattgcgaga gcaagaaatc cctgtctcgg tggatatcca tggcttgaga
     2701 acaatgaaca gccgaaagga acggttgcag tttatccgaa gaagattggc tatgttgaat
     2761 atattgatat ggtgaaactt agcaagctgc tgaccaatga tccccgtcat gtatacctcg
     2821 tggcgcagcc aggcagtttc atacatccgt ccatgccagt tttgtacctg agtcagggcc
     2881 aggagtcgtc aatcaacacc gatttacttg agacgattat tgtctcggat gcacgttcat
     2941 ttgctcagga tcctcgattt tgtcttagcg tcatggccga aatagcctgc cgggcccttt
     3001 cccctgcagt gaacgatcct ggaaccgcca ttgatgtcat tggcagaggt gttcgtatac
     3061 tttccactta cgcgcagaat aaatctgatg aaatagaagt gaaatatcct tcagtacatg
     3121 ttgcaccact tcagaataac gatctactgg aagacttttt ctcacctgtc gcgcgcgatg
     3181 gtgccagtat gagggagatt cagataagag tccttaaagg actgtcgatg ctgagtaaag
     3241 gttggcctga gatatttgct gaagccgcac ataccctggc atttgaaaca ttagagcatg
     3301 caactcgtgc tgaccatata gattctgata gatatctaat aaaatcaact tattataatt
     3361 tatttagtgg cgaatattct aataaaaaaa catagctgca gagcaagcaa ttcggttgtt
     3421 ggcaagtcaa gggcaaagca ttcgcgtgag aatgggcgta tgctttgtgc caaaagcaaa
     3481 cgttgctaat tctgcactgt tcagcgatgg cgcagtagag acaggcactg ccactgtcca
     3541 gtcggcaaaa gcgaacactt tgccactgcg gggataaact gattgaagct ctcgcgcaaa
     3601 atcacttatt cttttgcgtt aataaaatgt aggagatggg tcattgatcc tacccacgta
     3661 atatggacac aggcctaa
//
LOCUS       DAJTUI010000205         3606 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTUI010000205 DAJTUI010000000
VERSION     DAJTUI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.207"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            19..897
                     /locus_tag="OO464_004065"
     CDS             19..897
                     /locus_tag="OO464_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6228986.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="OO464_004066"
     CDS             894..2285
                     /locus_tag="OO464_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6228987.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="OO464_004067"
     CDS             2282..2539
                     /locus_tag="OO464_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228988.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="OO464_004068"
     CDS             2634..2834
                     /locus_tag="OO464_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR6228989.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="OO464_004069"
     CDS             2827..3207
                     /locus_tag="OO464_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6228990.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            3407..3483
                     /locus_tag="OO464_004070"
     tRNA            3407..3483
                     /locus_tag="OO464_004070"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3441..3443,aa:Ile,seq:cat)
     gene            3485..3561
                     /locus_tag="OO464_004071"
     tRNA            3485..3561
                     /locus_tag="OO464_004071"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3519..3521,aa:Arg,seq:tct)
BASE COUNT          943 a          793 c         1041 g          829 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaacac gctggtggtc
     3361 agatgagttt gcagatgtga tgacatatgg ttattattct gcctccggcc ctttagctca
     3421 gttggtcaga gcgagcgact cataatcgcc aggtcgctgg ttcaagtcca gcaagggcca
     3481 ccaaccacca ctagctcatc cggatagagc atcaaccttc taagttgacg gtgcgaggtt
     3541 cgagtcctcg gtggtgggcc agcgccgact tagctcagca ggcagagcaa ctgacttgta
     3601 atcagt
//
LOCUS       DAJTUI010000206         3488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTUI010000206 DAJTUI010000000
VERSION     DAJTUI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.209"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..653
                     /locus_tag="OO464_004072"
                     /pseudo
     CDS             1..653
                     /locus_tag="OO464_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            650..1036
                     /locus_tag="OO464_004073"
     CDS             650..1036
                     /locus_tag="OO464_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6228991.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1061..3274
                     /gene="tssI"
                     /locus_tag="OO464_004074"
     CDS             1061..3274
                     /gene="tssI"
                     /locus_tag="OO464_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6228992.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTHDVSE"
     gene            3297..>3488
                     /locus_tag="OO464_004075"
     CDS             3297..>3488
                     /locus_tag="OO464_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HCR6228993.1"
                     /translation="MTSLYRIQEGCFALPETFLDRTVNIFVPSGNERATPSLNIFRDT
                     LRPDENLTTYIDRQIALMKK"
BASE COUNT          954 a          744 c          917 g          873 t
ORIGIN      
        1 aaaaaacaaa tattgatggc aatatcatta tacataagga tggtaggagg acgtatttat
       61 taaatcaaaa tactggttcg attacggctg tgataagatg gaagtatgag tagtataagg
      121 ataataccgt tacagatgac tggactatat gggaaaaagc tatttttatg cgagcgatat
      181 atttatctgt tcagcaggca tggaatggga aaataactta ttctgtttcc ggtgaatctg
      241 agttcgctaa aaaatttcag ggtaaagctt tgccttttga tgtaagaata atttctgcat
      301 ctcagaatga agactggttg gttattgcaa caaaggtttt gcctggtgct gatttaagaa
      361 catatgttga ttttaaaaac agtacggttc atgttgattc agcgggcctg gaaaaagtag
      421 cgaaatgtat taactgtaat aatacgttgc aagtaaatat acctcatgaa gccggacatg
      481 tacttggtta cttagatgat gattatgatt cttcgtctcc gtatgttgga gatattagtg
      541 ggttgatgaa tgtgggaatg gagctttggg aacgatacct taaaaacgca actataacgt
      601 tgaatatcat aatgcctgaa actaaattta ctttattgaa tgtaaccaaa tgaaattttt
      661 ctgtattata gtgattttcc tttcaataaa ttacatagct atagtgaatg ttttcaagtc
      721 ttctgcagtt gctcaatcca ccaaatatta tgaagaaaat gatatacatg atggaagtaa
      781 tgacatcttt tttatgaaga aatatgggcc gcctgctcat gagttcgtga ggccacgata
      841 taaatttgtt ggtgagtatt atatcggctt gaatcaaact tatcgcataa cagatcctga
      901 atttcaagat gttcctatta aggaaatgtt ctggaatttg cagggggatt tgaatctgac
      961 atgttggttt cattataaag acgggcaatg gagagtgatt acttatattt tttggccacc
     1021 aggggctaga ttttaatccg gtattaaagg agcaaatatc atgagttttg tatctacggg
     1081 taataaacca gttggaaacg gtggacccgt catcaccaca ccaccaatag ctggcgaaag
     1141 tggcggtatg agcacaggtt cagctgtaac cgatgtatct ggtgccgccg aggagatggc
     1201 agaacaggct gcggcggacc tgtttggtgc gctgccggaa ccgtcaggtc tggttaaagc
     1261 cgcagtagct gctgcgcagg ctgccgctgc agcagcaggg atctcggata tggctggtgc
     1321 agttcaggat gcagcggcgt cgcttgcagc cggtgctccg ggggcgcata acgtcacagt
     1381 aagtggtagt gcggtgccgc cgcagatgct tttgtttgca ggcatgaatg gttcggaagg
     1441 gcttgggaat ctgttttctt acacagtaca gcttaaaacg ctggacgccc tgaacctggg
     1501 gtacgtttcc cccgctgcta acctgccgct tcagctcatg gtgggcaaag acctgtgcgt
     1561 cagtatcgaa ctggacggcg gcggaaaacg gtatatcagc gggctggtga cggcggcgag
     1621 ggtggtcggc catgagagcc gttcggtaac gtatgaatta cgcattgagc catggctgaa
     1681 gctgctgacc cataccagcg actacaaagc gttccagaac aaaaccgtgg tggatattct
     1741 cgatgaagta ctggatgaat attcctttcc ggtggaaaag cggctggtgg aaaattatcc
     1801 accccgcgcc tggcaggtgc agtatggtga aaccgacttc gatttcatcc agcgcctgat
     1861 gcaggaatgg gggatttact ggtggtttga gcacagcgag aacagccaca cgctggtgct
     1921 ggtggatgcc atcaatggtc ataaagcgtg ccctgactcg ccgctggtgg agtggcacca
     1981 ggaagggctg aaactggata aggaatttat ccacaccatt acggccagtg agaggctgcg
     2041 tactggaaag tgggtgatgg acgattttga ttttatgaag cctcgttcgc tgcttaaaag
     2101 taccgtggcc agtccgcgtg ataccggtca tgctgaatac gagcattacg aatggcctgg
     2161 tgattacttc actacgggcg aaggcgagat gctgacgcgc attcgtatgg aagcgcagcg
     2221 cagcccaggc agccgggcac atggggccgg tcatatccgt accctcatga ccgggtacac
     2281 ctttacgttg atgaatcacc cgacggcaga aattaatcag gaatatctgc tggtacagac
     2341 cactctgttt ttacgggata atgcgcagca cagcggtcag gatcagcatt ttacctatgt
     2401 caccacgttt gaactgcacc ccaccagcga agtttaccgc ccacaacgaa ccctgagcaa
     2461 accccacacc aaaggcccgc agagcgccat cgtcaccggc ccggcggggc aggaaatctg
     2521 gacggataaa tatggccggg tgaaggtgca gtttggctgg gaccgttacg gaaagaatga
     2581 cgaaaacagt tcctgctgga tccgcgtcag ctatccgtgg gcgggtaaag gattcggcat
     2641 gatccagatc ccgcgtattg gccaggaagt actggtggat ttcaaaaacg gcgatccgga
     2701 tttgcccatc atcgtggggc gcacctataa ccaggacacc atgccgccgt gggggctgcc
     2761 gggcatggcg tcgcagagtg ggatattcag ccactcattg cagggagggc cgactaacgg
     2821 aaatatgctg cgcttcgatg ataaaacggg tgcggaagag gtgaagttcc atgcggaaaa
     2881 agacctcaat accacggtaa aaaacaatga aacccacacg gtcatggtcg atcgcataaa
     2941 aaccattgtc aaaaatgaaa ccatcagcgt gggagaagac cgtaatgcca ccatcacaaa
     3001 aaatgatggt ctttccgtca aactggctca aaccatcaat gtcggaacga catatcgtct
     3061 ggatgttggc gatcaattta cccttcgttg cggcaatgca gcgcttgtat tacacaagga
     3121 tggctcgatt gagttttgcg gaaaacaact gttactgcac accagcgatg ttatgcaact
     3181 cattggcagt ggcattgata tgaatccgga tggcggcaca gccatgactg cggatgatat
     3241 tgccccgtta cctacccacg acgtttctga ataaaaaata taaaaggaat ttttctatga
     3301 ccagccttta ccgtattcag gagggctgct ttgccctgcc tgaaactttt cttgatcgca
     3361 ccgtcaatat cttcgtccca tcgggcaatg aaagggcaac tcccagtctg aatattttcc
     3421 gcgatacgct caggccagat gaaaatctga ccacctatat tgaccgccag atagccttga
     3481 tgaaaaaa
//
LOCUS       DAJTUI010000207         3483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTUI010000207 DAJTUI010000000
VERSION     DAJTUI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.210"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..311
                     /locus_tag="OO464_004076"
     CDS             <1..311
                     /locus_tag="OO464_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6228994.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="OO464_004077"
     CDS             468..1217
                     /locus_tag="OO464_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR6228995.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="OO464_004078"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="OO464_004078"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR6228996.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OO464_004079"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OO464_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6228997.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..>3483
                     /locus_tag="OO464_004080"
     CDS             3361..>3483
                     /locus_tag="OO464_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR6228998.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEE"
BASE COUNT          957 a          906 c          862 g          758 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gag
//
LOCUS       DAJTUI010000208         3318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTUI010000208 DAJTUI010000000
VERSION     DAJTUI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.211"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            445..1272
                     /locus_tag="OO464_004081"
     CDS             445..1272
                     /locus_tag="OO464_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6228999.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1316..2065
                     /locus_tag="OO464_004082"
     CDS             1316..2065
                     /locus_tag="OO464_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6229000.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2062..2631
                     /locus_tag="OO464_004083"
     CDS             2062..2631
                     /locus_tag="OO464_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229001.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2755..2997
                     /locus_tag="OO464_004084"
     CDS             2755..2997
                     /locus_tag="OO464_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6229002.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3001..3147
                     /locus_tag="OO464_004085"
     CDS             3001..3147
                     /locus_tag="OO464_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229003.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3156..>3318
                     /gene="xisR"
                     /locus_tag="OO464_004086"
     CDS             3156..>3318
                     /gene="xisR"
                     /locus_tag="OO464_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR6229004.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          926 a          792 c          853 g          747 t
ORIGIN      
        1 tgacaagcgt tttatcttca acgggaatga gatatggaaa cgtggagaat caaccacaga
       61 gtatacccgc gcacaccagc gaacaatcag acgactgcgc agactcatcg ccaggaaaca
      121 gtatacagaa aaatggttca gaaaatatga cacatggagc gccggatatt acgaactgat
      181 ggcaacaaaa ccattctgac gtaaacgaaa ttaaccatga cgcaattaaa taaggcaagc
      241 cgaatacatc aggaggacca tgaacattta tttcagaata gttatatcac tggcaattat
      301 cgcatgtatt tacggattaa tcgttccggc cctcatatca atgaaggata cggtagcagt
      361 gatttctggc tttgctctgg cgtgtctgac cccgccctgc atttatgcca tttataaggg
      421 tctttctttt tctaaggata aaagatgaaa aaagttattc ctgctttagc ccttgttctg
      481 ctgaccaccg gacttgtcgg ttgcgatcgc gttgagcctg gtaatgttgg catcaaggta
      541 aacaaactgg gcgacgataa aggcgtcgga gaagtggtcg gtgttgggcg ctactggacg
      601 ggatggaaca ctgaggttta catcttcccg accttcaaac aaatgaagac ctacgatgag
      661 ccattcagct tccagatgag tgacggtaca accatcggct atcacatcgg cgtggcctac
      721 aaagttgatc catccaaagt taccacagtc tttcagacct accgcaaagg cgtggacgac
      781 attaccgaca ccgacctgcg ccagaagata gccgacgcac tcaaccgact ggccagcaaa
      841 atgaccaccg acaaatttat cgacggcggc aagtctgagc tgctggatgc agctcttaaa
      901 gacattcagg aagaaatgac gcccatcggt attcaggtaa tgagcctctc atatgtgggt
      961 aagccggagt acccgcctac tgttatcgac agcattaatg ccaaagtcac ggcgaaccag
     1021 aaaaccctgc aatgcgagca ggaagtaaaa cagcgcgaag cggaagccaa catgttgcgc
     1081 gcggaagctg ccggacaggc agatgcgatt cgcacaaaag cccaggccga agccgatgct
     1141 attcgtttac gcggtgaagc tctgcgccag aacccaaacg ttatggagct ggaagccatc
     1201 aacaagtgga acggtacact gccgcaatac atgaccagcg gtgccaacac accatttatt
     1261 caggttaagt aatacatatg cccggtatta cacgccgggc tgtctggaga taaaaatgaa
     1321 tactgtaacc atcaacaaca aacagtttcc ggtaatcgaa tatcgcggtc agcgtgttgt
     1381 gacgctggcg atgattgatg aagtccacca gcgaccggaa ggaaccgcgc gtgcagcatt
     1441 caaccgaaac cgtgagcatt tcatcagcgg tgtggattat gccgaattag gtgcggacgt
     1501 aatacgtacg gacctcccgg aaggaacatt ctctaaattt gcaccatcgg ggattgtact
     1561 ttttgaatca ggttacctga tgttgacgaa gccattcaat gacgcccttg cctggcaggt
     1621 tcagcgcgaa ctggttaaca gctacttccg cactcgcaca ccgctaacgg aaatcgagat
     1681 gatcgctgcg atagccgccg acgctgttcg tcaacagaag cgcctgaatc atgtggaaga
     1741 acagctcgaa acagtcacag aagcggtgga aaccatcaaa cgcggaaata tgcgcgccgg
     1801 atatgtcggt taccgccagg tggtagccaa aagtggaatg agtgacacca agtgtcggaa
     1861 tctggtgaac gcatatcaaa ttcctacaga cacccacgaa ttcatgactc cggacggtct
     1921 gttgtctcgc agggctatcg tcgagcttga gccatttatg gcggcgttcc accaaatgat
     1981 gtcagaggct gacccacgcg gcacacgctg gtatcacccg aagatggggc ttttccaggt
     2041 gatcggatgg gaggataaag catgatcatc cagtcaaaac ttattcgcgc cgctctggtg
     2101 tgcgctgcta aaaacgacgt tcgttattac ctgaacggtc ttcacatcac gccgaaacat
     2161 attgaggcaa ccaatggttc cgtggcactg cgcatggctc acggcatccg gacgaagaaa
     2221 aacatcattg tccagttcga agggggcgtc ccggccaaag ccgaaacgac agagctgatt
     2281 tttagtaaag agccgatcgc tgttcatcgc gaccagtttc agcgacgact gtccattacc
     2341 ggcattaaac tggtggacgg ttgttttccg gatttggatc gcatcattcc gaaaaagttt
     2401 gaccgctgta ctcacccggt gctccaggcg ggttacctga gttatccaga gaagatgttt
     2461 ggtcgtgagc gtaaatttat tcccgtccag ttacgtccct ccggtgacgg gcaagcggtc
     2521 agaattcagt ttgattccat catcaactca atgtatggca atcctgaatt tgttgtgatg
     2581 ccttgtcgtg atcatggcga tttcaacgtg gctcaggagc atccggaatg aaaatcgaat
     2641 accaggacgc cgcaggaggt gaatcaatga gctggcctga tgcaatcgta actctgggga
     2701 tggtattcgc agtagcgttt gttgtgtact cgatttgtcg atggggataa ccacatgttc
     2761 gctttgattc aacgcgggca gatatacaca gacagagccg gatacccagt agaaattact
     2821 cgcagtactg agcactcagt gttctttcgg aggatggacg gacgtaccgg gcgagtacgc
     2881 atcggtgaat tcagtagcct gttcgagcat attgaccacc tggaatatca caagattctg
     2941 gctgaaacag aacaagaaaa gcacctgaag aaattacgcg caatgcaaag gaagtaaaaa
     3001 atgaataaag cgtttgagct atgggtccac cagcgttacg gcaaccacta cgacctgacg
     3061 cgagatgttg acggtttcta ctgtcgtgaa attgtgaaac gaatgtttga ggtctggtgc
     3121 cactgccgtg ggctgagtgt tgtgtgaggt aatgcatggg caatgtgatt caactggctc
     3181 ccaatgaatg ggtttgtgaa agcgttctaa tcgcaattac cgggctcaaa ccaggcacaa
     3241 ttcttcggac ccggaaagaa tgctggatgg ttggaagaga gtatattcac gtatcaccag
     3301 acggtaatcc aaagcctt
//
LOCUS       DAJTUI010000209         3258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTUI010000209 DAJTUI010000000
VERSION     DAJTUI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.212"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..442
                     /locus_tag="OO464_004087"
                     /pseudo
     CDS             1..442
                     /locus_tag="OO464_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1026..1742
                     /locus_tag="OO464_004088"
     CDS             1026..1742
                     /locus_tag="OO464_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229005.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2294..2698
                     /locus_tag="OO464_004089"
     CDS             2294..2698
                     /locus_tag="OO464_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229006.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2761..3258
                     /locus_tag="OO464_004090"
                     /pseudo
     CDS             2761..3258
                     /locus_tag="OO464_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          909 a          675 c          749 g          925 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct
      301 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      361 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      421 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      481 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      541 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      601 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      661 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
      721 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
      781 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
      841 ccaactagcg ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc
      901 attctgcaga gtattttgat atgataaata tgcattaaca atgatattat tgttaatttc
      961 ctagcatgaa aattaaaaga tcaactttta tctcgaatat tttttacatt atcagttagt
     1021 ttcttatgaa tgacaattca ttgcttagaa attcttcatt attcatcgcc tatatgggat
     1081 gtgtagggtg ggtcagtgct tattcttatg gctggggaac ctctttttac tatggatttc
     1141 catggtgggt tgttggtgca ggtttggatg atgttgccag aagtttactc tatgctatca
     1201 tagtaatggg tatacttttt accggttggg ggattggtat tttatttttt cttcttataa
     1261 agaaaagaag taaaatacaa gacttgagtt tttttagact attttttgct attaccctac
     1321 tgtttttccc tgtaatattc gaacttttaa ttctcaaaca atatttcata cttccattat
     1381 cactttcatt cattattagt tcattggtta tttcaataat aattcgtata tatggacgga
     1441 tcttttctgt atcatgtttt tcagatatcc cttttgttag agaacaccgc attaagttaa
     1501 ttatggctgg ttttttggtt tatttttggt ttttttcctt tcttgttggc tggtataaac
     1561 ctcaattaaa aaaagaatat cagatgcttt gttataataa tagttggtat tatgttcttg
     1621 ctcgttatga ttcacggctc gttttatcta gttcatttaa agatgacagt aacagattct
     1681 taatatttaa tacagaacag tcgggatttt atgaaatcaa tgatgtttat gtcagaaagt
     1741 aataatcctc tcccacaaag tgaattaaat gaatatataa tgcgttcgaa gtatatgtat
     1801 tgagctgtca tactgagaaa caaaccttag ggcagcacaa ttaaagccag aaacaactat
     1861 gcaagaaagt tactaaataa gtgctcggaa cagtaaaaca ctcagggggg actcagtccg
     1921 gattatgtaa tcagataaat tgccaaacat cacaaatcac cagccggact gagtgatgcc
     1981 gagcattgca ccaatacccc gtgatgaacg atgcctgatg cagaaagctc cctataaaac
     2041 aaagaacaaa aactacgacc ggcgattcac cgtcatactg atgctgcatc gtggtgacac
     2101 tgtctgtcac tttcatgttg actgaacaac tctgctgcga agaaggtgta ccgccggcgg
     2161 gatttgattg tccggcgtct agcttccact tccctgatgg aactgaaagt tgttatggcc
     2221 ggtcagcttg ccctgtccgc aggacatgag gtgctgtgac ggacagcacc agtaacggaa
     2281 agtgacaatg aaaatgaata ctgcgctgat agttgcacta atgtgtatgt ggtatgcggt
     2341 acccgctgct gcaaaagaga ctttactggc tatgccacgt aattcaacag aacattgcta
     2401 tgcggagatt aatgtgcacg gaccatatgg ggtgtatttc cgagtggtcc cgcatccccc
     2461 cgggggaaag agctgggtcg aatgtaacag tgactactat tactctgata aaccaccggg
     2521 agtacagata ctcggaaccc gtgcagggtg tcgggtatac ggaatctgcg ggaccacctc
     2581 gacactgcat gtggctggaa gaggggttgt gtgtattaaa aatatatgca gtccacgtgg
     2641 gatgataatt cacaggatac ggaaacgtcc tgtagtggct gtaagcgatg agatgtgatc
     2701 ttacccagca atagtagaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac
     2761 atgacaaaaa cagtatcaac cagcaaaaaa acacgtaaac agcattcgcc tgaattttgc
     2821 agtgaagccc tgaagcttgc tgaacgcatc ggtgttgctg ccgcagcccg tgaactcagc
     2881 ctgtatgaat ctcagctcta tgcgtggcgc agcaaactgc agcaacagat gacctcgtct
     2941 gagcgtgaaa gtgaactgcc cgcctgaagc ggcaactggc ggagcaggca gaggaactgg
     3001 ctatcctcca aaaggccgcg acatactttg cgcagcggct ggtatacgtg gtgtcagcgt
     3061 cggacaggaa taagccctcg tcagcagttc cgtcagcact gcgacagcgt tgtcctcgcg
     3121 gcggccttta cccggtcaaa acagcgttac ggtgcaccac gcctgacgga tgaactgcgt
     3181 gctcagggtt accactttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcaggggctg
     3241 agggcaaagg cctcccgg
//
LOCUS       DAJTUI010000210         3182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTUI010000210 DAJTUI010000000
VERSION     DAJTUI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.213"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            419..1528
                     /gene="fepE"
                     /locus_tag="OO464_004091"
     CDS             419..1528
                     /gene="fepE"
                     /locus_tag="OO464_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6229007.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2553..2747)
                     /locus_tag="OO464_004092"
                     /pseudo
     CDS             complement(2553..2747)
                     /locus_tag="OO464_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          802 a          659 c          776 g          945 t
ORIGIN      
        1 aaaaaaaaac attatcttat attaggggtg ctgctagcgg cgcggtgtgt ttttttacag
       61 gacgcccctg ggggcgctgc taggggtgtc tgttcagata tgtagcaatg agtcagggat
      121 tcacgaactg aaaatgtccg aaaatgtgtc tcatgggcac tgtatgcgcc caaaccgatc
      181 gtcattcact ttcatgcata gctatgcagt gagctgaaag cgatcctgac gcatttttcc
      241 ggtttacccc ggggaaaaca tctctttttg cggtgtttgc ggcagaatcg cgttcagcgc
      301 gttttagcgg tgcgcgcaat aaaatgttat gttaaatgtc tgctgaatgg tttttaggtt
      361 ttttcctaac atatgataga ataaaaaaac catatgttaa tatattgtgt ttttttcaat
      421 gaaaatctca aaggtctctg acgaacattt ttctaattac aacatgttgc cgaatggtaa
      481 tgaagaaatt gacctttttg gcttgattag cgttttatgg gcagcaaaaa taaaaattgt
      541 tggggtgatt ctattttttg cttgtattgg ctttcttgtt tcttttgttt tgccacaaaa
      601 atggactagt tctgctgtgg ttactcctgc tgagtctgtt caatggagtg aattaaataa
      661 ggagctttcc aagcttcatg ttctgggagt ggattttgat atcaatcgtg atagcgcatt
      721 tgctctgttt attaagaaat ttcagtccgt taattcgttg aatgagtata tgagatcatc
      781 tccttatgtt atggagatta ttaaaaagaa taacattagc gctctcgaac tgcatcgtgc
      841 aattgttgag ttgagtaaaa atatgaagtc gattgatgat aatgccagta agaaaaacga
      901 caagtcatca ttgtatgtat catggactct gagttttact gctccaacaa gtaaagaagc
      961 tcacgatgtg ttgtctgggt atattaatta tgtttcttcc cttgttgtaa gggatttgat
     1021 ggaagatata agaaataaac tagaagttaa aactaatgtt gaaaaagaaa ttcttgcact
     1081 ggatgagata aaaattagaa accagctgaa tgcagatatt cgacgcctca attattcact
     1141 ggaggttgct aatgcggctg gaataaaaaa acctgtatac agcaatggtc agattatgaa
     1201 ggatgaccca gattttcctg tggctctcgg ttctgatggt atagcaacta aattgaacat
     1261 caaaaaatca atcaaggatg tttcggaatt gagtggggag ttgcgaaatc gtcaatatgt
     1321 tgtgaatcaa ttggttgtgg cgaaagtggg ggatgttgat tttatgccat ttcagtatca
     1381 attgagccca acgttaccgg tcagaaagga tgggccaagt aatgccataa ttgtaattct
     1441 ttcttctgtc attggaggga tgcttgcatg tggctatata cttttacgtc atgccatgga
     1501 ggccagaaag atgagtgtga ttgagtaaaa gttgtgctga tgcatcattt tataagcaac
     1561 ttttataaaa agttcaaagg atcttcttga gatcattccc ttacagccgt agtcttctac
     1621 tctagaaaac gaaaaaacca cctggggagg tggtttttcg aaggttcagt aagttgggga
     1681 acttctgaac cgtggtaaca gtcttgtgcg agacatgtca ccaaatctgt ccttctagtg
     1741 tagcctcagt aaggctgcca cttcaagaac tctcaataca tctctcgcac atcctgttta
     1801 ccagtggccg ctgccaatgg cgttaagtcg tgtcttaccg ggttggactc aagacgatag
     1861 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1921 gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacgaac gcggccataa
     1981 cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccacc
     2041 agggggaaac gcctggtatc tttaagtcct gtcgggtttc gccacctctg acttgagcgt
     2101 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccac cggcgcggcc
     2161 ttttttcggg gcctcttttt tgctcacatg ttttttctgg tgttatcccc tgattctgtg
     2221 gataaccgta tatccgtctt tgcgtgagca gacaccgctc gccgcagccg aacgaccgag
     2281 cgcagcgagt cagtgagcga ggaagcggaa gagcgccgga agctgcattt tctccttacg
     2341 tatctgtgca gcatttcaca cctcatatgt cgctgttctc tcaccatctg ctctgccgcc
     2401 tcataattaa gccagtatac actccgttat cgctacgtga ctgggtcatg gctgcgcccc
     2461 gacacccgct aaaaccccct gacgcgccct gacgggcttg tctgctgacg aaaacgccca
     2521 cgggaaagcc gtgaccgcct ccgtcagctt cgttatgttc ctgtttcact ttcggcctca
     2581 aagcgaatct ggatgctgtt ctggagttct tctgcgagtt cgtgcagtcg tttgcacatg
     2641 gccgcctgtt cgtcggcatc cagagcatcc agtttttcaa gcagtgtcag gctctggctt
     2701 ttgatacatc gcgccatatt cagcgcagtt tgttgttatt tgttcacctt ccgttgttct
     2761 ccgtcagttc cgggtgtcgg ggggggggga ccctgagcag gtggtaattg taagacgttg
     2821 cgggcgacgg tattacaatt gcacatcctg tcctgtttct ttaggacgta tcatggcaca
     2881 gcatatcaac tgataaataa tccctgtgca gtgcagccgc tgttgcataa aatttcgctc
     2941 tgcgtcgagt gtttcttaat caataattcc gctgatactc cggaaaaacg cgagtctgtt
     3001 ctcatgcctg aaatgcccac accccacgca aaaacaagtt tttgctgatt tttctttata
     3061 aatagagagt tatgacaaat tagttcttct tgctctcttt gtgatattta aaaaagcggt
     3121 gtcggcgcgg tgttgtagct gcgccaacac cgctttttag gggtggtact gactattttc
     3181 at
//
LOCUS       DAJTUI010000211         3121 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTUI010000211 DAJTUI010000000
VERSION     DAJTUI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.214"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            42..1073
                     /gene="ynaI"
                     /locus_tag="OO464_004093"
     CDS             42..1073
                     /gene="ynaI"
                     /locus_tag="OO464_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6229008.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(1124..2737)
                     /gene="mppA"
                     /locus_tag="OO464_004094"
     CDS             complement(1124..2737)
                     /gene="mppA"
                     /locus_tag="OO464_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6229009.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT          829 a          716 c          683 g          893 t
ORIGIN      
        1 tccggcatga cgacaattat ctggaggagc ctgataaaac aatgatcgct gaactgttta
       61 caaataatgc gcttaatctg gtcattattt tcggtagctg cgcagcattg attctgatga
      121 gcttttggtt tcgccgtgga aatcgtaaaa gaaaaggatt tttattccat gcggtgcaat
      181 ttttaatcta caccataatt atcagtgctg ttggtagcat cagtaattat gttatagaaa
      241 actataaact caaatttatc accccaggcg ttatcgattt tatctgtacg tccctgattg
      301 cggttattct gacgattaag ctatttctgc tgattaatca gtttgaaaaa cagcagatta
      361 aaaaaggtcg cgatatcacc agtgcgcgga ttatgtcgcg cattatcaaa atcaccatta
      421 ttgtagtgct tgttctgctt tatggcgaac atttcggcat gagcctttct ggcttgctga
      481 cctttggtgg tattggtggt ctggctgtcg gtatggccgg taaagatatt ctgagtaatt
      541 tcttttccgg gatcatgctc tatttcgacc gtcctttcag tattggcgac tggatccgtt
      601 caccggacag aaatatcgaa ggtacagtag cggaaattgg ctggcgaatt accaaaatta
      661 cgacctttga taatcgtcca ttgtacgtac cgaactcgct gttttcgtcg atcagcgtag
      721 aaaacccagg acgaatgacc aaccgccgca ttaccacgac cattggttta cgttatgagg
      781 atgcggcaaa agtgggcgtt attgtcgaag ccgtacgtga gatgctgaaa aatcacccgg
      841 ccatcgacca gcgacaaacc ttactggttt atttcaacca gtttgctgac tcgtcattga
      901 atattatggt ttattgcttt accaaaacca cggtatgggc tgagtggctt gctgcacagc
      961 aagacgttta tttgaagatt atcgatattg tacagtcaca cggcgcggac tttgccttcc
     1021 ccagccagac gctgtatatg gataacatta caccgccgga acaggggcgc taaatcagac
     1081 atcactggcg ttatccacaa gggtaacgcc agttcccatc acatcaatgc ttcacaatat
     1141 acatagtccg actgtacgct acatcttcag ggttattaat gggataacct ttcagccacg
     1201 gtttgattaa tcgtccattg gtatattgat aaattggtgc aatcggtgct tgctccatga
     1261 ggattttttc tgccgcgttg taatcggcat tacgcgcttt aacggtattt tccgtcgatg
     1321 cctgggccag aactttgtcg tatgccggat tgttaaagcg tgaaatattt cctgaatgca
     1381 ttgacgttaa taatgtcagg aaagtggagg gttcattata atcccccacc catgaggcgc
     1441 ggataacatc aaaattgccg gtgttacggc tatcgatata ggttttccat tcctgatttt
     1501 gcaatttaac atctacgcca aggttctttt tccacatcga tgctacagca atcgcaattt
     1561 tttgatggtt ttctgaagtg ttatacaaaa gcgtcagctt cagcggtttt tgcggaccat
     1621 aaccagctgc gctcaacaaa gtttttgcct gcgcattcag ttcttcctga ctcatttgtt
     1681 caaacggcga aggttccggc gtaaatccgg cggtaacatc tggtataaaa tgccacgctg
     1741 gcttttcgcc cgtccccaac actttttcgg tcatcaggcg gcgatctatc gtcatactta
     1801 atgccaggcg aacgcgctga tctgccgtcg gccctttttg cgtgttaaag gcataataat
     1861 aggtcccgag ctgcggtggc gtataaacct gccccggaat atccttcaac agcttctgat
     1921 acatattttt cgggaaggat tcggtgatat caatatcccc cgcgaggtaa cgcttagtgg
     1981 ctgcggattc ctgattaatt ggcaggaagg tcactttttg cagcaccgtt ttggcgttat
     2041 cccaataatg ggtattcggt accacgacca gtttttcatt gactacgcgc tctttaagaa
     2101 cataagcgcc attgccgatc agatttccgg gtttcgtcca ctctttaccg ctttctacgt
     2161 tggctttttg caccgggaag aaggcaaagt tagcggttaa attcacaaac cacggcaacg
     2221 gtttatcaag ctgaattttc aaagtatggg catcaactgc ggtgacgcca agctgatcag
     2281 gcgtagcttt accatcaata atcgcctgtg cgttgttgat tcccgccagc gcggcaaacc
     2341 atgcaaatgg cgacaatgtt tttgggtcca ccagacgttg ccagctgtag acaaaatctt
     2401 gcgccgttac cggtgtgcca tccgcccatt ttgcgttatc gcgcagggta aaagtccaga
     2461 tacggttgtc attacttttc cattgagtcg cgacgccggg gacaatctcc cctttttcgt
     2521 tctgattcac cagaccttca aacagatcgc gaatgacctg aatctctggc aggcccacgg
     2581 ctttagcggg atccagcgac gcaggctcat ctttaatgtg gcgcaccagc tcctgcttct
     2641 ctgccagtac tgtgccgctc ggaacttctg cagcatacga aagagaaatg ctgctgacca
     2701 acagcgcaca acacgtaact gaaacagagt gcttcataag tttccctcta accgacatta
     2761 aaattgatgc gtaattattt gtttcgcctt aaagaaatgc aaacaacttc catcaaaaaa
     2821 gtgacaaagg aagcatcaaa tgttttaagt atcaatatat caaatcagat ttagtgtaaa
     2881 taatagacgc caaaatgtgg caaagcacat agaacaccga acactacgtt cgggcttcgc
     2941 acgttcaaac cagttttcgg tatcataatc agcatctgtc gccattgtac gaagaataaa
     3001 aaataaccaa aacgttgaaa ggaactgaga atacgttaag agaagaatta aattaatgta
     3061 tcagcaagta gtgctaaaca gcacctttat acttcagcct atctataaat aatgagaatg
     3121 c
//
LOCUS       DAJTUI010000212         3112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTUI010000212 DAJTUI010000000
VERSION     DAJTUI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.215"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO464_004095"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO464_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR6229010.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="OO464_004096"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="OO464_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO464_004097"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO464_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR6229011.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO464_004098"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO464_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR6229012.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="OO464_004099"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="OO464_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          753 c          757 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DAJTUI010000213         3071 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTUI010000213 DAJTUI010000000
VERSION     DAJTUI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.216"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..238
                     /locus_tag="OO464_004100"
     CDS             <1..238
                     /locus_tag="OO464_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR6229013.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="OO464_004101"
     CDS             248..886
                     /locus_tag="OO464_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR6229014.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="OO464_004102"
     CDS             889..2052
                     /locus_tag="OO464_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6229015.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..>3071
                     /locus_tag="OO464_004103"
     CDS             2136..>3071
                     /locus_tag="OO464_004103"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6229016.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRR"
BASE COUNT          692 a          725 c          880 g          774 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacattttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaact gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccagta acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg t
//
LOCUS       DAJTUI010000214         3067 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTUI010000214 DAJTUI010000000
VERSION     DAJTUI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.217"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            2..2905
                     /locus_tag="OO464_004104"
     rRNA            2..2905
                     /locus_tag="OO464_004104"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3001..>3067
                     /gene="rrf"
                     /locus_tag="OO464_004105"
     rRNA            3001..>3067
                     /gene="rrf"
                     /locus_tag="OO464_004105"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          812 a          676 c          957 g          622 t
ORIGIN      
        1 aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga tgaaggacgt
       61 gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc gatttccgaa
      121 tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg ttaatgaggc
      181 gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc
      241 cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga
      301 agcgtctgga aaggcgtgcg atacagggtg acagccccgt acacaaaaat gcacatactg
      361 tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg ggaccatcct
      421 ccaaggctaa atactcctga ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa
      481 agaaccccgg cgaggggagt gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag
      541 cacgcttagg cgtgtgactg cgtacctttt gtataatggg tcagcgactt atattctgta
      601 gcaaggttaa ccgaataggg gagccgaagg gaaaccgagt cttaactggg cgttaagttg
      661 cagggtatag acccgaaacc cggtgatcta gccatgggca ggttgaaggt tgggtaacac
      721 taactggagg accgaaccga ctaatgttga aaaattagcg gatgacttgt ggctgggggt
      781 gaaaggccaa tcaaaccggg agatagctgg ttctccccga aagctattta ggtagcgcct
      841 cgtgaattca tctccggggg tagagcactg tttcggcaag ggggtcatcc cgacttacca
      901 acccgatgca aactgcgaat accggagaat gttatcacgg gagacacacg gcgggtgcta
      961 acgtccgtcg tgaagaggga aacaacccag accgccagct aaggtcccaa agtcatggtt
     1021 aagtgggaaa cgatgtggga aggcccagac agccaggatg ttggcttaga agcagccatc
     1081 atttaaagaa agcgtaatag ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg
     1141 ctaaaccatg caccgaagct gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt
     1201 ctgtaagcct gtgaaggtgt gctgtgaggt atgctggagg tatcagaagt gcgaatgctg
     1261 acataagtaa cgataaagcg ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc
     1321 caacgttaat cggggcaggg tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat
     1381 gggaaacagg ttaatattcc tgtacttggt gttactgcga aggggggacg gagaaggcta
     1441 tgttggccgg gcgacggttg tcccggttta agcgtgtagg ctggttttcc aggcaaatcc
     1501 ggaaaatcaa ggccgaggcg tgatgacgag gcactacggt gctgaagcaa caaatgccct
     1561 gcttccagga aaagcctcta agcatcaggt aacatcaaat cgtaccccaa accgacacag
     1621 gtggtcaggt agagaatacc aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa
     1681 tggtgccgta acttcgggag aaggcacgct gatacgtagg tgaagcgact tgctcgtgga
     1741 gctgaaatca gtcgaagata ccagctggct gcaactgttt attaaaaaca cagcactgtg
     1801 caaacacgaa agtggacgta tacggtgtga cgcctgcccg gtgccggaag gttaattgat
     1861 ggggttagcg caagcgaagc tcttgatcga agccccggta aacggcggcc gtaactataa
     1921 cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acctgcacga atggcgtaat
     1981 gatggccagg ctgtctccac ccgagactca gtgaaattga actcgctgtg aagatgcagt
     2041 gtacccgcgg caagacggaa agaccccgtg aacctttact atagcttgac actgaacatt
     2101 gagccttgat gtgtaggata ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag
     2161 ccgaccttga aataccaccc tttaatgttt gatgttctaa cgtggacccg tgatccgggt
     2221 tgcggacagt gtctggtggg tagtttgact ggggcggtct cctcctaaag agtaacggag
     2281 gagcacgaag gttggctaat cctggtcgga catcaggagg ttagtgcaat ggcataagcc
     2341 agcttgactg cgagcgtgac ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg
     2401 gttctgaatg gaagggccat cgctcaacgg ataaaaggta ctccggggat aacaggctga
     2461 taccgcccaa gagttcatat cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat
     2521 cctggggctg aagtaggtcc caagggtatg gctgttcgcc atttaaagtg gtacgcgagc
     2581 tgggtttaga acgtcgtgag acagttcggt ccctatctgc cgtgggcgct ggagaactga
     2641 ggggggctgc tcctagtacg agaggaccgg agtggacgca tcactggtgt tcgggttgtc
     2701 atgccaatgg cactgcccgg tagctaaatg cggaagagat aagtgctgaa agcatctaag
     2761 cacgaaactt gccccgagat gagttctccc tgacccttta agggtcctga aggaacgttg
     2821 aagacgacga cgttgatagg ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta
     2881 ctaatgaacc gtgaggctta accttacaac gccgaagatg ttttggcgga ttgagagaag
     2941 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaattag
     3001 cctggcggcc gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
     3061 cgtagcg
//
LOCUS       DAJTUI010000215         3039 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTUI010000215 DAJTUI010000000
VERSION     DAJTUI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.218"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..546
                     /locus_tag="OO464_004106"
     CDS             <1..546
                     /locus_tag="OO464_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6229017.1"
                     /translation="YQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLADVLKTALL"
     gene            958..1326
                     /locus_tag="OO464_004107"
     CDS             958..1326
                     /locus_tag="OO464_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229018.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            1757..2272
                     /locus_tag="OO464_004108"
     CDS             1757..2272
                     /locus_tag="OO464_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229019.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            2262..2762
                     /locus_tag="OO464_004109"
     CDS             2262..2762
                     /locus_tag="OO464_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229020.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
BASE COUNT          726 a          794 c          766 g          753 t
ORIGIN      
        1 tatcagttca cacgacacac ggctcacacg acctctgagg cccgtagatg ggcgtctctg
       61 gtgtctgtgg tcactggcgc tcggtctgct gaggtctgcc acctgactaa acgtgacatt
      121 gtaaccttgg acaatggact ggtctgtatc gacatcaatg aagatgggga cgggaagtct
      181 gtgaagaaca aacattcagt ccgacttgtg ccgttaaccg atggcgctta tgggtttgac
      241 ctgacgtcct tcctgtcgtg ggtagacatg cagcctgatg agggtcctct cttcgggatg
      301 actccgagcg cctactctag ctggtttaac tcaagggtcc tgactgaggc cctaggagac
      361 tctcaggatg tctctctcca cagtctgaga cattggctgg cgacccgaat gaaagagcgt
      421 ggagtcaatc tggtggacgc tcaaggcatc ttaggtcaca gcagtcagtc aattacgtat
      481 gacctgtacg ggaaaggaca cgctgtgggt aggctggctg atgtgcttaa gacagcttta
      541 ctttaagtag ggagacccaa ggtggagacc atatacggct tgatggttga gaccttgggt
      601 cactgaagtc tgactgaagt tagggagggt gataataatc ataatccctc ctgactatag
      661 gcccactggt ccgccttcgg ctgtccgact ctaagtctgt ctgtggtctt atttaaactg
      721 cgtcctaaac tccagtccct ccaaattcct atggtctcct cttgacattc ctgtttaact
      781 gtgagtcgcc tctggtctct ctttatatat acttccttaa ttcctgaggc gtctttagtc
      841 cttgacattc ggttcaacct gtgagtcaca tcctgtcgcg ctactgtatg catcaccagt
      901 gatggacttg attatcacta taggggaacc aactaattca actacaggag atactctatg
      961 gcacgaccaa tcgcaactca cgacaacacg ttcaccaaag cctacttgca gcagcactgc
     1021 ggggacctgc tgtcattcga tggtcagggc gacctgagtg gttggctgga tgatgtcctt
     1081 actggtgctg gtcgcctgaa cgaatcaatg gcaagcaata ccaagcctgt cagcccatac
     1141 ttgattctga cccaactact aacgcacgac acattaactg taagcgctgt gcaggagtcc
     1201 ctgagccgta agcgtgttgc cttgggtgaa cctatggttt caactcgcta cgctcgctac
     1261 gtctatgcta ccgtcgtcag cgccagtaaa tccgtccagt accacgccag taaagctggt
     1321 tcatgattac ggaccctaaa ctatcactat agggagacca gcctccgcct ctgagtctga
     1381 tgcgtcagaa catgcgagca tctggtcaac cgacattaag tatctctcct aagtaatcaa
     1441 ggcccgtgac cgcctctgac agacctcctg tatcgacctc cagctcgtta gcgttcttta
     1501 gtcgtctcgg ttagtagctc gtctactcac tacattggct taagtctatc accgctcacc
     1561 atctggagtc cttacagctc gtcctgacat gcggtcgcac ctttatacgt acatggagaa
     1621 catggtcact ccagaccgtc aacatttaac cattcacttt cgttaagcac tttctgatgg
     1681 gtccgtttag gatgcgtctg aatggtgcgg actccatgtg gagacagaaa gtgctttacc
     1741 aaaggagcat ctttgtatga ataccgaatt accagtaact aaccgccagt tcttaatcat
     1801 cctgctgacc aatgcagagt ccagtgtcga ttgctgtcgc tataacttag acctgctgaa
     1861 gttatcccga tggtatgccc aggcggaaga ctgggactat gagaaaatcc gggagatgta
     1921 cgcggagcct atccagtcat acctccagac acacaccgag gaagagctga agcgtggcct
     1981 gcaggacatc ttctatttca acaatcctta cacctgtgag ggtgactcag aagagatgga
     2041 ttggatagcg tcacgctctg atgccgactt gaagcacatc tctaacgcga tgatgcaggt
     2101 ggagagcaaa tggtcccgcc gccttcaggt cctaactgaa gattacgaaa acggcacctt
     2161 tgataagctg gtcccacctg atgccgaacg ccttcaggcg aaagctgacc aagaacagcg
     2221 cttcaagtcc tttgccgaag ctctggcggg aggtgcctct tatgacttct gacaataagc
     2281 aggaatacac cattcatatc ttcggtcagg acatcaccag tcaggacgga ttgtttcgac
     2341 tggatgagat acgccgggta ggcattcaga ttggtaaggt agaagataac cgtagtaccg
     2401 aggttgctcg cttcatgagg accaaggctg gtagaagtct aaccaaccca gaaaacgggt
     2461 ttgttaaaaa gattaacatg ggtcacttag ggacctcgtg gctggctgac ccagtagccg
     2521 ctatagagtt cggtcggtgg ttggaccttg ggtacggact ggcagtaacc cgagccttcg
     2581 tgtctatgac ctccagcgcc accgtgcagt ctgctgtagc gtctgctgag gacatcaagc
     2641 cggagaccaa ggaattcatc cagcgtcatg gcacaggcct ctacatcgcc ggacgtgaag
     2701 tctcacaggc tgagtattca gactatcaag ctgtgaccgc tggtcgctgt gagtggaggt
     2761 aagtaatcat ggctatgatt gaccctcgta cacctatcgg caaagcgacc cttcgttacc
     2821 gtggcctgcc cacacgtcat ctgctgtctc tgctgagact tggagtcgaa gaccctgagc
     2881 gcccttacta cagtcgagac gagctgatag ctatgctggt ggaccgtgat ttagacaatc
     2941 agttacgccg agcctttgct aagcagtcct gaccatacca aacctaattt taaacctttt
     3001 gagatgaccg atgcggggag acccgtaggc gctactgcg
//
LOCUS       DAJTUI010000216         3005 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTUI010000216 DAJTUI010000000
VERSION     DAJTUI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.219"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..197
                     /locus_tag="OO464_004110"
     CDS             <1..197
                     /locus_tag="OO464_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6229021.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="OO464_004111"
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="OO464_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR6229022.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="OO464_004112"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="OO464_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR6229023.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..2576
                     /locus_tag="OO464_004113"
     CDS             1821..2576
                     /locus_tag="OO464_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR6229024.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          786 a          671 c          709 g          839 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcag gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccggcttc atttttatgg ctgcacagta actttatttg tttaattaat acctaaccag
     2581 atgaaaaatt tgctcaaaat ttttaaatta acttattcaa cagtcacgca ttatattaaa
     2641 taacatttga ctgggttgaa cataacgcca atagcaaaag gagttatgtt caacaattta
     2701 attttagata tacatataac ttgttctatt aataaatcac ctcgtattct gactcggccc
     2761 ctggcgcagc aaagtcaggc atttatactc tttttaacac atagaaaatt catatcaata
     2821 taataaaaac ataaaatatt tgcatataca tataatctta aagtttagtc tatttaatgt
     2881 tcaatgaaat atttctacct gtataatctt taaagatgtt gaacatatat tcacattaaa
     2941 tatgattatg tacttgttac aaggataagg ttataaatga ataaagttac aaaaacagct
     3001 attgt
//
LOCUS       DAJTUI010000217         2951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTUI010000217 DAJTUI010000000
VERSION     DAJTUI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.221"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="OO464_004114"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="OO464_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR6229025.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="OO464_004115"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="OO464_004115"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6229026.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="OO464_004116"
     CDS             2603..>2951
                     /locus_tag="OO464_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6229027.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          701 c          779 g          813 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAJTUI010000218         2942 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTUI010000218 DAJTUI010000000
VERSION     DAJTUI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.222"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..443)
                     /gene="tetR(B)"
                     /locus_tag="OO464_004117"
     CDS             complement(<1..443)
                     /gene="tetR(B)"
                     /locus_tag="OO464_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6229028.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLE"
     gene            525..1730
                     /gene="tet(B)"
                     /locus_tag="OO464_004118"
     CDS             525..1730
                     /gene="tet(B)"
                     /locus_tag="OO464_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6229029.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1843..2436)
                     /gene="tetC"
                     /locus_tag="OO464_004119"
     CDS             complement(1843..2436)
                     /gene="tetC"
                     /locus_tag="OO464_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6229030.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2569..2940
                     /locus_tag="OO464_004120"
     CDS             2569..2940
                     /locus_tag="OO464_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6229031.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
BASE COUNT          804 a          534 c          600 g         1004 t
ORIGIN      
        1 tcttccaata cgcaacctaa agtaaaatgc cccacagcgc tgagtgcata taatgcattc
       61 tctagtgaaa aaccttgttg gcataaaaag gctaattgat tttcgagagt ttcatactgt
      121 ttttctgtag gccgtgtacc taaatgtact tttgctccat cgcgatgact tagtaaagca
      181 catctaaaac ttttagcgtt attacgtaaa aaatcttgcc agctttcccc ttctaaaggg
      241 caaaagtgag tatggtgcct atctaacatc tcaatggcta aggcgtcgag caaagcccgc
      301 ttatttttta catgccaata caatgtaggc tgctctacac ctagcttctg ggcgagttta
      361 cgggttgtta aaccttcgat tccgacctca ttaagcagct ctaatgcgct gttaatcact
      421 ttacttttat ctaatctaga catcattaat tcctaatttt tgttgacact ctatcattga
      481 tagagttatt ttaccactcc ctatcagtga tagagaaaag tgaaatgaat agttcgacaa
      541 agatcgcatt ggtaattacg ttactcgatg ccatggggat tggccttatc atgccagtct
      601 tgccaacgtt attacgtgaa tttattgctt cggaagatat cgctaaccac tttggcgtat
      661 tgcttgcact ttatgcgtta atgcaggtta tctttgctcc ttggcttgga aaaatgtctg
      721 accgatttgg tcggcgccca gtgctgttgt tgtcattaat aggcgcatcg ctggattact
      781 tattgctggc tttttcaagt gcgctttgga tgctgtattt aggccgtttg ctttcaggga
      841 tcacaggagc tactggggct gtcgcggcat cggtcattgc cgataccacc tcagcttctc
      901 aacgcgtgaa gtggttcggt tggttagggg caagttttgg gcttggttta atagcggggc
      961 ctattattgg tggttttgca ggagagattt caccgcatag tccctttttt atcgctgcgt
     1021 tgctaaatat tgtcgctttc cttgtggtta tgttttggtt ccgtgaaacc aaaaatacac
     1081 gtgataatac agataccgaa gtaggggttg agacgcaatc gaattcggta tacatcactt
     1141 tatttaaaac gatgcccatt ttgttgatta tttatttttc agcgcaattg ataggccaaa
     1201 ttcccgcaac ggtgtgggtg ctatttaccg aaaatcgttt tggatggaat agcatgatgg
     1261 ttggcttttc attagcgggt cttggtcttt tacactcagt attccaagcc tttgtggcag
     1321 gaagaatagc cactaaatgg ggcgaaaaaa cggcagtact gctcggattt attgcagata
     1381 gtagtgcatt tgccttttta gcgtttatat ctgaaggttg gttagttttc cctgttttaa
     1441 ttttattggc tggtggtggg atcgctttac ctgcattaca gggagtgatg tctatccaaa
     1501 caaagagtca tcagcaaggt gctttacagg gattattggt gagccttacc aatgcaaccg
     1561 gtgttattgg cccattactg tttgctgtta tttataatca ttcactacca atttgggatg
     1621 gctggatttg gattattggt ttagcgtttt actgtattat tatcctgcta tcgatgacct
     1681 tcatgttaac ccctcaagct caggggagta aacaggagac aagtgcttag ttatttcgtc
     1741 accaaatgat gttattccgc gaaatataat gaccctcttg ataacccaag agggcatttt
     1801 ttacgataaa gaagatttag cttcaaataa aacctatcta ttttatttat ctttcaagct
     1861 caataaaaag ccgcggtaaa tagcaataaa ttggcctttt ttatcggcaa gctcttttag
     1921 gtttttcgca tgtattgcga tatgcataaa ccagccattg agtaagtttt taagcacatc
     1981 atcatcataa gctttaagtt ggttctcttg gatcaatttg ctgacaatgg cgtttacctt
     2041 accagtaatg tattcaaggc taattttttc aagttcattc caaccaatga taggcatcac
     2101 ttcttggata gggataaggt ttttattatt atcaataata taatcaagat aatgttcaaa
     2161 tatactttct aaggcagacc aaccatttgt taaatcagtt tttgttgtga tgtaggcatc
     2221 aatcataatt aattgctgct tataacaggc actgagtaat tgttttttat ttttaaagtg
     2281 atgataaaag gcacctttgg tcaccaacgc ttttcccgag atctcatcta ttgaaacagc
     2341 ttgatagcct ttttcaacaa acaatattcg tgctgagtta accagtgatt gataggtact
     2401 cttaaaattt tcttgttgat gatttttatt ttccatgata gatttaaaat aacataccgt
     2461 cagtatgttt atggtatcat gatgatgtgg tcgtgacaat cttaagaaca tttaggttat
     2521 tttatgtata ttgaacagca ttctcgctat caaaataaag ctaataacat ccaattaaga
     2581 tatgatgata agcagtttca tacaacggtt atcaaagatg ttctattatg gattgaacat
     2641 aatttagatc agtctttact gcttgatgat gtggcgaata aagcgggtta taccaagtgg
     2701 tattttcagc ggctgttcaa aaaagtaaca ggggtcacac tggctagcta tattcgtgct
     2761 cgtcgtttga cgaaagcggc tgttgagttg aggttgacga aaaaaactat ccttgagatc
     2821 gcattaaaat atcaatttga ttcccaacaa tcttttacac gtcgatttaa gtacattttt
     2881 aaggttacac caagttatta tcggcgtaat aaattatggg aattggaggc aatgcactga
     2941 ga
//
LOCUS       DAJTUI010000219         2878 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTUI010000219 DAJTUI010000000
VERSION     DAJTUI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.223"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..779
                     /gene="lsrF"
                     /locus_tag="OO464_004121"
     CDS             <1..779
                     /gene="lsrF"
                     /locus_tag="OO464_004121"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR6229032.1"
                     /translation="WGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAP
                     LFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNS
                     CAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRI
                     AAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVD
                     MGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="OO464_004122"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="OO464_004122"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR6229033.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1133..1891
                     /gene="tam"
                     /locus_tag="OO464_004123"
     CDS             1133..1891
                     /gene="tam"
                     /locus_tag="OO464_004123"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR6229034.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1895..2809)
                     /locus_tag="OO464_004124"
     CDS             complement(1895..2809)
                     /locus_tag="OO464_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR6229035.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEEPEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          754 a          664 c          772 g          688 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactat cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aaatgtctga
     1141 ctggaacccc tctttatatc tgcacttttc cgctgaacga tcgcgtccgg cggtggagct
     1201 gcttgccaga gtgccgctgg agaatgtcga atatgttgcc gatcttggct gtggcccagg
     1261 taacagcacc gccctgctgc atcaacgttg gcctgcggcc aggataacag gcatcgactc
     1321 gtctccggcg atgattgctg aagcgcgcag tgctttgcca gactgccagt tcgtggaagc
     1381 agacatccgc aactggcaac cggaacaggc actcgatctg atttttgcta atgcctcact
     1441 gcaatggctg cccgaccact acgaattgtt tcctcacttg gtttctttac ttaatccgca
     1501 gggcgtgctg gcagtacaga tgccagataa ctggctggag ccgacccatg tgctcatgcg
     1561 cgaagttgct tgggaacaaa actacccgga tcgcgggcgt gagccgttgg ctggcgttca
     1621 tgcttactac gatattttga gcgaagccgg atgtgaggtc gatatctggc gaaccactta
     1681 ctatcaccag atgccgtcgc accaggcgat tatcgattgg gtgaccgcca ctggattacg
     1741 tccgtggtta caggatctga ccgagagcga acagcagctt tttcttacgc gctaccatca
     1801 gatgctggaa gagcagtatc cactgcaaga gaacggacag atactgctgg catttccgcg
     1861 tctgtttatt gttgcccgcc gtacggagta attatcacgt cagctggtaa tgacgatcgg
     1921 gaagaatttt tgctggaatt tctgcttcat cgttcatctg taacaggtca atttcaatag
     1981 cgttgcagat ggcatccagc ggtaaatcat tgttttcagt accgaacgga tcttccggtt
     2041 cttccgccag acaatccagc gaaataaaag tgtaggaaat cagcacagag ataaaaggcg
     2101 tcatgtaatg caggtccacg accagcgcga acggcagcat gatacaaaac agataaacgg
     2161 tacgatgcaa aatcagggtg taggcaaagg gaattggcgt gtaggcaatg cgctcacagc
     2221 ctgccagaac cgctgaaata tcattaagcc gatcgttgag gctaataaac aggatatctg
     2281 aaagctgtcc attgcggcgc tgaaccgcca accattctcc cattattaac aagatacggt
     2341 tagctggaga gttcgaagcc agtacacgct gaagatctcc agtcttgaga taatgagcca
     2401 gcacttccgc ctgtggctgt ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt
     2461 gcagcctggc aaactccctt acacttgccg aatccggcaa tgtcgttttt acctctcgca
     2521 gtaacgaccg tgaggcaatc atcaactgcc cccaaagttt tcgcgcttca acgtaacggg
     2581 cgtacccggc attattacga aaaccaagaa aaatggcgat ggcgacaccg agaatgctga
     2641 acggtgcgag ggtgaatttg atgcccagat gcgtgtacca gggcagcatg aatataacag
     2701 caatagaaaa gagaaaattg agtagtaagc gcgaggatat cttggataat actgagccgt
     2761 gccagacaaa aatacggcgc agccagtgtt gttgtggacg aacaatcatg gttatcttca
     2821 ggcgtggaaa agtcgcccta ttaaacgtga ttacgatcac attctcaaga cgctactt
//
LOCUS       DAJTUI010000220         2878 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTUI010000220 DAJTUI010000000
VERSION     DAJTUI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.224"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..770
                     /locus_tag="OO464_004125"
     CDS             <1..770
                     /locus_tag="OO464_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6229036.1"
                     /translation="VPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKVP
                     QPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRRVA
                     WINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKMR
                     YDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRYA
                     HLAPNHLTEHARQIDSILNPSVPNSSQSKNKEGTNDV"
     gene            1119..2291
                     /locus_tag="OO464_004126"
     CDS             1119..2291
                     /locus_tag="OO464_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6229037.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKMHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2345..>2878)
                     /locus_tag="OO464_004127"
     CDS             complement(2345..>2878)
                     /locus_tag="OO464_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6229038.1"
                     /translation="INGGNFHRVSSARYTHTNIQVTSSGGGKVTVILNGTTFQSAGDY
                     QPSAEQPYWITGANCEVVDIGCVFTETTSKVTSASALSVTRSGKINANGSIDVATGVS
                     SVNVVSTGVYDVSFSHPLAAVASGYIVQITPISAPDSVSCDVTYIGVDTFRVTLRNTL
                     SGAGISSSFAFSITRLI"
BASE COUNT          898 a          589 c          570 g          821 t
ORIGIN      
        1 ctgttccaga atacacgcca aaaccagcgt ccgttgcaac gaaggctacg catctttcat
       61 ttataaaggc cctactaaga gccgcagagc gtgaatggaa aatgctggat aaggcaccaa
      121 ttattaaagt gcctcaacca aagaataaac ggatccgctg gctggagccc catgaagcac
      181 aaaggctgat tgatgaatgt ccggagccat taaagtctgt tgttgaattt gcactggcaa
      241 caggcttaag acgctcgaac atcatcaacc ttgaatggca acaaatagat atgcagcgcc
      301 gggtggcatg gataaacccg gaagagagta aatcaaaccg cgcaattggc gttgcgctga
      361 atgatactgc atgtcgcgta ttgaaaaaac aaatcgggaa tcatcaccgt tgggtatttg
      421 tgtacaagga aagctgtacc aaaccagacg gaacgaaagc gccaacggtc aggaagatgc
      481 ggtatgacgc aaacacagcc tggaaagcgg cgctgagacg agcaggtatt gatgatttca
      541 gatttcacga cttgagacac acctgggcaa gttggctggt tcaagccgga gtcccgttgt
      601 cagtgttaca ggaaatggga ggctgggagt ctatcgaaat ggttcgtcga tatgctcacc
      661 ttgcacctaa tcaccttacc gaacacgcac ggcaaataga ctcgatcctg aacccatcgg
      721 tcccaaattc gtcccagtca aaaaataagg aaggtactaa tgatgtgtaa cttattgatt
      781 taaatggtgc cgataatagg agtcgaacct acgaccttcg cattatgaat ctgtagcacc
      841 aatcataact atctgtttta gcaagcatta accgcattca ctaagctata gttgatgaca
      901 caaacagaaa gttgatgcat gatgttgcca tatatatgtc acaaatacgg cacaacgatc
      961 ttcaaacata tagccacaca ttcacagaag agcacaaagc cttgcaatcc agtgcaaagc
     1021 ttggtgtgtc tccgttttgt ttcatcagtt acacataact atatgatcat taactaaaag
     1081 tcagtatact tggagaaatt ttaattgcgt aatagagcat gcatatgatt gaaattaaca
     1141 gtttattatt aataacatcc gtgatattga tgtcgttatt agctgtgggg ttatttgata
     1201 aaatttcccc aataaatctt gttgagcacg gcaggaataa tcaaatagat ggaatgcggg
     1261 ggttcttagc aattttcgtg cttattcatc acgcagcaat ttggaatgga tacttgtcat
     1321 ctggagtatg ggaagcacct tcatcaaatc tgttagcaaa tttaggccaa gttggtgtgt
     1381 cattcttttt tatgattact ggttatctgt tcttttcaaa gattatctct ggagatcagg
     1441 actggacaag gctttatgta tcaagattac tacgattaac cccaatgttc atagttagcc
     1501 tatgtctcat ttttatcatt gtaggtttta agtctggatg gagaatgcag gtatccacag
     1561 aagagctttt tgtgtcaata atgaagtggt tgccattcac tgcgctaggt atgccgaaca
     1621 ttaatgacgt aaaagattca tttactatca atgccgctgt aacatggaca cttgtatatg
     1681 aatggttctt ttatttttct cttccggtaa tttccgcgct cataaaaaga aaagtcagta
     1741 tttacatggt tatgattagc gcaatatcgc tatttgtttt cattttattt ttcagtaaaa
     1801 tgcacatagc atcatttctt tttgggctcc tggcattttt gctaaataaa tcaaagatag
     1861 tcaatggaat tgccaaagca aaagtaacac caattataat aactgcgata atggtttttg
     1921 aaatgacgta tttcaaaaca acttacgcac cgctaccact gattctttgt ggaataacat
     1981 ttatcattat cgcatcaggt tgtgatttgt atggaatatt aagattaaat ataaccagaa
     2041 agctaggaga aactacctac agcgtttacc ttctgcatgg gatattcctt tattgcctaa
     2101 tgacgtggat tattcctaat aattacactg aaaatacatt tatcatactg gtaagcacaa
     2161 ctgcatttct tattacgttc acatcatgcc taacttttaa attaatagag acgccattca
     2221 tcaaattaac aaaacaaacc acaactttag taaaagaatt aatacccaca ttaacaaaca
     2281 acaatcaata aacaataaac ataagccgcc ctacatatat gtccaaatgg ggcggcgaat
     2341 gtatttatat tagtctggta atgctaaatg caaacgaact ggagattcct gccccgctaa
     2401 gcgtattgcg cagggtcacc ctaaatgtat ctacgccaat ataagtaaca tcacaactaa
     2461 ctgaatcagg cgcagatata ggtgttatct gcacaatata accgctagca actgctgcga
     2521 gcggatgaga aaagctaaca tcatacacac ctgtagaaac cacgttaacc gatgaaacac
     2581 cagtagcaac gtcgatacta ccattggcgt taatttttcc cgagcgtgtg actgacaatg
     2641 cagaagccga tgttacctta ctggttgttt ctgtaaaaac acacccaatg tcaactacct
     2701 cgcaattagc tcctgttatc cagtatggtt gttctgcact tggctgataa tcccctgcgc
     2761 tctggaacgt tgtgccgttg agtatgactg taacttttcc cccacctgag cttgttactt
     2821 gaatattcgt gtgcgtgtat cttgcactgc tcacgcgatg aaaatttcca ccgtttat
//
LOCUS       DAJTUI010000221         2852 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTUI010000221 DAJTUI010000000
VERSION     DAJTUI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.225"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            78..653
                     /gene="ssuE"
                     /locus_tag="OO464_004128"
     CDS             78..653
                     /gene="ssuE"
                     /locus_tag="OO464_004128"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR6229039.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            646..1605
                     /gene="ssuA"
                     /locus_tag="OO464_004129"
     CDS             646..1605
                     /gene="ssuA"
                     /locus_tag="OO464_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6229040.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1602..2747
                     /gene="ssuD"
                     /locus_tag="OO464_004130"
     CDS             1602..2747
                     /gene="ssuD"
                     /locus_tag="OO464_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR6229041.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2756..>2852
                     /locus_tag="OO464_004131"
     CDS             2756..>2852
                     /locus_tag="OO464_004131"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HCR6229042.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSV"
BASE COUNT          648 a          778 c          776 g          650 t
ORIGIN      
        1 tcttgtctct ccgacagact gaccgaattg ttatcaatgt taacaaaaaa aagaacaatt
       61 ggttataagg agagagtatg cgtgtcatca ccctggcggg tagtcctcgc tttccttctc
      121 gctccagctc cttgctggaa tatgcgcggg aaaaactaaa tggcctagat gtagaggttt
      181 atcactggaa tctgcaaaac ttcgccccgg aagatctgct ttatgctcgt ttcgatagtc
      241 cggcactcaa gaccttcacc gaacagctgc aacaggctga tgggctgatt gtcgccacgc
      301 ctgtgtataa agccgcctat tccggcgcgc tgaaaaccct gctcgacctg ctgccagagc
      361 gcgctttgca aggcaaagtg gtgctaccgc tggcgacggg cggtaccgtg gcccatctgc
      421 tggcggtcga ttatgccctt aaaccagttt taagcgcact gaaagctcag gagatcctgc
      481 acggcgtgtt tgccgatgac tcacaattaa ttgattacca tcacagaccc cagttcacgc
      541 caaatctgca aacccatctt gataccgcgc tagaaacttt ctggcaggca ttgcaccgcc
      601 gcgatgttca ggttcctgac cttctgtctc tgcgaggtaa tgcccatgcg taacatcatt
      661 aaactggcgc tggcgggatt gcttagcgtc tctacttttg cggttgctgc agaatcctcg
      721 cctgaagcgt tacgtatagg ctatcagaaa ggcagtattg gtatggtgct ggcaaaaagc
      781 caccagttac tggaaaaacg ctatccgcaa tcaaaaatct cctgggtgga gttccccgcg
      841 ggtccgcaaa tgttggaagc gttaaacgtt ggcagtattg atctcggcag taccggggat
      901 attccgccaa tctttgccca ggctgccggg gctgatttgg tatatgtggg cgtcgagcca
      961 ccgaagccca aagccgaagt gattctggtg gcagaaaaca gcccgatcaa aaccgtagcc
     1021 gatcttaaag gtcacaaagt tgcctttcag aaaggttcca gttcacacaa ccttttactg
     1081 cgtgcactgc gtcaggccgg acttaaattc accgatatcc agcccactta cctgacgcct
     1141 gccgatgccc gcgccgcgtt ccagcaaggt aacgttgacg cctgggctat ctgggatccc
     1201 tactactccg ctgcgttatt acagggcggc gtgcgggtgc tgaaagacgg caccgatctc
     1261 aatcaaactg gatcgtttta tctggcagct cgtccctatg cagaaaaaaa cggcgctttt
     1321 attcagggcg tactggcaac ctttagtgag gccgatgcgt taacccgcag ccagcgcgaa
     1381 caaagcatcg ctttactggc aaaaacgatg ggcttaccgg caccggtgat tgcctcttac
     1441 ttagatcatc gccctcctac caccatcaaa ccggttaacg ccgaggttgc cgccttacag
     1501 cagcaaacgg cagatctgtt ttatgaaaac cgtctggtgc cgaaaaaagt cgatattcgc
     1561 cagcgcatct ggcagcccac tcaactggaa ggaaaacaat tatgagtctg aatatgttct
     1621 ggtttttacc gacccacggt gacgggcact atctgggaac ggaagaaggt tcacgcccgg
     1681 ttgatcacgg ttatctgcaa caaattgcgc aagcggcgga tcgtctgggc tataccggtg
     1741 tgctgatccc aacggggcga tcgtgtgaag atgcgtggct ggttgcagcg tcgatgatcc
     1801 cggtgacgca gcggctgaag tttcttgtcg ccctgcgtcc cagcgtaacc tcacctactg
     1861 ttgccgcccg ccaggccgcc acgcttgacc gtctctcaaa tggtcgtgcg ttgtttaatc
     1921 tggtcacagg cagcgatcca caagagctgg caggcgacgg cgtgttcctt gatcatagcg
     1981 agcgctacga agcctcggcg gaatttaccc aggtctggcg gcgcttattg ctgggggaaa
     2041 ccgtcaattt caacggtaaa catattcatg tgcgcggagc aaaactgctc ttcccgccga
     2101 ttcaacagcc ttatcctccg ctttactttg gcggatcgtc agatgtcgcc caggagctgg
     2161 cggcagaaca agttgatctc tacctcacct ggggcgaacc gccggaactt gttaaagaga
     2221 aaatcgaaca agtgcgggcg aaagctgccg cgtatgggcg caaaattcgt ttcggtattc
     2281 gtctgcatgt gattgttcgt gaaactaacg acgaagcgtg gcaggccgcc gagcggttaa
     2341 tctcgcatct tgatgatgag actatcgcca aagcacaagc cgcattcgcc cggacggatt
     2401 ccgtaggaca acagcgaatg gcggcgctac ataacggcaa gcgcgacaat ctggagatca
     2461 gccccaattt atgggcgggc gttggcttag tgcgcggcgg tgccggaacg gcgctggtgg
     2521 gcgatggtcc tacggtcgcc gcgcgaatca acgaatacgc cgcgcttggc atcgacagct
     2581 ttgtgctttc gggctacccg catctggaag aagcgtatcg ggttggcgag ttgctgttcc
     2641 cacatctgga tgtcgccatt ccggaaattc cccagccgca gccgctgaat ccgcaaggcg
     2701 aagcggtggc aaatgatttt atcccccgta aagtcgcgca aagctaagga acacgatgat
     2761 ggcaacgcca gtgaagaagt ggttattgcg cgttgccccc tggtttttac cggtgggcat
     2821 cgtggcggtg tggcaactgg cctcctcggt tg
//
LOCUS       DAJTUI010000222         2807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTUI010000222 DAJTUI010000000
VERSION     DAJTUI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.226"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            6..81
                     /locus_tag="OO464_004132"
     tRNA            6..81
                     /locus_tag="OO464_004132"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="OO464_004133"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="OO464_004133"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="OO464_004134"
     CDS             complement(299..1057)
                     /locus_tag="OO464_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR6229043.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="OO464_004135"
     CDS             complement(1065..2168)
                     /locus_tag="OO464_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6229044.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="OO464_004136"
     CDS             complement(2178..>2807)
                     /locus_tag="OO464_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6229045.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          763 a          739 c          675 g          630 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaataacgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAJTUI010000223         2638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTUI010000223 DAJTUI010000000
VERSION     DAJTUI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.227"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            40..2562
                     /locus_tag="OO464_004137"
     CDS             40..2562
                     /locus_tag="OO464_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6229046.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
BASE COUNT          709 a          566 c          662 g          701 t
ORIGIN      
        1 aaaatgacgc tgtaagcaag ggataaggat aggcgtatta tgatgacgac cagaatagtg
       61 gttggcctca cggcagggac gtgtctgatt ttctcgcaaa acctgatggc cgaggtcagt
      121 gtattcaatc cggcgcttct ggaaatcaac catcaatccg gagtcgatat tcgccagttt
      181 aatcgggcaa acctgatgcc cccaggtgtt tatagcgttg atatttttat caacggtaaa
      241 atgtttgaac gtcaggatgt gacatttgtt caggataatc cagatgctga tctgcacgct
      301 tgctttattg ccattaaaaa aacactgtcc tcctttggca taaaagttga tgcgctcaaa
      361 tcgttcaatg atgtggatga gacggtttgc ctcgatcctg ctccacgtat tgaaggctca
      421 tcctggcagt ttgacagtga taaattgcag ctgaatatat ccattcacca aatctacatg
      481 gacgcgatgg cttatgatta catcagcccc acgcgttggg atgaggggat taatgcgctc
      541 accatcaact acgatttttc tggttcacat acactacgtt cagattatgg ttcacaagag
      601 acagatacca gttatctcaa tctgcgcaat ggactgaata ttggaccgtg gcggctacgt
      661 aattacagta ctttaaacac cagcgatggc cgtgcggaat acaactccat tagtacctgg
      721 atacagcgcg atattgccgc gttaagaagc cagattatga ttggtgatac gtggacggcg
      781 agcgatattt tcgacagtac gcaaattcgc ggcgcgcgtt tgtatactga taacgatatg
      841 ctacccgcca gccagaatgg ctttgctcct gtggttcgtg ggattgcaaa gtccaacgcc
      901 accgtcatca ttcggcagaa tggctacgtg atttatcagt cagccgttcc acaaggtgct
      961 tttgagatca ccgatctcaa caccgcaagt acaggtggcg atttggacgt aaccatcaaa
     1021 gaagaagacg gtagcgaaca acgattcacc caaccttatg cttcattggc gattcttaaa
     1081 cgtgaaggtc tgacagatgt tgatgtcagc gtgggtgaat tgcgcgatga agacggattt
     1141 acaccggacg tccttcaggc gcaaatactt catggttttt cccacgggat cactttatat
     1201 ggaggtatgc aggctgctga aaattatggt tctgcagctc tgggtgtcgg taaagatctt
     1261 ggcgctttgg gcgcaatttc tttcgatgtg acacatgctc gtgcgaattt tagccatgat
     1321 gatacagaaa cgggtcagtc atatcgcttt ctctattcaa aactatttga cgacacagac
     1381 actagcttgc gcctggttgg ctatcgttac tccaccgagg gctactatac cctcaatgag
     1441 tgggcatcgc ggcgcaacag ccctgaagac ttttgggaaa caggtaaccg acgtagtcgc
     1501 gtggagggaa cgctaacgca gtcgttgggg agagattatg gcaatttata cctgacatta
     1561 agccggcaac aatactggca taccgatgat gtcgaacgat taatgcaatt tggctacagc
     1621 agtagctgga agcgtctctc gtggaacgtc tcctggagtt attccaatac tgccagacag
     1681 gggacgggga acaaccatgc cagtgataac accagtgagc agatctacat gctctcttta
     1741 tctgttcctt tatcgggctg gtggggtaat agttacgcca cctattctgt ttcgcaaaac
     1801 gataattccg gtagctcaca tcaactcgga ctcagcggta cggcgctgga aagaaataac
     1861 ctttcatgga atttaatgca gtcctataac agtcatgatg atgaggttgg cggtaatatg
     1921 tccctgacct atgatggctc ttatggcacg gtgaacggca gctataacta cagccaaaat
     1981 tcccagaggc tgaattatgg tatcagaggg ggaattctgg cacacagcga aggggtaacg
     2041 ttaagtcagg agttaggtga aactattgct cttgttaaag cacctggggc cgccaggtta
     2101 gaaatagata atatgcgcgg tgctgcgacg gactggcgcg gctatacggt caagacacag
     2161 ctaaaccctt atgatgaaaa tcgggtagca atcagcgata actatttctc gaagtcgaat
     2221 atagaacttg ataataccgt cgttacgatg gttcccacgc gtggtgcagt ggttaaagcg
     2281 gagtttgtga ctcatgtggg ttatcgcgtt ctcttcagag tgttaaatgc aaatggtaaa
     2341 ccggtacctt ttggagccat tgctgcgata caagatgcaa gtttggcaga ttcaggaatt
     2401 gtcggtgacc gtggcgaact ttatctttct ggtctaccag aaaaaggaca ggttacgtta
     2461 tcctggggag aaaacgcctc aacaaaatgc atcttcaatt attcattttc gacaccagaa
     2521 agtgagagcg gattaattga acagggtgtg acatgtcatt aaaaggattt ttatgaacaa
     2581 gtatatcaaa cagtggtgct ttgctgtgtt tatgctctcg ttaagtagtg tagcactt
//
LOCUS       DAJTUI010000224         2618 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTUI010000224 DAJTUI010000000
VERSION     DAJTUI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.228"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..545)
                     /locus_tag="OO464_004138"
     CDS             complement(<1..545)
                     /locus_tag="OO464_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229047.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
     gene            complement(1207..2196)
                     /locus_tag="OO464_004139"
     CDS             complement(1207..2196)
                     /locus_tag="OO464_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229048.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2280..>2618)
                     /locus_tag="OO464_004140"
     CDS             complement(2280..>2618)
                     /locus_tag="OO464_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229049.1"
                     /translation="EAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAERIAK
                     ELASQKERTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADL
                     ESGGNWMDSL"
BASE COUNT          682 a          611 c          626 g          699 t
ORIGIN      
        1 attccatcca gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc
       61 gtagacatat aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg
      121 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
      181 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt
      241 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgagtttcg
      301 tctttggtat agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaagacga
      361 taattaggat acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccctg
      421 gaattaagaa tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg
      481 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa
      541 tccatcaatc gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt
      601 attttacctg agttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa
      661 atggggaagt gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga
      721 aaatcagata cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag
      781 ggagcctacg acaaatcgaa gacaaccagc taacctattg tttcttaaag acaggtgaat
      841 ccagttggtt gtagtgatag tcacacattt agagatagaa ggtcaagact tgaagcacgt
      901 aatattccct ctaaaggaat agaactcacg gtctgactac ccggcccttt tggtctggct
      961 caggtcaggc tatgggggct tttggctcgc tctttatatt agatgaggtc tcacattttt
     1021 cagtcaaaac tcatcgtcta atagcccata gccagaccac cagtcccagc tttagcagga
     1081 agaccacgag atagactttg tgtaggtcca tttggccctc ctactttctg tattcacaaa
     1141 ctagattact agtacaaaat aaaggccaga catccagttc ggaagtctga ccaatagtcg
     1201 ttattgttag ttagcttctt tagtgaagct caggcgtttg actacaggca ggacagcagt
     1261 agccgagcca gtacccatct tgatggtcgt agcagcttca gctacaacaa acaccttgcc
     1321 gagaatctca aggtcgtgag actgggagtc tccatcacgt gaaaggacca cagtaatgct
     1381 ctcgccatcg tgacggaagc aaccgaagct cagagccgta aaacgcttag ccatcatgaa
     1441 cacgccacgg tcttcaccgt tgtaataggc gacgtcagtc cccagcatat cgacgatagc
     1501 gtcttcacca ccgaagccct gcttagcagc cagacgggag agagccttga ggtgtgactc
     1561 atgggcgata atgccgtagt cagaaacctc agaaccgaaa gaagactccg tgttctcgta
     1621 cagggcatcc atgatagcgt cagccttagc ggtcaggcta ccagtcaggg ccggaagttc
     1681 tacggtcttc agcagctttg aggactccag cacgtcaacc atcttagagt taatggtacg
     1741 aatcacgtta tcgcagatgt tcagtgcgtc ctgctggagc gtcccctgag tgaaaccagt
     1801 ggtcgacttc tggtcccaaa gctttgtttg gtgagacacg aggatcgcct gttcaatcgg
     1861 ctcaacggtg atttcagtgc cagacggagc ggtcacaact tcaggtcgct taccaccacg
     1921 gacagggacc agcacagagc ggtcagccag agtgtgagca atgattggag tcggtaagct
     1981 gttacgggtc ggcaggactt ctacagggaa cgggatgtta tcttgagcgt ctttaagtac
     2041 accgttcagc atagccttaa cagtcttagc taactggagg gcatagacct gagggtcggc
     2101 gttagtcggc agaccttcat tgacctgaat gttgtcggta tcggtgatgc gtactggctc
     2161 aatgccaaac tggaaagcgt ttttagtcat attcattata attaatctcc ttgagtcaaa
     2221 tacttgaagt ccctaacagg cagtcaggga ccgtgtgttc tcagatgagg gacgcttgct
     2281 tacaagctgt ccatccagtt accgcctgac tcaaggtcag cgacgatagc ggcacgtgag
     2341 cgaccagcgg aatgagtagc gctgcggagg ttttcaccat aggtccggcg ttcagctttg
     2401 acctgttcgc ggcgctgtag ctctttttgc cattcgtctt cggtcatgaa catttcgcgg
     2461 gtacgctctt tctgagacgc tagctcttta gcgatacgct cagcttcagc tcgtttgttc
     2521 atctggcggc gttggtaggc ggtcatcagg tcattactga agaagttacc aatggtcacg
     2581 ccagcttcag ccagagcttc cagtaactcc atcgcctc
//
LOCUS       DAJTUI010000225         2610 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTUI010000225 DAJTUI010000000
VERSION     DAJTUI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.229"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            6..224
                     /locus_tag="OO464_004141"
     CDS             6..224
                     /locus_tag="OO464_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6229050.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="OO464_004142"
     CDS             226..591
                     /locus_tag="OO464_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6229051.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="OO464_004143"
     CDS             588..1253
                     /locus_tag="OO464_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229052.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="OO464_004144"
     CDS             1253..1618
                     /locus_tag="OO464_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6229053.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2073..2228
                     /gene="hokD"
                     /locus_tag="OO464_004145"
     CDS             2073..2228
                     /gene="hokD"
                     /locus_tag="OO464_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6229054.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          739 a          544 c          668 g          659 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg
     2101 ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg aaagacctct
     2161 gcgaggtacg aatccgaacc ggccagacgg aggtcgctgt cttcacagct tacgaacctg
     2221 aggagtaaga gaccaggcgg gggagaaatc cctcgccacc tctcatgtgt caggcatcct
     2281 caacgcaccc gcactaaacc cgcttcggcg ggttttttgt tgcgtgctga atacgcaggg
     2341 tgaaaaataa ccatatattt gattatatac acaacaaaaa ataaaagtca ttgtacttgc
     2401 acattaaaca atcaaatata cggcgtgaaa taaatattta tcagattaat atttttgtct
     2461 ctatgtggat ataaccgttt gtacttataa acctgggggc atcgtggaaa aaataaagaa
     2521 actatttagt agcaaatacg cagtcatacg tcgtgatgac ctgtcagtta tagtcgaaat
     2581 ggattacttc cctgaaaccc caaaatcaat
//
LOCUS       DAJTUI010000226         2507 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTUI010000226 DAJTUI010000000
VERSION     DAJTUI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.230"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..959)
                     /locus_tag="OO464_004146"
     CDS             complement(<1..959)
                     /locus_tag="OO464_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6229055.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
     gene            complement(1558..1806)
                     /locus_tag="OO464_004147"
     CDS             complement(1558..1806)
                     /locus_tag="OO464_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6229056.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2161..>2507)
                     /locus_tag="OO464_004148"
     CDS             complement(2161..>2507)
                     /locus_tag="OO464_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6229057.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          642 a          466 c          608 g          791 t
ORIGIN      
        1 tgctcttcat gttcaaaagc atgccatatc tgtgatacat cagattgttt gttttccggg
       61 aaccatgctg tcacggcatc agccaggggg cgatggagtg tattctgttg tccgtcactc
      121 atggagaagt aaatctgcgg gccgtggtag tccggcgaag atgttaatct ttgcagggat
      181 tgcagagcat gggatgacaa tgggttatca ctaatatcta ttgaacattc attcctcaga
      241 ttaagaatgc tttccgggat atgacttatc tgatttatgt caaaataacc gttccttaaa
      301 ttttccggca ggtatggaat atcggttaac atattgtcga ttgcccatat ggagtgaatg
      361 ttgtgaggta gaggggggag ttcactaaga agattcccta ttgcgctaat ctcttgtaaa
      421 gaaaatggta aaggtggtaa aactgccagc ccattacatg atacatctaa tgtttccagt
      481 agttctggta aaacaggaag agaacataat tgattatttg agacatgaag ctccttcaaa
      541 gatatgggga gtgtgggtag tgtgattagt tgattgtggg acgcattcaa taatttaagt
      601 ccttgaggca aagcaggcag ttcaataagt ctgttatagc tgacattaag ttgtgtaagg
      661 gacgcaggca atggggagat taagcttaaa ttatttttac ttatattaat tgatttaatt
      721 cccgggggga tttcaggtaa tgttgtcagg cctaattcag acaagtccag gttcgtctct
      781 tggttttgta gacatgatac tagtcgctga aaagcgatgt ctcgttgttc ttcttgtatg
      841 cggttatttt tccattcagt ccaatgggtt agataatttt catatgcgcg gctagtgtcg
      901 attgaataag tggagaacga atttgaaatt aatctgtgat tgttatttgt cgggagcata
      961 aatatcaggg tgtattcttt atgttgagag gtgttggatt cttttttatc tgctttgcaa
     1021 tgttattggt tcccttcagc agtagacagg aacttctgga ggaggcggat atatagtttg
     1081 tgtaagcata ttaactccat atgttatata ttgaagaact tctgctttac actatcctaa
     1141 actggatcgg tttaatgtaa aaaaacgata ctttttgggg gggggcaatt tttccttaag
     1201 gtatgaaaaa aggagctgaa gctatataat agctattaat gccactggta tcagtgaatc
     1261 atgtatataa aaaactgtgt taaggaggct gtccattagc ggttttttat tcttctggaa
     1321 ccgcggcatc tttgcgtatc tttttgtctt ctcaccgtct ggtcggtgtt ttgctgagac
     1381 tgctaatttc ctgtctttgc tagtgctgta ctggtactgc ccaatcatga ttggcggggg
     1441 cgggcggagt tgagaccgca gacacgtcgt atgcaagaac gtgctgcggc tggctggtga
     1501 acttccgata gtgcgagtat tgaatgattt ccagccgtta ctgattttac gtgctaatta
     1561 gtgaacaaac cactcgtcag cagactccca ggtatctttc agagtttcct gaacaaaagt
     1621 tttagctgaa tctttatcgg cggtgcgcgt aacagaaagg ccatcgttgc tggtggcttt
     1681 tacgatcacc tctacatcgt cataacgttt actgatgcgt cgggttaatt cttcctttaa
     1741 cgcatccaca gcaccggttg gcattttagt cattttctct ttggctatgc agatttcaaa
     1801 acgcataaaa atccctctgc actgtgtttt tatacagtat tatttttaac tgtatggata
     1861 aacagtgtca agagtgaggg aaagtgattt tttggggcat gcatggggca aaaatatttg
     1921 ttgtggggca ttttggggca tgattgggac actttgttac atatgaactt tgccgatttt
     1981 catatgacat ggcaatccaa cgctttgatt attctaaaga aaacacatgc tcttgggcgt
     2041 tctttagtga tttttaaaaa tcccgtatca cgcagttaaa gtggcgggca tactcttcaa
     2101 ggctggtgat ccccaaccgc acccatttag gatgtgacca ttgcggtaga ccgatgtaaa
     2161 tcataacgcg tgaaggatct cttccacgct acgcacacgg gcgatgcgcg ggtagatatg
     2221 attaatgctg ttattgtgtt gctcggcgct ggcggcacta caggcgtctt cggcaatcac
     2281 cagattaaag ccaagttccc aggcattgcg ggcggtggat tcaacaccga tattggtcga
     2341 gatcccacat aacactattg tatcgatacc tcggcggcgc agttgcaact ccagatccgt
     2401 accgtaaaac gcaccccact gacgtttgat gatttcgata tcactgtcag ttgcacctaa
     2461 tgcagctgga tgttgccacc agttttcggg caacacttta gcggggg
//
LOCUS       DAJTUI010000227         2467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTUI010000227 DAJTUI010000000
VERSION     DAJTUI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.231"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..378
                     /locus_tag="OO464_004149"
     CDS             <1..378
                     /locus_tag="OO464_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6229058.1"
                     /translation="NLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            336..1013
                     /gene="bglJ"
                     /locus_tag="OO464_004150"
     CDS             336..1013
                     /gene="bglJ"
                     /locus_tag="OO464_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR6229059.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1051..1839)
                     /gene="fhuF"
                     /locus_tag="OO464_004151"
     CDS             complement(1051..1839)
                     /gene="fhuF"
                     /locus_tag="OO464_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6229060.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            1980..2216
                     /locus_tag="OO464_004152"
     CDS             1980..2216
                     /locus_tag="OO464_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6229061.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2255..2341)
                     /locus_tag="OO464_004153"
     tRNA            complement(2255..2341)
                     /locus_tag="OO464_004153"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2305..2307),aa:Leu,seq:cag)
     gene            complement(2376..2462)
                     /locus_tag="OO464_004154"
     tRNA            complement(2376..2462)
                     /locus_tag="OO464_004154"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2426..2428),aa:Leu,seq:cag)
BASE COUNT          633 a          563 c          636 g          635 t
ORIGIN      
        1 aatctggtca agaccgtacg ttccggcaat acgcacgcag agcgtatcag cgccatgctg
       61 acctctccgg caatgactga aactcatgat tttagctatc gctccgtcat tctcactctt
      121 tcagagcgca aggtactgcg gctattaggt aaaggatggg gcatcaacca gatagcttca
      181 ttgcttaaga aaagtaataa aactatcagc gcccaaaaaa acagtgcgat gcgtcgactg
      241 gcaattcaca gcaacgctga aatgtatgca tggataaata gcgcgcaggg tgcaagagaa
      301 cttaacttgc cttctgttta tggagatgcc gcagaatgga acacagccga attaagaaga
      361 gaaatgtcgc actcatagaa aaatgcgtca tgagtagtat cggtattgag agtttattca
      421 gaaagtttgc gggcaaccct tataagctcc atacctatac cagtcaggag tcatttcagg
      481 atgccatgtc gcggatctcg tttgcggcgg tcattttttc tttttctgcc atgagaagtg
      541 agcgcagaga gggattatct tgcctgactg agctggcgat taagtttccg cgtacccggc
      601 gtttagttat tgtggatgat gatattgaag ctcggctgat tggttcattg tcgccatcac
      661 cgctggacgg tgtattaagt aaagcgtcaa cgctggagat ttttcatcag gaactctttt
      721 tgtcattaaa tggtgtacgt caggcgaccg accgactgaa caatcagtgg tacattaacc
      781 aaagccggac gttaagcccg acggagagag aaatattgcg ctttatgtcg cgtggctact
      841 caatgacaca aattgccgag cagcttaaac gtaatatcaa aacgatccga gcgcataaat
      901 ttaatgtgat gtcgaaactg ggcgtcagtt ctgatgcagg gttgttggag gccgaagata
      961 ttctgttatg tatgcggcat tgcgaagcaa gtaatgtgtt gcatccctat taatccgcat
     1021 gatgccgggt ttacttcccc ggcagtgctt tcatttcagc gtacaatagc cacattgctg
     1081 cacatccggt aagcgataac gctggcagca agtgcggcgc accagcaggc cgtcgcgcag
     1141 taccacggta cgccagagtg gattatcttc accgttcgtg agcgttttct caaaaaagag
     1201 ggcatggcgc agcgattcaa cagtagcctc gccgagcagt tgcttcatct cagtgagata
     1261 ccagttgatc aaataaccgg tattactcca gataagtttg ccgttgatct ctccggtcgc
     1321 ttctagtgct tgcacaaccg gaaccagcgc ctggctgatt aacgtttcca ttcgctgctg
     1381 cggcgaatgt ggtgttgcgt ttttatcttc acacacatcg acccagaaac aggcgacgcg
     1441 tccggtttcg tgaaactcag catggaaatg ttccggcgac acatctaatg ctttttcctg
     1501 cgtcagtagc gccagcatta atggtggcac catcaggccg atataccatt gtgcccatag
     1561 tgagatcagc ggtttgttct cgcggatcat cgttggttgg ttgcgataga tatgatcgga
     1621 atagaccgcc agtagagaac ttagcgcatt cggtgatgac cattgcgcca gcgtcatggc
     1681 gttaagcgga gcgggttcat ccaggcggat aaactccagc aaatgttcac gatgtttggc
     1741 gatcatcgcc cgtacggctt gtgcaagccc agcatcctgc ggctggagat gcgttcgcca
     1801 gatgacatct tcatagagcg gtgcggaacg ataggccata atcgtgatag taatctaaat
     1861 gataatgatt gctaatccta gcgataggtt tactcgatag caagggattt ctctggcttg
     1921 caaatgataa aaattatcat atgatattgg ttttcattat caatgaaaga gatgaaacca
     1981 tgttgcaacg tacgctgggc agtggctggg gagtgttgct gccgggattg ctgattgcag
     2041 ggctgatgta tgcggattta tcgccggatc agtggcggat tgtcattctg atgggattag
     2101 tattgacgcc ggtgatgctg tatcacaaac agttgcggca ttacattttg ctaccatcgt
     2161 gcctggcgct tattgctggc atcatgctga tgataatgaa tttgaatcag ggatgacaaa
     2221 acagggaaga aacaagaaag gaagtaaaga taattggtgc gagggggggg acttgaaccc
     2281 ccacgtccgt aaggacacta acacctgaag ctagcgcgtc taccaattcc gccaccttcg
     2341 cacagtcatc ttactttttt tgatatcgcc tcgtttggtg cgaggggggg gacttgaacc
     2401 cccacgtccg taagaacact aacacctgaa gctagcgcgt ctaccaattc cgccaccttc
     2461 gcccagt
//
LOCUS       DAJTUI010000228         2439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTUI010000228 DAJTUI010000000
VERSION     DAJTUI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.232"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..>2439
                     /locus_tag="OO464_004155"
     CDS             <1..>2439
                     /locus_tag="OO464_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6229062.1"
                     /translation="MFNGTKTWPGNKVIETFESMGLRFGRDVNAYTSYDETVYQVSLP
                     TTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAHQDAKWRTSQARRPFLL
                     ANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDIDSKEALALIKDN
                     LSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYYRLPMVQVNDEQSFVEQ
                     AEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQSLFFRVNARDDNMQDAA
                     NALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLRMLTSRLASSSLNNT
                     PFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNELAAKKALSPAAI
                     LALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTSLTLSNGAKVILA
                     KSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGVGELSSSSLKRWSAENS
                     VTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDNIWASLQNAQIQALKTL
                     DQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAADRQLFSSPADITFVIVG
                     NVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTVKEQNEPVAQVSQWKRY
                     DSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSRLSVDPQAKDISHLLAF
                     TCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSLDIQQRSVQQLANTIIN
                     SLIQYDDPAAWTEQE"
BASE COUNT          713 a          569 c          587 g          570 t
ORIGIN      
        1 atgtttaacg gcacaaaaac atggccgggt aataaagtca tcgaaacatt tgagtcaatg
       61 ggcctgcgtt ttggtcgcga tgttaatgcc tataccagct atgacgaaac ggtgtatcag
      121 gtgagtttgc cgactacgca gaaacaaaat ctgcaacaag tgatggcaat cttcagtgaa
      181 tggagtaatg ccgcaacctt tgaaaaactc gaagtagacg ctgaacgtgg cgtaattact
      241 gaggaatggc gtgcccatca ggatgcgaaa tggcgcacct ctcaggcgcg ccgccctttc
      301 ctgctggcta atacccgtaa tttagaccgt gaacctatcg gcctgatgga tactgtcgcc
      361 acggtcacgc cggcacaatt gcgccaattt tatcaacgct ggtatcaacc aaataatatg
      421 acctttatcg tggtcggcga tatcgacagt aaagaagcgc tggcgctgat aaaggataat
      481 ttaagtaagc ttccggctaa caaagcagct gaaaatcgcg tctggccgac aaaagccgaa
      541 aaccacctgc gctttaatat cattaatgat aaagaaaacc gggtgaacgg catcgcactc
      601 tattatcgcc tgccaatggt acaagtgaac gatgagcaaa gctttgtcga acaagctgaa
      661 tggagcatgt tagttcagct gttcaatcaa cgtctgcagg aacgcataca gtcgggcgag
      721 ttgaagacta tttctggcgg cactgcgcgc agcgttaaaa ttgcacccga ttatcagtcg
      781 ctgtttttcc gtgtaaatgc acgagacgat aatatgcagg atgctgcgaa tgcattaatg
      841 gcagagttgg caaccattga tcagcatggt ttttctgctg aagaactcga tgatgtcaaa
      901 tctacccgcc tcacctggct gaaaaatgcg gttgatcagc aagctgaacg tgatttacgt
      961 atgctgacca gtcgcctggc atccagctca ttaaataata cgccgttctt gtcgccggaa
     1021 gagacatatc aactttcgaa acgtctgtgg cagcaaatta ccgtgcaaag tctggcggaa
     1081 aaatggcagc agttaagaaa gaaccaggac gcattttggg agcaaatggt aaacaatgag
     1141 cttgccgcca aaaaagcatt gtctcctgcg gctatcctgg cgctggaaaa agagtacgcc
     1201 aacaaaaagc tggcggctta catcttccca ggcagaaatt tatcgttaac agtagacgct
     1261 gacccacagg cggaaattag cagcaaagaa acgctggctg agaatctgac atcattaaca
     1321 ctttccaatg gtgccaaggt tattctggca aaatccgcgg gtgaagagca aaagctacaa
     1381 attattgccg tatcgaataa aggcgattta agtttccctg cgcagcaaaa atcacttatc
     1441 gcgctggcaa ataaagcagt tagcggaagc ggcgttggcg aactctcctc ttccagcctg
     1501 aaacgctgga gtgcggaaaa ctcggtaacc atgagcagta aagtcagtgg catgaatacg
     1561 ttgctctccg ttagcgcgcg gactaataac cctgaacctg gtttccagtt gattaaccag
     1621 cgaatcaccc acagcacgat taacgacaat atttgggcat cgctacaaaa tgctcaaatt
     1681 caggcgttga aaacgctcga ccagcgtcca gcggagaaat tcgcccagct gatgtatgag
     1741 acgcgctatg ctgatgaccg cacgaaatta ctgcaagaaa atcagattgc acagtttact
     1801 gccgcagatg cgctggctgc cgatcgccag ttgttttcat ctccagcgga tatcacgttt
     1861 gtcattgtcg gtaatgtcgc agaagacaaa ctcgtggcgt taattacgcg ttacttaggc
     1921 tcaatcaaac actctgattc gccattagcc gcaggtaaac cattaactcg cgcgacggac
     1981 aacgcatcgg ttactgtaaa agaacaaaat gaacctgtgg cacaggtttc acagtggaag
     2041 cgttatgatt cccggacacc tgttaatctg gcgacgcgta tggcgctcga tgcttttaac
     2101 gtcgcactgg caaaagatct acgcgttaat attcgtgaac aggcatctgg ggcatacagc
     2161 gtttcttctc gcctctcggt tgatcctcag gctaaagata tcagtcattt gctggctttt
     2221 acttgtcaac cagaacgaca tgatgaacag ttaacgttag cgaatgaagt gatggttaag
     2281 cgtttgacta aggggatcag tgagcaagaa ctgaacgaat accagcaaaa cgttcagcgc
     2341 agcctcgata tccaacagcg tagcgttcaa caattagcga acactattat aaatagtctt
     2401 attcaatatg acgatcctgc agcatggact gagcaggag
//
LOCUS       DAJTUI010000229         2394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTUI010000229 DAJTUI010000000
VERSION     DAJTUI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.233"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..965
                     /gene="proB"
                     /locus_tag="OO464_004156"
     CDS             <1..965
                     /gene="proB"
                     /locus_tag="OO464_004156"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6229063.1"
                     /translation="VTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLF
                     SIYGIHVGQMLLTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDN
                     DNLSALAAILAGADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVS
                     GLGTGGMSTKLQAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENR
                     KRWIFGAPPAGEITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDI
                     AHGVSRYNSDALRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            977..2230
                     /gene="proA"
                     /locus_tag="OO464_004157"
     CDS             977..2230
                     /gene="proA"
                     /locus_tag="OO464_004157"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6229064.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT          563 a          606 c          699 g          526 t
ORIGIN      
        1 ttgtgacgtc gggcgcgatc gccgccggac gtgagcacct gggttacccg gaactgccag
       61 cgactatcgc ctcgaaacaa ctgctggcgg cggtagggca gagtcgactg attcaactgt
      121 gggaacagct gttttcgatt tatggcattc acgtcgggca aatgctgctg acccgtgctg
      181 atatggaaga ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgata
      241 acaatatcgt tccggtaatc aatgagaacg atgctgtcgc tacggcagag attaaggtcg
      301 gcgataacga taacctttct gcgctggcgg cgattctggc gggtgccgat aaactgttgc
      361 tgctgaccga tcaaaaaggt ttgtataccg ctgatccgcg cagaaatccg caggcagaac
      421 tgattaaaga tgtttacggc attgatgacg cactgcgcgc gattgccggt gacagcgttt
      481 caggcctcgg aactggcggc atgagtacta aattgcaggc cgctgacgtg gcttgccgtg
      541 cgggtatcga caccattatt gccgcgggca gcaagccggg cgttattggt gatgtgatgg
      601 aaggcatttc cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa aaccgtaaac
      661 gctggatttt cggtgcgccg ccggcgggtg aaatcacggt agatgaaggg gcaactgccg
      721 ccattctgga acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg actggcaatt
      781 tctcgcgtgg tgaagtcatc cgcatttgca acctcgaagg tcgcgatatc gcccacggtg
      841 tcagtcgtta caacagcgat gcattacgcc gtattgccgg acaccactcg caagaaattg
      901 atgcaatact gggatatgaa tacggcccgg ttgccgttca ccgtgatgac atgattaccc
      961 gttaaggagc tggccgatgc tggaacaaat gggcattgcc gcgaagcaag cctcgtataa
     1021 attagcgcaa ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg ccgatgaact
     1081 ggaagcacaa agcgaaatca tcctcaacgc taacgcccag gatgttgctg acgcgcgagc
     1141 caatggcctt agcgaagcga tgcttgaccg tctggcactg acgcccgcac ggctgaaagg
     1201 cattgccgac gatgtacgtc aggtgtgcaa cctcgccgat ccggtggggc aggtaatcga
     1261 tggcggcgta ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc tgggggttat
     1321 tggcgtgatt tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc tgtgcctgaa
     1381 aaccggtaat gcggtgatcc tgcgtggtgg taaagaaacc tgtcgcacta acgcggcaac
     1441 ggtggcggtg attcaggacg ccctgaaatc ctgtggctta ccggcgggtg ccgtgcaggc
     1501 gattgataat cctgaccgtg cgctggtcag tgaaatgctg cgtatggata aatacatcga
     1561 catgctgatc ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac agtcgacaat
     1621 cccggtgatc acaggtggta taggcgtatg ccatatttac gttgatgaaa gtgcagagat
     1681 cgctgaagca ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca catgtaatac
     1741 ggttgaaacg ttgctggtga ataaaaacat cgccgatagc ttcctgcccg cattaagcaa
     1801 acaaatggcg gaaagcggcg tgacattaca cgcagatgca gctgcgctga cacagttgca
     1861 ggcaggccct gcgaaggtgg tggcggttaa agccgaagag tatgacgatg agtttctgtc
     1921 attagatttg aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc atattcgtga
     1981 acacggcaca caacactccg atgcgatcct gacccgcgat atgcgcaacg cccagcgttt
     2041 tgttaacgaa gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt ttaccgacgg
     2101 cggccagttt ggtctgggtg cggaagtggc ggtaagcaca caaaaactcc acgcgcgtgg
     2161 cccaatgggg ctggaagcac tgaccactta caagtggatc ggcattggtg attacaccat
     2221 tcgtgcgtaa ataaaaccgg gtgatgcaaa agtagccatt tgattcacaa ggccattgac
     2281 gcatcgcccg gttagtttta accttgtcca ccgtgattca cgttcgtgaa catgtccttt
     2341 cagggccgat atagctcagt tggtagagca gcgcattcgt aatgcgaagg tcgt
//
LOCUS       DAJTUI010000230         2282 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTUI010000230 DAJTUI010000000
VERSION     DAJTUI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.234"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..694)
                     /gene="yehT"
                     /locus_tag="OO464_004158"
     CDS             complement(<1..694)
                     /gene="yehT"
                     /locus_tag="OO464_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR6229065.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLK"
     gene            complement(691..>2282)
                     /gene="btsS"
                     /locus_tag="OO464_004159"
     CDS             complement(691..>2282)
                     /gene="btsS"
                     /locus_tag="OO464_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6229066.1"
                     /translation="LMQVTVRLPHKFLCYIVFSIFCIMGTWFGLHIDDSIANTRAIGA
                     VMGGLLGGPVVGGLVGLTGGLHRYSMGGMTALSCMISTIVEGLLGGLVHSILIRRGRT
                     DKVFNPITAGAVTFVAEMVQMLIILAIARPYEDAVRLVSNIAAPMMVTNTVGAALFMR
                     ILLDKRAMFEKYTSAFSATALKVAASTEGILRQGFNEVNSMKVAQVLYQELDIGAVAI
                     TDREKLLAFTGIGDDHHLPGKPISSTYTLKAIETGEVVYADGNEVPYRCSLHPQCKLG
                     STLVIPLRGENQRVMGTIKLYEAKNRLFSSINRTLGEGIAQLLSAQILAGQYERQKAM
                     LTQSEIKLLHAQVNPHFLFNALNTIKAVIRRDSEQASQLVQYLSTFFRKNLKRPSEFV
                     TLADEIEHVNAYLQIEKARFQSRLQVNIAIPQELSQQQLPAFTLQPIVENAIKHGTSQ
                     LLDTGRVAISARREGQHLMLEIEDNAGLYQPVTNASGLGMNLVDKRLRERFGDDYGIS
                     VACEPDSYTRITLRLPWRDEA"
BASE COUNT          569 a          622 c          538 g          553 t
ORIGIN      
        1 ttttcagata acggcggctg accggcacgg ttaagccatt acgcaaaatc aactcggcct
       61 ggccgttatc ttccagacga atctcctgta aatgcgcgag gttaaccaga tactgacgat
      121 ggcagcgcag tagtggtgta cgactttcca aggtacgtaa tgtcaattcg gtaaagccct
      181 ctttcccttc gtggctggta acgtagacac cgctcatccg actgctgaca aatgccacat
      241 ctttcatttg cagcaaataa atccgactat gccccgtaca agggataaat ttcagcgcct
      301 gttgattttc cggtaacagc gaaacatcct gcttgctgcg ctcctgacgc aatcgcgcca
      361 gcgttttctc cagtcgcgct tcatcaattg gcttcagcag ataatcaaag gcatgttctt
      421 caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa aacaatatac gggcgatgtt
      481 ccgggtcaag catccccacc atttccagac cactgatgcg cggcatctgg atatcgagaa
      541 acagcacatc cgggcgcagt ttatgcaccg cgccgatccc ttccacggcg tttgaacact
      601 ctccaacgat ttcaatatcg ctctgctcct gcaaaaatat acgcaggttc tcccgtgcta
      661 acggttcatc atcgacaatt aagactttaa tcatgcctcg tccctccatg gtagtcgtaa
      721 cgttattcgg gtgtaactat caggctcaca ggcgacgctt attccatagt catcgccaaa
      781 ccgttcacgt aaacgcttat ccaccagatt catccccagc ccactggcat tggttaccgg
      841 ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa tgttgcccct cacgtcgggc
      901 gctgattgcc actcgccctg tatccagcag ttgtgatgtc ccatgtttaa tggcgttttc
      961 cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg gataattctt gcggaatagc
     1021 aatgttgacc tgcaaccgcg actggaagcg cgccttttca atttgcagat aagcgttcac
     1081 atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc cgctttaagt ttttgcggaa
     1141 aaaagtggaa agatactgca ccagctggct ggcctgttcg ctgtcgcggc ggatcaccgc
     1201 tttaatggtg ttaagcgcat taaacaaaaa atgaggattc acctgggcgt gaagcagttt
     1261 gatttctgac tgggtgagca tcgctttttg ccgctcatat tgccctgcaa gaatctgcgc
     1321 cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt attgaactga ataaccggtt
     1381 tttggcttca tacaatttga tggtgcccat cacgcgctga ttttcgccac gcaacggaat
     1441 taccagcgtc gaccccagtt tgcattgcgg atgcaaagag caacgataag gtacttcgtt
     1501 gccatcagcg tagaccactt caccggtttc aatcgctttt aaggtgtaag tcgaagaaat
     1561 cggtttgccg ggtaaatggt ggtcgtcacc aattccggta aaggccagca atttctctcg
     1621 atcggtaatc gcgactgcac caatatccag ctcctgatac agtacctgag ccactttcat
     1681 gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc gtcgaggctg ccactttcag
     1741 cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc gcgcgtttat cgagcaatat
     1801 acgcataaac agcgccgcgc cgacggtatt ggtgaccatc attggcgcag caatattact
     1861 caccagacgc accgcatctt cataaggtcg ggcgatcgca aggatgatca gcatttgcac
     1921 catttcagcg acgaacgtga cggcaccggc ggtaatgggg ttaaagactt tatcagtgcg
     1981 cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc aatccttcaa cgatggttga
     2041 gatcatgcaa cttagcgcgg tcatgccccc catcgaatat cgatgtaagc cgccggtcag
     2101 accaaccagc ccaccgacga ccggaccgcc gagtaagccg cccattaccg cgcctatcgc
     2161 acgggtattg gcaatagaat cgtcaatgtg caacccaaac caggtgccca tgatgcagaa
     2221 gatggaaaag acgatgtagc agagaaattt atgcggcaga cgaaccgtga cctgcattaa
     2281 cg
//
LOCUS       DAJTUI010000231         2248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTUI010000231 DAJTUI010000000
VERSION     DAJTUI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.235"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            651..2159
                     /gene="ltrA"
                     /locus_tag="OO464_004160"
     CDS             651..2159
                     /gene="ltrA"
                     /locus_tag="OO464_004160"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6229067.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          574 a          556 c          659 g          459 t
ORIGIN      
        1 ggcatggatg cttaaatgaa ccgcgagtct gtctggaata ttgaaccggt aactcacgat
       61 gagaaaccca acaatcccac cgggtgtgac ggtggagaac ctgagcggca gtgacctgcg
      121 gcatgcccgc agggtgatgt aacccgctga caacggggat tgaggcgaga tcactaagcc
      181 gagatgatcc tcaaggttaa gtactgaaag gctgaagaac atgaacccgt taatccgcct
      241 ctgtgggttg aaaacgtcac cacggcctac gtgatctgac aggccgtgca ggaggaactg
      301 gcagtgatac gtaagcactg ccggtcgaag gtgttttgac atgtatgcga aacaccgggg
      361 cagcagcgtc tatcacactt gcgttgctga cttctgccaa cttgcggcaa gcaaggataa
      421 agagtgcgac gggcagcctc ctcagtatgc ctgagtccag gcaggtaaac cggggaaggt
      481 cagcgacgga tgttaagggg gcatggctcc gatgacgcgc tggctggcgg agcttccgta
      541 gtagtccgcg atggggaaag cccattacat ggcgaaggga agcagtttga atgtgtttgc
      601 gacgtgaatt aactgaccta acgaggtgaa gacctttgat aatcagcgaa atgcaacgca
      661 agcttgccac atgggcagcc accgatccgt ccctacggat tcaacggctg ctgcgtctga
      721 taacacaacc agaatggctg gctgaagcgg cgcggatcac gctttcatca aagggggccc
      781 atacccccgg cgttgatggc gtgaacaaaa caatgctaca ggccagactg gctgttgagc
      841 tgcaaatcct cagggatgaa ttactctcag gccactacca gcccttgccc gccagacggg
      901 tttacatccc taaaagcaac ggcaaactgc gaccactggg tatccccgcg ttgcgggatc
      961 gtattgttca gcgggccatg ctgatggcga tggagccgat atgggagagt gattttcata
     1021 cgctctcata tggcttccgg cctgagcgca gtgtccacca cgcgatccgc acggtgaaat
     1081 tacagctcac agactgtggt gaaacccggg gacgctgggt gattgaaggc gacctgtcca
     1141 gttacttcga caccgtacat catcgactgc tgatgaaggc tgtacgccgc aggatcagtg
     1201 acgcacgttt catgactctg ctgtggaaaa ccatcaaggc gggacatatc gatgtcggtc
     1261 tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt tatatcgccg ctattatcga
     1321 acatcatgct gaatgagttc gatcaatacc tgcatgagcg ctacctgagc gggaaagcca
     1381 gaaaagatcg gtggtactgg aataacagta tccaacgggg ccgaagtacg gcggtcagag
     1441 aaaactggca gtggaaaccc gcggtggcgt actgccgcta tgccgatgat tttgtcctca
     1501 tcgtcaaagg caccaaagca caggcggaag ccatcaggga ggagtgtcgg ggtgtgctcg
     1561 aaggcagtct gaaactcagg ctgaacatgg ataagactaa aatcacccat gttaatgacg
     1621 gctttatctt tctggggcac aggatcattc gcaaacgcag tcgttatggc gagatgcgag
     1681 tggtctcaac gatcccgcag gagaaagcca gaaacttcgc cgcatcgctg acagcactgt
     1741 tatcaggcaa ctacagtgaa agcaaagtcg atatggctga acaactcaac cgaaaactga
     1801 aaggctgggc catgttctat cagttcgttg attttaaggc caaagtcttc agttatatcg
     1861 accgtgtcgt gttctggaag ctggctcact ggctggcccg caaataccgt acaggtatcg
     1921 cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg tcagagcaaa acgtgggttt
     1981 tatttggtaa aaccaatcac ggcaagctca gcggcgaaat actgtaccgg ttggtggggc
     2041 aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa tccctatctg aggacggaga
     2101 ccagaaacac gtatacatcg cgctttacag aagtggcaat ggcgttcgcc agcatttaaa
     2161 tggagagccg gatgcgctga aaggtgcacg tccggttcgg ggaggagagg cagggaaata
     2221 gtccgactac gccctgcctc ttactcta
//
LOCUS       DAJTUI010000232         2240 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTUI010000232 DAJTUI010000000
VERSION     DAJTUI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.236"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(121..546)
                     /gene="arsC"
                     /locus_tag="OO464_004161"
     CDS             complement(121..546)
                     /gene="arsC"
                     /locus_tag="OO464_004161"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR6229068.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(559..1848)
                     /gene="arsB"
                     /locus_tag="OO464_004162"
     CDS             complement(559..1848)
                     /gene="arsB"
                     /locus_tag="OO464_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6229069.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1902..>2240)
                     /gene="arsR"
                     /locus_tag="OO464_004163"
     CDS             complement(1902..>2240)
                     /gene="arsR"
                     /locus_tag="OO464_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR6229070.1"
                     /translation="PIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISR
                     HLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQN
                     CSGDSKNICS"
BASE COUNT          595 a          615 c          561 g          469 t
ORIGIN      
        1 agtaagttgg cagcattacc tgaatttgca cggttttgta ggtcggataa gacgcgttag
       61 cgtcgcatcc gacataaaca aagcgcactt taccaactat cggcagtgga tatcgccgct
      121 ttatttcagg cgcttacccg cttcatcaac cactttctcg ccatcttcct tggagaatgc
      181 gcctttttgc gcatctggca gaatttccag caccacttct gaagggcggc acaggcgagt
      241 tcccagcggc gtcaccacaa tcgggcgatt aatcagaatc gggtgctgaa gcataaagtc
      301 gattaaccga tcgtcagtaa atttatcttc cgcaaggccc agctcctcat acggttcgac
      361 gtttttacgc agcagcgcgc gtacggaaat ccccatatcg gcaatgagtt tgaccagttc
      421 atcgcgcgtt ggcggagttt ccagataatg gataatggtc ggttctgtgc cgctgttgcg
      481 gatcatctcc agcgtattac gcgacgtgcc gcaggccggg ttgtgataaa tggtaatgtt
      541 gctcatatca gtatctcatt acaaagtgaa agagagacgt agcgccagcg cagccagcgt
      601 cacaaacagc acaggcaggg tcatgataat ccctgtacgg aagtaatatc cccagctgat
      661 agtcatattc ttctgcgaaa gtacgtgcag ccagagcagc gttgccaggc taccaatcgg
      721 ggtaattttc ggccccaaat cgcagccaat cacgttggca taaaccatcg cttctttgat
      781 aacgccagat gccgtgctgc catcaatgga caacgcgcca accagtaccg tcggcatatt
      841 gttcataata gaagagagga aggcggtgag gaatccggtg ccgagcgtcg cggcccacag
      901 gccgttatcc gccagcacgt tgagtacgcc agaaagatat tccgttaatc cggcattgcg
      961 caggccataa accaccagat acatgccgag cgagaagatg acaatctgcc agggggcacc
     1021 gcgcaggact ttacccgtat taatcgcatg accgcgttta gcgacgacaa ataatatcag
     1081 tgcgcccaca gctgcaatgg cgctcaccgg aatgccgagc ggttccagga cgaaaaatcc
     1141 caccagcaga agcagtaaaa caacccagcc agttttgaac gtagcaggat ctttgatcgc
     1201 ttctgcggga gatttcagca gcgccatatc gtagttctgc ggaatatctt tgcgaaaata
     1261 gagatgtagc atcaccagcg tggcaacaat cgcggcgata tccaccggca ccatcaccga
     1321 ggcgtattcg cgaaagccga ggccaaagaa atcagcggaa acgatattca ccaggttgga
     1381 gacaataagc ggcaggctgg cggtatcggc aatgaatccg gccgccatca cgaacgccag
     1441 cgtcgtgcct ttactgaatc ctaaagccag cagcatggcg atgacaatcg gcgtcaaaat
     1501 aagcgccgcg ccatcattgg caaacagggc cgcaacggcg gcaccgagca ggacaatcca
     1561 ggtaaacagc aagcgaccgc gtccattgcc ccagcgtgaa acgtgcagcg ctgcccattc
     1621 aaaaaagccg gactcatcca gcagcaggct gatgataatg acggcaataa acgccgccgt
     1681 cgcgttccag acgatattcc acaccaccgg aatatcaccc ggatgaacca cgcccgtaac
     1741 caacgccagt accgcgccga gcgttgcact ccagccgatg cctaaacctt tcggctgcca
     1801 gataaccaat acgatggtca ggacaaagat agcgcctgcc agtaacataa tgcctcccgg
     1861 ataaaacaca tgtgcaaatc catatgtgtt tagctaaatt tttaactgca aatgttctta
     1921 ctgtctccgg aacagttttg tctggccagg ttgcggacaa ttgcctgaat cttttcctgt
     1981 tcacatcgcc aggcttcatc aataattttc gccgcccatg ctggaatatg cggcgataag
     2041 cggtaatgaa cccacttacc ttgcttgcgg tccagcaata gcccgctttc acgcagcaat
     2101 gccagatggc gggatatctt gggctgcgac tgatcgaggg cagtgcagag atcgcagacg
     2161 cataactctc ccagttcgct aagcagtaaa acgatgccca gacgggtttc atcagcaaga
     2221 attttgaaca attggatggg
//
LOCUS       DAJTUI010000233         2215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTUI010000233 DAJTUI010000000
VERSION     DAJTUI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.237"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..419
                     /locus_tag="OO464_004164"
     CDS             <1..419
                     /locus_tag="OO464_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6229071.1"
                     /translation="TADNLPENKESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKM
                     FYRPAHLKGRVNDAWKSLEFKRSDHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAP
                     GEHKQLPLPASGGKNVKWAAINDYGGSSGTETRPLQ"
     gene            448..>2215
                     /locus_tag="OO464_004165"
     CDS             448..>2215
                     /locus_tag="OO464_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6229072.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          662 a          523 c          486 g          544 t
ORIGIN      
        1 aaacagctga taacctgcct gaaaataaag agtcgctgtt ctacattaat gttcgtgcca
       61 ttccagcaaa gaaaaaatca gatgatgtta atgctaacga gttgacgctg gtatttaaaa
      121 cgcgggtcaa aatgttttat cgccccgcac acctgaaggg acgggtaaac gatgcgtgga
      181 aatcactgga atttaaacgt agtgaccatt cactcaatat atataaccca actgaatatt
      241 acgtcgtatt tgccggactg gcagtcgata aaaccgatct cacaagcaaa attgaatata
      301 tcgcgcccgg agaacataaa cagttaccac ttccagcatc tggcggaaag aacgtgaaat
      361 gggctgcgat caacgattat ggcggcagtt ccgggacaga aactcgtcca ctgcaataaa
      421 aaatataaaa acacaggcca tcagggaatg ccacaacgac accaccaggg acataaacgc
      481 acaccgaaac agttggcgct cattatcaaa cgttgtttgc cgatggtgct cactggcagc
      541 ggcatgcttt gcactaccgc taacgccgaa gagtattatt tcgaccccat tatgctggaa
      601 actacaaaaa gtggtatgca aacaaccgat ctgtcacgtt tttcaaaaaa atacgcacaa
      661 ctaccaggaa cttatcaggt tgatatctgg ctgaataaaa agaaggtttc acagaaaaaa
      721 attacattta ccgccaatgc agagcaactt ctgcagccac agtttacggt agaacaacta
      781 cgtgagctgg gtattaaggt ggatgaaatc ccggcgctgg ctgaaaaaga tgacgatagc
      841 gtgatcaact cgcttgaaca aatcattccc ggtacagctg ctgaatttga tttcaatcat
      901 cagcgactta atttgagcat tccccaaatt gcactgtacc gtgatgcaag aggttacgtc
      961 tccccttctc gttgggacga tggtatacca acgctgttta ccaactactc gtttacaggt
     1021 tctgataacc gttaccgcca gggcaatcgt agccaacgac agtacctgaa tatgcaaaat
     1081 ggtgctaatt ttggcccctg gcgattacgt aactattcta cgtggacacg caacgatcag
     1141 gcgtcaagct ggaacactat cagtagttat ttacaacgtg atatcaaggc gttgaagtct
     1201 cagttgcttc tgggagaaag cgccaccagc ggcagtattt tttccagcta cgcctttact
     1261 ggcgtgcaac tcgcttccga cgataatatg ttgccaaaca gccagcgcgg atttgcccca
     1321 acggtacgcg gtatcgcaaa cagtagtgca atcgtgacta tcaggcaaaa tggttatgtg
     1381 atctatcaaa gcaacgtgcc agcgggtgcc tttgaaatta acgatctcta cccctcttcc
     1441 aacagcggcg atttagaagt cacgattgaa gaaagtgacg gtacacaacg tcgctttatc
     1501 cagccttatt cttcattacc catgatgcag cgacctgggc atctaaaata tagcgcgacc
     1561 gctggacgct atcgcgctga tgcaaacagt gatagcaagg aacccgaatt tgctgaagcc
     1621 acggcaatat atggtttgaa taatactttt acgctgtatg gcggcctgct cggttctgaa
     1681 gattattatg cgctggggat cggtatcggc ggcacacttg gcgcactggg cgcgttgtcg
     1741 atggatatca acagagctga cacccaattc gataaccagc actcttttca tggctatcaa
     1801 tggcgtactc agtacatcaa agatatcccg gaaaccaaca ccaatatcgc tgttagctac
     1861 tatcgctata ccaacgatgg ctattttagt tttgatgaag ccaatacccg caattggggc
     1921 tataacagtc gccaaaaaag tgaaattcaa ttcaacatca gccagacaat atttgatggg
     1981 gtaagtctgt atgcctccgg ttcacagcaa gactattggg gcaataacga gaaaaacagt
     2041 aatatctctg ttggggtttc cggccagcaa tggggaattg gttacagcct gaattatcaa
     2101 tacagccgct acactgatga aaataatgac cgtgcactct ctttgaatct cagtattccg
     2161 ttagaacgct ggtggatttg cccctatatt tccagacatc tgttatcact taacc
//
LOCUS       DAJTUI010000234         2184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTUI010000234 DAJTUI010000000
VERSION     DAJTUI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.238"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..2037
                     /gene="sat"
                     /locus_tag="OO464_004166"
                     /pseudo
     CDS             1..2037
                     /gene="sat"
                     /locus_tag="OO464_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
     gene            complement(2081..>2184)
                     /locus_tag="OO464_004167"
     CDS             complement(2081..>2184)
                     /locus_tag="OO464_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6229073.1"
                     /translation="EYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          785 a          389 c          436 g          574 t
ORIGIN      
        1 tacggcatga tgatttataa aaatgataaa acatttcgta atcttgaaat attcggagac
       61 agcggctctg gagcatactt atatgataac aaactagaaa aatgggtatt agtcggaaca
      121 acccatggta ttgccagcgt taatggtgac caactgacat ggataacaaa atacaatgat
      181 aaactggtta gtgagttaaa agatacctat agtcataaaa taaatctgaa tggcaataat
      241 gtaaccatta aaaacacaga tataacatta caccaaaaca atgcagatac cactggtact
      301 caagaaaaaa taactaaaga caaagatatt gtgttcacaa atgggggaaa tgttctgttt
      361 aaggataatt tggattttgg ttgcggtggt attatctttg acgaaggcca tgaatataac
      421 ataaacggtc agagatttac atttaaagga gcaggaattg atatcggaaa agaaagcatt
      481 gtaaactgga atgcattgta ttccagtgat gatgttttac acaaaatagg ccccggtact
      541 ctgaatgttc aaaaaaaaca gggggcaaat ataaagatag gtgaaggaaa tgttattctt
      601 aatgaagaag gaacatttaa caatatatac cttgcaagcg gaaatggtaa ggtaatacta
      661 aataaagata attcccttgg caatgatcaa tatgcgggga tattttttac taaacgtggt
      721 ggcacgctag atttaaatgg acacaatcag acttttacta gaattgccgc cactgacgat
      781 ggaacaacaa taactaactc agatacaaag aaagaagccg ttctggcaat caataacgaa
      841 gactcctaca tatatcatgg gaacataaat ggcaatataa aactaacgca caatattaat
      901 tctcaggata agaaaactaa tgcaaaatta attctggatg gtagtgtcaa cacaaaaaat
      961 gatgttgaag tcagtaatgc cagtcttacc atgtaaggcc atgcaacaga gcatgcaata
     1021 ttcagaagca cagcgagtca ttgctccctg gtatttcttt gtggaacgga ctgggtcacc
     1081 gttttgaaag aaacagagag ttcatataat aaaaagttca attctgatta caaaagtaat
     1141 aatcagcaga cctcatttga tcagcctgac tggaaaaccg gggtgtttaa atttgataca
     1201 ttacacctga acaatgctga cttttcaata tcacgcaatg ccaatgttga aggaaatata
     1261 tcagcaaata aatcagctat cacaatcggc gataaaaatg cttacattga taatcttgca
     1321 gggaaaaata ttactaataa tggttttgac ttcaaacaaa ctatcagtac taatctatcc
     1381 ataggagaaa ctaaatttac aggtggcatc actgcacata acagccaaat agccataggt
     1441 gatcaagctg tagttacact taatggtgca acctttctga ataatactcc tataagtata
     1501 gataaaggag caaaagttat agcacaaaat tccatgttca caacaaaagg tattgatatc
     1561 tccggtgaac tgactatgat gggaatccct gaacagaata gtaaaactgt aacgccgggt
     1621 ctccactacg ctgctgatgg attcaggctg agtggtggaa atgcaaattt cattgccaga
     1681 aatatggcat ctgtcaccgg aaatatttat gctgatgatg cagcaaccat tactctggga
     1741 cagcctgaaa ctgaaacacc gactatatcg tctgcttatc aggcatgggc agagactctt
     1801 ttgtatggct ttgataccgc ctatcgaggc gcaataacag cccccaaagc tacagttagc
     1861 atgaataatg cgatctggca tctaaatagc cagtcatcaa ttaatcgtct agaaacaaaa
     1921 gacagtatgg tgcgttttac tggtgacaat gggaagttta caacccttac agtggacaac
     1981 cttactatag atgacagtgc atttgtgctg cgtgcaaatc tggcccaagc agatcagtga
     2041 ggtgtactgg caatagcgga cactaccatt tgttcttttt ttaagcagcc atctgatgat
     2101 atttttccct gaaggctgcc ggggagatat tccccagacg agagtgacga cgctgacgat
     2161 tgtagaaaat ctcaatgtat tccc
//
LOCUS       DAJTUI010000235         2159 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTUI010000235 DAJTUI010000000
VERSION     DAJTUI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.239"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            40..1437
                     /gene="ccp"
                     /locus_tag="OO464_004168"
     CDS             40..1437
                     /gene="ccp"
                     /locus_tag="OO464_004168"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6229074.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1648..>2159
                     /locus_tag="OO464_004169"
     CDS             1648..>2159
                     /locus_tag="OO464_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6229075.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFAR"
BASE COUNT          587 a          456 c          556 g          560 t
ORIGIN      
        1 ttctgggtca caataactca ccaccgtgag ggaagaataa tgaaaatggt ctcacgtatt
       61 accgcgatcg gcctggctgg cgtcgcgatt tgctatttag ggttatctgg ttatgtgtgg
      121 taccacgata ataaacgcag taaacaggcc gatgttcagg catctgctgt cagtgaaaat
      181 aataaggttt taggctttct ccgcgaaaaa ggatgcgact attgccacac gccttcggca
      241 gaattacccg cctattatta tattcctggc gcgaaacagt tgatggatta cgacattaag
      301 cttggatata aatcttttaa cctcgaggcc gtgcgtgcgg cactgctggc tgataaaccc
      361 gtttcgcaaa gcgatctgaa taagattgaa tgggtgatgc agtatgaaac tatgccacca
      421 acgcgttata ccgcgctaca ctgggcgggt aaggtgagtg atgaagagcg ggcggaaata
      481 ctggcctgga ttgcaaaaca gcgcgcggaa tattacgcca gcaatgatac tgctccggag
      541 catcgcaatg aaccggtgca gcccatcccg caaaaactgc ctaccgatgc gcaaaaagtg
      601 gcgttgggct ttgcgctgta tcacgatccc cgtttatcgg ctgatagcac catttcatgc
      661 gctcattgcc atacgttgaa tgcggggggc gtcgatggca gaaaaacatc gattggtgtt
      721 ggtggcgcag ttgggccgat taatgcgccg acggtattta actcagtatt taacgttgag
      781 cagttctggg atggtcgtgc ggcaacattg caggatcagg caggtggacc gccgttgaac
      841 ccgattgaaa tggcgtcgaa atcctgggac gaaattattg ctaagctgga aaaagatccg
      901 cagcttaaag cgcagttcct cgaagtttat ccgcaaggtt tcagtggcga aaatattact
      961 gatgccattg ctgaatttga gaaaacatta attacgccgg attccccatt tgataaatgg
     1021 ttgcgtggag atgaaaatgc gctgacggcg caacagaaaa aaggctatca attatttaaa
     1081 gataataaat gtgcaacttg tcatggtggt attattctcg gcggacgttc ctttgaaccg
     1141 ttggggctga aaaaagactt taactttggg gaaattacgg cggcggatat tggtcgtatg
     1201 aatgtgacta aagaagagcg tgataaattg cgtcagaaag tacccggttt acgtaacgtt
     1261 gctttaacgg caccgtactt ccatcgcggt gacgtgccga cgctggacgg ggcggtgaaa
     1321 ctgatgctgc gctatcaggt aggcaaagag ctgccgcagg aggatgtgga tgatatcgta
     1381 gctttcctgc acagtctgaa cggggtgtac acgccgtata tgcaggataa acaataatta
     1441 atttgatcgc ccgaacagga atgtttgggc gatttttatt acgataataa agtctgtttt
     1501 taatattatc atgttaaatg tttatattat aaaaagtcgt ttttctgctt aggattttgt
     1561 tatttaaatt aagcctgtaa tgccttgctt ccattgcgga taaatcctac ttttttattg
     1621 ccttcaaata aatttaagga gttcgaaatg gaccagaagc tgttaacgga tttccgctca
     1681 gaactactcg attcacgttt tggcgcaaag gccatttcta ctatcgcgga gtcaaaacga
     1741 tttccgctgc acgaaatgcg cgatgatgtc gcattccaga ttatcaatga tgaattatat
     1801 cttgatggca acgctcgtca gaacctagcc actttctgcc agacctggga cgacgaaaac
     1861 gtccataaat tgatggattt gtcgatcaat aaaaactgga tcgacaaaga agaatatccg
     1921 caatccgcag ccatcgacct gcgttgcgta aatatggttg ccgatctgtg gcatgcgcct
     1981 gcgccgaaaa atggtcaggc cgttggcacc aacaccattg gttcttccga ggcctgtatg
     2041 ctcggcggga tggcgatgaa atggcgttgg cgcaagcgta tggaagctgc aggcaaacca
     2101 acggataaac caaacctagt gtgcggtccg gtacaaatct gctggcataa attcgcccg
//
LOCUS       DAJTUI010000236         2158 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTUI010000236 DAJTUI010000000
VERSION     DAJTUI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.240"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            80..259
                     /locus_tag="OO464_004170"
     CDS             80..259
                     /locus_tag="OO464_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229076.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            311..505
                     /locus_tag="OO464_004171"
     CDS             311..505
                     /locus_tag="OO464_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229077.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1304..1640)
                     /locus_tag="OO464_004172"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="OO464_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          687 a          443 c          448 g          580 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctgacgaa atcaacatga aaataaccat atatgataat tattataata acggctttaa
     1801 ttggaataca tatattacaa cgtattatat ataattggta ttctgggaac tatattctca
     1861 aaatacagta gaaacgaggt atgtttctgg tggaaaggac agtgggatta aaaagtaaga
     1921 attgataaaa aaaacgccag caacacatag tgccggcgga gggaataccc catggagaaa
     1981 atgtgatgcc tagagcatca tgaatatata attaaaaata gttagcgttg tcaatacttt
     2041 cacaaaaata attttcgtag tatagaaaga tatttttatg catgacctac ctgaatttgc
     2101 tccgggtaga ggttataaat aaaaattgaa tcacgacaaa cacaatattc atagtatg
//
LOCUS       DAJTUI010000237         2127 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTUI010000237 DAJTUI010000000
VERSION     DAJTUI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.241"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..798
                     /locus_tag="OO464_004173"
                     /pseudo
     CDS             1..798
                     /locus_tag="OO464_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            913..1254
                     /locus_tag="OO464_004174"
                     /pseudo
     CDS             913..1254
                     /locus_tag="OO464_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1237..2074
                     /locus_tag="OO464_004175"
                     /pseudo
     CDS             1237..2074
                     /locus_tag="OO464_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          524 a          512 c          643 g          448 t
ORIGIN      
        1 ctgggccttg cccgtagcag ttatttttat caccgggctg cactgaaagc cggtgataaa
       61 tacgccacga tacgtaagat gctgacagat atctttaaca gtaattacca gtgttatggc
      121 tatcgtcgcc tgcatgcgat gctcaggcat gagggggggc ggctatcaga aaaggttgta
      181 cgcagactta tggtggagga acagcttgtc gtcagtcgta accgtcgtcg tcgctacagc
      241 tcatattgcg gagaaatcgg accggctccg gataacctta tcgccagaga ttttaaggcg
      301 gagcaaccta atcagaaata gctgacagat atcacggagt tccagctccc tgcaggtaaa
      361 gtctggctgt caccagtggt ggactgcttc gatggaaaag ttgtgagctg gtctctcagt
      421 acacgccctg atgctgaact ggtcaacact atgctggata gcgctgtcga aacgttaaat
      481 gctggcgaac gaccggtgat acacagtgac agaggtgggc attatcgctg gccaggctgg
      541 ctggaaagag tgaatgcagc aggtcttatt cgctcaatgt cccgtaaagg atgttcacct
      601 gataatgctg catgcgaagg ctttttcggc agactgaaaa cggaaatgta ttatgggcgt
      661 aaatggtcgg gcatcacgcc agaaaagttc atgcagcaag tggatgctta catcagatgg
      721 tataacgagc ggcgtataaa attatcgctg ggtgccgtca gccccaaaat gtaccgccaa
      781 caatgcgggc tggaatgata aagcagtcca ggaaatcgtc cgcatccccc cttcaccatt
      841 tggattgtcc gctccaccca acatgttgtt tccttaaggt tcctacacca caaaggaaac
      901 tctcatgctg cgcagtggat ggctgacgtt gccgacaggc gggaacttcg ccagttcagg
      961 cagacaccgg aacagcgctt cacgcaggaa caggagcatc tgcagccgct gctgggtacg
     1021 gacttcgata tccgtcatgt ctcctgggat ggctatatcg aggttggcgg gaaccgttac
     1081 agtgtgccgg aaagcctgtg tggtcagctg gtatcaatac ggatctcgct ggatgatgaa
     1141 ctgcggatct acagtaatga gcaacaggta gcatcgcacc gtctctgttc ggctgcatac
     1201 ggctggcaga ccgtgccgga gcatcacgct ccactctggc agcaggcttc ggagcgcatg
     1261 gccgaatggc tgggggagat acagaaacgg gtaatcactg tctgtgaccg ggaagcggat
     1321 atctggcatt acctgtatta taaagtcagc cacggacagc gtggtgcgtg ctgcacagaa
     1381 tcgccggctg gaagaggcac ccggcaagct cttcgaactg ccggaagtcc tggcaaccgc
     1441 aggaagccac acgctgaatg tgatgcaaaa aggcgggcgg gcagcccgtc aggcccggat
     1501 gttcatcagt tacagcgaag tcagcataaa aaatcccgac aacagcggcc aggcgctccc
     1561 gctcacgtat gtctgttgcc gggagcaggc agaggacggt gcctgctggc atctgctgac
     1621 gtcaggaaaa gcggcgagtg ccgccgatgc acgacgtatt gtcagccatt acgagcgacg
     1681 ctggctgaca gaggaatacc acaaggcgtg gaaaagtggt ggtacatgga atcgctgcga
     1741 atgcagaccc gggataacct tgagcgcatg gtggttatcc tggcgtttat agcggtacgg
     1801 gtgctgggcc tgcggcaggg aggtgtcagt gaagaaacac aaaatgacag ctgtgagaaa
     1861 atactgacgc cgacagagtg gaaactactg tgggttaagc tggaaggaaa gccgcttccg
     1921 gtgcaggcac cgaccctgaa atgggcctgg ggcgatggca tgacagcaaa cgcacaggtc
     1981 gtcccggttg gggcgtcatg tgggatggct ggtcagactt caggatatgg ttgagggtta
     2041 cctggtgatg aagtctcttg atcaggagct ctgatcaaga gacagcagct taggggggtg
     2101 cccctattaa gttgtcaagc atgttat
//
LOCUS       DAJTUI010000238         2115 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTUI010000238 DAJTUI010000000
VERSION     DAJTUI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.242"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            274..578
                     /locus_tag="OO464_004176"
                     /pseudo
     CDS             274..578
                     /locus_tag="OO464_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            747..2111
                     /locus_tag="OO464_004177"
     CDS             747..2111
                     /locus_tag="OO464_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6229078.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
BASE COUNT          548 a          417 c          486 g          664 t
ORIGIN      
        1 gggttgggga gcaatggaac agtaaacgcc gttaagaagt caatttaaat agccaaaaat
       61 taactagcca atttacattg caggttgtaa tttttaacca ccttttttac gatagccaaa
      121 ttacctatac ccatcccggc cctgccaagc catacccgct atcggcgatg aaaatgcttt
      181 tgtgcgcggt cggagtggtc ccgacgaggg ttttacccga agtcggggcg tatctccgcg
      241 ttagcgggcc gtgagggccg cttacgagcg tgtatgagag acgtacagtg gttttgtccg
      301 ctcacaccag tacatacagg tacggaaata aaaccagtcg aaatgccggt aacgacgatt
      361 actcctgtct ctgatgccgg tggactacaa gattttattt actggcgtcc tgatgcggac
      421 agggctggta tggaacctgt ttatgtgata cttaatgatc ctctggattc cgggcgattt
      481 acgcgtaaac aactaaataa aaatatctta aacatgcaag taattttggt attctcgata
      541 caagaataaa tagtagtaac tactatagct gcaaataatg ttaaaaacat ttctatgatc
      601 atctttacat ttttcatgat tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg
      661 tgataatgca atactttgag tgattatata atgataagta aacaggtttg cgacaattgg
      721 ttatcagtat tacagaagga ttaaatatgt cattattcac agaacctaag gaaattgaaa
      781 gattaccctc tgaagagatt gaacgattat atcccgtcct gcgatacagg gtctttatca
      841 gcattttcct cggatatatg gggtattatt ttgtccgaaa caccaccagt gtgttatccg
      901 gtgtgctgca catgtctgca accgagatcg gcattatctc ctgtgccggt tttttatcct
      961 atggtatatc aaagtttgtt tccggactta tttctgacag aagtaattca aaagtatttt
     1021 tgtctcttgg tttattcctg tccggactgg taaattttct gataggatat attcccggga
     1081 tcatcacatc cgttacgtta ttttcaacaa tgtatttact taacggctgg atccaaggta
     1141 tgggatatcc tcctggtgcc aaaacactgg tgttctggta tgaacatcgg gaaaggatca
     1201 cctgggccac actgtggaat ttatcgcaca atgtcggggg agctctggcc cccgttctga
     1261 ttggtttcag ttttggtttt ttcggcgaca gtgctctgga tcatgcccgg gccgcattta
     1321 tcttcccagg agtactgtgt atggcaatga gtgtgctgat ttactttatt caggtggaca
     1381 gacctgtatc agtggggctt cctcccattg aagaatggaa aggtaacgtc gtttcacacc
     1441 cggctaaagg ccgtgaacaa ggtccgagac tctccattcc tgatatcatc agaaaacata
     1501 tcatccgaaa taataaactc atttactgct gtatctatgg ttcgtttgtt tatattctga
     1561 gatacggtat tgtttcctgg gctcctaagt ttttaagtga cagtctggat gtcggtggta
     1621 aggatatggg aaaactggca tcaatggggg ggggttctgt ttttgaaatc ggtggtgtgg
     1681 ctggtatgct tcttgccgga tacctgtctg tcagactgtt cagaaactca aaaccattaa
     1741 caaatacttt atttctggca cttaccatta ttttattaat cgcgtactgg tacgtgccat
     1801 caggcaatga atatctctgg ctaaactata caatccttat tctgttgggg ctggcagtgt
     1861 atggtccggt gatgtttatt ggtctttatt caatggagct tgttcctaaa gaagctgccg
     1921 gagccgcatc agggctgtca ggcacatttt cctatatctt cggctcaatt gttgcgacac
     1981 tgggaatggg acttgttgtc gattatctgg gatggggggc cacatttatc gtactgatac
     2041 tgtctgctgt ttttgccatc attttcacac tgatgagtcg tgaacgctca ctggagttcg
     2101 aaaaagagta atttt
//
LOCUS       DAJTUI010000239         2113 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTUI010000239 DAJTUI010000000
VERSION     DAJTUI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.243"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(221..385)
                     /locus_tag="OO464_004178"
     CDS             complement(221..385)
                     /locus_tag="OO464_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229079.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1287..1571
                     /locus_tag="OO464_004179"
     CDS             1287..1571
                     /locus_tag="OO464_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6229080.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1559..2044
                     /locus_tag="OO464_004180"
     CDS             1559..2044
                     /locus_tag="OO464_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6229081.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          548 a          442 c          604 g          519 t
ORIGIN      
        1 ttcaaaatga tggacattat ttttgtggaa ccggaggtaa cagaccagac ggtttaaatg
       61 agccggttac tttaaacggg tgatggacag cacctcaaaa gttcagatgg attatatttg
      121 tctgcaatac cccgggtatt ttcgatttgc gaaatggatg gaacgacttg ctcaggggat
      181 agctgatgga gttactgaag taccgaagga ccactaaaaa tcacaggccg ggaatttcgg
      241 cgtttccggc gtaggtgtgc cacgaatgca tccagtggcg tgatgttgga tgcaatgctg
      301 tatgagagat gaggggcggc gtccagacgc agccttcaaa ccgccttgag ctgctgccgt
      361 taagagcggt ggtggtgagc gtcaaggtta aaaggcgcag aacatgtgtc aggtaggtgt
      421 tgcggagatg aacgaaagtg aactgtcgat gaagtgtcga aaggaacctc taggcgatgt
      481 cgaaaatgtg ctatctacgc agcgcatgac gagtccagca gatatctggt actggctggg
      541 cggcatccgg catataggca gcatgaacgc aacacaggct ctcgtacgga acgtgggaac
      601 ctgtcgtctc gatgctaagg gagcggcgcg gagcagaggc actgcaagcg cctgagtacc
      661 gatgcgagac acaggggcag atggcttcgt agtagtgagg aagtgcctgt aatgggcatg
      721 gagcgaaggg ggtcagtcgt tggggctggt ttatcggtca accggatagc cgggaggagc
      781 ccatgagtca gccaaagccg ttcgaggtct ctaagtatgc ggtttggaaa gcataccagc
      841 gggtcaaagc taatcgaggt gcggcaggtg tcgatgggca gtcagtcgag gcgtttgagg
      901 ttaccaatcg aagctatacc cggtaatggc gcaggttgac ctgcacttgg ccaagtggat
      961 tgcccgcaaa cacaagcgag cgcgaggtag tctggtgcgt gcattcgagt ggttactgcg
     1021 aattcgtcac gactgcccga cgctgtttgc acactggtgc ttagcttacg atacttgaat
     1081 ggttcaacga caagagccgt atgacgcgag agtgtcacgt acggctccgt gggagcgtag
     1141 gggggcggtg cccctgcgcc acccgatatc ccctttgagt gcggttacgt ccacagaggt
     1201 aaatgccgct gacgcggcat ctgtaatctg ttactttgtg tatatcattt gctatatatt
     1261 gtcaatatgc tattgttgta aagcaaatga tatacggagg ctttatgaaa tcaggtgttc
     1321 agctcaatct cagagcaaga gaatcacagc ggatactcat tgatgctgct gcagaaattc
     1381 ttcataagtc gcgtacagat ttcattctgg aaatggcctg taaagctgct gaagatgtta
     1441 ttctcgaccg gcgggtgttt aattttaatg acaggcaata tgaagagttc attgaaatgc
     1501 ttgatgctcc ggttgcagat gatcctgcca tcgaaaaatt gctggcaagg aaacctcaat
     1561 gggatgtgta actgcaccag aacctttatc ctcatttcat caggtcgctg aatttgtcag
     1621 cagtgaagct gtacttgatg actggttaaa gcagaaggag ctaaaaaatc aggcaatcgg
     1681 agccacccgg acttttgttg tatgcagaaa aggtacgcaa caaatagtcg gtttttactc
     1741 actggccact ggtagtgtca atcatacaga agctacaggt aaccttcggc gcaatatgcc
     1801 agaccccatt cctgtaatta tacttgcacg cctggctgta gatgtgtcat ttcgaggaaa
     1861 agggcttggc gcggatttac ttcatgatgc agttcgtcgt tgttacaggg ttgccgaaaa
     1921 tatcggtgtg cgtgcaatta tggttcatgc gcttacggaa aacgcaaaac agttttatat
     1981 tcatcatggt tttaagccat caaaaactca ggtgcaaaca ttgtttctta aactccctca
     2041 atagggagta atgccgatca gttaaggatc agttgaccga tccagtggct gtgtaagaat
     2101 ccggaaacgc tca
//
LOCUS       DAJTUI010000240         2083 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTUI010000240 DAJTUI010000000
VERSION     DAJTUI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.244"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            106..1143
                     /gene="curA"
                     /locus_tag="OO464_004181"
     CDS             106..1143
                     /gene="curA"
                     /locus_tag="OO464_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR6229082.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1341..2006
                     /gene="mcbR"
                     /locus_tag="OO464_004182"
     CDS             1341..2006
                     /gene="mcbR"
                     /locus_tag="OO464_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR6229083.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          575 a          441 c          557 g          510 t
ORIGIN      
        1 tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca atcccgtgag
       61 cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg gcaacaaaag
      121 cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt tccggagaat
      181 ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt actgcgcaca
      241 gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc atcttattca
      301 ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt cgtggagtcg
      361 aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg gcaggactat
      421 gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa tccatcgtgg
      481 tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct ggatatcggt
      541 cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt gggggcgacg
      601 gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg tggcgcggaa
      661 aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca ccacgcggac
      721 gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta ttatgaaaac
      781 gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc gcgcattccc
      841 gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc ggatcgttta
      901 cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt tattatcgct
      961 caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg ggtgaaagag
     1021 gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc acagacgttt
     1081 atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc gggtgatgat
     1141 taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct tattttcagc
     1201 tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg ggtggtttat
     1261 gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg gttggacaga
     1321 aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc agtttgactc
     1381 tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa cctggcgcac
     1441 gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct gtgcgtgaag
     1501 cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa gcatttacag
     1561 taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac gaactggaat
     1621 taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca gaacttcagg
     1681 aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa atcattaatg
     1741 tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc ctgtgtgaga
     1801 tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat gaagcgatta
     1861 acccagcgga attacgggag cgcatagaaa actatcatct attactcgct gcgttaaaag
     1921 caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa aatattgcta
     1981 ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg catcaagtaa
     2041 ttactcaaat cgccacgcaa tattcatgcc gacaccataa ttt
//
LOCUS       DAJTUI010000241         2024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTUI010000241 DAJTUI010000000
VERSION     DAJTUI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.245"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..352)
                     /locus_tag="OO464_004183"
     CDS             complement(<1..352)
                     /locus_tag="OO464_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6229084.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILM"
     gene            complement(419..1633)
                     /locus_tag="OO464_004184"
     CDS             complement(419..1633)
                     /locus_tag="OO464_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR6229085.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          629 a          489 c          412 g          494 t
ORIGIN      
        1 gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc attaatgacc
       61 agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc aaaataccgg
      121 aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca ccaaactctg
      181 tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat gacgaacggt
      241 cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc tgaatacgac
      301 gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc atttttagct
      361 ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg aaccgttatt
      421 accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc gggtcatacg
      481 gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca tcgcctgggt
      541 gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt tgttcggaat
      601 agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc agcgccgccg
      661 catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc tgataaagcg
      721 atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg atcgtcgtgc
      781 gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata ctgttaaaca
      841 cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa cattcctggt
      901 tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg ctcttcccaa
      961 agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga tgttcgttaa
     1021 aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga tacttttctg
     1081 aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac gccagtcctt
     1141 tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc acacgaatcg
     1201 ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca atcgtacgcc
     1261 cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc agttgataca
     1321 atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg gcagaaatgg
     1381 ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat tgccagatat
     1441 cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct ttcagataag
     1501 aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa agtccgcttt
     1561 cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa acatctgcag
     1621 caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa cctaatttta
     1681 cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca ttaaaaacaa
     1741 aaatttctac cgaggagaaa aaataccctc aaaaaccacc tgaataatta accattattg
     1801 ttaaaacaac acgttgaata atcacataac aaactccatt aatcattgaa aatgagcaca
     1861 gaaatagaaa ttaattatat tcaattaatt aaaatgtgta ttgcttgatg tatttaaaaa
     1921 tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag gaatgtgaca
     1981 aataatggtt ctggttgggc gttaaattta aggtcaccag aatg
//
LOCUS       DAJTUI010000242         1980 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTUI010000242 DAJTUI010000000
VERSION     DAJTUI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.246"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            61..282
                     /gene="kil"
                     /locus_tag="OO464_004185"
     CDS             61..282
                     /gene="kil"
                     /locus_tag="OO464_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6229086.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYILHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            276..452
                     /locus_tag="OO464_004186"
     CDS             276..452
                     /locus_tag="OO464_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229087.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="OO464_004187"
     CDS             527..802
                     /locus_tag="OO464_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229088.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>1980
                     /locus_tag="OO464_004188"
     CDS             902..>1980
                     /locus_tag="OO464_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR6229089.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKV"
BASE COUNT          607 a          444 c          443 g          486 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatattcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
//
LOCUS       DAJTUI010000243         1969 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTUI010000243 DAJTUI010000000
VERSION     DAJTUI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.247"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..488
                     /locus_tag="OO464_004189"
     CDS             <1..488
                     /locus_tag="OO464_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229090.1"
                     /translation="GSLYVGVYGGSPADIAAAIWRKAPPGIDMNGNTTFTVADKEYDP
                     PYPEYVISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAFNGTDGGLRARVAS
                     VVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQE
                     A"
     gene            490..1095
                     /locus_tag="OO464_004190"
     CDS             490..1095
                     /locus_tag="OO464_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR6229091.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1082..>1969
                     /locus_tag="OO464_004191"
     CDS             1082..>1969
                     /locus_tag="OO464_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6229092.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQ"
BASE COUNT          469 a          453 c          554 g          493 t
ORIGIN      
        1 ccggttcgct ttatgttggc gtgtacggcg gcagcccggc agatatcgcg gcggccatct
       61 ggcgtaaggc tccgccgggt attgatatga atggcaacac aacgttcact gttgcagata
      121 aggagtacga tccgccgtat cctgaatacg tgatcagctg gcagacactc aaacccgtca
      181 gtctgcatgt cagtgtgacg ctgaaaaaaa gtgactacct gccttcagat attacccgac
      241 aggtacagca atccgtgctg gacgcattta atggtacaga tggtggtctg cgggcaagag
      301 ttgcctctgt tgtctctgcc gggcgctact atgccggcat ttacaaaacc gatccggaac
      361 atattgatat tctgggcctt actgtgagcc gtgatggttc gtcatggaca acggctgtca
      421 ctttcgggat agatgagatt ccggttctgg atgtgtcgga tatcagtgtg aaactccagg
      481 aggcataacg tgcagaatgt ggctgccact gtgcttgcgc agtatgctgc cagcccccga
      541 ctcaatgccc tcattaacag ctttaacgca gcgctttccc ccgacagttt tatcagtgat
      601 ttttatggtc ttatctggaa catcgatacc gcagaaaagt atggtcttga tgtctgggga
      661 aagattgtgg gtgtcagtcg ctggctgacg gtaaaggacg attttaatta cctgggcttc
      721 agcgagtcca ggatggacac cccggtaatg gatgatccct gtccgtttaa tcaggcaccg
      781 ttttacaacg gaaaatcgga tacccggact gttgacctgt ctgatgctgt ataccggcgg
      841 ctgatactga tgaaagccat gtcgaacatt actgactgtt ccgttccgga tattaacctg
      901 atgctgagat ttatgttcgg aaaaaaacgc cgggcttatg ttctgaataa tggcgggctg
      961 aggatgagtt acgtctttga gtccgcgctc tcgttggcag aactggcgat tatccagtcg
     1021 tcgggtgcac tgccatcccc gccgggtgtt tatgtttcag tagttttaaa ggagtcccgt
     1081 aatgaaggcc agtgataaac cccgccagct ggcggtcccc tttgcgagta ccggagataa
     1141 aaaccgtatc ccggacaagg cgacacagca gaccagagag agcggtaatg ctgcgtatga
     1201 ttcaggtttt cctccggtga ccatgacagc ggtctcagcg ggaggtatac cgccacacgg
     1261 caaggatttt aacggtctga tgtacgatat taccgcagca atacggttcg cccaggctgg
     1321 cggtttgtac acgtataatg ccggttttgc gggggccatt ggtggatatg caaaaggagc
     1381 cattctcgcc ggagtcgcaa caacagcggt ctggctgaat accacggacg ataacctgac
     1441 cgatcctgaa ggctccgaca gtgcgggctg ggtaaatctt cttgaggatc cgaaaaggat
     1501 attcctgcgg cagaagaaca atctgtcaga ccttcagaat aaagggacgg cacgggataa
     1561 tcttcaggtt tacagtaaag agcagtcaga tcaacgctat gttcatcggg aaggcgataa
     1621 aataaccgga gagctgaaaa tccgtggtgt taatgcgctg aggattttca acgaagcttt
     1681 tggcctgatt tttcgtcgtt cggaagagtg cctgcacctt attcccacca gtgaaggtca
     1741 gggggaaaat ggcgatattg gtcccctgcg cccgttcacc attaatttgc ggacgggtga
     1801 aatatccatg tcgcataaag tgtctgttgg cggtggttcg caggtcaatg gtgcgctggg
     1861 tatcggcgtt cagaacgccc tgggggggaa ttcaattgtt cttggtgata atgacaccgg
     1921 atttaaacag aatggagacg gtattctgga tgtttatgct aatagtcag
//
LOCUS       DAJTUI010000244         1939 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTUI010000244 DAJTUI010000000
VERSION     DAJTUI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.248"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..139)
                     /gene="ospE1"
                     /locus_tag="OO464_004192"
     CDS             complement(<1..139)
                     /gene="ospE1"
                     /locus_tag="OO464_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HCR6229093.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IY"
     gene            complement(321..722)
                     /locus_tag="OO464_004193"
                     /pseudo
     CDS             complement(321..722)
                     /locus_tag="OO464_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1151..1939
                     /gene="virF"
                     /locus_tag="OO464_004194"
     CDS             1151..1939
                     /gene="virF"
                     /locus_tag="OO464_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR6229094.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          607 a          342 c          361 g          629 t
ORIGIN      
        1 cgtatatagc tgctttttta ttgaaaacag tatatccatc tgtagtaaca gtagaagata
       61 ataatacagg atttgatact tcaaggatga tattttcttg tttttgtgga aggcaaggga
      121 atatagtttg tgtaagcata ttgcttcctt atgttatatt ttagtaaaga atttattttt
      181 tacattatag tcggctaaat gaagtttact gtaaaaaaac gatacttttt tcgtaagcga
      241 tgagatgtgg agccagaaaa atggctgcgt tacgtcattg aactgtcctt gacccacgat
      301 attcagacag tcgttgtatc ttaagttgac attacccgct gacgtcgata ttcccttggt
      361 gagagataac ccaacgcact atgcggatgg ttttcattgt aatgctcgat cgccactgca
      421 aggttatgca atgccgttct tggtttcggc atgaacgcga tgcagtcttc cttcatcgtt
      481 ttcatgaacc gttctgctat tccattactc tgagggctac tgactgccgt cgtgcagggc
      541 tccagattca actctctggc gaactgccgt gtttcatgag ccgtataagc cgaaccgtta
      601 tcggccactg gattggccgt tccggtcctt tatcgccata tcgctcctga catgacatcc
      661 tgcaccgttg ctttgtcata atctcccgtg cctgctgccc agtctatggc ttcacgatcg
      721 caatatggaa cgtaacgctg tcatcgggag aatcgtcaat gcggcattcg gttaacgctt
      781 accaatatca acaccaaccg gatttgtgga gcctccagtc tgaaggggct ttatgcgttc
      841 cgtataggat attatgatgc tggagttttg caaagctcca ctcctttccg aacatttaca
      901 cagatattgc ctaagaaaag tgaaaccata atatggttat agtccctttc agtgcaaata
      961 cttagcttgt tgcacagaga aatagaagct gcataagctc tttcttcaaa aaatgtaaat
     1021 aaagttaaat ataggaaaaa ttacttaatc tatcttaata acggaaagat tttgtataca
     1081 atcactgtta cagaaatttc ttagttactc tgtaaacact aaatatagtt tggtatattc
     1141 tgttgaattt atgatggata tgggacataa aaacaaaata gatataaagg ttcgcttgca
     1201 taactatatt attttatatg caaaaaggtg ttcaatgacg gttagctcag gcaatgaaac
     1261 tttgactatc gatgaagggc aaattgcttt tatagagcga aatatacaaa taaacgtctc
     1321 cataaaaaaa tctgatagca ttaatccatt tgagattata agccttgaca gaaatttatt
     1381 attaagcatt attagaataa tggaaccaat ttattcattt caacactcct attctgagga
     1441 gaaaaggggg ttaaacaaaa aaatattcct cctctctgag gaggaggttt ctatcgattt
     1501 gttcaaatct ataaaagaga tgcctttcgg caaaagaaag atctatagtt tagcttgcct
     1561 tttatcagct gtttctgatg aggaagcttt atatacttcg atatcgatag cttcttctct
     1621 tagtttttct gatcagataa ggaagattgt tgaaaaaaac atcgagaaga gatggcgtct
     1681 ttctgatatt tcaaataact tgaatttatc agaaatagct gttagaaaac gattggagag
     1741 tgaaaaatta acatttcaac aaatccttct tgatattcgc atgcatcatg cagcaaagct
     1801 tttattgaat agtcaaagct atattaatga tgtatcaaga cttatcggaa tatcaagccc
     1861 atcttatttt ataaggaaat ttaatgaata ttatggtata actccaaaga aattttactt
     1921 atatcataaa aaattttaa
//
LOCUS       DAJTUI010000245         1913 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTUI010000245 DAJTUI010000000
VERSION     DAJTUI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.249"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(53..1702)
                     /locus_tag="OO464_004195"
     CDS             complement(53..1702)
                     /locus_tag="OO464_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6229095.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          506 c          491 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAJTUI010000246         1846 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTUI010000246 DAJTUI010000000
VERSION     DAJTUI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.250"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(1..800)
                     /locus_tag="OO464_004196"
                     /pseudo
     CDS             complement(1..800)
                     /locus_tag="OO464_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(851..1426)
                     /locus_tag="OO464_004197"
                     /pseudo
     CDS             complement(851..1426)
                     /locus_tag="OO464_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1430..1830
                     /locus_tag="OO464_004198"
                     /pseudo
     CDS             1430..1830
                     /locus_tag="OO464_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          398 a          531 c          466 g          451 t
ORIGIN      
        1 ccccgctcac caccatggtc agagccgaag aacaggaagt ttttacgacc cagactgacc
       61 gcccgcaggg cattttcagc gatgttgttg tcgatttcca cccagccatt actgcagtac
      121 acgttcaggg ccttccactg tttcagtagg tatgcgaacg cttttgccgt atccgagtga
      181 cgcgacagtg ttttcatctg ttgctgtatc cggtcataca gtgactgcat cagtggcgcg
      241 gctcttgctt ttcttgccgc cagacgctgt tctgctgaac atccccggac ttctgcctcg
      301 atggtataca gttcaccgat acgctgcagg gcttccgtgg tgatgtcggt tggcactctt
      361 gcatgcacat cgtggatttt tctccgggca tgggccatac acgcagcttc cgttattctg
      421 ccggattcgt ataacgcccg gtaaccaccg taagcatctg cctgaagcac accgctgtaa
      481 ccggctaggt gattctgtgg atggatacct ttccggtccg ggctgtacgc gaaccagacc
      541 gccgggggca tctgtgaacc ggcgttacgg tcatcacgga catagaccca cagccgggca
      601 gttcttgttt taccactgcc cggctcctgg accggaacgg ggatatcatc cgcatgaact
      661 ttaccgggca tcagtacata ctggcgcagg acgtcataca gcggctccag cagttcagca
      721 acggcaccgg tccagcgacc cagtgtggca cggctcagct ccacgccctg acgacggtat
      781 atttctgact ggcggtataa ctgtcaacga cggatgaaaa gtggctcttc cgcaacctgg
      841 gtggagattt tcatgccagt ggactcatta ccctctgcct gagtagttca aatcctgctc
      901 gaccatacat ctggcgtttc agcatcttct tcaggcgatt tacatgacct tcgacaacac
      961 cattactcca gcgactactg attgcctcac atatagctgc agcatcagct tccatccccg
     1021 ccgccacgcg ccgaaattct gctgaaccgc tttcgtggac tcgagtgaac caactgctta
     1081 gctgtgattt attttgggtt ttcagaatac ggtagaactc gagtgccagt tgctgcgcta
     1141 ttttcagctc aggttctttt tcacacatca gactaataaa tcgggaagca taattttctt
     1201 catccctggt tattctccag ggcatcaacc agcggcttac ccgggagact gaaggaagtc
     1261 tgacggcagt agtaaccggt gggttccagc ctttacgcca tttggcaacc gcatccctga
     1321 cgatggtttc actgcctgta aatccccggg ccaccatttc ccgccatatc cggctggcgt
     1381 tataattacc actttctcgt tgctctttta accactccct ccatggtgtc agcgaaaaac
     1441 gaaaccgctg ttgaaatccc tggaaagctg gttgcggaaa agatgaagac ccagtcgcga
     1501 cactcagagt tggcgaaggc gttcgcgtac gcacttaacc agtggccggc actgacgtac
     1561 tatgcgaacg atggctgggt ggaaatcgac aacaacatcg ctgaaaatgc cttgcgggcg
     1621 gtcagtctgg gtcgtaaaaa cttcctgttc ttcggctctg accatggtgg tgagcgggga
     1681 gcgctactgt acagcctgat cgggacgtgc aaactgaatg acgtggatcc agaaagctac
     1741 cttcgccatg tgcttggcgt catagcagac tggccggtca accgggtcag cgaactgctt
     1801 ccgtggcgca tagcactgcc agctgaataa cacatccccg tcaata
//
LOCUS       DAJTUI010000247         1840 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTUI010000247 DAJTUI010000000
VERSION     DAJTUI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.251"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(61..573)
                     /locus_tag="OO464_004199"
     CDS             complement(61..573)
                     /locus_tag="OO464_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR6229096.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            768..1532
                     /locus_tag="OO464_004200"
     CDS             768..1532
                     /locus_tag="OO464_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229097.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1483..>1840
                     /locus_tag="OO464_004201"
     CDS             1483..>1840
                     /locus_tag="OO464_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCR6229098.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEG"
BASE COUNT          541 a          398 c          460 g          441 t
ORIGIN      
        1 cagattctgt tgtcagagga atgggggacg ctggattcgc aagagctttt tatgggcagt
       61 ttagctaaaa aaaggcaaca cgaagctaaa actaccaaca taaagtgctg accagctagc
      121 agctgaaatc agactcactg tatacacttt cactggatga agttgcaaca ttcctgccac
      181 caaaggagca atgtaacgca gcacggcgat aaagcgcgaa gtgaaaagta ttgacacaga
      241 gttattttgc aactgaaggc gaacccgttc tattgtattg gaacggctcg atattataca
      301 tgcaagttga gggatatgcc caattattat accaagatgg taattaacga tagtaccgca
      361 ccatgcgcca ctcatgaccg tgattccagc ttcccatggt gaaagagtcg tcttgctgac
      421 tgtaattaca gtggccatca taattgatgc tggtggcaat atcgccgaaa tgagtaaagt
      481 ggattttgtg aacgctatta taaaaagaag cccccatagg cgatatggat gaagtacaaa
      541 atagttcatc aaagcattta tccactccat caatgtcttt ccttctgtga atttttcttt
      601 gctatttaac cgtgatacga cttaatttag gcttaaacac cttacattta caaggtactc
      661 ctgtgggggg ccctgccacg gagcgtcgca ggagtgagtg atgaggaaga attaaagcaa
      721 cctattgtga caggcagcga taacagtaaa tcacaggaag cataatcatg gcaaaaccgg
      781 actgggaggc catcgaatcg gcataccggg ccggagtcct tagcctccgt gatataggcg
      841 agaaatacgg cgttacagaa ggggctatca ggaagagggc caaaaagctt gattgggcac
      901 gcagtggcgg tacgcaggtt tgcaaaaatg gtacgcaaaa aaggaaagtg cgtaccagca
      961 gaaagcctgc cattactggc cttacacaaa aaagtacgca actaaaaaca gaatctacac
     1021 cggatacgaa accgatacgc ggaatgcgta ccgatccccc gactaaccca ttccaacccg
     1081 gtaaccagca ggcattaaaa cacggtggtt atgcccgtcg ccttctgctc aaagatgagg
     1141 tgatagagga cgctaaagcg ttgacgctcg aggacgaatt atttcgcctt cgtgctaaca
     1201 accttgtcgc tgcagagaat attggtcggt ggttggtgtc gctggaagat gctaatgggg
     1261 accaggaaag gaagatgctg attgaaaata tcagcgccgc cgagaaagca atgatgcgca
     1321 atacagttcg tattgagtcc atcgttggca cgcttgcgac ggtaggaaaa atatttgctg
     1381 atacagccta ccgcaaggcc gccactgata aggtgtctct ggaggctgat cgtcttcgcc
     1441 gtgatgcagg tattgatgat ggcaatggag agcgtgacct caatgacttc tactctgaca
     1501 tccaaaccga cgctgaatcc ggctttacgt agtttctgga ctatgcgggc acgtaacaaa
     1561 gtgctttatg gtggtcggtc atcgtcaaaa tcatgggatg ccgctggcat tgccatattt
     1621 ctgtcgaata aatacaccct gcgtttttgt tgtgcccgtc agatccagaa taaaatcgaa
     1681 gagtcggtgt ataccctgct caaaattcag atagacaggt ttggtctgcg gcaccgtttc
     1741 cgtattctga acaacaaaat cattaaccgg gttactggct cggaatttgt tttttatgga
     1801 ttatggcgca acatcgaaga aattaagtca ctggagggga
//
LOCUS       DAJTUI010000248         1792 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTUI010000248 DAJTUI010000000
VERSION     DAJTUI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.252"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            166..1191
                     /locus_tag="OO464_004202"
     CDS             166..1191
                     /locus_tag="OO464_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6229099.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1259..1792
                     /locus_tag="OO464_004203"
                     /pseudo
     CDS             1259..1792
                     /locus_tag="OO464_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          418 a          417 c          460 g          497 t
ORIGIN      
        1 gcggggcgct gtttttatta tttcctgtct acactcagct tattgcgacg cgaaatcgtg
       61 cgcaaaagct ggcactactt ttgcttataa gaggatggcc acagacaggt aaaagacgtt
      121 tcccaaacgt cctataacga taattttcgc ctcacaggaa gcattatgaa aaagatgatg
      181 atagccacac tggctgccgc cagcgtgctg cttgccgttg caaatcaggc gcatgctggc
      241 gcgacgcttg atgccgttca gaaaaaaggt tttgtgcaat gcgggatcag tgatggatta
      301 cctaggttct cttatgccga tgcagacggt aagttttcag gtattgatgt tgatgtttgt
      361 cgtggtgttg ccgctgctgt atttggtgac gacacgaaag tgaaatatac cccactcaca
      421 gcaaaagaac gcttcaccgc tttacagtca ggggaagtgg atttgctctc ccgtaatacg
      481 acctggactt catctcgcga tgccgggatg ggaatggcat ttaccggcgt cacttattac
      541 gacggcattg gcttcctgac gcacgataaa gcggggctaa aaagcgcgaa agaactggat
      601 ggcgctaccg tctgtattca ggcgggtact gataccgaac tcaacgtcgc cgactatttc
      661 aaggcaaaca atatgaagta cacaccagtg actttcgatc gctctgacga atcagcgaag
      721 gcactggaat ctggtcgctg cgatacgctg gcttcggatc agtcgcaact gtatgccttg
      781 cgcatcaaat taagcaaccc ggctgaatgg attgtcttac cggaggttat ctctaaagaa
      841 ccgcttgggc cggtagttcg tcgtggcgat gatgaatggt tctcgattgt acgctggacg
      901 cttttcgcca tgctaaatgc tgaagagatg ggcatcaatt cccagaacgt cgatgaaaaa
      961 gcggctaatc cagcaacgcc tgatatggca catctgttgg gtaaagaagg cgattacggc
     1021 aaggatctga agttggataa taaatgggcc tataacatca tcaaacaggt gagtaactac
     1081 tcggaaattt ttgagcgtaa cgtaggttca gaaagcccgc tgaaaattaa acgtgggcaa
     1141 aataatctct ggaataacgg cggtattcag tacgcgccgc ccgtgcgtta agtcggtgtg
     1201 aggtaacgag cgtcgctacg gcggcgctct cttcagcgtt atgctttccg aggtttctat
     1261 gcctcatcgc cgctcaaccg ttaaaggctc actctccttt gccaacccta cggttcgtgc
     1321 ctggttattc cagatccttg ccgttgttgc tgttgtcggc attgttggtt ggttatttca
     1381 aaacactgta acgaatctca ataatcgtgg cattacttca ggttttgcct ttctggatcg
     1441 cggcgctggc ttcggtattg tccagcattt gatcgattac cagcagggcg acacctacgg
     1501 acgcgttttt attgtcggct tactcaatac gctactggtt tctgcattgt gtattgtgtt
     1561 cgcttctgtt ttgggcttct ttatcggtct ggcgagactt tcggataact ggctgctacg
     1621 aaagctttcc acaatttata ttgagatctt ccgtaatatt gcaaatcttc ttctggtact
     1681 ttgccgtgtt gcgcaatttg cccggaccac gccaagctgt gagcgcgttt gatctggcct
     1741 ttttgagcaa tcgtgggctt tatattccgt caccgcagct gggagacgga tt
//
LOCUS       DAJTUI010000249         1780 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTUI010000249 DAJTUI010000000
VERSION     DAJTUI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.253"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..278)
                     /locus_tag="OO464_004204"
     CDS             complement(<1..278)
                     /locus_tag="OO464_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6229100.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPS"
     gene            416..1336
                     /locus_tag="OO464_004205"
     CDS             416..1336
                     /locus_tag="OO464_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6229101.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1336..1686
                     /locus_tag="OO464_004206"
                     /pseudo
     CDS             1336..1686
                     /locus_tag="OO464_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          481 a          418 c          443 g          438 t
ORIGIN      
        1 gatgggcggt tctgcagatc gctcagggat gtcagggcag aatctatgtc atgcaatata
       61 gggccaagaa cagacaaaag atgctcgccc gcttcagtgg gtacaacgct gcgcgtggtt
      121 cgcgtcagaa gccgcaatcc caatcgttct tctatacggc gcactatctg gcttaaagct
      181 gactgcacca tgctcaggcg ggctgctgcg cgagtgaagc tacgctcctc tgcaacgacg
      241 acaaacgcca tcagatcagc gatttcttca cgcttcattc agtatctcca tacaatctat
      301 taatcattta aatgataggt ctaatcatga tttagggtct aatcaactta atgatcaata
      361 tctatagtga tatcaccaac cgtcaaaacg aaccgacaac tccatgagga actgaatgat
      421 gaataataaa gtcagcttca ctaacagcaa taatccaacc atctctttgt ctgcagtgat
      481 ctatttccct ccaaaatttg atgaaacccg tcagtatccg gcaattgtgg tatctcaccc
      541 aggtgggggc gttaaagagc aaacggccgg aacctatgcc gaaaagctgg cggaaaaggg
      601 atttgtcact attgcttatg acgcatctta tcaaggtgaa agtggcggcg agccgcgtca
      661 gttagaaaac ccatacattc gtactgaaga cgtcagtgcc gtgattgatt acctgacaac
      721 gctttcttat gtcgacaata cccgaattgg tgcgatggga atttgtgccg gtgcaggata
      781 tacagccaat gctgcaattc aggatcgccg catcaaggcc atcggtaccg ttagtgcggt
      841 caatattggt tcgatgttcc gcaatggttg ggaaaacaat gtgaaatcta ttgatgctct
      901 gccacacatt gaagctggct caaacgcccg aaccagcgat attagcagcg aagaatacgc
      961 cattatgcca ctggctccaa tgaaggaggc cgatgcgccg aacgaagaac tgcgtcaggc
     1021 ctgggagtat taccacacac ctcgtgcgca gtatccaaca gcaccgggtt acgctacttt
     1081 gcgcagcctt aaccagatta ttacctatga tgcttaccat atggcggaag tgtacctgac
     1141 tcagccgatg cagattgtgg cgggcagcca ggcagggagc aaatggatga gtgacgacct
     1201 gtacgatcgg gcatcaagcc aggataaacg ctatcacatt gttgaaggcg caaaccatat
     1261 ggatctttat gacggcaaag cctatgtggc tgaagcaatt tcagtattag ctccgttctt
     1321 tgaggaaact ctgtaatgaa acatattcaa atccgcaata gtgatatggc ctggcatatt
     1381 gctgccaata ttcagtttcc accaaatttt gatgagtcaa aacagtatcc ggcgattatc
     1441 agcgtccatc cttttggcag ttgtaaggag cagacgtctg gcaatatcta tggcaaggca
     1501 ttggctgaaa aaggctatgt cgttctcgct tatgacgcca gtttccaggg agaatctggt
     1561 gggtagcctc gttggataga agatccgaca cagcgcgtag aggatattag ccgtgttatc
     1621 gattatgcag taactctgcc atatgttgat gctgaacgca ttggcgtgct gggtgtgtgt
     1681 ggggggtgcc cctattaagt tgtcaagcat gttatgaccc ctgcggggta taaaaagtcc
     1741 cgtcgcgcat catggcgaac agaacgtcgc agcgtcgtct
//
LOCUS       DAJTUI010000250         1764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTUI010000250 DAJTUI010000000
VERSION     DAJTUI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.254"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            114..1718
                     /locus_tag="OO464_004207"
     CDS             114..1718
                     /locus_tag="OO464_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6229102.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          482 a          404 c          449 g          429 t
ORIGIN      
        1 gacctttctt aaagtactcc aacctactga atcctaaggg atacggcttt aagcctcctt
       61 gcagagttac ttactgtaac ccactgtgta accctctcag ggcatgcaga catatgattc
      121 agctatccag tagtcacaaa cttccagcag tctactatct ctaccagcgt aacggcgtct
      181 actacttccg tcttagggtc agacaatcaa acaatgatag aatgacctca atatcattac
      241 ggaccaagga tagacgcacc gctatggctt actcaaggca cataaaggca gcactcaagg
      301 caatacacgc agacagaccc aacgctacct atgaggaaat gagggaacac cttaaggaca
      361 tcgctgagtg tgaactgtct atgggccgtt cagatctctt tgagcctgac atgagggata
      421 tctaccggga ccaatacgga gaacttggtg agagccttac cgatgctctg gctagtgagc
      481 cgctaagcat tgaccagcat cgttacatca atgaggccct gaaggtgcta aaagcctgta
      541 tgaggcgtat cgaagcggga gacagtcagc ctctgataga ttacgtcgat ctttttaatg
      601 atattgatag gcaagataac caagcagact ctgtgtccct atctgttaat gctccagaag
      661 taaaaccaga agttacgcct agcattacca tagcgagcct ctttgaacaa tacgaagcgg
      721 agaactatca gaactggaag cctgcaacct taagggaaaa caaggcgtca cacgctgcgt
      781 taatcgaaat attcgaccat ctgggtctca atgcagacgc taacagggct gacatgctta
      841 gggtccgtga tgtgctccag cagttaccta ggaaccgtaa gcagcgcttc aaggatgtcc
      901 cacttgctga cctgttgagc cgtgaagata agacagattg tctggatgtc gttaccatca
      961 acaacaagta tcttattaag atggctgctg tctttaggtg ggctgtgcgc aatgacttga
     1021 ttaaaaagaa catgactgag ggtctggagc ttaaagtccc tcagcgtaaa gcctcagggg
     1081 cccgtaacgc cttctctacg gagcaggtgg ggcaactact ggtcgcagct aaggcatact
     1141 ctcagaagac ctctggtaag ccgtatcatt attatgttac cgccttggct gctatcactg
     1201 gggcgagact caatgaaata gcccaactac aggtcaaaga cgtcaggacc actgaggcgg
     1261 gaaccgttta tattcatatc aatgaagacg atagtagtct acccggtaag agcatcaaga
     1321 acgctcatag tgaccgctgt gtgcctctgg tcgatggggc ctatggtttt atattggctg
     1381 actttatggc gcttgtagag acccgtagag gcgctgacgg tgatgacgct atggtgttcg
     1441 atggtctgcg tctcatgaag aacggttacg gtgagcaggt gagtaaatgg tttaatcgga
     1501 cgttgttgcc taaagtcctc gtagaccgta gcggcttagc gtttcactcg tttagacata
     1561 cagttgcggc ccagttaaaa caacatgggg tcgagttggc gtatgcacag gcgatcatgg
     1621 ggcatagttc agggtctatt acgtacgacc gttatgctaa agaggtcgag gttgataggc
     1681 tggtcaatgt gatggctgat gtttataagg agacctagag gagaccatag tctaggatga
     1741 cacttactac agacctgtgt ggtc
//
LOCUS       DAJTUI010000251         1741 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTUI010000251 DAJTUI010000000
VERSION     DAJTUI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.255"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(136..603)
                     /locus_tag="OO464_004208"
     CDS             complement(136..603)
                     /locus_tag="OO464_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6229103.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(669..908)
                     /locus_tag="OO464_004209"
     CDS             complement(669..908)
                     /locus_tag="OO464_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR6229104.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1043..1648)
                     /locus_tag="OO464_004210"
     CDS             complement(1043..1648)
                     /locus_tag="OO464_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6229105.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          559 a          383 c          309 g          490 t
ORIGIN      
        1 cggaacaatt tgaaaaccag aaccttgctt aggcctgtgt ccatattacg tgggtaggat
       61 cacaagacct actgctatca caattactga gagaaaaaaa agcatccgat gattcattta
      121 tgctgtccta aaaaattaac aaaacaatgg gaaaacagga tatgtaagca gaaaagccaa
      181 tgaaattggt acagcataag gcacaccgtt ttcacgaatt gattttcgaa aaaaaagaag
      241 acctgccaga acaaccagag cgccgcaaaa ggtcattgcg acgagaaaat tagccagttc
      301 atgttcatca atagctaacg aaagaacagt taacagcttg acatcaccgc cgccaattat
      361 ccctgcggaa aaaagaaaga agcccacgag caaaacgctc ccggctgcaa ccagattggg
      421 tttttgatgc tgtaaaatga gtgccaaaaa aataagcacg gttaacacca gtaaataacg
      481 atggctaata attctgagta atatatcccc ccatgaaata aaaataagaa agagcgcaac
      541 aaataaaaga tcgactctta gtaaccatat tgctgccatt gaaagactcc atgtaccata
      601 caaggttgct gccagacata ttcctttacg tctgacagcc tctagagagt ttttttattt
      661 tttataagtt acggattact cgttccggaa atcgttacct gttgaattgc tgcagcgata
      721 gcgtcgaatg catctttaat tgcgcccagg aagcctgagt tctgatcgcc aaagatgaaa
      781 gcaagcagag ttgccatagc aacaccaatc aatgcatatt cgattgcagt aacaccacgt
      841 tcatccttcg cgaaacgcgc aaagcactgt ttagttttac catagtactt agtaataaaa
      901 gtattcatgt tttcctcgta tattattatt taaattcaaa ccaccagctt catctgcgtg
      961 tcaccggtat cccatatttt tgggatgtgg ctataattat ctattcccgc tatttagtct
     1021 tcaaaaaaaa ttacatttaa tatcatttat cttgattaag aaatagtaag actaaaaaaa
     1081 caagcgtcgt cagccagcac actgctaaca cggtaagaac gctaataaaa acactccact
     1141 gcttcgttct ataccattca atatttttgt cagtatgttt tattgggagc agctcttcag
     1201 tagagaattc ctggctgtta tcattctcag gaatgtttgc atcagtgttt acttccctgg
     1261 cctcgctatc gttggtatag ctactactgt cttcatcact gtcacgctca ttctcacaga
     1321 catgtttctc gacctttccc agatatctga cgccttttcg cggaatggtt ttaattagag
     1381 aactcgacaa acctgcgacg tctaatgcct ttctaagcat gtaaatctgc ccataataac
     1441 tgctgtcgct taccgaacct ttctgctccc gccaaacctc attgataata tgttgcttat
     1501 cggattctcc ctgtagtaaa aggagtaaaa atcgatagcc gttttcagac agattgatta
     1561 cattgcctgt actggtggtg atacgtcgag acgctgggta aaacttaact ccctgcccta
     1621 aagaaaaata aaaacggtca tcgctcataa gtaccgaccc ctgttttctt cttcacatga
     1681 ctactcatta caactttgta tgaagtaaga tgcaaaatca tattgtttac attaagttaa
     1741 g
//
LOCUS       DAJTUI010000252         1726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTUI010000252 DAJTUI010000000
VERSION     DAJTUI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.256"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..522)
                     /locus_tag="OO464_004211"
     CDS             complement(<1..522)
                     /locus_tag="OO464_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6229106.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWG"
     gene            complement(588..1217)
                     /locus_tag="OO464_004212"
     CDS             complement(588..1217)
                     /locus_tag="OO464_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229107.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1221..1436)
                     /locus_tag="OO464_004213"
     CDS             complement(1221..1436)
                     /locus_tag="OO464_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6229108.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          458 a          463 c          361 g          444 t
ORIGIN      
        1 cccccaacag gctaatgcgc tttcctggtc acgacgaata acctgtccat agcagttatt
       61 tgaacgtatg cggcaatcgc gcccaacatc ttttatccac cagcgaatcg cctcgcatgc
      121 gcccttacga tcaccggcat tcagccgctt ataaaacgtc gacgggaaac acttaccggg
      181 gccaatgtta taggggcaaa atgacgctat acccgctttc tggggttcgg tcagaggtac
      241 tttaatattg cgctccaccc atgccagcgc cttatcacgt tcaatagcgt taacctggtc
      301 acatttttcc ttcgacagct tcattcccgg tatgacaggc ttaccatcca ccattgtggc
      361 accacgacag atggtccata tgccagaacc atcacggtat gccgttgtgt gattaccctc
      421 tttttcgttc agaaactggt caagtatctg gggagcagac gcgcctgcgg caatcagcgc
      481 cagaacagca gccgacaggc cgtatttgat ttttgcgctc atggatattt atcaggattt
      541 atcggcaaca aataacgagc caacttatat acgcctttta agataagtca gccccggctg
      601 gatccagtca tcaggctctt tcttaaaggg ggagtattga aatcacgaag aagagcctcc
      661 cgcaccgcag catccatgtc tgcaccactg gcaagtgctt caatctctgc cgccacctgc
      721 aaatacccca tgcaacggcc aatgcgtttc ataactctgg tgttttcgtc tttcaggcat
      781 ctgatagcga gattaataat tttcaggcag tgatgatgcg atgcccggac aggttttccg
      841 ttctcataaa cgacgaactc accctcttca caggaaaccc tgaaaggccg atggggttcc
      901 tggtctttat ccggtgcaga cgcaataaaa tcacttttat gctcttcctc tccggcactg
      961 ttaaccgtaa tgacgtactc aggccgcagg taagccgcct ctttttctga caatgcggcc
     1021 ggtaaaaact tttcctgcaa aaacttcata aaagtctcgt gagcgacttt gtggcagtaa
     1081 tcgtaaatcg ccgcataatg ctcatcgcgg cgtttgtttt catcttcaga actcaccaac
     1141 gccgacaatt ttttattaag ctcagtgatt tcattttcca ggtgattgaa tcgctgattc
     1201 atttcttcat gcttcattat ttactctccc cgtgcagcct tacgcttgtc ttctcttatt
     1261 ttgaaataca gattcgtcag ataagtcaga agccccagaa gcagacttcc cagcacacca
     1321 atcgcagccc actgtgatgg cctgacctga tccaaccact gcaaaaacca gtagccggca
     1381 ctgccggcgg aggtgccgta ggcaatgccc gttgaaattt tgtccatgga tttcatagcc
     1441 tcacctccgc aaataacgga tggcgtagtt ttacactgag aaatgaaagg gatttgaaaa
     1501 gaaaaaaacg caaaagcggg cgaaacgata tatacagtaa ggaaagcact ctatccaaca
     1561 aaccacccac agttaatcgg aataaaagca gagtgcttat gaatgatcgc acgcccgaag
     1621 gttagtattt ctgcacagca attttgcaaa aaaaggcgat cattcataac ttaaacgtct
     1681 ttcagtcact ccgggatttc ccatcatcgc agactgaaag actctg
//
LOCUS       DAJTUI010000253         1714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTUI010000253 DAJTUI010000000
VERSION     DAJTUI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.257"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO464_004214"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO464_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR6229109.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="OO464_004215"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="OO464_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1714)
                     /locus_tag="OO464_004216"
                     /pseudo
     CDS             complement(1292..1714)
                     /locus_tag="OO464_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          509 a          302 c          383 g          520 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttag ttgtaattta
      901 tttatggagg ttttctatgg aattcttttt ttattgtggc tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aagccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgaca aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt caggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatc
//
LOCUS       DAJTUI010000254         1673 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTUI010000254 DAJTUI010000000
VERSION     DAJTUI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.258"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..88
                     /locus_tag="OO464_004217"
     CDS             <1..88
                     /locus_tag="OO464_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6229110.1"
                     /translation="EKPETIQPQSRPSSAITVSKTGYVFFDR"
     gene            196..>1673
                     /locus_tag="OO464_004218"
     CDS             196..>1673
                     /locus_tag="OO464_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR6229111.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIG"
BASE COUNT          443 a          357 c          479 g          394 t
ORIGIN      
        1 cgagaagcca gaaaccattc aaccacaatc cagaccatca tccgcaatca ccgtatcgaa
       61 aactggctat gtgtttttcg acaggtgaac catgaaatca aaaatcaaat cgctactggt
      121 cgctggttat aaccacggct ggttaagtat ttcgtttgtc gatttctggt ttaaaaatct
      181 caatctgagg gaatcatgag gccaagtgaa cttagcgacc tgctttgggc gcaggttgac
      241 agggtggctc cgcacctgtt gccaaacggc aagatagagg ggcatgagtg ggttgccggt
      301 aacgtcaacg gtgacaaggg aaacagcctt aaggtcaacc ttatcggcaa gaaaaaatgg
      361 gctgatttcg ctgagggaga cggcggtgac atgcttgatt tgtggatggc atgtcgtgga
      421 attaacctgc atcaggctat gcaggaagcg aaagcatttc tcggtatcaa ggatgacgat
      481 caccatttcg atgccagacg tgagaagaaa ttctccagac ctgatcgtaa gaaaatcgcc
      541 cgctacgtta ccagaacaga atcccatctt gagtacctgc aatcgcgtgg catatcgcca
      601 gaagtcgtaa agcgctacga ggttgtcagc ggcaaggtgt ggaatggaga acgagaactg
      661 gatgctctgg tgcttccgta caaacgcgat ggtgagttgt tgcaggtcaa gcgaatcagc
      721 actgagcgcc cggacgggaa gaaagtcatt atggcagaag gtgattgcga accttgcctg
      781 ttcggatggc aggctctgga cgctggcgtg agggtggttg tgctttgcga aggcgaaatt
      841 gattgtatga gctatgcgca atacggcatc tcggcgttat ccgtgccgtt tggtggcggg
      901 aaaggcgcta agcaacagtg gattgagttt gagtatcaca acctcgacag gtttgaggaa
      961 atattcatct cgatggacgt tgatgatgtt ggtcgtgaag ccgcaaggga aatcgtaagc
     1021 cgactcggtg aacatcgttg ccgtcttgtt actctgccgt acaaagacat caacgaatgc
     1081 ctgatgaacg gtgttaccga ggatgaaatc tggcagtaca tcggcacggc atcctacttc
     1141 gatcctgaag aactctacag tgcgcgagag ttttaccagg acactatcaa cgctttctac
     1201 ggcaagcagc agtatctgtt taatccaccg tgggaatctc tggcagataa attccagttc
     1261 cgtgaggcag agttgacgct ggtcaatggt gtgcacggtc acggaaaggc atgcccactg
     1321 aatgagccta ttcttttagc tgatgggaca tggactactc acgggaatgt aaaaattggc
     1381 gatcaggtgg cgtcagtaga cggcaatccg tcaactgtca ctgggatatt cccgcagggt
     1441 gttagagatg tttaccgagt cacatttgaa gatggtcgtt atgttgattg cgcaggcgat
     1501 cacctatggg aggtcactag tcgtggattc acgaaaggcg agaaacgccg cgtgatagat
     1561 accttcgggt tgaagcggtt gagtgaaacg aagaggcaca aaaatggcgt taggattcct
     1621 gaaataactg gtgactttgg cgaccactca gagccattag catgggttat cgg
//
LOCUS       DAJTUI010000255         1604 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTUI010000255 DAJTUI010000000
VERSION     DAJTUI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.259"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            240..509
                     /locus_tag="OO464_004219"
                     /pseudo
     CDS             240..509
                     /locus_tag="OO464_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..732
                     /locus_tag="OO464_004220"
                     /pseudo
     CDS             506..732
                     /locus_tag="OO464_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="OO464_004221"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="OO464_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR6229112.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          477 a          330 c          377 g          420 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaa
//
LOCUS       DAJTUI010000256         1577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTUI010000256 DAJTUI010000000
VERSION     DAJTUI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.260"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..626)
                     /locus_tag="OO464_004222"
     CDS             complement(<1..626)
                     /locus_tag="OO464_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6229113.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFIN"
     gene            complement(740..1312)
                     /locus_tag="OO464_004223"
     CDS             complement(740..1312)
                     /locus_tag="OO464_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCR6229114.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          471 a          330 c          312 g          464 t
ORIGIN      
        1 tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa aggtaacgtg
       61 agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac gatgacatcc
      121 atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc ttcccggcgc
      181 catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg tcgccagaac
      241 agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt gctcgctgat
      301 ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg caatgacaca
      361 cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg tgatactggc
      421 ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc tacccaagcc
      481 tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt atcagcatta
      541 ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt taatgctggt
      601 tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac tgttgtttta
      661 aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa gccaactgaa
      721 taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa tcagcgttcg
      781 ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac ttcatttcag
      841 ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta acctgcgaat
      901 tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct tgtcctgtac
      961 cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg tcgccgtcgc
     1021 cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt gccgggcaat
     1081 tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct gaaaatgcat
     1141 ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta acttcacaag
     1201 tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt gcatgcccgg
     1261 ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc ataaaaactc
     1321 cattgatata atatatcctt taccatgaat catcacttat attaataagt gtgtttttga
     1381 ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta attacgcaaa
     1441 aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca agtttgttat
     1501 cagacattaa tgataattca gaatatattt atatagaaag atagatgaat ttattgatgt
     1561 cagttataaa aaagggc
//
LOCUS       DAJTUI010000257         1532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTUI010000257 DAJTUI010000000
VERSION     DAJTUI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.261"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..291
                     /locus_tag="OO464_004224"
                     /pseudo
     CDS             1..291
                     /locus_tag="OO464_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            293..659
                     /locus_tag="OO464_004225"
                     /pseudo
     CDS             293..659
                     /locus_tag="OO464_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            587..1060
                     /locus_tag="OO464_004226"
                     /pseudo
     CDS             587..1060
                     /locus_tag="OO464_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
BASE COUNT          380 a          380 c          460 g          312 t
ORIGIN      
        1 tcaacgtatt accactgtca gcaacagcga catcatcatg ataaacgcag tgcccgtgcg
       61 cagcgcgatg actggctgaa gaaagagata ctgcgcgtat acgatgagaa tcatcaggta
      121 tacgctgtgc gtaaagtctg gcatcagttg ttacgggaag gtatcagagt ggccagatgc
      181 actgtggcac gtctcatggc ggttatgggg cttgccggtg ttctccgggg taaaaaggtc
      241 cacactaccg tcagccggaa agccgttgcc gcaggcgacc gcgtaaaccg tcatcaaggc
      301 aatgtgccgc gtactccggg tggcccgcag cggctggtat acgtggtgtc agcggcggac
      361 aaggataaac acacgtccgc agttccgtca gcactgcgac agcgttgtcc gcaaggcttt
      421 tacccagtcc agcgttacgg tgccccacgc ctgacggatg aactgtgtgc tctggttacc
      481 actttaacgt aaaaaccgtg gcagccagtc tgcgccgtca ggaactgagc gcaaaagcct
      541 cccagaagtt cagcccgatc agttaccgtg cacacggcct gcctgtatca gaaaatctgc
      601 tgacgcagga tttttacgcc agtggcccga accagaagtg ggcgggagac atcacgtact
      661 attacagctc accgactgcg gggaaacacg gggcgccggg ttattgaagg tgacctgtcc
      721 agctacttcg acacagtaca tcaccggctg ctgatgaaag ccgtctgccg caggatcagt
      781 gatacacgtt ttatgagact gctgtggaaa acgccatgtt gatgttggtc tcttccgggc
      841 ggtcagtgaa agtatgccac agggcggtgt gatctcgccg ttgctgtcga acatcatact
      901 gaatgagttc gaccagtatc tgaataaacg ctacctgagt ggaaaagcca gaaaggatcg
      961 ctggtactgg aatcacagta tccagcgagg ccgaagtact gctgtcaaag aaaactggca
     1021 atggaaacct gccgttgctt actgttgcta tgccgatgac tgtgtgccac gaaggcgcgt
     1081 cctggggacg tgagtccctg tgcagccatg cagcacaaga gatgagggaa ggccccttgg
     1141 tgtcggcgtg caggcgcagg caccaaacca tccactgctg ctgcatttaa gagatgtggt
     1201 ggtgagcgag atgggaaaag gtcggacgtg agccgatcag gtatggttcg aagagacgaa
     1261 cgaaagtgaa ccgctgatga tgtgtcgaaa gcatatagat gatgtcaaaa caatggtgga
     1321 gtcgttcctg tgggatatgt ttactgcctg cgcggcatcc ggcataaaag cggcgtgaag
     1381 tcgattcagg ctcttgtggg gaacgtggac ctgtcacccc gatgtgaagg gagaagcgca
     1441 agtggaagcc ccatgagcgc cagagtacca atgcggggta cagggacgga gcaccaagcg
     1501 attgcgcagt aaacatgctt gacaacttaa tt
//
LOCUS       DAJTUI010000258         1474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTUI010000258 DAJTUI010000000
VERSION     DAJTUI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.262"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(5..184)
                     /locus_tag="OO464_004227"
                     /pseudo
     CDS             complement(5..184)
                     /locus_tag="OO464_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            708..992
                     /locus_tag="OO464_004228"
     CDS             708..992
                     /locus_tag="OO464_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR6229115.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            992..1267
                     /locus_tag="OO464_004229"
     CDS             992..1267
                     /locus_tag="OO464_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6229116.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          402 a          368 c          361 g          343 t
ORIGIN      
        1 aaaattactc tttttcgaac tccagtgagc gttcacgact catcagtgtg aaaatgatgg
       61 caaaaacagc agacagtatc agtacgataa atgtggcccc ccatctcaga taatcgacaa
      121 caagtcccat tcccagtgtc gcaacaattg agccgaagat ataggaaaat gtgcctgaca
      181 gccctaacag aataagttta cgcgtaaatc gccctgaatc cagaagagca ttaagtatca
      241 cataaacagg ttccatacca gtcctgaccg catcaggacg ccagtaaata aaatctcgta
      301 gtccaccggc atcagagaca agagtaatcg tcgttaccgg tatttcgact ggtttgtact
      361 ggtgtgagcg gacaaaacta ctgtacgtct ctcatacacg ctcgtaagcg gccctcacgg
      421 cccgctaacg cggagatacg ccccgactgc gggtaaaccc ttgtcgggac cactccgacc
      481 gcgcacagag gcattttcat cgctgacagg ccgcatggcc tcgcagagcc ggagggggac
      541 tggtgatatt cggacgaccg taccggcttg cgccgggctt cggcggtttt acacctgtgc
      601 tacatgtaac caccgtatgc cgccttatat gcgctgactc gcattatttg ccttgttccg
      661 tctgaaagta atcactatga ttaaatatga ttaacaacta atcgggtatg caaatgaaaa
      721 acaataccgc acaagcaaca aaagtaatta ccgcgcatgt gccattacct atggctgata
      781 aagtcgacca gatggccgcc agactggaac gctcccgggg ctgggttatc aaacaggcgc
      841 tttctgcatg gcttgcccaa gaggaggagc gtaatcgcct gacgctggaa gccctggacg
      901 atgtgacatc cggacaggtt atcgaccatc aggctgtaca gtcctgggcg gacagcctca
      961 gtactgacca tctgttaccg gtgccacgct gatggaactg aagtggacca gtaaggcact
     1021 ttctgatttg gcgcggttat atgattttct ggtgctggcc agtaaacctg ctgccgccag
     1081 aacggtgcag tccctgacac aggcgccggt cattctgtta actcatccac gtatgggaga
     1141 acagttgttt cagtttgaac ccagggaggt cagacggatt tttactggcg agtacgaaat
     1201 ccgttacgaa cttactggcc agactattta tgtattgcgc ctgtggcaca cacgagagaa
     1261 caggtagatt gggtgtggag gcaactttaa catagcctgc aggtattaat tctggagcat
     1321 tgttatcagg ccagatattt tgcattccat taaataatct tgtaggagat aaaataaatt
     1381 acgatgaaat aacgaaaatt accgctcgtg actggcggca atatcgcgct ccgggctggc
     1441 aaataaccca tcagaaacgt tattgccaga catt
//
LOCUS       DAJTUI010000259         1427 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTUI010000259 DAJTUI010000000
VERSION     DAJTUI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.263"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(1..264)
                     /locus_tag="OO464_004230"
                     /pseudo
     CDS             complement(1..264)
                     /locus_tag="OO464_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(379..1017)
                     /gene="ospI"
                     /locus_tag="OO464_004231"
     CDS             complement(379..1017)
                     /gene="ospI"
                     /locus_tag="OO464_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR6229117.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1176..1427)
                     /locus_tag="OO464_004232"
                     /pseudo
     CDS             complement(1176..1427)
                     /locus_tag="OO464_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          410 a          353 c          245 g          419 t
ORIGIN      
        1 cagcaagcgc tgtaactctt ttatttgttt tatagcggca gccagttctg aggcaggaac
       61 gacatcctct ccggcagcaa cagcagtgag acttccttcc agatattatt ttttccactt
      121 aagcaacagg ctgggctgaa taccatgcaa acgcgccaca tgggacaccg tcatgccagg
      181 ctccattgtc tgctgaataa tggcaatttt ttcctgagga gttttacgtt tacgaagctc
      241 cagccccagc aaagttccag tcatctcaaa attagaacta gtgttagaca tatatccaag
      301 cctatctctt attttaagat acatccactg tctggcgttt caaggggccg gctcaaatct
      361 atgtacaggc tctcagcatc agcaaagcct cttacttttc caactactac tgttagcata
      421 gtgatcaaat tcctgatcag atacatgaat tacctgaacc gaactatttt catctacaaa
      481 tgcatctggc gatttttcta caggatcata aagattaaat ccctttcctg tttgaacatc
      541 caaaaacata attttttcac catcgttaaa tccagctatc caatgagtgg cctgttcaat
      601 agaaatcatt actacacttc cttttgaagt attttgtaaa atattttctg caatacgctc
      661 acgaaattta ctataagttt cattaggatg tgacactatg ccttcactat caatttgctc
      721 taaatgccta tgaaaaaatc cggtcatata ctctgatatt tcattaggta cgggagcctc
      781 tcttactcca taccctaaca tagccataca agcatgaagc gcacaccctg aacagttacc
      841 atccccacca gacggattta tcattttctg taaagtcgct gccaattcag aatctttatc
      901 catatcacca agacttttag agtttaaaga ttccagcaat tttttaacat ttacatttcc
      961 ctcctctgta tgtgcctcat atccagcagt tccctgtaac gacaccccat taatcatatt
     1021 gtatcctgac agtatatata ctcactatat tttcttcata acaaatatcc ctctgatatg
     1081 acaacaaaaa acggagccgg attcctgttt ttgtgaactg cagctattcg tgtctaaaaa
     1141 gtgatccact taccaccaac ggcctaatat atacagaacg gagtaaacag gactaacagt
     1201 tcccgacagg tctcatcatt ccgtggcccg aaagtgtaag ccaacacacc gccagtttta
     1261 gtgttgtacg aataccagag ccagtgttgt tgcagccttg ctgccaacga agcgccactg
     1321 ctcatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag atgttatccg
     1381 ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgat
//
LOCUS       DAJTUI010000260         1394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTUI010000260 DAJTUI010000000
VERSION     DAJTUI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.264"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..157
                     /locus_tag="OO464_004233"
     CDS             <1..157
                     /locus_tag="OO464_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR6229118.1"
                     /translation="STPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRMKER
                     RKEWGTL"
     gene            142..780
                     /locus_tag="OO464_004234"
     CDS             142..780
                     /locus_tag="OO464_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR6229119.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1008..>1394
                     /locus_tag="OO464_004235"
     CDS             1008..>1394
                     /locus_tag="OO464_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229120.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          406 a          253 c          345 g          390 t
ORIGIN      
        1 cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga atgatttctg
       61 gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt acctgcagcg
      121 tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact tggcaacatg
      181 attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc tctgaatcat
      241 ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga ctgtgtgttg
      301 tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat ggcactaggt
      361 gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc ggtggtggtg
      421 tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt actggggcgg
      481 gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat taatccggag
      541 agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc cagggggaaa
      601 caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg ctggacgaat
      661 gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct gaaacagatt
      721 agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac ggttcgttga
      781 tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa aaatgatacc
      841 gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta ggctttgata
      901 acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg cagatttaca
      961 aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg aaaaaacaga
     1021 tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg aaaatattta
     1081 aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc gaaccattcc
     1141 acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata tttattaata
     1201 aattagttcc tttttctctt ttatttgaca gggatatatc tgttcttcaa agaagagata
     1261 tttgtgttgt gcgatttacg tcagaggaaa taagtgaaca taacgtttta tttgatcatg
     1321 acattgaacg attaaaaaaa atttccaaag ctcagcttat atctcccgat tatgttttga
     1381 ttgatttttc tagt
//
LOCUS       DAJTUI010000261         1377 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTUI010000261 DAJTUI010000000
VERSION     DAJTUI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.265"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..>1377
                     /locus_tag="OO464_004236"
     rRNA            <1..>1377
                     /locus_tag="OO464_004236"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          346 a          319 c          435 g          277 t
ORIGIN      
        1 cctgatggag ggggataact actggaaacg gtagctaata ccgcataacg tcgcaagacc
       61 aaagaggggg accttcgggc ctcttgccat cggatgtgcc cagatgggat tagctagtag
      121 gtggggtaac ggctcaccta ggcgacgatc cctagctggt ctgagaggat gaccagccac
      181 actggaactg agacacggtc cagactccta cgggaggcag cagtggggaa tattgcacaa
      241 tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga aggccttcgg gttgtaaagt
      301 actttcagcg gggaggaagg gagtaaagtt aatacctttg ctcattgacg ttacccgcag
      361 aagaagcacc ggctaactcc gtgccagcag ccgcggtaat acggagggtg caagcgttaa
      421 tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt aagtcagatg tgaaatcccc
      481 gggctcaacc tgggaactgc atctgatact ggcaagcttg agtctcgtag aggggggtag
      541 aattccaggt gtagcggtga aatgcgtaga gatctggagg aataccggtg gcgaaggcgg
      601 ccccctggac gaagactgac gctcaggtgc gaaagcgtgg ggagcaaaca ggattagata
      661 ccctggtagt ccacgccgta aacgatgtcg acttggaggt tgtgcccttg aggtgtggct
      721 tccggagcta acgcgttaag tcgaccgcct ggggagtacg gccgcaaggt taaaactcaa
      781 atgaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga tgcaacgcga
      841 agaaccttac ctggtcttga catccacgga agttttcaga gatgagaatg tgccttcggg
      901 aaccgtgaga caggtgctgc atggctgtcg tcagctcgtg ttgtgaaatg ttgggttaag
      961 tcccgcaacg agcgcaaccc ttatcctttg ttgccagcgg tccggccggg aactcaaagg
     1021 agactgccag tgataaactg gaggaaggtg gggatgacgt caagtcatca tggcccttac
     1081 gaccagggct acacacgtgc tacaatggcg catacaaaga gaagcgacct cgcgagagca
     1141 agcggacctc ataaagtgcg tcgtagtccg gattggagtc tgcaactcga ctccatgaag
     1201 tcggaatcgc tagtaatcgt ggatcagaat gccacggtga atacgttccc gggccttgta
     1261 cacaccgccc gtcacaccat gggagtgggt tgcaaaagaa gtaggtagct taaccttcgg
     1321 gagggcgctt accactttgt gattcatgac tggggtgaag tcgtaacaag gtaaccg
//
LOCUS       DAJTUI010000262         1366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTUI010000262 DAJTUI010000000
VERSION     DAJTUI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.266"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            400..1149
                     /locus_tag="OO464_004237"
     CDS             400..1149
                     /locus_tag="OO464_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR6229121.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          387 a          278 c          275 g          426 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagacctg
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcac
//
LOCUS       DAJTUI010000263         1324 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTUI010000263 DAJTUI010000000
VERSION     DAJTUI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.267"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            24..383
                     /locus_tag="OO464_004238"
     CDS             24..383
                     /locus_tag="OO464_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6229122.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGIKITELGCA"
     gene            380..1063
                     /locus_tag="OO464_004239"
     CDS             380..1063
                     /locus_tag="OO464_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6229123.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
BASE COUNT          361 a          270 c          367 g          326 t
ORIGIN      
        1 ctggtgcagg aggtgctgcg ctgatgcgcc atgagtttat tttaccttat ccgccgacgg
       61 tgaatactta ctggcgacgt cgtggcagca catattttgt atcaaaagcc ggtgagcgtt
      121 atcgccgtga tgtggcgcta attgttcgcc agcagcgact gaaattaaac ctgtccggaa
      181 ggctggcgat aaagattatt gcagagccac cggataagcg ccgtcgtgac ctggacaata
      241 tcctgaaggc accgctggat gcgctgacgc atgctggact acttatagac gacgagcagt
      301 ttgatgaaat caatattgtg cgcggtcagc tcgttcctgg tgggcggctg gggataaaaa
      361 tcacagaact ggggtgcgca tgaataacca gtatttacag tttgtgcgtg agcagctcat
      421 tatcgccacc gctgatttga gtggggcaac aaaaggtcag cttgaagcct ggcaagagaa
      481 tgccatgttc gatacagggc gttacaggcg taaaaaaatc cggtaccgcg atgaagtgac
      541 tggaaaaatg ataacgcggg ataatccacc aatcccggga aagcaatcgc tggcgaaggg
      601 gacgtcaatt cctctggtca gtccggttga gttttcgaca tcatcgtggc ggcgggctgt
      661 tctgtctctt gaagagcact ataaagcctg gttgttgtgg tgttacagcg ggagtatttg
      721 ttgggaatat cagatcgcga taacacagtg ggcgtggaat gaatttaatg ctcaatccgg
      781 taccagaaaa attgcaggga aaacgcagga acgcctgaaa aaattaatct ggctggcggc
      841 gcaggcagta aaagcagaac tttttggtgg ggaaggttat gaataccagg agctggcatt
      901 actggcggga gtgacaacta aaaactggtc caaaacattt actcgtcact gggttgcaat
      961 gaaacacatt tttcaccgac tggatagtga ggctttattg tttgtaatga gaacacgttc
     1021 aaaaaaaaga cggcattttc aaagcaaagt gttgcaaaag tagattgaaa ggcatatatt
     1081 catgcaaatc tgatactttg ccgattttgt acgtgatggc aaaagcaaac aaaacccgcc
     1141 cacaagcggg ttttttgtgc cacttatctc ggatagacat ggtgaatgcg ctggtggagg
     1201 agataagggt gatttttgaa tgcttgcaac attgatttcg taacgttatt atcctgcgcc
     1261 cggcccttta gctcagtggt gagagcgagc gactcataat cgccaggtcg ctggttcaaa
     1321 tcca
//
LOCUS       DAJTUI010000264         1291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTUI010000264 DAJTUI010000000
VERSION     DAJTUI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.268"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..823)
                     /gene="ipaH4.5"
                     /locus_tag="OO464_004240"
     CDS             complement(<1..823)
                     /gene="ipaH4.5"
                     /locus_tag="OO464_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR6229124.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPD"
BASE COUNT          364 a          233 c          286 g          408 t
ORIGIN      
        1 agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac aatggattcc
       61 ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt aatgaagtta
      121 gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat aatctgcgta
      181 gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt agttcctgta
      241 gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc aagacagtaa
      301 ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt aacgtctcca
      361 gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc agacatgaca
      421 aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc gggagtatat
      481 tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca ggtagggaga
      541 ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt ggtaaagaac
      601 gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat attttcaata
      661 atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag tcatcccaga
      721 tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata catgataagc
      781 cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa cccctcacta
      841 ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa acaatcctag
      901 agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc cagtaaccgt
      961 cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat ccagtttgga
     1021 ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt actgtctcga
     1081 tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt gcacgccgta
     1141 aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt aatcgggtca
     1201 cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga ttatgaatgg
     1261 tgcagtcgtg agccgttttc agacaatcgc a
//
LOCUS       DAJTUI010000265         1275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTUI010000265 DAJTUI010000000
VERSION     DAJTUI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.269"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            34..435
                     /locus_tag="OO464_004241"
     CDS             34..435
                     /locus_tag="OO464_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6229125.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="OO464_004242"
     CDS             447..818
                     /locus_tag="OO464_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6229126.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1275
                     /locus_tag="OO464_004243"
     CDS             805..>1275
                     /locus_tag="OO464_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6229127.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          303 a          286 c          396 g          290 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atccgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccg
//
LOCUS       DAJTUI010000266         1263 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTUI010000266 DAJTUI010000000
VERSION     DAJTUI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.270"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..560
                     /locus_tag="OO464_004244"
     CDS             <1..560
                     /locus_tag="OO464_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229128.1"
                     /translation="RQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQAA
                     AIVELAEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEPM
                     KSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNEI
                     GVSGGSLAALTSPLISTTPVAENVK"
     gene            799..858
                     /locus_tag="OO464_004245"
     CDS             799..858
                     /locus_tag="OO464_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6229129.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1175..1233
                     /locus_tag="OO464_004246"
                     /pseudo
     CDS             1175..1233
                     /locus_tag="OO464_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
BASE COUNT          345 a          298 c          344 g          276 t
ORIGIN      
        1 ctcgccagac cgcagaagcc gatcgtgcta aacaagttgc cctaatcgct gccgcgcagg
       61 atgcagaaac caaagcggtt gaactgaccg tgcgggcgaa agcagaaaaa gaagccgcag
      121 agatgcaggc ggcggctatc gttgagttag ccgaagctac acataaaaag ggtctggcgg
      181 aagcagaagc acaacgtgcg ctgaacgatg ctatcaacgt actttctgat gaacaaacca
      241 gccttaaatt caaactggcc ttgttgcagg cgctacctgc ggtaatagaa aaatccgttg
      301 agccgatgaa atctattgac ggtatcaaga ttattcaggt cgatggtctg aatagtggca
      361 gcgctgcggg tgatgcaaac acgggtaatg tggggggcgg aaacctagcg gaacaagcat
      421 tatcagccgc tctctcttac cgcacacagg ctccgctgat tgactccttg ctcaatgaaa
      481 ttggcgtttc aggcggttca ctggcggcat tgacttcacc cttaatctca acaactcccg
      541 tcgccgaaaa cgtaaaataa attttcagct atgaacgtcc ctggcacaaa aagccgggga
      601 ttttttatat ctgcgttccg ctaaaaactg caaatgatca ggtcgttgca acgttttgcg
      661 tgaccgctcg gggaaggcaa aactgcctct gggaaagcgt tgcgcgaggt ccggcgctca
      721 tcaacaatcg gggagtcagc aaagggctga aacgggaaag cccctcccga ggaaggggcc
      781 ttacagaagg aaagggttat gatgaagctc gtcatcatac tgattgtgtt gttactcgta
      841 agtttcgcag cttattaaca gccaatcaga ggggggagaa atcctccctc acccttgttc
      901 ctttacttta ggttgaaaaa aaatcagcgt caagaaggtt tcattaacac atatccctgg
      961 cccaaaaagc cggggatttt ttatatctgc gttccgctaa aaggtgcaaa tgctcaggac
     1021 gttgcagcgt tttgcgtgac cgctcgggga aggcaaaact gcttcaggga aagcgttgcg
     1081 cggggtccgg cgctcatcaa caatcgggga gtcagcaagg ggctgaaacg ggaaagcccc
     1141 tcccgaggaa ggggccttat agaaggaaag ggttatgatg aagttcgtca tcatactggt
     1201 tggttgttac tcgtaagttt cgcagcttat taacagccaa tcagaggggg gagaaatcct
     1261 ccc
//
LOCUS       DAJTUI010000267         1218 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTUI010000267 DAJTUI010000000
VERSION     DAJTUI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.271"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..261)
                     /locus_tag="OO464_004247"
     CDS             complement(<1..261)
                     /locus_tag="OO464_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6229130.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMING"
     gene            complement(258..548)
                     /locus_tag="OO464_004248"
     CDS             complement(258..548)
                     /locus_tag="OO464_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6229131.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(548..1147)
                     /locus_tag="OO464_004249"
     CDS             complement(548..1147)
                     /locus_tag="OO464_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6229132.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          281 a          327 c          275 g          335 t
ORIGIN      
        1 gccattgatc atctctcgca aaaacgcttc cagctcaggt ttctctattc ccgctttttt
       61 catcctgcgc agggcttcat tgacggctgt tttcgtcaat tttttggatg ccagcaactg
      121 gttaaacata tttcccgtct taccgccgcc aatatacgac cagcgccccc acatgcgcag
      181 ttttccctga atccagacac tttccagcgt ggtgagacga aggtgttccc cgcttttgcc
      241 tgtatttgtt gggtaaatca taaataacct tcctttctcc agatttcttg tgtgcgaaaa
      301 acaccttctg catgcatcag gcgtaattct tctttggtgt aatcgctggt ttttacccgc
      361 ccgtcgatta aatcgtggca tgagctacag gcaatcgctg cctgcatatc gtgtggtttt
      421 gtcgctgttc cgcacgtccc cgccagcctg taatgcgcca gcacagaagt ttcgggattg
      481 tgattgcagt agccaggaat tctgacggtg cacatctgcc cccgcgccgc tttacgtaaa
      541 tccaccatta cgcaaactcc agtaactgcg cggccacatt ttcgacttcc tccggagagg
      601 aaaatttacg gaacaggatc cagttccaca gcacattcag tacagattta taaacctgct
      661 gaaactcggt ttcgtccatg ttcgcaaatg cgatagattt tgccctgcgc ccacgactac
      721 catcaggata aatatgctcg gtgtaaaatc cggcctgaat ggttacccac tcgcggaaag
      781 cgtcaaacga ctttagcaat gccgtatccc gggttctgca tgtcgcaact gtattaaggt
      841 attgctctgc ggcatcactc agggctggag tgtgttcccg acctactgat tcgcacaggt
      901 aatcaacgaa gcctgatacc agttttcgtt cgcgaggcgt gatcgcccca ccgaccggag
      961 tccagtaatc aaatcccagt tgcaggagtt tgaaaaaacg cttgtggaat gcgtagttac
     1021 gcacacgctt aaagtctgcg tgtatccact cacctatttt gatttgatgc aaaaaatcgc
     1081 aactctccgg cgtcgccggg agaagtaatc cggaagaggt ttgtttgacc agttgtatat
     1141 gcgccatcgt agttctccgc tggcgcagta gaatgggtgt tcagcccgtt atgtagtata
     1201 ccagaattaa tgccaata
//
LOCUS       DAJTUI010000268         1217 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTUI010000268 DAJTUI010000000
VERSION     DAJTUI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.272"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            623..>1217
                     /locus_tag="OO464_004250"
     CDS             623..>1217
                     /locus_tag="OO464_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6229133.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICAL"
BASE COUNT          332 a          265 c          285 g          335 t
ORIGIN      
        1 ggcttcaccc gtgttaatat cctggataac tgtgtttgaa ggatttctgt taataatctg
       61 ctcctgcaac tcacctgatg agtattagtg gtaatacata atcttactcg taaaagagta
      121 taccttatgt cattaaacag tcttccggcg tgttgttcaa tcctgtacct ctgttactta
      181 tctggtacgt tgatatgtgt aatgctacgt taattgcaat atctggttat ggctttatac
      241 cttcagcatt taaaatattt tattaccgga gaaacaggga agtggaaaga ctttcgggat
      301 aatgtcaggc cattctgacg ttaatcacag taccaaataa cgagagggta atggaagtta
      361 aaacctgcgt aagttcctct gttgttcact ggaaaaagtg attgcccaca cgcgttataa
      421 aggcctttcc gctgcagcct ggttaacaga gcttcaggcg ttatatgcgc tgtatggctg
      481 ttacgtatgc tgttctttgc ctggttctga tacccgtgga agtcgttacc ggaggaaaca
      541 cgttcaccct actacctgag aacatcttct tcaaaaatta cggcaacgct tcttcctgtg
      601 ttacagaaca cgaggaagaa acatgcaaac ttataacacc aatcccgctt acgaaatggc
      661 tccccagctc ctggagcact ttaaccagca tctggattca ttattcgggg tctattcgaa
      721 actgttaccg ttcagaatgg attttgctta ccgaaaaaac accctcagct acagatgtgc
      781 ctgcaggtac gccatgtgcg cggagatgct gcaactgatt aacgaggtgg gagagaaact
      841 ggtcgggtat gcctgggtga tggaatatac agaacgtaaa gggcttcaca ttcattttgt
      901 gggctatctg aacggccaga gccacaggag ttcgtatctg gtatccagat tgatgggaga
      961 tatctggagg cgggttaccg agggtaatgg ttactaccac tggtgccggt ttaataaaaa
     1021 ttatccggta aacattaacc acgtcattca ctattcagat cacaaagcgg tgaatgattt
     1081 acgctatgcc atcagctacc tggccaaacg ggaacagaaa gaatgtggca tcatcctgaa
     1141 atgcagcggg cttcctgaaa aaagtaaccg gggtcgacca cgactggaca gccccctgcc
     1201 ggggatatgt gctctgg
//
LOCUS       DAJTUI010000269         1187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTUI010000269 DAJTUI010000000
VERSION     DAJTUI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.273"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            42..>1187
                     /locus_tag="OO464_004251"
     CDS             join(42..279,279..>1187)
                     /locus_tag="OO464_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6229134.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNS
                     CCWPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIH
                     LDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPE
                     KQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT          318 a          281 c          318 g          270 t
ORIGIN      
        1 acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt aagcgttatc
       61 ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat tctgttgcca
      121 gcgttgcaac acgtctcgat atcaccaccc acagccttta tgcctggata aagaagtacg
      181 gtccggattc ttccactaat aaagaacagt cagatgctca ggccgagatc cgccgtctcc
      241 agaaagagct gaaacgggtt accgacgaac gggacatatt aaaaaaagcc gcggcgtact
      301 tcgcaaagct gtccgactga ggtacgcctt tatccgtgac aactcctgtt gctggcctgt
      361 tcgcctgctc tgtcgggtgc tggatgttca tcccagtggt ttttacgcct ggcttcagca
      421 gccgcattca caacgccatc aggcagacct gagactgaca ggacagatta aacagttctg
      481 gctggaatcg ggatgcgtct atggttatcg caaaatccat ctggatctgc gtgacagcgg
      541 gcaacagtgc ggagtaaaca gagtctggag actgatgaaa cgtgtcggaa taaaggctca
      601 ggtcggatac cgaagcccgc gggcacgtaa aggcgaggcc agtatcgtgt cacccaacag
      661 gctccagcga cagttcaatc cggatgctcc tgatgagcgt tgggtaacgg acataaccta
      721 catcaggacc cacgaaggct ggctgtatct tgccgttgtt gttgatctgt tctcacgcaa
      781 aattatcggc tggtccatgc aatcccggat gacaaaggac attgtcctga acgcactgct
      841 gatggctgta tggcggcgta atcccgaaaa acaggtgctg gttcattcgg atcagggcag
      901 tcagtacaca agccatgagt ggcagtcgtt cctgaaatca cacggcctgg agggtagcat
      961 gagccgtcgc ggtaactgcc atgataatgc ggttgcagaa agttttttcc agttgttgaa
     1021 acgtgaacgg ataaagaaaa agatctacgg aacgcgggaa gaagcccgca gtgatatttt
     1081 tgattacatc gaaatgtttt ataacagtaa gcgtcggcat ggttctagcg aacagatgtc
     1141 accgacagaa tatgaaaacc agtattatca acggctcgga agtgtct
//
LOCUS       DAJTUI010000270         1185 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTUI010000270 DAJTUI010000000
VERSION     DAJTUI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.274"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(333..479)
                     /locus_tag="OO464_004252"
                     /pseudo
     CDS             complement(333..479)
                     /locus_tag="OO464_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            533..715
                     /locus_tag="OO464_004253"
     CDS             533..715
                     /locus_tag="OO464_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCR6229135.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(816..1145)
                     /locus_tag="OO464_004254"
     CDS             complement(816..1145)
                     /locus_tag="OO464_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR6229136.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
BASE COUNT          326 a          264 c          291 g          304 t
ORIGIN      
        1 agcaatgtct gtcgcacaac aaccacaatg tcacaaataa aaaatgagaa taaccagttc
       61 cctgaaggcg acgtagcccg gtacaagagt aacccacgtt agcgcttggc gacggcggaa
      121 caaagctgat tcgcgacgta agaaagtggc aggctcatat gacggacctc tgtaatgcgg
      181 tatccaaccc gcgaatatca gcatgattca ccgtcggatt tactgctacg tcgccctcag
      241 ggaattaaac ctaaaaagaa aatcgtcaaa attagagatt ccggtttgac acggaaagtc
      301 atatcagcgt caacggagca ccgtattgac gcttatttat tggtgagaac tacgttccat
      361 ggcaggagtt cgtcaacacg gttggagggc cattcaggca gtatgctcag aatatggcgc
      421 agatacgctt ccggatcgat accgttcaga cggcaggtgc ctgcttttcc ggcaacaagc
      481 aggaatctca gcgctgaagc aggaattaga ggtaaaaacg ccctaccggg ccatgaatca
      541 cccggtcatt ggagtagtaa ccaaagcaga tttagccagc atggaacaaa tatctttggt
      601 gaaaagttgg ttacgggaag ctggagcgca caacgtgtta gtaaccagtg cagttaataa
      661 caatggagtt acagaacttt ttgccttgct gcatacagaa gaaggctgtt gttaatttcg
      721 attggttctc agatgagaat gatgacggtg atacacagat aagaatcaat gagcaatgag
      781 ggttgccggg caaccctcat tgaataaaac gggaattact tcgcttcgcc gtttttcatt
      841 tcgcccatag ctttcagctt tttggagata tcgcggcgtt ctttggagag ctcggcattt
      901 ttgatgatgt aatcgtcaac gcgatcttca tagtcacctt tcatgctggc gatgatgccc
      961 tggattgctt caacgctcat ccctggcttg atgtaatcgc tcaggttgtc cagcaacagg
     1021 acgcgcttct ggttgtcacg gatctttttc tcaacgtcct gaatttcacg ttgcagtttg
     1081 ttcttacgac ggaacagacg aacaaattcc agtacgtcct ggaatgaagg cttggtagtt
     1141 tccattttta tacccctgat aatgtgagag tcggattcgt ttaag
//
LOCUS       DAJTUI010000271         1182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTUI010000271 DAJTUI010000000
VERSION     DAJTUI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.275"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..148
                     /locus_tag="OO464_004255"
     CDS             <1..148
                     /locus_tag="OO464_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6229137.1"
                     /translation="CIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFEN
                     QNLA"
     gene            487..848
                     /locus_tag="OO464_004256"
                     /pseudo
     CDS             487..848
                     /locus_tag="OO464_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            935..>1182
                     /locus_tag="OO464_004257"
     CDS             935..>1182
                     /locus_tag="OO464_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6229138.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
BASE COUNT          319 a          276 c          274 g          313 t
ORIGIN      
        1 atgtatccat ggagaacact ttatcagccg ggaaataatg cgggcaacgg tgtttaatta
       61 tatcgaatgt gattacaatc ggtggcggcg gcacagttgg tgtggcggcc tcagtccgga
      121 acaatttgaa aaccagaacc tcgcttaggc ctgtgtccat attacgtggg taggatcact
      181 tacgccctgt ggttgaaacg tgagcatatt gttttcagat gctgttgtct tgcttcagag
      241 tgatctgtcc tccggattta cgcaaagtta tccacaggca ctgtgcattt aaaaaactta
      301 tcttttcttc ttgattttaa aaactcagat cgtcatatca ttatgataaa cataataaaa
      361 aactctaaac atgagaaggt ttttaacgta aggtgagagg atttttaagt gtatccatag
      421 attaaaaatg acaatgcctc caattagttg aattaggggt tataataccc gcttttaggt
      481 tctttcatgg tcacagttaa ctttcattgc ccccgttgcc agtccgttca ggtttaccgt
      541 cacgggcaga acccaaaagg acatgatcga ttccgttgcc gtgactgtca ccgggtgttt
      601 cagctcactt actgttacga ggcccgtaaa ccgggagtta aaaaccaaat cactgaaatg
      661 gctttcaacg gtgccggagt tcgcgatact ggccgtacgc tgaaaatcgg catcaatacc
      721 gtcatacgga cattaaaaag ctcccgcccc ggcggataac atctgcgcca gtcgctcacg
      781 ctgatgttgc ccttatctgt gaacttgatg ggcagtggag cttcgttggc agcaaggcgc
      841 aacaacactg tcacgaacgg tgcaatagtg atccacaccc aacgcctgaa atcagatcca
      901 gggggtaatc tgctctcctg attcaggaga gcttatggtc acttttgaga cagttatgga
      961 aattaaaatc ctgcacaagc agggaatgag tagccgggcg attgccagag aactggggct
     1021 ctcccgcaat accgttaaac gttatttgca ggcaaaatct gagccgccaa aatatacgcc
     1081 gcgacctgct gttgcttcac tcctggatga ataccgggat tatattcgtc aacgcatcgc
     1141 cgatgctcat ccttacaaaa tcccggcaac ggtaatcgct cg
//
LOCUS       DAJTUI010000272         1100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTUI010000272 DAJTUI010000000
VERSION     DAJTUI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.276"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            55..>1100
                     /locus_tag="OO464_004258"
     CDS             55..>1100
                     /locus_tag="OO464_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6229139.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          344 a          255 c          178 g          323 t
ORIGIN      
        1 cattaggaga gatacattaa atacaattta ttcacaatct aattccatat ccatatgcga
       61 gaaattaata tgctcagaaa catttcatcc tgtttatttc cacatatcag cacaattaca
      121 tcccccaacc attatttgtc cgaatgggat gattgggaga aacaggggtt accggaagaa
      181 cagcgtactg aggcggtaag aagacttcgt gcatgtctta cctctaaggg gcataaactg
      241 gacctgcgag ccttggcgct ttcctcgcta cctgtactcc ctgcttgcat taaaaagctt
      301 gatgtgagct gtaataaatt aaccatcctt actgatttac ctgaaaatat taaagaactt
      361 attgcaagag ataatttctt aacacatata tctgcattac cacattatct aataactttg
      421 gatgtgtccg aaaatcaatt agagaatctg ccgttattac cagacaccat caaatcacta
      481 agcgcagagt ataataggtt atccacactg ccttcattac ccttgaattt aaaaaaactt
      541 gaggttagga acaacgaact gcaaactctt ccatctctgc cttctaatct taagatactt
      601 aaggttgcgc acaaccatct tactgaactg ccccctttac ctaggagact gcaacttctt
      661 tttgcatata gcaatagatt aagcaactta ccaaacatcc aagaaaatat tatcatgaga
      721 agattttttt attttgaaaa caaccaaata actacaatcc cgacaaatct ttttcgttta
      781 gatcctcata taactattga gattgcaaat aaccccttat cagatcaaac tctgctattc
      841 ttaatacagc aaacttcggt tccaaatttt aacgggcctc agtttcgtat ttccctgtca
      901 gaccaaaaca gactgttttt acgccagatg ttgccgcaaa atttacattc gcgccatatc
      961 agagtcatca ctgaaggggg gcagaacttt cagatccccc ctcttcccga aactgtggca
     1021 gcctggtttc ctgaagcaga tcgtcgggag gtttctacac aatggacttc tttttccacc
     1081 gaggagaatt cccgggcatt
//
LOCUS       DAJTUI010000273         1091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTUI010000273 DAJTUI010000000
VERSION     DAJTUI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.277"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO464_004259"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO464_004259"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR6229140.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(986..1062)
                     /locus_tag="OO464_004260"
     tRNA            complement(986..1062)
                     /locus_tag="OO464_004260"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1026..1028),aa:Asp,seq:gtc)
BASE COUNT          285 a          255 c          248 g          303 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     1021 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
     1081 caccggtttt a
//
LOCUS       DAJTUI010000274         1090 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTUI010000274 DAJTUI010000000
VERSION     DAJTUI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.278"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..144
                     /gene="ospE1"
                     /locus_tag="OO464_004261"
     CDS             <1..144
                     /gene="ospE1"
                     /locus_tag="OO464_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HCR6229141.1"
                     /translation="KAAIYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRY
                     SDF"
     gene            534..>1090
                     /locus_tag="OO464_004262"
     CDS             534..>1090
                     /locus_tag="OO464_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6229142.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          396 a          209 c          181 g          304 t
ORIGIN      
        1 aaagcagcta tatacgaatt acaaatacct gctgccagcc ggacgaaaac gttgaaattc
       61 actgctacag aaatgcaatg gttgacaaag ataaatgaag ccggaattga tgaaaaacaa
      121 agccaacggt attctgattt ctgaagttaa tttcagactc gtttaagctc cattttcaaa
      181 aaaaatatat cagggtttgt tactaataac ttctgaaaac agaaaaaaat agagcagtaa
      241 ttctatttca gagaggagta actccatcta tcctcgatac gtactgaacg ttatcgtgat
      301 atattcatca tgctttcttc accattattc gagtataggg agagacgcaa tgaccttaaa
      361 taccttttta tggctgaatt accattctca ggaataacag cagactcctt cccatagacg
      421 gcttaggggg aaagcaatac ccggagaaag ggtacaaagc aaactgtttt aaaattaata
      481 agccatattc tatttaacta gaattcttat cgttgataag aattctagtt ataatgatta
      541 aatcaaccaa tatacaggca atcggttctg gcattatgca tcaaataaac aatgtatact
      601 cgttaactcc attatcttta cctatggaac tgactccatc ttgtaatgaa ttttatttaa
      661 aaacctggag cgaatgggaa aagaacggta ccccaggcga gcaacgcaat atcgccttca
      721 ataggctgaa aatatgttta caaaatcaag aggcagaatt aaatttatct gagttagatt
      781 taaaaacatt accagattta ccgcctcaga taacaacact ggaaataaga aaaaacctat
      841 taacacatct ccctgattta ccaccaatgc ttaaggtaat acatgctcaa tttaatcaac
      901 tggaaagctt acctgcctta cccgagacgt tagaagagct taatgcgggt gataacaaga
      961 taaaagaatt accatttctt cctgaaaatc taactcattt acgggttcat aataaccgat
     1021 tgcatattct gccactattg ccaccggaac taaaattact ggtagtttct ggaaacagat
     1081 tagacagcat
//
LOCUS       DAJTUI010000275         1084 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTUI010000275 DAJTUI010000000
VERSION     DAJTUI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.279"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO464_004263"
     CDS             complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO464_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6229143.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLT"
BASE COUNT          347 a          157 c          192 g          388 t
ORIGIN      
        1 tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca
       61 aaatccacag tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca
      121 accccaaaaa aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt
      181 ttatatgttt gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta
      241 tttgacaggt tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag
      301 agcaagtgac gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag
      361 tgtctggaca ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc
      421 gcttcaatag aaagatttaa tgatttgaaa ctggtccctt gagagaacat tttactataa
      481 ctttgcgctg ctatagactt ccttaaaaag cttttcacag tatcagcaca gtgatctaaa
      541 tgtttttcac ttatgccaga agaggatgag tttgttacgc atgatatgtt tttttgtaat
      601 gcttttgtat ctttgttcgg atttattttt ccatcaacaa gatcaatatt gttctgaaca
      661 ttaatatgat ttactgcttc aggtattttc ataaaacatc ccccattatg tgcgttttta
      721 tctgcagtta tttagaataa tattatctaa cttaatattt tcttgtatgt attataacga
      781 acatatttta atatgttgta cgattttcac tctgaaatcc cctgagttta atcacacttc
      841 ctttactgaa aggaattatt gcaacagtat tatttgtttt tggatatata tcttgcgcct
      901 tgttatgtag ttaatgctca gaatatgata gataaaatag agacgcaaaa cagggcatgc
      961 tcataacatg atgataacat aaaaaattag taacaataaa tgaaagaatc atgggcgctc
     1021 aacaaaaaga gggtaatgct gccaacttac tgatttagtg tatgatggtg attttaaggt
     1081 gctt
//
LOCUS       DAJTUI010000276         1051 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTUI010000276 DAJTUI010000000
VERSION     DAJTUI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.280"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..776
                     /locus_tag="OO464_004264"
     CDS             join(<1..274,274..776)
                     /locus_tag="OO464_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCR6229144.1"
                     /translation="TPRAGRPWAEHRKIINGMFWVLCSSAPWRDLPERYGAWKTVYNR
                     FNRWSKSGVINIIFNRLLSLLDANGFIDWSATALDGSNIRALKCAAGGSKKHPDIDGD
                     NGPGRSRGGFGTKIHLATDGSGLPLNIVLSPGQAHESQFAQRLLDGIGVQRQNGSMKR
                     RGHAVLADKAYSGRALRNELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVER
                     CFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            891..>1051
                     /locus_tag="OO464_004265"
     CDS             891..>1051
                     /locus_tag="OO464_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR6229145.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCAR"
BASE COUNT          284 a          267 c          278 g          222 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt attcagggcg cgcattgcgc aacgagctga
      541 aaaataacgg tataaaggca gtaatccccc gaaagtcaaa tgagaaaatg gcatcggatg
      601 gacgtgcaca gcttgatcgt gatgcgtact gcaatcgtaa tgtcgttgaa cggtgctttg
      661 ggcggctgaa agaatatcgc cgcatcgcca cgcgttacga caaaacggcg agaaattacc
      721 tggcgatggt gaaactgggc tgcatccgac tcttttatca acgcttacgt aattaaggga
      781 cacagcctaa atagctgcgc ggaatagtag atccctttga gggaacttag cccggattgt
      841 gcgatctgat caatcgccaa atcaaaacaa atcaccaacc ggactgagca atgccgatca
      901 tagcaccaat ttcccgtgac gaacgacgcc tgatgcagaa agccatccat aaaacacacg
      961 ataaaaatta tgcccgcaga ctgactgcca tgctgatgct gcaccggggc gaccgtgtca
     1021 gcgacgttgc cagaacgctc tgctgcgccc g
//
LOCUS       DAJTUI010000277         1045 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTUI010000277 DAJTUI010000000
VERSION     DAJTUI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.281"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..145)
                     /locus_tag="OO464_004266"
     CDS             complement(<1..145)
                     /locus_tag="OO464_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HCR6229146.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGN"
     gene            complement(120..920)
                     /gene="otsB"
                     /locus_tag="OO464_004267"
     CDS             complement(120..920)
                     /gene="otsB"
                     /locus_tag="OO464_004267"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR6229147.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
BASE COUNT          230 a          303 c          246 g          266 t
ORIGIN      
        1 cattccctgt ttcaccactc cagccaaacc acagtccgcc tgcggctttc agtgccccca
       61 gtatgccaac ggcaaggcca ccggcactgg cggcgtgctc gtctggtggt gcaatccggt
      121 tagatactac gactaaacga ctcatagtca tcactcctgt tattttctct tttttgttgt
      181 aatgcggcgg ttatcatttc aagccagctc cagacatccg gcacacccgc cagtcgccat
      241 gatgcctgag ttgcacctgt gccaattttt actgacattc cgcccagtcg gttaacgact
      301 gcgaagccag attcatcggt taaatcatcg cccagaaata cgggcgttcg cccgataaaa
      361 ggagcttcct gcataaaggc tgcaatcgct tcacctttac tggtacctct cggtttgatc
      421 tcgacaacac actttccctg ctgtaacgcc atttgtggcc agatctgagt aatacgttgc
      481 gctaatgtca ttaatgcgtc ttcatgctgc ggagcctgac gataatgcag cgcaaaagcc
      541 atccctttcg cctccagctc cgcgccggga tactgagcga tgaccgtatg cagttgcacg
      601 ctaatatcac gcgcaatcgc atccggcaga tgaacgatat gtgttttacc attgatgtca
      661 cggcgctccg ccccatgcac gcccgctaac gggaagcgat aaggttttgc cagtgcgtca
      721 agctccacca ttgagcgccc tgatatcaat gccaatgcac catcacttgc ggttgccagt
      781 agctgtagtc cttgcagaat attgtcaggc acgacgacct gatcgggatg cggtttgatt
      841 tccgccagcg ttccatcaag atcaaaaaac caggcatatt tcgcggatag ttcaggggtt
      901 tcggttaacg gttctgtcac ccggctctcc tccttctctt ttcttattgt gttgcgcaga
      961 ccgcgcttta cagacatgta agtatagaca gtgtgacgga ggtcgccatt tgcaacgaag
     1021 aacagccaac cgttgtggcg gctgg
//
LOCUS       DAJTUI010000278         1020 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTUI010000278 DAJTUI010000000
VERSION     DAJTUI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.282"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..196
                     /locus_tag="OO464_004268"
                     /pseudo
     CDS             1..196
                     /locus_tag="OO464_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            342..>1020
                     /locus_tag="OO464_004269"
     CDS             342..>1020
                     /locus_tag="OO464_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6229148.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNG"
BASE COUNT          250 a          243 c          314 g          213 t
ORIGIN      
        1 tggttatcgg tggggctgtt cgccagaaaa aatttgaccc gttgtcagtt gatctgcccg
       61 actggttgtc agaaacactc tggcatgagt gggtccagtt caggcaggca ttgcgaaaac
      121 cgattcgaac ggagctgggc gctaacgggg cgatacggga actggaaaaa ttccgtcagc
      181 agggttttac acctgagcag gtgattcgac acagcatcgc caatgaatac cagggtctgt
      241 tcgcgccgaa aggtgttcgg cctgagacgt tgctccgaca ggttaacacc gtctcgttgc
      301 cggacagtgc gatcccgcca ggcttcaggg ggtaacagac catgaaaaat attgcgacag
      361 gaggcgttct ggagcgtatc cgcagactga ccccaccaca tgtaaccgcc ccattcagaa
      421 cggttgcgga gtggcgcgag tggcaacttg ctgaaggcca gaaacgttgc gaggagatca
      481 accgcctgaa tcgtcagttg cgggtggaaa aaatcctgaa ccgctccggc atccagccgt
      541 tgcaccggaa gtgttcgttt gcgaattacc gggcgcagaa cgacggtcag cgacatgcac
      601 tgagtcaggc gaaatccata gctgacgaac tgatgacagg ctgtacgaat tttgtgttca
      661 gcggtaagcc cggcaccgga aaaaatcatc ttgcagccgc gattggcaac cggctgatgg
      721 cgaaggggcg tagcgtgatt atcgtcaccg tgtccgatgt catgagcgtg ttgcatgaga
      781 gctacgacaa cggcaaatcc ggtgaaaaat ttttacagga gctttgtggt gttgacctgc
      841 tggtcctgga tgaaattggc atgcagcggg atacgaaaaa cgagcaggtg gtactgaacc
      901 agattgttga tcgccggacg gcatcgttac gcggtgtggg gatgctgaca aatattaacc
      961 atgcagcgat gaatacactt ctcggcgagc gggtgatgga tcgcatggtc atgaacggcg
//
LOCUS       DAJTUI010000279         1004 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTUI010000279 DAJTUI010000000
VERSION     DAJTUI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.283"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..534
                     /locus_tag="OO464_004270"
                     /pseudo
     CDS             1..534
                     /locus_tag="OO464_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          310 a          220 c          210 g          264 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggtg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      421 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      481 gttgttaccg gaaagtatgc agaccatctg ccattatacc gccaaagtga tctactattc
      541 cacacagcta tttagctctc tttttgttga gctcccatga ttctttcatt tattgttact
      601 aattatttta tgttatcatg atgttagaac acgctctgct ctgtgtctct attttatcta
      661 tcatattctg aggattaact acataacaag gcgcaagata tatatacaaa aacaaataat
      721 accgttgcaa taattccttt tagtaaagga ggtgtgatta atttcagggg atttcagagt
      781 aaaaattgta caacatatta aaatatgttc atttcgttat aatacacaca aggaaatatt
      841 aagttagata atatattcta aataaccata gataaaaacg cacataatgg ggaatatttt
      901 atgaaaatac ctgaagcagt aaatcatatt aatgttcaga ataatattga tcttgttgat
      961 ggaaaaacga atccgaataa agcaacaaaa gcattacaaa aaat
//
LOCUS       DAJTUI010000280          990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTUI010000280 DAJTUI010000000
VERSION     DAJTUI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.284"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            82..>990
                     /locus_tag="OO464_004271"
     CDS             82..>990
                     /locus_tag="OO464_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR6229149.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          301 a          206 c          228 g          255 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt
//
LOCUS       DAJTUI010000281          967 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTUI010000281 DAJTUI010000000
VERSION     DAJTUI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.285"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(3..485)
                     /locus_tag="OO464_004272"
     CDS             complement(3..485)
                     /locus_tag="OO464_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6229150.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="OO464_004273"
     CDS             complement(476..778)
                     /locus_tag="OO464_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6229151.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          294 a          236 c          195 g          242 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgc
//
LOCUS       DAJTUI010000282          962 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTUI010000282 DAJTUI010000000
VERSION     DAJTUI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.286"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..962
                     /locus_tag="OO464_004274"
                     /pseudo
     CDS             1..962
                     /locus_tag="OO464_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT          223 a          242 c          313 g          184 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ctgagcttcc
      481 tgaaagacgc gatacgttcg cggtggatgt ggaatatgcg gcaacggctt ctgaaagcgt
      541 ggtcagaggg gctggcgatg ccggagtcgt tgtcacatat cacgacggaa tcacagagga
      601 gaagcctggt gctgaaagcc ggaggaaaat actggcatgt gtacatgtcg aaaaaaacgg
      661 ccggcgggag gaatacggcg cgctacctgg gtcgttatct gaagaagccg ccgatagcgg
      721 cctcccggct ggcacattac aacggagggg gagcctgagc ttccgttacc tggaccacaa
      781 aacgggagaa acggcgacgg aaacgctgac acagcgtgag ctggtcgcga ggctgaaaca
      841 gcacatcccg gagaagtttt ttaagatggt gaggtacttc gggttccttg ccaaccgggt
      901 gtgtggagag aagctgccgc aggtgtaccg tgctctgggg atggataaac cggaaccagt
      961 gg
//
LOCUS       DAJTUI010000283          947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTUI010000283 DAJTUI010000000
VERSION     DAJTUI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.287"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(201..866)
                     /locus_tag="OO464_004275"
     CDS             complement(201..866)
                     /locus_tag="OO464_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR6229152.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          245 a          232 c          215 g          255 t
ORIGIN      
        1 gtaagaggct aacctacatg tgcaaagcat gaaattggcc tcagattaat gttaagcgtc
       61 ttgccggacg cgtaatgtta actggggctt ttctctatct gccttttggt gttcatgcct
      121 gaagcagata gcctcaagca cccgcagtca ttctacttac ctcgccaata tgaaatcaat
      181 cagaaaggtg ccccataaaa tcactccttc tcttctttac cgtagtggag ttgaccaatt
      241 ttgataagag ggcgtccctg agatttgcgg tgtagattgg tatcgcgcag agaatacaca
      301 cagccacaat attcctgctg atagaatttt tcgcgcttgc tgatttcaat catacgggac
      361 gagccgccct gcttgcgcca gttataatcc cagtacacca tgcccggata atgcgcgacg
      421 gctcgccgcc cacagtcgtt aacctgctgc atatttttcc agcgtgaaat gcccagtgaa
      481 ctgctgatca cactgaaacc attttcagcg gcgtacagcg ctgtccgctc aaaacgcatg
      541 tcaaaacaca tggtgcaacg gatccctcgt tcgggctccc attccattcc tttggctcgt
      601 tcaaaccagt tgtctgtgtc gtaatcagca tcaataaacg gcacgccgtg ttgttcagca
      661 aagcgaatat tctcatcctt acgaattaaa tactctttct gaggatgaat gttcgggttg
      721 tagaaaaaga tggtgtagtc gatttccgag gcctgaagcg cctccatcac ctcaccggaa
      781 catggagcac agcaagagtg cagtagtagt ttgtttgccc cgtttgggag ctccaatttg
      841 ggccgtttga aatcagcaac tgtcataaat atgtttattg aggtcataaa aatatcaaag
      901 agtgtagcat tagaacgggg ctatcggaaa cagatgtgtg actgctc
//
LOCUS       DAJTUI010000284          921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTUI010000284 DAJTUI010000000
VERSION     DAJTUI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.288"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..802)
                     /locus_tag="OO464_004276"
     CDS             complement(<1..802)
                     /locus_tag="OO464_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6229153.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNR"
BASE COUNT          220 a          230 c          236 g          235 t
ORIGIN      
        1 cccggttaac aagagtccat ccctgttttt ccagataaaa ccggaaagtc tccagggtac
       61 agaccagtgc gccatcagga acggtttcgg tgaattcgac attgccgaat ttgtcgaagt
      121 gaacaaccag agtgcgacca tcacccggaa tcatcttgtc tgcaggtggg gtgttattct
      181 ggcgcagttc ggcctccatg cggtcgaact cagcaatgta ggcttccttg aatgcggcgg
      241 cttttttgcc agtgaagccc atcaccagga aaacgaagcc gtttttggtg atttggtaca
      301 ttgggcgttt ttcgcctttg gcgtcggtat aggtgacggg cttaaaatta agccggttaa
      361 actccggcga gcattcgaga gtttcgatct tctgaataac gtttttgtgc attttccgga
      421 agaactccgc aaccgcaaca gacgtagtga cagcgcgacc attttcgatg gttgcgtcag
      481 ggtgagaaag ggtaaggata gtagccatga tggcagcctc gatagtcagt ttaaatgact
      541 caccaccaga ggtagcaatc tcatgggtgg tgagacgcac agggttgcta caaccggtga
      601 ctaccgaaac cggccagcct tgcggctgcc ctgcacgccc caccataatc tgaatgtggc
      661 tgtgcttgac gcataaaaaa accgcctgag cgcggttatg cgcagtagtc atcgtcgggg
      721 tagcaatccc ggcacccgtt ttatgaggtg caggtgcact ataattccac ccgttctggt
      781 tttcaatagc tacattcaac attttctctt tcctttcatc accgaagtga actttgttga
      841 tgcggtgcct ggtgcctcca ggtgacgtta accagttaac aattacagtt ggctttccca
      901 cccaaaccaa taaggactaa c
//
LOCUS       DAJTUI010000285          887 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTUI010000285 DAJTUI010000000
VERSION     DAJTUI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.289"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            270..743
                     /locus_tag="OO464_004277"
     CDS             270..743
                     /locus_tag="OO464_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6229154.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          270 a          186 c          197 g          234 t
ORIGIN      
        1 aatcgctgga ttcggtcatt ctgtctccct tgtatatggg ttaaccagat aaccacgggg
       61 agcaggtacc ggcatccacg ttacagacgg tcccggcaaa agtgcctgac ctgaccccta
      121 ttagcggtta ccagcgcccc accagacccg gtgacatcac cccccggatc atggacgact
      181 gggggcagta atcatgccgg gtctggctgg ctaacacccc attataaggg gtacgaataa
      241 agtaacgggg gggagatgaa ccctatgcaa tgtcctggtt aatatctcag tgtgctcatc
      301 agtgcactga taataaaaag acggagacag atgccattta cgataaagtt cggagttctt
      361 atttgctttc atgtattctg aaaaaaaaca aaaatgttgg gcttattctt catgcaccat
      421 catttgtttc tgtgtctgaa aaaatcgcca gaattgtgat ggcaaattac tcccgtaact
      481 ggagtaattc cgagttggcc agtgcggttt taatgagtga atcttcttta aaaagaagaa
      541 tgtacaaaga ggtcggttct ataagtacat ttgtacataa aataaagctt acagaagcta
      601 tcaggaaatt acgaaggaca aatactccca tttctgtcat atcatctgag ttaggttaca
      661 gttcaccatc gtatttttct aaagtcttct ttaaatattt aaagacatac cctcaaaaca
      721 taaggaagaa aaatggtcgg taatgtcacc aattaggagg acttgcagag ccgctttttt
      781 acgatactga gatcaaacag acataatcta tgcggagatt tacagcaggg catggttcac
      841 aaatctgaca gcgatgaatt atcggcactg cgggcagaaa atgctcg
//
LOCUS       DAJTUI010000286          853 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTUI010000286 DAJTUI010000000
VERSION     DAJTUI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.290"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(23..448)
                     /locus_tag="OO464_004278"
     CDS             complement(23..448)
                     /locus_tag="OO464_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229155.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(445..828)
                     /locus_tag="OO464_004279"
     CDS             complement(445..828)
                     /locus_tag="OO464_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229156.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          197 a          250 c          207 g          199 t
ORIGIN      
        1 tgagcaggtc gggcatttcg gattaagtac tcgcacctcc gcagtcctca aacaagtctg
       61 gctggtagct gtaaacagac ttcgtacata ccgctctgca ataaatcccc gtaccacagg
      121 cctcacagaa cttcttccca tgaaaaaact gctcacacat cacacaatgc cactccagca
      181 cgaccggtaa tggcgataaa aacatcgcca tatcctcaat gtaagggtgg gacttctccg
      241 gattcagcac cacacagtcc gccttctgtt gctcactcag ggcatgtaaa tcatgctcaa
      301 accacgcccc ctgagcatct gtctgcagaa tcaaccggcc atggcaggaa aggcagaaac
      361 actgccggat atttctgctg agcgcagagt cggcactgat aatgtgctca cccggcgtga
      421 tccccagccc cgtttttata ccgttcattc tgctcccccc tcctcactga agcgcccggt
      481 ctggcagtgt gtgcagtact gctccccata ataatcgtgg tgacattgcc tgcagtgcca
      541 gctggcttta cgtactacgg gtaaggtatc cgggacgaat tgctgcagac gtttaatcaa
      601 ccgtatttct ctgcgttccg gcctgacata agggcactca tgaccgtgct ccgtcagccc
      661 ttcgtcagcg tgttcaaacc agggccgttc agtgtcgtat tgcgggtggt atctgagtgc
      721 actgccgcaa aggtggcagg tgtagctatc gtaaggtgca gtctgtgcgg tacgggcacc
      781 ggtcagacgt ccgttgccat caagtgcgag aaaagatttt gcgtacatag tatatgttcc
      841 tcagccgcca gac
//
LOCUS       DAJTUI010000287          851 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTUI010000287 DAJTUI010000000
VERSION     DAJTUI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.291"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..244)
                     /locus_tag="OO464_004280"
     CDS             complement(<1..244)
                     /locus_tag="OO464_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229157.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIF"
     gene            269..508
                     /gene="relB"
                     /locus_tag="OO464_004281"
     CDS             269..508
                     /gene="relB"
                     /locus_tag="OO464_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR6229158.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            508..795
                     /gene="relE"
                     /locus_tag="OO464_004282"
     CDS             508..795
                     /gene="relE"
                     /locus_tag="OO464_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR6229159.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          235 a          172 c          217 g          227 t
ORIGIN      
        1 aaaaaataga tgaataagtt gagttgtgca tatgtagcct gaccgtcaca aagtatatgg
       61 tgtctgtacc agtaagatga tggccggact ctttaaaaaa gagctgacct gcacaataca
      121 ggatggactt agcaatggct gctcctggca caaagcggac agtgatcacc gttcttacga
      181 ctactttctg acttccttcg tgacttgccc taagcatgtt gtagtgcgat acttgtaatg
      241 acatttgtaa ttacaagagg tgtaagacat gggtagcatt aacctgcgta ttgacgatga
      301 acttaaagcg cgttcttacg ccgcgcttga aaaaatgggt gtaactcctt ctgaagcgct
      361 tcgtctcatg ctcgagtata tcgctgacaa tgaacgcttg ccgttcaaac agacactcct
      421 gagtgatgaa gatgctgaac ttgtggagat agtgaaagaa cggcttcgta atcctaagcc
      481 agtacgtgtg acgctggatg aactctgatg gcgtattttc tggattttga cgagcgggca
      541 ctaaaggaat ggcgaaagct gggctcgacg gtacgtgaac agttgaaaaa gaagctggtt
      601 gaagtacttg agtcaccccg gattgaagca aacaagctcc gtggtatgcc tgattgttac
      661 aagattaagc tccggtcttc aggctatcgc cttgtatacc aggttataga cgagaaagtt
      721 gtcgttttcg tgatttctgt tgggaaaaga gaacgctcgg aagtatatag cgaggcggtc
      781 aaacgcattc tctgaaccaa agcatgacat ctctgtttcg caccgaaggt gacacttctg
      841 ctttgcgttg a
//
LOCUS       DAJTUI010000288          851 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTUI010000288 DAJTUI010000000
VERSION     DAJTUI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.292"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            59..556
                     /locus_tag="OO464_004283"
                     /pseudo
     CDS             59..556
                     /locus_tag="OO464_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            597..788
                     /locus_tag="OO464_004284"
                     /pseudo
     CDS             597..788
                     /locus_tag="OO464_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          219 a          210 c          250 g          172 t
ORIGIN      
        1 gcgccagccc cgtcccgccc gatagagcgc ggcatagcgg gccccggatt acttgcgtgc
       61 gacggcagaa gaagccctga agcgaatcag tgaactgtac gccatcgagg atgaaatacg
      121 gggattacca gagtcagagt gtcttgcagt caggcagcag cgaagcaaag cgttactgac
      181 gtcgctgcat gaatggatgg tggagaagaa tggcacgctg tcgaaaaaat ccagactggg
      241 cgaagcattc agctatgtac tgaatcagtg ggacgccctc tgttattaca gtgatgacgg
      301 tctggcggag gcggacaata acacagcgga aagagcgctt cgtgcagtct gtctcggaaa
      361 gaaaaattac gtgttcttcg gtagcgatca cggcggcgag cgtggtgcac tgctgtacgg
      421 gctgatcggc acctgccgtc tgaacggtat cgatccggaa gcgtatctgc gccatattct
      481 gagcgtactg ccggaatggc cttccaaccg agttgacgac ctcctgccat ggaaggtggt
      541 gctaccatca ggataatcgt caatacggtg ttcggtgaac gtttacttgg agcatcatgc
      601 ttcatgaaga aaaactggca cgtcatcaac gtaaacaggc gatgtatacc cgaatggcag
      661 ccttcccggc ggtgaaaatg ttcgaagagt atgacttcac gttcgccacc ggagcaccgc
      721 agaagcagct ccagtcgtta cgctcactca gcttcataga gcgcaatgaa aacatagtct
      781 tactggggac atctgacatc acaaatcccc gggtggggat ttgtgtataa gagacagccc
      841 atggaggaag g
//
LOCUS       DAJTUI010000289          834 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTUI010000289 DAJTUI010000000
VERSION     DAJTUI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.293"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..>834)
                     /gene="ospC4"
                     /locus_tag="OO464_004285"
     CDS             complement(<1..>834)
                     /gene="ospC4"
                     /locus_tag="OO464_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR6229160.1"
                     /translation="DKIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLN
                     KTHHTVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVS
                     RYVHGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRII
                     NFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYS
                     LGNKKADIALYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVLEYFI
                     N"
BASE COUNT          264 a          140 c          118 g          312 t
ORIGIN      
        1 ttattgataa aatattctag cactttatag ttggcaccat ctttactaag taagtattca
       61 acatcataca gattacaata tctattgttt ttcattttct ccatctctgc gacatcttgt
      121 tttgtaaaat taaatttaga tagtaaataa agtgctatat cagctttttt attgcctaaa
      181 gaataagcaa gggcctgatg agccattttt ttatcagtca ctttattgtt aatctcttca
      241 taattagatg ctttaatcgc atcctccaaa gatatgtctc tgagttttat ttttttgaag
      301 ttatctgtac ttagcattct tggtatatga tactcaagct gaaacacaaa gtttataatt
      361 ctatccagac taacaggatc aatatttttg ttataacaaa actctctgaa tccttgatct
      421 ttactctttc ttataaaatc aataagatgc aacccaattc caagcctcat atctttagtg
      481 ttaaatattg gaacatcagt tttcccctga ttaccatgca catacctgct aacttcatcc
      541 acaacctctt taaagttatc taagaaaaat gattggtgct catggtaaaa aacaggtgga
      601 atagcattat caaagtgatc ggttaatgtc atatatccat atggagagtc atgatcaatg
      661 atatacgcat tggcgccaaa atctacagta tgatgtgttt tatttagtgg gagtttttct
      721 tgatgattgg aaatttcgac cccaaaaaaa acaaaatcgt ggttagagat ttctttaata
      781 tcctcagagt atgtattctt atgtgtttgt atattttcag actgaatttt atca
//
LOCUS       DAJTUI010000290          789 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTUI010000290 DAJTUI010000000
VERSION     DAJTUI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.294"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
BASE COUNT          242 a          168 c          160 g          219 t
ORIGIN      
        1 ttcgcgttgt gtgcctgctt ttaactacgt caggcgaggt ggttcctgtt attccccaac
       61 aacaagaaat ttgtataatc tgaatacccc aacaataaga agagtgagca agtgatgtat
      121 tagcgatgtc agctctcaaa gagactgcat caatgttaaa attattcaat gaagcaaaaa
      181 ctcaggctga ggttgatcgc gctgtgtctg aaataaactc taagttgtca agcattcagc
      241 ttgaatattt gtcatctcat tgatgagctg acaatctcta aaagcaatga ggctgcgctc
      301 aaagcaaaac tcacggagat agaaaagttt aagttccaaa gtgaagactg cgaacctcgc
      361 taattgatta ctggaacgat tgtgtatgcc agacagtgca atactagctc tggcaagatt
      421 accatgtatt tttgtccaga atgttttgcg aagagagcaa tatctatatt gcaacctttc
      481 cctatcgagg aaggcgataa atatacgaaa actcaatgcc cctcctgtaa aaatgagtat
      541 ctcatggact acaatttcaa taatgatttt tgagtcacgg tattcagatt tttaaagaac
      601 atgccggata ctcacccgtg tccggcgcac gcactccacc tcacccgtgg agaactcctt
      661 aattaccaac cttggcttcg ttggttagcc attaacgctg gtatgtaatc attctggcaa
      721 tgcttaattt gaagtggcac actgaatttg gccacctgaa cagaggtgat atgctcacct
      781 cagaacaac
//
LOCUS       DAJTUI010000291          783 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTUI010000291 DAJTUI010000000
VERSION     DAJTUI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.295"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..82)
                     /locus_tag="OO464_004286"
     CDS             complement(<1..82)
                     /locus_tag="OO464_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCR6229161.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAV"
     gene            452..>783
                     /locus_tag="OO464_004287"
     CDS             452..>783
                     /locus_tag="OO464_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229162.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          216 a          184 c          197 g          186 t
ORIGIN      
        1 atactgcatt tacaaggggt tgtgactctt cgacttccag ccgggtcagt cggttatttt
       61 ccagttgaaa tgcgctcagc atgaccggga ctcccaatgc ttaaaatatc ggacagttcg
      121 gtgggcacac agaaacaaat tgggtttcag accattaaac agcctgactc agcgcgacgg
      181 gaaaaattga ggtcgctgac aaccgctaag gctatcagca aaaagggata gccttaggag
      241 ttgatcctgg atgacaggat agtgagccag tatctactgg gtgtgtccaa ggcgaatgtc
      301 ctcttagagt gatcgtgcgc gcatgttacg ccagcaaaat tttgccgtca acacgcaaca
      361 gaacaccaca gaacaatagt tgaacttcgc gctttttggt actcgtcaag gtcgctaaca
      421 ggataaaaaa atcccacatc taggacctat aatggcgcat cggctactca ttggtaaagg
      481 aatgatcacc ttgaatctca aacggatttt tctcaccctc accttacttc ccttgtttgc
      541 tgttgccgct gatgattgcg cactctcaga tccgacgctg accgtacagg cgtataccgt
      601 taatcctcaa acagagcggg tgaaaatgta ctggcaaaaa gccaatggcg aagcgtgggg
      661 aacgttacat gctctgctgg cggatattaa tagtcagggt caggtgcaga tggcgatgaa
      721 cggcggcatc tatgatgaaa gctatgcgcc gctcggtttg tacatcgaaa acggtcagca
      781 gaa
//
LOCUS       DAJTUI010000292          781 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTUI010000292 DAJTUI010000000
VERSION     DAJTUI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.296"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            11..781
                     /locus_tag="OO464_004288"
                     /pseudo
     CDS             11..781
                     /locus_tag="OO464_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          217 a          193 c          211 g          160 t
ORIGIN      
        1 aggtgctcca atgaaaaaaa gaaattttag cgcagagttt aaacgcgaat ccgctcaact
       61 ggttgttgac cagaaataca cggtggcaga tgccgccaaa gctatggatg ttggcctttc
      121 cacaatgaca agatgggtca aacaactgcg tgatgagcgt cagggcaaaa caccaaaagc
      181 ctcccccatt accccggaac aaattgaaat ccgtgagctc aggaaaaagc tacaacgcat
      241 tgaaatggag aatgaaatat taaaaaaggc taccgcgctc ttgatgtcag actccctgaa
      301 cagttctcga taatcgggaa actcagagcg cattatcctg tggtcacact ctgccatgtg
      361 ttcggggttc atcgcagcag ctacagatac tggaaaaacc gtcctgaaaa accagacggc
      421 agacgggctg tattacgcag tcaggtactt gagctacatg gcatcagcca cggttcggcc
      481 ggagcaagaa gcatcgccac aatggcaacc cggagaggct accagatggg acgctggctt
      541 gctggcaggc tcatgaaaga gctggggctg gtcagctgtc agcagccgac tcaccggtat
      601 aaacgtggtg gtcatgaaca tgttgctatc cctaactacc ttgaaaggca gttcgccgtg
      661 accgagccaa atcaggtgtg gtgcggtgat gtgacctata tctggacggg taagcgctgg
      721 gcgtacctcg ccgttgttct cgacctgttc gcaagaaaac cagtgggctg ggccatgtcg
      781 t
//
LOCUS       DAJTUI010000293          763 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTUI010000293 DAJTUI010000000
VERSION     DAJTUI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.297"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            85..243
                     /locus_tag="OO464_004289"
     CDS             85..243
                     /locus_tag="OO464_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR6229163.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            327..>763
                     /gene="kbp"
                     /locus_tag="OO464_004290"
     CDS             327..>763
                     /gene="kbp"
                     /locus_tag="OO464_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6229164.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLR"
BASE COUNT          202 a          169 c          196 g          196 t
ORIGIN      
        1 actggcaggc tccgctaaaa ttactacgct taagagataa aatctctttt taaacaatga
       61 gtaattttct tatagggagt acatatgggt ttctggagaa tcgtcatcac catcattctg
      121 ccgccgatcg gcgtgctgct cggtaaaggg ttcggttggg cgttcattat taatattctg
      181 ctgacactgc ttgggtatat ccccggtttg attcacgcgt tctgggtgca aacccgcgat
      241 taaacctgtg ctcacccccg cggcgttaac gcgccgcgtt cctctgctac actttctgag
      301 tgtttccgtt aacgaatgag gtagctatgg gtctgttcaa ttttgttaaa gatgccggag
      361 aaaaactctg ggatgcggtt acaggtcagc acgataaaga cgatcaggcg aagaaggtgc
      421 aggagcatct gagcaaaacc ggtatacctg atgccgataa agtgaacatt caaattgccg
      481 acggcaaagc gacggtcact ggtgacggcc tgagtcagga ggcgaaggag aaaatccttg
      541 ttgcggtggg gaatatttcc ggtattgcca gtgttgatga tcaggtgaaa acggcgacac
      601 cagccactgc cagccagttt tataccgtta agtctggcga cactctgagt gccatttcca
      661 aacaggtcta cggtaacgct aatctgtaca ataaaatctt cgaagcgaat aaaccgatgc
      721 taaaaagccc ggataaaatt tatccggggc aagtgttgcg tat
//
LOCUS       DAJTUI010000294          762 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTUI010000294 DAJTUI010000000
VERSION     DAJTUI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.298"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..762
                     /locus_tag="OO464_004291"
                     /pseudo
     CDS             1..762
                     /locus_tag="OO464_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          183 a          191 c          225 g          163 t
ORIGIN      
        1 aaacgcagac ggcgtaccac acaggaaaag atcgcaattg ttcagcagag ctttgaaccg
       61 gggatgacgg tctccctcgt tgcccggcaa catggtgtag cagccagcca gttatttctc
      121 tggcgtaagc aataccagga aggaagtctt actgctgtgg ccgccggaga acaggttgtt
      181 cctgcctctg aacttgctgc cgccatgaag cagattaaag aactccagcg cctgctcggc
      241 aagaaaacga tggaaaatga actcctcaaa gaagccgttg aatatggacg ggcaaaaaag
      301 tggatagcgc acgcgccctt attgcccggg gatggggagt aagcttagtc agccgttgtc
      361 tccgggtgtc gcgtgcgcag ttgcacgtca ttctcagacg aaccgatgac tggatggatg
      421 gccgccgcag tcgtcacact gatgatacgg atgtgcttct ccgtatacac catgttatcg
      481 gagagctgcc cacgtatggt tatcgtcggg tatgggcgct gcttcgcaga caggcagaac
      541 ttgatggtat gcctgcgatc aatgccaaac gtgtttaccg gctcatgcgc cagaatgcgc
      601 tgttgcttga gcgaaaacct gctgtaccgc catcgaaacg ggcacataca ggcagagtgg
      661 ccgtgaaaga aagcaatcag cgatggtgct ctgacgggtt cgagttctgc tgtgataacg
      721 gagagagact gcgtgtcacg ttcgcgctgg actgctgtga tc
//
LOCUS       DAJTUI010000295          756 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTUI010000295 DAJTUI010000000
VERSION     DAJTUI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.299"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..499
                     /locus_tag="OO464_004292"
     CDS             <1..499
                     /locus_tag="OO464_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6229165.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            579..>756
                     /locus_tag="OO464_004293"
     CDS             579..>756
                     /locus_tag="OO464_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6229166.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQS"
BASE COUNT          198 a          173 c          178 g          207 t
ORIGIN      
        1 tgatggcgag attttaaaat ccgcttgtga aatcaatgac tctgataaga aaattgaagt
       61 tgctcttggt cactataatg ccgaacagtt tcgttctgtt ggcgatcgta gcccaaaaat
      121 tccattcact attcctttag ttaactgccc agtgactggg tgggagcacg ataacggcaa
      181 cgttgaagcg tctttccgtt tatggctgga aacgcgggat aatggcaccg tccctaactt
      241 ccctaatctg gcaaaagtgg gctcttttgc tggcacagcg acaacaggtg tgggtatccg
      301 tattgacgat gcggaaagcg gaaaccttat gccactgaat gctatgggga atgataacac
      361 gatttatcag atcccagcgg actccaatgg tattgtcaat gttgacctca tcgcttacta
      421 cgtatcaacc gttgaagcat cagaaatcac cccaggggaa gcagacgctg tcgttaacgt
      481 aacgctggat taccgttaat acgttacggc gttatctgac ctttcagata acgccctttt
      541 ccttcctctt tctcgttgta tcaggttgaa aaataactat gtcaaaacga acattcgcgg
      601 tgatattaac cttgttgtgt agcttctgta tcggccaggc gcttgcaggg ggtatcgttt
      661 tacagcgaac gcgagtgatc tatgatgcca gccgcaaaga ggctgcgtta cctgtcgcaa
      721 acaaaggcgc agaaacgcct tatttactgc aatcat
//
LOCUS       DAJTUI010000296          752 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTUI010000296 DAJTUI010000000
VERSION     DAJTUI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.300"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..>752
                     /locus_tag="OO464_004294"
     CDS             <1..>752
                     /locus_tag="OO464_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HCR6229167.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKRALFLSAFTALRDPLSRAYYTRKMSQGKR"
BASE COUNT          151 a          257 c          199 g          145 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgggc gttgttcctg tcggccttca ccgcgctcag ggatccgctc tccagggctt
      721 actacacccg caaaatgagt cagggaaaac ga
//
LOCUS       DAJTUI010000297          738 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTUI010000297 DAJTUI010000000
VERSION     DAJTUI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.301"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..648
                     /gene="ospC2"
                     /locus_tag="OO464_004295"
     CDS             <1..648
                     /gene="ospC2"
                     /locus_tag="OO464_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6229168.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNAKSDMALYLLSKFNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYK
                     VLEYFISNGLVDVNKRFQKANSGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          270 a           93 c          145 g          230 t
ORIGIN      
        1 aaaactgatg tgccaatatt taatactaaa gatatgaggc ttggaattgg gttgcatctt
       61 attgatttta taagaaagag taaagatcaa agattccgag agttttgtta taataaaaac
      121 attgatcctg ttagtctgga tagaattata aactttgtgt ttcagcttga gtatcatata
      181 ccaagaatgc taagcacaga taacttcaaa aagataagac tcagagatat atctttggag
      241 gatgccatta aagcatccaa ttatgaagaa attaacaata aggtgactga taaaaaaatg
      301 gctcatcaag ctcttgctta ttctctaggc aatgcaaaat ctgatatggc actttattta
      361 ctatctaaat ttaattttac aaaacaagat atcgcagaga tggagaaaat gaacaacaat
      421 atgtattgtg agctgtatga tgttgagtat ttacttagtg aagatagtgc caactataaa
      481 gtgctagaat attttattag taatggattg gttgatgtaa acaaaagatt tcaaaaagca
      541 aatagtgggg acactatgct tgacaatgca atgaaaagca aagattcaaa aacaattgat
      601 tttttattaa aaaatggagc ggtatcaggc aaacgatttg ggaggtgacc taaaatatat
      661 ttaggttgtg ccctgaatct gacgttgtca tttttgttat attgatcgat ataggggatg
      721 gtgcagaccg ttcccctg
//
LOCUS       DAJTUI010000298          732 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTUI010000298 DAJTUI010000000
VERSION     DAJTUI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.302"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..430
                     /locus_tag="OO464_004296"
     CDS             <1..430
                     /locus_tag="OO464_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229169.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
     gene            550..723
                     /locus_tag="OO464_004297"
     CDS             550..723
                     /locus_tag="OO464_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR6229170.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
BASE COUNT          205 a          169 c          175 g          183 t
ORIGIN      
        1 aagtcctgac acaaacgccg ccactgtaaa tcagacactt aaatgcgact caaaagcccg
       61 ggctgaccag cctggtgcat gtccaacaac gtacgagtta tacgaaggtg acgctaccta
      121 cagagctgct atcgacaaag cattaaaccc tgttgggtta aacggaatgt ttggggaaga
      181 cggctatatg gatggtcctg atggtggtgc gtatcctgta aacattaacg gaacgacctg
      241 ggtagaaggt ggcggttgta aagcccacgc ctgcggctgg gactatatcg taacactcta
      301 taacccaaaa acgcataaag tcgttggtta ctattacaac atcgaccctg gttacctgat
      361 ttggtttggt gaaactggcg ttcacgaatt cgcgtacctg gtcagggact acgtaaacaa
      421 aacaaattaa aggcgtaagt tatgcggttc atcttcgcgt tattaattta ttgatatcaa
      481 aagtaaatac ggctctgcca tgatgacagg gacttatatt ttcagggaac caaatacagg
      541 tctggttgta tgtcacgttt attgcccgaa gtccctttct cacgcgagca ggctgttgtc
      601 atcacaacag cttaccgcaa tgtgtttatc gacgatgacc agggaactca tttccgtcgg
      661 gttatccgca acgcagagac tgacgccgga aggttcgcca gtgacggtct tctcagacaa
      721 taaacctcat ta
//
LOCUS       DAJTUI010000299          731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTUI010000299 DAJTUI010000000
VERSION     DAJTUI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.303"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..282
                     /locus_tag="OO464_004298"
     CDS             1..282
                     /locus_tag="OO464_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCR6229171.1"
                     /translation="MFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLFESLCI
                     DHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            288..>731
                     /locus_tag="OO464_004299"
     CDS             288..>731
                     /locus_tag="OO464_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6229172.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVN"
BASE COUNT          208 a          166 c          187 g          170 t
ORIGIN      
        1 atttttgatc gtattgaaga aatggcatgg gctcgtcact accagcagat cgttcgtgaa
       61 gaaaaagaaa cggaactggc ggacgacctg gaaaaaggtc tgcctcagca cctgtttgaa
      121 tcgctctgca tcgaccattt gcaacgccac ggtgccaata aacaggcaat cagccacgca
      181 tttgatgatg atgttgagtt tcaggagcgc atggcagaac acatccggta catggctgaa
      241 accattgccc accaccaggt tgatattgat ttggaggact aaaatctatg gcgctttttc
      301 aacgagcaat aaacacacag gcctatctaa aagcaggaat tatggggttt gctggcgacg
      361 gcaaaaccta cacagcaagc gaactggcta ttggtctggt aatgcttatg cggcaacggg
      421 gaattgaagc tggtaaccgc ccggtaatgt ttctggacac agaaacaggc tctgactggg
      481 ttaaaccccg ctttgatgcg gaaaatatcg aattatttac ggcaaaaacc cgctcgtttg
      541 ttgatctgct tgaagccatt aatgaagcgg aaagcagcgg ttccgtaatg atcatcgact
      601 ccatcagtca cttctggacc ggattatgcg acgagtatgc cagacgccga aaccgtaaac
      661 gaggactgga attttcagac tgggcgtggc tcaaacagga gtggcgacgc tttaccgatc
      721 gatttgttaa c
//
LOCUS       DAJTUI010000300          723 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTUI010000300 DAJTUI010000000
VERSION     DAJTUI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.304"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..>723
                     /locus_tag="OO464_004300"
     CDS             <1..>723
                     /locus_tag="OO464_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6229173.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPS"
BASE COUNT          201 a          145 c          177 g          200 t
ORIGIN      
        1 tgagcgcatt ctgaccgacc gtgaaaatgg tgaaaaaggc gataattctc aagatacctc
       61 ttatgcccgt gtaggcgtga aaggtgaaac tcagatcaac cctgaaatga ctggttacgg
      121 tcagtttgaa ctagacctgg aagcatcaaa cagacacaac cctgaccaaa ctcgtttggc
      181 atatgctggt ttgagttaca aagattttgg ttcattcgac tacagtcgta atgtcggtgt
      241 cgcgtatgat gcagaagcat ttactgatat gtttgttgag tggggcggtg actcatgggc
      301 tggtactgac ctgttcatga ccaaccgtac caacggtgtt gcaacctatc gtaacacaga
      361 tttctttggc atggttgaag gtctaaattt tgctctgcaa tatcagggta aaaatgaagg
      421 tacaggcaac tacaaagcaa atggcgatgg ccatggttta tctgcaactt acactattga
      481 cggtttctca tttgctggtg cttatgcaaa ctctgaccgt actgattggc agtctggtga
      541 tggtaaaggc gaaagagccg aagtttgggc gttgtcaaca aaatatgacg ctaacaatgt
      601 ttatgctgct gtgatgtacg gtgaatctca caacatgaat tccgacgacg gtgatgttgt
      661 gaacaaaaca caaaacttcg aagctgttct gcaatatcag ttcgactttg gtctgcgtcc
      721 atc
//
LOCUS       DAJTUI010000301          722 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTUI010000301 DAJTUI010000000
VERSION     DAJTUI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.305"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            64..>722
                     /locus_tag="OO464_004301"
     CDS             64..>722
                     /locus_tag="OO464_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR6229174.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SA"
BASE COUNT          170 a          194 c          198 g          160 t
ORIGIN      
        1 cgggagacgt ggctttttca tttattacac cagtaaatct taaccaccga taaggagcaa
       61 attatgcgac tagccagtcg ttttggttat gctgcaaacc agatacgccg tgaccgtccg
      121 ctgacacacg aagaactgat gcactatgtt cccagtattt tcggtgaaga ccggcatact
      181 tcccgcagta aacgctatgc gtacactccc accatcaccg tactggaaag cctgcagcgg
      241 gaaggctttc agccattctt cgcctgccag acacgtgtgc gtgacccggg tcgccgggaa
      301 tacacaaaac acatgctgcg tctgcggcgg gccggacaga taaccggtca gcatgtccct
      361 gaaattattc tgctcaactc tcatgacggt tcatccagct accagatgtt acccggatat
      421 tttcgtgcca tttgtaccaa tggactggtc tgcggtcagt ctctgggaga attgcgtgtt
      481 ccacaccggg gaaatgtggt ggagaaagtt attgaagggg cttacgaggt ggtgggcgtg
      541 tttgaccgga tagaggagaa gcgtgatgcc atgcagtcgc tggtcctgcc gccaccggca
      601 cgccaggcgc tggcacaggc ggcactgact taccgttatg gtgacgaaca tcagccagtc
      661 accaccgctg acattctgac accacgacgc agggaggatt acggtaaaga cctgtggagc
      721 gc
//
LOCUS       DAJTUI010000302          720 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTUI010000302 DAJTUI010000000
VERSION     DAJTUI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.306"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..720
                     /locus_tag="OO464_004302"
                     /pseudo
     CDS             1..720
                     /locus_tag="OO464_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          183 a          187 c          169 g          181 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
//
LOCUS       DAJTUI010000303          716 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTUI010000303 DAJTUI010000000
VERSION     DAJTUI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.307"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..>716)
                     /locus_tag="OO464_004303"
     CDS             complement(<1..>716)
                     /locus_tag="OO464_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6229175.1"
                     /translation="SDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAA
                     LESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGL
                     SHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSL
                     LSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQL
                     DIRVKTDKTHYQPGELVNVE"
BASE COUNT          164 a          184 c          187 g          181 t
ORIGIN      
        1 cattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata
       61 tcaagctgag gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg
      121 gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg tacccgggct
      181 tcatacaggg tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc
      241 gactgctgtt ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg
      301 aaagtaatca gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata
      361 tctaccgtac cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg
      421 agaattaagc cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg
      481 acggtaaagg atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga
      541 cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac
      601 acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg
      661 ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcact
//
LOCUS       DAJTUI010000304          710 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTUI010000304 DAJTUI010000000
VERSION     DAJTUI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.308"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            44..397
                     /locus_tag="OO464_004304"
     CDS             44..397
                     /locus_tag="OO464_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR6229176.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(434..>710)
                     /locus_tag="OO464_004305"
     CDS             complement(434..>710)
                     /locus_tag="OO464_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="regulates the expression of formate dehydrogenase H
                     and hydrogenase-3 structural genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR6229177.1"
                     /translation="ENVIERAVLLTRGNVLQLSLPDIALPEPETPPAATVVAQEGEDE
                     YQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI"
BASE COUNT          179 a          179 c          189 g          163 t
ORIGIN      
        1 attaagatgt aatataaatg catcttaatg aggtggaggc agcatgtccg gcaagcgtat
       61 ctctcgtgaa aaactgacga ttaaaaaaat gatcgatctt tatcaagcga aatgcccgca
      121 ggcgtcagcg gagccggagc attacgaggc gttgtttgtt tacgcgcaaa agcggctgga
      181 taaatgtgtg ttcggcgaag agaaaccagc ctgtaagcag tgtccggtgc actgttacca
      241 gcctgcaaag cgtgaagaga tgaaacagat tatgcgttgg gccgggccga gaatgctgtg
      301 gcgtcatccg atcttaaccg tgcgtcatct catcgatgac aagcgtccgg tgccagaact
      361 gccggaaaaa tatcgtccga aaaaacctcg tgaatagtat gccggacagg cagatctgtc
      421 cggcaatttg cagttaaatc aatgccgatt tatcaattcc cagccgcttc atccgtgaca
      481 gcagggtcgt gcgtttcagc cccagacgtt gcgcagcgcc tttaggcccg gcaaccacgc
      541 tgttggtttc tttcagcacg cgcacaatca actgatattc atcttcgccc tcctgggcga
      601 caaccgttgc ggcaggcggc gtttcaggct ccggtaaagc aatatctggc aatgacagct
      661 gcagcacgtt gccgcgtgtt agcaatactg cgcgctcaat gacgttttcc
//
LOCUS       DAJTUI010000305          706 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTUI010000305 DAJTUI010000000
VERSION     DAJTUI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.309"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            27..299
                     /locus_tag="OO464_004306"
     CDS             27..299
                     /locus_tag="OO464_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229178.1"
                     /translation="MVRKFDELEAKCAAQENKVIPVSTELPPANESVLLFDANGEGWL
                     IGWRSLWYTWGQKETGEWQWTFQVGDLENVNITHWAVMPKAPEAGA"
     gene            299..604
                     /locus_tag="OO464_004307"
     CDS             299..604
                     /locus_tag="OO464_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229179.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            604..>706
                     /locus_tag="OO464_004308"
     CDS             604..>706
                     /locus_tag="OO464_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6229180.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQAT"
BASE COUNT          225 a          148 c          176 g          157 t
ORIGIN      
        1 aagtaaatga aacagatgct gaatacctgg tgcgtaaatt cgatgagctt gaagctaaat
       61 gtgcagcaca ggaaaacaaa gtaataccag tgtcaactga actgccacca gcaaatgaaa
      121 gtgttttgtt attcgatgct aacggagaag gctggctaat tggctggcgt tctctctggt
      181 acacctgggg acaaaaagaa accggagaat ggcagtggac atttcaggtc ggggaccttg
      241 aaaacgtcaa tatcactcac tgggcagtaa tgccaaaagc accggaggct ggagcataat
      301 gaccactttt accgacaaag aactgattaa agaaattaaa gagcgtatca gcagccttga
      361 cgtgcgagac gatattgagc gccgtgctta tgaaatcgca ctcctatctc tggaagtaga
      421 accagatgaa cgcgaagctt atgaattatt catggaaaag cgttttggtg acttagtaga
      481 tcgtcggaga gcaaaaaacg gcgataacga atacatggca tgggatatga ctctcggttg
      541 gatcgtctgg cagcaacgag ctggtatcca tttctcaaca atgtcacagc aagaggtgaa
      601 ataatggagc catacagcct cacactcgat gaggcctgtc attttctcaa gatatccaga
      661 ccgactgcca ttaactggat acgcacaggg cgtcttcagg caacac
//
LOCUS       DAJTUI010000306          702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTUI010000306 DAJTUI010000000
VERSION     DAJTUI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.310"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            135..674
                     /locus_tag="OO464_004309"
     CDS             135..674
                     /locus_tag="OO464_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR6229181.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
BASE COUNT          295 a          135 c          113 g          159 t
ORIGIN      
        1 atcaccttct aacatcatcc aaaaatataa aaacacaccc atcaccaaac atggacgtaa
       61 caagtcatcg tcgtttattc cagaaagacg gaataggatt gagccaaaca ggaaaagcaa
      121 cacaacttgt cattatgtcg catggtggat ggaaagagat ctcccatcca caaacactat
      181 tcacgcgtca aaaaggcgat ggatggactg tagtccccaa aaacctaaga attgattttt
      241 atacaaaaga caacgacttc acaaagggac taagcgttct ttcagaggta aacaaacgcc
      301 atactgaagc tcaaaaaggg ttaacaccat cactaaacat aagcaaagat gacctaaaag
      361 ttctagcgaa cgcgcgcaat atacctcaat caaaattaga agaagaaatg atgtcacaag
      421 ccatatatag aaaagaatac gcaacaagta atgagaagat aaaaaactat gctctatatt
      481 accatgaaca agcccaaaat attatacaaa gacatcaaga tggcttagga aacaaaaata
      541 ttgatatagc atttataact gataaaaatc ataagaagca cctttctgac atatttaaag
      601 taataaatga atcaggagta aaatatgatg taattcactt tggcgcttgc cgagtaaata
      661 gagaggagaa ataatcatca tttaccataa tcccttaatt gt
//
LOCUS       DAJTUI010000307          691 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTUI010000307 DAJTUI010000000
VERSION     DAJTUI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.311"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(135..265)
                     /locus_tag="OO464_004310"
                     /pseudo
     CDS             complement(135..265)
                     /locus_tag="OO464_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(263..358)
                     /locus_tag="OO464_004311"
                     /pseudo
     CDS             complement(263..358)
                     /locus_tag="OO464_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          200 a          125 c          166 g          200 t
ORIGIN      
        1 caagggaata tagtttgtgt aagcatatta cttccttatg ttatattttg gtaaaaaatt
       61 tattttttta cattatagtc ggctaaatga agtttactgt aaaaaaacga tactttttcg
      121 ggtggcgctt ccaatcagta gaatatgtgt ttttcaatga aggagccgat gacttttcgt
      181 ggatttccac ggaacgcgag aagcagattg ttttacgtgc cagccgcttg atgcgggtgc
      241 ggagggtcag gttgttacgt tcaagatata gcatgtgctg gatgaagatc ccttttctgg
      301 tcacctgttt atctttcgtg gccgacgggg tgacactgtt aaaatgtaat gaacgcatcc
      361 cacacaatta aacacggagt agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
      421 ctcgcagctt tccggcatca tactgcccaa tacgggatgc ataaagcgtc ctcagactgg
      481 agactccaca aatgaaatat acaccggttg gcgttgatat ggcaaaacat gtcattcaga
      541 ttcacttcat caatgagcac atgtcagcgc cagtgatata agacggtaat ggggatgcgg
      601 acgatttcct ggactctcag aagatgaaac atgtttactg aagaacaaaa aattcgcgct
      661 attgagcttt atttcaaata tggtaagaag c
//
LOCUS       DAJTUI010000308          678 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTUI010000308 DAJTUI010000000
VERSION     DAJTUI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.312"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            216..>678
                     /locus_tag="OO464_004312"
     CDS             216..>678
                     /locus_tag="OO464_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR6229182.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALA"
BASE COUNT          183 a          139 c          161 g          195 t
ORIGIN      
        1 ctgttttcct tgccaacaat cttgcaaaaa aaatgcgatt gctaaaacaa aagtgcaatt
       61 tgcggcaata tagttattcc atgaccgtac aatcttgcat tcagtgtaaa taatcttgtt
      121 tagctctcgc taaacgcggt tgtataaccg caatacacgc cgtattttat ccggcatata
      181 ttgtttttgc ttgctcatgg aatagggagg ttgtcatgta tcaacgctgt tttgataatg
      241 ccagtgaaac gctgtttgtc gccggtaaaa cgccacggct ttcacgtttt gcatttagcg
      301 atgatccaaa atgggagtct ggacatcacg ttcatgacaa tgaaaccgag ctgatttacg
      361 tcaagaaagg ggttgcaagg tttaccatcg attcttcgtt atatgtcgca catgcggatg
      421 acattgtggt gatagaacgc ggcaggctgc atgcggtggc ctctgacgtt aacgatccgg
      481 caacgacgtg tacctgtgcg ctgtacggct ttcagtttca gggggctgag gaaaatcagt
      541 tactgcaacc gcattcttgt ccggtaattg ccgcggggca gggaaaagaa gtcataaaaa
      601 ccttatttaa tgagctaagt gtgattttgc cgcaaagtaa aaatagccaa acatcttcgt
      661 tatgggatgc attagcct
//
LOCUS       DAJTUI010000309          671 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTUI010000309 DAJTUI010000000
VERSION     DAJTUI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.313"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..>671
                     /locus_tag="OO464_004313"
     CDS             <1..>671
                     /locus_tag="OO464_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR6229183.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGCLAHARR
                     KTHDEDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQSLYDWI
                     QLQRKT"
BASE COUNT          180 a          159 c          206 g          126 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcctggccca cgcccgccga
      481 aaaacccatg acgaggatgt gcgccgtccg acagaaatga ctcaggaagc gctcagacgg
      541 atagcagagt tatacgacat agaagcggag atacgtggca gtccggcaga ggaacggctt
      601 gcagtcagaa aagccagaag cgtccagttg atgcagtcgt tgtacgactg gatacagttg
      661 cagaggaaaa c
//
LOCUS       DAJTUI010000310          667 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTUI010000310 DAJTUI010000000
VERSION     DAJTUI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.314"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            148..654
                     /gene="mug"
                     /locus_tag="OO464_004314"
                     /pseudo
     CDS             148..654
                     /gene="mug"
                     /locus_tag="OO464_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT          163 a          150 c          189 g          165 t
ORIGIN      
        1 tctaaccact gagctaaggg gccatggtag cggattataa agtaactccg tgccgcaatc
       61 cagccattac cgcgcgcctg ctgtttttat aaacaatgta ttttcaatcc gttatacttt
      121 tcctggtgat gttagaaagg agtaaatatg gttgaggata ttttggctcc agggttacgg
      181 gtcgtgtttt gcggtatcaa ccctgggctt tcatccgccg ggactggttt tccctttgct
      241 catccggcaa atcgcttctg gaaggtgata tatcaggccg ggtttaccga ccgtcagttg
      301 aagccgcagg aggcacagca tctgctggat tatcgttgtg gcatcaccaa actggtagac
      361 cgtccaacgg tgcaagccaa tgaagtttca aagcaggagc tgcacgcagg tgggcgtaag
      421 ctgattgaaa aaattgaaga ttatcagccg caggcgttgg ctattctggg caaacaagca
      481 tatgaacagg gattcagcta gcgcggtgca cagtggggga aacaaacgct caccattggt
      541 tcgacgcaga tttgggtgct gccaaatccc agcggtttaa gtcgcgtttc actggagaaa
      601 ctggttgaag cgtatcgcga gctggaccag gcgctggtag tgcgtgggcg ataaaaaacg
      661 tcaccgg
//
LOCUS       DAJTUI010000311          659 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTUI010000311 DAJTUI010000000
VERSION     DAJTUI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.315"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            103..>659
                     /locus_tag="OO464_004315"
     CDS             103..>659
                     /locus_tag="OO464_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6229184.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          236 a          121 c          110 g          192 t
ORIGIN      
        1 agcaatacct ggagaaagag tacaaagcaa actgtattaa acttaataag ttatattgta
       61 tttcactaga gttcttatcg ttgataagaa attctggtta taatgattaa atcaacgaat
      121 atacaggtaa tcggttctgg tattatgcat caaataaaca acatacactc gttaactcta
      181 ttttctttgc ctgtgagttt gagtccatct tgtaatgaat attatttaaa agtctggagt
      241 gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa taggctaaaa
      301 atatgtttgc aaaatcaagg ggcggaatta aatctatctg agttagattt aaaaacatta
      361 cctgatttac cgcctcagat aacaacactg gaaataagaa aaaacctatt aacatatctc
      421 cctgatttcc caccaatgct taaggtaata catgctcaat ttaatcaact ggaaagctta
      481 cctgccttac ccgagacgtt agaagagctt aatgtgggtg ataacaagat aaaagaatta
      541 ccatatcttc ctgaaactct aactcattta cggatccata ataaccgatt gcatattctg
      601 ccactattgc caccggagct aaaattactg atagtttctg gaaacagatt agacagcat
//
LOCUS       DAJTUI010000312          648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTUI010000312 DAJTUI010000000
VERSION     DAJTUI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.316"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(141..647)
                     /locus_tag="OO464_004316"
     CDS             complement(141..647)
                     /locus_tag="OO464_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6229185.1"
                     /translation="MPVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGND
                     LKELRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRD
                     PSTPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDK
                     LVERLMQL"
BASE COUNT          171 a          158 c          142 g          177 t
ORIGIN      
        1 ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca agtgtccatg
       61 tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag cagtttatgt
      121 gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt atctctgcga
      181 acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga aataacaata
      241 tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata acagagaact
      301 cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg actgaaatag
      361 cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat tttgccgtgg
      421 cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga aactggattt
      481 tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac tttgaaaaag
      541 tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat acgcaccggc
      601 tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaatc
//
LOCUS       DAJTUI010000313          645 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTUI010000313 DAJTUI010000000
VERSION     DAJTUI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.317"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..409
                     /gene="virK"
                     /locus_tag="OO464_004317"
     CDS             <1..409
                     /gene="virK"
                     /locus_tag="OO464_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6229186.1"
                     /translation="NTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNISECLAVSE
                     HSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSDIASKKRS
                     GYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            474..>645
                     /locus_tag="OO464_004318"
     CDS             474..>645
                     /locus_tag="OO464_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6229187.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLS"
BASE COUNT          185 a          121 c          143 g          196 t
ORIGIN      
        1 aaataccgga cctgatgtta ttcggtgcgc aacccgggcc tgttatggga ttttcccaaa
       61 acggattatt tttgaagcat tttgtgcgtt gatgaaagct tgcaatatct ctgagtgtct
      121 ggcagtcagt gagcacagtc atgttttccg gcaattgaga tactggtatc agaaacgcaa
      181 aacctttgtt gcggtctata gtgatttctg ggagtctgta gcaggaaaaa cctgtggtga
      241 ctggtacaga ttaccaaccc aggtgattcg aaagccgcta agcgatatag ccagtaaaaa
      301 acgctctggg taccgaaaac gatacgcttt gctggattat atccatgaaa ctactatccg
      361 ctctttggat gcatatcctg tacactcaga acatcagaat ttaaattaag gtttgatgta
      421 ttctctgaat aaaacagtaa tgataatttt ggtagaggca ttcgcactaa ataatgaaaa
      481 aatataaatc tgagtttatt cctgaattta agaaaaatta cctttcccct gtttactggt
      541 ctacatggtt ccttttggga atgattgcag gtatttcaat gtttccccct tcattcagag
      601 atcctgtctt ggcaaaaata gggcgttggg cgggcagatt gagca
//
LOCUS       DAJTUI010000314          642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTUI010000314 DAJTUI010000000
VERSION     DAJTUI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.318"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            490..>642
                     /locus_tag="OO464_004319"
     CDS             490..>642
                     /locus_tag="OO464_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229188.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNVLRVTNSSS
                     SGISEKH"
BASE COUNT          235 a          115 c          128 g          164 t
ORIGIN      
        1 ttaatcccgt actctctggc cagttgggcc acgcaggccc ctagctggta agatttttca
       61 ataagatcaa gcttaaactc catagagtca tagagtaaga ggcacgaatc ctgcgggggc
      121 gaagagaatc tgacattgat agagtccatt aacgttataa gaggactcta tcatcgccaa
      181 tcatcagaca ttgaaataga cgacactgag atgacgctta caattctttt ggtttctgta
      241 ataagtaatc tgacatcccc cgtgcccaga agaggagagg gatatcaagc ccaagggtta
      301 ataattgata ctacggttaa aattgccttc agtaaaggaa acgtgattaa attcagggga
      361 tttcagagtg aaaaccatac aacatattaa aatctgttca tttagctata atacatacaa
      421 gaaagtgtcc agttagataa tgttatctaa ataaccacag ataaaaacgc acataattgg
      481 ggggggggga tgaaaatacc tgaagcagta aatcatatta atgttcagaa taatattgat
      541 cttgttgatg gaaaaacgaa tccgaataaa gcaacaaaag cattacaaaa aaatgtatta
      601 cgcgtaacaa actcatcttc ttctggcata agtgaaaaac at
//
LOCUS       DAJTUI010000315          635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTUI010000315 DAJTUI010000000
VERSION     DAJTUI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.319"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
BASE COUNT          181 a          117 c          140 g          197 t
ORIGIN      
        1 ttattgttgc aacttgcgta attattcagg tcagtttgga acgcactgta taaacataaa
       61 taacctgata ctgttgtgtg ttttcaaaga aaacacaggt atttttcaca taaaccttcc
      121 ccttttcata ttcgaccact taatcaaaaa acggttttat acttgttgcc cccactgggg
      181 ttaaatgtga gacatgtcgc actttgtgcc atgtgtcctc gctgatttta ggcgcggttc
      241 attcattctt tctgttggca taaagtatgg tatcggttgg gcgaatgaaa gggactcggg
      301 tcatggtcag acaatacctg aggcgttaat tcgttgaaag agattgtgaa tgggggaata
      361 actgagtctt aactgctgtg cgatagcccg gacagatagt tgctcgtcga atctgagacg
      421 taatgattct ggcaaataag tgcgaaagcc ggtgatatct agtgttgttt gagagttcat
      481 cgttcgcgtc cattaataca gatggacgcg attgttgcca gacaggataa ttttcacaag
      541 acatcgcaga tagagtactt acgataattt tttattttat acaacagatg cccacgaaaa
      601 gaattatttt caatagacac ctgcattaca caaag
//
LOCUS       DAJTUI010000316          632 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTUI010000316 DAJTUI010000000
VERSION     DAJTUI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.320"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            90..155
                     /locus_tag="OO464_004320"
                     /pseudo
     CDS             90..155
                     /locus_tag="OO464_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            383..631
                     /locus_tag="OO464_004321"
     CDS             383..631
                     /locus_tag="OO464_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6229189.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          198 a          130 c          147 g          157 t
ORIGIN      
        1 ctatagatgc acaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt ctaaagttaa
       61 actgggacct cgcgagcaag ggtgagacga tggcgcttta cacaattggt gaagtggcgt
      121 tgctttgtga tattaatcct gtcacgttac gcgcgtgacg caacattttt aaaaatcgac
      181 gaagatcgtc caggagcgcc gtttttcaag ggttggatag atgattcgtt taccatgtcg
      241 acgaacttcg acgaatttcg ccgataaaaa gtgtcccata catgccccat tgatgcccca
      301 taaacaatat caggaattga cactggttat ccatacagat aaaaataaca cctgtacata
      361 actacagtta caggagggtg ttatgcgtgt tgagatctgt atcgctaaag aaaaaatcac
      421 taaaatgcca aacggtgctg tggatgcgtt aaaggaagaa ttaacccgac gcatcagtaa
      481 acgttatgac gatgtagagg tgatcgtaaa agccaccagc aacgatggcc tttctgttac
      541 acgcaccgca gataaggatt ctgcaaaaac atttgttcag gagactctga aagatacctg
      601 ggagtctgct gacgagtggt ttgttcacta at
//
LOCUS       DAJTUI010000317          626 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTUI010000317 DAJTUI010000000
VERSION     DAJTUI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.321"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..>626)
                     /locus_tag="OO464_004322"
     CDS             complement(<1..>626)
                     /locus_tag="OO464_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HCR6229190.1"
                     /translation="GWLLLLAPFFFFTYGSLNQFTAVQDLNSHDIPSQVFGWETAIPF
                     LPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCGFFLYPLKFSFIRP
                     EVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVRWRKVCGGWFLLIA
                     ISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIKIAL"
BASE COUNT          210 a          152 c          147 g          117 t
ORIGIN      
        1 cagtgctatt ttgattcgac gttgatcaag tttctgataa ttccaacgac ggtcgacggg
       61 caccatccag tcaatcaaca tacctaccgc cagccctgtg atgacatcaa taaaatgatg
      121 ctgccatgtc gtcagcgtcg aaatggcgat gagtaaaaac catccgccac agactttacg
      181 ccacctcaca gccagatgct gacgaaagtg acgccaaagt agccagcaga gaataatatg
      241 cagcgaagga gactggttat aaggcagatc aaacagttca agttgcgaaa atagccatcc
      301 tgtcacccca ctcacttcag gacggataaa actaaacttc agcgggtaga gaaaaaaacc
      361 gcagcaggcc attaccgttg ccagaataag ccggtggaca agtcggcgtt gttcgaatgt
      421 cgagctacaa acgaacagcg aaaatccata taaaagatcc agactccagt aaggaacaat
      481 agtccaggga agaaaaggga tcgccgtttc ccaaccgaat acctgactgg ggatatcatg
      541 gctgttaagg tcctgaaccg cggtgaactg attaagagat ccataggtga agaagaaaaa
      601 cggggccagc aacaataacc agccag
//
LOCUS       DAJTUI010000318          624 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTUI010000318 DAJTUI010000000
VERSION     DAJTUI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.322"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            459..>624
                     /locus_tag="OO464_004323"
     CDS             459..>624
                     /locus_tag="OO464_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6229191.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGN"
BASE COUNT          215 a          114 c           98 g          197 t
ORIGIN      
        1 tgttgaaaca aatgacggta gagaatgtta acactgccgt taaacaatat ctttctcatc
       61 cggtaaatac ttataccgga gtattattgc caaaataata acagccccgt caacacatcg
      121 ttggcgggga ttttagcaat attcgctatt tttatgtaat aattttataa atgcgttcaa
      181 aataataacc aagtactaat agtgatattt taatacctga tttttacgtg ataattcagg
      241 aggcacagaa agcgcataaa aataacaaca taaaacacct tgccaccacc caagaattct
      301 ctattacatt gtttttaatg aaaaaatatc attccaacaa tatctcacga taaataacat
      361 taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg ataaatccta
      421 cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag caagtaacgg
      481 atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc actatcgcag
      541 aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt cgcatttcag attatcaatg
      601 atgaattata tcttgatggc aacg
//
LOCUS       DAJTUI010000319          611 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTUI010000319 DAJTUI010000000
VERSION     DAJTUI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.323"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..359
                     /locus_tag="OO464_004324"
     CDS             <1..359
                     /locus_tag="OO464_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229192.1"
                     /translation="VVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARYK
                     YRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYR
                     PLQKLINGTWYNVASI"
     gene            362..>611
                     /locus_tag="OO464_004325"
     CDS             362..>611
                     /locus_tag="OO464_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6229193.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDIT"
BASE COUNT          181 a          124 c          160 g          146 t
ORIGIN      
        1 ctgttgttgc agagcttggt gatgattcag gctggcattt tttcagccag agaaacccgg
       61 ataacagcat agtgttcact gttaacggac aggtaattcc gttaaattac ggaaacttcg
      121 atgcccgcta taaatatcga acagaggggg tacaggatgt acggtatggc catgaaatgt
      181 attacagccc cggcagtaac accgtttcgt ggagattttg cgcaccttcg ggacacgggc
      241 tgtcagggat ggcgatatcg gataccggcc gtaactcagc ggataacgtt gacggtgtgt
      301 attaccgacc actgcaaaaa ctgattaatg gcacctggta taacgtagcg agtatttaac
      361 aatgttgcat ttaaaaaata ttactgcggg taatccgaaa acagcagaac aatatcagat
      421 gacaaaacga tattcggtca cctggctttt ttcagaagac ggaaaaaact ggtatgaaga
      481 gctgaagaat ttcgccagcg acacaataaa aatagcttac accggagatg gtcgcgtggt
      541 gtgggtcggt aaggatgtga caggcatcga gccacgcaat gccagcgtta tcgaagttcc
      601 ggatattacc g
//
LOCUS       DAJTUI010000320          602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTUI010000320 DAJTUI010000000
VERSION     DAJTUI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.324"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            18..>602
                     /locus_tag="OO464_004326"
     CDS             18..>602
                     /locus_tag="OO464_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR6229194.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITR"
BASE COUNT          130 a          162 c          174 g          136 t
ORIGIN      
        1 gtcgccccgc ccttctgatg ctcccccgtt ttgccgatat ttttcagcag ggaaaccgct
       61 ggcttaactg gctggagaaa caaccggaag gggcagtccg tccggtggta attgaatcag
      121 tgacaaaaat catggcctgc gggaccacac tgatgggcta cacgcagtgg tgttgttcat
      181 caccggactg ttgccataca aaaaaggtct gcttccggtg taaaagccgc tcctgcccgc
      241 actgcggggt gaaggctggc gcacagtgga tacagtatct gctgagcctg gtcccctact
      301 gcccgtggca gcatattgtg ttcacacttc cctgccagta ctggtccctg gtgttccaca
      361 accggtggtt actggcagag atgagccgca ttgcagcgga tgtgatactg gaaatctgcc
      421 atcaggcaga tgtggagccg gggatattca cggtgatcca cacatggggg cgtgaccagc
      481 agtggcatcc gcatatccat ttatcgacaa ctgccggtgg tgtgacgtcg ggccacacct
      541 ggaaaaatct tcatttttac gcccgtaagg tgatgagcat gtggcgctac cggataacac
      601 gg
//
LOCUS       DAJTUI010000321          577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTUI010000321 DAJTUI010000000
VERSION     DAJTUI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.325"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..98
                     /locus_tag="OO464_004327"
     CDS             <1..98
                     /locus_tag="OO464_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6229195.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            156..512
                     /locus_tag="OO464_004328"
     CDS             156..512
                     /locus_tag="OO464_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229196.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          183 a          110 c          149 g          135 t
ORIGIN      
        1 ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg
       61 ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc
      121 aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt
      181 taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag gttaattttc
      241 tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag
      301 agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct
      361 ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac
      421 aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga
      481 tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat
      541 ccttaaccgg agggatttct gcaccctcaa atcatca
//
LOCUS       DAJTUI010000322          571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTUI010000322 DAJTUI010000000
VERSION     DAJTUI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.326"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(131..>571)
                     /locus_tag="OO464_004329"
     CDS             complement(131..>571)
                     /locus_tag="OO464_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6229197.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          144 a          159 c           97 g          171 t
ORIGIN      
        1 tttaacgtcc tgtgccgttt ttcaggtata aaaaaaaccg cataaagcgg gctctttcaa
       61 atgtccatgt ccgctattcg cctcgcggta cagctttgcg aagcgtacaa gaattgaagc
      121 agtttttacg tcaaaaagca ataacttttt tctctatacc aaaagccata accattggtt
      181 tgtacaaaat aaattctgcc acctttagcc aatgctcaat gcgtctttca caggttctta
      241 aactccattt cgggtgtgca tcattcagca gttcagccat tttgcatttg gtcatccccc
      301 gcccctcata ccgttgccga aggacgctaa tcaatcctgg atgctctgcc agcacctcac
      361 ttatgacccg atcaatacat aacgcctctg catcagtaca atgcgccagc cagctctttt
      421 gcttaccgtt aatcatatcc cgcaaaaaag cctcaagttc agacttgttc agacctgctt
      481 ttttcatcct ccggagcgcc tcgttaattg ccgtttttgt cagcttttta gaggtcaaca
      541 tcaggttaaa catattcccc gtcttaccgc c
//
LOCUS       DAJTUI010000323          555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTUI010000323 DAJTUI010000000
VERSION     DAJTUI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.327"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(52..555)
                     /locus_tag="OO464_004330"
                     /pseudo
     CDS             complement(52..555)
                     /locus_tag="OO464_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
BASE COUNT          123 a          132 c          142 g          158 t
ORIGIN      
        1 gctgcttaaa aaaagaacaa atggtagtgt ccgctattgc cagtacacct cagccttaat
       61 tgcatcagtc aggcggagca gggcggcatt atccagagtt gtcggttctg ccatgccgcg
      121 tatggccaac agcagattct ggacatagct gttttcctga tccatctcaa gagcagtaat
      181 gtgtttgcgt tgttcacggg tggcatgatg caggtatttc agatccccgg cagcatacgt
      241 aaaaatgtgc agaagacgct gtgtgaaccg caaagtggct acagagactt cgcaatcctg
      301 gcaatcctcg tgggcgtctg cctgcgcgtt ttcttcctgg cctcccgcca gttctttggt
      361 ttcttgagca ttatcctggt ggtgaacgtc gtctggcgct gctcccggtt ttagttccca
      421 tgtcatggag tctttgctga gttgatagcg ttcactccag gtaaaatcga tctcaccttc
      481 agggggaagg tcattaacga caggaaaatt tgtggcaaca gctttaaaat agttgctcag
      541 ttttttacct gactt
//
LOCUS       DAJTUI010000324          551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTUI010000324 DAJTUI010000000
VERSION     DAJTUI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.328"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..551
                     /locus_tag="OO464_004331"
                     /pseudo
     CDS             1..551
                     /locus_tag="OO464_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          137 a          154 c          153 g          107 t
ORIGIN      
        1 cagcattcgc ctgaatttcg cagtgaagcc ctgaagcttg ctgaacgcat cggtgttact
       61 gccgcagccc gtgaactcag cctgtatgaa tcacagctct acaactggcg cagtaaacag
      121 caaaatcagc agacgtcttc tgaacgtgaa ctggagatgt ctaccgagat tgcacgtctc
      181 aaacgccagc tggcagaacg ggatgaagag ctggctatcc tccaaaaggc cgcgacatac
      241 ttcgcgaagc gcctgaaatg aagtatgtct ttattgaaaa acatcaggct gagttcagca
      301 tcaaagcaat gtgccgcgtg ctccgggtgg cccgcagcgg ctggtatacg tggtgtcagc
      361 ggcggacaag gataagcacg cgtcagcagt tccgccaaca ctgcgacagc gttgtcctcg
      421 cggcttttac ccggtcaaaa cagcgttacg gtgccccacg cctgacggat gaactgcgtg
      481 ctcagggtta cccctttaac gtaaaaaccg tggcggcaag cctgcgccgt cagggactga
      541 gggcaaaggc c
//
LOCUS       DAJTUI010000325          548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTUI010000325 DAJTUI010000000
VERSION     DAJTUI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.329"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..376)
                     /locus_tag="OO464_004332"
     CDS             complement(<1..376)
                     /locus_tag="OO464_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR6229198.1"
                     /translation="MLNVAIENQNGWNYSASAPHKTGAGRGNPNVTRAHSRAEAVFLC
                     VMHSSIQIMVGCAGQSQDWPGSRVTGISTPVRLTTLMVVENLGGELINLSLEDAIMAT
                     IPALSHPDVTIENGRAVTTSVAV"
BASE COUNT          136 a          147 c          133 g          132 t
ORIGIN      
        1 caaccgcaac agacgtagtg acagcgcgac cgttttcgat ggttacgtca gggtgagaaa
       61 gggcagggat agtagccatg atggcatcct cgagtgataa gttaattaac tcaccaccga
      121 ggttttccac gaccataagg gtggtgagac gtacaggggt ggaaataccg gtcactcgag
      181 aacccggcca gtcttgcgac tgccctgcac atcccaccat aatttgaatg gagctgtgca
      241 ttacgcataa aaaaaccgct tcagcgcggc tatgcgctcg agtaacgttc gggtttccac
      301 gcccggcccc cgttttatga ggtgcagatg cactataatt ccacccgttc tggttttcaa
      361 tagctacatt caacattttc tcttaccttt catcaccgaa gtgaactttg ttgatgcggt
      421 gcctggtgcc tccaggtgac gttaaccagt taacaattaa cgccggatac agggaaaccc
      481 ataaccaccc gatacgtggg aactaatcgc tttttaactg ttccgcgtgc gctcagccgc
      541 attcaccg
//
LOCUS       DAJTUI010000326          545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTUI010000326 DAJTUI010000000
VERSION     DAJTUI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.330"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..>545
                     /locus_tag="OO464_004333"
     CDS             <1..>545
                     /locus_tag="OO464_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6229199.1"
                     /translation="LATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGIT
                     AVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVMT
                     AIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKRT
                     VPLATMLGTGLAGIVYIAATQV"
BASE COUNT          114 a          121 c          135 g          175 t
ORIGIN      
        1 ctggcaacaa aaaacccgca acaaggtggc ccaattgctt atgccggaga aatttcccct
       61 gcatttggtt ttcagacagg tgttctttat taccatgcta actggattgg taacctggcg
      121 attggtatta ccgctgtatc ttatctttcc accttcttcc cagtattaaa tgatcctgtt
      181 ccggcgggta tcgcctgtat tgctatcgtc tgggtattta cctttgtaaa tatgctcggc
      241 ggtacctggg taagccgttt aaccactatt ggtctggtgc tggttcttat tcctgtggtg
      301 atgactgcta ttgttggctg gcattggttt gatgcggcaa cttatgcagc taactggaat
      361 actgcggata ccactgatgg tcatgcgatc attaaaagta ttctgctctg cctgtgggcc
      421 ttcgtgggtg ttgaatccgc agcagtaagt actggtatgg ttaaaaaccc gaaacgtacc
      481 gttccgctgg caaccatgct gggtactggt ttagcaggta ttgtttatat cgctgcgact
      541 caggt
//
LOCUS       DAJTUI010000327          544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTUI010000327 DAJTUI010000000
VERSION     DAJTUI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.331"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..>544)
                     /locus_tag="OO464_004334"
     CDS             complement(<1..>544)
                     /locus_tag="OO464_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6229200.1"
                     /translation="AAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFSKDA
                     NQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLMGRS
                     EEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAMKKG
                     ALKSADTLQKVTQELNKQITA"
BASE COUNT          147 a          133 c           98 g          166 t
ORIGIN      
        1 tagcggtgat ttgtttattc agttcctgag tgaccttctg aagagtatcg gcagatttta
       61 aagccccctt cttcatagca tcaaataatt cctgttcagt ctttccggtt gctttagcga
      121 atacctgaac cataccagga acagcttcag ctagttgccc cttaagctct tcagccataa
      181 tctgaccctt accagccatc tgtgataatg ctgtcagtgc tcgcttaatc tcttcttcac
      241 ttcgccccat cagacgagaa tagccaatca tatttctaaa aagttcttca gaaccttgaa
      301 tgcctagaga ctctctagct gaagcaccga aaccagcaaa ctgtgattgc agttcagcga
      361 tatctaaacc cgttgtttga gctaatgact ttgtgaaatc ttttacttga tttgcatctt
      421 tagaaaatac gaaatcaatt gaacgttcag cacttgcacg ttgaaggcca acttccatta
      481 ctcgctgata agcggatagt acagcctgaa agctgataag tacacctgct gttttaactg
      541 ctgc
//
LOCUS       DAJTUI010000328          544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTUI010000328 DAJTUI010000000
VERSION     DAJTUI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.332"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..230
                     /locus_tag="OO464_004335"
     CDS             <1..230
                     /locus_tag="OO464_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6229201.1"
                     /translation="GENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEP
                     EDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
     gene            239..544
                     /locus_tag="OO464_004336"
                     /pseudo
     CDS             239..544
                     /locus_tag="OO464_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          149 a          131 c          144 g          120 t
ORIGIN      
        1 tcggtgaaaa cgcgttacga tcggtggcgg ttggaagaaa aaattatctc tttttcggct
       61 cagacaaggg aggagaaagt gcggcgatca tctacagtct gctggtcacc tgcaaacaga
      121 acgaagtgga gccggaggac tggttgcgcg aagtgatcga gaagctcaat gactggccgt
      181 cgaaccaagt gcatgaactg ctgccctgga acttctcgtc tgtaaaataa tccttacgct
      241 acgtacttct cggggcgctt acgttgatgc agaagccata tgtgaagcgg cttcacgacc
      301 atcaatgcgt tttgtccagc caagaactga gacccagcaa gcaataaggg cattacaccg
      361 ggtcagagaa tcgctcatca gagataaggt gaaaacaacc aaccagatcc acggtttcct
      421 gctggaattt ggaatcagcc tgccgacagg ggatgccgta ataaaacggt tgtctctcgt
      481 attggctgaa cacgaaatcc ccgaatatct gagccgcctt ttggtgaggt tgcataccca
      541 ctac
//
LOCUS       DAJTUI010000329          542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTUI010000329 DAJTUI010000000
VERSION     DAJTUI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.333"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(186..>542)
                     /locus_tag="OO464_004337"
     CDS             complement(186..>542)
                     /locus_tag="OO464_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6229202.1"
                     /translation="SICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQ
                     AVKAELFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTR
                     SEQKAAFSKQSIAKVD"
BASE COUNT          141 a          138 c           97 g          166 t
ORIGIN      
        1 taaagggccg gaggcagaat aataaccata tgtcatcaca tctgcaaact catctgacca
       61 ccagcacgtt taacgtcctg tgccattttt caggcataaa aaaaccgctc ggcggcgggt
      121 tttgcttact ttaccatcgc gtacaaaatc ggcaaagtat cagatttgca tgagatatat
      181 gccttttaat ctacttttgc aatactttgc tttgaaaatg ccgctttttg ttctgaacgt
      241 gttctcatca caaacaataa agcctcacta tccagtcggt gaaaaatgtg tttcattaca
      301 acccagtgac cagtaaatgt tttggaccag tttttggttg ttactcccgc cagtaatgcc
      361 agctcctgat attcataacc ttccccacca aaaagttctg cttttaccgc ctgcgccgcc
      421 agccagatta attttttcag gcgttcctgt gttttctctg caattttcct agtgccagaa
      481 tgagccttaa actcttccca cgcccagtgg gttatggtga tctgatactc ccagcaaata
      541 ct
//
LOCUS       DAJTUI010000330          541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTUI010000330 DAJTUI010000000
VERSION     DAJTUI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.334"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..541
                     /locus_tag="OO464_004338"
                     /pseudo
     CDS             1..541
                     /locus_tag="OO464_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          125 a          142 c          161 g          113 t
ORIGIN      
        1 gccgtaaaac atggcaactg cagttcactt acaccgcttc tcaacccggt acgcaccaga
       61 aaatcattga tatggccatg aatggcgttg gatgccgggc aaccgcccgc attatgggcg
      121 ttggcctcaa cacgattttc cgccatttaa aaaactcagg ccgcagtcgg taacctcgcg
      181 catacagccg ggcagtgacg tcatcgtctg cgcggaaatg gacgaacagt ggggatacgt
      241 cggggctaaa tcgcgccagc gctggctgtt ttacgcgtat gacaggctcc ggaagacggt
      301 tgttgcgcac gtattcggtg aacgcactat ggcgacgctg gggcgtctta tgagcctgct
      361 gtcacccttt gacgtggtga tatggatgac ggatggctgg ccgctgtatg aatcccgcct
      421 gaagggaaag ctgcacgtaa tcagcaagcg atatacgcag cgaattgagc ggcataacct
      481 gaatctgagg cagcacctgg cacggctggg acggaagtcg ctgtcgttct caaaatcggt
      541 g
//
LOCUS       DAJTUI010000331          540 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTUI010000331 DAJTUI010000000
VERSION     DAJTUI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.335"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..401
                     /locus_tag="OO464_004339"
     CDS             <1..401
                     /locus_tag="OO464_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6229203.1"
                     /translation="KITYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGY
                     WFYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYA
                     KSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            425..>540
                     /locus_tag="OO464_004340"
     CDS             425..>540
                     /locus_tag="OO464_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6229204.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVA"
BASE COUNT          142 a          126 c          142 g          130 t
ORIGIN      
        1 ttaaaataac ttacaccgga gacggtcgcg tggtgtgggt cggtaaggat gtgacaggca
       61 ttgaaccccg taacgccagt gttattgaag ttcctgatat taccgccaac cgccgcatta
      121 ccgcgccggg ttactggttt taccgcaatg atgaatttgt ctttgactac agactcaaag
      181 cggaagatga gcgtgatgcc cttctggctc aggtcagtgc ccggacaggg gaatgggaag
      241 aagacctgct gctggggtta atcagcgacg aagataaaga aaagctgaaa gcctgtcgta
      301 tttacgcgaa atcgctgcag gcgatggatt tcagcaccat cactgataaa tcctcataca
      361 acgccattga atggcccgcc tctccggaag gttcttcctg atttaattta tcgcgagaaa
      421 aacaatgtct gtagtgatat caggtgcgct gactgatggc gcaggtatcc ccatgtccgg
      481 ataccatatt attctgaaat cccgggtaaa caccccggaa gtggtgatga acactgttgc
//
LOCUS       DAJTUI010000332          539 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTUI010000332 DAJTUI010000000
VERSION     DAJTUI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.336"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(46..480)
                     /locus_tag="OO464_004341"
     CDS             complement(46..480)
                     /locus_tag="OO464_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6229205.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
BASE COUNT          129 a          164 c          116 g          130 t
ORIGIN      
        1 accatccctc ttcagtcagc agccttctgg cgcgcagaac gtgtgtcata ttccttcctc
       61 gaccggacat tcttcatggc agcggcctaa ctcgtccacc agactggtca gccctcggtg
      121 tccacactcc agcgcctgta cacggaattc ttcaccgctt aacccttcgc gctccagcac
      181 catctccagt agcagttgca aattggtgcc ggtaatcact tcacagcccg gtttttgcat
      241 cgccagcgtt gaagctacgc gaaacggcgt gccgcccagc aaatccgtca gaaaaacaat
      301 gccgtcttca caatccagtt gggcgatggc ctcttcaagc tgcgatgtaa gcagcgcggc
      361 gcttgaggtt tcgggaaaat cgatggcaat aaactgcgat tgatcgccga ggatttgctt
      421 cattgctttt tccatgccac tggcaaaccc gccatgccct gtcaaaataa tacttaacat
      481 ctaatacctc attgtattta aaggaaatgg cagaatttac cgacgatccc tagaactac
//
LOCUS       DAJTUI010000333          538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTUI010000333 DAJTUI010000000
VERSION     DAJTUI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.337"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..443
                     /locus_tag="OO464_004342"
     CDS             <1..443
                     /locus_tag="OO464_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6229206.1"
                     /translation="RGAKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKP
                     MCENTVNKALRVMGYDTKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVR
                     MAYIHKAEHLEARKAMMQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT          161 a          130 c          130 g          117 t
ORIGIN      
        1 gccgcggggc aaaaatgcga atgccgcata tcgtccccct ctcagaacag tccatcgcca
       61 ttctgaaaca gattaaggat atcaccggta ataatgaact gatcttcccc ggcgaccata
      121 acccgtataa gccaatgtgt gaaaacacgg tcaataaggc actgcgggtg atgggttacg
      181 acacgaaaaa ggatatctgc ggtcacggct tccgggcaat ggcatgcagt gcgctgatgg
      241 aatcgggttt atgggcaaag gacgcagtag aacgccagat gagtcatcag gagcgcaata
      301 ccgtgcgcat ggcttatatt cataaggcag agcacctgga agcccgcaaa gcgatgatgc
      361 agtggtggtc ggattatctg gaagcatgcc gagaatctta tgcaccgcct tatacaattg
      421 gtaaaaataa gtttatccca tagtatacta acgaatccca cactctggca aattaccacc
      481 acctctacag gaggtggttc tatatagact tttatcaact tcatgacgaa aaacacct
//
LOCUS       DAJTUI010000334          524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTUI010000334 DAJTUI010000000
VERSION     DAJTUI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.338"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            18..>524
                     /locus_tag="OO464_004343"
     CDS             18..>524
                     /locus_tag="OO464_004343"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HCR6229207.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALA"
BASE COUNT          158 a          100 c          127 g          139 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggca
//
LOCUS       DAJTUI010000335          513 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTUI010000335 DAJTUI010000000
VERSION     DAJTUI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.339"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..>513
                     /locus_tag="OO464_004344"
     CDS             <1..>513
                     /locus_tag="OO464_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6229208.1"
                     /translation="RHIIIGNEGALCYITEVTVKIFKYTPENNLFYGYILEDMKTGFN
                     ILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEI
                     VARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEI
                     YESVINRIRT"
BASE COUNT          139 a          130 c          128 g          116 t
ORIGIN      
        1 ttcgtcacat catcatcggc aacgaaggtg cattgtgcta tatcactgaa gtaacagtga
       61 aaatctttaa atacaccccg gaaaacaacc tcttctacgg ctatatcctg gaagacatga
      121 aaaccggctt caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc
      181 gtttgtatga cgctgaagat ggcacccaac acttcaccca ttttgccgac ggaaaatgtg
      241 tgctgatctt tatggctgaa ggtaaccctc gcattgcgaa ggcgacgggc gaagggattg
      301 cggaaatcgt tgcccgctac ccgcagtgcc agcgcgtgga cagcaaactg attgaaacct
      361 ggtttaacaa cctgaactgg ggaccggata aagtggctgc cgaacgtgtg cagatcctca
      421 aaaccggcaa catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa
      481 tctacgaaag cgttattaac cgtattcgta ctg
//
LOCUS       DAJTUI010000336          506 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTUI010000336 DAJTUI010000000
VERSION     DAJTUI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.340"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(42..242)
                     /gene="glgS"
                     /locus_tag="OO464_004345"
     CDS             complement(42..242)
                     /gene="glgS"
                     /locus_tag="OO464_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR6229209.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          161 a          119 c           82 g          144 t
ORIGIN      
        1 atctaataca aacactaaaa cgggccatca ggcccgttat atcagtgctc taactccagc
       61 tctcttgcct gcatcatctg ttggcaatac caggcataac gtgcgcaaaa ccaagttcta
      121 tgttcttcat ccatgttacc agtaacagcc agaatatcga ccgggcgacc ttctgcgtgc
      181 attctggcaa aactacgcgc caggaaatcg aaattattca aagaattaag actatgctcc
      241 ataagcacat cctccggtta tcgcatacct tttaaggcca tatgcttttt ctgatttcag
      301 tattccccaa tcagtgtagt gcctttaaac ataacgtgcg taaatatatt gtccccgatc
      361 acacttttta gtgaaaaata aaaaaccata ttaaaatact ttctcttcca gaacttctct
      421 aacatgtgtt attattaaca aagacaaatg taagcaggga aactattcca aggtaaatat
      481 tgcgtcacta aatggacatt ggagtg
//
LOCUS       DAJTUI010000337          502 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTUI010000337 DAJTUI010000000
VERSION     DAJTUI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.341"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(11..>502)
                     /gene="queE"
                     /locus_tag="OO464_004346"
     CDS             complement(11..>502)
                     /gene="queE"
                     /locus_tag="OO464_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR6229210.1"
                     /translation="TKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLLPL
                     TDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKHPV
                     GRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHKYL
                     NIA"
BASE COUNT          114 a          140 c          112 g          136 t
ORIGIN      
        1 aaatgtgtaa tcaggcaata tttaaatatt tatgtgtttg catcgacaaa cgccaattac
       61 gcgcaataca ggtttcaatg cacaatcgtg tggcatcatc cttttggcta atcggctgca
      121 gtgcaatgac tcgcggttta tcatcggtca gcgttgccag tagttcatcc agtgcttcaa
      181 tatcgcgtac gcgccccact ggatgcttga tttcgttggc tcgctccagt gcctgtgaca
      241 acacttcata gccgccgcgc atgttcagct ttggcgatac ggtaacccag gtattcggtg
      301 tgcagcgtac ttcatgagtt ccactggttt cgatctggca gctaaaaccg ttcttttcga
      361 gcaggtcagt cagtggcagc aaatcatgaa tgcaaggctc accacccgta atcaccacat
      421 gccgcgcggt atatccctga cgaccaatga cagccagcaa gtcttcactg ctcgccgccc
      481 cccacttatc actctctttg gt
//
LOCUS       DAJTUI010000338          491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTUI010000338 DAJTUI010000000
VERSION     DAJTUI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.342"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            120..>491
                     /locus_tag="OO464_004347"
     CDS             120..>491
                     /locus_tag="OO464_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6229211.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGIQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAE"
BASE COUNT          170 a          105 c          119 g           97 t
ORIGIN      
        1 gatcccacta agaataaatc tccttacctc acaacacgac aagcctgcat tgcggctctt
       61 cagtctccgc tgcatactgt ccaggtgagc gcgggtgatg gcataacaga ggaaagaaaa
      121 tgtcactctt ccgcagaggt gaaatatggt acgccagttt cacattgccg aacggtaaaa
      181 gatttaaaca gtctcttgga acaaaggaca aaaggcaggc gacagaactc catgacaagc
      241 taaaggctga agcatggcgg gtcagcaaac ttggtgaaat acctgatata acgttcgagg
      301 aagcgtgtgt caggtggctt gaagagaaag cacataaaaa atcactggac gatgacaaaa
      361 gccggatcgg attctggctt caacatttcg caggaataca actaagagac attactgaat
      421 caaaaattta ttcagcaatg cagaaaatga cgaaccggcg tcatgaggaa aactggaaac
      481 tcagggcaga a
//
LOCUS       DAJTUI010000339          486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTUI010000339 DAJTUI010000000
VERSION     DAJTUI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.343"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..115)
                     /locus_tag="OO464_004348"
     CDS             complement(<1..115)
                     /locus_tag="OO464_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6229212.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADID"
     gene            complement(136..>486)
                     /locus_tag="OO464_004349"
     CDS             complement(136..>486)
                     /locus_tag="OO464_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6229213.1"
                     /translation="TVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRH
                     RKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNRR
                     LSESVPCMPHKKAG"
BASE COUNT          113 a          110 c          112 g          151 t
ORIGIN      
        1 ggtcaatatc ggcatcgttg aacagccgat gaccgccgtc tgtccgttgc ggtttcagca
       61 atccgtaacg cctctgccac gcgcgtaacg tgacagggat taaaggtaaa ccaattgatt
      121 ttgcaagttt ttattttacc ccgctttctt atggggcatg catgggacac tttcagatag
      181 tcttctgtta agaagttcta tctgttcgtg attgttgtct ttcatccatg ctccgtaaac
      241 attgaatacc atttgtgcgt ttgtgtggcc catctggctt gcgataaaac taggattagc
      301 tcctgcggca agtgaccagc atgcataggt atgcctggat tgatacgatt ttctgtgtct
      361 cagacctgcg cgttttaaga tacttgtcca tgactccctg atggagtcaa ccttatagtg
      421 cggtccggaa aactgccgct gttttattac ctgaggacta aaaacaaaag tgcatttatg
      481 cacagt
//
LOCUS       DAJTUI010000340          473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTUI010000340 DAJTUI010000000
VERSION     DAJTUI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.344"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..466
                     /locus_tag="OO464_004350"
     CDS             <1..466
                     /locus_tag="OO464_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229214.1"
                     /translation="TARILKDRSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTS
                     RDNSELFANDGTPTWLKEKRAKEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTS
                     RSWERIKGMDWPTIKAGRNALWIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          164 a          114 c          101 g           94 t
ORIGIN      
        1 caccgccaga atactaaaag atcgctccac ccgtattgat ggacaggtat tttcaaaccc
       61 aacatttaaa aactttcttc ctcatatcga gtctcttaac ctgggaggtg aacaccccgg
      121 catcgatacc tcacgggata acagtgaatt atttgccaac gacggtacac cgacatggct
      181 aaaagaaaag cgtgcaaaag aaatcgcact tgatgaaatt atcgagttgc tcaacaagca
      241 ccatgggggc accagtaacg acgccaaacg cgcaaaagct gatttgctgg aaaaaacatt
      301 cacctcccgc tcatgggaaa gaatcaaagg tatggactgg ccgacaatca aagctggtcg
      361 taatgcacta tggattgaac tggaaggcgt tgaatatgca ttcccggatc cacaaacaca
      421 aaacgaaacg caacaagcag gatacgatga gaatattccg gtgtaaaacc aag
//
LOCUS       DAJTUI010000341          470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTUI010000341 DAJTUI010000000
VERSION     DAJTUI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.345"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..>470
                     /locus_tag="OO464_004351"
     CDS             <1..>470
                     /locus_tag="OO464_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR6229215.1"
                     /translation="TDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVN
                     KPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFT
                     GICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFIS"
BASE COUNT          129 a          111 c           97 g          133 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg
//
LOCUS       DAJTUI010000342          470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTUI010000342 DAJTUI010000000
VERSION     DAJTUI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.346"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            172..466
                     /locus_tag="OO464_004352"
                     /pseudo
     CDS             172..466
                     /locus_tag="OO464_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          116 a          103 c          151 g          100 t
ORIGIN      
        1 ggaaattgcc cgctgcacgg agcgggaatt tgcgacaatt cctgccggta tcaacgccga
       61 cggcatgtat tgccctgaat gcggtacggt tcactggccg gatggcgtta ttccgccttt
      121 ctgatgcttc cccgttttgc cgatattttt cagcagggaa accgatggct taactggctg
      181 gagaagcagc cggtgcagat gagccgcctg gaacattatg ccggtcagga tgaaattggc
      241 ctgcgttaca acagccaccg gacaaaacga gaagaaaacc ttgtgatgag tggcgacgag
      301 tttatggaaa ggttctcctg gcatgtggcg gataaggggt tccggatggt gatacggggt
      361 cctgagtccg gtgaagcggc gattactgga agatgtggtg tacgccataa cggagacagt
      421 gagaaaaacg gcgaggcaaa tcacaaggag agggatgtat cagcagtgac
//
LOCUS       DAJTUI010000343          458 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTUI010000343 DAJTUI010000000
VERSION     DAJTUI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.347"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..415
                     /gene="istB"
                     /locus_tag="OO464_004353"
     CDS             <1..415
                     /gene="istB"
                     /locus_tag="OO464_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR6229216.1"
                     /translation="GYEAVRVGIKVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPR
                     LLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAM
                     LGRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT          131 a          108 c          117 g          102 t
ORIGIN      
        1 gggctatgaa gcagtccgtg taggtatcaa agttcgcttc acaacagcag cagatctgtt
       61 acttcagtta tctacggcac aacgtcaggg ccgttataaa acgacgcttc agcgtggagt
      121 aatggccccc cgcctgctca tcattgatga aataggctat ctgccgttca gtcaggaaga
      181 agcaaaactg ttcttccagg tcatcgctaa acgttacgaa aagagcgcaa tgatcctgac
      241 atccaatcta ccgttcgggc agtgggatca aacgttcgcc ggtgatgcag cactgacctc
      301 agcgatgctg ggccgtatct tacaccactc acatgtcgtt caaatcaaag gagaaagcta
      361 tcgactcaga cagaaacgaa aggccggggt tatagcagaa gctaatcctg agtaaaacgg
      421 tggatcaata ttgggccgtt ggtggagata taagtgga
//
LOCUS       DAJTUI010000344          458 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTUI010000344 DAJTUI010000000
VERSION     DAJTUI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.348"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..>458
                     /locus_tag="OO464_004354"
     CDS             <1..>458
                     /locus_tag="OO464_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229217.1"
                     /translation="TLISRDNKLVENFIDTLENWRDQKQIISVICKDGIKIDDSIITS
                     FKASKDVGISNGLRIQLTFQEINFKAIVGQTDVSAATGRTSTTNDGGATSKKNTGNTT
                     TSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASAN"
BASE COUNT          145 a           94 c          100 g          119 t
ORIGIN      
        1 tacccttatc tcacgagata ataagctggt ggagaacttc atcgacactc ttgaaaactg
       61 gcgtgaccag aagcagatta tttcagttat ctgtaaagat ggtattaaga ttgacgactc
      121 cattattaca agctttaaag cctctaaaga tgttggaatt tcaaacggac tgagaatcca
      181 gctaactttt caggaaatta acttcaaagc tattgtaggg caaactgatg tttcagcagc
      241 cactggacgg acttctacca cgaatgatgg cggtgctacc agtaaaaaga acactggcaa
      301 cactacgaca agtcttggaa gccctatgct gacctgtaaa gagcttttta gctattcggc
      361 aagtgagtta tcagatgaag cgttaaaagc ccgtgtaacg tgctctaaga gcgttagcgt
      421 taaaaatggt gagagcacct tcacagcctc agctaatg
//
LOCUS       DAJTUI010000345          453 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTUI010000345 DAJTUI010000000
VERSION     DAJTUI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.349"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
BASE COUNT          163 a           78 c           75 g          137 t
ORIGIN      
        1 ttgactcatg gttttctcta cttgtcaagg ataattaata cgactcacta tagggagaag
       61 ggacacaaaa agttttctct tctaggacaa ctaaccgtaa cttatcgcat cctttcaggg
      121 gcgggatgac caattacaga caccaagcgt tctacttaaa gtgaatgctt cctgtgtgct
      181 ctgggtcagt cgataagtga atagcaataa gacacaatca tcttaattat ggtctctagt
      241 ccctacaggg gcttaactta aagttaactt aaagacacaa ttaaagatta acttatagta
      301 ttacttaaag ttaacttaaa gacacaatta gagattaact ttaagtatta aataaaagat
      361 ttaattaaag accaattaaa gattaactct aagggaatac cttaaggggt gatgatattt
      421 ataataatca atcaccaaac ctataggtct tct
//
LOCUS       DAJTUI010000346          450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTUI010000346 DAJTUI010000000
VERSION     DAJTUI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.350"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..280
                     /locus_tag="OO464_004355"
     CDS             <1..280
                     /locus_tag="OO464_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6229218.1"
                     /translation="FKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRN
                     KHDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            277..>450
                     /gene="tnpB"
                     /locus_tag="OO464_004356"
     CDS             277..>450
                     /gene="tnpB"
                     /locus_tag="OO464_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6229219.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILW"
BASE COUNT          113 a          114 c          128 g           95 t
ORIGIN      
        1 gtttaaatgg aaaagccagt acgaggacgg cttactgagc gatgatgaca tacaggaatg
       61 catgcctgtc ccggtggcac tgactgatac gccggagccg accagaccag ttacaaatcc
      121 cttctggcgt aacaagcatg atgagcgccc tgagggggct cccggaaacg tcccacggtg
      181 cgagctgcat cttaaatcag gtgtggtaaa actgtttgac cctctcactc cggaactgtt
      241 acgggcgcta atccgcgaaa tgaaaggggg tatccgatga taacgctgcc gaccggtacc
      301 aaaatctgga tcatcgctgg catcacagat atgcgttgtg gcttcaatgg cctggcttcg
      361 aaggtgcaga acacgctgaa agatgacccg ttctccgggc atatcttcgt cttccggggc
      421 cgcagtggca aaatggtgaa aatactgtgg
//
LOCUS       DAJTUI010000347          442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTUI010000347 DAJTUI010000000
VERSION     DAJTUI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.351"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            213..>442
                     /locus_tag="OO464_004357"
     CDS             213..>442
                     /locus_tag="OO464_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6229220.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          140 a          105 c           61 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gg
//
LOCUS       DAJTUI010000348          441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTUI010000348 DAJTUI010000000
VERSION     DAJTUI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.352"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            1..149
                     /locus_tag="OO464_004358"
                     /pseudo
     CDS             1..149
                     /locus_tag="OO464_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(169..>441)
                     /locus_tag="OO464_004359"
     CDS             complement(169..>441)
                     /locus_tag="OO464_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HCR6229221.1"
                     /translation="SKSRSTGRILVLSLLATLSTIVMWLLGYHAENKGLHLKYQANSI
                     KSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT          111 a          106 c          103 g          121 t
ORIGIN      
        1 cgcgcgaagt gttgtatgac aatatgaaaa ctgtggttct gcaacgtgac gcatatcaga
       61 ccggtcagca ccggttccat ccttcgctgt ggcagttcgg caaggagatg ggcttctctc
      121 cccgactgtg tcgccccttc aggctgaggg atccccacaa aattactgct aataaaccag
      181 caccatattt cggtaggttc tggacagatg gtttagaact gtgctcagta ctgttcgtct
      241 taaaataagt ggcgagtgtc gaagaacatt cttcgctaac gtcagatatg agataacccg
      301 ccgggattta atgctgttag cctgatattt caggtgtaac cctttatttt cagcgtgata
      361 accgagtaac cacattacga tagtgctcaa cgttgccagc agactcagaa ccagaattct
      421 tcctgttgaa cgacttttgc t
//
LOCUS       DAJTUI010000349          441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTUI010000349 DAJTUI010000000
VERSION     DAJTUI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.353"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            320..>441
                     /locus_tag="OO464_004360"
     CDS             320..>441
                     /locus_tag="OO464_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6229222.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFG"
BASE COUNT           93 a          132 c          107 g          109 t
ORIGIN      
        1 gattcaggca ccagaatcac ccgacgcccc ggaggcgctg ccggggtgtc gttccatttc
       61 tgatgatgac ctgtgcgcct ggtgtacccg tctgctttac cgtcccggag aaatcagtct
      121 gtgcagactg agtatacagg caatggcatc tggccatccg tctgtgaccg gaacggttac
      181 gcgtacggct gtccggaatt tcagccgaat atcattcatc cctgatatta ccgggtcatc
      241 acggtcagtg atgaacgtga tgttgccagc tcccgacctt tactccctct catttattca
      301 cattacaagg atttcataca tgaaaacatt gtctcagaac accaccgctt cggcctgtgc
      361 accggagact ggcctgcaac aactggttgc cactcccgtc cctgatgaac agcgcatcag
      421 cttctggccg cagcattttg g
//
LOCUS       DAJTUI010000350          436 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTUI010000350 DAJTUI010000000
VERSION     DAJTUI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.354"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..162)
                     /locus_tag="OO464_004361"
     CDS             complement(<1..162)
                     /locus_tag="OO464_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HCR6229223.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYEL"
     gene            complement(167..>436)
                     /locus_tag="OO464_004362"
     CDS             complement(167..>436)
                     /locus_tag="OO464_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR6229224.1"
                     /translation="FGMVLAEASAFSVPVVATQIGGIPEVIQNNQTGTLLPASNKHAW
                     MCALNDFFNDPGRFYQMARLAKQDIEERFDINKTALKILTLAKQK"
BASE COUNT          137 a           96 c           77 g          126 t
ORIGIN      
        1 cagttcataa taatatcgaa tactgacaac cggatttata gaacatcgca aaatgtacat
       61 gaaccgaaat tttctgtgtt cccacttccc cggtagtgaa tctgaggaaa aaataatcct
      121 tttaaggaaa ccgataaatg ataagttact tatagaaaac atattgttac ttttgcttcg
      181 ctaatgtgag tatttttaac gcagttttat taatatcaaa ccgctcttct atatcctgtt
      241 ttgccaggcg agccatctga taaaaacgcc cagggtcatt aaaaaaatca ttcagggcgc
      301 acatccatgc gtgcttatta cttgctggta acaatgtccc ggtctggttg ttctgaataa
      361 cctcagggat tccaccaatc tgagtggcca ctacaggcac agaaaatgcc gatgcttctg
      421 ccagcaccat accaaa
//
LOCUS       DAJTUI010000351          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTUI010000351 DAJTUI010000000
VERSION     DAJTUI010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.355"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            35..434
                     /locus_tag="OO464_004363"
                     /pseudo
     CDS             35..434
                     /locus_tag="OO464_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAJTUI010000352          429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTUI010000352 DAJTUI010000000
VERSION     DAJTUI010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.356"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..297
                     /locus_tag="OO464_004364"
     CDS             <1..297
                     /locus_tag="OO464_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR6229225.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
BASE COUNT          146 a          103 c           82 g           98 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatc
//
LOCUS       DAJTUI010000353          426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTUI010000353 DAJTUI010000000
VERSION     DAJTUI010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.357"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..248)
                     /gene="yefM"
                     /locus_tag="OO464_004365"
     CDS             complement(<1..248)
                     /gene="yefM"
                     /locus_tag="OO464_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR6229226.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
BASE COUNT          103 a           99 c           86 g          138 t
ORIGIN      
        1 tcaataatgt ccttttccgt tcctttgcct gatttcaggc tatcgattga gtccatcaat
       61 ctccgggcgt tagcggggga acgcagtaga taagccgtct cttccagcga attgtattct
      121 tcgagtgaca tcagaacaca agcctctcca ttctgacgag taatgaggat cggggcatga
      181 tcttcaacgg ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt
      241 gtacgcatgt aaatctccta ttttgtacag ttcattgtac aatgataggt gttaattaac
      301 tatttattaa ttagtttgta gatcaagaca ttgccagcga gacgaaaaat cagactttgc
      361 tctttttggt gctgtcaagt tagaggacag tcctcttagc cccctccttt ccccgctcat
      421 tcatta
//
LOCUS       DAJTUI010000354          424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTUI010000354 DAJTUI010000000
VERSION     DAJTUI010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.358"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..>424)
                     /locus_tag="OO464_004366"
     CDS             complement(<1..>424)
                     /locus_tag="OO464_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR6229227.1"
                     /translation="LLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKG
                     GMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLM
                     TELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNT"
BASE COUNT          100 a          122 c          104 g           98 t
ORIGIN      
        1 gtattgttgg agagcttttt catcatcccc gccataaagc cgacttcacg accaccaacg
       61 ccgatatcac ctgccggaac gtcggtatcc gcgcccaggt ggcgatacag ttcagtcatc
      121 agcgtctggc aaaaacgcat cactttacct tcgctttttc ctttcggatc gaaatcgctg
      181 ccgcctttac caccgcccat cggcagggta gtcagggcat ttttgaaggt ttgttcaaag
      241 ccgaggaatt tgagaatgga aaggttaact gacggatgga agcgcatacc gcctttgtac
      301 gggccgatgg cagagctgaa ctgcacacgc catgcacggt tgacctgtat ctggttgcga
      361 tcatcaaccc ataccacgcg aaactggatc acgcgctccg gttcaaccag acgctccagt
      421 aatg
//
LOCUS       DAJTUI010000355          410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTUI010000355 DAJTUI010000000
VERSION     DAJTUI010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.359"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(51..>410)
                     /locus_tag="OO464_004367"
     CDS             complement(51..>410)
                     /locus_tag="OO464_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6229228.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
BASE COUNT           94 a          110 c           89 g          117 t
ORIGIN      
        1 gtgttcaccg ttggcgggta aggcaaaaca aattctatgc gttcggtcat ttatgctttc
       61 cacttcagaa cacccgaatt tctcgcgtgc attaaaaaac gaatcagcaa caacaactga
      121 ctaccgtgtt tttcttcaaa atcttttacc ccggcgtgta gttcgttatg gcatttacga
      181 cacagcggaa taacaaacaa atcgtcagcc tttgttccca tccctcccag tccatgacca
      241 atgatgtggt gcggatcatc tgcctgattg ccacacgtca tgcatttctg cgtttttacc
      301 cagcgcgtgt atacaggcat ctcttcccgt tgtggtttct ggcgctggag atactgagcc
      361 ggagattccg gatcaacggc aatgctgacc accgtctctt cctgtggtgg
//
LOCUS       DAJTUI010000356          408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTUI010000356 DAJTUI010000000
VERSION     DAJTUI010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.360"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..375)
                     /locus_tag="OO464_004368"
     CDS             complement(<1..375)
                     /locus_tag="OO464_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229229.1"
                     /translation="MVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFGGM
                     VRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEFDI
                     WNLNETWAFQEKLIFCEDESSLL"
BASE COUNT          125 a           97 c           87 g           99 t
ORIGIN      
        1 taacagactt gattcatcct cacaaaatat aagtttttcc tggaatgccc atgtctcatt
       61 gaggttccag atatcaaatt cgaagtcgtg ataacgaaaa cggatcccac caaacttatt
      121 aaaacgcagt tgtttgacgg gcagttcagc caatgtctgg aagagttcct cacgggagcg
      181 accgatgacg atatcaaggt ctgaagagaa tccatcgacg ccatacaagc ctaagtcacg
      241 caccatcccg ccaaaaatcc tggccggaca ggaacaggcc agagtatcaa gtaatactct
      301 ggctttctta tgctcatcac taagcgtaaa aaaatcggcg atacgctgtt caagcagttg
      361 ggtgtttgag accacattca tacagtgatt ttagctcatc gtgaaggc
//
LOCUS       DAJTUI010000357          400 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTUI010000357 DAJTUI010000000
VERSION     DAJTUI010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.361"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(153..332)
                     /locus_tag="OO464_004369"
                     /pseudo
     CDS             complement(153..332)
                     /locus_tag="OO464_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          113 a           78 c           97 g          112 t
ORIGIN      
        1 aggggcaggg gaatgtgccg tatcgatatg ctttggcaat tgcgtgtgcc gcagatggag
       61 atgaaatgcc aataaaaaag cctgagttcg atgagggacg aactcaggct ggtgttatgt
      121 tttacagtaa cacgcctcgg cgcgttggct gattagaact ggtagacgat accaactgcc
      181 agttggtcgt cggtgttcag tccatgagcg agaacataat cactcttatc aagcaggttg
      241 atctgataag cggtatatat gttcatattc ttattgaagt aataccaggt accgatttca
      301 atatagttca gacgatcaga atctttgtaa cctgtagact ggccccctga atctccagac
      361 aaccaatatc acttatttaa gtgatagtct taatactagt
//
LOCUS       DAJTUI010000358          398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTUI010000358 DAJTUI010000000
VERSION     DAJTUI010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.362"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            144..329
                     /locus_tag="OO464_004370"
                     /pseudo
     CDS             144..329
                     /locus_tag="OO464_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          117 a           95 c          103 g           83 t
ORIGIN      
        1 gtttacgctt accgcaggac aggtggtaac tgaaaaatag tttgctgagg cgactggaaa
       61 cagaagggca agatcgattc cgacagcagt gatggtcagc tacctcatcg ataactttgc
      121 aacagtgccc ttcttgaagc atcatgcttc atgaagaaaa actggcacgt catcaacgta
      181 aacaggcgat gtatacccga atggcagcct tcccggcggt gaaaacgttc gaagagtatg
      241 acttcacgtt cgccaccgga gcaccgcaga agcagctcca gtcgttacgc tcactcagct
      301 tcatagagcg caatgaaaac atagttttac aggggacatc tgacatcaca aatccccggg
      361 tggggatttg tgtataagag acagcccatg gaggaagg
//
LOCUS       DAJTUI010000359          398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTUI010000359 DAJTUI010000000
VERSION     DAJTUI010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.363"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..>398
                     /locus_tag="OO464_004371"
     CDS             <1..>398
                     /locus_tag="OO464_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6229230.1"
                     /translation="FSGAAASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVV
                     DRRNSNDFEIQRLNKFVVETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGS
                     GGTSFINGESKISTNSAYSHDLLSASLFEV"
BASE COUNT          121 a           80 c           86 g          111 t
ORIGIN      
        1 tttttctggt gcagcagcga gtggcgcagc taccgcaata ggaggttctt atagtgttac
       61 tgttgcacat aacaaaaaga accctcaggc cgcagaaact caggtttacg ctcagtcttc
      121 ttacaaggtt gttgacagaa gaaattccaa tgattttgag attcagaggt taaataaatt
      181 tgttgtggaa acagtaggtg ccaccccggc agagaccaac cctacaacat attctgatgc
      241 attagaacgc tacggtatag tcacttctga cggttcaaaa aaaatcatag gttttcgtgc
      301 tggctctgga ggaacatcat ttattaatgg tgaatccaaa atctcaacaa attcagcata
      361 tagccatgac ctgttaagtg ctagtctatt tgaggtca
//
LOCUS       DAJTUI010000360          397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTUI010000360 DAJTUI010000000
VERSION     DAJTUI010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.364"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
BASE COUNT          133 a           96 c           78 g           90 t
ORIGIN      
        1 tctaatcgtt actggtgaga aggcggaaga aaaagccagt tcttcgcccc taaataacag
       61 catgattcca gtccatgcaa aatctgattt ccagctcaca cataccgagc actgtacaaa
      121 aaaccgccag ccaaggcagt taagagtgta ttcccggggt ttgcttagaa tattttgtaa
      181 gttgtccgaa gagatattta caacaccaga atgatgcatt atcagtcccc tgccagaggt
      241 tcactacgta acctgaccga caatgcacca ttctggtgct gaaaaaagag cactggaact
      301 gcaacaaaac aacatcacag aacaggagat ggtaaggagt cagacattcc cacacaatat
      361 tgtgtcagtg caaataacaa ccttcgtctc aaatctc
//
LOCUS       DAJTUI010000361          390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTUI010000361 DAJTUI010000000
VERSION     DAJTUI010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.365"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            64..>390
                     /locus_tag="OO464_004372"
     CDS             64..>390
                     /locus_tag="OO464_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6229231.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPV"
BASE COUNT          132 a           71 c           90 g           97 t
ORIGIN      
        1 tgtgtcatga tggcttagat ttgaaccaac attagtgcat gggattttca gagggaaaaa
       61 atcatgttta ttgattcaga aaaacgactg aaacaacttt cagatgaggc aaagaaaaac
      121 accgaagatc tcgaagaagc aaagaagaat tcaaggttta cacaggtatc cccaaaaggt
      181 tgggaacgcg ttcgagagct gctgaaggat agccaaggca tatcagcact gaagctgtac
      241 tcatttttag cagagcatat cgatcctacg tgtggcgctg tcgttgcgga tcagcaattc
      301 ctagctgaaa aacttggagt tagcagaagc acaattattc ggtggctcaa ttacttagaa
      361 tcaaaaaatg cattagttag aatccccgtt
//
LOCUS       DAJTUI010000362          381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTUI010000362 DAJTUI010000000
VERSION     DAJTUI010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.366"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(273..>381)
                     /locus_tag="OO464_004373"
     CDS             complement(273..>381)
                     /locus_tag="OO464_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR6229232.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          106 a           87 c           77 g          111 t
ORIGIN      
        1 tcccgtaaag acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa
       61 ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattag
      121 gcacagctat ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa
      181 ttcactgtca tgctgatgct gcactgtggg gacagaatca ctcatgatga cttcgataat
      241 cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt
      301 gctccatttt ttaataaaaa atcaatcatt tttgaatctt tgcttttcat tgcattgtca
      361 agcatagtgt ccccactatt t
//
LOCUS       DAJTUI010000363          373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTUI010000363 DAJTUI010000000
VERSION     DAJTUI010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.367"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            56..373
                     /locus_tag="OO464_004374"
                     /pseudo
     CDS             56..373
                     /locus_tag="OO464_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          118 a           87 c          101 g           67 t
ORIGIN      
        1 ggtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca ttaccgaaca
       61 acaagaagcg tttatgacgc ttgagcagga gcagcaggtt aaaacccgta ctgccgaaca
      121 gaatgcacgt attgcggctt ttgaagctga acgtcgtcgt gaagcagagc agacgcgaat
      181 tctggctgaa cgacaaattc aggaaacgga gatcgagcgc gagcaggccg tccgctcaag
      241 aaaggttgag gctgaacgtg aagttcgtat taaagagatc gaacagcagc aggtcaccga
      301 aatcgccaac cagacgaaat cgatcgctat tgccgccaaa tcggaacaac agtcccaggc
      361 agaagcgcgt gct
//
LOCUS       DAJTUI010000364          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTUI010000364 DAJTUI010000000
VERSION     DAJTUI010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.368"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..311)
                     /locus_tag="OO464_004375"
     CDS             complement(<1..311)
                     /locus_tag="OO464_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6229233.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAJTUI010000365          366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTUI010000365 DAJTUI010000000
VERSION     DAJTUI010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.369"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
BASE COUNT           81 a           95 c          104 g           86 t
ORIGIN      
        1 tcccagaaac agaccgtgca ggagggatga ccggtgataa agtctgcaaa tacggactta
       61 taagggtgat tattggctct ggcgacggct ttcagaacct ctttcaccat tctggtgtgg
      121 actttctggt gctccaggtt gtgtgacatg ggaactcatt ctggatggtt actctgaaag
      181 ctcatattct gccacacccc gatttgcagc cgccaggctg ccgtggttca cagataaaac
      241 actctccagg aaacccgggg cggttcactg cgtgtctggg ttcgccagca tgagcgggat
      301 accgggggcg gttcacacga acataccaca gacagttgat tttggtacgc ccaggcgacg
      361 tccggc
//
LOCUS       DAJTUI010000366          357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTUI010000366 DAJTUI010000000
VERSION     DAJTUI010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.370"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            39..>357
                     /locus_tag="OO464_004376"
     CDS             39..>357
                     /locus_tag="OO464_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6229234.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          112 a           86 c           91 g           68 t
ORIGIN      
        1 gcctggcatc cggatataac ccgtgataaa acaagggaat gaacaatgaa ctccccgatg
       61 atattgagct gcttaaagcc atgttgcgta agcaacagag tcggcttcga cagtatgcct
      121 gtcaggtcgc gggctatgag caggaaattg aacggctgaa agcgcaactc gacaggttgc
      181 gtcgtatgtt gttcggccag agttcagaga aaaagcgtca taagcttgaa aatcagatcc
      241 gacaggcaga aaaacgactg tcggaactgg aaaaccggct gaacacagcc agaaatcttc
      301 tggaagatgc atcgtcagtc acagattcac ctgacaccag tcccccgtca gaaaacc
//
LOCUS       DAJTUI010000367          349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTUI010000367 DAJTUI010000000
VERSION     DAJTUI010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.371"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            173..297
                     /locus_tag="OO464_004377"
                     /pseudo
     CDS             173..297
                     /locus_tag="OO464_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; incomplete; partial in the middle
                     of a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT           94 a           76 c           91 g           88 t
ORIGIN      
        1 ggcaggagat tcgctctatg cagggttgtg agatgaattc aaaccggctg gcagcgccag
       61 tgatattcga agattcgtcc ggttgttacc cagtatgcat taaaaaccca gacattatgg
      121 acaatataac ggcttctaca acaaggaagt gcctgtttac catccgggtg taatgactca
      181 cctttgtggg cttttgcccg tcgcgctctt aacgttgaag acattctctg cctgccgatt
      241 caggactgcg acaaaagcca tatctggcta ctggtcaagg atgaccagcg tctggagtag
      301 gtgataagcc aaatagataa gctggaatat gtggtgaaag tgcaacgta
//
LOCUS       DAJTUI010000368          341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTUI010000368 DAJTUI010000000
VERSION     DAJTUI010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.372"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..169)
                     /locus_tag="OO464_004378"
     CDS             complement(<1..169)
                     /locus_tag="OO464_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229235.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSP"
BASE COUNT           94 a           88 c           93 g           66 t
ORIGIN      
        1 tcggggagag agtttcaaca gagatttgag tggtcataac gataactccg tacatttgga
       61 aattatcgcc accgacgacg ccaatcgaac tggtggcgaa ctgtgcaggg ttggcgtaac
      121 cgggtacgga aaccggcgag cctttcggct cccccacaca gcccgccata aatcgcgaat
      181 gtgactgtgc aaacgatatg aaaaaagacg cgggcgcgtc tcatatcgct ccgtaaacat
      241 ccgggacgcc aatcccgacg ccagattttg ctggcgcgtg aggaatatag ccccggataa
      301 cagattgagt caacaaacgg tttttagatc cccggaagag a
//
LOCUS       DAJTUI010000369          340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTUI010000369 DAJTUI010000000
VERSION     DAJTUI010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.373"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..>340)
                     /locus_tag="OO464_004379"
     CDS             complement(<1..>340)
                     /locus_tag="OO464_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6229236.1"
                     /translation="KSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPED
                     VIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYR
                     AKSLTFYGING"
BASE COUNT           76 a           97 c           88 g           79 t
ORIGIN      
        1 gccgttaatg ccgtaaaaag taagggattt ggctctgtac cgcggttctg ttgtctcagg
       61 gcgtgcatca gtccaccgga tgctgagcac cccttcaaac cgtgtgtcgg gtatgatgat
      121 gccgtagggg ccagcaacgg aatattcacc tggcagcgca ttccttaccc aggccaggaa
      181 atcactctta gtgtcaaaac ggataacatc ttcaggcaga aaagcacacc caaagccgaa
      241 tgcgccgggt atcgccagac ggccttttgt ccggtcgtaa atgtcgctct gtgcttccat
      301 cgtggccgca cttttcagcc ccagattatc ccgggacttc
//
LOCUS       DAJTUI010000370          337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTUI010000370 DAJTUI010000000
VERSION     DAJTUI010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.374"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
BASE COUNT           80 a           68 c          105 g           84 t
ORIGIN      
        1 aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca tcccaaaccc
       61 ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg gagattatct
      121 ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc tgctggcctg
      181 gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg gcggattgat
      241 atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg aaattctccg
      301 cgacagaaat cactgctcaa aatcggtgct gttgaaa
//
LOCUS       DAJTUI010000371          336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTUI010000371 DAJTUI010000000
VERSION     DAJTUI010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.375"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..255
                     /locus_tag="OO464_004380"
     CDS             <1..255
                     /locus_tag="OO464_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR6229237.1"
                     /translation="HYRNKIAIYLHWYQKKGIEVPQTQQGDIGAKDIPSWRRICKVLL
                     NNDYWCRALSFSPTKAKNYQRYNERIKGKRQEWGILCNND"
BASE COUNT          111 a           74 c           77 g           74 t
ORIGIN      
        1 cattaccgca ataaaatcgc catttatttg cactggtatc agaaaaaagg catcgaggtt
       61 ccacaaaccc agcaagggga cattggcgcg aaagatatcc cctcctggcg gcggatatgc
      121 aaagttttac tcaataacga ttactggtgt cgggcattat catttagtcc tacgaaagcg
      181 aagaactatc agcgttataa cgaacggata aaaggaaaac gtcaggaatg ggggatacta
      241 tgcaacaacg attaacgcag gatttaacgc aatttctcgc cagcttgccg gaagatgatc
      301 gcatcaaagc gattaatgaa atccgcatgg cgatcc
//
LOCUS       DAJTUI010000372          329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTUI010000372 DAJTUI010000000
VERSION     DAJTUI010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.376"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..160
                     /gene="xisR"
                     /locus_tag="OO464_004381"
     CDS             <1..160
                     /gene="xisR"
                     /locus_tag="OO464_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HCR6229238.1"
                     /translation="TGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWYNYPK
                     INQFIKDS"
     gene            162..>329
                     /locus_tag="OO464_004382"
     CDS             162..>329
                     /locus_tag="OO464_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HCR6229239.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHQISS"
BASE COUNT          107 a           61 c           79 g           82 t
ORIGIN      
        1 cactggtctg ggtgccagac aaattgaagc ataccgccag aactgttggg tggaaggctt
       61 ccacttcaaa cgagtatctc ctttagggaa gccagacagt aagcgaggga ttatctggta
      121 caactatccg aagataaatc agtttatcaa agactcatga tatgtctaaa ttaccaacag
      181 gtgtcgagat tcgaggtaaa tacattcgca tctggttcat gtttcgagga aaacgatgtc
      241 gggaaacatt gaaaggctgg gagattacta acagtaacat taaaaaggcc gggaatttaa
      301 gagcgttgat agttcatcaa atcagttcc
//
LOCUS       DAJTUI010000373          325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTUI010000373 DAJTUI010000000
VERSION     DAJTUI010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.377"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..>325
                     /locus_tag="OO464_004383"
     CDS             <1..>325
                     /locus_tag="OO464_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229240.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSG"
BASE COUNT           84 a           76 c           85 g           80 t
ORIGIN      
        1 tcggaatgaa ttatcaatta tatgtccgta attacagtcg taacaccaac tacgagatcg
       61 ttgctacggc tgatggcctg gatgtgctga acggcaagca aggttcgctg aataacaacg
      121 gctatattgt gaatgccgga gattcgttag tgattaaagg attccgcaaa gataaacata
      181 ctgaagcggc ttttcagttc gccaacgttg ctgattccta cgcagccaat tcggcgcaag
      241 gcgatgtgcg taataccggg gtaatcggct ttgccgcctt tgaattacaa ggcccggcgc
      301 aaaacgcgct gccgccgtgc tctgg
//
LOCUS       DAJTUI010000374          318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTUI010000374 DAJTUI010000000
VERSION     DAJTUI010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.379"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..>318)
                     /locus_tag="OO464_004384"
     CDS             complement(<1..>318)
                     /locus_tag="OO464_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6229241.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLL"
BASE COUNT           83 a          100 c           65 g           70 t
ORIGIN      
        1 agcaataaat cattctggat ctgctctgac aaccgtacgc cgccgcgcgt atctaacatc
       61 acctgacacc acgcctgcgc cattggcgga gaagcatatt tcaacatttg cgcaccgcag
      121 cccagcagga atagctgatg agtaatctct cgccccagtt cttcagctgg cttacgcagc
      181 tgctgctgta aacgacgaac cgcgcgatca aaatagcgat cctgcccttt cacttccaca
      241 aatgcttccg acaataagtc gtatacgccc gcttgcttat tgagaacgcg caacacatcc
      301 aggcacataa tattgccg
//
LOCUS       DAJTUI010000375          316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTUI010000375 DAJTUI010000000
VERSION     DAJTUI010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.380"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..>316
                     /gene="wcaD"
                     /locus_tag="OO464_004385"
     CDS             <1..>316
                     /gene="wcaD"
                     /locus_tag="OO464_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310888.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR6229242.1"
                     /translation="KRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIIL
                     SGSFSGVMTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLG
                     DLG"
BASE COUNT           78 a           64 c           69 g          105 t
ORIGIN      
        1 aagcgcacga cagcacttta tttcgaaccg gcatttttcg ctctggcatt aatctcaatt
       61 tggctcagca tcaaacagtt tggtatcaaa acgcctaaaa cagatgccct gattctcgca
      121 gggataatat tatccggatc gttttcaggg gttatgacct ttatcctgtt ttatttgctg
      181 gagtgggcat ttcaatatct gaataaagag gcgattaaga aaaaattacc gttagcattg
      241 atttctctgg ctgtattccg ggttggtgtg gtaatcgcgt ttccttatat ttccacccgt
      301 ctgggcgatt taggta
//
LOCUS       DAJTUI010000376          311 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTUI010000376 DAJTUI010000000
VERSION     DAJTUI010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.382"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(7..>311)
                     /gene="psiF"
                     /locus_tag="OO464_004386"
     CDS             complement(7..>311)
                     /gene="psiF"
                     /locus_tag="OO464_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR6229243.1"
                     /translation="VTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQALKGDAR
                     KTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACLKKAA"
BASE COUNT           70 a           79 c           73 g           89 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagcttgt tgcgttgctt gattattaca ttcacgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc a
//
LOCUS       DAJTUI010000377          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTUI010000377 DAJTUI010000000
VERSION     DAJTUI010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.383"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(30..>304)
                     /locus_tag="OO464_004387"
     CDS             complement(30..>304)
                     /locus_tag="OO464_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229244.1"
                     /translation="LNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLNDAVE
                     ELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT           79 a           72 c           69 g           84 t
ORIGIN      
        1 agcagcaaga tgattctgaa gttcaggaat tatttgcgca aggcccggca aatcagattc
       61 gatagcaact caagctgatg tgccgtttcc atgttcagcc acacataacc atagattggt
      121 gtttccacga ctttcaggtc atggtggtta tcgagcaact gacgcagctc ttccacagca
      181 tcgttcaatt tttccgtatt ggcaaaaacc ggctgcggat taccttcgta cagcgcatga
      241 acaaggctca gcagtatttg ctgcatcatg tgctgggtat cacaaagttt ttgcgcgttc
      301 aata
//
LOCUS       DAJTUI010000378          303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTUI010000378 DAJTUI010000000
VERSION     DAJTUI010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.384"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(50..190)
                     /locus_tag="OO464_004388"
     CDS             complement(50..190)
                     /locus_tag="OO464_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229245.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
BASE COUNT          108 a           40 c           43 g          112 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aac
//
LOCUS       DAJTUI010000379          301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTUI010000379 DAJTUI010000000
VERSION     DAJTUI010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.denovo.387"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
BASE COUNT           99 a           62 c           52 g           88 t
ORIGIN      
        1 cgccattgat gacccgcaat tctctgataa acgggcgcag gctgtttcat caatattttc
       61 ctcccgcgct aaagatcaca taatcttaac aagaatgtta aaaaacgctg gactcagaca
      121 gtagagtgtg tgttatggtt gactataaag tcagcgaagg aaatgcttct ggcttttaac
      181 agataaaaag agaccgaaca tgattcctgt tttcgtcaac aaacaataaa accctttaaa
      241 attaatgcgt tgaataaatt tacgttcatc ttttaatccc tgacacgtac cattgcatat
      301 t
//
LOCUS       DAJTUI010000380         5074 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.guided.182, whole genome shotgun sequence.
ACCESSION   DAJTUI010000380 DAJTUI010000000
VERSION     DAJTUI010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.guided.182"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..476
                     /gene="frdB"
                     /locus_tag="OO464_004389"
     CDS             <1..476
                     /gene="frdB"
                     /locus_tag="OO464_004389"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6229246.1"
                     /translation="GMKVEALANFPIERDLVVDMTHFIESLEAIKPYIIGNSRTADQG
                     TNIQTPAQMAKYHQFSGCINCGLCYAACPQFGLNPEFIGPAAITLAHRYNEDSRDHGK
                     KERMAQLNSQNGVWSCTFVGYCSEVCPKHVDPAAAIQQGKVESSKDFLIATLKPR"
     gene            487..882
                     /gene="frdC"
                     /locus_tag="OO464_004390"
     CDS             487..882
                     /gene="frdC"
                     /locus_tag="OO464_004390"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6229247.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            893..1252
                     /gene="frdD"
                     /locus_tag="OO464_004391"
     CDS             893..1252
                     /gene="frdD"
                     /locus_tag="OO464_004391"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6229248.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            1315..2448
                     /locus_tag="OO464_004392"
     CDS             1315..2448
                     /locus_tag="OO464_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6229249.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            2537..3070
                     /gene="blc"
                     /locus_tag="OO464_004393"
     CDS             2537..3070
                     /gene="blc"
                     /locus_tag="OO464_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6229250.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3067..3384)
                     /gene="sugE"
                     /locus_tag="OO464_004394"
     CDS             complement(3067..3384)
                     /gene="sugE"
                     /locus_tag="OO464_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6229251.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(3560..3706)
                     /gene="ecnB"
                     /locus_tag="OO464_004395"
     CDS             complement(3560..3706)
                     /gene="ecnB"
                     /locus_tag="OO464_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6229252.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(3817..3942)
                     /gene="ecnA"
                     /locus_tag="OO464_004396"
     CDS             complement(3817..3942)
                     /gene="ecnA"
                     /locus_tag="OO464_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6229253.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(3994..4560)
                     /gene="efp"
                     /locus_tag="OO464_004397"
     CDS             complement(3994..4560)
                     /gene="efp"
                     /locus_tag="OO464_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6229254.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            4602..>5074
                     /locus_tag="OO464_004398"
     CDS             4602..>5074
                     /locus_tag="OO464_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HCR6229255.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPEL"
BASE COUNT         1263 a         1309 c         1286 g         1216 t
ORIGIN      
        1 acggtatgaa ggttgaagcg ttagctaact tcccgattga acgcgatctg gtggtcgata
       61 tgacccactt catcgaaagt ctggaagcga tcaaaccgta catcatcggc aactcccgca
      121 ccgcggatca gggtactaac atccagaccc cggcgcagat ggcaaagtat caccagttct
      181 ccggttgcat caactgtggt ctgtgctacg ccgcgtgccc gcagtttggc ctgaacccag
      241 agttcatcgg tccggctgcc attacgctgg cgcatcgtta taacgaagat agccgcgacc
      301 acggtaagaa ggagcgtatg gcgcagttga acagccagaa cggtgtatgg agctgtactt
      361 tcgtgggcta ttgctccgaa gtctgcccga aacacgtcga tccggctgcg gccattcagc
      421 agggcaaagt agaaagttcg aaagactttc ttatcgcgac cctgaaacca cgctaaggag
      481 tgcaacatga cgactaaacg taaaccgtat gtacggccaa tgacgtccac ctggtggaaa
      541 aaattgccgt tttatcgctt ttacatgctg cgcgaaggca cggcggttcc ggctgtgtgg
      601 ttcagcattg aactgatttt cgcgctgttt gccctgaaaa atggcccgga agcctgggcg
      661 ggattcgtcg actttttaca aaacccggtt atcgtgatca ttaacctgat cactctgacg
      721 gcagctctgc tgcacaccaa aacctggttt gaactggcac cgaaagcggc caatatcatt
      781 gtaaaagacg aaaaaatggg accagagcca attatcaaaa gtctctgggc ggtaactgtg
      841 gttgccacca tcgtaatcct gtttgttgcc ctgtactggt aaggagcctg agatgattaa
      901 tccaaatcca aagcgttctg acgaaccggt attctggggc ctcttcgggg ccggtggtat
      961 gtggagcgca attattgcgc cggtgatgat cctgctggtg ggtattctgc tgccactggg
     1021 gctgtttccg ggtgatgcgc tgagctacga gcgcgttctg gcgttcgcgc agagcttcat
     1081 tggtcgcgta ttcctgttcc tgatgatcgt tctgccgctg tggtgtggtt tacaccgtat
     1141 gcaccacgcg atgcacgatc tgaaaatcca cgtgcctgcg ggcaaatggg ttttctacgg
     1201 tctggctgct atcctgacag ttgtcacgct gattggtgtc gttacaatct aacgcatcgc
     1261 caatgtaaat ccggcccgcc tatggcgggc cgttttgtat ggaaaccaga ccttatgttc
     1321 aaaacgacgc tctgcgcctt attaattacc gcctcttact ccacatttgc tgcccctcaa
     1381 caaatcaacg atattgtgca tcgcacaatt accccgctta tagagcaaca aaagatcccc
     1441 ggtatggcgg tggcggtaat ttatcagggt aaaccttatt actttacctg gggctatgcg
     1501 gacatcgcca aaaagcagcc cgtcacacag caaacgttgt ttgagttagg ttcggtcagc
     1561 aaaacattta cgggcgtgct tggtggcgac gctattgctc gaggggaaat caagttaagc
     1621 gatcccgcaa caaaatactg gcctgaactt accgctaaac agtggaatgg gatcacacta
     1681 ttacatcttg cgacctacac cgctggcggc ctgccattgc aggtgccgga tgaagtgaaa
     1741 tcctcaagcg acttgctgcg cttctatcaa aactggcagc ctgcatgggc tccaggaaca
     1801 caacgtctgt atgccaactc cagtatcggt ttgttcggcg cactggctgt gaagccgtct
     1861 ggtttgagtt ttgagcaggc gatgcaaact cgtgtcttcc agccactcaa actcaaccat
     1921 acgtggatta atgtacctcc cccagaagaa aagaattacg cctggggata tcgcgaaggt
     1981 aaggcagtgc atgtttcgcc aggggcgtta gatgctgaag cttatggtgt gaagtcgacc
     2041 attgaagata tggcccgctg ggtgcaaagc aatttacccc cccttgatat caatgagaaa
     2101 acgcttcaac aagggataca actggcacaa tctcgctact ggcaaaccgg cgatatgtat
     2161 cagggcctgg gctgggaaat gctggactgg ccggtcaatc ctgacagcat cattaacggc
     2221 agtgacaata aaattgcact ggcagcacgc ccggtaaaag cgattaagcc cccaactcct
     2281 gcagtacgcg catcatgggt acataaaacg ggggcgaccg gcggatttgg tagctatgtc
     2341 gcgtttattc cagaaaaaga gctgggtatc gtgatgctgg ctaacaaaaa ctatcccaat
     2401 ccagcgagag tcgccgccgc ctgtcagatt ctcaacgctc tacagtaaaa tttcatcggg
     2461 tccgaatttt cggacctttt ctgcgctttt ccttgctgtc atctacactt agaaaaaaac
     2521 cagtaaggaa acatttatgc gcctgctccc tctcgttgcc gcagcgacag ctgcatttct
     2581 ggtcgttgcc tgtagttttc ctacgccgcc gcgtggcgtg accgtagtga ataatttcga
     2641 cgccaaacgt tatctcggta cctggtatga gattgcccgt tttgatcacc gttttgaacg
     2701 tggactggaa aaagtcaccg caacatacag cctgcgtgat gacggcggcc tgaatgtcat
     2761 taataaaggc tataaccctg acagaggaat gtggcagcag agtgaaggga aagcgtactt
     2821 taccggcgca ccaactcgcg ctgcgctgaa agtgtcattc tttggtcctt tctatggcgg
     2881 ttataacgtt attgcactcg atcgggaata ccgccatgcg ctggtttgcg gcccggaccg
     2941 cgactacctg tggatactct cccgcacgcc aaccatttct gacgaagtga aacaggagat
     3001 gctggcagtc gcgacccggg aagggtttga tgtcagtaag tttatttggg tacagcagcc
     3061 cggtagttag tgagtgctga gtttcagacc aataatcccc aatacgatta acgccagact
     3121 cgccagacgc atcgggttag cggactcacc gagcagcaca atgccggtga tggccgcgcc
     3181 gactgcgcca atacccgtcc acacggcata agccgtccct accggtaacg atttcatcgc
     3241 ccaggcaagt agcgccatac tgactatcat cgccgtcacg gtaataacac tcggcgtcaa
     3301 acggctaaag ccgtgggtat atttcaggcc aacggcccat accacttcca gcagaccagc
     3361 aataactaag ataatccagg acatatcagg ctccggaaat ttggggccgt ccccggtgaa
     3421 aagaggcgtt tgcaggtcgt cctgcaaagc tgatggttga acggatattt tgccaggtgg
     3481 caggggaatt tcaactatta cctttatgac gatcgcagaa aaaacaaacg gcacgacact
     3541 gctgtcgtac cgttattgct tattgctgcg ctttcgttgc tgcgccagaa atcgcgttac
     3601 cgccatcaga aatgtcttca ccaacgccac gcgtggtgtt gcaggcagtt aatactgttg
     3661 aaagcaccag aacagaaaag atcgctgcaa ttgtcttctt caccataacg tcttcctttt
     3721 aggtaatgtt atttatgttt gcctatagca aattaataat agacaacatc gccaaaaatg
     3781 atggaaatct gatgattttt cggaagagaa gaaaaattag ctggcagcgc gagagatgga
     3841 gttaccgaga tgtttgatgt cttcgccgaa accgcaagcg gtgttacagc ccgtgagcag
     3901 cgtgctggca agcaaaacaa gaacgataag gcgtttcatc atctctgacc tgaataagtg
     3961 atggtgcagc ctgcaggccg caccacaacc gcattacttc acgcgagaga cgtattcacc
     4021 agagcgggta tccactttga tgacttcgcc gatttgtacg aacagcggaa ctttaaccac
     4081 agcgccagta gacagggtag ccggtttgcc gccagtacct gcggtatcac ctttcaggcc
     4141 cggatcggta tcaacgattt ccagttcaac gaagttcggc ggagtaacgg agatcggctg
     4201 accattccac agagttacga tacactctgc ctgatccagc agccatttag cattgtcgcc
     4261 aattgctttt gcatcagcag acagctgctc gaaagtttcg ttgttcatga agtgccagaa
     4321 ctcaccgtcg ttgtacaggt aagtcaggtt catatcgaca acatcagcgc cttcagcgga
     4381 atcagtagat ttgaaggttt tttctacgcg agtaccggtc agcagacgac gcagtttaac
     4441 gcgagcaaat gcctggcctt tacccggttt tacgaattca ctcgcttcaa ccgcgtaagg
     4501 ttcgccgtct aacatgattt taagaccagc acgaaaatcg ttgctatagt acgttgccat
     4561 aaggccctct gaaatttgtt aattggtagc taagccacaa aatggcgcat attgtaaccc
     4621 taaatacccc atccagagaa gattggttaa cgcaacttgc cgatgttgtg accgatcctg
     4681 atgaacttct gcgtcttttg aatatagacg cggacgaaaa actgttggcc ggacgcagcg
     4741 ccaaaaagct gtttgccctg cgtgtgcccc gctcatttat cgatcgcatg gagaaaggca
     4801 atccggacga tcctcttttg cgtcaggtac ttacctcgca agatgagttt gtcgtcgcgt
     4861 ccggattctc caccgaccca ctggaagaac agcacagcgt agtgcctggt ttgttgcata
     4921 aataccacaa ccgggcgctt ttgctggtca aaggcggctg cgcggtaaat tgccgctatt
     4981 gcttccgtcg ccacttcccc tatgccgaaa atcagggcaa caagcgtaac tggcagactg
     5041 cacttgagta tgttgctgcg catccggaac tgga
//
LOCUS       DAJTUI010000381         4228 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.guided.195, whole genome shotgun sequence.
ACCESSION   DAJTUI010000381 DAJTUI010000000
VERSION     DAJTUI010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.guided.195"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            <1..434
                     /locus_tag="OO464_004399"
     CDS             <1..434
                     /locus_tag="OO464_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HCR6229256.1"
                     /translation="NRHLIALRIMQQVKATGSPVLDVAHNFVSACRIGDQQGVLHRKG
                     ATPDDCGLVVIPGSRGDYSRLVQPVRSEETLHSLAHGAGRKWGRTECKGRLAAKYTAT
                     QLSRTELGSRVICRDKQLIFEEAPQAYKSAESVVQCQGRPD"
     gene            496..1110
                     /locus_tag="OO464_004400"
                     /pseudo
     CDS             496..1110
                     /locus_tag="OO464_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1167..2624)
                     /gene="pepD"
                     /locus_tag="OO464_004401"
     CDS             complement(1167..2624)
                     /gene="pepD"
                     /locus_tag="OO464_004401"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6229257.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2886..3344
                     /gene="gpt"
                     /locus_tag="OO464_004402"
     CDS             2886..3344
                     /gene="gpt"
                     /locus_tag="OO464_004402"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6229258.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3436..>4228
                     /gene="frsA"
                     /locus_tag="OO464_004403"
     CDS             3436..>4228
                     /gene="frsA"
                     /locus_tag="OO464_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6229259.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDH"
BASE COUNT         1007 a         1073 c         1139 g         1009 t
ORIGIN      
        1 ttaatcgcca cctgatcgct ttgcgcataa tgcaacaggt taaggccacc ggaagtccgg
       61 ttctggatgt ggcgcataac tttgttagcg cgtgtcgaat cggtgatcaa caaggggtct
      121 tacatcgtaa aggtgccacg cctgatgact gtggtctggt ggttattccc ggttcacgcg
      181 gtgattactc caggctggtt cagcccgtca ggagtgagga aacattgcat tcgctggcgc
      241 atggggctgg gcgtaaatgg gggcgcaccg agtgtaaagg gcgtctggca gcgaaataca
      301 cagcgacgca actctcgcgt actgaacttg gcagtcgggt aatttgtcgc gataaacaac
      361 tcatcttcga agaagcgcca caagcctata aatcggctga aagtgtggtg caatgtcagg
      421 gcaggccgga ttaattattc ctgtcgcgcg gctgcgtccg gtgctaacgc tcaaaaacag
      481 tggagggaaa aaaggatgat cttgctataa ctctcctcag cgcaggggcc ggaagaatgt
      541 tgtctcgcag tgaaaaaggc gctggacagg ctaattaaag aagctgcccg acaagacgtc
      601 gcggtaacgg tgctggaaac agaaacaggc cgctactctg acacgctgcg ttcggcgctg
      661 atttctctgg atggcgacaa cgcatgggcg ttaagcgaaa gctggtgtgg cactattcag
      721 tggatttgcc cgagcccgta tcgcccacat cacgggcgta aaaactggtt tctgggcatt
      781 gggcgtttta ccgctgatga gcaggagcaa tccgatgcga tccggtatga gacgctgcgt
      841 tcgtcggggc cgggcggtca acatgtcaat aaaaccgact cggcggtacg cgccacgcat
      901 ttggcatccg gtattagcgt gaaggttcag tcagagcgca gccagcatgc taacaaacgg
      961 ctggcgcgat tgctgattgc ctggaagctg gatcagcagc aataggaaaa tagcgcggtg
     1021 ctgaaatcac agcggcgaat gttccatcac cagattgaac gtggcaaccc gcgacgaacg
     1081 tttacaggga tggcttttat cgaaggataa tgaaggatga aactgccgga aggcgattaa
     1141 acgccatccg gcagcaaatc aaataattac ttcgccggaa tttctttcag cagttcagtc
     1201 agcagtgtcc agtaatgacc tacgctttcg atgtgaactt gctcatccgg agagtgtgga
     1261 ccggtgatag ttggcccgat agaaaccatg tccatttccg gatacggttt tttgaacaga
     1321 ccacattcca ggcccgcgtg gataatctgg atgttcggcg tcttgttgaa caggcgctga
     1381 taggtttcac gtaccagatg catcaccgga gaattagcgt ccggctgcca gccaggatat
     1441 gcgcctttcg cttcggtttt cgcgccagcc agtttaccca gcgaatccag catgctcacc
     1501 acgtagtctt taccgctgtc gattagtgaa cggatcaggc agtgaatttc tacgttattg
     1561 tcagtcatgg tcaccacacc gacgttcagg gaggtttcga ccacaccttt tgccacgtcg
     1621 gaattgcgga tcacaccgtt cggggtggcg ttcagcagac gaataaaggt atcgcgagat
     1681 ttcgcaatca gggcagcttt atcgttcgct acagagtcca gcaacaaggc cagatttttc
     1741 tctttctctg ccagctcgtt tttcaggatc tcctgatagg tattcaccag agatttcagt
     1801 gcgtcgactt tatcagctgc gacagcaatg gtcgcaaagg cttcacgcgg gatggcgtta
     1861 cgcagtgtgc cgccgttgaa atcgataagg cgcaggtcca gttcttctgc atgacccgcc
     1921 aggaagcgca ccagcagttt gttggcatta cccaggccaa cgtggatttc accgccggag
     1981 tgaccgcctt tcagaccttt taaggttaac ttgaaggttt caaaaccagc tggaaccgct
     2041 tcacgatcta aatgcaggtt ggaggtgaag tcgatacccc ccgcacaacc catgtagatt
     2101 tcaccttctt cttcggagtc ggtgttaatc agaatatcag cctgcaacca gttgctctgt
     2161 aagccgaacg caccgtccat accggcttct tcggtcatgg tcagcagcac ttccagcggg
     2221 ccgtgaacca cgttttcgtc agccagaacc gccagtgcag aggccatacc aatgccgtta
     2281 tccgcaccca gcgtggtgcc gcgcgcttta acccattcgc catcaatata aggctggata
     2341 ggatctttcg tgaagtcatg cacggtgtcg ttatttttct gcggcaccat atcgaggtgg
     2401 gcctgtaaga cgaccggttt acgattttcc atacctgcgg tagcaggttg acgaatcagg
     2461 atattaccta cctgatcgcg ttcgacatgg aaacctttct cttttgccca accaacaatg
     2521 tattcagcga gttgctcttc atgataggac gggtgaggaa tagaacagat tttggcaaaa
     2581 atatcccaca gcggctgtgg agataattga gacagttcag acacgttaag tctccttgtc
     2641 gatcacccgc aaaacagtat tgcaggtcac agggttagca gaaaatgttg tcaacacaag
     2701 acaggcttgc gagatatgta ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
     2761 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
     2821 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
     2881 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
     2941 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
     3001 gtctggtacc gggggcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt
     3061 gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag
     3121 gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt actgctgttg
     3181 cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc
     3241 gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatc gaacagccgt
     3301 gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg
     3361 cattgccggg cgttgttctt tttaacttca ggcaggttac aatagtttcc agtaagtatt
     3421 ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt
     3481 aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg
     3541 cagtcggctc ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg
     3601 tggatctggc gcggtattga cccacgtgaa atcctcgacg tccaggcacg tattgtgatg
     3661 agcgatgctg aacgtaccga cgatgattta tacgataccg tgattggcta ccgtggtggc
     3721 aactggattt atgagtgggc cacccaggcg atggtgtggc aacaaaaagc ctgtacggaa
     3781 gaagatccgc aactcagtgg tcgacactgg ctgcatgcgg ctacgttgta caacattgcc
     3841 gcctatcctc atctgaaagg agatgacctg gccgagcaag cgcaggcttt atcaaaccgc
     3901 gcctatgaag aggccgccca gcgtctgccg ggcacgatgc ggcagatgga gtttaccgta
     3961 cccggcggtg cgcccatcac cggctttttg catatgccga aaggcgatgg cccgttcccg
     4021 acagtattaa tgtgtggtgg tctggatgcg atgcagacgg actattacag cctgtatgaa
     4081 cgttattttg cgccgcgcgg cattgcgatg ctgactattg atatgccgtc ggtgggcttt
     4141 tcttcaaaat ggaagctcac ccaggactcc agcctgttgc atcagcacgt cttaaaggcg
     4201 ctgcctaacg taccgtgggt ggatcaca
//
LOCUS       DAJTUI010000382         3600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.guided.208, whole genome shotgun sequence.
ACCESSION   DAJTUI010000382 DAJTUI010000000
VERSION     DAJTUI010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.guided.208"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(49..1026)
                     /gene="intI2"
                     /locus_tag="OO464_004404"
                     /pseudo
     CDS             complement(49..1026)
                     /gene="intI2"
                     /locus_tag="OO464_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            1358..1831
                     /locus_tag="OO464_004405"
     CDS             1358..1831
                     /locus_tag="OO464_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6229260.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1926..2450
                     /gene="sat2"
                     /locus_tag="OO464_004406"
     CDS             1926..2450
                     /gene="sat2"
                     /locus_tag="OO464_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6229261.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            2508..3296
                     /gene="aadA1"
                     /locus_tag="OO464_004407"
     CDS             2508..3296
                     /gene="aadA1"
                     /locus_tag="OO464_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6229262.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            3372..>3600
                     /locus_tag="OO464_004408"
     CDS             3372..>3600
                     /locus_tag="OO464_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6229263.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSS"
BASE COUNT          996 a          809 c          862 g          933 t
ORIGIN      
        1 aaaagtatca ggggctactc agggctacat aaagatattt atctactttt actgattgat
       61 aagtagcatc agtccatccg caggactggt ggtgccggca aaatgctgac ccaacacatg
      121 cgtatagatt tgcgtggtct taacatcgtt atgccctaag agttcttgca cagtgcgaat
      181 atcacgcccc gcttgtaata gatgcgtagc aaacgagtga cgaaatgtat gacaagtgac
      241 acgcttgcta acgatgcctg ctttttgtac ggctgccttc aatgcctttc gcgcaacgga
      301 gtcatgcaga tgatggcggc ataatttgcc gttatacggg tggttgcaga gcgtgctgga
      361 gggaaagaca aacatccacg ccgcttgtcg ataagcagaa gggtatttgt gatctaaagc
      421 aaaaggcagc gatggcccta cgccttgtaa gttgtcgtct tgctgaataa gccgcgcttg
      481 ctcaatgagt tattttattg ctgggattag gcgcgtgggc agtaggctgt ttctgctttt
      541 cccaccctta ccgtcatgca cagtgatgca gccattatca aaatcaaaat ctttaacccg
      601 caaacgcaag cattcattaa tgcgcaaacc tgcaccatac agcagcgtaa aaataacttg
      661 gttgcgagta tccataacct gcaaaatgcg ttgcacttca tttgcagaga taacagaggg
      721 tagccgtcta ggcttgcttg cagggatata atcaatatcg cccaacggct gttgtaaaaa
      781 cctgttgtac aaaaaagcta gggcatttaa agcgattttc tgcgtgttta tggctacatg
      841 tctgctgttt gctaagctgg ataaaaacag cctgacctct tcactgccca tggtctgagg
      901 atgacgtttt ttgtgaaaca gaataaaacg cttaatccag tgcaggtaag ttttttcagt
      961 tttcagcgca taaccttttt gtcgcatatc cgtgcgtata gaatttaaaa atggactgtt
     1021 agacataaaa cgctccttgt cttgcaactg tctgcctata cagcctattc tagctgggat
     1081 ttaaaaaagt gcctgttttt tacgcctaga gatgcttgtt taccggtaga gttttaattt
     1141 aatgctaaat aaattaaaat gttatgagtt ctttgggtga gataatgtgc atcgtgcaag
     1201 caggatagac ggcatgcacg atttgtaata acagagtgtc ttgtattttt aaagaaagtc
     1261 tatttaatac aagtgattat attaattaac ggtaagcatc agcgggtgac aaaacgagca
     1321 tgcttactaa taaaatgtta acctctgagg aagaattgtg aaactatcac taatggtagc
     1381 tatatcgaag aatggagtta tcgggaatgg ccctgatatt ccatggagtg ccaaaggtga
     1441 acagctcctg tttaaagcta ttacctataa ccaatggctg ttggttggac gcaagacttt
     1501 tgaatcaatg ggagcattac ccaaccgaaa gtatgcggtc gtaacacgtt caagttttac
     1561 atctgacaat gagaacgtat tgatctttcc atcaattaaa gatgctttaa ccaacctaaa
     1621 gaaaataacg gatcatgtca ttgtttcagg tggtggggag atatacaaaa gcctgatcga
     1681 tcaagtagat acactacata tatctacaat agacatcgag ccggaaggtg atgtttactt
     1741 tcctgaaatc cccagcaatt ttaggccagt ttttacccaa gacttcgcct ctaacataaa
     1801 ttatagttac caaatctggc aaaagggtta acaagtggca gcaacggatt cgcaaacctg
     1861 tcacgccttt tgtaccaaaa gccgcgccag gtttgcgatc cgctgtgcca ggcgttaggc
     1921 gtcatatgaa gatttcggtg atccctgagc aggtggcgga aacattggat gctgagaacc
     1981 atttcattgt tcgtgaagtg ttcgatgtgc acctatccga ccaaggcttt gaactatcta
     2041 ccagaagtgt gagcccctac cggaaggatt acatctcgga tgatgactct gatgaagact
     2101 ctgcttgcta tggcgcattc atcgaccaag agcttgtcgg gaagattgaa ctcaactcaa
     2161 catggaacga tctagcctct atcgaacaca ttgttgtgtc gcacacgcac cgaggcaaag
     2221 gagtcgcgca cagtctcatc gaatttgcga aaaagtgggc actaagcaga cagctccttg
     2281 gcatacgatt agagacacaa acgaacaatg tacctgcctg caatttgtac gcaaaatgtg
     2341 gctttactct cggcggcatt gacctgttca cgtataaaac tagacctcaa gtctcgaacg
     2401 aaacagcgat gtactggtac tggttctcgg gagcacagga tgacgcctaa caattcattc
     2461 aagccgacac cgcttcgcgg cgcggcttaa ttcaggagtt aaacatcatg agggaagcgg
     2521 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     2581 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     2641 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     2701 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     2761 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     2821 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     2881 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     2941 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     3001 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     3061 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     3121 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     3181 ttgaagctag gcaggcttat cttggacaag aagatcgctt ggcctcgcgc gcagatcagt
     3241 tggaagaatt tgttcactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc
     3301 taacaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagagagc
     3361 tggggaagac tatgcgcgat ctgttgaagg tggttctaag cctcgtactt gcgatggcat
     3421 cggggcaggc acttgctgac ctgccaattg ttttagtgga tgaagctcgt cttccctatg
     3481 actactcccc atccaactac gacatttctc caagcaacta cgacaactcc ataagcaatt
     3541 acgacaatag tccatcaaat tacgacaact ctgagagcaa ctacgataat agttcatcca
//
LOCUS       DAJTUI010000383         2994 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603870
            SAMEA8900774-rid15817513.guided.220, whole genome shotgun sequence.
ACCESSION   DAJTUI010000383 DAJTUI010000000
VERSION     DAJTUI010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900774
            Sequence Read Archive: ERR6005215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478129.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 3,950
            CDSs (with protein)               :: 3,950
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 178 of 386
            Pseudo Genes (incomplete)         :: 107 of 386
            Pseudo Genes (internal stop)      :: 146 of 386
            Pseudo Genes (multiple problems)  :: 44 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900774-rid15817513.guided.220"
                     /serovar="S. flexneri 2a"
                     /isolate="603870"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-20"
     gene            complement(<1..454)
                     /gene="tetR(B)"
                     /locus_tag="OO464_004409"
     CDS             complement(<1..454)
                     /gene="tetR(B)"
                     /locus_tag="OO464_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6229264.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEH"
     gene            536..1741
                     /gene="tet(B)"
                     /locus_tag="OO464_004410"
     CDS             536..1741
                     /gene="tet(B)"
                     /locus_tag="OO464_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6229265.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1854..2447)
                     /gene="tetC"
                     /locus_tag="OO464_004411"
     CDS             complement(1854..2447)
                     /gene="tetC"
                     /locus_tag="OO464_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6229266.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2580..2951
                     /locus_tag="OO464_004412"
     CDS             2580..2951
                     /locus_tag="OO464_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6229267.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
BASE COUNT          814 a          541 c          616 g         1023 t
ORIGIN      
        1 gatgctcttg atcttccaat acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat
       61 ataatgcatt ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag
      121 tttcatactg tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac
      181 ttagtaaagc acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc
      241 cttctaaagg gcaaaagtga gtatggtgcc tatctaacat ctcaatggct aaggcgtcga
      301 gcaaagcccg cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct
      361 gggcgagttt acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc
      421 tgttaatcac tttactttta tctaatctag acatcattaa ttcctaattt ttgttgacac
      481 tctatcattg atagagttat tttaccactc cctatcagtg atagagaaaa gtgaaatgaa
      541 tagttcgaca aagatcgcat tggtaattac gttactcgat gccatgggga ttggccttat
      601 catgccagtc ttgccaacgt tattacgtga atttattgct tcggaagata tcgctaacca
      661 ctttggcgta ttgcttgcac tttatgcgtt aatgcaggtt atctttgctc cttggcttgg
      721 aaaaatgtct gaccgatttg gtcggcgccc agtgctgttg ttgtcattaa taggcgcatc
      781 gctggattac ttattgctgg ctttttcaag tgcgctttgg atgctgtatt taggccgttt
      841 gctttcaggg atcacaggag ctactggggc tgtcgcggca tcggtcattg ccgataccac
      901 ctcagcttct caacgcgtga agtggttcgg ttggttaggg gcaagttttg ggcttggttt
      961 aatagcgggg cctattattg gtggttttgc aggagagatt tcaccgcata gtcccttttt
     1021 tatcgctgcg ttgctaaata ttgtcgcttt ccttgtggtt atgttttggt tccgtgaaac
     1081 caaaaataca cgtgataata cagataccga agtaggggtt gagacgcaat cgaattcggt
     1141 atacatcact ttatttaaaa cgatgcccat tttgttgatt atttattttt cagcgcaatt
     1201 gataggccaa attcccgcaa cggtgtgggt gctatttacc gaaaatcgtt ttggatggaa
     1261 tagcatgatg gttggctttt cattagcggg tcttggtctt ttacactcag tattccaagc
     1321 ctttgtggca ggaagaatag ccactaaatg gggcgaaaaa acggcagtac tgctcggatt
     1381 tattgcagat agtagtgcat ttgccttttt agcgtttata tctgaaggtt ggttagtttt
     1441 ccctgtttta attttattgg ctggtggtgg gatcgcttta cctgcattac agggagtgat
     1501 gtctatccaa acaaagagtc atcagcaagg tgctttacag ggattattgg tgagccttac
     1561 caatgcaacc ggtgttattg gcccattact gtttgctgtt atttataatc attcactacc
     1621 aatttgggat ggctggattt ggattattgg tttagcgttt tactgtatta ttatcctgct
     1681 atcgatgacc ttcatgttaa cccctcaagc tcaggggagt aaacaggaga caagtgctta
     1741 gttatttcgt caccaaatga tgttattccg cgaaatataa tgaccctctt gataacccaa
     1801 gagggcattt tttacgataa agaagattta gcttcaaata aaacctatct attttattta
     1861 tctttcaagc tcaataaaaa gccgcggtaa atagcaataa attggccttt tttatcggca
     1921 agctctttta ggtttttcgc atgtattgcg atatgcataa accagccatt gagtaagttt
     1981 ttaagcacat catcatcata agctttaagt tggttctctt ggatcaattt gctgacaatg
     2041 gcgtttacct taccagtaat gtattcaagg ctaatttttt caagttcatt ccaaccaatg
     2101 ataggcatca cttcttggat agggataagg tttttattat tatcaataat ataatcaaga
     2161 taatgttcaa atatactttc taaggcagac caaccatttg ttaaatcagt ttttgttgtg
     2221 atgtaggcat caatcataat taattgctgc ttataacagg cactgagtaa ttgtttttta
     2281 tttttaaagt gatgataaaa ggcacctttg gtcaccaacg cttttcccga gatctcatct
     2341 attgaaacag cttgatagcc tttttcaaca aacaatattc gtgctgagtt aaccagtgat
     2401 tgataggtac tcttaaaatt ttcttgttga tgatttttat tttccatgat agatttaaaa
     2461 taacataccg tcagtatgtt tatggtatca tgatgatgtg gtcgtgacaa tcttaagaac
     2521 atttaggtta ttttatgtat attgaacagc attctcgcta tcaaaataaa gctaataaca
     2581 tccaattaag atatgatgat aagcagtttc atacaacggt tatcaaagat gttctattat
     2641 ggattgaaca taatttagat cagtctttac tgcttgatga tgtggcgaat aaagcgggtt
     2701 ataccaagtg gtattttcag cggctgttca aaaaagtaac aggggtcaca ctggctagct
     2761 atattcgtgc tcgtcgtttg acgaaagcgg ctgttgagtt gaggttgacg aaaaaaacta
     2821 tccttgagat cgcattaaaa tatcaatttg attcccaaca atcttttaca cgtcgattta
     2881 agtacatttt taaggttaca ccaagttatt atcggcgtaa taaattatgg gaattggagg
     2941 caatgcactg agagggtgat gctgccaact tactgattta gtgtatgatg gtgt
//
